LOCUS       DATMKH010000001         1066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_962930,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000001 DATMKH010000000
VERSION     DATMKH010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_962930"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            212..>1066
                     /locus_tag="VNV63_00005"
     CDS             212..>1066
                     /locus_tag="VNV63_00005"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HXC61028.1"
                     /translation="MSKHHEDEFRELAAGYFQGRITRRRFIQQATKLGISAALLSRMV
                     PATYAAGENLVDSSPESPNEAPITKERIEFLKTKPYKGTTINVLVLKATVGDCLKYHA
                     PKWAELTGGHVNVAEVPIDTLHQQIFSDLSTGLGRYDTYMTGAWFYGDFFTGKEPYIV
                     EIEPFLKDPKHSYWEPDQWIPAMKQLYTWQGKIYGVLFDGDCQILYYRKDIFEKPENQ
                     QKFKEKLGYDLPSPPKTMKEMHDLASFFTGWDWNGDGQSDWGITLHAKVNEQGFFHFL
                     TLSAPYVIS"
BASE COUNT          272 a          287 c          275 g          232 t
ORIGIN      
        1 gccaactagc gccttcttga cgcaatagtc tgaggcgtga tagcttctgg ctttccccca
       61 gaaatcctga ctcgctccgc gttggcgaat ccgacgtgaa ccaattggtt cccgccaatc
      121 ttcgcgctgt tgaacagagg cagagattag gaaaccagag caaaacggat ttcgagggga
      181 acggttaacc acacgataga ttacccccct tatgtccaaa catcacgagg acgagttcag
      241 agaattagca gctgggtatt ttcaaggaag aatcactcgg cgtagattta ttcagcaggc
      301 caccaaactt ggaatttccg ctgccctgct cagccggatg gtgcccgcga cttacgcggc
      361 aggtgaaaac ctggtcgact cttcacccga gtcgcccaat gaagctccca tcactaagga
      421 acgcatcgag ttcctgaaga ctaagccgta caagggtacc actatcaatg tccttgtgct
      481 caaggcgacg gttggagact gcctgaagta tcacgcgcca aaatgggcgg agctaaccgg
      541 cggtcatgtg aacgtcgccg aggttccgat cgacactctc caccagcaga tcttttcgga
      601 tctatcgact gggctcggcc gatatgacac ctatatgacc ggggcgtggt tctacggaga
      661 ctttttcacg ggaaaggaac cctacatcgt cgaaatcgag ccgttcctca aggatcccaa
      721 acactcctat tgggaaccgg atcaatggat accggcgatg aagcagctct atacctggca
      781 gggaaagatt tatggcgtgt tgttcgacgg agattgccaa atcctctatt accggaagga
      841 tatcttcgag aagccggaaa atcagcagaa gttcaaggag aagcttggct acgacctgcc
      901 aagtcctccc aagaccatga aagagatgca cgatctagcg tcctttttca cgggctggga
      961 ctggaacggc gatggacaga gtgactgggg gatcaccctg catgcaaagg ttaacgaaca
     1021 gggatttttc cactttctga ctctttccgc gccctacgtc atctcc
//
LOCUS       DATMKH010000002         3053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_322177,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000002 DATMKH010000000
VERSION     DATMKH010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3053
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322177"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /locus_tag="VNV63_00010"
     CDS             <1..689
                     /locus_tag="VNV63_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HXC61029.1"
                     /translation="IAKPIEGRVVNKETGEPVEGAVVVAQWILATPPEGKENDYWIVI
                     ETVTDAEGKYSIPGWGPEGRPRSRWLDKYDPALEVFKPGYWPEGLANRPEGVLGAIGI
                     NPRGTRVRESYWNGKDIQFIPFKIGETIGIKELYPFETSDERIAMSQEKWAEEIDSVQ
                     NDVNWGGVGKKWTAQDWLRIKSLVRLIHDACLKLPSQLQSNLRGLPDEYKKLLLGDAP
                     ACPAESSSSG"
     gene            907..>3053
                     /locus_tag="VNV63_00015"
     CDS             907..>3053
                     /locus_tag="VNV63_00015"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HXC61030.1"
                     /translation="MSPYESKHDKDQTNITFEYLEMQNQNRPVSWKKKLLWALGGLAI
                     LLSITVGLVIWIFSKDLPPIEALRAYQPSLISRVYGDDQRLLGQFYIEKRILVPLNQM
                     PKELFQAVIATEDTRFYEHGGIDFLGILRAFLTNLESLKIRQGASTITQQLARSLFLS
                     PERSFKRKVKEALLARKMEKALTKDEILEIYLNQIYFGHGAYGVQSAARTYFRKDVGE
                     LSLAECAFLAGLPRAPNDYSPYFNPEKAKQRQGVVLRRMTDEGFITEQQYRKAYQENL
                     YFQKLERDQELAPYLMEYVRQYLIARYGESAVYRGGLNVYTTINLDMQHAAVKALRDG
                     LREVDKRQGYRGPVAKKSPSEVTQMNAEGNVVTAAELREGDVLEGLVTATNPESATVI
                     AGGLKGKILAADLAWARKRLTGPDLLRDVEFSRSAQPPDILQAGDVIKVGIKKLDKKG
                     AQVLFTLEQDSIVEGALLSLDPKTGAIKAMVGGYDFKRSEFNRAVTAKRQPGSAFKPF
                     IYAAAMDAGLSPSTIVVDAPVIFDDPVLDKVWKPVNYEGKFYGPISLREALAHSRNVA
                     TVRLLERVGIKNAIEMARKAGITSPLTYDLSLALGSSSLSLSEMVSGYGVFANQGIRA
                     EPFSILLVTDSNGQSLENHVPLSQQVITKETAYMVTNMMEDVIQKGTGWRAKTLGKQL
                     AGKTGTTNDFTDAWFVGFAPNLTAGVWVGFDDQR"
BASE COUNT          756 a          726 c          881 g          690 t
ORIGIN      
        1 atatagcgaa gccgattgag gggcgggtgg tgaacaagga aacaggtgaa cccgtcgaag
       61 gagcggtcgt cgttgctcag tggatcctcg ctacaccgcc ggaaggtaag gagaatgact
      121 actggatcgt gatcgagacc gtaacggatg ccgaagggaa atacagcatt cccggctggg
      181 gacccgaagg gcggccccgg tcccgatggc tcgataaata tgatccggcg cttgaggtct
      241 tcaagccggg ctattggccg gaggggctgg ccaacaggcc tgaaggagtt ttgggagcca
      301 tcgggatcaa tcctagagga acgagggtaa gggagtccta ctggaacggc aaggatatac
      361 agtttatacc gtttaagatc ggagaaacga ttggaataaa ggagctgtat ccatttgaaa
      421 cctcggatga gcggatcgcg atgtcgcaag aaaaatgggc ggaggagatc gattcagttc
      481 agaacgatgt caattggggc ggtgtaggga aaaaatggac agctcaggat tggctgagga
      541 tcaaaagtct ggtccggttg attcatgatg catgtctcaa gctgccgtcg cagctccaat
      601 ctaatctccg cggactgccg gatgaataca agaaactcct tttaggagat gcgcccgcgt
      661 gtcccgccga atcttcatct tctggataac cgaaaactgt cttgacgata cgcgtatgac
      721 agaatcctga aggtcgcgcg caccattgcc gatcttgccc gatcttgccg aggccattcg
      781 gtaccgtagc ctagaccatc tgattgttac ctgctatcca tgcagtcata ccatcctgcc
      841 tcggtatttg gtcttccgaa agttatgata taatgtttac gctattatga tgagggaaag
      901 gatctgatgt ctccgtacga aagcaagcac gacaaagatc agacgaacat cacctttgaa
      961 tatctcgaga tgcaaaacca aaaccgcccc gtgtcctgga agaagaaact gttgtgggcg
     1021 ctgggcggct tagcgattct gctctcgatc actgtaggcc tggtgatctg gattttctca
     1081 aaggatcttc ctccgatcga agccctccga gcctatcagc ccagcctgat cagccgggta
     1141 tatggggatg atcaacggct gctcggccag ttttatattg agaaacggat tttggttccg
     1201 ctcaaccaga tgcccaaaga actctttcag gccgtgatcg ccaccgaaga tacgcgtttt
     1261 tatgaacatg gcggcatcga ttttctcgga atccttcggg catttttgac caatctggag
     1321 tcgttaaaga tccgtcaagg cgccagcacg atcacacaac aactggcccg gtccctgttc
     1381 ttatctccgg agcgaagttt taaacgtaag gtcaaggagg ctttactggc tcgaaagatg
     1441 gaaaaagcat tgaccaagga tgagatcttg gaaatctatc tcaatcagat ctatttcggc
     1501 cacggggctt atggggtgca atccgcagcc cgtacttatt ttaggaagga tgttggagag
     1561 ctatcgcttg cggagtgtgc ttttctggca ggacttccaa gggctccgaa tgactactcc
     1621 ccttatttca atccggagaa ggccaagcag cggcaggggg tggttctccg aaggatgacg
     1681 gatgaggggt ttattacgga gcaacagtat cgaaaggcat atcaagagaa tctctatttc
     1741 cagaagttag agcgggacca ggagctcgcc ccttatttaa tggagtacgt acggcagtat
     1801 ttaatcgccc gttatgggga gtctgcggtc tatcgtggcg ggctgaatgt ctacaccaca
     1861 atcaatttag atatgcagca tgccgcagtc aaggcgctca gagatggatt gcgggaagtg
     1921 gacaaacggc agggctaccg cgggccggtt gcgaagaagt caccctcgga ggtaacccag
     1981 atgaatgccg aaggcaacgt tgtgacagcg gctgagctga gagagggaga tgtcttggaa
     2041 gggctggtga ccgccacaaa cccggagtcg gcgaccgtta tagcgggagg acttaaagga
     2101 aagattctag ccgctgacct cgcctgggca aggaaacgtc tcaccggtcc ggatttactt
     2161 cgagatgtag agttctcacg ttccgcacaa ccgccggaca ttctccaggc cggtgatgtg
     2221 atcaaggtcg gaatcaaaaa attggataaa aaaggcgctc aggttctttt cacgctggaa
     2281 caggattcaa tcgtggaagg cgccttgcta tccttggacc caaagactgg ggccatcaag
     2341 gcgatggtgg ggggatatga ttttaaacgg agcgaattta atcgggcggt cactgcaaaa
     2401 cggcagcccg gctcggcctt caagcccttc atctatgcgg ccgcgatgga tgccggcctt
     2461 tctccctcta caattgtagt agatgctccg gtcatcttcg atgatccggt gcttgacaag
     2521 gtctggaagc cggtcaacta cgaggggaaa ttttatggtc cgatcagcct ccgtgaggcg
     2581 cttgcacact cccgcaacgt ggcaacggtc cggctcctgg aaagggtagg tatcaagaac
     2641 gcgatcgaga tggcacggaa agcgggaatc acgagccctc tcacttacga tctctctctc
     2701 gctttgggct cttcaagcct gagcctatcg gagatggtga gcggttacgg cgtgtttgcc
     2761 aatcaaggca ttcgagcgga acctttttcc atcttattgg tcacggattc caacggtcag
     2821 tcgctggaga accatgtgcc gctctcgcag caggtgatca caaaggagac agcttacatg
     2881 gtgaccaata tgatggagga tgtgattcag aaaggaaccg gctggcgcgc aaagacattg
     2941 gggaaacagt tggcgggtaa gaccggtacg accaatgatt ttacggatgc ctggtttgtc
     3001 ggttttgccc caaacctaac tgccggcgtc tgggttggat ttgatgatca gcg
//
LOCUS       DATMKH010000003         1762 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_323348,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000003 DATMKH010000000
VERSION     DATMKH010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323348"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1363)
                     /locus_tag="VNV63_00020"
     CDS             complement(<1..1363)
                     /locus_tag="VNV63_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXC61031.1"
                     /translation="MNEERAPEYGGFGSEPSTPLEYLERIIASLPPGDPTRSELIDLR
                     SNFAEQEEMISEARHTIEKLDEVIKKVTSPANRIGTYLGTPNKETAQIVVGGSDYYCN
                     VDPRINLSRLKKGTRVLVNEAYVIVGDLGYDLSGPVTKITELLGTDRLRVGMEHGMQA
                     VVLQRSQDLMKETLKVGDEIRVDPNYRVAIEVLSQSKSEEYYLETVPELPWEKVGGQE
                     EALSAIKDAIELPLLHADLFAKFRHQTPKGFLLYGPPGCGKTLIGKATAYNLTRQLKE
                     KTGEDMQQFFMHIKGPEILNMWVGESERMVREIFATAREKRKEGFLPFVFIDEAESIL
                     GTRRAGRYSNVLSTLVPMFCTEMDGIESLNEVVIILASNRADLIDPAILRPGRIDRKI
                     KVDRPDREAAREIYRIYLSEDLPYDPELIKQTGSVPQAVSHLIDKVVEAQFSHRDENR
                     FLDV"
     gene            1567..>1762
                     /locus_tag="VNV63_00025"
     CDS             1567..>1762
                     /locus_tag="VNV63_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61032.1"
                     /translation="MLDTLKGLKREVVSPVSREQYVDAFNWMLLARTAEEKLASLWRA
                     GKISGGVYLGKGQEAISAALG"
BASE COUNT          394 a          474 c          444 g          450 t
ORIGIN      
        1 tgacgtcgag gaacctgttt tcgtcccgat gggaaaattg ggcttcaacg accttatcga
       61 tcagatggga aaccgcctgc ggaacacttc cggtttgttt gatcagctcc ggatcatagg
      121 gaagatcctc gctcaaatag attcgataaa tctctcgggc tgcttcgcgg tctggccggt
      181 ccaccttgat ttttcgatcg attcgccccg gccgcagaat ggccggatcg atcaggtcgg
      241 cccggttgga cgccaagatg atgaccactt cgtttaacga ttcgatcccg tccatttcgg
      301 tgcagaacat cgggaccaga gtcgataaaa cattcgagta gcggcctgcg cggcgagtcc
      361 caagaatact ttcggcctcg tcaataaaaa caaacggcaa gaagccttct ttccgttttt
      421 ctcgggcagt tgcgaaaatt tcgcggacca tgcgctcgga ctctcccacc cacatattca
      481 ggatttccgg acccttgata tgcataaaga actgctgcat atcttcgccg gttttttcct
      541 tcaattgccg ggtcaaattg tacgcagtag ccttcccgat caaagtctta ccgcacccgg
      601 gcgggccgta cagaaggaag cctttgggtg tctggtgccg aaattttgca aacaggtccg
      661 catgcaaaag gggcaattcg atcgcatcct tgatggcact gagagcttcc tcctggccac
      721 cgactttctc ccacggtaat tcgggcactg tctcgaggta gtactcttcc gatttcgatt
      781 gactcaagac ttcaatggcg actcgataat tcgggtcgac ccgaatttcg tcaccgacct
      841 tgagcgtttc cttcatcagg tcctgggatc gctggaggac aacggcctgc atcccgtgct
      901 ccatgccaac ccgcagacga tccgttccca gtaattcagt gatcttggtg accgggccgg
      961 acaaatcgta ccccaggtct cccacgatca cgtacgcttc attgaccagg actcgcgttc
     1021 ctttttttaa tctcgaaagg ttgatcctcg gatcgacgtt gcagtaataa tcagaaccgc
     1081 cgacgacaat ctgagcagtc tctttatttg gagtgccgag ataggtccca atgcgattcg
     1141 ccggggaagt gactttcttg atgacctcgt cgagcttttc gatcgtatgc cgagcttcgc
     1201 tgatcatctc ttcctgctcg gcgaagttgg atcgtaaatc gatcaactcg cttcttgttg
     1261 ggtcacctgg tggaagactg gcaatgattc tctcaaggta ttcgagagga gttgaaggct
     1321 cagaaccaaa accaccatat tcgggcgctc tctcttcgtt catctgtact tcttaatcaa
     1381 tccgcgtctc gactataaac ggattccgaa ttcgtagcaa acctggactt gaccctgcgg
     1441 ctgaatttca ttcgatcaat atgtctctgg acatcaccaa cgaaagaact attcgtttag
     1501 ctcgacggta tcatcatgag taagaaagcg ttgcatcact ttcgggatta ctgatattga
     1561 tcgcttatgt tagacaccct caaggggctg aagagggaag ttgtttcccc tgtttcgcgg
     1621 gaacaatacg tcgacgcctt taactggatg ctactggcgc ggacggccga agaaaaattg
     1681 gccagtctgt ggcgggccgg gaagatttcc ggcggcgttt acctgggcaa gggacaggag
     1741 gccattagcg ccgcgctcgg cg
//
LOCUS       DATMKH010000004         1034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_644202,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000004 DATMKH010000000
VERSION     DATMKH010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644202"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..768)
                     /locus_tag="VNV63_00030"
     CDS             complement(<1..768)
                     /locus_tag="VNV63_00030"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HXC61033.1"
                     /translation="MGADTQPAPLVLTRINETFYQATAALVVEVLTRTGQPVTVVDGS
                     HTSAYKAIQDGTADLCVGFWLPTGHEKAWNQVKDSAVELSTIYEGARFYWAVPSYIPE
                     SEVSSIEDLRKPSVNAKMIKTIRGLSLDASITTESIDAIGAYDLGKAGYRVIPGGFEP
                     WKEALGSAIETKAWIVEPLWEPYYFNRIYSLRPLKDPKNVFKGRNRVILAAHNGVREK
                     LPKKTIDALKRMRVRLEDITDMDVDINVNSVTPEVAAR"
     gene            complement(831..>1034)
                     /locus_tag="VNV63_00035"
     CDS             complement(831..>1034)
                     /locus_tag="VNV63_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HXC61034.1"
                     /translation="IGDHFTMGPKGAAEAVKLVSPNRVVPMHYGTFLPMMTGTPGQFK
                     EELKALGLEKKLFPMIVGQTERF"
BASE COUNT          230 a          297 c          254 g          253 t
ORIGIN      
        1 tctcgcagca acctccggtg taacggaatt cacattgata tcgacgtcca tatctgtgat
       61 gtcttccagg cgcactcgca tccgcttcaa cgcgtcaatt gtctttttcg ggagcttctc
      121 ccgaaccccg ttatgtgcgg cgagaatgac gcgattcctg cccttgaata cattcttggg
      181 atctttcagc ggcctcaacg aataaatccg attgaagtaa tagggttccc ataagggttc
      241 gacaatccat gccttggttt cgatcgcgct acctagcgct tccttccagg gctcaaaccc
      301 acccggaatc acccgataac ccgccttccc gaggtcgtat gctccaatcg catcaatgga
      361 ctctgtcgtg atcgaagcat ccaggctgag accccgaata gtcttgatca tttttgcatt
      421 gaccgagggc ttcctcagat cttcaattga agacacctcc gactcgggta tgtaactcgg
      481 tacggcccaa tagaatcgtg ctccttcata gattgtcgac aattcgacgg ctgaatcctt
      541 gacctgattc cacgctttct cgtgtccagt cggtaaccag aatccgacgc agagatccgc
      601 agttccatcc tgaatcgcct tgtaggcgct ggtatgcgag ccgtcgacca cagtgaccgg
      661 ttggcccgtc cgagttaaga cctccaccac cagagcagct gtcgcttgat agaacgtctc
      721 attgatccgc gtcaaaacaa gcggggcggg ttgcgtgtca gcccccaagg agatttcagg
      781 tgcgaaacct gcgaatagaa gtgcgagggc aaacgacggg agtcttagca ttagaagcgc
      841 tcggtctgac cgacgatcat ggggaagagt tttttctcaa gacccagtgc cttcagctct
      901 tccttaaatt gtccgggggt gccagtcatc atcggcagga aagttccgta atgcatcgga
      961 accactctgt tgggtgaaac cagctttacg gcctcggccg ctccctttgg tcccatcgtg
     1021 aagtgatcgc caat
//
LOCUS       DATMKH010000005         1204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4495976, whole genome shotgun sequence.
ACCESSION   DATMKH010000005 DATMKH010000000
VERSION     DATMKH010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1204
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4495976"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            934..>1204
                     /locus_tag="VNV63_00040"
     CDS             934..>1204
                     /locus_tag="VNV63_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61035.1"
                     /translation="MVFLRDAEKTLGGQFGQSESMEREQLPRPLLIFDGDCSFCRAWV
                     EYWKDLTGDHVGYTPLQEVGDRFPRVSHQEFTSAVQLVLNDEVRSG"
BASE COUNT          266 a          322 c          322 g          294 t
ORIGIN      
        1 aggttgtcac gaacgtactt gacccgagga ccccgaggac caaactatct gttatcccgc
       61 aggctttttc ttaaggaagc aaggggcaaa gatttgtgac ccgatcacag acgcgccccc
      121 agtgcactgg atagtatccc agcgacctcg tttaagtatt ggcgatcctt aattcgccct
      181 gaggcggccc gaatcttttc acgccgcctc ttttttctcc tcagtaaaag ccgaaggcgc
      241 cagtacgtgt tagcaggccc aagtcttccg cctacacctg ccaacagacg aacgcccagg
      301 gaccaaaaag aaaaaaccgc tccgaagtgc gggagcaggt cacagctaac ccggtcacga
      361 ttcccttgtg acttgtgggg aacaaagtgg agaacaaaaa ttgccgattg agagctgatc
      421 tgcccgattc cggcgatccg gtccgcttga aaacatccga tccgattgag aaccgggtca
      481 atccatccat cttgaaaacc gctgtacggg aaaccgtacc gatggttcga atccctctcc
      541 ctccgccatt aattgctgtg ttttgagtct ttctttcttc ccgatttcta accgggctgt
      601 ccctctaggt tgtgattggc gtccacgatg gaaaaagagc aaccaggaac ccgcctagtt
      661 tcggaaagac tgggccactt ctagctaccg atgctgatcc taagttttct tacttttatc
      721 tatattgtta ttactgagta cccgataagg ggccgcagac gtcggggctg cagactctac
      781 ttttcgaggc tcggtgtttt ggtgttttaa atgcgcgatc gtctatccgt cggtgtcatt
      841 ccgctttcag gtctttcgta gaaatagtgg ggccgttctg agatccttga gaatccgcct
      901 ttaccaggag tggctcagcg gaagacgtgg ttcatggttt tcctacgaga cgctgaaaaa
      961 acgctcggag gccagttcgg gcagagtgag tctatggaac gagagcaact gccgcgtccg
     1021 cttctcatct ttgatggaga ctgttcgttc tgccgggcgt gggtggagta ttggaaggac
     1081 ttgacgggtg accacgtggg ctatacgccg ctccaggagg tcggcgaccg cttccctcgg
     1141 gtgtcgcatc aggagttcac gtcagcagtc caactcgtcc tgaacgacga ggtgcgaagc
     1201 ggtg
//
LOCUS       DATMKH010000006         1113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_647600,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000006 DATMKH010000000
VERSION     DATMKH010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_647600"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="VNV63_00045"
     CDS             complement(<1..214)
                     /locus_tag="VNV63_00045"
                     /inference="COORDINATES: protein motif:HMM:NF012442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HXC61036.1"
                     /translation="MVLSWQMKKSDLVREMTGKNGGNSGDAADQVDRAVHRIIRALRG
                     GKSARLPGLGTINPGKHWTFKPERNDS"
     gene            381..527
                     /locus_tag="VNV63_00050"
     CDS             381..527
                     /locus_tag="VNV63_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61037.1"
                     /translation="MKKLLVFALGLGIALGTVSFSFAQDTTSTTKKKTKKKKADKTDT
                     AATK"
     gene            559..708
                     /locus_tag="VNV63_00055"
     CDS             559..708
                     /locus_tag="VNV63_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61038.1"
                     /translation="MKRLLVTALGLGIALGTISFSFAQDTTDTTKKMQKKKKKADKTD
                     TAATK"
     gene            874..>1113
                     /locus_tag="VNV63_00060"
     CDS             874..>1113
                     /locus_tag="VNV63_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HXC61039.1"
                     /translation="MNISVKGEYALQAIFDLAANPGAEPVKIAEIARRQKIPQKFLEL
                     ILASLKQGGFVESRRGAEGGYMLARRAESITVGDVL"
BASE COUNT          262 a          316 c          285 g          250 t
ORIGIN      
        1 aagagtcatt tcgctccggt ttgaaagtcc agtgttttcc ggggtttatc gtgcccagtc
       61 cgggaagccg ggcggacttc ccgccgcgca gtgcgcgaat gattcgatgg accgcccgat
      121 ccacctgatc ggcggcgtca cccgaatttc cgccattttt cccggtcatt tctctcacga
      181 gatccgattt cttcatctgc caggagagta ccataggccc gtccagctca atcgctaagc
      241 tgttgattgt attgaatcaa acccctgtga acggttaatt aagtgaattt aatcacagtt
      301 taaggtgggg ttcatctccc gttggctatc attctcaaga cggcatctcg tcccaatcat
      361 tctaaggaga atcacaaagc atgaaaaagc ttctcgtatt tgcgcttggc ttgggcatcg
      421 ccctcggcac cgtttccttc tcgttcgctc aggacacgac ctcgactacc aagaagaaga
      481 ccaagaagaa aaaggctgac aagaccgaca cagccgccac gaagtagttt ccgttacaac
      541 tttcacggag cctgacatat gaagagactt ttagtaaccg cgctcggcct cggtatcgcc
      601 ctcggaacca tctccttctc gttcgcccag gacaccacgg acacgaccaa gaagatgcag
      661 aagaaaaaga agaaggctga taagaccgac acagccgcca cgaagtaatt tcccagggct
      721 ggccgcctcg ggaaaccggg gagggtcagc ccttcagttt ttccccccgc acttccctcc
      781 atcttttccc caaaacatcg tatttgttga cggatcgcct caaaccctct aatctctagt
      841 tttgcagggg gaattgagcg aggcacttcg ccgatgaaca tttcggtcaa gggtgaatac
      901 gcccttcagg ccattttcga tctggcggca aaccccggcg cggagccggt gaaaatcgcc
      961 gagattgcgc gcagacagaa gattccacag aagtttctgg agttgattct ggccagtctc
     1021 aaacagggtg gattcgtcga atcgcgccgg ggcgccgagg gcggatacat gctggcgagg
     1081 cgcgccgaat cgataacggt gggcgatgtc ctg
//
LOCUS       DATMKH010000007         2189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1290583, whole genome shotgun sequence.
ACCESSION   DATMKH010000007 DATMKH010000000
VERSION     DATMKH010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1290583"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="VNV63_00065"
     CDS             complement(<1..430)
                     /locus_tag="VNV63_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013309931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61040.1"
                     /translation="MKIQNSPDSFLNIKDAPPSLVWGYCNTPLQGFFNIDKPGGCTSH
                     DVVAKVRRLLHIQKVGHAGTLDPLATGVLPICVGKGTKVVEYLLESDKEYRATLRLGE
                     ETDTLDAAGKILRRSDKRVTEDEFRSVLGEFIGRIDQIPPM"
     gene            complement(466..1431)
                     /locus_tag="VNV63_00070"
     CDS             complement(466..1431)
                     /locus_tag="VNV63_00070"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HXC61041.1"
                     /translation="MKKEMEKVIKEIRDRNSFLVTAHMNPEADAMGSSLALAWSLRKL
                     GKQAQVINRDPLPKVLSFLPHQGILTQTAQIASPPDALFILDCGDIERTGYFDGVTPP
                     PFPVINIDHHVTNKNFGTVNWVDPDAAASAELVYDLDKALGIDLTPEISICLYTALLS
                     ETGFFAYSNTSPKVLKIAAELIERGVDPWAVAQKVRENSIGRLHLLGELLLRLEQTPD
                     GKIAWLSVTQEDFKRTGTTAEDMEEFVSYPRSLKGVEVALVFREVDRETCKVSFRSRD
                     RVDVSALAQQFGGGGHRKASGCTVKGGLKEVQTRVIQVVKKGLPS"
     gene            complement(1428..1778)
                     /gene="rbfA"
                     /locus_tag="VNV63_00075"
     CDS             complement(1428..1778)
                     /gene="rbfA"
                     /locus_tag="VNV63_00075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HXC61042.1"
                     /translation="MESYKRTDRLGDQFRMEIADILLKKSKDPRIGFITVTGVEVSVD
                     LRHAKVFVSSMEAPAALERTFRGLSRSAGFIRSELGKRLKLRRIPELTFHQDRSLEQA
                     AHLFEVMEGIPKEP"
     gene            complement(1838..>2189)
                     /locus_tag="VNV63_00080"
     CDS             complement(1838..>2189)
                     /locus_tag="VNV63_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009382224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HXC61043.1"
                     /translation="ADVKAAMEGLLEPTLKERVLGRAEVRQLFQTPKVGMIAGAYVTD
                     GIVSRNSAGVRVLRDQQVVYQGKINSLRRFKDDVREVQTGYECGIGVENFNDLKTGDV
                     LEVYTIDKIAAKLA"
BASE COUNT          493 a          648 c          548 g          500 t
ORIGIN      
        1 acattggagg aatctggtcg atccggccga taaactcgcc cagcacggaa cgaaattcat
       61 cttccgtgac gcgcttatcg gaacggcgga ggatctttcc cgctgcatcc agtgtatcgg
      121 tctcctctcc taagcgaaga gtcgcccggt attccttatc cgactccagc aggtattcaa
      181 ccaccttcgt tcccttcccc acacagatcg gaagcacacc cgtcgccaat gggtccaagg
      241 tcccggcatg ccccaccttt tgtatatgca gcagccttcg aaccttggcc accacatcgt
      301 gagaggtgca gccgccgggc ttgtcgatgt tgaagaaacc ctgaaggggc gtattgcaat
      361 atccccatac aagagacggc ggcgcatctt ttatgttcaa aaagctatca gggctgtttt
      421 gaattttcat agagggttgg aaaattccaa gtgaggtacg agtcatcaag aaggcaatcc
      481 cttcttcacc acctgaatca cacgtgtctg aacctccttc aatccgcctt tgacagtgca
      541 gcctgaggcc ttccggtgtc cgccgccgcc gaactgttgg gccagcgccg atacgtccac
      601 ccggtcccgg gaacggaaac tcaccttaca ggtctcacga tccacctcgc gaaacaccag
      661 agctacctcg acccctttga gggaacgggg ataactcacg aactcttcca tatcctcggc
      721 cgtcgtcccg gtcctcttaa aatcctcttg cgtcacggaa agccaggcga tctttccatc
      781 gggtgtctgc tccaaccgaa gaagcaactc gcccaggaga tggagacggc cgatggaatt
      841 ttcacgaacc ttctgtgcca cagcccaggg gtccactccg cgctcgatga gttccgccgc
      901 gatcttcaag accttgggag aggtgttcga gtaggcaaag aatccggttt cgctcaggag
      961 ggctgtatag aggcagatcg agatctccgg tgttaaatca atacccaacg ccttatccag
     1021 gtcgtagacc aattcagccg atgcggctgc atcgggatca acccagttca cggtcccgaa
     1081 atttttattc gtgacatgat gatcaatatt gatcaccgga aaaggcgggg gagtcacccc
     1141 gtcaaagtag ccggtccgct cgatgtctcc gcagtccagg atgaaaagag catcgggggg
     1201 agaagcgatc tgtgcggtct gggttagaat gccttggtgg ggaaggaacg aaaggacctt
     1261 cgggagcgga tcgcgattga tcacctgagc ctgcttaccc aacttccgga gagaccaggc
     1321 caatgccaga gaagagccca tcgcatccgc ttccggattc atatgggcgg tgactaagaa
     1381 agagttgcgg tcccggatct ccttgattac tttttccatc tcttttttca tggctctttt
     1441 ggtattccct ccatcacctc gaagagatgt gcagcctgct ccagcgacct gtcctgatga
     1501 aaagtcagtt ccggaatacg ccggagcttt aaccgcttgc ccaactcaga ccggatgaac
     1561 ccggctgatc ggctcagtcc ccggaaggta cgctccaacg cggccggcgc ttccatgctg
     1621 ctcacgaata ccttggcatg acggagatct accgagacct cgacgccggt cacggtaatg
     1681 aatccgatcc gcggatcttt gctttttttt aacaagatgt ccgcgatctc cattcgaaat
     1741 tgatcgccca aacgatcggt gcgcttataa ctttccatgt ctccaggttg ggtcaagaat
     1801 ttcattagtg agtgaaagac acattttaca atatcagcta agccagcttt gcagcgatct
     1861 tgtcgatcgt ataaacctcg aggacgtctc ctgttttcaa gtcattgaag ttctccaccc
     1921 cgatgccgca ttcgtaaccg gtctggactt cacgcacatc gtctttgaaa cgtctcagcg
     1981 agttgatctt tccttgatag acaacctgct ggtctcgaag cacacgcacc ccggcgctgt
     2041 tgcgggagac gatgccgtcc gtcacatacg cgccggcgat catgcccact ttcggggtct
     2101 gaaagagctg ccggacctcg gcccgaccca gcacgcgctc ctttaaagta ggctcgagca
     2161 acccttccat cgccgccttt acatccgcg
//
LOCUS       DATMKH010000008         1307 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4178654, whole genome shotgun sequence.
ACCESSION   DATMKH010000008 DATMKH010000000
VERSION     DATMKH010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4178654"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="VNV63_00085"
     CDS             complement(<1..335)
                     /locus_tag="VNV63_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018509474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HXC61044.1"
                     /translation="MKKAAFGLLLMIFLLAGFLLAQADDRVEIKMILKDNRFVPDEIR
                     VQAGKSVTLSITNSDATAEEFESFALNREKLIPAGKTITIFLPKLQPGTYEFGGEFHP
                     KTARGRLIV"
     gene            complement(332..787)
                     /locus_tag="VNV63_00090"
     CDS             complement(332..787)
                     /locus_tag="VNV63_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HXC61045.1"
                     /translation="MSKKEEQLLKSHLLQNHLRLTKQRSAIVQTFLEMQPHVTTEELH
                     QELVKRRYPVGIATIYRTLNLLCQWGVAQQRQFGAGPALYELHENLHSHRHHDHLICT
                     SCGKIIEFKAPKIEELQNKVATQYRFTIHHHKHELYGLCAGCKEKVPAS"
     gene            962..>1307
                     /locus_tag="VNV63_00095"
     CDS             962..>1307
                     /locus_tag="VNV63_00095"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HXC61046.1"
                     /translation="MKNLLATPPVSIQKMEEVWQLYREDLALVETQIKKTLTSSVPLV
                     NEVTQHLLTSGGKRIRPLLLLMTSRLCGPLTKETILLAALIEAVHTAALLHDDVLDEA
                     EIRRGEKAVRSIW"
BASE COUNT          296 a          326 c          350 g          335 t
ORIGIN      
        1 ttcacaatta aacggccgcg ggcggttttg gggtggaact cccccccgaa ttcgtaggtg
       61 cctggttgaa gtttcggcag gaagatcgtg atggtcttgc cggcagggat gagtttctcc
      121 cggttgagag caaaactctc aaactcttct gccgtggcat cggaattggt gatgctcagc
      181 gtaaccgact tcccggcctg gacacgaatc tcatccggaa caaaacggtt gtccttgagt
      241 atcatcttga tctccacgcg gtcgtcggcc tgcgcgagga gaaatcccgc caggagaaag
      301 atcatcaaga gtaacccgaa agcagccttt ttcatgacgc cggcaccttt tctttacagc
      361 ctgcacaaag accgtagagt tcatgtttgt gatggtggat ggtaaaacga tactgggttg
      421 ccaccttgtt ctgcaactct tcgatcttgg gtgctttgaa ctctatgatc ttcccgcagc
      481 tcgtacagat gaggtgatca tgatggcggt gactgtgaag attctcatgg agttcataca
      541 gtgccggccc tgcaccaaac tgacgttgtt gggccactcc ccactggcat agaaggttga
      601 gggttcggta gatcgttgcg atcccgaccg ggtagcgacg cttcacaagt tcttgatgga
      661 gttcttcggt cgtcacgtga ggctgcatct ccaaaaaggt ctgaacgatc gccgagcgct
      721 gtttggtcag acgcagatgg ttctgcagca gatggctttt taaaagttgt tcttcttttt
      781 tactcatgtt ctttactcat gtcgacacca gatgatattg agattgatta tcattataat
      841 gtggtgcttt ccggattgtc aagagcataa aaatataccg gccaggttta accaaaaact
      901 ttgcgttgga acgcggaatt ttgggcttga caaaccaccc acgctggcct agtattttca
      961 catgaaaaac cttctcgcca cgccaccggt gtctatccaa aaaatggaag aggtctggca
     1021 gttgtaccgg gaggacctgg cccttgtgga gacccagatc aaaaagacgc tgacatcgag
     1081 tgttcctctg gtcaacgagg tcacacagca tcttttgaca agcgggggca agcggatccg
     1141 tcctcttctc ctcttgatga cctcgaggct ttgcggtccc ttgaccaaag agacaatcct
     1201 gctggcggcc ttgatcgagg cggtccacac cgcggctctt cttcatgacg acgtccttga
     1261 tgaagctgag atacggcgcg gtgagaaggc ggtccgaagc atctggg
//
LOCUS       DATMKH010000009         1272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2574602, whole genome shotgun sequence.
ACCESSION   DATMKH010000009 DATMKH010000000
VERSION     DATMKH010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2574602"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="VNV63_00100"
     CDS             complement(<1..471)
                     /locus_tag="VNV63_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020672336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXC61047.1"
                     /translation="MESVPEPAGEEKRADAGTEKGQWSILAVVCVGTFMALLDAFIVN
                     VALPTIQSTLNANAGELELVIASYSLTYAVFLITGGRLGDIFGRKKLFVLGMAVFTIA
                     SATCGLAPSPEILIGSRALQGSGAALMYPQILSIIQVTFSGQKRVAALGIFAGVN"
     gene            complement(554..>1272)
                     /locus_tag="VNV63_00105"
     CDS             complement(554..>1272)
                     /locus_tag="VNV63_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61048.1"
                     /translation="AAIHFMVPDYPDSRKDILPLQQVIEMGHSHEVPIIVDAAGQTYP
                     IELLSRFTKMGADLVCFAAKYVQGANSAGWVCGKKELIETVSLHSFIGQEAGGYDPRP
                     GFYRSVGRGYKLDRQEIVGTLAALQRWIKMDHEKERIEPALRKIEYLQRSLGNVQGVT
                     FSSWPETLVDGIGYHQLGVQVHFEKKSPDEVAEITRKLREGDPSVWVYYHGGNSIEIN
                     VLYLANGDEEIIAEKIKELI"
BASE COUNT          283 a          359 c          307 g          323 t
ORIGIN      
        1 gttgaccccg gcaaatatcc cgagcgccgc aacgcgcttc tggccgctaa acgttacctg
       61 tattattgaa agaatctgcg ggtacatcaa agcggcgcca gagccctgta atgctctgga
      121 tccgatcagg atttcaggag agggtgcgag gccgcatgtc gcggaagcta ttgtgaacac
      181 tgccatccct aatacgaaca acttttttct gccaaagatg tcgcccagcc ggcctccagt
      241 aatcagaaag accgcgtagg tcagtgaata gctggcgatt acaagttcga gctctcccgc
      301 gttcgcgttg agtgttgatt gaatggtcgg gagcgcaacg ttcacgataa aggcgtcaag
      361 caacgccatg aaagtgccta cgcaaacgac tgcaagaatt gaccactgcc ctttctccgt
      421 accagcgtct gcccgcttct cttcgccagc gggttcaggt acggattcca aagtaaactt
      481 cggctcgagc tacgggagat cgggggtaat caatgttagg gcaaatagtt ccttttgtta
      541 tcggaatttg gagctagatc agctctttga ttttctctgc gataatttct tcgtctccgt
      601 ttgcaagata cagcacattg atctcgatac tatttccacc gtgatagtac acccagaccg
      661 agggatctcc ctctcggagt tttcgagtaa tttctgcgac ctcatcggga ctctttttct
      721 caaaatgaac ctgtacgcct aactggtgat agccgattcc atcaacgagc gtttctggcc
      781 atgaggagaa agtcactcct tggacattac ctaaagatct ctgcaaatac tcgatctttc
      841 gcaatgccgg ctcgatcctc tccttctcgt gatccatctt aatccatctc tgcaatgccg
      901 ctagggtgcc cacgatctcc tggcggtcga gcttgtagcc tctgccgacg ctcctgtaaa
      961 atccgggcct cggatcgtag ccccccgctt cctgcccaat gaaactgtgc agcgatacag
     1021 tttcgatcag ctcttttttc ccgcacaccc agccggcgga atttgctccc tgcacgtact
     1081 ttgccgcgaa gcacaccaga tccgcaccca tttttgtaaa cctgctgaga agctcgatgg
     1141 gatacgtttg tcccgccgcg tccacgatga tcgggacttc atgactgtgt cccatctcta
     1201 tgacctgttg gagggggagt atgtctttcc gggaatccgg ataatcgggc accatgaaat
     1261 ggatcgccgc cg
//
LOCUS       DATMKH010000010         1117 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2574821, whole genome shotgun sequence.
ACCESSION   DATMKH010000010 DATMKH010000000
VERSION     DATMKH010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2574821"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1071
                     /locus_tag="VNV63_00110"
     CDS             <1..1071
                     /locus_tag="VNV63_00110"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HXC61049.1"
                     /translation="QSLIQQLRKENERLKKELFQTAKELSFFINTGKALTSTIEFQKV
                     LRVIMEKAQKLIKCEAWSLFLIDETTQELYFGLVKGKMNKKVRQFRLKIGEGIAGWVV
                     KNAMPIMVPDVRKDHRFSRHIDRVTGFKSRSILCVPIVSKKKTIGALEMINKHHVMFE
                     KKDLDLLTKLVDQAAIALERSSLYQKMADLAITDDLTKLFNFRYLDQTLDTELKRSQR
                     YGAQISLIFFDMDYFKLVNDRHGHLMGSKVLIEVAQILINSLRDVDIVSRYGGDEFVV
                     VLPETRVDTATKIVHRIHKAIREHEFLKEEGLNLHLSASYGVSGYPDHAKTKKDLIRL
                     ADQAMYKAKSSGRDRICVVGEE"
BASE COUNT          289 a          279 c          298 g          251 t
ORIGIN      
        1 cagagcctca tccagcaact ccgaaaagag aacgagcgat taaaaaaaga gcttttccaa
       61 accgccaagg agctctcctt tttcatcaat accggcaagg cgctcacctc caccattgag
      121 tttcaaaaag ttcttcgcgt cattatggaa aaggcacaga agttgatcaa gtgcgaggcc
      181 tggtcgctct tcttgattga cgagaccacg caggagttat attttgggct ggtgaaggga
      241 aagatgaaca aaaaggtccg gcagtttcgg ctgaagatcg gtgaagggat tgcagggtgg
      301 gtggttaaga atgcaatgcc gatcatggtc ccggacgtcc ggaaagacca ccgtttttcc
      361 cgacacatag accgcgtcac cggttttaaa agccgttcga ttctgtgcgt tccgattgtc
      421 agcaagaaga agacgatcgg ggcgctcgag atgatcaaca aacatcatgt gatgttcgag
      481 aaaaaagatc tcgatctcct cacgaagctg gtcgatcagg cggccattgc actcgagcgg
      541 tccagtctct atcaaaagat ggctgacctt gcaatcacgg atgatctcac caagttgttc
      601 aacttccggt atctcgatca gaccctggac accgagctca agcgctccca acgctatggg
      661 gcgcagatct cgctgatctt ttttgacatg gactatttca agctcgtcaa cgatcggcac
      721 ggacatctca tgggaagcaa ggtgttaatt gaggtggcgc agatcctgat caacagcctt
      781 cgggatgtgg atatcgtgtc tcgatacggg ggggatgaat tcgtggtggt gctgcccgag
      841 acccgggtgg acaccgcaac caagatcgtg catcgtatcc acaaggcgat ccgcgagcat
      901 gagttcttaa aagaagaagg gctcaacctc catctctcgg cgagctatgg ggtctcgggt
      961 taccctgatc atgccaagac caagaaggac ctgatccgtc tcgcagacca ggcgatgtac
     1021 aaagccaaat cctctggccg cgaccgcatc tgtgttgtag gagaggagta aataatgctg
     1081 ttgcgatttt actccagcaa gtcctgctga ctctgct
//
LOCUS       DATMKH010000011         1802 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3858906, whole genome shotgun sequence.
ACCESSION   DATMKH010000011 DATMKH010000000
VERSION     DATMKH010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3858906"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="VNV63_00115"
     CDS             <1..656
                     /locus_tag="VNV63_00115"
                     /inference="COORDINATES: protein motif:HMM:NF012739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXC61050.1"
                     /translation="EFFVAGASEKRKVQIGDSVFPIQAFIQIPDTSQMLVDTQIREVD
                     IYKVKAGQEATIRVDAYPDLVLKGKVTLIGTLAEGREKEGSGGKYFNLQILLNSTDKR
                     LRPGMTARVEVLVDEEKDVLMVPVESVFQKGGRKMSYVVNNRGKIEEREIMTDKSNED
                     FVVVRNGLSEGERVLLLDPTKELSTFEKRSDSRSTTIPTATTENPKTKTEEEKKIVP"
     gene            761..1471
                     /locus_tag="VNV63_00120"
     CDS             761..1471
                     /locus_tag="VNV63_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXC61051.1"
                     /translation="MLIRMKDLSKVYSVGDGRLSVLKRINLEISAGEFIAIMGRSGSG
                     KSSLLHLLGCLDLPTSGTYELNGMLISALGDKELSSIRNKQIGFIFQTFNLIPQLNVL
                     ENVEVPIHYSGVVRRERRRQRFRFIKNVRFSEKRRPLQLIEKVGLLDRIQHTPPQLSG
                     GEMQRVAIARALVNDPPLLLADEPTGNLDTATAAGIMELVAELNRQGRTVIMVTHDQT
                     VAAYAKTVLYLQDGVLER"
     gene            1480..>1802
                     /locus_tag="VNV63_00125"
     CDS             1480..>1802
                     /locus_tag="VNV63_00125"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXC61052.1"
                     /translation="MLLWEVIRIGIRSLVSHKLRSLLSILGVVFGVAAVISMLSIGEG
                     ARLEALEQIRLMGTNNIIVRALPLSREKKLEAQQNLSMGLTASDAQRIKDISPLVDAV
                     SPIRES"
BASE COUNT          461 a          428 c          524 g          389 t
ORIGIN      
        1 ccgagttttt tgtcgccggg gcatccgaga agcgcaaggt gcagatcggt gactccgtct
       61 ttcccataca ggcatttata cagatcccgg acacctccca gatgctggtg gatacacaga
      121 ttcgggaagt ggatatctat aaggtcaaag ccggtcagga ggcgaccatt cgcgtggacg
      181 cctatccgga tcttgtcctg aagggaaagg tgaccctgat cgggacattg gccgaaggca
      241 gagagaagga ggggagcggg ggaaaatatt ttaatctcca gattctcctc aacagcacag
      301 ataaaaggct tcgccccggg atgacggcac gtgtggaggt tttggtggat gaggaaaagg
      361 atgtgctcat ggtcccggtg gagtctgtct ttcagaaagg cggcagaaag atgagttatg
      421 tcgtcaacaa ccggggaaag atcgaagaga gagagatcat gaccgacaag agcaacgaag
      481 attttgtggt ggtcagaaac ggattgtctg aaggggagag ggtgttgctc ctggatccga
      541 ctaaagaatt aagcaccttt gagaagcggt cggactcaag atccacgact atcccgaccg
      601 caacgactga aaatccgaag acaaagacag aggaggaaaa gaaaattgtc ccctaagcga
      661 ggagcaggct ttgcctgtcc gagcgcgggg tgtgggggca tcggaggacc tctctcagtt
      721 tcatccggac gggccctcac atacaaataa ggagcgaccg atgctgatcc ggatgaagga
      781 tttgagcaag gtctactccg tcggcgatgg gcgattgtcc gtccttaaac gtattaatct
      841 ggagattagc gccggcgagt ttatcgcaat catggggagg tcagggtccg gaaaatcttc
      901 cttgctgcat ctattgggct gcctggatct accaacctcc gggacctatg aactcaatgg
      961 catgcttatc agcgccctcg gcgataaaga gctctcatcc atccgaaata agcagatcgg
     1021 ttttatcttt caaaccttta acctcattcc ccagttaaac gttttagaaa atgtggaagt
     1081 tcctatccat tacagtgggg tggttcgaag agagaggcga agacagcgtt ttcgatttat
     1141 caaaaatgtc aggttctctg agaaaagacg accccttcag ttgatagaaa aggtcgggct
     1201 cttggatagg atccagcata cacctccaca gctctccggg ggcgagatgc agagggttgc
     1261 aatcgcaagg gccctggtca atgacccgcc gctgctcctc gctgacgagc ccaccgggaa
     1321 tctcgatacg gctactgcgg cgggaatcat ggagcttgtg gcagaactca atcgccaagg
     1381 gagaaccgtg atcatggtga ctcatgatca aaccgttgct gcctatgcga agacggtcct
     1441 ttatcttcag gatggcgtct tggaaagata aacgatcaca tgcttctctg ggaagttatc
     1501 aggatcggga tcagaagcct cgtttcgcac aagctgcgat ccctgctgag catcttggga
     1561 gtcgtcttcg gcgtggcagc ggtcatctcg atgctctcta tcggagaggg ggcgaggctg
     1621 gaggcactag agcagatccg gctcatgggt acgaataata tcattgtacg ggcgctgcct
     1681 cttagcagag agaaaaaatt ggaggcccag caaaacctct cgatgggtct cactgcaagc
     1741 gacgcccaac ggatcaagga catatcccct ctggtggatg ccgtctcacc gatcagggag
     1801 tc
//
LOCUS       DATMKH010000012         1013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2897731, whole genome shotgun sequence.
ACCESSION   DATMKH010000012 DATMKH010000000
VERSION     DATMKH010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2897731"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            199..>1013
                     /locus_tag="VNV63_00130"
     CDS             199..>1013
                     /locus_tag="VNV63_00130"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HXC61053.1"
                     /translation="MSAGFKILAYLIGTIVLGATLAPWLFWGGQHASHFKYLGFLATT
                     GFQRFFNRSVLISAVLLLIPLLRSIGLERLQNLGLRKNPRRVSHFLGGFLISACTIST
                     LGACLLWLNICELKTPLPWYQLPPLLLTAISVALIEEALFRGAILGLIRQTFPTVPAA
                     IFVAALFSIIHFLTPAKEQIVAVRWYSGFELLPHAFGRFLDPWMVLGGFTTIAVLGLL
                     LAHATIRTCSLWLGIGIHSGLIFGKMGFNKVTRKIHDLTPWFSGDITVGIGSVL"
BASE COUNT          186 a          291 c          236 g          300 t
ORIGIN      
        1 atcaacggct cgtcgtaata ggtccagcga tcttcgtctg cgcttttgga ttggaagcgc
       61 cacgaaccac ccgtcttgat cacccgaact tcccgtttta atccctcatc ggtgcgaatg
      121 atccaggcgt gcctattgtg catgacagag aaactttagt ttgctgcgga tcatcgtcca
      181 cgctaattcc ttctcctctt gagcgctggc tttaagattc tcgcctatct gatcggaacc
      241 atcgtgcttg gagcaacgct cgccccttgg cttttttggg gaggccagca tgcctcccat
      301 ttcaagtacc tggggtttct cgccaccaca ggatttcagc gcttctttaa ccgttccgta
      361 ttaatttccg ccgttctgct tctaatccca cttctacggt cgatcggact ggagcgactc
      421 cagaatcttg gcctgcgcaa aaatccccgg cgagtatccc atttcctcgg cggttttttg
      481 atctccgctt gcaccatttc cactttgggc gcctgtcttc tatggctcaa tatctgtgaa
      541 ttgaagactc cgcttccatg gtatcagttg cctcccttac ttctgaccgc catctccgtt
      601 gccctcatcg aggaagcctt gttcagagga gcaattctcg gtttgattcg acagaccttt
      661 cccacggtgc ccgcggccat ttttgttgcc gcactttttt ccatcataca ttttctgact
      721 ccagccaaag aacagatcgt cgcggtacgc tggtattccg gtttcgaact gttgccccat
      781 gcgttcggaa ggtttttgga cccatggatg gtcctgggtg ggttcacgac gatcgccgta
      841 ctcgggttat tgctggcgca cgcgacgatt cgcacctgct ccctctggct cgggattggc
      901 attcattccg ggttgatttt cggtaagatg ggatttaaca aagtcacaag aaaaattcat
      961 gatcttactc catggttcag cggcgatatt accgtcggca tcggatctgt gct
//
LOCUS       DATMKH010000013         1034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2576551, whole genome shotgun sequence.
ACCESSION   DATMKH010000013 DATMKH010000000
VERSION     DATMKH010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2576551"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..>1034)
                     /locus_tag="VNV63_00135"
     CDS             complement(135..>1034)
                     /locus_tag="VNV63_00135"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013579398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HXC61054.1"
                     /translation="NANGARNPFYEFMREVVPGDLVFSFVDTRIAAIGTVTSYCYESP
                     KAAEFGGVGLNWEAIGWRVRVNFVSLLNKIRPKDHIDVLRRVLPSRYSPLQDSGNGIQ
                     SVYLTEVPMPFAEVLIGLIGKEAAQVNDRAARTTLQTPMQVENADLEIWEHHIEAKIE
                     SDRQIPETEREALIVARRGQGLFKQRVMQVETRCRITGVTNPIHLRASHCKPWRDSSN
                     EERLDGENGLLLTPTMDHLFDRGFIGFEDSGILIVSPVAHAPSLDRMGVATDRIINVG
                     TFTQGQKTFLDYHRESVLLQARH"
BASE COUNT          226 a          283 c          271 g          254 t
ORIGIN      
        1 atcttctcga ttggccttga ttgatttcgt taggtgccca ctttaatgcc ttgtgaccag
       61 gaaaacggag gcagggaagc gttcatcttt cagttccgaa ctcggcctgg ggatgtgact
      121 actgtgtagc ccgcctagtg gcgagcttgc aagagaaccg actctcgatg gtagtcaagg
      181 aaagtttttt ggccctgggt gaatgttccc acgttgatga ttcggtccgt tgctactccc
      241 atccggtcca aagaaggagc atgggcaact ggcgagacga tcagtattcc ggagtcttcg
      301 aaaccgatga aaccgcgatc aaagaggtgg tccattgtcg gagtcaatag aagcccattt
      361 tcgccgtcga gccgctcctc gttgcttgaa tcccgccagg gcttgcaatg actggctcgt
      421 aaatgaatcg gattcgtaac gcccgtgatt ctgcatcggg tttcaacttg catgacccgc
      481 tgcttgaaca gaccctgtcc ccgtcgcgcc acaatgagtg cttcacgttc ggtctccgga
      541 atttgacgat cagattcaat cttggcctcg atgtgatgct cccaaatttc cagatcggca
      601 ttttcgacct gcatgggtgt ctgcaacgtc gttctggcgg cccggtcatt tacttgtgcc
      661 gcctccttgc caatcaagcc gatgagtacc tccgcaaaag gcatcggtac ctcggtcaaa
      721 tagaccgatt ggattccgtt cccggagtcc tgcaaagggg aatagcgtga aggcaacaca
      781 cgcctcagca catcgatgtg gtccttgggg cggatcttgt tcagaaggga gacgaaattg
      841 acccgtaccc gccatccaat tgcctcccaa ttcaggccga ctcctccgaa ttcagcggcc
      901 ttcggactct catagcaata ggacgtgact gtcccgatcg cggcgatccg ggtgtccacg
      961 aaagagaaaa caaggtctcc gggtactacc tctctcatga attcataaaa tgggttccga
     1021 gctccgttgg catt
//
LOCUS       DATMKH010000014         1612 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3859893, whole genome shotgun sequence.
ACCESSION   DATMKH010000014 DATMKH010000000
VERSION     DATMKH010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3859893"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VNV63_00140"
     CDS             <1..243
                     /locus_tag="VNV63_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61055.1"
                     /translation="LKLSNHFDPNDPNSRDDFTILITGAIKAANLNPTAVLTDFAKTL
                     EEKLPKSANVEISKNAVIQGAEAFASFDLRIRYVSQ"
     gene            276..761
                     /locus_tag="VNV63_00145"
     CDS             276..761
                     /locus_tag="VNV63_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61056.1"
                     /translation="MVGLTIVALEAIFLAANFDHFQSQIVQNSKMTFSLTQREAALQR
                     FKLKKDPLENKANLLLSGYTPEPLAIGPETFGDLFSLGVQIAPQEVSANKGVKIWTYR
                     LSSTTIEYHRLMPAISALENQYPLGRFIEIDLKSKGLPFALTPGPIDFAGIFSTLRGR
                     P"
     gene            758..1321
                     /locus_tag="VNV63_00150"
     CDS             758..1321
                     /locus_tag="VNV63_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61057.1"
                     /translation="MRTIIAAAPSVLTLLLCAGLSYGQDNSPQPPNQATDPKVYPREV
                     TDLPNFTRNDYVRSPRDPFLDKSVKSTIMGEYKANVLDKLVLVSWLDDAGKIVIFVQN
                     TETNDVQEVTSEPNKDHFRIVEIHPNADPKLFEAVISNGSEKGPVRFRLTPPDRNPQI
                     GPTSPPATPTKKPQIGTASPSPGQRKN"
     gene            complement(1391..>1612)
                     /locus_tag="VNV63_00155"
     CDS             complement(1391..>1612)
                     /locus_tag="VNV63_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61058.1"
                     /translation="PGSRSYLPTSRNGVQITDINSLLKAFELTDNGTPTGNIVATIAV
                     GPMLDLTSGMRPMLPIIDWRSGTPQEIQF"
BASE COUNT          406 a          451 c          393 g          362 t
ORIGIN      
        1 ctaaagctct ccaaccattt cgatcccaat gaccctaaca gcagagacga tttcacaatc
       61 ctgatcaccg gcgcaatcaa ggccgcgaac ctgaacccga cggccgtcct tacggatttt
      121 gcgaaaactt tggaggaaaa gttgcccaag agcgcgaacg tcgaaatttc taaaaacgca
      181 gtgattcaag gggcggaagc cttcgcttcg ttcgatctca gaattcgtta cgtcagccaa
      241 tgagaccttc acttaccgca ttcttgagct ttttggtggt cggactgaca attgtcgctc
      301 tggaggctat cttcctggct gccaattttg atcatttcca gagccagatc gtgcaaaaca
      361 gcaaaatgac cttcagcctt acgcagcggg aggcggcgct gcaaaggttc aagctaaaga
      421 aggacccatt ggaaaacaaa gcaaaccttc tgttaagcgg ctataccccg gaaccgctgg
      481 caatcggacc ggagactttc ggagatcttt tttcccttgg ggttcaaatt gcaccacagg
      541 aggtatcagc gaataagggt gtgaagattt ggacttacag actgagtagc accacgatcg
      601 agtaccatcg cctgatgccg gcgatttctg cgctggaaaa ccaatacccg ctcggtcgat
      661 ttatcgagat cgacctgaaa tctaaaggcc tgccttttgc gctgacccct ggtccaatcg
      721 atttcgccgg aattttttca accttgcgag gacgcccatg aggacgatca ttgctgccgc
      781 gccctctgtt ctgactctcc tcttatgcgc cggtttgagc tacggccagg ataattcgcc
      841 ccaacctccc aaccaggcga cggatccaaa ggtgtacccg cgagaggtta cggatctgcc
      901 gaactttact cggaatgatt acgttcggtc tcctcgcgac ccgtttctgg acaaatcagt
      961 caaaagcaca atcatgggtg agtacaaagc caacgttctt gataagctgg tgttggtcag
     1021 ttggcttgat gacgccggaa agatcgtcat ctttgtccaa aatacggaga ccaatgacgt
     1081 gcaggaggtc acctccgaac ccaacaaaga ccatttccgg atcgtggaga ttcaccccaa
     1141 cgcagatccg aaattgttcg aagcggtgat ctctaacgga agcgaaaagg gaccggtgag
     1201 attccgtcta actccgccgg atcggaaccc gcagatcggt cctacgagtc ccccggcgac
     1261 accaacaaag aagccgcaga tcgggaccgc gagtccatcg cccggccaga gaaaaaactg
     1321 agaacctctc tcgtaagtaa ggcgtcccgc cttaccctct tccgcaatcc aagcgtcccg
     1381 ccttactcac tcagaactga atttcttgag gcgtaccgct tctccaatct attatcggca
     1441 gcattgggcg catcccactc gtaagatcca gcatcggtcc aaccgctatg gtagcaacga
     1501 tattcccggt cggcgtacca ttatccgtaa gttcaaaggc tttcagcaag gagttaatat
     1561 ctgtgatttg gacgccgttg cgggatgtcg ggagatacga tctgctaccc gg
//
LOCUS       DATMKH010000015         1068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_653298,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000015 DATMKH010000000
VERSION     DATMKH010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653298"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..948)
                     /locus_tag="VNV63_00160"
     CDS             complement(136..948)
                     /locus_tag="VNV63_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009478438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HXC61059.1"
                     /translation="MTTKGKVLVLVSSGHGLPLKDGKVYTGAGYYLNELTVPVRALMK
                     EGYEITFANPKGNTPQMDVHSAVADFFGGDEARLQDYLKFRDTLTGLKNPTPISEVIA
                     SGLDQYDAVFVPGGHGPMMDLLDDPDAGTVMRHFHKTSKPTAVLCHGPISLLSALPNS
                     KEVVAALSAGDAAGAHAKAQGWIYSGYKMTIFSTAEEQQREPLEIGGKVLFYPDFALR
                     TAGGDVSVAAPWQSYVLQDRELISGQNPFSDEALLKLLLPALSEKKKVVSAA"
BASE COUNT          229 a          308 c          283 g          248 t
ORIGIN      
        1 ttggatcgag tcttcttgac tttcacaacg cagcgaaagg agaaacaatt ggaccaaggt
       61 atcggggaac tcccgtctgt ccccagtttt tcccgtcccc agttttcccc gaattttccc
      121 gggcgtctcc gactctcatg ccgctgaaac caccttcttc ttctcactga gtgccggtag
      181 aagaagtttc agaagcgctt catcggagaa cgggttctga cctgagatca gctcgcgatc
      241 ttgaaggacg tagctctgcc atggagcggc cacactcacg tcaccgcccg ccgtacgcaa
      301 ggcgaagtcg gggtagaaga gaaccttacc gccaatctca agcggctccc gctgctgctc
      361 ttccgcggtc gaaaagatgg tcattttata tccagaatag atccaacctt gagcctttgc
      421 gtgcgctcca gcggcatcgc ccgcactcag agccgcgact acctccttcg aattcggaag
      481 agccgagaga agtgaaatcg ggccatggca gagcactgcc gtaggcttgg atgttttgtg
      541 aaagtgtctc atcacggtgc cagcgtcggg atcatcgaga agatccatca tcgggccgtg
      601 gccgccgggg acgaaaacag cgtcatattg atccaggccc gatgcgatga cttccgagat
      661 aggagttgga tttttgagcc cggtcagggt gtcgcggaat ttcagatagt cctgaagcct
      721 agcctcgtcc ccgccgaaga aatcggcgac agctgaatgg acatccattt ggggcgtgtt
      781 gcctttcggg ttggcaaaag tgatttcgta gccctccttc atcagggcgc gaaccggaac
      841 cgtcagctcg ttgagatagt agcctgcgcc ggtataaact ttgccatctt tcaagggcag
      901 gccgtgaccg ctcgagacca gtactaagac ttttcctttt gttgtcattt tgttctcctc
      961 tctgaaatcg aatctgtttc ccgagatctt aacttgtgcg gagcaaccca atccgcgaga
     1021 gcctcggcct cagccacggt ccccattgcg tccacaagca ccgcgtcg
//
LOCUS       DATMKH010000016         1448 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_12063,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000016 DATMKH010000000
VERSION     DATMKH010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1448
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12063"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="VNV63_00165"
     CDS             <1..284
                     /locus_tag="VNV63_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013756715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HXC61060.1"
                     /translation="SKVFGLLDISPEKAQERFGFLLKALTYGAPPHGGMAVGLDRIAM
                     LLTGASNIREVIAFPKTAKAVDLMTGSPSEIDEELLKELKIKIELEDLT"
     gene            368..1306
                     /locus_tag="VNV63_00170"
     CDS             368..1306
                     /locus_tag="VNV63_00170"
                     /inference="COORDINATES: protein motif:HMM:NF033581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HXC61061.1"
                     /translation="MQISFGMYEDKNRRLKKTMLEEVDKVLDFAKIEKLLMEMYRGTT
                     GRPPIPPLILFKCLLLESWYGLSDVEVVQEIHDRRSFERFVGEGVREYHVDDTTLVKF
                     RERLREAGLIDRVWAAVQQGLDRKGLLVKKGAIVDSTLVEGACKPESKDQDGKPVDED
                     VHYTSRQGKAVDGMKVHVGQDEKSGLIRKMDLSHIEEHDHEHFKDMVPHGVEEAYADK
                     AYRSAEHDEYLKGQGIRNRVLNRVWRGQNLTPRQIKQNKVWGRVRCRIEATMSTLKRW
                     CSMGRMRYYGMARNKIWVLICGIAFNGKRAVKLAVA"
BASE COUNT          391 a          314 c          442 g          301 t
ORIGIN      
        1 aatccaaagt tttcggcttg ctcgatatct cgccggaaaa ggcccaggag cgttttggct
       61 tcctgctcaa ggccttaacc tatggcgccc cgccccatgg tggtatggcc gttgggctgg
      121 accgcatcgc catgcttttg acgggcgcct ccaacatccg ggaagtcatc gctttcccga
      181 agaccgccaa ggctgtcgac ctgatgaccg gttcaccatc ggaaatagac gaggaattgt
      241 tgaaggaatt gaagatcaag attgaactag aggatttaac ctaagtttaa atagactgta
      301 acctctgaaa aactccatcg tctaaatgct tgaaatataa taacagcatt ccagacggag
      361 gaaacctatg caaatcagct tcgggatgta cgaggacaag aaccggcgtc tcaaaaagac
      421 catgctggag gaagtggata aggttttgga ctttgcgaag atcgagaaat tgttgatgga
      481 aatgtaccga ggcaccacgg gacgccctcc gatcccacct ttgatcttgt ttaagtgtct
      541 tttgctggaa tcctggtacg gcctatcgga tgtagaggtg gttcaggaaa tccacgaccg
      601 tcgcagtttc gagcggttcg tgggagaagg ggtgcgggag tatcacgtcg acgacaccac
      661 cttggtgaag tttcgggaaa gactccggga agccggattg atagatcggg tttgggccgc
      721 agtgcagcag ggcttggacc gcaaaggcct tttggtcaag aagggtgcca ttgtggactc
      781 caccctggtg gagggagcct gcaagccgga atccaaggat caggacggaa agccggtaga
      841 cgaggatgtt cattacacct cccggcaagg caaagcagtg gatgggatga aggttcatgt
      901 gggccaagac gagaaaagcg gcctgattcg gaagatggat ttgagccata tcgaggaaca
      961 cgaccacgaa catttcaagg acatggttcc ccatggggtg gaagaggcat acgccgacaa
     1021 ggcctatcgg tcagcggaac atgatgaata tttgaaggga caagggattc gcaatcgtgt
     1081 gttgaatcgg gtttggcggg gacagaacct gaccccaaga cagataaagc aaaacaaggt
     1141 ttggggccgt gtgaggtgcc ggatcgaggc gacgatgagt accttgaagc ggtggtgttc
     1201 gatggggcga atgaggtact acgggatggc ccgcaacaag atttgggttt tgatctgcgg
     1261 tatcgctttt aacgggaaaa gggcggtaaa gctggcggta gcttgagcta agaggagttt
     1321 ttcggaggca acggacgccg aaagggccga aaagggagtt caatagcgaa gtgcaccacc
     1381 cctggagaga caaaaaaatc tcttggaagt agcgaaaacc ctgataagcg gagtttcaaa
     1441 ctcaaaca
//
LOCUS       DATMKH010000017         1209 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1294800, whole genome shotgun sequence.
ACCESSION   DATMKH010000017 DATMKH010000000
VERSION     DATMKH010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1209
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1294800"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..809
                     /locus_tag="VNV63_00175"
     CDS             <1..809
                     /locus_tag="VNV63_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013042819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXC61062.1"
                     /translation="LQLAKKKKDFISDTVGRTSKALGLDHMLERKPHALSGGQRQRVA
                     LGRAIVRSPKVFLFDEPLSNLDAKMRVQMRSEISKLHAQLGSTMIYVTHDQVEAMTMG
                     DRICVMRDGLIMQVADPLTLYRQPENLFVAGFIGSPPMNLLKGKIEKREGGLFFVETA
                     EKNGLTFPLKGHLEALASKYVDKDVVFGIRPEHLSDEIKDGLAAPITSTVEIAEPMGS
                     ESLVYLKAGTGNLIARIHGEHLYHLGEKVTVQLNMDKVNLFDSATENVIR"
     gene            818..1051
                     /locus_tag="VNV63_00180"
     CDS             818..1051
                     /locus_tag="VNV63_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61063.1"
                     /translation="MRQILDTEQYVQVPPMMLSDPFYRITYLIKEEIRKYKWIEGENG
                     RQLTWEQARHDWMKKHREKYEKFVLETLSFPEE"
BASE COUNT          314 a          302 c          321 g          272 t
ORIGIN      
        1 gcctccaatt ggcgaagaag aagaaagact tcatctccga caccgtaggt cgaacctcaa
       61 aagctcttgg actcgaccat atgttggagc gcaaaccaca cgccttgtcg ggcggtcagc
      121 ggcaacgcgt ggctcttggg cgcgccattg tgcggagccc aaaagtcttt ctcttcgacg
      181 aaccgctgtc gaacctggac gccaagatgc gcgtccagat gcggtcagaa atctcaaaac
      241 tccacgccca actcggttcc acgatgattt acgtcactca tgaccaagtc gaagcgatga
      301 ccatgggaga ccggatctgc gtgatgcgcg atggcctgat tatgcaggtt gcggatccac
      361 tgaccctcta ccggcagccg gaaaatcttt tcgtcgctgg ttttatcggc agtcccccga
      421 tgaaccttct gaaagggaag atcgagaaac gtgagggtgg tttgttcttc gtggagactg
      481 cggaaaagaa cggtctcacc ttcccgttga aaggtcactt ggaagccctg gccagcaagt
      541 acgtcgataa ggatgtcgtt tttgggattc gacctgaaca cttgagtgat gagatcaaag
      601 acgggctcgc ggccccaatc acttcgaccg ttgaaatcgc tgagccgatg ggatcagagt
      661 cactggtcta tctgaaagcc ggcaccggaa atctgatcgc ccgaattcat ggggagcatc
      721 tctatcatct gggcgaaaag gtgaccgttc agttgaatat ggataaggtc aatttatttg
      781 attcggcgac cgaaaacgtc attcgatagc accgcccatg cgtcagattc ttgataccga
      841 acaatatgtt caggttccgc cgatgatgct ctcggatcca ttttaccgga tcacttacct
      901 gatcaaggaa gagattcgaa aatacaaatg gatcgaaggg gaaaacggtc gccaattgac
      961 ctgggagcaa gcacggcatg attggatgaa aaagcatcgg gagaaatacg aaaaattcgt
     1021 attggagacg ctttccttcc ccgaagagta gctctttcct ttcagcttcg gttcaagaac
     1081 gagacgatcg aaaccgctac ccggccagca gggcagcctg atgcgaatag cattaaaggg
     1141 caaaaaggta agaacgtatt gaacctgttc ttactttttg tccgcgcagg cttcgtgtct
     1201 tgggcaaga
//
LOCUS       DATMKH010000018         1941 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4502680, whole genome shotgun sequence.
ACCESSION   DATMKH010000018 DATMKH010000000
VERSION     DATMKH010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4502680"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..>1941
                     /locus_tag="VNV63_00185"
     CDS             86..>1941
                     /locus_tag="VNV63_00185"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXC61064.1"
                     /translation="MIALELVLYGVRHFLDMKKLTFGPGFNLVHGRNGAGKTTIADTF
                     FEILSWEKDRPTLQSLAPLERKDLCQAALIFQSQDGGIYRLVRDFLKDIGSLSKLDQN
                     NKFNLLSKDEDQITGFIHSEIQKLSPGQLRDELLLSRAGMPSSSHFTSHAAGPASQTG
                     NQSKTSDLASSPLKAKRLEEIRASLVLADQIAKFEEQLDKVQNRLLELRRQHERYEND
                     TQELKQLLQEQAAYEGFQDLPKNVSSLLDEYKITEKTHTQEVRELDEQRISIEDQLTV
                     IPATPIFLSKLFLAGIGVMVLSLLLALFLQLGGVWQTLYFLGLLTGAVLVVVSVFLDV
                     RRLTHRKSIEGKLGEISHAQEISESRFKKQNTAVFDLLKKASCQNVDQLKDKIRSYLE
                     LARSIQNLKEEQSRLLGNKSLPDLEREQQALTVSAKELESKIKGVQGTTPDIYSLQEE
                     MRTIQREIQEQKTLKPKEAVKAEQVPDQTVTEERSSSVTSFRRPKQFETVPKKLFETH
                     ATEWLRRLTQGRYDRLEYDPQSNLTLIDRKGSPALFDQLSSGVQDQVYLSILWAGWQL
                     TSSGAPALPLVLDDPLVGLDEASRQVAIETLKELGQHRQIILLTTASHPNKD"
BASE COUNT          530 a          513 c          493 g          405 t
ORIGIN      
        1 gtatcggaag tctcccgcat ccaaacagtc tacaaaaacg catggcagcc cgctttgagc
       61 aggagaggat ggttcggatc ccatcatgat cgctctggaa ctcgttctct acggtgtccg
      121 acatttccta gacatgaaga agctcacctt tggtcccggc ttcaatctcg tccacggtag
      181 aaacggagcc ggtaaaacca cgatcgcgga caccttcttt gagatactgt cctgggaaaa
      241 agacaggccg acacttcaga gcctcgcacc gctcgagcgg aaagacctct gccaggctgc
      301 cttgatcttt cagagccagg acggcggcat ctatcggctg gtccgggatt ttttgaagga
      361 tatcggcagc ctctccaagc tggatcagaa caataaattt aatttgctca gcaaagatga
      421 agaccagatc accggcttca ttcattcaga gattcaaaaa ctatccccgg ggcaactgag
      481 agacgagttg ctcctgtccc gagccggaat gccgtcttct tcccatttca cgtcgcacgc
      541 cgcaggcccc gcgtcccaaa cagggaacca aagcaaaaca tcggacctag cctcttcgcc
      601 gcttaaggca aaacgtctcg aagaaatccg ggcttccctg gtcctggcgg accagatcgc
      661 caagttcgaa gaacaactgg ataaggttca aaaccgtctc ttagaactca ggagacaaca
      721 tgagagatac gagaatgaca cacaagaatt aaagcaactc cttcaggaac aagcggccta
      781 cgaaggattt caagacctgc ctaagaacgt ttcgagcctc ctggacgaat acaaaattac
      841 cgagaagacg catactcagg aagtccgcga actcgatgaa caaagaatct ctatcgaaga
      901 ccaactgaca gtcattccgg ccacaccgat tttcttgagt aagctttttc ttgccgggat
      961 cggcgtaatg gttctctcgc ttctcctggc gctcttcctc caactgggag gggtttggca
     1021 gacactctat ttcttggggt tgctgaccgg ggccgtgctg gtggtggtct cggttttcct
     1081 ggatgtccgg cggcttacgc accgaaaatc cattgagggc aagctgggag agatatccca
     1141 tgcacaagag atatcggagt ccaggttcaa aaagcaaaac accgcggtct tcgatttatt
     1201 aaagaaggcc tcttgtcaaa acgtggatca gcttaaagac aaaatcagaa gttatctgga
     1261 actggctcga tccatccaga atttgaaaga agaacagagc aggctcttgg gcaataaaag
     1321 cctgcctgac ctggaacgcg agcagcaagc tttgacggtt tccgccaagg aactggaatc
     1381 aaaaatcaag ggggttcaag ggacgacgcc cgatatctat agcctccagg aagagatgcg
     1441 gacgattcag cgcgagatac aggagcaaaa gacattgaaa cccaaagagg cagtcaaggc
     1501 ggaacaagtt cccgatcaaa ccgtaacgga ggagagaagt tcatcggtca cgtcgtttcg
     1561 acggccaaaa cagttcgaga cggtgccgaa aaagctgttt gaaactcatg caaccgaatg
     1621 gctccggcgt cttacacagg gccggtacga tcgtctcgaa tatgatccac aatcgaatct
     1681 cactctgatc gatagaaaag gatcaccggc gctttttgac cagttgagtt ccggagttca
     1741 ggatcaggtc tatctttcga tcctgtgggc cggttggcag ctgaccagct caggcgcccc
     1801 tgctttgccg ctcgtgctgg atgatcctct cgtgggactg gatgaggcca gccggcaggt
     1861 tgcgatagag actttaaaag aactgggcca gcaccgccaa atcattctgc tgaccacggc
     1921 atcccatccg aacaaagaca a
//
LOCUS       DATMKH010000019         3006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3861627, whole genome shotgun sequence.
ACCESSION   DATMKH010000019 DATMKH010000000
VERSION     DATMKH010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3006
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3861627"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2283)
                     /gene="recG"
                     /locus_tag="VNV63_00190"
     CDS             complement(<1..2283)
                     /gene="recG"
                     /locus_tag="VNV63_00190"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HXC61065.1"
                     /translation="MPNLIGILEIMRKPIEYATREEFAYFKKVQDIDSFLNDQIEAAL
                     KSTKAASERYEIRRLQTLLKNYQQFSEQDRKQCLLCVKVILEGLIQKVTRTSENVSRP
                     MSHVSGPTSERVTCNVQHDPQIPRHTSEVTRPKLEHITCSVQRDPFEWTSPVQYIKGV
                     GPQRARLLEKVGIRTVEDALYFLPRRYEDRGNLKPISQLISGESQTICGEVMAVDLVV
                     TPRRGMRILELALQDKTGTVYAKWFNQPYLKNIFKKGQRLVLSGKSTTNRYYGHALEM
                     ENPNYEILDAEDQELLHTGRIVPIYPETKGLSSRPIRLLTDRLFELYSPVIPEILPEA
                     IRKEYGLLPLSQALQMVHFPLPGSDLARFNRGNSDAHRRLIFDEFFLLELGLALKKRG
                     VQEEEKGIHFEIGTSKVKRFIEQLPFQLTTAQRRVMDEIQRDMASVHPMNRLIQGDVG
                     SGKTVVALFAMFIAVENNYQAVLMVPTEILAEQHYLTLGPLLDRLGISCALLTSGHSR
                     EEKKRISEAAETGSASILIGTHALLQEGVRFQRLGLAVIDEQHKFGVLQRATLTRKGY
                     HPDVLILTATPIPRTLALSVYGDLDLSILDEMPPGRKPVITKLFNESQRQTVYSLIEK
                     ELSGGHQVYVVYPLVEESEKVDLKAAVKMAGQLEEMYPRYHVGLLHGKMKSQEKEQVM
                     RDLSRGKIQLLVSTTVIEVGIDVSNATVMLIEHAERFGLAQLHQLRGRVGRGAAQAYC
                     YLLASFPRSEDAKRRLGAMVK"
     gene            2391..2582
                     /gene="rpmB"
                     /locus_tag="VNV63_00195"
     CDS             2391..2582
                     /gene="rpmB"
                     /locus_tag="VNV63_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HXC61066.1"
                     /translation="MARTCEICGKRRMVGNNVSHANNRTKRAFNPNIQTVRAVIQGNH
                     KRIKVCTRCLRSGWVSKIA"
     gene            complement(2616..>3006)
                     /locus_tag="VNV63_00200"
     CDS             complement(2616..>3006)
                     /locus_tag="VNV63_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61067.1"
                     /translation="KDPRLNQGCTSESDVGFVGLATKEFASVTVHLNLGYFFIGSPSG
                     IDLKNVFKYSLAIQDHSILPPAITPMIELAGETQRRPSINSDPISVLVGMGYDVGQGI
                     LLDAGFGIGVTRASPDYSATVGMTYHF"
BASE COUNT          750 a          798 c          717 g          741 t
ORIGIN      
        1 tttcaccatc gcacccaacc gccgtttggc atcttcactc cttgggaaag aagccagaag
       61 atagcaatag gcctgggccg ctcccctccc cacccttccg cgaagctggt ggagctgagc
      121 cagtccaaat cgttccgcat gctcgattag catgactgtg gcattcgaaa catcaatccc
      181 gacctcgatc acggtagtgg ataccaagag ttggatcttt ccccggctta aatcacgcat
      241 cacctgttct ttttcctggg atttcatctt gccatggagc aatccgacat gataacgcgg
      301 gtacatctct tcaagctgcc cggccatctt cacggccgct tttaaatcca ccttctcgga
      361 ctcttcgacc aaaggataga ccacatacac ctgatggcct ccggacagtt ctttctcgat
      421 caaactataa accgtttgac gttgtgattc attaaaaagt tttgtgatga cgggtttccg
      481 acccggaggc atctcgtcca gaatggacag gtctagatcc ccatacacgc ttaaggccaa
      541 ggtccgagga atgggagtag cggtcaagat caggacatcc gggtgatagc ctttccgcgt
      601 caatgtggcc cgttgcaaaa cgccgaattt gtgctgttca tcaatgaccg ccaggccgag
      661 acgctgaaag cggacaccct cttgaagcaa cgcatgagtc ccgatcagta tagaggccga
      721 tcctgtctcc gccgcttcac tgatcctctt tttttcttcc cgcgaatgtc cgctggtcag
      781 caaggcgcag gaaattccca gcctgtctaa aagaggccct aaggtgagat aatgctgctc
      841 cgccaagatc tcggtcggaa ccattaagac cgcctgatag ttgttttcca ccgcaatgaa
      901 catcgcaaac agggcgacaa ccgtcttacc gctcccgaca tccccttgaa tgagccgatt
      961 catcggatga acactggcca tgtccctctg gatttcatcc atgacccgtc tctgagccgt
     1021 ggttagctga aacggcagct gctcaataaa acgttttacc tttgatgtgc cgatctcaaa
     1081 atgaatcccc ttttcttcct cctgaactcc cctttttttg agcgccagac ccaactcgag
     1141 aaggaaaaat tcatcgaaga taaggcggcg atgcgcatcg ctgtttcccc ggttgaatcg
     1201 agcaagatcg cttcccggaa gagggaaatg gaccatttgg agcgcctggg ataacggtaa
     1261 gagcccgtat tcctttcgaa tcgcctccgg aagaatttca ggaataaccg gcgaatacag
     1321 ttcaaagagc cgatcagtta ataatcggat cggccgggag ctgagacctt tcgtctcggg
     1381 ataaattggc acgatccgcc cggtatggag aagttcctga tcctcagcat ccagaatctc
     1441 gtaattggga ttttccattt ccaaggcatg gccgtaataa cggtttgtcg tgctctttcc
     1501 gctcaagact agccgctggc cttttttaaa gatatttttt agataaggct gattaaacca
     1561 tttagcatat acggttcccg tcttgtcctg caaagcaagc tccagaatac gcatgccgcg
     1621 acgcggagtg acgacgaggt ccaccgccat gacctctccg cagatggtct ggctctcgcc
     1681 gcttatcagt tgtgagatag gttttaggtt ccctcgatcc tcatatcttc gtggaagaaa
     1741 atataaggca tcctccacgg tcctgatacc gactttctcc agaagcctgg cgcgttgagg
     1801 tcctacacct ttgatatact gaaccggcga ggtccattca aaaggatcac gttgcacgct
     1861 acaggttata tgttcaagct tggggcgtgt gacttcggac gtgtggcgtg gaatttgagg
     1921 atcatgctgc acattgcacg ttacacgttc agacgtgggg cctgagacgt gcgacatggg
     1981 gcgtgaaacg ttttctgaag ttctggttac tttctgaatc agaccctcca gtatgacctt
     2041 tacgcacaga aggcattgct ttcggtcttg ctctgaaaat tgctgataat tcttcaagag
     2101 ggtttgtagc cgccgtatct catatcgttc tgaggccgcc tttgttgact tgagagcagc
     2161 ctcaatttgg tcgtttaaga atgagtcgat gtcttggact tttttaaaat acgcaaactc
     2221 ctcccgcgtc gcgtactcga tcggcttccg catgatctcg agtatcccta tcaaatttgg
     2281 catttgaatt cagcttgtca aaagcctggc ggtatgttac catagcaaac ttcatgaagg
     2341 caagaaagag aaactaaaac ataaagcttt acgctagatg aggagacgtg atggcaagga
     2401 catgtgaaat ctgtggtaaa cgccgaatgg tcggaaataa tgtcagtcac gcaaacaacc
     2461 ggacaaaacg ggcgttcaac ccaaacattc aaaccgtgag agccgtgatc caaggcaatc
     2521 acaagcggat caaggtttgt acacgctgcc ttcggtccgg atgggtatcg aaaatcgcct
     2581 aaaacagatt tgacggcttt taagtggcgg cacggctaaa aatgatatgt cattccaacc
     2641 gtggcactgt agtccgggct cgcacgtgtg acgccaatgc caaacccggc atctaggaga
     2701 ataccctgcc cgacatcata tcccattcca actaacacag agatcggatc cgagttgata
     2761 gagggtctgc gttgagtctc tcccgccagc tcgatcatcg gggtgattgc aggtggcaaa
     2821 atggagtggt cctgaatcgc caggctgtac ttgaacacat tcttcaggtc tatcccggac
     2881 ggactcccaa tgaaaaaata acccaagtta agatgaactg taaccgaagc gaactcctta
     2941 gtcgccaggc ccacaaaacc cacatccgat tcactcgtac agccttgatt aagacggggg
     3001 tccttc
//
LOCUS       DATMKH010000020         1164 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2258365, whole genome shotgun sequence.
ACCESSION   DATMKH010000020 DATMKH010000000
VERSION     DATMKH010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1164
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2258365"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1164)
                     /locus_tag="VNV63_00205"
     CDS             complement(<1..>1164)
                     /locus_tag="VNV63_00205"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HXC61068.1"
                     /translation="LAAGYFQGRITRRRFIQQATKLGISAALLSRIAPSAFAADANLL
                     DSSPEVPDEAPITKERVEFLKSKPYKGTTINVMVLKATVGDGLKYHAPRWEKQTGGKI
                     NVAEVPIDTLHQQIFSDLSTGLGRYDAYMTGAWFYGDFFTGKEPYIVEIEPFLKDPKT
                     PYWDPNDFLPAMKKLYTWQGKLYGVLFDADAQILYYRRDILSNPDHGKKFKDKLGYDL
                     PNPPKSTKEMHDVATFFTGWDWNGDGQNDWGISLHAKVNEQGFFHFLTLSAPYVISPN
                     NKHYFFDPDTMKPLINSEGHLRALEEYSKFLANGPKEQISWTLGQGWNLFLAGHAVME
                     PTWGDLPTFAQDEKMSKVKGKIGATVIPGTQEAFNPITGKWEKFELNVAGNTNG"
BASE COUNT          262 a          303 c          300 g          299 t
ORIGIN      
        1 ccgttggtgt tcccagcaac gttcagctca aacttctccc atttgccggt gattgggttg
       61 aatgcctctt gagtgccggg gattaccgtc gcgccgatct tccctttcac tttggacatt
      121 ttctcgtcct gggcgaatgt cggcagatca ccccacgtgg gttccatgac cgcgtgcccg
      181 gcaaggaaca aattccatcc ctgcccgagc gtccagctga tctgctcttt gggaccattg
      241 gccaagaatt tcgaatattc ttccagcgcc cggagatgac cctcggagtt aattagaggc
      301 ttcattgtat ccggatcgaa gaagtaatgt ttgttgttcg gggaaatgac gtagggcgcg
      361 gaaagagtga ggaagtggaa gaatccctgt tcattcactt ttgcatgaag cgagattccc
      421 cagtcatttt gtccatcacc attccaatcc cagccagtaa aaaacgtcgc aacatcgtgc
      481 atctccttcg tggacttcgg aggattcggc aggtcatagc ccagcttatc tttaaacttc
      541 tttccgtggt ccgggttgct gaggatatct ctccggtaat acagaatctg ggcatccgca
      601 tcgaacaaga ccccgtagag cttgccctgc caagtgtaga gttttttcat ggcaggcaaa
      661 aaatcattcg gatcccaata gggagtcttg gggtctttaa ggaatggctc gatttcgaca
      721 atgtacggct ctttcccggt gaagaaatct ccgtaaaacc aagcgcccgt catgtaggcg
      781 tcataacgac cgagccccgt cgaaagatcg gaaaaaatct gctggtgcag agtgtcgatg
      841 gggacttccg ctacgttgat cttgccgcca gtctgcttct cccagcgcgg tgcatggtat
      901 ttcaatccgt caccaacggt cgccttcaga accatgacgt tgatcgtcgt gcctttgtac
      961 ggcttagatt tcaggaactc aacgcgttcc ttcgtaatag gagcttcgtc gggtacctcg
     1021 ggtgaagagt caagtaagtt agcatcggcc gcgaaagcgg acggtgcgat ccggctcagc
     1081 agggcagcgg aaattccaag tttggtggcc tgctgaataa atctacgccg agtgattctt
     1141 ccttgaaaat acccagctgc taat
//
LOCUS       DATMKH010000021         1205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1617317, whole genome shotgun sequence.
ACCESSION   DATMKH010000021 DATMKH010000000
VERSION     DATMKH010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1617317"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1099
                     /locus_tag="VNV63_00210"
     CDS             <1..1099
                     /locus_tag="VNV63_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61069.1"
                     /translation="VYAGSLSVLNTSPTNTASGIDGPCSLRMSDVNNPLSWNPINQAF
                     LDKDDGTEGTGLASFTITAQGIPPEGSLVVFKNYSAYQIVGIFGSPNLTIQRIKTDMG
                     CLAPRSIQFVPGFGIGRFAHLGFAMFDGVEDRIISEDIRPFLFATSDFSEQDITVLDS
                     NFQAAMWGFQTANPPMFCNAIPIGSSLGMLTRILCYDLVLKAWTAPVDLPFPISTAAQ
                     FRTVTANPVTILGGFSDGLLSRWQAGDQLWDVGAVGARTASNVIWSVKLPEALSQTAD
                     QKLNCRRVAIRGIGTSTSGTITVIPLVNGAQKPSQSYKIPVSGDFEVFASFMRDGLRF
                     SAIISGSGQLELNRFSWHITSKEVGAAALIS"
BASE COUNT          270 a          304 c          334 g          297 t
ORIGIN      
        1 cgtgtatgca ggctctctct cggttctcaa cacttctccg acaaataccg cctcgggaat
       61 cgacgggccg tgctcactac gaatgagcga cgtcaacaac cccttgtcgt ggaatcccat
      121 taatcaggcg ttcctcgata aagacgatgg cacggaaggg accgggctcg cttccttcac
      181 gattaccgcg cagggcattc ctcctgaagg aagccttgta gtcttcaaga actattcggc
      241 gtaccagatt gtcgggatct tcggctctcc gaatctgacg attcagagaa tcaagacgga
      301 catgggatgc ctcgcgccaa gaagtattca gtttgttcca ggattcggta tcgggcggtt
      361 cgctcatcta ggattcgcca tgttcgatgg tgtcgaggac agaatcatta gcgaggatat
      421 tcgtccattt ctatttgcta catcggactt ttcagaacag gacattactg ttctcgattc
      481 caactttcag gcggcaatgt ggggtttcca gaccgctaat cctccgatgt tctgcaacgc
      541 tatccctatc ggaagttcac tagggatgct gactagaatc ctgtgctacg acctagtgct
      601 aaaagcatgg accgctccgg tcgatttacc tttcccgatt tctacagccg cacagttcag
      661 gacggttacg gcgaatccgg taactatcct tggcgggttc tcggacggct tactttcccg
      721 ctggcaggcc ggagaccagt tgtgggatgt cggagcagta ggcgcacgaa ctgcatcgaa
      781 tgtgatttgg agcgtgaaat tgccggaagc gctctcgcag accgccgatc aaaaattgaa
      841 ttgccggaga gtggccattc gtgggattgg aacgagtacc agcgggacga ttaccgttat
      901 tccgttggtg aacggagcgc agaagccatc tcagtcatat aagattcctg tgtccggaga
      961 cttcgaagtg ttcgcctcat ttatgaggga cggacttagg ttctctgcca taatttcagg
     1021 gtcgggccag ttggagctca accgctttag ttggcacatc acatccaagg aggttggcgc
     1081 cgcggcgctg atttcataga tgcaagtaag gaatcttatt gtcggtgaag aacttccgga
     1141 ggaactcagg accggattcg aagcggggcc gtgcactgac ccggagtgga tttgggtggt
     1201 cgaac
//
LOCUS       DATMKH010000022         1228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1617602, whole genome shotgun sequence.
ACCESSION   DATMKH010000022 DATMKH010000000
VERSION     DATMKH010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1617602"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="VNV63_00215"
     CDS             complement(<1..525)
                     /locus_tag="VNV63_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61070.1"
                     /translation="MEENKSAAQRYSSYKKRLMGQPRPRQTLERLEQERMPQALLKVV
                     AEKYANGEHGEADVRAAVYTEFLQNRDRTIIPRQLEELRATIEKLRQELLWAAGRFQH
                     LRDWKTYRVLEYGTWADFLEKELGLSEKVTGLLMMIVGCDRKSSLDTLIQIILKGYVA
                     EPVLNEKQKPQVKRK"
     gene            950..>1228
                     /locus_tag="VNV63_00220"
     CDS             950..>1228
                     /locus_tag="VNV63_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61071.1"
                     /translation="MQDMNEWLRLPDDQSLEAGEDSNEEDPEESNRFSWVPPRLDPRA
                     QAKSVDRNTRPHRADGPVKFYSEEARGQPIFELFEGPDDRLMNRDPENG"
BASE COUNT          282 a          327 c          305 g          314 t
ORIGIN      
        1 tttccgtttg acctgcggct tctgcttctc gtttaaaact ggttcggcaa cataaccttt
       61 cagtatgatt tgaatcagcg tatccaagct gcttttccta tcgcacccaa cgatcatcat
      121 cagaagacct gtcacctttt ccgacagtcc caattccttt tctagaaaat cggcccaagt
      181 cccatattca agaacccgat aggtcttcca gtcccggaga tgttggaacc tacctgccgc
      241 ccaaagcagt tcttgacgga gcttctcgat cgtggcccgg agttcctcta gctgtcttgg
      301 aatgatcgta cggtcccggt tttgcagaaa ttccgtgtag acagctgccc ggacatcggc
      361 ttccccgtgc tcgccatttg catatttctc ggccacgacc ttaagcaagg cttgcggcat
      421 ccgctcctgc tccagccgtt ctaaagtctg ccgaggccgg ggctgaccca tgagccgttt
      481 tttataggac gaatagcgct gggctgcgga tttattttct tccatggccc tcttaattct
      541 gccagggttt attgcttctg tgatcaggtg tccctgtgag ggacaccccg aatagtaacg
      601 ttgatggggg aagataagga gcaatatata tgccagatct tattttgttc tggcggcttc
      661 caggttagag gatttgaaca accccctgtc cctgtgagag acatgtgtcc ctgtgagaga
      721 cacctcacct ggaataccgc tgccataaaa tggttccctc tggccgattc cttatgaccc
      781 gcatccgttc cagcctgtta tcctgactcc gaaagaaaga ttcgctcttt gacaatttca
      841 gacctttctg aaggcggatg gggaaagtct tcttctcttg ctttctcccg ccctttctgt
      901 ggctaaagaa acttggccac agtaaggacg ggggaaagga gaacagcaca tgcaagatat
      961 gaatgaatgg ctgaggcttc cggacgatca atcgctggag gccggagaag acagtaatga
     1021 agaggatccc gaggaatcga atcgattctc gtgggttcct cccaggcttg acccaagggc
     1081 tcaagctaaa tcggtcgacc gtaatacaag accgcacaga gcagatggtc cggtaaaatt
     1141 ctactctgaa gaggcacgag gccagcccat ctttgaactg tttgaaggac cggacgatcg
     1201 gctcatgaac cgggatccgg agaacggg
//
LOCUS       DATMKH010000023         1671 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3221819, whole genome shotgun sequence.
ACCESSION   DATMKH010000023 DATMKH010000000
VERSION     DATMKH010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3221819"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="VNV63_00225"
     CDS             <1..704
                     /locus_tag="VNV63_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004068873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXC61072.1"
                     /translation="SLNSGNGIETGSDREAEKDREILNSIRNSRGNLSKRDGKRLARL
                     RAIEFLRLVRLPEPERVFKMYPFELSGGMRQRAMIAMALVRRPKVLLADEVTTALDVT
                     VQAQILKLLKDLRDKIDSSIILITHDLGVVAETCDRVAVMYAGNIVEVADVFEMFQNP
                     LHPYTKGLFAAVPKPDVTTADLESIKGSVPNLIYPPSGCRFHPRCPFAFEKCPQVKPP
                     LIEARPNHFVACHLY"
     gene            711..>1671
                     /locus_tag="VNV63_00230"
     CDS             711..>1671
                     /locus_tag="VNV63_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013985915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXC61073.1"
                     /translation="METQQQNLIEVKDVYKWFPIKGGLLGKTLANVKAVDGVSLSVKK
                     GETMGLVGESGSGKTTIGRAIMRLTNVTKGEITFDGTDITQLKGRELKPYRRRMQMVF
                     QDPYASLDPRQTIRSTLSEPMKIHHVTASKAESNAAVEKLIETVGLNQDHLSRFPHEF
                     SGGQRQRVAIARALAVNPQFLLLDEPTSSLDVSVQAQILNLLKKLQRDFNLTYLFISH
                     NLSVIRHMCDRVAVMYLGRIVEVGSMQTIYENPKHPYTYALLSSVPNPDPQKRKEMGV
                     LEGDVPSPIDIPSGCRFRTRCPYATNKCTEEFPPLVEIEPGHWI"
BASE COUNT          424 a          438 c          418 g          391 t
ORIGIN      
        1 attctctcaa ttctggcaac gggatcgaaa ctggttctga ccgcgaggcc gagaaagatc
       61 gagagattct gaattccata cggaattccc ggggcaatct cagcaagaga gatggcaaga
      121 gacttgctcg tctccgcgca attgaattct tgcgtctggt caggctgccc gagccggagc
      181 gggttttcaa aatgtatccc ttcgagctat cgggcgggat gcggcagcgg gcgatgatcg
      241 ccatggcgct agtacgccgc cctaaagttc ttctcgcaga cgaggtcacg actgcccttg
      301 atgtgacggt tcaggcacaa attctcaaat tactaaaaga cttgagagac aagatcgatt
      361 cttcgatcat tctcatcact cacgatctgg gagttgttgc ggaaacttgt gatcgcgtcg
      421 cggtgatgta cgctggaaat attgtggaag tggcggacgt ttttgagatg tttcaaaatc
      481 cgctgcatcc ctacactaaa gggctatttg cggcagttcc caagcctgat gtgactactg
      541 cggacttgga atcgataaag gggagtgttc ccaacttgat ctatcctcct tcggggtgcc
      601 gcttccatcc tcgatgccct tttgccttcg aaaaatgtcc tcaggtaaaa cctcctttga
      661 tcgaagctag accgaatcac tttgtagcgt gccatttgta ctgatctacc atggagaccc
      721 agcagcaaaa tctaatcgaa gtcaaagatg tgtacaagtg gtttccgata aagggaggcc
      781 ttctcggaaa aacgcttgcg aacgtaaagg ccgtcgatgg agtctctctg agcgtaaaga
      841 agggagaaac gatgggcctt gtcggagaat ccggttctgg caaaacgact atcggccgcg
      901 cgatcatgcg gcttactaac gtaaccaaag gagaaattac cttcgacggc actgatatca
      961 cccaactcaa aggccgcgaa ctgaaaccct acaggcgcag aatgcagatg gtattccaag
     1021 atccttacgc ctccctggac ccgagacaaa caatccgctc gaccctatct gaaccgatga
     1081 aaattcatca tgttactgcc tccaaagccg agtccaatgc agccgtggag aaactgatcg
     1141 agactgtcgg attgaatcag gatcaccttt ctcgctttcc gcacgagttc agcggcggtc
     1201 aaagacagcg ggttgctata gccagggccc ttgctgttaa tccccagttc ttgcttctgg
     1261 acgaacccac ttcctccttg gatgtttccg tgcaagcgca gatcttgaat ctgttgaaga
     1321 aactgcagcg cgactttaat ctgacctacc tcttcatttc gcacaacctt tcagtaatca
     1381 ggcacatgtg cgaccgcgtc gctgtcatgt acctcggaag aattgtcgag gtgggcagca
     1441 tgcagacgat ttatgaaaat ccaaaacatc catacaccta cgcgcttctt tcatccgttc
     1501 caaatccaga tccccagaag agaaaagaga tgggggtcct agagggagat gttcccagcc
     1561 ccatcgatat tcccagcggg tgcaggttcc ggacgcgatg cccctacgca actaacaagt
     1621 gtaccgaaga gtttccgccc ctagtcgaga tcgagcctgg acactggata g
//
LOCUS       DATMKH010000024         2941 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3542448, whole genome shotgun sequence.
ACCESSION   DATMKH010000024 DATMKH010000000
VERSION     DATMKH010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3542448"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /locus_tag="VNV63_00235"
     CDS             <1..698
                     /locus_tag="VNV63_00235"
                     /inference="COORDINATES: protein motif:HMM:NF012928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HXC61074.1"
                     /translation="SLRKIPVRVQECAGFVVNRLLTPYLNEAAYALQENAAPAKEMDE
                     AMLALGLPMGPFALSDMIGIDVAEKVAHILYDAYGPRMEPAAILGKLVEAKRLGTKSG
                     AGFYNYSNPKDDVVSKMIQEVQKQTGKKSTTFSPTRLLLMMINEAVICLQEDVASAAD
                     IDIAMLAGIGFPQDKGGPLHYADQLGIDLVLNELEKHQKEFGFRFWPAPMLKRMVAAG
                     YLGVKTKRGFFSY"
     gene            714..1496
                     /locus_tag="VNV63_00240"
     CDS             714..1496
                     /locus_tag="VNV63_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006313853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HXC61075.1"
                     /translation="MANQFVNDVVEEHVATLTINNPPMNALTRVVMAELESAIDRVAK
                     DPQIKTVVITGTGNMFVAGADIREIAAISSGKMGAEITAKGQAIYNKIEQLQKPVIAA
                     INGLFCLGGGLELVLACHIRIAGDRARLGLPEIDLGIIPGFGGTQRLTRIVGASKAIE
                     LILTGDKITAQEAKEIGLVNKVVPDADVLKQAQGLAKKIASKGQVAVRSAMKAIGIAI
                     STPLMEGLKKESELFGLVCETADMKEGTSAFLQKRQPKWQDK"
     gene            1652..2749
                     /locus_tag="VNV63_00245"
     CDS             1652..2749
                     /locus_tag="VNV63_00245"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HXC61076.1"
                     /translation="MAGREGKLALVLAGGGMPGWMYEIGCLKALDEFFDDGFTVNDFD
                     IFVGTSAGAAVASLMANLIPPKDIYNAIYENIDHSYNFKSSDIYSFGYQETFFLVKKF
                     LKALFPMIRYIFKNRRQINLIDIYHLLEEFLPSGIFTLQNFDRFLADYFSRDGYTNDF
                     RKLKRELYIPAVDVDMGRYDVFGEGEFADVPISLAVTASSAMPIMFQPVNIRGKDYID
                     GGVSRVAYMDIAINHGATLFLVLNPIVSVVNDRTKVCLQTFFGQPAGLKEKGLSYIYD
                     QSNRISTQTRIYFGLKRYQAEHPQKDFIVLQPNPTDAIMFLYSVINLAAKLEILDYGY
                     RSTVETLKAQFTSYEKCFAKHGIKVTLKRFK"
BASE COUNT          743 a          707 c          820 g          671 t
ORIGIN      
        1 agagtctgcg aaagataccg gtccgcgtcc aggaatgcgc cgggttcgtg gtcaaccgcc
       61 tgttgacgcc ctatctcaat gaggcggcgt acgcgcttca ggaaaatgcc gcgccggcaa
      121 aagagatgga tgaggcgatg ctggccttgg gtcttcccat ggggcctttt gcattgtcgg
      181 acatgatcgg gattgatgtg gccgagaagg tggcccacat cctgtatgac gcgtatggtc
      241 cgcggatgga gccggctgct attttaggca agctggttga agccaaacgg ctcgggacaa
      301 aaagcggggc gggattttac aactacagca acccgaaaga tgatgttgtg agcaagatga
      361 tccaagaggt gcagaaacag acgggaaaga aaagcaccac tttttcccca acccgccttc
      421 tcctgatgat gatcaatgaa gcggtgatct gtcttcaaga agatgtcgcc agcgcggccg
      481 atattgatat cgcgatgctg gcggggatcg gtttccctca agataagggc ggcccgcttc
      541 attatgcgga tcagctggga attgatctgg tgttgaacga gctggagaaa caccagaaag
      601 agtttggttt tagattctgg ccggccccga tgctgaagcg tatggtcgcg gcgggatatc
      661 tcggagttaa aaccaagcgg ggatttttta gttattaata gaggaggatc atcttggcca
      721 accagttcgt aaacgatgtc gttgaagaac atgtcgcgac tctgaccatt aacaatccgc
      781 cgatgaatgc tttaacaagg gtcgtgatgg cagaattgga atcagccatt gatcgggtcg
      841 ccaaagaccc gcaaatcaag acggttgtca tcaccggaac gggcaacatg tttgttgcag
      901 gagcggacat cagagagatc gctgccattt cttccgggaa gatgggggct gaaataacgg
      961 ccaaaggcca ggcgatctac aataaaattg agcagctcca aaaaccggtg atcgctgcga
     1021 tcaatggact tttctgcctg ggaggcggct tggagcttgt gctggcctgc catatccgga
     1081 ttgcaggaga ccgagcacgt cttggtctgc cggagattga tcttggaatc attccgggct
     1141 tcggcggcac gcagcggctg acaaggatcg tcggcgcctc gaaagcgatc gagctgatcc
     1201 tgaccggcga taaaatcacg gctcaggagg cgaaggagat cggcctggtc aacaaagtcg
     1261 ttccagatgc ggacgtgctc aaacaggccc aggggctggc aaagaagatc gcttcaaagg
     1321 ggcaggtcgc ggtccgctcg gcgatgaagg cgatcggtat cgcgatcagc acgccgttga
     1381 tggaaggcct caagaaggaa tccgagctgt tcggtttggt gtgcgaaaca gccgatatga
     1441 aagaggggac atccgccttt cttcagaaaa ggcaacccaa gtggcaggat aagtagtttg
     1501 tcggcaagaa tcctgttcca tccgctgaag agaccaaggt ttctgccacg atttaaccca
     1561 tcccatccat agcccggcct cccggaaccc agaaaataaa aagtctgcta tacttaaagt
     1621 aagggtatag atagaaagag gctgtgaaac catggcgggt cgagaaggaa agttagccct
     1681 ggtgcttgca ggcggcggga tgccgggctg gatgtatgag atcggctgtc tcaaggccct
     1741 ggacgaattt tttgatgacg gcttcaccgt caacgacttt gacatcttcg ttggaaccag
     1801 cgccggtgca gctgtggcct cattgatggc caacctgatc cctcccaagg acatctataa
     1861 tgcgatttac gagaacatag accattccta caattttaag agcagcgata tctacagctt
     1921 cggctaccaa gagacctttt ttctcgtaaa gaaattttta aaagcgttgt ttccgatgat
     1981 ccggtatatt tttaaaaatc ggagacagat caacctcata gatatttatc acttgctgga
     2041 ggagttcctc ccttccggaa ttttcacctt gcaaaacttt gaccgtttcc ttgcagacta
     2101 tttttcacgg gatggataca ccaacgattt ccgcaagctt aagcgtgagc tctatatccc
     2161 cgctgtggat gtagatatgg gaagatacga tgtcttcgga gaaggggaat ttgcggatgt
     2221 ccccatctca ctggcggtca cagcctcttc cgctatgccg atcatgtttc agccggtaaa
     2281 tatccgggga aaagactata tcgacggcgg tgtctcacgc gtcgcttaca tggacatcgc
     2341 aatcaatcac ggagcaactc tgttcctggt cttgaacccg atcgtcagtg tggtcaacga
     2401 ccggaccaaa gtctgccttc agaccttctt tggacagccg gcaggattga aagagaaggg
     2461 gctgtcttac atttatgatc agtccaaccg tatcagcacc caaacgcgaa tctactttgg
     2521 cctaaagcgt taccaggctg aacacccgca gaaggatttt atagttctgc agccaaatcc
     2581 gactgacgca atcatgtttc tgtacagcgt gatcaacttg gcggctaagt tagagatctt
     2641 ggattatggt tatcgctcaa ccgttgaaac gctcaaggcc cagtttacga gctacgaaaa
     2701 gtgttttgcc aagcacggga tcaaagtgac gctcaaacgg tttaaatagt aagatcgggt
     2761 cggtgacccg cctgggcgag gcatcgcctc gcccctactg gatcttcggc ggccagatca
     2821 cgtagagcat gaagtacgtg gctgtgctgg taaatagggc gattacgaag agggtcatcg
     2881 taaaggttcc gatgagccgg tgacgccgct ccccatccgg aataaagcgt tcgatcactt
     2941 t
//
LOCUS       DATMKH010000025         1009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2902298, whole genome shotgun sequence.
ACCESSION   DATMKH010000025 DATMKH010000000
VERSION     DATMKH010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2902298"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..789)
                     /locus_tag="VNV63_00250"
     CDS             complement(<1..789)
                     /locus_tag="VNV63_00250"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HXC61077.1"
                     /translation="MKSNGTLPSIPHPNREQALKSARLAAGFPNIVNGKRIDSLKSIT
                     ISDPVTGEELARVSETQKDGLDRAVEAAKAAFPAWSSKTWNERRDLLRNAMEELKAHT
                     DELCTILTAENGRPYSMAQWEITWIFETYVPSLLQMELPDSTWNETGVGQITKRYVPL
                     GVVAAISPWNLPFLLSFTKVLPSLLAGNTVVLKPAVFTPLTVLRAADYIREILPPGVL
                     NVISGGDDLGPWMTSHPDFQKVSFTGSSETGCKVFASAAPTLKHL"
BASE COUNT          229 a          266 c          273 g          241 t
ORIGIN      
        1 caggtgtttc agggttggag cggcggatgc aaaaaccttg catccggttt ccgaagatcc
       61 tgtgaaggag accttctgga agtccgggtg ggacgtcatc caggggccaa ggtcatcccc
      121 tccgctgatc acattcaaaa caccgggcgg aaggatctca cgaatgtagt cggcggctcg
      181 aagtaccgta aggggggtaa agaccgcagg cttcaggaca acagtgttgc cagccagcag
      241 agatggcaac acttttgtga atgacagcaa gaacggaaga ttccacggac tgatcgcggc
      301 aacaacaccc agtggaacat aacgcttcgt gatctgtccg acaccggttt cattccatgt
      361 cgaatctggc aattccatct gcagtaacga cggtacatag gtctcgaaga tccatgtgat
      421 ctcccattgc gccatgctat agggccggcc gttttccgct gtcaggattg tacaaagctc
      481 atcggtatga gccttgagct cttccatagc atttcgaaga agatcccttc gctcgttcca
      541 ggtcttgctg ctccaagcag gaaacgctgc ctttgcagcc tcgaccgccc ggtcgagacc
      601 atctttctgg gtctcggaca cgcgtgcaag ctcttctccg gtcacaggat cggagatcgt
      661 tattgacttc aaagagtcga tccttttgcc gttcacaata ttcggaaacc ccgcggccag
      721 acgagcgctt tttagcgctt gctcgcgatt gggatggggt atagatggga gagttccatt
      781 tgatttcata caaaaaaggt cctttcgcta agccgttatg aggagcgcct tcttccgcgg
      841 ctaaccttga gttctggata agcctcaggt gcgcgtagag tgagttcctc gatgttagga
      901 gagatcaggt cgagcttggc actgcctgac ggcgccatcc actcgcgatc aattaaagac
      961 ttcgaacgga ctcgtctgag atctcgtgtt gtgcgttaac gctacttcc
//
LOCUS       DATMKH010000026         1092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4186359, whole genome shotgun sequence.
ACCESSION   DATMKH010000026 DATMKH010000000
VERSION     DATMKH010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1092
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4186359"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..683
                     /locus_tag="VNV63_00255"
     CDS             135..683
                     /locus_tag="VNV63_00255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXC61078.1"
                     /translation="MQISDHTFDDWVRQYQRLLFGIAYWWTGSRTDAEELTQEAFFQA
                     YRSRSSLREIEAVKGWLVGILRHCYAQMFRKGHSRIEIPLDEVMEKSGETIDLRSDGP
                     DPHDFLALRQALERLDERHRLPLVLFYFQELSYREIAEALELPLGTVMSRLSRARQLL
                     HECLTRPVALRFVKKIRKVNGL"
     gene            680..>1092
                     /locus_tag="VNV63_00260"
     CDS             680..>1092
                     /locus_tag="VNV63_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61079.1"
                     /translation="MNCDLYRSEMYAWRPGSDEKSFQPLFDHLDSCAECARLFEQLTA
                     SDENVRRTFQSYPQSPFLESRILSGLVHQRAQASARRAGWRLWMLAPALVPLLIVLFV
                     WVAPRFEQARLNREMAILLSKPPALEINSTDRKQLL"
BASE COUNT          242 a          301 c          298 g          251 t
ORIGIN      
        1 cttcaccctc tcagtgcgga atgaggcgcc acactttcgg gaaaagttca gcaatcacgt
       61 catcagaagg aataaaacca tcggatgccg ccactcttat gggtgacagc gctccgggag
      121 cacaataaga gcaagtgcag ataagcgatc acacttttga tgattgggtc cggcaatacc
      181 aaaggcttct ctttggcatc gcctactggt ggaccggctc gcggacggat gccgaggaac
      241 tcacgcagga agctttcttc caggcttatc gatcacgttc cagtctccga gagatcgagg
      301 cggtgaaggg ttggcttgtt ggcatcctcc gtcactgtta tgcccagatg tttagaaaag
      361 gccactctcg gatcgagatc cctctcgatg aagtgatgga gaaatccggc gaaacaatag
      421 atctacgatc ggacggccct gatccacacg attttctagc gcttcgccaa gctcttgagc
      481 gattggacga aaggcatcga ctgcccctgg ttctctttta ttttcaggag ctctcttatc
      541 gagagattgc cgaggctctg gaactgccgc tcggcaccgt catgtcccga ctttcccgtg
      601 cccgccagtt gctgcacgaa tgcctcacca ggccggtcgc gctgagattt gtgaagaaga
      661 tcaggaaggt gaacggacta tgaactgcga cctgtacaga tcggagatgt atgcctggcg
      721 tcccggcagc gacgaaaaaa gttttcagcc cttgttcgat cacctggaca gctgcgccga
      781 atgtgcccga ctctttgagc agctgacagc ctcggatgag aacgtccgac gtacatttca
      841 aagctatccg cagagccctt tccttgagag ccggattctt tccggattgg tccatcagcg
      901 tgcgcaggcc tctgcccgtc gagcaggctg gagattgtgg atgctcgcgc cggccttggt
      961 tcctttgctc attgtgctct ttgtgtgggt tgcaccccgg ttcgaacaag caaggctgaa
     1021 cagagagatg gcgattctcc tgagtaaacc gcccgctctt gagatcaaca gcacggatcg
     1081 gaagcaactc ct
//
LOCUS       DATMKH010000027         5203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2582840, whole genome shotgun sequence.
ACCESSION   DATMKH010000027 DATMKH010000000
VERSION     DATMKH010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 5203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5203
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2582840"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1753)
                     /locus_tag="VNV63_00265"
     CDS             complement(<1..1753)
                     /locus_tag="VNV63_00265"
                     /inference="COORDINATES: protein motif:HMM:NF028237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HXC61080.1"
                     /translation="MIPKVTLLFITVLLNLSLVLTIYLRGRRNPINVFYNLIVLSVAV
                     WAFCYGMLELSTQPGDALFWYYLTYVAGSLIASFFFYFSFVFPTQERSFTAADHLLAW
                     GFPIVSLCLLADPHILIREIALEPSGWSVVLGKPAFWTYSFWLTLLMGRAFLHLYQKY
                     QRALGRPKMQLRYVLFGTAISTFFGSIFNLIMPGMGDTNWIWLGPLFEIILVGSTAYG
                     IVKHRLMDIRVIFNRAIVYSIFLIITLALYTMVVLTSQQFFKDTTGVVASLIIGSVLI
                     ALGLEPLRKLIQKTTDRIFFKGEFVTQQLLGTITDTLSSIDDLERRLHIVTAHLATDL
                     RVERAAFWIFGQDQNQITLKILEGSPMPSENLSPNAILQYFTATSVGEYVMVSQRRES
                     LLYDETESPLSEQMDPDPILTTVFEEMKQLGMELIIPIFSKDILVGLLLMGSKRSGEL
                     YFNDDLKLLGIIAQEAGIAIDNARLYHRLQQQMEELKTMQTHQLLQSAKLATIGELAT
                     NIAHEINNPLTSILGFTTLLLKETGESDPRRKDLKIIESEAMRSRDIVRNLLDFARSK
                     EPKKEAVGINEVVHDTLA"
     gene            complement(1750..2913)
                     /locus_tag="VNV63_00270"
     CDS             complement(1750..2913)
                     /locus_tag="VNV63_00270"
                     /inference="COORDINATES: protein motif:HMM:NF013641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61081.1"
                     /translation="MFSIFRMLRDLALRWLPISVSAHMVPADFVFIVHPRDIPDVARQ
                     YAWARFLPARLVAVLTRNLWPILGPKITGATTKAGNPLRGQVIFVPMVPDQMFKDLHL
                     ARKRVYQAIALGEHLGPKVIAVGGLCPAIIRGKWKLLDSVKPLITTGRIYSSVLAAQD
                     LTRVAERIGLNLPSATIAVVGAAGATGALVSKLLVEQIGRLILIDRRLNVLKDVSDHL
                     RQIRPTIQIELSSEIKSIETADAVVAAASSAGAIIEPHHLKTGTVIVDCGQPHNVTED
                     LMAKRQDIVVVEGGLAKLAGIKCQFDFGLLAEDELFGCLSEAVLLCWSEKYKRHGFEG
                     RSELQLAKEFFEIGKMLGFLRPPLRFGSHAITDAELDRVRDVRRVKKDQSKTP"
     gene            3194..3457
                     /locus_tag="VNV63_00275"
     CDS             3194..3457
                     /locus_tag="VNV63_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61082.1"
                     /translation="MIGPLLLAILFWVIYFFIIFFICHWLYQRLSGPEPKPHVPPVIV
                     QNKSIPAREIWERRAKRLQRLFIIVAVILMVVPLFLPFVLPHF"
     gene            3567..3968
                     /locus_tag="VNV63_00280"
     CDS             3567..3968
                     /locus_tag="VNV63_00280"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HXC61083.1"
                     /translation="MATLGQLVNRKIQAVKQDTTIREAAELMKRQRIGSLLVEKDGQY
                     VGIVTETDIVRKAAAQGLDLKKEKVITIASSPVITLDIYRTPQDAHDLMGEVGVRHLG
                     ISDKGKVIGIVSVRDLLVFFKKQSEPRMGID"
     gene            complement(3998..4420)
                     /locus_tag="VNV63_00285"
     CDS             complement(3998..4420)
                     /locus_tag="VNV63_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HXC61084.1"
                     /translation="MPRFPVSPAKEKQLIEQMQRLGIREEDLAETFVRSSGAGGQNVN
                     KTSTCVVLLYQPTGLKIKCQTARSQGLNRFLARRLLLDRIEQERLGTRSAEQQRTEKI
                     RRQKRRRSRRARNKMLADKRHRSDKKSLRAAVRSGNEE"
     gene            complement(4430..4972)
                     /locus_tag="VNV63_00290"
     CDS             complement(4430..4972)
                     /locus_tag="VNV63_00290"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXC61085.1"
                     /translation="MGIKHIDHIAFRVADLEKAIRFYTEVMDFKITDRFSIDFEDSTK
                     ARCAALQAKDEKGISIFLSEGEGEGGVVKEWVKQHGNTLHHIAYAVDDIKTEVAEMKR
                     KGIPFTTDEILESEELLQIFTKPAPETGVIHEVIQRKGTKSFSTTNIKRLMNSTKDLE
                     KSEKTKAAKKNNADRKTSKT"
     gene            complement(4990..>5203)
                     /locus_tag="VNV63_00295"
     CDS             complement(4990..>5203)
                     /locus_tag="VNV63_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015129488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HXC61086.1"
                     /translation="MHAIRDMETGITYINGPTIGAEVHLPFGGLKHTGNGHREAGSPG
                     LDIFSEWKAVYIDYSGRLQKAQIDVD"
BASE COUNT         1225 a         1318 c         1381 g         1279 t
ORIGIN      
        1 aggccagcgt atcgtggacg acctcgttaa ttcctacggc ttcttttttt ggttctttgc
       61 tgcgtgcaaa gtccaatagg ttacggacaa tatctctgga ccgcatcgct tcgctctcga
      121 tgatcttgag atcttttcga cgagggtctg actcgccggt ctctttcaat aaaagagttg
      181 tgaatcccag gatgctggtc aaaggattgt tgatctcatg ggcaatattg gttgcaagct
      241 caccgatcgt ggccagcttc gcagactgga ggagctgatg ggtctgcatt gtcttaagct
      301 cttccatctg ctgttggagg cggtggtaaa gccgggcatt gtcgatggcg attccggcct
      361 cctgggcaat aatcccaagc agtttcagat cgtcgttgaa gtagagctcc ccggaccgct
      421 tggatcccat caaaagcaga ccgaccagga tatctttgct gaagatcgga atgatcaact
      481 ccatgccaag ctgtttcatt tcttcaaaga ccgtggtgag tatcggatcc gggtccattt
      541 gttctgataa cggactttcc gtctcatcat acaacaatga ctcccggcgt tgtgagacca
      601 tgacgtactc ccctaccgaa gttgcagtga aatattggag gatggcgtta gggctcaaat
      661 tctccgaggg cattgggctg ccttctaaaa ttttgagggt gatctgattc tggtcttgtc
      721 caaagatcca gaaagctgct cgctccacgc gaaggtctgt agcgaggtga gccgtaacga
      781 tgtggagtcg tctttcgagg tcgtcgatcg agctcagggt gtctgtaatc gttcccaata
      841 attgttgggt cacaaattca cctttaaaaa agatacggtc ggttgtcttc tggatcaatt
      901 ttctcaaagg ctccagcccg agtgcgatga ggaccgaacc gatgatgagg ctggccacaa
      961 cgcccgtggt gtctttaaag aactgttgtg aggtgaggac gaccatcgta tagagtgcca
     1021 gcgtgatgat caggaagata gaataaacga ttgctcgatt gaagatcacc cggatgtcca
     1081 tcagacggtg cttgacaatt ccgtacgcgg tggacccgac cagtatgatc tcaaacaacg
     1141 ggccaagcca aatccagttt gtgtctccca ttccaggcat aatcagatta aagatgctgc
     1201 cgaagaaggt agaaatggcg gttccaaaca ggacataccg taactgcatc ttaggacggc
     1261 ccagcgcccg ttgatatttt tgataaaggt gaagaaaggc gcgtcccatc aagagcgtaa
     1321 gccagaagga gtaggtccag aaagccggtt tgcccaagac gaccgaccat ccggaaggtt
     1381 caagtgcgat ctcacggatc aggatatgag gatcggccag tagacagagc gacacgatcg
     1441 ggaaacccca tgccaggaga tgatccgcag cggtaaagga ccgttcctga gtgggaaaga
     1501 cgaatgaaaa gtagaagaaa aagcttgcaa tcaaggatcc agccacatag gttaaatagt
     1561 accagaagag ggcatcccct ggctgggtgg acagctccag cattccgtag caaaaggccc
     1621 agaccgcgac agaaaggacg ataaggttgt agaagacatt gatcggattc cgccggcctc
     1681 ggaggtaaat ggtcaaaacc agcgagagat tcaggaggac cgtgatgaac agcagagtaa
     1741 cttttggaat catggtgttt tagattgatc cttcttgacc cttcggacgt cccgaacccg
     1801 atccaattcc gcatcggtga tggcatgact gccaaaccgc aagggtggac gtaagaaccc
     1861 gagcattttc ccaatctcaa aaaactcttt tgccagctgg agttccgaac gcccttcgaa
     1921 accatgccgt ttgtattttt cggaccaaca gaggaggacg gcttcactga ggcaaccgaa
     1981 cagctcatcc tctgcaagca agccaaaatc aaactgacat tttatccctg caagcttggc
     2041 cagaccacct tcgacgacca cgatgtcctg gcgcttggcc atgagatctt cggtgacatt
     2101 gtggggctgc ccgcaatcta caatgacggt ccccgtcttg agatggtgtg gttcaatgat
     2161 cgccccggct gagctggcgg ccgcaaccac cgcgtcggcg gtttcaattg acttgatttc
     2221 cgaggagagt tctatttgaa tcgttggacg tatctgtcgc aggtgatcag atacatcttt
     2281 gaggacattt aatcggcggt caatgagaat cagacgcccg atctgttcca cgaggagttt
     2341 gctcaccaga gccccggtgg cgccagccgc gcccaccacc gcaattgtgg cagacggaag
     2401 attcagaccg atgcgttcgg cgactcgagt caggtcttgg gcggcgagga ccgatgagta
     2461 aatgcggccg gttgtgatca acggcttgac cgaatccaga agtttccact tccctcgaat
     2521 aatcgcagga catagacctc ctaccgcgat gaccttcggt cctagatgtt ctcccaaagc
     2581 gatggcctga tacacacgtt tccgagcgag atgcaggtct ttaaacatct gatcaggaac
     2641 catggggacg aagatcactt gaccacgtag cggattcccc gctttggtgg tcgcccctgt
     2701 gatctttgga cccaagatcg gccagagatt tcgtgtgaga acagccacca gacgtgcagg
     2761 caagaagcgg gcccacgcat actgccgagc gacatccggg atatcccgag gatgaacgat
     2821 aaagacaaaa tccgccggga ccatgtgggc tgaaacggag atggggagcc atcggagagc
     2881 aagatccctt aacattctga atatgctgaa catcgccacc tcacctcgat gattttaatt
     2941 taatgttaaa ccgtatgatg gagatcgaaa tctccccgct tttaaacaaa atataccaga
     3001 ccgttacctc tttggcaagg cgcccttgta tcatttacat ctgttaatgc cctgtgctaa
     3061 catgcaacca agagccattt aaattcaagg agaagagagg cccgcggatt cttgcgttgc
     3121 aaccgcaaag acaccgcctg cctcatctga gtagctgacg aaactcttta attatggtta
     3181 gaaggggtaa gggatgatcg gaccgctgct cttggccatt ctattctggg tgatttactt
     3241 tttcattata ttttttatct gccattggct gtatcaacgg ctcagtgggc cggaacccaa
     3301 accccatgtc ccgcccgtga ttgtccagaa taagtccatt cctgcaagag agatctggga
     3361 acggcgtgcc aagcgccttc aaaggttgtt catcatcgtg gcggtgatcc tgatggttgt
     3421 tccgttgttt ctgccttttg ttctgccaca cttttaattt aaacagtaaa tcgagccatt
     3481 tttgagtcag ttgcggggaa gcctcgattg atatatactt tacggcatcg gatcggataa
     3541 ggaggttggc aaagaaggag gattgcatgg cgacgttggg acagttggtg aaccgcaaga
     3601 tccaagctgt taagcaggac accacgattc gtgaggcggc ggagcttatg aaacggcagc
     3661 ggatcggatc acttctggtt gaaaaagacg gccagtatgt gggcatcgtg accgagaccg
     3721 atatcgtccg aaaggcagcg gcccagggcc tcgatctcaa gaaagaaaaa gtaatcacga
     3781 ttgcaagcag cccggtgatt acgcttgaca tctatcggac tccgcaagac gcacatgacc
     3841 tgatgggaga agtcggcgtg cggcatcttg gtatcagcga taagggcaag gtcatcggga
     3901 tcgtctcggt ccgcgatctt ctggtcttct ttaaaaagca gtccgagccc cggatgggga
     3961 ttgattgatg acgatcacaa ggatcgaccc tgcccgccta ttcttcatta ccagaccgta
     4021 ccgccgcgcg tagacttttt ttgtccgacc ggtgacgctt gtcggccagc attttattgc
     4081 gcgcccgccg ggaacgcctg cgcttctgac gacgtatttt ctcggtccgt tgttgctcgg
     4141 cactccgtgt acccaaccgt tcctgctcga tccggtccag taatagccgc cgcgccagaa
     4201 aacgattgag cccttgagaa cgcgcggtct ggcattttat cttgaggccg gtcggctggt
     4261 agagcagcac cacacaggtc gaggtcttat tgacattctg gcccccggcg ccggaagaac
     4321 gtacgaacgt ttccgcgaga tcttcctcac ggatgcccag tctctgcatc tgttcgatca
     4381 gttgtttttc tttggctggc gaaacaggaa agcgcggcat ggggtttggt taggttttgg
     4441 aagtttttcg atctgcatta tttttcttgg cggccttggt tttctcgctc ttttccaggt
     4501 ctttagttga gttcatgagg cgtttgatgt tggtcgtaga aaaactcttg gttccctttc
     4561 gctggataac ctcatggatg acgccggtct ccggggccgg cttggtgaag atctgaagaa
     4621 gctcctcgct ctctaaaatt tcgtctgttg tgaacgggat gccttttcgc ttcatctcag
     4681 cgacctcggt cttgatgtca tctacggcat aggcgatgtg atgcagagtg ttgccgtgct
     4741 gtttcaccca ttccttgacc acgccgcctt cgccttcccc ttcgctcaag aagatcgaga
     4801 tccccttttc atccttggct tgaagggcgg cacagcgggc ttttgtgctg tcctcaaagt
     4861 caatggagaa gcgatccgtg atcttaaaat ccatgacctc ggtgtagaac ctgattgctt
     4921 tttccagatc ggctacacgg aacgcgatgt ggtcaatatg tttgataccc atgcggctac
     4981 ccctccttat taatccacgt caatctgggc cttctggagt ctgcctgaat aatcgatata
     5041 gaccgctttc cattccgaga agatatccag gccgggcgat cccgcctccc ggtggccgtt
     5101 gccggtatgt ttcaagccgc caaacgggag atgcacttcc gcgccgatcg ttggaccgtt
     5161 gatatatgtg attccggtct ccatgtcccg gatcgcatgc atc
//
LOCUS       DATMKH010000028         2604 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1300026, whole genome shotgun sequence.
ACCESSION   DATMKH010000028 DATMKH010000000
VERSION     DATMKH010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1300026"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1582
                     /locus_tag="VNV63_00300"
     CDS             <1..1582
                     /locus_tag="VNV63_00300"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXC61087.1"
                     /translation="GIGIPKEHLGKIFERFSQVDASASRQHEGTGIGLALVKELVELH
                     HGQVKAESEPGRGTVMTVTLPMDLKEAEEKEALTPAQIERRAENRRRTEGRREDDWTK
                     TLHHAAEYSAMDIGSDSPQMSPAETSGETIDAPRSTSIHRVLVVEDNADMRNFVAFQL
                     KDDYVVLTARDGFEGVKAAKLTVPDLIVSDVMMPGKDGYQLCQEIKADAKTKHIPVIL
                     LTARAELSEKISGLERGADDYLTKPFNTQELKAKIKSLISLRGLEREIQSRSVELEGA
                     LKELQETQAQLVHSAKMASVGYLVAGVAHEINNPISFAKGSINSLKRCLDEVKGNIKD
                     PEALQDMEKAITIIRTGLERTEQIVWDLKNFARKDETFFKAHDLHEGLDATLNLLSSS
                     MEDRITVHKEYGPIGPVEMVAGQINQVFMNLLQNSIQSIADRGDIWIRTEKVNSPSGD
                     EVIISVKDNGAGIKAEHLEKIFDPFFTTKQVGKGMGLGLSISYKIIETHGGTMEAKSQ
                     EGHGTEMIIHLPVMQQRS"
     gene            1694..2248
                     /locus_tag="VNV63_00305"
     CDS             1694..2248
                     /locus_tag="VNV63_00305"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HXC61088.1"
                     /translation="MSYLQKKLQKITFKTIPVYFGAVLLAIFAKPTLPFFLAGLALVI
                     PGELIRIWAAGHLRKNQEVTTTGPYAYVKNPLYLGTLLIMIGYCLMARNLILLLIGLA
                     VFSLYYVPFKRHRESARLQERFGDSWTEYDWAVPDYLPRLSPYPKRGAHPWRWKVFLD
                     NSEHETAIVVLLMIILIGLRFWLR"
     gene            2286..>2604
                     /locus_tag="VNV63_00310"
     CDS             2286..>2604
                     /locus_tag="VNV63_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61089.1"
                     /translation="MLSGLTRDSLLIHTVEVLRQEKPWKADILLVQVNGQRAVVKDYA
                     RRGWLYRFYGVWSTWREAYFYQKLAGLPGIPAYCGKLDRYALVIEYIPGKNASKYRPG
                     ELPS"
BASE COUNT          643 a          652 c          728 g          581 t
ORIGIN      
        1 cgggatcggg attccaaagg agcatcttgg aaagattttt gagcggttct cacaggtgga
       61 cgcctcggcg agccgccagc atgagggaac cgggatcggc ctggctcttg tgaaagagct
      121 ggtggagctc catcatggcc aggtgaaggc cgagagtgag ccgggtagag ggaccgtgat
      181 gaccgtgacc cttccgatgg atcttaaaga ggcagaagag aaagaagcgc tcacgccagc
      241 ccagattgag cggcgagctg aaaatcgtcg aaggacagag gggcggcggg aagatgactg
      301 gacaaagacg ctgcaccatg ccgccgagta cagcgcgatg gacatcggct cggacagccc
      361 gcagatgtct ccggcagaga catccggaga gacaatagat gccccgcgat cgacctcaat
      421 ccatcgggtc ctggtagtag aggacaatgc cgacatgcga aactttgttg cgtttcagct
      481 caaggatgat tatgtcgtct tgaccgcccg tgatggattt gaaggggtga aggccgccaa
      541 gcttacggtt cctgacctga tcgtctcgga cgtgatgatg cctggaaagg acggttatca
      601 actctgccag gagattaagg ccgatgccaa aaccaaacat atcccggtga tcctgctcac
      661 ggcccgagcg gaactttccg agaaaatctc gggactggaa cgcggggcgg atgattacct
      721 caccaagccc ttcaatacac aggagttgaa ggcgaagatc aagtccctga tcagcttgag
      781 aggtctggaa cgcgagatcc aatctcgaag cgtggagctg gaaggggcgc tgaaagaact
      841 ccaagagacg caggcccagc ttgtgcactc cgctaagatg gcctccgtgg gatatctggt
      901 tgccggggtg gctcatgaaa tcaacaaccc gatcagcttt gcgaagggga gcatcaatag
      961 tctcaaacgc tgccttgatg aggtgaaagg caatataaaa gatcctgagg cgctgcagga
     1021 tatggagaag gcgatcacca tcatccgaac cggcctggaa cgcaccgagc agattgtatg
     1081 ggatcttaaa aactttgccc gaaaagacga gacttttttt aaagcccatg atctccacga
     1141 aggactggat gcaacgctga accttctgag cagcagcatg gaagatcgga tcacggttca
     1201 caaagagtac gggccgatcg gcccggtgga gatggtagcg ggccagatca atcaggtgtt
     1261 catgaattta ttgcaaaatt ctatccagtc gattgcggac cgaggggata tctggatccg
     1321 gacagagaaa gtgaattcac cttccggtga tgaggtcatc atatcggtga aagacaatgg
     1381 cgcgggcatc aaagccgagc atctcgaaaa gatctttgac ccgttcttca ccacaaaaca
     1441 ggtcggcaaa ggaatgggtc tgggcctctc catcagctat aagatcattg aaacccacgg
     1501 cggcacgatg gaagcgaaaa gccaggaagg ccatggcacc gagatgatca ttcatctgcc
     1561 cgtgatgcag cagcggtctt aatttattat gatactcctc atgaagccca gccgttttta
     1621 attgcaattc atcgcttgct ctgctattct tccctgcctg tccgcggatg ggaataattt
     1681 tacggaagcc atcgtgagct atcttcaaaa gaagcttcaa aaaatcacat tcaagacaat
     1741 accggtctat tttggtgcgg tgctcctggc gatttttgcc aagccgacct tgccgttttt
     1801 tcttgccggg ctggccctgg tgattcccgg ggagctcatc cgaatctggg cggcgggtca
     1861 tctccgaaag aaccaggagg taaccaccac cggaccttat gcttatgtca aaaacccgct
     1921 ctacctcggc acgcttctca tcatgatcgg ctactgcctc atggctcgaa acttgattct
     1981 tctccttatc ggtcttgcgg ttttctctct ctactatgtc ccatttaaaa ggcatcgtga
     2041 atcggcgcgg cttcaagagc ggttcggaga tagctggacc gagtatgact gggctgttcc
     2101 tgactacctc ccccgcttga gtccttatcc taaacgcggt gcgcatccgt ggcgatggaa
     2161 ggtttttttg gataacagcg agcatgaaac tgcgatcgta gttctcttga tgatcatcct
     2221 catcggattg agattttggc tgcgatagat tataatgtcg ctgttttcaa agagaagaaa
     2281 attaaatgct ttccggcctg acacgcgact cgcttttgat tcataccgtt gaagtgctcc
     2341 gtcaggaaaa gccctggaag gcggatatac tgctcgtgca ggtcaacggt caacgggccg
     2401 ttgtcaagga ctatgcccgt cgcggctggc tgtaccgttt ttatggagta tggtctacct
     2461 ggagagaggc ctatttctat cagaagcttg caggtctgcc cggtatcccg gcctactgcg
     2521 gcaagctgga ccgatatgcc ctggtgatcg agtacattcc cggcaagaac gcttcaaaat
     2581 accggccagg ggaactgcct tctg
//
LOCUS       DATMKH010000029         1032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_338622,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000029 DATMKH010000000
VERSION     DATMKH010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338622"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            382..>1032
                     /locus_tag="VNV63_00315"
     CDS             382..>1032
                     /locus_tag="VNV63_00315"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXC61090.1"
                     /translation="MKFGPWAIVTGASSGIGKEFSRQLAANGLNIVLAARRLELLKEL
                     GQNLEKEFGVQCRAVQVDLSDENFIGTIKEATQDLDIGLVVSNAGTGNPGSFIKKNRD
                     DLLMDVRLSVTAHLELCHLFAPILVKRGHRGILLVGAMGASHGVPYMANSGAAKAYVS
                     SLGQALHFELKDQGVHVTVLTPGFIDTPVIPKLLEDPGALPMKPMSAERCVTEGLKA"
BASE COUNT          250 a          268 c          285 g          229 t
ORIGIN      
        1 ggctgagggc cggacctcgg gtgccacggg cgctgcggcg aagctaggcg tacccagaca
       61 gacgcttgaa tccaagatca ggaaactcgg aattaaccgc catcgcttca gaacgtcgta
      121 atctccacct ggccattagg cctcgtttgg atttaaaccc gcttccttct ctctcaatcc
      181 cgcgtatcgc gcctcttcga tacgccgccg caacaggatt cggggggagc caacctcgcg
      241 ggcgatgctc cattttcggc aagcgctgaa attgcggcat gactggaaaa gctaaagaca
      301 aatggctcaa cagattgcga gtggcactga tattgccttc ctcagcctca tatgaatcag
      361 agtctacaaa ggatcgacaa ggtgaaattt ggaccctggg ctatcgtaac gggtgcctcc
      421 tcagggatcg ggaaagaatt ttcaaggcaa cttgccgcga acggcttaaa catcgtgtta
      481 gccgccagaa ggctcgaact gcttaaggag ctgggacaga atctcgagaa agagtttggc
      541 gtacagtgcc gagcggttca agtggatctc agcgatgaaa attttatcgg tacaatcaaa
      601 gaagctacgc aggatctcga tatcggtctt gtcgtttcaa atgctggcac cggaaatccc
      661 ggatctttta tcaaaaagaa tcgagatgat ttactgatgg atgtgagact cagtgtgacg
      721 gcccacctgg agctgtgtca tctctttgcg cctatcctag tgaagcgcgg acaccgcggg
      781 attcttctcg ttggagcgat gggagcgagc cacggggttc cctatatggc gaatagcggt
      841 gcggctaagg cgtatgtttc gtcgttaggt caagcccttc acttcgaatt gaaagaccag
      901 ggagttcatg tgacggtcct aactcccggc tttatcgata cccccgtgat tcccaagctc
      961 ctcgaagacc ccggagcgct cccaatgaag ccgatgtctg ccgaaagatg cgtgacagag
     1021 gggctcaaag cg
//
LOCUS       DATMKH010000030         1615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2583675, whole genome shotgun sequence.
ACCESSION   DATMKH010000030 DATMKH010000000
VERSION     DATMKH010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2583675"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(174..1553)
                     /locus_tag="VNV63_00320"
     CDS             complement(174..1553)
                     /locus_tag="VNV63_00320"
                     /inference="COORDINATES: protein motif:HMM:NF015736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HXC61091.1"
                     /translation="MEIRDIDTGLPAAIDCERTVLGAILNDNEAFFDDTAIIESDDFH
                     LDSHRRIYIVMTEILFGMIEGAKSCDIVTLANELRKRNWVESVGGVAYLAGLTEGLPR
                     RPVITEYVRIIKDKALLRRIMSVCTVGAARAADQSMPAIEVLGSVQVDLAEIVGEGIN
                     NAVDIGSITPAVESSVLKARQITDDRTALEMTWGHPKMDAETRGCFRGEFSVLSGESS
                     GGKTAFAVQVTIANAREGTPVLWFSLEMTKEQLARRFYASMSDKLTAAQMRDPRLMNQ
                     HTHIPAMHEVSKELSRLPIKIDDTTPLRIDKLRARIRMMRRKLAPRPDDKLLVIIDYL
                     QLIRGMAKMSPQEKTELIIFTLRDLPKVEPNIHILALSQYSQGEKIAVKNKRRSKDAL
                     FGGSVIHHAAQNIFMLSIEDPEKRDPRDLLDAEIKIEKQREGKRTKVTTSFDRDHLTF
                     ADPQPYLRS"
BASE COUNT          335 a          456 c          382 g          442 t
ORIGIN      
        1 tatcgcgttt gagggcaaat gcgtgatacc cgatcttcag actgttgacg gataccagac
       61 caccagcctt gcagatgatg ctccaatgct ttttggcttc ctgtttgcgg ttttcagctt
      121 cttcacttac cggctgaagg atactgatag gttcgactgc tgcggccatt gagctaactc
      181 cttagatacg gttgtgggtc tgcgaacgta agatgatccc ggtcgaacga tgttgttacc
      241 ttcgtccttt ttccctctct ctgcttctcg atctttatct cagcatcgag caagtctctg
      301 gggtctctct tctccgggtc ttcaatcgaa agcatgaaaa tgttctgagc cgcatgatgg
      361 atcacggacc caccaaacaa cgcatccttc gaccgccgct tgttcttgac ggcaatcttc
      421 tcgccttggg aatactgcga cagggccagg atgtgaatgt tgggctctac tttggggagg
      481 tctctcaggg taaagattat caactccgtc ttctcctgcg gactcatctt tgccattccc
      541 ctgatcaact gaaggtagtc gatgatgacg agaagcttat cgtcgggcct aggagccagc
      601 ttcctgcgca tcatccggat tcgtgccctc agcttatcaa ttcgcagcgg agttgtgtca
      661 tcgatcttta ttggaagccg cgaaagttct ttggagacct cgtgcattgc tggtatatga
      721 gtgtgctgat tcatcaaccg tggatcgcgc atttgtgcgg ccgtcaactt gtcactcatc
      781 gacgcataga accgtcgagc taactgctcc tttgtcattt ccagtgagaa ccacagcacc
      841 ggagtaccct cgcgagcatt ggctattgtc acctgaactg caaaggccgt ctttcctccc
      901 gaactctctc cagaaagtac cgagaactca ccgcggaagc atccccgagt ttccgcatcc
      961 atcttcggat ggccccaggt catttctagg gccgttcggt catccgttat ctgccgggcc
     1021 ttcaacacgc tggattctac agccggagtt atagatccga tgtctaccgc attgttaatt
     1081 ccctcgccta cgatctcagc caagtcaact tggaccgagc caagcacctc gatagcaggc
     1141 atactctgat ccgctgcacg agccgcccca accgtacata cggacatgat ccttcgcagt
     1201 agagccttgt ccttgatgat ccgaacgtac tcagtgataa caggccgacg tgggagccct
     1261 tccgttagcc ccgctaagta tgcgactccg cctacggatt ccacccagtt tctttttctc
     1321 aattcgttcg caagcgtaac gatatcgcaa ctcttggcgc cctcgatcat gccgaagagg
     1381 atctcggtca tcacgatgta tattcttcgg tgggaatcaa gatggaagtc atccgactcg
     1441 attattgccg tgtcgtcgaa gaaagcctcg ttgtcgttca agatcgcgcc aagcacggtc
     1501 ctttcgcaat ctatagcggc gggaagtcct gtatcgatgt ctctgatttc catttgtact
     1561 ttcggagttg gtttctcagg aaagctaccg actcatttcg cctcctctgg aattt
//
LOCUS       DATMKH010000031         1522 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2264817, whole genome shotgun sequence.
ACCESSION   DATMKH010000031 DATMKH010000000
VERSION     DATMKH010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2264817"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..788
                     /locus_tag="VNV63_00325"
     CDS             <1..788
                     /locus_tag="VNV63_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arabinose isomerase"
                     /protein_id="HXC61092.1"
                     /translation="RVEQVKDDEVQAVLNCTRGLFEISSDSPSDPLAKAPKPEALNWS
                     AKVAAGLDRLVADFRLDSLSYYYRGTDANPQERLAAGLILGNTLLTGRGIPCSGEGDM
                     KNAIAMLIMDRLKAGGSFTEFYAMDFVDQFLLMGHDGPFHLAIADGKPALRGLGLFHG
                     KRGQGISVEARVKTGPVTILGVTQTREGNLKFLVAEGDSIPGPILRIGNTNSRIRFPI
                     RPAEFMDAWCAEGPTHHCALGVGHVAKDIGKVARILGLKIVRI"
     gene            complement(1236..>1522)
                     /locus_tag="VNV63_00330"
     CDS             complement(1236..>1522)
                     /locus_tag="VNV63_00330"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HXC61093.1"
                     /translation="FAALVVKVQKERERKVITTGPYRYVRHPMYAGMIFYLIGAPLLL
                     GSWWGLLWGSGLLGLFSIRLLIEEGTLRKGLPGYNDYAARVRYRLVPGVW"
BASE COUNT          364 a          378 c          422 g          358 t
ORIGIN      
        1 cccgggtcga gcaagtcaag gatgatgaag ttcaagcagt cctcaactgc acccgtggct
       61 tgtttgaaat tagtagcgac agtccgtccg atcccttggc gaaggctcct aagcccgagg
      121 cgttaaactg gagtgcaaaa gtcgcggccg gcttggatcg tctcgtggcg gattttcgct
      181 tggatagttt atcctactat tatcgcggaa ccgatgctaa cccgcaggag cgattagcgg
      241 cgggactaat ccttggaaac acattgctaa cagggcgggg aattccctgc agcggggagg
      301 gagatatgaa gaacgcgata gctatgttga ttatggaccg cctcaaagcc ggcggatcct
      361 tcactgagtt ttatgcgatg gatttcgtcg accagttcct actgatggga catgatggtc
      421 ccttccatct ggctatcgct gacggaaaac ctgccttacg cggactcgga ctctttcacg
      481 ggaaacgcgg ccagggaatc tcggtggaag cacgggtcaa aaccgggccg gttacaattc
      541 ttggcgtcac ccagacgaga gaaggtaatc tgaagtttct ggttgctgaa ggcgactcta
      601 tccctggtcc gattctgcgg atcggtaaca ccaattcccg gatccgattc ccgattcgtc
      661 cggccgaatt catggacgct tggtgcgcag aaggcccaac ccaccattgc gcactgggag
      721 tcggtcacgt cgcgaaagac attgggaaag tcgccaggat cctagggctg aagatcgtcc
      781 ggatataatg atcgcgttta cgcggcaagt tcccaattaa ccggtgatcg tagaccgaga
      841 cttggtggga gggtggaatg ggccggcgtt agtcttgcgg tttaaggccc ctagcggttt
      901 gcttggcttc tgcttggtct tctgatactt cgaatactgt gcccatccat ggatagatag
      961 tgtattggaa gtaaactgtt cgaccgggat taatgtggac cttttgagta tctggtccga
     1021 aattaagcgt gatatcaaaa gggtgtctta ggatgtgatc accggccgca agctcaaatt
     1081 gcatgtaacg accgtttttg agtttgtgcc tcgggccatt atcgatgctg aacgaatagg
     1141 ttcgcccaag tttcctgagg ccagtgtcaa aagacgtgct ggacgagtcc aagagtatcg
     1201 caaatctgct cgaatcaaac ttttccggat ctggtttacc agacacccgg aactaagcga
     1261 taccgtactc gggccgcgta gtcattataa cccggcagcc ctttcctaag ggttccttcc
     1321 tcgatcagaa gacgaattga gaaaagaccg agtagtccgg atccccagag aagaccccac
     1381 caggagccga gcagcagagg cgcaccaatt aaatagaaga tcatgccggc atacatcgga
     1441 tggcggacat atcgataagg tccagtggta atgacctttc gctcacgttc tttctgaact
     1501 tttaccacca gagctgcaaa cg
//
LOCUS       DATMKH010000032         1460 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_340077,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000032 DATMKH010000000
VERSION     DATMKH010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340077"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="VNV63_00335"
     CDS             complement(<1..489)
                     /locus_tag="VNV63_00335"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXC61094.1"
                     /translation="MIWMVLVKLILTLGFLAVVFTGFLLILNSHPPRYPIENPPSHYG
                     LRFEDVTFPSLDGVELKGWFLQGGTDRQGAERRRPAIVICHGLGASKADFTGLAADLV
                     QAGFHVLLFDFRAHGKSKGFRTSFGYLEQRDLQGALRFLNGRPEVDPERIGAYGFSMG
                     GSV"
     gene            complement(697..981)
                     /locus_tag="VNV63_00340"
     CDS             complement(697..981)
                     /locus_tag="VNV63_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61095.1"
                     /translation="MKPNDSSEHPPRMIEITILGKPYKVEEDYLLWVFQKLGMVRFYS
                     QFCWNAECNNCPVTFKLNPQDPDQTKKACRTMACPGMIVTAMPEEFYKMR"
     gene            complement(993..>1460)
                     /gene="nth"
                     /locus_tag="VNV63_00345"
     CDS             complement(993..>1460)
                     /gene="nth"
                     /locus_tag="VNV63_00345"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HXC61096.1"
                     /translation="ATTPERMLKLSTRQIEKAIYPVGFYRTKARNIKSICQVLITQYH
                     SKVPDEIDELLKLKGVGRKTANLVVTLGYHKPGICVDTHVHRISNRLGYIKTKTPEQT
                     ETVLREKLPRKYWMIWNDLLVTYGQNLCRPISPLCSQCRLTPYCSRVGVKQSR"
BASE COUNT          354 a          390 c          361 g          355 t
ORIGIN      
        1 cacagagcca cccatcgaga aaccataggc accgatgcgt tccggatcca cttcgggcct
       61 tccattaaga aagcgcaaag ccccttgcaa atcccgttgc tccagatagc caaaggaggt
      121 ccggaaacct ttgctcttcc catgggcccg aaaatcaaac agcaggacat gaaaaccggc
      181 ctgcacaaga tcggctgcca atcctgtaaa atcggccttg ctcgctccaa gcccgtgaca
      241 gatcacaatg gcgggccgac gccgctccgc tccttgacga tctgtccccc cttgaaggaa
      301 ccaacccttg agttctaccc catcgaggct gggaaaagtt acatcttcaa accggagccc
      361 gtaatgagaa ggagggttct cgatgggata ccgcggcggg tgactattga gaattaataa
      421 aaaaccggtg aagacgacag ccaggaaccc caaggtcaaa atcagcttga ccaataccat
      481 ccagatcatg atgatgggta ctctaacgga tttagaacga tctgtcaaac catctaaaga
      541 cataaggcgt aaggatgccc gagctcttct acacaattgc gccatcatca ttctctgcaa
      601 tcgggcttgc cgctaccgct aaaggacttt tgaagatggc gatctgcgtt cgatctgaat
      661 ccagttttgt cggtcagctt aagagcccgc aggctcttag cgcattttgt agaactcttc
      721 cggcatcgcc gtgacgatca tgccgggaca cgccatggtg cggcaggcct tcttggtctg
      781 atcaggatct tgaggattca acttaaaagt caccggacaa ttgttgcatt cggcgttcca
      841 gcaaaattgg ctgtagaatc gaaccatccc gagcttttgg aatacccaga gcagatagtc
      901 ctcctcgact ttgtaaggtt tcccaaggat cgtgatttcg atcatccggg gcgggtgttc
      961 tgatgaatcg ttcggtttca tttaatttat ccctatcggc tctgtttgac acccacgcgg
     1021 ctgcaatatg gagtcagccg gcactggctg caaagcggtg agatgggcct gcagaggttt
     1081 tgtccataag tcaccaagag gtcgttccag atcatccagt attttcgagg cagcttttcg
     1141 cgcaacaccg tttcggtctg ctccggcgtt ttggtcttga tataccccag ccgattggag
     1201 atccgatgga catgggtgtc tacacagatc ccaggtttgt gataacctaa tgtcaccaca
     1261 agattggcgg tcttccgtcc tactcctttg agcttcaaga gttcatcaat ttcatccggc
     1321 accttggaat gatattgggt gatcaacacc tgacagatgg attttatatt ccgggccttg
     1381 gttcgataga aaccgacagg gtagatcgcc ttctcgatct gacgggtgga gagtttcagc
     1441 atcctttccg gcgtcgtggc
//
LOCUS       DATMKH010000033         1423 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1623135, whole genome shotgun sequence.
ACCESSION   DATMKH010000033 DATMKH010000000
VERSION     DATMKH010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1623135"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..1142)
                     /gene="yvcK"
                     /locus_tag="VNV63_00350"
     CDS             complement(45..1142)
                     /gene="yvcK"
                     /locus_tag="VNV63_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HXC61097.1"
                     /translation="MGEQVHCGKIADLGSYRLRRIPKKQVLSPDAHLSTLRVVALGGG
                     TGLPILLCGLKSALFPPERAWELSRDLDRLTAIVTVADDGGSSGRLRRAYPMLSPGDI
                     RNCLLALSNGDPTLAAVFNFRFNGHGDVAGHNLGNLILTALSELEGNFFKAIEWGSKI
                     LEIRGKVLPCTLDTVDLLAEFSDGSYVEGESQIATIHRPIRRVRIHPEYAQAFPEARA
                     AILAADLIVIGPGSLYTSLIPNLLIKGIANAIADSKARVILVMNLMTEPGETDDYTAA
                     DHLQALRLHAPQVRIHDVLLNNGPIPDRLTKRYAAEGSRPVFPEVEFLKTLGCRPVVR
                     DFLGVGPKLHHDSHKLAQAILTLTTDEQTGD"
     gene            complement(1112..>1423)
                     /locus_tag="VNV63_00355"
     CDS             complement(1112..>1423)
                     /locus_tag="VNV63_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014786326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HXC61098.1"
                     /translation="YSGEAIPYYSLRCVIRPGVTGWAQVRYGYANDLEEEIEKMRYDL
                     YYIKHMSLGLDLRIFLDTIKVALLGQGSEAANTAQHLPGSQDAVKSGRRSWESKSTVE
                     K"
BASE COUNT          331 a          400 c          380 g          312 t
ORIGIN      
        1 tttgaccggc aaagacaggc cgccaccatt ctgtacgaaa gctgtcaatc gccggtctgt
       61 tcatcggtgg tcagcgtaag aatggcctgg gcaagtttgt gggaatcgtg atgaagcttg
      121 ggcccaaccc caaggaaatc ccgcactacc ggccgacaac ccaaggtctt taagaactcg
      181 acctctggaa agacgggacg ggaaccttca gctgcgtagc gcttcgtcag ccgatcaggg
      241 atgggtccat tattcaggag tacgtcatgg attcgcacct ggggcgcatg gagacgaagg
      301 gcctgcaggt ggtcggccgc ggtatagtca tcggtttcgc cgggctcggt catcagattc
      361 atgaccagaa tgactctggc cttcgagtcc gcgatggcat tggcgatccc tttgatcaac
      421 aggttcggga tgagactcgt atagagactt ccaggaccga tgacgatcag gtccgccgcc
      481 agaatagctg cccgggcttc ggggaaagct tgagcgtatt ctggatgaat ccgcacgcgc
      541 cggatgggac ggtgtatcgt ggcaatttgt gattcgcctt ccacataact cccgtcagag
      601 aactcggcca gcagatcgac ggtatcaaga gtacagggaa gcaccttccc ccgaatctcc
      661 aaaatcttac tcccccattc tatcgcctta aaaaagttcc cctctaattc actgagagct
      721 gtcaggatga gatttcctag attgtgcccc gcaacatcac cgtgaccgtt aaatctgaag
      781 ttgaagaccg cggccagtgt gggatcgcca ttcgagaggg caagaagaca attccggatg
      841 tctcctggag agagcatcgg ataggcccgt cggaggcgtc cagaacttcc accgtcgtcc
      901 gccactgtaa caattgccgt gagacggtcc agatccctgg acaactccca cgcccgctcg
      961 ggtggaaaga gggcagactt cagaccgcaa agaaggatgg gaagaccggt cccgccgccc
     1021 agcgcgacga cccggagggt tgaaaggtgg gcgtctggac ttagaacctg cttcttcggt
     1081 atcctccgca agcgatacga tcccaaatcg gctatttttc cacagtggac ttgctctccc
     1141 acgatctcct ccctgatttt acggcatcct ggcttccagg aaggtgctgt gccgtattgg
     1201 ccgcttccga cccttgaccc aacaaggcga ccttgattgt atcgaggaat attctaagat
     1261 ctaatccaag tgacatatgc ttaatataat acaggtcata tctcatcttt tcgatttctt
     1321 cttcaagatc gtttgcatag ccgtatcgta cctgcgccca gccggtgact cccggccgaa
     1381 tcacacaacg aagcgagtaa tacggaatcg cctcgccact gta
//
LOCUS       DATMKH010000034        10845 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3868880, whole genome shotgun sequence.
ACCESSION   DATMKH010000034 DATMKH010000000
VERSION     DATMKH010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 10845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10845
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3868880"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..884
                     /locus_tag="VNV63_00360"
     CDS             <1..884
                     /locus_tag="VNV63_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012900136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HXC61099.1"
                     /translation="LRLQDRAAQQMKTLRHVHGALSLETIEARLVFDDGKLKDVEAEK
                     KNRAKDIIEDFMIAANGVTARFLASKNLPSILRVVRTPKRWDRIVELALEQRSTLPKE
                     PDSKALELFLIQARASDPLRFPDLSLSIIKLLGAGEYVVEHPGDKSGGHFGLAVRDYT
                     HSTAPNRRYPDIITQRLLKTAMDGRSLPYKNEELEALAKHCTEKEEAAKKVERQVRKS
                     AAAMLLEHRIGERFDAIVTGTGEKDTWVRLLFTQIEGRLASGFEGMDVGDRLRVRLIS
                     TDMARGFIDFEGVKGET"
     gene            881..1447
                     /locus_tag="VNV63_00365"
     CDS             881..1447
                     /locus_tag="VNV63_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61100.1"
                     /translation="MKLKIGVMGSATGNLSKRQKEIAYELGSAIAENGCVTITGACPG
                     LPLESAKGAAKAGGLVVGISPALSEWEHVNKYHSPLEYHDVLIFTGSGLMGREVVNIR
                     SSDIVVIIGGRSGTLGEFAIAYDEGRLIGVLRGTGGITSAIKSIVKIVKKKTGAKIIY
                     GKSPRTLVKNLIDYFTKGHNRHPRIHQV"
     gene            1495..2775
                     /locus_tag="VNV63_00370"
     CDS             1495..2775
                     /locus_tag="VNV63_00370"
                     /inference="COORDINATES: protein motif:HMM:NF015451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HXC61101.1"
                     /translation="MDRGGGGAKAKTKTDDLWDTVVIGGGPAGMMAAGRAAERGRSVL
                     LLEKNPTLGKKLLITGGGRCNVTNNKPEIHTILTEYKNNNKFLFSAFSQFNVEDTLNF
                     FNQRGMATKVEAEGRVFPASNKAQSVWDVLVRYIKDGGVQIQTSAAVTGLFLDIATKC
                     ISIRLNDKTEIKAKSCIIATGGTSRPETGSTGEGFMWLKKIGHTIIDNNFALVPIALK
                     DAWAKRLGGVTLADIKLTTFQNGEKQEVHKGKLLFTHFGISGPTVLNMSKEIGELLQY
                     GEVIITLDLLPKLDHGALKQELQTLLIAESNKKLKNTLGKLIPSALVAAILELADING
                     ETANHSVRRENRIKLVALIKAIPLNVKSLLGADKAVVSSGGVAPEEVNFKTMQSRLVP
                     NLYIIGDVLNIDRPSGGYSLQLCWTTGFVAGSSS"
     gene            2884..3573
                     /locus_tag="VNV63_00375"
     CDS             2884..3573
                     /locus_tag="VNV63_00375"
                     /inference="COORDINATES: protein motif:HMM:NF016163.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HXC61102.1"
                     /translation="MPSVMGLDGYHGGWVGVWIEYQNGKDGSFKTRTFGSFKDVLALN
                     PFPDVIGIDIPIGLLDKQEKGGRVCDREARRMLGSPRSSSVFSPPVRRALGATVYEEA
                     KRHGLTLQGFGILPKVREVDEQMTPELQKKIFEVHPEVSFRAMNGGPMRHNKKGSQGR
                     SDRIQALECKLPGFTRQHLEERPEGASEDDLLDAYAAAWTAMRIVAFKAIRFPQNPPK
                     DQKGLRMEIWY"
     gene            4197..4778
                     /locus_tag="VNV63_00380"
     CDS             4197..4778
                     /locus_tag="VNV63_00380"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HXC61103.1"
                     /translation="MLFKKHRITLATFLMIGFISLFIGLTAFSAKADDIPNTGLSLGG
                     RGAYYDPSDGNASWYGGAQLRLHLGTVFAVEGSVDYRRSKPDPSTRVDTYPVMASALL
                     YLFPGRISPFILGGAGWYFTHVDGPGGDNTQQRFGLHAGGGLEVYLNQYWSIDGTYRY
                     VWLEKIKSKDAALLDKDFNDDGHMVTVGLNYHF"
     gene            4873..5022
                     /locus_tag="VNV63_00385"
     CDS             4873..5022
                     /locus_tag="VNV63_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008242693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HXC61104.1"
                     /translation="MLWTIAVILIVLWAMGLVSSYTMGGFIHILLVLAIITVLVSIIQ
                     GRRVA"
     gene            complement(5052..5495)
                     /locus_tag="VNV63_00390"
     CDS             complement(5052..5495)
                     /locus_tag="VNV63_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61105.1"
                     /translation="MGKLIGVILILAIGFGAGYYVGTNRMVKLRKDVEKLKVEMEDKV
                     ARFEQQLTNLRHRDHLIVAKDKLHEAQRDLTERNFGDAQRAIQNAQEEIREAEKLAGP
                     SEKEKYSALSAGLNEISNDLTHPTRPQFREKIQRAAKDLDQLAGK"
     gene            complement(5504..6634)
                     /locus_tag="VNV63_00395"
     CDS             complement(5504..6634)
                     /locus_tag="VNV63_00395"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HXC61106.1"
                     /translation="MVLTIASFTIAGLDILNAEGLKTEKLKLACLKILTEALHNQLFN
                     VRSAAAKALGESRDEAAVPLLATALSDQDSMVRSFSVEALGGIHSKDAIRVLTRGLKD
                     SDPLIRGLTLKALGDQDDAALDPLLRQGMNDPDPGVRFAAAAAVAKHGDQKAIETVIQ
                     ALHSSQPQNRAVAAGFLAGSKEAEIFHALTQALGDADAEVRLTAAVALGKNGDPNSLE
                     PLLKRLQDTDPSVRAAVTEAIGELKQPTAIPFLKEAMKDPDPLVRISAGQGLALLSVT
                     DGRRYLKTALSDNDYNARASAARALGRIRDQDALPLLANAFRDPVSRVRIATARALGM
                     IGGTKSVEILRTGLSDPDLSVQSYAAGNILLLLPKTNNRKKE"
     gene            complement(6652..8310)
                     /locus_tag="VNV63_00400"
     CDS             complement(6652..8310)
                     /locus_tag="VNV63_00400"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HXC61107.1"
                     /translation="MRFNATLLWRVIRILFLAFTILFSYRVHSFLGLFQSEKNHRDRL
                     KRIHRRAAVRLRKTAIELKGVLIKLGQFLSARVDLLPEEYIEELSQLQDQIPPVEFHS
                     IRARIIEELGQEPEACFDRFEEVPLAAASLGQVHEAYLKTEKGPTQRVAVKVQYPGIQ
                     DIVETDLRAAKWTARLLTSRFHTIRFDILYEEFSRILHEELNYIHEGRNAERFHENFS
                     GDHAIVIPRVIWAFTTPHVLTLEFVEGIKISHLEELKARGVQLKEVAHLLMRSYVKQI
                     FEHRFLHGDPHPGNLFIQVQSDNDLKLVFVDFGLMQRITREIQDGMKTIVEAAIDRDQ
                     NGMVKGLLALGFIARGGNLKEVEKVVEFFMERYRDISPKELKRITIGQVREDLAQIFE
                     ISSSIQLPNNFLLIGRVVGMLNGLTARLDPDMNIIELAAPSVREFFRGREEGWLRKFL
                     AQGKAAGRSLFSLPGAVEEFFDLANRGQFQTRMSSEDVTGVLTKIYKLIYRVMMAFMT
                     TLLAFVWLELDRNGPKELIWLAGLITIGFGLALIWTFLKDLRRW"
     gene            8434..10188
                     /locus_tag="VNV63_00405"
     CDS             8434..10188
                     /locus_tag="VNV63_00405"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXC61108.1"
                     /translation="MTKKIKCERFTGMYERFFNFTETPFNLTPDPRFFFSSPKHEDAF
                     SQILYGIQERKGFIVLTGEVGTGKTTLCRLLLDRLDAKIKTSLIFNPNLTTVELLQAI
                     NQDFGIASKSQSKRELLDELNRFLLSGLSQRGNAVLIVDEAQNLTIDCLEEIRLLSNL
                     ETPKEKLLQILLVGQPELRDKLVLQELRQVNQRVSLRYHLDPLDLTETENYIGYRLKV
                     AGDREGIRFTPRAIDMIHRLTHGIPRLINLLCDKALLAAYVAETKVINATLVDRAQIE
                     LEGKPSKAPIASSSKRAQLDPAVIRPIAWRGGTVAALLLLGVLLWWWKVPGTPTSSLP
                     EPNAQAHMIAESGPVVPPSGDAKTKNLDPARSEFGMDADGIFRISDPKQADIAAYLTL
                     LSQWEIEPEIQEEDLSGLVPEVLIQQAGLKSYTVPLDWQKLRLLDYPALIQINSNGAA
                     PPHEAVLVGLSADGALVLDPLDGKWTPRREELERRWTGQAVLLWKELDGISLPVNKED
                     AGSTSVKNLQRVLSTEGFYLGKVDGAFGPATHLAMKFFQQKMGLKDDGVLDLESYLVL
                     AKITQQEDVPNLHLLNAE"
     gene            10354..>10845
                     /locus_tag="VNV63_00410"
     CDS             10354..>10845
                     /locus_tag="VNV63_00410"
                     /inference="COORDINATES: protein motif:HMM:NF022800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HXC61109.1"
                     /translation="MKLKMQQITSRFFAPLLIILITLAAWFLADLINLVLGGQFTVPT
                     SKALASTGIATTQTEPGRRDLASVILERNIFNSRPSQSASIETAVTAVTEMTDLKLRL
                     IGTVVGGVQSSFAIIEDQSKKEQQLYRLDDLVAQGAKLVKIERNEVVLQRGSIKETIS
                     LYPE"
BASE COUNT         2648 a         2715 c         2954 g         2528 t
ORIGIN      
        1 atctccggct ccaggaccgg gcggcacaac agatgaagac gctccggcac gtacacggtg
       61 cactctctct cgagacaata gaagcgcgcc tggtttttga cgacggcaag cttaaggatg
      121 ttgaagccga aaaaaagaac agggctaaag acattattga agacttcatg atcgcggcaa
      181 acggtgttac cgcgcgtttt cttgcttcca aaaacctgcc ttcaattctg cgtgtggtcc
      241 ggacgcccaa acgatgggac cggatcgtcg aacttgcatt ggaacagcgt tctacactgc
      301 ccaaggagcc tgattcaaag gctttggagc tgttccttat acaggcgaga gcttccgacc
      361 cgctccgctt cccggatctt tccctttcta ttatcaagct gctgggtgcg ggtgaatacg
      421 ttgtcgaaca tccgggggat aaatcaggcg gtcatttcgg tcttgcggtt agggactata
      481 cccactccac agctccaaac cggcgctacc cggacattat cacgcagcga cttttgaaga
      541 cagccatgga cggacgctcc ctaccctata agaatgaaga gctggaagcg cttgcaaaac
      601 actgcacgga aaaagaggaa gcagccaaaa aggtcgaacg gcaagtcaga aaatccgcgg
      661 cagcaatgct gctggaacat cggatcgggg aacggttcga tgccatcgtc acgggcacag
      721 gcgaaaaaga cacctgggtc cgtcttctgt ttacgcagat tgaaggaagg ctggcgagcg
      781 gttttgaagg gatggatgtc ggtgacaggc tgcgggtacg gctgatcagc acggacatgg
      841 cgcgcggatt tattgacttc gagggagtga aaggggaaac atgaaactaa aaattggtgt
      901 tatgggttcc gcaaccggca acctttccaa acggcaaaag gagatcgctt atgagctggg
      961 ctcggccatt gctgaaaacg gctgtgtaac gattaccggg gcctgtccgg gattgccatt
     1021 ggaatcggcg aagggagcag ccaaggcagg cggtctagtc gtaggtattt cgccagcctt
     1081 aagcgaatgg gaacatgtaa acaaatatca ttcgccattg gagtatcatg atgtactaat
     1141 ctttacggga agcggactta tgggaagaga agttgtaaac atacgaagct ccgacattgt
     1201 tgtcatcatc ggcggccgtt cagggacact cggagagttt gcaattgctt atgacgaagg
     1261 caggttaatt ggtgttcttc gcggcaccgg cggcattacc tctgcaataa aaagtatcgt
     1321 caaaatcgtt aagaagaaaa cgggtgccaa aataatctat ggcaaaagcc cgcggaccct
     1381 ggttaaaaac ctcattgatt acttcacgaa ggggcataac agacatccac gtatacatca
     1441 ggtctagaac tcggttatcg gaagaataag acttcaaaat caggagaaag acaggtggac
     1501 cgagggggcg gcggagcgaa agctaaaaca aagacagatg acctatggga caccgtcgtt
     1561 atcggcggcg ggccggccgg gatgatggcg gcgggcaggg cggctgagcg tggccgctcg
     1621 gttttgctcc tcgagaaaaa tccaacactt ggcaaaaagc ttctcattac gggtggcgga
     1681 cggtgcaatg taactaataa taagcccgag atccatacga tactcacaga gtataaaaac
     1741 aataataaat ttctcttctc cgctttttca cagtttaacg tcgaggacac gcttaatttt
     1801 ttcaatcagc gcgggatggc gaccaaagta gaagcggagg ggagggtttt tcccgcttca
     1861 aataaggcgc agtcggtttg ggatgtgctc gttcgctata ttaaagacgg cggtgtgcag
     1921 atacaaacca gtgccgccgt gaccggcctt tttttagata tagcgaccaa gtgcatcagt
     1981 atccggctta atgacaaaac agaaataaag gcaaaatcgt gcattattgc taccgggggt
     2041 acttcacgcc cggaaaccgg ttcaaccggg gagggattta tgtggcttaa aaaaatcggc
     2101 cacacgatca tcgataataa ttttgcgctt gtacccattg cgctcaaaga tgcatgggca
     2161 aaaagattag ggggagtaac acttgccgat attaaactca ccacgtttca aaatggggaa
     2221 aagcaagagg ttcacaaagg caagctgctc tttacgcact ttggcatcag cgggccgacc
     2281 gtcttgaata tgagcaaaga gatcggtgag cttttgcagt acggcgaagt tattatcacg
     2341 ctcgatctac ttccaaaact cgatcacggt gcgctcaagc aagagctcca gacgctcctc
     2401 attgccgaga gtaataagaa attgaaaaat acgctgggta aacttattcc ttcggcactt
     2461 gttgcggcga tactcgagct tgccgatata aacggcgaga cggctaacca tagtgtgagg
     2521 agagagaatc gaataaaatt agttgcactg attaaagcca tcccgctcaa cgtcaaaagt
     2581 ttgctgggtg cggataaagc ggttgtatca tcgggtggcg tggcacccga agaagtaaac
     2641 tttaaaacaa tgcagtcgcg cctcgttcct aatctgtata tcatcggcga tgtcttaaac
     2701 atcgaccgtc cctcgggtgg ctatagcctc cagctctgtt ggacgaccgg ctttgtagcg
     2761 gggagcagca gctaaagcaa gaggcgcttt gtgcaattcg atacgggcat tctgccggcc
     2821 cgataatagc acccgacctg ggagccgacc gggaaggaaa aaagtgaagg agagggtgca
     2881 accatgccaa gtgtgatggg actggacgga tatcatggag gatgggttgg cgtatggatc
     2941 gaatatcaaa acggaaaaga cgggtccttt aaaacgagaa cgtttggttc gttcaaggat
     3001 gttcttgcgc tcaatccgtt cccggatgtg atcgggatcg atatcccgat cggtctgttg
     3061 gacaaacaag aaaagggcgg gcgggtgtgt gatcgggagg ccagacgaat gctcggttcg
     3121 ccacgatcga gcagtgtgtt ttctccaccc gttcgcaggg cgctcggggc caccgtctat
     3181 gaagaagcga agcggcatgg tctgactctg caaggattcg gaatcttgcc caaggtccgc
     3241 gaagtggatg agcagatgac tcccgagttg caaaagaaaa tctttgaggt gcatccggag
     3301 gtatcgtttc gggcgatgaa cggcgggccg atgcggcata acaagaaagg ttcccaaggt
     3361 cgatcggatc ggatccaagc tcttgaatgt aagctccccg gttttaccag gcagcatctt
     3421 gaagaaagac cggagggagc tagtgaggac gatcttttgg acgcgtacgc cgcggcctgg
     3481 acggcgatgc ggatcgtcgc tttcaaagcg atccgttttc ctcagaatcc gcccaaggat
     3541 caaaagggtc ttcgaatgga gatctggtat tagatgagag acggttcgcc ggctggaatg
     3601 acggctacac tcgagtaaca atcggatcaa aaaaaaggga tagaacgcga aaaacgcccg
     3661 atcccttttt ttgcaagagt tgcggcttac ttctttttct tcttagccgc tttctttttt
     3721 ttcaccacga tcgcatcacc ccctttatga tataagcata tatcacgaaa tttcaaaagg
     3781 agcaagttaa atatttgcat gtatttttct gagtggtact ttatgttgtc ttcggcactc
     3841 aagaaagccg agtaattccg ccatcgccaa ttccgaaatc ttaaaaaaac ctggagctaa
     3901 tgaagacaca ataggaaaca ctccaacccg tcccggatgc gagcgggtgc aaagagatct
     3961 gtcctgagcg cgattggcgg ccccgggatc aggatttaaa atccttccgt tctttttttt
     4021 aaaacctcgg gctgggatcc ggccgttatg gatcttgatc cctccagcct taattctcat
     4081 gtatgcggtt ctttcgggat tttcactaag agttatacag gttttacctg attttcatcc
     4141 ggcggttcat tgcctatgat ggtatcaaca gaaactataa aaaaggaggg atcatgatgc
     4201 tgttcaaaaa acataggatt acacttgcga catttctgat gataggattc atatcgctat
     4261 ttatcggcct gacagccttc tctgcaaagg ccgacgatat accgaatact ggattgtcct
     4321 tgggtggtcg gggagcttac tatgacccga gcgacgggaa cgccagctgg tatggcggcg
     4381 cgcaactcag actgcatttg ggcactgtgt ttgcagtcga aggatctgtt gattatcgca
     4441 ggagcaaacc cgatccttca accagagttg atacctaccc ggtgatggct tcggcgttgt
     4501 tatatctttt cccagggagg atcagtccct ttattctagg aggggcggga tggtacttca
     4561 cacatgtcga tggtcccggg ggagataata ctcaacagcg cttcggcttg catgccggtg
     4621 gcggattaga ggtctatctc aaccagtatt ggtcgattga tgggacctat cgctacgttt
     4681 ggttagagaa aataaaatcg aaagatgctg ctcttctcga caaggacttt aacgacgatg
     4741 gccatatggt aactgttggc ctcaattacc atttctaagt ccaagaacaa taaaaaaggt
     4801 ggtcattgct ggcgggtaag ggaatgcccg ccggcaatga gcctgagcaa tcgaaaagga
     4861 ggatatgata taatgttgtg gacaattgct gtaatactaa tagttttgtg ggcgatggga
     4921 ttggtgagca gttatacgat gggagggttt attcacatcc tgttggtgct tgcgattatc
     4981 accgtcctgg tcagtatcat tcaggggcgg agagtcgcgt aacgacgata attagttagg
     5041 aggccaatcc attattttcc cgcgagctgg tccagatctt ttgcggcccg ttgtatcttc
     5101 tcgcggaact ggggacgggt gggatgcgtc aggtcattgc tgatctcatt caggccggcg
     5161 gaaagcgcgc tgtacttttc tttctccgaa ggaccggcca gcttctccgc ctctcggatt
     5221 tcttcctgcg cgttctgaat cgccctctgg gcatcgccga aattgcgctc ggtcagatcc
     5281 ctttgggcct cgtgcagttt gtcttttgcc acgattaggt ggtcccgatg gcgcaggttc
     5341 gtcagttgct gctcaaatcg ggcgaccttg tcctccatct caacctttag cttttcgaca
     5401 tcttttctta attttaccat gcgatttgtg cccacataat atccggctcc aaacccgatt
     5461 gccaagatca agatcactcc gatgagcttt cccatagtcg tgttcactcc ttctttcgat
     5521 tatttgtttt tggcaaaagc aacagtatgt ttccggcggc gtagctttga accgagagat
     5581 ccgggtcaga gaggcccgtc cgcagaattt ctacagactt cgttccgccg atcattccca
     5641 aagcccgcgc tgtggcgatg cgtacacggg agaccgggtc ccgaaaggca ttggccagaa
     5701 gcggcaacgc gtcctgatcg cgtatacgac ccagggcccg agcggccgag gcacgtgcat
     5761 tatagtcgtt atcgctcaag gctgttttca gataacgacg cccgtctgtg accgaaagta
     5821 aagccaggcc ttggcccgcc gagatccgga ccagcgggtc gggatctttc atcgcctcct
     5881 tcaaaaacgg aatcgccgtt ggttgtttga gctctcctat tgcctcggtc actgccgccc
     5941 gtacggatgg atcagtgtct tgaaggcgct tgagaagcgg ttcaagactg tttggatcgc
     6001 cgtttttacc gagcgcgacc gcggcggtga gtcggacttc cgcatcggca tcgccgagcg
     6061 cctgagtgag cgcatgaaag atttcagcct ccttgctacc ggccaaaaaa ccggctgcga
     6121 ccgcccgatt ctgcggctgg gaactatgaa gcgcctggat cacggtttca atcgctttct
     6181 gatccccgtg tttggccacg gctgcagctg ccgcaaagcg gacgccggga tccggatcat
     6241 tcattccttg tctcagcaga gggtcaagcg ccgcatcgtc ctgatcaccc agggctttga
     6301 gcgtaaggcc ccggatcaaa ggatctgaat ctttcaaccc ccgggtcaag acccgaatcg
     6361 cgtccttgga atgtatcccc cccagcgctt ctaccgaaaa ggaccggacc atgctgtctt
     6421 gatccgacag cgcggtcgca agcagaggta cagccgcctc gtcccggctt tcacccaagg
     6481 ccttggcggc ggcgctacgg acattaaaaa gctggttgtg caaggcttcg gtgagtattt
     6541 taaggcaggc cagctttagc ttttctgttt tcaggccctc agcgtttaat atatcgaggc
     6601 cagcaatcgt gaaagaggcg atggtcagta ccatcacgac caatcgtttt atcaccatcg
     6661 ccggagatct ttcaagaagg tccagatcag tgccaggccg aagccgatgg tgatcaggcc
     6721 ggccagccag attaactctt tggggccgtt ccggtcgagc tcgagccaga caaaagccaa
     6781 aagcgtggtc atgaaggcca tcatgactcg gtaaatcagt ttataaatct tggtcagtac
     6841 gcctgtgacg tcctccgagg acatccgggt ctgaaactgc ccgcggttgg caagatcgaa
     6901 aaactcctcc accgcaccgg gcagagaaaa gagagagcgg cctgccgctt ttccctgagc
     6961 taaaaatttc cgcaaccagc cttcctctct tccgcgaaaa aattccctta cactgggagc
     7021 cgccagttcg atgatgttca tgtccggatc cagccgggcg gtcaatccgt tgagcattcc
     7081 cacgacccgt ccgatcagaa gaaagttgtt gggaagctgg atagaggacg agatctcgaa
     7141 gatctgggcc agatcttcac ggacctggcc gatcgtgatc cgcttgagtt ctttgggcga
     7201 gatgtcccga taccgttcca tgaagaactc gaccaccttc tccacttctt tcaaatttcc
     7261 tccccgggca ataaatccca aggcgagaag ccccttgacc atgccgttct gatcccgatc
     7321 aatcgccgcc tcgacgattg tcttcattcc gtcctgtatt tcacgggtga tccgctgcat
     7381 gaggccgaag tccacaaaga ccaacttcag gtcgttgtcg gattgcactt gaataaaaag
     7441 gtttcccgga tgaggatccc catggaggaa gcggtgttca aagatctgct tgacatacga
     7501 cctcatcagc agatgcgcaa cctctttgag ttgaacaccg cgggccttaa gctcttcgag
     7561 atgactgatc ttgatccctt ccacaaactc cagcgtcagg acatgggggg tggtgaaagc
     7621 ccagatcacg cgggggatga caatcgcgtg atcaccggag aagttctcgt ggaaccgctc
     7681 cgcatttctt ccctcgtgaa tgtagttgag ttcttcatgc agaatgcgtg agaactcctc
     7741 gtagaggatg tcaaaccgga tcgtgtggaa gcgggaggtc aaaagccgcg cagtccattt
     7801 ggctgcacgg agatccgtct cgacgatgtc ttggataccc gggtactgaa ccttgaccgc
     7861 gacgcgttga gtcggaccct tttcggtctt cagataagcc tcgtggactt gtccgagaga
     7921 ggcggccgca agcgggactt cttcaaaccg atcaaagcag gcttcaggtt cctgccccag
     7981 ctcttcgatg atgcgtgccc ggatcgaatg aaattccacc ggcgggattt ggtcctgaag
     8041 ttgggaaagc tcttcgatat attcctcggg aaggagatcc acacgggcgc ttaggaactg
     8101 gccgagcttg atcaggacgc ccttgagctc gatcgctgtt tttcggaggc gaacggcggc
     8161 ccggcgatgg atccgcttca agcgatcacg atggttcttt tcggattgaa aaagtccaag
     8221 gaaggaatgg acccgataac tgaacaggat tgtgaaggcg agaaaaagga tccggatcac
     8281 tcgccaaaga agtgtcgcat tgaatcgcaa gaactctcct tttcaaaagg aacggccccc
     8341 atcttaccat catcaaagaa cgctttgtca agcgcggtcc tcgttcacaa ccgttgcacc
     8401 cttccctcat tgggatcacc gacgggatgc ctattgacaa agaagatcaa atgtgagaga
     8461 tttacgggga tgtacgaaag gttttttaat tttaccgaga ctccttttaa cctcacgccc
     8521 gatccacggt ttttcttctc cagccccaag catgaagatg ccttctctca aatcttatac
     8581 ggcattcagg aacggaaagg ctttatcgtc ctgaccgggg aggtggggac cgggaaaacg
     8641 accctctgca gactcctgct ggaccggttg gatgccaaga tcaagacctc actgatcttc
     8701 aatcccaacc tcaccacggt cgagctgctc caggcgatta atcaagactt cggaatcgcc
     8761 agcaagagcc agtccaagag agagttgctg gacgagttga atcgttttct gctgtcaggg
     8821 ttatctcaga gaggcaatgc ggttttgatc gtggatgagg cacaaaatct gacgatcgac
     8881 tgccttgaag agattcggct gctttccaac ctcgagacac ccaaggaaaa gctgctccag
     8941 attcttttag tgggccaacc cgaactgaga gacaaactgg tgctgcagga actccggcag
     9001 gtcaaccaac gcgtctcgct tcgttatcac ctggacccgt tggatctgac ggagaccgag
     9061 aactatatcg gttatcggct taaggtggcg ggtgatcggg aggggatcag atttaccccg
     9121 agagcgatcg atatgattca tcggttgaca catggcattc ctcgcttgat taatctactc
     9181 tgtgacaagg ccctgctggc tgcctatgtg gccgagacga aggtgatcaa tgcgaccttg
     9241 gttgaccggg cccagataga acttgaagga aagccgtcta aggccccgat tgcttcttca
     9301 tcgaagcgcg ctcagctcga tcccgccgta attcgtccga ttgcatggag aggcgggacg
     9361 gtggcggcct tactgcttct cggagtcctg ctgtggtggt ggaaggtccc tgggacgccg
     9421 acctcttcgc tgcctgaacc aaacgcccag gctcatatga ttgccgagtc cgggccggtc
     9481 gttccaccat ctggagatgc aaaaacaaaa aatttagacc ccgcccggtc cgagtttgga
     9541 atggacgccg acgggatctt tcgtatcagc gatccgaagc aggcggacat cgcggcctat
     9601 ctcaccttgt tgagccagtg ggaaattgag ccggagattc aggaggagga tttatccggt
     9661 ctggttccgg aggttttaat ccaacaggcg ggactcaaat cctacacggt ccctctcgat
     9721 tggcagaagc ttcggctttt agactatccg gcgctgatcc agattaattc caacggagcg
     9781 gccccgcccc atgaggccgt tctggtcggc ctctctgcgg atggggcatt ggtgctcgat
     9841 ccgcttgatg ggaaatggac gcctcgtcga gaggagttgg aacgtcgctg gaccggccag
     9901 gccgttctcc tctggaagga actggatggc atcagcctgc cggttaacaa agaagacgca
     9961 ggaagtacat cggtcaagaa tctccagcgg gtcttaagca cagagggctt ctatttgggc
    10021 aaggtggacg gtgcctttgg gcctgctacg catcttgcca tgaagttttt tcagcagaag
    10081 atgggcttga aagacgatgg tgtgttggat ctcgaaagct atctggttct ggccaagatt
    10141 acccagcagg aagatgtgcc caaccttcat ctcttaaatg cggagtagtt gagcaaagcc
    10201 gcttgatccc gctctgtaca gttgataaac ctgcgcagac tgcaggttta tcctgtcaag
    10261 gaagatagag tccgttttga atgcgttcgc cttgtggcga acgctcacag ttgaccgaat
    10321 cagataaaat cattatacta ttagataaca gccatgaaac tgaaaatgca acagatcacg
    10381 agccggtttt ttgcacccct gctcatcata ttgatcacac tggcggcctg gttcctggct
    10441 gatctgatca acctcgtcct gggaggtcag tttacggttc cgacctcgaa agcgctcgcc
    10501 tccacaggga tcgccacgac tcagacggaa ccggggagga gggatctagc ctccgtgatt
    10561 ctcgagcgaa acatatttaa ttcccgaccg agccagtcag cctctatcga gaccgcggtg
    10621 acagcggtga ccgagatgac ggatctcaag ctgcggttga tcgggactgt agttggggga
    10681 gttcaatcct cttttgccat cattgaagat cagagcaaaa aggagcaaca gctctatcgg
    10741 ctggatgatc tcgtggctca gggagcaaag ctggtgaaga tcgagcgcaa tgaggtcgtc
    10801 ctgcagcgcg gctcgattaa agaaaccatc tcgctctatc ctgaa
//
LOCUS       DATMKH010000035         1222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_341610,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000035 DATMKH010000000
VERSION     DATMKH010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341610"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            678..>1222
                     /locus_tag="VNV63_00415"
     CDS             678..>1222
                     /locus_tag="VNV63_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013582228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045735 - nutrient reservoir activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXC61110.1"
                     /translation="MALATAAFAGMTANAQEKSSTQKAEHDHSSSDPGPENKALLGEN
                     PNSNTPPPTDHGDVVPLWYSFDLVKKRVEQGGWTHEVTQSVLPSSRDIAGVNMRLTAG
                     SYREMHWHTADEWAYVLYGNARVTVMNPDGTMFIGDVGVGDLWLFPAGFPHSIQGLGP
                     DGTEFLLVFNQGNFSEDGTMLL"
BASE COUNT          295 a          331 c          319 g          277 t
ORIGIN      
        1 agacgtcagc attgtgcacc gggctttccc ggcggcaaag caacctttga gttggccgca
       61 atcgtttcca atctgacgga agccacggtg tgatcgtcac cggccggttg gagtccacaa
      121 tacggtctgc ctctcaaacc cgcatcgata atgccgaccg caggatccag ggaaccgaaa
      181 gtctttggtc aaattgcaag gtcaggcaaa tctccggatc tcaatctgct ttgattgtct
      241 gcaaatcgaa ctggtgcgtg ggttgattcg aagagtttcc cgcccgttga ctgaatatcc
      301 ccgagactag atagacaaat ccaaaacgat cttgaggtta atccagcgac agtttatgtc
      361 gaaatgtcga ctccaaaaga tggattagcc gattccagta tcttccttct ccgaagacac
      421 atcaccggaa tcccccgaga agcctagcga tttgctacac atatcctctg ccagggcgcc
      481 ctctttttct ggctacgcat gctggcacgg aacttgcttc aggaaagatc aaggcacccc
      541 ttaaggtgtc gatcgtttag gtgatcagcc gaacgataca ttcttcacta tgaaaggtta
      601 cgatgatgag cgatcacaca aaggaaagat ccatgatgga gcacccatcg cggagaagct
      661 tcctcggatt gagttcggtg gctctggcga cggcggcatt tgcagggatg actgcgaatg
      721 ctcaagagaa gtcgagcacc caaaaagcgg agcacgacca ttcctcgagc gatccgggac
      781 cagagaacaa ggcacttcta ggggaaaacc caaactctaa cactcctcca ccgacggatc
      841 acggcgatgt ggtccccctc tggtattcct tcgatcttgt gaagaagcgc gtcgagcaag
      901 gtggatggac ccacgaagta actcagagcg tcctgccttc atcgagggat atcgctggcg
      961 tcaacatgcg cctcactgcc ggcagctatc gcgagatgca ttggcatacc gctgacgaat
     1021 gggcttacgt actctacggc aatgctcgcg tcaccgtgat gaaccccgat ggcaccatgt
     1081 tcatcggtga cgtgggtgta ggcgatcttt ggctcttccc cgcgggcttt ccacattcca
     1141 ttcagggact tggtccggat ggaactgagt ttcttctggt ctttaaccag gggaacttct
     1201 cggaagatgg aacgatgctt ct
//
LOCUS       DATMKH010000036         1624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_20783,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000036 DATMKH010000000
VERSION     DATMKH010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20783"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(347..1585)
                     /gene="malE"
                     /locus_tag="VNV63_00420"
     CDS             complement(347..1585)
                     /gene="malE"
                     /locus_tag="VNV63_00420"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF007011.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HXC61111.1"
                     /translation="MNRKPLGLWVQAIVSRAAGSSKKSLCFIFVLFSAAQAFAWTDGE
                     LLIWIGSNRAFHALSDIGKTFEEEVGAPVKVETQEKIIDKFQAAAQSGKGPDIFFWAN
                     DRIGEWADSGLLKPLDIKDEFKAKYLPMSWDAVTHKQRVWGYPVALEAVSLIFNKKLV
                     TGKVPTQLSELPAFGKELKAQNPKAIVIMWDYKTPYFSWPFLASAGGYPFKKTAEGYD
                     VQDIGVANTGALEGLKAIVELINTGILPKGSTQSVMGEKMASGQLALMVNGPWDWANL
                     RKAGIDFDFAPIPGVGGNPGRPFVGVFTALINRSSPNLDLAGQFLEEYALTSDGLKAM
                     DADAPLGVPALKTLCDEMATKNHLIKVTYENAENGVVMPNIPQMGKFWSSMRAAFEIA
                     TNGGATPEIALNDALENMER"
BASE COUNT          364 a          435 c          416 g          409 t
ORIGIN      
        1 ggactggatc gtacggcctt tgaggagtga gtcggtttgc ggttgacttc cataaaagtc
       61 attctcctgt accatatagc cctttgcttt ttcttcggac atgtatcggc cctgttagag
      121 ttttgccata gtagcgcacc cctgcagcgt acggcccaag ctgtccggga ggtggtcgat
      181 gaaacttgcc tgcaggccgc acggacccgc gacgatttac ccgactcatc aataagggcc
      241 aacggcaaag actcgatggc gtagaactcg agcagctaat tgtaaacagt tcgagagaag
      301 gctacaattg agcacgcagg agatcatcag agagctgtat tcacggctac ctctccatgt
      361 tctccaaggc atcattgaga gcgatttctg gggtggctcc gccgtttgtg gcgatctcga
      421 aagcagccct catcgaagac cagaatttgc ccatttgcgg aatgttcggc atcacaaccc
      481 cgttttcagc gttctcgtaa gttactttga ttagatggtt cttggtggcc atctcgtcac
      541 agagggtttt aagtgccgga acgccaagag gtgcgtccgc gtccattgcc ttcagaccgt
      601 ccgaagtgag ggcatattct tccaggaact gccccgcaag atcgaggttc gggcttgaac
      661 gattgatcag tgcggtaaat acgccgacaa acgggcgtcc cggattacca ccaactcctg
      721 gaatcggagc aaaatcgaaa tcgatgccgg ctttgcggag attagcccaa tcccacggtc
      781 cattcaccat cagcgccaac tgaccggagg ccattttttc acccattacg ctttgcgttg
      841 atcctttcgg caagatgccc gtattgatga gttccacgat tgccttcaga ccctcgaggg
      901 cgcctgtgtt ggccactccg atgtcttgga catcatagcc ctcggccgtt ttcttgaacg
      961 ggtatcctcc ggcactggcg agaaacggcc agctgaaata gggcgtcttg tagtcccaca
     1021 tgattacgat cgccttggga ttttgggcct taagttcctt accaaatgca ggcagttctg
     1081 acagctgggt aggcactttt cccgtgacga gcttcttatt gaagatgagt gacaccgcct
     1141 caagggcaac cggatatccc caaacccgct gtttgtgtgt cacggcatcc caagacatcg
     1201 gtaggtactt cgccttgaat tcgtctttga tatccagcgg ttttagcaaa cctgaatcgg
     1261 cccattctcc gatccgatca ttcgcccaga agaatatatc cgggcctttg ccgctctggg
     1321 cggcagcctg aaatttgtcg atgattttct cttgggtctc tactttgacc ggagctccga
     1381 cctcttcttc gaaggttttg ccgatgtcgc ttagtgcgtg aaaagctcga ttactaccta
     1441 tccagatcag gagttctcca tcggtccagg caaatgcctg agcagcagag aaaagtacga
     1501 agataaagca gagagacttt ttactggatc ctgccgcgcg ggaaactatc gcctgtaccc
     1561 agagaccgag gggttttcta ttcataaggg gaacgagttt accgacgatc catccattgg
     1621 atca
//
LOCUS       DATMKH010000037         1223 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_20904,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000037 DATMKH010000000
VERSION     DATMKH010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1223
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20904"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1223
                     /locus_tag="VNV63_00425"
     CDS             <1..>1223
                     /locus_tag="VNV63_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015146374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction enzyme endonuclease
                     domain-containing protein"
                     /protein_id="HXC61112.1"
                     /translation="PFGTEDEGVLGDIGIVIVNNMLLTGFDAPVEQVMYLDKIIIAHG
                     LLQAIARVNRVAGVAKDKGFVVDYVGVGNHLKKALDNYDEREQNEVLDVLSFPEEELR
                     ELRASHTAIMEFLEKHGLTDLTDHDAFFDLFYDEDLRFEFMSLFRAFTKCLNVVFPAK
                     EALDFMGNYQSLMEINVLAGKHFRDERLSMKGIPPKLRKITDAFLESRGITVKVEPIS
                     ILDEDFQKDVNSRSRTKTKAAEIEHAIRHHLDVELDDDPDLQASFAEALKKILEEFKD
                     NWNKIFEELENLRCRIIDAGKEPTYGLHRKKQMPLFRMLKKEIFGDTELDEDAISSLV
                     ALTQQLFDEVERELKLTGFWESIPARNKLKADIQRTLLQPEFSKLPGLVQNRARLISR
                     VMEIAEKNNDTILYA"
BASE COUNT          309 a          317 c          340 g          257 t
ORIGIN      
        1 tgccctttgg tacagaggac gagggggtcc tcggtgatat cggtatcgtc attgtgaaca
       61 acatgctgct caccggcttc gatgcgccgg tggagcaggt gatgtatctg gacaaaatca
      121 tcatcgctca cggtctgctc caggccatcg cccgcgtgaa ccgggtcgcg ggcgtcgcga
      181 aagacaaggg ctttgtcgtt gactacgtcg gggtaggcaa ccatttgaag aaggccctcg
      241 ataattacga tgaacgggaa caaaatgaag tgttggacgt gctcagtttc ccggaagagg
      301 aactgcgcga actgagggcc agtcacacgg cgataatgga atttctcgaa aaacacggcc
      361 tgaccgacct gaccgatcat gacgcgtttt ttgatctgtt ttacgacgag gacctccgat
      421 tcgagttcat gagccttttt cgggccttca cgaagtgcct gaacgtggtc tttccagcca
      481 aggaagcgct ggacttcatg gggaactacc agtcactcat ggaaatcaac gttctcgccg
      541 gcaaacactt ccgtgatgaa cgcctgagca tgaagggcat tcccccgaag ttgcgaaaga
      601 tcaccgatgc attccttgaa tcgcggggta tcactgtcaa ggttgagccc atctcgatcc
      661 tcgatgagga cttccagaag gatgttaaca gccggagccg gacgaagacg aaagctgccg
      721 agattgagca cgctatccgg catcatctgg atgtcgagct cgacgatgac cccgatctgc
      781 aggcctcctt tgcggaagcc ttaaagaaga tcctggaaga gttcaaggat aactggaaca
      841 agatttttga agagttggaa aacctccggt gtcgcatcat cgatgcgggc aaggaaccaa
      901 cctacggcct ccaccgtaag aagcaaatgc cgctcttccg catgctcaag aaggaaatat
      961 ttggggacac ggaactggat gaggacgcca tctcttcgct ggtggctctg acacaacaac
     1021 tatttgacga ggttgaacgc gaactgaagt tgacgggctt ctgggagagc attcccgccc
     1081 gcaataagct taaggctgat attcaaagga cgctcctgca gccagagttc tccaagctgc
     1141 cggggctcgt gcaaaatcgt gcgcgcctaa tcagccgcgt catggagatc gctgaaaaga
     1201 acaacgacac aatcctgtac gcc
//
LOCUS       DATMKH010000038         3859 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2908498, whole genome shotgun sequence.
ACCESSION   DATMKH010000038 DATMKH010000000
VERSION     DATMKH010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3859
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2908498"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1347)
                     /gene="rpoN"
                     /locus_tag="VNV63_00430"
     CDS             complement(<1..1347)
                     /gene="rpoN"
                     /locus_tag="VNV63_00430"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HXC61113.1"
                     /translation="MKTRLDLRLTQKLVMTPQLQQAIKLLQLSRLELQQTISQTLLEN
                     PLLEEEIALESSEEETASAEESPAETSETPATTEHSDEGEFSWEDYFQEERREWTSQE
                     PFADSSEERPSYEQTLSKKDSLEEYLLWQLGLSQISEGERAVGELIIGNLDDDGYLRI
                     SLDEVAATAGVPVQQAEKVLNLIQDFDPPGVGARDMKECLLIQVRQLGLQGSLVEAIL
                     KDHLPELETRRYPILAKRFGVSIEEVCHAAKVIEGLEPKPGRPFFTPTQQLMIPDIFV
                     VKTEEGYQVVLSEDGLPRLRVSPYYRRLLSTSNDKKDATRAYIEEHLRSALWVIKSIE
                     QRNRTICRVAESVVKFQQDFFEKGAGHLKPLVLKQVAEDIGMHESTISRATTNKYLSC
                     LHGLFELKYFFTTGISKSGEEGGELSSSTVRELLRKMVAEEDPKQPLTDQEIVASLH"
     gene            complement(1371..2105)
                     /gene="lptB"
                     /locus_tag="VNV63_00435"
     CDS             complement(1371..2105)
                     /gene="lptB"
                     /locus_tag="VNV63_00435"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HXC61114.1"
                     /translation="MDGIVLESKNLKKNLRNRAVVKDASLSIHTGEVVGLLGPNGAGK
                     TTIFYLMVGLLRPDRGDIFIDHECITNLPMYLRARKGIGYLPQEASIFRKMTVEENLL
                     AILEMLDLSARERSDRAQELLREFNVLHLARQKAHTLSGGERRRVEIARALVMSPHFL
                     LLDEPFAGIDPIAVGDIQKIISQLKERGIGVLITDHNVQETLEITDRAYLINEGEIFE
                     AGTPREIASSPKAKAVYLGERFRWQP"
     gene            complement(2133..2660)
                     /locus_tag="VNV63_00440"
     CDS             complement(2133..2660)
                     /locus_tag="VNV63_00440"
                     /inference="COORDINATES: protein motif:HMM:NF015901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HXC61115.1"
                     /translation="MSSPILISKRLFSISLGLITGLWIFQGLAQEPATPPISITSEHM
                     MVRNLENHVVFEGNVHVQKEDFQMDADQMTVNFASGGRGDTLPADALQTNRSVTEKRS
                     VSTIDASGHVKIIKGDRRATSQIAVYDQVAEKVTLTGNPVSWEKDYKVSGTKITIFLK
                     ENRSLVEGSSVLIQP"
     gene            complement(2635..3192)
                     /gene="lptC"
                     /locus_tag="VNV63_00445"
     CDS             complement(2635..3192)
                     /gene="lptC"
                     /locus_tag="VNV63_00445"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HXC61116.1"
                     /translation="MDRIRLALLIIGVIAGIVVVVMISLGSKKKSFSLVPASFVGKPD
                     VEMTDFRFFSTKNGRVEWEIQARSTETFERRHQALLHGVQVTFHNTDGMSMTLQGERG
                     VLDTTSKDFQITNQKDPIQVSMNKEYYLYTSTLDWINKTRQIQSDAPVRIQGPNLEIQ
                     GTGLLANLALQEIQVLHNVVAHFNQ"
     gene            complement(3222..>3859)
                     /locus_tag="VNV63_00450"
     CDS             complement(3222..>3859)
                     /locus_tag="VNV63_00450"
                     /inference="COORDINATES: protein motif:HMM:NF014036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HXC61117.1"
                     /translation="DKLFATLDPTVRKLKLSSKREILVSDTVGFIQRLPHQLIAAFKA
                     TLEEVLEADLLLHIIDMSHPHAENQRASVQKVLEELGADKKPQLEVYNKIDLSAVYAA
                     RPKGAEIQPSGVFISALRGDGIQDLLERMETMLFPSKERWQLVIPAERGDLLARLYQS
                     GKVTGRKNQGSGVRLEAEVPAQLADTFLLFRVMEAGQRPVLAKRVSAARAR"
BASE COUNT          905 a         1030 c          886 g         1038 t
ORIGIN      
        1 atgcaggctg gcaacgatct cttgatcggt tagcggctgt ttagggtctt cctctgcgac
       61 catttttcgg agcagttctc tcaccgttga agaagatagc tcgccccctt cttctccaga
      121 ttttgaaatg cctgtcgtaa aaaaatattt gagttcaaaa agtccgtgca ggcacgatag
      181 atatttattg gtcgtcgcac ggctgatcgt ggattcatgc atgccaatat cttcagcgac
      241 ctgcttaagc accagcggtt taagatggcc cgctcctttt tcaaagaagt cttgctggaa
      301 ttttacaacg ctctcggcca cccggcaaat cgtgcggttt cgctgctcga tgcttttgat
      361 tacccacaag gcggatcgca ggtgttcctc aatatatgca cgcgtcgcat cttttttatc
      421 attgcttgta gacagcaagc ggcgataata aggactcacg cgaagcctgg gaagtccatc
      481 ttcgctcaaa acaacctggt atccttcttc ggtcttgacc acaaagatat ccggaatcat
      541 caactgctgg gtcggcgtga agaacggtct gcccggttta ggttcgagcc cctcgatcac
      601 ctttgccgca tgacagacct cttcgatgct gaccccgaac cgcttcgcca gaatgggata
      661 acgccttgtc tcaagttctg ggaggtgatc tttcagaatg gcctccacca aggatccttg
      721 caagcccagc tgacgaacct ggatcaacag acattctttc atgtccctcg ccccaacacc
      781 cggaggatca aaatcctgga tgaggtttaa gaccttttca gcctgttgaa ccggtacccc
      841 ggcagtcgca gccacttcat cgagagatat acgaagatac ccatcatcat ccaggttgcc
      901 gataatcagt tcccccacgg cccgctcgcc ttcgctgatc tgtgataatc cgagctgcca
      961 aagcagatat tcctccagag aatccttttt tgagagtgtc tgttcatagg acggccgttc
     1021 ttcagaagaa tccgcaaagg gttcttggct cgtccattcc cgtcgttctt cctgaaaata
     1081 atcctcccaa ctaaattctc cctcgtcaga atgttctgtg gttgcgggtg tctcggatgt
     1141 ctctgcgggg ctttcctctg cgctggccgt ttcttcttca ctagattcca atgcgatctc
     1201 ttcctctaaa agagggttct ctaacaaggt ctgcgagatc gtttgttgca gttcaagcct
     1261 ggaaagctgc agcaatttaa tcgcttgctg aagctgaggc gtcatgacta acttttgcgt
     1321 taatcttaaa tccagtcttg ttttcataat cgtcgcataa tacgaattat ttacggctgc
     1381 caccggaacc gttctccgag ataaaccgct ttggcctttg ggctgcttgc gatctcgcga
     1441 ggtgttcctg cttcgaagat ctctccttca ttgatcaggt aggcgcgatc cgtaatctct
     1501 aaggtctcct gtacattgtg atccgtaatg agaacgccga tacctcgttc cttaagctga
     1561 gaaatgattt tttggatgtc tcccaccgca atcggatcaa tgcctgcaaa aggctcgtcc
     1621 aaaagcagaa aatgtggcga catcacgagg gcacgtgcaa tctccacacg acggcgttca
     1681 ccccctgaga gggtatgggc tttttgccgg gcaagatgca agacattgaa ctctctgagc
     1741 agttcttgag cgcgatcgct tcgttcacgc gcagaaagat ccaacatctc caggatggca
     1801 agaagatttt cttccacagt catctttcga aagatcgaag cctcttgggg aagataccct
     1861 attcctttcc tcgcacgaag atacatcggt agatttgtga tgcattcatg atcgataaaa
     1921 atatcgcctc gatcaggccg caggagtcct accatcaggt aaaagattgt cgtcttgcct
     1981 gccccgttgg gccccagaag tcctaccacc tcaccggtat gaatgctcaa actcgcatcc
     2041 ttcacgacag cacgatttcg aaggttcttt tttagattct tcgattctag gacgattcca
     2101 tccatttaat taagatccat tcatcaatat tgtcacggtt gaatgagaac actgctgcct
     2161 tcaaccaggc tcctattttc ttttagaaag atggttatct tggttccgct gactttgtag
     2221 tccttttccc atgacaccgg atttccggtt aaagtcacct tttccgctac ctgatcataa
     2281 acagcaatct gggaggttgc acggcgatct ccctttataa ttttaacatg gccgctggca
     2341 tcaatggtag agaccgatcg tttctccgtc acggatctgt tggtctgcaa cgcatccgcc
     2401 gggagagtat cccctcgacc gccagacgca aagtttacag tcatctggtc tgcatccatc
     2461 tgaaaatcct ctttttgcac atggacgttt ccctcaaaca ccacatggtt ttccaggttc
     2521 cggaccatca tgtgttctga agtgatcgag atcggagggg tcgcaggctc ctgggccaag
     2581 ccttgaaaaa tccaaagacc cgtgatgagg ccaagagaga tcgagaataa tcttttactg
     2641 attaaaatgg gcgacgacat tgtggagaac ctgaatttct tgtaaggcca gattcgccag
     2701 aagacctgtg ccttggatct ctaggttggg cccctgaatt cttacaggag catcggattg
     2761 gatctggcgg gtcttgttta tccaatccaa ggtagaagtg taaagataat attctttgtt
     2821 cattgaaact tgtataggat ctttctggtt cgtgatctga aagtcttttg aggtcgtgtc
     2881 cagaacccct ctttcgcctt ggagcgtcat actcattcca tccgtattgt ggaatgtcac
     2941 ttgaacacca tgaagcaagg cctggtgtct ccgttcaaag gtctccgtag atcttgcttg
     3001 gatctcccac tcgacccgcc cgtttttggt ggagaaaaac ctaaaatccg tcatctcgac
     3061 atccggcttc cctacaaagc tggcagggac cagagagaaa ctcttctttt ttgaaccgag
     3121 actgatcata acaacgacca cgatgcccgc gattacgcca atgatcaaca aggctaaacg
     3181 tattcgatcc attgacacaa ccaacgtcct cttttcaaaa atcagcgagc ccgagccgcc
     3241 gacaccctct tggcaagtac tgggcgctga ccagcttcca tgacacggaa aagcagaaag
     3301 gtgtcagcca gttgcgccgg cacttctgct tccagtcgaa cgccagaccc ttgattcttg
     3361 cgacccgtga ccttaccgga ctgataaagc ctggcaagaa ggtctccgcg ttcagcaggt
     3421 attaccagct gccatcgttc ttttgatgga aagagcatcg tctccatccg ttcaaggagg
     3481 tcctgtatac catcaccgcg cagggccgat atgaaaacac cgctgggctg aatctcagcg
     3541 cctttgggcc gggctgcata tacagccgat aaatctattt tgttataaac ctcgagctgc
     3601 ggttttttat cggcgcccag ctcctctaat accttttgca ccgatgcccg ctggttctcc
     3661 gcatgggggt ggctcatgtc aatgatgtgc aagaggagat ccgcctccaa cacttcctcc
     3721 aacgttgcct tgaaagcagc aatgagctga tggggaagac gctgtatgaa gccaacggtg
     3781 tccgacacaa ggatttcgcg cttgctggaa agcttgagtt tcctcaccgt ggggtccaag
     3841 gttgcaaaca gtttgtcct
//
LOCUS       DATMKH010000039         1233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2267474, whole genome shotgun sequence.
ACCESSION   DATMKH010000039 DATMKH010000000
VERSION     DATMKH010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2267474"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1233)
                     /locus_tag="VNV63_00455"
     CDS             complement(<1..>1233)
                     /locus_tag="VNV63_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015030598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXC61118.1"
                     /translation="IVLMRKGENPDRVIKGIKAEVAKLNNSVLPQDTKLVTFYNREDL
                     INYATHTVLHNLIEGILLVTLIVSLFMFNWRTTLIVSIIIPLSLLFAFICLHLMGMSA
                     NLLSLGAIDFGIIIDGAVVMVEGLFVQLDKRAHEMGMEKFNKLSKLGIIRQRGAELGK
                     AIFFTKVIIITALLPIFSFQKVEGKMFSPLAYTLGFALLGALIFTLTLVPVMAGILLK
                     KNIKDKRNPIVHFMTASIMRGFRFVFHHKRLSIAVAIIGVTAGLYAFRFLGSEFLPEL
                     NEGSIWVRATLPYSISLDTSVAKARQMQEIMMRFPQVRRVMCQTGRPDDGTDVAGFYN
                     NEFGVALYPEEEWKPRISKEELIDTMNKALSIIPGIDYNFSQPIMDNVEEAVSGVKGS
                     ICVKIYGDSLDYMEDESQR"
BASE COUNT          296 a          292 c          362 g          283 t
ORIGIN      
        1 acgttgggat tcatcctcca tataatcaag cgagtcgccg tatattttca cgcagatgga
       61 acctttcacc cccgagacgg cttcttcgac gttatccatg atcggctggg agaagttgta
      121 gtcaatccct gggatgatcg acaacgcttt attcatggtg tcgatcaact cttccttgct
      181 gatcctgggt ttccattctt cttcgggata gagcgcgacc ccgaattcgt tgttgtaaaa
      241 gccggcgacg tcggtgccgt cgtcagggcg accggtctgg cacatgacgc ggcggacctg
      301 tggaaagcgc atcatgatct cctgcatttg gcgggccttt gccacggaag tatccagcga
      361 gatgctgtag gggagagtag cacgaaccca gatcgagcct tcgttgagct cgggcaggaa
      421 ctccgaaccc aggaagcgga acgcatagag accggctgtt accccgatga tggcaacggc
      481 tatggacagt cgcttatggt ggaagacaaa gcggaaaccc cgcataatgg acgcagtcat
      541 gaaatggacg atggggttgc gcttatcctt gatgttcttt ttcagcagga tgccagccat
      601 gacgggcacg agtgtgaggg tgaagatcag ggcgccgagg agggcgaatc ccagtgtata
      661 ggcaagcgga gagaacattt taccttctac cttttggaag gagaagatag gcagcagagc
      721 ggtgatgata atgacctttg taaaaaagat ggcctttcca agctctgccc cgcgctggcg
      781 gatgatgccc agtttggaca gcttgttgaa cttctccatt cccatttcat gggcgcgttt
      841 gtccagttgg acgaaaaggc cttcgaccat tactacagcg ccatcgatga tgatcccgaa
      901 gtcgatcgcc cccagtgaga ggaggttggc agacataccc atgaggtgta ggcagataaa
      961 ggcaaaaagg agcgacaagg gaataatgat agaaacgatg agggtggtcc gccaattgaa
     1021 catgaaaagc gaaacgataa gggtgaccag caaaatgcct tcgatgaggt tgtgaaggac
     1081 ggtgtgggtg gcgtagttga tcaaatcttc ccggttatag aaagtgacga gcttggtgtc
     1141 ctggggcagg acgctgttgt ttagtttcgc cacttcggcc tttatccctt tgatgacccg
     1201 gtccggattc tcgcccttgc gcatcagcac gat
//
LOCUS       DATMKH010000040         1900 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3870163, whole genome shotgun sequence.
ACCESSION   DATMKH010000040 DATMKH010000000
VERSION     DATMKH010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3870163"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            325..1593
                     /locus_tag="VNV63_00460"
     CDS             325..1593
                     /locus_tag="VNV63_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61119.1"
                     /translation="MGPTDDTLKQRMVDYIVENTDDLSFTKHFWRECEPLPRDVENHS
                     KFASIVSEFEKGIDPKPMQMGVGLGTSYAWATMEAMPKLGHYLRALVELGEPTKGRVW
                     LTMECTHGYANPIGQFIQVPEKIETWKDVEEVIHQVTQIGEASTQFGKAYLFGFFVGM
                     IIGDAHKPKQGRAHRNVHIVLSKKYDTNVKIGDFTTLSANQLGLRMNRLVDVPKPDDK
                     PFGFYQWVSQSSPFVDWIFNVILGLKDGQHTTYDEVRMEWALDAPLEFRRGLLHGIAE
                     SDGSVAPASQVVEFWVIPDWEFMIKLLATFGFKGFRNREAVSLVKSQAISSFRIPVFA
                     PHLRTVRYSLHELMATTRKLSKEERLPLDVRSEIIGLASQGWSVPRIVVEIAKTKKLL
                     VSFEAAQRWAKKSGKYQPKSRPDSREERDQ"
     gene            complement(1619..1786)
                     /locus_tag="VNV63_00465"
     CDS             complement(1619..1786)
                     /locus_tag="VNV63_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011998757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S28e"
                     /protein_id="HXC61120.1"
                     /translation="MVGRTGVAGEITQVRVKILEGKEKGRILTRNVKGPIRLADILLL
                     RDTEREARKIR"
BASE COUNT          458 a          492 c          524 g          426 t
ORIGIN      
        1 tcactcaccg gatcccagcg cgctctggaa taccatgaag ataaggacga agacgtagat
       61 tcccagattc acgtccccgc tgagttcgga gctcgtcgcg gcgcccgcta tcacggggac
      121 gctcaggctg aggacgaaga cggtcatcac ggtcgcaaaa atcccgtctg aaagggcctc
      181 gagtctgcct ttgctgaggc cgagggtgga ttggtcgctg gtcaaactta gagagcaagg
      241 gaggttgcga tttagcatca aataaaggaa gaatcgcgtt tccgaacgca tggtcgccta
      301 aatagcgccc gcgacatcgt aaacatgggg cctaccgacg acacactaaa gcaacgaatg
      361 gtcgactaca tcgtcgagaa cacagacgac ctgagtttca cgaagcattt ttggagggag
      421 tgtgagcctc tgcccaggga tgtcgagaat cactccaagt tcgcctcaat tgtctcggaa
      481 ttcgagaagg ggattgaccc gaaaccgatg caaatgggcg ttggactggg cacatcgtac
      541 gcgtgggcga ctatggaggc gatgcccaag ctaggacact atctgcgagc tttggttgag
      601 ctaggcgaac cgacaaaagg cagggtctgg cttacgatgg aatgtaccca tggatacgcc
      661 aatccgattg gccagttcat ccaagtaccc gaaaagatcg aaacatggaa ggatgtggag
      721 gaggtgattc atcaagttac acaaatcgga gaagcgtcta ctcaatttgg caaggcatac
      781 ctctttggat tttttgttgg aatgatcatc ggcgacgcgc acaagccaaa gcagggtcga
      841 gctcatagga atgtccacat cgtccttagc aagaagtacg acacaaacgt gaagatcggt
      901 gacttcacca ctcttagcgc caatcaactt ggactccgaa tgaatcggtt agtcgatgtc
      961 ccgaaacccg atgacaagcc attcggattt tatcagtggg tgtctcaatc gtccccattc
     1021 gttgactgga ttttcaatgt gattctcggc ctgaaagatg gacaacacac tacgtatgat
     1081 gaggtccgga tggaatgggc tctggacgcg cctctcgaat tccgacgggg cctgcttcac
     1141 ggcatcgccg agtctgatgg ttcggtcgcc ccggcaagcc aggtggtcga gttctgggtg
     1201 atacctgatt gggaattcat gatcaagcta ttggcaacat tcgggttcaa agggtttcgg
     1261 aatcgggaag ctgtttccct cgtcaagtct caggcgatca gttcgttccg gattcctgtt
     1321 ttcgcgccac atcttcggac cgtgagatac agtttgcacg aactcatggc aactaccagg
     1381 aaactctcga aggaagaaag gttgcccttg gatgtcaggt cagaaataat cgggcttgca
     1441 agccaaggat ggtctgttcc gagaatcgtc gtcgaaatag cgaaaactaa gaaactgctc
     1501 gtctcgttcg aggccgctca acgttgggcg aagaagagcg ggaaatatca accgaagtct
     1561 cgcccagatt ctcgagaaga aagggaccag taaagagcga cagaaagtaa gatgcgtctt
     1621 acctgatttt ccgagcttct ctttcggtat ctcgcaatag caggatatcc gcgagcctga
     1681 ttgggccctt tacgttcctg gtgaggatcc ttcccttctc ctttccctcc aggatcttga
     1741 ccctgacctg ggtgatctca ccggccaccc cggtgcgccc gacgatctgg acgacctcgg
     1801 caggggtgat cgattctgcg ctctgcctgc tgccgctgct gctcaattat cgttacgcct
     1861 ttgctgtggc ggtctgggcc gccccgcccc tcacccttgc
//
LOCUS       DATMKH010000041         1303 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_23709,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000041 DATMKH010000000
VERSION     DATMKH010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1303
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23709"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="VNV63_00470"
     CDS             complement(<1..338)
                     /locus_tag="VNV63_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61121.1"
                     /translation="MKNNSKAGPVSTDTESNRLEETVVRFCRCARANGLGSGRTTIDC
                     LQAIRIVQDAGVAAIEDALRSVLCSSKDEWDLFAKLFALIWEGPAPTGPSPKKSGKNR
                     FSTHGLGQEK"
     gene            complement(335..1243)
                     /locus_tag="VNV63_00475"
     CDS             complement(335..1243)
                     /locus_tag="VNV63_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HXC61122.1"
                     /translation="MALPNSNSGTGQIPSIDALIDRSRALGYVMDRSLGTAIYLLVRL
                     GKPLLIEGHAGVGKTEAAKVLASILGTELIRLQCYEGLDVNSAVYEWNYQKQLLAIKI
                     QEGSDRKIQDLEEHIFGPDFLLRRPLLKSISCEETSPVLLVDEIDRADEAFEAFLLEV
                     LGEFQISIPELGTIKAKKAPYVVLTSNGARDLSDALKRRCLYHWIDYPSFEKEMDIVQ
                     AKLPGIGAELGTQVVRFLHSLRKMSLSKIPGVAETLDLSTALALLEKTDLDQETANQM
                     IGCVIKSADDLVKVREEGVDKLLASR"
BASE COUNT          277 a          370 c          324 g          332 t
ORIGIN      
        1 tttttttcct ggcctaatcc gtgagtggaa aaccggttct tccccgactt cttaggggac
       61 ggtcctgtag gcgccggacc ctcccagatt aaggcgaaca gtttagcgaa caggtcccat
      121 tcatctttcg aagaacagag cacggatctg agtgcgtcct cgattgccgc tacgccagca
      181 tcttgcacga tccttatcgc ttgtaagcaa tcgatagtcg tcctaccgga gcccagcccg
      241 tttgcacgag cgcaacggca gaatcgtacc accgtttcct caagacggtt cgactcagtg
      301 tccgtcgaaa cgggtccggc ctttgagtta ttcttcatcg ggacgcgagc agtttatcga
      361 cgccctcttc ccggactttg acgagatcat cggcagattt gatgacacaa ccgatcattt
      421 ggttggcggt ttcttggtcc agatctgtct tctccagaag cgcgagcgcg gtcgatagat
      481 ccaaggtttc ggccacgccc ggaatcttgg aaaggctcat ctttctaagc gagtgcaaga
      541 acctaaccac ttgcgtaccg agttccgccc cgatccctgg gagcttcgct tgaacgatgt
      601 ccatctcctt ttcgaagctg ggataatcaa tccaatgata aaggcatctt ctctttagcg
      661 cgtcactcag atctctggcc ccgtttgaag tgagaacaac gtagggcgcc ttctttgcct
      721 tgatcgttcc tagctccggg atactgatct ggaattcacc tagtacctcg agcaggaatg
      781 cttcgaatgc ttcatccgct cgatcaatct cgtccactag gagtaccggg gaggtttctt
      841 cgcaggagat cgatttcagg agtggccgcc ttagtaagaa gtccggacca aagatatgct
      901 cctcgagatc ttggatcttt cggtcgctcc cttcctgaat tttgatcgcg agcagctgtt
      961 tctgatagtt ccattcatat accgccgaat taacgtctag cccctcataa cactggagcc
     1021 ggatcagctc tgttcccagg atggacgcaa gcaccttagc tgcctcggtc tttcccaccc
     1081 ctgcgtgccc ctcgatcagt aagggtttac ctagccgaac tagcagatag atcgcggttc
     1141 ccaacgatcg atccatgaca taacccagag cgcgggaccg gtcgattaga gcgtcgatgc
     1201 tcggaatctg tccggttcca gaatttgaat ttggcaaagc catcgttggc gttatcgacc
     1261 ggtgcggcga cggacctggc cctaaactag ctatttttcg gag
//
LOCUS       DATMKH010000042         1008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4514599, whole genome shotgun sequence.
ACCESSION   DATMKH010000042 DATMKH010000000
VERSION     DATMKH010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4514599"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="VNV63_00480"
     CDS             <1..364
                     /locus_tag="VNV63_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXC61123.1"
                     /translation="SDSAFTSLKDQVDRVVTTFYHLPGIPFYSLSHWIYDLYFWGCLE
                     NVSPINVIAKLSPRPILLIAGQTDDRMTAMDAYRLFEAASQPKEQWIVPGAGHGETLA
                     MAGQTYPERVVDFFKRAL"
     gene            373..795
                     /locus_tag="VNV63_00485"
     CDS             373..795
                     /locus_tag="VNV63_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HXC61124.1"
                     /translation="MSKSSVAIIGASDQRDKFGNKAVRAYLRQGFTVYPVNPKESRIE
                     GLPSFRSILDIPGPVDHASFYVPPDVGLKVIAEMAKKGVREVCLNPGSESPELIEKAR
                     RLGIEPIVTCGILASPGGFSDPSPVSLVFDRGTEGSYI"
BASE COUNT          217 a          258 c          277 g          256 t
ORIGIN      
        1 atcggacagc gctttcacca gcctgaagga tcaggtggac cgtgttgtga ccaccttcta
       61 tcaccttccc ggaattccgt tttattcttt gtcacactgg atctatgatc tctatttttg
      121 gggttgcctg gaaaatgttt caccgatcaa cgtcatcgcc aaactttctc caagacccat
      181 tttgcttatt gcgggtcaaa ccgacgatcg gatgacggcg atggatgctt atagactctt
      241 tgaagcagct tctcagccta aggagcagtg gatcgtgccg ggcgccggtc acggcgagac
      301 attggccatg gcggggcaga cttatccgga gcgcgtggtg gacttcttca aacgcgccct
      361 ttaagtggtt tgatgtctaa atcgtcggtt gcaattattg gcgcctcgga ccagcgtgac
      421 aagtttggga ataaggcggt tcgcgcctac ctccggcagg gattcacggt ttatccggtg
      481 aatcccaaag agagccggat cgaaggcctc ccctcatttc gttcaatcct ggacattccg
      541 ggcccggtgg atcatgcaag cttctatgtt ccgcccgacg tcgggctgaa ggtcatcgca
      601 gagatggcga aaaagggtgt ccgggaggtt tgtttgaatc ctggttccga gagtccggag
      661 ctcatcgaga aagcaaggcg tctgggtatc gagccgattg tgacttgcgg catcttggcg
      721 agtccaggag gtttctcgga cccttccccc gtgtctttag tttttgacag agggacggaa
      781 gggagttata tttagaacag tttgtctaac cggttcgata gcggaataac gaacgaaacg
      841 tagggccgtg aaacaaacaa tacaaaaaca agtcgccaag ctgttgatct tcagcgggat
      901 cctcttccca acgattctct ttgggtgtgc agcagctagg ttgcccttcg ttccgtttcc
      961 taccggcatt accccgccca gaacagtcgc ggtcatgccg ctcgataa
//
LOCUS       DATMKH010000043         2525 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_345488,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000043 DATMKH010000000
VERSION     DATMKH010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345488"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1304)
                     /locus_tag="VNV63_00490"
     CDS             complement(<1..1304)
                     /locus_tag="VNV63_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXC61125.1"
                     /translation="MEKILVVDDEKSMRDFLGLVLKKEGYFVAVASEGEEAIKVLQKD
                     IFDLVITDVKMPKLSGIDVLKAVKETASETLVIMITAFASAETAIEALKEGAYDYITK
                     PFQIDEVKLIIRNALEKRRLRAENLLLRRELKGLATFENIIGKSEKIQKVFETVRKVA
                     DTTSNVLILGESGTGKELIARAIHFDSRRKDKPFVTVNCSALPETLLESELFGHMKGS
                     FTGAISNKEGLFEVANGGTIFLDEIGETSPAIQVKLLRVLQEKEFRRIGGTKDVRVDV
                     RIIAATNKDLDRAVAEGTFREDLYYRLDVIPIHLPPLRERLEDIPLLAQHFLSKFSQT
                     AGKNIKGITPEAIQLLKSQEWRGNVRELENVIERVIAFITGDMIDSEDLRGCFQKPSQ
                     KDAWALPLPEEGLNLEELLNRLEGEFLLKALERTRWVKKEAA"
     gene            complement(1289..>2525)
                     /locus_tag="VNV63_00495"
     CDS             complement(1289..>2525)
                     /locus_tag="VNV63_00495"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXC61126.1"
                     /translation="NLTAFYTVAYLSGRLAEQLRQTGESLKEKAKGLIQLQAFHENVV
                     QSMSGGLFTTDLEGRVTSFNRAGEEITGFTKEEILGKSWWELFSQKDPKVFGYRLETA
                     DAPFRFDTETQRKEGASLLLGVTLAPLLDDRGQKTGMVGMFQDLTKIRAIEEEMKKRE
                     RLASIGELAAGMAHEIRNPLAAMSGSMQVLSRELKLEDEHRRLMEIALKETERLNMII
                     TTFLRYARPTPLNKKRSSLYDLLQDTLSLLRNSPDYREHISLVTKFTRGKIWMMMDPD
                     QMKQVFWNLVLNAMEAMPLGGQLTIETRKPKKTMPRFADHAPAPSIIEVRFIDTGAGI
                     NPADLERIFYPFFTTKQKGSGLGLSIVHRIIEEHGGQILVESKLGQGTTIVLQLPVET
                     IEQNLPEHDSEGAARWKRS"
BASE COUNT          561 a          724 c          575 g          665 t
ORIGIN      
        1 ttcgccgcct ctttcttgac ccaccgcgtc cgctccaaag ccttcaggag gaactctcct
       61 tccagccgat tgaggagctc ttccaggttg agtccctcct ccggcaatgg aagcgcccag
      121 gcatccttct ggctaggctt ctggaaacat cctcgaagat cttcggaatc gatcatatcg
      181 cctgtaatga aagcgatgac acgctctatc acattttcga gttctctcac gttccctcgc
      241 cattcctggg acttcaaaag ctggatggcc tcaggagtga tccccttgat gttcttgccg
      301 gctgtttgag aaaacttgct caagaaatgt tgagccagaa gcggaatatc ttccaatcgc
      361 tccctcaacg gcggaagatg gatcggaatg acatcgagac ggtaatagag gtcttcacga
      421 aaggtccctt ccgccacggc ccgatctaga tctttgttgg tagccgcgat aattcgaaca
      481 tccacccgga cgtctttagt ccccccgatt cgtcgaaact ctttctcctg aagcacccgg
      541 agcaatttca cctggatagc tggggaggtt tctccaatct catccagaaa aatcgtcccg
      601 ccgtttgcaa cctcaaacag accttcctta ttgctgatcg cccctgtaaa cgagcccttc
      661 atatgcccaa agagctcgct ctccaaaagg gtctcgggta gagcgctgca gttcaccgtt
      721 acaaagggct tgtcttttct gcggctatca aaatgaattg cacgggcgat gagctctttt
      781 ccggttccgc tttcccccaa gatcaaaaca ttgctggtgg tatccgccac ttttcttacg
      841 gtctcaaata ccttctgtat cttttcgctt tttccgatta tattctcaaa agtggcgagt
      901 ccttttagtt cccgacgcaa gaggaggttc tcggcacgaa gacgcctttt ctcaagggca
      961 ttccggatga tgagcttgac ctcatcgatc tgaaacggtt tggtgatgta atcatacgcc
     1021 ccttctttga gggcttcgat ggcggtttcc gcggatgcaa aggcggtgat catgatcaca
     1081 agtgtctcgc tggcggtctc tttcaccgcc tttaagacgt cgatcccgct cagtttcggc
     1141 attttgacat ctgtaatcac caggtcaaag atatccttct gcaggacctt aatggcttct
     1201 tccccctccg aggcaaccgc cacaaaatat ccctccttct taagcaccag gcccaagaag
     1261 tcccgcatgc ttttttcatc gtcaacgact aagatctttt ccaacgggcc gctccttccg
     1321 aatcgtgctc aggcaagttc tgttcaatcg tctcaacagg cagttgaagg acgatggtcg
     1381 tcccctggcc tagcttgctt tccacaagga tctgccctcc atgctcttca atgattcgat
     1441 gaacaatcga aagacccagg ccggagcctt tttgtttcgt ggtgaaaaag ggataaaaga
     1501 ttctctctag atccgccggg ttgatcccag caccggtatc tataaatcgc acctcaatga
     1561 tggagggagc cggcgcatga tctgcaaaac ggggcatcgt ttttttaggc tttcgggtct
     1621 cgatggtgag ctggccgccc agcggcattg cctccatggc attgagaacc aggttccaga
     1681 aaacctgctt catctgatcc ggatccatca tcatccagat cttgcccctt gtaaatttcg
     1741 tgaccaaaga gatatgttcc cgatagtcag gactattgcg cagaaggctc aacgtgtcct
     1801 gaagcagatc atagaggctg ctgcgcttct tatttaacgg cgtcggccgg gcatacctga
     1861 gaaaagtcgt aataatcata ttgagccgtt cagtctcttt caaggcgatc tccatcaggc
     1921 ggcgatgctc atcttctaac ttcaattcac gactcagcac ctgcatcgaa ccgctcatgg
     1981 ctgccaacgg attccgtatc tcatgggcca tgccggctgc caactctccg atgctggcca
     2041 atctctctct tttcttcatc tcctcttcga tggcccgaat cttggtgagg tcttgaaaca
     2101 ttccgaccat gccggtcttc tgaccccgat cgtctaagag gggggccagg gttacgccaa
     2161 gaagcaaaga agcgccttcc tttcgttggg tttcggtatc aaagcgaaac ggagcatctg
     2221 cggtctctaa acggtagccg aaaactttgg ggtctttctg tgaaaataac tcccaccacg
     2281 acttgcccag gatttcctct tttgtaaaac cggtgatctc ctctccggca cggttaaaag
     2341 aggtaacccg tccttccaga tcggtcgtaa aaagaccccc gctcatactc tgcaccacat
     2401 tctcatgaaa agcctgaagc tgaattaaac ccttggcctt ctctttgagg ctctcccccg
     2461 tctgtcgtag ctgctccgcg agtctcccgc tcaagtaggc gaccgtatag aatgcggtta
     2521 gattg
//
LOCUS       DATMKH010000044         2187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3552024, whole genome shotgun sequence.
ACCESSION   DATMKH010000044 DATMKH010000000
VERSION     DATMKH010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3552024"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="VNV63_00500"
     CDS             complement(<1..348)
                     /locus_tag="VNV63_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61127.1"
                     /translation="MKRMLTFSLTVHLTAGILFVFLAWIGERRIESSVYTVNLITPVS
                     ETKETAPEPKKEQAPKIAKPPAAKPAPPKAAKPVSPAKETKRPPASLSRFEQQIAKLQ
                     KQVSSPEPPKSAVQ"
     gene            complement(382..768)
                     /locus_tag="VNV63_00505"
     CDS             complement(382..768)
                     /locus_tag="VNV63_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HXC61128.1"
                     /translation="MAEINVVPLVDVVLVLLNIFMVTAPLLYRGIDINLPRSSSNTIQ
                     PEERVVITVEKNQQIFIDKDRVTVDRVEPLLETIKLRNPAVTVYLRADREVPYGLIVQ
                     VMDAIKRVGIDKLGMVTEPLAEQRTP"
     gene            complement(798..1520)
                     /locus_tag="VNV63_00510"
     CDS             complement(798..1520)
                     /locus_tag="VNV63_00510"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HXC61129.1"
                     /translation="MPTSAFQIDIMDLIASSSLVTKLVILILLVLSVVSWAIIFYKAK
                     YFRKAKRENRQFLEVYDQSDEPFRLKKTAERFPASPLANVFLQAIRFQDPGEAKIETD
                     SPAFNGGDEPGRAYRSERLLRSAGQEILSQMEEYLSFLASTGNVSPFIGLFGTVLGII
                     NAFQEIGRQGTASIAAVAPGVAEALLATAAGLFVAIPSVVAYNYFVNKLRIMTSELEI
                     FTTECMILLEQKQRNSTVVRSR"
     gene            complement(1549..1625)
                     /locus_tag="VNV63_00515"
     tRNA            complement(1549..1625)
                     /locus_tag="VNV63_00515"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1589..1591),aa:Asp,seq:gtc)
     gene            complement(1645..1720)
                     /locus_tag="VNV63_00520"
     tRNA            complement(1645..1720)
                     /locus_tag="VNV63_00520"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1685..1687),aa:Val,seq:tac)
     gene            1838..>2187
                     /locus_tag="VNV63_00525"
     CDS             1838..>2187
                     /locus_tag="VNV63_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61130.1"
                     /translation="MNEVSKHRRDYLFCVVFLVFISAVIASACRRAENTPLPADPGPQ
                     AGTASPESPSNPVAAGPITSSLQSRITNFETDVNIKTMALEGDYLWMGLSTGIIRYDT
                     RTKDQHQVYTTRSTG"
BASE COUNT          530 a          622 c          534 g          501 t
ORIGIN      
        1 ttgtacggcg gacttcggcg gttccggcga agaaacctgc ttctggagtt tagcaatctg
       61 ttgctcgaac cgggacaggc tggccggcgg acgttttgtc tccttcgcag gagaaaccgg
      121 tttagccgcc ttgggaggag ccggcttggc cgcaggcggt tttgcgatct taggagcctg
      181 ctcttttttc ggttccggag ccgtttcttt tgtctcagat actggtgtga ttagattcac
      241 ggtgtacacc gaactttcaa ttcgcctctc accgatccat gcaagaaaca caaacagtat
      301 tcccgccgtc aagtggaccg tcaaagaaaa tgtcagcatc cgcttcatct cagggatcca
      361 tgctttttcc gccatccttt cctaaggggt acgctgctct gccaatggtt cggtcaccat
      421 gccgagctta tcaatcccta cccgcttgat cgcgtccatc acctgaacaa tcaaaccgta
      481 cggaacctcc cgatcagccc ggaggtaaac cgtgacggcc ggattacgca gcttgatcgt
      541 ttccagcaaa ggctcgaccc ggtctaccgt cacccgatcc ttgtctataa agatctgctg
      601 gttcttctcg accgtaatca ccactctctc ctcgggctga atcgtgttgc tggaggatcg
      661 agggagattg atgtcaattc ctcggtacag gagaggcgcg gtcaccatga aaatgttcag
      721 caggaccaag accacatcca ccaacggaac cacattgatc tcggccataa aggaacgctt
      781 ctgaccggtc tggaacatca tcgggaacgg actactgtac tgttgcgttg tttctgttcc
      841 agtaaaatca tgcattctgt ggtaaagatc tcaagctccg aggtcatgat gcgcagtttg
      901 ttcacaaagt aattatacgc gaccacggat gggatggcga caaaaaggcc cgccgcggtg
      961 gccaacaaag cctcggcaac accaggcgcc acggccgcga tgcttgcggt gccctgccgt
     1021 ccgatctcct ggaaggcgtt gatgatcccc aggacggtcc cgaagagccc aatgaagggc
     1081 gatacatttc cggtgctggc taggaaagag aggtactctt ccatctgcga aagaatctcc
     1141 tggcccgcgc ttcttaagag ccgttccgac cggtaagccc ttcccggttc atccccgcca
     1201 ttgaacgccg gggaatccgt ctcaattttg gcctcgccgg ggtcctgaaa gcggattgcc
     1261 tgaagaaaaa cattcgcgag aggagaggcc gggaatcgct ccgccgtctt ttttagtctg
     1321 aaaggttcat cggactgatc atacacctca agaaactggc gattctctct cttggccttt
     1381 cgaaaatatt tggctttata gaaaataatc gcccaggaca ccacagagag aaccaaaaga
     1441 atcagtatca ccagcttggt caccaaactg gaggaagcga tcaggtccat aatatctatt
     1501 tgaaatgcag aagtgggcac actcttctcc taatttatac tcattaattg gcggggccga
     1561 cggggatcga acccgcgacc tccggcgtga caggccggcg ttctaaccag ctgaactacg
     1621 gccccgcatt aaaacagctt gctatggtag gcggaacagg tgtcgaacct gtgacctctg
     1681 ccttgtaagg gcagcgctct accggctgag ctatccgccc gtcttccgac taaaattccc
     1741 tattgcgccc caaaaaaata tgacgtaaaa tactaacaac tttgccaacc attgtcaatg
     1801 acaaatctaa taaatccgat gcaatgaaat aaaaatcatg aatgaagtat caaaacatcg
     1861 tcgagattat ctgttctgcg ttgtctttct cgttttcata agcgcagtca tcgcgtctgc
     1921 ctgccgccgt gctgaaaaca ccccgcttcc ggcagatccc ggcccgcagg ccggaacagc
     1981 gtcacccgaa tcgccgtcca atcccgttgc agccggtccg atcacgagtt ctcttcagtc
     2041 gcgcatcacc aacttcgaga ccgatgtgaa cattaaaaca atggctcttg aaggcgacta
     2101 tctctggatg ggactctcta ccggaatcat ccggtacgac acgcgcacaa aagaccagca
     2161 ccaggtttac acgacacgat ccaccgg
//
LOCUS       DATMKH010000045         1287 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_987478,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000045 DATMKH010000000
VERSION     DATMKH010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_987478"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1287
                     /locus_tag="VNV63_00530"
     CDS             <1..>1287
                     /locus_tag="VNV63_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HXC61131.1"
                     /translation="LDLAVQAAKAAFPLWCSKTWNEPRDLLRNAMEELKAHTSELCTI
                     LTAENGRPYSLAQFETTWIFETYVPALLQMKLPDSTWNQTGVGQITKRYVPLGVVAAI
                     SPWNLPFLLSFTKVLPSLLAGNTVVLKPAVFTPLTVLRAADYIREILPPGVLNVISGG
                     DDLGPWMTSHPDFQKVSFTGSSETGRKVFASAAPTLKHLTLELGGNDPGIVLPDADPQ
                     AIAEYLFRSMFLLSGQGCCTLKRLFVHESIYQALTEALISCAQHQKLGDGFDPETTLG
                     PIQNRPQFARMQTTWEEIQEAKAPILYQGEVPSEGAGLFFPVTLLDNPPAEAAYVVRE
                     NFGPLRAVFKYKDLDEAVRLANGTPYGLGASVWGTDSESLDAVARRLDAGIVWINQHL
                     NLHPNIPFSGHKNSGIGIEFGEDGLKEFCNVQVIAN"
BASE COUNT          289 a          374 c          329 g          295 t
ORIGIN      
        1 gtctcgacct tgcggttcag gccgcaaagg cagcgtttcc cctttggtgc agcaagacct
       61 ggaacgagcc aagggatctt cttcgaaatg ccatggaaga gctcaaagct catacaagtg
      121 agctttgcac gatcctgaca gcggaaaacg gccggcctta tagcttggcg caattcgaga
      181 ccacatggat tttcgagacc tatgtcccgg cgttacttca gatgaaactg ccagattcga
      241 catggaatca aaccggtgtc gggcagatca cgaaacgtta tgttccactg ggtgtcgttg
      301 ccgcgatcag tccgtggaat cttccgttct tgctgtcatt cacaaaagtg cttccatctt
      361 tgctggccgg caacaccgtt gtcctgaagc ccgcggtttt tacccctctt acggtacttc
      421 gagccgccga ttacattcgt gagatacttc cgcccggcgt tttgaatgtg atcagcggag
      481 gggatgacct tggcccctgg atgacgtccc acccggactt ccagaaggtc tccttcacag
      541 gatcttcgga gaccggacgc aaggtcttcg catccgccgc cccaaccctg aaacacctga
      601 cccttgagct aggcgggaac gatcccggta tcgtacttcc ggatgccgat cctcaggcaa
      661 tcgccgagta cctgtttcga agcatgttcc tactcagtgg ccaggggtgt tgcactctca
      721 aacgcctttt cgtgcacgag agcatctatc aagctcttac ggaagcactc atatcctgtg
      781 ctcaacatca gaaactcggc gatggctttg atcccgagac caccctgggc cctatccaga
      841 accgccccca gttcgctcgc atgcaaacaa catgggaaga gattcaagag gcgaaagctc
      901 ccatcctcta tcaaggcgaa gttccatcgg agggtgccgg cctgtttttt cccgtcaccc
      961 tcctggataa tccgcctgcg gaagcggctt acgtagtccg agaaaacttt ggaccattgc
     1021 gggccgtttt caagtacaag gacctcgacg aagccgtccg gctggccaac gggactccat
     1081 atggccttgg agcctccgtt tgggggacag actctgagtc actggacgcc gttgcacgtc
     1141 ggctcgatgc cggaatagtt tggatcaatc aacacctcaa tctccatccc aacattccat
     1201 tcagcggtca taagaattca gggattggca ttgaattcgg cgaagatggg ctcaaagaat
     1261 tctgcaatgt gcaggtcatt gcgaaca
//
LOCUS       DATMKH010000046         2314 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4515373, whole genome shotgun sequence.
ACCESSION   DATMKH010000046 DATMKH010000000
VERSION     DATMKH010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4515373"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /locus_tag="VNV63_00535"
     CDS             <1..617
                     /locus_tag="VNV63_00535"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HXC61132.1"
                     /translation="HKIQRKNLLFGAILLLAYTIILIVSMLLSPASAPMSNAPLAAEK
                     EVTDQFIALVSRFWPAFSISFILSLTLGLFLTHRLAGPLYRFEQTIKAILSGDLSVRI
                     RLREKDEFQELALLLNQAVVEIEQRLRAIETKGHQLQERLDVVLAELEQQPQGQVIAQ
                     ELEPVQNLHNELMVLIKRFQLSSPPPDFARLEGLRPRSEHPDDR"
     gene            721..1704
                     /locus_tag="VNV63_00540"
     CDS             721..1704
                     /locus_tag="VNV63_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009381878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HXC61133.1"
                     /translation="MSKEITTKVRLQTGLDTSNLFGNFDSHLKMMEEAFRIHVSARGE
                     EVLITGEAERVRQAERFLADMGALIQEGYTLRPEDVQHALKARKEDSQASLKSLFHEA
                     IPISGKKQFVIPKSVTQRNYLEAIQKNDIVISIGPAGTGKTYLAMAMAVWALTKRQVN
                     RIILARPAVEAGEHLGFLPGDMYAKVNPYLRPLYDALYDMMEMDRVNRLAERGDIEIA
                     PLAYMRGRTLNDSFVILDEAQNATAEQMKMFLTRLGFNSKAVVTGDITQVDLPAERAS
                     GLIQIQQILAGIPGIEFVYFTERDVVRHKLVQEIIKAYERYEGRSGKGKKK"
     gene            1746..2186
                     /gene="ybeY"
                     /locus_tag="VNV63_00545"
     CDS             1746..2186
                     /gene="ybeY"
                     /locus_tag="VNV63_00545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HXC61134.1"
                     /translation="MKVWIRNRQRRIRVRMREVSASARKILLTLGCLKAELSLLLVND
                     AQIRRLNKQYRRINRPTDVLAFPMLEGGASPLRSQLLGDVVISLETAKKQAKKESHSL
                     EQEVKILLIHGILHLLGYHHEDSDEAAGLVERKTQAILEELERD"
BASE COUNT          545 a          585 c          655 g          529 t
ORIGIN      
        1 tccataagat tcagcgaaaa aacctgctgt tcggtgcgat tctgcttctc gcctatacga
       61 tcatcctcat tgtctcgatg cttctttctc cagcttcggc cccgatgtcg aatgcaccgc
      121 tggcagccga gaaagaagta acggaccagt ttatcgcgct ggtcagtcgc ttctggccgg
      181 ccttctcgat cagctttatt ctatcgctca ctttgggact ttttctcacc caccggttgg
      241 cgggtccgct ttaccgtttt gaacagacga ttaaggcgat cctgagcgga gatctctccg
      301 tccggatccg gttgcgagaa aaggacgagt tccaggagct agccctctta ctgaaccagg
      361 ccgtcgtcga gatagaacag cgcctccggg caatcgagac gaaggggcat cagctccagg
      421 agcgtctgga tgtggtcctg gctgagctcg agcaacaacc acagggtcaa gttatcgccc
      481 aggagttaga acctgttcag aatctgcaca atgagctgat ggtgttgata aagcggtttc
      541 aactgagttc tcctccacct gattttgccc gtttggaggg tcttcggccc agatccgagc
      601 atccggacga tcggtgagaa ggcgaacgtg atccatgcgt cgtcggattg accctgctcc
      661 gttgatactg atatactttt caaaggataa ggatgatata gagggagagc gattctttct
      721 ttgagtaaag agattacaac caaggttagg ctccaaacag gtttggacac ctccaaccta
      781 ttcggaaact ttgacagcca tctcaagatg atggaagaag ccttccgtat ccatgtttcg
      841 gcaagggggg aagaggttct catcacgggg gaggcggaac gagtccgaca ggcagagcgc
      901 tttcttgccg atatgggtgc gctgatccag gagggctaca cccttcgacc ggaagatgta
      961 cagcatgcac tcaaggccag gaaagaggat tctcaagctt cgctcaagtc tctgtttcat
     1021 gaggcgatcc cgatttccgg gaagaagcag ttcgtgattc ctaaatcagt cacgcagcga
     1081 aactatctgg aggcgatcca gaagaacgac atcgtgatca gcattggtcc cgccggcacc
     1141 ggcaagactt atctggccat ggctatggcg gtctgggcgc tgacgaaacg tcaggtcaac
     1201 cggatcattc tagctcggcc tgcggtggag gcgggagagc atttgggttt tcttccgggg
     1261 gacatgtacg ccaaggtgaa cccgtatctt cggccgctct atgatgccct gtacgatatg
     1321 atggagatgg atcgtgtcaa ccgcttggcc gaacgaggag atattgagat cgcaccgttg
     1381 gcttatatgc gcggccggac tctgaatgat tcattcgtga ttctggatga ggcccagaac
     1441 gccaccgcgg aacagatgaa aatgttcttg actcgtttgg gttttaactc gaaggctgtg
     1501 gtcacgggcg atatcaccca ggtagatctg ccggccgagc gggcttccgg tttgatccag
     1561 atccaacaga ttctggccgg catccccggc atcgagtttg tctatttcac tgaacgggat
     1621 gtagtccgcc acaaactggt ccaggaaatc atcaaggcct atgaacgtta cgagggtcgg
     1681 agtggaaaag ggaaaaagaa ataagtatct cgggacaaac cgggcaggtt atggagaggt
     1741 gatagatgaa ggtgtggata aggaatcggc agcggcggat acgagtacgg atgcgggaag
     1801 tctccgcctc ggccagaaaa atcctcctga ccctgggatg cctcaaggct gaactcagcc
     1861 ttctcctcgt caacgatgct caaatccggc ggttgaacaa gcagtaccgg cgaatcaatc
     1921 gtcctaccga tgtccttgct ttcccgatgc tggagggcgg tgcctctccg ctgcgttctc
     1981 aattgctggg cgatgtcgtg atctcactcg agacggcgaa aaagcaggca aagaaagagt
     2041 cccattctct ggaacaggag gtaaaaatcc ttttgatcca tgggatactc cacctcttgg
     2101 gttatcatca cgaggactcg gacgaggccg ccggactcgt agaacgaaag acccaggcca
     2161 tcttggaaga actggagaga gactaagcat ggtcgaaggt ttgtttcgtc gtttccgtca
     2221 ggcgttgtcc agaacccgaa aggcgttttc ctcacagatc ggagtgctcc tttcagggcg
     2281 acaccctctg gatgctcaaa cgctggaaga actg
//
LOCUS       DATMKH010000047         1114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3232741, whole genome shotgun sequence.
ACCESSION   DATMKH010000047 DATMKH010000000
VERSION     DATMKH010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3232741"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..955
                     /locus_tag="VNV63_00550"
     CDS             155..955
                     /locus_tag="VNV63_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HXC61135.1"
                     /translation="MNRVLLIFVLVGVAGCSSYTPDAGHQVVLVEKPWLIGHGGVNPD
                     PIATGRTYTAWSTDGIDVNMQPQKFETELPDTMTQDGVPITFHAIMVIKVTDAVNLIK
                     NFGPEWYKNNLEEPFKTMVRQAVRKRGMNETAISTTALDAIDAEIRDELVAFIKDKNL
                     PVMLVTMTVGKANPPDAVKNQRIETATQEQRIQTEKQKKLAEDQRQQAEQSRANADNA
                     YREAMHLSPEQFIQLETIKMQNDVCGQQGKANCTFIQNGTTPVYNLNK"
BASE COUNT          293 a          274 c          309 g          238 t
ORIGIN      
        1 cgcaaatttg ggcacggcgc gttctttctg ctaccgggcg ctaaggtgat cccgaggatc
       61 ttggtcttta agtttctgct gtatacccgc accgatggta tgtccaagct gatgaagaag
      121 gtttgcaaat tctagcagga aaagaggagg ccgcatgaac cgagtgctac tgatttttgt
      181 gttggttgga gttgccggat gcagttcgta tacgccggat gcaggacatc aggtcgtttt
      241 ggttgagaag ccatggttga tcggtcatgg cggcgtgaat cctgatccga ttgcaaccgg
      301 tcggacatat actgcctgga gcaccgacgg tatcgatgtc aacatgcaac ctcagaagtt
      361 tgagactgag cttcctgaca cgatgaccca ggacggagtc cccatcacct ttcacgccat
      421 catggtgatc aaagtgaccg atgcggttaa tctaattaag aatttcgggc cggaatggta
      481 caaaaacaat cttgaggagc cgttcaagac gatggtgcgg caggcagtcc ggaagcgcgg
      541 catgaacgag acggccatct cgaccacggc cttggatgcg atagatgcgg agatccgaga
      601 cgaactggta gccttcatca aagacaagaa cctgccggtg atgctggtga cgatgactgt
      661 cggtaaggcc aatccaccag acgcggtcaa aaaccaacgc attgaaactg ccactcagga
      721 gcagagaatc cagacggaaa agcagaagaa gctcgccgag gatcagcgcc agcaggcaga
      781 gcagagccga gctaacgccg acaacgccta ccgcgaagcc atgcacctgt ctccagagca
      841 gttcattcag cttgagacca ttaagatgca gaatgacgtt tgtggccagc aaggaaaggc
      901 gaactgcacc ttcatccaga acggaaccac tcccgtttac aatcttaaca agtaagggta
      961 cggagaaagg tcagatgagt gcaggcaaaa tttgccagag agcatggatt ccctacgccg
     1021 actccatgga gttcatcggc gtcttgcgtt cactcaatct cctgttcctt ttttggagaa
     1081 tgggcgacat aagtgacgcc tcgatctttt gatt
//
LOCUS       DATMKH010000048         1489 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_346858,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000048 DATMKH010000000
VERSION     DATMKH010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346858"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..623)
                     /locus_tag="VNV63_00555"
     CDS             complement(57..623)
                     /locus_tag="VNV63_00555"
                     /inference="COORDINATES: protein motif:HMM:NF014019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HXC61136.1"
                     /translation="MICPNCGLDQLEGSTCANCQATLPRPLTASEIKAMKAGFEKKEA
                     TVPPSAPPPIEFGLTDLEAGSPSQTKSASSPGIEQFQKVLVITSSIVEGRKIVRYGDP
                     LCCQSVVKLDGLDKFLAGIKDVSSLRNTPYGELFKKAEMILMSDLKIEAAKKGANAVI
                     GTSIHYEFCFKNAVMVHANGTVAYLDEA"
     gene            complement(620..1243)
                     /gene="purN"
                     /locus_tag="VNV63_00560"
     CDS             complement(620..1243)
                     /gene="purN"
                     /locus_tag="VNV63_00560"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HXC61137.1"
                     /translation="MSEKKLNLGVLVSGQGSNLRAILKACREGQLSASVGIVISDKKD
                     AQALEHARQFKVPELFLDPRASASRETYDQVILKELKKHSVELVILAGYMRIVTPVLI
                     QAYRNKIMNIHPSLLPAFPGLHAQRQALAYGVKVSGCTVHFVDEQVDHGPIISQMAVP
                     VLENDTEETLETRILAEEHKLYVQAIQLFAAGRLEFQDRKVFIKGPR"
     gene            complement(1236..>1489)
                     /locus_tag="VNV63_00565"
     CDS             complement(1236..>1489)
                     /locus_tag="VNV63_00565"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HXC61138.1"
                     /translation="FPKGCEAVIHRASWRVPEIFRQIQRDGLVDEEEMYRVFNMGIGM
                     ILVIAPSSVKAVINQVRQSGERASIIGEVRKGKPRVCYV"
BASE COUNT          353 a          426 c          333 g          377 t
ORIGIN      
        1 aacatagtga acaccaagtt ggatcacgtc ttccatactg tttcacctcc gcacttttaa
       61 gcttcgtcga gataagcgac agtgccgttg gcatggacca tcacggcatt tttaaaacaa
      121 aactcgtaat ggatcgaggt cccgataacc gcattggctc cttttttcgc cgcttcaatc
      181 tttagatcgc tcatcagtat catctcggcc tttttaaaaa gttctccata aggtgtgttt
      241 cttaaactcg agacatcctt gatgcccgct aaaaatttat ctagtccgtc gagcttgacc
      301 accgactggc agcagagcgg atctccgtag cggacgattt ttctgccttc cacgatagac
      361 gatgtaatca ctaaaacctt ctgaaactgt tcgatccccg gtgacgaggc ggacttggtc
      421 tggctcgggc tgccggcctc aagatctgta agaccgaact cgatgggcgg cggtgccgat
      481 ggcggaacgg tcgcctcctt tttttcgaag ccggccttca tcgccttgat ctcggaagcg
      541 gtcagcggtc taggaagcgt cgcctgacag ttcgcgcagg ttgatccctc aagctgatcc
      601 agtccgcagt tcgggcagat catcgcggcc ccttgataaa aacttttcgg tcttgaaatt
      661 ccaaccgccc cgctgcaaac aattggatcg cctggacata gagcttgtgc tcttccgcca
      721 agatacgcgt ctccaaggtc tcctcggtat cgttttctaa aacgggcacg gccatctgcg
      781 agatgatcgg tccgtgatcc acctgctcat ccacaaaatg gacggtgcag ccggacactt
      841 tcaccccata agctaacgcc tgccgctgcg catgaagacc cggaaaagca ggaagcagag
      901 aaggatgaat attcatgatc ttattccgat acgcctggat caagaccggc gtaacgattc
      961 tcatataacc cgccaggatc accaactcca cggaatgttt ctttagctct tttaagatca
     1021 cctgatcgta tgtctcccgg cttgctgagg ctctcgggtc taaaaaaagt tccggaactt
     1081 taaattgtct tgcatgttct aatgcctgtg catctttttt atccgaaatc acaatgccca
     1141 ccgacgcgga aagttgcccc tccctacagg ctttcaggat cgctctaaga ttagaacctt
     1201 ggccagagac caggacaccc aagttcagtt ttttttcaga cataacacac ccggggcttt
     1261 cctttccgaa cctctccaat gatggacgcg cgttctccgg attgcctgac ctgatttata
     1321 acagccttaa cggaagaagg cgctatgacg agaatcatcc cgatgcccat gttgaaaacc
     1381 cggtacatct cttcctcgtc tacaaggccg tccctttgaa tttgacggaa gatctccggc
     1441 accctccagc ttgcccgatg gatcactgcc tcacaaccct tcggaaaaa
//
LOCUS       DATMKH010000049         2317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_988495,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000049 DATMKH010000000
VERSION     DATMKH010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_988495"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="VNV63_00570"
     CDS             <1..296
                     /locus_tag="VNV63_00570"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HXC61139.1"
                     /translation="FVVPPGVWLTNLETVEPDSAVGGGVPTGSRPTQTLGGKQVKFIG
                     FTKSDAAMTQFIAALERSEHFTEIALIYSQRGAEPDSQQVNFEIAANLRTARF"
     gene            305..910
                     /gene="pilO"
                     /locus_tag="VNV63_00575"
     CDS             305..910
                     /gene="pilO"
                     /locus_tag="VNV63_00575"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HXC61140.1"
                     /translation="MEDGQVLKENWIRALDFSRLAPREKILLIATMVIVLFAAWNYLY
                     LPKSRQVSDARTQIALAQTEIAQLSAQLPEIRKKEEELRLIQEKGEATGAFSSKIGDL
                     MPGGSRLSSLLEEMTRLARMQRVEFISIRPEAIEDRGNYLQLSLRIDMKSRFREMGDY
                     LLMLENLPRAIRVKEVKVETNAGINPDILAHLQALTYISKE"
     gene            931..1287
                     /locus_tag="VNV63_00580"
     CDS             931..1287
                     /locus_tag="VNV63_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61141.1"
                     /translation="MATLLILLGLTSSSFAQSLSDINLDWLERQEKYHQWGRDPFVIP
                     SASSGEGSGKEGGLRLSAIIYRKNGGVAIINNRILRKGDRIDGKEVARILEDRVILRD
                     SSGDRELQVNKFSFGR"
     gene            1295..>2317
                     /locus_tag="VNV63_00585"
     CDS             1295..>2317
                     /locus_tag="VNV63_00585"
                     /inference="COORDINATES: protein motif:HMM:NF015891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HXC61142.1"
                     /translation="MRHFDFKIVIFLFLILGLMLMGMGMILVRAEPLSGGTISVTREP
                     GIARGPVFSMEFRDADLKDVLRAIGQENNLNIIVGEDVQGKLTLSFQKVALKDALDAI
                     FRNYNLASFQEGSVLRVVKSPFLEGEENLMTKMIPMNYTKAKDIAITVKGLLSRKASL
                     AVDERTNTLLVRDVPDNVEKILDIVKGLDTKTPQILIEARIVEATTSYARELGIQWGG
                     KAQQVTGTPFTLTGGSTQTASIGPGSPTTGGVGLSGNNFIVNFPATLEQGFGSALGFT
                     IGNLTGTNQLDIQLSAMEDSGKGRILSNPKILALDNKEAKISSGYEILIPVTAILGIG
                     TSATSGA"
BASE COUNT          566 a          556 c          685 g          510 t
ORIGIN      
        1 gcttcgtcgt cccgccgggg gtatggttga caaacctcga gacggtcgag cccgactcag
       61 cggttggagg cggagtacca acaggctcac ggccgactca gaccttgggg ggtaaacagg
      121 tcaagtttat cggatttacc aaatctgatg ccgccatgac gcagttcatc gcggcgctgg
      181 agcgatcgga gcatttcacg gaaatagcgc tgatctattc ccagcggggc gcggagcccg
      241 attctcagca ggtaaatttt gagattgccg cgaatttgag aacggcgcgg ttttaaaaaa
      301 tcaaatggaa gatggacaag tgttaaaaga gaactggatc agggcacttg atttttcgcg
      361 gctggcgcct cgggagaaaa ttctcttgat cgctaccatg gtaattgttt tattcgctgc
      421 ctggaactat ctctatctcc cgaaatcgag gcaggtgagc gatgcccgaa cgcagatcgc
      481 tttggcccag accgagattg cccagctttc ggctcagctt cctgagatac gaaaaaagga
      541 ggaggagctt cgattgatcc aggagaaagg tgaagcgacc ggtgcgttct cgagtaagat
      601 cggcgatctg atgcccgggg gaagccggct ttcatctctg ttagaggaga tgacccgcct
      661 cgcccgtatg cagcgggtcg agtttatttc gattcggccg gaggcgatcg aggatcgcgg
      721 aaattatttg caattgtcgc tccggatcga tatgaaatcc aggttccgag agatgggaga
      781 ctatcttctg atgctggaaa atctccctcg cgcgatccgt gtcaaagagg taaaggtgga
      841 gacgaacgcg ggcattaacc cagacatcct cgcccatctt caggcgttga cctatatcag
      901 caaggaatga gcatgagaat aataatctgg atggcaacac ttcttatcct gctgggtctt
      961 acgagctcca gctttgccca gtccttgtct gatatcaatc tggactggct ggagcgccag
     1021 gagaaatatc accagtgggg tcgggatcct tttgtcattc catcagccag ctcgggggaa
     1081 gggtcgggta aggagggcgg tttgaggctc agcgcgatta tctatcgaaa gaacggcggc
     1141 gtggccatta tcaataaccg catccttcga aagggggatc ggatcgatgg gaaggaagtc
     1201 gcccggatct tagaggaccg ggtgatctta agagacagca gcggagatcg agagctgcag
     1261 gtcaacaaat tcagctttgg gaggtagaac gatcatgcga cattttgatt tcaagatcgt
     1321 gatcttcctg ttcttgatcc tcggcctgat gttaatgggg atgggaatga tcctagtgcg
     1381 ggccgagcct ttgagcggtg ggacgatctc agtcacccgc gagccgggta ttgcgagagg
     1441 tccggtcttc tcgatggagt ttcgggatgc ggacctgaaa gatgtccttc gggcgatcgg
     1501 acaggagaac aatctcaata tcattgtggg agaggatgtc cagggaaaac tgacactgag
     1561 ctttcagaag gtcgccttaa aggatgcgct cgatgccatt ttcagaaact ataatctcgc
     1621 ctcctttcag gaaggaagcg tcctgcgtgt ggtcaaatca ccgtttctcg aaggagagga
     1681 aaacctgatg acgaagatga ttccaatgaa ttacaccaag gccaaagaca tcgcgattac
     1741 ggtcaaaggg ctcttaagcc ggaaggccag cctcgcggtg gatgagcgca cgaatacact
     1801 gctcgtgcgg gatgtgcccg acaatgtcga gaagatctta gacattgtga aaggcctgga
     1861 cactaagacg cctcaaatct tgattgaggc acggatcgtg gaggccacta cgagttacgc
     1921 acgcgaactg gggattcagt ggggcgggaa ggcccaacaa gttactggca ctcctttcac
     1981 gctcaccggc ggctctacgc aaacagcctc gatcggaccg ggcagcccaa cgaccggcgg
     2041 tgtgggtctg agcggaaata atttcatcgt taattttccg gccaccttgg aacaaggttt
     2101 cgggagtgcg ttgggtttta caatcgggaa tctgaccggc acaaaccaac tcgatatcca
     2161 gctctcggcc atggaggaca gcggtaaggg acgaattctt tccaatccca agatcctggc
     2221 cttggacaat aaagaggcca agatatcgag cggctatgag atcctgatcc ctgtcaccgc
     2281 aatcttgggg atcggcacgt ccgcaacctc gggagcg
//
LOCUS       DATMKH010000050         1311 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2271321, whole genome shotgun sequence.
ACCESSION   DATMKH010000050 DATMKH010000000
VERSION     DATMKH010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2271321"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="VNV63_00590"
     CDS             complement(<1..401)
                     /locus_tag="VNV63_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HXC61143.1"
                     /translation="MGKDTLVDFLVAKGFNKDDLKPNSKLTDDMYRSLQQEFQGDKVA
                     KIKSDQIDLPKGSLEAKKKKDEEPQPVKKEIPVVKKAPVKEEVPIPEEPVAPIIAAAP
                     PPAVDHAPAEAEITKIEAPELEGPRVIDKID"
     gene            complement(506..>1311)
                     /gene="nusA"
                     /locus_tag="VNV63_00595"
     CDS             complement(506..>1311)
                     /gene="nusA"
                     /locus_tag="VNV63_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HXC61144.1"
                     /translation="WKKEILLLDEEGSELILPKSEQIPQDYFKKGENIRAVVRKVELK
                     NNSPVIILSRTSPLFLSKLLEIEVPEIFDGLIVIKKIVREPGERAKVAVESYDDRIDP
                     VGACVGMKGSRIHGIVRELKNENIDVINWTNNTQLLIQRSLTPSKITNMELDSEKAHA
                     NVFLKPDQVSLAIGRKGVNIKLAQELTGYTIDVFRDNEGEPEEFDIDLEEFADEIETW
                     VIDELKRIGCDTARSVLELTADELERRTDLERETIDDVRRVLKEEFEKG"
BASE COUNT          242 a          357 c          316 g          396 t
ORIGIN      
        1 aaatcgatct tgtcgataac cctggggcct tccaattcgg gggcttctat cttggtgatc
       61 tccgcttctg cgggtgcatg gtccacggcc ggcggcggtg ccgcagcgat aatcggggcc
      121 acgggttctt cgggaatagg cacctcttcc tttaccggcg cttttttcac caccggaatc
      181 tctttcttaa ccggttgagg ctcttcgtct ttcttctttt tggcttcgag gcttcccttt
      241 ggcaggtcga tctgatcgct cttgatcttt gccactttat ctccctggaa ttcctgttgt
      301 aaggagcgat acatgtcgtc cgtcagcttt gaattaggct tcagatcgtc cttattgaat
      361 cccttagcca ccaaaaagtc cacgagggta tctttcccca cgttgaactc cttggccgct
      421 gccattaagc gtaatgatgt cgttgcttct gccatatttg tgtaaaaata aaactatttg
      481 gccgttatct attttcccgg ccatattaac ctttttcaaa ttcttccttc aataccctcc
      541 gcacatcatc gatggtttct ctttccaggt ccgtccgtct ttccagctca tccgctgtga
      601 gctccagtac gctccgggcg gtatcgcagc cgatcctttt gagttcgtcg atcacccagg
      661 tctcgatctc atctgcaaat tcttcgaggt cgatatcgaa ttcttccggt tcaccttcat
      721 tatcacggaa tacgtcgatc gtataaccag tcaactcctg tgccagtttg atattcaccc
      781 ctttacgtcc aatggccagg gagacctggt cgggctttaa aaagacattc gcatgcgcct
      841 tttcgctgtc cagctccata ttggtaatct tggatggcgt aagggaccgc tggataagca
      901 gctgggtatt gttcgtccag ttgatcacgt cgatgttctc gttcttcaac tcgcggacga
      961 tgccatggat gcggctgcct ttcatgccca cgcaggcgcc taccgggtcg atccggtcat
     1021 cgtaggattc cacggcaact ttggcgcgtt ccccgggttc gcggacgatc ttcttgatca
     1081 cgatgaggcc gtcgaagatc tcagggactt cgatctccag gagtttggac aggaagagag
     1141 ggctggtccg ggacaggatg atcacggggg agttgttctt caattccacc tttctgacaa
     1201 cggcgcggat attctctcct tttttgaagt aatcctgggg gatctgttcg gatttgggaa
     1261 ggatcagctc gctaccttct tcgtcgagca ggaggatctc ttttttccat a
//
LOCUS       DATMKH010000051         1052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2271326, whole genome shotgun sequence.
ACCESSION   DATMKH010000051 DATMKH010000000
VERSION     DATMKH010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2271326"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..627
                     /locus_tag="VNV63_00600"
     CDS             166..627
                     /locus_tag="VNV63_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010569183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HXC61145.1"
                     /translation="MTTSTRTIEVKVERTICAPPQEVFDAWLDPKIPGNPWNAAEKFI
                     LDPKVDGLLYWRLRGISHYGRFTEMERPVRMRHTWVSPNTLGKESTVTVAFQKHGEDT
                     LMTLVHSDLPDTDEAKGHERGWNYFLDILREQFGSGSRKDYRWEEAHPGGE"
     gene            767..>1052
                     /locus_tag="VNV63_00605"
     CDS             767..>1052
                     /locus_tag="VNV63_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXC61146.1"
                     /translation="MSSHSGNAKKIRGYAPVNGLKIYYEIEGTGDPLVFIPPAFGFAG
                     LKSFPTLAQNHSIITVDLQGNGRTADIPDRPISIEQYAKDVVDLLKYLGIA"
BASE COUNT          272 a          264 c          308 g          208 t
ORIGIN      
        1 acttgtcgag cgccaaagac aggggcgaga ggtcatgatt tcttttcgag cggagccgct
       61 gcgaatggta tcgggaaggg tacatgagta cgagcaattc tggaacgaac gcctcgatca
      121 ctttgagaag catttcaaag acaaaagggc gaaggagaaa aagaggtgac gacttcaaca
      181 aggactatcg aagtgaaggt tgagagaaca atatgtgctc ctccgcagga ggtattcgat
      241 gcgtggctgg atcctaagat cccaggcaat ccatggaatg ccgccgagaa gttcatcctg
      301 gatccgaaag tagacgggct cctttactgg cgcctccgcg gcatctcgca ttacggccga
      361 tttacggaga tggagcggcc agttcggatg cggcatacgt gggtgtcgcc gaacactctc
      421 ggaaaggaat cgacggtgac ggtcgccttt cagaagcacg gagaagacac gctgatgact
      481 ctcgtgcatt cagatcttcc agacacggac gaggcaaagg gacacgagag aggctggaac
      541 tactttttag atattctccg tgagcaattc ggaagtggtt cacgcaagga ttaccgctgg
      601 gaagaggctc atccgggggg ggaatgagaa agctgagcga cctcaaggca aggggaattc
      661 gccgattctt cggggcggta cttctgcctc tgctacttgc tactcaagcg atgggcggaa
      721 actgcgccac ccaggtgcaa cccagacctc agcgaaagga accgtggtga gctcacacag
      781 cggcaatgca aagaagatac gagggtacgc gcccgtcaac ggcctgaaga tatattacga
      841 aatcgaggga accggcgatc cgctcgtctt tatccctccc gcctttggat ttgccgggct
      901 gaaatcattc cctacgctgg ctcagaacca ttcgatcatt acggtcgatc ttcagggcaa
      961 tggccggaca gcggatatcc cggaccgccc catttccatc gaacaatacg ccaaagacgt
     1021 ggtcgatttg ctgaagtatc ttggcatcgc aa
//
LOCUS       DATMKH010000052         2807 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3554081, whole genome shotgun sequence.
ACCESSION   DATMKH010000052 DATMKH010000000
VERSION     DATMKH010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3554081"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..144
                     /locus_tag="VNV63_00610"
     CDS             <1..144
                     /locus_tag="VNV63_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005770149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HXC61147.1"
                     /translation="VRGDTATGGAKQAKLESGATIKVPLYIEEGEIIKIDTRTGTFVE
                     RVR"
     gene            154..618
                     /gene="accB"
                     /locus_tag="VNV63_00615"
     CDS             154..618
                     /gene="accB"
                     /locus_tag="VNV63_00615"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HXC61148.1"
                     /translation="MNIKELKELIDLLKDTEVTELEIEKAGVKVRIKKGRTVLSGVAD
                     HVDGSAVASVEKKTAMPETPEVGEKPVDSGIITVTSPIVGTFYRSSSPDALSYVEIGD
                     VVKKGQVLCIIEAMKLMNEIESEVDGKVVEILLENAQPVEYGEPLFRIEPLS"
     gene            691..2052
                     /gene="accC"
                     /locus_tag="VNV63_00620"
     CDS             691..2052
                     /gene="accC"
                     /locus_tag="VNV63_00620"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HXC61149.1"
                     /translation="MFKKILIANRGEIALRVIRACKELGIKTVAVYSEVDSGSLHVRF
                     ADESICIGPADQALSYRNVPNILSAAEITGAEAIHPGYGFLAENAHFAEVCGSVGIRF
                     IGPSPENIATMGDKAKAIEIMAQRGIPVLPGSRGIVEQEKAALEVAKKIGYPVIIKAA
                     AGGGGRGLRVVNREEDLIQAFQAAQAEAKTAFGDEGVYLEKYFLEPRHIEVQILADER
                     GRVIHLGERDCTVQRRHQKLIEESPSPVVDEHLRREMGAMAVEVARAVSYRNAGTIEF
                     LLDKDRNFYFMEMNTRIQVEHPVTEMITGVDLIKEQIKIAAGRPLSYKQHDVKFRGHS
                     FECRINAECPEKFTPSPGLITAYSVPGGPGVRVDSAVYAGYTVPPHYDSMIAKLIIHS
                     DSREESILRMLRALEEYIVEGIKTSIPLYKRIFEDANFQKGRYSTNFLERFLASHESP
                     GNL"
     gene            2030..2731
                     /gene="thiE"
                     /locus_tag="VNV63_00625"
     CDS             2030..2731
                     /gene="thiE"
                     /locus_tag="VNV63_00625"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HXC61150.1"
                     /translation="MNPPATSDPSESLSTASRSGLYLITDTAIIRGLPHQEAIQKAIQ
                     GGLKFLQYREKSLTPSISYEVAMVQRRIAQEAGVIFIVNDDVSLALAVEADGVHLGQE
                     DLPIREARKILGGQSLIGVSTHSVQEALAAEKDGADYIGLGPIYHTTTKQTRPPLGCG
                     IIREVKEKVRIPIFAIGGINLINLKEVLEAGATGVALISAILTSPDIAQATRTFLETI
                     SSVKMAHPGPHREAQ"
BASE COUNT          672 a          688 c          828 g          619 t
ORIGIN      
        1 gttcgcgggg ataccgccac cgggggggcc aaacaggcta aactcgaaag cggcgctacg
       61 atcaaggtac cactttatat cgaggaggga gagatcatta aaatcgatac cagaaccggc
      121 acctttgtgg aacgggtcag ataacagcca gcgatgaaca tcaaagaact aaaagaattg
      181 attgatctgt tgaaggatac cgaggtcacg gagctggaaa tcgagaaggc cggtgtcaag
      241 gtcaggatca aaaaaggccg gacggtttta tccggggttg cggatcatgt ggatggttct
      301 gccgttgcct cggtcgagaa gaagaccgcg atgccggaga cgccggaagt tggggaaaaa
      361 cccgtcgaca gcgggatcat caccgtcaca tcgcccattg tcggtacctt ctaccggtcc
      421 tcctcgcccg acgcgctttc gtatgtcgag atcggtgacg tggtgaaaaa gggacaggtt
      481 ttgtgcatta ttgaagcgat gaagctgatg aacgagatcg aatccgaggt ggatggcaaa
      541 gtggttgaga tcctgcttga gaacgcccag ccggttgagt atggcgaacc gctcttccgc
      601 atcgagccgc tgtcctaggc ggggcaggtc tcatcgggat tattttttgt ttatcttaac
      661 tcccatcgat cgtccttcgg ggagggaatc ttgtttaaaa aaattctgat cgccaaccgt
      721 ggagagatag ccctgcgagt cattagggcc tgcaaagagc tcggcatcaa gacggtcgcg
      781 gtctattccg aggttgatag cggttccctc catgtccgct ttgcggatga gagcatttgc
      841 atcggtccgg ctgatcaggc cttgagctat cggaatgttc ccaatatcct aagcgccgcc
      901 gaaatcacgg gggcggaggc catacacccc ggttacggtt tcctcgccga gaacgcacat
      961 tttgcagagg tctgtggatc ggtcggtatc cggtttatcg gtccctcgcc ggaaaatatt
     1021 gccacgatgg gggacaaggc caaggcaata gagatcatgg cgcagagggg gatccctgta
     1081 ctgccgggga gcagaggcat cgtagagcaa gagaaggcgg cgctggaggt tgcaaaaaaa
     1141 atcgggtatc ctgtcattat caaggccgct gccggaggag gagggcgggg cttacgggtg
     1201 gtgaaccgtg aagaggatct gatccaggca tttcaagcgg cccaggccga agcaaagacg
     1261 gcatttggag atgaaggcgt gtatcttgaa aaatactttc tggaaccacg ccacattgag
     1321 gtccagatct tggcggatga gcgcggtcgg gttatccatc tgggcgagcg ggactgcacg
     1381 gttcaacgtc gccaccagaa gctgatcgag gaatccccct cgccggtcgt ggatgagcat
     1441 ctccgtcgtg aaatgggggc gatggcggtt gaggtggccc gggccgtaag ttatcggaat
     1501 gcaggcacga tcgagtttct cttagacaag gacagaaatt tttattttat ggagatgaac
     1561 acgcgcattc aggtcgagca tccggtgacg gagatgatta ccggtgtgga tttgatcaaa
     1621 gagcagatca agattgcggc aggccgcccg ctcagttaca aacagcatga tgtcaagttc
     1681 cgcggccaca gctttgaatg ccgcatcaat gcagaatgtc cggagaagtt tacaccgagt
     1741 cccggtttga tcaccgccta ttcggttccg ggcggacccg gcgtcagggt agacagtgct
     1801 gtgtatgccg gttacacggt tcctcctcat tacgattcga tgatcgccaa gctcattatt
     1861 catagtgata gccgggaaga gtccattctg cggatgctcc gggcgttgga agagtatatt
     1921 gtggagggca tcaagacctc tattcccctt tacaaacgca tcttcgaaga tgccaacttc
     1981 caaaagggac ggtactcaac gaattttctg gaacggttcc tggcgagtca tgaatccccc
     2041 ggcaacctct gatcctagcg aatccttatc aaccgcaagc aggtcgggcc tttacctcat
     2101 cacagacacc gcgattatcc gtggtcttcc gcaccaggag gcaattcaaa aggcgatcca
     2161 gggcggctta aagtttctac aatatcgaga gaaaagcctc accccttcta tctcttatga
     2221 agtggccatg gtgcagcgcc gtattgcaca ggaagccggt gtcatattta tcgtgaatga
     2281 cgatgtttca ttagctctgg ctgtcgaggc ggatggtgtt catttgggac aggaggatct
     2341 accgatccgg gaagccagaa agatactggg gggtcaaagt ctgatcggtg tctcgaccca
     2401 ttcggttcaa gaggcgttgg ccgccgaaaa agatggtgcg gattacatcg gcctgggccc
     2461 gatttaccat accacgacaa aacagacgag gccgcccctg ggctgcggta taatacgtga
     2521 ggtgaaagaa aaagttcgga ttcccatctt tgccattggt ggaatcaacc tgatcaatct
     2581 taaagaggtt ctggaggcgg gggcgacagg tgtggccctg atctcggcca ttttgaccag
     2641 ccccgatatc gcacaagcca cccggacttt tcttgagacc atttcttcgg taaaaatggc
     2701 ccatcccggg ccccaccggg aggctcaata gtcccaccgt gtgattcgat agctccgtcg
     2761 ggattattag cgggtcccat cgggattatt cgacggtcac atgagat
//
LOCUS       DATMKH010000053         2394 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4518052, whole genome shotgun sequence.
ACCESSION   DATMKH010000053 DATMKH010000000
VERSION     DATMKH010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4518052"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..900
                     /gene="murD"
                     /locus_tag="VNV63_00630"
     CDS             <1..900
                     /gene="murD"
                     /locus_tag="VNV63_00630"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HXC61151.1"
                     /translation="LSEAADSKWDFIVAEVSSFQLERVKAFRPKIGAYLNLTPDHLDR
                     HGTLKAYGMLKARLFENQGPDDFAVLNADDPEVCRVGRSLRSHVVWFSRVRPVDPGIY
                     LDDPDIVSTIAKKRSVFKRSDLRLKGVHNLENVLAAVAVAQLAGCDDNAIAETLKQFS
                     GLEHRLEWIREKDGVIYINDSKGTNIGAAIRSLESFSEPIIWIAGGREKGTDFSALRE
                     IVQSRVKEAIFYGEARVKLKKALDGAARIQEVKKLSEAVALAASIAIPGDVVLLSPAC
                     ASFDQFRDFEERGEVFKDEVRRL"
     gene            901..2007
                     /gene="ftsW"
                     /locus_tag="VNV63_00635"
     CDS             901..2007
                     /gene="ftsW"
                     /locus_tag="VNV63_00635"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HXC61152.1"
                     /translation="MGTEKIDKTVIIVTLLLVSIGVVMVYSASAVLAVRHYGDSFYFF
                     KRQLVWAAMGVLTLMIFAMIPFSVWHRFSTPIFLLTCGLLLIVLIPSMGMEANGSRRW
                     LKLGGFSFQPSEAAKLAIVFYLARYLSRKGSQLQDFFHGFSPPVVMVGVVLLLVMAEP
                     DLGTAVLIGVVGGCLLFVGGARWRHLLTLAVLLIPVVAGLILGTGYRRQRLMAFLDPW
                     NHQADGGFQAVQSFLALGGGGPLGTGLGEGRQKLFFLPEPHTDFIFSVLGEELGFLGA
                     AAVLILFLILISRGLMISIRANQPFGYYLGLGISLMLGIQVMLNIGVVTGLLPTKGLT
                     LPFLSYGGSSLVTSLAEVGILLNVSRNREAMRRG"
     gene            2004..>2394
                     /locus_tag="VNV63_00640"
     CDS             2004..>2394
                     /locus_tag="VNV63_00640"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXC61153.1"
                     /translation="MRVMIAGGGTGGHVYPGIALAREFQRQAVGTEILFVGTEKGLEA
                     RLLPREGFKLSTIRVRGLIGKGRVGGLSTLLRLPIACGDAAALLSRFKPDLVIGTGGY
                     ASGPVILAAWLMQKKRVILETNLMPGLT"
BASE COUNT          458 a          576 c          749 g          611 t
ORIGIN      
        1 ctctccgaag cggcagattc aaagtgggac tttatcgtag ccgaggtgag cagctttcag
       61 ctcgaacggg tcaaggcgtt tcgacccaag attggggctt atctgaatct cactccggac
      121 cacctggatc ggcacggtac actgaaggct tacgggatgc tcaaggcccg gctctttgaa
      181 aaccagggcc cggatgattt tgcggtcctg aatgcggatg acccggaggt ctgccgggtg
      241 ggccggtctc ttcggtcaca cgtcgtctgg ttcagccgag tgcggccggt tgatcctgga
      301 atttatctcg atgacccgga cattgtatcg acaatcgcga aaaagagatc ggttttcaaa
      361 agaagcgatc ttcgattaaa aggggttcac aatctagaga atgtcttagc cgccgtagcg
      421 gtcgcccaat tggcaggttg tgatgataat gcgattgctg aaacgctcaa gcagttttcc
      481 ggcctggagc atcggttgga atggattcgt gaaaaggacg gcgtcatcta tattaatgac
      541 tcgaagggga caaacattgg ggcggcgatc cggtctttgg agagcttttc tgaaccgatc
      601 atctggattg caggcggacg tgagaaaggg acagatttct ctgctctgcg cgagattgtt
      661 caatcccggg tcaaagaggc gatcttctac ggtgaggccc gtgtcaagtt gaaaaaggcc
      721 ctggatggtg ccgcccggat tcaggaagtc aagaaactct ccgaggcggt tgccttggcg
      781 gcctcgattg cgatcccggg agatgtggtg ttgctctcac cggcctgtgc gagttttgat
      841 cagttccgcg acttcgagga acggggggaa gtttttaaag acgaggtcag gcgtttatag
      901 atgggaacag agaaaattga taaaacggtt atcatcgtca cgttgctgct tgtatccatc
      961 ggggtcgtga tggtgtatag cgccagtgcg gtcctggccg tcaggcatta cggggattcc
     1021 ttttatttct tcaagcggca gcttgtctgg gccgcgatgg gtgtattgac tctgatgatc
     1081 tttgccatga ttccattctc ggtctggcac cgcttctcaa cgccgatttt tcttttaacc
     1141 tgcggtctgc tccttatcgt attgatcccc tcgatgggga tggaggccaa cgggtcccgg
     1201 cgatggttga aattgggcgg tttttctttt cagccgtcgg aggcggccaa gcttgcgatc
     1261 gtcttctatc ttgcccgcta tctcagccgg aaagggtcac agctgcaaga ctttttccat
     1321 ggtttttccc cgcccgtggt catggtaggg gtagtgcttc ttttagttat ggcagagccg
     1381 gatctcggaa ccgccgtgct gatcggagtt gtgggtggat gcctgctctt tgtgggcggc
     1441 gcccggtggc gtcacctgct gacgctggct gtcttgttaa ttccggtcgt ggcgggcctg
     1501 atcttgggaa ccggttatcg gagacaacgg ctcatggctt ttctcgatcc atggaaccac
     1561 caggcggacg gcggttttca ggcggttcag tccttcttgg ctttgggcgg cggaggaccg
     1621 ttgggaaccg gactcggcga gggccgtcag aagcttttct tcttgccgga gccgcacacc
     1681 gattttattt tctctgtcct gggcgaagag ctcggatttt tgggcgcggc ggcggttcta
     1741 attctttttt tgatcctgat cagccgcggc ctaatgattt cgattcgtgc aaaccagccg
     1801 tttggttact atctggggct cggaatcagt ctcatgctgg gaatacaggt gatgctgaat
     1861 atcggcgttg tgaccggtct tctgccgacg aagggattga cgctgccgtt cttgagttat
     1921 ggcggttcgt cgctggtgac cagtctggcg gaagtcggaa tcctgctgaa tgtttcaagg
     1981 aatcgtgaag cgatgaggag aggatgaggg tgatgatcgc tggcggagga accgggggac
     2041 atgtctatcc cggaatcgcc ttggcccgtg agtttcaaag acaggcggta gggactgaga
     2101 tactgttcgt cggaaccgag aagggtttag aggcgcggct cctcccgcgt gaaggattta
     2161 agttaagcac gattcgggtc cggggcctca tcgggaaggg tcgggtggga ggtctatcaa
     2221 cgttgctccg gcttccgatc gcctgcgggg atgccgctgc actgctctcg cgttttaaac
     2281 ccgacctggt cattggaacg ggaggctacg cctccggacc ggtgattcta gcggcctggc
     2341 tgatgcaaaa aaaacgggtc atcctcgaaa ccaatctgat gcccggtctc acca
//
LOCUS       DATMKH010000054         3354 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1310353, whole genome shotgun sequence.
ACCESSION   DATMKH010000054 DATMKH010000000
VERSION     DATMKH010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3354
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1310353"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="VNV63_00645"
     CDS             complement(<1..721)
                     /locus_tag="VNV63_00645"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF016838.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DmsC/YnfH family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HXC61154.1"
                     /translation="MAYLADVTSLVLIQIAIGGTILLPILPESVIGLGFFRFNSLLFL
                     ALFCAGLALMPDVPFREVGPSILFALSLLIYHLLSWTKAAFARRILLLVTALTGVVVL
                     LMTPLALQAHLAIRWKEILMPLSFLISSLLLGSVVDGMILGHSYLNIPALPTKLLMRF
                     SILFLVGLVFQGGFTLFSILSGMKTDLVRSALFLQSFEGLFLWMRVAIGIIGPLILAV
                     MILETVRIRSTQSATGLLYIAM"
     gene            complement(835..1161)
                     /gene="erpA"
                     /locus_tag="VNV63_00650"
     CDS             complement(835..1161)
                     /gene="erpA"
                     /locus_tag="VNV63_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HXC61155.1"
                     /translation="MITVTEQAGKKVQSLLEAEQKQTTGGLRIYVSGGGCHGFQYGMS
                     LEEQSRDEDTVIESGGVKVFVDPHSLPVLQGLEIDYLDSLQGAGFAIKNPNAKSSCGC
                     GSSFSA"
     gene            complement(1201..1518)
                     /locus_tag="VNV63_00655"
     CDS             complement(1201..1518)
                     /locus_tag="VNV63_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HXC61156.1"
                     /translation="MPKVTFQTIGKSGEVAVGTNLLQAAEQFGYELRHDCGGFANCST
                     CRVVIESGMENLSEVEFDEENMLDVAELVTPHRLACQAKVHGNVVVRTPDEEAIQDVR
                     ELK"
     gene            1655..2221
                     /gene="pyrE"
                     /locus_tag="VNV63_00660"
     CDS             1655..2221
                     /gene="pyrE"
                     /locus_tag="VNV63_00660"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HXC61157.1"
                     /translation="MDKRERASFRQQLLKKLRDSFVYSNEPTFTLSSGRKSHFYIDCK
                     KVTLDAEGAFLVGQLILDAVRDLEVDAIGGMTLGADPIATAAAVLSYGGEQEIPAFIV
                     RKEPKPHGTSAYIEGNVARESQVVVVDDVLTGGRSTERTIRILEEAGCKIIKVIALVD
                     RKEGGRERLEALGYTVESLFTVEDLLKS"
     gene            2308..2502
                     /locus_tag="VNV63_00665"
     CDS             2308..2502
                     /locus_tag="VNV63_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61158.1"
                     /translation="MASKKPEEAKGQKKPEEAKKEKKPTPLTIIQVPNDKEELESEMQ
                     DLFNEDKVEHIQGNIEPEAD"
     gene            complement(2518..2919)
                     /locus_tag="VNV63_00670"
     CDS             complement(2518..2919)
                     /locus_tag="VNV63_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61159.1"
                     /translation="MDIKRVKFFSIGTQDKPGELARFAKRMMEASVDMAGVWGFAKGQ
                     GKAEIIAIPKDPDKFKKAAQASGWTVKEGPCFHLTGEDRVGALSDTLDRVAQEGINLH
                     AVDAIALGGKYAAYVWTEEKDVERLAKILKG"
     gene            complement(2999..>3354)
                     /locus_tag="VNV63_00675"
     CDS             complement(2999..>3354)
                     /locus_tag="VNV63_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61160.1"
                     /translation="GAVLLVFLGSLSNQIPPVLFTEIGLWLLVLGLIEGVPTGFYYHL
                     VLYRILGPRSQLPPRWWMSPQQYHVHLDEKERRVVRRWFLLGGIGFIFSVIGGALVFL
                     GLISGFQQGTIKLGR"
BASE COUNT          910 a          891 c          837 g          716 t
ORIGIN      
        1 gcattgcgat atataaaaga ccggtcgccg actgagtgga ccggatccgg accgtctcaa
       61 ggatcatgac cgccaagatc aaaggcccga tgatcccgat cgctacccgc atccagagaa
      121 agagcccttc gaagctttga aggaataaag ctgagcgaac cagatcggtt ttcatcccgc
      181 tgaggatgct aaacagcgtg aagccgcctt gaaagaccag gccgactaaa aagagtattg
      241 aaaatctcat caggagcttc gtcggaagcg ccgggatatt gagataagaa tgcccgagga
      301 tcattccatc cacgaccgag cctagaagca aactcgagat caggaagctc agcggcatca
      361 ggatttcctt ccatcgaatc gccaggtgag cttgcaatgc cagtggtgtc atcaataaca
      421 ccacaacacc tgtgagcgcc gtgacgagca gtaagatgcg gcgcgcaaac gctgcttttg
      481 tccaggaaag aagatgataa atcaggaggc tgagggcgaa taggattgaa ggaccaactt
      541 cgcggaacgg gacatccggc atgagggcaa gtcccgcaca aaataacgcc agaaagagga
      601 ggctgttgaa tcgaaagaaa cccaatccaa tcaccgattc cggtaagatc ggtaacagga
      661 ttgtgcctcc gatcgcaatc tggatcaaaa caagtgaagt gacatcggcc agataagcca
      721 ttgggtctta aagatccttt cagacagggg aaaaaacaca ggcacaggga aataccctgt
      781 gcccgtgttt aataaatgag atgttagctt tctagcctgc tggcagccag tcgcttacgc
      841 gctaaaggaa ctgccgcacc cgcagcttga tttggcattt ggatttttaa tcgcgaagcc
      901 agcaccctgc agactatcaa ggtagtcaat ttccagcccc tggagaacgg gaagcgaatg
      961 cgggtccaca aacaccttca cgccacccga ctcgatcacg gtgtcctcgt cccgggactg
     1021 ctcttccaaa gacatgccgt actggaaacc atggcagccg ccgcccgaga catagatccg
     1081 taagccgccc gtggtctgct tctgctctgc ttccaagagg ctctgaactt tcttgccagc
     1141 ctgttccgtc acagtaatca ttgatgatca ccctccttat atataatatt atataggctc
     1201 ttactttaat tccctgacat cctgaattgc ttcctcatcc ggggtcctga caaccacgtt
     1261 gccatgaacc ttggcctgac aggccagccg atgcggggta acaagttctg ccacatccaa
     1321 catgttttct tcgtcaaact cgacctcgga aaggttctcc atgccgcttt cgatcaccac
     1381 acgacaagtg ctgcaattgg caaatccgcc gcagtcatgc ctgagttcat acccgaactg
     1441 ttccgcagcc tgcaagaggt tagttccgac tgcaacctca ccgcttttcc caatcgtttg
     1501 aaaggtgact ttcggcatac attttcttat caagatctta tcacggtacc gtatcctatg
     1561 tcaataagag attatctcaa gtagtggatc ttttgctaga atttcgataa aaagaatgtt
     1621 acgataccac aaaagcacag agggtataaa gacgatggat aagagagaac gggcctcttt
     1681 tcgccagcag ttgttgaaaa aactacgcga ttcctttgtt tacagtaatg agccgacctt
     1741 cacgctttcc tccggccgga aaagccattt ttacatcgac tgcaagaaag tcacactgga
     1801 tgcggaggga gccttcctcg ttggtcaatt gatcctcgat gcggtccgtg atttggaggt
     1861 ggacgcaatc ggcggcatga cgctgggagc ggatcccatc gcgactgcgg ccgcggtcct
     1921 gagctacgga ggggagcagg aaatcccggc ctttatcgtc cgtaaggaac ccaagcctca
     1981 tggcacctcc gcgtatattg aaggcaacgt cgcacgtgag tcccaggtgg ttgtggtgga
     2041 tgatgtcctg acaggtggac gatccaccga gcgaacaatc cggattttag aggaagccgg
     2101 ttgtaagata atcaaggtga tcgcccttgt ggatcgaaaa gaaggcggcc gcgagcgact
     2161 cgaagcgctc ggctacacgg tcgagagtct gtttacagtg gaagacctgc tgaagtccta
     2221 agctgtgccg gattccccta tccgggggca ttgtaatttc gagtgattac aggtataata
     2281 aaataaaaat tacctgagga aagacccatg gcctccaaaa agccggaaga agcaaaagga
     2341 cagaaaaaac ctgaagaagc caaaaaagaa aaaaagccca cccctttgac catcattcag
     2401 gttccgaacg acaaagagga actggaatca gaaatgcagg atctttttaa tgaagacaag
     2461 gtcgagcaca tccagggaaa tatcgagcct gaggcggatt agtccgcctt aagtcgttta
     2521 gccttttaag atctttgcca acctctctac atctttctct tccgtccaga cataagccgc
     2581 gtatttgccg cccagcgcaa tcgcgtccac tgcatgaagg ttgatcccct cctgggcgac
     2641 gcgatcgagt gtatcggaga gtgcgcccac gcggtcttct ccggtcagat gaaaacaggg
     2701 gccttccttc accgtccatc ctgaagcttg ggcagccttt ttgaatttgt ccgggtcttt
     2761 tggaattgcg atgatctccg cctttccctg acccttcgcg aacccccaga caccggccat
     2821 gtccacgctg gcttccatca tccgctttgc aaaacgagcc agctcgcctg gcttgtcctg
     2881 cgtgccgatc gagaaaaact tcactcgttt gatatccaca acaaacctcc cgaataaggg
     2941 ttggtattga taagagcggt aaaagcaggc ctgattgtag gtcgttttat cggagatgtc
     3001 aacgaccgag tttgatggtg ccctgctgga acccggagat taggccgagg aagaccagcg
     3061 caccgccgat gaccgaaaag ataaaaccga ttccaccaag cagaaaccac cgacgaacca
     3121 cccgacgctc cttctcatca agatgaacgt ggtactgctg aggcgacatc caccagcgcg
     3181 ggggaagctg acttcttggc cccaggattc ggtacaaaac gagatgataa taaaagccgg
     3241 tcggtacccc ttcgatcagc cccaacacca aaagccagag gccaatctcg gtgaacagaa
     3301 caggaggaat ctggttgctt aaagacccga gaaagaccag aaggacagcg ccta
//
LOCUS       DATMKH010000055         1329 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_669197,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000055 DATMKH010000000
VERSION     DATMKH010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669197"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..938)
                     /locus_tag="VNV63_00680"
     CDS             complement(54..938)
                     /locus_tag="VNV63_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HXC61161.1"
                     /translation="MKPKLAAFPKCYMDELCVHRTMTLNDWIEIGATLGVQGLEFYSG
                     FLEDNEAFLREAKASMEKHGLQMPMLCCSPDFTEPDPDLLEEQIARERRMIEITAFFG
                     GHLCRVLSGQRRPGLSIETGIAQVVRVIKSLLPFAEEHGVVLSMENHYKDSYWRYPEF
                     AQKTGIFTAIIEQIDSPWFGINYDPSNTILAGEDPLEMLDRVKNRVVSMHASDRYLNN
                     GTLEDLRREEDAVGYASRLSHGIIGHGLNDYDKIFSTLNSVGFSSWISIEDGMNGIED
                     MRESVRFLRAKIDLHFPG"
BASE COUNT          328 a          370 c          301 g          330 t
ORIGIN      
        1 ggctgtgatt tagctccctt cctcggtttt tctcttttct tcggtcatca atctcaaccc
       61 ggaaaatgca ggtcgatctt cgcacgaaga aatcgtacag attcccgcat atcttcgatg
      121 ccattcattc catcctcgat cgagatccag ctggaaaacc ctaccgagtt caaggtggag
      181 aaaattttgt catagtcatt gagcccatga cctataatcc cgtgcgagag ccgactggca
      241 taaccgacag cgtcctcctc tctccggaga tcctccaggg taccgttgtt cagatagcgg
      301 tcgctggcat gcattgagac cacccgattc ttgacccgat caagcatttc caagggatct
      361 tcaccggcga gaatcgtatt agaaggatca tagttaatgc caaaccatgg agaatcgatc
      421 tgttcgatga tcgccgtgaa aatacccgtc ttttgggcga attccggata acgccaatag
      481 ctatccttgt aatgattttc catcgacaaa acgacaccat gctcctccgc gaagggcaaa
      541 aggctcttaa tcactcggac gacttgggcg ataccagttt caatcgataa cccggggcgc
      601 cgctggccgg atagcacccg acacaaatgc ccgccaaaga aggccgtgat ttcgatcatc
      661 cgcctctcgc gagcaatctg ctcctccagt aaatcgggat cgggctcggt gaagtccgga
      721 gagcaacaaa gcatcggcat ctgaagtccg tgtttttcca tcgatgcttt ggcttcccgt
      781 aaaaaggctt cgttatcttc caggaatcct gaataaaact ccagtccctg cacaccaagc
      841 gtcgccccga tttctatcca gtcgttgagg gtcatcgtgc gatgcacaca caactcgtcc
      901 atgtaacact tagggaacgc tgccaacttt ggtttcatga tggtttgtcc ggcatcggtt
      961 acttaatggt ctgggttttg cttcgctcga atgtcaaaac gacggaaata atgatcatta
     1021 atccgaggat gctttattga aaataggggt cgatgccgac cactcctcaa ctcctcctcc
     1081 caacgttgaa cgccaaacgc tgccctgttg ccgttgacat ccggtcgcgt tgtggctaaa
     1141 caaatgattt gcctgctatc taatttgttg accgaaccgc ggcgcggcat caaaaaaagg
     1201 cttgcgccat gattgagatg aatattcaaa actctaagac cctccttgta ctcgcgaccg
     1261 tgctgagttt gacaccgttc gcgagcgctc agatcaaaag cttaaaggac gagtcatccc
     1321 caccgcccg
//
LOCUS       DATMKH010000056         2936 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3876624, whole genome shotgun sequence.
ACCESSION   DATMKH010000056 DATMKH010000000
VERSION     DATMKH010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3876624"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2456)
                     /gene="rpoB"
                     /locus_tag="VNV63_00685"
     CDS             complement(<1..2456)
                     /gene="rpoB"
                     /locus_tag="VNV63_00685"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HXC61162.1"
                     /translation="MTQFGMAGLKERKDFSKIQAQIDIPDLIEIQKKSYERFLQMIIR
                     PEQREDIGLQAAFNSVFPISDYNETAMIELMDYTLGIPKYDVRECLERGMTYAAPLKI
                     KVRLIHWEKEEKTTSKKVKDVKEQEVYLGELPLMTDNGTFIVNGTERVVVSQLHRSPG
                     VSFTHDKGRTHVSGKVLYSARIIPYRGSWLDFEFDARDILYVRIDRRRKMPVTILLKA
                     FGYTTEDLLRIYYPIEEIKVVKGKLFRKLDPQLHPGIMSPMDIVGKKSREVIAKEGNK
                     LTKALIEKIISAGVKEIPVTKEDLIGRPVLHDLIDSKTGEILLERNRDLTEEVLNEII
                     ERKIEQFNMIYIDNINVLPVIRDTLLTEKVTSKEEAMVEIYKRLRPGETPTIETARVL
                     FDSLFFSLKRYDLSPVGRLKLNKKLGLNFPLDKRTLTAEDILEVVRYLIDLKCGKGEI
                     DDIDHLGNRRVRSVGELLENQFRVGLVRMERTIKERMNLLDMENVLPHDLINAKPVTA
                     IIKEFFGSSQLSQFMDQTNPLAEITHKRRLSALGPGGLTRERAGFEVRDVHPSHYGRI
                     CPIETPEGPNIGLITSLATYARVNEFGFIEAPYRKVKNGRVTDEIAYLSAIDGDKYVI
                     AQANTKTDSHGKITADSVSARSSNEFVMVAPDKVEFMDVSPKQIVSVATAMVPFLEND
                     DANRALMGSNMQRQAVPLIQTETPLVGTGMEAVVARDSGYVVLAKRPGVVESVDASRV
                     VVRAGSGARKKKVRKGQRDLGVDVYSLIKFKRSNQNTCITQKPVVKVGTKVKQGQVLA
                     DGPAIDQGELALGRNVLVAF"
     gene            complement(2503..2904)
                     /gene="rplL"
                     /locus_tag="VNV63_00690"
     CDS             complement(2503..2904)
                     /gene="rplL"
                     /locus_tag="VNV63_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013163683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HXC61163.1"
                     /translation="MPKVKEEGALGKQEVITAIENMTVLELAELVKELEGKFGVTAAA
                     PVAVAAAAGGGAAAAAQEEKTTFDVILTNAGDKKIQVIKVVRELTNLGLKEAKDLVEG
                     APKPVKAGVTKEESETMKKKLEEVGGKVEIK"
BASE COUNT          667 a          836 c          657 g          776 t
ORIGIN      
        1 ataaacgcta ccagcacatt acgacccaaa gccagctctc cctgatcgat cgctggacca
       61 tctgccagta cctggccttg ctttaccttg gtaccaacct tgaccaccgg cttctgagta
      121 atacaggtgt tttggttaga acgtttaaat tttatcagag aataaacgtc cacaccaaga
      181 tccctttgac cttttcggac cttcttcttc ctggccccgg atcctgctcg taccacaact
      241 cgcgaggcat ccacgctttc aacaacaccg ggccgttttg ccaatacgac ataaccagaa
      301 tcccgtgcga ctacagcctc catcccggtg cccacgagcg gggtctcggt ctgaatgaga
      361 ggaaccgcct gccgctgcat gttggatccc atcagggcgc ggttggcgtc atcgttctcc
      421 aaaaacggaa ccattgcggt agcaacgctc acaatctgtt ttggagacac atccatgaat
      481 tccactttat cgggagcgac cataacgaac tcattggatg atctggccga aacgctgtcc
      541 gcggtaatct tgccgtgact atcggttttg gtattagcct gggcaatcac atatttatcc
      601 ccatcaatgg ccgagaggta agcgatctca tccgtgaccc gaccgttttt gaccttccga
      661 taaggggcct cgatgaaacc aaattcgttc acacgggcgt aggtagcgag tgaggtgatc
      721 agtccgatgt tgggcccttc cggggtctcg atggggcaaa ttcgaccgta gtgagaagga
      781 tgcacatccc ggacttcaaa tccagccctc tcccgagtga gccctccggg acccaaggcg
      841 gaaagacgcc gtttatgggt gatctcggcc agaggattgg tctggtccat aaattgggac
      901 agctggctgc ttccgaaaaa ctctttaatg atcgcggtca ccggcttggc gttgatcaga
      961 tcatggggaa gcacattttc catatccagc aggttcatcc gctccttgat ggtgcgctcc
     1021 atccgaacca gcccgacacg gaattgattt tcgagtaact cacctacgga acgcacccgg
     1081 cggtttccca ggtggtcaat gtcatcaatc tctccctttc cacacttaag atcaatgaga
     1141 taccgaacta cttccagaat atcttccgcc gtcaaagttc ttttgtccaa aggaaagttc
     1201 aaaccgagtt ttttgttgag cttgagacgt ccgaccggtg agaggtcata tcgcttaagc
     1261 gagaagaaaa gactatcgaa caaaacacgg gcggtctcga tagtgggtgt ctcacccggg
     1321 cggagccttt tatagatctc caccatggct tcctctttcg aggtcacttt ctcggtcagc
     1381 aacgtgtcac ggataaccgg caggacatta atattgtcaa tgtagatcat gttgaactgt
     1441 tcgatcttcc gctctatgat ctcgtttaag acctcttccg tgagatcccg attccgttcc
     1501 agcaggatct ctccggtttt agaatcaatg aggtcatgaa ggaccggccg gccgatcagg
     1561 tcttctttcg taaccggaat ctccttgaca ccagccgata tgatcttctc aatgagcgcc
     1621 ttggtgagtt tatttccctc tttagcaatc acctcccggc tcttcttgcc gacaatatcc
     1681 atcggtgaca tgatccccgg atgcaactgg ggatccaact ttctgaataa tttccccttg
     1741 accaccttga tctcttcaat gggatagtag atacgcagga gatcttccgt cgtgtaaccg
     1801 aaggccttga gaaggatcgt caccggcatc ttcctgcgac ggtcaatccg gacatagaga
     1861 atatcgcggg catcaaactc aaaatcaagc catgaacccc gataggggat aatacgtgcg
     1921 gaatagagga ccttcccgct tacgtgggtg cggcctttat cgtgggtaaa agaaacaccg
     1981 ggagaccgat gcaactgact gaccacgacc cgctcggttc cgttcacgat aaaggtgcca
     2041 ttatctgtca tcaggggcag ttcccccaga tagacttcct gttccttgac gtctttgacc
     2101 ttcttgctgg tggtcttctc ttccttctcc cagtgaatca accggacctt gatcttcagg
     2161 ggagccgcgt aagtcattcc ccgttcaaga cactcccgaa catcatactt gggtatacca
     2221 agggtgtaat ccatgagttc gatcatggcg gtctcattgt aatccgaaat cggaaagacg
     2281 ctgttaaaag ctgcctgaag accgatgtcc tcccgttgtt ccggtcggat gatcatttga
     2341 agaaatcgtt catacgattt cttctggatt tcgatcaaat ctggaatatc gatctgcgcc
     2401 tggatttttg aaaaatcctt tcgctccttc agtcctgcca tcccaaactg ggtcatagaa
     2461 atttttctcc ttggtacatt ttcaccgcac tttcaaaatc aactacttga tctcgacctt
     2521 gcccccgacc tcttccagtt tcttcttcat agtctcggat tcttccttag tcacaccagc
     2581 cttgactggc ttcggcgcgc cttccaccaa atccttcgcc tctttgaggc ccagattggt
     2641 aagctcacgc accaccttaa tcacctggat cttcttgtcg ccggcatttg tcaagatcac
     2701 atcaaaagtg gtcttctcct cttgagctgc agcagcagct ccaccgcctg cggcagcagc
     2761 aactgcgacc ggcgcagccg ccgtgacgcc aaactttcct tcaagctctt ttaccaactc
     2821 ggccagttca agaaccgtca tattttcgat cgctgtaatg acctcttgtt taccgagcgc
     2881 accttcttcc ttcaccttag gcatttccga ctcctttctt tctgactggg ttttct
//
LOCUS       DATMKH010000057         1043 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1951614, whole genome shotgun sequence.
ACCESSION   DATMKH010000057 DATMKH010000000
VERSION     DATMKH010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1951614"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..951)
                     /locus_tag="VNV63_00695"
     CDS             complement(<1..951)
                     /locus_tag="VNV63_00695"
                     /inference="COORDINATES: protein motif:HMM:NF024486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HXC61164.1"
                     /translation="MNWNSVSSQAEIIGLVSVVDAVENQSLNWSEIQQASEVSYDRWW
                     IDRVVTDDAVLCSFIAEQHEEMIRRFQELDAQVCKLSQAILKSKIDERLPDPKAFRTD
                     PEWGTLAREISKKARHLPLRQLFQRIPHALTQLAPCLMMSPLSISQYLAPNADLFDVV
                     IFDEASQIPVWEAVGAIARGRQTVIVGDPEQLPPTTFGERKLGEDDEYNDVVDQESIL
                     DECLAASLPQRSLNWHYRSQFESLISFSNQQYYKGQLVTFPSNVTEDKAVRYVHVPGG
                     VYERGKGRVNREEARVVVAEIVRRLTSGDFLLNKRSLGVVT"
BASE COUNT          238 a          308 c          246 g          251 t
ORIGIN      
        1 cgtgaccact cccagcgatc gcttattgag taggaaatcc ccactggtca aacggcgaac
       61 aatttccgcg accaccacgc gcgcttcctc ccggttgacc cgtccttttc ccctctcata
      121 gacacctcca ggaacatgaa cataccgaac ggctttgtct tcggttacgt tcgagggaaa
      181 cgtgaccaac tgacccttat aatattgctg attcgagaag ctaatcagac tttcaaactg
      241 acttctgtaa tgccagttca aagagcgttg cggcaaactt gccgccaagc attcatccag
      301 gatgctttcc tgatccacca catcgttata ttcgtcgtcc tcaccgagtt tccgttcccc
      361 gaaggtggtg ggcggcagtt gttcagggtc gccgacaatc accgtctggc gcccgcgggc
      421 aattgcccca acagcttccc agaccgggat ctgagatgct tcatcaaaga tcactacgtc
      481 gaaaagatcc gcattaggtg ccagatattg cgagatcgag agcgggctca tcatcagaca
      541 gggcgccagt tgagttaagg cgtgagggat cctttgaaag agttgacgca acggcagatg
      601 cctggccttc ttcgaaattt cccgcgccaa cgtcccccat tccggatcgg ttctgaacgc
      661 tttgggatcc ggcaatctct catcgatctt cgatttcaga atcgcttgcg acaatttgca
      721 gacctgggcg tctaattcct gaaatcgacg gatcatctcc tcgtgctgtt cagcgataaa
      781 cgagcagagc acggcatcgt ctgtcaccac tcggtcgatc caccaacggt cataggaaac
      841 ttctgaggcc tgttggattt ccgaccaatt gagcgattga ttctccacgg cgtccaccac
      901 ggaaacgagt ccgatgatct ccgcttgcga ggagacggaa ttccaattca tccaggtatt
      961 ggcgcgcccg atattcgctt tccatctgga aattaccgcc aggacttaaa tattccgcga
     1021 cctccgtgtc ctccttcgat gct
//
LOCUS       DATMKH010000058         1377 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1311067, whole genome shotgun sequence.
ACCESSION   DATMKH010000058 DATMKH010000000
VERSION     DATMKH010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1311067"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="VNV63_00700"
     CDS             <1..472
                     /locus_tag="VNV63_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXC61165.1"
                     /translation="FHSLSKTFNMTGWRIGFAVGNREILSGLGKVKSNLDSGAFQAIQ
                     EAGIEALLLDESVVEAIRRRYQERRDLLIPGLKRLGFKIEPPKASFYVWLRTPSGFTS
                     AKFAATLLQQTGILTTPGNGFGKSGEGYIRMTLTVAKERIEEVVRRIEKAGITG"
     gene            477..968
                     /gene="folK"
                     /locus_tag="VNV63_00705"
     CDS             477..968
                     /gene="folK"
                     /locus_tag="VNV63_00705"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008633520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HXC61166.1"
                     /translation="MAALICFIGIGSNLGNRQAFCLEALRRLEKLPMTRLAAVSSLYE
                     TEPVGAGYLSPFINGVAELSTELSPRAVLSAALSIEAGLGRDRSKVEPVRPMDLDLLL
                     FGDLILDEPDLTIPHPRLHERRFVLEPMAEIAPDVIHPVFKKKISDLLKRLSDPHQVR
                     RLL"
     gene            994..>1377
                     /locus_tag="VNV63_00710"
     CDS             994..>1377
                     /locus_tag="VNV63_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HXC61167.1"
                     /translation="MEDLRYIVIDGPIGVGKTSLAKLLSEELTARLILEKTEDNPFLK
                     EFYQNPKQFAFQTQVFFLLSRYRQLEALSQMDIFSNSTVSDYFFPKDRIFASVTLEES
                     ELALYKQIYNLLNPHIPIPDLVIYLQ"
BASE COUNT          317 a          382 c          359 g          319 t
ORIGIN      
        1 gtttcattct ctctcaaaaa cctttaacat gaccggctgg cggatcggct tcgcggtggg
       61 caaccgcgag atactgagcg gtctgggaaa ggtcaagagc aacctggact cgggtgcctt
      121 tcaggcaatt caagaggcgg ggatagaggc gctgctcctg gacgagtcgg ttgtagaggc
      181 gatccgtcgc cgctatcagg aacgacggga tctgctgatc cccgggctga aacggttagg
      241 gtttaagatc gagccgccca aggcctcgtt ctatgtctgg ctccgcacac cttcaggatt
      301 tacttccgca aaatttgctg ctacactcct gcaacagacc gggatcttga ccacacccgg
      361 caacggattc gggaagtccg gcgaaggtta tatccggatg accttgaccg ttgccaaaga
      421 acggatcgaa gaagtcgtcc gacggataga aaaggccggc atcactggct agaagcatgg
      481 ccgctctcat ctgttttatc ggaatcggat ccaatctggg aaatcgtcaa gctttctgtc
      541 ttgaggccct gcggagactg gagaaactcc cgatgacccg gctggcggca gtttcatcgc
      601 tctatgaaac cgaaccggtg ggagcagggt atctctcccc ttttatcaat ggtgtggccg
      661 aactttccac cgagctaagc cctcgggcgg tcttgtccgc cgcactttcg atcgaagcag
      721 gtctcggccg ggaccgctca aaggtcgaac cggtgcgtcc aatggacctc gacctattgc
      781 tctttggcga tcttatcctc gatgagccgg atctgacaat tccacatcca cggctgcacg
      841 aacggcgatt cgtcttggaa cctatggccg agattgcgcc agatgtcatc catccggtct
      901 tcaagaaaaa gatttcggat cttttaaaaa ggctgtccga tccgcaccag gtcagacgac
      961 ttctctagtc actcatctta atggaaggaa ggtatggagg atctgcgcta tatcgtaatc
     1021 gacggaccga tcggtgtagg gaagaccagc cttgccaaac tcctatcgga agagctgacg
     1081 gcccgcctaa tcttagaaaa aacggaagac aatcctttct taaaagagtt ctatcagaat
     1141 cccaagcagt ttgccttcca gacacaggtt ttctttttgc tgtctcgcta ccggcagctt
     1201 gaggcacttt ctcaaatgga catcttctcg aacagcacgg tcagcgacta cttctttccc
     1261 aaggatcgga tcttcgcctc cgtcacgctc gaggagagcg agcttgcgct gtataagcag
     1321 atctacaacc tgctcaatcc gcacatcccg atcccggacc tcgtgattta tcttcag
//
LOCUS       DATMKH010000059         1677 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_350175,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000059 DATMKH010000000
VERSION     DATMKH010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350175"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="VNV63_00715"
     CDS             <1..691
                     /locus_tag="VNV63_00715"
                     /inference="COORDINATES: protein motif:HMM:NF014592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HXC61168.1"
                     /translation="RIQTVLSPGRVHLVDLENDAVKIFSQRFGKTPLPPYIHRENGVY
                     EVQDRQRYQTVYAEKEGSIAAPTAGLHFTEGLLKQIQAKGVKIIPVTIHIGPGTFQSV
                     CTEIISDHRMEPEHFEVSMQSVQSILEAKRCGHRIVAIGTSTVRVLESLKLEDVTAAP
                     QQGSTCLFIFPGFQFKIVNALVTNFHLPRSTLLMLVAAFADRKPLLEVYQEALGQGYR
                     FASYGDAMLIL"
     gene            701..1318
                     /locus_tag="VNV63_00720"
     CDS             701..1318
                     /locus_tag="VNV63_00720"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HXC61169.1"
                     /translation="MRLIKRNQHQRHRDPSAWRPLLLGVVALLLVGIWVFLQMLDRPS
                     RSESGPQVVSEAKNPSSSTTSPVLSDRTPAETFTFYKTLKAPEHAKSEPQSLEPKPSV
                     RLPGRAAEPAPRPLDHVQKTRSKGYAVQVAAFRERQAAISLIHRLKKKGYPASLLVVE
                     TSGQETWYRVRLSPYTTHAQAEDVARRLKAEERLGSYIARLTEAR"
     gene            complement(1315..1596)
                     /locus_tag="VNV63_00725"
     CDS             complement(1315..1596)
                     /locus_tag="VNV63_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61170.1"
                     /translation="MAKSQIRGKSILAVAKDIAEGFFFVNTLILKKFESEDYKALHQQ
                     LKKLQNEVRSEKFPLHDTLAIRSRNVRLQRLHGALIILEHSARERNVVL"
BASE COUNT          382 a          406 c          461 g          428 t
ORIGIN      
        1 taggatccag acggtgttaa gtccggggag ggttcatctg gtggacctgg agaatgatgc
       61 ggttaaaata ttcagccaac gattcggcaa gactcctctg ccgccttata tccataggga
      121 gaacggagtt tatgaggttc aggaccggca acggtaccag acggtctacg ccgagaaaga
      181 aggctcgatc gcggccccga cggcgggact ccattttacg gaaggacttc tgaaacagat
      241 tcaggccaag ggtgtaaaga ttatccctgt gacgattcac atcggtcccg gcacgtttca
      301 atcggtttgt accgaaataa tttctgacca tcggatggaa ccggagcatt ttgaagtttc
      361 gatgcaatcc gttcaatcca ttttggaagc caagcgctgc ggccatcgga tcgtagcaat
      421 cgggacctct acagtgaggg tgttggagtc cttaaaattg gaggatgtta cggcggcgcc
      481 acagcaggga tcgacttgtc tctttatttt tccgggtttt cagtttaaga tagtcaatgc
      541 cctcgtgacc aacttccatc tgcctcggtc tacgctgctg atgcttgtgg cggcatttgc
      601 agatcgtaaa cctctgttgg aggtttatca ggaggctttg ggtcagggtt atcgttttgc
      661 aagttatggt gacgcgatgt tgattttgta ggacaaacga atgcgattga ttaagcggaa
      721 ccaacatcaa cggcacagag atcctagtgc ttggcgtccc ctcctgctgg gagtcgttgc
      781 cttacttttg gttgggatct gggtattcct ccagatgctg gatcggccgt cacgttctga
      841 atccggtccg caggtcgtat ccgaggcgaa gaacccctca tcgtccacca cctcacctgt
      901 tttatcggat cgcacccctg ctgagacatt cacattttac aagaccttga aggcccccga
      961 gcatgccaaa tcggaaccgc agagtcttga acccaaaccg tctgtccgtc ttccgggccg
     1021 tgctgccgag cctgctccca ggccattaga tcatgtccaa aaaacgcgtt cgaagggata
     1081 tgccgtccag gtggccgcct ttcgagagcg acaagcagcc attagtctga ttcatcggtt
     1141 gaagaagaag ggatacccgg cctccctgct ggtggttgag acgagcggcc aggaaacgtg
     1201 gtatcgggtc cggctgagtc cgtacactac acatgcccag gcggaggacg tggctcggcg
     1261 acttaaagcg gaggaacgac ttggttctta tattgcgcgt ttaacggagg cgcgctaaag
     1321 gacgacattt ctctcccgag cgctgtgctc caggatgatc aaggcgccgt gaagtcgttg
     1381 aagcctgacg tttcggctgc ggatggccaa tgtatcatgg agcgggaact tctcggatcg
     1441 aacctcgttt tgaagttttt ttaattgttg gtgaagagct ttataatcct ccgattcaaa
     1501 tttttttagt atgagcgtat tgacaaaaaa gaatccctcg gcgatatcct ttgcaacggc
     1561 gaggatgctt tttccacgga tctgggattt ggccatagtt ccaacttccc cgcttaaagc
     1621 ggagaacggt tcaagaagag tgcatgattt tctagtattt taagataccg gaatggt
//
LOCUS       DATMKH010000060         1148 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_992006,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000060 DATMKH010000000
VERSION     DATMKH010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_992006"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
BASE COUNT          276 a          294 c          302 g          276 t
ORIGIN      
        1 ttcgataagc ttgcggcatg caagaacatt tcttcctgcc aactacacca tctgtatttt
       61 tcagcgtttc aatgcccttc aacaaattcg tttttcacta agataaattt gccctttcga
      121 gggacctgca ggctgctaaa aatggattgt gaaagccaaa ggattgtttg gtgtgtcagg
      181 gaaggcttcg gcaggggacc gagagatcag ttggacacag gcgtacgaac ggcgaatgcg
      241 gagatacaat ctggaagtaa ttttggccgc attgatcgcc ctgatgattt tgaccacggg
      301 gttgtttctc gcgtggggag cctttcaaag cagcgcccgt cttccgcatc cgaggttgtc
      361 cgtccacagt aactaaaacg aggggtcccg aggaggatca tccttataag agacgccact
      421 aagggaggag gcttacaggt atcccgcccg cctgaggaag gatgcggcga gagccagcgt
      481 tctcttcttt ggactttgag agtccgagag ccagcgacca aaggtatgtc gattcacgcc
      541 aattcgagat gctatcgtcc gctcagtttc gtgagactcc tgtaagtacc gcctcaggat
      601 ctttatcagt tcatcagggc tcgtcgattt gttctcttcc gccgaattgg ctccgctcaa
      661 tttcctccgc ttctggtagg cgatcagaat ctatcggtat ctatcggctc aactttagga
      721 cgataatcag gcagaaacgg agtgaggacc ccggggaagc gccttcccgg agaccggaaa
      781 ttatgccgat tttctcggtt tgaatgtaag cgtcttccgg atttcgatga tcatcacgag
      841 gtcggatctt cccaaactcg catccaacaa tcgccacgca ggaactccag aagaaaacat
      901 ggccttctgg aggggagcat cgcctacttc ggtacgtact cggcgagaca gacgacactt
      961 gtgccatcca cattgagagc agtacgttcc cgaactggat cggaaccaat cagaaacgtt
     1021 cctttattat ccgaattgaa gtggactact ccgaccatgt cgctcggatc gggaaccgct
     1081 ctggatgtct ggaaacgagc cgagtagcaa gtatccggct agcaaactac agcgttggcg
     1141 gcagcgcc
//
LOCUS       DATMKH010000061         1505 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1633097, whole genome shotgun sequence.
ACCESSION   DATMKH010000061 DATMKH010000000
VERSION     DATMKH010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1633097"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="VNV63_00730"
     CDS             complement(<1..706)
                     /locus_tag="VNV63_00730"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HXC61171.1"
                     /translation="MVAIAPFRGFLYNPARIQDLRSVVAPPYDVINPEDQERYYQRHE
                     QNVIRLELGKELPGESSNENRYTRAMRYLQDWKKDRILLQDPESAYYFYVMDYKTPAG
                     VSRTVKGFFSLVRLGAYGQGGISPHEETFPKPKADRLALLRACRTNLSPIFGLYSDPS
                     SAILRQIEAARDPARPSIDLVEEDGIRHRLWKIQDPTAIHQVTGQMEKKRVLIADGHH
                     RYETALAFHQEESTEAS"
     gene            complement(852..>1505)
                     /locus_tag="VNV63_00735"
     CDS             complement(852..>1505)
                     /locus_tag="VNV63_00735"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HXC61172.1"
                     /translation="SILIGDYLYSKAVCQAVSLKNQEVNETLGEACRKMSEGEILQLS
                     HHENWQMTEEEYLKIVEGKTGSLMAATCRLGGILHQVPQEIKEALTFLGLHLGIAFQI
                     ADDTLDYTARQKRLGKSQGQDLKDGRITLPLLHLLSHCKESERKELERRVPSRGLGDD
                     DLSYILDRMEEFGSIAYAQSKARAYVGAAKKQLALFQDSPHRQSLSIMADYIVDRDH"
BASE COUNT          316 a          398 c          412 g          379 t
ORIGIN      
        1 ccgaggcctc ggtgctctcc tcctgatgaa atgccaaggc ggtttcataa cgatgatggc
       61 catccgcgat cagaaccctt tttttctcca tctgcccggt gacttgatgg atcgcagtcg
      121 gatcctggat cttccagagg cgatgacgga taccgtcttc ctccacaagg tcaatggagg
      181 gtcgagccgg atcgcgagcg gcttcgattt gtctcaagat ggcactggac gggtccgagt
      241 agagcccgaa aatcgggctc aggtttgtac ggcaggcccg aaggagggcg aggcgatccg
      301 ccttgggttt aggaaatgtc tcttcatgcg gtgagatacc gccttgaccg tatgccccca
      361 accgcacgag gctgaagaac cctttcactg ttctcgacac tccggcgggg gttttgtaat
      421 ccatcacata gaagtaatag gccgactcgg gatcctgaag aaggatacga tcttttttcc
      481 agtcttgcag ataccgcatc gctcgtgtat agcggttttc gttagaagat tcccccggaa
      541 gctctttgcc cagctcaagt cggatgacgt tctgctcatg tctttgatag tagcgctcct
      601 ggtcctcagg gttgatgacg tcataaggcg gggcaacgac actgcgcaag tcttgaatcc
      661 gggccgggtt atagagaaaa cctcggaaag gggcgattgc gaccattatc ggtatctctt
      721 gacggagaaa cgaaataggt attctcgaat ggccgcctgg gccaggagaa ccaggggatg
      781 aaaatccttt atcgccatat cggttgttgt ggtcgaagtc cgttgaatcg ttgtggtcga
      841 agtccgttga atcaatggtc ccggtccaca atatagtccg ccatgatgga caaactttga
      901 cggtgcggac tatcttgaaa caaggcaagc tgtttcttgg ctgcgccgac gtaagccctt
      961 gctttggatt gtgcgtaagc gatggacccg aactcctcca tccggtcgag tatataactc
     1021 aggtcatcat cgccgaggcc gcggctcggg acgcgtcgct ccagttcctt tcgttcgctc
     1081 tccttgcagt ggctcaggag gtgcaggaga gggagcgtga ttcgaccatc ttttagatcc
     1141 tggccttgtg atttccccag gcgcttttga cgggcggtgt agtccagcgt atcgtccgca
     1201 atctggaaag cgatccctaa atgaagtccc aaaaaggtca acgcttcttt gatctcctga
     1261 ggaacctggt gaaggattcc tcccagacgg caggtagcgg ccatcaggct tccggtcttc
     1321 ccttccacga tcttgaggta ctcttcctcc gtcatctgcc agttctcatg atgtgaaagc
     1381 tgcagaattt cgccttccga catcttgcgg caggcctcgc ccaaggtttc attcacctct
     1441 tgattcttca agcttactgc ttggcagacc gctttggagt agagatagtc tccgatcaaa
     1501 atgct
//
LOCUS       DATMKH010000062         1079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1312679, whole genome shotgun sequence.
ACCESSION   DATMKH010000062 DATMKH010000000
VERSION     DATMKH010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1312679"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..596
                     /locus_tag="VNV63_00740"
     CDS             168..596
                     /locus_tag="VNV63_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HXC61173.1"
                     /translation="MLHPENLGSKELATEKGTKAPEGATAGAGSGAVVGGMLGWLVGI
                     GALAIPGLAGAGVGGAMGGVTGALVGLGIPEYEARRYEGRLQKGGILVSVHCDTSAQI
                     KRAKEIMERTGAEDISSAGEASTDGKSSDTPSTKKVGMGY"
     gene            653..802
                     /locus_tag="VNV63_00745"
     CDS             653..802
                     /locus_tag="VNV63_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5670 family protein"
                     /protein_id="HXC61174.1"
                     /translation="MFIVLFAVLVLAWLGGFLVFHVSSALIHILLLFAVLSLIVHFFQ
                     RKTLS"
     gene            896..>1079
                     /locus_tag="VNV63_00750"
     CDS             896..>1079
                     /locus_tag="VNV63_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61175.1"
                     /translation="MMRSSTDLLQQRLLNRIAWLLWVIVLVLLMAFCFFASSFCITLL
                     LAAFLAILVDPLVTYLE"
BASE COUNT          243 a          259 c          308 g          269 t
ORIGIN      
        1 gttagagcaa tctaagcgcg tttgcaattc caattaagga atcaagctga ggacgaaaag
       61 aatatgtcaa aaaacaaatc agttctgggt atttactcga gtcggatcgg tgtggaaaac
      121 gcgatctccg ttttgaagga cccggctttg cgtatacgga cctgtccgtg cttcatccag
      181 aaaatctagg ttccaaagaa ttggcgacag agaaaggaac gaaagcaccc gaaggggcaa
      241 ctgccggcgc aggctcggga gcagtggttg gtggcatgct ggggtggttg gtcggcatcg
      301 gagcactcgc catcccggga ctggctgggg cgggagttgg tggcgccatg ggcggagtca
      361 ccggagcgct cgtaggcctc gggattcctg aatacgaagc caggagatat gaagggaggc
      421 ttcagaaggg tggaatcctg gtctccgtac attgcgatac ctccgctcag atcaaacgtg
      481 ccaaagaaat tatggaacgc actggcgccg aggatatctc ctctgcggga gaagcctcga
      541 cggacgggaa atcaagcgat acgccgtcaa caaaaaaggt aggaatgggt tattaagtca
      601 ggaactccag acccggaaga ttcagtccat cttggaaaag aaggaggctt acatgttcat
      661 cgtcctgttt gccgttttgg tgctggcttg gctgggggga ttcttggtct tccatgtatc
      721 cagcgctctg attcatattc tgttgctgtt cgcggttctc tcgctgattg tccatttttt
      781 ccagcgtaag accctatcgt gaactacttg tgcggtcctc cttgcgccca actggggatc
      841 gaggagacct cgcgcaagtg tttgcaaatg ataagaggga gagcaatcgg tctcaatgat
      901 gcgcagctca acggacttac tccaacagcg cctgctaaat cgtattgcct ggcttctttg
      961 ggtcatcgtg ctcgttcttc tcatggcttt ttgtttcttt gcaagttcct tctgcatcac
     1021 gctgctgctc gctgcctttc tcgcgatcct cgttgatcca ctggtcacct atctggaga
//
LOCUS       DATMKH010000063         1017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4200137, whole genome shotgun sequence.
ACCESSION   DATMKH010000063 DATMKH010000000
VERSION     DATMKH010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4200137"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..111)
                     /locus_tag="VNV63_00755"
     CDS             complement(<1..111)
                     /locus_tag="VNV63_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61176.1"
                     /translation="MEFTVSKTDLVRELSLSQGVVEKKTTIPILSNVLVEA"
     gene            complement(397..>1017)
                     /locus_tag="VNV63_00760"
     CDS             complement(397..>1017)
                     /locus_tag="VNV63_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HXC61177.1"
                     /translation="FNELYDHQKQIVISSDSAPKSTPGLVERLRSRFEWGLMVDIQPP
                     DLETKMAILDKKAEMEGVTLPEDVRIYIATKTKSNVRELEGALVKMIAYSSVTGTPIT
                     LAMAQQILKYLIPGQERRVTMESVLRAVAERFQMQPAQLKQKTNERKIAYPRQIAMYL
                     AKELTPASLPEIGRAFNGKHHTTVLHSIQKIDQLRHQDSDLNSLIH"
BASE COUNT          217 a          281 c          248 g          271 t
ORIGIN      
        1 cgcctcaacc aggacattcg acaagatcgg aatcgtcgtc ttcttttcca ccacgccctg
       61 ggacagactc aactcccgaa ccagatccgt cttgctaaca gtgaactcca tcgtctcccc
      121 tcgccttttg tccttgcttc ccttgagtcc ggctagctct aatccctctt cataaattgc
      181 tatgtactag aaccgtatta gtaggagctg ttgaaaagtt gatttctctc taaattatac
      241 aaaaatcaga agcttcttgc cactcaaacc tgtgaatctt tgggcgccgg gtcgtctaaa
      301 aattcgtgtc tcccggttcc atccacggta ctcacagggc gtccagtcta tcttctgtgg
      361 aaaaacggta taaccctaat ggattgaatc aactagctag tggatgaggc tgttcaaatc
      421 cgagtcctgg tgccggagtt ggtcaatctt ctggatcgag tgtaagaccg tggtgtgatg
      481 tttgccattg aacgcacgtc caatctccgg cagtgacgct ggtgtcagct ccttggccag
      541 gtacatagcg atctgccggg ggtaggcaat cttgcgctca ttggtcttct gcttgagctg
      601 ggcgggctgc atttggaacc gctccgccac cgctcgtaag accgattcca tcgtgacgcg
      661 ccgttcctga cccgggatca ggtactttaa aatctgctgg gccatggcga gtgtaatggg
      721 agtacctgtt acggatgaat acgcgatcat cttcaccagg gcgccttcca gctcacgaac
      781 gttagatttc gttttcgttg cgatatagat gcggacatcc tccggaagcg tgacaccctc
      841 catctccgct ttcttgtcga gaatcgccat tttggtctcg agatccggcg gctggatgtc
      901 caccatcaac ccccactcaa accgggagcg taagcgttcc accagaccgg gtgtgctctt
      961 gggtgcggaa tcgcttgaaa tcacgatctg cttctgatga tcgtagagct cattgaa
//
LOCUS       DATMKH010000064         1214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_31883,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000064 DATMKH010000000
VERSION     DATMKH010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31883"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            282..1082
                     /locus_tag="VNV63_00765"
     CDS             282..1082
                     /locus_tag="VNV63_00765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HXC61178.1"
                     /translation="MPRAIGSLVTDRFIVANKSFLRVIGLGCDEISSVALSEIVTIDS
                     NSYEKSTSGRLLTIKVRAFGEDLTMAGQAALVDQNLVVHENLAPEFMTAVFSLDLIRT
                     RLEKENHPCAAELKAIEERITNPLQRMRDEIQKSDADQREYYLMSRRLAAIVENSDDA
                     IISKDLNGMITSWNQGAERIFGYAADEMIGSSIRRIIPQDCQREEDEILAHLMRGERR
                     DHFQTVRMTKEGRHVYVSLTISPIKDETGQVIGASKVARAITDRDRPR"
BASE COUNT          319 a          302 c          320 g          273 t
ORIGIN      
        1 ccggctttaa ccctaggcaa cccgagcacc tttcatggct gtgtccttcg ggtgacacca
       61 ttcgctgctc tcctttactt gtcggaagaa gcacaccgat tgacctgtga tcccccgcat
      121 gcagatcgac gatcagtaag caatgtttcc tggcgattcc atagcggccg ggaaatcggc
      181 aattggaatt tacaaagaac gcactatgat aggatctttc tgcgtcatat ccgatgtacg
      241 agtcgatcaa atcggcgtgc aaggtcctgg ataggttcgg cttgcctcgt gcaataggga
      301 gtctcgtaac cgatcgcttc attgtcgcaa acaagtcctt cctgcgggtc atcggtctag
      361 gctgcgacga aatctccagc gttgctctat cagaaattgt gacaatagat tcaaattcct
      421 acgagaagag tacatccggc cgattactca cgatcaaagt gcgtgctttc ggcgaagatc
      481 tcaccatggc tgggcaggca gctcttgttg atcagaattt ggtcgtccat gagaatctcg
      541 cgcccgagtt tatgaccgcc gtcttttcac tagacttgat ccgaactcga ttggagaagg
      601 aaaaccatcc gtgcgcagcg gaattgaaag ccattgagga gcggatcact aatccccttc
      661 aacggatgag agacgagatt caaaaaagcg atgcggatca gcgggaatac tatctgatgt
      721 cccgccgctt agcagcgatc gttgaaaact cggacgatgc gatcatcagc aaagatctga
      781 acggaatgat caccagctgg aaccagggag cggaacgaat cttcggctac gcggccgatg
      841 aaatgatcgg cagctcgatc aggagaatta tcccccaaga ttgccagaga gaggaagacg
      901 agatcctggc gcatcttatg cgaggcgaac gccgagatca tttccaaacg gttcgaatga
      961 ctaaagaggg gcgccacgtc tacgtttcgc tgacaatttc tccgataaaa gacgaaacgg
     1021 gccaagtgat cggcgcctca aaagttgccc gcgcgattac ggatcgagat cggccgagat
     1081 agcattggct gctttagcaa gcagtgattt caacattctt cgggtggtca gtggtagaac
     1141 accaattata acagggtttt gataggtacg gtacaaatgg aggaccctcg gaccggaaga
     1201 ataacccttc tggg
//
LOCUS       DATMKH010000065         1483 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1314972, whole genome shotgun sequence.
ACCESSION   DATMKH010000065 DATMKH010000000
VERSION     DATMKH010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1314972"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            12..833
                     /gene="kdsA"
                     /locus_tag="VNV63_00770"
     CDS             12..833
                     /gene="kdsA"
                     /locus_tag="VNV63_00770"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017951875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HXC61179.1"
                     /translation="MRTVEVRGIPIGSEHPAVLIAGPCVIENKRLILDTAKALQEIAV
                     SVDIPFIFKSSYDKANRSSVRSFRGPGITEGLKILEMVKKELDLPLLSDVHTPEEAKF
                     AASVLDILQIPAFLCRQTDLILAAAMTGKVVNVKKGQFLAPWDMKNIVEKIESTGNHQ
                     ILLTERGTSFGYNNLVTDFRSLPILRGLGYPVIFDATHSVQLPGGAGNASSGQREFVP
                     HLARAAMAVGCDGLFMEVHPKPDQALSDGPNMVPLSELKSLLLQVKQIERALRGE"
     gene            835..>1483
                     /locus_tag="VNV63_00775"
     CDS             835..>1483
                     /locus_tag="VNV63_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015837877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HXC61180.1"
                     /translation="MSIEQGKRVLRIEAQAILDLIQRLDERFTQAVELMYRCTGKVVV
                     TGMGKSGLIGKKITATLASTGTPALFLHSAEGIHGDLGMLSRGDVVIAISNSGETEEI
                     IKLLPAIKRLNVKLISLIGTTKSTLAKNSDVVMDVSVKEEACSLGLAPTASTTATLAM
                     GDALAIALLEKRGFKEEDFAAIHPGGILGRRLLLKVEDVMHSGKEIPIVLEETPMK"
BASE COUNT          355 a          365 c          416 g          347 t
ORIGIN      
        1 gaaggcgtgc gatgagaacg gtggaggttc ggggaattcc gatcggctct gaacatccgg
       61 ctgtgctgat tgcaggcccc tgcgtgattg aaaacaagcg gcttatttta gacaccgcca
      121 aggcactgca agagattgct gtttcagttg acattccatt catttttaaa tcttcctacg
      181 ataaggccaa ccggagttcg gttcgatctt tccgcggtcc gggtattacg gaaggcctga
      241 agattctgga gatggtgaag aaggagctgg acttgccgct gctttcggat gttcatactc
      301 cggaagaggc aaagttcgcc gcctcagtct tagacatcct ccagatcccg gcctttcttt
      361 gccgtcagac cgatctcatt ttggctgcgg ccatgaccgg taaagtggtc aatgtgaaga
      421 aagggcagtt tctggccccc tgggacatga agaacatcgt cgagaaaatt gaatccaccg
      481 gcaatcatca gatccttctg accgagcgcg gaacgagctt tggatacaat aacctggtca
      541 ctgatttccg atcacttccg attctcaggg gtctaggcta cccggtgatc tttgatgcaa
      601 cccacagcgt acagcttcct ggaggtgccg gaaacgcttc ctccggacaa cgcgagttcg
      661 tgcctcacct ggcccgcgct gccatggcgg tcggctgcga tgggcttttc atggaagtcc
      721 atccaaaacc ggatcaggca ctctcggatg ggccgaacat ggtgccgctc tctgagctta
      781 aatccctttt gttgcaggtc aagcagatcg agcgggcttt acgaggagag tagcatgagc
      841 attgagcaag gaaagcgggt tcttcgtatc gaggctcagg caattctgga tctcatacaa
      901 cgtctggatg agcggtttac acaggcagtg gaactgatgt atcgctgcac gggcaaagtt
      961 gtagtgaccg gaatgggaaa gtcggggctc atcggcaaga aaataaccgc aacgcttgcc
     1021 agcaccggca cgccggcgct ctttctgcat tctgccgaag ggatacacgg cgacctgggc
     1081 atgctttctc gaggagatgt ggtaatcgcg atctccaata gtggagaaac cgaggagatc
     1141 atcaagttac ttcccgccat caaacgactc aatgttaaac tgatcagtct gatcgggacc
     1201 acaaaatcta ctttggccaa gaacagcgat gttgtgatgg atgttagtgt gaaagaagag
     1261 gcctgttctc tgggactggc gcctacggcc agcaccaccg cgacgttggc gatgggggat
     1321 gcactggcca tcgccttatt ggaaaaacgt ggttttaaag aggaagattt tgcggcaatt
     1381 catccaggag gtatacttgg acgtcgcctc cttcttaaag ttgaggatgt tatgcattcc
     1441 ggaaaggaga ttcccatcgt attggaagaa acccccatga aag
//
LOCUS       DATMKH010000066         1108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3559695, whole genome shotgun sequence.
ACCESSION   DATMKH010000066 DATMKH010000000
VERSION     DATMKH010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3559695"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1006
                     /locus_tag="VNV63_00780"
     CDS             <1..1006
                     /locus_tag="VNV63_00780"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HXC61181.1"
                     /translation="QGRVVISLDSLGLSPKDLKQIQSFIRRRKGIILLTGPTGSGKTT
                     TLYGMINAIKSKEVNIVTVEDPIEYNLEGINQIQVNPEIGLTFAHCLRALLRQDPNVI
                     LIGEIRDLETAEIAFRAAMTGHLVLSTLHTNDAAGSVTRLTDIGIPRYLVASLLIGVI
                     AQRLVRTICPKCKELRDPPVESMLGFQLPLEAMATAKLYYGKGCSYCNFIGYYGRTAL
                     FEILELTPRMREMIITGSSEQDLRSAALTAKMSSLGEDGLNKVKDGLTTIEELMRVIE
                     MQEGLETLCTTCGHYLHLDFLVCPHCGTLATNHCAGCGKPLQPEWKVCPYCRQAVQAV
                     "
BASE COUNT          246 a          286 c          309 g          267 t
ORIGIN      
        1 tcaagggcgt gtggtgatct cgctggacag cttggggctc tcgccgaagg atctcaagca
       61 gatccagtct ttcatccggc gacgaaaggg gatcattctc ttgaccggtc cgaccggcag
      121 cggaaaaacg acgaccttgt acggcatgat caatgcgatc aaatcgaaag aggttaatat
      181 cgtgaccgtg gaagacccca ttgaatacaa cctggaagga atcaatcaga tccaggtcaa
      241 tcccgaaatt gggctcacct tcgcccactg cctgagggcg ctcctgcggc aggatcccaa
      301 tgtgatattg attggggaga tccgggattt agagacggcc gagatcgctt tccgggctgc
      361 gatgaccggc catcttgttc tctccacact tcataccaac gatgcggctg gcagcgtcac
      421 gagactgact gatatcggaa taccccgtta tctcgtggcc tctctcctca tcggtgtcat
      481 tgcccagcgt cttgtgcgta cgatctgtcc caaatgcaag gaactgaggg acccgcccgt
      541 ggaaagcatg ctcgggtttc agctgccctt agaggcaatg gcaacagcca agctttatta
      601 cggtaagggc tgctcctact gtaattttat tggatattac ggccggacgg ctctcttcga
      661 gatcttggaa ttgacccccc gtatgaggga gatgattatt accggctcct cggagcaaga
      721 ccttcggtct gcggcgctca cggccaagat gagttcgctg ggcgaggatg ggctcaataa
      781 ggtgaaagat ggattgacca cgattgaaga actcatgcgg gtgattgaga tgcaagaggg
      841 tctcgagaca ctttgtacca cctgtggaca ttatcttcat ctggatttct tggtctgccc
      901 acactgcggt accttggcta caaaccactg cgcgggctgc gggaaaccgc ttcagccgga
      961 gtggaaggtt tgtccttatt gtcgtcaagc ggtccaggcg gtttaagaac gttttgattt
     1021 cttcgacccg atgtttatcc gtagattgtc aatgagccgg gtcttcccga tccaggccgc
     1081 gattgcgatc agtgtagaat ctgcgatc
//
LOCUS       DATMKH010000067         1235 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_355862,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000067 DATMKH010000000
VERSION     DATMKH010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355862"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            140..322
                     /locus_tag="VNV63_00785"
     CDS             140..322
                     /locus_tag="VNV63_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61182.1"
                     /translation="MSTIRVVLADDYRPIVAKVRGILGEEFEVIGTAENGNEAVDLVL
                     TLDADVLITDIFAYRL"
     gene            319..570
                     /locus_tag="VNV63_00790"
     CDS             319..570
                     /locus_tag="VNV63_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61183.1"
                     /translation="MNGLQAAERLQMANGRIVFLTIYQEEAYVRAAFDTGASAYVLKS
                     RLLTDLIPAIREALLGNIFVSKPLKFASLDGSSNKAFVS"
     gene            919..>1235
                     /locus_tag="VNV63_00795"
     CDS             919..>1235
                     /locus_tag="VNV63_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61184.1"
                     /translation="METLPRLQEAEIKSEVQRIIAELDVALSFLREADETSDKQFQRR
                     LIRYAQIAHLAVLRLQPSLALTGPEGIRINVRLLQIRANLKRYGISVARTLQEEMLIN
                     QWK"
BASE COUNT          299 a          323 c          326 g          287 t
ORIGIN      
        1 aggaagaccg tgttggcgtc cgcggccaga gttcactgtg gagtacaggc gacattttgc
       61 gccagcgagc aatactggat tcccaaaacc ccgcaacagg tgaacgataa cggtgttagc
      121 gccgttagtg cggaggcgta tgtccacgat tcgagtggtg cttgcagatg attatcggcc
      181 catagtcgcg aaggttcgcg gaatacttgg cgaggaattc gaggtcatcg gcacagcgga
      241 gaatggtaac gaagccgtcg acttggttct gaccctggat gccgacgtac tcatcacaga
      301 tatatttgcc taccgattgt gaatggactt caagctgccg aacggctcca gatggctaat
      361 ggtcggatag tatttctcac catctatcag gaagaggcct atgttagagc agcattcgat
      421 accggggctt ccgcctacgt cctcaagtcc cggctcttga ccgatctgat tccagcaatc
      481 cgcgaggctc tcctggggaa catatttgtg tctaagcccc tgaaatttgc ctctcttgat
      541 ggttcttcca acaaggcgtt tgtttcctga aagggaaaag aggagtggaa tgagtcatct
      601 ctggcaccct cctttcttaa gacaacacaa cattgttcgc cgattggaaa tattccaccc
      661 agccgtcttc aggatgaagg aaacgtcact ttttcccgac aaccttcccc acgacctatc
      721 acatcgaaat taatatccgt tgtggagaga tgctcggcgg ccgaagcgca tgggccgccc
      781 gcacctggga gggcactccg ccctaacggg ggccctcctt tctcttggct agtacgggat
      841 gtcgggagtg atcctggcct cagcgaatcc gcggatcgca cctccaaaat aacccatttg
      901 gcgcgaggtc caactgcgat ggaaacctta ccgagattgc aagaggcgga gatcaaaagt
      961 gaagtgcaga ggatcatcgc cgaacttgat gtagccttgt cttttcttcg cgaagcggat
     1021 gaaacctccg ataagcaatt tcagcggcga ttaatcaggt atgcgcagat cgcgcatcta
     1081 gcggttcttc gattacagcc tagtctggct cttaccggtc ctgagggaat acgaatcaac
     1141 gttcgactat tacagattcg tgccaatttg aagcgctacg gcatctctgt cgccaggaca
     1201 ctgcaagaag agatgctcat caaccaatgg aaaag
//
LOCUS       DATMKH010000068         1018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3241556, whole genome shotgun sequence.
ACCESSION   DATMKH010000068 DATMKH010000000
VERSION     DATMKH010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3241556"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="VNV63_00800"
     CDS             <1..338
                     /locus_tag="VNV63_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61185.1"
                     /translation="TGDAVGHWEKDTLVIDVIGLDERSHGMRGAAFFHSDQEHVIEHI
                     SRPSMNYLVYQVTIEDPKVLTKPWNSAPRRWSLSRYHESLEEFYCTNEQDTKEFRAVG
                     ETAEGKKSK"
     gene            complement(446..>1018)
                     /locus_tag="VNV63_00805"
     CDS             complement(446..>1018)
                     /locus_tag="VNV63_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61186.1"
                     /translation="KSFDQNGVPTNVPEGHYPGEYCEPQGIPVTFNFANEYPMLFMML
                     PDRIYQMFEDHREWRTIWLNRDHPKDLLASYFGDSIGKWEGNTLVVDTIGYNGEKGHR
                     WISENVGHYMSDAFHLVERYHLVDATHLELEMTYYDPKAWGDKPWTGFKKSFKLDSKS
                     DSLQENVCDPARVYDDMIEHPAGLPPRPTE"
BASE COUNT          262 a          256 c          262 g          238 t
ORIGIN      
        1 acacggggga tgctgtcggg cattgggaaa aagatacgct tgtcattgat gtgatcggct
       61 tagatgagcg gagccacggg atgcgaggtg cggcgttttt ccacagtgat caggagcacg
      121 tgatcgagca tataagccgg ccttcgatga attatctcgt ataccaagta accatcgagg
      181 atccgaaagt gctaaccaaa ccctggaact cggcgcctcg cagatggtcg ctctctcggt
      241 atcacgagag cctggaagag ttctattgta caaacgagca agacacaaaa gagtttcggg
      301 ccgtgggcga aacagccgaa gggaaaaagt caaagtaacc tcaaagcccg tcttcaagtc
      361 gagattggcg ctgcatacga gttttccgca ccggggtccg caggcttagt ccattacaca
      421 tagcccttaa gcaacccaaa aagacttact cggtgggtct aggcggaagc ccagcgggat
      481 gctcaatcat gtcgtcgtag actcgtgcag gatcgcagac attctcctga agagagtcgc
      541 ttttcgaatc gagcttgaag gatttcttaa atccagtcca gggtttgtcg ccccaggcct
      601 tcggatcata ataggtcatc tcgagttcaa gatgagtagc atcaacgaga tggtaacgct
      661 caaccaggtg gaaagcgtcg ctcatatagt gtccgacgtt ctcactaatc cagcggtggc
      721 ccttttcacc gttatatcca atggtatcga ccaccagcgt atttccttcc cacttgccaa
      781 tagaatcccc gaaatagctg gccagcaagt ctttgggatg atcccggttc agccagattg
      841 tccgccattc acggtggtcc tcgaacatct ggtagatccg atctggcaac atcatgaata
      901 acatcggata ttcattggca aagttgaaag ttaccggaat gccctgcggc tcacaatatt
      961 cacctggata gtgcccctcg ggaacattcg tggggactcc attttgatcg aatgattt
//
LOCUS       DATMKH010000069         1365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4523798, whole genome shotgun sequence.
ACCESSION   DATMKH010000069 DATMKH010000000
VERSION     DATMKH010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4523798"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1248)
                     /gene="zwf"
                     /locus_tag="VNV63_00810"
     CDS             complement(<1..1248)
                     /gene="zwf"
                     /locus_tag="VNV63_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019942609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HXC61187.1"
                     /translation="MANHKKPPASLIFIFGGSGDLNYRKLSPALYNLFIDDWMPEKFG
                     IVGIGRSPYNDEDYRIRLLNGIKQFSRRKSEADGHWAEFSKHVSYLQMDAEKDGDYGK
                     IADLVKAKEAEIGEHPNVIFYLSVAPQLMPDIAKKLGALNISSESATTRLVFEKPFGH
                     DLQSAHDLNQLLMGMFKEEQIYRIDHYLGKESVQNILSLRFANALFEPIWNRNYIEHV
                     QITAAETVGVEGRGGYYEQSGALRDMVQNHILQLMCMIAMEAPVNFDANEIRNKKVDV
                     LNAIRKINKEDVNAFAVRGQYGEGWMKGEKVPGYKQEKSVNPASAVETFAAVKFYIDN
                     WRWQDVPFYVRTGKRLNQKASNITVQFKKAPSYAFPPEAAETWKANRLTISIQPEMEV
                     RLRFQAKRAGQSMTLAPVEMIYNP"
     gene            complement(1258..>1365)
                     /locus_tag="VNV63_00815"
     CDS             complement(1258..>1365)
                     /locus_tag="VNV63_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61188.1"
                     /translation="ERMPTNLIQAQRDYFGAHTYQRIDEPGSFHTEWNK"
BASE COUNT          272 a          341 c          389 g          363 t
ORIGIN      
        1 tgggttatag atcatctcga ccggggcaag ggtcatggac tgaccagccc ttttggcctg
       61 gaaccgaagc cggacctcca tctcgggctg gatgctgatc gtgaggcggt tggctttcca
      121 ggtctctgcc gcttccggcg ggaaggcata gctgggggcc tttttgaatt ggacggtgat
      181 gttggaagcc ttctggttca ggcgtttccc ggtgcgaaca tagaagggaa cgtcctgcca
      241 gcgccagtta tcgatataga atttgactgc tgcaaaagtc tccacagctg aagcggggtt
      301 gacgcttttt tcctgtttgt agcccggtac tttttcgcct ttcatccagc cttccccata
      361 ctggccgcga accgcgaatg cgttgacgtc ctccttgttg atcttgcgga tcgcgttgag
      421 cacatcgacc ttcttgttgc ggatctcgtt agcgtcgaag ttgacgggcg cttccatggc
      481 gatcatgcac atcagctgga gaatgtggtt ctgaaccatg tcacgaagtg ctccggattg
      541 ctcgtaatag ccgccgcgac cctccacacc aacggtctct gcagcggtga tctggacgtg
      601 ttcgatataa ttgcggttcc agatcggctc gaacaaggca ttggcaaaac ggagcgaaag
      661 gatattctgt accgactcct tacccaggta gtgatcgatg cgatagatct gttcttcctt
      721 gaacatgccc atcaacagct gattcaggtc atgggcgctc tggaggtcat ggccgaaagg
      781 cttctcaaag acgagtcggg tggtggccga ttcggaggag atattcagtg cgccgagttt
      841 ctttgcgata tccggcatca actggggtgc gacggagaga tagaagatca cgttcgggtg
      901 ttcgccgatc tcggcctctt tggccttgac aaggtccgcg atctttccat aatcaccgtc
      961 cttctccgcg tccatctgga ggtagctgac gtgtttggag aattctgccc aatggccgtc
     1021 ggcctcactc ttcctgcggc tgaattgttt gatcccgttg aggaggcgga tgcgatagtc
     1081 ttcgtcgtta taagggctgc gaccgatccc gacgattccg aatttctcgg gcatccagtc
     1141 gtcgataaat aaattatata gcgcggggga aagtttcctg taattcaggt caccgctgcc
     1201 cccaaaaata aagatgaggg aggcaggtgg ttttttatgg tttgccataa tcgattctta
     1261 tttattccat tctgtatgaa aactgcctgg ctcgtctata cgctgatagg tgtgggcgcc
     1321 gaaatagtcg cgctgtgcct ggataaggtt ggtaggcatg cgttc
//
LOCUS       DATMKH010000070         1408 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1637076, whole genome shotgun sequence.
ACCESSION   DATMKH010000070 DATMKH010000000
VERSION     DATMKH010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1637076"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(306..620)
                     /locus_tag="VNV63_00820"
     CDS             complement(306..620)
                     /locus_tag="VNV63_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61189.1"
                     /translation="MASLRKGIPELLLDTDRFVETANAAIQLKNSAGFLSMPKKKPQI
                     SGVFGPVEMCELSELSELSPQDAVGSLSAKGRNGGIGLFFEETQGLGHTILSGGSLFN
                     HL"
     gene            complement(703..1128)
                     /locus_tag="VNV63_00825"
     CDS             complement(703..1128)
                     /locus_tag="VNV63_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013952025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXC61190.1"
                     /translation="MGEIGAEVLFIAGFGPIDRDVAESRALYSDSLGIPFKEDAGGYL
                     YTGSLEGAKHFALWPLSQAAQSCFGTDSWPDNFPVPQAWLEFEVENVENATADLESRG
                     YRMLMKNKTEPWGQTVSRFLSPEGLLIGVTFTPSMREKK"
BASE COUNT          342 a          424 c          330 g          312 t
ORIGIN      
        1 agcttcagtc cggacccatc ccctggagcc accggccctg aggcacgctt ctctttccat
       61 ccctcagggc ttatcgcgct tatagccttt ctagcccacc tgaaatttgg caaaccatcc
      121 cctgggacgc cgtctccgta caatgagcgc gcatacgcgc ctttgccacc acccaggcaa
      181 gatgatcaat aattaatttg atcatcttga tcatcttgat catcttcgtg aaacaaagga
      241 cttatttggc acgaccccac cgctgagccg ccaaacccca aaaaaagacc caaaaatatc
      301 aaattttaca aatggttgaa caggctgccc ccggacaaga tagtgtgacc taatccctgc
      361 gtctcttcaa aaaagagacc tatcccacca ttacgcccct ttgcggagag agaccccacc
      421 gcatcctggg gacttagttc gcttagttcg cttagttcgc acatttctac aggaccaaag
      481 acgccggaaa tctgcggttt cttcttgggc atagaaagga agccggccga attcttcaat
      541 tggatagctg cattcgcggt ttccacaaac cgatcagtat ctaaaagtaa ttcgggtatg
      601 cctttccgca gagacgccat cgactcgact gagacaagca cagcattgct cccgggctgc
      661 gtactctttt cgacgtcaag atattccctc cgtcgtgagg gactatttct tctcccgcat
      721 cgagggagtg aaggtgacac cgatcagcag accctcaggt gagaggaacc tgcttacggt
      781 ctggccccaa ggttcagtct tgtttttcat gagcattcga tatcctcgcg actcgagatc
      841 ggccgtggca ttctccacgt tctccacctc gaactccagc caagcttgcg ggacgggaaa
      901 gttgtcgggc caggaatcgg tgccgaagca ggattgcgcc gcctgcgaga gaggccacag
      961 ggcaaagtgc ttcgcacctt caagcgagcc cgtatacagg tagccgccag catcctcttt
     1021 gaagggaatg cccaggctgt cgctgtacag cgcacgactt tcagcaacgt cgcgatcgat
     1081 cggaccgaac cccgcaatga aaagaacttc tgcgcctatc tctcccatgt tcgccgcgcc
     1141 ctccttttca ggataccgat cattgcttcg cacacctccg cggcattagc tgtgattgga
     1201 taaattcctc ggagaatcgt cgcaatctcc aaggagtttt atccgacttc ggcctaccgg
     1261 taaaagcggg acggagaaaa gcgagacgaa cggaaaagac cctaaccgaa aaggctccgc
     1321 cgtcttgata acagacacga tttcacttgg ggccatttcc gtcccggatt agccccagca
     1381 tctagaagcg accgccgtcg ttaacaag
//
LOCUS       DATMKH010000071         1909 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2922419, whole genome shotgun sequence.
ACCESSION   DATMKH010000071 DATMKH010000000
VERSION     DATMKH010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2922419"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..296)
                     /locus_tag="VNV63_00830"
     CDS             complement(54..296)
                     /locus_tag="VNV63_00830"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXC61191.1"
                     /translation="MSSKSSRAGKTLIQWKLVGRRIRELRGFDITQEEFAQRIGISQG
                     HLSSVERGEKEMGAEILLRISQAFGKSIEWLLTGEG"
     gene            438..1214
                     /locus_tag="VNV63_00835"
     CDS             438..1214
                     /locus_tag="VNV63_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61192.1"
                     /translation="MSKIVVNKQSNRIRSNVKLARSLWGNFQPTVLTRLKEFTGKYGF
                     SVAAGDLQLLDGRWYVTHAGLLGLSCRKKCLGIKATLQKALSDPVGNRWVFKAIVYTT
                     PGSKGFVGYGDADPSNTSSLVRGCEMRVAETRAVNRALRKAYGIGLCSVEELGYLTSR
                     PEPAPRNGTQSNHPNGNGSGNGQPRLRDRLCLLIRQHHLDPHLVKRYAADFCGTQEIR
                     EASRDLVESFITHLSEWAGRDRAALLCKLNSYSESKEVHS"
     gene            1211..1654
                     /locus_tag="VNV63_00840"
     CDS             1211..1654
                     /locus_tag="VNV63_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61193.1"
                     /translation="MKRHFPGLHVGSNNGSDLLEGLFLVRVAQASYRWHREKPFFALR
                     FSILEPKEYVGQTLSSRLYCTPKALWKLNWFLRDFGYDNELFQRDEIDEKALLNLTGV
                     VRTSRTTLNGRSFLNFEAFAPAGNWGELRSAAVMAGDGEENGNDL"
     gene            1644..>1909
                     /locus_tag="VNV63_00845"
     CDS             1644..>1909
                     /locus_tag="VNV63_00845"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HXC61194.1"
                     /translation="MIYSHTQISQYLRCPRSYRYRYLDGWKEKDTRAAMVFGRCFEKA
                     LAAYFCREDSAAVLFKEWGVYRDAPLEYGKTDTWDKLVHQGVHLL"
BASE COUNT          427 a          543 c          485 g          454 t
ORIGIN      
        1 acggggccga gtggcttgtc tgcatttaga agattggcgt gttctcgacc agattacccc
       61 tctcctgtca gtaaccactc aatcgacttc ccaaacgcct gactgatcct caaaagaatt
      121 tctgctccca tctccttttc tccgcgttcc acagacgaga gatggccttg actaatgcct
      181 atccgttgag cgaattcctc ctgagtaata tcaaatcccc gaagttcgcg gattcgtcgg
      241 cccaccagtt tccattgaat caaggtcttg cctgcccgcg aggatttgct tgacatcata
      301 atctctttag aattaatata tagctacgga attacacgac atctgcgcat ccattttaac
      361 cgaatctcta aattgccttt ttacattgtc gagacgtgaa ccgcaatgaa agaaatatgc
      421 aatcagggag gacaagcatg agcaagatcg ttgttaataa gcaatcgaat cgcattcgat
      481 caaacgtcaa gctggcccgc tctttgtggg gcaatttcca accaactgtt ctcacgcgtt
      541 tgaaagagtt cacgggaaag tatggcttct cagtcgcggc tggtgacctc cagctcctcg
      601 atggtagatg gtacgtcacg cacgccggcc tccttgggct ctcctgccgc aagaagtgcc
      661 taggaataaa ggcgacttta cagaaggccc tctctgatcc cgtcggcaat cgatgggtct
      721 tcaaagctat cgtttacacg accccgggtt caaagggctt tgtcggttac ggcgatgccg
      781 atccttcaaa cacatcctct ctcgtccgcg gctgcgagat gcgcgtggcg gagacgcgcg
      841 cggtcaaccg ggccctgcgc aaagcctatg ggattgggct ttgctcggtt gaggagctgg
      901 ggtatctcac ctcgcgtcct gagccggccc cgagaaatgg aacccaatcg aatcatccga
      961 atgggaatgg ttccggcaac ggacaacctc gcctccgcga tcgactctgc ctcctgattc
     1021 gccagcatca tctcgatcct catctcgtta agcgttacgc cgccgatttc tgcggcacac
     1081 aggaaatccg cgaagccagc agggatctgg tcgaatcctt catcacccat ctttccgaat
     1141 gggccggccg ggaccgtgct gccttgcttt gcaaactcaa cagttattca gaatccaagg
     1201 aggtgcactc atgaaacgcc atttcccggg tctgcatgtg ggatcaaaca acggtagcga
     1261 tctcctggaa ggattgtttc ttgtccgggt tgcgcaagcc tcctatcgtt ggcatcgcga
     1321 aaaacccttc tttgctcttc ggttttccat cctcgagccc aaagagtatg tgggccagac
     1381 cctttcgagt cggctctact gcactccgaa ggctctgtgg aagctgaatt ggttcctgcg
     1441 ggattttggc tacgacaacg aactgtttca gcgagacgag atcgatgaaa aggctctcct
     1501 caacctgacg ggagtcgtca gaacctctcg aaccacgctc aatggtcgct ccttcctgaa
     1561 cttcgaagcc ttcgcaccgg cagggaattg gggagagctt cgatcggctg ccgtcatggc
     1621 cggtgacggc gaggagaacg gcaatgatct atagccacac ccagatcagc cagtatctcc
     1681 gttgccctcg cagctatcga taccgctatc tcgatggatg gaaggaaaag gacacccgcg
     1741 cggccatggt ctttggccgc tgcttcgaaa aggcattggc agcctacttt tgtcgcgagg
     1801 acagtgctgc cgtcctgttc aaggaatggg gagtctaccg cgatgcgcca ctcgaatatg
     1861 gcaagacgga tacctgggac aagctggtcc atcaaggtgt tcaccttct
//
LOCUS       DATMKH010000072         1069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3562455, whole genome shotgun sequence.
ACCESSION   DATMKH010000072 DATMKH010000000
VERSION     DATMKH010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3562455"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="VNV63_00850"
     CDS             <1..469
                     /locus_tag="VNV63_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61195.1"
                     /translation="AEAAIRLEEYWNELARLYPFSLFCGYMLDSHMKESYAGPLHEIG
                     RTHSDVISTTDDELFRKALDAASKDIFGIPLPEMISLSGQEENPGEHRLPIGQRAMLW
                     IMRHMPSSSGEVLELTRRYYQDLHHKANATISANGTRDGKSVHPVSTPIKSVP"
     gene            643..>1069
                     /locus_tag="VNV63_00855"
     CDS             643..>1069
                     /locus_tag="VNV63_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018294643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HXC61196.1"
                     /translation="MSRTKTKLQPLKKSPTGINGFDEITGGGLPKGRPTIISGGPGCG
                     KTLFAMEFLVKGAVRYGEPGLFVAFEETSEELTKNVASLGFDLQDLIKRKQLWIDFIE
                     IERSEIQETGEYNLEGLFVRLNHAIDSIGAKRVVLDTIEV"
BASE COUNT          272 a          268 c          268 g          261 t
ORIGIN      
        1 ggcggaggca gcgatccggc tagaagagta ttggaacgaa cttgcccgtc tgtacccctt
       61 ttctcttttt tgcggctata tgctggacag ccacatgaag gaatcctatg ccggcccgct
      121 tcatgagatc ggacgaactc attcagacgt catctcaact acggatgatg aactgtttcg
      181 aaaggctctg gatgcggcga gcaaggatat cttcggcatc ccgctccctg agatgatcag
      241 tctctccggt caggaagaga atcccggaga gcaccggctt cccatcggcc agcgggcgat
      301 gctttggatt atgcgacaca tgccttcctc aagtggtgag gttctggaac ttacccgccg
      361 ctactatcag gatttacacc ataaagctaa tgcgaccatc tctgccaatg gtaccaggga
      421 cggaaagagc gttcacccgg tttctacgcc aattaaatca gttccctgac atttttaaat
      481 agcgtcagga gtttcgatcc tcctctgttc caccgtgtag gacaaacacg tacaaaaaat
      541 taagattttc gctgatagat ttctattgcg aactcgctat ataatatttc actcactcaa
      601 tcttcaccta gtcacaggac gttaaaagct aagacgataa agatgagcag aacaaaaacg
      661 aaacttcagc cgctgaagaa atctccgacc ggcattaacg ggtttgatga gattaccggg
      721 ggtggacttc ccaaaggacg cccgacaatc atctccggcg gcccgggatg cggcaagact
      781 ctattcgcta tggagttcct ggtcaaaggg gcggtccgat acggcgagcc tggtctcttt
      841 gtcgcctttg aagagacctc tgaggagctc acgaaaaatg tggcttctct tggattcgat
      901 ctgcaggatc tcatcaaacg caagcaatta tggatcgatt ttatcgagat cgagcgcagc
      961 gagatccagg agacgggcga gtacaatctg gagggtcttt ttgttcgcct caatcatgcc
     1021 attgattcga tcggggcaaa gcgtgttgtc ctggatacaa ttgaagtgc
//
LOCUS       DATMKH010000073         6516 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_37360,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000073 DATMKH010000000
VERSION     DATMKH010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 6516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6516
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37360"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="VNV63_00860"
     CDS             complement(<1..683)
                     /locus_tag="VNV63_00860"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HXC61197.1"
                     /translation="MKRIIILGSTGSIGVNALDVVARFPDRFQVVALAAGSNSERLEE
                     QIRLFRPQIASLMSEEAAAKLRDRCRDLKVNIVSGIGGMIEVATYQEGDLVIAAIVGA
                     AGLVPTLSAIRAKKPIALANKETMVMAGQLVTEEAKRHGVTIFPVDSEHSAIFQSMEG
                     HRRENIRKLILTASGGPLLKTPLAKMRHVTTKQALKHPNWKMGAKITIDSATLMNKGL
                     EVIEARWLF"
     gene            complement(720..1541)
                     /locus_tag="VNV63_00865"
     CDS             complement(720..1541)
                     /locus_tag="VNV63_00865"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HXC61198.1"
                     /translation="MGIKRVITAVVFIPLFYFLVKYLPPVFFFLFVATGIVIGLYEFY
                     RFHYGPVPSPSIRLGLILGALVAAGFYRHDLIEGSLLMIGTVFIILLFHLFFSGNLAS
                     SLIDSSVVLFGLAYVAWFLGYLILIRGFEDGGRAIFFLFLVTWAGDTGAYYVGKSIGR
                     HKLSPVVSPNKTIEGLVGGILTSMMVALLARAWFFPALSRRECLVLGSLFGIIGQIGD
                     LAESMLKRSAGIKDSGSIIPAHGGLLDRADSLIFTAPVFYYYLVLVKGYGHAILI"
     gene            complement(1544..2350)
                     /locus_tag="VNV63_00870"
     CDS             complement(1544..2350)
                     /locus_tag="VNV63_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HXC61199.1"
                     /translation="MGQNNNSTILNSEEAELIALLRNGDLPKHVAIIMDGNGRWAERQ
                     SLPRIAGHRAGIQSVREAVTLCRELGIQVLTMYAFSTENWQRPQGEVSMLMRLLEEYL
                     QRELETMMANGVRFKTIGRIDRLPASVQDWIRKVEEKTVSNKEMVLTIALSYSGRTEV
                     LDAVRCLLKDYDRKLITPEELGEELFSSYLSTDGLPDPDLLIRTSGEMRISNFLLWQS
                     AYTELYFTKTLWPDFRRRDFLLALLDYHQRERRFGLAGEQIQQPRKEKRG"
     gene            complement(2337..4175)
                     /locus_tag="VNV63_00875"
     CDS             complement(2337..4175)
                     /locus_tag="VNV63_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61200.1"
                     /translation="MSRRIKILRLLVAVTFLGIINGMPIESNAKPKASLDEIAKVLAT
                     YFPKLDGKVISVEDQVITINLGKGQGVISGMILRLYRPGEQFLHPLTKEPLGQFEEEI
                     GRVKVSQVDEKTSLVKLLGPATNGGLKVGDGVRLSGARFPVAILPGSSTTNRILLQEF
                     SAALQDTGRFEPVSPEAIAEATLLKRTPDSSGPGDEKQPAAGLDIDYLFQVTTAAGVG
                     ATDMTIKVLTTRDDQPLVTLDAKADLSSEMGLALLQESKPPSVQMNPKQSAVKITLPF
                     EAKHLVLADMDHDGRPEWIISDGIKIRIYHLEKNKPEMIWEEKDENKNNHHLALDAAD
                     LNQDGFPALFITNMIDGNLDSYVIEWHDGQYQKTVKHLPYFLRVWRSLEKTPKLLAQR
                     TSLTEPFAGPIHVMTRSAGGYAEGPVFDLPSGVDLYGSAALDLDGDGVEELIQVDDDD
                     HLLIFRKNGKLLLRTPERYGGYENGFEHVVPATVQVAQEKQNFVKIKGRIELMNNPQG
                     GSWIVLTKNIPLTYLVSRSRGYHESEVYGLDWDGADSTEAWKIPLPEQVIEDVQLSDV
                     MGEGQPQVILLLRPGPSLTTFKSFFGKQSELRIYRVPGGAEKHGAK"
     gene            complement(4292..4696)
                     /locus_tag="VNV63_00880"
     CDS             complement(4292..4696)
                     /locus_tag="VNV63_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61201.1"
                     /translation="MKITLAVLADYANITREGKLNILGIFDVIHAQDFPMTHPQMQLV
                     LRFEADIAEAGRTKKLEIKLMDDDGKTLFGLNGNFTLGQGQAGEVMVSNQILTINMMN
                     FEKPGNHEFKILINDELKTEVPLKVVKYTPPS"
     gene            complement(4700..5278)
                     /locus_tag="VNV63_00885"
     CDS             complement(4700..5278)
                     /locus_tag="VNV63_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61202.1"
                     /translation="MISDTDFFPAKAEIIRKLHEDSMRLRDQIRMECKKTPLSLAPAG
                     TDFETGQLVKGEKFQDFPYVYLDFPKFFSRDEMFTFRSFFWWGHSLIFCWFLSGPRLD
                     EYRKRLLACHKTISHQTVFLSTAGTPWEWGLEKPHAEFLDRIKKERLKEILAERTFLK
                     FGCSMPLKFLETPDAILREGLRVFNFLKSIVE"
     gene            complement(5317..>6516)
                     /locus_tag="VNV63_00890"
     CDS             complement(5317..>6516)
                     /locus_tag="VNV63_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020401725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HXC61203.1"
                     /translation="RNDFLKYLCQTSEEPIGIEVDHARGCLIVDHNRKEHLDFISGIG
                     VANIGHAHPKIVKAICDQAKRFLHVMVYGEYLQQPQVALAKRLSLVVPKPLSVTYFTN
                     SGTEAVEGALKTAKKFTKRTRFVAFEGSFHGDTTGALSVTGREVYRKPFEPLLPNVRF
                     LPFNATHALQTIDETTAAVIVEPIQSEAGVRVPDDDFLPALRKRCDRTGTLLIFDEVM
                     TGLGRTGKMFASEHWNVVPDLLILAKALGGGMPLGAFIGRPEVMNVLSVDPPLSHVTT
                     FGGHPVSCAAGLAALEVLIGESLAKQAGQKGRRLREGLKRVAEYGGIRDVRGKGMLVG
                     LEFDHPGLTRRFVLACRKAGLLLGWTLHSDTVVRLAPPLVISQKELDRGLMIMQDALR
                     KSVKGKA"
BASE COUNT         1592 a         1855 c         1549 g         1520 t
ORIGIN      
        1 tcaaacagcc agcgcgcctc gatcacctca agtcccttat tcattagggt cgccgaatca
       61 atcgtgatct ttgcgcccat cttccagttg ggatgtttta aagcctgctt cgtcgtcacg
      121 tgtcgcatct tggccagtgg cgttttcagc agcggtcctc cggaggcggt caggatcagc
      181 tttcggatat tctctcgccg gtgtccctcc atggattgaa agatcgcgct gtgctcgctg
      241 tccacaggga agatcgttac cccgtgcctc ttcgcctctt cggtcacaag ctgaccggcc
      301 atgaccatgg tctccttatt ggccagggca atcggcttct tggcccgaat cgctgaaagc
      361 gtcggcacga gaccggccgc tcccacaatg gccgcaatga cgagatcgcc ctcctgatag
      421 gtggcgacct cgatcatccc gcctattccg gagacgatat taactttaag atcgcggcag
      481 cgatcccgga gcttggccgc agcctcttcg ctcatcaacg aagcaatttg cggtcgaaat
      541 agccggatct gttcttccaa tcgttcgctg ttcgatcctg ccgccagggc gacaacctgg
      601 aatcggtccg gaaacctggc aaccacatcc agtgcattga ccccgatcga accggtggaa
      661 ccgaggatga tgatccgctt catcatgtaa tcgcacactt tcgaattaag accgtgctgc
      721 taaatgagaa ttgcatggcc ataccccttg actaaaacca ggtagtaata aaaaaccggt
      781 gcggtaaaga tcaggctgtc cgcgcggtcc agcaaccctc catgagcagg gatgattgag
      841 ccggaatctt tgatccccgc gctccgtttg agcatggact ctgccagatc cccaatctgc
      901 ccgataatgc caaagaggga ccccaagacc aaacattctc ggcggctcaa ggccgggaaa
      961 aaccaggcgc gggccagcaa agcgaccatc atgctggtca atatccctcc caccaaacct
     1021 tcgatggttt tattcgggct caccacgggc gaaagcttgt gccttccgat cgatttcccc
     1081 acataatagg cgcccgtatc ccccgcccaa gtcaccagaa aaagaaaaaa gatcgccctc
     1141 cctccatcct caaaaccccg gatcagaatg agataaccca agaaccaagc cacatacgcc
     1201 aaaccgaaca gcaccaccga cgagtctatg agactggatg cgaggtttcc ggaaaagaag
     1261 agatgaaaca acaaaataat aaagaccgtg ccgatcatga gcagagaacc ttcgatcaaa
     1321 tcatgacggt agaaaccggc agcaacgaga gctcccaaga tcaggccaag tcggatcgaa
     1381 ggggagggaa ctggaccata atggaaccgg tagaactcat aaagaccgat cacgattccc
     1441 gtcgctacga acagaaagaa aaacacgggc ggcagatatt tcaccagaaa gtaaaacaaa
     1501 ggaatgaaaa caaccgctgt gatcacacgt tttattccca tccctatccc cttttttctt
     1561 ttcgaggctg ctgtatctgc tctcccgcca atccgaatcg tcgctctcgc tggtgataat
     1621 ccaaaagggc gagcaggaaa tcacggcgac ggaagtccgg ccaaagggtt ttggtgaagt
     1681 agagttcggt ataggccgat tgccagagaa ggaaattact gatccgcatt tcgccgctgg
     1741 tacggatgag aagatccgga tcgggcaatc cgtccgtgga gagataactt gagaacagtt
     1801 cttcgccgag ctcttccggc gtaatcagct tacggtcata atctttcaga aggcaccgga
     1861 ccgcatcgag cacttccgta cggccgctgt agctcaaggc aatcgtcagg accatctctt
     1921 tattagaaac ggtcttctcc tccacctttc ggatccagtc ctggaccgaa gcgggaagac
     1981 ggtcaatccg gccgatggtc ttaaaacgaa ccccgttggc catcatcgtc tccaactctc
     2041 gctgaagata ctcttccagc agacgcatga gcatcgaaac ctcgccttgc gggcgctgcc
     2101 aattctctgt cgagaaggcg tacatcgtca agacctggat tccgagttca cggcagaggg
     2161 tgaccgcctc ccgcaccgac tggatcccgg cacgatgacc cgcaatcctg ggcagactct
     2221 gtcgttctgc ccaccggccg ttgccatcca tgatgatggc cacgtgcttg ggtagatccc
     2281 cgtttcgtaa gagagcgatc agctccgctt cttccgagtt taaaatcgtt gagttgttat
     2341 tttgccccat gcttctccgc tccgccggga acccgataaa tacgcaactc ggactgcttg
     2401 ccaaaaaaac ttttgaacgt ggtcaggctc gggcccggtc tgagcagcaa gatcacctgc
     2461 ggctggccct ctcccataac atcgctcaac tgaacatcct caatcacctg ttccggcaac
     2521 gggattttcc aggcttctgt cgagtcagca ccgtcccagt ccagaccgta cacctcgctc
     2581 tcgtgatagc cacgggaacg gctcacgaga taagtaagtg ggatgttctt cgtcagaaca
     2641 atccatgatc cgccttgggg attattcatg agctcgattc gccctttgat tttaacaaaa
     2701 ttttgtttct cctgcgcgac ctgaacggtt gcgggaacga catgttcaaa gccgttctcg
     2761 taaccgccgt accgctccgg cgtccggaga agaagcttgc cattttttct aaatatcaag
     2821 agatggtcat catcgtccac ctggattaac tcctcaacac catcgccgtc aagatccaat
     2881 gcggctgaac cataaagatc cacaccggaa ggcaaatcaa agaccggacc ttctgcatag
     2941 ccgccggcgg atcgtgtcat tacatgaatg ggacctgcaa aaggctctgt gagactcgtg
     3001 cgctgtgcaa ggagtttggg tgttttttca agcgaacgcc atacgcgcaa gaaataagga
     3061 agatgtttga cggtcttttg atactgccca tcatgccatt cgatcacata agagtccaga
     3121 tttccatcaa tcatgttcgt gataaaaagc gccgggaaac catcctgatt cagatctgcc
     3181 gcatccaagg ccaggtgatg gttgtttttg ttttcatcct tctcttccca gatcatttcc
     3241 ggtttattct tctcaagatg atagatccga atcttgatcc cgtcgctgat gatccattcc
     3301 ggtctcccat catggtccat atcggccagc accaggtgct tcgcctcgaa gggcagcgtt
     3361 atcttgaccg cagactgttt tggattcatc tgaacagaag gaggtttgga ctcttgaagg
     3421 agggctaacc ccatctccga agaaaggtcc gccttcgcat ccaaggtgac caagggctga
     3481 tcgtctcgag tggtgagtac cttgatcgtc atatcggtgg cacctacccc tgcagcggtc
     3541 gtgacctgaa agagatagtc tatatccaag cccgcagcgg gttgtttttc atccccggga
     3601 ccggatgaat ccggtgtccg cttcaagagc gtcgcctctg caatcgcctc cggactcacc
     3661 ggctcgaacc gtccggtatc ctgtaaagcc gcagaaaatt cctggaggag aatacgattc
     3721 gtagtggaac tgccgggcag gatcgctacc gggaacctgg caccgctgag ccgtacacca
     3781 tctccaacct taagcccgcc gttagtcgcc ggtccaagaa gtttgaccag agacgtcttc
     3841 tcatccacct gactcacctt cacccggccg atctcctctt caaattgccc tagaggctct
     3901 ttcgtcagag gatgcaagaa ctgttcaccc ggccggtata gtcttaagat catcccagag
     3961 ataactccct gcccttttcc aagattgatc gtgataacct gatcttcgac cgagatgact
     4021 ttcccgtcaa gttttgggaa ataggtagcc agcaccttgg cgatctcatc caggctggcc
     4081 ttcggcttgg cattgctttc aatcggcatc ccattgatga tgccaaggaa ggtaacggcc
     4141 acaagcagtc gtagaatttt tatacgccga ctcatcttta taaagaatcc ttataaaata
     4201 aataatcctt caagattaat atcttaaagc atatagtcta atgggtcaac cgataaatgt
     4261 caaacgataa gataggtata ggataaaggg cttatgaagg cggagtatat ttaacgactt
     4321 ttaatggcac ttctgtcttt agctcatcat taatgaggat tttaaactca tgattgccag
     4381 gcttctcaaa attcatcatg ttgattgtca gtatctggtt tgaaaccatg acttctcctg
     4441 cttgaccttg tcccagcgtg aagttgccgt ttagtccaaa caatgtttta ccgtcatcgt
     4501 ccatgagttt gatctcaagt tttttagttc ttcccgcctc cgcaatatct gcttcaaatc
     4561 tcaagacaag ctgcatctgt ggatgggtca tcggaaaatc ctgcgcatgg atcacatcaa
     4621 agattcctaa gatgttgagt tttccttctc ttgtaatgtt cgcgtaatcg gctaaaaccg
     4681 caagagtgat tttcatggtt tactccacaa tcgatttgag gaaattaaag acgcgcagtc
     4741 cttctcgaag gattgcgtcc ggagtttcta aaaactttag tggcatcgag cagccaaatt
     4801 ttaagaatgt ccgctccgcg aggatttctt ttaaccgctc cttcttgatc cgatccagaa
     4861 actcggcgtg cggtttctct aaaccccatt cccacggggt gccggcggtg gaaagaaaga
     4921 cggtttgatg ggaaatcgtt ttatgacagg caagcagcct ctttcggtat tcatccaggc
     4981 gaggtcccga taaaaaccag cagaagatca ggctgtgccc ccaccaaaag aacgaacgga
     5041 aggtgaacat ctcgtctctt gagaaaaatt tggggaagtc cagatagaca tagggaaagt
     5101 cctggaactt ttctcccttc accaactggc cggtttcaaa gtcggtcccg gcaggtgcca
     5161 ggctcagcgg agtcttctta cattccatcc gaatctgatc gcgcagcctc atggagtctt
     5221 catggagttt gcgtatgatc tccgctttcg caggaaaaaa gtccgtgtca gagatcatgc
     5281 gcatctcctc ggatgtgaaa cggcctccaa tcgtcatcaa gcttttccct taacagactt
     5341 cctcaaggca tcctgcataa tcatgaggcc ccgatccagt tctttttggc tgatcaccaa
     5401 cggcggcgcg agccgcacaa cggtatcgga atgaagcgtc cagcccagca agaggcccgc
     5461 cttccggcaa gccaaaacaa aacggcgggt cagccccgga tgatcgaact cgaggccgac
     5521 cagcattcct tttccgcgaa catcgcggat acctccatac tcggcaactc ttttcaaacc
     5581 ctcccgaagt ctccgaccct tctgcccggc ctgcttagca agactttctc cgatcaatac
     5641 ctccagcgcc gccagcccgg ccgcgcacga gaccggatgg ccgccaaagg tggtaacatg
     5701 agagagagga ggatccactg agagtacatt catcacttcc ggccttccga taaaggcacc
     5761 cagcggcatt ccgccgccca gcgccttagc caatatcaag agatccggca cgacgttcca
     5821 atgttcggac gcaaacattt ttccggttcg gccaagacct gtcatcacct catcgaagat
     5881 cagcagggtc cctgtacggt cgcaccgctt ccgcaaggca ggtaaaaaat cgtcgtccgg
     5941 tacacgcacg ccagcttcgc tttgaatcgg ctccacgatg accgcggccg tcgtttcatc
     6001 aatcgtctgg agggcatgtg tggcattaaa cggcaggaac cggacattcg gaagaagcgg
     6061 ctcaaacggt tttcgatata cctctcggcc ggtcacggac aacgcaccgg tcgtatcccc
     6121 atgaaagctc ccctcaaagg ccacgaatcg cgttctcttt gtaaatttct tcgcggtctt
     6181 caacgccccc tcaaccgcct ccgtcccgct gttcgtaaag taggtcaccg acaacggctt
     6241 aggcaccaca agcgagagcc gtttggccag cgcaacctgc ggctgctgaa gatattcgcc
     6301 atacaccatc acatggagaa aacgtttcgc ctgatcgcag atcgccttta ctattttcgg
     6361 gtgcgcatgg ccgatattgg ccacgccgat gccggatatg aagtctagat gctcttttcg
     6421 gttgtgatct acaatcaggc agccgcgggc atgatccacc tcgatcccga ttggctcctc
     6481 tgaggtctga cagaggtatt tcagaaaatc atttct
//
LOCUS       DATMKH010000074         1565 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_999922,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000074 DATMKH010000000
VERSION     DATMKH010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_999922"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..>1565
                     /locus_tag="VNV63_00895"
     CDS             90..>1565
                     /locus_tag="VNV63_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61204.1"
                     /translation="MFIRRELADYTIPQSVHRWKNWLWMSVGLGLLVMTTFLFANIVQ
                     SLVTDAKISFNVTNFFSLLNNYSFVGFIALAALALGYFFLSQILFQLIGYLIRSLSIV
                     LYIITATMGLLVLTFIQRPDMVELDIYVLIWLLGYVWLMQRRIFFDLRFRLNVSEVLF
                     WLFIFSISISAVILFENRKIGEAQRREMAERLGKQSDPSGETLLSINFSYIDNDFLTA
                     NFSRFRDQYANQLIKDSIGNRNWTTYQENFDTRLYTFDGDEKPLYNEPQTSYDTLNTT
                     FTVQGKSTDIPDLRYVQRSFDRFAYIYKKTVRNDTSGKVVGYLFILSEPKGQKSRGDA
                     LVPELFHERSEDQDLPEYYAIYNKGELVTPGGDYQFATHLTEGQDTSKADFVHVKKYG
                     YDELWHRVSDGNFVVVVKRDNSSIEDITLFAYLFSAFLFLVALFRGTALLIWSRLHWS
                     QLKTYWQMNIRSQIHTTIIFISLFSFVVIGVATIIFFINRYN"
BASE COUNT          340 a          365 c          420 g          440 t
ORIGIN      
        1 tgttcgatcc taccatctac agttcaagta ttatcctcaa ttcccttggc gatctggtca
       61 tcaattcctt cctgttctgt tggatcatca tgttcatccg gcgggagctg gccgattata
      121 cgatcccgca gtcggtgcac cggtggaaaa actggctttg gatgagcgtt ggtctgggct
      181 tgctggtgat gacgaccttc ctctttgcca atatcgtgca gtcgctggtg acggatgcga
      241 agatctcgtt caatgtcacg aacttcttca gcctgctgaa taactatagt tttgtagggt
      301 tcattgcgct ggcagcactg gcgcttggtt atttcttcct atctcagatc ctgttccagt
      361 tgatcggcta tctgatccgg tccctgtcga tcgtgcttta tatcatcacg gcaacgatgg
      421 ggctgctggt gctgaccttt atccagcgac cggatatggt agagctggat atctatgtgt
      481 tgatctggtt gctgggttat gtctggctga tgcagcggcg gatcttcttc gatctgcggt
      541 tccggctgaa tgtctcggag gtgctgttct ggctctttat tttctccata tccatttcgg
      601 cggtgatcct gtttgagaac cggaagatag gagaagcgca gcggcgggaa atggctgaac
      661 ggctgggaaa acagtccgat ccttccggtg aaacattgct aagtattaat ttcagctata
      721 tcgacaatga ttttctgacg gcgaattttt cccgtttccg cgatcaatat gccaatcagc
      781 tgatcaaaga cagcatcggt aacaggaatt ggactaccta ccaggagaac ttcgatacca
      841 ggctgtatac ctttgatggg gatgagaaac cgctgtataa cgagccgcag acttcttatg
      901 atactttaaa cacaactttc acggtacagg ggaagagcac ggacatcccc gatctgcgat
      961 atgtgcagcg gtcgtttgac agatttgcgt atatctacaa gaaaacggtc cgcaatgata
     1021 cgagtgggaa agtagtgggg tacctcttca tcctgtcgga accgaagggc cagaaaagcc
     1081 ggggggatgc gcttgtgccg gagctctttc atgagcggag cgaggatcag gatctgccgg
     1141 aatattatgc catttacaat aaaggcgagt tggtgactcc cggcggggat tatcagttcg
     1201 ccacccatct gactgagggt caggacactt ccaaggcaga ctttgtgcat gtcaaaaaat
     1261 acgggtatga tgagctatgg caccgcgttt cggatggcaa cttcgtggtg gtggtgaagc
     1321 gggacaattc atcgatcgaa gatattacgc tgtttgctta tctgttcagc gccttcctgt
     1381 tcctggtggc gttgttccgc ggcaccgcgc tgctgatctg gtcgcggctt cactggagcc
     1441 agctgaagac ctactggcag atgaatatcc gttcgcagat ccatacgacg atcatcttta
     1501 tcagtctgtt ctcttttgtg gtcatcgggg tagcgacgat catctttttt atcaaccggt
     1561 ataac
//
LOCUS       DATMKH010000075         3090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4207507, whole genome shotgun sequence.
ACCESSION   DATMKH010000075 DATMKH010000000
VERSION     DATMKH010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3090
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4207507"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="VNV63_00900"
     CDS             complement(<1..443)
                     /locus_tag="VNV63_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61205.1"
                     /translation="MADTNTSTTTLNNLLLAWFSRKIIATLVPKTPLIEFAQRDELPL
                     RTGTTAQFNGWTRIKGASSGLSEGTANSLIALSSRKVTGTIAGYGRGVKLTDLVTMTT
                     IFDAVNGAMERLADSAAETVEQMCQMGIFKADIQKNQNTTTNISS"
     gene            complement(516..1511)
                     /locus_tag="VNV63_00905"
     CDS             complement(516..1511)
                     /locus_tag="VNV63_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61206.1"
                     /translation="MEALETAKPAEMSLEKPPPFVASQSGAVEKAPTANEEEQLRALF
                     NDAHARGLDEKEMMVNVPKAQANGPEQPPVPPTPAPASIPQKFMKPDGTVDEDKLKAS
                     SERLNEVVEQKQKTVDELLLEYQEREKKLHSLGQQKAEIQAQMPPVPPPVPVQQQSDP
                     QQLQQRIMQDYQQNPLDTTIKLAEAIAATKVAPILQRFEQDEEQRRLAGMRQNVANLA
                     QSDPRFLDPRLNAMVNQVLDEDPQMMRLKNPHKAAWNEVKERLRLGEPNQVQAQPSNA
                     MPTLARGAPPSVSTLSGPLSPQSARQVSQQVSPYSPEGKAFEEQLKELTKDVWQY"
     gene            complement(1543..1911)
                     /locus_tag="VNV63_00910"
     CDS             complement(1543..1911)
                     /locus_tag="VNV63_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61207.1"
                     /translation="MRKFSAWIKKVLEPAKSNFEDGKIEEVMQALGDETIRYYWMKSL
                     EEEIWTLNIKLDRMMDENADTEWRQTAMRRRAILFVLNKILEAKELIESEREDRENQN
                     RKAERMASLTAVPLDLRQSE"
     gene            complement(1898..2086)
                     /locus_tag="VNV63_00915"
     CDS             complement(1898..2086)
                     /locus_tag="VNV63_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61208.1"
                     /translation="MMNDDDDELKEGLKKSMEEPAKKKDDDAYGTFVRNQMEKMAPSG
                     WLAQRKKDYEGPHGNEEV"
     gene            complement(2083..>3090)
                     /locus_tag="VNV63_00920"
     CDS             complement(2083..>3090)
                     /locus_tag="VNV63_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61209.1"
                     /translation="AVFEKAIVNRKDLNSQPGGIVRIKDQISDDIRKVLTPLEFPDVS
                     ASAYKETMEIERQIQERTGANRVTMGSNGGVRDSNQTLGGMELLKQMFNERLAAYGMI
                     IESQFLTEACERIYSLIYGNLQPDDMKSILGDTPVQIGTIQPPPPPPPVPGQLPLPQA
                     PPIPHMVPRYLAFVFVPPEMVAESYRFKPMGIFSMENKIVKSAQVMDAIKVGTMLPPG
                     QMNISSALQFVLEKLQTIPEAKAWFPPYPDMAGIPSGAPQMPMPPPGMGGPPPPPGQG
                     APPPGMGPLGPEAGHAPPNKIPAQASPSAATAAPGMKSGPRGNQPSFLPRPIRRQPVA
                     P"
BASE COUNT          670 a          862 c          733 g          825 t
ORIGIN      
        1 taagaggaaa tattcgtggt ggtattctgg ttcttctgaa tatccgcctt gaaaatcccc
       61 atctgacaca tctgctcaac agtttccgcc gcagagtcgg ccaaccgctc catcgcgcca
      121 ttgacggcat caaaaatggt cgtcatcgtc acgaggtcgg tcagcttcac gccgcgtccg
      181 tatccagcaa ttgttcccgt taccttacga gacgaaagcg caatcagaga attcgctgta
      241 ccttcagaaa gtccagacga agctcctttg atgcgggtcc atccgttaaa ctgcgccgtt
      301 gtaccagttc ggagcggaag ttcatcccgt tgcgcgaatt cgatcagcgg cgttttcgga
      361 accagcgtcg caataatctt ccggctaaac caagccaaga gaagattatt tagcgtcgtg
      421 gttgatgtat ttgtatctgc catttaatct atttgggcct tgggcctgtt acggcttatc
      481 tcccaaatcc tttgagcaca tatccttagt ggacgctaat actgccaaac atccttcgtc
      541 aattccttca attgctcttc aaaggctttg ccttcaggag aataaggact cacctgctgg
      601 gatacctgac gagccgactg aggcgataaa gggcctgaca acgttgagac agacggtggt
      661 gcgccacgcg cgagcgtcgg catggcgttg ctgggttgtg cttgaacctg attcggttca
      721 cccaatcgca accgttcctt gacttcattc caagcggctt tatgagggtt cttcaagcgc
      781 atcatctgcg ggtcttcatc cagaacctga ttcaccatcg cattcaagcg cggatcaaga
      841 aaacgcggat cagactgggc aagattcgcc acattctgac gcattccagc caaacgacgc
      901 tgttcctcgt cctgctcaaa cctctgaaga attggagcca ccttcgtagc ggcaatcgct
      961 tccgcgagct tgatcgtcgt atccagaggg ttctgctgat agtcctgcat gatgcgctgc
     1021 tgtaactgct gaggatcact ttgctgttga acaggaaccg gaggtggaac aggcggcatc
     1081 tgcgcctgaa tctccgcttt ctgctgacca agagaatgaa gtttcttctc acgctcctga
     1141 tattccaata acaattcatc cacagtcttt tgtttctgtt ctacaacctc attaagtcgc
     1201 tcggaagaag ccttgagctt atcttcgtcc actgtcccat ccggtttcat gaacttctga
     1261 ggaatggatg ccggtgctgg tgtcggtggc acaggaggtt gttcaggacc attggcctga
     1321 gccttcggca cgttcaccat catttccttt tcatccaatc ctcgtgcgtg agcatcgttg
     1381 aacaaggccc gaagctgttc ctcttcgttc gccgtcgggg ctttttctac agctcccgat
     1441 tgtgacgcta cgaaaggtgg cggcttctcc aagctcatct ctgccggttt cgctgtttct
     1501 aaggcttcca tttatttctc cttatgtgag tccctcgcgg gattattcac tctgtcgcag
     1561 atcaagagga accgccgtaa gcgaagccat acgctcggct ttccggttct ggttctctcg
     1621 atcctctctt tcggattcga ttaactcctt cgcttccaaa atcttgttca acacaaacaa
     1681 aattgcccgc ctgcgcatcg ccgtctgccg ccattccgta tcagcatttt catccatcat
     1741 ccggtcaagt ttgatattca gggtccagat ttcctcttcc agagacttca tccagtaata
     1801 tcggatcgtc tcatcaccca aagcctgcat cacctcttcg atctttccat cctcgaagtt
     1861 cgatttggca ggttccaaca ccttcttaat ccatgcgcta aacttcctca ttgccatgcg
     1921 gcccctcata atcttttttg cgctgagcta accaaccact aggagccatt ttttccattt
     1981 ggtttcggac aaatgttccg taagcgtcat cgtctttttt cttcgctggc tcttccatgc
     2041 tcttcttcaa accttctttg agttcatcgt cgtcatcgtt catcatggcg caacgggttg
     2101 cctccttatc ggtctgggta agaaactggg ttgattacca cgaggtccgg atttcatgcc
     2161 gggagcagca gtcgcggcag aaggactagc ctgtgccgga atcttatttg gcggtgcatg
     2221 gcctgcttcc ggccctaatg gacccattcc cggaggtggt gcgccttgtc ccggaggcgg
     2281 agggggtcca cccatccccg gcggaggcat cggcatctgc ggcgcacccg aaggaatacc
     2341 tgccatatcg ggatagggcg gaaaccacgc cttggcttcg ggaatcgtct ggagcttctc
     2401 caaaacaaac tgaagcgcag aggaaatgtt catctggccc ggtggaagca tggttcccac
     2461 cttgattgca tccatgacct gcgctgattt gacaatcttg ttctccatcg agaaaatacc
     2521 catcggcttg aatcggtagc tctccgctac catctccggc ggcacaaaga caaaagctaa
     2581 ataacggggg accatgtgag gaatcggcgg agcttgcgga agaggcaact gaccgggaac
     2641 cggaggtgga ggcggtggcg gctgaatggt cccaatctgt acgggggtat cgcctaaaat
     2701 ggatttcatg tcgtcgggct gaagattgcc gtaaatgagc gaataaatcc gttcacaagc
     2761 ctccgtcaag aactggcttt caataatcat gccgtaagcc gccaaccgtt cattgaacat
     2821 ctgtttcaga agctccatgc cacccagtgt ttgattggag tctctcacgc caccattcga
     2881 ccccatcgtg accctgttgg ctcctgtacg ttcctgaatc tgccgctcaa tttccatcgt
     2941 ttctttgtaa gcagaagccg atacatccgg aaactcaagt ggtgtcaaga ccttgcgaat
     3001 gtcatctgaa atctggtctt tgatgcggac aatgccaccc ggttgactat tcaagtcttt
     3061 acgattgaca atcgcctttt cgaacacagc
//
LOCUS       DATMKH010000076         1024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4207510, whole genome shotgun sequence.
ACCESSION   DATMKH010000076 DATMKH010000000
VERSION     DATMKH010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4207510"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(421..711)
                     /locus_tag="VNV63_00925"
     CDS             complement(421..711)
                     /locus_tag="VNV63_00925"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HXC61210.1"
                     /translation="MKRLNRDAWIGRSVLKLVEKRAKASGTLKRVCCHSFRATDVTEY
                     MNSGGTIEIAQRIAGHTSPSTIRIYDRSGDQLTLEEIERVQIGKINIVEFPA"
BASE COUNT          227 a          277 c          259 g          261 t
ORIGIN      
        1 aactccaggg ttttgctttt tgtaaacttt gaataacaca tcgagtgcgg cagcctcgcc
       61 gccggaagtc caccaggaaa agacttccag ctttctttcg tcagccgctt gagggaaaag
      121 aggcgctgcc accgtggtga agagtgcaac gcacgccaag aatcggacta aattgcgcac
      181 cttattaaac ggatgtcctc gatttacttg agttctcaac tcgcgcaaac tcttctctca
      241 actttgtagt cgaggtgttt ctcccgcaaa aatgatccag tgggttattt ttatcacgac
      301 ggatttcggt gaacatgaga ctaagcgcca ctgatccacc ccgccggcgg ctggatgcag
      361 acgtcgccac cacatcgggc aattgcgacc ttctggggtc aatgcccggt aaagaaaggc
      421 tcaggcggga aattcaacta tgttgatctt cccgatctgc actctctcaa tctcttcaag
      481 ggtcagctgg tccccggatc gatcataaat cctgatggtc gacggactgg tatgaccggc
      541 gatccgctgc gcgatctcga ttgtgccgcc cgaattcatg tattcggtga cgtccgtcgc
      601 acggaagctg tgacaacaaa ctcgtttcag ggttccgctg gccttcgccc gtttttcgac
      661 cagcttcaag acactccgac cgatccaggc gtctcgattc aatcgtttca cgattcttgc
      721 cgaaggaagg aaaaagtggg gcacttggat ttgatgccag gtgagagcgt tcaagccaaa
      781 gatcgaccgc ttctttagct tttgagtgca ccggtacttc gtgttctttc cctcgggggt
      841 tagcccaaca aaggtacgta aagtcccaca gaaatctctt tagatcccca cgctctggac
      901 cgcatcctgg tacgctatcg accgattcga gtgacaggtg ttggtagacc tcaaggctct
      961 ttttgctttc gtggcccgat atcagctgaa tctgtgcgtc ggtcaggcct ttggatgtca
     1021 ggta
//
LOCUS       DATMKH010000077         1883 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1000273, whole genome shotgun sequence.
ACCESSION   DATMKH010000077 DATMKH010000000
VERSION     DATMKH010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1000273"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="VNV63_00930"
     CDS             <1..612
                     /locus_tag="VNV63_00930"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HXC61211.1"
                     /translation="PMEAAISAIQTESSYSFVAFLRDISERKQLEEHLRQIGLHDALT
                     GLPNRILLTDRLNQALARTRWHKRLVAALFLDLDSFKMINDTLGHEIGDRLLKAVSER
                     LTASLRDGDTVARLSGDEFVIILADVAKVEDIPPIAQKILHILAEPIQIDKHELKITT
                     SVGISFFPDDGELVETLLKKADTAMYQAKKEGKNRFKFYSSPF"
     gene            655..1458
                     /gene="mazG"
                     /locus_tag="VNV63_00935"
     CDS             655..1458
                     /gene="mazG"
                     /locus_tag="VNV63_00935"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HXC61212.1"
                     /translation="MSRSFDRLTKILIRLRSRRGGCPWDLKQTPDSLKPFLIEEAYEV
                     IEAIESKDNARVIEELGDLLYQVLFHAQIARERGRFTIRDVLKATADKMVRRHPHVFG
                     RDRGARRGRTAAEVLVRWEELKAEEKGNRHRTSVLDGIPKQLPALLRAQQLQARAARV
                     GFDWPAIEPVLKKVREELREFEEARKSRSKIRMEEELGDVLFALVNLARFLKINAEDA
                     LRKTSQRFIQRFQYIEQAAAKKRRPVQDLSLSEMDRLWEEAKQLPGNSR"
     gene            1536..>1883
                     /locus_tag="VNV63_00940"
     CDS             1536..>1883
                     /locus_tag="VNV63_00940"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HXC61213.1"
                     /translation="MPQFAYRIAKGDGTILEDRTEAEDEVTLRQQLEGKGYLVFSIRK
                     AAGFSLPQIRFDFKSKLTPREFLAFNQEFLALIKAGLPIIRALDILVDRVELTSFREA
                     LRGIRQEIKGGSSI"
BASE COUNT          456 a          493 c          517 g          417 t
ORIGIN      
        1 cccatggagg cggccatttc tgcgatccag accgaaagct cctactcctt tgtggctttt
       61 cttcgcgaca tctcggaacg caaacaactg gaagaacatc tccggcagat cggcctccat
      121 gacgccctaa ccggtcttcc aaaccgaatc ctcttgacgg atcggctgaa ccaggccttg
      181 gcccggaccc ggtggcataa acggctggtg gctgctctat ttctggatct cgatagtttc
      241 aagatgatca atgacacgct gggccatgaa ataggcgacc ggttgctgaa ggccgtgtcc
      301 gagcggctta cagcaagtct gcgtgacggc gacaccgtcg cccggttgag cggggatgaa
      361 tttgtaatta ttctcgccga tgttgcaaag gtggaagaca tcccgcctat cgcacaaaag
      421 atactgcata tcctcgcaga gccgattcaa atagataaac atgaactcaa gattacgacc
      481 agcgtcggaa tcagtttttt cccggacgac ggcgagttag tggaaaccct gctgaagaaa
      541 gcggacaccg ccatgtatca ggcaaaaaag gaggggaaaa ataggttcaa gttctattcc
      601 tcgccttttt agagttgtga agaccgttca aacgatgtat aattctcccc cgctatgtca
      661 agatcatttg atcgtctcac caagatcctg attcgtctgc gcagtcgtcg cggcggctgt
      721 ccctgggatt taaaacagac gccggattct ttaaaaccgt tcctgatcga agaggcctat
      781 gaagtcatcg aggcgatcga gtccaaagac aatgccaggg tgatcgaaga actgggagat
      841 ctcttgtacc aggttttgtt tcatgcccag attgccaggg aacggggacg attcacgatc
      901 cgggacgtct tgaaggccac ggcagacaag atggttcgtc gtcatcccca tgtcttcgga
      961 cgcgaccgag gcgcccgccg cgggcggacc gcagcggagg tgcttgtccg ttgggaggag
     1021 ctgaaagccg aagagaaagg aaatcgtcac cggacttcgg tgctggacgg tattcccaag
     1081 cagctcccgg ctctgctccg cgcgcagcag ctccaggcac gtgcggcccg tgtggggttt
     1141 gactggcccg ccattgagcc cgtattgaag aaggtgcgtg aagagctccg cgaatttgaa
     1201 gaggcccgaa aaagccgatc gaaaatccgc atggaagaag agctgggcga tgtcctgttc
     1261 gccttggtca acctggcccg cttcctcaaa atcaatgcgg aagatgccct gcgcaagaca
     1321 agccagcgtt tcatccagcg ctttcaatac atcgagcagg ccgcagcgaa gaaaagaagg
     1381 cctgtccaag atctatcgct ttccgagatg gaccggctct gggaagaggc aaagcaactg
     1441 ccgggaaatt caagatagcc cgaagcagga cgcttgcttt cttccccttc ttaacatata
     1501 cttgtagcaa ttataatcag ttggagatga agtagatgcc gcagtttgcc tatcggattg
     1561 cgaaaggaga cggcacgatc ctggaggatc gtaccgaggc ggaggatgaa gtaaccttgc
     1621 gtcaacagct ggaagggaaa ggttacctcg tcttctcgat tcggaaggct gcgggctttt
     1681 ctcttcccca aatccgtttt gactttaaat ccaaacttac gcctcgggaa tttctagctt
     1741 tcaatcagga atttctggcc ttgattaagg ccggcctccc gatcatccgg gccttggata
     1801 ttctggtgga ccgggtggaa ctcacttctt ttcgcgaagc attacgtgga atccggcagg
     1861 agatcaaagg cggctcttca atc
//
LOCUS       DATMKH010000078         1338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1960830, whole genome shotgun sequence.
ACCESSION   DATMKH010000078 DATMKH010000000
VERSION     DATMKH010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1960830"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="VNV63_00945"
     CDS             complement(<1..307)
                     /locus_tag="VNV63_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61214.1"
                     /translation="MDIKRTQLIAQLRPFKMIGTFVAISILFITACGTIPVKGRASVD
                     AKDSTNIRHSKTIPKSAQASQEQKLPAPVLNPLDAAPSASGKDSKSQFFPSLPEDHYT
                     "
     gene            complement(646..>1338)
                     /locus_tag="VNV63_00950"
     CDS             complement(646..>1338)
                     /locus_tag="VNV63_00950"
                     /inference="COORDINATES: protein motif:HMM:NF015084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HXC61215.1"
                     /translation="PSTIRFYTWANPAVSIGAFQPLRDVNLDRCKQNDIRVVRRITGG
                     RALLHQKELTYSVICSIPSPFFPSNLQGCCRVIAEALQLGLQQLGLYVQIVSPESHRP
                     RSPHQADCFSSPSVYEITVDGRKLIGSAQRRWLRVFLQHGSLPIQTDRESEKELIIGT
                     IGNPSQRAVRLSELLNPLPSIKKLKDALTWGFEKKFGIILEEGALTPEEEKLAEELGQ
                     ARYAAETWTRRR"
BASE COUNT          318 a          313 c          359 g          348 t
ORIGIN      
        1 gtgtgtaatg atcttccgga agggaaggga agaactgaga cttcgagtct ttgccagagg
       61 cagaaggagc ggcatccaat gggtttaaaa caggagcagg cagtttttgt tcctgagatg
      121 cttgagctga ttttggaatg gttttagaat gccggatatt tgttgagtct ttcgcgtcga
      181 cagacgcccg tcctttgacc gggatggttc cgcaggctgt tatgaataat atggagatgg
      241 caacaaacgt tccgatcatt ttgaacggac ggagctgagc gatcaactgg gttcttttta
      301 tgtccatccc atccatctcc tgctgctctt caacgaataa atgatcggcg ggatgatacg
      361 ggttcttatc ctgcctgtca agtaaaatca gtttacttta tatgcatcta cgtttctaag
      421 tctaaccgat ttcttgtgat tgtcaactac acgaaagtgg gtattttagt tacatcacgt
      481 ctgtggtcta cctctacatg agtcttttaa ggataaattg acagagagac ttcaggcgac
      541 ctatccccga accagcagta gaggaagagc cgaaattaac catactgaag tataacgatt
      601 gcactaaacc aacttgatgt ctatagagaa tcagtagtct ggcggttatc tacggcgagt
      661 ccatgtttct gcggcgtaac gcgcctggcc aagttcctcg gccagctttt cttcctcggg
      721 agtcagagcg ccttcctcca agatgattcc aaatttcttc tcaaatcccc acgtcagcgc
      781 atccttcaat ttcttaatag aagggagcgg gttcagaagc tccgagagtc tgaccgctcg
      841 ctggctggga ttcccaatcg tgcctatgat gagttctttc tctgactccc gatcggtttg
      901 aatcggcaga gatccgtgct ggaggaaaac cctcaaccag cggcgttggg cactgccgat
      961 aagttttctg ccgtccacgg taatctcgta aaccgaaggg ctggagaagc agtcggcttg
     1021 gtgtgggctc cgaggacgat gagattccgg tgagacgatc tggacataca gtccaagttg
     1081 ttgtagcccg agctgaagcg cctcggcgat gacccggcaa cagccctgga gattcgaagg
     1141 gaaaaacggg cttgggatgg aacaaatcac actgtaggtc agttcctttt ggtgaagcag
     1201 tgcccgacct cccgtgatcc ttcggaccac tcggatgtca ttctgtttgc atcggtcgag
     1261 gttcacgtcg cgaagaggct ggaacgcccc gatcgatacg gcgggatttg cccaggtgta
     1321 gaagcggatc gtagatgg
//
LOCUS       DATMKH010000079         1061 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3563594, whole genome shotgun sequence.
ACCESSION   DATMKH010000079 DATMKH010000000
VERSION     DATMKH010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3563594"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(178..696)
                     /locus_tag="VNV63_00955"
     CDS             complement(178..696)
                     /locus_tag="VNV63_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HXC61216.1"
                     /translation="MSRPSKILVAGVGNIFMGDDAFGSEVARRLMSQGLPAEVQVTDF
                     GIRSYDLAYALTDGYDVTILVDVTTRKQSPGTVYLIEPDLSQLDQLDGMIADAHSLNP
                     VNVLQMLRSLGSAPGKLYLIGCEPAILDIEDGRIGLSEVVEAAIPQAIQLIKSLVNDL
                     LNETPKMKVETA"
     gene            complement(693..>1061)
                     /locus_tag="VNV63_00960"
     CDS             complement(693..>1061)
                     /locus_tag="VNV63_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61217.1"
                     /translation="DRTEALPEEDFWKMHGTIRGKKFSGEDFYNPNTRLDSVTVQGTD
                     LMVGHRVRIRPQGRADIMDLALEGKIAVIESIEQDAENRVHLALVLEDDPGKDLGMMR
                     QPGHRFFYGSHEVEPLIGNR"
BASE COUNT          214 a          302 c          269 g          276 t
ORIGIN      
        1 cttcctaagg ctcgattgca gtcgaccggt tggatgcatc ctcacatgtt actgatccgg
       61 agatatctct gcaggccggg cagactggcg atgatcacgg ccgtcatgat ggccgtcagg
      121 atgataagtg cggttgtgct cagttggcgc attcggttca cctccttccc aggtggttca
      181 ggcagtttct actttcattt ttggtgtctc gttcaaaagg tcgttgacca gggatttgat
      241 caattggatt gcctgtggaa tggcggcttc gacgacttca ctcagtccga ttcgtccatc
      301 ttcaatgtcc aggatcgcgg gttcacagcc gatcaaatac aacttacctg gagcgctgcc
      361 caacgatcgc agcatttgga ggacattgac cggattcagg ctatgcgcgt ccgcgatcat
      421 tccatccagc tgatcaagtt gactcagatc aggctcgatc aaataaactg ttcccggaga
      481 ctgctttcgt gttgtgacat cgactaaaat cgtgacgtca tatccgtccg tcagggcgta
      541 ggcgagatcg tagctccgaa tcccaaagtc cgtcacttgc acttcggctg gcaatccctg
      601 actcatcaat cggcgcgcta cttcgcttcc gaaagcgtcg tcccccataa agatgttgcc
      661 gaccccggcg accagaatct tgctgggtct gctcatcgat ttcctatcaa gggctcgact
      721 tcgtgtgagc catagaaaaa acggtgtccc ggctgtcgca tcattcccag gtcttttccg
      781 ggatcgtctt ccagcaccag ggccaggtga acccggttct ctgcatcctg ctcgatcgat
      841 tcgatcaccg cgatttttcc ttccagagca aggtccataa tgtcggcccg gccttgaggt
      901 ctgatccgga cacgatgtcc aaccatcaga tccgtccctt ggacggtcac gctgtccagg
      961 cgggtgttag ggttatagaa atcttcgcct gaaaatttct tgccgcgaat cgtgccgtgc
     1021 attttccaga aatcctcctc tggcaacgct tcggtgcgat c
//
LOCUS       DATMKH010000080         1162 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_679115,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000080 DATMKH010000000
VERSION     DATMKH010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_679115"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..>1162)
                     /locus_tag="VNV63_00965"
     CDS             complement(136..>1162)
                     /locus_tag="VNV63_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014274667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXC61218.1"
                     /translation="LDVFTFLKDVAKPSGGLQNAVTERGGISKLRCLAARKYPDVEIE
                     VHLADNGNEWSYAIGIKQEARGYRQPILAYERVTKKGRRLLERPDGDDLKDPLRLTQT
                     HLEQINANAEFRDLGRFFDSVSYLHLVPQLLRHPEAFSGPGIPGDPYGRGFLERVART
                     SEGTRKTRLRKIEAALRIAVPQLKELTDVKDDTGIPHLEVVYQHWRPQGAKQREDQFS
                     DGTLRLIGLLWSLLEGDSMLLLEEPELSLNSGIIRKLPALMYRIQRQKKRQIMISTHS
                     SDLLSDAGIGGEEVLLLTPSEEGTKVELASSIAEIRELLEGGLTVADAALPRTIPSNI
                     LQLDLFE"
BASE COUNT          260 a          331 c          286 g          285 t
ORIGIN      
        1 gcttcaggta tccgtagcca ccgcggttgt aaatcgtccg ggtggcgtat ccgactggaa
       61 tcgtcttaag aattttgagc gcaagggatt tggtcaatgc atcttcaaag gctaaattta
      121 ggggaattgg agtggtcatt cgaaaaggtc caactgaaga atattgctcg gtatcgtccg
      181 cggcagtgcc gcgtcagcga cagtcaggcc cccctctagc aactctctga tctccgcgat
      241 tgaggatgct aattccacct tcgtaccttc ctcacttggc gtcagtaata acacctcctc
      301 gcccccgatt cctgcgtcag aaagcaaatc cgagctatgg gtactgatca tgatctggcg
      361 ttttttctgc ctctgtatcc tgtacattag cgccggtagt ttccgaatga ttccggaatt
      421 cagagaaagc tcgggttcct ccagcagcag catcgaatcg ccctcaagca gagaccataa
      481 cagtccgatg agccgcagag ttccatctga gaattggtcc tctctctgtt ttgccccttg
      541 cgggcgccag tgctggtata ccacctccaa gtggggaata ccggtgtcgt ccttgacatc
      601 ggtgagttcc ttcaattgcg gaaccgcaat ccgcaaagcc gcctcaatct tccttaaccg
      661 cgtctttcgt gtgccttcgg aagtcctggc cacccgttca agaaaccccc gaccgtacgg
      721 atcgcccgga atacctggtc cagaaaaggc ctcggggtgc cgcaggagtt ggggcaccaa
      781 atggaggtag ctcaccgaat cgaaaaaccg acccaaatct ctgaattcag cgttcgcgtt
      841 tatttgttcg aggtgggttt gagtaagccg taaggggtcc ttcagatcat ctccgtcagg
      901 ccgctccaac agacgccgtc ccttctttgt gaccctctca taggccaaga taggctgccg
      961 gtaacctcga gcctcttgtt tgatcccaat ggcataagac cactcgttgc cattgtcagc
     1021 aagatgaact tcaatctcaa catctgggta ttttcttgca gcaaggcatc gcaacttcga
     1081 aatacccccg cgttcagtaa ccgcgttctg gagaccgccg ctaggcttcg caacatcttt
     1141 caggaaggtg aagacatcga gc
//
LOCUS       DATMKH010000081         2529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1640360, whole genome shotgun sequence.
ACCESSION   DATMKH010000081 DATMKH010000000
VERSION     DATMKH010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1640360"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="VNV63_00970"
     CDS             complement(<1..212)
                     /locus_tag="VNV63_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61219.1"
                     /translation="MANPAIKQSLDITIFGWLFLLSAPVELYSIISTGWKYTPKFFGI
                     TTTGLIAKIVLVAQPLLLVALGYGFLT"
     gene            complement(239..1111)
                     /gene="argB"
                     /locus_tag="VNV63_00975"
     CDS             complement(239..1111)
                     /gene="argB"
                     /locus_tag="VNV63_00975"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012676702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HXC61220.1"
                     /translation="MEKLIEKARTLTEALPYIRAFFGKTIVIKYGGKAMIEEELKEQF
                     AEDIALMKYVGMKPVVVHGGGPQINQMLKRLGREPKFVRGVRVTDRETMEVVEMVLGG
                     TINKEIVARINRHGGKAVGLSGKDGRLITARPLHTKPFRPGEKRLDFGQVGEVEAIDT
                     QVLNSLDQDRFIPVIAPIGVDSAGKTYNINADLVAGAMAAALKAEKLIVLTDVKGILD
                     QKKKLIPTLPKKQMMRFIKSGVISEGMLPKVESCLTALEGGVRKAHIINGCVPHALLL
                     EIFTDQGVGTEIVV"
     gene            complement(1128..1994)
                     /locus_tag="VNV63_00980"
     CDS             complement(1128..1994)
                     /locus_tag="VNV63_00980"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXC61221.1"
                     /translation="MTQPLEEKVLKSFDGTEIYYLTGGSKDPKAHTLLLIHGLGSNAT
                     RWTKLAQLPFCQEQCRLIILHLRGHGHSSTRRNVGIDSFAKDCLAVLDQEKCEKAILI
                     GHCLGANIAGRCWELNKTHIAGIVLIEPFFAKSLRRDWKFFNFFLTPILLLVAGTAKL
                     LNAIGIRRRNFPMIDYYTYDEWVRTRLHTSIDVIRWMGPWADLHTMPVTSYIASYRTL
                     YTYAPDWKKVTAPMLVIFAKGEQMFSGERDGHLFGDNPKVMVRTIEGSHFMLTDNVQE
                     VAQAIQEFVKTI"
     gene            complement(2061..>2529)
                     /locus_tag="VNV63_00985"
     CDS             complement(2061..>2529)
                     /locus_tag="VNV63_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXC61222.1"
                     /translation="TVNTKYGMVRTDHILFIAAGAFHVSKPSDLIPELQGRFPIRVEL
                     EPLVKEDFIRILTEPKNALIKQYIGLLQTEGLKIEFTQDAIEEIASLAVLVNERTENI
                     GARRLFTIMEKLLEELSFEAPGLAVKEHKIDAKYVKERLSEIVKSEDLSRYIL"
BASE COUNT          565 a          727 c          618 g          619 t
ORIGIN      
        1 gtcaagaaac catagcccaa agcaacaaga agaagcggct gcgccacgag aacaatcttg
       61 gcaatcaagc cggtcgtggt gataccgaaa aatttcggcg tatacttcca tccggttgag
      121 atgatgctgt aaagctcaac cggcgcgctc aataaaaaaa gccagccgaa gattgtgatg
      181 tccagagact gtttgattgc cggattcgcc atgcgaatcg ccgttgtttc tagaagggtc
      241 aaaccacaat ctcggtgccg acaccctgat ccgtgaagat ctcaagcagg agggcatggg
      301 gcacacaacc attaatgata tgtgccttac gcacgcctcc ttcaagcgcg gtcaagcagg
      361 actcaacctt gggcagcatg ccttccgaga tcacgccgga tttgataaag cgcatcattt
      421 gcttcttggg aagcgttggg atcagtttct tcttctgatc caggatgccc ttcacgtcgg
      481 ttaaaacgat gagcttctcc gctttgagcg ccgccgccat cgcgcccgct accagatcgg
      541 cattgatgtt gtatgtcttg ccggcgctgt ccacgccgat cggcgcgatc accgggatga
      601 agcggtcttg atctagactg ttcaaaacct gcgtatcaat cgcttccacc tctccgacct
      661 gaccaaaatc caaccgtttc tctccggggc ggaacggttt cgtatgaaga ggtcgagccg
      721 taatcagccg tccatctttg cctgaaagcc cgaccgcttt gcctccatgg cgattgatcc
      781 gggccacaat ttctttattg atcgttccgc ccaggaccat ttcgaccact tccatcgttt
      841 cgcggtccgt gacgcgaaca ccgcgcacaa acttgggctc tcgtcccagc cgcttcagca
      901 tctggttgat ctgcggcccg ccgccatgaa ccaccaccgg tttcatgcca acgtatttca
      961 tcagggcgat atcctcggcg aactgttctt ttaactcctc ttcgatcatc gctttgccgc
     1021 cgtatttgat gacaatcgtc tttccaaaga aagcgcggat atatggcagc gcttcggtca
     1081 gcgttcgggc cttttcaatc aacttttcca tggacacgct tttctctcta aatcgtcttc
     1141 acaaactcct ggattgcctg ggccacctcc tggacattgt cggtcagcat aaaatggctt
     1201 ccttcaatgg tgcggaccat gaccttcggg ttgtcgccga agagatggcc gtccctctcg
     1261 ccggagaaca tctgttcccc cttggcaaag attaccaaca tgggcgcagt cacctttttc
     1321 caatccgggg cataggtgta gagcgtccga taggaggcga tatagctcgt gaccggcatc
     1381 gtatgcaggt ccgcccaggg tcccatccat cgaatcacat caattgaagt atgaaggcgg
     1441 gtcctcaccc attcatcata ggtgtagtaa tcaatcatgg gaaaattcct tcgccggatg
     1501 ccgatcgcgt tgaggagctt cgccgtcccg gctaccagga gaagaatcgg ggtcaggaaa
     1561 aaattaaaga acttccagtc ccggcgaaga cttttggcaa aaaacggctc gatcagcaca
     1621 atcccggcga tgtgcgtctt atttaactcc caacaccgac cggcgatatt cgctcccaga
     1681 cagtggccga tcagtatcgc tttctcgcat ttttcttgat ccaggacggc caagcagtcc
     1741 ttcgcaaaac tgtctatacc gacatttctc ctggtgcttg aatgtccgtg ccctctgaga
     1801 tgcaatatga tcaaccggca ttgctcctgg caaaaaggaa gttgcgcgag cttggtccag
     1861 cgggtcgcat tgctcccgag gccgtgtatc agaagcagtg tgtgtgcctt cggatctttt
     1921 gagcctccgg tgagataata gatctccgtc ccgtcaaagc tcttcagcac cttctcttcc
     1981 agcggctggg tcatcaactc atggctcgaa ttagtgacgt ctcgttcagg acaggtcggc
     2041 gacccgcccc tacaaaaatc ttacaaaatg taccggctca agtcctcgct cttgacgatt
     2101 tcgctcaagc gttccttgac atactttgca tcaatcttgt gttccttgac cgccagtccc
     2161 ggcgcctcga aggagagttc ttcaaggagc ttctccatga tcgtgaacag cctcctggcc
     2221 ccgatgttct cggttctttc gttgactagg accgcaagcg atgctatctc ctcgatcgca
     2281 tcctgagtaa attcaatctt cagaccctcg gtctggagca gaccgatgta ttgcttaatc
     2341 aaggcgttct tcggctcggt cagaatccgg ataaaatctt ccttgaccaa gggctccagc
     2401 tctacacgga tcggaaaccg gccctggagt tccgggatca gatcagaggg cttagagaca
     2461 tggaacgccc cggccgcgat aaacaagata tggtcggtgc ggaccatccc gtatttggtg
     2521 ttgactgta
//
LOCUS       DATMKH010000082         1136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4210188, whole genome shotgun sequence.
ACCESSION   DATMKH010000082 DATMKH010000000
VERSION     DATMKH010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4210188"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="VNV63_00990"
     CDS             complement(<1..475)
                     /locus_tag="VNV63_00990"
                     /inference="COORDINATES: protein motif:HMM:NF017936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXC61223.1"
                     /translation="MKSYFLSLLIALLAVTAQAQRGNKKPTAEEVIEKLELKPLPDEG
                     GYYRQTYKSDVGLPANIVGIRSDLMRPISTAIYFLETPDGFSALHRIKGDEVYHFYGG
                     DPVEMIQIDSSGALSRFVLGSDILNNQSPQVVAPKGIWQASKLKAGGRWALMGTTV"
     gene            complement(753..>1136)
                     /locus_tag="VNV63_00995"
     CDS             complement(753..>1136)
                     /locus_tag="VNV63_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61224.1"
                     /translation="ERIVPPEGLLIPEDTRISIYENVILQLIKTKKFQHVYRSGDRTA
                     ADAPDLVILHLIPEGFKPGSQKEREVITIAGATSIKVKVQLTSREGKVLLEKDVEGKV
                     RFYGENLRATYDLAKKVAGVVKGSF"
BASE COUNT          253 a          315 c          285 g          283 t
ORIGIN      
        1 cgactgtcgt acccatcaac gcccatcggc caccagcctt taacttagac gcctgccata
       61 tcccttttgg tgctacgacc tgaggagatt ggttgttcaa aatgtcggaa ccaagtacga
      121 accgagacag cgccccggaa ctgtcaattt gaatcatctc cactgggtcc ccgccataaa
      181 aatgataaac ctcatcaccc ttgatccgat gaagggccga aaagccgtcc ggcgtttcta
      241 gaaaatagat ggccgtgctg atgggcctca tcaaatccga gcgaataccg acgatgttcg
      301 ctggtagtcc tacgtcgctc ttgtaagtct gtcggtaata tccgccctcg tcaggaagcg
      361 gcttgagctc caatttttca atgacttctt ctgctgtcgg ctttttattc cctctttgtg
      421 cctgtgcagt cacggccaaa agggcgatca gtagagaaag aaaataactc ttcatgcggc
      481 ttagtccctt acttcctcgt cttcggatac tctcggtgag gccacgcaga acctcttgga
      541 gttggccgac aaccaagcag acttcgttcg taattgtaac tgattgtgtc cattgcccgc
      601 gaaacgggat tatcggcatg tagcccacac caggaccgac aagaaagttg gtcggtttcc
      661 gagaggcaag tgactgggct atggcgacac ccggacacag agcttcaatt cgcattgtcg
      721 gatgggttct gcggcagcta ttaatccacc atttagaatg atcccttgac tacaccagcg
      781 actttcttgg cgaggtcata cgtggctcgc aggttttcgc cgtagaagcg caccttgcct
      841 tcaacatcct tctcgaggag gaccttgcct tcgcgcgaag tcaattgaac cttcaccttg
      901 atggaagtgg ctcccgcgat ggtgatcact tcccgctctt tctggctgcc tggcttgaaa
      961 ccctcgggga tcagatgaag gatcacgaga tcgggcgcat cggctgccgt gcggtcgccg
     1021 ctccgataga cgtgctgaaa cttctttgtc ttgatcaact ggaggatcac gttttcgtat
     1081 atcgaaatgc gggtatcctc cgggatcagc aaaccctcgg gcgggacaat gcgttc
//
LOCUS       DATMKH010000083         1096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_362139,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000083 DATMKH010000000
VERSION     DATMKH010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362139"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..651
                     /locus_tag="VNV63_01000"
     CDS             118..651
                     /locus_tag="VNV63_01000"
                     /inference="COORDINATES: protein motif:HMM:NF014417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HXC61225.1"
                     /translation="MTVAPNLSVPLSPQEAHWYSIQTRYRFERKVTIQLQRKGLQTFL
                     PLLEEFHRWSDRRQPIYIPLFSGYTFAYVDLSAGLRIELLHTEGVIGLISVTGVATPI
                     PVKQIDDLRSLLSQKVPCALHAFLTVGQKVRIRGGCLDGLEGILEQRGEKNLVISIES
                     IQRAVAITIEGYELEMI"
     gene            653..>1096
                     /locus_tag="VNV63_01005"
     CDS             653..>1096
                     /locus_tag="VNV63_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61226.1"
                     /translation="MRSPLTIKPVTPIRKFASPSRLIPGVQASVDGARRKKSSSAIVD
                     GRFWEWQDHDGLGEPRKRRLSEVLAEALFPRLSVHGQRWNLARQVSADFLLIIFGFAA
                     VGHLIVLLEFAIHHDPAALLRPEPFPTAGPGLLLLYGALFTLLGFS"
BASE COUNT          247 a          313 c          280 g          256 t
ORIGIN      
        1 aaccgaagca gtgtgaaaca agaattctga acggaaggaa cttagaaacg tcgtgaaaaa
       61 tagagtaaaa atcattcggg acccaattag cggagccgcg ttccaagaat cgtcttgatg
      121 accgttgcac ccaacctttc cgtaccactt agcccgcaag aagcacactg gtactcgatt
      181 cagacccggt accgctttga aagaaaagta accattcaac tccaacgcaa aggcctgcag
      241 acattccttc cgttgctcga agaatttcac cgctggagcg atcgccgcca acccatctat
      301 attcctcttt tctcgggcta tacgttcgcg tatgttgact tgtccgccgg attgcgaatt
      361 gaactcttgc acaccgaagg cgtcatcggc ttgatcagcg tcacaggagt agcaactccg
      421 attcctgtca agcaaatcga tgatctccga agcctcttat cccagaaagt cccatgcgcc
      481 ctccacgcgt tcctgaccgt cggacaaaag gtaaggattc gaggtggttg cctggacggc
      541 ctggagggaa ttctggaaca gcgtggcgag aagaatctgg tgatctcgat cgaatccatc
      601 cagcgcgcag tcgccatcac tatagaagga tacgaactgg aaatgatctg aagtgcgttc
      661 ccctctgaca atcaagcctg tcaccccgat ccgcaagttt gcgtctccct cccgtttgat
      721 tcccggagtg caagcatcgg tcgacggagc tcgtaggaag aaatcctcct cggccatcgt
      781 ggatgggcga ttctgggaat ggcaggatca cgacggtctg ggtgaaccga gaaagcgccg
      841 tctctccgag gtcctcgcgg aggctctctt cccgagactt tcagttcacg ggcagcgctg
      901 gaatcttgcc cggcaagtca gcgcggactt tctcctgatc atcttcggct ttgccgcagt
      961 tggccatttg atcgtgcttt tggagttcgc gatccaccac gatcccgcgg cgttactgcg
     1021 tcccgaaccg tttcccaccg ctgggccggg attgctgttg ctctatggag ctctgtttac
     1081 cttgctgggt ttttcc
//
LOCUS       DATMKH010000084         1112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2928415, whole genome shotgun sequence.
ACCESSION   DATMKH010000084 DATMKH010000000
VERSION     DATMKH010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2928415"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            299..>1112
                     /locus_tag="VNV63_01010"
     CDS             299..>1112
                     /locus_tag="VNV63_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXC61227.1"
                     /translation="MRSASCLAILSVVMIVFGACPGVSAQGVATIQVETHLIDTAVSV
                     HDGDGRVVSGLSQEDFTVVEDGVPQKIRFFAHDNELPLSIGLIIDESGSQEKFVKEHE
                     KDIEAFLRQVLEPNDQAFAVCFGNHLRLVSDSTSSTSAIMDGIRSFDQGNTDFPEIGP
                     KEQRELGTALYDAIYFGITEKLANIHKRRKVLVVFSDGEENSSEHDLLDAVEMAQNSD
                     VMVYAIRYTDMKHGKMDARDRYGMRALDHVTGQTGGKTFDFHATNLKKAFEEI"
BASE COUNT          226 a          239 c          368 g          279 t
ORIGIN      
        1 tgcggtagtt ttcttctctt tattctggtg tggtggaacc acgttttttc ggggggtttt
       61 tgagaaaacg ggtgttttgt gcgtggtttt ttggtggtga aaacgtggtg gaacgtgtaa
      121 cgaacgtggt tcttagaagc acactttttc tcgccgaaag tctgccactt tttgaaaata
      181 tttctgtgga aaatccggtt tcgggatagg gtgtttttgg ggcgatgagg aaccggtagc
      241 cgggattgat cgtaccgcct tctgagaccc atttctatcg cccgttccag gagcccaggt
      301 gcgttctgca agctgtcttg cgatcctctc tgtcgtcatg attgtgtttg gggcctgccc
      361 gggcgtttcc gcgcagggtg tcgcgacgat tcaggtggag acgcacctga tcgataccgc
      421 ggtcagcgtc catgatgggg acgggcgggt tgtctctggg ctgagccagg aggatttcac
      481 ggttgtcgaa gacggggttc ctcagaagat ccggtttttt gcccatgata acgagcttcc
      541 tttgagcatc gggctgatca tcgatgagag cgggagccag gagaagttcg tcaaggaaca
      601 tgagaaggac attgaagcgt tccttcggca ggtgctggag ccgaacgatc aggcgtttgc
      661 tgtctgcttc gggaatcatc ttcggctggt cagcgactcc acctcatcga cgagcgcgat
      721 tatggacggg attcgcagct tcgatcaggg caacaccgat tttcccgaga tcgggccgaa
      781 ggagcagcgg gagctgggga ctgcgctcta cgatgcgatc tatttcggga ttacggagaa
      841 gctggcgaac atacacaaga gacgaaaggt gctggttgtc ttcagcgacg gggaggagaa
      901 ctcgagcgag catgatttgc tggacgcggt cgagatggcc cagaactccg atgtgatggt
      961 gtatgcgatt cggtacaccg atatgaagca cggcaagatg gatgcgcggg atcgttatgg
     1021 gatgagggct ctcgaccatg taacggggca gacgggcggg aagacgttcg actttcatgc
     1081 gacgaatttg aagaaggcgt ttgaggagat tg
//
LOCUS       DATMKH010000085         1084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1964524, whole genome shotgun sequence.
ACCESSION   DATMKH010000085 DATMKH010000000
VERSION     DATMKH010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1964524"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VNV63_01015"
     CDS             <1..225
                     /locus_tag="VNV63_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61228.1"
                     /translation="TVISTEAAHGLIVSSAAEKSASLPRQHLSHRRVSAVVLGLGNLT
                     NQVLPDPQKINSSLTARLKYPRKYRPNPSS"
     gene            complement(286..561)
                     /locus_tag="VNV63_01020"
     CDS             complement(286..561)
                     /locus_tag="VNV63_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF507 family protein"
                     /protein_id="HXC61229.1"
                     /translation="MRISRDKLNKLAHTVADTLAEIAEVDFLEDRNTIRQEARKALEK
                     LLMEETKIDAAARMKISSQRKIITEGSQEWDILYRKYYNDEVKKLGL"
     gene            complement(558..836)
                     /locus_tag="VNV63_01025"
     CDS             complement(558..836)
                     /locus_tag="VNV63_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013580023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF507 family protein"
                     /protein_id="HXC61230.1"
                     /translation="MIFSKDYVGYLARQTVKHLVAEKMIHTEKTALVDERVGAAMIDE
                     LSLEDRINDEVRVILEAFQDDMRKTGASYPEMFKKVKNELARKYKAVL"
     gene            complement(866..>1084)
                     /locus_tag="VNV63_01030"
     CDS             complement(866..>1084)
                     /locus_tag="VNV63_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013568407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HXC61231.1"
                     /translation="RVNETSCLDQCEHGPTVVVYPDAVWYGFVDAGDVEEIVNQHLLH
                     GRPVDRLRLADTCLNTERCPHKPRSKEE"
BASE COUNT          236 a          359 c          233 g          256 t
ORIGIN      
        1 accgtcattt cgaccgaagc tgctcacggt ctcatcgtga gcagcgcagc ggagaaatcc
       61 gcttctctac ccagacagca tctcagccac cgccgcgtct ctgccgttgt cttagggttg
      121 ggaaacttga caaaccaggt cttgccagac ccccaaaaaa tcaattccag tctcacggca
      181 cggctaaaat acccaaggaa gtacagacct aacccgtctt cttagaagac tttgcacact
      241 ttttggggga aggggggtac taacgccaat gcggtaagaa gcctctcaca gcccgagctt
      301 ctttacctca tcgttatagt atttccggta aagaatgtcc cattcctgcg agccctcggt
      361 aatgatcttc cgctgcgaag agatcttcat ccgcgccgcc gcatcgatct tcgtctcttc
      421 catcagcagc ttctcgagag ccttccgcgc ctcctgccga atcgtattcc ggtcctccag
      481 aaagtccacc tcggcaatct ccgccagagt atccgcaacc gtatgggcca gcttgttcaa
      541 cttgtcgcgc gaaattctca cagcaccgcc ttgtacttgc gggcaagttc gttcttcacc
      601 tttttgaaca tctcagggta gctcgcgccg gtcttgcgca tatcatcctg aaacgcctca
      661 aggataaccc ggacctcatc attgatccgg tcttcgagcg acagctcatc gatcatggcc
      721 gccccgacgc gctcgtccac caaagccgtt ttttcggtgt ggatcatctt ctcggcgacc
      781 agatgtttca ccgtctggcg tgccaggtat ccaacataat ctttagaaaa aatcatctgt
      841 ctttccttcg actataccac gcacatcatt cttccttcga cctcggctta tgcggacaac
      901 gctctgtatt caggcacgta tcggccagcc gcaaccgatc taccggccgc ccgtggagca
      961 gatgttggtt cacgatctcc tccacatccc ccgcatcgac aaacccatac caaaccgcat
     1021 ccggatacac caccaccgtc ggtccatgct cgcactggtc caggcacgag gtctcattca
     1081 cccg
//
LOCUS       DATMKH010000086         1148 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1643198, whole genome shotgun sequence.
ACCESSION   DATMKH010000086 DATMKH010000000
VERSION     DATMKH010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1643198"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            452..1111
                     /locus_tag="VNV63_01035"
     CDS             452..1111
                     /locus_tag="VNV63_01035"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HXC61232.1"
                     /translation="MRVGWNWRYAQHCLICKKWLSKSSKLLILTLLLSILVFAFPTPS
                     AYVFSEENPGLIEEAGLQIADASLDPAIGSMDVFLSRYKVDEAHRGRIAESIVSSSRK
                     YNIDPHLVASIMIVESRANPFAISNSDSIGIMQIHLPTWGPTADQEGINLFKIEDNVD
                     FGVRILKDYVRKFGLWEGVKRYKGWYPDSTDSAQAVGDYLSKVQRIYSAAGLQPTEVL
                     Q"
BASE COUNT          272 a          300 c          297 g          279 t
ORIGIN      
        1 acggcggcct gaaccagtcc ctggtagtag agccggagcg ggcctcttgc cggacgccag
       61 agatcctccc aggcttcgtg agcttcaaag tagcgcccgg cattgaaaaa aaatattccg
      121 tcctggagga ggttgggcaa cggtgaaatc ctctaagtta ttgagagatt tgccacagaa
      181 cgctaggaaa gggtgtagta aaacccccta tcctagccca gttgggctat cacctcaagg
      241 ggtttctggg gtgccaggga gacggccgct tctttccttg gcaaaggcac cccggctccg
      301 tatcattttc gccaggcgct gctgaaagac ggtagctctg ggatctggaa tatccattcc
      361 agttggtcgt cattagaaat aacaaggact agcccttccc cccattagga ggatcatgca
      421 acccattgca ttttgcagtc gatgtcgagg catgagagta ggctggaatt ggcgttacgc
      481 acagcattgc cttatctgta agaaatggct ttcgaaatcc tctaagttgt tgattcttac
      541 gctacttttg tcgattcttg tttttgcatt cccgactccg agcgcttacg ttttttccga
      601 ggaaaatccc ggattgatcg aagaagccgg cctgcagatc gccgatgcct ccctggatcc
      661 ggccataggc tcgatggatg tgtttctcag cagatataag gtcgatgagg ctcatcgcgg
      721 ccgaatcgcc gagtccattg tgagcagctc ccgcaagtac aacatcgatc cccatctggt
      781 tgccagcatc atgattgtcg agagccgggc caatcccttc gctatctcca acagcgattc
      841 catcggtatc atgcagatcc accttccgac gtggggtccg actgccgatc aggagggaat
      901 caacctcttt aagatcgagg acaacgtcga ttttggcgtc cggatcctta aagactatgt
      961 ccgtaagttc ggcttgtggg aaggcgtcaa gcgatacaaa ggctggtatc cggatagtac
     1021 cgattctgcg caagccgtag gcgattatct ttcgaaagtt cagcggatat actccgccgc
     1081 cggcttgcaa cctaccgaag ttcttcaata gtttacaatt tcgaagggaa taaccacaaa
     1141 gacacaaa
//
LOCUS       DATMKH010000087         1334 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2929877, whole genome shotgun sequence.
ACCESSION   DATMKH010000087 DATMKH010000000
VERSION     DATMKH010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2929877"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1020)
                     /locus_tag="VNV63_01040"
     CDS             complement(<1..1020)
                     /locus_tag="VNV63_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HXC61233.1"
                     /translation="MAVSELPFRQVHLDFHTSEKIQGVGSNFDPDEFAETLVKARVNS
                     ITCFARCHHGWLYFESKKFPEREHPALRRDLLKQQIEACHRRGIRMPIYITVQWDYYT
                     ATRHPEWVCMDKNGSLVGTPPFEAGFYQQLCLNTPYREFLKELTVEVLETFPTDGLFF
                     DIVWPVACVCRYCREKMSAKGLEPGDAGVSERFGIETVNQFKEEMTQFIRDRNSHCTI
                     FYNAGHIGPRHRQVKHAYSHFEIESLPGGEWGYLHFPATIRYARTLGLECVGMTGKFH
                     TSWGDFHSFKNLQALRYECLRMLTHGAKCMIGDQLHPDGRIDPHVYALIGQVYQEIEQ
                     KEPWCI"
     gene            complement(1037..>1334)
                     /locus_tag="VNV63_01045"
     CDS             complement(1037..>1334)
                     /locus_tag="VNV63_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61234.1"
                     /translation="NDMKVFVPFAMILLADFEGEQKHSAEAIHYAEQAIQLAKPGNSP
                     RALYSAHLTLAKLYFEKGKTTEARQLAEQSRAEAEAFGSGEDAKEAEDFLQKHP"
BASE COUNT          300 a          334 c          353 g          347 t
ORIGIN      
        1 gatacaccaa ggctccttct gttcgatttc ttgataaact tgaccgatta atgcgtagac
       61 atgcggatcg atccggccgt ctgggtgcaa ctggtcgccg atcatacact tggcgccatg
      121 ggtcaacatc cgcagacatt catagcgaag cgcctgcagg ttcttgaatg aatggaaatc
      181 tccccatgac gtgtggaatt ttccggtcat tccaacacat tctaatccca gggtccgggc
      241 gtatcggatg gtcgccggga aatggaggta tccccattcg ccgccaggga ggctttctat
      301 ttcgaagtgg gagtaagcat gcttgacctg gcgatggcgg gggccgatat gccctgcgtt
      361 atagaagatg gtgcaatgag agttccggtc tcggatgaac tgggtcatct cttctttgaa
      421 ttgattaacc gtttcaattc cgaagcgttc tgacactcca gcgtcgccag gctctaagcc
      481 tttagctgac attttttcgc ggcagtagcg acatacgcaa gcgaccggcc agacaatgtc
      541 aaagaacagc ccatcggttg ggaacgtttc caggacctcc actgtcagct ctttcagaaa
      601 ttctcggtag ggtgtgttta gacaaagttg ttggtaaaac ccagcctcga acggaggggt
      661 accaactaaa gagccgttct tatccatgca cacccattcc gggtgtcgcg ttgcggtata
      721 ataatcccat tggacggtga tataaatcgg catccggatg ccgcgacggt ggcatgcctc
      781 gatctgttgc ttgagcaggt ctcttctgag ggcaggatgt tcccgttcag ggaatttttt
      841 ggactcgaag taaagccacc cgtggtggca acgcgcaaag caagtgatcg agttcaccct
      901 ggctttgacc aaggtttccg cgaattcgtc cggatcgaaa ttggatccga caccttggat
      961 cttttcgctg gtatgaaagt cgagatggac ctggcggaac ggtaattcag agactgccat
     1021 tctatgaagg atgtcgttat ggatgtttct ggagaaaatc ttcggcttcc tttgcgtcct
     1081 caccactgcc gaaggcttca gcctcagctc ggctttgctc tgccagctgt ctggcttccg
     1141 tcgtcttccc tttttcgaag tatagcttag ccaaggtcaa gtgagctgaa taaagggcac
     1201 gaggactatt cccaggcttt gccaattgga tagcctgctc cgcataatgg attgcttcag
     1261 cggaatgctt ttgttcacct tcgaaatcgg ccaagaggat catggcgaac ggtacgaaga
     1321 ccttcatatc attc
//
LOCUS       DATMKH010000088         2234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1644153, whole genome shotgun sequence.
ACCESSION   DATMKH010000088 DATMKH010000000
VERSION     DATMKH010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1644153"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..>2234)
                     /locus_tag="VNV63_01050"
     CDS             complement(8..>2234)
                     /locus_tag="VNV63_01050"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF004349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HXC61235.1"
                     /translation="SDIEVEHEDVNGKMYYIKYPLADDPETFLTVATTRPETMLGDTA
                     VAVHPDDPRYNTLIGKKILLPLTNRHIPIVGDPILVDREFGTGAVKITPGHDFNDEKA
                     GQRHGLRAISILNEKGQIQPFLEQEGVDKNVLNDLMLLDVPKARARVEFWLGERGYLL
                     KVEDHKHAIGKCYRCKTVVEPYRSPQWFVKVNDPKNSLAAPAIEAVRSGRIRMIPEGW
                     KNNYFGWMENIQDWCISRQIWWGHQIPAWYCKKCNQDSLLHTRPDGSPIIGKNAVVIV
                     SRTEPTECSRCKGRDLVRDPDVLDTWFSSALWPFSTLGWPEKTKPLSTFYPTSVLVTS
                     FDILFFWVARMIMMGLKFMNEVPFKDVYIHALVRDAEGQKMSKSKGNVIDPLEIMEKH
                     GTDALRFTLASMASQGRDIKLSEERIEGYRNFANKIWNAARFILMNCPQGAKAYLEAP
                     LPNPKTLSLADRWILSRLQKIIASVNQQLEDYRFDEASRELYQFLWHEFCDWYLELIK
                     PTLYGGDTNAADQSRTVAIHVFEAVLRLLHPFMPFITEELWQIIHQPTDKSIVAAPYP
                     TSSNRSLEDEALEGIMNNIISIATSIRTIRNEHNIPPSQQLKAGIVTLNDDYAKTILR
                     DGEADIRRLAKLSEFTAGPSIVIPSGALEISTSAGKVFIIVDVDPLKEKARLEKKLAA
                     KKSEFDREDKKWNDPGFQKNAPAEVKTENDLRRNKLSKEVLELSENLRKITGALKEHP
                     S"
BASE COUNT          493 a          546 c          592 g          603 t
ORIGIN      
        1 agtgtaatca tgaggggtgt tccttaaggg cgcctgtaat cttacgcaga ttctctgaga
       61 gctcgagcac ttccttgctc agcttattgc ggcgcagatc attttcggtt tttacttcgg
      121 cgggtgcatt cttttgaaac cctgggtcat tccatttttt atcttcccga tcaaattcag
      181 acttcttcgc tgcgagcttt ttctctagtc gagctttctc cttgagcggg tctacatcca
      241 ctatgataaa aactttccct gcggatgtag agatctccaa ggctcccgat ggtataacga
      301 tagatgggcc ggctgtaaac tctgacagct tggcaagtct tcgaatatca gcctctccat
      361 cgcgtaaaat ggtttttgcg tagtcgtcat ttagtgtgac tatgccggct ttaagctgtt
      421 gcgacggcgg gatgttatgt tcatttctga tggtgcggat ggaggtagca atactgataa
      481 tattattcat tatgccttct aaagcttcat cttcaaggga acgattgctg gaagtcgggt
      541 agggagcagc aacgatgctc ttgtcggtcg gttggtgaat gatctgccag agctcttccg
      601 taatgaacgg catgaacgga tgaaggaggc gcagaacggc ttcaaaaaca tgaatcgcaa
      661 cggtccggct ttgatctgca gcatttgtgt ctcctccgta caaggtcggt ttaatcagtt
      721 caagatacca gtcacagaac tcatgccaga ggaattgata aagttcgcgc gaggcttcgt
      781 caaaacggta atcctcaagt tgctggttca ccgatgcgat gattttctgt aggcgactta
      841 agatccaacg atcggcgagt gagagcgttt taggatttgg aagcggtgct tctaagtagg
      901 ccttcgcacc ctgcggacag ttcatcaaga taaaccgtgc ggcgttccat attttatttg
      961 cgaagttccg atagccttcg atccgttcct cggagagttt gatatcccga ccctgcgaag
     1021 ccatcgaggc aagcgtaaat cgcagggcgt ctgttccgtg cttctccatg atttcgagcg
     1081 ggtcgatgac gttgcctttg gacttgctca tcttctgacc ttctgcatca cggaccaggg
     1141 catgaatata gacgtctttg aacgggacct cgttcatgaa tttaagcccc atcatgatca
     1201 tacgggcgac ccagaaaaag aggatgtcga agcttgtgac caggaccgaa gtagggtaga
     1261 aggtcgaaag cggcttagtc ttttccggcc agcccaaggt cgagaagggc cagagagcgg
     1321 atgaaaacca ggtatctaat acatcggggt cacggaccag atcgcgacct ttgcacctgg
     1381 aacattccgt gggttccgtc cggctgacga tgacgactgc atttttgcca ataataggcg
     1441 aaccatccgg gcgggtgtgc agtagcgaat cctggttgca cttcttgcag taccaggctg
     1501 gaatttgatg gccccaccag atctgccgtg agatgcacca gtcctgtata ttctccatcc
     1561 agccgaagta gttgttcttc caaccttccg ggatcatgcg gattcggccg gatcggacag
     1621 cctcgatcgc gggcgcggcc agtgaatttt tcggatcgtt gactttgacg aaccattgag
     1681 gtgagcggta cggctcgacc acggtcttgc agcggtagca tttgccgatg gcatgtttgt
     1741 ggtcttcgac cttcagcagg tagccgcgtt cacccaacca gaactctacg cgagctcttg
     1801 cttttggaac atccagcagc atgaggtcgt tcaggacatt tttgtccacg ccttcctgct
     1861 cgagaaacgg ttgaatctgt cctttttcat tcaaaatcga gattgcgcgt aaaccatgac
     1921 gttgacctgc tttttcatcg ttaaagtcat gccccggtgt gatcttcacg gcgccggttc
     1981 cgaattcacg atctactagg atcggatcgc ccacgatcgg aatatggcgg ttggtcaacg
     2041 gcagaaggat ctttttccca atcagcgtat tgtatcgagg gtcatccgga tggacggcca
     2101 cagcggtgtc tcccagcatt gtctcggggc gggttgtggc gacggtcagg aaggtttcgg
     2161 ggtcatcagc caaaggatat ttaatgtagt acatctttcc gttgacgtcc tcgtgctcca
     2221 cctcaatatc cgag
//
LOCUS       DATMKH010000089         3045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_683727,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000089 DATMKH010000000
VERSION     DATMKH010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_683727"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="VNV63_01055"
     CDS             <1..480
                     /locus_tag="VNV63_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61236.1"
                     /translation="NWWLAIYGYYIPANVRIGLSVPQVLGTLTCLFAGCGINIAMMKG
                     SKPTGPVEWGKISVRSQYALFALGVSFTWLMALMGYIRSSVRLFWHVMEVFRDSSPWA
                     FTHTIGFAANVMSFNVLVFWVFCLFVFWLGELTTAHAVKEAKRGKVIGETVIAGGSE"
     gene            578..1816
                     /locus_tag="VNV63_01060"
     CDS             578..1816
                     /locus_tag="VNV63_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase"
                     /protein_id="HXC61237.1"
                     /translation="MTQTLFATSISLLALFFLLLGYFVPRAKVLMFSIGFYFVMTALF
                     AGYSNWLPQVRGEVPQETKIDPGDILNMPKDKLVDMGETIIFGGVGGMDSRRTDGKGQ
                     CPLCHTFKKGDIGDRAPNLLGIGTRAAQRIKDPRYLKPNTVQTEAFPGSGRATTAEEY
                     IAESHSCPSCFVVEGFGVKGTNDRESPMPTIHKPPVSLSIPELIAVDTFLWARLSPEE
                     ATPSPAEIKAAYEKFIPPAERQQGAPTAVTTSAPAPAGPPIALGTDTPEQIITKMGCF
                     ACHQIPTIAAAKFGPIGPLLVEKTNAPKRIASPEYQAMLKAGKAHARTPKEYVMESIV
                     TPNAFIVHAFVQKANPDTSDMIQDFSQKFTFGALEKLADFLLTLDCDAAKKDGLKGPP
                     AEPIDQVCGKGPEKTASLAQ"
     gene            1871..2797
                     /locus_tag="VNV63_01065"
     CDS             1871..2797
                     /locus_tag="VNV63_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009383529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HXC61238.1"
                     /translation="MKIWIPGYMPIQLLLIFGLFLVLSLGVGTLYRRITGRPLHMLIQ
                     GTGAWLIAYFVLRFVLYPPIPLSVFSTYMGLITLVIFLYMSSTEAGWQETQKPILDTL
                     KGETQIYRGVRAVTFVALPILIGYGTFSQVVPKVDEPIELRTVHPAPPQNTTVHGNLF
                     TLQTAQNPYRVDDEGKYLPVGKEDKYFNKNAFDPNAPKFLQYVKEGGQVFFNTAGCFM
                     CHGDNLDGRGMIAFAFNPIPANFSDPGTIAQLQETFVFWRVAKGGMGLPREGFPWASA
                     MPPWEQHLTTDEIWKVVMFEYWHTNFFPRTWD"
     gene            2840..>3045
                     /locus_tag="VNV63_01070"
     CDS             2840..>3045
                     /locus_tag="VNV63_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61239.1"
                     /translation="MSGSKTKFLIAGLTVFLVAVPTVIITLAQTEKTAPQVNRPAKPS
                     EEDLKAGEQIYFRKCVWCHGEKGDG"
BASE COUNT          715 a          748 c          827 g          755 t
ORIGIN      
        1 aattggtggt tggccatcta tggatactat attcccgcaa atgtccgaat cgggctctcg
       61 gtgccgcagg ttttaggcac cctgacctgc ctttttgctg ggtgcggcat caatatcgcc
      121 atgatgaaag ggtcaaagcc caccggtccc gtggagtggg gcaagatctc agtaagatct
      181 cagtatgctc tgtttgcctt gggagtatcc tttacctggc tgatggcctt gatggggtat
      241 atccggtcct ccgtgcggtt gttctggcac gtgatggaag tcttccgaga cagctcccct
      301 tgggcgttta cccacacgat cgggtttgcg gccaatgtga tgtcttttaa tgtgttagtg
      361 ttttgggtgt tttgcctgtt tgtgttctgg ttgggcgagc ttacaacagc gcatgcggtc
      421 aaagaagcca agcggggaaa ggttattggc gagaccgtca tcgcaggcgg ttctgagtaa
      481 gcccatccgc taacgcaaac agtccatttg tgaatccgga ggtcagtctg caaaacgtgg
      541 cggctggcct cccttcctgc ctggaggagg ataagacgtg actcagacct tgtttgccac
      601 ttcaatatcg ctgttagcgc ttttcttttt gctccttggc tactttgtcc cacgagccaa
      661 ggtactcatg ttcagcatcg gtttctattt tgtcatgacc gcgctcttcg ccggttattc
      721 caactggctc ccacaagttc gcggcgaagt gccccaagag accaagattg atccaggcga
      781 tatattgaat atgccgaaag acaagctcgt cgacatggga gagacgatca tcttcggagg
      841 agtcggagga atggattcac ggaggactga tgggaaggga caatgtcctc tctgccatac
      901 ctttaagaag ggcgacattg gagaccgtgc tccgaatctc ttgggaatcg gtactcgagc
      961 tgctcagcgg atcaaggacc caagatatct gaaaccgaac acggttcaga ccgaggcttt
     1021 tcccggaagc ggacgggcca cgacggccga agagtatatt gccgagtcgc attcctgccc
     1081 ctcctgcttt gtagtggaag ggtttggtgt caaggggacg aatgatcgtg agagcccaat
     1141 gccgacaatc cataagccac cggtcagcct ttcgatcccg gagcttatcg cggtagacac
     1201 ttttctctgg gcacggctaa gccctgaaga agcaacgcct tctcctgccg aaataaaagc
     1261 ggcgtatgag aagttcattc caccggcaga gcgtcagcaa ggggccccaa ccgctgttac
     1321 gacaagcgcg ccggcaccgg ctggtcctcc aattgcattg ggaacggata ctccggaaca
     1381 gatcatcacg aagatgggct gttttgcctg tcatcagatt ccgaccattg cggcggctaa
     1441 gttcggacca attggaccgt tactggttga aaagacaaat gcgccaaaga gaattgcatc
     1501 acccgaatac caggcgatgt tgaaagccgg caaagcccat gccaggacac caaaggaata
     1561 tgttatggaa tctatcgtga ccccgaatgc ctttattgtc catgcttttg tgcagaaggc
     1621 caacccggat acatcggata tgatccagga tttttcgcag aagttcacgt ttggggcctt
     1681 ggaaaagctg gctgattttc ttctaacgct tgattgtgat gcggcgaaga aagatgggct
     1741 caagggtcct cctgcggaac cgattgacca ggtgtgcggt aagggaccgg agaagaccgc
     1801 ctccttggcc caataaaaat tttacagcga tctttggaac gatttaaaac ttttaggagg
     1861 taaaacagta atgaagatct ggattcctgg gtacatgccg atccaactgc tcttgatctt
     1921 cggtctcttt ctggttcttt ccctgggggt gggaacgctg tacaggcgaa ttactggaag
     1981 gccgcttcac atgctgatcc agggcaccgg agcttggttg atcgcctatt ttgtccttcg
     2041 atttgttttg tacccgccga tcccgctcag tgtcttttcg acctacatgg gactgatcac
     2101 gctcgtgatc ttcctttata tgtcttcgac cgaggccggc tggcaggaaa cccaaaaacc
     2161 gattctcgat acgctcaaag gagagacgca gatatatcgc ggtgtccgag cggtaacatt
     2221 tgtagccttg cctatactga ttggatatgg aacatttagt caagtggtgc ctaaagtgga
     2281 tgagccgatt gagttgcgga ccgtgcatcc ggcccctcct caaaacacta cggtccacgg
     2341 caacctgttt actctgcaaa cagcacagaa cccctatcgt gtggatgacg aaggcaagta
     2401 ccttccggta ggaaaagaag acaaatattt taataagaat gccttcgatc cgaatgcgcc
     2461 taaatttttg cagtatgtga aagagggcgg gcaggtcttt tttaacacag ccggttgctt
     2521 catgtgccac ggggataatc tcgatgggcg gggaatgatc gcttttgcct ttaaccccat
     2581 cccggcaaac ttctctgatc cgggcacgat cgcacagctt caagagacgt ttgtgttctg
     2641 gcgtgtggcc aaaggcggga tgggacttcc acgtgaagga tttccctggg cctccgcgat
     2701 gccgccttgg gaacaacact taaccacaga tgaaatatgg aaggtcgtga tgtttgagta
     2761 ctggcacacc aacttcttcc ctaggacctg ggattaagat tttataaaac gactgaactt
     2821 ttacggagga caaggaatga tgagcgggtc taaaactaaa tttctgatcg ctgggttaac
     2881 ggtcttcctt gtagccgttc ccaccgtgat catcacatta gcccaaactg aaaagaccgc
     2941 accgcaggtc aatcggccag ccaagccctc ggaggaggat ctcaaggcgg gtgagcagat
     3001 ctattttaga aagtgtgtct ggtgtcatgg tgagaaaggg gatgg
//
LOCUS       DATMKH010000090         1048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4216391, whole genome shotgun sequence.
ACCESSION   DATMKH010000090 DATMKH010000000
VERSION     DATMKH010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4216391"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..966)
                     /locus_tag="VNV63_01075"
     CDS             complement(<1..966)
                     /locus_tag="VNV63_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61240.1"
                     /translation="MLKQIATFPSPPIHLSGLAVLFFVFVPVICFAQRLTPLSDPPDW
                     SKLNRFQKTITHDEFVDLLNSVYAPNGAGAQWIKVEATEAVIQENASEHFVLQFASSA
                     EVSKPVPRYWTTAASLTPTPNRKLPLSGVKLAIDPGHLGGIWAKMEERWFQIGDSVPV
                     TEGDLTLRVAQILAPRLESLGAKVSLVRSAPGPVTTVRPEALKTQAEASLRERGVTNP
                     FPSYQGPDDPTREDSVPWEAERLFYRVDEIHARAEIVNERLEPDLVLCLHFNAEPWGS
                     AAQPVLVSANHLHLLINGAYSSIELGFDDQRFAMLFKLLNRTYPEE"
BASE COUNT          246 a          282 c          291 g          229 t
ORIGIN      
        1 ctcttcggga taggtccggt tcagaagctt gaacagcatc gcgaaccgct ggtcatcaaa
       61 accaagctcg atggagctgt acgcgccatt gatcaataag tggagatgat tcgccgacac
      121 aaggaccggt tgagcggcgg atccccaggg ttccgcgtta aagtgaaggc agagcaccag
      181 gtccggttcg agtctttcat tgacgatttc cgcgcgcgcg tggatctcgt cgacccgata
      241 aaataatctc tcggcttccc atggaaccga gtcttcgcga gtcgggtcgt ccggtccctg
      301 ataggacggg aaaggattcg taacccctct ctctcgcaat gaagcctcgg cctgggtttt
      361 cagcgcttcg gggcgaacag tggtgaccgg accgggagcc gatcggacca gggagacttt
      421 tgctcccagg ctctccaggc ggggagcgag aatctgggcg acacgcaacg tcaagtctcc
      481 ctccgttaca ggaacggagt ccccgatttg aaaccagcgt tcttccatct tggcccagat
      541 tccacccaga tgacccggat cgatggcaag tttcactcca ctaaggggca atttcctgtt
      601 cggagtcggc gtcaaactcg ccgccgtcgt ccaataccgc ggaaccggtt tgcttacctc
      661 agcgctcgag gcgaactgaa gaacgaaatg ctctgaagca ttttcctgga taaccgcctc
      721 agttgcttcg actttgatcc attgcgctcc cgctccgttc ggggcgtaca ccgaattgag
      781 cagatcgaca aactcgtcat gggtgatcgt tttttggaat ctattcagct tactccaatc
      841 cggaggatca gagagtgggg tcaaccgttg ggcgaaacaa ataaccggga cgaaaacgaa
      901 aaacaggaca gccaagccgg agagatgaat cgggggcgaa ggaaacgtag caatttgctt
      961 cagcatctac gagatcttat ataagacgta atcaaggtgt attcatcagg gtgggcgcaa
     1021 tgcttcgact tttatttctc ggcgacat
//
LOCUS       DATMKH010000091         1053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4529924, whole genome shotgun sequence.
ACCESSION   DATMKH010000091 DATMKH010000000
VERSION     DATMKH010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4529924"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="VNV63_01080"
     CDS             complement(<1..511)
                     /locus_tag="VNV63_01080"
                     /inference="COORDINATES: protein motif:HMM:NF014766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HXC61241.1"
                     /translation="MLHIELPLLSPFRTSLGVEENRSALILIYKSNGITAFSECATFE
                     NPYYTSEDNKTAIHVIQDHLAKGLPTEPSPGEFLERVALIKGHNMAKAAVEMLLWDYH
                     CKARKEPLARALGKSKGYAEIGISLGIDSVDRLLEKVTAARDRGYKRIKIKIKRGREY
                     ELVHAVRDSF"
     gene            complement(547..>1053)
                     /locus_tag="VNV63_01085"
     CDS             complement(547..>1053)
                     /locus_tag="VNV63_01085"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HXC61242.1"
                     /translation="AILGGIFYSEGPLPVSSTPFGELLVALLMGPIEIVSADLAASGR
                     VSSLAFVVSIPVGLMVAAILLTNNLRDIDKDRSRGRRTLAVLIGRRSGANLLLIMLVL
                     TFLWTIPAFALFAISASVFLIWLAFPIALQSYYRIIHGGWSRSVVLISRVHVLVGTLL
                     TISLLVHL"
BASE COUNT          276 a          275 c          270 g          232 t
ORIGIN      
        1 gaaaggaatc tcttactgca tgaaccagct cgtattctct tccccgtttg attttgatct
       61 taattctctt atagcccctg tcccgtgccg ctgtcacttt ctcgagcaac ctatcaactg
      121 agtctatacc aagggaaatc ccgatctccg cgtatccttt cgatttgccc agcgcccgag
      181 caaggggttc ctttcttgcc ttgcagtgat agtcccacag gagcatttcc accgcggctt
      241 tcgccatgtt gtgtcctttg atcagagcga cacgctccaa aaactctccc ggggacggtt
      301 ccgtcggcaa acctttagca agatgatcct ggattacgtg aattgcggtc ttgttatcct
      361 cggacgtata gtaaggattt tcaaaagtag cacattcaga aaaagccgtg ataccattgg
      421 atttatagat gagaattagg gcacttctat tttcctcaac tccgaggctt gtcctgaagg
      481 ggctcaacaa gggtaattcg atgtggagca cttctagagt tggagagcgc aagaagttct
      541 ttcccctcac aagtgcacaa gaagggaaat ggtgagcaaa gttcccacga ggacgtgaac
      601 cctcgaaatc agaacaaccg acctggacca acccccgtgg attatgcggt aataactctg
      661 taacgcgatc ggaaatgcga gccagatcag gaacacggaa gcggagatcg caaacaacgc
      721 gaaagctgga atggtccaga ggaaggtaag tactagcatg attaggagca agttcgcacc
      781 cgaacggcgg ccaatcaata ctgcgagcgt tcttcggccg cggctgcgat ccttgtcaat
      841 gtcgcggaga ttgttggtca agaggattgc cgccaccatc aggccgacag ggatcgagac
      901 gacaaaagca agactcgaca cccttccaga ggcggccaga tcggcggaaa ctatctcaat
      961 cggtcccata aggagagcaa cgaggagttc cccgaaaggg gtggaagaca ccggcagcgg
     1021 gccctcggag tagaaaatcc ctcccaggat tgc
//
LOCUS       DATMKH010000092         2801 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3895216, whole genome shotgun sequence.
ACCESSION   DATMKH010000092 DATMKH010000000
VERSION     DATMKH010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3895216"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..443
                     /gene="rpsH"
                     /locus_tag="VNV63_01090"
     CDS             48..443
                     /gene="rpsH"
                     /locus_tag="VNV63_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014465724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HXC61243.1"
                     /translation="MNNDPIADMLSRIRNAFLRRHDTVEMPASKMKIEIAKILKTEGF
                     IRDFLKESEGPQATLKLRLKYMDGHRCVIQGLERVSKSGRRVYVGKDEIPRVKGGVGI
                     AILTTPQGLMTDQEARQKKIGGEVICHVW"
     gene            461..997
                     /gene="rplF"
                     /locus_tag="VNV63_01095"
     CDS             461..997
                     /gene="rplF"
                     /locus_tag="VNV63_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011293054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HXC61244.1"
                     /translation="MSRVGKKAIAIPGNVKVKIEDREILVHGPKGELKRQIPPSIQAT
                     ITEGQLVFSRISEERHVRSLHGLTRSEVANMITGVTLGYSKVLEINGVGYRAQMKGRT
                     IMLSIGFSHPVNFELPNGIEASVEKQTILTIQGIDKYLVGQVSARLRSLRPPEPYKGK
                     GIKYQAEKIIRKEGKTGK"
     gene            1009..1371
                     /gene="rplR"
                     /locus_tag="VNV63_01100"
     CDS             1009..1371
                     /gene="rplR"
                     /locus_tag="VNV63_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020155528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HXC61245.1"
                     /translation="MSTDKKILARNRRHHRVRKKIVGTLERPRLSVFRSNSHLYAQVI
                     NDLVGKTIASASTSDKELGKKVKSTTIEAAKKVGELVAKRTLACGVKTVVFDRGGYLY
                     HGKVKSLAEAAREAGLIF"
     gene            1380..1880
                     /gene="rpsE"
                     /locus_tag="VNV63_01105"
     CDS             1380..1880
                     /gene="rpsE"
                     /locus_tag="VNV63_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013290166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HXC61246.1"
                     /translation="MADRQDLEEQGLKDKVIFINRVAKVVKGGKRFSFSALVVVGDKQ
                     GSIGVGHGKAAEVPEAIRKAVEKAKKQMIKIALKNTTILYTVMGHFGAEDVLLKPASE
                     GTGIIAGGPVRAVMDVLGVQNIISKSLGSGNPNNVVHATLHALKQLRDPEELKRSRQQ
                     DFQEAD"
     gene            1882..2073
                     /gene="rpmD"
                     /locus_tag="VNV63_01110"
     CDS             1882..2073
                     /gene="rpmD"
                     /locus_tag="VNV63_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HXC61247.1"
                     /translation="MASKKKLAITLVRSYIGYSYRQRLVVKGLGLRRLRETVIRENTP
                     SVQGMVRKISHLVKVEEVA"
     gene            2087..2545
                     /gene="rplO"
                     /locus_tag="VNV63_01115"
     CDS             2087..2545
                     /gene="rplO"
                     /locus_tag="VNV63_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HXC61248.1"
                     /translation="MKRLTLETLKHAPGSRQKPRRVGRGIGSGLGKTSGKGHKGQLAR
                     SGGGKRPGFEGGQMPLTRRVPKRGFKNPFRKEYAIVNLGNLERFDGKEPVTPDALKAV
                     GLIPGSAVAVKILGEGVIKKALTIQAHKFSKSAVQKIQAQGGKAEVLESV"
     gene            2538..>2801
                     /locus_tag="VNV63_01120"
     CDS             2538..>2801
                     /locus_tag="VNV63_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HXC61249.1"
                     /translation="MFERLITSIQNIFNIPELRTRVLFTGAMLAVYRVGVHIPTPGIN
                     SEALSQFLKAKGGALLGFLDIFSGGALFKLTIFALGIMPYISAS"
BASE COUNT          772 a          614 c          824 g          591 t
ORIGIN      
        1 aatcccggga gtgatcaagg cgagttggtg ataaaggagc tagagaagtg aacaacgacc
       61 cgattgctga tatgctgagt cgaattcgga atgccttctt aaggaggcat gatacggtag
      121 agatgccggc ctcaaagatg aagatcgaaa tcgcaaagat tctcaagacc gaaggtttta
      181 ttcgggactt tcttaaagag tccgaaggcc cgcaggcgac cctgaagctc aggttaaaat
      241 acatggatgg acatcgctgt gtcattcagg ggttggaacg tgtgagcaaa tccggaagac
      301 gggtttatgt gggcaaggat gaaataccac gggtcaaggg aggggtgggc atcgccattc
      361 tgaccacgcc tcaggggctg atgacggatc aggaggcccg ccaaaagaag attggcggtg
      421 aagttatctg tcatgtctgg taagatcgag cgagactgaa atgtccaggg taggtaaaaa
      481 agccatcgct ataccaggta atgtcaaggt caagattgaa gaccgtgaga ttttagtcca
      541 tgggccgaaa ggcgagctaa agaggcagat acctccatcg atccaggcga ccatcacgga
      601 gggacagctg gtatttagcc gtatctcgga ggaacgtcat gttcggtctc tgcatggcct
      661 cacccggagt gaagtagcca acatgatcac cggggtgaca ctgggctatt ccaaagttct
      721 ggagatcaac ggtgtgggtt atcgtgcgca gatgaaaggg cggacgataa tgttgagcat
      781 cggcttctca catccggtga attttgaatt gccaaacggg atcgaagcga gtgtcgagaa
      841 acagacgata ctgacgatac aagggattga taaatatctc gtgggacaag tcagtgcccg
      901 gctgcgttct ctccgtccgc cagaacctta caaaggaaaa gggatcaagt accaagctga
      961 aaagattatt cgcaaagagg gcaagaccgg gaaatagaga ttcaggatat gagcaccgat
     1021 aaaaaaatac ttgccagaaa tcgacgacat cacagggtcc ggaaaaagat tgtcggtact
     1081 ttggagcgtc cacggctcag cgtctttcgg agcaattctc atctctacgc ccaggtaatc
     1141 aatgatctgg tgggaaagac gattgcaagt gcctcgacct cggacaaaga actggggaaa
     1201 aaagttaaat ccacaacgat tgaggccgca aagaaggttg gagagctagt tgcgaaacgg
     1261 accctcgcat gcggcgtcaa gaccgtcgtg tttgatcgag gtggctatct ctatcatgga
     1321 aaagtcaaga gtcttgctga ggctgcccgc gaagcgggtc tgatctttta gaggtaaaaa
     1381 tggctgatcg tcaggatctg gaagaacagg gtttaaagga caaggtgatc tttataaacc
     1441 gggtcgctaa ggttgtcaaa ggcggcaagc ggtttagttt tagcgccttg gtcgtcgtgg
     1501 gagataagca aggctcgatc ggggttggtc acgggaaagc ggccgaggtt cccgaggcga
     1561 ttcgaaaagc cgtcgagaag gcaaagaagc agatgatcaa gattgcactt aaaaacacca
     1621 ctatcctgta tacggtgatg ggccatttcg gtgcagagga tgtcctgctc aagccggcat
     1681 cggaaggaac cggcatcatt gcgggagggc cggttcgggc ggtaatggat gttttaggtg
     1741 ttcaaaatat catcagcaaa tcgctcggaa gcggcaaccc gaacaatgtt gtacatgcca
     1801 ctctccatgc cttaaagcaa ttaagagatc ccgaagagtt gaagagatcg agacaacagg
     1861 attttcaaga agctgattga catggcatcg aaaaagaaac ttgcgattac cttggttaga
     1921 agttatatcg gctattccta tcggcaacga ttggttgtaa agggattggg acttcgcaga
     1981 cttcgagaga ccgtgatccg tgaaaacacc ccatcggttc aggggatggt caggaagatc
     2041 tctcacctcg tgaaggtgga agaggtggca tgaagagaat cgtaagatga agcgcctaac
     2101 actcgaaaca ctcaaacacg ctccagggtc gaggcaaaag ccgagacggg tcggcagagg
     2161 tatcggttct gggctcggca agacctccgg aaaaggtcac aagggtcagc ttgcaagatc
     2221 cggcggaggc aaacgccccg ggtttgaggg aggacagatg cctttgaccc gccgcgtccc
     2281 gaagcggggt ttcaaaaatc cgttccgaaa agagtacgcg atcgtcaatc ttgggaacct
     2341 tgagcgattt gatggaaagg aacccgtgac gccggatgcc ctcaaagcgg ttggattaat
     2401 acctggatct gccgttgcgg tcaagatctt gggtgagggg gtgatcaaaa aggcgctgac
     2461 gattcaggcc cacaagttta gcaagtcggc ggttcaaaag atccaggccc aaggcggaaa
     2521 ggccgaggtg ctggaaagtg tttgaacgac tcattaccag cattcagaac atttttaata
     2581 tcccagagtt gcggacccgc gtgctcttca cgggcgccat gctcgcagtc tatcgggtcg
     2641 gcgtgcatat ccccaccccg gggattaata gcgaggcctt gagtcaattc ttaaaggcta
     2701 aaggaggcgc ccttttagga tttctggaca tcttctccgg tggggccttg ttcaagctca
     2761 caatctttgc cctcgggatc atgccttaca tcagcgcctc g
//
LOCUS       DATMKH010000093         1446 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1969930, whole genome shotgun sequence.
ACCESSION   DATMKH010000093 DATMKH010000000
VERSION     DATMKH010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1446
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1969930"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            822..>1446
                     /locus_tag="VNV63_01125"
     CDS             822..>1446
                     /locus_tag="VNV63_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression protein HypE"
                     /protein_id="HXC61250.1"
                     /translation="MVEAGIRRGSQSSIPELDVLWITAGLGCDGDTIAMTAATQPSIE
                     DILLGQIPWTPKVTLHNPFLALANGYDFMKAFHQAAEGKMENFILVVEGSIPNEHNKG
                     EGYWASLGTDPKTGQPITTCEWIDRLAPQAWAVVAAGTCATYGGIHAMEGNPTGCMGL
                     PDYLGWKWKSRAEIPIVCVPGCPVQPDNFMETLLYLLYMASGRAPMIP"
BASE COUNT          344 a          391 c          399 g          312 t
ORIGIN      
        1 ggacagggga aggacgccga aggcgtacaa ggaacgcgaa atcccgattc ctccgaggct
       61 cgtgaagagc ctaaaggcat ggaaggcgaa agcgaataag agctgtaatc tggtattccc
      121 cacctcagga tgcaacccga agctcaactt actcaacaat ctgaaagccg tggccgaacg
      181 cgcgcagctt gatccagaga atttctggct acacaagttt cgagcgacct tcgcgacgcg
      241 gtgtctgtgg gcaggagtcg atctgcggac ggtgcagaca agtagacggg cgaggtgttt
      301 cttaacatga cttgtgtctt tcaagcctcc gatgctctcc gaggactcgt ggcgaccagt
      361 cggaggactc gactcctgtc tcacatcctg ctgatcctaa cgtagcggcc caggtctgga
      421 attgttttga gaaacacata tcccgcgaaa agcaacgttg ctatcccaat gccactcgtg
      481 attcttaaca atgttttcac aaggtctcct ggcaataatc ttacgcttgg atgtctttct
      541 cggagaaaga gcagcccaaa aaacaccata agccgaagaa aagcttgctc tgccttggtt
      601 ctggccgatc aaggatcagg tgttcttatc gcctccacgc tgtctgccga cttcggtaga
      661 gcgagaacga gagagaatcc gctaaaatat gccagattag caggcgactc taacgtgggg
      721 ttactctacc cgctcaactg tcggtccaga attcttccca tctcgatttg ggaaggaaga
      781 atcggaacgg agtctctggc gtgctccaag gagaaatcga aatggtggag gccgggattc
      841 gccggggaag tcagagttcc atccccgaac tcgatgttct ctggattact gccggccttg
      901 gatgtgacgg ggacaccatc gccatgacgg ccgccaccca gcccagtatt gaagatatcc
      961 ttctcgggca aatcccctgg actccaaaag tgaccctgca caatcccttc cttgccctgg
     1021 ccaacggcta cgacttcatg aaggcctttc atcaagcggc tgaagggaag atggaaaatt
     1081 tcattttggt ggtcgaaggg tccatcccga acgaacacaa taaaggagaa ggctattggg
     1141 cttccctggg caccgacccg aagactggtc agccgatcac cacgtgtgaa tggatcgatc
     1201 gtctcgctcc ccaagcgtgg gcggtagtgg cggcgggaac ctgcgctacc tatggcggta
     1261 tccacgccat ggaaggcaat cccacggggt gtatgggtct tcccgactac ttggggtgga
     1321 aatggaagtc gcgagcggag attcccattg tgtgcgtgcc cggatgcccg gtgcagcccg
     1381 ataatttcat ggaaacgctt ttgtatttgc tctacatggc atccggccgc gcgcccatga
     1441 ttccgc
//
LOCUS       DATMKH010000094         1885 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4216808, whole genome shotgun sequence.
ACCESSION   DATMKH010000094 DATMKH010000000
VERSION     DATMKH010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4216808"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..734)
                     /locus_tag="VNV63_01130"
     CDS             complement(<1..734)
                     /locus_tag="VNV63_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011217248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HXC61251.1"
                     /translation="MSTHPYQDSRLSIAQRVSDLLERMTLPEKIAQMSSHWLILSADG
                     NHRIRTDPFCQTVTTADIESMLRTGVGQITRPLGTHPVNPKDGVRALNALQKFLVEET
                     RLGIPAIAHEECLTGLMVKGATLFPSALNYGSTWNPDLIEAVGQEIRKEAKQVGCHQG
                     LAPVLDVSRDVRWGRTEETMGEDPYLVGVLATRYVRGLQGEKRELLATLKHYVAHSFS
                     EGARNHAPVHLGFKELNDIFMLPFEMA"
     gene            979..1455
                     /locus_tag="VNV63_01135"
     CDS             979..1455
                     /locus_tag="VNV63_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61252.1"
                     /translation="MSFAWLHQKISLRTSIVSICAAVVITLIYLQICLSLSVSENDGD
                     APEICRLYLEQNGFVHDHFGRLQEALFVKDKSVAIIQKPNPGTVGLYTFRVSGTKAKG
                     TLQMLWDREVGNGALTVNAINITEESPYSRPITGHGPKTAAPQISVLPEGPLAIVF"
     gene            complement(1648..>1885)
                     /locus_tag="VNV63_01140"
     CDS             complement(1648..>1885)
                     /locus_tag="VNV63_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012466295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HXC61253.1"
                     /translation="KSQGSEFDNVLLILPEKDSPLLTRELIYTGLTRARTRVEVWFQE
                     SVLRAAVARYIQRGSGLRDRLSKTRSWEDQYLNL"
BASE COUNT          418 a          497 c          512 g          458 t
ORIGIN      
        1 gccatttcaa agggcagcat gaaaatatcg ttcagctctt tgaagccaag atgcaccggc
       61 gcatgattgc gagccccttc gctgaaggag tgggccacat agtgcttcaa agtggccagc
      121 agttcgcgtt tttccccctg caaccctctg acataccggg tggccagaac cccgaccaga
      181 tagggatctt ctcccatggt ttcctcggtg cgtccccagc gaacatcgcg ggaaacgtcc
      241 aaaacggggg ccaatccctg atggcatccg acttgttttg cctcttttcg aatttcctgg
      301 ccgaccgcct caatcaggtc cgggttccac gtcgatccat agttcaacgc tgaagggaag
      361 agcgtagctc ctttgaccat cagacccgtt aaacattcct cgtgagcgat cgccggaatg
      421 ccgagccggg tctcttcgac gaggaacttt tgcagggcgt tcagggcgcg gactccatct
      481 ttcggattga ccggatgcgt gcccagcggc cgggtgatct gcccgactcc ggttctgagc
      541 attgattcta tgtccgccgt ggtcacagtt tggcagaagg gatcggtgcg gatccgatga
      601 ttgccatcgg ccgataaaat cagccaatgg gaagacattt gggcgatttt ctccggcaac
      661 gtcatccgct ccagcaaatc ggagacgcgc tgtgcgatcg atagccgaga atcttgatag
      721 ggatgggtgg acatgggttg ttgagggccc aataaatgac taacaatccg tcatggaggt
      781 tcgcaggaaa atctttggga ccttccgggc catcattgga taacctttct cgaggcgaag
      841 atatcacagt ttaaatttct gatccgggtc cctttgttat ggaaacgacc gttcgttaca
      901 gtggattgac aacttccgcc ggatccctcc ttctttcgcc tcgctatctc cgaaatcggt
      961 cgaaggttcg tggtattcat gtctttcgcg tggcttcatc aaaagatctc cctcaggacc
     1021 tcgatcgtct cgatctgtgc tgcggtcgtg atcacgttga tctacctcca gatctgtcta
     1081 tccctgagcg tgtccgaaaa cgatggggac gcgccggaaa tttgccggct ctatcttgag
     1141 cagaacggtt ttgtccatga ccacttcggg cggttgcaag aggctctatt tgtcaaagac
     1201 aagagcgtgg cgattatcca gaagccgaat ccgggcaccg taggtcttta tactttcagg
     1261 gtttcaggaa ccaaggcgaa agggacttta caaatgttat gggaccggga agtggggaat
     1321 ggagcattga cggtcaacgc gatcaatatc actgaggaga gcccttactc ccgaccgatc
     1381 accggtcacg gaccgaaaac ggcagcgcct cagatttcag ttcttccgga gggaccattg
     1441 gcgatagtgt tttgaagtgt cttttggcct gaggtagaaa acgaagaggt aaggcgggac
     1501 gctttacggt gtccgttccg aatgattacg tcttctggga tggattccat ccaaccgcca
     1561 atgcacataa gctcgccgct gagtttatct tcaaatctgt gttttcgaga cgccaccacg
     1621 cgttgttgtc aggccgtaag cccttaccta aagattcaaa tattgatcct cccaggatcg
     1681 cgttttagag agccggtcgc gcaggccgga tccgcgctga atatagcggg ccacagcagc
     1741 gcgcaaaacc gattcctgaa accatacttc gaccctagtt cgtgctcggg tcaggcccgt
     1801 gtaaatgagt tcgcgcgtga ggagaggact gtctttttcg ggcagaatga gcaagacatt
     1861 gtcaaattct gagccctggc tctta
//
LOCUS       DATMKH010000095         2517 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2935785, whole genome shotgun sequence.
ACCESSION   DATMKH010000095 DATMKH010000000
VERSION     DATMKH010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2935785"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="VNV63_01145"
     CDS             complement(<1..627)
                     /locus_tag="VNV63_01145"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HXC61254.1"
                     /translation="MKIYFRLLNYLKPYWMYLAAASLSAAAVSGLTATQAWLVKPVLD
                     GIFINKDEWLLKVLPFAIIGVSLFKGLFNYAQAYWMSYAGNRVIKNIRDGLYRHLVLM
                     PVGFYTRHSTGELISRVLNDVGVMQMAVSSVVKNIFQQTLTLIALTVVVFYQNWRMAI
                     IAVLVIPLAYYPIMRLGRRLRKISRSGQERIADLSSSLQETFSGIRAVK"
     gene            complement(635..1771)
                     /gene="lpxB"
                     /locus_tag="VNV63_01150"
     CDS             complement(635..1771)
                     /gene="lpxB"
                     /locus_tag="VNV63_01150"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HXC61255.1"
                     /translation="MKVKTVLIISGETSGDLHGGNLAFALRKMRPELRLLGVGGPRMS
                     AAGVELIEDNREMGVIGVWEVLSHLKAIRRAYRRVLQVLASGQVDLLVLIDYPEFNFR
                     VAHVAHRKKIPIVYYISPQIWAWRSGRVKTVKKLVQQMIVILPFEKELYQQSGVPCEF
                     VGHPLLDEVQTDLDKKEVLRSFRLDPGQPVIGLLPGSRSKEVKKILPLMLAAMTRIGQ
                     KVPGIQLLLALAPSIEKDEVLAITSRWPLDIRIIEGKTDHVIAASDLVLVASGTATLQ
                     AAILETPMVILYRVAWLTYLLGRILIKVDHIGLVNLVAGRRIVPELLQSEATPERIAE
                     EIQGMLMDLPRREAIKKELAQVRSRLGTPGASQRAAEIILRHIA"
     gene            complement(1768..>2517)
                     /locus_tag="VNV63_01155"
     CDS             complement(1768..>2517)
                     /locus_tag="VNV63_01155"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXC61256.1"
                     /translation="VHVMLEKPMTQTLSEADHLIELAEKRKIVLQIGHLERFNSAIRM
                     LAGVICEPRFVEVHRLGGFADRGTDVDVVLDLMIHDIDILLSLVPSDVSEIRAAGTPV
                     ISQSIDIANARIEFKNGCVANVTASRVSREPMRKIRIFQPDAYLSLDYKNQELVVAKR
                     VQNAKGISFFSSLWRSSRVPGMPGIVVEKIKVKKEEPLKAELESFIQAVRFGEKPIVS
                     GREGREALKVALQVMEQIRLHTRRAQEAGRS"
BASE COUNT          596 a          729 c          613 g          579 t
ORIGIN      
        1 cttgaccgcc cggataccgg agaaggtctc ctgaaggctc gaagagaggt ccgcaatcct
       61 ttcctggccg gaacggctga tctttcgaag acgccgtccc agccgcataa tcggataata
      121 ggccagaggg atgaccaaga ccgcgatgat cgccatcctc cagttctgat agaagaccac
      181 gaccgtcaac gcgatcagcg tgagagtctg ttgaaagata ttcttgacga cgctggagac
      241 cgccatctgc atgactccga catcgttcag gacgcgggag ataagctcgc cggtcgaatg
      301 ccgggtgtaa aatccgaccg gcatcaggac tagatggcga taaaggccgt cccggatatt
      361 cttgatcaca cggttcccgg catagctcat ccaataagcc tgggcgtagt tgaacagtcc
      421 tttgaaaagt gaaacgccta tgatcgcgaa tggcaaaact ttgagcagcc actcatcttt
      481 attgatgaaa atcccgtcca gtaccggctt caccaaccag gcctgtgtcg ctgttaatcc
      541 ggagacggct gcggcgctga ggctggccgc ggcgagatac atccagtaag gtttaagata
      601 atttaataat cgaaaataga ttttcatccg ttactcatgc gatgtgccgt aagataatct
      661 cggctgcgcg ttgagaggcg ccgggagttc cgagccggga cctcacctgg gccaactcct
      721 tttttatcgc ttcccgacgc ggaagatcca tgagcatacc ttgaatttct tctgcgatac
      781 gttccggcgt tgcctcactt tgcaaaagtt ccggtacaat ccgtcttccg gccaccagat
      841 tgacgagacc gatatgatcc accttaatca gtattcgacc gaggagatac gtgagccatg
      901 ccacgcggta gagaatcacc attggagttt ccagtatcgc tgcttgcaac gtagcggttc
      961 cggaagccac caggaccagg tccgaggcgg cgattacgtg atcggtcttt ccctcgatga
     1021 tcctgatatc aagcggccag cgcgaggtaa tcgctaaaac ctcgtccttc tcaatcgaag
     1081 gcgccaaagc caggagaagt tgaatgcccg gtactttttg accgatccgt gtcattgctg
     1141 ccagcattaa gggcaggatc ttcttgacct ctttagaccg gcttcccggc aacaagccga
     1201 tgacgggttg tcccggatcg aggcgaaagc ttcttaaaac ctctttttta tctagatcgg
     1261 tttgaacctc atccagcaga ggatgaccca caaattcaca cggaactccc gattgctgat
     1321 acagctcttt ttcaaacgga aggattacga tcatctgttg gaccaatttt ttaacggttt
     1381 tgacccgtcc agatcgccaa gcccagatct gcggactgat atagtaaaca atcggtattt
     1441 ttttccggtg cgccacgtgc gcgactctga aattaaattc cggatagtcg atcagaacca
     1501 acaagtccac ttgcccggag gccagcacct gcaacacccg ccggtaagcc cgccgaattg
     1561 ccttaagatg agacagtacc tcccacaccc cgataacgcc catctctcga ttgtcctcga
     1621 ttaactccac gccagccgcg gacatcctgg ggcctccgac gcccaaaaga cgaagttcgg
     1681 gccgcatctt acggagggcg aaggccagat tccctccatg aaggtctccg gatgtttccc
     1741 ctgatatgat caaaactgtt ttgaccttca tgatcgaccg gcctcctgag ctcgacgcgt
     1801 atgcagccgg atctgctcca tcacctgcaa ggcaaccttg agcgcctccc gcccctctcg
     1861 gccggagacg atcggttttt ctccgaaacg caccgcttgg ataaacgact ccaattccgc
     1921 cttcaacggc tcctcttttt tcactttgat cttttcaacg acgatcccgg gcatgcccgg
     1981 gacgcgcgac gagcgccata aggatgagaa gaaactgatg cccttcgcgt tctgaacccg
     2041 tttggccacc acaagctcct ggtttttata atccaacgaa aggtaggcgt ccggttgaaa
     2101 tattcgaatc tttcgcattg gttcacgcga taccctgctg gccgtgacat tggcaacgca
     2161 gccgttttta aattcgattc ttgcattcgc gatgtcgatg gactgcgaaa tcaccggtgt
     2221 ccccgcggcc cgaatctcgg acacatcaga cggcaccagg cttaacagaa tgtctatgtc
     2281 gtggatcatc agatccagaa ccacgtccac atccgttccg cgatcggcaa agcctcccag
     2341 ccgatgcacc tcgacgaatc tcggctcgca tataacaccg gcaagcatcc ggatcgccga
     2401 gttaaaacgc tcgagatgac ctatctgtag aacgatctta cgcttctcgg ctaactcgat
     2461 gagatggtcc gcttccgata gtgtttgcgt catcggtttc tctaacatca catggac
//
LOCUS       DATMKH010000096         1481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1972526, whole genome shotgun sequence.
ACCESSION   DATMKH010000096 DATMKH010000000
VERSION     DATMKH010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1481
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1972526"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..281
                     /locus_tag="VNV63_01160"
     CDS             48..281
                     /locus_tag="VNV63_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HXC61257.1"
                     /translation="MKADVTLDTLGLRCPMPVILTTKKIKELEPGQVLEVLSDDQAIK
                     KDMPAWCHSTGNECLEIHEENGSYRVFVKKRQA"
     gene            complement(294..>1481)
                     /locus_tag="VNV63_01165"
     CDS             complement(294..>1481)
                     /locus_tag="VNV63_01165"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HXC61258.1"
                     /translation="NGDTRLGGDDFDRAMTEIILHEMESRYGNGIRQDPKLIERVRIA
                     AEKAKIELSFQLQTELVIALEDGKKSYRRTLTRNEIEDLIRDHVSKTAEPCRQAMADA
                     KLTPDQIDEVLLVGGSTRMPLVQQTVKALFKKQPKCELNPDEVVALGAAVQAHILMGE
                     IRDMLLLDVTPLSLGIETFGGIMSKLIERNSKVPTSAKETFTTFVEGQTIVDIHVLQG
                     ERELVNDNQSLARFALKGIPPMPAGIPRIEVTFHLDANGILNVSAKELHTGQEQSIEV
                     KPTYGLSKTEVERMIKDSMVHARSDIEARQLIETRNEADAIIRATEKALRDYSTLIHE
                     KEQKKITTALERLTLLLKSEDRHQIRQAIQEVETLTRPFAEAIMQKTVQEDLKGKKLS
                     DVL"
BASE COUNT          315 a          387 c          374 g          405 t
ORIGIN      
        1 ttgacaatcc tgttgtagct ggagtacttt ttaactaaac tattatgatg aaagcggatg
       61 tgacactcga tacgctgggt ttacgttgcc ccatgccggt tatcctgacc acgaagaaga
      121 tcaaggagct tgagcctggg caggtcttgg aggtgctgtc ggacgatcaa gcgatcaaga
      181 aagacatgcc cgcctggtgt catagtacag gaaatgaatg cctggagatc catgaggaga
      241 acggcagcta tcgggttttt gtcaaaaagc gtcaagcata aacccgttcg gtcttataaa
      301 acgtcggata gtttcttacc cttgagatct tcttgaactg tcttctgcat aatggcctca
      361 gcaaaaggac gtgtcagagt ctccacttct tggatcgcct gacggatctg gtggcggtcc
      421 tcgcttttta agagcaacgt tagccgttca agagcggtcg tgatcttctt ttgttccttc
      481 tcgtgtatta aggtgctgta atccctgagt gctttttcgg tggcccgaat gattgcatcg
      541 gcctcgttgc gagtctcgat caattgccgg gcctcaatgt cgcttcgggc atgcaccatc
      601 gagtctttaa tcatcctttc cacctcggtt ttgctcaagc cataagtcgg tttgacttcg
      661 atggactgtt cctgtccggt gtgaagttcc ttggcgctga cgtttagtat cccgttcgca
      721 tccagatgaa aggtgacctc aatccgaggg atgccggccg gcatcggtgg aatccctttc
      781 aaggcaaaac gggccagact ctggttgtca ttaacgagct cccgttcgcc ctgaaggaca
      841 tggatatcta cgatggtttg accttccaca aaggtggtaa aggtctcttt tgcactggtc
      901 gggacctttg aatttcgttc aatcagcttg ctcatgatgc ccccgaaggt ctcgatgcct
      961 aacgacagag gggttacatc caggagaagc atgtcccgaa tctcgcccat gagaatatgg
     1021 gcctgaaccg ctgcgcccag agcgaccacc tcgtccgggt tcaactcgca tttgggctgt
     1081 ttcttaaata aggccttcac ggtttgttgt acgagcggca tacgtgtcga gccgcctacc
     1141 agaagcacct catcaatttg atccggcgtc agcttggcgt ccgccatcgc ctgccggcaa
     1201 ggttctgcgg tcttgctgac gtgatcccga atcaggtctt ctatctcatt ccgagtgagg
     1261 gtccgacggt agcttttttt gccgtcttcc aaggcgatca caagttcggt ctgtaattga
     1321 aaggacaact ctatcttggc cttctcagcc gcaatccgaa cccgttcgat cagttttggg
     1381 tcctgccgaa taccgttccc ataccgactt tccatttcat gaagaatgat ctctgtcatc
     1441 gcacgatcga aatcgtcgcc gcccaagcgt gtatccccat t
//
LOCUS       DATMKH010000097         4986 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3257332, whole genome shotgun sequence.
ACCESSION   DATMKH010000097 DATMKH010000000
VERSION     DATMKH010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4986
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3257332"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VNV63_01170"
     CDS             complement(<1..294)
                     /locus_tag="VNV63_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXC61259.1"
                     /translation="MADIVVEVKELTKRYDKLIAVDRVSFEIYQGEILGLLGPNGAGK
                     TTMIQMLLGLLIPTTGEIKILGMDLARHREAILSQVNFSSAYVAMPYSLTVIEN"
     gene            415..831
                     /locus_tag="VNV63_01175"
     CDS             415..831
                     /locus_tag="VNV63_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61260.1"
                     /translation="MTINNKPPGIVSWAIIVSAASLLVTLPHAFEDFVYGVPQQFGLS
                     VMAAGFLVAIGYLLQLIGMLLALHGRQTGLVLLLLIGLGWTVGAMMDHLPEILQAGPY
                     RQGTISKVMELLIIQIGLVLVVLSAAAIRQLRRRNL"
     gene            complement(948..1328)
                     /locus_tag="VNV63_01180"
     CDS             complement(948..1328)
                     /locus_tag="VNV63_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HXC61261.1"
                     /translation="MSLLITAECINCGACLPECPNEAIFENRTAAEATGIRVTDGIGM
                     GDAIYVISYDRCTECVGHFDEPQCSAVCPVADCCIADPAWPESKEVLLEKAHRLNPDK
                     EIDPAKAWNSIRPGPLAGTTSPAA"
     gene            complement(1449..1970)
                     /locus_tag="VNV63_01185"
     CDS             complement(1449..1970)
                     /locus_tag="VNV63_01185"
                     /inference="COORDINATES: protein motif:HMM:NF017945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HXC61262.1"
                     /translation="MEMIYDHSFYLVLVRWVHFLAGVTWIGLLYYFNIVQVPFMKETD
                     PATKSAVVQKLVPRALWWFRYGALVTVLAGILLLQGHWQGFGNLFSSSWGIFISIGGL
                     LGLIMFFNVWVFIWPNQKQIIRMTTEAAANKTAPPPEMAKLARIAYLASRTNFVLSFP
                     MLFCMGAASHWSF"
     gene            complement(2063..3520)
                     /locus_tag="VNV63_01190"
     CDS             complement(2063..3520)
                     /locus_tag="VNV63_01190"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016872811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HXC61263.1"
                     /translation="MSIKMPVTKISENLWEVPRTAKKGMRVPARIYATEKLFEEIDDG
                     VFEQITNVACLPGIQKYAICMPDGHWGYGFPIGGVAAFSLDHGVISPGGIGFDINCGM
                     RLIRTNLTLPEVKPHLERLLEELFRTVPAGVGCQGTIKLSRSEFEEVMTHGVDWCIKK
                     GLGWPQDRERIEESGCIAGADPSKVSSQALNRGANQLGTLGSGNHYLEVQVASNDRIF
                     DRATAQALGIFGNDQVVIMVHCGSRGFGHQIGTDYLRVFDTAMQKYGITVPDRQLACA
                     PFRSPEGQDYYTAMRCAANTAFANRQVITHRIREVFEKLLGRSAEALGLEIIYDVAHN
                     VAKVERYKIDGKELNLLVHRKGSTRAFGPGRPEIPEIYRNVGQPVICGGSMETGSYLL
                     VGTEKAISETFGSTMHGSGRTMSRNAAKRSVSGKDLQRKMAQKGIIVKAASMSGLAEE
                     AGSAYKDISEVVKTVDSIGISKKVVELKPIGNIKG"
     gene            complement(3546..3974)
                     /locus_tag="VNV63_01195"
     CDS             complement(3546..3974)
                     /locus_tag="VNV63_01195"
                     /inference="COORDINATES: protein motif:HMM:NF001617.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HXC61264.1"
                     /translation="MGEFNFLEGIVTADVAVAVRGKTIEDLFATAALALFEVMVGTET
                     VQPQREKKLRLEGPIVDRLLYDWLSELVCLKDTDSMLFCRFDLVIKQENGYQLYATIA
                     GEEIDPKRHELRADVKAVTFHRFHVEERQGLWNAEIVFDI"
     gene            complement(4104..4757)
                     /locus_tag="VNV63_01200"
     CDS             complement(4104..4757)
                     /locus_tag="VNV63_01200"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HXC61265.1"
                     /translation="MYIPKIIPVFPLPDLVFFPKTYLPLHIFEPRYHKMVVDAKSGDQ
                     MIGMALLKEGWEEDYNQNPAIFPVGCVGRMIKVEPLEDRRFNIVLHGLKKFTIKDEFF
                     DRSYRQAAIELLGPRTETPVSIDTKLKDCLIQLLKEYGLLVKIEQQVDVLVKSNLDDE
                     ALVHTLSFVLNFTPLEKQFLLEAEDLDQQGHRLVDLIQFKIYEVRSGRRNPLPKKDS"
     gene            complement(4758..>4986)
                     /locus_tag="VNV63_01205"
     CDS             complement(4758..>4986)
                     /locus_tag="VNV63_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HXC61266.1"
                     /translation="YSFGCTLYRMVAERPPFTQGDVYQQHLSSLPVPPSAINADVPKA
                     LDLIILKCLEKDPQRRYSDVASLLRELEKIV"
BASE COUNT         1180 a         1330 c         1270 g         1206 t
ORIGIN      
        1 gttctcgatc acggtgagcg aataaggcat ggctacatag gccgaggaaa agttcacctg
       61 gctgaggatt gcttcccgat gccgggcgag atccatgccg aggattttaa tctcaccggt
      121 tgtagggatg agcaggccca gaagcatctg gatcattgtg gtcttgcctg cgccgtttgg
      181 accaagcagt ccgaggatct cgccctgata gatctcgaac gaaacccgat ccacggcgat
      241 taacttatcg taccgcttcg taagctcttt tacctcgacg acgatatccg ccattgttac
      301 ccctttagat ctcatgatac cccatcatgt ttcaggatgg caagaatcta tggtcttgag
      361 cagcttgatt ttccctgttg cctctgataa ggttccatcc taggggaaat agcgatgacg
      421 atcaacaaca agccgccggg tatcgtctca tgggccatca tcgtttcggc tgccagcctt
      481 ctggtgacgc tgcctcacgc ctttgaggat tttgtgtacg gcgtaccgca gcagtttggt
      541 ttgagtgtca tggcggcagg gtttctggtg gcaatcggat atcttctcca gttgatcgga
      601 atgttgctgg cattacatgg caggcagacg gggctggttc ttcttctctt aatcggtctt
      661 ggctggactg tgggtgcgat gatggatcac ctgccggaaa ttctccaggc cgggccttat
      721 cgtcaaggga cgatctccaa agtaatggag ttgctgatta tccagatcgg cctggtcctc
      781 gtagttctct ccgccgcagc gatcaggcaa ctccgaagga ggaatcttta agtaaggaaa
      841 caaaaaagcg agatatcaat ctcgcctttt ggggataaac aagtttttag ccgctattgg
      901 gtaaaacgat taatgcgaat gctacgttag ggttttgata agatgcgtca agcagcggga
      961 ctcgtcgttc ctgccaacgg ccctggccgt atactattcc aagccttggc aggatcaatt
     1021 tccttatcgg ggttcagccg atgggccttc tccagcagga cctccttgga ctctggccaa
     1081 gccggatcag caatgcagca gtcggcaacg ggacaaaccg ccgagcactg aggctcatca
     1141 aaatggccta cacactcggt acaccgatca tacgaaatca catagatggc gtcacccatt
     1201 ccaattccat ctgttacccg aatgccagtc gcctctgcgg ctgtccggtt ctcgaaaatg
     1261 gcttcgttgg ggcattcagg aagacaggca ccgcagttga tgcactccgc tgtgatcaac
     1321 agtgacatag gatcaactcc tcctcaatta agaactaggg agtgaaaccc atagttctta
     1381 ttacgtaaga tacattgtaa ccgatatgtg ttaccatcgt caagcgtttt tatgaacact
     1441 tccaggcctt agaaactcca gtggctagcg gcacccatgc aaaagagcat cgggaatgaa
     1501 agaacgaagt tcgtcctgga tgcgagataa gcgattctgg caagctttgc catctcgggt
     1561 gggggtgctg tcttatttgc agccgcctcg gtggtcattc gaataatctg tttctgattc
     1621 ggccaaataa agacccagac attgaagaac atgataagcc ccagaagacc cccgatcgat
     1681 atgaatattc cccaggaact gctgaagagg ttcccaaatc cctgccagtg tccctgcaat
     1741 aagagaatgc ctgccaggac cgtgaccaac gccccgtacc tgaaccacca caacgcccgc
     1801 ggaactaatt tctgaacgac ggcgctcttc gtggcgggat cggtttcttt cataaagggg
     1861 acctggacga tgttgaagta ataaaggagg ccaatccagg tcacgcctgc caggaaatga
     1921 acccagcgca caagtaccaa ataaaacgaa tggtcataaa tcatctccat tattttttcc
     1981 ctccctaatg atcatcgtac aaaagtttga tcaggatagc ctatctttta gtgcattgtc
     2041 aacctgcacg agcaatttct cttcaacctt taatgttccc gatgggtttg agctccacca
     2101 cttttttgga gattcctata ctgtccacgg tcttgaccac ctctgagata tctttgtaag
     2161 cggatccagc ctcttccgcc aggccggaca tcgaggctgc cttgacgatg atacctttct
     2221 gagccatctt tctctgaaga tctttgccac tgaccgaccg cttggccgcg ttgcgcgaca
     2281 ttgtccgacc ggagccatgc attgtagagc cgaaggtttc cgatatggct ttttcggtgc
     2341 cgaccaaaag gtacgatccg gtctccatcg agcccccaca gattacgggt tgtccaacgt
     2401 tccgatagat ctccgggatc tccggccggc ccggtccgaa ggcccgtgtg gagcctttcc
     2461 gatgcacaag caaattcagc tctttaccat caattttata ccgttccacc ttcgcgacgt
     2521 tgtgggccac atcatagata atctcaaggc cgagtgcttc cgctgaacgg cccaaaagct
     2581 tttcgaatac ctcccgaatc cggtgtgtga tcacctgacg attagcaaag gcggtgttgg
     2641 cggcgcatcg catcgcggtg taatagtcct ggccctcggg tgaccgaaac ggtgcacagg
     2701 cgagctggcg atccggcacg gtaatcccgt acttttgcat ggcggtatca aaaacccgga
     2761 gatagtctgt gccgatctga tggccgaatc cgcgggaacc gcaatggacc atgatgacaa
     2821 cctgatcatt accgaagatt cctaatgcct gagcggtggc tcgatcgaaa atccggtcat
     2881 tggatgcgac ctggacttcg aggtaatgat ttcccgaacc taatgtcccg agctggttgg
     2941 ccccccggtt caatgcctga gagctgacct tcgaaggatc ggccccggcg atacaacctg
     3001 attcttcgat tcgctcccga tcttgcggcc agcccagtcc cttcttgata caccagtcca
     3061 ccccatgggt catcacctct tcaaactctg aacgggagag tttgatcgtg ccctgacatc
     3121 ccacaccggc cggcacggtc cggaagagct cctccaacaa gcgttccagg tgtggtttga
     3181 cttccggaag cgtgaggtta gttcggatga gacgcatccc gcagttgatg tcaaatccga
     3241 tccctcccgg agagatcacg ccgtgatcga gcgaaaaggc ggcgacgcct ccgatgggga
     3301 agccgtagcc ccagtgacca tcgggcatgc agatcgcata tttctgaata ccgggcaggc
     3361 aggccacatt ggtgatctgt tcaaataccc cgtcatctat ttcttcaaaa agcttctcgg
     3421 ttgcatagat ccgggccggt acacgcatcc ccttctttgc agtccgcggc acctcccaga
     3481 gattttcaga aatcttcgtc accggcatct tgattgacat gaattgcact cccctgctga
     3541 attgtctaaa tatcgaagac gatctcggcg ttccaaagtc cttgtcgttc ttctacgtgg
     3601 aatcggtgaa aggtcacggc cttgacatcg gctcttaatt cgtgacgttt aggatcaatc
     3661 tcttcgcctg caatggttgc atacagttga tagccgttct cctgtttgat gacaaggtca
     3721 aaacggcaga aaagcataga gtcggtgtct ttcaggcaga ccagttccga cagccagtcg
     3781 tacagaagcc gatccacgat cgggccttcg aggcgaagct tcttctctcg ttgaggttga
     3841 acagtctctg tgccgaccat cacctcgaag agggccaaag ccgccgtggc aaagaggtct
     3901 tcgatcgttt tcccgcgaac cgccactgcc acatccgccg tgacgatccc ttcgagaaaa
     3961 ttaaactctc ccatgcacca ttatatgtac tcaaagagcc ggttttcaag gcatctcttt
     4021 acagcgaaca aagcgagcga gagggggagg ctccatcggg ctttgctgga ggagggggcg
     4081 acgtgagccc ctataataca aattcaactg tctttcttgg ggagaggatt gcggcggccg
     4141 gatcgtacct catagatctt gaactggatc agatccacca aacggtgtcc ttgctgatcc
     4201 aggtcttcgg cctcgagaag aaactgtttt tcaagggggg taaagtttaa aacaaaggaa
     4261 agtgtgtgga ccagagcctc atcgtccaga ttagacttaa caagcacatc gacctgttgc
     4321 tcgatcttca ccagaagccc gtactctttt aacagctgga taaggcagtc tttaagcttg
     4381 gtgtcgattg aaaccggagt ttctgtgcgg gggccgagca actcgattgc ggcctggcgg
     4441 taactgcgat caaaaaactc gtccttgata gtaaacttct tgagaccgtg caggacgata
     4501 ttaaaccggc gatcttctaa aggttccacc ttgatcatcc gaccgacaca acccaccggg
     4561 aagatcgctg gattttggtt atagtcctct tcccatccct ctttaaggag ggccatgccg
     4621 atcatctgat ctccggattt ggcatccacg accatcttat ggtaccgcgg ctcaaagata
     4681 tggagcggaa gataggtctt ggggaagaag accagatcgg gtaacggaaa gacaggtatg
     4741 atcttgggga tatacatcta cacaatcttc tccagctctc ggaggaggga tgcaacgtca
     4801 gaataccgcc gttgcgggtc tttttcaagg catttgagta tgatcaggtc caaggccttc
     4861 gggacatcag cgttgatggc ggagggtgga acaggcaggc tgctcaggtg ctgctgatag
     4921 acgtcccctt gtgtaaacgg cggccgttcg gcgaccatac gatacaacgt acagccgaat
     4981 gaataa
//
LOCUS       DATMKH010000098         2641 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3898559, whole genome shotgun sequence.
ACCESSION   DATMKH010000098 DATMKH010000000
VERSION     DATMKH010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3898559"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1519
                     /locus_tag="VNV63_01210"
     CDS             <1..1519
                     /locus_tag="VNV63_01210"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HXC61267.1"
                     /translation="IPDMTYLKMNRDKIRGVVLTHGHEDHIGALPYLLREIQVPVYGT
                     PLTLGLVAEKLKEYHLLSEAVLITVKPREIVKLGVFEVEFIQVTHSIVDGVGLGITTP
                     VGRVIHTGDFKVDQTPVDGKLMDLQKFGEYGDRGTLVLMSDSTNSEREGYTLSEREVG
                     KAFEEIFSQAPGRIIIATFASHIHRIQQAVDAAVKNGKRVIINGKSMLDNTRMAIELG
                     YLSLPAQTVAPIEDLSHLSPDQVVIITTGSQGEPRSSLSRMATNQHKQIQVQPGDTVI
                     FSSRVIPGNEQNIGRVIDQLFRLGAQVIYERVSEVHVSGHASQEEQKMMLNLVRPTYF
                     IPVHGEYRHLVHHTRLAERLHIPKENIFILDDGDVVEFHPDRAAKTGRVPVGRIFIDG
                     NRMGVVDEETLQERRRLGTHGLILVVLKVEKATGRLVQPPDLITRGFGALVILEEVAA
                     KVHEILEEQSKSNIDAAPIQTDWAAVQDQIKKRLRRFLLKKIERNPVILPIIIEV"
     gene            1521..>2641
                     /locus_tag="VNV63_01215"
     CDS             1521..>2641
                     /locus_tag="VNV63_01215"
                     /inference="COORDINATES: protein motif:HMM:NF024882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HXC61268.1"
                     /translation="MPANLTKENRRQEVIGVLLLALGLLLLISQLSYDPMDPSFTTLG
                     SERTVHNLAGRAGAYLTDALFQLLGASAYLITFFFLLTGWKLLFRRSDPIYRGLWRVG
                     GLFMLLFSFSAFSSLWLSALPTIRAGPTLIGKAGGLAGYLTTTLMLNYFALWGTHILV
                     TACLIASVMLTTSLSPIVVWDKAKDLLLLLKERVFTAVIIYREKSRRWKHLPTIASKL
                     SRPRPKISEAPPLVQAPPKQEKFEFVHPAGTSASGGSSEGSGGKGRYQLPPLSLLNDP
                     SVSTKKASKDDLLANSQLLERKLLDYGIEGKVTQVHPGPVVTLYEFEPAAGIKVNRIV
                     NLSDDLALGMRAMSVRIVAPIPGKSAVGIEIPNHVREDVS"
BASE COUNT          609 a          724 c          716 g          592 t
ORIGIN      
        1 catcccggat atgacctatc tgaaaatgaa ccgggacaag atccggggag tagtcctgac
       61 ccatggacat gaagaccaca tcggcgcgct tccctatctc cttcgagaaa tccaggtacc
      121 ggtctacggt actcctctca cgctcggctt ggttgccgag aagctgaaag agtatcatct
      181 tttatccgaa gcggttctga ttaccgtgaa accccgggag atcgtgaaac tcggagtgtt
      241 cgaggttgaa ttcatccagg tcacgcacag catcgtggac ggagtgggcc tcggcatcac
      301 gacgcctgtc ggtcgagtca tacacaccgg cgacttcaag gtggatcaga cgccggtgga
      361 tggaaagctc atggaccttc agaagttcgg ggagtacggc gatcgcggaa ccctggtttt
      421 aatgtcggac agtaccaact cggaacggga gggttacacc ctctcggaac gggaagtcgg
      481 caaggcattt gaagaaatct tctcgcaggc gccgggccgg atcatcatcg cgaccttcgc
      541 ctcccacatc caccggattc agcaggcggt ggatgcggcc gtaaaaaacg ggaaacgggt
      601 gatcatcaac ggtaaaagca tgctggacaa tacacgcatg gcgatcgagc tgggctatct
      661 ttcgctccct gctcagaccg ttgctcccat tgaggatctc tctcatctct cacccgatca
      721 ggtagtgatc atcaccaccg gaagtcaagg ggagccgagg tcttcgctct cccgtatggc
      781 cacgaaccag cacaaacaga ttcaggtcca accgggcgat accgtgattt tttcctcccg
      841 ggtcatcccg ggcaatgagc aaaacatcgg ccgggtcatc gatcaactgt tccgcctcgg
      901 cgcccaggtt atctatgagc gggtatcgga agtgcatgtt tcaggccacg caagtcaaga
      961 agagcagaaa atgatgctca acctggtaag gccgacttat tttatcccgg tccacggcga
     1021 gtatcgtcac ctggtgcatc atacccggct cgccgagcgt cttcatatcc ccaaagagaa
     1081 tatctttatt ttagacgatg gtgacgtggt cgagtttcat ccggatcgag cggcgaagac
     1141 cggacgggtc ccggtcggga ggatttttat cgatggaaat cgcatgggtg ttgtggacga
     1201 agaaaccctt caggaacggc gccggctggg aacccatgga ttgattctgg tcgtcctgaa
     1261 ggttgagaag gcaaccggcc ggctggtcca gcctcccgat ctcatcaccc gaggctttgg
     1321 agcccttgtg atattagaag aagttgcggc caaggtgcat gaaattctcg aggaacaatc
     1381 taagagcaac atagatgctg cccctataca aacggattgg gctgcggtcc aggaccagat
     1441 caagaaacgg cttcggagat ttttattaaa aaagatcgag cggaacccgg tcattctccc
     1501 gatcattatc gaagtttaag atgcccgcga acctcacaaa agaaaaccgt cgtcaagaag
     1561 tgatcggggt cctattgctc gctctgggac ttctattact catcagtcag ctttcgtatg
     1621 atccgatgga cccatccttt accaccttgg gttcggaaag gacggttcat aatctggccg
     1681 gccgggccgg cgcttacttg accgatgcgt tgttccagct cttgggagca agcgcctatc
     1741 tgatcacgtt ctttttcctt ctgactggtt ggaaactact ctttagacgt tccgacccga
     1801 tataccgggg cctctggagg gtcggtggtc ttttcatgtt gctattctct ttctcggctt
     1861 tttcctccct ctggttgagt gcccttccca cgattcgtgc tggaccgacc ctcattggaa
     1921 aggcgggcgg cttggcaggt tatctcacca cgaccctgat gctcaactac ttcgctttgt
     1981 ggggaacaca tatactggtc acggcctgtc ttatcgcttc tgtaatgctg accacatcgc
     2041 tctcaccaat cgttgtgtgg gacaaggcaa aagatctcct tcttttactg aaagagaggg
     2101 tcttcaccgc ggtgatcatt tatcgcgaaa agagccgccg atggaaacat ctgccgacga
     2161 tcgcgtcaaa attgagccgg ccgcgaccca agatcagcga ggcgccgccg cttgtccagg
     2221 ccccgcccaa gcaagaaaaa tttgagtttg tccatcccgc aggaacctcc gcctccggcg
     2281 gatcaagtga aggatcggga gggaagggca gataccaact cccgccctta agcctgctca
     2341 acgatccatc ggtctcgacc aagaaggctt cgaaggatga cctcctggca aattcccaac
     2401 tgctcgagag aaaactttta gattacggga tcgagggaaa ggtcacccaa gtccatcccg
     2461 gtccggtggt aacgctctac gagtttgaac ccgccgcggg catcaaggtt aatcggatcg
     2521 tcaatctatc ggatgatctc gccctgggca tgcgggcgat gagcgttcgg atcgtggcgc
     2581 cgattcctgg aaagtcggcg gtaggaatcg aaatcccgaa ccatgtccgc gaggacgtat
     2641 c
//
LOCUS       DATMKH010000099         1494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_371906,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000099 DATMKH010000000
VERSION     DATMKH010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1494
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371906"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            302..1494
                     /locus_tag="VNV63_01220"
                     /pseudo
     CDS             302..1494
                     /locus_tag="VNV63_01220"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
BASE COUNT          375 a          415 c          376 g          328 t
ORIGIN      
        1 gaattagaag cgaagactag gcccggttac caaaacgaga aaaccgattc gatcaaggag
       61 aacaacgcaa cgtcaactgg aagtagctta aaacccacaa cacgagatct cagaccgact
      121 tcatttgaag tttgtcattg atggcgagtg gaaggcgtcg tcaggcagtg ccaagctcga
      181 cctgacctct cctaactccg aggaactctt tctacgcgta cctgaagctt atccagagcc
      241 tcagggtcag ccacgcaaaa agaggctctc tcatgacggt ttgacgaaag gacctttttg
      301 tatgaaatca aatggagctc tcccgtctat acctcatccc aatcgcgagc aagcgctaaa
      361 aagggctcgt ctgaacgcgg ggtttccgaa tatagtgaac ggccaaagaa tcgactcttt
      421 gaagtcgata aagatctccg atcctgtaac tggagaagaa cttgcaagcg tgtccgacac
      481 ccagaaagat ggtctcgatc aggcggtcca ggcggcaaag gcagcgtttc ccctttggag
      541 cagcaagacc tggaacgaac gaagggatct gcttcgaaat gccatggaag agctcaaagc
      601 tcataccgat gagctttgca cgatcctgac agcggaaaac ggccggcctt atagcatggc
      661 gcaattcgag atcacatgga ttctcgagac ctatgtcccg gcgttacttc agatggaact
      721 gccagattcg acatggaatg aaaccggtgt cggacagatc acgaaacgtt atgttccact
      781 gggtgttgtc gccctgatca gtccgtggaa tcttctgttc ttgctgtcat tcacaaaagt
      841 gctcccatct ctgctgaccg gcaacaccgt tgtcctgaag cctgcggtct ttacccctct
      901 tacggtactt cgagccgccg actacattcg tgagatgctt ccgcccggcg ttttgaatct
      961 gatcagcgga ggggatgatc ttggcccctg aatgacgtcc cacccggagt tccagaagct
     1021 ctccttcaca ggatcttcgg aaaccggatg caaggtcttt gcatccgccg ctccaaccct
     1081 gaaacacctg acccttgagc taggcgggaa cgatcccggt attgtccttc cggatgccga
     1141 tcctcaggcg atcgccgagc acctgtttcg aagcatgttc ctactcagtg gcgaggggtg
     1201 tgtcaccctc aaacgccttt tcgtgcacga gagcatctat caagctctta cggaagcact
     1261 catatcctgt gctcaaaatc agaaactggg tgatgggttt gatcccgaga ccaccctggg
     1321 ccctatacag aaccgccccc agttcgctcg catgcaaacg acatgggaag agattcaaaa
     1381 ggcgaaggct cccatcctct atcaagggga agttccatcg gagggtgcag gcatgttttt
     1441 tcccgtcacc ctcctggaca atccgcctgc ggaagcggct tacgtaatcc gaga
//
LOCUS       DATMKH010000100         1260 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_691210,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000100 DATMKH010000000
VERSION     DATMKH010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_691210"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="VNV63_01225"
     CDS             <1..191
                     /locus_tag="VNV63_01225"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HXC61269.1"
                     /translation="FVLPDNKTKIQCTGEVVWKTRQDSSDDLAEGVGLRFADLNPKSQ
                     DVLDRWIEQRRGKTNKRR"
     gene            203..763
                     /locus_tag="VNV63_01230"
     CDS             203..763
                     /locus_tag="VNV63_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HXC61270.1"
                     /translation="MSQIRRIALLLISLSVLLPSFYYFKNYSAFHHPSTTESIPLSEQ
                     ILRVVDGDTIQLSNGEWVRYIGMDAPELRRKVGTRWVEDPQPGAVEAYELNRRLVEGK
                     RVRLELDVEQRDRFHRLLAYVFVGDLFVNAELVKAGYATVFTYPPNVRYHQLFLDLQK
                     KARVEHRGLWAKRMKQAAQPIGLLVG"
     gene            784..996
                     /locus_tag="VNV63_01235"
     CDS             784..996
                     /locus_tag="VNV63_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61271.1"
                     /translation="MKREQRKKKDRRDESEDRRAKERRKAERRSHDRREGNRRLEICP
                     TCGRVLTPSGYCSNCKVRVIKIRNSA"
     gene            1014..>1260
                     /locus_tag="VNV63_01240"
     CDS             1014..>1260
                     /locus_tag="VNV63_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020404433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlpA-like double-psi beta-barrel
                     domain-containing protein"
                     /protein_id="HXC61272.1"
                     /translation="MSERKGVFQCPLILPLLFLVSACATVQRPAEPEIGYREVGIASW
                     YGKEFHGRRTANGEIYDMNGLSAAHRTLPLGTIVNVTN"
BASE COUNT          313 a          294 c          369 g          284 t
ORIGIN      
        1 agtttgtttt acctgataac aagaccaaga tccagtgcac gggcgaggtc gtttggaaga
       61 cacgccagga ctcgtcggat gatttagcgg aaggtgtcgg actgcgattt gcagatctca
      121 atccgaagtc acaagacgtg ctggatcgtt ggatcgagca gaggagaggc aagacgaaca
      181 aaagacgatg aaaccgcacc gcatgagtca gatcaggcgg attgccctct tactcatcag
      241 tttgtccgta ctgctcccgt cattctatta cttcaaaaat tactctgcgt ttcatcatcc
      301 atccacgact gaatccatcc cgctcagcga acaaatcttg cgggtcgtgg atggggacac
      361 gattcagttg tctaacggcg aatgggtccg atatatcggg atggatgcac cggagttaag
      421 gcgcaaagtg ggaactcggt gggtggagga tccgcaaccg ggagcggtgg aagcgtatga
      481 attgaatcga aggcttgtgg aagggaagcg ggtacgatta gaattggatg tcgagcagcg
      541 tgatcgcttc catcgcctct tagcctacgt ctttgtaggc gatctttttg taaatgccga
      601 attggtcaag gctggttacg ccacggtctt cacctaccca cccaatgtcc gatatcacca
      661 actatttctc gacctccaaa aaaaagcgag ggttgagcat cgaggcctgt gggccaagcg
      721 aatgaaacag gcggctcaac ccataggtct cttagttgga tagaaggatt acaggcgctg
      781 atcatgaagc gggagcagcg aaagaagaag gaccgtcggg acgagtcgga agaccgtcgt
      841 gcgaaagagc ggcggaaggc ggagcggcga agccatgatc gccgggaggg gaaccggcgt
      901 ctcgagatct gtcctacttg cggccgcgtg ttgactcctt ctggttactg ctcgaattgt
      961 aaggtccgtg taatcaagat tcgaaattcg gcatagcgcg cgtcaaaaat tttatgagcg
     1021 agcgaaaggg agtttttcag tgtccactca ttctgccgtt gctttttcta gtatcggctt
     1081 gcgccacggt tcaaagaccg gcggaaccgg aaatcggcta ccgggaagta gggatcgctt
     1141 cttggtatgg taaggagttt catgggagaa ggactgccaa cggagagatc tatgacatga
     1201 acggcctgag cgctgcccat cggactctcc ctctcggtac cattgtgaat gtcaccaatt
//
LOCUS       DATMKH010000101         1933 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2937611, whole genome shotgun sequence.
ACCESSION   DATMKH010000101 DATMKH010000000
VERSION     DATMKH010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2937611"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1106)
                     /gene="gltX"
                     /locus_tag="VNV63_01245"
     CDS             complement(<1..1106)
                     /gene="gltX"
                     /locus_tag="VNV63_01245"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HXC61273.1"
                     /translation="MSSVRVRFAPSPTGYLHIGGVRTALFNWLFARHHGGVFILRIED
                     TDRSRSTEEAIQVIEEGMRWLGLNWDEGPYRQTDRLDKYREHADILFKEGKAYWCDCS
                     SEQLEARRAEALRKGLPPKYDGRCRDRGLASGPNRALRFRTPQTGQTVVEDLVKGTVV
                     FDNIQLDDLILVRADGFPTYNFCVVLDDADMKITHVIRGDDHLNNTPRQLHLYHAFGY
                     SPPKFAHLSMILGPDKARLSKRHGATSVLAYREMGYLPEALVNYLSRLSWSSGDQEIF
                     SLQELVEKFSLEVVGKSAAVFNPDKLLWLNAHYIHTGDPDRLIALLKPFLALEGLIKD
                     VGAIHELPLHDAAWLKKMGLALKERSRTMVEMAHS"
     gene            1202..1720
                     /gene="atpF"
                     /locus_tag="VNV63_01250"
     CDS             1202..1720
                     /gene="atpF"
                     /locus_tag="VNV63_01250"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HXC61274.1"
                     /translation="MPQFDTHFLTPLIFWSLISFGLLLGLLSKFVLPPLIAGLEERKK
                     TIKGNLEQAEQMKKDAEQLLIQYREKLKRAAEEAEKILAETQQSAQRLLEENQQRIGQ
                     ETERMIAEARQEILREQQKAMNELKRYVVNLTLLVTEKVLRRTLNDSDNLRLIEESVE
                     EFAAQDKTRLNR"
     gene            complement(1746..>1933)
                     /locus_tag="VNV63_01255"
     CDS             complement(1746..>1933)
                     /locus_tag="VNV63_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61275.1"
                     /translation="RLAVEYILLWEATDLGNLPVRIVYTGPDYITKIVEYRNVRIEPV
                     DASLFQPPKGFPSMSPF"
BASE COUNT          458 a          513 c          542 g          420 t
ORIGIN      
        1 gaatgagcca tctcgaccat tgtgcgtgag cgctctttga gcgccaggcc catttttttt
       61 aaccacgccg catcgtgtag gggcaattca tgaatcgccc ctacatcttt gatcaagcct
      121 tccagcgcca gaaagggttt cagcaaagcg atcaggcgat ccggatcgcc cgtgtgtatg
      181 taatgcgcat tcagccagag gagcttgtcc gggttaaaca ccgccgccga ttttccgact
      241 acctccagtg aaaacttttc gaccaattcc tgaaggctga agatctcctg atcgccggat
      301 gaccagctca agcgggagag ataattcacc agggcctccg gcagatagcc catttcccgg
      361 taggccagga ccgaggtggc gccgtgacgt ttcgagagcc gggctttgtc cggcccaagt
      421 atcattgaga gatgggcaaa cttcggcggg ctgtatccga aggcgtgata gagatgcagc
      481 tggcgcgggg tgttattcag atgatcatcg ccgcggatca catgcgtgat cttcatgtcg
      541 gcatcgtcca gcacgacgca gaaattgtaa gttggaaatc cgtcggcccg taccagaatc
      601 agatcatcca actggatgtt gtcaaaaacc acggtgccct tgacgaggtc ttctaccaca
      661 gtctgccctg tttgaggggt cctgaagcgc agcgcacgat tgggaccgga tgcgagcccg
      721 cgatcacggc agcggccgtc atatttcggg ggcagtcctt ttctgagagc ctcggcccgc
      781 cgggcttcga gctgttcgga ggagcagtcg caccagtaag cctttccttc tttgaataga
      841 atatccgcat gctcacggta cttgtccagc cgatccgttt ggcgataagg tccctcatcc
      901 cagtttagcc ccagccatcg cattccctcc tcgattacct ggatcgcttc ctcggtagag
      961 cgggaacggt cggtgtcttc gatccggagg atgaagacgc cgccgtggtg ccgtgcaaag
     1021 agccagttga acagcgcggt gcggacgccg ccgatgtgga gataaccggt agggctggga
     1081 gcaaatctga cgcggacgga cgacatagga gccaaattat gccatgaccc tctcaaaaag
     1141 taaaggttcg ttgaggtttt ccttgacagg accgcccgga ttcgttatag aatgatgcgc
     1201 catgcctcag tttgacaccc attttcttac gccgcttatt ttttggtccc ttatctcgtt
     1261 cggtctcctc ttgggtcttc tctccaagtt tgtccttccg ccgcttatcg cggggctcga
     1321 ggagcgtaag aagacgatta agggaaatct cgagcaggct gagcaaatga agaaggatgc
     1381 ggagcaactc ttaattcagt acagggaaaa gctcaaacgg gccgcagagg aagccgaaaa
     1441 aatcctggca gagacacaac agagcgcaca gcggttgctg gaagagaatc agcagcgcat
     1501 cggccaggaa accgaacgga tgattgctga ggcgagacag gagatacttc gagaacagca
     1561 gaaggcaatg aacgagttaa aacgttatgt ggtgaatctc accctcctcg ttacggagaa
     1621 ggtcctgagg cggaccttaa acgactccga taacctccgc ctcatcgaag agtcggtcga
     1681 agaatttgct gcgcaagata agacccgctt gaaccgctag attaaatctc gatagttgca
     1741 gacggctaga acgggctcat gcttgggaaa cctttgggcg gttgaaacag agaggcatcc
     1801 accggttcga tccggacatt ccggtactcg acaatctttg tgatatagtc cggacccgta
     1861 tagacgatgc ggacaggtaa atttccaaga tcggttgcct cccagaggag gatgtactca
     1921 accgcaagcc gct
//
LOCUS       DATMKH010000102         2053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3258264, whole genome shotgun sequence.
ACCESSION   DATMKH010000102 DATMKH010000000
VERSION     DATMKH010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3258264"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="VNV63_01260"
     CDS             <1..392
                     /locus_tag="VNV63_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004434061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HXC61276.1"
                     /translation="VAVDDCGNIINPMIVEGQIHGGLTEGLAIAFMQEIAYDESGNVQ
                     GGTFMDYLVPTAVETPTWETGKTCTPSPHHPFGAKGVGESPNVGSPAAFVNAVVDALS
                     HLGVKHIDMPIAPWKIWKILKEKGISE"
     gene            396..998
                     /locus_tag="VNV63_01265"
     CDS             396..998
                     /locus_tag="VNV63_01265"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HXC61277.1"
                     /translation="MAIRIEKTFQIGEPLERVWKFISDPRKVANCLPGAQITETVDES
                     TFKGLIEVQVGPSVTDYKGQVRIERLDEQNHEIEIVGKGQDVRGKGSASMKMTGKVQS
                     LPDGSTEVASVAEVNVVGLLAQMGARMIQEVSNKMFAEFTSNLRARLEQERGPASEPA
                     KTQITTEEPQPIKALPIVLSIARESAGRVFRRIFKGSDFL"
     gene            1013..1906
                     /locus_tag="VNV63_01270"
     CDS             1013..1906
                     /locus_tag="VNV63_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HXC61278.1"
                     /translation="MARQFRDIEDVIERLGRLGYVADRALATAIYLLLNLGKPLLIEG
                     PAGVGKTEAAKALAAMLETELIRLQCYEGLDLNSAVYEWNYQKQLLAIKIQEGSDKTV
                     EEKENYVFGSNFLLKRPLLQSILSEERAPVLLVDEIDRADEAFEAFLLELLAEFQITI
                     PELGTIQAKRVPHVILTSNSSRELSDALKRRCLYHWIDYPDLAKELEIVRVRLPEIAV
                     DLSSEVVRFIHTIRDLNLSKPPGVSETIDLSRSLILLEKTKLDQEAAEETLGCILKSA
                     DDLTKVRSEGLSRLLSRSGGS"
BASE COUNT          511 a          495 c          599 g          448 t
ORIGIN      
        1 ttgtcgccgt agatgattgc ggaaatatta tcaatccgat gatcgtggaa gggcagatcc
       61 atggcggcct gaccgaaggc ttggcgattg ccttcatgca ggagattgcc tacgacgaaa
      121 gtgggaatgt tcagggcggt actttcatgg attacctggt tccgactgcc gtagagactc
      181 caacctggga gaccggcaag acgtgtacgc cgtcgcccca tcatccgttt ggagccaagg
      241 gcgttggtga atcaccgaac gtcggatcac ccgccgcctt tgtgaatgcg gtcgtggatg
      301 ccctgtcgca tttgggagtc aagcacatcg acatgccgat tgcgccttgg aagatttgga
      361 agatcctgaa ggagaaaggg atttcggaat aggatatggc aatcaggatt gagaagacct
      421 ttcaaatcgg agagccatta gagagggtct ggaaattcat cagtgacccg agaaaagtcg
      481 cgaactgtct ccccggtgcg cagattacag aaacggtgga tgagagcacc ttcaaaggtc
      541 tgatcgaggt tcaggtcggg ccgagcgtca cagactacaa aggccaggta cgtattgagc
      601 gtctcgatga gcagaaccac gagatcgaaa tcgtcggcaa aggtcaggat gtgcgtggca
      661 agggcagcgc gtccatgaag atgaccggaa aggtccaatc tctgccggac ggcagcaccg
      721 aagttgcgag cgtggcagag gtcaacgtgg tcggactgct ggcgcagatg ggcgcccgca
      781 tgatccagga ggtttcaaat aagatgttcg cggaattcac ctcgaatctt cgcgcccgac
      841 tcgagcagga gaggggtcca gcgagtgaac cggccaagac acaaatcacc acggaagaac
      901 ctcaaccaat caaggctttg ccgattgtcc tgtcgattgc tcgggaatcc gcgggccggg
      961 tctttcgacg aatttttaag ggctcggatt ttttgtagct cacctggaga tcatggcccg
     1021 ccagttccgt gatattgaag acgtgattga acgactcgga cgtcttggat acgtcgcgga
     1081 tcgggcgttg gcgactgcaa tttatctact cctgaatctt ggcaaaccgc tcctgatcga
     1141 agggcccgcg ggcgtaggaa aaaccgaggc tgcaaaggca ctcgcagcca tgctcgagac
     1201 agaactgatc cggctccagt gctacgaagg attggatctg aattcagctg tctatgagtg
     1261 gaactatcag aagcaactgc tcgccatcaa gattcaggaa ggcagcgaca aaacggttga
     1321 ggaaaaggag aattacgtgt tcggatcgaa tttccttctc aagcgccctc tcttgcaatc
     1381 gattctgtct gaagaaagag cgcctgtttt gctggtggat gaaattgatc gagcagacga
     1441 ggcattcgaa gcgtttctgc tcgagctact cgcggagttt cagattacca tccctgagct
     1501 ggggacgatt caggcaaaga gagtaccgca tgttattctc acctcgaaca gcagccggga
     1561 actgagcgat gcgctcaaaa gacggtgtct ttatcattgg attgactatc cagacctggc
     1621 caaagaattg gaaattgttc gggtcaggct accggagatc gcagtggatc taagttcgga
     1681 agttgtccga tttattcata cgatcagaga cctgaacctc tccaaacctc cgggggtttc
     1741 cgaaacgatt gacctgtctc gatcactgat tttgctcgag aaaaccaaac tggatcagga
     1801 agccgcagaa gaaacgttgg gatgtatttt gaagtctgcc gacgatctta ccaaggtcag
     1861 gagcgaaggc cttagcaggc tcctctccag gtccggaggt agctagtgtc tcgtccccgc
     1921 actaaatcca tcgttgaatt ctgccggttt gcgcgggcaa atggagtgag cgctggaacg
     1981 aaggagacga tcgattgtct ccaagttgcg gaagccatcg cagatgccga tcgggatacg
     2041 ttcagattcg cct
//
LOCUS       DATMKH010000103         1090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3577206, whole genome shotgun sequence.
ACCESSION   DATMKH010000103 DATMKH010000000
VERSION     DATMKH010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3577206"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="VNV63_01275"
     CDS             <1..356
                     /locus_tag="VNV63_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015402734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61279.1"
                     /translation="GATGQASLLDFRLLAHSVSRLEGGVYLNIGSAVLLPEVFLKAVT
                     LARNLGHRLNRFTTVNMDFIQHYRPITNVVKRPTQQGGKGYMLTGHHEIMVPLLAAGI
                     VEALEQNKKRRSYSP"
     gene            353..844
                     /gene="rfaE2"
                     /locus_tag="VNV63_01280"
     CDS             353..844
                     /gene="rfaE2"
                     /locus_tag="VNV63_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HXC61280.1"
                     /translation="MNHKVKNLPALLQIIRRYKKQGKRIVFTNGCFDLLHLGHVTYLE
                     RAKSKGDLLIVGINSDRSMRTLKGRERPLVPQSERGRVLAGLSSVDYVTIFDEPTPLS
                     LICALEPDVLVKGADWKKDSIVGGGEVKHRGGKVITIPVVKGISTTQIIKRIVERYAR
                     RKK"
     gene            889..>1090
                     /locus_tag="VNV63_01285"
     CDS             889..>1090
                     /locus_tag="VNV63_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61281.1"
                     /translation="MNYPILSNRIFDPVYQAVKAGVRPITVAGLFGSAMAFFLASLHH
                     ALLRPLLLLTATPDEADRLAEDI"
BASE COUNT          250 a          298 c          301 g          241 t
ORIGIN      
        1 gcggtgcgac gggacaagca tcgcttctgg attttcgtct tctggctcat tcggtcagcc
       61 ggctggaagg cggggtttat ctcaacatcg gatcggcggt attactgccg gaggtgttcc
      121 tcaaggccgt aacgttggcg cgcaatctcg gccatcgtct gaaccggttt acgaccgtga
      181 acatggattt tatccagcat taccgcccga tcacgaatgt ggtgaagcgg ccgacacagc
      241 agggcggcaa aggttacatg ctcaccggac atcacgagat tatggttcct cttctggccg
      301 ccgggatcgt cgaggcgctt gagcagaata aaaaaagaag gtcctactct ccgtgaacca
      361 taaggtcaag aacctccctg ccctcctcca gatcatccgg cgatataaga aacagggaaa
      421 gcggatcgta tttaccaacg gttgctttga tcttctccac ctgggtcatg taacctacct
      481 ggagcgggca aaatccaagg gagatcttct aattgtaggg atcaacagcg atcgctccat
      541 gcgtacactc aagggaagag aacgaccgct cgtcccgcaa tcggagcgcg gtcgggttct
      601 ggccggattg tcatcggtag actatgtcac aattttcgat gagcctaccc cgctttcgct
      661 gatatgtgct ctcgagccgg atgtgctggt taaaggagcg gactggaaaa aagacagcat
      721 tgtcggcggc ggcgaggtca agcatcgggg cggaaaggtc atcacgattc cggtcgtcaa
      781 aggcatctcc accacgcaga tcatcaaacg gattgtagag cgatacgcta gaaggaaaaa
      841 gtgatataat taggcttagg cggatctgct caaacatatc ttctgcagat gaattatccg
      901 attctatcaa accggatttt cgaccccgtg tatcaggcgg tcaaggccgg agtccggccg
      961 atcacggttg ccggcctttt tgggtcggcg atggccttct tcctggccag cctgcaccat
     1021 gcgctcctcc gacctctgct cctcctgacc gcaaccccgg acgaggcgga tcgcctcgcc
     1081 gaagatatcc
//
LOCUS       DATMKH010000104         1031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_52974,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000104 DATMKH010000000
VERSION     DATMKH010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52974"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="VNV63_01290"
     CDS             <1..619
                     /locus_tag="VNV63_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61282.1"
                     /translation="AKNGDTKHPEVWFGAYLREHYSSGIEVSVISNHEWRKARRNILR
                     LLDADHDGVLTATERGQAKIILFGHSWGASEASAFARDLEQLGIPVMLTIQIDIVSKP
                     GQNPILIPPNVEEAINFFQSEGPLHGRKQVVAEDPGRTEIIGNVHMTYEGHRVDCRNY
                     PWFARTFNKPHHEIENDARVWSQIALIIDSRLSSLRSPIDQAAMH"
BASE COUNT          260 a          269 c          271 g          231 t
ORIGIN      
        1 cgcaaagaat ggggacacca aacaccccga ggtctggttt ggtgcctatc ttcgggaaca
       61 ttattcgtcg gggattgaag tctcggttat ttcgaaccac gagtggcgaa aagcacggag
      121 aaacatctta cggctgcttg atgctgatca tgatggtgtt ctaacggcca cggaaagggg
      181 tcaggcaaag atcatcttgt ttgggcatag ctggggagca tcggaggctt cggcctttgc
      241 cagggacctg gagcagcttg ggattcccgt gatgctcacc attcaaatcg atatcgtatc
      301 caaacctggt caaaatccga tcctcatacc tcccaatgtt gaagaggcaa tcaatttctt
      361 ccaatcggag gggcctctcc acggacgaaa acaggtcgtc gccgaagatc cgggacggac
      421 agagattatc ggcaatgttc atatgactta tgaaggtcac cgagtcgatt gccggaacta
      481 cccttggttc gcccgcacgt tcaacaagcc ccatcatgag atcgagaacg acgcccgcgt
      541 gtggagccag attgcactga tcattgattc gcgtctttcc tcgttgagaa gcccaatcga
      601 ccaggctgca atgcactgac gtcgattcac aaatacgaga ggattctgtc ctcgtaaaag
      661 gctgggccac cgccgcctcg ggatcgaacc cggactgcga acacgtgtgc gatcagcaag
      721 ggtgagcatc aggctttcat catcggcagg gcaacaaatc tcactgacgt gacgttacag
      781 gatatttcgg aaggtcccga gaacactttg ccaaaccaac ttcgtgcagg attgaaaaga
      841 tgcagagtac agcttgccga catctcaacc ccgaccgggg ctcatttccg agcctcaggt
      901 tgcaggcgct ccttcgttgt gtgctagatt cttggatcga aattcgtaaa caagaatagt
      961 tcaacaaaca ggcagtaaca tcaggccgct cttaacaggt caacgatgtt ctcgatggcc
     1021 ttgtcggcct t
//
LOCUS       DATMKH010000105         1287 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4532659, whole genome shotgun sequence.
ACCESSION   DATMKH010000105 DATMKH010000000
VERSION     DATMKH010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4532659"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="VNV63_01295"
     CDS             <1..488
                     /locus_tag="VNV63_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61283.1"
                     /translation="GSAGTEPFPNLGLDLRDDHPVGMEIPTTDPQFSQIMGNLFLLDG
                     GGTGHTPGSNKVFWITRTGFLQPDKRDRVRAYPSNPNRLDAPYIECASCHNPHEASRP
                     KDANHPGGQPALTDPINVVTVNNSLFLRAPSFPDPGTFPNSLLQDRNQGSLLCLSCHK
                     K"
     gene            575..>1287
                     /locus_tag="VNV63_01300"
     CDS             575..>1287
                     /locus_tag="VNV63_01300"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HXC61284.1"
                     /translation="MPYEVLSQVVHPQDTQEPLSSVNALGGQGVVATVNQKPITSQEL
                     DEAVNSSLPRAYGHRQLSETRIVEIKKEVLEDLIQKELLYQEAKRENIRVSPQEIEAA
                     VAKIRKRFSSEKEFMAALKKSDLTLEKVRLGTEKFLTVSKVMSMAVDSKVVIQEQDLV
                     NYYETNRQRFVLPEEREIRQILIAVDPGASDKGWEAGLKKANEIFKKIKAGQDFAELA
                     KIGSDDKSTRGQGGSLGFLP"
BASE COUNT          322 a          299 c          374 g          292 t
ORIGIN      
        1 atggctctgc tggcaccgag ccgtttccca acctggggct ggatcttcgg gatgatcatc
       61 ccgtcgggat ggaaatcccg acgaccgatc cgcagttttc acagatcatg ggcaatcttt
      121 ttcttctgga cggcggcggt accgggcata cgccggggag caacaaggtc ttctggatca
      181 cgagaaccgg cttcttgcag ccggataagc gggatcgagt ccgggcctac ccgtctaatc
      241 cgaatcgtct ggacgctccc tatattgaat gtgcctcctg ccacaaccct cacgaggcca
      301 gtcgccccaa agatgccaat cacccagggg gtcagcctgc tctgaccgat cccattaatg
      361 tggtgaccgt taacaactcg ctttttttaa gggcgccctc gtttccagat cctggaacgt
      421 ttccgaattc tctcttacag gatagaaacc aggggagtct cctctgtctg agctgtcaca
      481 aaaagtgagg aagcgtgatg ctggaatcag ttggatggat gatgaggata gatcagcata
      541 ttttttgtgg tgtttggtag gagttctttg tctcatgcct tatgaagttt tgtctcaggt
      601 ggtgcaccca caggacactc aagagcctct atccagtgta aacgcgcttg gcggtcaagg
      661 tgtggtggcc accgtaaacc agaagccgat tacctctcaa gagttggacg aggcggtaaa
      721 cagctcgctt ccacgggcct atgggcatag acaactttct gaaacgcgga ttgtggagat
      781 taagaaggag gttctagagg atctcatcca gaaagagctg ctgtaccagg aggccaagcg
      841 ggagaacatt cgcgtgagtc cgcaggagat cgaggcggcg gtggcaaaaa tccggaaaag
      901 gttctcatcc gaaaaggaat tcatggcggc tctcaaaaag agcgacctga ctctggagaa
      961 ggttcgattg gggaccgaga agtttcttac ggtcagcaag gtgatgtcaa tggctgtgga
     1021 ttcgaaggtg gtgattcaag agcaggatct ggtcaattat tatgaaacga atcggcagag
     1081 atttgtcctt ccggaggaaa gagagatccg gcagatcttg attgcggtgg acccgggtgc
     1141 ttcggacaaa ggctgggagg cgggtttaaa aaaagcaaac gagattttca aaaaaataaa
     1201 agccggtcag gattttgccg aactggcaaa gatcggatca gacgataaat ctacaagggg
     1261 ccagggagga agtctgggct ttctccc
//
LOCUS       DATMKH010000106         1078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1654772, whole genome shotgun sequence.
ACCESSION   DATMKH010000106 DATMKH010000000
VERSION     DATMKH010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1654772"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..880
                     /locus_tag="VNV63_01305"
     CDS             <1..880
                     /locus_tag="VNV63_01305"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HXC61285.1"
                     /translation="AEGPSGGAFDLDKLKRMRDACDAVGMKIEGFRMDSGYIVMKPGP
                     ERDRKINQILENIRKTHLVGVGLISQHWTMIPIRRNGKEPGRGGSTYEAFKLEENWKD
                     LPPGIAGNVSSEEYWERITYFLQKVIPVCKEYNVKMANHPYDPPGLPFGYEHVENFDS
                     PSIFAAYKRYEMIVDSPYNGFQLDLGVLAEGSTDANKQIPPLVRYLGERGKIHQIHMR
                     AIKGGLNHFAEVYPDEGVLDLFKIMRILRDTGYEGGILPDHMPSHPGDPGKLQAYAFG
                     YGYIQALINGVNSEVS"
     gene            complement(852..>1078)
                     /locus_tag="VNV63_01310"
     CDS             complement(852..>1078)
                     /locus_tag="VNV63_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61286.1"
                     /translation="QVAAIVQALQRNPPAYIALFPVKPEYALHGDNAAPFRQFVSNNY
                     ERANVFSQNQSHEEELWQRSPAMTPLNSRH"
BASE COUNT          267 a          281 c          310 g          220 t
ORIGIN      
        1 tgcggaaggc ccctcgggag gcgcctttga tctcgacaag ttaaagcgga tgagggatgc
       61 ctgcgatgcc gtgggaatga agatcgaagg cttccgtatg gattccggtt acatcgtgat
      121 gaagccgggt ccggagcgcg atcgaaagat caaccagatc ctcgaaaata tccgcaagac
      181 gcacctcgtg ggagttggcc tcatcagcca gcattggaca atgatcccca tccgtcgaaa
      241 cggaaaagaa ccgggcagag gaggatcgac ctatgaagca ttcaagctgg aagagaattg
      301 gaaagatttg ccgccgggaa ttgccggaaa cgtcagctcg gaagagtact gggaacgaat
      361 cacttatttt cttcagaaag tgattcctgt ctgcaaggag tacaacgtca agatggccaa
      421 ccatccctac gatcctcccg ggctgccgtt tggctacgag cacgtggaga acttcgactc
      481 cccgtcgatt ttcgcagcgt acaaacggta cgagatgatc gtcgacagtc cctacaacgg
      541 ctttcagctg gatctgggag tactcgccga agggtccaca gacgcgaaca agcagatccc
      601 tcctctcgtg cgctatcttg gcgaacgcgg caagatccat cagattcaca tgcgcgcgat
      661 caagggcgga ctcaatcact ttgcggaagt ctatcccgat gagggggttc tcgacctgtt
      721 caagatcatg cggatactga gggacacagg atacgagggc ggcatcttac cggaccacat
      781 gccttcccat ccgggcgatc cgggaaaact gcaggcctat gcgtttggat acggctatat
      841 ccaggctctc atcaatggcg tgaattcaga ggtgtcatag ccggggaacg ttgccagagc
      901 tcttcctcat gggactgatt ctgggaaaag acattcgccc gctcgtagtt gttggaaacg
      961 aattgacgaa aaggtgcggc attgtccccg tgcaaagcgt actccggctt aaccggaaac
     1021 aaggcgatat aggccggcgg attgcgttgc aacgcttgca cgatggcggc cacctgtt
//
LOCUS       DATMKH010000107         3888 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1655068, whole genome shotgun sequence.
ACCESSION   DATMKH010000107 DATMKH010000000
VERSION     DATMKH010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3888
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1655068"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..632
                     /locus_tag="VNV63_01315"
     CDS             72..632
                     /locus_tag="VNV63_01315"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HXC61287.1"
                     /translation="MDQEIAKGKFLIALPILRDPNFRQTIVLLCEHGPDGSLGLVVNR
                     PTGVEVSTLINDLPDLAGAGLMHNGGPVDKNGMLILCHGEASSENHGILKDVFLITDL
                     AVLTVLKKTGGLEPNGRIRCYLGYAGWAPGQLEAEMRAGAWRLVAGDSRLIFNADPAA
                     VWQETMHQLGGEWAIYALMPPDLNSN"
     gene            788..1834
                     /locus_tag="VNV63_01320"
     CDS             788..1834
                     /locus_tag="VNV63_01320"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HXC61288.1"
                     /translation="MTKRVTVIGGGSWGTSLAFLLADKGYPVKLWLYEKDLILQIQRD
                     RENSLYLPGIKLPYSITATSSFEEAVAGSEMILFVVPSHVTRSVLIQLAPYLPAGVPI
                     VCATKGIENDTLMLMSDVMKDVLPAEHHARFAFLSGPSFAKEVCQRLPTAVSLAAADH
                     DLATKLQETFATPYFKVFTTTDVIGVQVGGALKNVIALAAGGADGMGFGTNTRAALIT
                     RGLLEIMRLGVALGAQSRTFYGLSGIGDLVLTCTGELSRNRTVGFKIGQGMKVREILA
                     EMKMVAEGVNTAKSAYQLAQKVQVKMPIVEQIYAVLFHDKNPREAVTDLMEQAAGDEM
                     DAADLDREGKGPRG"
     gene            1837..3450
                     /locus_tag="VNV63_01325"
     CDS             1837..3450
                     /locus_tag="VNV63_01325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HXC61289.1"
                     /translation="MITTIFLSLLGGTCLLLYGLQLSGQGLQGVAGTRLRTIINAVTQ
                     HRFLGVGAGAVVTTLFQSSSATTVMLVGFASAGLITLDQTIALILGADIGTTLTVQLL
                     AFQIFSGSLLLIGIGFLLSFLGKQPGTKSLGQAILGFGMIFLALKILTDGTEPLKTSE
                     LARQLLSGFAEEPVWGMVFAAIFTAMTTSSTATIGIALAFGHQGLLPLSGAIPIILGA
                     NIGTCATALISSLGAKAEAKRVAWAHTLFKVIGVLLIYPFLKPFEALVVHTAQDPLRQ
                     IANAHTLFNLGIAILFLPFASPLARLVTRMIPEKPMGEDPSQPKYLDPHVLDSPSLAL
                     GQATREALRMSDIVQEMLIETIRVFKENNQSLLEGIEKSENWVDQLNRQIKLYLTKLS
                     EHVLTAEQSQREFALLTMINDLENIGDIIDKNLLDLARKKIYKGLRFSDKGLEEIVEL
                     HQLVLKNFQLVISAFTAQDVTLAQQVIKEKIAIHQKEREFKQAHIHRLHQGLPETIET
                     SAIHLDVLTSLKRINSHVTGMAYPIVEAK"
     gene            3447..>3888
                     /locus_tag="VNV63_01330"
     CDS             3447..>3888
                     /locus_tag="VNV63_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-amino-4-imidazole-
                     carboxylate carboxylase"
                     /protein_id="HXC61290.1"
                     /translation="MNEQRLKKLLSSLQSRKISVEEALERLKILPYEDLGFASVDHHR
                     LLRQGLAEVVYCEGKTKTQIVEIIRRIRQTDTPILATRADPAIYKAVHRLDKRARYHE
                     AARTIVIQDREKHLTGNILILTAGTSDIPVAEEASVTAETLGSRV"
BASE COUNT          919 a         1042 c         1063 g          864 t
ORIGIN      
        1 aaaatttacc ttgccattaa cgaaatagtt gattaagatg gagaaatgat ttgtgccata
       61 tcgggagtct gatggaccag gaaattgcaa aaggaaaatt tctgatcgct ctgccgatcc
      121 taagagaccc taattttcga cagacgattg tcttgctctg cgagcatggt cctgacggat
      181 cgctgggact ggtggtaaac cgtccgaccg gtgtagaggt ctcaacattg atcaacgact
      241 taccggacct ggctggcgcc ggtctcatgc acaatggagg cccggtggat aaaaacggga
      301 tgctgatcct ctgccacggc gaagcctcat ctgaaaacca tggcatccta aaagacgttt
      361 tccttatcac ggatcttgct gtactaactg tattaaaaaa aaccgggggg ttggaaccaa
      421 acggaaggat tcgttgctat ctcggttatg ccggctgggc accaggccaa cttgaagccg
      481 aaatgcgggc gggcgcttgg cgtcttgtcg cgggcgactc ccgattgatc tttaacgctg
      541 accccgctgc tgtctggcaa gaaacgatgc atcagttggg tggggagtgg gcgatctatg
      601 ccttgatgcc tccagacctg aactctaact gatttcacga cacgccgcta tacttgactt
      661 gtttcttttt aaaacgctat aatcccttcg cctcgcaatc tcaagacgga aaattgaaga
      721 gtcccgccct aaagggcagc gtgcgctctc gatgcgcgtt caggccagac cggcagaagg
      781 agtccatttg acaaagcggg tcacggtaat cggcggtgga agttggggaa caagcctagc
      841 cttcctgttg gccgataaag gctacccagt caagctctgg ctttatgaaa aagatttgat
      901 cctgcagatc caacgggatc gggaaaacag tctctatctt cccggcatca agctccccta
      961 ctcgattacg gcaacgagtt cgtttgaaga ggcggtcgca ggcagcgaaa tgatcctgtt
     1021 tgtagtcccg tcccatgtca cccggtctgt gctcatccag ttagcccctt atctacccgc
     1081 cggcgttccg atcgtgtgtg ctacgaaggg gatcgagaat gacacgctca tgctgatgtc
     1141 ggatgttatg aaggatgtcc tccccgctga acatcacgcg cgatttgcat ttctttccgg
     1201 tcccagcttt gccaaggagg tgtgtcaacg cctcccgacc gccgtttcgc tcgcggctgc
     1261 agatcatgat ttagcgacca aactccagga gacctttgcg acgccgtatt ttaaggtctt
     1321 caccacaacg gatgtgatcg gtgttcaagt cggcggagcg ttgaaaaacg taatcgccct
     1381 ggcggcggga ggagcggatg gaatgggttt cggcacgaat acccgagcgg ctttgatcac
     1441 acgaggactt cttgaaatca tgcggctggg tgttgcgtta ggtgcgcagt ccagaacctt
     1501 ctacggcctt tccggaatag gcgatctggt cttgacctgc acgggtgagc tcagccggaa
     1561 tcgtaccgta ggctttaaga tcggacaggg gatgaaggtc cgagagattt tggccgagat
     1621 gaagatggtc gcggaagggg tcaacaccgc taaatccgct tatcagctcg cacaaaaagt
     1681 tcaggtcaag atgccaatcg tagaacagat ctatgcggtt ctgtttcatg ataaaaatcc
     1741 ccgcgaagcg gtgaccgacc tgatggaaca ggccgcgggg gatgagatgg atgccgccga
     1801 cctcgatcgg gaaggaaaag gcccgagagg gtaaaattga tcacgacgat cttcttgagc
     1861 ctcctcggag gaacctgtct cctgctttac ggcctccagt tatctggaca gggacttcaa
     1921 ggagtggcgg gcacgcgtct ccgaacaatc atcaatgcgg taacgcaaca ccgattcctg
     1981 ggtgtaggcg ccggggcagt tgtcacgacg ctgtttcaga gcagcagcgc cacgaccgtc
     2041 atgttggtcg gttttgccag tgccggtcta ataacattgg atcagaccat cgccctgata
     2101 ctcggagcgg acatcgggac caccttaacg gttcagctcc tcgcctttca gatcttcagt
     2161 ggatcgctgc tcctgatcgg aatcgggttt ctcttatcat tcctgggcaa gcaacccgga
     2221 accaagtcgc tgggtcaggc gatcttaggc ttcgggatga tcttcctcgc cttaaaaatc
     2281 ctgacggatg gaaccgagcc gcttaaaacc agcgagttgg cccggcaact actatccggc
     2341 ttcgcagagg agccggtttg gggaatggtt tttgcggcga tcttcacggc gatgaccacg
     2401 agcagcacgg cgacgatcgg gattgcgctg gcctttgggc atcaagggct gctcccactc
     2461 tcgggtgcga tcccgattat cctgggcgcg aatatcggca cctgtgccac ggccctgatc
     2521 tccagcctgg gagcaaaagc agaagccaag cgggtggcct gggcccacac gctatttaag
     2581 gttattggag tgctcctaat ctatccattc ctgaaaccgt ttgaggccct tgtggtccat
     2641 accgctcaag acccgcttcg tcagatcgca aacgcgcaca cattgtttaa tctcggcatc
     2701 gcgatcttgt tcctaccgtt cgcttcgccc ttggcccgtc tcgtgacgag gatgattccg
     2761 gagaaaccga tgggtgaaga tccgtcccaa cccaagtacc tggatcctca tgtcctcgac
     2821 tccccttcgc tcgccctggg tcaggcgaca cgggaggcct tgcggatgtc ggacattgtc
     2881 caggagatgt tgatcgagac gatcagggtc ttcaaagaaa acaaccagtc cctcctggag
     2941 ggcatcgaga agtcagaaaa ttgggtggat caattgaacc ggcagatcaa gctctacctt
     3001 acaaagctgt ccgaacatgt tctgaccgcg gagcaatccc agcgggagtt tgcccttctg
     3061 acaatgatca atgacctgga aaatatcggc gacatcatcg ataaaaacct tctggacttg
     3121 gcccgaaaaa agatctacaa aggtcttcgc ttttcggaca agggactcga agaaattgtc
     3181 gaactgcatc agcttgtctt gaaaaatttc caactggtga tctcggcctt caccgcccag
     3241 gatgtcacac tggctcaaca ggtgatcaaa gagaagatcg ccatccatca gaaagaacgc
     3301 gagttcaagc aagcccacat tcaccggctc catcagggtc ttccagaaac tatcgagacc
     3361 agcgcgattc acctggatgt tctgaccagc ctcaagcgga ttaattccca cgtcaccggc
     3421 atggcctatc cgattgttga ggcaaaatga atgaacagcg gttaaagaaa ctcttgagtt
     3481 ctcttcagtc caggaagatt tctgtcgagg aagctctgga gcggctgaag atcctgccgt
     3541 acgaagacct cggcttcgcc tcagtggatc atcaccgctt gcttcgtcag ggtctggccg
     3601 aggtggtcta ctgtgaagga aagacaaaga cgcagatcgt tgagatcatc cggcgcatcc
     3661 gacagacgga tacaccgatc ctcgcgacgc gggccgatcc ggccatctat aaagcggttc
     3721 accgtctcga caaacgcgcc aggtatcatg aggcggcgcg gaccatcgtg atccaagaca
     3781 gagagaagca tctcacgggc aacatactga tcttaaccgc agggacatcc gatatcccgg
     3841 ttgccgaaga ggcaagtgtg accgccgaga cgctcggaag ccgggtca
//
LOCUS       DATMKH010000108         1100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1016692, whole genome shotgun sequence.
ACCESSION   DATMKH010000108 DATMKH010000000
VERSION     DATMKH010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1016692"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..873)
                     /locus_tag="VNV63_01335"
     CDS             complement(<1..873)
                     /locus_tag="VNV63_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015183961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HXC61291.1"
                     /translation="MKFAFYPGCASQGACPELYQSTQLVVQKLGIELVELRAASCCGA
                     GVIGEADPDLAFSINARTFSMAEEKGLDVLTICGTCQGVMGSANRQLKDDDALLARIN
                     GMLIKEGVRPYQGQIEVRHLLWIIVRNYGLEELKKLIKVRLNGLKVAPFYGCYILRPS
                     QMLGFDDAENPTSLEELIRALGGEPVEYSGRTKCCGFPIILEKEEIATAMVGNHTYEA
                     KQKGSDCMVTPCPLCHMNLDIYQERAEERIGADINLPILHLPQLIGLAMGFEPKALGL
                     SHHLVSTGKLTDKIS"
     gene            complement(876..>1100)
                     /locus_tag="VNV63_01340"
     CDS             complement(876..>1100)
                     /locus_tag="VNV63_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HXC61292.1"
                     /translation="GARHITAFTEIVKHEGKLNEAIMPLMMTWTQPKRFLRIIPLGLR
                     MFLKGKVPFPFKRPIAGIKQLRGLFKQRGK"
BASE COUNT          226 a          296 c          311 g          267 t
ORIGIN      
        1 actgattttg tccgtgagct tgccggttga gacaaggtga tgggaaagcc ccagcgcctt
       61 gggctcgaag cccatcgcga ggccgatcaa ttgaggaaga tgcagtatgg gcaggttgat
      121 atcggcgccg atacgctctt cggcccgttc ctgataaatg tccaggttca tgtggcagag
      181 cgggcaaggg gtgaccatgc agtcggagcc tttttgcttt gcttcgtagg tgtgatttcc
      241 caccatggcg gtagcgatct cttctttttc cagtatgatc ggaaagccgc agcatttcgt
      301 ccggccggag tattcgaccg gttccccgcc cagcgcccgg atcagttctt ccagcgaagt
      361 ggggttctcg gcgtcatcaa agccgagcat ctgagaagga cgaagtatgt aacagccgta
      421 gaacggtgcc accttgaggc cgttcagacg gactttgatc agttttttaa gttcttccag
      481 gccgtagttt ctcacgatga tccaaaggag atgccgcacc tcgatttggc cttgatacgg
      541 gcggactcct tctttaatca gcattccgtt gattctggcc agcagcgcat catcgtcctt
      601 aagctgccgg ttggcgcttc ccatgacgcc ctggcaggtg ccgcaaatcg tcaggacgtc
      661 gagacccttc tcctccgcca tcgagaaggt acgtgcatta atcgaaaacg caaggtccgg
      721 gtccgcctcg ccgatcaccc ctgctccgca gcaggaggcg gcgcgaagct cgacgagctc
      781 gatcccgagt ttttggacga ccagttgtgt ggactgataa agttcaggac aagcaccttg
      841 ggaggcgcat ccgggataga aggcgaactt catttttatt ttcctctctg cttgaacagg
      901 cctctcaact gtttgatccc ggcaattggc cgcttgaagg gaaacggcac ctttcccttc
      961 aaaaacatac gaaggcccaa ggggatgata cggaggaacc gcttgggttg tgtccaggtc
     1021 atcatgagcg gcatgatggc ttcattgagt tttccttcat gttttacgat ctcggtaaac
     1081 gcggtaatgt gccgggcccc
//
LOCUS       DATMKH010000109         1098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1336879, whole genome shotgun sequence.
ACCESSION   DATMKH010000109 DATMKH010000000
VERSION     DATMKH010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1336879"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="VNV63_01345"
     CDS             complement(<1..577)
                     /locus_tag="VNV63_01345"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXC61293.1"
                     /translation="MQKTEVPPKPAETVPEFSKASRQFRGGRALGLLFGTIVLFGAIA
                     LVSDTFLKPYTMFIIARQIAFSILIALSQAVCLVVGGMNLSVGAIGSMATVILGLCFQ
                     EVKLNAWLAVTITLLFGVVSGAINGLIITRLRINSFIVTLSLMFVYMGLRSGISGGSP
                     YTIPEDFGFIGQSAIFGVSWVFVLVCLILLAT"
     gene            complement(577..>1098)
                     /locus_tag="VNV63_01350"
     CDS             complement(577..>1098)
                     /locus_tag="VNV63_01350"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXC61294.1"
                     /translation="GYVTENRKEEGLFLGESVLTNLTLLVWKKMRHPVTRYISRKNEE
                     VAAGKMVSALSVRTRSLADLAANLSGGNQQKISIGRWLLADCDILIIDEPTVGVDVGA
                     KEQIHQLIWNLACIERKAIILISSEMAEIIRLSSRILLFADQQIIGEVSRLDDKSYTK
                     VRDEIGNLLSAIH"
BASE COUNT          282 a          295 c          270 g          251 t
ORIGIN      
        1 cggtggccag caagattaag cagacaagca caaaaaccca ggagactcca aagatcgcgc
       61 tctgcccgat gaatccgaaa tcttccggaa ttgtgtaagg agatccccct gaaatcccag
      121 agcgcaggcc catatagacg aacatcaacg acaaggtcac gatgaaggaa ttgatcctta
      181 ggcgggtgat aatcaatccg ttgattgcgc ccgacacaac accgaaaagt agtgtgatgg
      241 tgacggccag ccacgcgttc aatttaactt cttgaaagca gaggccaaga atcacggtgg
      301 ccatactacc aattgcgccg actgagagat tcatgcctcc cacaacaagg caaaccgctt
      361 gggacaaggc tatcaagatc gagaacgcga tctggcgcgc aatgataaac atcgtatagg
      421 gcttgaggaa tgtgtcggat actaaagcaa ttgccccaaa caatacgatg gttccaaaga
      481 gcaatccgag cgctcgaccg ccccggaact gcctggatgc tttactgaac tctggaactg
      541 tctcggccgg ctttggtggt acttctgttt tctgcattag tgtatcgcgc tcagaagatt
      601 tccaatttca tcgcgcactt ttgtatagga tttgtcatcc agacgactga cctcaccaat
      661 aatctgttga tcggcaaaaa gcaggatccg gctggacaaa cggatgattt cggccatctc
      721 ggaagagatc aggataatcg ctttccgctc aatacacgcc agattccaga tcaattgatg
      781 gatctgctct ttggcgccta cgtcgactcc cacagttggt tcgtcgatga tcaggatatc
      841 gcaatcggcc aatagccatc ttccgatgct gatcttttgc tggttgccac cgctgaggtt
      901 cgccgccagg tcggcgagac tacgtgtgcg cacggaaaga gcggagacca ttttcccggc
      961 ggcgacctcc tcattttttc tgctgatgta acgcgtcacc gggtgacgca ttttcttcca
     1021 gaccagcagc gtgagattgg tcagtacgga ctcaccgaga aacagccctt cttccttcct
     1081 gttctccgtt acgtagcc
//
LOCUS       DATMKH010000110         2014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1978344, whole genome shotgun sequence.
ACCESSION   DATMKH010000110 DATMKH010000000
VERSION     DATMKH010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1978344"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            184..600
                     /gene="nikR"
                     /locus_tag="VNV63_01355"
     CDS             184..600
                     /gene="nikR"
                     /locus_tag="VNV63_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HXC61295.1"
                     /translation="MKKTVRFGVSLDHHLLEKFDQMIQDKNYTNRSEALRDLIRDHLV
                     EQEWVENKEVMGTITLVYDHDVRELGEVLAHLQHRYNYLIRSTLHDHLDERNCLEVLV
                     VRGKGGQVKKIADRLIGTKGVKHGKLVMTTTGKGLA"
     gene            597..1565
                     /locus_tag="VNV63_01360"
     CDS             597..1565
                     /locus_tag="VNV63_01360"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HXC61296.1"
                     /translation="MRKKILILSVFYLFTLALPFESWASNSGKLKVLTTVSPLTNIAH
                     NIGGSLIELHGLIPEGTDSHTFEPAPSDVRFIAASDLIVLNGLHLEAPTERLVASNKK
                     PRAVVIKLGDQAIREKDWIYDFSFPKERGDPNPHLWLNVAHAMRYAELIRDALMQLDP
                     ENKPIYERNAKIYLKRLTQLDGAIKTAMQTIPAKNRKLVTYHDSFAYFCPRYGCTVIA
                     AIQPSDFSEPAPRDIAALIDQLKTEQIPAIFGSEVFPSKVMNQVGKEAGVKFVTTLRD
                     DDLPGPEGSPQHTYIGMIIEDVRTIVRSLGGNDDSLKEIKPEDRTS"
     gene            1575..>2014
                     /locus_tag="VNV63_01365"
     CDS             1575..>2014
                     /locus_tag="VNV63_01365"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXC61297.1"
                     /translation="MQPLVELENVTCGYDHHSIFEEVNLRIAQGQLAGIVGPTGSGKT
                     TLLKAILGMVKPMAGTIRVMGRSVSGQSIARIGYVPQLETVDWNFPVTVEQVVVMGRY
                     REMGWMPWPSRKDRHAVIGLLERLGMGAFVNRHIRELSGGQQQRV"
BASE COUNT          498 a          478 c          556 g          482 t
ORIGIN      
        1 tttgggattg cttacgtgga gaattccctg acctaatttg tgatgacaat gaagatcctc
       61 ctgctcgccc gacatccgtc cacctagctt gacttttgtt actgctggta ttattattta
      121 aaatatagta acacggaaaa aacacttaat tttttttgat gacatgccat tgagagaacg
      181 atcatgaaaa aaaccgttcg atttggcgtt tcactggatc atcacctcct ggagaagttt
      241 gaccagatga tccaggataa gaactacacc aaccgctcgg aagccctgcg ggatcttatc
      301 cgagatcatc ttgtggagca ggaatgggtt gaaaataaag aagtgatggg aaccatcacg
      361 cttgtctatg atcatgatgt cagggagctt ggcgaggtcc tggcccatct tcaacaccgg
      421 tataactatc ttatccgttc aacgcttcat gaccacctcg atgagcgaaa ttgtcttgag
      481 gtcctcgtgg tgaggggaaa gggtggacag gtcaaaaaga tcgctgatcg gttgatcgga
      541 acgaagggcg tcaagcatgg gaagcttgtg atgaccacga ccggtaaggg gttggcgtga
      601 gaaaaaagat cctcatctta agcgtattct atctgtttac acttgccctt ccatttgagt
      661 cctgggcatc aaattccggc aagctcaaag tactcacaac ggtctctccc ctcaccaaca
      721 tcgcacacaa tatcggaggg agtctgattg agctgcacgg cttgattccg gaggggacgg
      781 actcccacac ctttgagccg gcaccatccg atgtccggtt tattgccgca tcggatttga
      841 ttgttttaaa cggcctgcac ctggaggccc cgacggaaag attagtcgca tcaaacaaaa
      901 aaccccgggc cgtggtgatc aagttgggcg accaggcgat ccgtgagaaa gactggatct
      961 atgacttcag tttcccgaag gaacgaggcg atcctaaccc gcatctctgg ctcaatgtgg
     1021 cccatgcgat gcggtacgct gagctgattc gtgatgcgct gatgcagctt gatccggaaa
     1081 acaaaccgat ctatgagcgg aacgctaaga tctatcttaa aaggctgaca caattggatg
     1141 gggcaattaa gacggcgatg cagacgattc cggccaaaaa ccgaaagctc gtcacgtatc
     1201 atgattcatt tgcttatttc tgcccccggt acggatgtac cgtcatcgcg gcgattcagc
     1261 cgtctgactt ctcggaacct gcgccacgtg atatcgcggc cctgattgat cagctcaaga
     1321 cggagcagat tccggcgatc ttcggttcgg aggtgttccc gagtaaagtg atgaaccagg
     1381 tcggtaagga agccggtgta aaatttgtga ccaccttgcg ggacgatgac cttccgggtc
     1441 cggaaggctc ccctcagcat acctacattg ggatgataat cgaggatgtt cgtaccatcg
     1501 tccgatctct tggcggaaac gatgattcgc tgaaagagat caaaccggaa gaccggacaa
     1561 gctaggaaaa gttcatgcag cctctggtag aattagaaaa cgttacctgc ggttatgatc
     1621 accactcgat tttcgaggag gtgaatctcc ggattgcaca gggtcagctg gccgggattg
     1681 taggtccgac cggctccgga aagacgaccc ttctcaaggc tattctgggt atggtaaagc
     1741 cgatggccgg tacaattcgg gtgatgggcc ggtctgtttc tgggcagagc attgcccgaa
     1801 tcggttatgt cccccaactg gagaccgtgg actggaattt tcctgtcacg gtggagcagg
     1861 tggtggtgat gggacgttat cgggagatgg gttggatgcc ctggccgagc cgaaaagatc
     1921 gtcacgccgt gatagggctt ttggaacgtt tggggatggg ggcatttgtg aatcgtcaca
     1981 tccgagagct ttccggagga cagcagcagc gtgt
//
LOCUS       DATMKH010000111         1248 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1018161, whole genome shotgun sequence.
ACCESSION   DATMKH010000111 DATMKH010000000
VERSION     DATMKH010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1248
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1018161"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..>1248
                     /locus_tag="VNV63_01370"
     CDS             71..>1248
                     /locus_tag="VNV63_01370"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXC61298.1"
                     /translation="MTALYPQARQRFEAFREVSPNDRLVPSALLYIGRSYLEQGENEQ
                     AVSFYRQVWTRWPDLPQAQEAQLYLDQLQAKAVSVPAPVPEERLMRVRRLTDALRYDQ
                     ALKEAETLFVTEAGGPLRKPLLQQIGSLQYQLRRYESASRSFETLAQELIDQPDYTEI
                     VVWLGRIAYRKGDEIRLAQLEEKLAAQPAERDRPEHARLLSLLGNLYEDHKKTEEAEA
                     VYRRFVETFPQDPLAQEAAWRLGWMAYRTGRSDQAVAEFERFLLAYPQSPMAIQVLYW
                     KAKSLEKLGEIAQASSAYHQTCGITHHSFYCHRAEEQLRALPPIMGEGNSDDRDPALS
                     EREPSAVSEGPKGDLGVFASPVIQELYLMGLETEAGDELNRLVLRARPDRAPPGDTRA
                     P"
BASE COUNT          281 a          335 c          368 g          264 t
ORIGIN      
        1 cttgagggca cgaacaccgg agagcatctg gactccacag tcctggtatc gctccgggga
       61 ggccgccttt atgacggccc tttatcctca ggcccgccag cgcttcgaag ccttccgtga
      121 agtttctccc aatgaccgac ttgttccttc cgcgcttctc tacatcggac gatcctatct
      181 tgagcaaggt gaaaacgaac aggcggtgag tttctaccgt caggtctgga caagatggcc
      241 ggatctcccg caggcccagg aggcacagct ctatttggat caattacaag ccaaggccgt
      301 ttcggtcccc gctcccgtgc cggaagagcg cctaatgcgg gtgagacgcc tgacggatgc
      361 gttgcgttat gatcaggcat taaaagaggc agagacgctt tttgtgacgg aggccggcgg
      421 ccctcttcga aagccgctgc tacaacaaat aggcagtctt cagtatcagc tccgtcgcta
      481 cgaatccgca agccgatcgt tcgagacgtt ggctcaagag ctgattgacc agccggatta
      541 tactgagatc gtggtctggc tgggaagaat tgcctatcgc aagggagatg agatccgctt
      601 ggcgcaatta gaggaaaagc ttgcagcaca gcctgcggag cgggaccgcc ccgagcatgc
      661 ccggctctta tctcttcttg gaaatttgta tgaggatcac aaaaaaacag aggaggctga
      721 agcggtctat cgccgatttg tggaaacatt tccccaagac ccgctggccc aggaagcggc
      781 atggcgcctt ggttggatgg cttaccggac aggccgttcg gatcaggccg tcgcggagtt
      841 tgagcgattc cttttagcct acccgcagag cccaatggcg atccaggttc tgtactggaa
      901 agcaaagagc ctggagaaac tgggagagat tgcccaagca tcctccgcgt atcatcagac
      961 ttgtgggatc acgcaccaca gcttctactg tcatcgagcg gaagaacaat tgagagcgct
     1021 tccgcctatt atgggagagg gaaactcaga tgatcgcgat ccggcattat ctgaacggga
     1081 gccttccgct gtatcagaag gaccgaaagg tgatttgggt gtctttgctt ctccggtcat
     1141 tcaggagttg tacctgatgg gattagaaac ggaggcgggg gatgagctga accgtctcgt
     1201 tctgagggca agaccggacc gggcccctcc gggagatacg cgggcccc
//
LOCUS       DATMKH010000112         1618 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3903762, whole genome shotgun sequence.
ACCESSION   DATMKH010000112 DATMKH010000000
VERSION     DATMKH010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3903762"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1373
                     /locus_tag="VNV63_01375"
     CDS             <1..1373
                     /locus_tag="VNV63_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXC61299.1"
                     /translation="ERERRTRQLEIERQAVKKEKDAASAARLEKIEADLAKLREETAS
                     LKLKWQAEKGIIQKIRELKEQIEQTRISAEKAEREGDLGRASELRYGQLTELSKKLEE
                     ENRNMQELQVQGKMLKEEVDEEDIAEIVSKWTRIPVSRMLEGEIQKLIHIEERLKQRV
                     VGQDEAILAVSNAIRRARAGIQDPNHPLGSFIFMGPTGVGKTELARALAEFLFDDEQA
                     LIRLDMSEYMEKHAVSRLIGAPPGYVGFEEGGQLTEAVRRKPYSVILFDEIEKAHPEL
                     FSILLQVLEDGRLTDGKGRTVDFKNTVIIMTSNVGSQYIQDLIGKDEEEMRRQVMAAL
                     RAQFRPEFLNRIDDIILFRPLGLEELKAIVDIQLARLKTRLSEKKLTLLLTEQAKTFL
                     AQTGYDRLYGARPLKRIIQRDILNPLAVELLQGKFHEGDTIEIDEKKGRLVFQQLEAV
                     SKKTKK"
BASE COUNT          462 a          398 c          434 g          324 t
ORIGIN      
        1 atgaacggga acgacgaacc cgtcaactgg agattgaacg acaggcggtc aagaaagaga
       61 aggatgctgc ttcggctgcg cgtcttgaaa aaatcgaggc ggacctcgct aaactgcggg
      121 aagaaacggc gtcgctgaag cttaagtggc aggccgagaa aggaatcatc caaaaaatcc
      181 gggaattaaa agaacagatc gagcagacac gtatctcggc tgagaaagcg gagcgtgaag
      241 gagatctggg ccgcgcctca gaacttcgct acggacaact aaccgaactc tcaaaaaaac
      301 tggaggaaga aaaccggaac atgcaagaac tccaggtcca ggggaagatg ctgaaggaag
      361 aggtggatga agaagatatt gcagagatcg tttccaaatg gacgcgcatt ccggtcagca
      421 gaatgctgga gggtgagata caaaaactta tccacatcga ggagcgctta aagcaacgcg
      481 tggttggaca ggacgaggcg atcctcgcgg tctcgaacgc aattcgaagg gcccgcgccg
      541 gaatccagga tccaaatcac cctctgggtt cattcatttt tatgggaccg acaggcgttg
      601 gcaaaaccga gctggcccgg gccttggccg aatttctgtt tgatgatgaa caggcattga
      661 tccggctgga catgtccgaa tacatggaaa aacatgctgt ttcccgtctc attggggcac
      721 cgccgggtta tgtcggtttc gaagaagggg gacagttgac cgaggcggtc cggcgaaagc
      781 cctactccgt catcctgttc gatgagatcg agaaggccca cccggaactg ttcagcatcc
      841 tccttcaagt cttggaagac ggccgtttaa ccgatgggaa aggccgcacg gtggatttca
      901 agaacaccgt gatcatcatg acctcgaatg tcggaagtca atacatccag gacctgatcg
      961 gaaaggatga agaggagatg aggcgtcagg tgatggctgc gcttcgggcc cagttccggc
     1021 cggagttttt aaaccgcatt gatgatatca ttctgttccg cccgctgggc ctggaagaac
     1081 tcaaggccat cgtagacatt cagctggcgc gccttaagac acgcctctct gaaaagaagc
     1141 taacactcct gctcacggag caggccaaga ccttccttgc acagacagga tacgaccggc
     1201 tctacggcgc aaggcccctg aagcgaatca tacagcgtga tatcctaaac ccgctcgcgg
     1261 ttgagctcct gcagggaaaa ttccacgaag gagacaccat cgaaatagat gagaaaaaag
     1321 gccgattagt tttccaacaa ctcgaagctg tttcaaagaa aacaaaaaaa taatccatcg
     1381 gaatttccac gcatttttct tattgacaga tctctgttca atgtggtaga aaacatcacc
     1441 gttaagcggt gccatccgcg ctgaaatctt tcccttggag tcgttcatgc ccccaaagca
     1501 aatcctgctg tttaatgagg acccttctct tctcggcctt ctgaaggaag gttttgctca
     1561 aaacggcacc atcattactt catatcagga agcacaacaa aatgggaaga ggattcat
//
LOCUS       DATMKH010000113         1622 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1018752, whole genome shotgun sequence.
ACCESSION   DATMKH010000113 DATMKH010000000
VERSION     DATMKH010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1018752"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1622
                     /locus_tag="VNV63_01380"
     CDS             <1..>1622
                     /locus_tag="VNV63_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009381597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HXC61300.1"
                     /translation="ALVLSVIGIISPMIGLKTKNPAWVRTGRQAVTLIFVLLAIGMAS
                     LVYSFLIRDFSVKYVALTSNSKLPWFYTVTALWGGHEGSLLLWVFILSAYCTVAAWLH
                     WKTQPAVMPYLLIIQSIIMFGFLTLIIFLSSPFERYFPAPIDGKDLNPLLQDPAMVLH
                     PPMLYMGYVGFSIPFCFAMAALFSGRLGEEWIKVTQRWTLFAWMCLTTGILMGGYWAY
                     YELGWGGYWGWDPVENASFMPWLVGTAFLHSVMVQEKRKMFKVWNLFLIIVTFSLSLI
                     GTFLARSGILTSVHTFATDPQRGLYILTFLTMMMTLSFGTLILRSPKLKSQIELDSVI
                     SRESVFLFNNLFFLVAAVTVFLGTLYPLFIETVKGDKVTVGPPYYNAVFMPVALGLTF
                     LMGIGPTIAWRKASVENLKKNYVVPGIIALIGLLIFLVLGIRNSYALAGITVVCFVAT
                     TIFLDFGKISIFWAKRLRVDYLRGLLTAFTKNQRHYAGLVTHLGVLVLIIGIISSTIY
                     QKEKVVSMHIGDEFQLGPYIFKFADLHDVRGP"
BASE COUNT          303 a          465 c          395 g          459 t
ORIGIN      
        1 ggcgctggtt ctttccgtta tcggaatcat cagcccgatg atcgggctga agacaaaaaa
       61 ccctgcctgg gtccgtaccg gccgtcaggc ggtcacgctg atctttgtcc tgcttgccat
      121 cggcatggcc tcgttggtct attcgtttct gatccgtgat ttctccgtta aatacgtcgc
      181 gcttacctcg aacagcaagc tcccatggtt ttataccgtc acggccctct ggggaggtca
      241 tgaaggctcg ctgctcctct gggtcttcat actgtccgcc tactgcaccg tggccgcctg
      301 gctccactgg aaaacgcaac cggctgtgat gccgtatctc ctgatcatac agtctattat
      361 catgttcggg ttcctgacac tgatcatctt cctctcaagc ccgtttgagc gctatttccc
      421 ggcaccgatc gacggcaagg acttgaatcc tttgcttcaa gatccggcca tggtgctgca
      481 tccaccgatg ctctatatgg gctatgtcgg tttttcaatt cctttttgct ttgccatggc
      541 ggctctcttc tccggaaggc taggcgagga atggatcaaa gttacccagc ggtggacact
      601 cttcgcctgg atgtgtctga cgaccggtat tcttatgggt ggctactggg cttattatga
      661 actcggctgg ggtgggtact ggggttggga ccccgtggag aatgcctcat ttatgccctg
      721 gctggtgggg accgcttttc ttcattcggt tatggttcag gaaaaacgga agatgtttaa
      781 ggtctggaat ctgtttttaa ttatcgtgac cttctccctt tcgctcatcg gcacattcct
      841 ggcgcgtagc ggtatcctga cctccgttca tacgtttgcc accgaccccc aacgggggct
      901 ctacatcctc acgttcctca ccatgatgat gaccctatcc ttcggaactc ttatcttgcg
      961 ctcacccaaa ctgaagagcc agatcgagtt ggactccgtc atctcgaggg aatccgtttt
     1021 tctcttcaac aacctctttt ttctcgttgc cgcggtcacg gtctttctgg gaaccctcta
     1081 tcctctgttt atcgagaccg tcaagggcga caaggtcacg gtcgggcctc cttattataa
     1141 tgccgtcttt atgcccgtcg ctctcggcct gaccttcctc atgggaatcg ggcccactat
     1201 cgcctggcgc aaggcctccg tcgagaacct gaaaaaaaat tatgtggttc caggtatcat
     1261 tgccctaatc ggccttctaa tctttttagt cctaggtata cgcaacagct atgctcttgc
     1321 ggggataacg gttgtctgtt tcgtggcaac cacgatcttt ttagacttcg gcaagatctc
     1381 catcttttgg gcaaagcgcc ttcgggttga ttacctgcgc ggcctcttga ccgccttcac
     1441 caaaaatcaa agacactatg ccggactggt cacccacctg ggcgtcctcg tgctgatcat
     1501 cggcattatc tcctcaacga tctatcagaa ggaaaaggtc gtcagcatgc atatcggcga
     1561 tgagttccag cttggcccgt atatcttcaa gttcgctgat ctccacgatg tgcgcgggcc
     1621 ca
//
LOCUS       DATMKH010000114         4007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3263463, whole genome shotgun sequence.
ACCESSION   DATMKH010000114 DATMKH010000000
VERSION     DATMKH010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4007
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3263463"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="VNV63_01385"
     CDS             complement(<1..318)
                     /locus_tag="VNV63_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HXC61301.1"
                     /translation="MSFVESLRRVTNNLKDSKGIALVGMDGIVVEEHKKDPTLDLQTL
                     GAEYSAMMREVQRTNSALQIGDVKEVSVLTDKVIVISRQISKEYFLVLVVESEGNFGK
                     GRFL"
     gene            complement(315..1463)
                     /locus_tag="VNV63_01390"
     CDS             complement(315..1463)
                     /locus_tag="VNV63_01390"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXC61302.1"
                     /translation="MKNEDKQRGFLSPEILKLSERLAEDPTSKLFMPLGEEYFKAGML
                     EEAVMVLMDGLKAHPGFTSAHVTLGKVYLEKGQFKEAKEQFETAIQSSPDNLLAHRKL
                     VKIYSEEGAIQKAIQSCRVILSSDPKDEEIKKALAELGARQRDIATAQNLQPELVRTQ
                     HADLTRDTSSPVLEEKPEQAEEHNSIPTKEKGMEYAIELEEEKQEVIASPTSEEREKG
                     RVIELEDDEGSFEEIMNLMGQRGKIEPAAKATEEELATESLAELYMKQGFYNKGAEIY
                     QALVSKDPKNETLLKKLKDAVTLSKNASKTPVESNEAKAERPASMEIPNTTGPAVVTE
                     EAHAEARSKPEAPHSSTPFKPSPAQWSKAEKIQKLQAWLEQVKRSQKQ"
     gene            complement(1560..2750)
                     /gene="coaBC"
                     /locus_tag="VNV63_01395"
     CDS             complement(1560..2750)
                     /gene="coaBC"
                     /locus_tag="VNV63_01395"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HXC61303.1"
                     /translation="MQLANKKIVLGVTGSIAAYKAVLLLRELSERGADVAVVMTPSAQ
                     RFVTPLTFQVLSRQPVHTDLFEPRKEILHLTLAEEANLVLIAPATAHFIARAAHGMAD
                     DLLTALMLATTAPVILAPAMDGNMWDHPMVQQNIARLCEIGVRIVEPEVGPLASGNEG
                     KGRLAAEDRILEAVFDCFERKRDLAKELVLVTAGPTQEPIDPIRFLSNRSSGKMGYAM
                     AEAARDRGARVILISGPTALPKPAGIEFVEVRTSEEMYQAVKKFFPKVTMLIMAAAVA
                     DFRPQETGVQKRKKDGMPQTLGLEKTPDILAEVSRDKEGRLVVGFAAETHDLINQAKT
                     KLQKKNLDLIVANDVTMEGAGFEVDTNIVTLLDRSGLIIKLPKLSKREVAGRILDHVT
                     KMRR"
     gene            complement(2771..3139)
                     /gene="rpoZ"
                     /locus_tag="VNV63_01400"
     CDS             complement(2771..3139)
                     /gene="rpoZ"
                     /locus_tag="VNV63_01400"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HXC61304.1"
                     /translation="MTDITSLPITIKGDTIDTRYRLVIIASQRTKQLMQGRKPMIETK
                     AKKPTSIALDEVLSGKLEFFTGKEARQALKDARRLKEEEAKVKALMPRPSDIDELKKD
                     LSVYVNDSKERVDLVEEEEV"
     gene            complement(3136..3771)
                     /gene="gmk"
                     /locus_tag="VNV63_01405"
     CDS             complement(3136..3771)
                     /gene="gmk"
                     /locus_tag="VNV63_01405"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HXC61305.1"
                     /translation="MKRKGQLFVVSAPSGTGKTTICREVLKMVPDLKLSISYTTRPPR
                     PIEKDGRDYFFVAESRFRDMIRQEAFAEWAQVHGHSYGTPRKALLASLDSGHDVLLNI
                     DSQGAMQLKKYFPQAVFVYLLPPSFKTLKARLMERAADPSEVINKRLEKATEEIRNYS
                     AYSYLIVNDDLKKAIDDLRAIIQAERLKIHLVDHAWAARAFHLNKEGGKVE"
     gene            complement(3788..>4007)
                     /locus_tag="VNV63_01410"
     CDS             complement(3788..>4007)
                     /locus_tag="VNV63_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1732 domain-containing protein"
                     /protein_id="HXC61306.1"
                     /translation="EEITRLQSHAKQFEQMISGGQTVGRSLDFLIQEMNREVNTIGSK
                     GNDVVIAREVVYMKAELEKLREQVQNIE"
BASE COUNT          857 a         1111 c          908 g         1131 t
ORIGIN      
        1 caaaaaacgc cccttcccaa aattcccttc ggattccacc accaagacaa gaaaatattc
       61 tttgctgatc tgtcgagaga tcacaatcac cttatccgta agcaccgaga cctctttgac
      121 atcgccgatc tgcaaagcag agttggtcct ttgtacctca cgcatcatgg cactgtactc
      181 agcccccaga gtctggagat ctagcgttgg atccttcttg tgctcttcta caacaatgcc
      241 gtccatacca acaagcgcga tccctttcga atccttaaga ttgttcgtaa cacgtcgcag
      301 gctttccaca aaactcattg tttttggctc cttttgacct gttccagcca agcttgcaat
      361 ttttgaattt tctctgcttt ggaccactgt gcaggggatg gcttaaaggg tgtggatgaa
      421 tggggagcct ccggtttgga ccgcgcttcc gcgtgggctt cttcggtcac aactgctggt
      481 cctgtcgtat taggaatctc catcgaagca ggcctctcgg ctttcgcctc gtttgattct
      541 accggtgttt ttgatgcatt ttttgagagc gtgacggcat cttttaattt tttgagaagg
      601 gtttcatttt taggatcttt tgaaaccagg gcctgataga tctcagcccc tttattataa
      661 aatccttgct tcatgtacaa ctcggccaag gattctgttg ccagctcctc ttctgttgct
      721 tttgcggccg gttctatttt ccccctctgt cccatcaggt tcataatctc ttcgaatgat
      781 ccttcgtcgt cctcaagctc tatcacacgg cctttctctc tctcttcaga agtcggcgat
      841 gcgatcacct cttgcttttc ttcttcgagt tcgatcgcgt actccattcc cttctctttt
      901 gtgggtatcg aattgtgttc ttccgcctgt tccggctttt cctccagaac aggggaggag
      961 gtgtctctgg ttaaatcagc gtgttgggtt ctgactaatt ccggctgcag attttgagct
     1021 gttgcaatgt ccctctggcg ggcccctaac tccgccaggg ctttttttat ttcttcatcc
     1081 ttcggatccg aactcaatat cactcggcaa gactggatcg ctttctgaat ggccccctcc
     1141 tcgctataga tcttgaccag tttccggtgg gccaagagat tatctggact tgattgtatc
     1201 gcggtttcaa actgctcctt tgcctctttg aactgtcctt tttccagata aaccttgcca
     1261 agtgtcacgt gtgcgctggt aaaaccggga tgggctttga gaccatccat caaaaccatc
     1321 acggcttctt ccagcattcc ggccttgaag tactcctccc cgagaggcat gaagagctta
     1381 gaggtggggt cttcggcaag ccgctctgaa agcttgagga tctctggaga caagaaccct
     1441 ctctgcttat cctcgttctt catcggagac aggcactcct agttatatca ctctgaacag
     1501 caaattatat cataacaaat aaaccagaaa agtcaaggaa ttccagacga tgatgtgaat
     1561 catcgcctca ttttagtcac atgatccaga attcttccag ccacctcccg cttggatagt
     1621 tttgggagct tgattatcag accggacctg tccagcagtg tcacgatatt ggtgtccacc
     1681 tcaaacccag ctccctccat cgtgacgtca ttggctacaa tcaggtccag gttcttcttt
     1741 tgcagtttgg tctttgcttg gttgatgaga tcatgcgtct cggccgcgaa accaaccacc
     1801 aaacgaccct ctttatctct cgatacttcc gccaggatat ccggggtctt ttctaatcca
     1861 agagtctgcg gcatcccatc ttttttcctt ttctgcacac cagtctcctg cggcctaaaa
     1921 tcggccaccg ccgctgccat gatcagcatt gtgactttcg ggaaaaattt cttcaccgcc
     1981 tgatacatct cttccgaagt cctcacctct acaaactcga tcccagccgg tttcggaagg
     2041 gcggtcggtc cgctgatgag gatcacgcgt gctcccctgt ctcttgctgc ctccgccatg
     2101 gcataaccca ttttgccaga cgaccgattt gagagaaacc tgataggatc aatcggttct
     2161 tgggtcggac cggccgtcac cagcaccagc tccttggcca agtcccgctt tctttcgaaa
     2221 cagtcgaaga ccgcttccag tatccgatcc tcagccgcca gtctcccctt tccttcattg
     2281 cccgaggcca ggggtccgac ctctggttcc acaattcgga cgccgatctc acacaaccgc
     2341 gcgatgtttt gctgaaccat cggatgatcc cacatgttcc catccatcgc gggcgccaga
     2401 atgacaggag cggtagtggc aagcatcaag gcggtcagga gatcatccgc catcccatga
     2461 gctgctcgtg cgataaaatg ggctgtagcc ggggcgatca agacaaggtt ggcctcctcc
     2521 gccagggtca ggtgaaggat ttctttccta ggttcaaaga ggtctgtgtg gaccggttgc
     2581 cttgagagga cctggaatgt caacggggtc acaaaacgct gggcagaagg agtcataacc
     2641 acggctacat cggcgccgcg ctcgctcaac tctcgaagaa gcagaaccgc tttgtaggcg
     2701 gcgatactcc ccgtgacacc cagaacaatt tttttgttcg caagctgcat cacatagggt
     2761 tccggcctga tcagacttct tcttcctcta ccagatcaac ccgttccttg gagtcgttca
     2821 cataaacgct cagatccttc ttgagctcat ctatatcaga aggcctgggc atgagcgcct
     2881 tgaccttggc ttcttcctct ttcaagcgcc gtgcatcttt taaggcctgc ctggcttctt
     2941 tgccggtgaa aaattctagt ttacccgaca ggacttcatc taaagcaatg gaagtcggtt
     3001 tttttgcctt ggtctcgatc atcggttttc tcccttgcat cagctgcttg gtcctctgag
     3061 aagcgataat gaccaggcga tagcgggtat caatggtatc ccctttaatg gtgatgggga
     3121 gcgaagtaat gtctgtcatt ctacttttcc tccttcttta tttaaatgaa aagcacgggc
     3181 cgcccaggca tgatccacaa gatggatctt caagcgctct gcctggataa ttgctctgag
     3241 atcatcaatg gccttcttta agtcatcgtt cacaatgaga taagaataag ccgaataatt
     3301 tcggatctcc tctgttgcct tctcaagccg cttgttgata acttcggacg ggtctgcggc
     3361 acgctccatc aggcgcgcct ttaaggtctt aaaggaaggc ggcaggagat acacaaaaac
     3421 ggcctgagga aagtatttct taagctgcat ggcaccctgg gaatcaatgt tgaggaggac
     3481 atcgtgtccg ctgtcaagag aggcaagaag ggccttcctg ggagtcccgt aagagtggcc
     3541 atggacttgc gcccattccg caaaagcctc ttgtcggatc atgtcacgga acctggattc
     3601 ggcgacgaag aaatagtccc ttccatcctt ttctatcggt ctcggcggcc gagttgtata
     3661 ggagatggac aacttgagat cagggaccat ctttaaaacc tcacgacaga tcgtagtctt
     3721 tcctgtcccg gaaggtgcgg acaccacgaa taattgtcct tttcttttca tcctattttc
     3781 ttccgatcta ctcaatgttt tgaacctgct cacgaagttt ttccagttct gctttcatgt
     3841 aaacaacctc tcgggcaatg acgacatcgt tgcccttgga accgatcgta ttgacttctc
     3901 ggttcatctc ctggatcagg aaatccaggc tgcgccctac cgtttgacct ccggagatca
     3961 tctgctcaaa ctgtttggcg tgactctgaa gacgcgtgat ttcttcc
//
LOCUS       DATMKH010000115         1221 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3904292, whole genome shotgun sequence.
ACCESSION   DATMKH010000115 DATMKH010000000
VERSION     DATMKH010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3904292"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /locus_tag="VNV63_01415"
     CDS             complement(<1..773)
                     /locus_tag="VNV63_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008098863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HXC61307.1"
                     /translation="MQFAVQNSEPAVNIQAGARFSLGIDLGTTNSAVALEELETDRTG
                     MVEITQLLGPNRIGEKLTLPSAVYIPHPEEFPEEQLRLPWEQKSSGMIVGHFARDHGA
                     LVPDRLITSAKSWLSNFHIDPRKPVLPWKADISEPKVSPLECSRLYLEHMKLGFLNAE
                     RTQGRSWSIDEGQVVITVPASFDEVARNLTLEAARAAGFDKLILLEEPQAAFYAWMER
                     SGSDWRKQVSPGDTVLVCDVGGGTSDFSLIAIAEKEGNL"
     gene            complement(846..>1221)
                     /locus_tag="VNV63_01420"
     CDS             complement(846..>1221)
                     /locus_tag="VNV63_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002727340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2760 domain-containing protein"
                     /protein_id="HXC61308.1"
                     /translation="GEIAAFLALLQEKGRLVDFLMEDVTTYDDAQVGAAARVIHQGCK
                     EVLAEHFRITPVSVAKEGSPVTVPAGYAADEYRLIGKISGNPPFSGKLIHKGWKTDYV
                     KLPRIAKTSLLPTIAPAEVELQ"
BASE COUNT          271 a          341 c          324 g          285 t
ORIGIN      
        1 tcgaggttcc cctctttttc ggcaatcgcg atcagactga aatccgaggt gccgcctccg
       61 acgtcgcaga cgagaacagt gtcgccaggg ctgacctgct tgcgccaatc tgatccggac
      121 cgttccatcc aggcgtaaaa ggccgcctgc ggttcttcaa ggaggatcag cttatcgaac
      181 cctgcggctc gcgcagcctc gagcgtcaga ttccgcgcca cctcgtcaaa tgaggcgggg
      241 acagtgatga caacctggcc ttcatcaatg ctccacgatc ggccttgggt ccgttcagca
      301 tttaagaagc ccagcttcat atgctcgaga taaagccgcg aacattccag cggggaaacc
      361 tttggttcgc tgatatccgc tttccagggc agaaccggtt tcctcggatc gatgtggaag
      421 ttcgataacc aagacttggc tgaggtgatc aggcgatcag gaaccagggc accgtgatcc
      481 cttgcaaaat ggcccacgat catcccacta cttttctgct cccaaggaag gcgcagctgt
      541 tcttctggaa actcttctgg atgcggaata taaaccgcag acgggagggt tagcttttct
      601 ccaatccggt ttggaccaag gagttgtgtt atctcgacca tcccggttcg gtcggtctcc
      661 aactcctcga gagcaacggc actgttagtg gttcccagat caatcccgag gctgaagcgc
      721 gcgccggctt ggatgtttac tgcgggttcc gaattctgaa cggcaaattg cactgaagta
      781 aaattagagt gtcataacag aacctaaccg caagggaagt atcgcgctcg gaaacgagcc
      841 tctgcctact gaagttccac ctctgctgga gctatcgtag gaagaaggct ggttttagcg
      901 atcctaggta gcttgacata atccgtcttc caacccttgt gaatgagttt gccgctgaaa
      961 ggtgggtttc cgctaatctt cccgatgagc cggtactcgt cggcggcgta tcctgctggc
     1021 acggtgactg gagaaccttc tttagcgaca gaaacgggag tgatgcggaa gtgttctgct
     1081 agcacctctt tacaaccctg atgaattact cttgctgccg cgcccacctg tgcgtcatcg
     1141 taggtggtta cgtcctccat cagaaaatct accagccggc ccttttcctg aagaagagcg
     1201 aggaaagccg caatctctcc t
//
LOCUS       DATMKH010000116         1160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_697637,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000116 DATMKH010000000
VERSION     DATMKH010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_697637"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="VNV63_01425"
     CDS             complement(<1..415)
                     /locus_tag="VNV63_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018289934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HXC61309.1"
                     /translation="MKGYQKPEDLTGPGGIMEQLTKRLYERVLGAEMTHYLGYEKGQA
                     PKLGADEKRENHRNGTSKKTLVSEDGKLEIEVPRDRAGEFEPQFIRKGQRRFGGFDTK
                     IIAMYAQGMTVREIKAFLEEQYKVEISADLISTVTN"
     gene            complement(639..>1160)
                     /locus_tag="VNV63_01430"
     CDS             complement(639..>1160)
                     /locus_tag="VNV63_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HXC61310.1"
                     /translation="GLSYMGAKQHCAELEKQGVVDTWRRPKPVGRPELVYRLTPKAGV
                     FFPAETNLSTIEILHAARHLFGATASDKLLLALFRKKAESYARNLRGDTREARAEALS
                     RLRDAEGHLSEVVKAPDLMIQEYHSPILDLLDAFPLVRRLEKEMFETLLGASVERHEE
                     RASGLYLCTFLIS"
BASE COUNT          237 a          355 c          270 g          298 t
ORIGIN      
        1 aattggtcac tgtactgatc agatcagccg agatctccac cttgtattgt tcttccaaga
       61 aggctttgat ctcgcgcacc gtcatgcctt gggcgtacat ggcgatgatt ttggtatcaa
      121 agcctccaaa gcggcgttgc ccttttcgaa taaactgagg ctcaaactcc ccggctcgat
      181 cgcgtggcac ttcgatctct agcttcccat cctcgcttac cagcgtcttt ttgctcgtac
      241 cgttacgatg gttctctcgc ttctcatcgg ctcctagctt cggcgcctgg cctttttcgt
      301 acccaagata atgggtcatc tcggccccaa gtacccgttc gtacagccgc ttagtcagct
      361 gttccattat ccccccagga ccggtcaaat cttccggctt ctggtaaccc ttcatcagct
      421 cttcgatgat cgccggatct atccccggct ttttgccttc ctggcactgc gcttcttgct
      481 ccttcttctt ggtcacttcc atctgtcttt acctcaggtt gtgacccaca cacaaattta
      541 ctgacaccct ctactccgac gctccctcac tccaatactc catcacttcg ctcgccagaa
      601 ttcgaggacg aggacgagaa cgaagcgcct cacgcgcttt aactgatcag aaacgtacaa
      661 aggtagaggc cggaagcccg ttcctcatgc cgctcgacgg atgctccaag gagagtctca
      721 aacatctctt tctccaagcg cctaaccagc ggaaaagcat cgagcagatc caggattggg
      781 gaatgatatt cttggatcat gaggtccggc gcttttacaa cctcggaaag atggccctcg
      841 gcatcgcgta ggcgggacaa agcttcggcc cgagcctcac gggtgtcccc acgtagattt
      901 cgggcgtagc tttccgcctt ctttctgaag agagccagca atagcttgtc ggaggcggta
      961 gcgccgaata gatgacgcgc cgcatggaga atttcgatcg tgctcaggtt ggtctcagcc
     1021 ggaaaaaata cacctgcttt gggtgttaac cgatagacca attccgggcg tccaactggt
     1081 tttgggcgcc gccaggtatc gactacaccc tgcttttcca attcggcgca atgctgtttg
     1141 gcgcccatat aactcagacc
//
LOCUS       DATMKH010000117         1184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2293914, whole genome shotgun sequence.
ACCESSION   DATMKH010000117 DATMKH010000000
VERSION     DATMKH010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1184
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2293914"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VNV63_01435"
     CDS             <1..205
                     /locus_tag="VNV63_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61311.1"
                     /translation="KRNLTITHCLNRIKYGSVAPCHPSSFLKDLDTRFTEVINFHEVT
                     NKPAGEGTARLHFAKMRELLGGG"
     gene            complement(214..1131)
                     /locus_tag="VNV63_01440"
     CDS             complement(214..1131)
                     /locus_tag="VNV63_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HXC61312.1"
                     /translation="MSVLVVGSIAIDSVKTQLEERLEVLGGAASYASISASFFSPVNL
                     VGIVGTDFPKEYIELFKSRGINLDGMEVADGRTFRWSGEYDWDMNQRKTLDLELNVFE
                     TFQPKLPAAYKRTPIVLLGNISPVLQNHVLDQVEHPRFVVADTMDLWISTTKPPLLDL
                     IKRVDLLVLNDSEARQLTAETSLIKAGRALIKLGPKFVAIKKGEHGCLLLSHDHFFSC
                     GAFPLEDIQDPTGAGDTFAGGFTGYLAGLEKESFEFADLKKAVVYGSLLASFCVESFS
                     LDRLKTLSREDIEDRYHLFRVMSHFEVIE"
BASE COUNT          312 a          327 c          277 g          268 t
ORIGIN      
        1 caaacgcaac ctgaccatca cgcattgcct gaaccgcatc aaatacggca gcgtggcccc
       61 ttgccatccg agttcgtttt tgaaagatct ggatacacgc ttcacggaag tgattaactt
      121 ccatgaagtg acgaataagc cagccggtga aggaaccgcc cggctgcatt tcgccaagat
      181 gcgggagttg cttggaggag ggtgaaggct ggattattcg atcacttcga aatggctcat
      241 cactctgaac aagtgatacc tatcctcgat atcctccctg gaaagcgttt tcagacgatc
      301 caggctgaat gattcaacgc aaaagctagc cagcaaactt ccgtaaacca ccgccttctt
      361 caggtcggca aattcaaacg actccttttc caagcccgcc agataaccgg taaagccgcc
      421 ggcaaaagtg tcaccggcac cggtcgggtc ctggatatcc tccaagggaa acgctccgca
      481 actgaagaaa tggtcgtgac ttaggagcaa gcatccgtgt tcaccctttt taatcgccac
      541 aaatttgggg ccgagcttga tcagcgcacg accagccttg atcaaactag tctctgctgt
      601 taattgccgg gcctcactgt cgttcaacac cagaagatcc acgcgtttga tcagatcaag
      661 gagcggaggc ttggtcgtgg agatccaaag atccatggta tccgcaacta cgaaacgagg
      721 atgttctacc tgatcgagaa catgattttg caaaactgga ctgatgtttc ccaaaagaac
      781 gatcggggtc ctcttgtaag ccgccggcag tttcggttgg aaagtctcaa atacgttgag
      841 ctccagatca agcgtcttgc gctgattcat gtcccaatcg tattcgcccg accaacgaaa
      901 ggttcttcca tcggccacct ccatgccgtc caggttgatg cctcgcgact tgaagagttc
      961 gatatattcc tttggaaaat ccgtaccgac gatccccacc aagttgaccg gcgaaaagaa
     1021 acttgcactg attgatgcgt aagaagcggc tccgcccagg acttccagtc gctcctcgag
     1081 ctgcgtttta acactatcaa tagcaatgga tccaacgacg agaactgaca taggctataa
     1141 tcaaacaggc acctccgttt aacacattcc tgcttgatcg cacg
//
LOCUS       DATMKH010000118         1643 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1339570, whole genome shotgun sequence.
ACCESSION   DATMKH010000118 DATMKH010000000
VERSION     DATMKH010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1339570"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VNV63_01445"
     CDS             complement(<1..233)
                     /locus_tag="VNV63_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61313.1"
                     /translation="MKPGKDGSKGSYSGGDSSLMAEDAGPSVIGMSGINWRFADPQAD
                     SLASASFNNLSPSPLARFVMSALAANQGMKEAS"
     gene            complement(1019..1447)
                     /locus_tag="VNV63_01450"
     CDS             complement(1019..1447)
                     /locus_tag="VNV63_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61314.1"
                     /translation="MSLVEILRECRDVQLELAALIDGHAKEVESGVGVEVVNGYIQVL
                     NDIEPKLNRLFKDFFSKARTALYHLLGQGRASDSVTCFLLGRSVSFVQAKDDSKFEEG
                     AARFLRDIPRREGASSHEYPQGRPSGVELDIDRYSEPNNS"
BASE COUNT          392 a          477 c          398 g          376 t
ORIGIN      
        1 atgctggctt ccttcattcc ctggtttgcc gcgagcgcgg acatcacgaa acgggccaga
       61 ggcgacggtg acaggttgtt gaaactcgcg ctggcgagcg agtccgcctg cggatcggcg
      121 aatcgccagt tgatgccgct cataccgatc accgatggcc ccgcgtcctc cgccattaga
      181 gatgaatccc cacctgaata ggatcccttc gagccgtctt tccctggttt caccggcttg
      241 accgcgaagt ctcgcccctg acaataagga agtcccgggt tattaaaaca agcattctgc
      301 cagctaaatt ttctttgtcc gaaaagactg gtcacaagtg ccagagcaag cgcaaatgta
      361 cggaatttca catcttgatt ttgcgccgtt tgcgaggact ttaaaatagg gcgtaaccct
      421 gatatcgcat aaggcaaaaa cctctctgaa atgaacgtca cccaatcatt cttgtctgct
      481 cgctaagagt ggggcaggtt cccgacctgc ccttcgaccg acatcctccc aagagcgtcg
      541 acgtcttcct caccgaaccg cgaccgtcag ggagcggata gacgcttaaa tcctgcccga
      601 aagaatcatc tcagaggaat gccggacgga cgagaccgtc cctagccccg tttctccttt
      661 aggcaaaaac caggaaagcc aaaggtatta agaattcgcg gaaagtctct tccgtctccg
      721 gttcttcctt tcgggtcaca accgctacgg actgtcgctg cgaccaggac ttaaggcaaa
      781 gcaccatcga aatggcagtt tcgggtcaac ctgctcatcc gggatccgac acggcacaat
      841 attctcaggc atgcggatag cgatgcataa gagaaccgtc tcttccacgg cctgataaag
      901 attttcccag aatagattga gatactggcg taactcccgt cgactaacgg ttggaaaaaa
      961 tgcgcatact tttccttctg cgatacggta ggtcatcttc agctgtggac agtcgatatc
     1021 atgaattatt cggttccgag taccgatcaa tgtcgagctc cacgccgctc ggtctcccct
     1081 gaggatattc atgagagctt gcgccttctc gccggggaat gtcgcgcaga aacctcgccg
     1141 caccctcctc aaatttggaa tcatctttag cctgcacgaa actgacgctt cgcccgagaa
     1201 ggaaacacgt gactgaatcc gaggctctcc cctgcccgag gagatgatag agagctgttc
     1261 gggctttgct gaaaaaatcc ttaaagagcc ggttcaattt gggctcgatg tcattcagga
     1321 cctgtatgta cccgttcacg acttcgacac ccacaccgga ttccacctct ttggcatgac
     1381 catcaatgag cgcagccagt tctagttgga catcccggca ctccctgagt atctcgacaa
     1441 gcgacatctg gagtcgcttg aatcgctccg aatccagggt ctcgaaaggc ggcttcgctc
     1501 aaattgaata gctgaatcgt gagccggcaa agccattctg agtcctcggc ggtggtgaga
     1561 cgcgtgaaca tcttttagcc tctgtgccaa ttctacaacc ctcttcgcag ccccacctgg
     1621 acgtctaaag cttatctccg aaa
//
LOCUS       DATMKH010000119         1124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1659388, whole genome shotgun sequence.
ACCESSION   DATMKH010000119 DATMKH010000000
VERSION     DATMKH010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1659388"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="VNV63_01455"
     CDS             complement(<1..380)
                     /locus_tag="VNV63_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HXC61315.1"
                     /translation="MVILKSKDEIEKIRKACRVVATVLSELEAQVKPGRTTMELDQVA
                     EKMILKLGARTAFKGYRGFPATLCASINNEVVHGIPSQRALKEGDIIGLDLGAIVDGY
                     YGDAAITVSVGSIPDETERLIRVT"
     gene            complement(383..1057)
                     /locus_tag="VNV63_01460"
     CDS             complement(383..1057)
                     /locus_tag="VNV63_01460"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008287617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HXC61316.1"
                     /translation="MKRLVFLGPPGAGKGTQANKLSERYGLLQISTGDILREAVQHAT
                     PLGIQAQKYILSGALVPDEIVIGLIGERLKKISGQSNAGYILDGFPRTVPQAEALDKM
                     LRNERACLDKVLSFSLDETELISRLSGRRSCPSCHRIYHMEFNPPEKAGMCDRCQVAL
                     VMREDDKPETVRERFKVYHRQTAPLLDYYRKKGLLIEVNAGGSMEEVMKRVLQTIGDL
                     PVVSKV"
BASE COUNT          255 a          347 c          240 g          282 t
ORIGIN      
        1 tccgttaccc ggatcagtcg ttctgtctca tccggaatgg aacccaccga gaccgtaatc
       61 gccgcatcac cgtaatagcc gtccacaatc gctccaagat ccaagccgat gatgtctcct
      121 tccttcaagg cacgctggga agggatacca tgaaccacct cattattaat agaggcgcag
      181 agcgttgccg gaaaaccccg atagccttta aacgcagtcc tggctccgag tttgaggatc
      241 atcttttccg ccacctggtc caattccatc gtcgtcctcc ccggcttgac ttgggcctcg
      301 agttcagaaa gcactgtagc gacaacgcgg caagcttttc gaatcttctc aatctcatct
      361 ttcgatttta aaataaccat ctctaaacct tgctaacgac aggcagatct cctatcgtct
      421 ggaggacacg tttcatgacc tcctccatgc tgccccccgc attcacctcg atgagcaaac
      481 cttttttccg atagtagtcc aataatggcg ccgtctggcg atgataaacc ttaaaccgtt
      541 cacgaaccgt ttccggtttg tcatcctccc tcatgaccag ggcaacctga cagcggtcac
      601 acattccagc cttctcgggc ggattaaact ccatatgata aatcctatga cagcttgggc
      661 agcttcttct tcccgagagc cggctaatga gttcggtctc atccaaagaa aaactcagga
      721 ccttgtccag gcacgcacgt tcattccgca gcattttatc cagggcttcg gcctggggaa
      781 cggttctcgg aaatccatcc aggatgtacc ccgcatttga ttgaccgctg attttcttta
      841 agcgttcccc gataagtccg atcacgatct cgtccggtac aagtgctccg ctcaaaatat
      901 acttctgggc ctgaatgccc aggggtgttg cgtgctgcac cgcctcccgg agaatatctc
      961 cggttgagat ctggagcagt ccgtatcgct ccgataattt attggcctgc gtcccttttc
     1021 cggcacccgg ggggcccagg aaaaccaagc gcttcatctt acaccgccca tcaagagctg
     1081 cgccctctga tcttaccctt cttcataaaa ccttcataat gacg
//
LOCUS       DATMKH010000120         3866 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1659501, whole genome shotgun sequence.
ACCESSION   DATMKH010000120 DATMKH010000000
VERSION     DATMKH010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3866
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1659501"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..844
                     /locus_tag="VNV63_01465"
     CDS             <1..844
                     /locus_tag="VNV63_01465"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HXC61317.1"
                     /translation="VVLCSAIVLGLSAIVWAALRLVNMKEVHLFDGLFHVETVRVEGV
                     STDRAADVRAVIEKQPSVSLRELELEKIREALVQLRWVKEVLLRKEWPDALVVKITER
                     APLVWVEEQMPSEHRDRMPAKQGAGRYRLVDEDGVELESLDHPTAGFPVIRLNSISNI
                     PDSGQAGLQAGIHVLKVLAASGMEAAVLADIEMEVRDAQEIRLRYQGLPLRLGSGDTE
                     KQIRRFLLLKPELLAKASQISEVDLRFPGRLIVKSKAGFVGAYRRVAQIKGEAAPRPY
                     TEGR"
     gene            852..2084
                     /gene="ftsA"
                     /locus_tag="VNV63_01470"
     CDS             852..2084
                     /gene="ftsA"
                     /locus_tag="VNV63_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HXC61318.1"
                     /translation="MAKRENIVVGLDIGTTKICAVVGEINQNDQVDVIGIGTSPSRGL
                     RKGVVVNIESTVESIKRAVEEAELMAGVQINSVYAGIAGGHIKGFNSRGVIAVKDREV
                     NQSDVDRVIDAAKAVAIPLDREVLHVLPQEFIVDDQDGIRDPLGMSGVRLEAEVHIIT
                     GAVTSAQNIIKSVNRAGLEVADIILQPLAASCAVLTNDERELGVAMVDIGGGTTDIAT
                     FVEGSIWHTAVLGIGGNHLTNDIAIGLRTPTNEAEKIKIRYGCALSNLVREDETIEVP
                     SVGGRPTRVLSRQLLAEIIEPRAEEIFSLVSREIKRTGYEEMVASGVVITGGSSILEG
                     MPEVAEKVLDLPVRRGVPSHVGGLVDIVNNPMYSTAVGLILYALKEQGDQNLRRFTER
                     NLLNKVAHRMKSWVKEFF"
     gene            2103..3227
                     /gene="ftsZ"
                     /locus_tag="VNV63_01475"
     CDS             2103..3227
                     /gene="ftsZ"
                     /locus_tag="VNV63_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HXC61319.1"
                     /translation="MLLFDEEEYQVAKIKVIGVGGSGCNAVNTMIAARLSGVEFVSAN
                     TDLQALGTSKAPLKIQLGARLTKGLGAGSNPEMGREAALEDQDKIRDAITGADMVFIT
                     AGMGGGTGTGAAPIIAEVARDLGVLAVAVVTKPFTFEGFKRMQRAEEGLKELKKHVDT
                     LIVIPNQRLLNVVDKATPLIEAFKVTDDVLRQAVQGISDLITVPGLVNVDFADVKTVM
                     SHMGRAVMGMGISRGANRAVDAAQKAISSPLLEDGSIHGARGVLLNISGGPSLSLHEV
                     TEASSIIQSAADPEANIIFGSVISDKLANDVIVTVIATGFETEQERGEIKQPASHKAG
                     HSQVLDRPTFLRKVPPREFQREGITDDEWDVPTFLRKQLD"
     gene            3320..>3866
                     /locus_tag="VNV63_01480"
     CDS             3320..>3866
                     /locus_tag="VNV63_01480"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HXC61320.1"
                     /translation="MIQAMPETFKQKSSLGVVLTRLSANHGLRHAFGTKELANEERLA
                     QAMDQEGIPVVSVHQVHGDGILVLDQPAEDWAAAQKKTAAAGRVEGYDAYDAIVTDQT
                     GVILAVRTADCLPVLIFDPMQRVIAAVHAGWRGALREIVPKTVRCMMDRFSCRPAAII
                     VGVGPSVRSCCYEVGEMVLAPL"
BASE COUNT          874 a          854 c         1278 g          860 t
ORIGIN      
        1 ggtggttctt tgctccgcaa ttgttttagg attgtctgcg attgtttggg ccgccctgag
       61 gctggtgaac atgaaagaag ttcatctttt tgacggtcta tttcatgtcg agacagtccg
      121 agtcgaaggt gtctcaacgg atcgtgcggc cgatgtccgg gccgtgattg aaaaacaacc
      181 gagtgtgtcc ttgcgtgaac tggaactgga gaagatccgt gaggcgctgg tgcaactgcg
      241 gtgggtcaaa gaggtcctgc tccgaaagga atggccggat gctcttgtcg tcaagatcac
      301 ggagcgtgca ccgcttgttt gggtagaaga acagatgcct tccgaacata gagatcgcat
      361 gccggccaag cagggcgcgg gacgttatcg gttggtggat gaggacggcg ttgaactgga
      421 atctctggat catcccacgg caggctttcc ggttatccgt ttaaattcga tatcaaatat
      481 tcctgattcc ggccaagcag gactccaggc agggatccat gtcttgaagg ttttagccgc
      541 cagcggcatg gaggcggcgg ttttagcgga tatcgagatg gaggttcggg acgcgcagga
      601 gattcgactt cgataccagg gtctgccgct ccggctgggg tctggcgata cggagaaaca
      661 gatccggcgt tttctgcttc ttaagccgga gttgctggcc aaggcctctc agatctccga
      721 ggtggatctt cggtttccag ggcgattgat tgtaaaatcg aaggcgggtt ttgtaggtgc
      781 ataccggcgg gtcgcccaga taaagggcga ggcagcgcct cgcccctaca cggaagggag
      841 gtaaggccgc attggcaaaa agggaaaaca ttgttgtcgg attagatatc ggaaccacca
      901 agatctgtgc ggtggtgggc gagatcaacc agaatgatca agtggatgtg atcgggatcg
      961 gaacgagccc gtcgcgtggt ttgcgaaaag gcgtggtcgt gaatattgaa agcacggtag
     1021 agtccatcaa gcgggcggtt gaagaggccg agttgatggc cggagttcag attaattccg
     1081 tttatgcagg gatcgcaggg ggacatatca agggtttcaa cagtcgaggc gtgattgcgg
     1141 tcaaggaccg ggaggtgaat cagtcggatg tggatcgcgt cattgatgcg gccaaggcgg
     1201 tagcgatccc gctggatcgg gaggttcttc atgtccttcc acaggagttt atcgtggatg
     1261 accaggacgg gattagagat cccttgggga tgtcgggtgt ccggttggaa gcggaggtcc
     1321 atattattac cggggcggtg acctcggccc agaacattat caagagcgtg aaccgagccg
     1381 ggttggaggt ggcggatatc atattgcagc cattggccgc aagctgtgca gtcttgacaa
     1441 acgatgaacg ggaactgggc gtggcgatgg tagacatcgg gggagggacg actgatatcg
     1501 ctacctttgt cgaggggagc atctggcata cggcggtcct ggggatcggc ggaaaccacc
     1561 tcacaaatga tatcgcgatc gggttacgga caccgaccaa cgaagcggag aagatcaaga
     1621 tccggtacgg atgtgccttg agtaacctcg tgagagagga tgaaacgatt gaggtgccga
     1681 gcgtcggtgg gcgtcccacc cgcgtgctgt ccaggcagct tttagccgag atcattgaac
     1741 cgagagcgga agagatcttc agtctcgtga gccgggaaat caagcgcacc ggttatgaag
     1801 agatggtggc ctccggcgtg gtgatcacgg gagggagctc tattctggag ggtatgccgg
     1861 aggtggcgga aaaggtcttg gacctgccgg ttcggcgggg agttccgtct cacgtgggtg
     1921 gtttggtaga catcgtgaac aatccaatgt attccacggc agtggggttg atcttgtacg
     1981 cattgaagga gcagggcgat caaaatctca ggcgttttac ggagagaaat cttttaaaca
     2041 aggtggctca tcgaatgaag agctgggtca aggaattctt ttaacgaaag ggggaggacg
     2101 ccatgttact ctttgatgaa gaggaatatc aggttgcaaa gatcaaggtg atcggtgtgg
     2161 ggggaagcgg ctgtaacgcc gtgaacacca tgatcgcagc gcgattgtcc ggggtggagt
     2221 tcgtttccgc caatacggac ctccaagctt tggggacctc aaaggctcca ctgaaaattc
     2281 agctcggtgc caggttgacc aaagggctgg gggcgggatc gaatccggag atgggacgtg
     2341 aggcggctct ggaggatcag gacaagatac gggatgccat aaccggagcg gacatggtct
     2401 ttatcacggc tggaatgggc ggaggaaccg gtacaggcgc ggcccccatt attgccgaag
     2461 tggcccgcga tcttggggtc ttggccgtgg cggtcgtgac gaagccgttt acctttgagg
     2521 gttttaaacg catgcaacgg gctgaggagg gtttgaagga gctcaagaag catgtggata
     2581 cgctcatcgt gattccgaat caaaggctgc tcaatgttgt agacaaggcc acaccgctca
     2641 ttgaggcgtt taaggtgacg gatgatgtcc ttcgacaagc ggtgcagggg atctccgacc
     2701 tgattacggt gccggggttg gtcaacgtag attttgcgga tgtcaagacc gtgatgtccc
     2761 acatgggccg tgccgtcatg gggatgggga tctctcgtgg agccaaccgg gcggtggatg
     2821 cagcgcagaa ggcgatctca agccccttgc tggaggacgg ctcgattcat ggggcccgtg
     2881 gtgtcctgct caatatctcg ggcggtccaa gcctatcgct ccacgaggtg accgaggcct
     2941 cctccattat acaaagcgcg gccgatccgg aagcgaatat catctttggc tcggtgatca
     3001 gcgataaact ggcaaatgat gtgatcgtta cagtcatcgc gaccgggttt gaaacggagc
     3061 aagaaagagg ggagataaaa caaccggcca gccacaaggc aggccattcg caggttctgg
     3121 atcgtccaac ttttttaagg aaggtccccc cccgtgagtt tcagcgcgaa gggattacgg
     3181 atgatgaatg ggatgtcccg acatttttgc gtaagcagct ggattaacag gttttaaaag
     3241 tatcctgtta gaaatatctg gagattctga ggctaagagg atagaatagg gaactattag
     3301 gtttggatag ttttactgga tgattcaagc gatgccggag acttttaaac aaaaatcatc
     3361 ccttggggtg gtgctgaccc gtttatccgc aaatcatggt cttcgccacg cctttggcac
     3421 caaagagttg gcgaacgagg agcgtctggc ccaagcgatg gaccaggaag ggattccggt
     3481 ggtttcggtt catcaggtcc atggagatgg aattctcgtg ctggatcagc ctgcggaaga
     3541 ctgggccgcg gctcagaaaa aaacggcagc ggctggaagg gttgaaggtt atgatgccta
     3601 tgatgcgatc gttacggatc agactggagt catactggcg gtccgaaccg cagactgcct
     3661 tccggtctta atctttgatc caatgcagcg ggtcatcgct gcggttcatg ccgggtggcg
     3721 gggtgcgctt cgagaaatcg tgccaaagac ggtgcgatgc atgatggatc gtttttcatg
     3781 ccggcctgcg gcaatcatcg taggagtcgg gccttcggtt cggtcttgct gctacgaggt
     3841 gggggaaatg gtcctagcgc cgcttt
//
LOCUS       DATMKH010000121         1087 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1659851, whole genome shotgun sequence.
ACCESSION   DATMKH010000121 DATMKH010000000
VERSION     DATMKH010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1659851"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VNV63_01485"
     CDS             complement(<1..189)
                     /locus_tag="VNV63_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61321.1"
                     /translation="MANEMTQTTELKREAEVKHHTINIFVEVNGKDKKVEFDHSPVTG
                     AEIRSGAGVPASDDLTRLE"
     gene            410..973
                     /locus_tag="VNV63_01490"
     CDS             410..973
                     /locus_tag="VNV63_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61322.1"
                     /translation="MAAIESLSAEQFYRLKKFARFRVRGLGRAAIGETYISLLDEAVA
                     STLQGAEGGTRGRRWAKDRVPFMGHLFGAMRSISTHWKEAYERRGTESELLECETTSE
                     DEEGNLSWPGKRAIDPAADQFRTYAAKELLDALDKHFAEDQDALLVIEARKEGMTVSE
                     MVAGLGLTEKKANAALQRIRYFMERFL"
BASE COUNT          250 a          260 c          322 g          255 t
ORIGIN      
        1 ttcaaggcgt gtcaggtcat ccgatgcggg gacaccggct ccggaccgga tttccgctcc
       61 agtcacgggt gagtggtcaa actctacctt tttatccttg ccgttcactt caacgaagat
      121 gttgatcgtg tgatgtttaa cttcggcttc tctttttaat tcagtggttt gggtcatctc
      181 gttcgccatc aaaggcctcc tttatcggtg gattcacctt ccgttatcta tatgcccggg
      241 atttgggcta gtacgagact ctcgtgatcg cggttggtgc gccttgcttt tttgaagggt
      301 gcctctcacg tcgaccgaaa aaccgggcat agtataagtt ggaaccagaa tgcgcggaga
      361 tcgaggacgg agcacgcgaa ctgaggaatg tgcaacaacg gctgaggtga tggccgccat
      421 tgaatccttg tctgcagaac aattttacag gctgaaaaag ttcgcccgat tccgcgtgcg
      481 tggactaggc cgtgccgcga taggtgaaac ctatatttcc cttcttgatg aagccgtagc
      541 gtccacccta cagggggcgg aaggcggtac cagagggcgt aggtgggcga aggaccgcgt
      601 cccatttatg ggccatcttt tcggggcgat gcgaagcatc tcaactcatt ggaaggaagc
      661 ctatgagcgc cgaggaacgg aatcagaact gttggaatgc gagaccacaa gcgaagatga
      721 agaagggaat ctctcgtggc ccgggaagcg ggcgattgat ccggccgcag atcagtttcg
      781 cacatatgcc gcaaaagaat tgctcgatgc acttgacaag cactttgccg aagaccagga
      841 tgcgttgctt gtgattgagg cccgcaagga aggtatgacc gtctcggaga tggttgccgg
      901 attgggtctc acggaaaaaa aggccaatgc tgcattgcag cgtatccggt attttatgga
      961 aaggtttttg tagtgccaaa gtttgacaaa cacgaagagg atctgatgcg tctgatgttc
     1021 ggccttgccg agtcgctcgc cgaagcgcca gagccggaga ttctcgaaga agccgggcag
     1081 aaccacg
//
LOCUS       DATMKH010000122         1631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1340742, whole genome shotgun sequence.
ACCESSION   DATMKH010000122 DATMKH010000000
VERSION     DATMKH010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1340742"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="VNV63_01495"
     CDS             <1..334
                     /locus_tag="VNV63_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014395882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HXC61323.1"
                     /translation="RLSDISHAIQEHVEKAGFSVVTDFVGHGIGRNLHEDPQIPNFGK
                     PGQGLRLRSGMTLAIEPMVNMGSGAVKILEDNWTAVTQDGRLSAHFEHTVLVGERGAE
                     ILTERPSS"
     gene            351..569
                     /gene="infA"
                     /locus_tag="VNV63_01500"
     CDS             351..569
                     /gene="infA"
                     /locus_tag="VNV63_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HXC61324.1"
                     /translation="MAKEDVVEVQGTIVETLPNAMFRVELENGHRVLAHISGKMRMHF
                     IKILPGDKVTVELSPYDLTRGRITYRFK"
     gene            582..695
                     /gene="rpmJ"
                     /locus_tag="VNV63_01505"
     CDS             582..695
                     /gene="rpmJ"
                     /locus_tag="VNV63_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015851088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HXC61325.1"
                     /translation="MKVRASVKAICSKCKVIRRKGVVRVICENQRHKQRQG"
     gene            731..1114
                     /gene="rpsM"
                     /locus_tag="VNV63_01510"
     CDS             731..1114
                     /gene="rpsM"
                     /locus_tag="VNV63_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HXC61326.1"
                     /translation="MARIAGIDLPREKRIEVALCYIYGIGRSASQKILKELKISPDVR
                     VKDLKEEDSVRLREKIEREYKVEGDLRKEVAMNIKRLMDIGSFRGLRHRKHLPVRGQR
                     TKTNARTRKGPTKAIGVRPKKEQTA"
     gene            1131..1508
                     /gene="rpsK"
                     /locus_tag="VNV63_01515"
     CDS             1131..1508
                     /gene="rpsK"
                     /locus_tag="VNV63_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014015761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HXC61327.1"
                     /translation="MKKGKKKERKIVQTGVAYIQASFNNTIVTVTDMSGNVVVWASAG
                     GQGFKGSRKSTPFAAQRAAETAAKKAMEHGMRQVDVHVKGPGSGRESAIRALQAAGLK
                     INLIKDVTPIPHNGCRAPKRRRV"
     gene            1523..>1631
                     /locus_tag="VNV63_01520"
     CDS             1523..>1631
                     /locus_tag="VNV63_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HXC61328.1"
                     /translation="MARYIGSVCRLCRREGIKLYLKGQRCFTDKCAIERR"
BASE COUNT          446 a          344 c          495 g          346 t
ORIGIN      
        1 aagactttct gatatctcgc atgcgattca ggagcatgtg gagaaggccg gtttctcggt
       61 cgtgaccgat tttgtcgggc acgggattgg ccgcaatctt catgaagatc cgcagatccc
      121 taattttggt aaacctggcc agggacttcg attaagatcg ggcatgacct tggcgattga
      181 gccgatggtc aatatgggaa gcggtgcggt taaaattctg gaagataact ggacagcggt
      241 gacgcaggat ggaagacttt cggcccactt tgagcacacg gttcttgttg gggaaagggg
      301 cgcggagatc ctgacagaac ggccgtcttc ctgagagcag ggagagacaa atggccaagg
      361 aagatgtcgt tgaggtccag gggacaatcg ttgagacctt gcccaatgcg atgtttcggg
      421 ttgagcttga aaacggccat cgtgtcctgg cgcatatctc agggaaaatg agaatgcatt
      481 ttatcaagat tttgccagga gacaaggtta ccgtcgagct ctcgccctac gatctgacac
      541 gaggcaggat tacgtatcgt tttaagtaga ggtgaagaaa catgaaggtg cgagcttcag
      601 tcaaggctat ttgttctaaa tgcaaggtca tccgacgaaa aggtgttgtg cgtgtgattt
      661 gtgagaatca acgacacaaa caacgccagg ggtaaagcat ttcttcaggc ctgtaacagg
      721 agaataaaac gtggcacgta tcgctggaat agatctaccg cgagagaaaa ggattgaagt
      781 ggcgctgtgc tacatctacg gtatcggtcg gtcggcctca caaaagattt tgaaggaatt
      841 aaagatcagt ccggatgtcc gcgtcaagga tctcaaagag gaggacagcg tccggttaag
      901 agagaagatc gaacgggaat ataaagtgga gggtgatctt cgtaaagaag tcgctatgaa
      961 tattaagcgg ctcatggata ttggtagctt ccgcggcctc cgccatcgta aacatttgcc
     1021 tgtgagagga caacgaacca agaccaatgc cagaactcga aagggcccca cgaaggcgat
     1081 cggggtgaga cccaagaagg aacaaaccgc gtaaaagggg atccgtaggc atgaaaaagg
     1141 ggaaaaagaa agaacgcaag atcgttcaga ccggggtcgc ttatatccag gcatcgttca
     1201 acaatacgat tgtgacagtg accgatatgt ccgggaacgt cgtggtgtgg gccagtgccg
     1261 gcgggcaggg atttaaagga tcgagaaaaa gtaccccgtt tgcggcccag cgcgcggcgg
     1321 agactgccgc aaaaaaggcg atggagcatg ggatgcgcca ggtggacgtg catgtcaagg
     1381 gtcccggctc aggccgcgaa tcagcgatcc gtgccttgca agctgcggga ttgaagatca
     1441 atctgattaa agatgtgact ccgattccgc ataacggctg tcgggcgccc aaacgtagaa
     1501 gggtatagag aaggaggttt atttggcacg ttacatcggt tcagtctgtc ggctttgtcg
     1561 tcgtgaaggg atcaaactat acctcaaggg ccagcgttgt tttaccgaca aatgcgccat
     1621 tgagcgtcga a
//
LOCUS       DATMKH010000123         3970 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1341374, whole genome shotgun sequence.
ACCESSION   DATMKH010000123 DATMKH010000000
VERSION     DATMKH010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3970
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1341374"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..173
                     /gene="nrdR"
                     /locus_tag="VNV63_01525"
     CDS             <1..173
                     /gene="nrdR"
                     /locus_tag="VNV63_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017250777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HXC61329.1"
                     /translation="STNLVGEEVMGELHQMDEVAYVRFASVYREFKDLNAFMKELKQL
                     LAEDRTNRPDAV"
     gene            177..1760
                     /locus_tag="VNV63_01530"
     CDS             177..1760
                     /locus_tag="VNV63_01530"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HXC61330.1"
                     /translation="MSAPTKLELRRDCSVWGSYMWGFADVGADTYVALGLVMSYAQGA
                     TTLAFALAGCVYILIGLAYTELASAYPVAGGGPYYTLRALGDFWGFVGGSALLLDYTI
                     DIALFAAASAGYINFFLPIIVGHGIEYFAINLGPFQHVNPLWCFETMAIIGFLMFLNI
                     KGVRESSLLNEIIGVLIISWDSVIIILGFLFAWKADIFIHQWTTEFPSAHNFMYGSSL
                     AIISFVGLESISQAAQETRRPATIIPRTSIALVFTVFIFALAWSTVGSGVLPWQEFAK
                     HVDNPIALLAQSLPFIGVIAGPAAAILGALILLISANTGVMGASRLTYSLSQYGVLSD
                     RFHVVHPKYRTPVRAIMIFSLVGAAEALLAFLTPSAMDTLGNMYAFGATLGYTLVFIA
                     LLKLRFSDPYTPRPYKMPFNIKWRYKGHPVEIPVLGILGMFGVSLILFEVILTHAIGR
                     IAGPAWILLCFCYYAWYRRKAGLPLFRNGKHNWEADQMQVLEAAEEFDLLEQYKLSLA
                     ERDRQLKRETKVSSPKGWP"
     gene            1818..2387
                     /locus_tag="VNV63_01535"
     CDS             1818..2387
                     /locus_tag="VNV63_01535"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HXC61331.1"
                     /translation="MPIQINPMGLILALLFAGSMGWLLLWMLWLPVKIPAAAAKAHRS
                     VFAVKRILVPTLGQPYAERAVELACRLGQEQKAEVFLVHILEVPRTLPLGVPLPEAEA
                     EAVKVLERAESIVTLHGLKPQKIVHRARIAGEEIARIAGEQDVDMIVLGARAKVGLRD
                     EMLGRTSDLVLRLAPCEVIIDKLPDSGLS"
     gene            2403..2810
                     /locus_tag="VNV63_01540"
     CDS             2403..2810
                     /locus_tag="VNV63_01540"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HXC61332.1"
                     /translation="MRIVILGCGRLGSRLVNQLAKQGHQLVILDANEVKFKNIEDKQP
                     VQRITGNVFNEEILAKAFSEKADVFIPVTGKDNVNIMIAQIVQKKFQVPRVIVRIFDP
                     LLAEVYKGYGLETVCPTNFALDAMLNIVSKKSG"
     gene            2822..3475
                     /locus_tag="VNV63_01545"
     CDS             2822..3475
                     /locus_tag="VNV63_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HXC61333.1"
                     /translation="MYIIVVGGGKVGFNLVKLLIAEGHEVLLVEKDKLKIPNLIAEFG
                     ESVIQGNGSRVHILQEGGANRADVLVAVTGHDEDNLVICQLAKNFFKCPRTIARVNDP
                     RNEGTFSTLGIDATVSSTRLIDSLIEEQVQAKDMVIPLLTLQGGDVEIIEVELSSDSQ
                     VINKSIREIQLPDGAIFISIFRGPEVIIPKGETELKAGDRVLALVKKKVEQSLREIL"
     gene            3477..>3970
                     /locus_tag="VNV63_01550"
     CDS             3477..>3970
                     /locus_tag="VNV63_01550"
                     /inference="COORDINATES: protein motif:HMM:NF013293.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXC61334.1"
                     /translation="MAPQSITRVAIIGAGRGGISLIKIFHNDPLVRIVGVADLKSNAP
                     GIRLAKRLQIPVTNDYRKLLRSKQADLIVDVTGNKEVEEALLAFKYPGVAVIGGPSAK
                     FMWQFIDERIKNQEAIEQHLFEYQSLYSLYVKEMSLAISEERTRIALDIHDGLIQTLV
                     GLAYR"
BASE COUNT          866 a         1032 c         1087 g          985 t
ORIGIN      
        1 tctccaccaa tttggtcggg gaagaggtca tgggagaact ccatcagatg gatgaggttg
       61 cttatgtccg attcgcatcc gtttatcggg aattcaaaga cctcaacgca tttatgaagg
      121 aactaaaaca gcttctggcc gaagatcgca cgaaccggcc cgacgcggtt taaacgatga
      181 gcgcgcccac caagcttgaa ctccgacgag actgctcggt ctggggctct tacatgtggg
      241 ggtttgccga tgtcggagcg gatacctatg tcgctcttgg cctcgtaatg agctatgcgc
      301 aaggggccac cactcttgcc tttgccttag ccggctgcgt ttacatctta atcggcctcg
      361 cctataccga actggcctcg gcatatccag tcgccggcgg cggcccttat tatactctac
      421 gcgcccttgg agatttttgg gggtttgtgg gcggttcggc gcttctcctc gattacacga
      481 ttgatattgc attgtttgca gcggcctccg ccggttatat aaacttcttc ctcccaataa
      541 tcgtcggaca tggcatcgag tattttgcga taaacctggg tccgtttcaa cacgtcaacc
      601 cgctttggtg cttcgagact atggcgatca tcggcttctt aatgttttta aacatcaagg
      661 gcgtccggga atcctctctg cttaatgaaa ttatcggtgt cctgattatc tcctgggatt
      721 cggtgatcat tattctgggt tttctgtttg cctggaaagc agacatcttc attcatcagt
      781 ggacaaccga gtttccctcc gctcataact ttatgtatgg ctcctcgctt gcgatcatct
      841 cgtttgtggg actggaatct atttctcaag cggcccagga aacacgtcgt ccagccacga
      901 ttattccccg tacctcgatc gcgctggtct ttacggtttt tatttttgct ttagcctggt
      961 ccaccgttgg atcaggggtg cttccctggc aggagtttgc aaaacatgta gacaatccca
     1021 ttgcgctcct agcacagtcc cttccattca ttggagttat cgcaggtccc gcagcagcca
     1081 tattaggcgc gctgatcctt ttgatttctg caaacactgg cgtgatgggc gcttcgcgac
     1141 tgacctattc gttaagtcag tacggtgtgc tgagtgaccg gttccatgtg gttcatccta
     1201 aataccgaac gccggttcgt gcgattatga tcttctcatt ggtcggtgcg gccgaggcgc
     1261 ttttggcttt cctcacgcca agcgcaatgg acactctagg taatatgtat gcctttggcg
     1321 ccacgctagg ttacacactt gttttcattg cgctcctgaa attacgcttc tcggatccct
     1381 ataccccgcg cccctacaaa atgccgttca acatcaagtg gcggtacaaa ggccacccgg
     1441 tagaaatccc ggtgctcggt atacttggga tgtttggggt cagcctcata ttgtttgagg
     1501 tcattcttac ccacgctatc ggccgcatcg ccggcccggc ttggatactg ctctgcttct
     1561 gttattatgc ctggtatcgc cggaaagccg gcctgccgct cttccgaaac ggcaagcata
     1621 actgggaagc ggatcagatg caggttctgg aggctgcaga ggagtttgat ctcttggagc
     1681 agtacaaatt gagcctggcg gaacgggatc ggcaactcaa aagggaaacc aaggtttcat
     1741 ccccgaaggg ctggccttga catctctcct tctggtataa taatcggctc tatcgcttca
     1801 cctttaaaaa ggttgtaatg ccgattcaaa ttaatccgat gggcctcatt ctggccctgc
     1861 tctttgccgg ttccatggga tggctcttgc tatggatgct ctggctgccg gtcaagatcc
     1921 ctgccgcggc cgccaaggcg catcggtctg tctttgcggt gaagcggata ctggtgccga
     1981 cgctgggaca gccttatgcg gagcgggcgg tggaacttgc ctgtcgtcta ggtcaagagc
     2041 agaaggcgga ggtctttctg gtccatatac tggaagtacc tcgaaccttg ccgttgggtg
     2101 tcccgttgcc ggaggcagag gccgaggcgg tgaaggtcct ggagcgtgca gagtctatcg
     2161 tcacccttca cgggctcaaa ccccaaaaga tcgtccatcg ggcacggatc gcaggggaag
     2221 agatcgcccg gatcgcgggt gaacaggacg tggacatgat tgtcttaggc gctcgggcca
     2281 aggtgggcct ccgggatgag atgctgggtc gtacatctga tctggtttta cgtcttgcac
     2341 catgtgaggt cattattgac aagctcccgg actccgggct gtcttaaaga gggaggaaga
     2401 gtatgaggat tgtgatcctg ggctgcggtc ggttgggttc ccgtctggtc aatcagctgg
     2461 ctaagcaggg ccatcaactg gtcatcttgg acgcgaacga ggtcaagttt aaaaacatcg
     2521 aagacaagca acctgttcaa cggatcacgg gcaatgtctt taacgaagag atcttggcca
     2581 aggctttttc cgagaaagcg gatgtcttca tcccggtaac cggcaaagat aatgtcaata
     2641 tcatgatcgc gcagatcgtc cagaagaagt tccaggtgcc acgtgtgatc gtccggatct
     2701 ttgatccgct tctggccgag gtgtacaagg ggtacggctt ggagacggtc tgccccacga
     2761 acttcgcctt ggatgcgatg ctcaacatag tttctaagaa atcaggatag ggagaggcct
     2821 tatgtatatc atcgttgtag gtggcggcaa ggtcgggttc aacctggtca agctcttgat
     2881 cgcggaagga catgaggttc tattggtcga aaaggacaag ctgaagatcc ccaacctgat
     2941 tgcggaattc ggcgagtccg tgattcaggg aaacggctcc cgggttcaca tccttcagga
     3001 gggaggagca aatcgggcgg acgtccttgt ggcggtcacc ggtcatgatg aggacaacct
     3061 tgtcatctgc cagctcgcca aaaatttttt taagtgcccc cgcaccattg cacgagtcaa
     3121 cgaccctcga aacgagggaa ccttcagcac cttggggatt gatgccacgg tcagctcgac
     3181 ccgcctgatc gactcactga tcgaagaaca ggtccaggcc aaggacatgg tgataccgtt
     3241 gctgaccctt cagggaggag atgtcgaaat cattgaggtc gaactttcct ctgactccca
     3301 ggtaatcaac aaatccatcc gagaaatcca actgccggat ggagcgatct tcatttccat
     3361 ttttagaggc cccgaagtga tcatcccgaa aggagagacc gaattgaagg ccggtgatcg
     3421 cgtactggca ctggtcaaaa agaaggtgga gcaaagttta agagagatac tgtaggatgg
     3481 caccacagtc cattacacgt gttgcaatca tcggtgccgg acggggcggc atctcgctca
     3541 tcaagatatt tcataatgat ccgctggtcc ggatcgtcgg tgtggcggat ctcaaatcga
     3601 atgccccggg catccggctc gctaaacggc tccagatccc cgtgaccaac gattaccgga
     3661 agcttttacg atcaaagcag gccgacctga tcgttgatgt caccggaaac aaggaagtcg
     3721 aagaggccct gctcgccttc aagtaccccg gtgtcgcggt gattggaggg cccagcgcga
     3781 agttcatgtg gcaattcatc gatgagcgga tcaaaaatca ggaagcgatc gagcagcatc
     3841 tcttcgagta ccagtccctt tacagcctgt atgtcaagga gatgtctctt gcgatcagtg
     3901 aggaacgcac tcggattgcc ctcgatatcc acgacggcct catccagaca cttgtcggtc
     3961 tggcctatcg
//
LOCUS       DATMKH010000124         1049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1022523, whole genome shotgun sequence.
ACCESSION   DATMKH010000124 DATMKH010000000
VERSION     DATMKH010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1022523"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1049)
                     /locus_tag="VNV63_01555"
     CDS             complement(<1..>1049)
                     /locus_tag="VNV63_01555"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HXC61335.1"
                     /translation="ELLKKVAQQYAEADSYYIESVEERTSTTEFSHSWEKTITTAAQA
                     PGDRFHYEGHSSSGSAMKIADGKTAWIYSADGHRYTAKPQSLMTASKQIMSGTPEWAT
                     FRAENLRKNLGDMAKSLKSADRLPDATLVVNGHEVSCKVVRVQNSDRKRLSPDYTFDK
                     TVWIDKTHMTFVKTSEHAHAYLFLSSSTRVPMEEEIITVFTKAELNDSVHDELFTFIP
                     PSDAKLIQDFPDPRNNFGGTDLTGDQVPSLKLKSADGKVVSIESFRGKPVLLDFWATW
                     CGPCVEALPQLAQIYAEAKDKGFVLLAVDQDEEANTGAEFLLKKGYNWPDFHDGNGEV
                     EKLVGSSGIPRTMLV"
BASE COUNT          223 a          294 c          266 g          266 t
ORIGIN      
        1 tcgaccagca tcgtccgcgg aatcccggat gatcccacaa gcttttcaac ctccccattc
       61 ccatcgtgaa agtctggcca gttgtatcct ttcttcaaca gaaactcggc tcctgtattc
      121 gcttcctcat cttgatccac cgcaagcaaa acgaagccct tgtctttggc ctccgcatag
      181 atttgggcaa gttgcggcag ggcctcgacg cagggtccgc accacgtggc ccaaaaatcc
      241 agcaggactg gcttgccgcg aaaagactcg atcgagacga cctttccatc cgcagacttt
      301 agcttcagcg atggcacttg atcgccggtc aagtcggtgc ctccgaagtt attcctcgga
      361 tcgggaaagt cctgtatcag cttggcgtcg gatggtggga tgaacgtgaa aagttcgtca
      421 tgcaccgaat cgttcagttc agccttggtg aagaccgtga ttatctcttc ttccattgga
      481 acccgggtcg aactgctcaa aaaaaggtag gcatgagcgt gttcacttgt cttcacaaag
      541 gtcatatggg tcttgtcaat ccagaccgtc ttatcaaacg tgtaatcggg tgaaaggcgc
      601 ttccgatcgg agttctgaac ccgaacaact ttgcaggaaa cctcgtgacc gttgactacg
      661 agggtcgcat ccgggagacg gtccgctgat ttgagtgatt ttgccatatc gcccaagttc
      721 tttctaagat tctcggcccg aaacgtcgcc cactcgggcg tccctgacat aatttgtttg
      781 ctcgcagtca tgagcgattg aggcttcgct gtatatcggt gcccgtctgc actataaatc
      841 caggcggtct tcccgtctgc tatcttcatg gcactgccgc tacttgagtg cccctcgtag
      901 tgaaagcgat ctcctggagc ttgagcggca gtagtgatcg tcttctccca ggaatgagag
      961 aattcggtcg tcgaagtacg ctcttccacc gactcgatgt agtaggagtc ggcctcggca
     1021 tactgttggg ccaccttctt gagcagctc
//
LOCUS       DATMKH010000125         1003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_382545,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000125 DATMKH010000000
VERSION     DATMKH010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382545"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..>1003)
                     /locus_tag="VNV63_01560"
     CDS             complement(143..>1003)
                     /locus_tag="VNV63_01560"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HXC61336.1"
                     /translation="PTSWVLRYKAKLWNGRLLGTDLVCGSRQRGNLLHRLFELVFASD
                     PPIDWWNASRLQMDLWLEKEWHNLLPMEGANMRLPGYKVIAEGLLDEGKRAIWVLIEH
                     LRVAGVTKTSANVQTLEIPFVGGNIFGFLDLLVENASGQKAVIDLKYGGFKQKSQELS
                     DNLHLQLAVYARLVDQGSPWPEAAFLILGKRALLAQQRNFFPDADIVSSKLSPSGLQA
                     CWNEFQELWKWRRDLLDQGWIECAVSGSDGATSDLLPNSTSPIHRWQLENYADPYDDL
                     GVLTGWEENA"
BASE COUNT          249 a          289 c          247 g          218 t
ORIGIN      
        1 aattcgccag cggccttaat ggtgaaagtg gtcccgatga tggcatttgg ccggcactga
       61 ccgttgatga tactttctcg aatgacccgg gtcagctcag tagtcttgcc ggtcccggcg
      121 cccgcagcga tgctacgaac tctcacgcgt tttcctccca accggtcaga acgcccaaat
      181 cgtcgtatgg atcagcgtaa ttctccagct gccagcgatg gattggcgaa gtggagttcg
      241 gtaacagatc actggtcgca ccatcagatc cagagacagc acattcgatc caaccttggt
      301 ccagtaaatc tcggcgccat ttccaaagct cctgaaattc attccaacaa gcctggagcc
      361 ccgatggcga cagctttgat gaaactatat ccgcatcagg aaagaagttt ctctgttggg
      421 ccagtaaagc gcgttttccg aggatcaaaa aagcggcctc cggccaagga gacccttgat
      481 cgaccaagcg ggcgtaaacc gccagctgaa gatggaggtt gtcactgagt tcctggctct
      541 tctgcttgaa accgccatat ttcaaatcga tgaccgcctt ctgcccagag gcattttcaa
      601 ccagcaaatc gaggaagccg aaaatatttc cgccgacaaa gggtatctcg agtgtctgaa
      661 catttgctga tgtcttggta accccggcta cgcgcagatg ctcgatcaat acccagatgg
      721 cccgcttccc ttcatccaag aggccctccg caatgacctt gtatcctggc aaccgcatat
      781 tggcgccctc catcgggagt aagttatgcc attccttttc caaccaaaga tccatctgca
      841 agcgtgaggc attccaccaa tcaatgggag gatcagaagc gaaaaccaac tcaaacaaac
      901 gatgcaggag attgcctcgt tgacgactcc cgcagacgag atcggtcccg aggaggcgcc
      961 cattccacaa ttttgccttg taccggagca cccaggaggt ggg
//
LOCUS       DATMKH010000126         1025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2298575, whole genome shotgun sequence.
ACCESSION   DATMKH010000126 DATMKH010000000
VERSION     DATMKH010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2298575"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            33..>1025
                     /locus_tag="VNV63_01565"
     CDS             33..>1025
                     /locus_tag="VNV63_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61337.1"
                     /translation="MEREKGRVRALVLKARQMGSTTYNSAEFFHKTIFAEHVTSIIVG
                     QDEDQSTFIMSMYESALDFIPWWMRPRIKQKQTGALINFDEKDEVLRQTRPGLKTWVY
                     ADNGRKPSGVGRGKTFGRALLSELAFWQDLSQLSKSLFPTFNTPDGFYIMESTANGRN
                     DAWHNLWRRAEAGKVDWEPIFIPFYRREKTYSTPILKTEVFVLTKDEIEMKERVFKKE
                     SYLIKPEMFNWMRKKKEEFIATDGDDMMFAQEYTSEPEESFQSSAITAFPRGVINRHS
                     KRTRNPVWLGEIYYDFQLGKAVPVLEEIDETFELPYPEQEKRFSVWERPVRGERY"
BASE COUNT          271 a          236 c          297 g          221 t
ORIGIN      
        1 cgcaggagat gctttgcgag agataccgga agttggagag ggagaaaggt cgcgtaaggg
       61 ctctggttct taaagcccgg cagatgggtt ctacgactta caactccgcc gagttctttc
      121 acaaaaccat ctttgccgag cacgtaacct cgattatcgt aggccaggat gaagaccagt
      181 cgacattcat catgagcatg tacgagtcgg ctttggactt tatcccgtgg tggatgcgcc
      241 cacgtatcaa gcagaaacag actggggctc ttatcaactt cgacgagaag gatgaagtac
      301 tccgtcagac ccgccccgga ttgaagacct gggtgtacgc cgataacgga cggaagccat
      361 cgggtgtcgg ccgcggaaag acattcggac gggctctcct gtcggagctt gctttctggc
      421 aggacttatc tcagttgtcg aagtctctgt tcccgacgtt caacactccg gatggtttct
      481 acataatgga gtcaacagcc aacggaagga acgatgcatg gcataacctg tggcgccggg
      541 ctgaggctgg caaggttgac tgggagccta tctttattcc gttctaccgc cgcgagaaga
      601 catactcaac tccgatcctg aagacggaag ttttcgttct cacgaaggac gagatcgaaa
      661 tgaaggagcg ggtattcaag aaggaaagct atctcataaa gccagagatg ttcaactgga
      721 tgcgcaaaaa gaaagaagag ttcatcgcaa cagatggcga cgacatgatg tttgctcaag
      781 agtacacgtc agagcccgaa gaaagcttcc agagttccgc tatcacggct ttcccaagag
      841 gagtgatcaa taggcatagc aagcgcacta ggaatccggt gtggcttggg gagatttact
      901 acgacttcca gttggggaag gccgttccag ttcttgagga aattgacgag acgtttgaat
      961 tgccgtaccc ggaacaggaa aaaagattca gtgtttggga aaggccagtg cggggggagc
     1021 ggtat
//
LOCUS       DATMKH010000127         1042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1663797, whole genome shotgun sequence.
ACCESSION   DATMKH010000127 DATMKH010000000
VERSION     DATMKH010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1663797"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="VNV63_01570"
     CDS             complement(<1..478)
                     /locus_tag="VNV63_01570"
                     /inference="COORDINATES: protein motif:HMM:NF015747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HXC61338.1"
                     /translation="MKESSMTKLKKICFIGAGNMVEAMIQGILAAGLFRRQEMLASDA
                     SKERLEYMREKYGIKVTGNNKNAVEWADLVMLAVKPQVMDIVLEDIRKAVDDSKLLIS
                     IAAGIPISKIFDGLTKEAKVIRVMPNTPALVKEGASAIAPGRGVLQPELTLVKKIFE"
     gene            complement(475..>1042)
                     /locus_tag="VNV63_01575"
     CDS             complement(475..>1042)
                     /locus_tag="VNV63_01575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HXC61339.1"
                     /translation="REVLAAGATVLGENRVQEALPKIHSLEKAVKWHFVGHLQTNKVK
                     QIIGMFELIHSVDSLRLAQEISRWAERLGICQPILVQVNLSREASKFGLAPEEVKPII
                     QEIAGLPGIAIQGLMTILPLSSDPEAGRVYFRRLRVLASEVSRWGFSGVTMGELSMGM
                     SDDFEVAIEEGATWIRIGTAIFGPRSDR"
BASE COUNT          246 a          314 c          230 g          252 t
ORIGIN      
        1 cctcaaagat cttttttacc agagtaagtt ccggttgcag aaccccccgg cccggagcga
       61 tggctgaggc gccttcctta accaaggctg gggtattggg catcacacgg atcaccttgg
      121 cttctttggt cagaccatca aagatcttgg agatcgggat ccccgctgcg atcgaaatga
      181 ggagtttaga atcatccacc gccttgcgga tatcttccag cacgatatcc atcacttgag
      241 gtttgacggc aagcatcacg agatcagccc actcaaccgc attcttatta ttgccggtta
      301 ccttaatacc atacttctcc ctcatatatt ccaagcgttc cttggacgcg tcagaggcca
      361 gcatctcttg gcgtctgaaa agccccgcgg cgaggatccc ttggatcatg gcctccacca
      421 tattaccggc accaatgaaa cagatcttct taagcttggt catggatgac tccttcatct
      481 gtcgctcctt ggcccaaaga tggccgttcc tattcttatc catgtagccc cttcttcaat
      541 ggctacctca aaatcatctg acattcccat cgagagttct cccatcgtca cacccgagaa
      601 cccccatcga gaaacctcag aagccaaaac ccgcaagcgg cgaaagtaaa cgcggccggc
      661 ctcaggatcg cttgataagg gtaaaatcgt catgagacct tggatcgcaa ttccaggaag
      721 tcctgctatc tcttggataa tcggcttcac ctcttcaggc gccaggccga atttcgatgc
      781 ttccctggaa aggttcactt gaaccagaat aggttgacag atccccaacc gttcagccca
      841 tcttgagatc tcctgggcga ggcgaaggct gtctaccgaa tggatcagtt caaacattcc
      901 gattatttgc ttgaccttgt tagtctggag atgccctaca aaatgccact tcaccgcctt
      961 ttccagagaa tgaatcttgg gcagcgcctc ctgcacgcgg ttctcaccca agacggtcgc
     1021 cccggccgca agcacttccc ga
//
LOCUS       DATMKH010000128         1063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3584441, whole genome shotgun sequence.
ACCESSION   DATMKH010000128 DATMKH010000000
VERSION     DATMKH010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3584441"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="VNV63_01580"
     CDS             <1..289
                     /locus_tag="VNV63_01580"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HXC61340.1"
                     /translation="DYDFNVVATFEEFPGKEPSPRAIERLRKTIVDGNVCALFSEPQY
                     SPKAMQIIGKEFKVPVVQLDPMETGDGSKDFYERVTRKNMDALVEAFHGGR"
     gene            276..1031
                     /locus_tag="VNV63_01585"
     CDS             276..1031
                     /locus_tag="VNV63_01585"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HXC61341.1"
                     /translation="MEADRKSYLSVENLSVSYGQHHVLEDISFEIERGQFVCVIGPNG
                     GGKSTLLKALVGLLPIRSGIMRKEAKNIGYVPQQLRIDPAVPLRVDEFLSLKLSKRRV
                     WFGPGRTRATARKKLEEIGAAHLIDRQIGELSGGEFQRILIAYALLDNPDLLLLDEPM
                     TGLDVRGGMSFDGLLHHLNEHAGITIVMVSHDLHLVEHLSDQVLCINRTVHCHGSPDQ
                     VLQPDNLSKAYGHLPGLVSGHGIEAFIPLGKLK"
BASE COUNT          254 a          257 c          303 g          249 t
ORIGIN      
        1 ggattatgac tttaatgtgg tggcgacgtt cgaagaattc ccgggcaagg agccttcgcc
       61 gagagcgatc gagcgattgc ggaagacgat cgtcgacggg aatgtctgcg cgcttttttc
      121 cgaaccgcaa tactcgccga aggccatgca gatcattggc aaagaattca aggtacccgt
      181 tgtgcagctc gatccgatgg aaaccggaga cggatcgaag gacttctatg aacgcgtgac
      241 acgaaagaat atggatgcgt tagtagaggc gtttcatgga ggccgataga aaatcctacc
      301 ttagcgtgga gaacttgtcc gtgagctacg gtcaacacca cgtcctcgaa gatatttcat
      361 ttgagatcga acgcggtcag tttgtttgtg taatcggccc gaacggaggg ggaaaatcca
      421 cgctcttgaa ggcactggtc ggtctcttgc cgatccgctc cgggatcatg cgtaaagaag
      481 ctaaaaatat cgggtatgtc cctcaacagc tgcgaattga cccggctgtg ccgttgcgcg
      541 tagatgaatt tttgtcactc aaactctcga aacgaagagt ctggttcgga cccggtcgaa
      601 cgcgggcgac ggctcggaaa aaactcgaag agatcggtgc tgcacacttg atcgatcggc
      661 agatcggtga attgtccggc ggagaattcc agcgaatttt gatcgcctac gctctcctgg
      721 ataatcctga tttgcttctt ttggacgaac cgatgaccgg actcgacgtg cgaggcggga
      781 tgtcctttga cggtttgctt catcatctca atgagcacgc cgggataacg atcgtcatgg
      841 tctcacacga tctgcatctg gtggaacacc tcagcgacca ggtcttgtgc atcaatcgga
      901 cggtccattg tcacggatct ccggatcagg tgctgcagcc ggacaacctc tcaaaggctt
      961 atggacatct ccctgggttg gtctccgggc atggaatcga agcatttatt ccgttaggga
     1021 aattgaaata acccgaggtg tgcttgagac atgtgggaga tcc
//
LOCUS       DATMKH010000129         1070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4543566, whole genome shotgun sequence.
ACCESSION   DATMKH010000129 DATMKH010000000
VERSION     DATMKH010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4543566"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1028)
                     /locus_tag="VNV63_01590"
     CDS             complement(<1..1028)
                     /locus_tag="VNV63_01590"
                     /inference="COORDINATES: protein motif:HMM:NF013755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HXC61342.1"
                     /translation="MTLVTGDKDMFQLVSPRIKIYDTMKEKVYRVEEIKARFGVGPSQ
                     MIEIMGLMGDTSDNIPGVPGIGEKTAIKLIGEFGDLEHLLSRLDEVKQPKLRENLSKF
                     SEQARLSRRLATIRTDCPMGFSLSDYRLKGPEMDKLIPLFKELGFESLTKTLSQNAQQ
                     DHLNQIKPVGAYHDTPPLAGTSAAESVMLPDSDSPQRPPALIILDEAGLDRLVQRLES
                     ATSVALALQAAPPDSVRGQLQGLSLAATEQEAFYIPLDQYSSSLAEEKVLAALKPILE
                     NLKIKKYGHDLKRAFIIFKRHGVQLQPISFDTMIASYLLTPLRRGHGLDTIVLEYLQH
                     SLPPVPQPP"
BASE COUNT          241 a          273 c          292 g          264 t
ORIGIN      
        1 ggaggttgcg gaacgggagg cagtgaatgc tggagatatt ccagaacgat ggtgtccaga
       61 ccgtggccgc ggcggagagg agttaaaagg taagaggcga tcatcgtgtc aaaagagatc
      121 ggttgaagct gaacaccgtg ccgcttgaaa ataataaagg cacgttttag atcgtgaccg
      181 tattttttga tcttcaaatt ctccagaatt ggtttgagcg cggctagcac cttttcctca
      241 gccagcgatg aagaatattg atcaagcggg atgtagaatg cctcctgctc agttgcagca
      301 agagataatc cttgaagctg accgcgcacc gagtcggggg gggcggcttg aagagcaaga
      361 gcgacgcttg ttgccgattc taatcgctgg acaagacggt cgagccctgc ttcatcaagt
      421 attattagag cagggggcct ctgcggtgaa tcggaatccg gcagcatcac cgactcggcg
      481 gcgctcgtgc cggccagtgg gggcgtatcg tgatacgccc ctactggctt aatttggttc
      541 agatggtcct gctgggcatt ctgtgaaaga gtttttgtga ggctttcgaa gcccagttct
      601 ttgaacagag ggatcaactt atccatttcc ggccctttta agcggtagtc cgataagctg
      661 aaacccattg ggcagtccgt ccggatagtg gccaggcgtc gggaaagcct tgcctgctcg
      721 gaaaacttgg ataaattctc tcgcagcttt ggctgcttca cttcatccaa gcgagagagc
      781 agatgctcca gatcaccgaa ctccccgatc agcttgatgg ctgtcttttc tccgataccg
      841 gggacgcccg gtatattatc gctggtatct cccatcagac ccatgatctc gatcatctgg
      901 gacggtccca cgccaaaacg ggccttgatc tcttccaccc gatatacctt ttccttcatc
      961 gtgtcataaa tcttaatgcg agggctgact aattggaaca tgtccttatc ccccgtgacc
     1021 agagtcacct caagtccttc cgcttcggcc ttcttggcga tcgtaccgat
//
LOCUS       DATMKH010000130         1999 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3907785, whole genome shotgun sequence.
ACCESSION   DATMKH010000130 DATMKH010000000
VERSION     DATMKH010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3907785"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            352..696
                     /locus_tag="VNV63_01595"
     CDS             352..696
                     /locus_tag="VNV63_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61343.1"
                     /translation="MSTIHLKIEEFHRIWLKPCDPARFFLVVEPAPERDYAYVRFADL
                     EVGDRILYGGDYDEEMKQEIQVLRPRTILEIREQAAEAGQPERELILLAQNGKIYTFD
                     FYANQRVYTALK"
     gene            860..1750
                     /locus_tag="VNV63_01600"
     CDS             860..1750
                     /locus_tag="VNV63_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61344.1"
                     /translation="MYDLNDHGMPDFPVLEFPKRTTGELGEKIFRVIMLTDPLALTGW
                     FCVPTFQYQEQAQKYIDRMHAAIELHPITDWNVSFAIIPPEQMAHISKIEPLFVHPCQ
                     RVYLRDIEVWMHLFVGDRFSPIAWCQGAQGVAYCAAQIPWPESIENDDGWIWSMVRSY
                     PPGMTMERFASMVEPHGELQPVMDLSRIHPTMNPVERKIIAQILSDAKIAGCNIDVFD
                     GEEFCLVHADNPQADMGKVFSEMFGSDEDYLTFYQGENEIGVVWLIYGNKGCLISDHT
                     DNPAMEALLKAAFEIAEKYQ"
     gene            1812..>1999
                     /locus_tag="VNV63_01605"
     CDS             1812..>1999
                     /locus_tag="VNV63_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61345.1"
                     /translation="MSDLLQPYILDPFEGEGKLIDNIEGAPVYYLAFSRLGLMEYDLT
                     YCDGCANTPALRASIQEGR"
BASE COUNT          517 a          453 c          544 g          485 t
ORIGIN      
        1 ccccgcctac ggaagttaaa acgctcataa tcgatttcgc ggcagaggcg agcgttcttg
       61 cccgcctgaa ggcgcggggc gcaaccatcc atcaatatat ccaatgcctg tctccgcgca
      121 ggcgctcgaa gatctgctat accattatta gccgatagag aggaaaaagg aatgaaaaga
      181 cgagcgactt tattctcgca gctatgtgat ctgcggccag gggatcatat cataatctgg
      241 cgagatcagt tagatattcc acctcggcag agtcgcatac atgaggataa tcccggtcat
      301 ctttatgagg cggaggtatg tagtaaagcc gttgaccctc ccataccagg agtgtcaacc
      361 atccatctca aaatagagga gtttcatagg atctggctca agccttgtga tccggctcgt
      421 ttcttcctag tcgtagagcc agcgccagag agagactatg cctatgtgcg cttcgccgat
      481 ttagaggtcg gcgatcgtat cctctatgga ggcgattatg atgaggagat gaaacaggag
      541 attcaagtac tccggccacg aaccattctc gaaatacgtg agcaggcagc agaagcaggc
      601 cagccagagc gcgaactgat tctgctggcg cagaatggga agatatacac ctttgatttc
      661 tatgcgaatc aacgagttta tactgcgctg aaatagccca cagcaaaaga ggcaagatac
      721 tttaaacaca agtattcttg cctcttcttt tatttacctt tgatttgcat tcctgctatg
      781 aatatgctat aatgagagag atggcgcagg caagcgagcc tgggccgagc gattgaaaag
      841 caaaggggaa gtatcttcta tgtatgatct caatgaccac ggcatgccgg attttccggt
      901 gttggaattt ccgaagcgca caactggcga acttggcgag aagatattca gggttatcat
      961 gctcactgat ccattggcat tgaccgggtg gttctgcgtg ccaactttcc aatatcagga
     1021 gcaagctcag aagtatattg atcggatgca tgcggccatt gagttgcatc cgattactga
     1081 ctggaatgtc agctttgcga taattcctcc tgagcagatg gcgcatatca gcaagatcga
     1141 gccattgttc gtacatccct gccagcgcgt ctatttgcgg gatatagaag tctggatgca
     1201 tctcttcgtc ggcgatagat tttcgcctat cgcctggtgc cagggggcgc agggagtggc
     1261 ctattgcgcc gcgcagatcc cctggccaga gtcgattgaa aatgatgatg gatggatctg
     1321 gtcaatggtc cgatcctatc cgccaggaat gaccatggag cgctttgcga gcatggttga
     1381 gccgcatggg gaattgcagc cagttatgga cctgagccgg attcatccaa cgatgaatcc
     1441 agtcgagaga aaaatcatcg cgcagatcct atcagatgcg aagatcgccg ggtgcaatat
     1501 cgatgtcttc gatggggaag aattctgcct ggttcatgcg gataatccgc aggcggatat
     1561 ggggaaagtc ttttcggaaa tgttcggttc tgatgaggat tatctgacat tctatcaggg
     1621 cgaaaatgag ataggagttg tctggttgat ctatgggaat aaggggtgtc tcatttccga
     1681 tcatactgat aatccagcga tggaagcact tctgaaggct gctttcgaga tagccgagaa
     1741 atatcaatag catgtgcgag aggggtcagt cggagcgatc tggctggccc agaaacgaga
     1801 gagaaagaat catgagcgat cttttacaac cctatattct cgatccattc gagggcgagg
     1861 gcaaattgat tgacaacatt gaaggagcgc cagtttacta tctggcattc tccaggttgg
     1921 gcctgatgga atatgatctg acctattgcg atgggtgcgc caatacgccc gcattgcgcg
     1981 ccagcatcca agagggacg
//
LOCUS       DATMKH010000131         5457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_386148,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000131 DATMKH010000000
VERSION     DATMKH010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 5457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5457
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386148"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="VNV63_01610"
     CDS             complement(<1..362)
                     /locus_tag="VNV63_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXC61346.1"
                     /translation="MRLENKTVLITGAGSGIGRETALLFSSEGAGIVAVDVDDAGGKE
                     TVKMIEAQGARAVYVHADVAKGSDCEMMVAAAEKNFGKLNVLFNNAGIMDGRDDDAVK
                     TEEDVWERTISVNLKGVFL"
     gene            complement(377..1759)
                     /locus_tag="VNV63_01615"
     CDS             complement(377..1759)
                     /locus_tag="VNV63_01615"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015814112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HXC61347.1"
                     /translation="MGSKVTGMMTQDELARRVSAGEIDTVLLVFTDHYGRFMGKRLDA
                     DFFLEDAAKPGTHACDYLLTVDMEMEPVPGYRFANWEKGYGDFHLVPDFATLRIASWL
                     EKTALLICDLEDEKNHRPVSQAPRSILRNQVERAAKMGYRAMAGSELEYYIFKNSYRD
                     AATKGYHGLEPAGWYLEDYHVLQGTREEAFNGAARRHLKQSGIPVESSKGEWGLGQHE
                     LNVRYADILTMADRHAVFKQCLKEIAEQTGISVTFMAKFAANQAGSSCHIHMSLWRKN
                     QSAFPGKGRLGPVACSETFRWFLGGWITHVPEMMVFYAPTVNSYKRYQAASWAPTRLA
                     WSYDNRTAGFRVVGEGDSLRIECRIPGADCNPYLAFSAALASGLDGIENKIEPPQIFE
                     GDVYAARHLPLVPRTLRDATDLFQKSDFAKSAFGEDVVEHYLHFYRMEQEGYDKAVTD
                     WERQRYFERI"
     gene            2070..2522
                     /gene="msrB"
                     /locus_tag="VNV63_01620"
     CDS             2070..2522
                     /gene="msrB"
                     /locus_tag="VNV63_01620"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HXC61348.1"
                     /translation="MLTRGFGLLPVWAAETKKTFEVVKTDEEWRRILTPEQYRVLRQE
                     STEPPFQNKYHDYKEKGTYHCAGCDQPLFSWRHKFDSGTGWPSFWQPMFSDAVGTKID
                     RKLAYPRTEVHCARCSGHLGHIFNDGPAPTYLRYCINSAALIFKPATG"
     gene            2740..3936
                     /locus_tag="VNV63_01625"
     CDS             2740..3936
                     /locus_tag="VNV63_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61349.1"
                     /translation="MIRSRFRNALVWIALLVGTPLFSELAQGEGPSDLESICRDEVKR
                     DCSFVQPSTGDNASDSKNSALFRCLLKHESDASPACREQLKNVPVAELPKLPPKAVPG
                     FAALGILGLLPATTPTVSYGGWVTPEQGSRGVEYNRLTIQVPVYQDDKDTFSMSAGGT
                     SLHFGEQHTLSGSGIPVPEDLWKVEFGGSYSRKLEDDKLIGGRISFGSASDHPFADFD
                     VITIGASAFYSWASTERSRWMLTILFSNNNPIINYVPIPGFIYMYWTPNFVGMFGFPF
                     SSIVWTPLEPWMFTLSVFGPTINSEIAYGNPKKIQFFTGFSWLQQSYLRENRPDPKDR
                     LYFDEKRVPLGMRFPVAKALKSELSVGYAFDRSVYEGTRFGNKDDGAASLGNSWYAAW
                     NLRLEF"
     gene            complement(4081..4407)
                     /locus_tag="VNV63_01630"
     CDS             complement(4081..4407)
                     /locus_tag="VNV63_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61350.1"
                     /translation="MLDAIGLLFFIIPGVIAFAVDFSTGAIYLPSYSRHSRRHARAQN
                     GMEKDALRIVKIDPKRLDRDTLRQIITEQTGYHIRLNDTNLIVIRADEGVDIDRELIR
                     LSSEIQ"
     gene            complement(4655..5137)
                     /locus_tag="VNV63_01635"
     CDS             complement(4655..5137)
                     /locus_tag="VNV63_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61351.1"
                     /translation="MRRILIVLAIFGAMAIAAPSSYAGVGVNINIGVPLPPPVVVQAP
                     PEMVIIPEAGVYVAVGTPYDLFFLSGRYYYFHNGYWYGARGYGGPWVHVENRALPGGL
                     RKYKIERLHEFREYASKDYRERGPAYKGKHFRADEREIAKDRHESTRERGGPDRHGER
                     "
BASE COUNT         1262 a         1516 c         1349 g         1330 t
ORIGIN      
        1 agaaagaccc ccttgaggtt gaccgatatg gtccgctccc acacatcttc ttcggtcttg
       61 acggcgtcat cgtcgcggcc gtccatgatt ccggcgttgt tgaagaggac attcaatttt
      121 ccgaagtttt tctccgccgc cgcgaccatc atctcgcaat cgctcccttt ggcgacatcg
      181 gcatgaacat aaacggcgcg cgcgccctgc gcctcgatca tcttcacggt ctcttttcca
      241 ccggcgtcgt cgacatcgac cgccacgatc ccggcccctt cactcgaaaa gaggagcgcc
      301 gtttctcgtc cgatcccgct tcccgccccg gtgatgagga ccgttttatt ttccagccgc
      361 atccggttct ccccttctaa attctctcga agtatctctg acgctcccag tcggtcaccg
      421 ctttatcata tccctcctgc tccatcctgt aaaagtgaag atagtgctcc accacatctt
      481 cgccgaacgc gctctttgca aaatcgcttt tttggaagag atcggttgca tcgcgaagcg
      541 tgcggggaac aagaggaagg tgccgcgcgg cgtagacgtc tccctcaaaa atttgcggcg
      601 gctcaatttt attttcaatg ccatccaggc cggaggcgag ggcggccgaa aaggcgaggt
      661 aaggattaca atcagcgcct gggatgcggc actcgatccg caagctgtct ccctccccca
      721 ccacgcgaaa accggcggtg cggttatcat agctccaggc aaggcgggtc ggcgcccatg
      781 aagcagcttg gtatcgtttg tacgaattaa cggttggggc gtagaagacc atcatctccg
      841 gcacgtgagt gatccagccg ccgagaaacc agcggaacgt ttcggagcag gcgaccggcc
      901 caagacgtcc ctttccggga aaggcactct gatttttccg ccagaggctc atatgtatgt
      961 ggcagctcga tcctgcctga ttggcggcga acttggccat gaaagtgacg ctgatccccg
     1021 tctgctccgc gatctccttc aaacattgct tgaagaccgc atggcggtca gccatcgtga
     1081 gaatatcggc gtagcggaca ttcaactcgt gttgccccaa tccccactcc cctttcgagc
     1141 tctcgaccgg aattcccgac tgcttgaggt ggcgtcttgc cgcgccgttg aacgcttctt
     1201 cacgagtgcc ttgcaataca tgatagtcct cgagatacca gcccgcgggt tcaaggccgt
     1261 gatacccttt ggttgccgcg tcgcgatagg aattcttaaa gatgtaatac tccagctccg
     1321 atccggccat cgcgcggtac cccatcttgg cggcccgctc tacctgattc ctcaaaatcg
     1381 aacgaggcgc ctgcgatacc gggcgatgat tcttttcgtc ctctaggtcg cagatcagta
     1441 gcgccgtctt ctccaaccag gaagcgatcc ggagagtcgc aaaatcagga accagatgaa
     1501 aatctccgta gcctttctcc caattggcga accgataccc cggcaccggc tccatctcca
     1561 tatctacggt gagcagataa tcacacgcgt gtgtccccgg cttggccgca tcctccagga
     1621 agaaatcggc atcgagccgc ttccccatga agcgcccgta atgatcggta aagacgagca
     1681 ggactgtatc aatttccccc gcgctgaccc gccgggccag ctcatcctgt gtcatcatgc
     1741 cggtaacctt ggagcccatt ggagaacctc ctttatatgt gagggccctt gcggcaaaag
     1801 aaggaacagg tcctccgatg cccccacacc ccgcggcttg cactggcata gccagatgcg
     1861 cgccttggat cttcaattcg aatacgtctt taataatacc ccattgaggt aaacgtggga
     1921 agggaatttt ataaggcgca cattgacaag atctatggta tcgtcctaaa attagacaag
     1981 aggccatgga caaacttacg gcattgttaa tagaattatc tatcacacgc cgatcgttgc
     2041 tcaagggggc ggcaatcctg ggagccggca tgctcaccag aggctttggc ctgctgccgg
     2101 tctgggcggc ggagacaaaa aagacatttg aagtggtaaa gacggatgag gagtggcgga
     2161 gaattctcac cccggagcag tatcgcgtcc ttcggcagga gtcgacggaa ccgccgttcc
     2221 aaaacaaata ccacgactat aaagagaaag gcacttacca ttgcgccggc tgtgatcagc
     2281 ccctcttttc atggcggcac aagtttgaca gcgggaccgg atggcccagc ttttggcagc
     2341 cgatgttttc cgatgcggtc ggcacaaaga tcgaccggaa gctggcctat ccgcggactg
     2401 aggtgcattg cgcccgttgc agcggccatc tgggccacat ttttaacgat ggtccggcgc
     2461 caacctatct gcgctactgc atcaattcgg ccgccctgat cttcaagcct gctacgggat
     2521 gatccaggcc tgcctccaat aaatcagttc agggtctgag ggttcccttg atataattct
     2581 gagacacgat acttattgcg gtgatcgggg aattaattag ttcaacactg ctcacccttg
     2641 ttgttgttcc cgcggattac tcgtatatcc atcgatttgg aaaatgggtc atgcgggctt
     2701 tcaactgtat tgtgagtcac gaggaggagc ggaaaattga tgatccgttc tcggtttaga
     2761 aacgccttag tgtggatagc tcttttggta ggaacgccgc ttttctcaga acttgctcaa
     2821 ggggagggcc cttccgacct tgaaagcatt tgccgggatg aggtgaagcg ggactgctca
     2881 ttcgtacaac catcaacagg cgacaatgcc tcagactcga aaaactccgc tctttttagg
     2941 tgcctgctca aacatgaaag cgacgcttct cctgcttgca gggagcaact caaaaacgtg
     3001 cctgtcgcgg aattaccgaa gcttccccca aaagcggtgc ctggtttcgc tgcgcttggc
     3061 attctaggtc tgcttcctgc cacaacgcca acggtatctt acggcggttg ggtcacaccc
     3121 gagcagggtt ctagaggtgt cgagtacaat cgcttgacga ttcaggtacc ggtttatcag
     3181 gacgacaagg ataccttttc gatgtcggca ggaggcacgt cacttcattt tggagagcaa
     3241 cacacgctct ctggctcagg gatcccagtc ccggaggatc tttggaaagt ggagttcgga
     3301 ggatcttatt ctcgcaagct tgaagacgac aagctcatcg gtggacggat cagctttggg
     3361 tcagcgagcg atcatccctt tgccgatttt gatgtgataa ccatcggagc cagtgcattt
     3421 tatagttggg caagcactga aagaagccga tggatgctga cgattctttt ctcgaacaat
     3481 aacccaatta tcaactacgt tccgatccca ggcttcattt acatgtactg gaccccaaac
     3541 tttgtgggca tgttcggttt ccctttcagt tcgatcgttt ggacgccttt ggaaccttgg
     3601 atgttcactc tttcggtttt tggccccacg atcaactcag aaatcgctta tggtaatcca
     3661 aaaaaaattc aattcttcac cggatttagc tggctccaac aatcttatct tcgagagaat
     3721 agacccgatc cgaaagaccg tttgtacttt gatgagaagc gtgtcccgct cgggatgagg
     3781 tttccggtgg caaaggctct caagagcgaa ctttctgtgg gatacgcttt tgatcgttcg
     3841 gtttacgagg gaacccgttt tggcaacaaa gatgatggcg ccgcgtccct tggaaactcc
     3901 tggtatgccg cgtggaatct tcgtcttgag ttttgaatcg cgctctcaaa aagagaatgc
     3961 gtctacccaa tgattttgta gatatcccgg tgcaagcgcg gggaactcaa agcggccatt
     4021 aaaaggtgaa ccttgaggcg aggactcaac cttcaaaatc tattttggac aagcatagtt
     4081 ctactgtatt tctgagctca accggatcag ttccctgtcg atatctactc cctcatccgc
     4141 cctgatcaca atcagattgg tatcgttcag ccgaatgtga tatccggttt gttcggttat
     4201 aatctgtctg agtgtgtccc gatcaagtct tttcggatca atttttacaa tgcgcaatgc
     4261 atctttctcc attccgtttt gcgctctggc atgtcttctt gaatgccggc tgtatgatgg
     4321 caagtaaatg gcaccggtac tgaagtctac cgcaaaggcg atgacacccg gtatgatgaa
     4381 aaagagaagt ccaatcgcat ccaacaaaac tacaccaaca tcggcctgac catattgttg
     4441 gccccgcctc tccggataca gcagcgttcc gcaagaagca agctgtaaca gaaggagtga
     4501 acacaacaga acggatatag acttctttat gctttattcc ttcctccaag aaaattcatg
     4561 ttttggagca gaacaatccg cagatcacaa agccttgagc gcagcacatt aatcatcaaa
     4621 aggccgcccg cacccgcttc ggccattata acttttatct ctccccgtgt ctgtccggcc
     4681 cgccgcgctc tctcgtgctc tcgtgccgat ccttagcgat ttctctttca tcggctctaa
     4741 aatgctttcc tttgtaagcc ggacccctct cacggtaatc cttcgaggca tactcacgga
     4801 attcatgcag ccgctcaatc ttatattttc ttaaaccgcc gggaagcgct cgattctcga
     4861 catgaaccca cggaccgcca tagccgcgag ccccgtacca gtagccatta tgaaaatagt
     4921 agtatcggcc actgaggaaa aaaagatcgt agggggttcc aaccgctacg tagactccgg
     4981 cctccggtat gatcaccatc tcgggcggtg cttgcaccac gactggcggc ggtagtggaa
     5041 caccgatatt gatattcaca cccacgcccg cataacttga cggcgcggcg atggccattg
     5101 ctccgaatat tgccaggacg atcagtattc ttctcatggt tcctccttca ttctgatttt
     5161 taaatgatta caatttcttg aaatagccga aaaagctgga cctctcaaat cgatgtctgg
     5221 cgaccggtgc caaccgcgat tagagagagg cggccgtaaa cgacccaaat ggaacatgca
     5281 cctgaggccc ggaaagtgtc gtgcacacgg tcatccagac caccagcgct ccaaccattg
     5341 caaggttctc ccagttcatt tacggattag agaatcactt gatttgagga tacaccggtt
     5401 cctaaaaaaa gtctggtcaa tattagacat atctctattt cgggaggttt ttcggtt
//
LOCUS       DATMKH010000132         4419 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1665554, whole genome shotgun sequence.
ACCESSION   DATMKH010000132 DATMKH010000000
VERSION     DATMKH010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4419
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1665554"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="VNV63_01640"
     CDS             complement(<1..184)
                     /locus_tag="VNV63_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitropropane dioxygenase"
                     /protein_id="HXC61352.1"
                     /translation="MSDEEKLNVTCSCCGATLLVDRQTGQVIWHEAKGKKVEHKTMKE
                     MVDVLEVQRRETAEKFE"
     gene            322..954
                     /locus_tag="VNV63_01645"
     CDS             322..954
                     /locus_tag="VNV63_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61353.1"
                     /translation="MKPVWTISFAVLITIIFLINSARAMLMDRILAVVNRQPVTLSDL
                     HRYEAFLALSDGKKNWEPPPSPSPTMLHQLIDQKLLLEEAEKFEVSPSKTTDLLEAQD
                     RLAKRFTNPEEFQNTLLRWGLDRESLHHEIEDHLMVERFIDQRITFFVIIPTAEIQKY
                     YEAHREEYREKRLEEALAHITRQLTDLKAKEKLESYLKKLREKASIQINS"
     gene            complement(957..1424)
                     /locus_tag="VNV63_01650"
     CDS             complement(957..1424)
                     /locus_tag="VNV63_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014652742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HXC61354.1"
                     /translation="MSKSPEEILKELGLNLPDAPKPLGAYVPAVRAGNLLFLSGMLPL
                     ILGELQYQGKLGMDLTIEQGYESARTAVLNALAVIRQEIGDLSRVARVVRLTGYVTSA
                     PGFTQQAAVLNGASDLLLKMFGERGKHARLALGAAELPLSSPIELELIISLEN"
     gene            complement(1425..2474)
                     /gene="mltG"
                     /locus_tag="VNV63_01655"
     CDS             complement(1425..2474)
                     /gene="mltG"
                     /locus_tag="VNV63_01655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HXC61355.1"
                     /translation="MQKGGSKTLSRFLIPVFLVGSLIFFLVVHFLTFLYIPSSKEEVN
                     KVIEVQAGATLRDVATLLSRESLITRVPYFILLGKLTLAERRIKPGEYAFTTRMTPLE
                     IMDALKKGRVLTYELVVPEGYTADQIAHLVEDLKMADYDTFMVLVHDPDFIRSLGLKT
                     DGLEGYLFPDTYYFPKRIGAEEVIKTMVAKFKNTFEPELETRAAELNMTQNEVITLAS
                     IIEKETSVEYERRLVSAVFHNRLKRNIPLQSDPTVIYALLPDFHGTLHKKQLKVKSPY
                     NTYQNKGLPPGPIASPGRESLYAALYPDNVDYLYFVSKNDGTHYFSATLREHNRAVQK
                     YQKRKGGKTLVRENS"
     gene            complement(2474..2932)
                     /gene="ruvX"
                     /locus_tag="VNV63_01660"
     CDS             complement(2474..2932)
                     /gene="ruvX"
                     /locus_tag="VNV63_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010347259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HXC61356.1"
                     /translation="MRILALDVGDRRIGLAISDEMGWTAQALETLIRKDLKRDLLYLT
                     ELILSREVTEVVVGLPRHLDGHIGPQAQKVLTFVESLKTRMPVPVLMWDERLTSREAE
                     RTLIEAHVRRSKRKTVVDQMAAVLILQNYLDAHQVRLSGRQTASHPIGEV"
     gene            complement(2937..3239)
                     /locus_tag="VNV63_01665"
     CDS             complement(2937..3239)
                     /locus_tag="VNV63_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61357.1"
                     /translation="MVEIMRTMAHLGIGAMVKTKELLEELTEKGKRDQGPTAKWAKDC
                     LDSFEEVTKKAEKELKPCLDKFYQAANLPTRSDLERLEKKIQELSNRLGSEDRRGK"
     gene            complement(3255..>4419)
                     /locus_tag="VNV63_01670"
     CDS             complement(3255..>4419)
                     /locus_tag="VNV63_01670"
                     /inference="COORDINATES: protein motif:HMM:NF014342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HXC61358.1"
                     /translation="ELVFDQTPFYGESGGQTGDQGLLEHPSALVEITNTIKPIPGLFV
                     HQGKVAHGEIVEAETYLAIVNRLARQGASRNHTATHVLHSVLREVLGEHVKQAGSLVA
                     PDRLRFDFSHFAALTPQELKRIEAAVNERVRSDHPVETQVMGFQEAVRSGALAFFDDK
                     YGDQVRVVKIADFSKELCGGTHCRETGEVGVFRLIQESSIAAGMRRIEALTGEMAYMS
                     IKKQEEDLQELAAMLKVQPIEVVEKTRKMLAMLREREKELEQHRNRAVAAHAEDILFE
                     VRETHGVKVLIKRQDGLDQKELRTFADSIRDRLKSDVVLLASVKEEKVSLLAMVTKDL
                     AGRFHAGEILKEVAAVIGGTGGGRPEMAQGGGKEAARLDEALIRGWEFIKTRK"
BASE COUNT         1009 a         1326 c         1017 g         1067 t
ORIGIN      
        1 gctcaaactt ctcagctgtc tctcgccgtt gaacctccag gacatcgacc atctctttca
       61 tggtcttgtg ctcgactttt tttcccttag cttcgtgcca gatcacctgg cctgtctgac
      121 gatccaccag gagcgtcgcc ccgcaacaag agcacgtaac gttcaatttc tcctcgtctg
      181 acatgatccc atctccatcc gctatcgcct aatgtcctca ttgtactaca atcggcggtt
      241 ggaaggaaac acttgcttgc aacccccttc cgaggatgtt agactacgac tttacttgta
      301 tcacccgtat gagaaaaaat aatgaaacct gtctggacaa ttagcttcgc cgttctcatt
      361 acaataatat tccttatcaa ttctgcacgg gcaatgctga tggaccggat cctcgccgtg
      421 gtaaaccgtc aacccgtcac tttaagcgat ctccatcggt acgaggcctt ccttgcgttg
      481 agcgacggta aaaagaactg ggagccccca ccctctccct ccccaaccat gttacatcag
      541 ctcattgacc agaagcttct tcttgaggag gctgagaagt ttgaggtcag tccgtccaag
      601 acgaccgatc ttctcgaggc gcaggatcgg ctggccaaac ggtttacaaa ccccgaagag
      661 tttcaaaaca cactcctgcg ctgggggctg gatcgggaga gcctacacca cgagatcgaa
      721 gatcatctca tggtagaacg ctttatcgat cagaggatca cattttttgt gatcatcccg
      781 actgccgaga ttcaaaaata ttatgaagcc catcgggagg agtatagaga gaaacggctg
      841 gaagaggctc tcgcccatat cacgaggcaa ttgaccgacc taaaggcaaa ggaaaaactg
      901 gaatcctatc ttaaaaagct gagggagaaa gcaagcatcc aaataaattc ttaatcctaa
      961 ttttctaagg aaatgatcag ttccaactcg atcggggagg agagaggcag ctccgccgca
     1021 cccaaggcca ggcgggcatg ttttccgcgt tcaccgaaca tctttaacaa cagatccgag
     1081 gctccattaa gcacggccgc ctgttgagta aagccaggtg ccgaagtcac atatccggtc
     1141 agccgtacga cccgggccac acggctcaga tctccaattt cctgccggat cacggcaagg
     1201 gcattcaaga ccgctgtccg ggcagactcg taaccctgct cgatcgtaag gtccatcccc
     1261 aatttcccct gatattgaag ttctcctaga atcaaaggaa gcataccgct caggaaaaga
     1321 agattgcccg ctcgaaccgc aggcacatag gcccctaacg gtttcggagc atccggcagg
     1381 ttcaatccaa gttctttgag aatctcctct ggtgattttg acatttaaga gttttccctc
     1441 accaaggttt tccctccctt ccgtttctga tacttctgaa cggctcgatt gtgctctcga
     1501 agagtggctg aaaagtaatg cgttccatca ttcttggata caaagtagag ataatctaca
     1561 ttgtccggat acaaggcagc atagagagat tcgcgacccg ggctggcaat cggcccgggt
     1621 ggcaggccct tattttggta ggtgttgtac ggcgatttaa ctttaagctg ttttttgtgg
     1681 agtgtcccat gaaagtcagg cagaagcgca tagatgacgg ttggatcgct ctgcaaagga
     1741 atattccgct tcaaccggtt gtggaaaacg gccgatacaa gccgccgttc atattccacc
     1801 gaagtttcct tctcaatgat cgaggccagc gtgataacct cgttctgggt catattcagc
     1861 tccgcggcac gcgtctcgag ctcgggctca aaagtatttt taaatttcgc aaccattgtc
     1921 ttgatcacct cctcggcacc gatcctttta ggaaagtagt aggtgtccgg aaacagatag
     1981 ccctcgagac cgtctgtctt gagtccgagg gaacgaataa aatctggatc atggactaga
     2041 accataaagg tatcatagtc cgccatcttc aggtcttcga cgagatgagc aatctgatcc
     2101 gctgtgtagc cctccggtac caccagctca taagtcagga ccctgccttt cttgagcgca
     2161 tccataatct caagcggcgt catccgggtc gtgaaggcat attcccccgg tttgattctc
     2221 cgctcagcaa gcgtcagctt ccctagcaga atgaaataag gcacccgggt gatcaggctt
     2281 tctcgggaca agagggtggc aacgtcgcgt agcgtggcac cagcctgtac ttcgatcacc
     2341 ttgttcactt cttccttgct ggatgggatg tacagaaacg ttaagaaatg aacaacgagg
     2401 aaaaagataa ggcttccaac tagaaagacc gggatcaaga accgactgag ggttttggag
     2461 ccgcccttct gcatcaaacc tcgccgatgg gatgagacgc agtctgcctg ccggacaggc
     2521 ggacctgatg ggcatcaaga tagttctgca ggatcagaac cgctgccatc tgatccacca
     2581 cggtctttcg cttgcttcgc ctgacatgcg cctcgatcag tgtgcgttcc gcctcccggc
     2641 tcgtcaatcg ctcatcccac atcaggactg gcaccggcat tcgtgttttt agcgactcca
     2701 caaacgtcaa taccttctgg gcctggggac cgatgtgtcc atcgagatgg cggggcaaac
     2761 caaccacaac ctcggtgact tctctactta gaatcaactc tgtcagatac aaaagatccc
     2821 gcttcaagtc tttgcgtatg agcgtttcaa gggcctgcgc ggtccagccc atctcatcac
     2881 taattgctaa accgattcgc cgatcaccca catccagggc caagatcctc atccgctcat
     2941 tttccccggc gatcttccga gcctaaccgg ttggacagct cttggatttt cttttccaga
     3001 cgctccagat ccgatcgggt cggcaggttg gccgcctggt agaatttatc caggcagggt
     3061 ttaagctctt tctcagcctt cttggtcacc tcttcgaaac tgtcaaggca gtccttggcc
     3121 cacttggcag tcggaccctg gtcccgcttc cccttctcgg tcaactcctc aaggagttct
     3181 ttcgtcttaa ccatggctcc gattccgaga tgggccatgg tcctcatgat ttctaccatc
     3241 gtcttcacct ccatctattt tcttgttttg atgaattccc atcccctgat caaggcctcg
     3301 tccagacgcg ccgcctcttt cccgcctcct tgagccattt ccggccggcc gccgccggtc
     3361 ccgccgatca cagccgctac ctcctttaaa atctccccgg catgaaaacg gccggcgaga
     3421 tcttttgtca ccatcgccag aagcgacacc ttttcctctt tgaccgatgc caggagtacc
     3481 acatctgatt ttaaccgatc ccggatggaa tctgcaaagg tccggagctc tttctgatcg
     3541 agaccatcct ggcgcttgat caggaccttc acgccatgag tttccctgac ctcgaacagt
     3601 atatcttcgg catgtgcagc caccgcccgg tttctgtgtt gctcaagctc cttctcacgc
     3661 tcccgcagca tcgccagcat cttccgtgtc ttctccacta cctcaatcgg ctggaccttg
     3721 agcatcgccg ccaattcttg gagatcctct tcctgctttt tgatagacat gtaggccatc
     3781 tcaccagtaa gcgcctcgat ccggcgcatc ccggccgcaa tgctgctctc ctgaatcagc
     3841 cggaagaccc ccacctctcc ggtctcgcgg cagtgtgtcc caccgcacag ctccttgctg
     3901 aaatccgcga tcttcaccac gcgaacctga tccccgtatt tgtcatcgaa gaaggccaaa
     3961 gccccggacc gcaccgcctc ctgaaaaccc atcacctgtg tctcgaccgg atgatcgctc
     4021 ctcacacgct catttaccgc cgcttcaatc cgctttaact cttgtggagt caacgcagcg
     4081 aagtggctga aatcgaatct tagccgatcc ggtgcgacca acgatcctgc ctgcttgaca
     4141 tgctccccca ggacctcacg cagaaccgaa tgcaaaacat gcgtcgcagt atgattgcgc
     4201 gatgccccct gtctcgctaa acgattcacg atggccagat aagtttctgc ctcgacaatc
     4261 tcgccgtgcg ccacctttcc ctgatggaca aagagtcccg gaatcggttt gatcgtgttc
     4321 gtgatctcca ccagcgcgct cggatgttcc agcagccctt gatccccggt ctggccgccg
     4381 gactctccat aaaacggcgt ctgatcaaac accagctca
//
LOCUS       DATMKH010000133         1453 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3271978, whole genome shotgun sequence.
ACCESSION   DATMKH010000133 DATMKH010000000
VERSION     DATMKH010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3271978"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="VNV63_01675"
     CDS             complement(<1..270)
                     /locus_tag="VNV63_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61359.1"
                     /translation="MKTRLAKKIILLFIVAAGVSGFSFAHAVDISLSIPTQNGNNPCA
                     SPTATTCYPGTYVNTFYQFALMIGGIIAFGAIVFGGVKYMTSAGNP"
     gene            complement(267..971)
                     /locus_tag="VNV63_01680"
     CDS             complement(267..971)
                     /locus_tag="VNV63_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61360.1"
                     /translation="MIRSRKNLAMGLGILTALFFALVAPTARAASAMPQFLITWKATG
                     SYVPTNYQGKALPTYGSQITASLEIISNGQVANLQGQTIYWYLNDTLLGGGTGAQTIT
                     FPPFGQAPNTMTLKVELPSYNGNFLIHTIAVPMVQPQAIIYAPYPNGQFSTNPVAVTA
                     VPYFFNTASPDDLSYAWSVNNQSGANTENPEEADITLPAGTAAGTTFEVSVNVQNGND
                     STVATISKTLTYQPSL"
     gene            complement(994..>1453)
                     /locus_tag="VNV63_01685"
     CDS             complement(994..>1453)
                     /locus_tag="VNV63_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61361.1"
                     /translation="GEAAEGAEMAGEIGAGAEEAGVAEAGEEVGASEETSVKEKMETE
                     EEGGAEGPREEREKSEREKEIEEELTPEEEKNPLEVESRKLNEGPDEDLRDDETDEDD
                     LPSSPKKPAASERSARNSRPKLQDIKDINNSPLDKSDDDETDTPAREEAA"
BASE COUNT          288 a          378 c          385 g          402 t
ORIGIN      
        1 aggatttccg gcagacgtca tgtacttaac gccgccgaaa acgatcgcgc cgaacgcaat
       61 gatccccccg atcatcagtg caaattgata aaaagtattt acataggtgc cggggtagca
      121 tgtggtcgca gtaggtgatg cacatggatt gttgccattt tgtgttggga tagagaggga
      181 gatatctacc gcatgtgcaa aagagaaacc tgaaacgccg gccgcgacaa taaagagcaa
      241 aataatcttt ttagctagcc tggttttcat agtgacggtt gatacgtaag ggttttggat
      301 atcgttgcga ctgtggagtc gttgccattt tggacattga ccgagacttc aaaggttgtt
      361 cctgcggccg tgcctgcagg gagcgtgata tctgcctctt ctggattttc cgtgttggct
      421 ccggattgat tgttcaccga ccacgcatag gagagatcat ccggtgatgc cgtattgaaa
      481 aaataaggga cagcagtgac cgctaccgga tttgtagaga attgtccgtt cggatagggc
      541 gcatagatga tggcttgcgg ttggaccatg ggaacggcaa tggtgtggat aaggaagttg
      601 ccattgtagc tcggtagctc cactttaagc gtcatggtgt ttggcgcctg gccgaaagga
      661 gggaacgtga tcgtctgtgc ccctgtaccg cccccgagga gcgtgtcgtt cagataccaa
      721 tagatggtct ggccctgaag attcgccact tgcccgttgg aaattatttc gagcgatgca
      781 gtaatttgcg agccgtaggt cgggagggct ttgccctgat agttggtcgg gacatagctt
      841 ccggtcgctt tccatgtgat caagaattga ggcatggccg aggcagctct cgcggtaggg
      901 gcgacgagcg caaaaaataa ggccgttagt atgcccaggc ccattgccag atttttacgc
      961 gagcggatca tattcttcct ttattgtagc aaattaggcc gcttcttccc tggctggcgt
     1021 atcggtctcg tcatcatcgc ttttatcaag cggagaattg ttaatatcct tgatatcttg
     1081 aagcttcggt ctggaatttc gcgccgatct ttcgctcgcc gctggtttct taggcgaaga
     1141 gggcaggtcg tcttcatcag tttcatcatc ccggagatcc tcatcgggcc cctcattgag
     1201 ctttctgctt tcgacctcaa gcgggttctt ttcctcctcg ggcgtgagtt cttcctctat
     1261 ctctttttct cgctccgatt tttctctttc ctcccgcggc ccttccgcac cgccctcttc
     1321 ttcggtttcc attttttcct tcaccgaagt ttcttctgag gcgcccacct cttcaccagc
     1381 ctccgcgacc ccagcttcct cggcccctgc accgatctca cccgccatct ctgcgccctc
     1441 tgccgcttcg ccg
//
LOCUS       DATMKH010000134         1032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4233849, whole genome shotgun sequence.
ACCESSION   DATMKH010000134 DATMKH010000000
VERSION     DATMKH010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4233849"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..291)
                     /locus_tag="VNV63_01690"
     CDS             complement(163..291)
                     /locus_tag="VNV63_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61362.1"
                     /translation="MIDHGAHLFQGFHFEVTLGDELVQRSQIENGTEVTGFLRNGK"
     gene            232..>1032
                     /locus_tag="VNV63_01695"
     CDS             232..>1032
                     /locus_tag="VNV63_01695"
                     /inference="COORDINATES: protein motif:HMM:NF012307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HXC61363.1"
                     /translation="MPQRHFKMETLKQVCTMINQGDYLTSIDLTDAFMHVLVHQSSRR
                     FLQFTWKGRLFQFRVLPFGMSLSPMVFTKILKPVLRWARRKGIRLTAYLDDLLIVAKD
                     RATSLRRTELVLHKLADLGFPIKRSKSSLIPSQSIQHLGFTINTTNLSLTVPTSKIRN
                     LRREASRLLRRPTSTIRALASFIGKSQSMTIAIFPARLKTRHLLLCKNMALHQTKSWT
                     SPLSLSPAAKDELMWWKEQLLSWNGQSFLPSSPQVEIYTDASDSGWGIV"
BASE COUNT          254 a          339 c          189 g          250 t
ORIGIN      
        1 tcctttcacg tccccaccgc caatgcaatc tcttcctccc gccacatctt ctgctcttcc
       61 caaccccaat ccaatttccc tatcgaaaaa gagatcctag ctatgctaag caagaaggca
      121 atcgaaaagg tccatcccaa ctcctatggc tttcgttccc ggttatttac cgttccgaag
      181 aaaaccggtg acctccgtcc cgttctcaat ctgcgaccgc tgaaccagct catcccccag
      241 cgtcacttca aaatggaaac cctgaaacag gtgtgcacca tgatcaatca aggcgattac
      301 ttaaccagca tcgacctgac agacgccttt atgcacgtcc tcgtgcacca gtcttccagg
      361 cgtttccttc aattcacctg gaaaggtcga ctgttccagt tccgcgtcct cccgttcgga
      421 atgtctctgt cccccatggt tttcaccaag atcctcaaac cagtactacg ttgggctcgt
      481 cgcaaaggga ttcgtcttac cgcttacctc gacgacttgc ttatcgtggc caaggaccgc
      541 gcaacttctc tacgtcgtac ggaacttgtc ctccacaagc tggccgactt aggatttccg
      601 atcaaacgga gcaagtcatc actgatccca tcacaatcca ttcaacatct cggtttcacg
      661 atcaacacca ccaacctctc cttaacggta ccgacatcca agatccgcaa cctcaggcgg
      721 gaagcttctc gtttgctacg ccgtccaacg agcaccatac gagccctcgc ttccttcatt
      781 ggaaaatccc aatccatgac gatagctatc tttcccgcac gcttaaaaac ccgacactta
      841 ctcctgtgca agaacatggc tcttcatcaa accaaatctt ggacatctcc actctctcta
      901 tctcccgcag ccaaggacga gttaatgtgg tggaaagaac agcttctatc ttggaacggc
      961 cagagttttc ttccctcatc accacaggta gaaatctata ccgacgcatc cgattcgggc
     1021 tggggaatag tg
//
LOCUS       DATMKH010000135         1851 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1667003, whole genome shotgun sequence.
ACCESSION   DATMKH010000135 DATMKH010000000
VERSION     DATMKH010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1667003"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(185..>1851)
                     /locus_tag="VNV63_01700"
     CDS             complement(185..>1851)
                     /locus_tag="VNV63_01700"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HXC61364.1"
                     /translation="ARNQDTIQNIRLWDYRPLLQMYQQAQEIRLYYQFYQADVDRYHL
                     PDGYHQVMLSARELSPELPAQAQTWVNQKLQFTHGYGLVMSFVSKTMGGGFPQYVLEN
                     IPPESAYGLAINQPAIYYGESMTGYRIVATGVKEFDYPKGNQNVYTSYAGTGGIPLDS
                     FWKRLLFAWTQKDVNILLTSYLQPESKIQIWRRVQERVQQIAPFLRLDKDPYAVVSEG
                     KQYWIQDAYTVSNHFPYSSPHAADFEAGLNYIRNSVKVVVNMYDGTVRFYVMDPSDPV
                     LAVYRRAFPGVFMDLNQLSPDLKAHLRYPEDLFSIQADQYTTFHMTVPQVFYNREDLW
                     VLPQENYAGKVAPMKPYYILMKLPGSDQLEYLLMTPFTPQKRNNMISWMAARCDFPDY
                     GKTLFYQLPKEKLIYGPMQIEAMIDQNTTISQQLSLWDQKGSRVIRGNLIAVPIENSF
                     LYVVPLYLTAEGTDFPQLKRVIVISGDKVAMEPTLDEAIQSVFGTAQPQISAQAPGAQ
                     PELGQARAQFDDAQKAMQQGNWADFGKSMDALKRLLNAPAPTGVRQ"
BASE COUNT          401 a          476 c          514 g          460 t
ORIGIN      
        1 ccatggacgg gaactcttgg tcactgtgat gaatcgtgac cgttctttcg ggatgtttta
       61 tcagcgtgta gtcgcatctc caatacccct gttgcagctc ttgatgcatc tccatttcaa
      121 tctcgatgcc tttgtagatg gattgaatca tgaatcgtac actccttcat tagaataagg
      181 ttcgttattg tcgaactcca gtgggtgcgg gcgcgttcag caagcgtttc agtgcgtcca
      241 tcgatttgcc gaagtctgcc caattgccct gctgcatggc cttctgtgca tcatcgaact
      301 gcgctctggc ttggcccagt tccggttgcg cgccgggcgc ctgcgcggaa atctggggtt
      361 gcgcggtccc aaaaacggat tggatagcct catcgagtgt cggctccatc gctaccttat
      421 cccccgaaat cacgattacg cgctttagtt gcgggaaatc agtgccctct gccgtgaggt
      481 acagcggcac cacatagaga aaagagttct cgattggaac cgcgatcagg tttccacgaa
      541 tgactcgcga tcccttttgg tcccacaggc tcagttgctg ggagatggtg gtgttctgat
      601 cgatcattgc ttcgatctgc atcgggccgt aaatcagctt ctctttcggg agttggtaaa
      661 agagcgtctt gccgtagtcg ggaaaatcgc accgggccgc catccaggag atcatgttgt
      721 ttcgcttctg gggcgtgaag ggtgtcatga gaagatactc gagctggtcg cttccgggaa
      781 gcttcatcag gatgtaatag ggcttcatgg gcgcaacctt tcccgcatag ttctcttggg
      841 gaagcaccca cagatcctcc cggttgtaga aaacctgcgg aaccgtcata tggaatgttg
      901 tgtactggtc agcctggatg gaaaaaagat cttccggata gcgcagatgc gctttcaaat
      961 ccggcgaaag ctgattcaga tccatgaata cgcctggaaa cgcacgacgg tagacagcca
     1021 ggaccgggtc actcggatcc atgacgtaga accggaccgt tccgtcgtac atgttcacaa
     1081 ctaccttcac ggagtttcga atataattca accctgcttc aaaatcggcg gcgtgaggac
     1141 tggagtaggg aaagtggttc gagaccgtgt atgcatcctg gatccagtat tgtttgccct
     1201 cgctcactac tgcatagggg tccttgtcga gacgcaagaa gggagcgatc tgctgcacgc
     1261 gttcctggac acgcctccag atctgaatct tgctctcagg ttgaagatag gaagtaagca
     1321 gaatgttgac atccttctgg gtccaggcga ataagagccg cttccagaag ctgtccagcg
     1381 ggatacctcc cgttccggcg taactcgtgt agacattctg attccccttg gggtagtcga
     1441 actctttcac cccagtcgcg acgatccggt atccagtcat cgactcgcca tagtagatgg
     1501 caggctggtt tatagctagc ccgtatgcgg attcgggcgg gatattttca agtacgtact
     1561 gcgggaagcc tccccccatg gtctttgaga cgaagctcat gaccaggccg taaccatgcg
     1621 tgaattgcaa cttctggttc acccaggtct gggcttgagc gggaagttcc ggggaaagct
     1681 cgcgagccga aagcatcacc tggtgataac catcgggcag atggtagcga tcgacatcgg
     1741 cctggtaaaa ctggtaatag agccgtattt cctgggcctg ctggtacatc tgaagcaaag
     1801 gacggtagtc ccagagccgg atgttctgga tggtgtcttg gtttctcgca a
//
LOCUS       DATMKH010000136         1532 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2632366, whole genome shotgun sequence.
ACCESSION   DATMKH010000136 DATMKH010000000
VERSION     DATMKH010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2632366"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..833
                     /gene="trpS"
                     /locus_tag="VNV63_01705"
     CDS             <1..833
                     /gene="trpS"
                     /locus_tag="VNV63_01705"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020877928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HXC61365.1"
                     /translation="ADTSRIREYIQEMLVDWLAVGMEPEKCTLFQQSQVPDHAVLHLL
                     LSMITPIPWLERNPTYKEQQQELGDRDLATYGFLGYPVLQSADILIYKAQFVPVGVDQ
                     LPHLELTREIARRFNSLYRPVLVEPEALLTETPKILGVDRRKMSKSYGNAIYLSDSPD
                     VTEKTVQKMITDPLKIKKKDPGRPEICNVFTYHTIFSSKDEVQQVNRDCRSGALGCVD
                     CKTWMAKNLNTYLEPIRKKRAELEKEPEKLDKILSEGSTRARKVAQKTLDEVKEAMKI
                     "
     gene            942..>1532
                     /locus_tag="VNV63_01710"
     CDS             942..>1532
                     /locus_tag="VNV63_01710"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HXC61366.1"
                     /translation="MDESQTSYAVKLPAFEGPLDLLLHLIKTNEINIYDIPIAMITQQ
                     YLDYLKLMKNLNLGIAGEFIVMAATLIYIKSKTLLPLEESPLEEQEEDPRAELVRRLL
                     EYKQFKEAASTLAEREQEWRDVFRREEKGDNGHEVTLTDLNLFDLLDALQKVLEQVPS
                     KRSVDILIDGLTVKDRMSIVLSGLEAADGIDFLSLFE"
BASE COUNT          402 a          347 c          402 g          381 t
ORIGIN      
        1 atgccgatac aagccgtatc cgggaatata ttcaggagat gcttgtggac tggcttgcgg
       61 tcggcatgga accggaaaaa tgcacactgt ttcagcagtc ccaagttccg gatcatgcgg
      121 tgctccatct cctgctttcg atgattacgc cgatcccgtg gctggagcga aatccgacct
      181 acaaggagca gcagcaggaa cttggcgatc gagaccttgc cacatacggt ttcttaggct
      241 atcccgtttt gcagtcagcg gacatattga tctacaaagc ccaatttgtt ccggtgggag
      301 tggatcagct cccgcatctg gaacttacgc gggagattgc ccggcggttt aactcattgt
      361 atcggccggt tcttgtcgaa cctgaagcgc ttttgacaga gactcccaag attttgggtg
      421 ttgaccgccg gaagatgagc aagagctatg ggaatgccat ctacctttcg gattctccgg
      481 atgttacgga gaagacggta cagaagatga tcacggaccc tttgaagatc aagaagaagg
      541 atcccgggag accggagatc tgcaacgtat ttacttatca cacgatcttc tcgagtaagg
      601 atgaagttca gcaggttaat cgggactgcc ggagtggcgc gttgggatgt gtggactgca
      661 agacctggat ggccaagaat ctaaatacct acctggagcc gattcgcaaa aagagggcgg
      721 aactcgaaaa agaacccgaa aaactggata agattctttc ggaaggctcc actcgcgccc
      781 gcaaggttgc acagaagaca ttggatgaag ttaaagaagc gatgaagatt taacttgtat
      841 cttaatccac aatatcttgt cgcctttttg tccttcttat accatatctt gttttttaac
      901 ttgactaacc ttacggaaga taatatagtt tcagcatcgt tatggatgaa tcacagacat
      961 cctatgccgt aaagcttccg gcctttgaag ggccgctgga tcttctgctg cacctcatca
     1021 agacaaacga gatcaacatc tacgatattc ccattgccat gatcacacag cagtatctcg
     1081 actatctcaa gctgatgaag aatctcaacc ttgggattgc gggtgaattt attgtaatgg
     1141 ccgccaccct gatctatatc aaatctaaaa cacttttacc gctggaggaa tcgcctctcg
     1201 aagagcagga agaagacccg cgggcggagc tggtccgccg gttgttggag tacaagcagt
     1261 ttaaggaggc cgcttcaacg ctagctgagc gggagcagga atggcgtgat gtgtttcgac
     1321 gagaggaaaa aggggacaat gggcatgagg tgacattaac cgatctcaat ctgtttgacc
     1381 tcttggatgc ccttcagaag gttctggagc aggtgccgtc caaacgatcg gttgatatcc
     1441 tgattgatgg attgaccgtt aaagacagga tgtcgattgt cttgagcgga ctggaagcgg
     1501 cagatggtat agacttctta tctctttttg aa
//
LOCUS       DATMKH010000137         1830 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4234418, whole genome shotgun sequence.
ACCESSION   DATMKH010000137 DATMKH010000000
VERSION     DATMKH010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4234418"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..852)
                     /locus_tag="VNV63_01715"
     CDS             complement(19..852)
                     /locus_tag="VNV63_01715"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HXC61367.1"
                     /translation="MQAVRYHFKEKFWNHVFPWLPLPVTLPWGGWWLAFNDGFGTGVF
                     ARITGGSLDNFLLRFLKPKMTVFDVGAHQGYYTLLISKLVGGQGSVIAFEPSSREFKR
                     LFRHLRINRCKNVRANQLALSDVNGTADFFLIKGLWTTRNSLHPPANHEIQRVSVPIT
                     TLDAYLKRESSDDVDLIKLDVEGAELSVLEGSAELLSQTRRPTILCELNDEVISWGKW
                     GHCGESIVEFLERKNFRWFSITTEGRLEPLVRSGVYCGDYAAVPFERLEALGPLIVDR
                     I"
     gene            complement(911..>1830)
                     /locus_tag="VNV63_01720"
     CDS             complement(911..>1830)
                     /locus_tag="VNV63_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014797086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXC61368.1"
                     /translation="TLLEVGTGFHPELTGRENVFLSGAILGMGKAEIRRKFDEIVDFA
                     EVEKFIDTPLKHYSSGMQMRLAFAVAAHLEPEILLVDEVLSVGDIEFQKKCLGKMGDV
                     ARRGRTILFVSHQMNQMRRLCDSCCWVDQGGVRMWGKTVEVVNRYEEAGKPKLRQEET
                     RESGSAHLAVCFVSWRIVGNSEMDVSVLKSPGPTTLCFCLLINRPIKKGIFGLTLRDS
                     RGQINWAVAQFNLNLFPGPCEFTVNVPYFPLIPGLYYWDISVFDGHAWINEGQCAPAF
                     AVETHDFSHPYDEFRGFLNLPYEFRMHNS"
BASE COUNT          440 a          523 c          447 g          420 t
ORIGIN      
        1 gcggcgtgtc ggcatgttct atatccggtc cacgatcaat ggaccaaggg cttcgagccg
       61 ttcgaacgga accgcggcat agtccccaca atagactcca cttctcacca aaggctctaa
      121 ccttccttcc gttgtgatcg aaaaccatcg aaagttcttc cgttcgagga actctacgat
      181 cgattcgccg caatggcccc actttcccca cgaaattact tcatcgttca gctcgcagag
      241 gattgtcgga cgcctggtct gagaaagcag ttccgcagat ccttcgagta ccgacaattc
      301 cgcaccttcc acgtctagct taatcaggtc gacgtcgtca ctgctttccc gcttcaagta
      361 ggcgtctaac gtcgtgatag gaacggacac gcgttgaatc tcgtgattcg cgggcggatg
      421 taggctgttc ctggttgtcc aaaggccctt tatgagaaag aaatctgccg taccgttgac
      481 atcagagagg gctagctggt tcgcgcggac attcttgcat cgatttatcc tgagatgtcg
      541 aaataacctc ttgaattccc gggacgacgg ctcaaaagcg atgacagagc cctgaccgcc
      601 caccaatttg ctgatcagca gggtgtagta gccttggtgt gctcccacat caaatacggt
      661 catcttgggc ttcaggaagc gaagaaggaa attgtccaaa gatccgcccg tgattcgggc
      721 gaacactccg gtaccgaaac cgtcattgaa ggccagccac catccacccc aaggcagagt
      781 aaccgggaga ggcagccacg ggaaaacatg gttccagaac ttctctttga aatggtagcg
      841 aaccgcctgc atcttcgatg tagttggctt ccgccaaaat cgaagagtcc gagaaagcag
      901 gtctccggtc tcaggaattg tgcatccgga attcataagg tagattcaag aagcccctaa
      961 actcgtcgta gggatgagag aagtcatgtg tttcgacggc aaaggccggc gcgcactggc
     1021 cctcgtttat ccaggcatgg ccgtcaaaaa cagatatatc ccaatagtaa aggccaggaa
     1081 tcaagggaaa atatggtacg ttaaccgtga actcgcaagg gccgggaaac aggttgaggt
     1141 taaactgcgc cactgcccaa ttgatctggc ctcggctgtc ccgcaaggtg agaccgaaaa
     1201 tccccttctt gatgggccta ttgatcaaaa gacagaaaca aagggtcgtc ggacctgggg
     1261 attttagaac acttacatcc atctcgctat tcccaacgat acgccagctc acgaaacaga
     1321 cagccagatg ggccgatccc gattcccttg tctcttcctg tcttagcttt ggtttgccgg
     1381 cttcctcgta gcgattgacg acctctactg ttttacccca catccgcaca ccaccttggt
     1441 ccacccagca gcaagaatca cacaggcgcc tcatctggtt catctgatgg ctgacgaata
     1501 gaattgttct tcccctcctg gccacatccc ccatctttcc cagacatttt ttctggaact
     1561 ctatgtctcc gaccgatagc acttcatcca ccagcaggat ttccggttcc aggtgcgccg
     1621 ctacggcaaa cgctaggcgc atctgcattc cgctgctgta atgttttagc ggagtgtcga
     1681 tgaacttttc cacttcggcg aagtcgacga tctcgtcgaa cttgcggcga atctcggcct
     1741 tgcccatgcc caggatggcg ccactcagaa agacattttc gcgcccggta agctcggggt
     1801 gaaaaccggt tcccacttcg agcaaagttc
//
LOCUS       DATMKH010000138         2611 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1668347, whole genome shotgun sequence.
ACCESSION   DATMKH010000138 DATMKH010000000
VERSION     DATMKH010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1668347"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="VNV63_01725"
     CDS             <1..416
                     /locus_tag="VNV63_01725"
                     /inference="COORDINATES: protein motif:HMM:NF016959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HXC61369.1"
                     /translation="ILKEKLADLEIQVLEFKKKENTLNSTLMKAQDLVEEMKGHAQRE
                     GELTIKEAELKAEETAKAARERLAELRREILDLQKQKLLFIEKTRSLIKIVHRVLELE
                     EREGEREESGRESRLDIEREENIRLLKPKEKSPGA"
     gene            576..1043
                     /locus_tag="VNV63_01730"
     CDS             576..1043
                     /locus_tag="VNV63_01730"
                     /inference="COORDINATES: protein motif:HMM:NF027482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4864 domain-containing protein"
                     /protein_id="HXC61370.1"
                     /translation="MSKPQHICRIFTCLVFAPLSVFLVGLSNAQGTAQTKPQEEKIRS
                     VIQQQLNAFNRDDYDAAYSLASRHIQTEFSRPEFEQMVKNGYPQIARSSRAMFEKIAF
                     LETESRAVATVTITGLDRITITADYRIVWEDGKWRIDGVMIIDQQMPISYEPD"
     gene            1030..2451
                     /gene="mpl"
                     /locus_tag="VNV63_01735"
     CDS             1030..2451
                     /gene="mpl"
                     /locus_tag="VNV63_01735"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HXC61371.1"
                     /translation="MNRTEFKLQHIHMIAIGGTGMSALAGLLKAAGYYVAGSDGDLYP
                     PVSSLLARLGITRSTGYRPENIAPGTDLVVVGNAVSKSNPEVLAAQERGLRMLSFPQT
                     LSEFFLKDKQPIVVAGTHGKTTTTSLMAWVLESAGLQPGTMIGGWAKSFNGNYQLGKG
                     RYFVVEGDEYDTAFFDKGPKFLHYRPAMAILTSIEFDHGDIYLNLDAIKQAFSAFVRL
                     LPKDGRLIKVYDDARIQEVTAGLDCPQETYGLDPAADWWADHLVPQPESLTFVVHHQR
                     KVIGQVNSPLLGKHNILNTLAVIAMATHLGLSWDRIKSGIESFQGIKRRQEIIGEAHD
                     ILVMDDFAHHPTAIHETLSAVRLHYPGRRIWAIFEPRSATSRQKVFQAEFAQSFGPAD
                     WIVIADIFAKEKIPVENRLDLAKLVRDLKLKGQAAEFIPTADEIVERVGPQLQSGDLV
                     CILSSGGFGGLHQKLLKFLEEHA"
BASE COUNT          656 a          654 c          725 g          576 t
ORIGIN      
        1 atatcttgaa agaaaagctg gccgatttgg agatacaggt cctggaattc aaaaaaaagg
       61 agaacactct caacagcacc ttgatgaagg cccaggacct tgtggaggag atgaagggcc
      121 atgcccaacg agaaggtgaa ctgaccatta aagaggcaga gctcaaagcg gaagaaaccg
      181 cgaaggcggc ccgagaacga ctggctgaac tgaggcggga aatactggat ctgcaaaaac
      241 aaaaactcct gtttatagaa aagacgcgtt ccctgattaa gatcgtacac cgtgtcctgg
      301 aactggaaga acgggagggg gagcgggagg agtccggcag ggaaagccgt ctggacatcg
      361 aacgggagga gaacatccga ctgctcaagc caaaagagaa atctccgggt gcatgacatg
      421 agatggccat ggaattagaa tctacgtgag acatgcacta ctaataagga gaagcggatg
      481 agttattgcg tcttcatcag ccggcgagct aaagaaaatc tgtaaaatca gggcgttagc
      541 ttttccccgc atcttaaatt ataataaata ctgcaatgtc caaacctcag cacatctgtc
      601 ggatctttac gtgcctcgtt tttgcgccgc tctcggtctt tcttgtaggc ctgtcgaatg
      661 cgcaggggac tgcgcagact aagccgcagg aagagaagat ccgctccgtg attcagcagc
      721 agctcaacgc atttaatcgg gatgattatg atgccgcgta ttccttggct tcccgccata
      781 ttcaaaccga attttcaagg ccggagtttg agcaaatggt gaagaacggg tatcctcaga
      841 ttgcgaggtc aagccgagcc atgttcgaga agattgcctt tttggaaaca gagagtcgtg
      901 ctgtggccac cgtgacgatt acaggattgg accggatcac cattaccgca gactatcgta
      961 tagtatggga agatggaaaa tggaggattg acggtgtcat gattatcgat cagcaaatgc
     1021 cgatttccta tgaaccggac tgagttcaaa ctccaacaca ttcatatgat cgcgatcggc
     1081 ggtaccggca tgagcgctct tgccggtctt ctgaaggcgg cgggctatta tgtagcgggt
     1141 tcggatggag atctgtatcc gccggtcagc agccttcttg cccgtctcgg gatcacacgg
     1201 tcgaccggat accggccgga gaacattgcg cccggcaccg atctggtcgt ggtggggaat
     1261 gcggtatcca agagcaatcc ggaggttctg gcggcccagg agcgtggtct tcgtatgctc
     1321 tcatttccgc aaaccttatc ggaatttttt ttaaaagata aacagccgat cgttgtggcc
     1381 gggacccatg gaaaaaccac gaccacctcg ctcatggcct gggtcttaga atccgccggt
     1441 ctccagcccg ggacgatgat cggcggctgg gccaagagtt ttaacggaaa ctatcaactc
     1501 gggaagggac ggtatttcgt cgtcgagggg gacgaatacg acaccgcctt cttcgataaa
     1561 ggccctaagt ttcttcatta tcgcccagcg atggcgatac tgaccagcat cgagttcgat
     1621 cacggcgata tctatctcaa tctggatgcg atcaagcagg ccttttctgc ctttgtccgg
     1681 cttctcccca aagacggccg gctgatcaag gtatatgacg acgcccgcat ccaagaggtc
     1741 acggccggac ttgactgtcc tcaggagacc tatggcctgg atcccgctgc cgactggtgg
     1801 gcggatcatc ttgtgccaca gcctgaatca cttacttttg tcgtccacca tcagagaaaa
     1861 gtgatcggac aggtcaacag cccgcttctc ggcaaacaca atatcttgaa cacactggcc
     1921 gtgatcgcaa tggcgacaca tctgggactt tcctgggatc ggatcaagtc cgggatcgag
     1981 agcttccaag ggattaagcg gcggcaagag atcatcggtg aggcacacga tattctcgtc
     2041 atggatgatt ttgcgcacca ccccaccgcg atccatgaaa ccctctctgc ggtccgtctg
     2101 cattatccgg gtcgccggat ctgggcgata tttgagccgc gatccgccac aagccgccag
     2161 aaggtttttc aggcagagtt cgcacaatcc ttcggcccgg ccgactggat cgtcattgca
     2221 gacatctttg ccaaggagaa aattccggtg gagaatcggc tggaccttgc caaacttgtt
     2281 cgtgacctaa agctaaaagg acaagcggcc gagttcattc cgacggcgga tgaaattgtg
     2341 gaacgggtcg gcccccaact ccagtccggc gatctggtct gcatactgtc cagcggcgga
     2401 ttcgggggtc tccaccagaa gctccttaag ttcctggagg agcatgcttg agcaaatttc
     2461 tcagagaatg aatcaggcgg tagaggaaaa tgtttttccg ggcgcggttg cgctggttgc
     2521 ttaccaaggg aagattgtct ttcatgaagc gtttggttca tcaagaatca ttcctcagaa
     2581 agtcccgatg actcagggga cgctgttcga t
//
LOCUS       DATMKH010000139         1281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4547050, whole genome shotgun sequence.
ACCESSION   DATMKH010000139 DATMKH010000000
VERSION     DATMKH010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4547050"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1016)
                     /locus_tag="VNV63_01740"
     CDS             complement(<1..1016)
                     /locus_tag="VNV63_01740"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HXC61372.1"
                     /translation="MKTVFRKKRLDEILIEQGLLDQKTLNEVLQRVNHDHQRLGAALL
                     EEGSLTEENLAQALAAQHQRSYVPLTDFRLDAQFFKMIPVELMYRYPFIPLKDEKGVL
                     TIVVADPGNLLAMDELEVTLKRDLAIVVSTKTAILEALKRSEGSSQVLKKIEADFHPE
                     IIREDEKGDEVLSVEKLSKDLSPVIRLVDSMILNAMQKRASDIHIEASDKEVAVKYRI
                     DGVLYRAMEPLDTKFHAPLISRIKVMSELDIAEKRVPQDGRFKMRLEGKKVDFRVSIL
                     PSIFGEDVVIRILDKEYITASVSELRLDRLGFNQEDLKKFRKSIVEPHGMALVTGPTG
                     SGKTT"
     gene            complement(1056..>1281)
                     /locus_tag="VNV63_01745"
     CDS             complement(1056..>1281)
                     /locus_tag="VNV63_01745"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HXC61373.1"
                     /translation="PRLTVEMVGVGEATGALEQLLTNAAEFHEEELDILLARVTTWVE
                     PLLLLFMGTMVATIVIIMYLPVFNLAGTIR"
BASE COUNT          279 a          399 c          300 g          303 t
ORIGIN      
        1 gtggtctttc cgctgccggt cggtccggtg accagggcca tcccgtgcgg ttcaacgatg
       61 gacttcctaa atttcttgag atcttcctgg ttaaaaccca acctgtccag cctcaattcc
      121 gatacactgg cggtgatgta ctctttgtcc aggatccgga tcaccacatc ctcaccaaag
      181 atgctgggca ggatcgagac gcggaaatcc accttcttcc cctccaaccg catcttgaac
      241 cgtccatcct gaggcacgcg tttctcggcg atatccagct cagacatcac cttgatcctg
      301 gaaatcagcg gggcatggaa cttggtgtcc aacggttcca tggcccggta caacacgcca
      361 tcaatccggt atttgaccgc cacctctttg tcgctcgcct cgatatggat atcactggcc
      421 cgtttctgca tcgcgttcag gatcatgctg tccaccaatc ggatgaccgg gctgaggtcc
      481 ttggagagct tttcgaccga cagcacctca tcccctttct catcctcccg gatgatctca
      541 ggatggaaat cagcctcgat tttctttaag acctgtgagg atccctcgct gcgtttgagc
      601 gcctcgagga tcgcagtctt ggtgctcact acaatcgcca gatcccgctt aagcgtcacc
      661 tccaactcgt ccatcgccag caggttgcct ggatccgcca ccacgatggt caaaaccccc
      721 ttctcgtctt tcaaagggat aaacgggtat cggtacatca actccaccgg aatcatttta
      781 aagaactggg cgtccagacg gaaatcggtc aacgggacat acgaccgttg atgctgtgcc
      841 gcaagggcct gagctaaatt ctcctcggta aggctgcctt cctccaggag agccgccccc
      901 aagcgctgat ggtcatgatt taccctctga agtacttcgt tcagagtctt ctgatccagg
      961 aggccctgct cgatgagtat ctcatcaagt cgcttttttc taaaaacggt cttcattcgt
     1021 aagtataccc tatcgtcagg ttctatcgcc gcaccctacc ggatcgtacc ggccagattg
     1081 aagaccggca gatacatgat gatcacgatc gtggctacca tggttcccat aaaaagaaga
     1141 agcaaaggct ccacccaggt ggtgacccgg gccaggagaa tatccagttc ttcctcatgg
     1201 aactcggccg catttgtcag aagctgctcc aatgccccag tggcctcccc gacgcccacc
     1261 atctccacgg tcaaacgggg c
//
LOCUS       DATMKH010000140         1145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3275268, whole genome shotgun sequence.
ACCESSION   DATMKH010000140 DATMKH010000000
VERSION     DATMKH010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3275268"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            224..577
                     /locus_tag="VNV63_01750"
     CDS             224..577
                     /locus_tag="VNV63_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61374.1"
                     /translation="MSITPKAIQDLLDELEASKQTRLRAWSALQRLRIVLSEMGSVAI
                     RPPAQKTFDAEGEALEHALRKSFRLRNDAIKSLCSSVRRFRDATIKEECKGDYPHAHR
                     ALWKALHRAENLIQN"
     gene            complement(650..>1145)
                     /locus_tag="VNV63_01755"
     CDS             complement(650..>1145)
                     /locus_tag="VNV63_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HXC61375.1"
                     /translation="ELGLEGLIGKRAGSRYEAGKRTGAWIKLKLHLEQEFVIGGYTNP
                     EGGRKYFGSLLVGFYEGKNLKFAGKVGTGFSDQLLRSLYSDLEKIRIEQCPFFNVPAV
                     GRSRWDQGLTAAEMRRCHWVKPIMVCQVKFTEWTRDDRLRQPVFLGLREDKKASEVLR
                     EKAT"
BASE COUNT          275 a          296 c          311 g          263 t
ORIGIN      
        1 atactgtcta tggccgttta agccgcggac ggtactcttg caccggagga cgcccgtggg
       61 ttagtgtgag tctggcgtca gccaggtagc cacaggagtg gttttaggcg aacctgggtt
      121 cgcctaggag aatgaggaag gagagacagc cttggggttc gacggggaga aagtcctttc
      181 tcccgcgcaa tgatccgtag acgggagttc tttcgtcaaa gaaatgtcga tcacaccgaa
      241 ggcaattcaa gacttgctcg atgagctgga agcatcaaag cagacccgtt tgcgtgcttg
      301 gagcgcgctg caaagactcc gcatagtact ctcggagatg ggaagcgttg cgattcgtcc
      361 gcctgctcag aagacattcg atgcagaggg agaagcgttg gaacacgctt tgaggaaatc
      421 cttccgtctc cggaacgatg caatcaaaag tctgtgttcc tcggtgcgcc gctttcgaga
      481 tgctaccatc aaggaggaat gcaaaggaga ttatccgcac gctcatcggg cgttgtggaa
      541 agctctacat cgggcggaga atctgattca gaattgaacc atgtgaagcg aacggtgcca
      601 gcaaaaagtg aaaacaatcc ggaacgattt cgttagccag gtttctaggt taggttgcct
      661 tctcccgcag gacttcgctt gccttcttgt cttctctgag tccgagaaag accggctgac
      721 gaaggcggtc atcgcgcgtc cactcagtga atttgacctg gcaaaccatt atcggcttaa
      781 cccaatggca acgtcgcatc tcagcggcgg ttaatccttg atcccaacga ctccggccaa
      841 ccgctgggac gttgaaaaat ggacattgtt caatccggat cttctccaaa tcagaataca
      901 gacttcgaag aagttgatcg ctaaagccgg ttccgacctt tccggcgaat ttcaggttct
      961 tgccttcgta aaaaccgaca agcaacgagc cgaaatattt ccggccgcct tccggattgg
     1021 tatatccgcc gatcacgaat tcttgttcaa gatgcagctt gagcttgatc caggcgccgg
     1081 tccgtttccc ggcttcgtac cgggaaccgg cgcgtttacc aatcaaacct tcgagcccaa
     1141 gttct
//
LOCUS       DATMKH010000141         1642 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2955180, whole genome shotgun sequence.
ACCESSION   DATMKH010000141 DATMKH010000000
VERSION     DATMKH010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2955180"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /locus_tag="VNV63_01760"
     CDS             complement(<1..761)
                     /locus_tag="VNV63_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXC61376.1"
                     /translation="MSIPIGEIFGFLGANGAGKTTTIRMLCGLTKPSAGSGTIGGLNI
                     WKDRFRIRSRFGYVPQKFSLYSDLTVGENLRFFAGAYRVPAGKIRERTEKVLRDMDLE
                     TKELERAGSLSGGYKQLLAIACALIHEPTLLFLDEPTAGLDPTHRQAIWDLIYELSQS
                     GTTIFVTTHYMDEAERCTDVGFVDGGRLIAKGSPRDLKENLKGHLLEVQVEPAMAAMF
                     EFRKLPGVYGADLRSGNVRLQVEDPDALLHSWQIH"
     gene            complement(907..>1642)
                     /locus_tag="VNV63_01765"
     CDS             complement(907..>1642)
                     /locus_tag="VNV63_01765"
                     /inference="COORDINATES: protein motif:HMM:NF039394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6268 family outer membrane beta-barrel
                     protein"
                     /protein_id="HXC61377.1"
                     /translation="GNITEISSSASFVLSTQVRDTVLLRLGVEWQRYSFEPEARAPVP
                     DSIQGLDLAIGADLQISPALLLRIGAHPGIYSDFRDVSWQDFNVPFEVVGTYFVSSDL
                     IFIGGVYVDLNSDFPVYPVLGIHWKVSDKWVIEGIAPRPQLQYLLSDNTTLFAGADLR
                     EASFRMNGQFGQERGIPKLNDAMLNYSEIRAGAGITLKLTKSVNLDFEGGCTPYRRFE
                     YPRADGYKVKSEDLVPYLRIGLSAKF"
BASE COUNT          399 a          465 c          405 g          373 t
ORIGIN      
        1 caatgaattt gccatgaatg aagcaaggca tccgggtctt cgacttggag acgtacattc
       61 ccgctcctga gatcggctcc gtacactccg ggcaatttgc ggaactcaaa catcgccgcc
      121 atggccggtt cgacctgcac ctccaggaga tgtcctttga gattttcttt gagatcgcgc
      181 ggggatccct tggcgatcag acgaccgcca tccacgaatc cgacgtcggt gcaacgttct
      241 gcttcgtcca tgtaatgcgt cgtaacgaag atcgtagttc cgctctggct caattcgtaa
      301 atcaaatccc agatcgcctg gcgatgcgtc ggatccagcc ccgcagtcgg ttcgtccagg
      361 aaaagcaggg tcggttcatg gatcagcgcg caagcgatcg caagaagctg tttgtaacca
      421 ccagacaagc ttccggctct ttctaattcc ttcgtctcca ggtccatatc acggagaact
      481 ttctccgtcc gttctcttat ttttccggcg ggaacccggt aagcgccggc gaaaaaacgg
      541 aggttctcac ccacggtaag atccgagtag aggctgaatt tctgcggcac atagccgaag
      601 cgcgatcgga ttctaaatcg atctttccag atgtttaaac caccgatcgt cccacttccg
      661 gcgctcggtt tggtcaaacc acaaagcatc cggatggttg ttgttttgcc ggcgccgttc
      721 gcccccagaa aaccgaatat ttcgccgatt gggatcgaca tcgaaatcct gtttacggct
      781 ttttttttgc caaacctctt cgagaggttc ttcaggacga tgagcgattc aggcatgaaa
      841 atgggataaa ggaccccgtc tcctcgaggt cctctgaaga tggatacctc agaattccgg
      901 gcgaggtcaa aactttgctg aaagtccgat ccgaagatag ggcactaaat cctcagactt
      961 cactttgtat ccgtccgctc ggggatactc gaaacgccgg taaggagtgc aacctccttc
     1021 aaaatcgagg ttgacgctct tcgtgagttt caaggttata ccggctcccg cacgaatttc
     1081 agaataattc aacatcgcat cattgagctt tgggatgcct cgctcctgac caaactggcc
     1141 gttcatccgg aacgaagctt ctcgcaaatc ggcgcccgca aacaacgtag tgttatctga
     1201 caagagatac tgaagttgcg gccgtggagc gattccctca atcacccact tgtcggaaac
     1261 cttccaatgg attcccagga caggatagac cggaaaatcg ctgttcaaat cgacgtaaac
     1321 gcctccgatg aaaatcagat ccgaactcac aaagtaagtg cccactactt cgaaaggcac
     1381 gttgaagtcc tgccacgaga cgtcccgaaa atcgctgtag atcccggggt gcgctccaat
     1441 ccggaggagc agcgccgggg aaatttgcaa atccgctccg attgccaggt ccagaccctg
     1501 gatggaatcc ggaaccgggg ctctcgcctc cggttcaaaa gagtaacgct gccactcaac
     1561 cccaaggcgc agcaacaccg tatcccggac ctgggtggac aatacgaaac tggctgaact
     1621 actgatttct gtaatgttac cg
//
LOCUS       DATMKH010000142         4603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4548100, whole genome shotgun sequence.
ACCESSION   DATMKH010000142 DATMKH010000000
VERSION     DATMKH010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4603
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4548100"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..382)
                     /locus_tag="VNV63_01770"
     CDS             complement(131..382)
                     /locus_tag="VNV63_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61378.1"
                     /translation="MAFAMEPDSIKTDGKKGEMMQGQQMTADEMVKKGDEMIAQGKEM
                     KKQGMMMKKKEMKAGSMKQDKMMNENGMAGDKMNGKGTE"
     gene            complement(605..1102)
                     /gene="msrB"
                     /locus_tag="VNV63_01775"
     CDS             complement(605..1102)
                     /gene="msrB"
                     /locus_tag="VNV63_01775"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007066965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HXC61379.1"
                     /translation="MDRRTFFKSAMAVGFGIFAGSFEPLLVRAGELDEKFEITKTDDD
                     WRSLLTPEQYNILRKEGTEPPFKNAYYKNKAHGVYLCAGCDLPLFSSDAKYDSHTGWP
                     SFWRPINDSAVQTRTDYKLIYPRTEVHCRRCGGHLGHIFDDRPPPTRLRYCINSAALK
                     FVEGQ"
     gene            complement(1141..2889)
                     /locus_tag="VNV63_01780"
     CDS             complement(1141..2889)
                     /locus_tag="VNV63_01780"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein DipZ"
                     /protein_id="HXC61380.1"
                     /translation="MNWALELFAILAGLLTVVSPCILPVLPPLLGASVATPVRHRPLW
                     IVLGLASGFTFFGTTFALFGSFLGLSSSFLRQAALVVLFFFGLSLIRPRLWEGMGNRI
                     SSLAQRITGTGKLITEQGRLNAFLIGVSLGLIWAPCAGPILGIIITLATVQRSFTQTL
                     VLMGGYSLGAAVPMLLIGYGGRRVTGRIQRFRAWGPISHKVLGFLTLVTVIGLYFNVD
                     ALVLSHLPGQLFIANWIEEKLVEKKDSVSPETDSKSGIALASTEAVPLPVLGTMPEFS
                     QITAWINSPAMSSSGLREKVVLVDFWTYSCINCIRTLPYVTQWYEKYKDQGLVIVGVH
                     TPEFSFEKEVSNIKQAVIRHGVHYPVAVDNDYGAWNAYNNRYWPAHYLIDARGRIRQE
                     HFGEGNYEETEQAIQALLAEAKLLHKPVNLEAPKGSVDFSKIHSFETYIGYGRAQNFS
                     SPQCLQRDAVLTYSAPTALGLNQWTLTGLWKITEEAAVLQASHGAIRFRFEAPKLNLV
                     MAEEEKGSTAKVLLDGKPIPLDSRGDDVGPDGKVAISGSRLYNLVTLPPGDEADHLFE
                     LIFEKPKVRLFAFTFG"
     gene            complement(3030..4097)
                     /locus_tag="VNV63_01785"
     CDS             complement(3030..4097)
                     /locus_tag="VNV63_01785"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXC61381.1"
                     /translation="MRTPKISLIWKLVAVNLLGVGIVIFLAWQVIDHFAADYFMDLMK
                     QYNIDPKVLHGMFLHTTHQFLLLSMFLGLGLVLLVSFLMTKQLMRALTQMNKITQKLG
                     RGDYSERVRVTTRDEVGELGQAFNQMVDSLGKIERMRKDLVANVAHELRTPLNNIRGQ
                     LEAIQDQLIEPSRETVDSLHEEVLRLVRLVEALHRLSQIDAETQVVTKEQFDLQALVL
                     QLLQKEQARFDRQRIRLKINGAPVAVSADADQMIQVVQNLIQNVLQYTPDGGEAGVDI
                     GIDGGRVEVRFSNSGEGIREEDLPHIFERFYRGEKSRSRESGGAGIGLAIVKQIIEVQ
                     GGTVGAHSRPGRTEIWFTLPL"
     gene            complement(4099..>4603)
                     /locus_tag="VNV63_01790"
     CDS             complement(4099..>4603)
                     /locus_tag="VNV63_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020678378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXC61382.1"
                     /translation="EVCKKIRQESDVPIMMVTAKVEEVDKLVGLSIGADDYVVKPFSP
                     REVVARVKVILRRFRRKETGPTKPILFLGLVLDPEKHKVSLHGNPLRLTHLEFKLLMT
                     FMAAPGRVFSREALLDIIYTRSEINVLDRTIDVHIKNLRQKVETNPARPRYIKTVRGI
                     GYQFAEK"
BASE COUNT         1102 a         1221 c         1100 g         1180 t
ORIGIN      
        1 ttgtcttccg ggccttttgc aaaaaccgcc gtcgacaaga tcataaacat tgctatgctt
       61 aaaactgtgt acataatttt gcttttcatt tcaatcctcc ttgacatgtt ggttaaattg
      121 ccgtttatcg ctattccgtt cccttcccat tcatcttgtc tcccgccatt ccgttctcat
      181 tcatcatctt gtcctgtttc atcgatcctg ccttcatctc ctttttcttc atcatcattc
      241 cttgcttctt catttctttt ccttgagcga tcatctcgtc acccttcttg accatctcat
      301 ctgcagtcat ctgttggcct tgcatcattt ctcctttctt accgtccgtc tttatgctgt
      361 ccggctccat cgcgaatgcc aaaaactcaa gaaaagcatt ccaaccaggg ccagcatcca
      421 aaccgaagcg tttctttctg ttgttgtctt catggcatcc tcctctttag gttagattgt
      481 tttgaaaaat gaatctttat ttacgtaagc gatcttacag aagggatatg aagcgaaaat
      541 gaaggggata tgaagattcg atgaagtccg gtgttccgga tgtttctatc taatgtatgc
      601 atctctattg gccttcgaca aacttcaatg ccgccgaatt aatgcaatac cgtaatctgg
      661 ttggcggcgg cctgtcatca aaaatatgtc ccaggtggcc gccacatctt ctgcaatgga
      721 cctcggtccg aggataaatt aatttataat cggttcttgt ttggacggcc gaatcgttga
      781 tcggtctcca aaaactcggc caaccggtgt ggctgtcata cttcgcatcg gatgaaaaca
      841 gcggcagatc acaccccgcg cacaggtaaa cgccatgggc tttatttttg taatacgcgt
      901 ttttaaaagg cggttccgtt ccttcttttc tcagaatatt gtattgctcg ggagtgagga
      961 gcgagcgcca gtcatcgtct gttttggtga tctcaaactt ttcgtcaagc tccccggctc
     1021 gaacgagcag aggctcgaaa gacccggcaa aaattccgaa tcccacggcc atagcagatt
     1081 taaagaaagt tcttcgatcc atcgtccctc cttatcgtcg gcttttaaaa catgatttat
     1141 ctaaccaaag gtgaaggcga aaagccttac ttttggtttt tcaaagatca gttcaaagag
     1201 atggtccgct tcatcgcccg gcggaagcgt aacgaggttg tagagcctgg aaccgctgat
     1261 ggcaaccttg ccgtccggtc ctacatcgtc tccgcgtgag tcgagaggaa tcggtttccc
     1321 atcgagtaaa acttttgccg tcgatccttt ttcttcctcc gccatcacca gattgagctt
     1381 tggcgcttcg aatcgaaatc gaatcgcacc gtgggaagct tgtagaaccg cggcctcttc
     1441 cgtaattttc cacaatcccg tgagcgtcca ctggttgaga ccgagtgccg taggcgcgga
     1501 gtaggtcagg acggcgtccc gttgcaggca ttgaggtgat gagaaattct gggcacggcc
     1561 atacccgata taagtctcga acgaatgaat ttttgaaaaa tctaccgaac ctttgggggc
     1621 ttccaggttc acgggcttat gaagtagctt cgcctcggcc aaaagcgcct ggatggcctg
     1681 ttcggtctcc tcataattgc cctcgccaaa atgttcttga cggatcctcc cccgggcgtc
     1741 aattaagtag tgcgccggcc aatagcggtt gttgtacgcg ttccaggctc cataatcatt
     1801 atccacggcg accgggtaat ggacaccatg ccggatgacc gcctgcttga tattagatac
     1861 ctctttttca aaactgaatt ccggcgtgtg gacaccaacg atgaccagcc cctgatcctt
     1921 atacttttcg taccactgag tgacataagg gagagttcga atacagttga tgcaggaata
     1981 ggtccagaaa tcgactagga caactttctc tctcagacct gatgaggaca tcgccggaga
     2041 attaatccaa gcggtaattt gtgaaaactc cggcatcgtt cctaaaacgg gtaacggcac
     2101 agcttcggtc gatgccaggg cgatgccgga tttcgaatcg gtctctggag acacagagtc
     2161 tttcttttca accaattttt cttcaatcca gttagcaata aaaagttgtc cgggaagatg
     2221 agagagaacc agcgcatcaa cgttaaaata gagtccgatg accgtgacga gtgttagaaa
     2281 acccaggacc ttatgggaaa ttggtcccca ggcgcgaaac ctctgaattc ggccggtaac
     2341 cctccgtccg ccataaccaa tcagcaacat cgggacagcg gctccaagag aataccctcc
     2401 cattaggacc agggtttgtg taaacgatct ctgaacggtt gccagcgtaa tgataatgcc
     2461 gagaatcggt cctgcacagg gtgcccagat cagtccaagc gataccccga tcaggaaggc
     2521 gttcagccgc ccttgctccg taatgagctt gccggttccc gtaattcttt gggcaaggga
     2581 actgatccgg tttcccattc cctcccagag ccttggccgg atcaaggaga ggccgaagaa
     2641 aaatagaacg accagggccg cttgacggag gaaagaactg ctcaaaccca gaaagcttcc
     2701 aaaaagagca aaggtcgttc cgaagaaggt gaagccgctt gcgagtccca acacgatcca
     2761 aagaggacgg tgacgaaccg gagtcgcaac ggaagcgccc agaagaggcg gaagaaccgg
     2821 aaggatgcaa ggactgacca cagtcagcaa tccggcaaga attgcgaata gctccaatgc
     2881 ccagttcatg ttttactcct tttcacccgt tttgctaaat gggttagatt tatttttaat
     2941 ggaaaaaatg aatttaattt acgggttgat cttaccgaag ggatttgaag aaagaatgaa
     3001 ggaaatgtga agattcgatg aagatcggtt caaagaggaa gggtaaacca gatctcggtc
     3061 cggccgggcc ggctgtgcgc tcctacggtt cccccctgga cttcgataat ctgttttacg
     3121 atcgccagcc cgatgcccgc tccgcccgat tcgcgggaac gggatttttc acctcggtaa
     3181 aaccgctcga aaatgtgcgg aaggtcttcc tcacggattc cttcaccgct gttgctgaat
     3241 cggacctcca ctcgaccccc gtctattcct atatcgacgc cggcctctcc tccatcggga
     3301 gtgtactgca gaacgttttg aatcaggttc tggaccacct gaatcatctg atccgcatcc
     3361 gcggacacgg caacgggcgc tccgttaatc ttcagccgga ttctctgacg gtcaaagcgc
     3421 gcctgctctt tctgaagcag ttgaaggacc agcgcctgta ggtcaaactg ctcctttgtc
     3481 acgacttgtg tttcggcatc gatctgagaa agacgatgga gcgcctcgac cagacgaacc
     3541 agacggagga cttcttcatg aagggaatcg accgtttccc gtgacggttc aattaactgg
     3601 tcttgaatcg cctccagctg gccccgtatg ttattcagcg gggtccgaag ctcgtgggcc
     3661 acattggcaa ccagatcttt cctcatcctc tcgattttac cgaggctgtc gaccatttga
     3721 ttaaaggcct gtcccaattc gccgacctcg tcgcgcgtcg ttaccctgac ccgctcggag
     3781 tagtcccccc tcccaagctt ctgcgtgatc ttattcatct gtgtgagggc gcgcatcaac
     3841 tgtttggtca tcaagaagct gacaagcaat accaagccga gtccgagaaa catcgaaaga
     3901 agtagaaatt gatgtgtggt atgaaggaac atcccgtgaa ggaccttggg atcgatgttg
     3961 tactgcttca tgaggtccat aaaatagtcg gcggcaaaat gatcgatgac ctgccaggcc
     4021 aggaagatga cgattccaac tcccagcagg ttgaccgcaa cgagtttcca gatcaggctg
     4081 atttttgggg ttcgcattct atttttccgc aaattgataa ccgattccgc ggaccgtttt
     4141 aatataccgg ggccgtgccg ggttcgtttc aaccttttgc cggagattct tgatatgcac
     4201 gtcgatcgtg cgatccagta cattgatctc gcttcttgtg taaataatgt ccagaagcgc
     4261 ctccctcgaa aagacccggc cgggcgcggc catgaacgtc atcagcagct taaattcaag
     4321 atgggtcaat cttaacgggt tcccgtgcaa actgactttg tgtttttcag gatcgagaac
     4381 caatccgagg aacaagatcg gctttgtcgg acccgtctct tttcgccgga accggcggag
     4441 gatgaccttg acccgtgcca ccacttctct cgggctgaag ggcttcacga cgtaatcatc
     4501 ggctccgata gagagcccga ccagtttatc gacctcctcc accttggcgg tcaccatcat
     4561 gatcggtaca tccgactcct gacggatctt cttgcacacc tcg
//
LOCUS       DATMKH010000143         1013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1351385, whole genome shotgun sequence.
ACCESSION   DATMKH010000143 DATMKH010000000
VERSION     DATMKH010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1351385"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..982
                     /locus_tag="VNV63_01795"
     CDS             86..982
                     /locus_tag="VNV63_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXC61383.1"
                     /translation="MSEAQPPGTAVSSGNRPSQAAIEVKGLEMGYGSFVLMRDIEFSV
                     ASGQIMVIMGGSGCGKSTLLKYLIGLKEVEKGEIIYYGRGFSKASPKEKEEMQRTFGV
                     LYQGGALWSSLTLAENVALPLEQYTTLSGKEIMEIASMKLALVSLKGFEEFYPSEISG
                     GMRKRAGLARAMALDPAFLFFDEPSAGLDPITSVRLDDLILRLRDCLGTTIVVVTHEL
                     PSIFAIADTSIFLDAETKRMIAHGNPRELVKEGKSDPKVRNFLLRCDPEKTAIGLARD
                     DPSANNRDSTDHGQISSKAGIQ"
BASE COUNT          246 a          246 c          282 g          239 t
ORIGIN      
        1 cgatgtgatc caggaagtat tgaatctgta aacgtcttga tattttgccg agatcccctt
       61 tgccttgaat ccaatcgaga atccaatgtc agaagcgcag ccgccgggta ccgcggtttc
      121 ctctggaaac cggccttccc aggcagcaat cgaggtcaaa gggcttgaaa tgggctacgg
      181 ttcattcgtc cttatgcggg acattgagtt ctcggtagcg tctggccaga tcatggtcat
      241 catgggcggg agcggctgcg gaaagagcac tctccttaaa tatctcatcg gacttaagga
      301 ggttgagaaa ggagagatta tttactatgg acgaggtttc tctaaggcct ccccgaagga
      361 gaaggaagag atgcaaagaa ccttcggcgt gttataccag gggggtgcct tgtggagttc
      421 gctcaccctg gcagaaaacg tcgctctacc tttggagcag tatacgacgc tttctggcaa
      481 ggagatcatg gagatcgctt ccatgaagct cgcacttgtg agcttgaaag gattcgaaga
      541 attttatccc tcggaaataa gcggcggtat gcgtaaacgt gcgggtctcg caagggcgat
      601 ggcgcttgat ccggctttcc ttttctttga cgaaccatct gcggggctgg acccaatcac
      661 ttccgtacgg ctagatgact tgatcctgcg gctccgggac tgtctcggta cgacgattgt
      721 cgtggttacc catgagctcc cgtcgatttt tgccatcgcc gacacgtcta tttttctcga
      781 cgcggagacg aagagaatga tcgcccatgg caaccccaga gaattggtca aagagggaaa
      841 gagcgatccg aaagttcgca acttcttgtt gcggtgtgac ccggagaaaa cagccattgg
      901 tctggccaga gacgatccat ccgcgaacaa tcgcgattcc acagatcatg gtcagatcag
      961 ttccaaagca gggatccaat gagtcaaaaa gcgaatccta gcttgattgg agc
//
LOCUS       DATMKH010000144         1328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2956420, whole genome shotgun sequence.
ACCESSION   DATMKH010000144 DATMKH010000000
VERSION     DATMKH010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2956420"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1119)
                     /locus_tag="VNV63_01800"
     CDS             complement(<1..1119)
                     /locus_tag="VNV63_01800"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HXC61384.1"
                     /translation="MKKASRPISAKDVCAALTLLGIAVLFLLALAPSAFAQELSSKDY
                     RDIPYIGSRNAVWIIAQIHLLFGGFVLGVPIFALVCEIIGYITKDKRYDTLAKEFTKL
                     LTASFGTTATLGGILLFLLIVCYPKLFNYLTEVFYPTYFFYSLLFIGETISLYLYWYG
                     WDAMAGKKSLHIFLGFLLNIFGLLIMFTSDAWLTYQASPAVVSGGASFADKLWASIDN
                     PTWGPVNIHRFIGNIVLGGFMCGAYAGLRYLGAKSQEERAHYDWMGYIGNFVGIFGLL
                     PLPFAGYWLMREIYQYNQQMGITLMGGFLSWLFILQAILIGVLFLGANYYFWQGLLIR
                     AGGGLRYKTYITIMLVVLLILLGIWMTPHSLVASMQEAR"
BASE COUNT          353 a          352 c          343 g          280 t
ORIGIN      
        1 tcttgcctcc tgcatactgg ccaccaggct atgaggcgtc atccaaatcc caagtaagat
       61 caagagcacg acgagcataa tcgtgatata ggttttatac cgcaagccgc ctccggctct
      121 tatcagaaga ccctgccaga agtagtagtt ggcaccgagg aaaaggacgc cgatgagaat
      181 cgcctggagg ataaacaacc aggagaggaa tccccccatg agcgtaatgc ccatctgttg
      241 attgtactgg tagatctcac gcattagcca atagcctgca aacggaagag gcaataggcc
      301 gaagatcccg acaaaatttc cgatataccc catccagtcg taatgcgccc tctcctcttg
      361 cgactttgcc ccgaggtacc ggaggcctgc gtatgcgccg cacataaacc cgcccagtac
      421 gatattgccg atgaagcgat ggatattcac cggcccccag gtcgggttgt caatcgaggc
      481 ccagagcttg tcggcaaaac tggcgccgcc ggataccacc gccgggctcg cttggtaagt
      541 caaccaggca tcggacgtga acataatgag aaggccgaag atattgagga gaaaacccag
      601 aaaaatgtgg aggcttttct tcccggccat tgcatcccac ccgtaccagt aaaggtagag
      661 actgatcgtc tccccgatga agagcaggct gtagaagaaa taggtcggat aaaaaacctc
      721 ggtgaggtaa ttgaacaact ttggatagca gacgatcaga agaaaaagga ggatgccgcc
      781 caaagtggcg gtagtcccga aggaagcggt caagagcttg gtgaattcct tggccaaggt
      841 atcataacgt ttgtccttcg taatataccc gatgatctcg cagaccagcg caaagatggg
      901 aacacccagg acgaacccgc cgaagagcag atgaatctgt gcaatgatcc agacggcatt
      961 acggcttccg atatacggaa tatcccgata gtccttggag gacagttctt gggcaaaggc
     1021 cgacggcgct aaggccagca gaaagagcac tgcgatgccg agaagggtaa gagccgcgca
     1081 gacatctttc gcggatattg gccttgatgc tttcttcaaa taaatgtcta tgagctccct
     1141 ctttcaaatc aatccgcctc aggcggaggt cgtatcggtt aaaccgtcac tttccgattt
     1201 cagggtgctc gtgaatttca gcttttcccc catgggagaa ggtgtgctcg taggccatca
     1261 cctgccacct ttgctcctct gtcaagtcat ccttccagga tttcatcttg gttttaggca
     1321 cgccttcc
//
LOCUS       DATMKH010000145         2001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4238726, whole genome shotgun sequence.
ACCESSION   DATMKH010000145 DATMKH010000000
VERSION     DATMKH010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4238726"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VNV63_01805"
     CDS             <1..265
                     /locus_tag="VNV63_01805"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HXC61385.1"
                     /translation="EQSLPLLKQQQGFVDALILSSDTDRDQFVSVTIWKSREDAERYV
                     NSQARQELEAIRALVQHEPTFRTFNLEASTIHGVGIGRAAASR"
     gene            736..1410
                     /locus_tag="VNV63_01810"
     CDS             736..1410
                     /locus_tag="VNV63_01810"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HXC61386.1"
                     /translation="MDEALSSALMVQDRELASILQEVEKISASLKSEAPDTRSLSETL
                     QRTLQCAVKRSFLERELRSLALTDDLTGLNNRRAFMALAGQQLKVARRSEQGLLLFFA
                     DVDNLKEINDTYGHQEGDLALIHAADALERTFRDSDVIARLGGDEFAVLALEASCENR
                     DAILRRLEKNLRESNRNESRYQLSVSVGMSRFDPNHPVPLGKLIANADEAMYEEKKSR
                     LKLCVT"
     gene            complement(1667..>2001)
                     /locus_tag="VNV63_01815"
     CDS             complement(1667..>2001)
                     /locus_tag="VNV63_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61387.1"
                     /translation="YEPSRLRPFGFTGGLAIMKKTETGRSTFAVISLWGKFGGLHTCG
                     ATFRYIVALQNIFLTRLFSKLRLKRKDFQEILRLSWAQEASGSNPDAPTKLFSESPSG
                     IIFGNTSM"
BASE COUNT          491 a          500 c          552 g          458 t
ORIGIN      
        1 tgagcagagc cttccgcttc tcaagcagca gcaaggattc gtggatgctt taattctatc
       61 ctcagacacc gatcgggatc aattcgtgag tgtcacgatt tggaagagtc gagaagacgc
      121 ggagaggtat gtgaacagcc aggcgcgaca ggagctggag gcgatccggg cactagtcca
      181 gcatgaacca acttttcgca ccttcaatct ggaagcctcc acgatccatg gcgtgggtat
      241 tgggcgcgcc gcggcgtcca ggtagggaga ggcgggagtg aaatcccacc cagccctcga
      301 tcttcatttc cgcacccggc ttctcaaaat caattggcgt cgccaatggg agctgcgccc
      361 ggttttttct tgaagggcca aagacgataa aaatgtgact ggccacgaaa aatgtgttca
      421 ttcgtgcaat accaggtact aagggatccg cagcttccac tctacagcgt ttacttgatg
      481 gttagcgtcg acgtgaatga ggtttaatac aggtgctctt ttatgttccg tccagtaaat
      541 taatacagga agatggtgtc cgatccgatg agccgaaaca ctttatatct gtgggaaaga
      601 ggaacgattc aaacggacag tttgtgtacc gacgaaatca gacaggctgt ccgctcacga
      661 ataccagcag agggccatgc ctccgcacgc aggattttca gtttggatgg ctggagtcca
      721 agtttggagg attccgtgga tgaggcgctt tcctcagcgc taatggtcca ggacagagag
      781 ctggccagca ttcttcagga ggttgagaag atttccgcga gtttgaagtc agaggctccg
      841 gacactcgga gtctcagcga aacccttcag cgcacgctcc agtgtgccgt taagcggtcc
      901 ttcctggaaa gagagttacg gtctctagcc cttacggacg atcttacggg tctcaataac
      961 cggagagcgt tcatggcttt ggcagggcaa caattgaagg tagcgcgacg gagtgagcag
     1021 gggttattgc tcttctttgc cgacgtcgac aatctcaagg aaattaatga tacctatggg
     1081 catcaggagg gagacctcgc cctcatccac gcggcggacg cattggagcg gactttccgc
     1141 gattccgacg ttatcgcacg ccttggcggc gatgaatttg ccgttttggc tctagaagcc
     1201 tcctgcgaaa accgggatgc catcctgcgc cgcttggaga aaaatcttag ggaatccaac
     1261 cggaacgaat ctcgctacca gttgtctgtc agcgtgggaa tgtcacgatt cgatcccaat
     1321 catcccgttc cacttggaaa actcatcgcg aatgcagatg aagctatgta cgaggaaaag
     1381 aagagccggc tgaaattgtg cgtgacttag agattatgag aaggaaatga gacgatccgg
     1441 ctggaatcgt ggaggggccg gaaagatctc cggggtagct acactttcca ccgcaatgca
     1501 atgaacgcga aatccctgta gcagttgggt gtggacctgc aaaaaacgac cagctgcccg
     1561 ccgcgagaac gcgtcgcctc tgcaaattca tctgcgacaa atattgacgg atttgtatcg
     1621 acctctactc cctgtgccct ccgggatggt gagagcacgt tccgattcac attgaagtat
     1681 tgccgaagat gatgccgctc ggtgactcgc tgaaaagttt ggtcggggcg tccggatttg
     1741 aaccggaggc ctcctgcgcc caagacaggc gtaatatctc ctggaagtcc ttccttttca
     1801 atctcagttt tgaaaacaaa cgagttagaa aaatattttg cagtgccacg atgtaccgaa
     1861 atgtggctcc gcatgtatgg agtcccccga attttcccca tagcgaaata acagcgaaag
     1921 tgcttcgacc ggtttcggtt tttttcataa tcgccaaccc tccggtgaag ccgaaggggc
     1981 gcagtctact cggctcatat g
//
LOCUS       DATMKH010000146         2602 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2637251, whole genome shotgun sequence.
ACCESSION   DATMKH010000146 DATMKH010000000
VERSION     DATMKH010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2637251"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /locus_tag="VNV63_01820"
     CDS             <1..700
                     /locus_tag="VNV63_01820"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HXC61388.1"
                     /translation="ADFNHDHHLDLVVAVRTDKIQIYLGQGNGKFVKKTVFDPGDTPT
                     SVKVWDFNHDGQLDLAVANNGAIGNNLSIFFGKGDGTFVLSKSYETKLRPLSLTLADF
                     NNDKEEDIVVVNGIRSTLTLFLGNKDGTFQDGIPFGAEGGPLAAAAGDFDGDHKLDLA
                     VTNDLSNNLSILLGKGDGTFLQPPINYRTGEGPFAITTGDFSHSGKQGLAVANNEGNS
                     VSVYLPRLNPPVIK"
     gene            complement(716..1186)
                     /locus_tag="VNV63_01825"
     CDS             complement(716..1186)
                     /locus_tag="VNV63_01825"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HXC61389.1"
                     /translation="MKRITPGWVILGMLVLIGFYAWFLLRGGGEPSHFILTFPDGTRV
                     VAEVADSDAERLLGLIFKKKLPDNMGMLFIFDEPDYHRLWTKNVRFPIDLIWLDPDRR
                     IIGWEENLLPCRQDPCPTYAPKQQRALYAVEIPAGFVKQHDLKTGMQVVFDLPK"
     gene            complement(1183..1506)
                     /locus_tag="VNV63_01830"
     CDS             complement(1183..1506)
                     /locus_tag="VNV63_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002624077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HXC61390.1"
                     /translation="MPKFLHHIFICENRRDPSDSKGCCAAKGSEELRKVFKQEIERLG
                     LKGRVRANKAGCLDACAFGPSVVIYPEGVWYRVRTPEEVREIMECHIRDGKVVRHLLM
                     PSGDK"
     gene            complement(1539..2150)
                     /locus_tag="VNV63_01835"
     CDS             complement(1539..2150)
                     /locus_tag="VNV63_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61391.1"
                     /translation="MKILLPFILSFFLTGCSTTYLMGYHYLGLEDEQFVTGKVSKKTE
                     KQSKPFEEKCIQVLMNADMDSFKELFTDKLLKAVPKDAYIKIKEAIQIRYKPNGQYER
                     LPVLHMHTEHKHELDTATLFNGFDHYDYIEAEYLLYGATDAVVHLYLTEVGKNPKTPK
                     LSGFELTDADSSKQERKFSIKYLVPETVDKAGLIGTEILKLPK"
     gene            complement(2228..>2602)
                     /locus_tag="VNV63_01840"
     CDS             complement(2228..>2602)
                     /locus_tag="VNV63_01840"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXC61392.1"
                     /translation="QQASIELTDRGRQLLETGRWEEATSVFQKAISLFPSNPYPYYYL
                     AKARYMQQEYSKVLPFLGQAERFFGGDPIWLSRVYVLRGRTYEALSRFDEAKDQYRQA
                     LSRDPGNPTAQEGIERINNKLN"
BASE COUNT          642 a          680 c          648 g          632 t
ORIGIN      
        1 cgccgatttt aaccatgatc atcacctgga cctcgttgtg gcggtgcgaa ctgataagat
       61 ccagatctac ctcggacaag gcaacgggaa atttgtcaag aaaacggtct ttgatccggg
      121 cgatacgccc acatcggtga aggtctggga ctttaaccac gacgggcagc tcgatctggc
      181 ggttgcgaac aacggtgcga tcggaaacaa tctcagcatc ttttttggaa aaggggacgg
      241 gacctttgtc ctctctaaat cgtatgaaac aaaactccgc cctctctcgc tgacgctggc
      301 ggatttcaac aatgataagg aggaagacat cgtggtggta aacggaattc gaagcacact
      361 gacactcttt ttagggaata aagacggaac ctttcaagac ggtattccat ttggtgcgga
      421 agggggaccg cttgcggctg cggcaggaga ttttgacggc gatcataagc tggatctggc
      481 ggtgacgaat gacttgagca acaacctctc catactgtta gggaagggcg atgggacctt
      541 cctccagccg cccattaatt accgtaccgg agaaggacct tttgcgatca cgaccggtga
      601 cttcagccac agcggaaaac agggtctggc ggtggccaac aatgagggca attcggtctc
      661 ggtctatctt ccccggctta acccgcccgt gatcaagtaa gggaaccgtt cacctctact
      721 tgggcaaatc aaagacaacc tgcatgcctg tcttcaaatc atgctgtttg acaaagccgg
      781 ccgggatctc aactgcatag agcgcccgct gttgtttggg agcatatgtg gggcaggggt
      841 cttgacggca cggcaagaga ttctcttccc agccgataat acgccgatca ggatccagcc
      901 agataagatc gatcggaaac cgtacattct ttgtccataa gcgatggtag tccggctcat
      961 caaaaataaa cagcatcccc atgttatcgg gaagcttctt cttaaagatc aatccaagta
     1021 acctttcggc atcactgtcg gcgacttcgg ccacgacccg ggtcccatcc ggaaaagtga
     1081 ggataaaatg gctcggctcg cctccgcccc gcaacaggaa ccaggcataa aagccgatca
     1141 aaacgagcat ccccaagatg acccagcctg gcgtgatcct cttcatttat ccccggaagg
     1201 catgaggaga tgccgtacca ccttcccgtc ccggatgtgg cattccatga tctcgcgtac
     1261 ctcttccggc gttctcacac ggtaccagac gccttcggga tagatgacca ccgagggacc
     1321 gaaagcacag gcatcgagac atcccgcctt gttggcccgc acacggcctt tcagccccag
     1381 gcgttcgatc tcttgtttaa ataccttccg cagctcttcc gatcctttgg cggcacagca
     1441 gcctttcgaa tcgcttgggt cacggcgatt ctcgcagatg aagatatgat gaagaaattt
     1501 cggcatatat gcttattgag ggaagagatt ttcttgtctt atttcggcag ctttaaaatc
     1561 tctgtcccga tgagtcccgc cttatcgacc gtttcaggaa ccagatattt tatggagaac
     1621 tttctttcct gtttactcga atccgcatca gtcagttcga atccggatag ctttggagtc
     1681 ttcgggttct tcccgacttc ggtcagatac agatggacta cggcgtccgt agcgccgtat
     1741 agaagatatt cggcttcaat atagtcataa tgatcaaacc cattaaaaag agtagccgtg
     1801 tccagttcat gcttgtgttc cgtatgcata tgcaaaaccg gtaatcgctc gtactgaccg
     1861 ttgggcttat atcgaatctg tattgcctct tttattttta tataggcgtc ctttggcaca
     1921 gccttcaaca acttatccgt aaacagttct ttgaatgaat ccatgtcggc attcataaga
     1981 acctggatgc acttctcttc aaacggtttg gattgcttct ccgtcttttt agacaccttg
     2041 ccggtaacga actgctcatc ttcaagacca agataatggt atcccatgag gtatgtggtt
     2101 gaacaaccgg tcagaaaaaa ggaaagaatg aagggcaata gaattttcat ttgggtcgaa
     2161 gcagtgatat accagatcgg tcgccggaag acaagtagaa aaaacctgcg ccggataact
     2221 tcccgattca atttagttta ttgtttatcc tctcgatccc ttcctgggcc gtcgggttgc
     2281 ccgggtctct ggacagggcc tgccggtact gatcttttgc ttcgtcaaaa cgggaaagcg
     2341 cctcgtaagt tcttccacgc aaaacataca cgcgcgacag ccagatcgga tcgcctccga
     2401 aaaagcgctc ggcctggccc aaaaagggga ggaccttcga gtattcctgc tgcatataac
     2461 gcgccttggc gagatagtaa taaggatagg ggttggaagg gaaaagcgag atcgccttct
     2521 ggaagaccga tgtcgcttct tcccaccggc ccgtttccag cagctggcgt ccccggtcgg
     2581 tcaactcgat cgaggcctgc tg
//
LOCUS       DATMKH010000147         1133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_712364,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000147 DATMKH010000000
VERSION     DATMKH010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_712364"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="VNV63_01845"
     CDS             <1..618
                     /locus_tag="VNV63_01845"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXC61393.1"
                     /translation="WESYLAGLGQSHRNNFKRRLKNLTKEFDVQFEISDSEERRHGAL
                     KILIDLHNMRWKGRGTSSAFHTSGVISFHEDLSRLALERGWLRLYVLWLNGRPAASLY
                     GFKYGPVFYFYQSGLDPQFEKQSVGLITMGLAIKGAIEECVKEYDLLHGDEQYKFLWA
                     HEVHELSRLELYPPQIRGLLYRQTMGWLRASRRVAKHVISRTLAR"
     gene            632..>1133
                     /locus_tag="VNV63_01850"
     CDS             632..>1133
                     /locus_tag="VNV63_01850"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HXC61394.1"
                     /translation="MTKGQMVIGNNSKLRSLIKTGLSCGLHWTGADKLIGSFGGYRNT
                     PLVIGYHRVVEDLPAYGGNGVPRMIISRRTLERQLDWIGRRFRFISLDELGEKLECGE
                     KFDKPVAVITFDDGYSDIYYNAFPLLKQKGIPAGVFVVTDLIGASRLQIHDKLYLLLE
                     CAFSLWG"
BASE COUNT          263 a          262 c          315 g          293 t
ORIGIN      
        1 tgggaatcat atctggccgg tcttggccag tcgcatcgca ataacttcaa aaggagactt
       61 aagaacctga ctaaagagtt cgatgtgcaa ttcgagattt ctgattcgga ggaacggcgt
      121 catggggcgc taaaaatact catcgatcta cacaacatgc gctggaaggg gcgaggtacc
      181 tcttccgctt ttcatacctc cggtgtgatt tcctttcatg aggatttaag ccggttggca
      241 ttggagcgtg gctggctgag gctgtatgtc ctgtggttga acggaagacc ggccgcgtct
      301 ctctatggtt ttaaatacgg tcctgttttt tacttctatc aatcggggct tgatccacag
      361 tttgagaagc agagcgtcgg cctcattacc atgggactcg ccatcaaggg ggccattgag
      421 gaatgtgtga aagagtacga tctgcttcat ggcgatgagc agtataagtt tctttgggcg
      481 catgaggtgc atgaactgtc gcgcttggag ctttacccgc cccagatacg cgggttgctt
      541 tacagacaaa ccatgggatg gctccgcgct tctcgtagag tggcaaaaca tgtcatttct
      601 cgaacgctcg cccgatgaga tcgctttccg aatgacaaaa ggacaaatgg taatcggaaa
      661 caattcaaaa cttcgcagtc tgattaagac agggctatcc tgcggtttac attggaccgg
      721 ggcggataag ctcatcggtt ccttcggagg ctatcgaaac acgccgttgg tcatcggtta
      781 tcacagagtg gtggaggatc tcccggcgta tggaggaaat ggcgtcccaa ggatgataat
      841 cagtcgccga acattggaac gacagctgga ctggatcggg cggcggttcc gcttcatctc
      901 tctcgatgaa ttgggcgaga agctggaatg cggagaaaag tttgacaagc cggtggctgt
      961 catcaccttt gacgacgggt actccgatat ttattacaac gcattcccgc tgcttaagca
     1021 aaaagggatt cccgccgggg tcttcgtggt cacggatctc atcggagctt ctcgcttaca
     1081 gatccatgac aagctttacc ttttactcga gtgcgctttt tcgttatggg gtt
//
LOCUS       DATMKH010000148         1378 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1035940, whole genome shotgun sequence.
ACCESSION   DATMKH010000148 DATMKH010000000
VERSION     DATMKH010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1035940"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            334..876
                     /locus_tag="VNV63_01855"
     CDS             334..876
                     /locus_tag="VNV63_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61395.1"
                     /translation="MSATNYFEELYGVTEERALNFWVSHLEDTIGSEQWSQIRDSTGV
                     YLASVLGHFCLQSVDRMETLSPDYSPSDQEFRQFADLTQIAELMMRQLTSAKNPMWME
                     SAGAHILLYAGFFQDQHKDRHNIGFYSTMGKECYLMAAVGKRAKIMKLMAAGFSEYLF
                     HLATLHRNLQESRYLIRPVN"
     gene            complement(873..1262)
                     /locus_tag="VNV63_01860"
     CDS             complement(873..1262)
                     /locus_tag="VNV63_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXC61396.1"
                     /translation="MSVWYSRPVLFVESVERSIAFYIEKLGFTESTRYEEEGKILVAQ
                     VDREDCEVLLNCQQPEKIGRSRIFISIDLEPLQALRNEFEQRGAPIRDGWWGYDTMII
                     EDPDGNELFFPYPKNNAKEKSDPVSGS"
BASE COUNT          317 a          331 c          389 g          341 t
ORIGIN      
        1 taccagcatg gcgggattct gcagtatgtt ttgcggcagt tggcggggaa ggctccggct
       61 tcggcgtagc tgatccccta cttccttttc caaaggcacc cttatccggg gtgcctttgg
      121 ttttaggcaa acctattatt atcaatggat tgcgcaggat gatccctttg agggattgag
      181 aggggataac tgtatccgca aaatgccagt cagattaact gagagggggc gtatccagct
      241 ggatgcggcg atcactcaag aatttttcac gcgacaggcg agacaacgat aaaagatagg
      301 aaattcaatt ccattcccag gcggttggcg aatatgtcag ctactaacta ttttgaagag
      361 ttatatggtg tcacggaaga acgggcgctt aacttctggg tctcgcatct ggaggatacg
      421 atagggagtg agcagtggtc ccagattcgc gactcgacgg gtgtctatct cgcaagtgtg
      481 cttggacact tctgcctgca atcggtggat cggatggaga ctctgtctcc ggattacagc
      541 ccgagcgatc aggagttccg gcagttcgcg gatctgaccc agattgctga actgatgatg
      601 cggcagctca cgagcgcgaa gaatcctatg tggatggagt cggccggtgc gcacattctt
      661 ctttatgccg gcttctttca agaccaacac aaagaccggc acaatattgg gttttattcg
      721 accatgggca aagaatgcta tctgatggcg gcagtcggaa agagggcgaa gatcatgaag
      781 ttgatggcgg cagggtttag tgaatacctg ttccatctgg ctacgcttca tcgaaatctg
      841 caggagagca gatatctgat acggccggtg aactaacttc cgctgacggg atctgatttc
      901 tctttcgcat tgttcttcgg gtaggggaag aacagctcgt tgccgtcggg gtcttcgata
      961 atcatcgtgt cataacccca ccagccatcc ctgatcggcg ctccgcgttg ctcgaactcg
     1021 ttccttagcg cctgaagcgg ttctaaatcg atcgagatga agatgcggct gcgcccgatc
     1081 ttttcaggct gctggcagtt gaggaggact tcgcaatcct ctcggtcgac ctgagcgacg
     1141 aggatcttgc cttcctcttc gtagcgggtc gattcagtaa aaccaagctt ttcgatgtag
     1201 aacgcgatgg accgctctac actttcgacg aacaaaacag gacgcgaata ccagacgctc
     1261 atttaatgga tcctcgcacc ggcgcgatgt tcatcatagc cggaaaggtg gcgctttccc
     1321 tttgagcgac ggcaggtgca gcctggagga tgggaatttc gactagtaag aattactc
//
LOCUS       DATMKH010000149         1067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1673572, whole genome shotgun sequence.
ACCESSION   DATMKH010000149 DATMKH010000000
VERSION     DATMKH010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1673572"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VNV63_01865"
     CDS             complement(<1..188)
                     /locus_tag="VNV63_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein]--UDP-N-
                     acetylglucosamine O-acyltransferase"
                     /protein_id="HXC61397.1"
                     /translation="MSIHPTAIIDPKAELGADVVIGPYCVIGPDVSIGDGCWLQNHVT
                     VCGPTKIGKQNRFHAYCS"
     gene            complement(185..>1067)
                     /gene="fabZ"
                     /locus_tag="VNV63_01870"
     CDS             complement(185..>1067)
                     /gene="fabZ"
                     /locus_tag="VNV63_01870"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HXC61398.1"
                     /translation="SLMTLVPSSGFRVSCTQVGPDGRFTQYFSTEITPEIYEKEIAPA
                     RTFVFYEDVQPLMDKGLIRGGSLENAVVIRGDSIMSKEPLRFQDEFVRHKILDILGDL
                     ILAGCRIAGHVIAVKPGHGANSELTRGLSKRYAQMLAMVPPRVIPQGSAVMDTKDVLA
                     VLPHRYPFLMVDRILSFEGERKIVGMKSVTINEPYFQGHFPGHPVMPGVLQVEAMAQV
                     ASILMSKTNPGANGIGYFMSADEVKFRKPVVPGDTLIIEAELLRTKGTIGKASCRCLV
                     NGEVTSEGILLFAFILQ"
BASE COUNT          262 a          317 c          255 g          233 t
ORIGIN      
        1 atcgaacaat aggcgtggaa ccggttttgt ttcccgatct tggtcggccc gcaaaccgtc
       61 acatgatttt gcaaccaaca gccatcacca atagaaacat ccggaccgat cacacaataa
      121 ggaccaatca ccacgtccgc acctagctcc gctttcggat caatgattgc agtcggatga
      181 atgctcattg caggatgaac gcaaacagca ggatcccttc cgaggtgact tcgccgttaa
      241 ccaggcaccg gcaactggct tttccgatgg tccctttggt ccggagcaac tcagcttcga
      301 tgatcagcgt atcacctggc actactggtt tccggaattt cacctcatct gcactcatga
      361 aatagccgat gccgttggct cccgggttcg tcttcgacat taaaatgctg gcgacctgcg
      421 ccatcgcttc aacctggaga accccgggca tcacggggtg accgggaaaa tggccctgaa
      481 aatacggttc attgatagtc accgatttca tgccgacgat cttcctttca ccttcaaagg
      541 acaggatccg gtccaccatc aggaacggat aacgatgagg taaaacggcc agaacatctt
      601 tggtatccat taccgccgac ccttgtggaa tcactcgcgg aggaaccatg gctaacatct
      661 gcgcgtaacg cttggagagt ccgcgcgtca gctcgctgtt cgccccgtgc ccaggcttca
      721 cggcgatcac gtgaccagcg atcctgcagc ccgccagaat caggtcgccc aggatatcca
      781 ggattttatg ccggacaaac tcgtcttgga accgaagcgg ttccttgctc atgatcgaat
      841 caccccggat cacaaccgcg ttctctaagc taccgccccg gatcaatcct ttgtccatta
      901 acggttgaac atcctcgtag aacacaaagg tacgagccgg cgcgatctct ttctcataga
      961 tctccggggt aatctcggtg gaaaaatatt gggtaaaccg cccatctggt cctacctggg
     1021 tacaagagac ccggaaacca cttgaaggga ccagagtcat caatgag
//
LOCUS       DATMKH010000150         3046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4241016, whole genome shotgun sequence.
ACCESSION   DATMKH010000150 DATMKH010000000
VERSION     DATMKH010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4241016"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1225)
                     /locus_tag="VNV63_01875"
     CDS             complement(<1..1225)
                     /locus_tag="VNV63_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXC61399.1"
                     /translation="MSDTGNAVRREHWWVGVTGYQWLVLIVAWLGWVFDSMDSTIYAV
                     VLQPALHELLGPAASIEGIEWYGGLIFSIFLIGWALGGVLFGIMADYVGRTKTLIFTI
                     LIYSIFTGAAALSDNWWHLAVYRFLTALGIGGEWAAGAALVAETWPEEKRAKAAGVLQ
                     SAWAAGFLIAALANLFLGAYGWRIMFLIGILPAFVSLLVRLMVKEPDRWIKANKIRKM
                     ALQKSQRSDDEQKLLAFSLSRLFGRDLRERTFVGATLAFVAVFGLWGATNWTPTLVRQ
                     LLGSRNLDPAIVNRYVSYAVMSLNAGALVGYLAFGPIADRIGRRPTFFLMCLGSLVML
                     PVTFFTPKDYTLVLWLLPILGFFNNGIFSGFPLYLPELYPTQIRATGAGFCFNVGRVF
                     ASVGPFVKGWLGTIFE"
     gene            complement(1247..1663)
                     /locus_tag="VNV63_01880"
     CDS             complement(1247..1663)
                     /locus_tag="VNV63_01880"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HXC61400.1"
                     /translation="MELKRSYFYLGLLLLTVLTALTSFAAEPLVVQTSSDEIQRAEII
                     ADSYSYTPDHLIFKSNVPVELIIHSKTWLVPHNFMLKDADAGLDIKQDIPAGKSVVVK
                     FTPTRSGKFKFYCDKKLLFFKSHEDRGMKGILEVTE"
     gene            complement(1688..2257)
                     /locus_tag="VNV63_01885"
     CDS             complement(1688..2257)
                     /locus_tag="VNV63_01885"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXC61401.1"
                     /translation="MLWLLSLSLAACSGLPRIVVLHDPLTPEEHVTLGSGYEAQGLTK
                     QAAEEYQAALRKQPDDVTALVGLGNLSYQEGKIKDAERYYRKVLDRDPDHPMANNNLA
                     SVYLSLGKNLAEAEQLARRALVKESPHKAYFWETLASIHLRQGRLAEAQGDLNLADSL
                     APVKDRELREKIAQTSAEITKALARNPQP"
     gene            2452..>3046
                     /locus_tag="VNV63_01890"
     CDS             2452..>3046
                     /locus_tag="VNV63_01890"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HXC61402.1"
                     /translation="MRKRFDDSSCLGRIGYATESPVSSLLSNALTLPARSAYTHPHMS
                     RSFIDFSQTSSATKRITAELFLVGATFIWGGTFVIVKVGLEDISPMLLVAVRFSIAAI
                     LFSLLFYKKIFPMNRAVFRKGSWLGLLLFLGFILQTVGLNDTTPSKSAFITGTLVIFT
                     PIFQLIIEKRAPKVGHILGIIIVTIGLWFLTAPASSLF"
BASE COUNT          711 a          874 c          781 g          680 t
ORIGIN      
        1 gttcaaagat ggttcccagc cagcctttga caaagggtcc tacggaggcg aagacccttc
       61 ccacattgaa acagaatccc gccccggtag cccggatctg tgtcgggtag agttccggga
      121 ggtagagagg gaagccgctg aaaatcccgt tgttgaagaa gcccaaaatc ggcagtagcc
      181 atagaaccag tgtgtaatct ttgggtgtaa aaaaagtgac gggcagcatc acgaggctgc
      241 ccagacacat cagaaaaaaa gtggggcggc gcccgatgcg atcggcgata ggtccaaagg
      301 ccaggtagcc caccaaggcg ccggcattca ggctcatcac ggcgtaactc acataacggt
      361 tcacgatcgc cggatcgaga ttccgggaac ccaacaactg ccgaaccaga gtcggtgtcc
      421 aattggtggc accccacagg ccaaagaccg ccacaaaggc cagtgtggct cccacgaagg
      481 ttcgctccct gagatcccgt ccaaacaacc ggctaagact gaaagctaac agtttttgtt
      541 catcatccga gcgctgactt ttttggagcg ccattttccg gatcttgttt gccttgatcc
      601 atcggtccgg ttccttcacc atcaatcgaa ccaggagaga gacaaaagcc gggagaatac
      661 cgatgagaaa catgatccgc cacccgtaag ctcccaagaa caggttggcc aaggccgcaa
      721 ttaaaaaacc ggccgcccag gcggactgaa gcaccccggc ggccttcgcc cgtttctcct
      781 ccggccaagt ctcggcaacc agcgctgccc ccgctgccca ttctccaccg attccaagag
      841 cggttaagaa cctgtagacc gccagatgcc accaattgtc cgagagtgcg gccgctccgg
      901 taaagatgga gtagatcagg atggtaaaaa tcagtgtctt cgtccgtccc acgtagtccg
      961 ccatgatccc gaagagcaca ccgccgagcg cccagccgat caggaagatg gaaaagatga
     1021 gcccgccgta ccactcgatc ccttctattg aagcggcggg gccgagaagc tcatgcagcg
     1081 ccggttgaag tacgaccgcg tagattgtgg agtccatgga gtcaaaaacc catccgagcc
     1141 aggcgactat caataccagc cattgatacc cggtcacccc aacccaccaa tgctcccgcc
     1201 tcactgcgtt tccggtatca ctcatcaaaa gagattcttt ccatccttat tccgtgacct
     1261 ccaggatacc cttcatccct cggtcctcgt ggcttttaaa gaagagcaac tttttgtcgc
     1321 aatagaactt gaacttgccg gagcgtgtcg gtgtgaattt tacaacgacg cttttcccgg
     1381 ccgggatatc ctgcttgatg tccaggcccg catcggcatc cttcagcata aaattgtgcg
     1441 gaacgagcca tgtcttgctg tgtatgatca actccacggg cacgttcgac ttgaagatta
     1501 gatgatccgg ggtgtaggaa taactgtcgg caatgatctc ggcccgctgg atttcatcgg
     1561 aagaggtttg aacaaccagc ggttccgcgg cgaaggaagt cagagccgtc aggacagtta
     1621 agaggagcag cccgaggtag aaatacgatc tttttaattc catagcgacc tccgcttggg
     1681 ataatgtcta aggctgagga ttccgtgcaa gagcttttgt aatctctgcc gaggtttgag
     1741 caatcttttc tctcaactcc cgatctttca ccggtgccag ggaatcggcc aggttcaggt
     1801 cgccctgggc ctctgcaaga cgtccctgcc ggagatgaat gctggccagc gtttcccaga
     1861 aataggcctt gtgcgggctc tctttcacca gcgcacgacg ggccagctgc tccgcctccg
     1921 ccaggttctt gccaagcgac agatagaccg aggccaggtt gttattggcc atcgggtgat
     1981 ccggatcgcg atccagcacc ttacgataat agcgctctgc gtcctttatc ttcccttcct
     2041 ggtaggagag attgccgagc ccaaccaatg cggtcacatc atccggttgt ttccgaagcg
     2101 ccgcctgata ttcttcggct gcctgcttgg tcaggccctg cgcctcatag ccgctgccca
     2161 gggttacatg ttcttccggt gtgagcggat cgtgcagcac gacgatccgc ggcagaccgg
     2221 aacaggccgc aagacttaaa ctaagcagcc acaacatcca tagcagattc caaaaacgat
     2281 tccgatgcat ctttcggagt ataccgaagt cggatagaaa actcaaaccc gtggcaccaa
     2341 tagattttaa gcaggacccg aaggcaagtg tgaaggagtg tatcctgcgc tcgagaacgt
     2401 tcaagattca aaccactgcc agggttgcag taacggctgc gtccctggcc aatgagaaaa
     2461 cggttcgatg actcaagctg cctggggcgc atcggatacg cgacggaatc cccggtttcc
     2521 agcctgcttt caaacgcatt gacccttccg gctcgttcag cttatactca tccccacatg
     2581 agccgatctt ttattgattt ttcacaaacg agttccgcga ccaagcggat taccgccgag
     2641 ctgtttcttg tgggggcgac ttttatttgg ggcggcacat ttgtgattgt caaagtagga
     2701 ctcgaagata tctcgcccat gcttctggtc gcggtccgtt tctcgatcgc ggcgatcctt
     2761 ttctctctct tgttctataa aaaaatcttc ccaatgaatc gagcagtctt ccggaaaggg
     2821 agttggctgg gactgctcct gttcttgggt tttatcctcc agacagtcgg cttgaatgac
     2881 accactccct cgaagtccgc cttcatcacc ggcacgctgg taatctttac gccgattttt
     2941 caattgatca tcgagaagcg ggcgccgaag gtgggacaca tcttaggtat tatcattgtc
     3001 acaatcggtc tttggtttct caccgcgccc gcttcatcac tttttg
//
LOCUS       DATMKH010000151         1247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1997199, whole genome shotgun sequence.
ACCESSION   DATMKH010000151 DATMKH010000000
VERSION     DATMKH010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1997199"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            12..560
                     /locus_tag="VNV63_01895"
     CDS             12..560
                     /locus_tag="VNV63_01895"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXC61403.1"
                     /translation="MDNAIKFTPANGVVKVQSRVFDEDSNLIILEVSDSGCGISRDMT
                     ERIFERLFQASDPDLAGRKGLGLGLFICKELVTRQGGQIWAKSAPGEGAIFSLTLPIC
                     SLSNLIAPLLKDEGWPSDSVALMSVEISSPYGWPSKERREEWSRESRTLVQTCLLPDL
                     DILLPKMDSEGTREVFYRGFCQ"
     gene            774..1184
                     /locus_tag="VNV63_01900"
     CDS             774..1184
                     /locus_tag="VNV63_01900"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXC61404.1"
                     /translation="MSNKKILIIEDDPHVRLGYHVRLQANNYDAFFAADALASMTEAL
                     KHQPDLIILDLGLPGGDGFVVMERLKANTCLAVIPIIVVSGRDPSANRRRALRAGAKA
                     FLQKPVDNAELLAAIRQCLDETNGLRQQDVIPQA"
BASE COUNT          310 a          320 c          334 g          283 t
ORIGIN      
        1 tcatcatcct aatcgataac gcaatcaagt tcacgccggc aaacggcgta gtgaaggttc
       61 aatctcgtgt tttcgatgaa gattcgaacc tcataattct ggaggtttca gactcgggat
      121 gcgggatcag tcgggacatg accgaacgga tttttgaacg cctattccag gcatcagatc
      181 ccgatttggc aggtcgtaag ggactcggcc tggggctatt catttgcaaa gagttggtca
      241 cgcgacaggg cggacagatc tgggccaaga gtgcgccggg agaaggcgcc attttttccc
      301 ttacattgcc tatttgttct ctatctaact tgatcgctcc gctgctcaag gatgaagggt
      361 ggccatcgga ttctgtcgcc ctgatgagcg tggagattag ctcaccgtac ggctggcctt
      421 ccaaggaaag gcgcgaggaa tggtctcggg aaagccgtac tctcgtccaa acatgtttac
      481 ttccagattt ggacattctt ctgccaaaaa tggattctga aggaactcgg gaagtgttct
      541 atcgtggctt ttgccaatga taagggcgcg gcaatacttg ccaaacggat ccgagagcaa
      601 ttcgggcatc tcgaacacct taaacacgaa ggcctgaatg tttcagttac ttataaattt
      661 ctgagtccga ctcctggcga tgtagccgcg ccgtcagagg acactacggg taagatggcc
      721 tcaagaatcg aagaacttat cgcatcggaa tccttagaga gggctgtcat atcatgagta
      781 acaagaaaat tctcattatc gaagacgatc cgcacgtgcg cctggggtac cacgtccgcc
      841 tccaggcaaa caattacgac gcgttcttcg cggccgatgc acttgccagc atgacggaag
      901 ctctcaagca ccaaccggac ctgatcatcc tcgatctggg cctaccaggc ggcgacggat
      961 ttgttgtcat ggagagacta aaagcaaaca cttgccttgc cgtaattcct atcatcgtcg
     1021 tgtctggccg cgacccaagc gcaaaccggc ggcgggctct tcgggccggg gccaaggcct
     1081 ttctccaaaa gccggtggat aatgccgagc tcctagcggc tattcgacaa tgtctggacg
     1141 aaaccaatgg attaaggcaa caggatgtca taccacaagc gtgatggcca atcggacgca
     1201 cgggatttcc atgaccagca atgacactga tcatgcgtgc agaggag
//
LOCUS       DATMKH010000152         1867 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4242724, whole genome shotgun sequence.
ACCESSION   DATMKH010000152 DATMKH010000000
VERSION     DATMKH010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4242724"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="VNV63_01905"
     CDS             <1..267
                     /locus_tag="VNV63_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009381597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HXC61405.1"
                     /translation="VFRDNLLVTELRPQKRLYTESQTPTTESAIHAINMGHLFLTMPE
                     VSPDGTVTARGLINPLILWVWGGGAIMGLGVILNIFRPRRSEVE"
     gene            264..842
                     /locus_tag="VNV63_01910"
     CDS             264..842
                     /locus_tag="VNV63_01910"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HXC61406.1"
                     /translation="MNPEKQGFIRGWHITLVIGVFGLLALFYQGLWGDPRALPTVLIG
                     TPAPAFVGPELYSGEPISLAGFHGKVVVINFWASWCEECKLEHANLLKIYERFSKNPN
                     FVMLGIDYQDREDDAKEYLRIRGSNFKHIRDLKGTIAIDYGVYGVPETFVFDPQGIIR
                     HKQVGPILGPTYDKLTDQIIQPLLQGQPLQTS"
     gene            839..1312
                     /locus_tag="VNV63_01915"
     CDS             839..1312
                     /locus_tag="VNV63_01915"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HXC61407.1"
                     /translation="MKRFLIIAVLFMIAAPAVAAAGTVDEQTIDIRTREIAKTLRCTV
                     CQSESVFESQAPLAQQMRGVIRERVAQGQSDEEIRAYFLSRYGDYILMEPRRTGMNWL
                     IWAGPFLLLFIGGLFLYRTLARWAAQTASAPPEDLPPLDDISRQRIDRELRSLPK"
     gene            1328..>1867
                     /gene="ccmI"
                     /locus_tag="VNV63_01920"
     CDS             1328..>1867
                     /gene="ccmI"
                     /locus_tag="VNV63_01920"
                     /inference="COORDINATES: protein motif:HMM:TIGR03142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HXC61408.1"
                     /translation="MFAITIGLMLAIVILVLSYPFWSKDQTPMPVGYDTSEAQERIDL
                     DIEKEVILTSLAELDIDLAQGKLTQSDYQRLKATDEHRLLQILNKQDRTIAADRPSAT
                     RKETPLSQHAPIMHWTGSVLLGLLVIGSASGIYYYLDWKQTAELQAAQRQAGSGGQGM
                     PDPREMVTRLEARLRTNPND"
BASE COUNT          422 a          530 c          507 g          408 t
ORIGIN      
        1 gtcttccgcg ataacctatt ggtcaccgaa ctgcgtccgc agaaacggct ttacacggaa
       61 tcccagacac cgaccacgga atctgcgatc catgcgatca atatgggtca cctctttcta
      121 accatgccgg aggtttctcc ggatggcacg gtcacggcca ggggactgat caatccgctg
      181 attctctggg tctggggcgg aggcgccatt atgggactgg gcgtgatact gaatatcttc
      241 aggccccgca ggagcgaagt ggaatgaatc cggaaaaaca gggcttcatt cggggctggc
      301 atatcacact cgtgattggg gtcttcgggc ttttggccct cttctatcag ggattgtggg
      361 gcgatcctcg agcacttcca acggttctca tcggaacccc cgcgcctgct ttcgttgggc
      421 cggagctgta cagcggcgag ccgatctccc tggcgggttt tcatgggaag gtggtcgtca
      481 tcaacttttg ggcctcctgg tgcgaggagt gcaaactaga acacgcgaat cttctcaaga
      541 tctacgaacg ctttagcaaa aatccgaatt ttgtgatgct gggaatcgat tatcaggacc
      601 gggaggacga cgcaaaagag tatcttcgga tccgtggcag taattttaag catattcgcg
      661 acctgaaggg aaccatcgcg attgattatg gcgtctatgg tgtcccggaa acctttgtgt
      721 ttgacccgca ggggatcatc cggcataagc aggttggtcc gattctcggg cccacctatg
      781 acaaactaac ggatcagatt attcagcctc tcctgcaggg acagccgcta caaacctcat
      841 gaaaagattt ctgatcatcg ctgttttatt tatgatcgcc gctccggcgg ttgcggctgc
      901 cggaacggtc gacgagcaaa ctattgacat ccgaacccgg gagattgcaa agaccctccg
      961 ctgcaccgtt tgtcaatccg agtcggtctt tgagtcgcaa gcccccctgg ctcaacaaat
     1021 gcgcggggta atccgggaac gcgtagcgca gggacagtcg gatgaagaga tccgggccta
     1081 ttttttaagc cgctatggag actatatctt aatggaacct cgcaggacgg gcatgaactg
     1141 gctgatctgg gccggaccct ttctcctgtt gttcatcggc ggactctttc tgtaccgaac
     1201 ccttgcgcgc tgggcagcac agaccgcgtc tgctccgccg gaagacctcc cacccctcga
     1261 cgacatttcc cgtcagcgta tcgaccggga actgcgctcc ctcccaaaat aggaaagttc
     1321 ccgactcatg tttgccatca caatcggatt gatgttagcc atcgtcattc ttgtcctgtc
     1381 ttatcccttc tggagcaagg accaaactcc gatgccggta ggctatgaca cctccgaggc
     1441 tcaagaacgg attgacctgg acatagaaaa agaagtgatc ctgacctccc tcgctgaatt
     1501 agacatcgac ctggctcaag ggaagctgac ccagtcggac tatcaacgat taaaggcgac
     1561 cgatgagcac cgtctcctac aaattctgaa caagcaggat cgaacgatcg cggcagaccg
     1621 cccctccgca actcggaagg aaacgccgct gtctcagcac gccccgatca tgcattggac
     1681 cggttccgtt ctccttggcc tgctcgtcat cgggagcgca tcgggcatct attattacct
     1741 tgattggaaa caaacggcag agctgcaggc ggcacagaga caggccggct cgggcggaca
     1801 gggaatgccc gaccctcggg aaatggtgac gcgactcgag gcgcgccttc ggaccaaccc
     1861 aaacgac
//
LOCUS       DATMKH010000153         3093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_715561,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000153 DATMKH010000000
VERSION     DATMKH010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_715561"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..969
                     /gene="pyrF"
                     /locus_tag="VNV63_01925"
     CDS             187..969
                     /gene="pyrF"
                     /locus_tag="VNV63_01925"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001273.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HXC61409.1"
                     /translation="MSNQVACMSDTRKFKLSPKDRIIFALDFQSLKEATPLIRDLKDH
                     VGLFKVGLTLLLSEGIKVINQIQDISGDQKARIFLDLKLGKGLDIPNQMLGMLSVLIS
                     NSQGVEFITVHMTQGEKIGKEVVDKFKNGTKVLGVTVLTSTSKEESKRLYGTDVLDSV
                     IALSHTAKEAGCSGVVCSGHEVKAIKKLFGHGFITVTPGIRPSWSSIQKDDQRRVITP
                     REAIENGADYIVVGRPISLDKDPVGAADKISEEIAQALVQPE"
     gene            1054..1698
                     /locus_tag="VNV63_01930"
     CDS             1054..1698
                     /locus_tag="VNV63_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HXC61410.1"
                     /translation="MLHSWVVVWFGLVRDWGYPGIVLLMAMESSVFPVPSEIVIPPAA
                     YWAAQGRYNFWGVVLAGTIGSYVGSAVMYWVARWIGRPLVLRHGKYFFVSEAKLLRAE
                     RWLARYEAGGIFFARLLPVVRHLIGIPAGVVRMNFKLYSLMTVIGSALWCWVLAGFGS
                     KVIGDRPGLLNDPGQLMAVLTDRFHWFVGLALALVVLYIVVMRLTAKPASAVKQ"
     gene            1778..2797
                     /gene="ruvB"
                     /locus_tag="VNV63_01935"
     CDS             1778..2797
                     /gene="ruvB"
                     /locus_tag="VNV63_01935"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018046736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HXC61411.1"
                     /translation="MQERILTSELNEEEKRDEASLRPQTLAEYIGQTRIKENLKIYIE
                     AAKQRGDVLDHVIFYGPPGLGKTTLAYIIAREMGVNIKATSGPAIEHPGDLAALLSNL
                     SERDVFFIDEIHRLHPAIEEVLYPAMEDFQLDIIIGQGPSARSIKLALPRFTLIGATT
                     RAGLLTSPLRERFGVINRLDFYKLSEIEEIVIRSSRMLKIAVNASGATEIARRSRGTP
                     RIANRMLKRVRDFAQIKADGKISQEVACQALERMEVDEQGLDPMDRKLLLTIIEKFGG
                     GPVGIETVAAAINEEKETIEDVYEPFLIQSGYLDRTPRGRIATGLAYEHFKIARPKEG
                     QERLL"
BASE COUNT          764 a          763 c          840 g          726 t
ORIGIN      
        1 atcgcacacc agcggtgcgg cgaccacgac gtccacgact acctggcgca ggtcaacgcg
       61 atgaagcggc agttcggtcg gctggcggcc acagactttg accgttagcc cttgcggtta
      121 tgtgacaaca acgaactaca tacttgactt atattgtaat atgtcatacg attcggctca
      181 cgtcgcatgt caaatcaagt tgcctgtatg tcggacacca gaaaatttaa actctcacct
      241 aaagatagaa tcatttttgc tcttgatttc caatctctaa aagaagcaac cccgttaatc
      301 agggatctta aagatcatgt gggattattt aaagtcggtc ttactctgtt gcttagcgaa
      361 ggcataaaag ttataaacca aatccaggat atttctggcg atcagaaagc aagaatcttc
      421 cttgatctta aattgggcaa agggctcgac atacccaacc agatgttggg catgttatct
      481 gttttgattt ccaattcaca aggagtcgaa tttataactg ttcatatgac gcaaggagaa
      541 aaaattggga aagaggttgt ggataaattc aaaaacggga ctaaagtttt gggtgtcaca
      601 gttttgacga gcacatcaaa agaagaatct aaaagattat atggcacaga tgttttagac
      661 tctgtgatag cattatccca tactgctaaa gaggccggct gtagtggtgt ggtttgctca
      721 ggacatgagg ttaaagcgat aaaaaaactg tttggccatg gttttatcac tgtcactccg
      781 ggaatccgcc catcctggag cagcatacag aaagacgacc aacgcagagt gataactcca
      841 agagaggcca ttgagaacgg tgccgattac attgtagttg gaaggcctat ctctttagac
      901 aaagatccag tcggcgccgc agataaaatt tctgaagaaa tagctcaggc tttggtacag
      961 cccgaataat cccataaaat tttcatcaat cccatttccc acaattgagt tgtcatttgc
     1021 ttgtgcagtt tggttcctgt agaatcgggg gccatgttgc attcatgggt tgtcgtctgg
     1081 ttcggactgg tgcgggactg gggctatccc gggatcgttc ttctgatggc gatggagagc
     1141 tccgtcttcc cggttcccag cgaaattgtg attccgccgg cggcgtactg ggcggctcag
     1201 ggccggtata atttctgggg tgtcgtcctg gccggcacca tcggcagcta tgtcggctcg
     1261 gccgtgatgt actgggttgc ccgatggatc ggccgcccgt tggtcctccg gcacgggaaa
     1321 tatttcttcg tctccgaagc caagctcctc cgcgccgagc gatggctggc ccgctacgag
     1381 gcggggggaa tcttcttcgc gcggctcctt cccgtcgtgc gacatttaat cgggattccg
     1441 gccggagtcg tccggatgaa tttcaaactg tacagtctca tgaccgtgat cggatcggcg
     1501 ctctggtgct gggtcctggc ggggttcgga tccaaggtca ttggcgaccg tcccgggctg
     1561 ttaaatgatc ccggtcagtt gatggccgtc ttgacagacc ggtttcactg gttcgtgggg
     1621 ctggcgctgg cactcgtcgt cctgtatatt gttgtcatgc gcctgaccgc aaaaccagcg
     1681 tcagccgtaa aacagtaaag acgtttttat ccggataacc tctctccttg acatccctct
     1741 gatttctcgc taaagttagc cgaatcgaaa gggccgcatg caggaacgga ttctaacatc
     1801 agagctcaat gaggaagaaa aaagagacga ggcctcgctc cggccccaga cgttggcgga
     1861 gtatatcggc cagacacgga tcaaagagaa cctcaagatc tatattgaag cggcaaagca
     1921 gcggggcgat gtcctggatc atgtgatctt ctacggcccg ccgggtctgg gcaagaccac
     1981 actggcttac atcatcgcac gggaaatggg cgtgaatatc aaggccactt ccggcccggc
     2041 gatcgagcat cccggtgatc ttgcggcatt gttatcgaat ctttcagagc gggacgtatt
     2101 ttttattgat gaaatccacc ggctgcatcc ggccatcgaa gaggtgctct accctgcgat
     2161 ggaagatttc cagctcgaca tcatcattgg ccagggcccc tcggcccgga gtatcaagct
     2221 cgccttgccg aggtttacct tgatcggggc gactacgcgc gcgggtctgc ttacctcgcc
     2281 gctgcgcgag cgattcggcg tgatcaaccg gctcgatttt tataagctgt ccgagattga
     2341 agagatcgtg atccgctcct cccggatgct caagattgcg gtgaatgcct ccggcgccac
     2401 ggaaatcgcg cggcggtcgc gcggtacgcc acggatcgcg aatcggatgc tcaagcgggt
     2461 ccgggacttt gcgcagatca aagcggacgg caaaatcagc caggaagtgg cgtgtcaggc
     2521 cctggagcgg atggaagtgg atgagcaggg cctcgatccc atggatcgga agctgctcct
     2581 cacgatcatc gagaaattcg gcggcggacc ggtcgggatc gagaccgtgg ccgcggcgat
     2641 caacgaagag aaggaaacga tcgaggatgt gtacgagccg tttttaatcc aaagcggcta
     2701 tctggatcgc accccgcgag gccggatcgc caccggcctg gcctatgagc atttcaagat
     2761 tgcacggccg aaggagggtc aagagaggct tctctaaatt ttttgcgacg tgtttgtatc
     2821 ccaatctaaa aaaacagctc tccactttcc aaattccgaa ccatacaaaa cctgttgcaa
     2881 aataaaaaga aataggttag gatatttgtt cttttagacg agatatttgt tcatcatgac
     2941 cgagaagatt caacagtacc gaaaagaaat cgacaagctc gatgagaaga ttctcgacct
     3001 tctcaacaag cggaccgggt ttgtcatcaa gatcggcaag gtgaaaaaag aaaacaggct
     3061 ggaggcctac acgccgggcc gcgaacaaga gat
//
LOCUS       DATMKH010000154         1342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4242930, whole genome shotgun sequence.
ACCESSION   DATMKH010000154 DATMKH010000000
VERSION     DATMKH010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4242930"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1005)
                     /locus_tag="VNV63_01940"
     CDS             complement(<1..1005)
                     /locus_tag="VNV63_01940"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HXC61412.1"
                     /translation="MSDKISSEENERLQPFFTNLDRDIFGLKLPQEVAGALFSRYSRS
                     SKGLRQVFLDEFLGQEGLTLPALPAEHDSAAIKKAREFYDRVLIGYGDDSVAQLGGAH
                     IACENISNVAANLLEDSRIGMAPLEKSTRYVRFDKKDANGEYLIFKEPRIMASRHAGE
                     YLSVMNLLFETYAAQLDPMIEFVKKSLPISEMEFRHPKTGEPLNFGEIQKDEALTKWA
                     ETAYRSTVRSQACDILRGYLPAATKTNVGLFGTGQAFEYLLMKLYSNPLQEARDLAAA
                     IHRELDGLIPSFVKRARASAFLAETQAAIRKVSYQKLAGTSIEKTNAVTLIDYDAEAE
                     E"
     gene            complement(1097..>1342)
                     /locus_tag="VNV63_01945"
     CDS             complement(1097..>1342)
                     /locus_tag="VNV63_01945"
                     /inference="COORDINATES: protein motif:HMM:NF024820.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXC61413.1"
                     /translation="SIRINPDPWEAYYRLGYIYNEMGRYKEAVAMFEKVLERQPHFPE
                     IEKQFADLRKKLPAQSPIHKKNLKKPFKSLKIARSAR"
BASE COUNT          305 a          355 c          357 g          325 t
ORIGIN      
        1 ctcttccgcc tcggcatcat agtctatcaa cgtcacggca tttgttttct cgatggatgt
       61 cccggccaat ttttgatagg agaccttccg gatcgccgcc tgagtttctg caagaaacgc
      121 gctggcccgg gcgcgtttga caaatgacgg gatcaggccg tccagctctc gatgaatcgc
      181 ggccgccagg tcgcgggcct cctgaagcgg attcgaatag agcttcatga gcagatactc
      241 aaacgcctgc ccggtgccga aaagaccaac attggtcttg gtggctgccg gaaggtagcc
      301 gcggagaata tcacaggcct gggagcgaac tgtggaccgg taagcggtct cggcccattt
      361 cgtgagagct tcgtcttttt gaatctctcc aaagttcagc ggctcaccgg tcttcggatg
      421 tctaaactcc atctccgaga tcggcaggct ctttttgaca aactcgatca tgggatcgag
      481 ctgggccgca tacgtctcaa acagcagatt catcacgctg agatactcac ccgcatggcg
      541 ggaagccatg attcgcggct ctttaaagat caaatattcg ccgttcgcat cttttttatc
      601 aaacctgaca tagcgtgtgg atttttccag cggcgccatt ccgattcgtg agtcctcgag
      661 caggtttgcc gcgacattcg agatattctc gcaggcgatg tgggcgccgc ccagctgcgc
      721 caccgaatca tccccgtagc cgatcaggac acggtcatag aactcacgcg ccttcttgat
      781 cgcggcggaa tcgtgctcag caggcagtgc gggaagcgtg aggccttcct gacccaaaaa
      841 ttcatcgagg aaaacctgtc gcagcccctt tgaagaacgg ctgtagcgcg aaaaaagcgc
      901 cccggccacc tcctgcggaa gcttcagacc gaagatatcc cggtcgagat tggtaaaaaa
      961 cggctgtagc cgttcgtttt cttctgagga gattttatcc gacatgtttc ctctcaacga
     1021 ggattactat catacaaaat ccgggttgaa aggacaagtt aaaacgatgg aattgaggta
     1081 tccggggagg tgtcagctaa cgtgcagacc gggcgatctt caacgactta aaaggctttt
     1141 tgaggttttt tttatgtatt ggagattgag ccggaagttt tttacggagg tcggcaaact
     1201 gcttctcaat ttcagggaaa tggggctgtc gctccaggac cttttcgaac atcgccacag
     1261 cctctttata ccttcccatt tcgttgtaga tatagcccag gcgataatag gcttcccacg
     1321 ggtcgggatt gatccgaatg ga
//
LOCUS       DATMKH010000155         1936 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1039179, whole genome shotgun sequence.
ACCESSION   DATMKH010000155 DATMKH010000000
VERSION     DATMKH010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1039179"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1936)
                     /locus_tag="VNV63_01950"
     CDS             complement(<1..>1936)
                     /locus_tag="VNV63_01950"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXC61414.1"
                     /translation="SPATGEIYRYTLEGDQITVMDLRSLEDWVLEREFKKIPGIVDVV
                     SFGGPVKQYQVQVDPFRLKYFNITLQQLLNAVANSNNNIGANVLARGEQAYVVRGLGL
                     LQSIKDIENIVVASHGGTPTLVKDVATVTVGEQPRLGLVGKDHRDDVVEGIVLLRRGE
                     IPEEAIARIRERVPEINRSFLPPGVRIVPYYDRTNLIDTTTHTVLKNLTEGMILVIVV
                     LLLFLGNVRSALIVTLTIPLSLLSAFILMNLRGISANLLSLGAVDFGIIVNAAVILVE
                     NVFRHLSENGKREDAGSTILSSIQEVQGPIFISTAIIIAAYIPLFAMQGVESRIFTPM
                     AYTMGFALVASLILTLTLAPVLSDIFLTKGPLRERTLVMAPLRRFLLPLLHQAFAKRF
                     WVLLGALALFFLSIVLIPQIGNEFMPTLEEGNIWLRASLPMTIDLSYASRLVHQMRET
                     LLDFPEVKTVVSQLGRPDDGTDATGFFNAEYGVYLKPSSDWRSGMTKEKLVKEMEKKL
                     SAIPGVGYNFSQYIQDNVEEAISGVKGENVIKVFGPDLKVLESKANEIVPILHNIRGI
                     ENVGFFRELGQPELAINIDRERAARYGLNVADVETLVQVSIGGQAVTQVLEGERRYNL
                     VVRWLPEYRQDIDTITNILVD"
BASE COUNT          493 a          503 c          502 g          438 t
ORIGIN      
        1 tatcgactaa aatattggta atggtgtcga tatcctggcg gtactccgga agccatctca
       61 ccaccaggtt gtagcgcctt tctccttcga gaacctgtgt gacagcttga cccccgatag
      121 atacttgaac gagggtttcc acatctgcga cattcaaacc atatcgggcg gcacgctctc
      181 ggtctatatt aatggctagc tccggctggc cgagctcccg aaagaaaccg acattttcaa
      241 taccccggat attatgaagg atgggaacaa tttcattggc cttggactcc aggactttca
      301 aatccgggcc aaagaccttt atgacatttt cccccttgac ccccgaaata gcctcttcaa
      361 cgttatcttg aatatattgc gagaagttgt agccgacgcc cggaatagcg gaaagtttct
      421 tctccatctc cttcacaagc ttctccttgg tcattcctga gcgccagtcg gatgagggct
      481 taaggtagac cccgtattcg gcattgaaga atcccgtagc atcggtgccg tcatccgggc
      541 ggccaagctg cgagacgact gttttgactt ccgggaagtc aagaagagtt tcgcgcatct
      601 ggtggaccaa gcggctcgcg taagagagat caatggtcat tggaagactg gctctcagcc
      661 agatgtttcc ttcctccagc gtgggcatga actcattacc gatttgggga atgagaacaa
      721 tgcttaggaa aaagagggcc agtgctccga gtagaaccca gaatcgcttg gcgaaggcct
      781 ggtgcaggag aggcaggaga aagcggcgta gcggcgccat gaccaaggtg cgctcccgaa
      841 gagggccctt ggttaaaaaa atatcagaga gaaccggcgc caatgtcagt gtcaaaatca
      901 gagacgcgac caatgcaaac cccatcgtgt aggccatcgg tgtaaatatc cgcgactcca
      961 caccctgcat ggcaaacagt ggaatatagg cggcaatgat aatggctgtt gaaataaaga
     1021 tcggcccctg gacttcttga attgatgaaa ggatggtgga tccggcatct tctcgtttac
     1081 cattctcaga gaggtgccgg aagacgtttt ccaccagaat gactgctgcg ttgacgatga
     1141 ttccaaaatc gacggctccc aacgagagga ggttggccga tatccccctc aggttcatca
     1201 ggataaaggc ggaaagaaga gagagaggaa tcgtgagcgt aacgatcaaa gcgcttcgga
     1261 cgtttccaag aaaaaggaga aggacaacga tcacaaggat catgccctct gtgagatttt
     1321 tcaagacggt atgagtcgtg gtatcgatga gattggtccg atcgtagtaa ggaacgatac
     1381 gcacaccggg aggaagaaaa gatcgattaa tctcgggcac tctttcccga atccttgcaa
     1441 tcgcctcctc ggggatttcg ccccgacgca gaaggacaat tccctcgacc acatcatccc
     1501 ggtgatcctt cccaacaaga ccgagacggg gttgctcacc taccgtcact gttgccacat
     1561 ccttcaccag cgtgggggtc ccgccgtgcg aggcgacaac aatgttctcg atgtctttaa
     1621 ttgactggag cagtcctaaa ccccggacca cataggcctg ttctcctctg gcgagtacgt
     1681 tggccccaat gttgttgttg ctgttggcga cagcgttgag gagttgttga agagtgatgt
     1741 tgaagtactt caggcgaaag ggatccacct gcacctgata ctgcttcacc ggcccgccga
     1801 agctgaccac gtcgacaatt cccgggatct tcttaaattc ccgttcgagg acccaatcct
     1861 ccaaactccg gagatccata acggtaattt gatccccttc taatgtataa cggtagattt
     1921 cccccgtagc cggaga
//
LOCUS       DATMKH010000156         3344 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3283042, whole genome shotgun sequence.
ACCESSION   DATMKH010000156 DATMKH010000000
VERSION     DATMKH010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3344
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3283042"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="VNV63_01955"
     CDS             <1..496
                     /locus_tag="VNV63_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HXC61415.1"
                     /translation="RMASRILGMGDVLSLIEQAQEVFKENQAEELEKKIRSDSFTLED
                     FQEQLRQVKRLGSFDHLLGMIPGGERLKGMMINEQPEKEMKRAEAIVNSMTKMERRNS
                     AVINGSRRKRIAKGSGTSVENVNRLLKQFFEAKKMMKAFTGKRGRGGLPIGGLFKAFK
                     GFGG"
     gene            527..784
                     /gene="rpsP"
                     /locus_tag="VNV63_01960"
     CDS             527..784
                     /gene="rpsP"
                     /locus_tag="VNV63_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018750844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HXC61416.1"
                     /translation="MSVTIRLSRRGRHKRPFYRIVVADSRMPRDGRCIEVIGTYDPLA
                     KPAAVQVKKDRAEVWLAQGAQVSDTVGDLLHQIGLKRPAKN"
     gene            854..1084
                     /locus_tag="VNV63_01965"
     CDS             854..1084
                     /locus_tag="VNV63_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012625423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HXC61417.1"
                     /translation="MKELIEYIAKALVDKPEEVSVKETEGERTTILELRVAQEDLGKV
                     IGKEGRTARSMRVILNAAGTKLGKRCVLEILE"
     gene            1101..1916
                     /gene="trmD"
                     /locus_tag="VNV63_01970"
     CDS             1101..1916
                     /gene="trmD"
                     /locus_tag="VNV63_01970"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HXC61418.1"
                     /translation="MAESLTQPVSPDQSRQCLECDILTLFPLMVQPVLEQSILKKARD
                     LGLLSVKVYNLRDFTQDKHQTADDYPYGGGGGMIMKPEPIFRAVDEIQKTGRSIRFLL
                     TSPQGRPFDQEMAVELSRETKRLVIICGHYEGVDERVRIGLDLEEVSIGDFVLTGGEL
                     PALVVLDAAVRLIPGVLNDPSCSDQESFSQPFLDYPQFTRPVEYRGLRVPEILLSGNH
                     QAVARWRKQQAVLNTLKKRPDLAFDENLSVDERQMLAEARRELAESVLREQDS"
     gene            1969..2403
                     /gene="rplS"
                     /locus_tag="VNV63_01975"
     CDS             1969..2403
                     /gene="rplS"
                     /locus_tag="VNV63_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HXC61419.1"
                     /translation="MDRLERIDRGLEKKSIPLFKIGDTVKVHVKILEGEKERIQVFEG
                     VVIGRKGARNREMITVRKVSYGVGVERIFPLHAPTLDKIEVVREGRVRRAKLYYLRSL
                     KGRAARISEREFIQEAPGEKGAVADAEPKITEEVAKVPVTDS"
     gene            2459..3106
                     /locus_tag="VNV63_01980"
     CDS             2459..3106
                     /locus_tag="VNV63_01980"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014958702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HXC61420.1"
                     /translation="MPSRRGPGRVAQMTVFEEAAYAQGYRCIAGLDEVGRGTLAGPVV
                     AAAVLFRPETAITGVRDSKQLSPQQRDSFYPIILQEAVAVAVGMVEAAVIDQINILQA
                     TRLAMQRAIEQLTVQPDYLLLDAITLPLVPIPQRPIIKGDQLSISIAAASIVAKVTRD
                     RLMSRYHEEYPQYNFLAHKGYGTIEHRKQILEHGPCQIHRKTFRGVCDVDQLFPL"
     gene            3127..>3344
                     /locus_tag="VNV63_01985"
     CDS             3127..>3344
                     /locus_tag="VNV63_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007293543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HXC61421.1"
                     /translation="MSIAPRQFGIDGESLAAEFLRKKGYRILERNYRTQRGEMDIIAE
                     EGGALVFVEVKARRGDQFGGPHSAVGWR"
BASE COUNT          858 a          705 c         1071 g          710 t
ORIGIN      
        1 ccggatggct tcgaggatac tggggatggg tgatgtccta tctctcatcg agcaggccca
       61 ggaggttttt aaagaaaacc aggccgagga attggagaag aagatccgct cggacagctt
      121 taccttggaa gactttcagg aacagctccg ccaggtaaaa cggcttggtt cttttgacca
      181 tctgttgggg atgatcccgg gtggtgagcg attgaaggga atgatgatta atgagcagcc
      241 ggaaaaagag atgaagcggg cggaggccat tgtaaactcc atgacgaaga tggagcggag
      301 aaactcggcg gtcattaacg ggagccgtcg gaagcggatc gcaaagggaa gcgggaccag
      361 cgttgagaac gtcaacaggc tcttaaaaca gttcttcgag gccaagaaaa tgatgaaggc
      421 cttcacgggg aagcggggac ggggaggctt gccgatcgga ggcctcttca aagcgtttaa
      481 aggattcggt ggttgatcaa aaaaatgaga ctgaaaggag tgagtcgtgt cagtaaccat
      541 taggttgtca agacgtggca gacataagcg gcctttttat cggatagtgg tagcagattc
      601 aaggatgcct cgggacggga gatgtatcga ggtcatcggc acctacgacc cattggccaa
      661 accagctgcg gtccaggtca aaaaggaccg ggcggaagtt tggctggcgc aaggtgctca
      721 ggtctcggat acggtgggag atctcctgca tcaaataggt ttaaaaaggc cagcaaagaa
      781 ttaagaataa agagaggaag gaaacaatga aaacttcctg cggagctggt taattagatg
      841 ggaggtggcg ctgatgaagg agcttatcga gtacatcgcg aaggcactcg tggacaaacc
      901 tgaggaagtt tcggtcaagg aaactgaagg agagaggaca acgatccttg aattaagggt
      961 ggcccaggag gacctgggca aggtaatcgg caaggaaggg aggaccgcgc gctctatgcg
     1021 tgtaatcctc aatgccgcgg gcacgaagct tggaaaacgt tgcgtgcttg agatattaga
     1081 atgattaagg aaaagagaga atggccgagt cattaacgca gcctgtaagc ccggatcaga
     1141 gtagacagtg cttggagtgt gacatcctca cactgttccc gctgatggta cagccggtgt
     1201 tggagcagag tatactgaaa aaggcgaggg atctgggcct gctctctgtt aaggtttata
     1261 atctccggga ttttacgcaa gataaacatc agaccgcgga tgattatccc tatgggggcg
     1321 gaggcgggat gatcatgaag ccggagccga tctttcgcgc tgtggatgag attcaaaaga
     1381 ctggacggtc cattcggttt ctgttgacct ctccccaggg ccggcctttc gatcaggaga
     1441 tggcggttga gttgagccgg gagacaaagc ggttagtgat catctgtggt cattatgaag
     1501 gagtggatga acgggtgagg atcgggctgg accttgaaga ggtttcgatc ggcgactttg
     1561 tgttgacggg cggtgaattg cccgctctgg tggtcttgga cgctgcggtc cggttgatcc
     1621 ccggggtctt gaatgatccg tcctgttccg atcaggaatc attctctcaa ccgttcctgg
     1681 attatcctca gtttacccgt cctgtggaat atcgtgggtt gcgtgtgccg gaaatcttgc
     1741 tgtcgggtaa tcaccaggct gttgcacggt ggcgaaagca gcaggcggtg ctgaacacgc
     1801 taaaaaaacg gccggacctg gcgtttgatg agaacctgtc cgtggatgag cggcagatgt
     1861 tggcagaggc acgcagggag ttggcagaat ctgtcttaag agagcaggat tcttgattat
     1921 tgtagctcgt tatctgattc tacagaaaag agggaaggag tactagaaat ggatcgctta
     1981 gagcggatag atcggggact cgagaagaag agcatcccgt tgtttaagat tggggacacc
     2041 gtgaaagtcc atgttaagat tctggaggga gaaaaggaac gaatccaggt ctttgaagga
     2101 gtcgtgatcg gacggaaggg cgcaagaaat cgggagatga taacggtccg gaaggtgtct
     2161 tatggggttg gtgtcgaacg gatctttcca ctccacgcac ccacgttgga taagatcgaa
     2221 gtggtgcgcg agggacgggt acgccgtgca aaactgtact atctgcgtag cctaaaagga
     2281 agggctgcgc gtatctcgga gcgggaattt atacaggaag ctccgggaga aaaaggtgcc
     2341 gtggccgacg cggagccgaa gataacggaa gaagtcgcca aggtgcccgt aactgattcg
     2401 taaatgatga ggacaactgc ctgcccttaa cgggccccaa cgggatattt aaatggccat
     2461 gccgagtcgt aggggaccgg gtcgcgtcgc gcagatgacg gtgtttgaag aagccgccta
     2521 tgcgcagggt taccgatgta ttgcgggact ggatgaagtg ggccgaggga cactggcagg
     2581 accggtcgtg gccgcggcgg ttttattccg gccggaaact gccatcacgg gtgtaagaga
     2641 ttctaaacaa ctcagtcccc aacaaagaga cagcttttat ccgattatac tgcaagaggc
     2701 cgttgcagta gccgtgggca tggtggaagc ggcggtcatt gatcagatca atatccttca
     2761 ggcgacacgg ctggcgatgc aaagggccat cgagcagctc acggttcagc ccgattactt
     2821 gttgttggat gcgatcacct taccgctcgt cccaattcca cagcgcccta tcatcaaggg
     2881 agaccagctg agcatctcga tcgctgcggc ttcaattgtg gccaaggtga cgagagaccg
     2941 gttaatgtct agataccacg aagaatatcc gcaatacaat ttcttggcac ataagggtta
     3001 cgggacgatt gagcaccgaa aacagatcct agaacacggg ccctgccaga tccatcggaa
     3061 gacatttcgc ggggtgtgcg atgttgatca attgtttcct ctgtaaaaga cattaggtga
     3121 gtttccatgt ccattgcgcc gcggcagttc ggtattgacg gtgagagtct cgcagcggag
     3181 ttcttgagga aaaagggata tcgaatcctt gagcgtaact accgaaccca gcgaggcgag
     3241 atggatatca ttgcggagga aggcggggcc ttggtattcg tggaggtaaa ggcccggcgc
     3301 ggagaccaat ttggcgggcc ccacagtgcg gttggatgga gaaa
//
LOCUS       DATMKH010000157         2131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4243788, whole genome shotgun sequence.
ACCESSION   DATMKH010000157 DATMKH010000000
VERSION     DATMKH010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4243788"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..928
                     /locus_tag="VNV63_01990"
     CDS             <1..928
                     /locus_tag="VNV63_01990"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HXC61422.1"
                     /translation="SSEARSQVGAVWREELPQEAGATFLEMIERARRGEIKALYLIGE
                     NPIGTLPASMKVREALENLELLICQDLFLTQTGEIASVVLPAASYAEKEGTFTNHEGR
                     VQQVRQAFGPIDGSRPDGEIVTDLARLIGYPLEYDSPKAVFQEIQRLIPGYHVTALTA
                     ERRQAVAAALNRYLDQDFSKDLSQRYALTTPAANGNPYTLILGPMLFHSGKLSLKSEG
                     LLKLAQEGRLQINKEDAERLGLKDGDPARIASDQGRAEVKVKINSKLPAGLVFFPEHF
                     NQPSIKDLIPVSVDPENRVPSYRTASVTVERI"
     gene            928..1977
                     /gene="nuoH"
                     /locus_tag="VNV63_01995"
     CDS             928..1977
                     /gene="nuoH"
                     /locus_tag="VNV63_01995"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013450449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HXC61423.1"
                     /translation="MELAIKIGVTMVEIAAAMAIVLFHVAYLTYAERKILGFMQDRLG
                     PMEVGFHGILQPIADGLKLFFKEDIIPTGANKIIFSVAPILTLVPALIGFAVIPFGDE
                     GYSFFGVKISPYITDINIGILYILAISSVGAYGILLGGWASNSKYSLLGGMRSAAQMI
                     SYELALGLSIVGVILMSGSLSMVKITESQIGGFWHWSVLKFPAPQFIAFGIYVISAIA
                     ETNRLPFDLPEAESELVAGFMTEYSGMRFAFYFMAEYANMILVSAIAVTLFLGGWYAP
                     FPFLEFTWPSPWLWHFFWFAGKVYFILFFFIWIRGTLPRLRYDQLMKFGWKIMLPLAL
                     ANILITATLLYLVNG"
BASE COUNT          452 a          526 c          609 g          544 t
ORIGIN      
        1 ttcttccgag gcgcgttcac aagtgggcgc ggtctggcga gaagagcttc ctcaggaagc
       61 gggtgcgacc tttttggaga tgatcgagcg ggcgcggcgg ggagagatca aagcgctcta
      121 tcttatcggt gagaatccga tcggcacact tccggcctcg atgaaggtac gtgaggcctt
      181 ggaaaatctg gagctgctta tctgccagga cctttttctc acgcagaccg gtgagatcgc
      241 atctgtcgtc ctgcccgcgg caagctatgc cgaaaaggaa gggaccttta cgaatcatga
      301 aggccgtgtg cagcaggtcc gtcaggcctt tggcccgatc gacggatctc gaccggatgg
      361 tgagatcgtc acggatctgg cccggctgat cggctacccg ctggagtacg acagcccgaa
      421 ggcggttttt caggagattc aacggctgat tccgggctat catgtcaccg ctttgacggc
      481 cgaacgccgt caggcggttg cggcggcctt aaaccggtat cttgaccagg acttttcaaa
      541 agatctttct caacgctatg ctttgacaac gccggcagcc aacggtaatc cgtatacttt
      601 gatactgggc ccgatgctgt tccattccgg aaagctgtct ttgaagagtg aggggctgct
      661 caaacttgcg caggagggcc ggctacagat taataaggaa gatgccgagc ggctgggact
      721 gaaagatggt gatccggcgc ggatcgcatc cgaccagggt cgggcggagg taaaggtcaa
      781 gatcaattcc aaactgccgg ccggtcttgt tttcttcccg gagcatttta accaaccgtc
      841 aatcaaagac ctcattccag tctcggtaga cccggaaaac cgtgtgccgt cctaccgaac
      901 ggcatcggtc acggtagaaa ggatctgatg gaacttgcga tcaagattgg agtgacgatg
      961 gtggagatcg cggcagcaat ggctattgtt ctgtttcatg tggcctatct cacgtatgct
     1021 gaacggaaga tcctgggttt catgcaggat cggttgggtc cgatggaagt agggttccac
     1081 gggatcctcc agccgattgc cgatggcttg aagctcttct tcaaggaaga catcatccca
     1141 acaggcgcga ataaaatcat cttcagcgtt gccccgattc tcacgctcgt gcccgccttg
     1201 atcggttttg cggtgatacc ttttggagac gaaggctaca gcttcttcgg cgtgaagatt
     1261 tcgccttaca tcacggacat caacatcggg attttgtaca tactggccat ttcgtccgtg
     1321 ggagcctatg ggatactgct gggcggctgg gcgtctaaca gtaaatattc tctgctgggt
     1381 ggaatgcggt cggcggccca aatgatcagt tatgaactgg ccctgggact gtcgatcgtg
     1441 ggcgtgatac tgatgtccgg ttccttgagc atggtcaaga tcaccgagtc ccagatcggc
     1501 ggtttttggc actggtccgt acttaagttc ccggcaccgc aattcattgc cttcgggatt
     1561 tatgtcatct cggcgattgc ggagaccaac cggctgccgt tcgatcttcc ggaggccgag
     1621 agcgagctcg tggcaggttt tatgactgag tacagcggga tgaggtttgc tttttatttc
     1681 atggcggaat atgccaatat gatcttggtc tcggcaattg cggtaacgct gttcctgggc
     1741 ggatggtatg ctcctttccc gttcctggaa tttacctggc cttcgccctg gctctggcat
     1801 tttttctggt ttgcgggaaa ggtctatttt atcctgtttt tctttatctg gattcgtgga
     1861 acgctgccga ggctgagata cgatcaattg atgaagttcg gttggaagat catgctgccc
     1921 cttgccctgg ctaacatcct gatcacggcc actctgctct atctggtcaa tggttaattt
     1981 atcaggaata agcagatgaa gaagatcaaa aaaatcctga acgatctttt ctttttggag
     2041 atcctaaaag ggcttgtcct gaccttcaag catctctttg tcggaacagt tacgatccag
     2101 tatccaagag agaagcggaa acttccggtt g
//
LOCUS       DATMKH010000158         4248 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4555612, whole genome shotgun sequence.
ACCESSION   DATMKH010000158 DATMKH010000000
VERSION     DATMKH010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4248
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4555612"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..920)
                     /locus_tag="VNV63_02000"
     CDS             complement(<1..920)
                     /locus_tag="VNV63_02000"
                     /inference="COORDINATES: protein motif:HMM:NF021619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PocR ligand-binding domain-containing protein"
                     /protein_id="HXC61424.1"
                     /translation="MTPQPLDQGKKLGHLLQLTNWQNIQEAFSAMTGLSVLTYDTQGN
                     LYYEPSQENPICQTVQLTAKGQQHCKDHCGKNALLAATSQEPIFFKCQANLHVFSFPV
                     AVGDQFKLVLLGGKTYFNPQEFEDLRNHPDRVGTEEQRLIPLAKGIRLKEPLFLQSAA
                     PLLQKSAIAILEGIHYRQKFRTRLSQLITLFTIFAELRKELALSELHTLILHCVGVLF
                     DVSTATIFVREQDSKPFRAAQTFGRHKELLRTYQAEISEELLEPLQIAGRSVFTDSAY
                     ELLKSGFPRELRTAHLFSLSSRFSGPQTLLA"
     gene            1061..3055
                     /gene="uvrB"
                     /locus_tag="VNV63_02005"
     CDS             1061..3055
                     /gene="uvrB"
                     /locus_tag="VNV63_02005"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HXC61425.1"
                     /translation="MSRFKLISDFKPEGDQGQAIDTLANGVLQGQKHQALLGVTGSGK
                     TFTMANVIERVQKPTLVIVHNKTLAAQLYHEFKTFFPENAVAYFVSYYDYYQPEAYLP
                     QTDTYIEKDSSINDAIDRMRHAATTALFQRNDVIIVASVSCIYGLGSPEAYHGMLVYL
                     EEGKEVDRDAVLRKLVEIQYQRNDYDFHRGTFRVRGDVLEVFPASSEDKSIRIEFFGD
                     AVEAISEVDPLRGTMLKRLEKIAIYPGSHYVIPPDRMERALNGIEQELIERIKLFKQE
                     GRLIEAQRIEQRTYFDLEMIREVGYCHGIENYSRHLSGRLPGEPPPTLLDYFPKDYLL
                     IVDESHATTPQLHGMYEGDRSRKGNLVEYGFRLPSAFDNRPLKFHEFEQLVNQIVYVS
                     ATPGPYELEKAQGRVVQQIIRPTGLMDPKITIQPAKGQVDHLLGEIKKRTASGERVLV
                     TTLTKRMAEDLAEYYRELGIKVRYLHSEIKTLERTEIIRDLRLGLFDVLIGINLLREG
                     LDLPEVSLVAILDADKEGFLRSYRSLIQTAGRAARNVHGEVVMYADTITDSIQQTIQE
                     TNRRRVIQSAYNETHGIIPESIQKGIPPALYEISEADYYTVPIAAERTEEYLPKGELS
                     AIIKDMEKEMRAAAKNLEFERAAGLRDKLKELKELDLGVR"
     gene            complement(3084..3347)
                     /locus_tag="VNV63_02010"
     CDS             complement(3084..3347)
                     /locus_tag="VNV63_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009371109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4321 domain-containing protein"
                     /protein_id="HXC61426.1"
                     /translation="MLKRTPWMLIFFVLIGGLMGGVLGEILRLFSPEGLLKEIFLKGY
                     SIGINPPLTVDLHLFTFTLGFTIMMNLFSLLGIIFGIYVYKQA"
     gene            3443..>4248
                     /locus_tag="VNV63_02015"
     CDS             3443..>4248
                     /locus_tag="VNV63_02015"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HXC61427.1"
                     /translation="MKFLEDRSNVLKFLILIFLLVLAFGFIHLTDLGRYLIPGRIREM
                     ILSFGPWAPAIYLLLFSIGPVFMWPEAVLAMAAGLAFGPFWGTVLTILGATMGGTLAF
                     FVARYLGRDFVKRFFRSRLKMLDDKSAEHGFKIIFSLRLIPVVPFNALDYAAGLSKIR
                     FGDYVLGTFLGIMPGAFAYVNLGSNLENIYSWKFVLAVTFLGSLVWVQVFYQRWKRGR
                     PIGKLVVLIFFILAVILFFYFDLERYLTLAELKEHREALLVYTAAHYGPAV"
BASE COUNT         1002 a         1014 c         1214 g         1018 t
ORIGIN      
        1 gcaaggagcg tctgcggtcc tgaaaaacgc gaagaaagcg agaagaggtg tgcggtccgc
       61 agctctcgcg ggaatcctga tttcaaaagc tcataggcgc tgtccgtgaa gacagatcgg
      121 ccggcaattt gaagcggctc caacagctct tcggagatct cggcctgata ggtacggagg
      181 agttctttgt gtcgtccaaa ggtctgggcc gcccgaaagg gcttgctgtc ctgttctcgc
      241 acgaaaatgg tggcggttga gacgtcaaag aggacgccga cacaatggag aatgagcgtg
      301 tggagttccg aaagcgcaag ctctttgcgc aactctgcaa aaatggtaaa gagggttatg
      361 agttgagaga gccgtgtgcg gaatttttga cgatagtgaa ttccttccag gattgcgatg
      421 gccgactttt gaagcagtgg ggccgctgat tgcaggaaaa gcggctcctt gagtcgtatc
      481 cccttggcca aggggatcag gcgctgttcc tcggttccta cccggtcagg atggttacga
      541 agatcttcaa actcctgagg gttaaagtaa gtcttccctc ctaaaaggac cagcttgaat
      601 tgatcaccca cagccactgg aaaagaaaag acatgaaggt tggcctgaca tttaaagaag
      661 atcggctctt ggctggttgc agccaagagg gcattcttgc cacagtgatc tttacagtgc
      721 tgctgtccct tggcggtcaa ctgcacggtt tgacagatgg gattttcctg gctgggttcg
      781 taatagaggt ttccttgggt atcataggtc aggaccgaca agccggtcat ggcggagaat
      841 gcctcttgga tattctgcca gttagtcagc tggagaagat gccctaactt tttgccctgg
      901 tctaagggtt gcggagtcat gttttatgct ctgagatcgt taaatctata ttcattttat
      961 acaatagctt ggaagataag caaagtcaac tacactaaag tggcttcttt tctctgaaac
     1021 tgggatagaa aggtccgaaa gagttgtgtt atgctatgtc atgagccgat ttaaactgat
     1081 ttctgatttt aagccggagg gagatcaagg tcaagccatt gataccttgg ccaacggcgt
     1141 cctccagggt caaaaacatc aggccttgct gggagtgacc ggttcaggca aaacctttac
     1201 aatggccaat gtgattgagc gggtccagaa accgaccctc gttattgtcc acaacaagac
     1261 cctggcagcc cagctctacc atgagtttaa aacattcttt ccggaaaacg cggtggccta
     1321 ctttgtcagc tattatgatt actaccagcc ggaggcttac cttccgcaga ccgacactta
     1381 cattgagaaa gactcctcta ttaacgacgc gattgaccgg atgcgtcacg ccgcgactac
     1441 cgcgctgttt cagcgaaacg acgtgatcat cgtggcttcg gtctcctgca tttacggttt
     1501 aggttcgccg gaggcctatc acgggatgct ggtctatctg gaagagggaa aggaggtgga
     1561 ccgggacgcg gttttaagga agcttgtcga aatccagtac caacggaacg attatgattt
     1621 tcaccgagga actttcaggg tccgagggga tgttcttgag gtgtttccag cctcctcgga
     1681 agacaagagc atccggatcg aattttttgg ggatgccgtg gaggccattt ccgaggtaga
     1741 tccgttgcgg ggaacgatgc ttaaacggtt agaaaagatc gcgatctatc ctggaagcca
     1801 ctacgtgatc ccacccgatc ggatggagcg ggctctgaac gggatcgagc aggagttaat
     1861 cgagcggata aagcttttta aacaggaagg ccggttgatc gaggcgcagc ggatcgagca
     1921 gcggacatat ttcgatcttg agatgatccg cgaggtgggt tactgtcacg ggatcgagaa
     1981 ctactcgcgt cacctctccg gccgactgcc cggagagcct cccccgaccc tcttagacta
     2041 cttcccgaaa gattatctct tgatcgttga tgagagtcac gccacgaccc ctcagctcca
     2101 tgggatgtac gagggagatc gttcccgcaa gggcaatctc gtcgagtacg gcttccggct
     2161 tccttccgcc tttgacaatc gcccgctcaa gtttcatgag ttcgagcagc tggtgaatca
     2221 aatagtctat gtctcagcga cgcccgggcc ttatgagtta gagaaagccc agggccgcgt
     2281 cgtgcaacag atcatccggc cgaccggcct catggatccc aaaatcacga tccagccggc
     2341 gaagggacag gtagatcatc tcctgggcga gatcaagaag cgtaccgcga gcggtgagcg
     2401 ggtcctggtg accacactga cgaagcgtat ggcggaagac ctggccgagt attacagaga
     2461 actggggatt aaggtccgtt atctgcattc cgagattaaa acactcgagc ggaccgagat
     2521 catccgggat ttacggctcg gtctgttcga tgttttaatc ggtatcaatc tcctccgaga
     2581 ggggctggat ctgccggagg tatccttggt ggccatcctg gatgcggaca aggagggatt
     2641 cctgcggtct taccggtctc tcatccagac cgctggacgg gcggcgcgga acgtacacgg
     2701 tgaggtagtg atgtatgccg acacgatcac cgattcgatt cagcagacaa tccaagagac
     2761 caatcgccgc cgagtgatcc agagtgccta caatgagacc cacgggatca taccggaaag
     2821 catacagaag gggatacctc ctgcgctata tgagatctcg gaggcggact attacactgt
     2881 tccgatcgcg gccgagcgga ccgaggaata tttgcccaag ggggaactca gcgcgattat
     2941 caaagacatg gagaaagaga tgcgggcggc cgccaagaat ctggagtttg agcgagcagc
     3001 cggactacgg gataagctca aggagctcaa ggagttagat ttgggagtga ggtaggggga
     3061 cgatgcaacc cgccctgcag agtttacgcc tgtttgtaaa cgtagatccc gaagatgatg
     3121 ccgagaaggc taaacaggtt catcataatg gtgaagccga gggtgaaggt aaacaggtgg
     3181 aggtctactg tcagtggagg attaatcccg atactgtacc ccttgaggaa aatctcctta
     3241 aggagacctt cgggagaaaa cagccggagg atttctccca ggacgcctcc catgagcccg
     3301 ccaatcagga caaagaaaat caacatccaa ggtgttctct tgagcatggc catttaaagt
     3361 tagtcggctc caattggacg acagcctaat cattttgcaa cggcttgtca agctaaaatc
     3421 gggaacgcct cggccaattc tgatgaagtt cttagaagat cggtcaaacg tcctcaagtt
     3481 cctaatactg atcttcctgc tggtattggc gtttggtttc atccatttaa cagatctcgg
     3541 ccgttacctc ataccgggga ggatcaggga gatgatcctg tcatttggtc cctgggcgcc
     3601 cgcgatttat cttctcctct tcagtatcgg accggttttc atgtggccgg aagcggttct
     3661 tgcgatggcg gctggattgg cctttgggcc gttctgggga acggtgctga cgattctcgg
     3721 cgcaacgatg ggaggcacgc tcgctttttt cgtggcccgt tatcttggcc gagacttcgt
     3781 gaaacggttt ttccgaagcc ggctgaagat gctggatgat aaatcggccg agcatggatt
     3841 caagataatt ttttcccttc ggctgattcc ggtcgtgccc ttcaatgccc tggattatgc
     3901 ggccggattg tccaagatca ggtttgggga ttatgtcctg ggaacttttt tagggatcat
     3961 gccgggtgcc ttcgcatatg tcaacctggg gagtaatctg gagaatatct attcctggaa
     4021 atttgttctg gcggtgacgt ttctgggatc cttggtatgg gtccaggtgt tctatcaaag
     4081 gtggaagaga ggaagaccga tcggcaagct cgtggtcctg atattcttta tcctggccgt
     4141 gatccttttc ttttattttg atctggagcg ctatcttacc ctggccgaat taaaggaaca
     4201 ccgggaggcg ctgcttgttt ataccgcggc ccattacggg cctgccgt
//
LOCUS       DATMKH010000159         1105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_397902,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000159 DATMKH010000000
VERSION     DATMKH010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397902"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /locus_tag="VNV63_02020"
     CDS             complement(<1..689)
                     /locus_tag="VNV63_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61428.1"
                     /translation="MGKKDKDKKKKKWEEEEEEKQDQPGKRSAGTYADGHPLDQIQYL
                     EAKLILKPDRFTSVESFREFGKLVKKTAKKEDVGFVPDVEAGLRPNIREILFLDTPDF
                     RFYNNAFILRRRIAYVDGFPVGDPEIVFKYRHPDEKAAAAVDVRPKISGKYRIKFKAE
                     ALPLKDRIGSYRVLYSHNCVFGLSQVHEGDRTSMQTLVRVFPALEKLKKSPDERVTLV
                     SEAIVEEVLLK"
BASE COUNT          238 a          308 c          261 g          298 t
ORIGIN      
        1 aatttgagca gcacctcttc gacgatggct tcactcacca gggtcactct ttcgtcaggc
       61 gatttcttca gcttttcgag ggccgggaag acgcgcacca gcgtctgcat cgaggtgcgg
      121 tccccctcgt gcacttggct caacccgaac acacagttgt gagaatagag aactcggtag
      181 gagccaatac gatccttcaa tggcagcgcc tcggctttaa atttaatccg atattttcca
      241 gatatctttg ggcgaacatc taccgccgct gctgccttct catccggatg acgatatttg
      301 aaaacgatct cagggtcgcc cacaggaaaa ccatccacat aagcgatccg tcggcgaaga
      361 atgaaggcgt tgttgtagaa acggaagtcc ggggtatcca ggaatagaat ctcccgaata
      421 ttaggccgca aaccggcctc cacatccgga acgaatccga cgtcttcttt ttttgcagtc
      481 ttcttaacca gcttcccaaa ttcccggaaa ctttccacag atgtaaagcg gtccggcttc
      541 aaaatcagtt ttgcttcgag atattgtatc tgatcgagag gatgaccatc ggcataggtg
      601 cccgcggacc ttttacctgg ctgatcctgt ttttcctctt cttcctcttc ccacttcttt
      661 ttcttcttat ctttgtcctt tttccccatg atcgctcctc gtttagtgtc ttgtgcgtga
      721 caactggaga tcgcgagtcg gctcccgtgc ccttgccggt gatcgtgaac atcagctttt
      781 tcgggttgcg cctcagttct cgacgggagc gtcaaatggt tgcgcgactg agcgaacaaa
      841 tgtaactgtc aaaagtaaca gtcccgtcgg gctctttcac cgactgcttt tcttaggtga
      901 cgcttcgcgg tggccgcctg tctcttacta agagggacaa atcagcaaga cgccccccac
      961 cattaatctc agtctgagat aggtggtgat aatccctgtt ctcgacactt atctgagaga
     1021 aggcgtaaga tgcgcgcaag gtgctgtttc ctcgtccatt ctcggccgca cggatttgtg
     1081 cctaccgcat catcgaaatg ctcgg
//
LOCUS       DATMKH010000160         1417 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1040167, whole genome shotgun sequence.
ACCESSION   DATMKH010000160 DATMKH010000000
VERSION     DATMKH010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1040167"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..1253)
                     /locus_tag="VNV63_02025"
     CDS             complement(144..1253)
                     /locus_tag="VNV63_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61429.1"
                     /translation="MTSYTPPDGKHPYLSIVVSSRNDDHGGNILGRMRLFQKGLLEQS
                     RRYRFPIEVVFVEWNPPADRPRLHEVLPKPAEDDCLLLRYISVPNEIHRRYRRAPDID
                     LFQMTAKNVGIRRARGAFILCTNIDLLFSDALFCALAERSFKPDTYYRANRCDVPDGI
                     DPRWDLAQQLAWCERNVIRRLGRDRRYRNINLELLGQQDRGRVLKWFLDKMAHGVGLY
                     WNPGKELFYQLDLFACGDFTLMSREAWNAIQGYLELDLYSLHIDSLGLIAAAALGYKQ
                     HVFPLQACTYHIDHTKGWETLAPLDKVRFLADRPALDYSLIQEVALHAFEKREPFNLN
                     PPTWGFSDVELEELVFPPPSANDTRTLARSASAGD"
BASE COUNT          363 a          368 c          369 g          317 t
ORIGIN      
        1 cgttcgaaag atcttttcac ggatctgcct gacgtgttca gcgtttttgt cgatgccgag
       61 cacaccatag cccgctttct cgagattcaa agccaggcaa agcccgattc ttccgatccc
      121 gataaccaca attctttcaa acatcaatca ccagccgaag cacttcgggc cagagtgcga
      181 gtgtcattcg ccgaaggtgg aggaaataca agttcttcca attccacatc ggagaaaccc
      241 caggtgggcg gattcagatt gaatggctcc cttttttcga acgcatgcag cgctacttcc
      301 tggatcaggc tgtaatcgag cgctggacgg tcggccaaga acctgacttt gtcaaggggc
      361 gccaaagtct cccatccctt tgtgtggtcg atgtgatagg tacatgcctg cagagggaag
      421 acatgctgtt tgtaaccgag cgcagctgcg gcgatcaatc caagggaatc gatatgcaga
      481 gagtagagat caagctcgag atatccctgt atcgcattcc aagcttctcg ggacattaac
      541 gtaaaatctc cgcaagcgaa aagatccaat tggtaaaaca actcctttcc cggattccag
      601 tagaggccga cgccgtgagc catcttgtcg agaaaccact ttaggaccct tcccctgtcc
      661 tgctgcccga ggagctccag gttgatattc ctataacggc gatccctgcc cagcctacga
      721 attacatttc gttcgcacca ggccagttgt tgagccaaat cccatcgcgg atcgatgccg
      781 tcgggaacat cgcatcgatt ggcccgatag taggtatcag gtttgaacga tctctcagcc
      841 agggcgcaaa agagcgcatc cgaaaaaagc aggtcgatat tcgtgcacaa aatgaatgcc
      901 cctcgtgccc ggcggatgcc cacgttcttg gcggtcatct gaaataagtc aatatcggga
      961 gcgcgtcgat aacgacgatg aatctcgttt ggcacggaaa tataccggag taacaggcaa
     1021 tcgtcctcgg ctggtttcgg cagcacttcg tgcaagcgcg ggcggtccgc cggcggattc
     1081 cactcaacga aaactacttc aattgggaaa cgatagcgtc gcgattgttc caggagtccc
     1141 ttttgaaaga gtcgcatccg tcccaggata ttccctccat gatcgtcatt tcgtgaggag
     1201 acaacaatgg ataggtaagg atgcttcccg tcaggagggg tgtagcttgt catggcgcga
     1261 gtgattatag tatccgaaag gccaagttgc gacagaacaa atgccatgga cgaaatcagg
     1321 aacaagaacc tgataaagca gcttgatgcg gcggagaacg accgtgagtt ccttgttttc
     1381 ggtcagccgc aaatactcga ggatgagatt gaagaag
//
LOCUS       DATMKH010000161         1390 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2000124, whole genome shotgun sequence.
ACCESSION   DATMKH010000161 DATMKH010000000
VERSION     DATMKH010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2000124"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="VNV63_02030"
     CDS             <1..242
                     /locus_tag="VNV63_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61430.1"
                     /translation="EHHSFHGFKHMVVPVFGLLANLLCMLFYLIGPFSVAGMSWKEPF
                     IALGIAGVWGLYGAFHFTWGNKKKEKSLLVQQVPA"
     gene            649..>1390
                     /locus_tag="VNV63_02035"
     CDS             649..>1390
                     /locus_tag="VNV63_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61431.1"
                     /translation="MLVCEGELLDGKSPASLVWSNVGGRIRIKADKILGILEGRTRIK
                     AAVLQELYPGLFEKAPNPGTEFNIPLQTIVMQLEEIFTGISAEDSALEDFDTPFGQLA
                     RQDEARFKDQQADRSEMRPMAARTLFMLPQLGAKPVLHEQDQKASMGKLTENGSPKEV
                     VEESASRRGNSGPVELQSKEEKSAPSESSAIRESFERENGESGNSDGSGGIPQDNENI
                     PSSAGEPEPAIGKKFSAPASHSGASGVFA"
BASE COUNT          366 a          331 c          387 g          306 t
ORIGIN      
        1 gcgaacatca ctcgttccac ggattcaaac acatggtggt gccggtattc ggcctactcg
       61 cgaatctgtt gtgcatgttg ttttatctca ttgggccttt ctcggtggcg ggcatgagct
      121 ggaaagaacc gtttatcgcg ctcggaattg ccggtgtctg gggtctctac ggcgctttcc
      181 acttcacttg gggcaataaa aagaaggaaa aatcgctcct cgtgcagcag gtacctgcct
      241 agcgctcgaa aatctgttac agactcgggt ggaaagcagc gcgcagattc gaaacgatct
      301 gcgcgttgct gtgtctacag ggtacccgcc tttcatccgc tctgagaaaa ttctgacgga
      361 ggcgaaagag aaaacatcgc accgagatcc gcacggtgta acggaatgtt gcgctgcaat
      421 gacacgcagc atccgggaat tcctttttca ataaagccag cagcccgata ataacttatc
      481 taataaaacg taatacttta atgaaacaga ttgtgtttgg acaaaaatct tgcttagact
      541 tccgctttta tcgaaatgga tttccaaaga ctgttcaaac gaaaagaaga aggtcccgct
      601 cctcagatct tcagggataa gataagggaa acgttaaacg aaagggatgt gctggtctgt
      661 gaaggggaat tgttggacgg caaatcaccg gcgtcccttg tttggagcaa tgtaggcggg
      721 agaatccgca tcaaagctga taagatccta ggcatactgg aaggtcgaac ccggatcaag
      781 gcagcggttc ttcaggagct ttatcctggt ctatttgaaa aagctccaaa tcctggcact
      841 gaatttaata taccactcca gacgattgtt atgcagctcg aggagatatt caccggaatc
      901 tctgcggagg attcggccct cgaagatttc gacacccctt ttgggcagct cgctcgccag
      961 gatgaagcga gatttaagga ccagcaagcg gatcggtcgg agatgcggcc aatggcggcg
     1021 cgaacgttat tcatgctgcc acagctcgga gcgaaaccgg ttttgcacga gcaagatcag
     1081 aaggccagca tgggaaagct gacagaaaat ggttccccga aagaggtagt ggaagagagt
     1141 gcttcccgcc gtggaaacag cggtcctgtc gaactgcagt cgaaagagga gaagtcggca
     1201 ccttccgagt cgagtgcgat ccgggagtcc ttcgagaggg aaaatggaga aagcggcaac
     1261 tccgacggat ccggtggaat tccccaggac aatgagaata ttccttcttc cgcgggggaa
     1321 ccggagccgg cgatcgggaa aaaattctcc gcgcctgcat cgcacagtgg agcctcggga
     1381 gtcttcgctc
//
LOCUS       DATMKH010000162         1099 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4245150, whole genome shotgun sequence.
ACCESSION   DATMKH010000162 DATMKH010000000
VERSION     DATMKH010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4245150"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="VNV63_02040"
     CDS             complement(<1..302)
                     /locus_tag="VNV63_02040"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HXC61432.1"
                     /translation="MEVTHLIQIVIVLIASMVGLTILADRLAIPAPLILVPAGALLGF
                     MPWFPRIELNPELILLVFLPPLVYAGGAFSSWQTFQKNLRPIILLSVGLALFTMVVV"
     gene            complement(750..>1099)
                     /locus_tag="VNV63_02045"
     CDS             complement(750..>1099)
                     /locus_tag="VNV63_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HXC61433.1"
                     /translation="GVGMVTGRRQEENAGILTIEAPKEILRYCIKKGSVAVDGVSLTI
                     NDLNDKQFTVSIIPHTAKNTTLGIKGVGALVNLENDPIAKYVERLLQTEEPFSVRPEI
                     KIDIGFLKKHGYV"
BASE COUNT          280 a          282 c          268 g          269 t
ORIGIN      
        1 acgacgacca tcgtgaagag agccagtccg accgacaaaa gtatgatcgg ccggagattt
       61 ttctggaagg tctgccagga cgagaacgct ccgccggcat agacaagcgg cggcaggaag
      121 acaagaagta tcagctccgg attgagctcg attcgcggga accacggcat gaaaccgagt
      181 aaggctccgg ccggaaccaa aatcaacggc gcagggatcg ccaggcgatc ggcgaggatc
      241 gtcaagccga ccatcgaggc gatcaatacg ataacgatct ggatcaggtg ggtgacttcc
      301 atgagcgctg ttttgtgata gccggcccca gtataggccg aacgtaaaaa aacatcaagg
      361 cgtcaagcgg atggtttacg gggcagtgat caacgcttcc cgcgattcct gatgattgaa
      421 acaaccctaa aaagaaaata gagagcaacg gccgcgtcca ttacaataaa taaaacgaat
      481 agaatcttat ttaggttcat tcctgatatt gtcccggttt tacttattag gagcagaaat
      541 catacggtta tagcacttat cacacattcc accaactctg ttcccggtta aaacacttcc
      601 gcatccgttg cattttccag catacgaacc gcctatcttc tttttctcgg aaacgctttt
      661 acttttcttc atgatctctc ctcattcggg ttaagtcaga taaagaccaa accttatctg
      721 taattcctgt tacggagaag ttaaaggggt tatacgtacc cgtgcttctt taaaaatcca
      781 atatcgatct tgatctccgg cctgacgctg aagggctctt cggtctgaag gaggcgttcg
      841 acatactttg caatcggatc gttttccaga ttcacgagcg cgccgacgcc tttgattccc
      901 aatgtcgtat tcttcgcggt gtgagggatg atcgagaccg tgaactgctt gtcgtttaga
      961 tcgttaatcg tgaggctgac gccgtccacc gccacggagc cttttttaat gcagtagcgc
     1021 agtatctctt tcggggcctc gatcgtgaga atgccggcat tctcttcctg ccgccttccg
     1081 gtaaccatcc cgacgccgt
//
LOCUS       DATMKH010000163         1095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3285958, whole genome shotgun sequence.
ACCESSION   DATMKH010000163 DATMKH010000000
VERSION     DATMKH010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3285958"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="VNV63_02050"
     CDS             complement(<1..403)
                     /locus_tag="VNV63_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005504663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HXC61434.1"
                     /translation="MKSEWWRGAIIYQVYPRSFFDANDDGTGDLAGITRKLDYIAQLG
                     VDAIWISPFFKSPMRDFGYDVADYRTVDPIFGTNSDFKSLLSAAHERDLKVIIDMVLG
                     HTSDKHLWFQESCQSPRNPKADWYVWADPNPD"
     gene            complement(505..>1095)
                     /locus_tag="VNV63_02055"
     CDS             complement(505..>1095)
                     /locus_tag="VNV63_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008045258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXC61435.1"
                     /translation="VRAMSDLIAQGKVLYWGTSVWSADEIMEAWTIARQYHLVPPTME
                     QPYYNLLQRNRVEVEYARLYQRIGLGTTIWSPLASGLLTGKYNNGLPKDARLGLESYG
                     WLRDWVLKPERMEQVRKFGKLAAELGLSQSVLAIAWCLKNPHVSTVILGASKVAQLEE
                     NLQASAAAEKLTPDLMTQIDEIMGTRPKEASNSDNH"
BASE COUNT          256 a          287 c          287 g          265 t
ORIGIN      
        1 catcggggtt agggtctgcc caaacatacc aatccgcttt cggatttctc ggggattggc
       61 aactctcctg gaaccaaagg tgcttgtccg aggtatgccc caagaccatg tcgatgataa
      121 ccttcaagtc cctttcatgg gctgctgaaa gcagagattt gaaatccgag ttagtcccaa
      181 aaatcggatc gacagtccgg tagtcagcga catcgtaacc aaaatcgcgc atcggcgatt
      241 taaagaaggg agagatccaa atagcatcaa cacctaattg ggcgatgtaa tcgagctttc
      301 gggtgatacc ggccagatcg ccggtaccat cgtcgttggc atcgaaaaag ctccgcgggt
      361 agacctggta gatgattgca ccacgccacc attctgattt cattgggggt gtattctggg
      421 gggtattttg aaaagcggat tgtccgcagc cggttctaag tctccccgta accgcttgtc
      481 aagcaacctg attagttcat ggactcagtg gttatccgaa ttggaagctt cctttggtct
      541 ggtccccata atctcgtcga tttgggtcat caagtccgga gtcaatttct ctgccgcggc
      601 cgaggcttgg aggttttctt ccaactgggc cactttcgaa gctccgagaa taacggtact
      661 gacgtgcggg ttcttgaggc accaggcaat tgccaacacg gactgggaaa gaccaagttc
      721 agcggccagt ttgccgaatt tgcggacttg ctccatccgc tccggcttta aaacccaatc
      781 ccgcagccag ccgtagcttt cgagtccaag acgggcatcc ttcgggagcc cattgttgta
      841 ttttccggtc agcaacccac tggccaaagg agaccagatc gtggtgccga gtccgattct
      901 ttgataaagg cgtgcatatt cgacttcaac acggttgcgc tggagaagat tgtaataagg
      961 ctgttccatt gtcggaggaa cgaggtgata ttgacgcgca atcgtccagg cttccatgat
     1021 ctcatcagcg ctccagacgc tcgttcccca gtacaagact ttgccctgag cgatcaggtc
     1081 cgacatggca cgaac
//
LOCUS       DATMKH010000164         1259 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1042154, whole genome shotgun sequence.
ACCESSION   DATMKH010000164 DATMKH010000000
VERSION     DATMKH010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1042154"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="VNV63_02060"
     CDS             complement(<1..362)
                     /locus_tag="VNV63_02060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HXC61436.1"
                     /translation="MTPARISNFSYSAERDELPWFRPEDFRAIYADMVRPVVLINGAF
                     DLLHSGHWKLISRARRKAGTLICALDSDARVARKDPRRPIQTFIERATMLGYTPIDYL
                     CEIETDRDMYHLIQVVKP"
     gene            complement(359..>1259)
                     /locus_tag="VNV63_02065"
     CDS             complement(359..>1259)
                     /locus_tag="VNV63_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61437.1"
                     /translation="PTTNTSLAPNPQMPLDLDLTPDVLKYLAQLPAPARTKLLEEIGR
                     QEWDKCASDPMYWIDQSRHALPYVYTRDPKQLYTCKGCSDGNTYTFDSRIRHLQIRHE
                     WEPDRLDVLTSYDFDEHFDGLPTTRPFTLFPYIEPIVKTWLREKILFIEKSRDMMITW
                     LTVTLYTWDTLFHKNRENIFQSDDSSKALDLVERAHFIWDNQPAFLKNVHPVSFSHGQ
                     TRGGILKVPDLKSVIYGFPAGPDQIRQFHPSGCFQDEAAFQAEAEAAFTAIKPAIQAG
                     GRFTAVSSANAGWFHRCVKDVIA"
BASE COUNT          297 a          273 c          389 g          300 t
ORIGIN      
        1 tcgggtttca caacctgaat caggtgatac atatcacgat cggtttcgat ctcacacagg
       61 taatcgatcg gcgtgtaccc caacatcgtt gcacgttcga tgaaggtttg gatgggacgg
      121 cgggggtctt tgcgggcaac gcgggcgtcg gaatcgagag cacaaatcag agtgccggcc
      181 ttgcgacggg ctcgggagat cagtttccaa tggccggagt gcaggagatc gaaagcaccg
      241 ttaatcagaa ccactggtct taccatatct gcgtagatcg ctcggaagtc ttccggacgg
      301 aaccacggga gttcatctcg ttctgcggag taggagaagt tggagatgcg ggcgggggtc
      361 atgcaatcac atccttgaca cagcgatgga accagccggc gttggcagag ctgaccgcgg
      421 tgaaacgtcc acccgcctga atagcaggtt tgatagcggt gaaagcggct tcggcttcgg
      481 cttgaaatgc agcttcgtcc tgaaagcaac cggaggggtg gaactggcgg atttggtcgg
      541 ggcctgcggg gaaaccataa attacggatt tgagatctgg cactttcaga attccgcccc
      601 gagtctgccc gtgagagaaa ctaactggat gaacgttctt cagaaacgcc ggttgattat
      661 cccagatgaa atgagcacgt tctaccaaat ccagagcttt ggaggagtcg tcggattgaa
      721 agatattttc acggttcttg tgaaacaggg tatcccaggt gtatagagta actgtcagcc
      781 aggtaatcat catatcccgg cttttttcaa taaacagaat cttctcccgc agccacgttt
      841 ttacgatggg ttcgatgtag gggaagagag tgaaaggcct tgtagttgga agtccgtcga
      901 agtgctcatc gaagtcgtaa gaagtaagaa cgtcaagacg gtcgggttcc cattcgtgtc
      961 gtatctgcaa atgtcgtatg cgcgaatcga acgtgtaggt gtttccatca gaacagcctt
     1021 tgcaagtata gagttgtttg ggatcacggg tgtagacata ggggagagcg tgacgggatt
     1081 ggtctatcca gtacatcggg tccgatgcac acttgtccca ttcttgacga ccaatttctt
     1141 cgaggagttt ggttctggcc ggtgcgggaa gttgggcgag gtacttcagt acgtcagggg
     1201 tgagatcaag gtcgaggggc atctgtggat tgggtgccaa ggaggtattc gtggttggg
//
LOCUS       DATMKH010000165         1029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_80147,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000165 DATMKH010000000
VERSION     DATMKH010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80147"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..940
                     /locus_tag="VNV63_02070"
     CDS             <1..940
                     /locus_tag="VNV63_02070"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXC61438.1"
                     /translation="AQLKAQGISIIYVSHKLEEIKQIGDRVTILRDGTHIATLNLRDA
                     ELREIVRLMIGREMPIEQRERTGRQSDIVFALEDVTNQHFVRPFNLGLRANEILGITG
                     LVGAGKTELARAIFGVDKMVTGKMYLQGKEIKVSSPRHAVRSGIGYLPEDRDSEGLCL
                     NMGVRENLTLALLAKLHRVFLDRASETRLAKETIKSVKIRTSGVSQQVKYLSGGNKQK
                     VIFAKWLEANCQVLILDEPTMGIDVGARKEIYDLVRRFIKKPQKAVIFISSDIQEILE
                     IADWILVMSDHRVVAELNAGETNKQEIVEYSMRLTH"
BASE COUNT          288 a          217 c          304 g          220 t
ORIGIN      
        1 cgcgcaatta aaagcacaag gaatcagcat tatctatgtc tctcataaac tcgaggaaat
       61 aaagcaaata ggagatcggg taaccattct ccgtgatggc acccatattg ccactctcaa
      121 tctgcgggat gcggagttaa gggagattgt ccggctgatg atcggtcgag agatgccgat
      181 tgagcaaagg gagaggaccg gtcgtcaaag cgatattgtc ttcgcgctgg aagatgtcac
      241 gaaccaacac tttgtgcgtc cattcaatct agggctgcgt gccaacgaaa ttttgggtat
      301 caccggcctg gtgggcgccg gaaaaacgga attggcccgg gcgatctttg gggtggacaa
      361 gatggtgacg ggcaaaatgt atctgcaggg aaaagagatc aaggtcagta gccctcgtca
      421 cgcggtgaga tccgggattg gctacctgcc agaagatcga gatagtgaag ggctgtgtct
      481 taacatggga gtacgggaga acctgaccct ggcgcttctg gcgaaactgc atcgggtatt
      541 cctggatcgt gcttccgaga cgagactagc gaaggaaacg atcaaatcgg tcaagatcag
      601 aacgagcggt gtttcccagc aggtgaaata cttaagcggt ggcaataaac aaaaggtgat
      661 ctttgccaaa tggctggagg ccaattgcca ggtattaatt ctggatgaac cgaccatggg
      721 cattgatgtt ggagctcgga aggaaatata cgatctggtg cggcggttca tcaagaaacc
      781 gcagaaagcc gtgattttta tttccagcga cattcaagaa atattggaaa ttgctgattg
      841 gatcctggtg atgtcagacc accgtgtcgt tgccgagctg aacgcgggcg agacgaataa
      901 gcaggaaatt gtcgaataca gcatgcggct gacccactga gcggcagagg ttgagaaaaa
      961 caagctcagg gcgaaagact aatcggatga tctcacgaca gattcgcgct gcgtttagta
     1021 atcaagcgg
//
LOCUS       DATMKH010000166         1099 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4558857, whole genome shotgun sequence.
ACCESSION   DATMKH010000166 DATMKH010000000
VERSION     DATMKH010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4558857"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(358..>1099)
                     /locus_tag="VNV63_02075"
     CDS             complement(358..>1099)
                     /locus_tag="VNV63_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HXC61439.1"
                     /translation="NEAFLRETKASLEKHGLQMPMLCCSPDFTEPDPDLLEEQIARER
                     RMIEITAFFGGRFCRVLSGQRRPGLSIETGIAQVVRVIKSLLPCAEKHGVVLSMENHY
                     KDSYWRYPEFAQKMDIFTAIIEQIDSPWFGINYDPSNTILAGEDPLEMLERVKHRVVS
                     MHASDRYLNNGTLEDLRREEDAVGYASRLSHGIIGRGLNDYDKILSTLNSVGFSTWVS
                     IEDGMNGIEDMRESVRFLREKIDLHFAG"
BASE COUNT          255 a          320 c          271 g          253 t
ORIGIN      
        1 cgccgtccgg cctccatctc ctctccggcc gcggcaactt tggaagattt accgggtcga
       61 tcggccactg aacccgtttt cgtctggggg gacttactca gagacattcg ctaagcaata
      121 gcaccgcttg ggacccgtgt cagttggttg tcggttgctc tctcaatggt cggttgtcag
      181 ttgtcagccc gttcgacgcg cttcctcatg tggcgatggc ctgccttgcc agagggagag
      241 gaagagcaca tcgaaaaacg aatgcctcct ggcggatacg tcaatctggc cgtccctcgt
      301 gaaagtccgc cctctctacc gaccaacaac tgacaaccga caactctttt ttcgatctca
      361 gcccgcaaaa tgcagatcga tcttctcccg cagaaatcgt acagattccc gcatatcctc
      421 gatgccattc attccatcct cgatcgagac ccaggtggaa aacccaaccg agtttaaggt
      481 ggaaagaatt ttgtcatagt cgttgagccc acgacctata atcccgtgcg agagccgact
      541 ggcataaccg acagcatcct cctctctccg gagatcctcc aacgtaccgt tgttgaggta
      601 gcggtcactg gcgtgcatgg agacgacccg atgcttcaca cgctcaagca tttccaaagg
      661 atcctcaccg gcgagaatcg tattggaagg atcatagtta atgccaaacc atggagaatc
      721 gatctgttcg atgatcgcgg tgaaaatatc cattttttgc gcgaattcgg gataacgcca
      781 atagctatcc ttgtaatgat tttccatcga caaaacgacc ccatgcttct ccgcacatgg
      841 caaaaggctc ttaatcactc ggacgacttg ggcgatgccg gtttcaatcg atagcccggg
      901 gcgccgctgg ccagacagga cacgacagaa acgcccgcca aagaaggcag tgatttcgat
      961 cattcgcctc tcgcgggcaa tctgctcctc cagcaagtcg ggatcgggtt cggtgaagtc
     1021 cggcgagcag caaagcatcg gcatctgaag tccgtgtttt tccaacgatg ctttggtctc
     1081 ccgcaaaaat gcttcgtta
//
LOCUS       DATMKH010000167         1275 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4247586, whole genome shotgun sequence.
ACCESSION   DATMKH010000167 DATMKH010000000
VERSION     DATMKH010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4247586"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="VNV63_02080"
     CDS             complement(<1..556)
                     /locus_tag="VNV63_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61440.1"
                     /translation="MSEKKDLRVLDPFCGVGTTLIGSQELSNGINISAVGIERNPFIQ
                     FVAQTKVRWPEMDSAIILKIGTEILTKIGTSEGQIPELSSLRTGRCMSRHVAGRLVAI
                     IDSIRSHGRTPNHDALILGVAASAEQLSKIRKDGRALRLVKKNRQLTNKIVQEKWQEI
                     ASDVRFFQQIIPRASIPTVILGDGR"
BASE COUNT          282 a          362 c          299 g          332 t
ORIGIN      
        1 atcttccatc tcccaaaatg acggtcggaa tggacgcgcg agggatgatc tgctggaaga
       61 acctgacatc cgaggcaatt tcttgccact tttcctgcac aatcttgtta gttaattgcc
      121 gatttttctt gacgagacgc agcgcccgtc catctttgcg gattttgctg agttgttccg
      181 ctgaggctgc gacgccaaga atgagcgcat cgtgattggg tgtcctcccg tgagaccgga
      241 tggagtctat gatggctacc aaacgcccag caacatgtcg gctcatgcat cgcccagttc
      301 tcagacttga cagctccgga atttgccctt ccgatgtgcc gatctttgtc agaatttccg
      361 taccaatttt caaaatgatg gcagaatcca tctccggcca cctgaccttg gtttgagcga
      421 caaactggat gaaaggattt cgttcgatac cgaccgcaga aatgttaatt ccattggata
      481 actcttgact cccgattagg gtcgtcccga ctccacagaa agggtccaac acccgcagat
      541 ctttcttttc cgacaaggcg agtgattcta ggacggttcg caagaggtcc gcggaaaagc
      601 tctctttgaa cttgaaccag cgatgtacgg gcaccctttc atttgctccg gacacaacta
      661 gaggctcgaa atccgacgcc gtttcgatcg gcaccgctct ttcaattttt cgatacatcc
      721 taagttctgg caattgcttt tctgagatca tttgtgtggg tgttgagtct tgacagcaaa
      781 cgctcgaatt cgtcttacgt ctctctcagt gaaaaggcgc cagccatttc tgtctcttga
      841 cacttccccg acccttccgg agagcaacca tcgcctgagc gtgatcgggg caactccaac
      901 ctgctgagcg acctcccgca gtgtgtgaaa cttcttgtct cttctcttca tggttcgatc
      961 cgattcagac cggaactgat atatatcaac atacatcagg ttatgtctta cggacaataa
     1021 tttccagttc gggacgggcg gccaaccctt ccgcatgtat tcagttgatg aaatctcgcg
     1081 gtcggattat ggcagggcaa tacgtcaacg ccgagttact aatttcggtc tttcgggtgt
     1141 ccgacctttc cggcgctagt gtcggggttt tgacgtcctt cccggaaaac tcaaatcctc
     1201 cgacccctca aaaaccacga aggatcgggc gcccgcaact tcgccctcga ctcaagagcc
     1261 cggcccccgc caccc
//
LOCUS       DATMKH010000168         1060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_401824,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000168 DATMKH010000000
VERSION     DATMKH010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401824"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /gene="ispE"
                     /locus_tag="VNV63_02085"
     CDS             complement(<1..569)
                     /gene="ispE"
                     /locus_tag="VNV63_02085"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HXC61441.1"
                     /translation="MRIATPAKINLTLEVLGQRSDGFHELATWMIPIGLYDELSLEVS
                     SEMTYETNLPNLAFDHTNLILRAIERFQEATGRSAVYRVGLRKEIPIGAGLGGGSSDA
                     AATLVLLNRLHENPLAGDVLLNLAAQLGSDVPFFLKGCSAWCTGRGEKMEIREFPTDR
                     WVVLIKPEFGVSTAAAYAAYKGLPPDQKRG"
     gene            583..>1060
                     /gene="pyrF"
                     /locus_tag="VNV63_02090"
     CDS             583..>1060
                     /gene="pyrF"
                     /locus_tag="VNV63_02090"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF012441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HXC61442.1"
                     /translation="MEESLTAKDKIIVALDLPSVDTARKLISLLGEDVSWYKIGLQLF
                     ALGGPPFVQEIKSSGAKIFLDLKFHDIPNTVRSAVESACSLRVDMLTIHLSGGSEMCQ
                     AAVKGRGATSTQILGVTVLTSQTEVTLSEIGIQRSLTQQVLSLATIAKTTGVTGLIA"
BASE COUNT          262 a          315 c          245 g          238 t
ORIGIN      
        1 cctcgctttt ggtccggtgg caaccctttg taggccgcat aagccgctgc cgtggaaacc
       61 ccaaactcag gtttgatcaa gacgacccac cgatcggtcg ggaactcgcg aatctccatc
      121 ttttcacccc ggcccgtgca ccaggctgaa catcccttta gaaagaacgg aacatctgac
      181 cccagctgag ccgcgaggtt aagaaggacg tcaccagcaa gcggattttc gtgcaatcga
      241 tttaacaaaa ccaaggtggc agcggcgtca ctgcttccgc cgcctaatcc cgcgccgatc
      301 ggaatttcct tgcgcagacc cacccggtaa accgcgcttc gcccagtcgc ttcctggaac
      361 cgttcaatcg cccgcaggat cagattcgta tgatcaaaag ccagattagg aaggttcgtt
      421 tcgtaggtca tctcggacga cacttccagt gatagctcgt cataaagacc gatcggaatc
      481 atccacgtcg ccagctcatg aaatccgtcc gatctttgcc ccagtacttc aagcgtaaga
      541 ttaatcttgg ccggcgttgc aatcctcata cagagtcacc ctgtggaaga atccttaaca
      601 gcgaaagata aaatcattgt cgcactcgat cttccgtccg tcgacacggc tcgcaagtta
      661 atctcacttc tgggcgagga cgtttcttgg tacaagatcg gactacaact ctttgcacta
      721 ggcggcccac ccttcgttca ggagatcaaa tcatccggcg caaaaatctt tctcgatctg
      781 aagttccacg atattccaaa tacggtccga tccgcggtgg aatcagcgtg ttctttaaga
      841 gtcgatatgc tgacgatcca tctttctggc ggatcagaaa tgtgccaagc agcagtaaag
      901 ggtcggggtg caacctccac tcaaatcttg ggtgtgaccg tcctcaccag ccaaaccgaa
      961 gtcacgcttt ctgaaatcgg tatccaacga tcattgaccc aacaggttct ctccctggca
     1021 acaatcgcaa aaacgacggg tgtcaccggc ctgatcgcta
//
LOCUS       DATMKH010000169         1130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1362210, whole genome shotgun sequence.
ACCESSION   DATMKH010000169 DATMKH010000000
VERSION     DATMKH010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1362210"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(181..>1130)
                     /locus_tag="VNV63_02095"
     CDS             complement(181..>1130)
                     /locus_tag="VNV63_02095"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXC61443.1"
                     /translation="ESNPGIIIGTSPAMQPVFDLIAKVAASDVTVLITGESGTGKELV
                     ARAIHKRSERRNEPFVTVNCCAIPENLLEAELFGHEKGAFTGAHQQRHGKFELAHQGT
                     IFLDEIGDMSLSMQTKILRVLQEKEIEPVGGSGPIKVDLRLLAATHRNLQAAIENGSF
                     REDLYFRLNVVNIPLPPLRERTEDLKDLTDYFIKKYNQRFQKTVKGLTERAKKLLLSY
                     HWPGNVRELENLIQRTIVLTEEQELSDELIGGFLKRETDDAIGSLKEKVGKSSDEIEK
                     RTILEALGETGWRREETAKRLKISRKNLYNKIKKYGLLE"
BASE COUNT          246 a          299 c          256 g          329 t
ORIGIN      
        1 aacatgttta ggccatttcc gcaaccccgg tttttttgct ctcgctagag gagatggcgt
       61 gggtatctta tttagttgaa ggtgtaatct cgtaagtgtt tgctgggttt gatcgggaaa
      121 tttatcttaa cagattggtt cctcttaaaa aggtcgaagc gaggaaaagt agcctgttcc
      181 ttattctaac aatccgtact tctttatctt attgtagaga ttcttccggc tgattttaag
      241 acgtttggct gtctcttctc gcctccagcc cgtttcgccg agagcttcca gaatggttct
      301 cttctcgatt tcatccgatg actttccgac tttttctttc agcgagccga tggcgtcatc
      361 tgtttctcgt ttgagaaatc cgccgatcag ttcatccgaa agctcctgct cttcggtcaa
      421 gacgatcgtt cgctgtatga ggttttccag ctcacggaca ttgccgggcc aatgatagga
      481 caaaagaagt ttcttggctc tctccgtcaa acctttaacc gtcttctgaa acctctggtt
      541 gtatttcttt ataaaataat cggtcaaatc tttaagatcc tccgttcgtt ccctcaaagg
      601 cggcaaagga atgtttacca cgttgagccg gaaataaagg tcctcacgaa agcttccgtt
      661 ttcgatcgcc gcctgcaggt tccgatgcgt cgcggccaac agccggagat ctaccttgat
      721 cgggccgctt ccacccaccg gctcgatctc tttttcctga agcacccgca ggatcttggt
      781 ctgcatcgaa agactcatgt ctccgatttc atctaaaaag atcgtaccct gatgagccag
      841 ctcaaatttt ccatgacgct gctggtgtgc tccggtgaaa gctcctttct cgtgaccaaa
      901 cagttccgct tccaaaaggt tttcaggaat cgcacaacaa ttcacggtaa caaagggctc
      961 attccgccgt tcactccgtt tatgaatggc tcgagcgacc agttccttgc cggtcccgga
     1021 ctcaccagtg atcaggaccg taacatcact cgcggccact ttagcgatca ggtcaaagac
     1081 cggctgcatg gccgggctgg taccgatgat tatcccggga ttggactcgg
//
LOCUS       DATMKH010000170         1511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2647153, whole genome shotgun sequence.
ACCESSION   DATMKH010000170 DATMKH010000000
VERSION     DATMKH010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2647153"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1015
                     /locus_tag="VNV63_02100"
     CDS             <1..1015
                     /locus_tag="VNV63_02100"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HXC61444.1"
                     /translation="TIDRKKEANIMKNTDNAPVRCAIILAGGEGRRLRPFIQRLRGDA
                     LPKQYVNFIGTRSMLEHTYHRAEKLITPENLFTVVTKNHLKYDDVYPQLSNRPKGTVI
                     IQPENKDTGPGLLLPLMHLIKRYPESVVVIFPSDHFIVEEDLFMGHVDLACRVVERDA
                     SLLVLLGVEPYGPEPEYGYILPDGKMNRPTPSGICRVSRFIEKPGPHVARELVQRGGL
                     WNTLVMAFKAKTILDLVRRAVPVLYQSFQLIGRTLGTAREMDVVDEAYRKMEPLNFSK
                     ELLEILPIQGLLRLSVLPVRGVFWSDWGSERRILSDVQKFRYEEGLYGLGRSDINRFS
                     LQA"
     gene            1044..1244
                     /locus_tag="VNV63_02105"
     CDS             1044..1244
                     /locus_tag="VNV63_02105"
                     /inference="COORDINATES: protein motif:HMM:NF026065.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HXC61445.1"
                     /translation="MTAKTEKSGNVQSKGAQEIHVGDEVYLYKDSGIRERHGVIPLWL
                     QLVSYGLIIWGIYYTIRYWSTG"
     gene            1296..>1511
                     /locus_tag="VNV63_02110"
     CDS             1296..>1511
                     /locus_tag="VNV63_02110"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HXC61446.1"
                     /translation="MKPKFETVGQIKKGNTLFTYEDTPISKIAEDLAHSRWTGMPVVD
                     QKNRVIGIVSEQDLLRAFRGNRPLEEIK"
BASE COUNT          392 a          359 c          417 g          343 t
ORIGIN      
        1 cacgattgat cgcaagaagg aggctaacat catgaagaac acagacaatg caccggttcg
       61 ttgcgcaatt atcttagccg gcggggaagg caggcgactt cggccgttta ttcaacgcct
      121 acgtggcgat gcacttccca aacagtacgt caatttcatc ggaacgcgct ccatgctgga
      181 acacacttat catcgtgccg agaaactgat cacgcctgaa aacctcttta cggttgtaac
      241 gaagaatcat ctcaagtatg atgatgtcta cccgcaactt tcgaatcggc cgaaaggaac
      301 cgtcatcatc caaccggaaa ataaagacac gggtccgggg cttctcctcc cgctgatgca
      361 tctgatcaaa cgctatccag aatctgttgt cgtaattttt ccttcagatc atttcatcgt
      421 agaggaggac ctcttcatgg gccatgtgga tctggcctgc cgtgtggtgg agcgggatgc
      481 ttcccttttg gtcctgttgg gggtggagcc gtatgggccg gagccggaat atggatatat
      541 cctaccagat gggaagatga atcgtccgac cccctcgggc atctgcaggg tgtcacgatt
      601 catcgaaaaa ccgggaccgc atgttgcccg cgagctggtt cagaggggcg ggttgtggaa
      661 cacattggtc atggccttta aagccaagac aatcctggat ctggtgcgca gggcagttcc
      721 ggtcctctac caatcctttc agctgattgg aagaacgctc ggcacagcca gggaaatgga
      781 tgtggtggat gaagcctacc gaaagatgga gccgttgaat ttttcgaagg aattgctgga
      841 aattcttccg atacagggtc tattgcgtct atccgtcctc ccggtccgag gggtcttctg
      901 gagcgactgg gggtcggaac gccgtatttt gagtgatgtg caaaaattca ggtatgaaga
      961 agggctgtat ggactcggac gatcggacat taaccgattc agtctacaag cataacaagg
     1021 atgaagataa gggggtgggt acaatgactg cgaagactga aaaatctgga aatgttcaat
     1081 cgaaaggagc ccaggagatc catgtcggcg atgaggttta cctctacaag gactccggca
     1141 tccgggaacg gcacggtgtg atcccgctct ggcttcagct cgtctcctac gggctaatca
     1201 tctgggggat ttattatacg atccggtact ggagtaccgg atagcctgaa ggagccctgt
     1261 tgacttttta aacggttaac cataaggagg gaaacatgaa acccaaattt gaaaccgtcg
     1321 gacagatcaa aaagggaaat acactgttta cctatgagga cacgccgatc tctaaaattg
     1381 ccgaagatct cgctcacagc cgctggaccg gaatgccggt ggtggatcaa aagaatcggg
     1441 tcatcggcat cgtcagcgag caagacctat tgcgagcctt cagagggaat cgtcccttag
     1501 aggaaatcaa a
//
LOCUS       DATMKH010000171         1200 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4561229, whole genome shotgun sequence.
ACCESSION   DATMKH010000171 DATMKH010000000
VERSION     DATMKH010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4561229"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..993
                     /locus_tag="VNV63_02115"
     CDS             <1..993
                     /locus_tag="VNV63_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61447.1"
                     /translation="GETWEEYLAGHLGEPIRNFGVGGYGVYQAYRRMVREENTDHAAK
                     YLILMICCDDAIRSLYRVRWAAIYPWFREEAETIHMFHGNYWDHMEMDLDTGRFVEKK
                     NLLSTEQSLYHMTEPQWMAEHLNDDLALQLEVYRRGFIRDLDRDRVSKLAARLDFPFD
                     WSLASKPGTVTSRWSDTPETLMQFQAGELLNRYSQRATIFVLDEARAFARKNGKKLLI
                     ILNGTVDLDRLKQQGTREDQEVLDYMAKERFDYVDLNMVFFRDFQKSNLTAEQFMNTY
                     LVNEAHLNPMGNHLIAYAMKDKVVEWLDPRPVPYQQPETQIINFKGYLHGGVYH"
     gene            1001..>1200
                     /locus_tag="VNV63_02120"
     CDS             1001..>1200
                     /locus_tag="VNV63_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61448.1"
                     /translation="MNQLRLQILFAMVSSTLLWTSASALAQSVSPFAPDKMDSILYGV
                     AYYTEYMPYERLDKDVEMMQQAG"
BASE COUNT          297 a          314 c          325 g          264 t
ORIGIN      
        1 ggcgagacct gggaggaata tctggcaggg cacttgggtg agccgattcg gaacttcggc
       61 gtcggaggat acggagtcta tcaggcatac cggcggatgg tccgcgagga aaataccgat
      121 catgccgcca agtacttaat cctgatgatt tgctgcgacg atgcgatccg gagcttatac
      181 cgagtgcgct gggccgcaat ctatccctgg tttcgtgaag aagcagaaac cattcatatg
      241 ttccacggca attattggga tcacatggag atggacctcg atacgggccg cttcgtcgaa
      301 aagaaaaacc tgctctctac cgagcagtcc ctttatcaca tgacggaacc acaatggatg
      361 gcggaacacc ttaacgatga tctggctctg caattggagg tctaccgcag aggcttcatc
      421 cgggatctgg atcgggatcg ggtcagcaaa ctggcggcac gtctcgattt tccgttcgat
      481 tggagccttg cgtccaagcc cggcacagtg accagccgat ggtcggatac gccggaaaca
      541 ctcatgcagt tccaggcagg cgaattgttg aatcgctact cgcagcgcgc gaccatcttc
      601 gttctggacg aggccagagc ctttgcccgg aaaaacggaa agaagctact gatcatattg
      661 aacgggaccg tggacctgga cagactgaag cagcaaggta ctcgcgaaga ccaggaggtc
      721 ctggattaca tggcgaagga acgattcgac tacgtagacc tgaacatggt cttctttcgc
      781 gatttccaaa aatccaattt gaccgccgag cagttcatga atacctatct tgtcaatgaa
      841 gcccatctca accccatggg gaaccacttg attgcctatg ccatgaagga caaggtggtg
      901 gaatggctag accccaggcc tgtcccctat cagcagcccg agacacaaat catcaacttt
      961 aaaggttact tacacggtgg ggtttaccac tgatcacgaa atgaaccaac ttcggctgca
     1021 gattctcttc gccatggtta gttccacgtt gctgtggacc tccgcgtccg cgcttgccca
     1081 atcggtttct ccctttgccc cggacaaaat ggattccatt ctttacgggg tagcttacta
     1141 cacggaatat atgccgtatg agcggctgga caaggatgtc gagatgatgc agcaggcagg
//
LOCUS       DATMKH010000172         1346 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2006236, whole genome shotgun sequence.
ACCESSION   DATMKH010000172 DATMKH010000000
VERSION     DATMKH010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2006236"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="VNV63_02125"
     CDS             complement(<1..293)
                     /locus_tag="VNV63_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61449.1"
                     /translation="MKTSINLIAEILERERFPTEKLLLPAASFLLLLAILMASAWEIL
                     NARTMDRQFKQLVVQRDALTTEVTRLTRDIMNFQGQETTEQQEAGKKLTTVKSL"
     gene            complement(310..>1346)
                     /gene="pilM"
                     /locus_tag="VNV63_02130"
     CDS             complement(310..>1346)
                     /gene="pilM"
                     /locus_tag="VNV63_02130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HXC61450.1"
                     /translation="GIDLGRSSIKLVQLRSTRTGFRLIYAGLTELPVIGSPLTDPAIS
                     LALMDLLQKGRLKSEKAAVNFIGNTPVIRYITLPKIPKEELKEAVKWEAKKIVPLPIE
                     EMVLDFLTVGEQEDREVKRLELVVVAAERSEVLAQYNELKRIGLNVAAVDVNPLALLN
                     AVCLNHAADLNDNIVFVDIGGGKTDINITKQGVLRFTRNVQIGGEEITKAIERELHVG
                     REEAETMKRQRGMLSKGAGTDIPDADGRLKDVIQREADRIILETQRSIDYYRAQFREG
                     EIKKIILMGGTPLLPGFQDYFSAYFGTKVAMDDPFAEIDCDESSFAEFRIMAPRFSTS
                     VGLALRKGDV"
BASE COUNT          286 a          412 c          327 g          321 t
ORIGIN      
        1 aggctcttga ccgttgtcaa cttcttcccc gcctcctgct gctcggttgt ttcctgaccc
       61 tgaaagttca tgatgtcccg cgtcagccgg gtaacctctg tggtcagggc atcccgctgc
      121 accacgagct gcttaaattg ccggtccatc gtcctcgcgt ttaggatctc ccaggcagag
      181 gccatcaaga tggcaagcag aagcagaaac gaggccgccg gcaacaagag tttctccgtc
      241 ggaaaccgtt ctctctccag gatctcggca ataagattga tcgaggtctt cattgaattt
      301 ccttgggtct cacacgtccc ccttgcggag agccaaaccg acactggtgg agaaccgggg
      361 agccattatt ctaaactcgg caaaggaaga ctcatcgcag tcgatctccg cgaaaggatc
      421 atccatcgcc accttggttc caaagtatgc ggagaagtag tcctgaaaac ccggcaagag
      481 cggcgtaccg cccatcagaa tgatcttctt gatctctccc tccctgaact gtgcacggta
      541 ataatcaatt gaacgctggg tctccagtat aatgcggtcc gcctctcgct gaatcacatc
      601 tttcaagcgt ccatcggcgt ctggaatatc agtcccggcg cctttcgaca gcattccacg
      661 ttgccttttc atcgtctccg cttcttcgcg gcccacatga agttcccgct caatcgcttt
      721 tgttatctcc tctccgccga tctgaacgtt ccgcgtgaac cggagcacgc cttgtttagt
      781 gatattgata tcggtcttgc ctcctccgat atccacaaag actatgttgt catttagatc
      841 ggccgcgtgg ttgaggcaga cggcattgag cagcgcaagc gggttgacat ccacggcagc
      901 gacattgagc ccgatccgct tcaactcatt gtactgggca agcacttcgg atcgctccgc
      961 cgccaccacg accagctcca ggcgtttaac ctcccggtcc tcctgctccc cgacggtcag
     1021 aaaatccagc accatttcct cgatcggcag cggcacgatt ttcttagctt cccatttgac
     1081 cgcctctttc aattcttcct tcggaatctt gggaagcgtg atgtatcgaa tgaccggcgt
     1141 atttccgatg aaattgacgg ccgccttctc gctcttcagc cttcccttct gcaagagatc
     1201 catcaaggca aggctgatgg ccggatcggt gaggggagac ccgatcaccg gaagctcggt
     1261 cagacccgca tagatgagcc gaaaacctgt tcgcgtggaa cgaagctgaa ccaacttgat
     1321 cgagctgcga ccaaggtcga ttccga
//
LOCUS       DATMKH010000173         1606 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_84276,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000173 DATMKH010000000
VERSION     DATMKH010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84276"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..825)
                     /locus_tag="VNV63_02135"
     CDS             complement(<1..825)
                     /locus_tag="VNV63_02135"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXC61451.1"
                     /translation="MITKSYEFLIGLRYLKAKRRRRSVSLNTFISIGGVTLGVAALIA
                     TLAVMTGFKEDLRDKILGTNAHVIITDRTSNAIKDYSKIITQVQQLPHVVAATPFIYN
                     QVLLSSEAAVHGVVLRGIDPRSEARVTDIRKNMIDGSLEALEKKTPAEDGKDKPNLPG
                     IIIGKELAARLGLLVHDKLNVMSPVGTMGPMGIIPKIRKFEVVGVFDSGMYEYDSTLA
                     FVSLGTAQDFFNMGDVVTGVEVKVDDIFIADRVASEIESKLGFPLGARDWMKLNRNL"
     gene            complement(830..>1606)
                     /locus_tag="VNV63_02140"
     CDS             complement(830..>1606)
                     /locus_tag="VNV63_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HXC61452.1"
                     /translation="GGFERVYEINRSFRNEGISTVHNPEFTILEFYMAYADYQTLIQF
                     TEELITTVALKVMGKLRLEFKNQTIDFTPPWKQKTFIQAIAEANSLDAKFLNDRSHLL
                     ALAKKLELPIKGSEPSGKILDLLFETTVQPKLIQPTFILDYPIEISPLARRKTNEPEL
                     TERFELFIAGREVANAFSELNDPQDQRNRFEQQMAQRAAGDLEAPPLDEDFLRALEYG
                     MPPAAGEGIGIDRFVMVLTNQPSIRDVILFPQMKPESQRG"
BASE COUNT          329 a          451 c          425 g          401 t
ORIGIN      
        1 gagattccgg ttgagtttca tccagtcccg tgcccctaac gggaatccga gcttgctctc
       61 gatctcgctc gcgacccggt cggcaatgaa aatgtcatcc accttgacct cgaccccggt
      121 taccacgtcc cccatgttaa aaaagtcctg ggccgtaccc agtgagacaa aggcgagagt
      181 cgagtcatat tcatacatcc cggaatcgaa gacacctaca acctcgaatt tccggatctt
      241 cgggatgatt cccatcgggc ccatggttcc caccggggac atcacattga gcttgtcgtg
      301 aacgaggagc cccaatcgtg cggccagttc ctttccgatg atgatgcccg gcaggttagg
      361 cttgtccttg ccgtcctcgg ctggagtttt tttctcgagc gcctccaggc tcccatcgat
      421 catgtttttt ctgatatcgg tgacacgtgc ctcggatcgc gggtcaatcc cgcgcagaac
      481 cacgccgtgc actgcggcct ccgaggaaag cagaacctgg ttgtagataa acggcgttgc
      541 ggccacgaca tgaggcaact gctgaacctg agtgatgatc ttcgagtaat ccttgatcgc
      601 gttgcttgtc cgatccgtga tgatgacatg agcgttggtg cccagaatct tgtcgcgcag
      661 gtcttctttg aacccggtca ttacagcgag ggtcgcgatc agcgccgcaa cccccagtgt
      721 cactccgcca atcgagataa atgtgttcag cgagacgctc ctccgccggc gtttggcctt
      781 gagatatcgg agaccgatca agaattcata cgatttggtg atcaaagttt tatcctcttt
      841 gagattccgg cttcatttgt ggaaacagta tcacgtcacg gatgctgggc tggttggtta
      901 acaccatgac gaaccgatcg atcccgatgc cttcaccagc cgccggcggc atcccgtatt
      961 ccaatgcccg caggaaatct tcatccaacg gcggagcttc gagatccccc gccgctctct
     1021 gggccatctg ttgttcgaag cggtttcgtt gatcttgggg atcgttgagt tccgagaagg
     1081 cgttggccac ctcgcggcct gcgatgaaga gttcgaaccg ctccgtgagt tccggctcat
     1141 ttgttttgcg cctggccagg ggagagatct caatcgggta gtccaaaatg aaggtgggct
     1201 gtatgagctt cggttggacc gtggtctcaa acagcaagtc caggatcttt cccgaaggtt
     1261 cgcttccctt gatcgggagc tctaattttt tagccagcgc caacagatga gaccggtcgt
     1321 tcaggaattt cgcatcgagc gaattggcct ccgcaatcgc ctggatgaat gtcttctgtt
     1381 tccacggagg cgtgaagtca atcgtctggt tcttgaattc cagccgcagc tttcccatca
     1441 ccttcagggc gactgtcgtg atcagctctt ccgtgaattg gataagcgtt tgataatcgg
     1501 cataggccat gtagaattcc agtatcgtga attcgggatt gtgcacggtt gagatcccct
     1561 cattccgaaa gctccggttg atttcgtaga cacgttcaaa cccccc
//
LOCUS       DATMKH010000174         1270 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3290892, whole genome shotgun sequence.
ACCESSION   DATMKH010000174 DATMKH010000000
VERSION     DATMKH010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3290892"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1165
                     /locus_tag="VNV63_02145"
     CDS             <1..1165
                     /locus_tag="VNV63_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007293369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXC61453.1"
                     /translation="HDFNNLLGVILGYIQVIKRDLVSGNSMYEYAEEIEKASRRAVSL
                     TRQLLAFSRQQVLEPVILNLNTLVSEVEKMLPRLIGEDIELKLILDPAIGQVKADPGQ
                     LEQVLMNLAVNARDAMPDGGKLLIQTGGAEIDAAFAREHPGSVPGKYVTLAVTDTGTG
                     MDPETQAQIFEPFFTTKGRDRGTGLGLATVYGVVKQSNGYIAVDSEIGKGSLFTVYLP
                     LIELAVTQPIESCPEPLTIRGSETILLVEDAEPLRNLAKLFLKENGYRVLTAADGSEA
                     QKVAAQISGPIHLLLTDMVMPGINGRVLAERLAPRHPAMKVLYMSGYTDSFIAGHGVL
                     EPGTHLLHKPFTEDVLVRKVRELLDEGRDTKKTDSVEGTESPVGAGSDIKLKR"
BASE COUNT          313 a          325 c          364 g          268 t
ORIGIN      
        1 ccacgatttc aacaatctcc ttggagttat cctcggctac attcaggtca tcaagcgaga
       61 tctggtttcc ggaaattcca tgtatgagta cgctgaagaa attgagaaag ccagccgccg
      121 cgctgtgtct ttgacccggc agcttttggc cttcagccgt caacaggttc tggaaccggt
      181 gattttgaac ctgaacacgc tggtatccga agtggaaaag atgttgcccc gcctgatcgg
      241 agaagacata gagctcaaat tgatcctgga cccggcgatc ggccaggtca aagccgaccc
      301 cggacaactg gaacaggtct tgatgaatct ggccgtcaac gcgcgtgatg ccatgcccga
      361 tgggggcaaa ttgttgatcc agaccggcgg cgctgaaatc gatgcggctt ttgcaaggga
      421 gcatcccggt tcggtccccg gcaaatacgt aacgctggcc gtcacggata ctggcacagg
      481 gatggacccg gaaacccagg cgcaaatttt cgagccgttt ttcaccacca agggacgaga
      541 cagaggaacg ggactgggtc tcgccaccgt gtatggcgtg gtgaaacaaa gcaacgggta
      601 tatcgccgtt gattcagaga taggtaaagg atctttattc acagtctatt tgccgctgat
      661 cgaactagcc gtcactcaac caatcgagag ctgtccggag ccactgacga tccgtggatc
      721 ggagaccatc ttgttggtcg aagacgcgga gcctttgcgg aacttggcga aattgtttct
      781 gaaggaaaac ggatatcggg tcctgaccgc cgcggatgga tcggaagctc agaaggtagc
      841 tgcacaaatc tccggcccga ttcatctcct gctcaccgac atggtcatgc caggaatcaa
      901 cgggcgcgtc ctggcggaac gcctggcccc gcgacatccc gcgatgaagg tcctttatat
      961 gtccggttac acagacagct ttatcgccgg tcatggggtc cttgagccgg gcacgcattt
     1021 actccataaa cctttcacgg aggacgtcct tgtgcgcaaa gtgcgcgagc tattggatga
     1081 gggcagagac acgaagaaaa cagacagtgt ggaaggaacg gaatctccgg tgggagctgg
     1141 atctgacatc aaactgaaaa gataggagag catcgtacca tgcataaatt gttgattgtt
     1201 gacgacgagg agccgatgcg gcgtctgcta cgcatcaatc ttgaagaccg ctatgagatt
     1261 gtcgatacgg
//
LOCUS       DATMKH010000175         1057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4252296, whole genome shotgun sequence.
ACCESSION   DATMKH010000175 DATMKH010000000
VERSION     DATMKH010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4252296"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..>1057)
                     /locus_tag="VNV63_02150"
     CDS             complement(94..>1057)
                     /locus_tag="VNV63_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012462437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXC61454.1"
                     /translation="TFIVLLSVPFSLVGAFWMLYLLHYNMSVAVWVGLIALAGLDAET
                     GVVMLLYLDHAWEKFKAQGRMNRIEDLYAAVIEGAVQRIRPKIMTVCAIIFGLLPIMW
                     SPVYQAGADVMKRIATPMIGGVITSAILNLLIYPVIYVIWRKRSLPAEPPGGSFDGSA
                     VPESRTLASGSRRRVLTGSMAFLLTGIVIASAYFLWSSGLANSIFKSADPKMPVAEVT
                     QDGVRVSILSADGQLHAKDNALQIKFQDPGGHPVDAENVKLELNMNMPGMVMHSGAKI
                     KKDDGMGDYKADLTADMAGDWSVDLSYQGPQGPAKLKVPVNVKQ"
BASE COUNT          265 a          294 c          259 g          239 t
ORIGIN      
        1 aaagaccctt ccgcagttca ttgtgcagct tttatttacg tcagccactg tagggcaaag
       61 tggaaattct ccacggacta aatccaggac gtcttactgc ttaacgttga ccgggacttt
      121 tagcttcgcg ggtccttggg gtccctggta ggaaagatcg acgctccaat cacccgccat
      181 atccgccgtg aggtctgcct tgtaatcccc catcccatcg tcctttttta ttttcgcccc
      241 tgaatgcatc accattccgg gcatgttcat gttcagttcc agtttcacgt tctccgcgtc
      301 gacgggatgc ccacctggat cttgaaactt gatctgcaaa gcgttgtctt tggcgtgtaa
      361 ctgaccatcg gcgctcagaa tggaaaccct cacaccgtct tgagttacct cggcgacagg
      421 catctttgga tccgccgatt taaatatcga atttgctagt ccagaggacc ataggaagta
      481 ggcgcttgcg atcacgattc cagtgagcaa aaaggccatg gaaccagtaa gaacccggcg
      541 tcgagaacca gaagccagcg ttctggattc aggaacggcg gatccgtcaa aggacccacc
      601 gggaggctcc gccggaagtg aacgctttct ccaaatcacg tagatcaccg ggtagattaa
      661 tagattgaga atcgccgacg taattacgcc gccgatcatc ggagtagcaa ttcgcttcat
      721 cacatcggca ccggcttgat acactggcga ccacataatc ggtagaagtc cgaagatgat
      781 ggcgcacacg gtcatgatct ttggccgaat tcgctggacc gcaccttcga taacggcagc
      841 gtacagatct tcgatccggt tcatgcgtcc ttgtgcctta aatttttccc aggcgtggtc
      901 cagataaagg agcatgacaa ctccggtctc ggcatcaaga ccggcaagag caatcaggcc
      961 aacccacacg gcaacgctca tattgtaatg caggaggtac aacatccaga atgcccccac
     1021 caacgagaag ggtaccgaca aaaggacgat aaaggtc
//
LOCUS       DATMKH010000176         4172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4252757, whole genome shotgun sequence.
ACCESSION   DATMKH010000176 DATMKH010000000
VERSION     DATMKH010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4172
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4252757"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="VNV63_02155"
     CDS             complement(<1..356)
                     /locus_tag="VNV63_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HXC61455.1"
                     /translation="MSKGWYVVHTYSGFEGRVKASIIERVSSLGLSEKITQVLVPTEN
                     VVELREGKKKVVSKKFFPGYVLVEMELTDETSQLIKNTPKVTGFVGGGEKPAPLSVEE
                     VETLLKQLDAGTATPR"
     gene            complement(374..568)
                     /gene="secE"
                     /locus_tag="VNV63_02160"
     CDS             complement(374..568)
                     /gene="secE"
                     /locus_tag="VNV63_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013885648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HXC61456.1"
                     /translation="MKQFFQKIAEFFREVQSELKKVTYPSWPETLGSTAVVIIFVLIV
                     GVFLTLVDLVLVRAISLVIE"
     gene            complement(585..661)
                     /locus_tag="VNV63_02165"
     tRNA            complement(585..661)
                     /locus_tag="VNV63_02165"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(625..627),aa:Trp,seq:cca)
     gene            complement(660..809)
                     /gene="rpmG"
                     /locus_tag="VNV63_02170"
     CDS             complement(660..809)
                     /gene="rpmG"
                     /locus_tag="VNV63_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013913658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HXC61457.1"
                     /translation="MREIITLACSGCKHRNYSTMKNKKNDPDRLERKKYCKFCRKHTP
                     HKEVK"
     gene            complement(825..2015)
                     /gene="tuf"
                     /locus_tag="VNV63_02175"
     CDS             complement(825..2015)
                     /gene="tuf"
                     /locus_tag="VNV63_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HXC61458.1"
                     /translation="MSKGKFERTKPHVNIGTIGHVDHGKTTLTSAITKVLAKQKLAEY
                     LAYDQIDKAPEEKERGITIAIAHVEYATANRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLARQVGVPFIVVYLNKADQVTDKELLELVELEVRELLKK
                     YEFPGDDIPFITGSALKALEGDAGPLGEPSIIKLMESVDSYIPTPKRETDKPFLMPIE
                     DVFSISGRGTVVTGRVERGIVKVGDEIEIVGIRPTQKTVVTGVEMFRKLLDQGQAGDN
                     IGVLLRGTKKEDVERGMVLAKPGGITPHTKFIAESYILTKEEGGRHTPFFNGYRPQFY
                     FRTTDVTGVSKLPSGVEMVMPGDNVRLEVELITPIAMDEGLRFAIREGGKTVGAGVIS
                     KILA"
     gene            complement(2045..4126)
                     /gene="fusA"
                     /locus_tag="VNV63_02180"
     CDS             complement(2045..4126)
                     /gene="fusA"
                     /locus_tag="VNV63_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HXC61459.1"
                     /translation="MPRIYSLERTRNIGIMAHIDAGKTTTTERILFYTGITHRIGEVD
                     EGTTTMDWMVQEKERGITITSAATTCFWKDYRINIIDTPGHVDFTIEVERSLRVLDGA
                     IAVFDSVQGVEPQSETVWRQADKYHVPRIAFMNKMDRTGADFFESVKSMVDRLGVRPV
                     PIQLPIGKEGGFRGSIDLIQMKAIYFDDETLGAKFVVDEISADLLPLAQEYRQKMIEA
                     VAEFDEQAMEKYINGQTPTPAEIKLAIRNGTNLLKITPVLCGASFKNKGVQLLLDAVI
                     DFLPSPLDVPPVKGVDPKTGEELTRYPKDDDTFSALGFKIMTDPFVGQLTYFRVYSGM
                     LKAGSYVYNSTKDTKERIGRILKMHANKREDIKEVYAGEIAAAVGLRSTTTGDTLCDE
                     EHPILLEVMRFPEPVISMAIEPKTKQDQEKLGLSLQKLMREDPSFRVNTDEETGQTII
                     SGMGELHLEIIVDRLLREFTVDANVGKPQVAYKETIRSKAEGEGKYIRQTGGRGQYGH
                     VYLEVEPQEPGKGFEFVNDIVGGSIPKEYIPAVEKGIREAMVNGVLAGYPMVDFKVIL
                     TDGSYHEVDSSEMAFKIAGSMGFKEASKKASPTLLEPIMSLEVVVPEEYMGDVIGDLN
                     SRRGKIMGMKVRAAAQVIDAQVPLKEMFGYATDLRSMSQGRAVFTMQFAHYEAVPKNI
                     ADEIIAKVQGA"
BASE COUNT          918 a         1310 c          884 g         1060 t
ORIGIN      
        1 tctctcggtg ttgccgttcc ggcgtcaagc tgtttgagga gtgtttcgac ctcttccaca
       61 gacaatgggg ctggtttttc tccccctcct acgaaacctg tgaccttcgg ggtatttttg
      121 atcagctgcg aggtttcgtc cgtcagttcc atctcaacga gcacgtagcc gggaaagaat
      181 tttttgctca ccaccttctt tttgccttcc cgcaactcca ccacattctc ggtaggaacg
      241 agtacctgag taatcttttc cgataaaccc agcgagctga cacgctctat aatacttgcc
      301 ttgactcggc cttcaaaacc ggaataagta tggacaacat accagccttt tgacatacaa
      361 gcctctttat tggtcattca atcaccaaac taatcgctcg aaccagcaca agatctacta
      421 gagtaagaaa aacgcctaca atcaacacaa agataatcac cacagcggta gaaccgagag
      481 tctccggcca ggaaggataa gtgacttttt ttaattcgga ctgaacttcc ctgaaaaact
      541 cagcgatttt ttgaaagaat tgtttcactc ttgatcctta agtctggcag gccaggaggg
      601 attcgaaccc ccaacccgcg gttttggaga ccgctgctct agccgttgga gctactggcc
      661 tacttcactt ctttatgagg cgtgtgcttc cgacagaatt tgcagtattt tttccgctcc
      721 aaacggtctg ggtcgttttt cttatttttc atcgtagagt aattacgatg tttacagccg
      781 ctgcatgcta aggtaataat ctcgcgcatt ctacccgctc ccgtttacgc caagatcttg
      841 ctaatgacgc ctgcacccac cgtcttccca ccttctcgta tcgcaaaccg taatccttca
      901 tccatcgcaa tcggcgtgat tagctccact tccaaccgca cattgtctcc cggcatcacc
      961 atctccactc cggacggcaa cttcgacact cccgtcacat cggtcgtccg aaagtaaaac
     1021 tgcggccggt atccattgaa aaaaggcgtg tgccggcccc cctcttcctt cgtcagaata
     1081 tacgactccg cgataaactt cgtgtgcggc gtgatgcctc cgggcttcgc caacaccatc
     1141 cctcgctcca cgtcttcttt cttcgtccca cgcagcagca ctccgatgtt atctcccgcc
     1201 tgcccctgat ccagcagctt ccggaacatc tccacccccg tcacaaccgt tttctgcgtc
     1261 ggccgaatgc ccacaatctc gatctcgtcc cctactttca caatccctcg ctccactcgc
     1321 ccagtcacaa ccgtccctcg gcctgagatg ctgaacacgt cctcgatcgg catcaaaaat
     1381 ggcttgtccg tctctcgctt cggcgtcggg atataactat ccaccgattc catcagcttg
     1441 atgatcgacg gctcgcccag cggtcccgcg tctccctcca gggccttcag ggcactcccc
     1501 gttataaacg gaatatcatc tccaggaaac tcatacttct tcagtaactc ccgtacctca
     1561 agctccacta attccaataa ctccttgtcc gtaacctgat ccgccttgtt taaatacact
     1621 acgataaacg gcacccccac ctgacgcgcc agcaagatat gctcccgcgt ctgcggcatc
     1681 ggcccatccg ccgcgctcac caccaggata gctccatcca tctgcgccgc tcccgtgatc
     1741 atgttcttca catagtccgc atgcccagga cagtccacat gcgcatagtg ccgattggcc
     1801 gtcgcgtact ccacgtgcgc gatcgcgatc gtgatccctc gctccttctc ctccggagcc
     1861 ttgtcgatct gatcgtacgc caaatactcc gccaacttct gcttcgccaa caccttcgtg
     1921 atcgcgcttg tcagcgtcgt cttcccgtga tccacatgcc cgatcgtccc gatgtttacg
     1981 tgcggcttcg tccgctcaaa tttgcccttc gacatctctt tgcctccgtc aaaattagaa
     2041 ctacttatgc cccttgtacc tttgcaataa tctcatccgc aatgtttttg ggtacagcct
     2101 cataatgcgc gaactgcatg gtaaaaaccg cccggccctg tgacatcgaa cgcagatcgg
     2161 tcgcataacc aaacatctct tttaagggca cctgggcatc aatcacctgt gcggctgcac
     2221 gtaccttcat ccccatgatc ttgcctcggc gagaattcag gtcgccgatg acatccccca
     2281 tgtactcctc cggaacaacg acctccagag acatgatcgg ctcaagcaag gtagggctcg
     2341 cttttttaga ggcttcttta aaacccatcg agcccgcaat tttgaaggcc atctcggaag
     2401 agtctacttc atgataggat ccgtccgtaa ggatcacttt aaaatccacc ataggataac
     2461 ctgcgaggac cccgttcacc attgcttccc gaatcccttt ttccacagcc gggatgtatt
     2521 ctttcggaat cgaaccgccc acaatgtcat tgacaaactc aaaacccttg ccgggctcct
     2581 ggggctctac ctccaaataa acatgaccat actgaccccg cccgccggtt tgccggatat
     2641 actttccctc gccctccgcc ttcgatcgaa tggtttcctt gtaggccacc tgaggttttc
     2701 ccacattcgc atccactgta aactctcgga gcagccgatc cacgattatt tcgaggtgaa
     2761 gctcgcccat tcccgagata atcgtctggc cggtttcctc atccgtgttc acacggaaag
     2821 aagggtcttc gcgcatcagc ttctgcagcg agaggccgag cttttcctgg tcttgtttgg
     2881 tcttgggctc aatagccatc gagatgaccg gctctggaaa acgcatcacc tccagcagaa
     2941 tcggatgttc ttcatcgcac agggtgtctc cggtcgtggt tgatcggagc ccgactgcgg
     3001 ccgcaatctc gcccgcatat acctctttga tgtcctcccg cttgttcgca tgcatcttga
     3061 gaattcgccc aatacgctct ttggtgtctt tggtcgaatt gtaaacgtag gaaccggcct
     3121 tgagcatccc ggaatagaca cgaaaatagg tcaactgccc gacaaaagga tccgtcatga
     3181 tcttaaaacc cagggcggaa aaggtatcgt catctttcgg gtaccgggtc agttcttcgc
     3241 ctgtttttgg atccacgcct ttcaccggtg ggacgtccag cggagatggc aggaaatcaa
     3301 tcacggcatc caacagaagt tgaaccccct tgttcttaaa agaggcaccg cagagcaccg
     3361 gcgtgatctt caacaggttg gtaccgttac gtatcgccag cttgatttcc gccggggtcg
     3421 gcgtctgacc attgatgtat ttttccattg cctgctcatc aaactccgca accgcctcaa
     3481 tcatcttctg ccgatattct tgggcaagcg gcaggaggtc tgctgaaatt tcatccacca
     3541 cgaatttggc cccgagtgtc tcgtcatcaa aatagatggc tttcatctgg atcaggtcaa
     3601 tcgagccccg aaagccgccc tcttttccaa tcggcagctg aatcgggaca ggcctgacgc
     3661 ccaggcggtc caccatggac ttcacgctct cgaagaaatc ggctccggtc cggtccatct
     3721 tgttcataaa cgcaatccga gggacatgat acttatccgc ctgccgccaa accgtctcag
     3781 actggggttc aaccccttga accgaatcaa aaactgcaat cgcgccgtct aaaacacgga
     3841 gagatcgttc cacctcgatt gtaaaatcca catggcccgg cgtatcaata atattgatcc
     3901 gataatcttt ccaaaaacag gttgtcgcgg cagaggtgat ggtaatacca cgctcctttt
     3961 cctgcaccat ccagtccatc gtggtcgtac cctcgtccac ttcacctatt cgatgcgtga
     4021 tccccgtgta aaacaggatc cgctcggtgg ttgtggtctt cccggcgtca atatgagcca
     4081 tgatcccgat gtttcgtgtt ctctctaatg aatatattct tggcatttta ccaccgataa
     4141 tgcgcaaagg ccttgttggc ctccgccatc cg
//
LOCUS       DATMKH010000177         1048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4252809, whole genome shotgun sequence.
ACCESSION   DATMKH010000177 DATMKH010000000
VERSION     DATMKH010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4252809"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VNV63_02185"
     CDS             complement(<1..185)
                     /locus_tag="VNV63_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61460.1"
                     /translation="MAISFATDIRPLFRDEDIDCMKSMGIDLGNPVWMCVPANAQDVY
                     RTVSDGSMPPDEPWPADR"
     gene            complement(376..597)
                     /locus_tag="VNV63_02190"
     CDS             complement(376..597)
                     /locus_tag="VNV63_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61461.1"
                     /translation="MLDISGALNLLKAGHSIERPGPDMKGTSYFVKNENGKEYLYVRQ
                     RRLNSSEVIEEPTAFDWDDLTAKTWQAVE"
BASE COUNT          268 a          335 c          231 g          214 t
ORIGIN      
        1 cgatccgccg gccacggttc atccggaggc atcgacccat cggaaacggt ccggtaaaca
       61 tcctgcgcgt tagcaggcac acacatccag acaggattac caagatcaat ccccatcgac
      121 ttcatgcagt cgatgtcctc atcccggaat agagggcgaa tatcagtcgc aaagctaata
      181 gccattgagc ctcctttgac gagcctgggt tcaaccatgc cacatccagc gaagccagat
      241 cattcagcgc tccgcaccct cggcacgaga ccatccaatc cgggacacca tcgaaggatg
      301 gagaccaacc gggtcccagc gaatcagcaa aacaatggaa aggctaagac atcatttgct
      361 gccgagtcgt cgtctctact ctaccgcttg ccaagtcttc gccgtaaggt cgtcccagtc
      421 aaaggccgta ggctcctcga taacctcaga tgagttgagc cgcctctgtc gaacatacag
      481 gtattccttt ccattttcgt tcttcacgaa gtaggaagtg cccttcatgt ccgggccggg
      541 tcgttcgata ctgtgacctg cttttagcaa attcaaggca ccgcttatat cgagcattgc
      601 agtctccata agaattcagt tttgcccaac aggatccctg gttgatttcc tgaacatagc
      661 atgttttcgg gcgagcggcc cacctccaaa gaaaaacgtt agcgggtcag ttggaatggc
      721 agcccttgct ctcggacatc gaccgctcgc cgacccaacc aacgactccg ccaagactcc
      781 ttcaccatct ccaccaaaac gctccgcgtc tgaaaataat ctcctacccg tcaaatccat
      841 ccccgacgcc tggccgtact caatccaacc gggagagtct cccacggccc cttccacacc
      901 aggtcagcaa caaccgatga ctttccggga atcgaaagag gagacgcaac gcaatgaata
      961 caccgaatga aatcgaaatc atcccttcgc cagaagacag ccagtcaggc tcctccagac
     1021 gatcgtttct caaaggcgct gctcttac
//
LOCUS       DATMKH010000178         1006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4563273, whole genome shotgun sequence.
ACCESSION   DATMKH010000178 DATMKH010000000
VERSION     DATMKH010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4563273"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..748
                     /locus_tag="VNV63_02195"
     CDS             <1..748
                     /locus_tag="VNV63_02195"
                     /inference="COORDINATES: protein motif:HMM:NF012452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61462.1"
                     /translation="QVRKVRPCRRNFMTLAAAFRGPKGGVLLCSDQEWNDDSGSKREI
                     NKNYRINLPQCSFFISGSGPGSSVIVAWDEIHRRLWDASNAGKDLLSEHREILREAVL
                     EVFNQYDADFSYSPLSLLIMVAPRQPDRVPMMYYTEGKALIPAPHYHAVGAGKQIADY
                     FADRLYDPDRMDKKTLVALAAFILREVSASSSGVGMGANMVFINEGEQAMHYITPGII
                     KEMQDGIPSLSDAVLSYWPGRLNFPEWYGS"
     gene            complement(745..>1006)
                     /locus_tag="VNV63_02200"
     CDS             complement(745..>1006)
                     /locus_tag="VNV63_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61463.1"
                     /translation="GRDVDTLRCPHQIAAHWADYTVIRGAEAAELPNKHGAFYSVRLA
                     LRVHALRTQVHVSSLVVRLGVTQRYVAVYPKEVFKANQALYV"
BASE COUNT          249 a          244 c          274 g          239 t
ORIGIN      
        1 acaagttcgt aaagttcgtc cgtgcaggag aaacttcatg acgctggccg ctgcatttcg
       61 gggtccgaaa ggtggtgtat tgctttgttc cgatcaggag tggaatgacg attccggatc
      121 gaagcgggag atcaataaaa attatcggat caacctgccg caatgctcat tctttatctc
      181 gggatcggga cctggatcat ctgtgattgt ggcgtgggat gagatacaca gacgtttgtg
      241 ggatgcctca aatgctggga aggatttgct atccgagcac agggagatac tccgggaggc
      301 tgttttagaa gtcttcaatc aatacgatgc ggatttctct tattctccgc tatcgttact
      361 tatcatggtt gcgccgcgac agcctgatag ggtcccaatg atgtactaca cagagggaaa
      421 agctctcatt cccgctcccc actatcacgc agttggagcc ggtaagcaga tagccgatta
      481 ttttgcggat cgactttatg atccggatcg catggacaaa aaaactctcg ttgccctcgc
      541 agcgttcatt cttcgggagg tatcggcatc gtcctctggc gtcggcatgg gagcaaacat
      601 ggtgttcatc aacgaaggtg aacaggccat gcactacatc actccaggaa tcatcaagga
      661 aatgcaggat ggaattccaa gcctatccga tgcggtacta tcttattggc ccggacgctt
      721 gaattttcct gagtggtacg gaagctagac atacagcgcc tggttcgctt taaagacctc
      781 tttgggatat acagccacgt agcgctgcgt aacaccgagg cgtacaacaa gcgaagagac
      841 atgaacctgc gtgcgaagtg cgtgaacccg aagcgccaaa cgaacggaat agaacgctcc
      901 atgcttgttg ggcagttcag cggcttcggc gccccggatg accgtgtaat ctgcccagtg
      961 tgcggcgatc tgatgcggac aacgaagagt atcaacgtct cgacca
//
LOCUS       DATMKH010000179         1333 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4253101, whole genome shotgun sequence.
ACCESSION   DATMKH010000179 DATMKH010000000
VERSION     DATMKH010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1333
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4253101"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1333
                     /locus_tag="VNV63_02205"
     CDS             <1..>1333
                     /locus_tag="VNV63_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXC61464.1"
                     /translation="LPRSQRSPALRDAIQTFQPDLLLFSWRDIQIFAPHDGDPTLKYA
                     FTMDYSRNPIKRLVASLKGLRSFIAYHQGIRECLSYPWLVRSEFPQLQMMIGGGAFSV
                     FSDQLIQMLPEGIIGILGEGEGTVLKMIEGRNLSEERYITRQNGRVLEGKKSSTRDIS
                     SLMMDLAYLETVFPQHAAYHDECIGVQTKRGCPYDCQFCEYPYIEGKRVRYRPPEKIL
                     ADILQHYQRWGTRRFWFTDAQFITGKQALPQCTEILERIIASGLKIEWSGYIRTSLIT
                     APLAKLMVQSGVGDLEVAITSGSQEVLNELHMGFKLEHLYEGCRYLKEAGFNGRVLLN
                     YSLNSPGDTEETLRESVESYKKIAEILGENRVFPMLFFLGIQPHTGLEDRLIGEGYLS
                     SGYNPLSMNPFLIKKLLYNPAPLSHRIAKACLSAWEITARSKSAETGIPMKT"
BASE COUNT          296 a          384 c          353 g          300 t
ORIGIN      
        1 ttctcccgcg gtcccaacgc tccccggccc tgcgtgatgc gatccagacc ttccagcccg
       61 accttctcct cttctcatgg cgggatatcc agattttcgc cccccacgac ggcgacccga
      121 cgctcaaata cgccttcacg atggactact ctcgaaatcc gatcaagcgt ctagtcgctt
      181 cgcttaaagg ccttcgatct tttatcgctt accatcaagg catccgcgaa tgcctctcct
      241 atccctggct tgttcgatcc gagtttcctc agcttcagat gatgatcggc ggcggggcgt
      301 tcagcgtctt ttcggatcaa ctcattcaga tgctcccgga agggattatc ggcatactgg
      361 gcgaaggcga ggggacggtg ctcaagatga tcgagggacg gaatctcagc gaggaacgtt
      421 acatcacacg gcagaatggt cgagttctcg aaggaaagaa gagcagcacg cgtgatattt
      481 ccagtctgat gatggatctt gcctatttag aaaccgtctt ccctcagcat gcggcctatc
      541 atgacgagtg catcggggtt cagacaaagc gcggctgccc gtatgactgc cagttctgcg
      601 aataccctta tatcgaggga aaacgcgtcc ggtaccggcc gccggagaag atcctggccg
      661 acattcttca gcattatcag cgctggggaa cccgccgctt ctggttcacg gatgcacaat
      721 ttatcaccgg gaaacaagcc ttgccgcaat gcactgagat cctggaacgg atcatcgcaa
      781 gcgggctgaa gatcgaatgg agcggttata tccggaccag cctgatcacg gcgcccttgg
      841 caaaactcat ggttcagtcc ggcgtcggtg accttgaggt cgcaattacg tccggatcgc
      901 aggaggtctt aaacgagctc catatgggat ttaaactcga gcatctctac gaaggttgcc
      961 ggtaccttaa agaggccggc tttaacggaa gggttcttct caactactcg ctcaactcgc
     1021 cgggagatac cgaggagacg cttcgagaga gcgtcgagtc ttacaagaag attgccgaga
     1081 tcttgggaga gaaccgggtt tttccgatgc tgttcttcct cggcatccag cctcacaccg
     1141 gcctggagga tcggctgatc ggcgagggat atctctcttc cggctacaat ccgctctcga
     1201 tgaatccgtt tctcatcaag aagcttttgt ataatccggc tcctttaagc caccggatcg
     1261 caaaggcctg tctttccgcc tgggagatca cggcacgcag caaatcagcc gagaccggca
     1321 tcccgatgaa gac
//
LOCUS       DATMKH010000180         3017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3919298, whole genome shotgun sequence.
ACCESSION   DATMKH010000180 DATMKH010000000
VERSION     DATMKH010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3919298"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      335..454
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            549..2438
                     /gene="thiC"
                     /locus_tag="VNV63_02210"
     CDS             549..2438
                     /gene="thiC"
                     /locus_tag="VNV63_02210"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009583617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HXC61465.1"
                     /translation="MATQTNGTKGEILTTQPLPASRKTYIAGKQPGVRVAMQEVQLTP
                     THIMTDGSVQENPPLVVYDPSGPYTDPNITIDIRNGLTPLRSSWIHEREDVEKLHDIS
                     SDYGRRRASDPKLSDLRFQHIQKPLRAKHGMNATQMHYARKGIITPEMEYIAIRENQM
                     RALAKEPKYLTEGPAGKGVAQHPGNSWGASIPSEITPEFVRDEVARGRAIIPVNINHP
                     ESEPMIIGRNFLVKINANIGNSAVASSIEEEVEKMIWAIRWGADTVMDLSTGKNIHET
                     REWIIRNSPVPIGTVPIYQALEKVGGKAEELTWEIFHDTLIEQAEQGVDYFTIHAGVL
                     LRYVPLTAKRLTGIVSRGGSILAKWCLAHHQENFLYTHFEEICEIMKAYDVSFSLGDG
                     LRPGSIADANDEAQVGELETLGKLTQIAWRHDVQTMIEGPGHVPMHLIQVNMEKQLKE
                     CHEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWFGCAMLCYVTPKEHLGLPTKEDVK
                     QGVIAYKIAAHAADLAKGHPGAQSRDNALSAARFEFRWEDQFNLSLDPETAKEFHDET
                     LPAEGAKVAHFCSMCGPHFCSMKITEDVREYAKQKEIDERAALEIGMKEKSQEFKKAG
                     SQIYQ"
     gene            2461..>3017
                     /locus_tag="VNV63_02215"
     CDS             2461..>3017
                     /locus_tag="VNV63_02215"
                     /EC_number="2.4.2.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide-dependent adenosine diphosphate thiazole
                     synthase"
                     /protein_id="HXC61466.1"
                     /translation="MKEQDITPLRERDITKSIAREFYQEFDQLIESDVIIVGAGPAGL
                     VCARDLASSGHKVLLIEQMAHLGGGFWSGGYLMTKAAIASPAHEMLLKLNVPCKETGK
                     GLYIVDAAHACAKLIGAALEAGAKLLNLTKVVDLIVREKNRVEGVVVNWAPVEAMGHD
                     IAHVDPIALESKIVVDATGHDAVTL"
BASE COUNT          776 a          794 c          813 g          634 t
ORIGIN      
        1 catccccggt ccttcgagca cctttgccat ctaacatacc tccaaatttt gtagcagact
       61 aagacgtggc cagttgatcc attagtgatt ttaaataaaa acccaagaaa aataggtgtt
      121 atgctgttta acgacacaaa acaagtggct tattgtagcc tgatcttttg aattcatcaa
      181 tcaaaaaaaa ggcctagtcc gggtcaagac gggaataggt cttaagaccc agtttcttct
      241 gttttagtct gtcaggataa agtcaagacg cactctcatg tccagccata aaatagttga
      301 cacctgctgt tttcccttgt attatgaatc cactgtttat cgctagggga gtcccgtgaa
      361 acggggctga gagatccgat cgattaagaa tcggagcaga ccctctgaac ctgacctgga
      421 tcatcccagc gaagggaagc gcatccaaat tagaattctt ggaaaccgca cccttatctg
      481 gtgcggtttt ttagtttgga ggcaagagcc tttagaaaca agagcctttg gagaaaggac
      541 ataggattat ggcgacacag accaacggaa caaaggggga aatcttgacc acccaacctc
      601 ttcccgcatc aaggaagacg tatattgccg gaaagcaacc gggcgttcgt gttgcgatgc
      661 aggaggttca acttacgccc acacacatca tgacggatgg ttcagttcaa gaaaatccgc
      721 cgttggtcgt ctacgatccc tccggtccct atacagatcc caatattacg attgacatcc
      781 gcaacggcct cacgccccta cggtcttcat ggattcatga gcgagaggac gtcgaaaaac
      841 tccacgacat ttcatccgat tacggccgca ggcgggcttc ggatcccaag ctcagcgatc
      901 tccggtttca gcacatccaa aagcctttgc gcgcaaaaca tggtatgaac gccacccaaa
      961 tgcattatgc ccgtaaaggg atcattacgc cggagatgga atatatcgcc atacgcgaaa
     1021 atcagatgcg tgcactggcc aaggagccca agtatctcac ggaggggccc gctgggaagg
     1081 gagtcgcaca acatccgggg aattcatggg gggcttctat cccatccgag atcactccgg
     1141 aattcgtccg tgatgaagtc gcgaggggaa gagccattat tcctgtaaac atcaaccacc
     1201 ccgaaagcga gccgatgatt atcggccgca attttctggt aaagatcaat gcgaatatcg
     1261 gcaactccgc cgttgcctcc tcgatcgaag aggaggtaga aaaaatgatc tgggccatac
     1321 gttggggtgc cgacaccgtg atggacctct ccacgggtaa aaacatccac gagacgcggg
     1381 aatggatcat tcgcaactcg ccggtaccca tcggcaccgt gccgatctac caagcgcttg
     1441 agaaggtcgg cggcaaggcg gaggaactga cctgggagat cttccacgat acgttgatcg
     1501 aacaggcaga acagggtgtg gactacttca cgatccatgc cggcgtgctg ctgcgttatg
     1561 tcccgctcac cgccaagcgc ttgaccggaa tcgtatcgcg cggcggctcg attttggcaa
     1621 aatggtgtct tgcgcaccat caggagaact tcctctacac ccattttgaa gaaatctgcg
     1681 agatcatgaa agcatacgac gtctcgttca gcctgggcga cggccttcgg cccggctcta
     1741 tcgcggatgc gaacgatgag gcccaggttg gcgagcttga gacgctgggc aagctgacac
     1801 aaatcgcctg gaggcatgat gttcagacga tgatcgaagg accgggtcat gtccccatgc
     1861 atctgattca agtgaacatg gagaaacagc ttaaggaatg tcatgaggcg ccgttctaca
     1921 ccttgggccc cctgaccacc gatatcgccc ccggctatga ccacatcacc tcggccatcg
     1981 gcgctgcgat gatcggctgg ttcggctgcg cgatgctttg ctacgtaacg ccgaaagagc
     2041 atcttggttt gccgaccaaa gaagacgtca aacaaggagt catcgcctac aagattgcgg
     2101 cccatgcagc ggatctggcc aagggacatc cgggcgctca gtcccgcgac aatgcgctat
     2161 cggccgcccg ctttgaattc cggtgggaag accagttcaa tctctccctg gatccggaaa
     2221 ccgcaaaaga gtttcacgac gagacactgc cggcagaggg cgcaaaagtc gcgcacttct
     2281 gttccatgtg cggcccgcat ttctgctcga tgaagattac ggaggacgtc cgcgaatacg
     2341 ccaaacagaa ggagatcgac gagcgggcgg cgcttgaaat cggaatgaag gaaaaatcgc
     2401 aggagtttaa aaaagccgga tcacagattt atcaatgacg aatttgtttc ggagatttcc
     2461 atgaaagaac aagacatcac cccgcttcgt gagcgggata tcacgaagag catcgcacgt
     2521 gaattctatc aggagtttga ccagctcatt gagagtgacg tgattatcgt cggggccggg
     2581 ccggcaggtc tggtctgcgc gcgcgatctc gcaagcagcg gccataaagt tctcctaatc
     2641 gagcagatgg cccacctcgg aggcggcttc tggtccggcg gttatctcat gaccaaggcc
     2701 gcgatcgctt ctcccgccca tgaaatgctt ttaaagctca atgtcccttg taaagaaacc
     2761 ggtaaaggtc tctacatcgt ggatgcggca catgcctgcg ccaagctgat cggggctgca
     2821 ttggaagccg gagctaagct attaaacctc accaaggtgg tggacctgat tgtgcgggaa
     2881 aagaaccgag tcgagggagt cgtagtcaat tgggcaccgg tggaagcgat ggggcatgat
     2941 attgcgcatg tggacccgat cgcgctggag tccaagattg tggtggatgc gacgggacat
     3001 gacgccgtga cgctcca
//
LOCUS       DATMKH010000181         3095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_407298,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000181 DATMKH010000000
VERSION     DATMKH010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407298"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..926
                     /locus_tag="VNV63_02220"
     CDS             <1..926
                     /locus_tag="VNV63_02220"
                     /inference="COORDINATES: protein motif:HMM:NF012263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HXC61467.1"
                     /translation="SLHYWTAQAMVVCMLAHLLRVFITGAYKFPRILTWYTGVALFFT
                     ITMLSFFSGTVIKWDQEGYEALHHYKAVVNLLGPLGALLGEGLTESISLNVRMYTFHI
                     TLAPLLLIFLILCHFYLIHVFNISPLPSGPSGRLPELPAAELTSTFTEDIKSIIRSSL
                     IFYGLVAVLSAFVPAPLGGEHSPVFTGAKPPWVFLWQYGIEHFTEIPGILYSSLLLLL
                     IFLIVPWIDRGPSRNPRERKGVLALGTITALVLIGFTVFAWVAPPEVHHEGGGHHMED
                     MPGMGGGKETPSGEHSDEHSHDDTAAPGTNR"
     gene            1042..1926
                     /locus_tag="VNV63_02225"
     CDS             1042..1926
                     /locus_tag="VNV63_02225"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HXC61468.1"
                     /translation="MAQVEEARLRRHLENICFLRSQVLNPGAVKRTAHYIEQELRSYG
                     LEIDADGFYHWFTTWHRNKNIIGGFPHESQKAPCLIIGAHYDAVPFSPGADDNGSGVA
                     VLLEAARILSQTNHPSRLHVRFVAFAMEEYGMIGSQHYVETLQKNKTAVAGMLSLECV
                     GYTNSQPGSQIIPPGLPIQVPDRGDFIGIVGNADSGELKECLESAVHQYAPGLKAVGL
                     LVPENGRVLPATRLSDHSPFWDAGYPALLVTDTAFLRNPHYHRPSDRIETLDFSFMTL
                     LTQSIVAFVKELYQTRPN"
     gene            1977..2696
                     /locus_tag="VNV63_02230"
     CDS             1977..2696
                     /locus_tag="VNV63_02230"
                     /inference="COORDINATES: protein motif:HMM:TIGR02252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HXC61469.1"
                     /translation="MKPDQNLNSLKAVFFDAAGTLFRVRGTVGQIYWRLAQPYGLQAT
                     PEAIEQAFQEQFANAPALAFPNRTPEEVQALERRWWEKVMRLVFQEIGMFPRFNDYFN
                     EVYEVFKGTEGWELYPETLDVLTRLKSEGRYIGIISNFDSRLHEVCRALKISPYLDSV
                     TISSQTGWSKPSPEIFSQALKSRSLGSGEAVHVGDEVGDDVQGALAAGLRPVFLDRSN
                     QEQAPPGVTKISRLDELWEVL"
     gene            2700..>3095
                     /locus_tag="VNV63_02235"
     CDS             2700..>3095
                     /locus_tag="VNV63_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002645247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D ubiquinol oxidase subunit II"
                     /protein_id="HXC61470.1"
                     /translation="MTPPGDAQKTSRRKPDAKDVLDDQIISLIRQAGGTPHADLIKEI
                     IITAVKLVGDHPSYGDLKILNAALKELRYAFKLFAPYRHIRKVTVFGSARTREEEPAY
                     QQAVKFARLISQSGFMVITGAGDGIMQGAQ"
BASE COUNT          679 a          829 c          848 g          739 t
ORIGIN      
        1 gctcgcttca ttactggacg gcgcaagcca tggttgtttg tatgctcgcg catcttttaa
       61 gagtgttcat tacgggggcc tacaaatttc cgcgcatcct tacctggtac accggtgtcg
      121 cccttttctt cactataacg atgctctcct ttttttccgg caccgtcata aaatgggatc
      181 aggaagggta tgaggcgctg catcattata aggccgtggt caacttgctg gggcctttgg
      241 gtgcgttgct tggcgaaggg ctgacagaga gcatttcact caacgtccgt atgtacactt
      301 ttcatatcac actcgctcca ctgcttctga tttttttaat tctatgccac ttttatctga
      361 tccatgtttt taatatctcg ccgcttccca gcggaccatc cgggcgcctg cccgaacttc
      421 cagcggccga actgaccagc acctttacgg aagatatcaa gagcattatc cggtccagcc
      481 tgatcttcta tggcctcgtg gcagtccttt cggcgttcgt ccctgcaccg ctcggtggtg
      541 agcattctcc ggtattcacc ggagcgaagc cgccatgggt ctttctctgg cagtatggaa
      601 tcgagcattt taccgaaatt cccgggatac tttatagcag tcttctgctg ctccttattt
      661 ttctcattgt tccatggatt gatcgcgggc cgagccggaa cccgagagag cggaaaggtg
      721 tgctagccct gggaacgatc acggcgctgg tcttaatagg ttttaccgtt tttgcctggg
      781 ttgctccccc tgaggttcat cacgagggcg gggggcatca tatggaagat atgcccggaa
      841 tgggaggcgg aaaagagacg ccgtctgggg aacattctga tgaacattct catgatgaca
      901 ccgcagcgcc ggggaccaat cggtgagaag ctccccacgg aggagtccaa caaacacacc
      961 cgctgaagaa ttcggtgcat gtccctgaag tatcttgcac gcgcgaagtt tcaataagcg
     1021 gatcatcgta cagaaactgt catggcacag gtggaagagg cccggctccg gcggcatctt
     1081 gagaacatct gctttctgag aagccaggtc ttaaatccgg gagcggtgaa gcgcacggcc
     1141 cattatatcg agcaggagct ccggtcctat ggtctggaga tcgatgccga tggattttac
     1201 cattggttca ccacctggca ccgcaacaaa aatattattg gcggttttcc ccatgagagt
     1261 caaaaagctc cctgtctcat catcggcgcc cattatgacg ccgtgccgtt ctctcccggc
     1321 gcggatgaca atggcagcgg cgtggcggtg ctgctggaag cggcgcgcat cttaagccag
     1381 acaaaccatc cgtctcgcct tcatgtccgt ttcgtagctt ttgcaatgga agagtacggc
     1441 atgatcggca gccagcatta tgtagagacg cttcaaaaga acaagactgc ggttgccggc
     1501 atgttatcgc tcgaatgtgt cggttatacg aatagccagc ccggatcgca gatcatcccg
     1561 ccgggtcttc ccattcaggt tccggatcgg ggagatttta tcggcatcgt gggtaatgcc
     1621 gattccggcg aactgaaaga atgtctggag tcggccgtcc atcagtatgc ccccggactc
     1681 aaagcggttg ggcttcttgt cccggaaaac ggtcgtgttc ttccggcaac ccgcttgagc
     1741 gatcattcgc ctttctggga tgctggctac cctgcgctac tggttacgga cacagctttt
     1801 cttagaaacc cgcattatca tcgtcccagc gatcggatcg agacgctgga cttctcattt
     1861 atgaccttgc tgactcaatc catcgttgcc tttgtgaagg aactgtacca gacgcggcca
     1921 aattgagaat tagaccctac ctttacaaaa ccagggtgtt ccattacaat acctaagtga
     1981 agccggacca aaatttaaac agcctcaagg cggtcttctt tgacgctgcc gggacgctct
     2041 ttcgtgtgcg cggcacggtg ggccagatct actggcggtt ggcccaacct tacgggttac
     2101 aagcaacccc cgaggcgatt gagcaggcct tccaggaaca atttgcgaac gctccggctc
     2161 tggcttttcc gaaccggaca ccggaagagg ttcaggcttt ggagcgccga tggtgggaga
     2221 aagtgatgcg gcttgtgttc caggaaatcg ggatgtttcc cagatttaac gactatttca
     2281 atgaagtgta cgaggtcttc aaaggcacgg agggatggga gctttacccg gagacactgg
     2341 acgttctgac caggctaaaa tccgaaggac gctacatcgg tatcatctcc aacttcgatt
     2401 cccgcctcca tgaggtctgc cgggctttaa aaatttctcc ttatctcgat tcggtcacga
     2461 tctcaagcca gaccggctgg tccaaacctt cgcctgagat tttctcacag gcgctaaagt
     2521 cgcgcagcct cggttcgggt gaagcggtcc atgtcgggga cgaggtgggg gatgacgttc
     2581 aaggcgccct ggcggcgggt ctgcggccgg tgtttctcga ccggtcgaat caggagcagg
     2641 caccgcctgg cgtgactaag attagcaggt tagatgaact gtgggaggta ctttagaaga
     2701 tgacgccccc cggcgatgct caaaagacat cccgccgaaa gccggatgca aaggatgtgc
     2761 tcgatgatca gatcatatcg ctgattcgtc aagcgggagg aactccccac gccgatctga
     2821 tcaaagagat catcatcacg gccgtgaagt tagttgggga tcatccttcc tacggggatc
     2881 tcaagatcct caatgcagcg ctcaaagagc tccgatacgc attcaaactc ttcgcaccgt
     2941 atcgtcacat ccgaaaggtg acagtcttcg gctccgcgag aacgagagaa gaggagccgg
     3001 cttatcagca ggcggtgaaa tttgcccggc tgatcagcca gtcaggcttc atggtgatca
     3061 cgggagccgg agatggcatc atgcagggtg cgcag
//
LOCUS       DATMKH010000182         3258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2008813, whole genome shotgun sequence.
ACCESSION   DATMKH010000182 DATMKH010000000
VERSION     DATMKH010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2008813"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..703
                     /gene="rplC"
                     /locus_tag="VNV63_02240"
     CDS             77..703
                     /gene="rplC"
                     /locus_tag="VNV63_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HXC61471.1"
                     /translation="MINGMLGEKIGMTQIYDAQGKSIQVTVVEAGPCRVIQKKTVQLD
                     GYEAVQLAFREISEKKVSKALLGHFKKSQAPPARFCREFKADLSKVELGQMITADIFK
                     VGDWVDVSGISKGKGFAGVMKRHHFKGGPATHGSMFHREPGSIGSSSWPSRVRKNKRL
                     PGRMGSERVTTLRLEVVEVREKENLIFIRGALPGSAGALLEIRRSNRV"
     gene            715..1338
                     /gene="rplD"
                     /locus_tag="VNV63_02245"
     CDS             715..1338
                     /gene="rplD"
                     /locus_tag="VNV63_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HXC61472.1"
                     /translation="MPDTDVVDLNKKIVGKIQLDERIFKASPDLSLVHEAVVMQLASQ
                     RQGTASTKTRGEVSGGGKKPWKQKGTGRARAGSIRSPLWKGGGTIFGPRPRDYGYSIP
                     KKKYRTALRAVLSARFKEGAITVVDRLEIEGPKTKMLVQALGRLGLNKKVAILMDQAN
                     QNIERAARNLPGVKVLLIEKLNVYDLLDARQLLIAQPAVSRLVEVWS"
     gene            1335..1628
                     /gene="rplW"
                     /locus_tag="VNV63_02250"
     CDS             1335..1628
                     /gene="rplW"
                     /locus_tag="VNV63_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HXC61473.1"
                     /translation="MSKNPHDILIRPIITEKVTALREQGNKVCFFVDPRANRIEVKKA
                     VEEALKVKVEKVNIISVHGKLKRFGRFEGKKPDRKKAIVTLKAGEKLEFLEGV"
     gene            1634..2458
                     /gene="rplB"
                     /locus_tag="VNV63_02255"
     CDS             1634..2458
                     /gene="rplB"
                     /locus_tag="VNV63_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HXC61474.1"
                     /translation="MPTRQYKPTSPGRRSMTVSTFDEITKTKPEKSLVTSRPPSGGRN
                     NCGKMTVRHRGGGHKRAYRIIDFKRDKWDIPARVAAIEYDPNRSARIALLHYADGEKR
                     YIIAPEGVRVGETVMSGRSAEIKTGNALPFINIPVGTLVHNVELKRGAGGQLVRSAGG
                     VAQVMAKEGDYVHLRLASGEVRLVHVECMATIGQVGNLDHENVSIGKAGRSRWLGLRP
                     KVRGVAMNPVDHPHGGGEGKSGQGNPHPVSPWGQLAKGYKTRRRKSTTRFIIKRRK"
     gene            2573..2860
                     /gene="rpsS"
                     /locus_tag="VNV63_02260"
     CDS             2573..2860
                     /gene="rpsS"
                     /locus_tag="VNV63_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009383014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HXC61475.1"
                     /translation="MARSISKGPFVDESLAKKIDALNQANDKKLIKSWSRRSTVIPDM
                     IGHTIAVHNGRKFIPVFITENMVGHKLGEFSPTRFFKGHGAVKAEKAPPPK"
     gene            2877..>3258
                     /gene="rplV"
                     /locus_tag="VNV63_02265"
     CDS             2877..>3258
                     /gene="rplV"
                     /locus_tag="VNV63_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009383015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HXC61476.1"
                     /translation="MEARAVLKYVRMSPRKARMVVDMIRGRKVEDALNLLKFTRRRAA
                     KVVEKVLKSALANATQKEIGDVDDLRVSKAYVDGGPTEKRIQPTAMGRSWRIHKRSSH
                     ITIVLSPPVGSTQLPAGQAGKKTAA"
BASE COUNT          811 a          751 c         1020 g          676 t
ORIGIN      
        1 cgtggatgcc ctgatgaaat taaatctctc cgcaggggtg ggtgttgaaa tcaaacaata
       61 agaaggctgt cgttcgatga taaatggaat gctgggtgag aagatcggga tgacgcagat
      121 ttacgatgcg cagggtaaat cgatccaggt caccgtggtt gaggcggggc cttgccgtgt
      181 gatccaaaag aaaacggtac agcttgacgg ctatgaggcg gtccaactcg cttttcggga
      241 gatttccgag aagaaagtca gcaaagcgct tctcggccat tttaagaaga gtcaagcgcc
      301 gccggccagg ttttgccgtg agtttaaagc ggatctcagc aaggtggagc tgggacagat
      361 gattacggct gacattttta aagtcggtga ttgggtagac gtgagcggga tatcgaaagg
      421 taaggggttc gccggtgtga tgaagcgtca ccattttaag ggaggacccg cgacccatgg
      481 ttcgatgttc catcgcgagc ccggttcgat cggcagcagt tcctggccgt cccgcgtccg
      541 aaaaaataag cggttgccgg gcagaatggg ctcggagcga gtgacaacgc tgcgtcttga
      601 ggtggtcgag gttcgagaaa aggaaaatct aatcttcatt cggggcgcat tgccaggcag
      661 tgctggcgcc ctcctggaga tccgtcggtc aaacagagtt taggatatca tttcatgccg
      721 gacacggacg tggtagatct caataagaag attgttggaa agatccagtt ggatgaacgg
      781 atctttaagg catctcccga tctctctcta gttcacgagg cggttgtcat gcagcttgcc
      841 tcgcagcgcc agggtaccgc ctcgaccaag actcggggag aggtgagcgg aggcggtaaa
      901 aaaccttgga aacagaaggg aaccgggagg gcgcgggccg gttcgatccg gtctccacta
      961 tggaaaggcg gcggaaccat tttcggtccc cgtccccgag actacggcta ttcgattccc
     1021 aagaaaaagt accggaccgc tctgagagct gttttgtccg cccgatttaa agagggcgcc
     1081 atcactgtgg tggatcggct ggagatcgaa ggaccgaaaa cgaagatgct cgttcaggct
     1141 ctgggacgtt tgggactcaa taaaaaagtg gcgattctga tggatcaggc caaccagaat
     1201 attgaacggg ccgcaaggaa tcttcccgga gtcaaggtcc tgcttatcga gaaactgaat
     1261 gtgtatgatt tgctcgatgc gaggcagctt ctcatcgcac aaccggccgt gagccgttta
     1321 gtcgaggttt ggtcatgagc aaaaatccac acgacatttt gatcagaccg atcatcacgg
     1381 agaaggttac ggcattgcgt gaacagggga acaaggtctg cttctttgtg gacccccgtg
     1441 cgaatcggat cgaggtcaag aaagcggtgg aagaggcatt gaaggtcaaa gtggaaaaag
     1501 taaatatcat ttcggtccac ggcaagctca agcgtttcgg acgttttgaa gggaaaaagc
     1561 ccgaccggaa aaaagccatc gtgaccttga aagcgggaga gaaacttgag tttcttgagg
     1621 gggtataaag cccatgccga ccagacagta taaaccaacc tcaccgggcc gccgttcaat
     1681 gacggtgtcg acctttgatg agatcacaaa gacgaagccc gagaagagtt tggtgacttc
     1741 gcgcccgccc tcgggcggaa ggaataactg cgggaagatg actgtccgcc atcggggagg
     1801 cggccacaag cgtgcctatc gcatcattga ttttaaacgg gacaaatggg atatcccggc
     1861 ccgtgtcgcg gcaatcgaat atgaccccaa tcgctcggca cggatcgcct tgcttcacta
     1921 tgccgatggc gagaagcgtt acatcatcgc tccggaagga gttcgggtcg gtgagacggt
     1981 gatgtccggt cgttcagcgg agatcaagac agggaatgct cttcccttca tcaatatccc
     2041 ggtcggcacg ctggtccaca acgtggagct gaagaggggt gcgggcggac agctcgttcg
     2101 cagcgctgga ggggtggcgc aggtgatggc caaggaaggt gattatgttc acctccggct
     2161 cgcttccggt gaggtgcgcc tggttcatgt ggaatgcatg gcgaccatag gtcaggtcgg
     2221 taatctggat catgagaatg tctcgatcgg gaaggccggg cgttcacgct ggttggggtt
     2281 gcggcccaag gtgcgtggtg tggccatgaa cccggtcgat catccccacg gtggcggaga
     2341 aggcaagtcg ggtcaaggca acccgcatcc ggtatcgccc tggggccagc tggccaaggg
     2401 atataagacg agacgccgga aatctaccac tcgctttatt attaagcgca ggaagtagat
     2461 gcccatgggt gtctgtccag cgagcaaggc gagtgagagg gggaggctct aaaggctttg
     2521 cctttagagg gggcgacgca agcccatata aatagttttt gggagagatt agatggcaag
     2581 atccattagc aagggtcctt ttgtggatga gagtttggca aagaagattg acgctctcaa
     2641 tcaggcgaac gacaagaagc ttatcaagtc ctggtcaaga agatccacgg tgatacccga
     2701 tatgattggc cacacgattg cggtacataa cggcaggaaa ttcatccctg tttttattac
     2761 ggagaacatg gtcggtcaca agctgggtga gttttcgccc acacggtttt tcaaaggtca
     2821 cggggctgtg aaagccgaga aggcaccacc accgaagtaa cggtcagagg aaattaatgg
     2881 aagcaagagc ggtattaaaa tatgtgcgta tgtcacctcg aaaggcgcgg atggtggtgg
     2941 acatgattcg cggccgcaag gtggaggacg cgcttaacct ccttaaattt acacgtcgcc
     3001 gtgcagctaa ggtcgtggag aaggtcctga aatcggctct tgccaatgcg acccagaaag
     3061 agatcgggga tgtggatgat ctgagagtga gcaaggccta tgtggatggc ggcccgactg
     3121 agaaacggat tcagcccacc gcaatgggcc ggtcctggag gatccataaa cggagtagtc
     3181 atatcacgat cgttctgtct ccaccggttg gatcaactca gttgcctgcc ggccaggcgg
     3241 ggaagaaaac cgcggcag
//
LOCUS       DATMKH010000183         1675 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1686160, whole genome shotgun sequence.
ACCESSION   DATMKH010000183 DATMKH010000000
VERSION     DATMKH010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1686160"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /locus_tag="VNV63_02270"
     CDS             <1..722
                     /locus_tag="VNV63_02270"
                     /inference="COORDINATES: protein motif:HMM:NF013234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXC61477.1"
                     /translation="QAVIQAVYQRLPSISIDHGVLSKSMRNAAILKDFGWADVGSWAA
                     LGELKERQDKNGNVVSGNVLDLESRDSVIYADQRLVATVGLEDMVVVDTADATLVCPK
                     ERAQDIKRVVEQLKKRQAKEQQIHTTVNRPWGSYTVLEEGNGYKIKRIEVKPGARLSL
                     QMHHKRSEHWVVVSGVAQVTCGDRVYQVKRNESTYVPVRMKHRIANPGKEPLQIIEVQ
                     NGGYLGEDDIVRFQDDYDRSS"
     gene            814..>1675
                     /locus_tag="VNV63_02275"
     CDS             814..>1675
                     /locus_tag="VNV63_02275"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HXC61478.1"
                     /translation="MVNPNIFREYDIRGVVEKDLTHDAVEEIGRSFGSEMMQAGHRQL
                     SLGRDGRLSSPALHDSLIQGLTSTGVEILDIGICPTPILYFSLFELPVQGGVMITGSH
                     NPADFNGFKLCRGRESIYGDAIQAIRKRIESKTFVQGKGLVKNYPGIIPQYTKYLEKQ
                     FRDAAKGFKVVVDSGNGTAGPVAPDLLRLMGCEVVELYSEMDGRFPNHHPDPTVPENL
                     TDLITRVKAEGAAMGIGFDGDADRLGVVDENGEVIWGDRLLIIFARDILRRHPKATVV
                     SEVKCSQQLFD"
BASE COUNT          409 a          382 c          506 g          378 t
ORIGIN      
        1 aacaggcggt gatccaggcg gtatatcaga ggctgccttc tatctcaatc gatcatggag
       61 tactttcaaa atctatgcgt aatgcagcga ttttaaaaga ctttggatgg gcagacgtcg
      121 gcagctgggc cgctctcgga gaactaaagg agcgtcaaga taaaaatggg aatgtcgtga
      181 gcgggaatgt gctggatctg gagagccgtg attcggtaat ctatgccgat caacgcctgg
      241 tggccacggt tggactcgaa gacatggtcg tggtggacac ggctgatgcg acactcgtgt
      301 gccccaaaga acgtgcccag gatatcaagc gcgttgttga gcagctcaag aagcggcaag
      361 cgaaggagca gcagatccat accacggtca accggccgtg gggctcatac actgttctcg
      421 aagaagggaa tggctacaag atcaaacgta ttgaggtcaa accgggtgcc cgcttgagcc
      481 ttcagatgca tcataagcgg agcgagcatt gggtggtcgt ttccggcgtt gcgcaggtga
      541 cctgcggcga ccgggtgtac caggtaaaga gaaacgagag cacctatgtt ccggtaagaa
      601 tgaaacatcg tattgccaac ccgggaaaag aaccgctaca gattatcgag gtccagaacg
      661 gaggctatct cggtgaagac gatatcgtac gattccaaga tgattatgat agaagcagtt
      721 gatgcacatt tacgttgtta tagactagac tggcaggctg tcttcttgga cagcagggag
      781 cgagagtaag catgggaagg ggaatgttcc aacgtggtta atccgaatat atttcgcgaa
      841 tatgacatcc gtggcgtggt tgaaaaagac ctcacccatg atgccgttga ggagatcggc
      901 cgctcctttg gctccgagat gatgcaggcg ggccatcggc aactgagcct cgggagagac
      961 ggtcgcctga gctctcctgc gctgcacgat tcgttgatcc aaggacttac ctctaccggt
     1021 gtggagatct tggatatcgg gatctgcccg acgccgattc tctatttctc gctctttgag
     1081 cttccggtac agggaggtgt catgattacc ggcagccaca accctgccga ctttaacggg
     1141 tttaaactct gtcggggacg agagtcaatt tatggcgatg caattcaggc gatccgtaag
     1201 cgcatcgagt cgaagacttt tgtgcagggc aaggggcttg tcaagaacta tccaggcatc
     1261 atcccgcaat atacaaaata tttggaaaaa cagttcaggg atgccgctaa aggttttaaa
     1321 gtcgttgtgg attccggcaa tggcacggcc ggtcccgttg ctccagattt gcttcgactg
     1381 atgggttgtg aggtggtcga gctctattcc gaaatggacg gccggtttcc gaaccatcac
     1441 ccggatccga cggtaccgga gaatttgact gatctgatca cacgcgtcaa agcggagggg
     1501 gccgcgatgg gaatcggttt cgatggcgat gccgaccggc tgggcgtggt ggatgagaat
     1561 ggagaggtta tctggggaga tcggctcctg attatttttg cgcgtgacat cttaagacgg
     1621 catccgaaag cgaccgtggt ctctgaggtg aaatgttcac agcagctctt tgacg
//
LOCUS       DATMKH010000184         1070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4564187, whole genome shotgun sequence.
ACCESSION   DATMKH010000184 DATMKH010000000
VERSION     DATMKH010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4564187"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..>1070)
                     /locus_tag="VNV63_02280"
     CDS             complement(53..>1070)
                     /locus_tag="VNV63_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HXC61479.1"
                     /translation="EKPTGKSTPTPKPDLNFPSNPIAPSTPKPAEANTSDEPSVPRAE
                     PAAPQEILTRLQIFLDQHSFGPGKIDGRWGEFTAKALVRYQIANGQKPTGQIDSDLQQ
                     QLAKIFPIYTTYTITDQDLKEVGNIPYKPAEQAKAKAMLYRSNAEFLGERYHSDPDFI
                     AKLNKDKNLDQLKTGDVIRVPNVPPFKIEDVREQPHIPINPEYAKRMIKIDTRYRMLD
                     VYDGDKLVCSFPITPGSKKLPAPIGTWKVVGIATLPWFRWDEAMLEHGYRSDNFYNIP
                     PGPRNPVGILWIGLNKKGIGIHGTNSPDTIGRSGSHGCIRLANWDAARILSQVTEGDQ
                     VLIF"
BASE COUNT          216 a          288 c          278 g          288 t
ORIGIN      
        1 gaacctttgc gtctttgcgt gagattttct tcttccctcg ttgcgtgatg ccttaaaaaa
       61 tcagcacctg atccccttcg gtgacctggc tcaggatccg ggctgcatcc cagtttgcta
      121 accggataca accgtgactc ccggaacgcc cgatcgtatc agggctattg gtgccgtgga
      181 taccgatgcc ttttttgttt aggccaatcc agagaatacc gaccggattt cgagggcctg
      241 gagggatatt ataaaagtta tcgctccggt acccgtgttc gagcatcgct tcatcccaac
      301 gaaaccacgg tagcgttgcg atccccacca ctttccacgt accgatcggg gccggcagct
      361 tcttcgatcc gggcgtaatc ggaaacgaac acaccagctt gtcgccatcg tacacatcta
      421 acatgcgata gcgggtatcg atcttgatca tccgttttgc gtactccgga ttgatcggga
      481 tatgcggctg ctctctaaca tcttcgatct tgaatggcgg cacattcgga actcgaatca
      541 cgtcgccggt cttcaattga tccaggttct tgtccttgtt gagcttggcg atgaaatcgg
      601 gatccgagtg ataccgttcg cctaagaact ctgcatttga ccggtagagc atcgccttcg
      661 ccttcgcctg ctcggccggt ttataaggaa tatttccgac ctctttcaga tcctgatccg
      721 ttatcgtgta ggtcgtgtaa atcgggaaga tttttgcgag ctgttgttga agatcagagt
      781 caatttggcc cgtcggtttt tgtccattcg caatctggta ccggaccagg gccttagccg
      841 tgaactcgcc ccaacgcccg tcgatcttgc caggaccaaa agaatgttga tccaagaaaa
      901 tttgtagccg ggtcaaaatc tcctgaggag cagctggctc ggctcgcggt acggagggtt
      961 cgtccgaggt attcgcctcc gcaggtttcg gagtcgatgg cgcaatcgga ttgctcggaa
     1021 aattcagatc cggtttcggg gtcggagtac ttttccctgt cggtttctcg
//
LOCUS       DATMKH010000185         1204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2320778, whole genome shotgun sequence.
ACCESSION   DATMKH010000185 DATMKH010000000
VERSION     DATMKH010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1204
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2320778"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="VNV63_02285"
     CDS             complement(<1..497)
                     /locus_tag="VNV63_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HXC61480.1"
                     /translation="MNLNNFTIKAQEAIAKAQQLAFDGSNPTIDTDHLLKALLTEEDS
                     LVEFLLKKNNVNVAFVESKLDESLQKLPKVSGGDPAQSINRDLNNAILHAGSDLKQFG
                     DEFVSVEHLLLGILQGKDNAAKLLRDAGLTEKGLITAIKDLRKGSTVNSQTQETTFNA
                     LNKYA"
     gene            576..>1204
                     /locus_tag="VNV63_02290"
     CDS             576..>1204
                     /locus_tag="VNV63_02290"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HXC61481.1"
                     /translation="MSYPRRFTCLGFSLFPTLLLFCLSTSAQRKPEVPVDHRFDALDQ
                     LFKQDQKDLGQFAALVWKDGKIVYQKQASDDFTPKTQAPMVRVADWMVAAVVMTFVDE
                     GKMTLDDKVTTYIPMFGKYMKGYITIRNCLTNTTGIKVSEDIKKALEKKKDDEDQNDE
                     TLEGVVNSYASHRDIGTNPGTEIFYSNLGPNIAARCLEVVSKKPFERLI"
BASE COUNT          277 a          347 c          282 g          298 t
ORIGIN      
        1 tttgcgtatt tgttcagcgc gttgaaggtc gtctcctgtg tctgggaatt gaccgtcgaa
       61 cccttccgga gatccttgat cgccgtaatc aatccctttt cggtcaatcc ggcatccctc
      121 aacagtttag ctgcgttgtc cttcccctgc aagatcccga gcagcagatg ctccacgctt
      181 acgaattcat ccccgaactg cttcaggtcc gaacccgcat gcaggatcgc gttgttcaag
      241 tcccggttga tcgattgcgc ggggtccccg ccgcttacct tcggcaactt ctgcaggctc
      301 tcatccagtt tggattcgac aaaagctaca ttgacattgt tctttttcag caggaactcc
      361 accagggaat cttcctccgt cagcaacgcc ttcaacaaat gatccgtatc tatcgtcgga
      421 ttggatccgt caaatgccag ttgctgcgcc ttggcgatcg cctcctgcgc ttttatcgta
      481 aaattgttta ggttcatata tctatggttt tccttttcga attatattgc agccttccgc
      541 cgcaaattcc aaaccagtac ctcaatccag ctattatgtc atatcctcgt cgttttacct
      601 gtctgggttt cagcctgttc ccaacccttc tgctattctg tctgtccacc tcggcccagc
      661 ggaaaccgga ggtgccggta gaccaccgtt ttgatgccct ggatcagctg ttcaaacagg
      721 accagaagga tctgggtcag ttcgccgccc tggtctggaa agacggcaag atcgtctacc
      781 aaaaacaggc ttccgatgat ttcacgccca agacgcaggc acccatggtc cgtgtcgcag
      841 actggatggt cgccgccgtg gtcatgacct tcgtagatga gggtaagatg acgcttgacg
      901 ataaagttac tacctatatc ccgatgttcg gaaaatacat gaaagggtat attacgatcc
      961 ggaactgcct aaccaatact acgggcatta aggtcagcga agacatcaaa aaagccctcg
     1021 agaagaagaa agacgacgaa gaccagaatg acgagaccct cgaaggcgtg gtgaactcct
     1081 acgcctccca ccgggacatc ggcaccaatc cgggcaccga gatcttctac agcaacctcg
     1141 gtcccaatat cgccgcccgc tgcctggagg tggtgagcaa gaagccgttt gaacggctga
     1201 tcaa
//
LOCUS       DATMKH010000186         1504 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1050040, whole genome shotgun sequence.
ACCESSION   DATMKH010000186 DATMKH010000000
VERSION     DATMKH010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1050040"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1497)
                     /locus_tag="VNV63_02295"
     CDS             complement(<1..1497)
                     /locus_tag="VNV63_02295"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HXC61482.1"
                     /translation="MEKLPDLAWFRERLTKLLNYPRYTVPTWKGHRYLYRRNDGLQNQ
                     SVIYTLKDLGDEPSVLLDPNQLSSDGTVAVTSTVLSDDGRFLAYGVAASGSDWNDIRV
                     RDIHTGEDLPDLVQWIKFSDPSWTKDSKGFFYSRFPEPQSSGNQTFAKLAYQKLCYHL
                     LGDPVQKDRVIYERQDDPGLGFAGEVSHDGRYLLMLVNKGTEERELLYFKDLGDPMAP
                     KIDAPFQPLVDKFEAEFKVVGLVGNRLYVVTDLNAARYKIVEIDLTNPGRDHWKEIVP
                     ESKDLLQDAALVGGKLVVKYLVDAKNQLFVFDLDGNRENEIGLPEPGTVGMLSGNPDR
                     PELFYSFTSFLYPMTIYRGDVSTGQNSVFQKPNVDFDPDRYETKQIFFASKDGTKVPM
                     FVVGKKGLKLDGNDPVYLTGYGGFNISMTPLFAASYASWLERGGVFALPNLRGGGEYG
                     RQWHEAGIKQHKQNVFDDFIAAAQALIDAKYTNARRIAIAGGSNGGLLV"
BASE COUNT          348 a          441 c          358 g          357 t
ORIGIN      
        1 caccagcaag ccaccgtttg agcctccggc gatcgcgatc cttctggcat tcgtgtattt
       61 cgcatcaatc agtgcctggg cggcagcgat gaaatcatca aacacatttt gcttgtgttg
      121 cttgatccca gcttcatgcc attgccggcc atactctcca ccgccacgca gattagggag
      181 agcaaaaact ccgcctcttt ccagccagct cgcgtacgac gccgcaaata aaggtgtcat
      241 cgagatgttg aatccgccgt aaccggtcag gtagactggg tcattaccgt ctaatttaag
      301 cccttttttg ccgacgacga acatgggcac tttggtaccg tcctttgagg caaagaagat
      361 ctgctttgtc tcgtatcgat ccgggtcaaa atccacgttc ggcttttgga acacgctatt
      421 ttggccagtg cttacgtccc cgcgatagat cgtcatcgga taaagaaatg acgtgaacga
      481 ataaaacagt tcgggacgat caggattccc ggacagcatc cctaccgttc caggctccgg
      541 caggccaatt tcgttttccc ggtttccatc cagatcgaag acgaaaagct gatttttggc
      601 gtccacgaga tacttgacga ctagcttgcc gccgaccagc gcggcatctt gcaacaggtc
      661 cttcgactct ggaacgattt ctttccaatg atccctgccg ggatttgtca gatcaatctc
      721 gacaatttta tacctcgcag cgttgaggtc cgtcacgaca tacagccggt tcccaaccaa
      781 ccccaccact ttgaactccg cttcgaactt atcgacgagc ggttgaaaag gagcgtcgat
      841 cttcggcgcc atcgggtcgc cgagatcctt gaagtataac aattcccgtt cctccgtccc
      901 cttattgacc agcatcagga ggtatcggcc gtcgtgcgag acttcgcccg caaaccccag
      961 accgggatca tcctgccgtt cgtagatcac gcgatccttc tgcactggat ctcccaaaag
     1021 gtgatagcag agcttctgat aagcgagctt ggcgaaagtc tgatttccag aagactgcgg
     1081 ttcaggaaac ctcgaataaa aaaatccttt actgtccttg gtccagctcg gatcagagaa
     1141 tttgatccat tggacaaggt ccggaagatc ctcgcccgtg tggatatcac gaacccggat
     1201 atcgttccag tccgatccgc tggcagcgac cccataagcc aggaagcggc cgtcatcgct
     1261 caagaccgta ctcgtgaccg cgactgtgcc gtcgctcgag agttgattgg gatccagcaa
     1321 cacgcttggc tcgtcgccca gatccttcag cgtataaatc acactctgat tctgcagccc
     1381 atcgttcctt cggtacaaat agcgatgacc cttccaggtc ggcacggtgt atcgtggata
     1441 attcaaaagc ttcgtcaacc tctcccggaa ccaggcgagg tctggcagct tctccatata
     1501 agcg
//
LOCUS       DATMKH010000187         2452 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2320981, whole genome shotgun sequence.
ACCESSION   DATMKH010000187 DATMKH010000000
VERSION     DATMKH010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2320981"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2452)
                     /locus_tag="VNV63_02300"
     CDS             complement(<1..>2452)
                     /locus_tag="VNV63_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61483.1"
                     /translation="QLFPANSSASDSSSGTPETRKKRELHLAIRAVEISNGHLFVSRP
                     DGEPHLELSHLSSLIEPDLLMQTFRISLKTESIQITDPRWSGSVDHLEAALLLRHGDL
                     EVKRLSISSKLFSTTLHGTVKMDRPVSIAINFETVASVTLIKQLFDLKRDWTGEARFI
                     GAVTGSYPDLAIKGPVSLHRLVLDHQPIGDATALLSYQDRVLTITDLTGGLLEGKTQG
                     NISLRLAESPPSFDLSLEFEKLHAEPLTFVLFPQFNLSMQNTPESPAGAHHDVSPPEL
                     FKGKMLSGKLSLAGSGMAIAALSGDGWLTVAQDHPSDIPAADVATPLALLAAVQEGTI
                     RFHTAQGLWEINQADFKTPQSSLTIRGSIHPDQAIQFTAHLNTQRLNEIAASLGLPEL
                     DGQGTVTANLAGNLADPSMQGEIALKSLQIHNHPLGSLSSRFQYNHSLLALSQSEIRH
                     GESRTTGQGKLNLKSGQARSAFDLQIRFHHGDPQDLLALFKKEFPMTTPFSGEVKLKR
                     DSKALSAEGILTLTQGVILDQPIQAGEVSMKLSDDQLRITRISLKTRRSVMEGIGSFT
                     MDGHFSARLHSGNFHLEDFEWLRQKLPALQGHGPITAHGSGNLKNPEVSAQVTLQDMN
                     YAGLKLGEGRIELLVAQHEAKVMVSSEAGLKLQGTLQVGSPFRFNTELAVTKMRLDPW
                     IALWYPASVTKIQMQSSGLFTVNGDLENLKDSEVQARLSNLSLDLAGYQVNNDEEIDV
                     GYAQGQLAIRSFKLKGEGTSLTILGTVRFEKEMDLIFIGEADLGLLQLITPQIPYGKG
                     KTYLAMRLYNQWKDPKLRG"
BASE COUNT          534 a          632 c          706 g          580 t
ORIGIN      
        1 ccccgaagtt tcggatcctt ccattggtta tagagccgca tcgcgagata ggtttttcct
       61 tttccgtacg ggatctgggg tgtgatcaat tggagaaggc cgagatcggc ttctccgata
      121 aatatgagat ccatctcttt ctcaaagcgt acggtcccca agatagtcag agaggtgcct
      181 tcgcctttga gtttgaaaga tcggatagcg agctgtccct gcgcatagcc aacatcaatc
      241 tcttcgtcat tgttcacctg gtaaccggcg aggtcgagcg agaggttgga taatcgggct
      301 tggacctccg agtctttaag attctccagg tctccgttca cggtgaaaag cccgctggac
      361 tgcatctgga ttttggtcac cgaggcagga taccagagcg cgatccaggg atccaaccgc
      421 atcttcgtca cggccagttc ggtattaaac cgaaatggag agccaacctg taacgtacct
      481 tgaagcttca agcctgcttc tgatgagacc atcacctttg cctcatgctg ggctacaagc
      541 agttcgatgc ggccttcgcc aagtttaagc ccggcatagt tcatatcctg cagcgtcacc
      601 tgtgctgaga cctcggggtt ctttaggttt ccggatccat gggccgtgat cggaccgtgg
      661 ccttgaagag caggcagttt ttgacgaagc cattcaaagt cttccaggtg aaaattgccg
      721 gaatgaaggc gtgcggaaaa atgaccgtcc atcgtaaatg atccgatccc ttccatcacg
      781 ctgcgtctcg tcttgagcga gatccgtgtg attcggagct gatcatccga gagcttcatg
      841 ctcacctcac ccgcctgtat cggttgatcc aggatcaccc cctgtgtcag ggtcaggatg
      901 ccttccgccg ataacgcttt ggaatctctt ttcagcttga cctctcccga gaacggtgtg
      961 gtcatcggga actctttttt aaagagggcc aggaggtctt ggggatcacc gtgatgaaac
     1021 cggatctgaa gatcgaacgc cgaccgggcc tggccggact tcaggttcaa tttgccctgt
     1081 cctgttgttc ggctttctcc gtgccggatc tcgctctggg aaagcgcgag cagcgaatgg
     1141 ttgtactgga atcggctgga gagcgagccc aagggatggt tatggatctg aagacttttg
     1201 agcgcaatct cgccttgcat ggaaggatcg gcgagattgc ccgccaaatt ggcggtcacg
     1261 gttccttgcc cgtccagctc cggaagaccc agggacgctg cgatctcgtt gagccgttgt
     1321 gtattgagat gggccgtaaa ctggatggct tgatccgggt ggatgctccc gcgaatagtc
     1381 agggaggatt ggggagtttt aaaatcggct tggttgattt cccacagacc ctgggcggtg
     1441 tgaaaacgaa tagtgccctc ttggaccgca gcaagaagag ctaagggggt tgccacatca
     1501 gcggcgggaa tgtccgatgg atgatcctga gctacggtca accaaccgtc tccgctcaag
     1561 gcggcgattg ccattccgga tcctgcaagg gaaagttttc cggacagcat cttgccctta
     1621 aagagctcag gcggggacac atcgtgatgc gcccccgcag gggattccgg cgtgttttgc
     1681 atgcttaaat taaattgcgg gaacagaaca aacgtcagcg gttcggcgtg gagcttctcg
     1741 aattccaatg acagatcaaa gctgggagga gattcggcaa gtcttaacga gatgtttccc
     1801 tgtgtcttgc cctccagcag gccgcccgta agatccgtga ttgtgaggac ccggtcctga
     1861 taagagagca aggcggttgc atcgccaatc ggttgatgat ccagtaccag ccggtgaagc
     1921 gagaccggtc ccttgatcgc gaggtccggg taagatcctg tgaccgctcc gatgaaacgg
     1981 gcctcaccgg tccagtcgcg tttcagatca aaaagctgct ttatgagggt tacggaggcg
     2041 actgtctcaa agttgatcgc tatcgaaacg gggcgatcca tcttgaccgt tccatgaagc
     2101 gttgttgaga agagctttga tgagatggaa agacgcttga cctccagatc gccatgacgc
     2161 agcagaaggg ccgcttccag atgatccacc gatccgctcc accgcggatc ggtaatctgg
     2221 atagactctg tcttaaggct gattctgaaa gtttgcatca ggagatccgg ttcgatcagc
     2281 gaggaaagat ggctcaattc caggtgcggc tccccatcgg gccggcttac gaatagatgg
     2341 ccatttgaaa tctcgaccgc acggatggcc aggtgcagct ctctcttttt ccgggtttcc
     2401 ggggtccccg aggatgaatc cgaggcggaa ctgtttgcag gaaaaagctg ct
//
LOCUS       DATMKH010000188         1086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_726757,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000188 DATMKH010000000
VERSION     DATMKH010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1086
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_726757"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VNV63_02305"
     CDS             <1..201
                     /locus_tag="VNV63_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018970443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HXC61484.1"
                     /translation="LLGCGMVDPNVLEKVNEGRGDRAYDPEVLSGYAFGMGLDRLAMA
                     LFGLPDIRLLIENDQRFLAQFS"
     gene            234..>1086
                     /locus_tag="VNV63_02310"
     CDS             234..>1086
                     /locus_tag="VNV63_02310"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF013734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HXC61485.1"
                     /translation="MPALDRSAEEIAEALTFTGVEVEGVQVHGAGIDKVVVAQIESFQ
                     QHPNADRLSVCQVLDGSKTPRQIVCGAKNFRAGDKVPLALPGAVLPDGTKIKASKLRG
                     VESEGMLCSERELGFSDEASGLMILDPSAPIGVALGDLFPPDTIFDLEITPDRPDLLS
                     YIGLARELSIIFQIPGIKVSDPSAAAAAKPISDASQVRIDALEACPFAVFHEVRDVTV
                     GPSPDWMRRRLEAAGLRSINNIVDVTNYVMLETGKPIHAYDLSKLALPVIVRFARAGE
                     KLLALDGK"
BASE COUNT          224 a          270 c          319 g          273 t
ORIGIN      
        1 cttctcgggt gcgggatggt agacccaaac gttcttgaga aagtaaacga gggaagggga
       61 gaccgggcct acgatccgga ggttttatcg gggtatgcat tcggcatggg cctggacagg
      121 ttggcgatgg ccctattcgg tttgcctgac atccgtttgc tgatcgaaaa cgatcaacga
      181 ttcctagccc aattctcgta atgaagctgt cgcttaattg gctccgagaa tatttgccgg
      241 cgttagatcg gtctgctgaa gagatcgcgg aggctttgac attcactggt gttgaggttg
      301 aaggcgtcca ggttcacggc gctggtattg acaaggtggt agttgctcag atcgaatctt
      361 ttcaacagca tcccaacgcg gaccggctga gtgtctgcca ggttttagac ggttccaaga
      421 cgccgaggca aattgtctgc ggagccaaga atttccgggc cggagataaa gttcctttgg
      481 ctcttcctgg tgcggtttta ccggatggga ccaaaatcaa ggcgagcaag cttcggggag
      541 ttgaatctga aggcatgctc tgcagtgaac gggagctcgg tttttccgat gaggctagcg
      601 gcctgatgat tcttgatccc agcgcgccca ttggtgtcgc cttaggcgat ctctttccac
      661 ctgacacgat tttcgatctc gagatcacgc cggaccggcc ggatctgctg agttacatcg
      721 gtttggcccg cgaactaagc atcatctttc agattccggg tattaaggtg tcagaccctt
      781 cggccgctgc tgctgccaaa ccgatttccg atgccagtca ggtccggatc gacgcactag
      841 aggcctgccc gtttgccgtt ttccatgaag ttcgagatgt gaccgtcggt ccaagtccgg
      901 attggatgcg tcgccggctg gaggctgccg ggctccggtc catcaacaac attgttgatg
      961 ttactaacta tgtgatgctc gagaccggga aaccgatcca cgcttacgat ttgagcaagc
     1021 ttgctttgcc ggtcatcgtt cgttttgcgc gagctggaga aaaactactc gctttggacg
     1081 gcaaaa
//
LOCUS       DATMKH010000189         1379 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4565042, whole genome shotgun sequence.
ACCESSION   DATMKH010000189 DATMKH010000000
VERSION     DATMKH010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1379
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4565042"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1379)
                     /locus_tag="VNV63_02315"
     CDS             complement(<1..>1379)
                     /locus_tag="VNV63_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HXC61486.1"
                     /translation="WDKQPKADSRVFDARKSETDEFYELRIRGLDEDRKSVARQSAAG
                     LLWTKQFYQYIVKDWLTGDQYAPVPPASRWSGRNHDWQHVYSRDVISMPDKWEYPWFA
                     AWDLAFHMLPFAHLDSAFAKQQLELFLREWYMHPNGQIPAYEFAFSDVNPPVHAWAAW
                     RVYKISAPRGSRDIKFLESAFHKLLLNFTWWVNRKDTEGNNIFSGGFLGLDNIGVFDR
                     SKPLPTGGYLQQADGTAWMGFYCLTMLSIALELAQTRPAYEDMASKFFEHFIAICKST
                     NTFGGTGLWDEEDGFYYDQLKIDGRSIPLKTRSLVGLLPLVAVEILEEEQLESFKGFR
                     RRMEWFLTYRQDLAKLISIDSKLGHNHHLLAVPTKERLCRMLAYLLDENEFLSPYGIR
                     SLSKIHAAHPYIFRADGREYRVDYVPGEGDTHLFGGNSNWRGPIWFPINYLIVEALER
                     YYYFYGNDL"
BASE COUNT          337 a          362 c          363 g          317 t
ORIGIN      
        1 tgaggtcatt cccataaaaa taataatatc gctccagagc ttccacgatc aggtaattaa
       61 tcggaaacca gatcggtcct ctccagtttg agtttccacc gaaaagatga gtgtcccctt
      121 cgccgggcac ataatcgact cggtattcac ggccatccgc acggaaaatg taaggatgtg
      181 cggcgtggat cttggaaagg gaacggatgc cgtagggaga gaggaactcg ttctcgtcca
      241 acagataggc tagcattcga cagaggcgct ccttggtcgg caccgccaaa aggtgatggt
      301 tatgccccaa tttgctgtcg atcgaaatca atttcgcgag gtcttgccgg taagtcagga
      361 accactccat ccggcgccgg aaccccttaa aggactcgag ttgttcctct tcgagaattt
      421 cgaccgcgac cagcggaagc aatcccacca gagatcgggt cttaaggggg attgatctcc
      481 cgtcgatttt aagttgatcg tagtaaaatc cgtcttcttc atcccagaga ccggtgcccc
      541 caaaggtgtt cgtcgatttg cagatggcga tgaaatgttc aaagaacttc gacgccatgt
      601 cctcgtaagc gggccgtgtt tgggcaagct ccaaggcaat cgacaacatg gtgagacagt
      661 aaaatcccat ccaggccgtt ccatcggctt gctgcagata accacccgtg ggcaggggtt
      721 tagaccggtc gaagactccg atattgtcta acccgagaaa tccacccgaa aagatgttat
      781 ttccctcggt atccttgcga ttgacccacc aggtgaaatt caacagcaat ttgtggaagg
      841 cgctttcaag gaatttgatg tcgcgagatc cgcgtggagc gctgattttg taaacgcgcc
      901 aggccgccca ggcgtggact ggcggattga cgtcggaaaa ggcaaattcg taggccggaa
      961 tctggccatt agggtgcata taccattcac gcagaaacag ctccaattgc tgcttggcga
     1021 atgccgagtc cagatgggca aacggcagca tgtgaaatgc taaatcccag gcggcaaacc
     1081 aagggtattc ccatttatca ggcatcgaaa tcacgtcccg gctgtagaca tgctgccagt
     1141 catgatttct tcccgaccag cgactggcag gcggcacggg cgcgtattga tctccggtga
     1201 gccagtcttt gacgatgtat tgatagaact gcttggtcca gagcaggccc gcggccgact
     1261 gtcgagcgac actttttcga tcctcatcga gtcctcggat acgcagctcg taaaactcat
     1321 cggtctcgct tttccgagcg tcaaagactc tggaatctgc cttcggttgt ttatcccat
//
LOCUS       DATMKH010000190         1628 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1051447, whole genome shotgun sequence.
ACCESSION   DATMKH010000190 DATMKH010000000
VERSION     DATMKH010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1051447"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..581)
                     /locus_tag="VNV63_02320"
     CDS             complement(63..581)
                     /locus_tag="VNV63_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61487.1"
                     /translation="MNLFQTVLQSIENPDHVGSQQDLHGLVNLAQLLPGVQGAEQQMQ
                     PILSILGSHLQDALNEQQQNNGQAAVQQTVSNLSVPGAGVQEIINLFGQERFNALMGE
                     ISKRTGLDSQVILQFLPMLIPVVMRLLATGTHQTNPQAQNPVLNNFLGSNQNGGALLT
                     EAFQLASVFLHR"
     gene            739..1512
                     /locus_tag="VNV63_02325"
     CDS             739..1512
                     /locus_tag="VNV63_02325"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HXC61488.1"
                     /translation="MVLDSIGHFLRRRIFVLVFASALSALQTARCQSVAPPDPAPLVA
                     APNLTTANPFESEVTALEAKLSQSPISSGPVVFYGSSSIRLWKSLQQDFSGYAVLNCG
                     FGGSRLTDCVRYANRLVRPRKPSAIVIYAGDNDLAGGAPPERVFQSFKQLFAIFRTYS
                     PSIPIAFVSVKPSPARMGFLENILKFNRLVDDFLQTQPETEYIDVCSDMLGQDRKPIQ
                     SLFVSDQIHLSPAGYQILRKEIGEFLSQDCPPSKAIRSQ"
BASE COUNT          342 a          451 c          423 g          412 t
ORIGIN      
        1 cgctcatgtt ctcgaagttc ttctatctcg cgagcatgac cagctacttc acctttttcc
       61 cactaacggt gcaggaacac ggaagccagc tgaaaagctt cggtcagcag ggcgcccccg
      121 ttttgattgg aaccgagaaa attattcagg accggattct gggcctgcgg attcgtctga
      181 tgcgttccgg tcgcgagtaa tctcatgacc acggggatga gcatcggcaa gaattggagg
      241 atcacttgtg aatccagtcc agtccgtttc gagatctccc ccatcaatgc gttgaaccgt
      301 tcctggccaa aaagattgat gatctcctga actccggcac cggggacgga cagatttgaa
      361 acagtttgtt ggaccgcagc ctggccattg ttttgctgct gttcgttgag cgcatcctgg
      421 agatgggaac caagaatgct gaggataggt tgcatctgct gttctgctcc ctggacaccc
      481 ggaagaagtt gagcgagatt gactaggccg tgcagatcct gttggctgcc gacgtggtct
      541 ggattctcaa tcgattgaag gacggtctgg aagaggttca tggtgggacc tgcctttgta
      601 ccgcggcggc cgattggtag caagcgctga aaaatcgaac cggattttcg ggcgccttgc
      661 agcctccagt gttgacaagt aaaatatttg cttaatcgtt cttgcccgat tccgaagtaa
      721 ctgagttacg ccggtgatat ggttttggat tcaattggac attttttaag acgacggatt
      781 ttcgtccttg tttttgcgag cgcactttct gctctgcaga ccgcccgctg ccagagtgtc
      841 gcgccgcccg atccggcccc tctggtggcc gcccccaacc tgaccacggc caatccgttc
      901 gagtcagagg tcaccgctct ggaggcgaaa ctgtcccagt cgcctatctc ttccggtccg
      961 gttgttttct atgggagttc ttccatccga ctttggaaat cgcttcaaca agatttctcc
     1021 gggtacgccg tgctcaattg tggattcggc ggatcacgct tgaccgactg cgtccggtac
     1081 gccaaccgcc tcgttcgccc tcgaaaacca tcggccatag tgatttatgc cggcgacaac
     1141 gatctcgccg gcggcgcgcc gccggagaga gtctttcaat ctttcaagca attatttgcc
     1201 attttccgga cctattcccc gtccataccg attgcgtttg tctccgtcaa accaagtccg
     1261 gctcgcatgg gattcctgga aaatatcctg aaatttaacc ggctagtgga tgactttctg
     1321 caaacacagc ccgagacaga atacatcgat gtctgctccg acatgcttgg tcaggaccgc
     1381 aaaccgatcc aatcgctgtt tgtgagcgat cagatccacc tgagtccggc cggctatcag
     1441 atcttgcgaa aagaaatcgg tgagttcttg tctcaggact gtcctccgtc aaaggccatc
     1501 cgatcgcagt agaacgccga tcttcttcgc gaacaaatca cccgctcttt cggggtcagg
     1561 agaccaccag caggggccga tgcaaatttc actgtattga tcgccctatt cggattacgg
     1621 tctttccg
//
LOCUS       DATMKH010000191         5127 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2653261, whole genome shotgun sequence.
ACCESSION   DATMKH010000191 DATMKH010000000
VERSION     DATMKH010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 5127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5127
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2653261"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1561
                     /locus_tag="VNV63_02330"
     CDS             <1..1561
                     /locus_tag="VNV63_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HXC61489.1"
                     /translation="ERRAQTIPILTPSEKDRLTEAFHPDHNTASYRNLRMGPNTGDKT
                     VHELADLLEGDSMLDEKNLSLKPDYTVDVLVVGGGGAGCAAALMAHQEGADVLLATKL
                     RLGDSNTVMAQGGIQVAIGEDDSPINHFVDTMKGGHHKNNPGLLKMMVENGPAIADWL
                     LSLGVMFDRDEAGNLKVRKGGGSSKPRLLTCSDYTGLEIIRVLKDEVLNRGIRVQEFA
                     PVVELLSDENGNCSGAVLKDLDNQRYLIVSAKTVILATGGIGRLHIQGFPTSNHYGAT
                     ADALPLAYRMGANLIYIDTFQYHPTGVIFPEQLAGALITEGLRSDGAQLVNRHGKRFV
                     NEMDTRDVVSSAIIRECKEGRGVMTSAGRDGVWIDIPIVDFVNGPGTIEKRFPAMLKQ
                     FERYHIDIRREPVLTYPTLHYQNGGVQIDVNAESTVKNLFVAGEASGGLHGLNRLMGN
                     SLLDIMVFGQKAGKATAARSQNMTREKITLNHLKKFRSELKKSGITPKALSPMVLPHY
                     VRREEIHPASV"
     gene            1558..2718
                     /gene="sucC"
                     /locus_tag="VNV63_02335"
     CDS             1558..2718
                     /gene="sucC"
                     /locus_tag="VNV63_02335"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012009945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HXC61490.1"
                     /translation="MNIHEFQGKKLFAAYQIPTPRGVVHSNLSGIQESLNQLSSAVYV
                     VKAQIHAGGRGKAGGVKLTRNKAEVSSLAQGLLGKRLVTHQTGPQGREVKKVLIEDGL
                     EIDRELYLSLLIDRQAAAPAFIASGQGGMEIEELAEKAPEKIIKVWVDPAAGYQLYHG
                     RNIGFQLGLDREMIGSFVTLLGNLYRLFIEKDASLIEINPLVVTKTKAIVALDAKMTF
                     DDNALFRHEDIRVLRDLDEEEPLETQAAKYNLNYVKLNGEIGCMVNGAGLAMATMDLI
                     KLAGSEPANFLDVGGGATKETVSQAFQILLADSNVRGVFVNIFGGIVRCERIAGGIIE
                     AAKEVKLTVPVVVRLEGTNAKEGREMLKSSGLKLEVAETMWQGAQKIVQMVR"
     gene            2742..3656
                     /gene="sucD"
                     /locus_tag="VNV63_02340"
     CDS             2742..3656
                     /gene="sucD"
                     /locus_tag="VNV63_02340"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009370151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HXC61491.1"
                     /translation="MGILVDKHTKVMVQGITGREGSFHADQCLAYGTKVVCGVTPGKG
                     GIRWKGESSGKEAPVFDTVEEAFDETRADTALIFVPPAAAADAIMEATDAGLSLIICI
                     TEGIPIVDMMRVKRFLAGKRTRLIGPNCPGIITPDECKIGIMPGFIHKKGTVGLISRS
                     GTLTYEAVWQLTERQLGQSTCIGIGGDPLNGTNFIDLLEMFEKDPKTEAIVLIGEIGG
                     EAEEQAAEYIKKNMKKPVIAFIAGITAPPGRRMGHAGAIITSGKGTASEKMEALKSAG
                     VSVVENPARIGEVVEKRLHSKSAPQKKT"
     gene            3858..4121
                     /gene="groES"
                     /locus_tag="VNV63_02345"
     CDS             3858..4121
                     /gene="groES"
                     /locus_tag="VNV63_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HXC61492.1"
                     /translation="MKFKPLKDRVFVSYSEELEKTAGGIYIPDAAKEKPQKGKVEAVG
                     SEVKNVKAGDSILFDKYSGSKITMDNVEYLILKEEDILGILES"
     gene            4159..>5127
                     /gene="groEL"
                     /locus_tag="VNV63_02350"
     CDS             4159..>5127
                     /gene="groEL"
                     /locus_tag="VNV63_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HXC61493.1"
                     /translation="MPKQIIFSEQARAAILRGVNQLTDAVKATLGPKGRNAVLDKKFG
                     APTITKDGVTVAKEIELKDPYENMGAQLLKEVASKTSDVAGDGTTTATVLAQAIFREG
                     SKNVSAGANAMELKRGIDKAVEVVLEELKKMSKPCQAKKEISQIGTISANNDKTIGDL
                     IAEAMEKVGKDGVITVEEAKSMTTSLDVVEGMQFDRGYISPYFVTDPERMETVLEDAF
                     ILINEKKISSMKDLLPVLEQIAKMGRPLLIIAEDVEGEALATLVVNKLRGTLNCAAVK
                     APGFGDRRKSMLEDIAILTGGQALSEDLGLKLENVKVSDLGRAKRIT"
BASE COUNT         1324 a         1150 c         1541 g         1112 t
ORIGIN      
        1 ggagagacgc gcccagacga tccccatttt aacgccttcc gagaaggaca gactcaccga
       61 agcctttcac ccggaccata ataccgcgag ttatcgaaac ctccgcatgg ggccgaatac
      121 aggcgataag accgtccacg agctggctga tctcctagag ggcgacagta tgttggacga
      181 gaaaaacctt tcccttaagc ctgactacac ggtggatgtc ttagtggtcg ggggtggggg
      241 cgcaggctgc gccgctgcct tgatggccca tcaagagggt gcggatgtct tgttggcgac
      301 caagctacgg ttgggagatt ccaataccgt tatggcacag ggagggatcc aggtggcaat
      361 cggtgaggat gattcgccga tcaatcattt tgtggatacg atgaagggcg gccatcacaa
      421 gaacaacccc gggttgttaa agatgatggt cgagaacggg cccgcgattg ccgactggct
      481 gctgagtctg ggtgtgatgt ttgaccggga tgaagccggg aatcttaagg tccgaaaggg
      541 aggaggaagc agcaagcctc gattgctgac ctgcagtgac tacaccggcc ttgaaatcat
      601 ccgggttctg aaggatgaag tgctgaaccg cgggatccgg gttcaggagt ttgcgcccgt
      661 ggtggagctg ctgagcgatg agaatggcaa ctgcagcggc gcggttctca aggacctcga
      721 caaccagcga tacttgatcg tcagcgccaa gacggtgatc ctagccaccg gaggaatcgg
      781 gcggcttcat atacagggat tcccgaccag caaccattac ggcgccacgg cggatgccct
      841 gcctcttgct tatcggatgg gggccaatct catctatatt gacacctttc agtatcatcc
      901 cacaggcgtg atcttccctg agcaattggc cggtgctctg atcaccgaag gccttcggtc
      961 ggatggggcc cagcttgtga accggcatgg aaagcgcttt gtgaatgaga tggatacacg
     1021 agatgtcgtt tcgtcggcca ttattcggga atgtaaggaa gggcgcggtg tcatgacgtc
     1081 tgccggtcgt gatggcgtct ggatcgatat tcccatcgtg gattttgtga atggaccggg
     1141 aacgattgag aagcgcttcc cggcgatgct caaacagttc gaacgatacc acattgacat
     1201 ccgccgcgag ccggtgctca cctatcccac gctccattat cagaacggcg gggttcagat
     1261 tgatgtcaat gcagagtcta cagtgaaaaa cctttttgtc gccggggaag cttccggcgg
     1321 ccttcacggc ctaaaccgtc tcatggggaa ttctctcctt gacatcatgg ttttcgggca
     1381 gaaggctggg aaagctacgg ctgcaaggtc ccaaaatatg acgcgcgaaa aaataaccct
     1441 gaatcacctt aaaaagtttc ggagcgaact taagaaatcc ggcattacgc ccaaggccct
     1501 ttcgcctatg gtcttgccgc attatgttcg acgggaagag atccatccgg ccagcgtatg
     1561 aacatccacg agttccaggg taaaaagctc tttgcggcct accagattcc gactccacgc
     1621 ggcgtggtgc attccaacct gtcgggcata caagagtctc ttaaccagct cagcagcgcg
     1681 gtgtatgtcg tcaaggccca gatccatgcg ggcggtcgcg gtaaggcggg cggagtaaaa
     1741 cttacccgga acaaggccga ggtttcatcg cttgcgcagg gacttcttgg caaacgtctc
     1801 gtcacacacc agaccggacc gcaaggtcgc gaagtcaaaa aagttctgat cgaagatggc
     1861 ctggagatcg atcgcgaact ttatttgagt cttctaattg accggcaggc cgctgcgccc
     1921 gcatttatcg ccagcgggca gggagggatg gaaatcgaag agctggcgga gaaggcgccc
     1981 gagaagatca taaaggtctg ggtggatccg gccgccggct atcaactcta ccatggacgg
     2041 aacatcggtt tccagcttgg tcttgaccgg gaaatgatcg gctcctttgt aactcttttg
     2101 ggaaatctct atcgtttatt cattgaaaaa gatgcctctt tgatcgagat caatccgttg
     2161 gttgtgacca agaccaaggc aattgtcgcg ctcgatgcca aaatgacctt tgatgacaat
     2221 gccctctttc gacatgaaga tatccgggtg ctgcgggatc tggatgagga ggagcctctg
     2281 gagacccagg ccgctaagta caatctcaat tatgtaaaac tgaatggaga gatcggctgt
     2341 atggtcaatg gggcaggtct tgcgatggcg actatggatt tgatcaaatt ggcgggttct
     2401 gagccggcga atttcctgga tgtcggcggt ggggcgacaa aagagacggt atctcaggcc
     2461 ttccagatcc tccttgcgga ttcgaatgtc agaggggtgt tcgtaaacat tttcggcggg
     2521 attgtccggt gtgaacggat tgcaggcggg attatcgagg cggcgaagga ggtcaaactc
     2581 accgtcccag tcgttgtgcg actcgaaggc acgaatgcta aagaagggcg ggagatgctt
     2641 aaaagctccg gcttgaaact agaggttgcc gaaacgatgt ggcagggtgc tcaaaagatt
     2701 gtgcagatgg tgaggtagat attttttgag gttcctagac gatgggcatc ttagtagata
     2761 agcataccaa agtgatggtg caagggatca ccggccggga aggctcgttt catgcggatc
     2821 aatgcctggc ttatggtacg aaagtagtgt gcggtgtgac tcccggaaag ggtggaatac
     2881 gctggaaagg cgagtcttcc ggcaaagagg ccccggtttt tgacacggtc gaagaggcct
     2941 ttgatgagac tcgggccgat actgcgctga tatttgtgcc gccggctgcg gctgctgatg
     3001 ccatcatgga ggctacagat gcgggccttt ccctgatcat ctgcatcaca gagggaatcc
     3061 cgatcgtgga tatgatgcgt gtgaagcggt tccttgcagg caagcgcacg cggttgatcg
     3121 gtcccaactg tcctggaatc atcacgccgg atgaatgcaa gatagggatc atgccgggct
     3181 tcatccacaa gaagggtacg gtgggcttga tttcaaggag cggaaccctc acctatgaag
     3241 cggtctggca gttaaccgag cggcaactgg gccagtctac ttgcatcggg atcggtggcg
     3301 atccattgaa cgggacaaat tttattgact tgttagagat gtttgagaaa gatccaaaga
     3361 ccgaggcgat cgtactgatc ggtgagattg gtggagaggc ggaagagcag gccgcggaat
     3421 acatcaaaaa aaatatgaag aagcctgtga ttgctttcat cgcagggatc accgcaccgc
     3481 cgggacggcg aatgggtcat gccggggcaa tcatcacaag cggcaagggt accgcctcag
     3541 aaaagatgga agctttgaag tccgccgggg tttcggtcgt tgaaaaccca gcccggatcg
     3601 gcgaggtagt tgaaaaaaga ctgcattcaa agtccgcccc gcagaaaaaa acttgacagc
     3661 attctttatt ttaagctata gtgattagcg ctcaatagat gtgagtgcta attcagggga
     3721 gttgagccgt agtttaacga tgttctattc cagcggcggc cctagaggga atcattcgaa
     3781 tccgcgatag atagaatgta tcgaaacaag gaggaataaa gatggcgact gggactaaga
     3841 tcgagaagag agcaacaata aagtttaagc ctttaaaaga tcgagtgttt gtcagctata
     3901 gcgaagaact cgaaaagaca gcaggcggta tttacatccc ggatgccgct aaggagaagc
     3961 cacagaaggg gaaggtggaa gcagtggggt ccgaggtaaa gaatgtcaaa gctggcgatt
     4021 ccatcttgtt tgataaatat tcaggcagca agattacgat ggacaatgtg gaatacctga
     4081 tcctgaaaga ggaagacatc ctgggtattc tagagagtta aacattttta taatgacaaa
     4141 gtaaagggga agatatcaat gccaaagcag attattttta gcgagcaggc ccgtgcagcg
     4201 atcctgaggg gggtaaacca gttaaccgat gcggtaaaag ccaccttagg tcccaagggt
     4261 cgtaatgcgg tgcttgacaa gaagtttggg gcgccgacga ttaccaagga tggtgtgacg
     4321 gttgctaagg agattgagct gaaagatcca tacgaaaata tgggtgcgca gttgttgaaa
     4381 gaggtggcaa gtaaaacgag tgacgttgcc ggggacggca caacgacggc aactgtttta
     4441 gcccaggcaa tttttaggga aggtagcaag aatgtctcag ccggggccaa tgcaatggag
     4501 ctaaaacgcg ggatagacaa ggccgtagag gtagtgttgg aagaattaaa gaaaatgagt
     4561 aagccgtgcc aggccaagaa agagatatcc cagatcggaa ccatctccgc caacaatgat
     4621 aaaacgatcg gcgatctcat tgcagaggcg atggaaaagg tcgggaagga cggagtgatc
     4681 acggtggaag aggccaagag catgaccact tctttagatg tagtcgaagg tatgcagttt
     4741 gatcgtggtt acatctcgcc ctatttcgtc acggatccgg aacgaatgga gactgtttta
     4801 gaagatgcct ttattctgat caatgagaaa aagatcagca gcatgaaaga tctcctgccg
     4861 gttttagagc agatcgccaa gatgggcagg ccgcttctga ttattgccga agatgttgaa
     4921 ggagaggcat tggcaacgtt ggtggtgaat aaactccgcg ggactttgaa ttgtgcagcg
     4981 gtgaaggccc caggttttgg agaccggcga aagagcatgt tggaggacat cgccatcctt
     5041 accggaggac aagcgctttc tgaagatctt ggcttgaagc tcgagaacgt caaggtcagt
     5101 gatctcggac gtgccaagcg gatcact
//
LOCUS       DATMKH010000192         1352 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3597537, whole genome shotgun sequence.
ACCESSION   DATMKH010000192 DATMKH010000000
VERSION     DATMKH010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3597537"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1352
                     /locus_tag="VNV63_02355"
     CDS             <1..>1352
                     /locus_tag="VNV63_02355"
                     /inference="COORDINATES: protein motif:HMM:NF018208.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61494.1"
                     /translation="SPAAPAPVLTGVIGPAIQFVRCRPPYEALIAAVLDRVYVVETLE
                     KAIAIWQARVPMAPSTATDHDMTFVTLEGEIIDPSGMVSLDQLTLMIHHLGGEIADLQ
                     KKQGQKNEEIDTIHHQILEAQTVLRMREMELVQVKAAQMNLFQEIERLQQSLQDRKME
                     REMIEQESKEALSRLTQAKGLLAEKLAHQSEEGRRLAQMEQEGLQHHGTTAPLMEELT
                     QLRLEAAKLKERGVHIETMFGHHQSLLTDHRRGNAEKEAALKQLRAKRDHLAQELKSI
                     QSELSQGSQLLQELKAQREQKSAAHADRMKEIGRLETALASTRQTADKLRQEIHALEM
                     SRTELRMSLDHLVERLDSSYHRRMENLIQELGEFTLNPEETQDRLQQLRSKLSEIGQV
                     NLMAIEEYRGLEERHKFLTAQEADLTLSIETLQKVITQINRTTKKLFMESYEKLRQVF
                     "
BASE COUNT          364 a          357 c          386 g          245 t
ORIGIN      
        1 attcgcctgc tgcgcccgct cctgttctga caggagtcat tgggcccgcc atccagtttg
       61 tccgatgtcg tcctccgtat gaagccctga tcgcagctgt cctggacagg gtttatgtgg
      121 tggaaacgct cgaaaaagca atcgcgatct ggcaggcccg ggtacccatg gcgccttcta
      181 cggcaacgga tcatgatatg acattcgtta ccttagaggg agaaatcatc gaccccagcg
      241 ggatggtgtc cctggaccag cttaccttga tgattcacca tctgggaggc gagattgcag
      301 accttcagaa aaagcaaggg cagaaaaacg aagagataga cacaatccat caccagatct
      361 tggaagccca gaccgttttg cgtatgcggg agatggaact ggttcaagtc aaggcggctc
      421 agatgaatct ctttcaagaa atcgagcgcc tccaacaaag tctccaggat cgaaagatgg
      481 aaagagagat gatcgagcag gagtctaaag aggccctctc gaggctgacg caggccaagg
      541 gcttgcttgc tgagaagctg gcacatcagt cagaggaagg ccggcgactg gcccagatgg
      601 agcaagaagg tctccaacat cacggcacga cggctccgct gatggaagag cttacccagc
      661 tccgcctcga ggcggcaaag ctcaaggaac gcggggttca catcgagacg atgtttggac
      721 accaccaatc gcttctcacc gatcatcgcc gtgggaatgc cgaaaaagag gcggcattaa
      781 aacagctgcg ggcaaagcgc gatcacctcg ctcaggaatt gaagtccatt cagtcagagc
      841 tcagccaagg aagtcagctc cttcaggaac tcaaggcgca gagagagcaa aaatccgcgg
      901 cacatgccga tcggatgaaa gagatcggac gcctggagac cgctctggct tcgacccggc
      961 agacggcgga caaactcaga caggagattc atgccctgga gatgagccgg accgaactcc
     1021 gtatgagcct ggatcattta gtggagcgac tggacagcag ttatcatcgg cggatggaaa
     1081 atctaatcca ggaactcggc gagttcacgc ttaaccctga ggagacacag gatcggctcc
     1141 agcaactccg atcgaagctt tcggaaatcg gccaggtgaa cctcatggcg attgaggaat
     1201 atcgggggct ggaggaacgc cataaattcc tgaccgccca agaggccgac ctgacactgt
     1261 ccatcgagac gctccagaag gtgattaccc agatcaaccg gacgaccaag aaactcttca
     1321 tggaaagtta tgagaagctg cggcaggttt tt
//
LOCUS       DATMKH010000193         1108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_411000,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000193 DATMKH010000000
VERSION     DATMKH010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411000"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..376
                     /locus_tag="VNV63_02360"
     CDS             179..376
                     /locus_tag="VNV63_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61495.1"
                     /translation="MKRQSIAEMTLAKQVKNIETEIANLQLEESHIQAKLETLTNMRA
                     GMAQEIDRLREARAKASEIRK"
     gene            373..552
                     /locus_tag="VNV63_02365"
     CDS             373..552
                     /locus_tag="VNV63_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61496.1"
                     /translation="MMEIGFNKETAEAVFVMVLAATQAEGKDAYKMSREELYPYIVSA
                     LEDLKLYKQILEQKP"
     gene            789..1031
                     /locus_tag="VNV63_02370"
     CDS             789..1031
                     /locus_tag="VNV63_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61497.1"
                     /translation="MTPLDTLMESLDKRYLGVMDNDPLLAAPEDIDVIIAYHRRNRAN
                     SEAGIKPKKEAGPPVDLVAMGLIKKTVAEPVRRRIV"
BASE COUNT          325 a          274 c          294 g          215 t
ORIGIN      
        1 ttcatgtgaa ggacgatggg agtatggtct atatctataa gaaggaggtg gagcttgagt
       61 gatctattga ctctcgaaga atattgcaaa agacaaggtt ggccttatcc gctaccatct
      121 cctctaacaa ttgaggggca gctgcttatt gatcgaataa tggcaggaga attcaatcgt
      181 gaaacgccaa tcaatcgccg agatgaccct cgccaagcag gtcaagaaca tcgagacgga
      241 aatcgccaat ctccaactgg aggagtccca tatccaagcg aaactcgaga ccctgactaa
      301 catgcgcgct ggaatggcgc aggagataga ccgcctgcgt gaagcgcgcg ctaaagcatc
      361 ggagatccgc aaatgatgga aataggcttc aacaaagaga cagccgaagc tgttttcgtc
      421 atggtcttag ccgcgaccca ggcggaaggc aaagatgcat acaagatgtc ccgggaagag
      481 ctgtatccat atatagtctc cgcgctcgaa gatctgaaac tctacaagca aatcttggag
      541 cagaagccat gaatctatac ttaatatcgc aaagtgtcaa caatgattac gacacatttg
      601 attcggctat tgtagcagct gaatcagatg aggatgcacg aaggatgcac cccggtggcg
      661 actatgatga gcctgggaga acttggaccg ccgatcaaag caaagttcaa gtagaattaa
      721 tcggaatagc taaggaggat acgaaagccg gagttgttct agctagtttc aacgcgggat
      781 gatcgcaaat gacccccctc gacaccctaa tggaatccct cgacaagcgc tacctaggag
      841 tgatggataa cgatcccttg ctcgccgcgc cagaggatat cgacgttatc atcgcttacc
      901 accgccgtaa ccgcgctaac tccgaagcgg ggatcaaacc caagaaggaa gcgggaccgc
      961 cggtggacct cgtcgcgatg ggtttaatca agaagaccgt cgcagaaccg gtgaggagga
     1021 ggattgtatg aagctagaga ccggccgcgc gacaacggcc acccacatca ccatggcaat
     1081 ctgcccctgc ggatgcaaga gcctcctg
//
LOCUS       DATMKH010000194         2388 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_730247,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000194 DATMKH010000000
VERSION     DATMKH010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_730247"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="VNV63_02375"
     CDS             <1..419
                     /locus_tag="VNV63_02375"
                     /inference="COORDINATES: protein motif:HMM:NF013362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HXC61498.1"
                     /translation="SRPLIHMSNARFDRILVDAPCSGLGVLRRHPEGKWQKQEAMIAA
                     HSKRQQKILENVAPLLKKGGVLVYSTCSTEAEENETVVDDFLEKYPGFMLENLRTILP
                     PSAADLVTGRGFLNTMPNPYRMDFFFAARLKLKKDL"
     gene            416..1087
                     /gene="rpe"
                     /locus_tag="VNV63_02380"
     CDS             416..1087
                     /gene="rpe"
                     /locus_tag="VNV63_02380"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HXC61499.1"
                     /translation="MMGLSKKIAPSILSADFARLGEEIAKVEAAGADMIHVDVMDGHF
                     VPNFTIGPPIVKAIRKVTKLPLDVHLMMTNPDEFIPEFIQAGSNYLTVHVETCPHLHR
                     TIQSIKEKGVKAGVTLNPATPLCSIEEILPDVDLLLIMSVNPGFGGQSFIPRALEKLK
                     RARKMIDDRGLKVELEVDGGVKPENMAALSKAGADIFVAGSAIFGNPDYKTTIQKMRK
                     AIGSS"
     gene            1096..1446
                     /gene="arsC"
                     /locus_tag="VNV63_02385"
     CDS             1096..1446
                     /gene="arsC"
                     /locus_tag="VNV63_02385"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033580.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HXC61500.1"
                     /translation="MQDVTIYHNPRCGTSRTTLELIRKKGIEPKVVEYLKTPPTVKEL
                     DEILKLLSMEPRELIRTKEPEYVKHKLDSRKLTRAELIAAMVKYPILIQRPIVLANGN
                     AVLGRPPENVENIL"
     gene            1553..>2388
                     /locus_tag="VNV63_02390"
     CDS             1553..>2388
                     /locus_tag="VNV63_02390"
                     /inference="COORDINATES: protein motif:HMM:NF013568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61501.1"
                     /translation="MQQSGLDEARLSMALGWLQAKEVVSLEREETTTMVSLTELGAKY
                     QRLMIPEIRLIKEIRINRQLTIQRLREASELDPIELSTSLGNLKKSGVMRIAPGGLLQ
                     LTHDDIPADLSQVQDILGRLSGDRPLELNKLEGAVLTEQYQRDWGKTKIFKFQEKKSR
                     RFGLTQFGQTITQQLPSIEPSAHEISQLGPAMLKDGSWRLQIGHLRKYNISLRPPRIS
                     VGRKHPYRQFLDFVKYKLVSMGFEEMLGPLVETEFWNMDALYMPQFHPARDIHDVYFV
                     KEP"
BASE COUNT          614 a          626 c          656 g          492 t
ORIGIN      
        1 tctccaggcc gctgatccac atgagcaacg cccgctttga tcgaatcctg gtggacgccc
       61 cttgctcggg tttgggagtg ctgcggcggc atccggaagg caaatggcag aagcaggagg
      121 cgatgatcgc cgcgcattct aagcgtcaac aaaagattct ggagaatgtc gcgccgttgt
      181 tgaaaaaggg aggggtttta gtgtacagta cctgctcaac agaggccgaa gaaaatgaaa
      241 cggtggtgga tgacttcttg gagaaatatc caggcttcat gctggaaaac ctccggacga
      301 tcctcccgcc gtccgcagcg gatcttgtga ccggccgcgg cttcttaaat acaatgccta
      361 atccgtaccg catggacttc ttttttgcgg ccagacttaa acttaaaaag gacctatgat
      421 gggattatca aaaaagattg ccccttcgat tttgtccgcc gactttgccc gtcttggaga
      481 agagatcgcc aaggtggaag cggccggcgc agacatgatc catgtggatg tgatggacgg
      541 gcattttgtt ccgaacttca cgatcggccc gccgatcgtg aaggcgattc ggaaggtgac
      601 caaacttccg ctggatgtcc atcttatgat gaccaatccg gatgagttca tccccgaatt
      661 cattcaggcc gggagcaact acctcacggt tcatgtcgag acctgcccgc atctccatcg
      721 gacgatccag tccatcaaag agaaaggggt gaaagcgggt gtgacgctga acccggccac
      781 gccgctctgc agcatcgagg agattctgcc cgacgtggat cttctcctga tcatgtcggt
      841 caatccggga ttcggcggtc agagctttat cccgcgggcc ctggaaaaac tcaagcgtgc
      901 ccgaaaaatg atcgatgacc gcgggctcaa ggtcgaactg gaagtggacg ggggcgtcaa
      961 gccggagaac atggccgcgc tatccaaggc gggcgcggac atcttcgtgg ccggttcagc
     1021 catctttgga aatcccgatt acaaaaccac gatccaaaag atgcgcaagg cgatcgggtc
     1081 ttcatagatt agactatgca ggacgtgacg atctatcata atcctcggtg cgggacatcc
     1141 cgcacgacgc tggagttgat ccggaagaag gggatcgaac cgaaggtagt cgaatatctc
     1201 aaaacccctc cgacggtaaa ggagctggat gagatactga aacttctttc gatggagccg
     1261 cgcgaattga taaggaccaa agaaccggaa tatgtaaagc ataaactcga cagccggaaa
     1321 ctcacacgcg cagaactgat cgccgcgatg gtcaagtatc cgatcctgat ccaacggccc
     1381 attgtactag ccaatggcaa cgccgtcctc ggtcgccccc ccgaaaacgt cgaaaatata
     1441 ctgtaaaatg tcccaagaca ttacgcaact cattgccggc ctccatccct tggagaaaaa
     1501 agtcctgata gcgctcagga caaccaccca accacagtgg gagtccgagc tgatgcagca
     1561 gagcggactc gatgaggcga ggctgtccat ggccctgggc tggcttcagg cgaaggaggt
     1621 cgtgtctctg gagcgggaag aaacaaccac catggtctcg ctcacggagc tcggtgcaaa
     1681 ataccaacgt cttatgatcc cggagattcg gctgattaaa gagatacgta taaaccgtca
     1741 gctcacgatt caaagacttc gcgaggccag cgaactggat cccatcgaat tgagtacctc
     1801 actcggaaat ttaaagaaaa gcggtgtgat gcgcattgca ccgggcggtc ttctccaatt
     1861 gacgcatgat gacattccgg cggacctcag ccaggttcag gacatactgg gccggctttc
     1921 gggagatcgt ccgctagagt taaacaagct cgagggagcg gtgttgaccg aacagtacca
     1981 gcgggactgg ggaaagacca aaatattcaa gttccaagaa aaaaaatcga gacgcttcgg
     2041 attgacacag tttggacaga cgatcaccca gcagcttcct tctatcgaac cttcggctca
     2101 cgagatctct cagcttggac cggccatgct caaggacggt tcctggagac tccagatcgg
     2161 tcatttaagg aagtacaata tcagtcttag accgcctcga atcagtgtgg ggcggaaaca
     2221 tccgtatcgg cagttcctgg attttgtgaa gtacaaactg gtctcgatgg gtttcgaaga
     2281 gatgttggga ccgctggtcg agaccgagtt ctggaacatg gatgcgctct acatgcccca
     2341 attccatccg gcccgggata tccatgacgt ctattttgtc aaagaacc
//
LOCUS       DATMKH010000195         1380 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3297464, whole genome shotgun sequence.
ACCESSION   DATMKH010000195 DATMKH010000000
VERSION     DATMKH010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3297464"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..363)
                     /locus_tag="VNV63_02395"
     CDS             complement(31..363)
                     /locus_tag="VNV63_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61502.1"
                     /translation="MTACNHSSSMAQELFPPFIQSKLDPELLRRLEELTSLGQPDQRL
                     SVLVRTASEINLDQENLLQSRGVIINSKLGEILSVTLPAKSIQAVAGMEFVLRIELAR
                     KLKKRGDP"
     gene            complement(428..1006)
                     /locus_tag="VNV63_02400"
     CDS             complement(428..1006)
                     /locus_tag="VNV63_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61503.1"
                     /translation="MARLNPKLMLAVLAGIWVVLAAYRILSHEEPKRIPLTYRQGQAV
                     TKVTSTRGEERRDLTLKFDLLNWKPPAPGAGLKNIFLPLYFEPPKPPAPPPTIPHAPP
                     PTLPPPPAPPTPEEIAAEQARKELSEFRYIGYLNRGGKEQAFLSKDKNLFIVKLGDVI
                     TGTYILKDANNSSAVIQETQTKVEMTVPLTGS"
     gene            complement(1096..>1380)
                     /locus_tag="VNV63_02405"
     CDS             complement(1096..>1380)
                     /locus_tag="VNV63_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018288626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HXC61504.1"
                     /translation="LSISLEQYAGMAGKKVRELRETALKQFPIFEVSIVHRIGELSIG
                     DNIVLIVVGAEHRAQAFEACRWCIDELKRTVPLWKKEKTPSGEIWVEDRP"
BASE COUNT          309 a          382 c          354 g          335 t
ORIGIN      
        1 taatcaaaga caagaccacg ctccgattca tcatggatct ccccttttct ttaacttcct
       61 cgccagctcg atgcgcaaaa cgaattccat ccctgctacg gcttgaatgg atttggccgg
      121 aagtgtgacg ctcagaatct ctcccaattt tgagttaatg atcacccctc ggctttgaag
      181 taaattctcc tgatccaaat taatctccga tgcggtccga acgagaacac tgagcctttg
      241 atccggttgt ccgagactcg tgagctcttc caggcgacgg aggagttccg gatcgagctt
      301 ggactgaatg aatggcggaa aaagttcctg ggccatcgac gatgagtggt tacaggcagt
      361 catggcaaag acaagtccga tcacgcctgt tgcagaaacg cttgacctaa aaaatcccat
      421 ccggcgatca gcttccagtg aggggaactg tcatctcgac tttggtttgg gtttcctgta
      481 tcaccgcaga actattgttt gcgtctttta atatatacgt ccccgtgatc acgtctccca
      541 atttcacgat aaagagattc ttatcttttg agagaaaggc ctgctctttg ccgccgcggt
      601 tcagatagcc aatgtaacga aattcgctta attccttgcg ggcctgctca gccgcgatct
      661 cctccggcgt cgggggagcc ggaggtggag gcagggtcgg aggaggtgca tgcgggatgg
      721 ttggaggagg cgcaggcggc ttcgggggtt caaaatataa tggtaaaaag atatttttga
      781 gtcccgcccc cggcgcgggc ggtttccaat tcaacagatc gaacttgagc gttagatccc
      841 ggcgctcctc ccctctggta gacgtcacct tagtcacggc ctggccttgc cgatacgtga
      901 gtggtatccg cttgggctct tcatgagaca ggattcgata agcagccaga accacccaga
      961 taccggccaa gacagctaac ataagtttcg gatttaagcg cgccattttt aatttcccgt
     1021 gggggcccat ttatatctcc cggagggacc tatttactct tcccggaggg gcctattagt
     1081 cttcccggag gggccctaag gccggtcctc cacccagatc tcaccgctcg gcgtcttctc
     1141 ttttttccag agcgggacgg tgcgtttcaa ttcgtcaatg caccagcgac aggcctcgaa
     1201 cgcctgggcc cggtgctccg ccccgacaac aatcaataca atattatccc cgatagaaag
     1261 ctcaccgatt cgatggacaa tcgaaacctc aaagatcggg aactgcttga gcgcggtctc
     1321 ccgaagctcg cgaacctttt tccccgccat cccggcgtac tgctcgaggc tgattgagag
//
LOCUS       DATMKH010000196         1283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2013605, whole genome shotgun sequence.
ACCESSION   DATMKH010000196 DATMKH010000000
VERSION     DATMKH010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2013605"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..1018)
                     /locus_tag="VNV63_02410"
     CDS             complement(164..1018)
                     /locus_tag="VNV63_02410"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HXC61505.1"
                     /translation="MRSTIVLFCFMILLLAVSNLKAQELANVNGTSISMADFQARLNE
                     LPPDVRQSMVTAEGKKDFLENHVIPGELLLGEAKREGLEKDKEIAKKVEDARRGVLIN
                     AAAQKIVAEKVTDEAAKKYYEQHSQEFKEVHASHILVATEEEAKNIKKQLDKGADFSE
                     LVKKHSTDPSAAQNAGDLGFFTRDQMVKPFTDKAFSMKVNQISDPVKTQFGYHIIKVI
                     EIRPAKKFGELDESRVVTVKRAIFNEEMDKLKAKAKITVHAELLKTDKSPEASGGEMN
                     GTKSKSDH"
     gene            complement(1120..1275)
                     /locus_tag="VNV63_02415"
     CDS             complement(1120..1275)
                     /locus_tag="VNV63_02415"
                     /inference="COORDINATES: protein motif:HMM:NF016056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HXC61506.1"
                     /translation="MEAAREILRYLPFGRPLAWLFYLPGVPFLADRLYIWIARNRYFW
                     FGHAKPH"
BASE COUNT          270 a          341 c          306 g          366 t
ORIGIN      
        1 cttggctccc atcccttcca gttctttgtt gacctcttta atccgattcg gccgttcctg
       61 tatcgtttta cgaccctcat ctgtcagggt cgtgagcatg atgtaggttg ccatttctcc
      121 tcctacggtg attactctaa tggtttttat agagcaggct gatttagtga tctgattttg
      181 atttcgtccc gttcatttcc ccgccagaag cttccggact cttatctgtc tttaagagct
      241 cggcgtggac cgtgatcttg gccttggcct tgagtttgtc catttcttcg ttaaagattg
      301 cccgcttcac ggtcacgacc ctggactcgt cgagctctcc aaacttcttg gccggcctga
      361 tctctatgac tttaatgatg tgataaccaa actgggtctt gaccggatcg ctgatctgat
      421 tgaccttcat cgaaaaggcc ttatctgtaa aaggcttcac catctggtcc ctggtaaaga
      481 accccaggtc gcccgcattc tgtgcggccg aggggtccgt tgagtgcttc ttgacgagct
      541 cactaaaatc ggcccccttg tcgagctgtt tcttaatatt cttggcctct tcttcggttg
      601 ccaccagtat gtggctggcg tggacctctt tgaattcctg gctgtgctgc tcataatatt
      661 tcttggccgc ctcgtccgtg accttctcag ccacgatctt ctgcgccgcg gcgttgatca
      721 agacgccccg cctggcgtct tcgacttttt ttgcgatctc tttatctttc tcgagccctt
      781 cgcgctttgc ctcacccaga aggagctctc cggggatgac gtggttttcc aaaaaatctt
      841 ttttgccttc agccgtgacc atgctttgtc ggacatcggg tggaagctcg ttcaaccttg
      901 cctgaaaatc ggccatcgag atcgaagtgc cgttcacgtt ggccaactct tgcgctttaa
      961 gattggacac agccaacagg aggatcataa agcagaataa tacgatcgta cttctcatat
     1021 aaaactcctt tcttaaaaaa aggtgaaggg tggcatcctt gcccccagag atggaaccat
     1081 caacctagca tattaaacgc aaaaaaagca agccaaattt cagtggggtt ttgcatgacc
     1141 gaaccagaaa tagcggtttc gggcgatcca gatatagaga cggtccgcaa gaaacgggac
     1201 gcctgggaga taaaagagcc aggccagagg tcggccgaaa gggaggtaac ggaggatttc
     1261 tctggcagct tccatgccat gga
//
LOCUS       DATMKH010000197         1080 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_412678,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000197 DATMKH010000000
VERSION     DATMKH010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412678"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1026)
                     /locus_tag="VNV63_02420"
     CDS             complement(<1..1026)
                     /locus_tag="VNV63_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61507.1"
                     /translation="MPDEAKKIGDLSDPGAKIVWRTWKADYELFQPEGTEPTEWASFD
                     GLTPWRELAFEGSGRTMILGSFFSFRDFNQAGNGRFGGPLVAQNRTYIRFEMRLNRVE
                     FDFIRDQQLYRQARLASAGGPRLRFPNSSVAVKAAWKIIKEEELPAAQGRYYLVDAMV
                     LDPLTHTCKMQKMGLVGLHIVQKTRTRPQWVWSSFEQIDNVPEPGAPLVPGRRFSLND
                     SSSPQVLDPAIPPPPISKSNPPLENPRPMQVIRSKKIADSTRKTNEDYQALLRGTVWE
                     NYQLVVTQWPKFPQPEEENGAPFPGQFTGPDPMTNIANTTMETYFQKSAATSCMTCHD
                     VARRKGTD"
BASE COUNT          236 a          295 c          291 g          258 t
ORIGIN      
        1 atcggttcct ttgcgccttg caacgtcgtg gcatgtcatg cacgaggttg ccgcgctctt
       61 ctgaaaataa gtttccattg tcgtattcgc gatgttcgtc atcggatcag gcccggtgaa
      121 ttgaccgggg aacggtgctc cattctcctc ttcaggttga ggaaactttg gccactgggt
      181 cacgaccaac tggtagtttt cccagacggt tcctcggagc agcgcctgat aatcttcgtt
      241 cgtttttctg gtggaatcgg caattttctt gcttcgtatc acctgcattg gtctcggatt
      301 ttccaacgga ggattgcttt tcgagatcgg cggaggaggg atcgccgggt ctagcacctg
      361 tgggctggac gaatcgttca gggagaaacg acggccggga acaagtggcg cgccgggttc
      421 cggaacattg tcaatctgct caaaggagga ccagacccat tgagggcgcg tccgggtttt
      481 ctggacaatg tggagcccga ccaaacccat tttttgcatt ttgcaggtgt gagtaagcgg
      541 atcaagcacc atcgcgtcga cgagatagta tcttccttgg gcggccggaa gttcctcttc
      601 tttgataatt ttccaggcgg ccttgaccgc aacggagcta ttggggaatc gaagtctagg
      661 accgccggcg gaggccaacc tcgcctggcg atacaactgc tgatcgcgaa tgaagtcaaa
      721 ttctacccta ttcaaccgca tctcaaaacg gatgtaggta cgattctgag cgaccaacgg
      781 acctccaaag cgaccgttgc cagcctgatt gaaatcccgg aaactaaaga aggaacccag
      841 aatcatcgtt cgcccggatc cttcaaatgc caactcgcgc caaggggtca gaccatcaaa
      901 agaggcccat tccgtaggtt ccgttccttc tggttgaaac agctcgtaat ccgccttcca
      961 ggtcctccaa acgatcttgg caccaggatc agaaaggtcg ccgatttttt tggcctcatc
     1021 cggcaccccc cgtattcccg gctttgccgg ccaattaaga gcgatgaacg cgcgccagga
//
LOCUS       DATMKH010000198         1978 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4568450, whole genome shotgun sequence.
ACCESSION   DATMKH010000198 DATMKH010000000
VERSION     DATMKH010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4568450"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(356..1081)
                     /locus_tag="VNV63_02425"
     CDS             complement(356..1081)
                     /locus_tag="VNV63_02425"
                     /inference="COORDINATES: protein motif:HMM:TIGR02292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HXC61508.1"
                     /translation="MTIDFDHGCGLLSPEDQEKMELILTKRLDSIHGAPALHGMLTAS
                     VVGPKEVPLDWILQAVLSHPRSESIALDHLPEFRWLVEKIEELFLRISLVFQQDPEMF
                     RPLVYMPNLKEGDTTPDPRSWCFGFAEAMMYHRKAWEPLLSMEGGFLTVAPILMTADP
                     DGWGENDSWNPFKQMTPTKLCEGLEMAARAIHAFWPFYAKLRSPIRTSIVPDRNDPCP
                     CGSGRKYKRGCGSPLNEALHPRR"
     gene            complement(1647..1901)
                     /locus_tag="VNV63_02430"
     CDS             complement(1647..1901)
                     /locus_tag="VNV63_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006613018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXC61509.1"
                     /translation="MSLRSTEPNFTPKQGQYLAFIYAYTHVLGRPPAEADLQRHFGVS
                     PPSVHQMVLTLERARLIRRKPGVARSIEVLVAPELLPVLR"
BASE COUNT          449 a          525 c          538 g          466 t
ORIGIN      
        1 tggacggacg tcaaagggca ggtcggtgtc ggcgtgccga caaagagagg atgagaaagt
       61 gtgaaccctt atcctgttca ccactacggc gcgcgcaaga agctggcccg cccggactgc
      121 ggttcaggtg aaaccaggcc tggctcttcg gacattcgtt gatccacaag aacgcatttt
      181 ctcaggagca gcccacgggt aagcgcgtga cgattttaac agaaaacgcg cttctcgaac
      241 tccggtgttt tcagagaagt tcaggagtcg gaggtagaag tagggagtga tgatccttcg
      301 agcactgaca ctttgatggg agttgccatc gaaccggaga cggcgtgatc caggttcacc
      361 ttcgagggtg gagagcctca ttcaacgggc ttccacaacc gcgcttatat ttcctgccgc
      421 tgccgcaggg acacgggtca ttccgatcgg gaacgattga cgttcggatc gggctgcgaa
      481 gtttcgcgta gaaaggccaa aaagcgtgga tggctcgagc cgccatttcc agtccctcgc
      541 atagctttgt tggagtcatc tgtttaaacg ggttccaact atcgttttca ccccatccgt
      601 ctggatcggc agtcatgagg attggagcaa ccgtcagaaa acctccttcc atcgaaagaa
      661 gcggctccca agctttccgg tggtacatca tggcttcagc gaaaccaaag caccagctcc
      721 gcggatcagg agtggtatcg ccctccttca gatttggcat ataaaccaga gggcggaaca
      781 tctccggatc ttgttggaac accaggctga tgcggagaaa caactcctcg attttctcca
      841 ccagccagcg gaattctggc aaatggtcga gggcgatcga ctctgatcgg ggatgactca
      901 ataccgcttg cagtatccaa tctaatggca cctctttagg gcccaccaca gaggcggtga
      961 gcattccatg cagggccggc gccccatgga tggaatctaa gcgcttggtg aggatcaatt
     1021 ccattttctc ctgatcctcc ggagagagaa gtccacagcc gtgatcaaag tcgatagtca
     1081 tatgatttac aaaaactaca atacgaaata acgtaattta cgtttgtatt aatctttcat
     1141 cgttgtcgag gtctaaatca tttttcccgt caaaaatcga aggtcgaaag ggaaccgggc
     1201 tgacaacggc tccggtccgc aagggaaaag acatcaaagg aaagatctct tattgagcag
     1261 caaccgggcg ggactaaggc tttggcttcc ggtagcgttc gagcttggcc catgaaatct
     1321 cggcgacgtc ggccagaagc cgctcggagc gccgggatta cgttgatact tgtcgagtac
     1381 gttcacgtcc ggctgcactg ctctctcacc ctccatgaag atgacgccga cggcgtcgct
     1441 tgggagcctg gcaatgaagc tttccgcatc ctcgataaat gcttgcccgt gtcgtagcaa
     1501 tgctgatccg cttgattgat cgcttcgata caaacagtcg catgtcttga ttcccttccc
     1561 aagtcgtcct cgtcgtcgtc gtccttcgtt ctcggccttt tcccggtggt acgagcgaaa
     1621 ctcctgcctc cttgcttcct tccctctcaa cgtaggaccg ggagcaactc gggagcaacc
     1681 agcacttcga tgctgcgagc aactccgggc tttcgtcgaa tcagtcgcgc tcgttcgagg
     1741 gtgagcacca tttgatgtac cgaaggcggg ctgacgccga agtgccgctg caaatcagct
     1801 tcggcgggag gccgtccgag cacgtgcgtg taagcataga tgaaggccaa gtactgtcct
     1861 tgtttgggcg taaagtttgg ctcggtggac cgtagtgaca tctggaattt ggattggtga
     1921 gtcttttttg ttctttaaac ccgtcaactt gtaatctccc attcctgctt gagtatat
//
LOCUS       DATMKH010000199         1109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2013762, whole genome shotgun sequence.
ACCESSION   DATMKH010000199 DATMKH010000000
VERSION     DATMKH010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2013762"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1109)
                     /locus_tag="VNV63_02435"
     CDS             complement(<1..>1109)
                     /locus_tag="VNV63_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HXC61510.1"
                     /translation="LGHWGTSPGQNLIYIHLNRLIKEHDANIIYIAGPGHGGPSLNAN
                     SYLEGTYTEVHPEITSDDNGMRLLFRKFSTPGGVPSHCGPHVPNSMHEGGELGYSLVH
                     AFGAAFDNPDLIVACVIGDGESETCPLEGSWKSVNFLNPVRDGAVLPILHLNGYKISG
                     PTVQGRTSDEDLKALYIGRGYKPYFVEGDKPEAVHQLLAQTLDDCYEEIQAIQQEARK
                     NGFKRQPVWPMIVLRTPKGWTCPKEVDGIPIEGTFRAHQVPLATVRENPEHLKILEAW
                     MRSYRPEELFDKNGKFIEELAELAPAGNRRMGGNPHVNGGRMLTTLALPDFTNYALEI
                     PGPGQVVAEAPRKLGDFFRDIYKLNKKNFRIFCPD"
BASE COUNT          233 a          307 c          299 g          270 t
ORIGIN      
        1 tcatcggggc agaatatgcg aaaattcttc ttgttcagtt tataaatgtc cctaaaaaag
       61 tctcctagct ttcgaggagc ttcagcaacc acttggccgg gtccaggaat ctccagcgcg
      121 taatttgtaa agtccggcag agcgagcgtg gtgagcatcc gtccgccatt cacgtgaggg
      181 ttgccaccca tgcgacgatt tccggcaggc gcaagctctg ctaactcctc aatgaacttg
      241 ccattcttat cgaacagctc ctccggccta tagcttcgca tccacgcttc gaggatcttg
      301 agatgttcgg ggttctcgcg cactgttgcg agcggtactt ggtgcgcgcg aaaggtgcct
      361 tctatcggga tgccatccac ctcttttggg caagtccatc ccttgggtgt ccgcaggacg
      421 atcatgggcc atacgggctg cctcttgaag ccgttctttc tcgcctcctg ctgaatggcc
      481 tgaatctctt catagcaatc gtccagcgtt tgggccagga gttgatgaac ggcttccggc
      541 ttatcgcctt cgacgaagta gggcttataa ccccgcccga tgtaaagggc cttcagatct
      601 tcgtcgctcg tccgtccctg aaccgtcggt ccagagatct tgtaaccatt gaggtgcagg
      661 atcggaagga ctgctccatc ccggaccgga ttcagaaagt tcactgactt ccagcttcct
      721 tccagtggac aggtctcgga ttctccatcg cctatcacgc acgcaacaat cagatcgggg
      781 ttatcaaacg cggcgccaaa ggcgtggact aatgagtaac ccagctcgcc gccttcgtgc
      841 atggaattag gcacgtgggg tccacaatgg ctgggtaccc cacccggcgt ggagaacttc
      901 cggaagagaa gccgcatgcc attatcatcg gaagtgatct ccgggtggac ttcggtatac
      961 gtgccttcca gatacgagtt tgcattgaga gaaggaccgc catgtccagg gccggcgata
     1021 tagatgatgt tcgcatcatg ctctttgata aggcggttga gatgaatgta aatgaggttc
     1081 tgtcccggtg atgttcccca atgcccgag
//
LOCUS       DATMKH010000200         1583 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1373462, whole genome shotgun sequence.
ACCESSION   DATMKH010000200 DATMKH010000000
VERSION     DATMKH010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1373462"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="VNV63_02440"
     CDS             <1..610
                     /locus_tag="VNV63_02440"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXC61511.1"
                     /translation="EKVAQTISQSGGRAIVYQADVRTKESVEQMVEAVLGQWDRIDLL
                     INNAGITRDDLLIKMDEACWDEVLDTNLKGSFHCIQAVAKGMIRQKSGHVVNIASLVG
                     LQGAIGQAAYSASKAGLIGLTIAAARELGPFGIQVNAVLPGLLPTEMGLNLSQDKREK
                     IIEANSLGRSTTMEDVADFVFHLSSIASVSGQVFNLDSRIAY"
     gene            620..1339
                     /gene="bioD"
                     /locus_tag="VNV63_02445"
     CDS             620..1339
                     /gene="bioD"
                     /locus_tag="VNV63_02445"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HXC61512.1"
                     /translation="MNSGLFITGTDTGVGKTTITAGLAVLLRSRHQPVGVMKPIQTGC
                     RRVNGKLFPNDTKILQWAAKTDDPLELVTPYRFHSPLAPFVAGRIERKRILPSKILRA
                     YRRLKQRHSFILVEGIGGILVPITKKDCVLELIQLLKLPLLIVARSGLGTLNHTLLTV
                     HQAREAGIPIVAIFLNTSRPQKQGLAEKTNPGILAELTGLPVFGPIPFIEGLQLNEGG
                     FKKILKVFQPWQDDLLKLTLP"
     gene            complement(1336..>1583)
                     /locus_tag="VNV63_02450"
     CDS             complement(1336..>1583)
                     /locus_tag="VNV63_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61513.1"
                     /translation="HGATVNLQRRYLKLGDEVIHTRYPQWGRGLVVEERNSQISGGIC
                     LVRVVFKDGVERSFLNDLDNYNCCYYAGVRISCDVTY"
BASE COUNT          392 a          396 c          404 g          391 t
ORIGIN      
        1 cgaaaaagtg gctcagacca tttcgcaatc gggaggccgg gccatcgtct atcaggcgga
       61 tgtgcggact aaagagtccg tcgagcaaat ggtggaggcc gtgcttggac aatgggaccg
      121 gattgatctg ttgatcaata acgccggcat cacaagagat gatctcttaa tcaagatgga
      181 cgaggcttgc tgggatgaag tcctggatac aaatctgaag gggtcatttc attgtatcca
      241 ggcggtggcc aaggggatga ttcggcaaaa gtcaggccat gtcgtcaata ttgcctctct
      301 ggtgggtctc cagggtgcga ttggtcaagc cgcttattca gcatccaagg cgggattaat
      361 cggacttacg atagcggctg cgcgggagtt aggaccgttt ggcatacagg tcaacgcggt
      421 cctgccgggt cttttgccga ccgagatggg tttaaatctc tctcaggaca aaagggaaaa
      481 aattatcgaa gcaaactctt tgggacgatc cacaaccatg gaagatgttg ccgattttgt
      541 ttttcatctg tcttctattg catctgtctc gggccaggtg ttcaatctgg acagccgcat
      601 tgcttactaa ctacgcagta tgaacagcgg attatttatc acaggcaccg ataccggggt
      661 cgggaagaca acgatcacgg ctggtttggc ggtactccta cgctctcgcc accagccggt
      721 cggtgtgatg aaaccgatcc agaccggctg tcggcgggta aacggaaaac tctttcccaa
      781 tgataccaaa atccttcagt gggcggcaaa gacagatgat cctcttgagc ttgtcacgcc
      841 ctatcgtttc cattctcccc ttgccccatt cgttgctgga cggatcgaaa gaaagagaat
      901 tttgccctct aaaattcttc gagcttatcg ccggcttaag cagcgccatt ctttcattct
      961 cgttgagggg atcggtggaa ttctcgttcc catcacgaag aaagactgtg tcctcgaact
     1021 gattcaacta ttgaagctcc cattgctgat tgttgcacgt tccggtctag gcaccttgaa
     1081 ccataccttg ttgactgtac accaggcaag agaagcggga atccccatcg tggccatctt
     1141 tctcaacact tcacggcctc aaaaacaggg cctggcagaa aaaacaaatc ctggaatatt
     1201 ggcagagttg acgggtttac ccgttttcgg tccgataccg tttatcgagg gattgcagtt
     1261 gaacgaaggt ggttttaaaa aaatcttgaa ggtttttcag ccttggcaag acgatctatt
     1321 aaaactgacc ctaccctaat aggtgacgtc gcaggagatt cgaacccccg catagtagca
     1381 gcagttgtaa ttatccagat cattgaggaa ggaccgctcg acgccgtctt tgaaaacgac
     1441 cctcacaaga cagatccctc ccgagatctg agagttccgc tcttctacta ccaaaccccg
     1501 gccccactga gggtatctcg tatgaatcac ctcatcgccg agcttcaggt atcgtcgttg
     1561 aaggttcaca gtagcaccat gtg
//
LOCUS       DATMKH010000201         1094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1692723, whole genome shotgun sequence.
ACCESSION   DATMKH010000201 DATMKH010000000
VERSION     DATMKH010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1692723"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1082)
                     /locus_tag="VNV63_02455"
     CDS             complement(<1..1082)
                     /locus_tag="VNV63_02455"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein N-terminal
                     domain-containing protein"
                     /protein_id="HXC61514.1"
                     /translation="MITSPTPIKRVSVADPNIAEAVIISPYQVLLNGRAPGGVSLLMW
                     DETDQSQTFEVSVDIDILSLNEKIHEVFPTEAIQLETSKDVVMLSGKISSVLVAAEIL
                     EIVKGSTPKVISLMTYPPTAVKEVLLEVKFAEVDRAAASQLGANIIRQFGSNMPMSVS
                     TGQFSPPSFQNSTPSTTAANGTTTGATPPQYNINNLLNISIFRPDINMAMMIEALETN
                     SVLQILAEPNLLTESGKEASFLAGGQFPVPVVQGSVAGGVAPITIQFKEFGVRLNFTP
                     TVMPDGLIHLNVKPEVSSLDYTNSVTISGTTVPALSTRRAESDMELRDGQSFAIAGLI
                     DNQVTEQFNKIPGISAIPILGKLFESR"
BASE COUNT          244 a          286 c          286 g          278 t
ORIGIN      
        1 cggctttcaa agagctttcc aagaataggt atcgcgctga ttccgggaat cttgttgaat
       61 tgctcggtca cttgattatc gattagcccg gcaatcgcaa aactctgtcc atctctcaat
      121 tccatatccg attccgcgcg ccgtgtagag agcgctggaa ccgtggttcc cgagatcgta
      181 accgagttcg tataatccaa cgagctgact tccggtttca cgttgagatg aatcagtcca
      241 tcaggcatca ccgtcggagt gaagttcaaa cggactccga attccttgaa ctgaatagtg
      301 attggggcca ccccgcccgc gaccgatcct tgcacaactg gaacaggaaa ttggccgcct
      361 gccaaaaagc ttgcttcttt tcccgattcg gttagcaggt tgggctccgc cagaatttga
      421 aggacgctgt tggtttccag cgcctcgatc atcatggcca tattaatatc gggccggaag
      481 attgatatat tcaatagatt attaatattg tattgaggtg gagttgcgcc tgtggttgtc
      541 ccgttcgcag cggttgttga tggcgtactg ttttgaaagc tcggtgggga aaactgccct
      601 gtggaaactg acatcggcat attgctgccg aactgcctga taatgttagc ccccaactgg
      661 ctcgccgccg cccggtcgac ctccgcgaat ttcacttcga ggagaacctc tttaacagcc
      721 gtcggtggat acgtcatcag gcttatcacc ttcggcgtag agcccttgac gatctcgaga
      781 atctccgccg ccacgagcac cgaggagatc tttccggaaa gcatgacgac gtctttggag
      841 gtttccagct gaatcgcctc ggtggggaag acttcgtgga ttttctcgtt caaactgaga
      901 atatcgatgt ccacggacac ttcgaacgtt tgactttgat ccgtttcatc ccacatcaaa
      961 agcgatactc cccctggcgc tcttccgttc aagagaacct ggtaggggct tatgatcacg
     1021 gcttcggcaa tattgggatc ggcgaccgag acgcgtttga tcggagtggg ggaagtgatc
     1081 accagggacc gacc
//
LOCUS       DATMKH010000202         2528 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_94673,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000202 DATMKH010000000
VERSION     DATMKH010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94673"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="VNV63_02460"
     CDS             complement(<1..490)
                     /locus_tag="VNV63_02460"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HXC61515.1"
                     /translation="MIPLYQFYEEIKDCQKCGLARSRTHLVFGGGNPNTQILFVGEAP
                     GFHEDQQGLPFVGRAGKLLSELLAAIGLQREDVYIANVLKCRPPENRNPEPIEIESCM
                     PHLWKQIELINPKVICTLGNFATQTILGKKVGITKIRGQHFQVKNFFVFPMLHPAAAL
                     HQG"
     gene            625..1956
                     /locus_tag="VNV63_02465"
     CDS             625..1956
                     /locus_tag="VNV63_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HXC61516.1"
                     /translation="MSMLRDKAVEIVKTLRKASFRAYWVGGCVRDLVMGQEPQDYDIA
                     TDAQPEQIMKLFPKTVPVGASFGVVKVLVEDCAFEVATFRSDGRYVDGRHPVEVHFSG
                     EKEDALRRDFTINGMFFDPIGDQFIDYVGGKQDIEAKLIRCIGDPNRRFSEDKLRLIR
                     AVRFTARFGYRIEPSTHEALVALAPQILEVSAERIRDELAKILMGPHPDQGVRLLHQT
                     GLLKVILPEVAAMEGVQQPPEFHPEGDVFIHTLLLLDKIRSPSFELAFGALLHDVGKP
                     PTFTIRDRIRFDNHSEVGSRMAREIGHRLRFSNQQIEVVAELVRDHLRFKDVRNMRSS
                     TLKRFLRNPTFTEHLELHRLDCLASHGDLSNWEFCKQKLDELEPEEIRPKRLLTGDDL
                     IGLGYSPGPQFAKILMAAEDAQLEGRVKTKEEAAQFVKKEFPLTSSGSASR"
     gene            2170..2514
                     /locus_tag="VNV63_02470"
     CDS             2170..2514
                     /locus_tag="VNV63_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HXC61517.1"
                     /translation="MTPYQPPPQPVEIEKRQDGQVRILWADGHEGIYPPVYLRAKCHC
                     ASCVEEWTGRRVVQEAHLPKDIRPLTINAVGTYAIQIEWSDGHNTGIYAFDLLREICP
                     CKRCLPEGIPQP"
BASE COUNT          615 a          646 c          703 g          564 t
ORIGIN      
        1 cgccctgatg aagagcggca gccgggtgca gcattgggaa aacaaaaaag tttttcacct
       61 gaaaatgctg gccccggatc ttggtgattc cgaccttctt gcccagtatg gtctgggtcg
      121 caaagttgcc tagggtgcag atcactttag ggttgatcaa ttctatctgt ttccagagat
      181 gtggcatgca ggactcgatc tcgatgggtt ccggattgcg attctccggc ggacgacatt
      241 tcaagacatt cgcaatataa acatcctccc gttgcagccc gattgcggca aggagctcgc
      301 tcaaaagttt accggctctg ccgacaaagg gcaggccttg ctgatcttcg tgaaaacccg
      361 gcgcctctcc gacaaacagg atctgagtgt tgggatttcc tccgccgaag acgagatgcg
      421 tccgagagcg ggcaagcccg catttctggc agtctttgat ctcttcgtag aattggtaga
      481 gagggatcat cgtgcgatgc tttctatcac cgctggaatc ttactgcatt tgattttaaa
      541 aatcaactgg aattgtcatt gcaagatgac agggtagcgg caattcctgt agaatataga
      601 atataatgtt ggggaagatc aaaaatgagc atgctgcgtg acaaagcggt agagatcgtg
      661 aagaccctcc gaaaggcaag ttttcgggct tactgggtgg gcggttgtgt acgcgacctg
      721 gttatgggcc aagagcccca ggactatgat attgcgaccg acgcgcagcc ggagcagatc
      781 atgaaactct ttcccaagac cgtgccggtc ggtgcaagct tcggcgtagt gaaggtcctt
      841 gtggaggact gcgcctttga agtggcaact tttcgttctg acgggcgata cgtcgatggc
      901 cggcatccgg tcgaggtcca tttctcaggc gagaaagaag acgctctccg acgcgatttt
      961 accatcaatg ggatgttctt tgatccgatt ggggatcagt tcattgatta tgtcgggggc
     1021 aaacaggata tcgaggccaa actcattcgt tgtatcggag acccgaatcg gcgattctca
     1081 gaggacaagc tgcggctcat ccgggcggtg cggtttacgg cccgattcgg ctataggatt
     1141 gaaccctcaa ctcatgaggc tctggtcgcg ttggccccgc agattttgga agtcagcgca
     1201 gaacgcatac gggatgagct ggccaagatc ttgatgggcc cccatccaga ccagggagtc
     1261 agactcctcc atcagaccgg tctattgaaa gtgatcctgc cggaggtagc cgccatggaa
     1321 ggggtgcagc aaccgccgga gttccatccg gaaggagatg tcttcatcca tacgctgctt
     1381 ctcctcgaca aaatacggtc tcccagtttt gaacttgcct tcggggcgct gctccatgat
     1441 gtcggcaagc cgccgacctt tacgatcaga gacaggatcc gattcgacaa ccactctgaa
     1501 gtcggcagcc ggatggcccg ggaaatcggc caccggcttc gtttctcaaa tcaacagatt
     1561 gaagtcgttg cggagctggt cagggatcac ctaaggttca aggatgtccg gaacatgcgt
     1621 tccagcacgt tgaagcggtt tcttcgcaat ccaactttta cggagcatct tgaacttcac
     1681 cgcctggact gtcttgccag ccatggagat ctctcgaact gggagttctg taaacaaaag
     1741 ctcgatgagc tcgaaccgga agagatccgg cctaagcgcc tgttgacagg agatgatctg
     1801 attgggctcg gctattcccc gggtccgcag tttgccaaga tcctgatggc cgcggaggat
     1861 gcacaactcg agggaagggt gaaaacaaaa gaagaggcgg cccagtttgt caagaaagag
     1921 ttcccgctta catcatctgg atcagcatca cgctgaaaac agtcagcagt atggcgatta
     1981 gcgtggcaag aatcaaccgg atcggggatt taaaattcag tcgatgcggc aagatgatcg
     2041 ctccttgtga acaagatcat tttgcctgac agtaaaaaac aagtcaagtc ctcgccctct
     2101 ggcttccggc ctatataata tcacggcgat gcttgaagac gagagatgaa atagtgtaga
     2161 ttaactagca tgacacccta tcaaccgccg ccgcaacccg tggagatcga aaaaaggcag
     2221 gacggccagg tccgcattct gtgggctgat ggccatgaag gaatctaccc ccccgtctat
     2281 ctccgtgcaa aatgtcactg tgcctcctgt gtcgaggaat ggacagggag acgcgtcgtc
     2341 caagaggcgc acctgccgaa agacatccgg ccgcttacaa tcaacgcggt tggtacatac
     2401 gcaattcaga tcgaatggag cgacgggcac aacacaggga tttatgcctt cgacctcctg
     2461 cgcgagatct gcccctgcaa gcgctgcctg ccggaaggaa tccctcaacc atgaagtctg
     2521 agaccatc
//
LOCUS       DATMKH010000203         3435 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_95086,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000203 DATMKH010000000
VERSION     DATMKH010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3435
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95086"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..853
                     /locus_tag="VNV63_02475"
     CDS             83..853
                     /locus_tag="VNV63_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HXC61518.1"
                     /translation="MTTVYLVTGVLGALIGSFLNVCIYRIPRGESIIRPRSRCPHCQS
                     TIRFYDNFPILSFLILRGRCRQCRAPISWRYPLVEMINTGGYLFLLWKFGLGWQLLVY
                     ALLFSALVVITFIDLSHRIVPDRITLPGMVVGALAAATVLPAGLVNALIGIFLGGGLF
                     YLIAMVGSWWLGQEAMGGGDIKLIAMIGAFLGWKDVLLTIFIAALTGSIIGLFLMIAF
                     GRDRKDALPFGPFLALGAVIALCWGSEILLWYSQLGSS"
     gene            857..2665
                     /locus_tag="VNV63_02480"
     CDS             857..2665
                     /locus_tag="VNV63_02480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXC61519.1"
                     /translation="MVRSFFTLLVVLLLIFFGLLNLHYTTLLDADRVLLVNEVAKHLV
                     VLSEQVAEELRGHQQQVLADPYYLYAIGTKRGIDRMVLLDQSARILSDSAGLSKPGTV
                     SPALGIRPAELAKVWAGGSVISPIYTDESKRQVKTMYLPLTDSGSQIVAVLGVSLDVS
                     EGDKAEQGAVTALLIKLFVTVAALVISYYLIKLFLVQRGIVQASGLMPGRGQMEKTGF
                     VIDTFHDLIQQLKAKEQELERLRSSAETRAARIEHYNENILQSVASGVITFNQKYVIT
                     TFNQAAERILDFFREEAVGKSCEELFGKESEITQFLEASIDRQQPITRQECGLRKKNG
                     ERIWVGVSTTLLRDPQNQVIGTTLVFSDLTEIKRLQEQVELKKRLMVLGEMSAGLAHE
                     IRNFMGTIMGFSRLLSKKLEAGDPKQRMVEAILAELNAMDRLIDDLLRYARTTELNIF
                     PLELEPLVRNVALRALNQAREPKPKLTISIAPDLPRVKLDEIQIRQALTNLIQNAIEA
                     MPQGGELRVTVSVRSMAQASTIKEIEISISDTGAGIPKDRMDRIFLPFFTMKEKGTGL
                     GLAIVHKIVLSHNGRIEVDSQEGMGTTFRLYFSSAA"
     gene            2679..>3435
                     /locus_tag="VNV63_02485"
     CDS             2679..>3435
                     /locus_tag="VNV63_02485"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXC61520.1"
                     /translation="MPTILIVEDKESMAGMLTQALEAEGYSTLHAKNGGEGIQLFKDK
                     KVDLVLTDLKLPQKDGLEVLAAVKAHNPLIPVIMMTAYGTIETAVKAVKEGAYDFLPK
                     PFDPDHLLVLVAKALEKQRLVTENIVLKDELAKQHLKFPRVLGKSPKILEVMEKTKKV
                     AAGNTTVLLLGESGTGKELFARAIHELSPRKDGQFVAINCAAIPRDLLESELFGHERG
                     AFTGAVGRKLGKFELADRGTIFLDEIGDMDLALQ"
BASE COUNT          775 a          829 c          995 g          836 t
ORIGIN      
        1 agggggtcca ttccttgagc ttgcctcggt tctgtggtat agtttggcaa tctgaaaaac
       61 ttgatcccat gggaaaaaag agatgacgac cgtctatctc gtgacaggtg ttctgggtgc
      121 cctgattggt agcttcctga atgtctgtat ctatcgtata ccccgaggcg aatccattat
      181 ccgcccacgc tcccgctgcc cgcattgcca gtccacgatc cgattctatg acaacttccc
      241 gatcctgagc ttcttgatcc ttcgggggcg ctgccgacaa tgccgcgcac ccatctcatg
      301 gcgctatccg cttgtagaaa tgatcaacac gggcggatac ctgtttctgc tctggaaatt
      361 cggcttgggc tggcagctct tggtgtatgc cttgctgttt tcagccttgg tggtgataac
      421 ctttattgat ctttcgcatc ggattgtgcc cgaccggata acgctgcccg gaatggtggt
      481 cggggctctg gcggcggcca ccgttttgcc ggcaggcctt gtcaacgctt tgatagggat
      541 ctttctaggc ggcggcttgt tttatctcat tgcgatggtg ggttcatggt ggctaggcca
      601 ggaagcaatg ggcggtggag acattaagct gatagcgatg atcggcgcct ttctgggatg
      661 gaaagacgta ttgctcacga tctttattgc agccttgacc ggatccatca taggcttgtt
      721 cctgatgatt gcattcggga gagatcgcaa agatgcccta cctttcggcc ctttcctggc
      781 gctgggagcg gtcatagctc tctgttgggg atcggagatc ctgctctggt acagtcaact
      841 ggggagttct tagacgatgg tccgcagttt ttttaccctt ctggttgttc tcctgctgat
      901 cttctttgga ctcttgaacc ttcactacac cactctcttg gacgcggatc gggttttgtt
      961 agtgaatgag gttgcaaagc atttagtggt tttaagcgag caggttgcgg aggaactccg
     1021 aggacatcaa cagcaggttc tggccgatcc gtattatctc tatgccatcg ggacgaaacg
     1081 gggtatagat cggatggttc ttcttgacca gtccgctcgg atcttgagcg actctgccgg
     1141 tttgtccaaa cccgggacgg tctcgccggc cttagggatc aggcctgctg aacttgccaa
     1201 ggtctgggcc ggaggatctg tgatctcacc tatctatacg gatgaaagca aacgccaggt
     1261 gaagacgatg tatctccctt tgacggattc gggttcacag attgtagcgg ttctgggtgt
     1321 cagcctcgat gtctcggagg gtgacaaggc ggaacaggga gcggtgacag cccttttgat
     1381 caaactgttt gtgacggttg cggccctcgt gatttcttat tatctcatca agctgttcct
     1441 cgtccagagg gggatagtac aggcgtctgg attgatgccg ggccgtgggc agatggaaaa
     1501 gaccggtttt gtcattgata cctttcacga tctgatccag cagctcaaag ccaaggagca
     1561 ggaactagaa cgactccgct cttcggcaga aacacgtgcg gctcgtatcg agcactataa
     1621 tgaaaatatc ctgcaaagcg tggccagcgg tgtaatcacg tttaatcaga agtacgtgat
     1681 taccaccttt aaccaggcgg ccgagcgaat cctggatttt tttcgagagg aggcggtcgg
     1741 aaaatcttgt gaagagctgt tcgggaaaga aagcgagatc acccagttct tggaagcatc
     1801 catagaccga caacagccga ttactcgtca ggaatgcggt ctccggaaga aaaatggtga
     1861 gcggatctgg gtcggcgtca gtaccactct tcttcgcgac cctcagaatc aagtgatcgg
     1921 gacaaccttg gtgttttcgg atttgaccga gatcaaacgc ctacaggaac aggtggaact
     1981 caagaaacga ttgatggtct tgggagagat gtcggccggt ctggcccatg agatccgaaa
     2041 ttttatggga acgatcatgg gtttttcccg gttgctctcc aaaaagcttg aggcaggcga
     2101 tcccaaacag cggatggtgg aggcaatcct cgctgaactt aacgctatgg atcggctgat
     2161 tgatgatctc ttgcgctatg cccgcaccac ggagctcaat atttttccac tggaactgga
     2221 gccgctggtc cggaatgtcg ccttacgggc attaaatcag gcaagagaac cgaaacccaa
     2281 actcacgatt tcgattgccc ctgatctccc gcgtgtcaag ctggatgaga ttcagatccg
     2341 gcaggcgttg accaacttga tccagaacgc gatcgaggcg atgccccagg gcggagaact
     2401 ccgtgtgacg gtcagtgttc gctccatggc gcaggcctca acaataaaag aaatcgagat
     2461 ctcgatcagc gatacagggg cggggatccc gaaggatcgg atggaccgaa tctttctgcc
     2521 gttctttacg atgaaagaaa aaggcaccgg tctgggcctt gccatcgtgc ataagattgt
     2581 cttgtcccac aacgggcgga tcgaggtgga cagccaggag gggatgggaa ctaccttccg
     2641 gctctatttt tcatcagccg catgatatag agaaccatat gccaactatc ctgattgttg
     2701 aagataaaga aagcatggcg gggatgctga ctcaggcctt ggaggctgaa ggttattcga
     2761 cccttcatgc caagaatggt ggagagggga tacagctgtt taaggacaag aaggtagacc
     2821 tggtcttgac cgatctcaag cttccgcaga aggatggtct tgaggtgctc gcggcggtaa
     2881 aagcgcacaa cccactgatt cccgtcatta tgatgaccgc ctatgggaca atcgagaccg
     2941 cggtcaaggc agtaaaggaa ggtgcgtatg atttcttgcc caagccgttt gatccggatc
     3001 acctcttggt cctggtggcc aaggcgcttg agaagcaacg cctcgtgacc gagaatattg
     3061 tcctaaaaga cgagcttgct aagcagcact tgaaatttcc cagagtcctc gggaaaagtc
     3121 ccaagattct ggaggtgatg gaaaagacca agaaggtggc ggctggcaac accacggtgc
     3181 tcctgctggg agagagcgga accggcaagg agttgttcgc acgggccatt catgaattga
     3241 gcccgcgaaa ggatggacag tttgtggcga tcaattgtgc ggcaatcccc agagatttgt
     3301 tggaaagcga gttgttcggc catgagcgag gggcctttac cggtgcggtg gggcgtaagc
     3361 tcggcaagtt tgaactggcc gatcggggga ctatcttcct ggatgagatc ggagacatgg
     3421 atcttgcact ccagg
//
LOCUS       DATMKH010000204         1204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3603884, whole genome shotgun sequence.
ACCESSION   DATMKH010000204 DATMKH010000000
VERSION     DATMKH010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1204
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3603884"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..977)
                     /gene="asnB"
                     /locus_tag="VNV63_02490"
     CDS             complement(<1..977)
                     /gene="asnB"
                     /locus_tag="VNV63_02490"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HXC61521.1"
                     /translation="MCGICGKLIFDRESRVSPAVLQAMTDSIRHRGPDDEGFYFSGQI
                     GLGFRRLSIIDLGGGHQPLSNEDASVWIVFNGEIYNYQELREFLLSKGHQFRTNSDTE
                     VIVHLYEELGEGCLERLRGMFGFAIWDDRRKSLFVARDRVGIKPLYYYLSGSSFIFGS
                     EIKAILADPEVKPEVVPEMIDRVLSFNYLPGEDTLLKNIHKLAPGSCILIKDGDVKIR
                     QYWDLQVPQSTLSLAAAEEQLSAMLEESVRMHMISDVPVGFLLSGGVDSTAMLCMAKG
                     KTDHSLRSFTVGFSDPGVTDERPYARLAAKAYGAEHHEMTISSKDFENF"
BASE COUNT          292 a          333 c          315 g          264 t
ORIGIN      
        1 aagaagtttt caaaatcctt ggacgagatc gtcatctcgt gatgttcggc gccgtaggct
       61 ttggccgcca gcctggcgta aggcctctca tccgtgacgc cgggatcaga aaaaccgacg
      121 gtaaaactgc gcagggaatg gtcggtcttc cccttggcca tgcaaagcat ggcggtcgag
      181 tcgacgcccc cgctcagcaa aaatccaacc ggcacatcgc tgatcatatg catgcggacg
      241 gattcttcca gcatggccga caattgttct tctgcggcgg cgaggctcag cgtggattgc
      301 ggaacctgaa ggtcccaata ttgcctgatc ttgacgtcac catccttgat caggatgcag
      361 gatcccggag ccagcttatg gatatttttc aggagtgtgt cttcaccggg caaataatta
      421 aagctgagta cgcgatcgat catttccgga acgacttccg gctttacctc cggatcagcg
      481 agaatcgctt tgatttccga accaaagata aacgagctgc ccgacaagta atagtaaagg
      541 ggcttgatcc ccacgcgatc cctggcgaca aatagagatt tgcggcggtc atcccagatc
      601 gcaaaaccga acattccgcg cagacgttcc aggcagccct cgcccaattc ttcatagagg
      661 tggacgatga cttccgtgtc cgaatttgtt ctgaattgat gtcccttgga caacaggaat
      721 tcgcgcaatt cctggtagtt atagatttcg ccgttgaaga cgatccagac gctggcgtcc
      781 tcgttggaaa gaggctgatg gcctcctccc aggtcgatga tcgaaagacg gcggaagccg
      841 agaccaatct gtccggagaa atagaaccct tcatcgtccg gcccgcgatg ccggatggag
      901 tccgtcatcg cctgcagcac ggccggagaa acccgggatt ctctatcgaa gattaactta
      961 ccgcagattc cgcacacagg gacctcattt cctactgggg gaagacgaaa gaaaaattct
     1021 tttgtcctga aacccttatt ttattgtcga agaggatgaa aaaaagaacg agccgcttac
     1081 ctgcgctcat cgccagatcg tctcccatcc caaaagccgc ccgagctgct ggcgccggag
     1141 atagcggatc gtcctggggt catcgacctg ggggatccct ttccaatatc cgctgagaaa
     1201 tccg
//
LOCUS       DATMKH010000205         2193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1377459, whole genome shotgun sequence.
ACCESSION   DATMKH010000205 DATMKH010000000
VERSION     DATMKH010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1377459"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..958
                     /locus_tag="VNV63_02495"
     CDS             29..958
                     /locus_tag="VNV63_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007232245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HXC61522.1"
                     /translation="MIKIIVGRLFQLIPVLWIILTLTFFMSKEVPGGPFSAERQATPE
                     VIGRLNAYYGLDKPLWEQYLGYLAKFIRFDLGPSYTYQSESVNEVIARSFPISLEIGT
                     LAFCIASVLGITAGVIAAANKNKLWDYFPMSLSMLGICLPTFVLGPVLALLFGLVLKL
                     APVSGWYGLNYVPFHPMIMPAVTLGLFYSAYIARLTRGGMLDILNQDFIRTARAKGVR
                     DHEIILKHALRGGLLPVVAFLGPAFAGLLSGSFVVEKVFNVPGIGQEFIKSVFNRDYP
                     LVLGTVAFFGILIVVFNLASDILQTLMDPKTRK"
     gene            963..2063
                     /locus_tag="VNV63_02500"
     CDS             963..2063
                     /locus_tag="VNV63_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXC61523.1"
                     /translation="MNSATPVATVSIEPAPVPVDQLEQGTSLWKDAWYRLRKNHVALF
                     GLYAFCVIAVLCLVGPYFTGYTYEQTQISLKASAPMEPVFLRSEALKTEGPKKSFVAL
                     SNLEDEFLEKPSTVRKEIVEKIARGGTYTEGKVTYGPSNQRHLFGTDPLGRDLLTRVL
                     IGGRISLAVGFAATVVSVGIGVLWGAIAGFVGGKMDTVMMRFADILYALPFTVIVILL
                     MVMFGRNIILLFVAIGAVEWLTMARIVRGQINSLKNQEFIEAAISLGLPGHRILFRHL
                     IPNVLGPIIIYATLTFPEVMLLEAVLSFLGLGVQPPLSSWGVLIDEGSRTMETSIWLL
                     VCPAFFFSLTLFSLNFLGDGLRDALDPKASKD"
BASE COUNT          455 a          547 c          607 g          584 t
ORIGIN      
        1 atctaaaggg tgagcgcgat ctagccaaat gataaagatc atcgtcgggc ggttattcca
       61 gctgattccc gtgctctgga tcatcctgac gttgactttc ttcatgtcaa aagaggtgcc
      121 gggagggcct ttcagcgcgg agcggcaagc gactccggaa gtgattgggc gcctgaatgc
      181 ctattacgga ctggataagc ccctatggga gcaatatctc ggctacctgg ccaaatttat
      241 ccgattcgat ttaggccctt cctacaccta tcaaagcgag tcggtcaacg aagtgatcgc
      301 ccgttccttt cccatctcgt tggagatcgg gacattggcc ttttgcattg cttcggtgct
      361 tggtatcacc gccggagtga ttgcagccgc gaacaagaac aagctttggg actactttcc
      421 gatgtcgttg tcgatgcttg ggatctgcct cccgaccttc gtcttaggcc cggtacttgc
      481 cttgctcttc ggtctggttc tcaagctggc acctgtcagt ggttggtacg ggctgaacta
      541 tgtgccgttc catccgatga tcatgccggc ggtgacgctc gggctgtttt attccgctta
      601 catcgcccgt ctaactcgag gcgggatgct cgatatctta aatcaggatt ttattcgaac
      661 ggctcgcgcg aagggagttc gtgaccatga aatcattctg aaacatgcgc tccgcggtgg
      721 attgctcccg gtagtagcgt ttctcggccc agcatttgcc gggctcctca gtggttcctt
      781 cgtggtggag aaggtcttca acgtcccggg tattggccag gaatttatca agtcggtctt
      841 caatcgggac tatccgcttg ttttgggtac ggtcgccttt ttcggaattc tgatcgtggt
      901 tttcaatctg gcctcggaca tccttcagac gttgatggat cccaaaacac ggaaatagga
      961 agatgaactc tgccacgcct gtcgcgacag tatcgattga acccgcaccg gtcccagtcg
     1021 accaattgga acaggggacg tcgctttgga aagatgcgtg gtatcgcctt agaaagaatc
     1081 atgtcgccct cttcggcctc tatgcgtttt gtgtgattgc agtcctctgt ctagttggtc
     1141 cgtatttcac cgggtacacc tacgagcaga cgcaaatcag tttgaaagca agtgcgccga
     1201 tggagccggt ttttttgcgt tccgaagctc tcaaaacaga ggggccgaaa aaaagcttcg
     1261 tcgccctctc gaatctcgag gatgaattcc ttgagaaacc gagcacggtt cgcaaagaga
     1321 ttgttgaaaa aattgctcga gggggcacct acacagaggg caaggtgaca tacggcccct
     1381 ccaatcaacg tcacctgttc ggcaccgacc cgctcggtcg cgatttgctg acccgcgtct
     1441 tgatcggagg acggatttct ctggcggtcg gttttgccgc tacggtcgtc tcggttggca
     1501 tcggggtttt gtggggtgcg atagcgggtt ttgtcggtgg gaagatggat accgtgatga
     1561 tgcgcttcgc ggatattctg tacgctctgc catttacggt gatcgtcatt cttctgatgg
     1621 tgatgttcgg ccggaatatc atcctgcttt ttgtcgcgat cggagcggtg gaatggctaa
     1681 ccatggctcg catcgttcgt ggtcaaatta actcattgaa aaaccaggaa tttatcgaag
     1741 cggcgatttc cctgggactg cctggacatc ggattttgtt tcgccatctg ataccaaacg
     1801 tgctgggacc gatcattatc tacgccacgc tcacttttcc ggaagtgatg cttctggagg
     1861 cggttctgag ctttctcgga ctcggagtac aaccgccact tagttcctgg ggtgtcttaa
     1921 tcgatgaagg atcccgcacg atggagacat caatctggtt gctggtttgc ccggcatttt
     1981 tcttttcgtt gacgctcttc tccttgaatt ttcttggcga cggtctccgc gacgcactcg
     2041 atccaaaggc gtcgaaagat tgattgcaca ggagttcagg agttgcagga gacgaagccc
     2101 tttggctgga aggtaaagat gtacagctgg aatcgaaaac aaacggtgag aagtaaggag
     2161 catcaacagg cgggagaaaa ccattctgta act
//
LOCUS       DATMKH010000206         1022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4263690, whole genome shotgun sequence.
ACCESSION   DATMKH010000206 DATMKH010000000
VERSION     DATMKH010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4263690"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="VNV63_02505"
     CDS             <1..652
                     /locus_tag="VNV63_02505"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HXC61524.1"
                     /translation="HPDCLEVARLPLSWISSGVGYVQDPSTLVACDLLDPKPGEVVLD
                     ACAAPGGKAGYMAQKMDNQGSLVACDYDKVRLGRLEQNLVRLGVINCKIVLIDWLQHA
                     SPFKPETFDAILVDVPCTNTGVIRRRTDVRWRLTPQDFPRMQHLQGRIVKSTIQFLKS
                     GGRLVYSTCSIEPEENERVVDYIDREVPELRFAEARASLPVHDHLDGAFAALFFKA"
     gene            complement(778..>1022)
                     /locus_tag="VNV63_02510"
     CDS             complement(778..>1022)
                     /locus_tag="VNV63_02510"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HXC61525.1"
                     /translation="EEYERILVGNMMNGERFETYVNYAVRGQGTIALNGAVAHLGKPG
                     HLLTIMSFGLFDPAEAGTHRPKVIILGEKNEVLRQK"
BASE COUNT          228 a          288 c          265 g          241 t
ORIGIN      
        1 tcaccctgat tgcctggagg tcgcccgctt gcctctctcc tggatctcct ctggagtcgg
       61 ttacgtgcag gatcccagta ctcttgtggc ctgcgatcta ctggatccga agccggggga
      121 agtggttctg gatgcctgtg cggcacccgg cggcaaagcc ggatacatgg cgcaaaaaat
      181 ggacaatcaa gggagcctcg tggcctgtga ttatgataag gtccgactcg gccgactcga
      241 gcaaaacctg gtccgactcg gcgtcatcaa ttgtaaaatt gtcctgatcg attggcttca
      301 acacgcttcc cccttcaagc ctgaaacttt tgatgcaatt cttgtcgacg ttccttgcac
      361 caacaccgga gtgatccgga gacgcaccga cgtgagatgg cgtctgactc cacaagattt
      421 cccacggatg cagcatcttc aaggccggat cgtcaagtcg acgattcaat tcctgaagtc
      481 cggcggccgt ctcgtctaca gcacctgcag tatcgaacca gaagaaaacg aacgagttgt
      541 tgattacatc gatcgggagg tccctgagct tcgctttgcc gaagccagag cctcgttgcc
      601 ggtccacgac catctcgacg gggcatttgc agcacttttt tttaaggcct gacgaagata
      661 ggagtcagga gtcaggagca cccctacggg aatcgcgcga agatttgagc agaagcttca
      721 gagaagtgca gcaggcgacg aacaccgcga atcctgcttt tttgacccca atcccaatta
      781 tttttggcga aggacctcgt tcttctcgcc cagaataatg actttcggcc gatgagttcc
      841 ggcttctgcc ggatcgaaca acccgaaact catgatggtc aataaatgcc caggttttcc
      901 gagatgtgcg acggctccgt tgagggcgat cgtaccttgg ccgcggaccg cgtaatttac
      961 gtatgtctca aatctttcgc cgttcatcat gttcccgacc agaatgcgtt catactcttc
     1021 ca
//
LOCUS       DATMKH010000207         1883 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3304205, whole genome shotgun sequence.
ACCESSION   DATMKH010000207 DATMKH010000000
VERSION     DATMKH010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3304205"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..706
                     /locus_tag="VNV63_02515"
     CDS             62..706
                     /locus_tag="VNV63_02515"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HXC61526.1"
                     /translation="MNYRKGFKYFLAILLIFAFSSSSTAGKIYRWMDENGVIGFTDDL
                     SSLPEKYLKTATPYDEAGPGERPTILEGMKPSQPSEELETPADVDNNGHDEAWWKNRI
                     QELKTQRDVLLQEKERLQEEYNTRYATWLNPFAPGNKNIGEINKQINSGKPVDPKLFF
                     GLPVPQPEIGNNLEELKKQIVQADEELKDIEYDLSVGLPEEARKANAPPGWLRD"
     gene            complement(730..1062)
                     /locus_tag="VNV63_02520"
     CDS             complement(730..1062)
                     /locus_tag="VNV63_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61527.1"
                     /translation="MKIPWKPLIMVALHKKGGSSTLQEIYSDLQVGVAKTYSQEKFTR
                     HIRPALAYLRKRGLTENPKRGFWKLSPGGHLEVAHMQARVSRIHPQMSTVPLHLLIEA
                     LRPFEESI"
     gene            complement(1086..>1883)
                     /locus_tag="VNV63_02525"
     CDS             complement(1086..>1883)
                     /locus_tag="VNV63_02525"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HXC61528.1"
                     /translation="AQDLSQQLSNPDLLILDARSGMDYFIGHLPGAVNISWKNFSNPN
                     SVMRGLLDPDPKGLEQKLGALGVGNNKTIIVYADPFKCYGDDARILWTLLSAGHQNVR
                     VLDGGWPKWRAEKQPIERGGVSPKPADFKIKPNPSIGITKEEVLERVQSKNADTVIID
                     SRTPEEYRGETGPGIPRGGHIPGAVNIPWNSFFKDDGMLKSPDEMRKVFEAQGVRPEK
                     EVITYCTAGVRAAHLFFTLRIAGYPNVKNYPGSWLEWSNDSSLPVER"
BASE COUNT          457 a          487 c          491 g          448 t
ORIGIN      
        1 ccgcaaggcc tccggtctcg atcccgccga ggcgctcagg tatgaataaa aggaggacac
       61 aatgaattat agaaagggtt ttaaatactt cctcgcgatc ttactgatat ttgccttttc
      121 aagttcatcc acagccggta aaatttatcg gtggatggat gagaacggcg ttatcggatt
      181 tacggatgat ctctccagtc tccctgaaaa atatctgaag accgcaaccc cgtatgacga
      241 agccgggccc ggcgaacgac cgaccatact tgagggaatg aaaccgtctc aacccagtga
      301 agaattagaa accccggccg atgtagataa taacgggcat gatgaggcat ggtggaaaaa
      361 tcgaatccaa gaactaaaga cccagagaga tgttctgctt caagaaaaag agcgtttaca
      421 ggaagaatat aatacgagat atgctacctg gctcaatcca ttcgctccag ggaacaaaaa
      481 tatcggcgag attaacaagc aaatcaactc tggaaaaccg gtcgacccaa aactcttctt
      541 tggattaccg gtccctcagc cggaaatcgg caacaattta gaggagctca aaaagcaaat
      601 cgtacaggca gatgaagaac taaaggatat cgagtatgac ctgagcgtgg gccttccgga
      661 agaagcgcga aaggccaatg cccctccagg ctggctgagg gattagcgcc ggactccact
      721 acttattatc tatatggact cttcaaaagg tctaagggct tcgatcaata agtgaagcgg
      781 tacggtcgac atctgtggat gtatccgtga cacacgcgcc tgcatatgag ccacttctaa
      841 gtgcccgccc ggtgatagtt tccagaaacc ccgtttcgga ttttcggtta gtcctctctt
      901 tctgagatac gcaagcgcgg gcctgatatg gcgcgtgaat ttctcctgac tgtatgtctt
      961 ggcaactcca acctgcaaat ccgaatagat ttcctggaga gttgaagatc cccctttttt
     1021 atggagggca accatgatta gcggtttcca tggaatcttc atgatcatct tttcccagaa
     1081 cttctttacc gctcaaccgg aagcgaagaa tcgttgctcc attcaagcca ggagccggga
     1141 tagttcttta cattcggata gcccgctatt ctgagtgtga agaacaggtg ggcggcccgg
     1201 acgccggccg tgcagtaggt gatcacctct ttctccggcc tgacgccctg ggcctcaaac
     1261 acttttctca tctcatccgg cgatttaagc atcccatcat ccttgaagaa cgagttccat
     1321 gggatgttca ccgcaccggg gatgtgtcct cctcggggga tgcccgggcc ggtctcgccc
     1381 cggtattcct cgggcgtccg cgagtcaatg attacggtgt cggcgttctt gctttgaact
     1441 ctttccaaga cctcttcttt tgtaatccct atggaagggt tgggcttgat tttaaagtcg
     1501 gccggttttg ggctgacgcc gccgcgttcg atcggctgtt tctcggcccg ccacttgggc
     1561 cagccgccgt ccagcacgcg gacgttctga tgccctgccg acagcagcgt ccagaggatt
     1621 cgggcgtcat caccgtagca tttgaacggg tcggcgtaca cgatgattgt tttgttgttg
     1681 ccgacaccca gcgcaccgag cttctgctcc agcccttttg ggtccggatc aagaaggccg
     1741 cgcatcaccg agttgggatt ggagaagttt ttccaagaga tgttcacggc accgggcagg
     1801 tggccgatga aatagtccat cccggatcgg gcgtcaagga tcaacagatc ggggttggaa
     1861 agttgttgtg acagatcctg tgc
//
LOCUS       DATMKH010000208         1422 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4266001, whole genome shotgun sequence.
ACCESSION   DATMKH010000208 DATMKH010000000
VERSION     DATMKH010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1422
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4266001"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="VNV63_02530"
     CDS             complement(<1..557)
                     /locus_tag="VNV63_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008506340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXC61529.1"
                     /translation="MVFKRYQERILIRVLFLFGTLSGASWLLVKGEYPYLVFLLLFIS
                     LQLVDFYNFHRKAQLEVEQFVESIHYRDFSRYFDVKEAPLELQSLRQGFNEINSTFKL
                     ISREKETQYQYLQKILELVNTGILSYEHKTGEIGWMNESFKNMLGVPYLKTIGSLERR
                     DANLYQEILNLRPGENKIVSLTKDK"
     gene            complement(563..>1422)
                     /locus_tag="VNV63_02535"
     CDS             complement(563..>1422)
                     /locus_tag="VNV63_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXC61530.1"
                     /translation="PTDANILILGENGTGKDLIAKAIHQYSLRAEKPYIKVDVGALTE
                     TLFESELFGHKKGAFTDAREDRMGRFEAGNGGTLFLDEIGNISLHQQAKLLSVLQNRV
                     VTRLGTNQPVPIDIRLICATNMPLTELANESRFRKDLIYRINTVEIMVPALRKREDDI
                     VLLARHFDKIYTNKYMKPLHDFDARAIEKLKSYHFPGNVRELQYTIERAVIMAEGDVL
                     QAKDLIFSPIESAPAEADEPQELKLSTIEKNAILKVIEKHNGNISKAAKELGLTRTAL
                     YRRLSKYDI"
BASE COUNT          324 a          438 c          295 g          365 t
ORIGIN      
        1 ttcttatctt tcgttagcga aacgatcttg ttctcccccg gccgcaggtt gaggatctcc
       61 tggtaaagat tggcatcccg tcgttccaaa gagccgatcg tcttcagata cggcaccccc
      121 agcatgttct tgaaggactc attcatccag ccgatctccc ccgtcttatg ctcataggac
      181 aaaatacccg tattcaccag ctccaggatt ttctgcaaat actgatattg cgtctctttc
      241 tcccgactga tcagcttaaa cgtagaattg atctcgttga acccctgccg cagggattgc
      301 agctcgagcg gcgcctcctt cacatcgaaa taacgggaga agtccctgta gtggatcgac
      361 tccacgaatt gctccacctc cagttgcgcc ttccgatgga aattgtagaa gtctaccagc
      421 tgcagactga taaagagcaa caaaaagacc aggtagggat actccccctt gaccagtaac
      481 catgacgccc ccgacagcgt gccgaaaaga aataaaaccc ggatcaatat ccgctcctgg
      541 taacgtttaa ataccatttt ctctatatat catatttact caatctccgg taaagcgccg
      601 tccgggttag cccaagctcc tttgccgcct tggaaatgtt tccgttgtgc ttttcgatta
      661 ccttaagaat cgcattcttc tcgatcgtac tcaacttgag ctcttgcggc tcatccgcct
      721 ccgccggcgc cgactcgatc ggcgagaaga tcaaatcctt cgcctgcaat acatccccct
      781 ccgccatgat caccgcccgc tcgatcgtat attgcagctc ccggacattc cccgggaaat
      841 gatacgactt cagtttctcg atcgcccgtg catcgaagtc atgcagcggc ttcatgtact
      901 tgttcgtata gatcttatca aaatgccgcg ccagcagcac gatatcatcc tcccgcttgc
      961 gaagcgccgg caccatgatc tccaccgtat tgatccggta gatcaggtcc ttccggaaac
     1021 ggctttcgtt cgccagctcc gttaacggca tattcgtcgc gcagatcagc cggatatcga
     1081 tcggtaccgg ttgattggtc cccagcctcg tcactacccg gttctgcaat accgatagca
     1141 gcttcgcctg ctgatgcaac gagatattcc cgatctcatc caggaacaaa gttcccccat
     1201 ttcccgcttc aaaacgtccc atccggtcct cccgcgcgtc cgtaaaggcc ccttttttgt
     1261 gaccaaagag ctcactttca aataaggtct cagtcaacgc ccccacatcc accttgatat
     1321 agggcttttc cgctcttagc gaatattgat gtatagcttt ggcaatcaaa tctttaccgg
     1381 ttccgttctc tcccaggatc aagatgttgg catccgtagg cg
//
LOCUS       DATMKH010000209         1194 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3607059, whole genome shotgun sequence.
ACCESSION   DATMKH010000209 DATMKH010000000
VERSION     DATMKH010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3607059"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /locus_tag="VNV63_02540"
     CDS             <1..737
                     /locus_tag="VNV63_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61531.1"
                     /translation="PLAPSSPRPLTDMSVSKNFNGAKWSPDGQRLVYSKGFNLYLAQS
                     DGTEPKRLITSKGIVSNPVWSPDGKSVRFTAAESVTSNVGTLFEVSAEGGEPHEVIPQ
                     WHRRSSQSFGKWTPDSKYFLFLAGTNGRTDIWAIRENRSFLNLRKREPVRLTAGPIRY
                     GSFAFSSDGKKIFTQGIQLRGQAERYDRKSAQFVPVRPALSAECCVYSNDGQWMAYVT
                     FPEGSLWRSKLDGSQRQQLTWPRWPL"
     gene            968..>1194
                     /locus_tag="VNV63_02545"
     CDS             968..>1194
                     /locus_tag="VNV63_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61532.1"
                     /translation="MKTHEIFTVPGSEGLWSPRWSPDGKSIVALTHATHSLMIGEIGA
                     GKWSELVGPSLEIFGYPQWSSDGSLVYYRNFS"
BASE COUNT          260 a          327 c          329 g          278 t
ORIGIN      
        1 tccctttagc cccgtcctct ccccgtccct tgactgacat gagtgtcagt aagaacttca
       61 acggggctaa gtggtcaccg gacggccaga ggctggtcta ctcgaagggc ttcaacctgt
      121 acctcgctca aagcgacggg actgaaccca agagacttat caccagcaaa ggtattgttt
      181 ctaaccccgt ctggtcgccg gacggaaagt cagttcgctt taccgcggcg gaatcggtaa
      241 ccagcaatgt cggcaccttg tttgaggttt cagcagaagg gggagagcca cacgaggtga
      301 ttccacagtg gcacagaagg tcttcccaga gcttcggaaa gtggactcct gatagcaaat
      361 attttctttt cctggcgggt acgaatggca gaacggatat ctgggccatc cgggagaatc
      421 gatcatttct gaatctcaga aaacgcgaac ccgtacgttt gacagcgggt ccgatacgct
      481 atggatcgtt tgccttcagc tccgatggga aaaagatttt cacacaggga attcagcttc
      541 gcggacaagc ggagcgatac gatcgcaagt ctgcgcagtt cgttcccgtt cgtcccgccc
      601 tctccgccga gtgttgtgtt tattcgaatg atggtcagtg gatggcctat gtgacttttc
      661 ccgaagggag tctctggcgc agcaagcttg atggcagcca gcgtcagcaa ctcacttggc
      721 cccgatggcc gttgtaaatc cgcattggtc tcccgacggg agggagatag ccttttcctc
      781 gctcttaccg gggaaaactt tgaaaacctt catcatcgca gcgcaaggcg gcacggtccg
      841 ggagctcacc caaagcgagt gtcctgaact tgatgcgaac tggtcccccg acgggacaca
      901 tctagttttc ggaagctttg ctatccggtc cggcgcatcg tgtccagagg tgctctacac
      961 tatagatttg aaaactcacg aaatcttcac ggtccccggc tccgaggggc tgtggtcgcc
     1021 gcgatggtcc ccggatggca agagcatcgt ggcccttacc catgctacgc attcactgat
     1081 gattggcgag atcggagctg ggaaatggtc cgaactcgtc gggccttctc ttgaaatctt
     1141 cgggtacccg caatggtcaa gtgatgggag cctcgtttat taccgtaact tctc
//
LOCUS       DATMKH010000210         1487 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2663868, whole genome shotgun sequence.
ACCESSION   DATMKH010000210 DATMKH010000000
VERSION     DATMKH010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1487
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2663868"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(549..1274)
                     /locus_tag="VNV63_02550"
     CDS             complement(549..1274)
                     /locus_tag="VNV63_02550"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HXC61533.1"
                     /translation="MPEVAGTTVSAPQSRAWRAAPESYYRARYYDPNSGRFLKEDPIG
                     FQGGGANFYAYTANSPLNFRDPSGLDYRTSYSSGKISVSLEIVLYGKGANPTLAALWQ
                     KAIADKWNQNPGYGRCKAVFDVSVSADPGANNIFSAQWRSYYGPKNWIRVPPNGQLGD
                     NGVNPITMNSGSWWSQLDLRTVAHEAGHLLGFWDHINPLTNAPWKGSENDIMAESGNY
                     AILQQEIDSIVGKWYDKECKCGQ"
BASE COUNT          352 a          396 c          372 g          367 t
ORIGIN      
        1 ctgccgaagg gatcgtattt gaagctatcg ggcgatgcgg actcggctct caagatacca
       61 accgttcctc cccaagacga tttccagagg acctgttttt ctgaaatcat ctcggacctc
      121 aaactcactc gtccctagca gaattctttc tgaatcgtgg ttttcaatcg agcggccatt
      181 gatcgctatt tttccatcgg aaacgtcaat gcggttacgc ccgaaatgga ttgttccgga
      241 gccagttacg accacgccag gcaacggtat agcggcttga aagtgaggaa cggccttaga
      301 ggtatgcttc agagaggctc cctcgaatcc taaatcttgc tcagatgaag gcggttcaag
      361 atagatcttc gccatcggca cgttgagatc ttgtggccca taggtctcaa tgatcacgaa
      421 actgtaggga actccgaagg ttctgggcag cttcgaaaat gcccatagag cggaagccgc
      481 ggataacaga ccgagaacta taaatagcac gaccagtgac tttgcaacgc gaatggaaac
      541 gcgctgtatc attgtccgca tttacattcc ttgtcgtacc attttccgac aatggaatcg
      601 atttcctgtt ggaggattgc atagtttccg gactctgcca tgatgtcgtt ctcagagcct
      661 ttccagggcg catttgtgag cgggttgatg tggtcccaga aaccgagaag gtgacctgct
      721 tcatgcgcaa ccgtcctcag atccagctga ctccaccaag atccggaatt cattgtgatg
      781 gggttaaccc cattgtcccc taactggcca tttggtggga ctctaatcca attcttgggg
      841 ccatagtaag acctccattg ggcagaaaat atgttgttcg ctcccggatc cgcgctcaca
      901 gaaacgtcaa agaccgcctt gcacctgccg tatccgggat tctgattcca cttgtctgca
      961 atcgctttct gccacaaggc ggccaatgtg ggattggcac ctttcccata caggacaatc
     1021 tccaaagaaa ccgagatctt accagaggaa taggacgttc tgtagtcgag tccggatgga
     1081 tcccggaagt ttaaggggga gttcgcggtg taggcataga agtttgcccc gccgccttgg
     1141 aatccgatcg gatcttcctt caaaaatctt ccggaatttg gatcgtagta tcgggcccgg
     1201 tagtacgatt ctggcgctgc tctccaggca cgggattgag gtgctgagac agttgtgccc
     1261 gccacttctg gcatgaagca taactcgaaa ccctgtactg cttcggcgtt ttatgcgcca
     1321 cccgccctgg ccagaaccga gtactacgag ctagcgctcg tcaggcgttc aggaaacgcc
     1381 ttctccacaa ctttgctctg taactttcca gtgccggcca acctgcttga gcatgacaat
     1441 ggcagtgttt ccgcataatt taccgcagga aaagctaaaa ctcatca
//
LOCUS       DATMKH010000211         2321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1698506, whole genome shotgun sequence.
ACCESSION   DATMKH010000211 DATMKH010000000
VERSION     DATMKH010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1698506"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..775
                     /locus_tag="VNV63_02555"
     CDS             <1..775
                     /locus_tag="VNV63_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HXC61534.1"
                     /translation="LFSHNPDSLLPWRLVFLLTLGYLGLVTGQKLAKELGGGITGPSS
                     SDAASASDKILDTSVIIDGRIADLCETGFLEGTFILPQFILQELQHIADSSDSMKRAR
                     GRRGLDILHRIQKMADIDVRVIDQDFPQIRDVDSKIVALSKKLNAKLITNDLNLNKVA
                     ELQGVRVLNINQLCNALKPVVLPGEMLRVFVLKEGKEAGQGVAYLDDGTMIVVDEARK
                     YIGKNMDVVVTSVLQTTAGRMIFTRLKEEPEREEVHLPR"
     gene            794..1561
                     /gene="ispD"
                     /locus_tag="VNV63_02560"
     CDS             794..1561
                     /gene="ispD"
                     /locus_tag="VNV63_02560"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HXC61535.1"
                     /translation="MKRGNGERLKVTAIVPAAGSGLRMKGNVPKQFLLLDGLPILAHT
                     LRALEPVPQIDEVILVVPESQISFCRTEIVNKYNLKKISKVIPGGEQRQDSVYKGLQA
                     VSADTDWVMVHDGVRPFVTQAMIESAIEAAKEAKDGAVVAIPMRDTPKEVLSNRITKT
                     LDRQRLWLAQTPQVFRRSLFLRAHEEARSQGIYGTDDAALVERLGGCVQIALGSEENI
                     KVTTPADLELAEAIIATRRTTGHLPAGRTGGPKKGSA"
     gene            1561..2049
                     /gene="ispF"
                     /locus_tag="VNV63_02565"
     CDS             1561..2049
                     /gene="ispF"
                     /locus_tag="VNV63_02565"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HXC61536.1"
                     /translation="MRVGIGYDAHPFAKDRRLVIGGIEIPFRLGLAGHSDADVLLHAI
                     SDALLGAMAEGDLGTFYPDTDPRYQGISSLKLLEEVYERVAKKGYRLANLDTVLIAEQ
                     PRLASYLPLMKETIAKVLKVKEQDISIKVKSNEQMGFVGRGEGIAAQAICALKKYEKK
                     KS"
     gene            2050..>2321
                     /locus_tag="VNV63_02570"
     CDS             2050..>2321
                     /locus_tag="VNV63_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HXC61537.1"
                     /translation="MLEKIRTDFKAVFDRDPAATKVSTVILTYAGFHAIVLHRLSHWF
                     WRQGVPLLPPLISYFSRFLTGIEIHPAAKIGKGFFIDHGMGVVIGET"
BASE COUNT          545 a          562 c          668 g          546 t
ORIGIN      
        1 tctattctct cacaatccag attctttatt accgtggcgc cttgtctttt tgctgacgct
       61 gggctacctg ggcctcgtta ccggacagaa gttggcgaag gagcttggcg gcggcataac
      121 cggccccagt tcttccgatg cagcttccgc ctcggataag attttggaca cgagcgtcat
      181 cattgatgga cggattgcgg atctgtgtga gacgggtttc ctggaaggga cctttattct
      241 tccgcagttt attcttcaag agctgcaaca catcgcggac tcatccgatt cgatgaagcg
      301 ggcccgcggc cgccgcggcc tggatattct ccaccggatt cagaagatgg cggatattga
      361 tgtacgtgtc atcgatcagg actttccaca gatacgggat gtagactcca agattgtggc
      421 gctgtccaag aaacttaatg ccaaattgat cacaaatgat ctcaatctca acaaggttgc
      481 cgaattgcaa ggtgttcggg tcttgaacat caatcagctc tgcaatgccc tcaagccggt
      541 ggtgcttccg ggtgaaatgc ttcgggtctt tgtcttaaaa gagggtaagg aggccgggca
      601 gggggtggcc tatctcgatg acggcacgat gatcgtcgtg gatgaggccc gcaaatacat
      661 tggtaaaaac atggatgtcg ttgtgaccag cgtacttcag accactgctg gccggatgat
      721 ctttacccgt ctcaaagaag aaccggaacg tgaagaggtg cacctgcctc gctgagtaga
      781 tcctgagcat ggaatgaaaa gaggaaacgg agagaggcta aaggtcacgg ccatcgttcc
      841 ggcagcaggc tcaggtctgc ggatgaaggg aaatgttccc aagcagttcc tactcttaga
      901 tggtctcccg attctcgccc atacgctgag agccctggag ccggtgcctc agattgatga
      961 ggtgatcttg gtcgttccag agtctcagat atccttctgc cggacagaga tcgtgaataa
     1021 gtacaatctt aaaaaaatct caaaggttat acccggtgga gaacagcgtc aagactcggt
     1081 ctacaagggt ctccaagccg tctcggccga caccgattgg gtgatggtcc atgatggagt
     1141 caggccattt gtgacacagg cgatgattga gtcggctatt gaagccgcca aagaggcgaa
     1201 ggacggtgcc gtggttgcaa tcccgatgcg ggatacgccc aaagaggttt tatcaaatcg
     1261 gattacaaag acactggatc gtcaaagatt atggcttgcc cagacccctc aagtttttcg
     1321 gagatctttg ttcttgaggg cccatgagga ggccagatcc cagggaatct atgggacgga
     1381 tgatgcggcc ttggtggaga gattgggagg atgcgtccag attgctttgg gctcggaaga
     1441 aaacattaaa gtaaccactc cggcggatct tgaactggcc gaagcgatta ttgcaacacg
     1501 tcgcacgaca ggccacctgc ctgccggacg aacaggtggc ccaaagaaag gttccgcatg
     1561 atccgggtcg ggatcggtta tgatgcccat ccttttgcca aggatcgtcg tttggtgatc
     1621 ggcggcatcg agattccatt tcgtcttggt cttgcgggac attcggatgc ggatgtcctg
     1681 ctccatgcga ttagcgatgc cctgctcgga gcgatggcgg aaggagattt ggggaccttc
     1741 tatccggaca cggatccccg ctatcaaggc atctccagct taaaactctt ggaggaggta
     1801 tacgagcgcg tggctaagaa aggttatcgc cttgccaacc tggataccgt tctgatcgcg
     1861 gagcagcccc ggcttgcatc gtatctcccg ttgatgaaag agaccatcgc gaaggtgtta
     1921 aaagtgaaag agcaagacat cagcatcaag gtcaaaagca atgagcaaat gggttttgtc
     1981 ggtcggggag aggggattgc ggcccaagcg atttgtgccc tcaagaagta cgaaaagaag
     2041 aaaagctgaa tgcttgaaaa aatccgaacc gattttaagg cggtctttga tcgtgacccg
     2101 gccgcgacga aagtcagcac ggtgattctg acctatgccg gtttccatgc gattgtgctt
     2161 catcgcttat cgcactggtt ctggcggcag ggtgtgccgc tcctgcctcc gcttatctct
     2221 tacttttctc gatttctcac cggcatcgag atccatccgg cggcgaagat cggcaagggt
     2281 ttttttattg accacgggat gggggtcgtc atcggcgaga c
//
LOCUS       DATMKH010000212         4694 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2332620, whole genome shotgun sequence.
ACCESSION   DATMKH010000212 DATMKH010000000
VERSION     DATMKH010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4694
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2332620"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="VNV63_02575"
     CDS             <1..473
                     /locus_tag="VNV63_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1646 family protein"
                     /protein_id="HXC61538.1"
                     /translation="CGHAPEGSQPELIEDHPENVKDVAIRACKVYLFVMALVLLGTGF
                     TPIVDKHLIHVPASVLYWINMVSAVLDNATLAAAEISPLMDLKKIQFLLLGLLISGGM
                     LIPGNIPNIICANKLSIKSKEWAVFGVPLGLCLMVLYFILMIFVRFQAGNAPEF"
     gene            750..3179
                     /locus_tag="VNV63_02580"
     CDS             750..3179
                     /locus_tag="VNV63_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HXC61539.1"
                     /translation="MFERFTDKGRKIIILAREEAERHQNDYLGTEHLVLAILRETDGI
                     ALTVLKKLGLSPEQVRLEIERNLPGGGTTMTFGEIPFTPRVKKVIEYSVEEARLLGHN
                     YIGSEHLLLGLLREEEGIGGKILRSLGANLLTARQLTINFLRRSTPREREKKSNTPAL
                     DEFGRDLTHLAVEGGLDPVIGRADEIERVLQILSRRTKNNPALIGESGVGKTAIVEGL
                     AQRIVASEVPDNLLNRRVIALDLGSLVAGTKYRGQFEERLKVVMKEIVQAGNIIIFID
                     ELHTLVGAGAAEGSIDASNMLKPALARGEIQCIGATTLDEYRKHIEKDGALKRRFQPV
                     YVQPPSVEETVMIIQGLRDRYEEHHNVEISQEAIVESVRLADRYVTDRFLPDKAIDVI
                     DEAGSRAKLQAFSLPVSLKGMEQELKKIIKEKELAITLQNFEEAVKFREEEERLRRLL
                     EESKREWKKNQEKNKPVISKEDVAYVVSKMTGIPLFKLEEEESQKLIHMEEGLHKRIV
                     GQDEAIAAVCRAIRRSRAGLKEAKRPIGSFIFLGPTGVGKTELARALAEFLFNDEDAL
                     IRIDMSEYMERFAASRLTGAPPGYVGYEEGGQLTEKVRRRPYSVVLFDEIEKAHPDIF
                     NLLLQVLDDGWLTDSLGRKVDFKNTVLIMTSNLGTRLVDHGSSLGFQKTAEKDAYGKM
                     KGDIMGELKRAFNPEFLNRVDEIVVFHSLEKKHLVTIVDMLIDEVNQRLALRGVQLEV
                     AQEVKDWLIQEGYQPAYGARPMRRAVQRNIEDPLSEDLIKGRFKDVKRIKAVLKGNTP
                     VFVEEEAMAPV"
     gene            3200..4012
                     /locus_tag="VNV63_02585"
     CDS             3200..4012
                     /locus_tag="VNV63_02585"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXC61540.1"
                     /translation="MSIVNEALKKAARQDTLERREEKQKSSFSTKTVFIQSFFFKILP
                     VLFLVLLLGYVATQRNLLVQWWSRFRPTPTVAKTAPAVASSTNVQSSLPKVNPSGTQV
                     STEAGAGQGIVSVQEEAVRRLKLGTQFFQNQKFKEAGEEFVRVVSLQPENAVAHNNLG
                     LALKAEGRAEDAETQYLMALKINPKYPEALNNLGLLYDQQGRVQEAIQQYRKAVDISP
                     DYPEAHLNYAQVLERAGYPEEARRHYQAFLTAGSATPSSLRLRIRQHLDTLP"
     gene            4100..>4694
                     /gene="tsaD"
                     /locus_tag="VNV63_02590"
     CDS             4100..>4694
                     /gene="tsaD"
                     /locus_tag="VNV63_02590"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004074584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HXC61541.1"
                     /translation="MLLLGIETSCDETAAAVVEDGQRLRSSVLSSQTELHHPYGGVVP
                     ELASRRHIEMVDRVVESALSQAGCRLKDLDAISVTCGPGLVGALLVGISFAKALAYSL
                     KIPLLAVNHLEGHIASVFLEPPEIIFPCIILVVSGGHTNLYYVKGYGQSRLLGQTRDD
                     AAGEAFDKAARMLGLGYPGGPVIDQLARLGDTTRIKFP"
BASE COUNT         1198 a         1104 c         1272 g         1120 t
ORIGIN      
        1 gttgcggtca cgctccagaa ggctcgcagc cagaactgat tgaagaccat cctgagaatg
       61 tcaaggatgt tgcgatccgt gcctgcaagg tctacctctt tgtaatggca ctcgtgctac
      121 tcggcaccgg ttttaccccc attgtagaca agcacctgat tcatgtgccg gcttcagtcc
      181 tgtactggat taatatggtg tctgccgttc tggacaatgc gacactggca gcagccgaaa
      241 tttctccgct tatggacttg aaaaagatcc agttcctcct gctcggcctt ctgatttcgg
      301 gcggcatgct cattcccggc aatattccca acattatctg cgccaataag ctctctatta
      361 aaagcaagga atgggctgtt ttcggagtac ccctgggtct atgtctgatg gtgctctatt
      421 ttatcctgat gatctttgtc cggtttcaag caggtaacgc tccagagttt taaagataag
      481 cgtgaaagct ggaaaacgca tccaaggcag caacgatgat gtttcatagc gatcgatgct
      541 ttcattcacc gtcgaaaaaa taatctcgtc accttagttt attgaatact gcttgacaat
      601 tcggaggcaa accgttacaa taaaaaccat gaaaacctta aaatattata taaagatctt
      661 agagaatgcc gagctaattc ttactaaatc cataagtcag aggagagttg ccgagactct
      721 aatcttctaa aaccctaaaa ggagaatgga tgttcgagcg atttacagat aaaggcagaa
      781 aaatcataat cttggcccgg gaagaagcag agaggcacca gaacgattac ctgggaacag
      841 agcatctggt gttggccatc ctgagggaga cagacgggat agccttgact gttttgaaga
      901 aattaggtct ctctcctgag caggttcgct tggaaatcga acgcaatctt cctggcggtg
      961 ggaccacgat gacctttggc gagattccct tcactcctcg ggtaaaaaag gtgatcgagt
     1021 attccgtgga ggaggccaga ctactggggc ataactatat tgggagtgag cacctccttt
     1081 taggactttt acgggaggaa gagggaatcg gtggtaagat cctgcgaagc ctgggtgcta
     1141 atctgctcac agcacggcaa ctgaccatta attttttacg gagatcaacg cctcgcgaaa
     1201 gagaaaagaa gagcaacaca ccggcgttgg acgaattcgg ccgtgatctt actcacttgg
     1261 cagttgaagg aggactggat ccggtgatcg ggagagcgga tgaaattgag cgtgtgcttc
     1321 agatcttgag ccgaaggacc aaaaataatc ccgccctaat cggggagtcc ggagtgggca
     1381 agaccgccat cgttgagggc cttgctcaga ggattgtggc ctcagaggtg cctgacaatc
     1441 ttctcaatcg ccgtgtgatt gccctggact tgggctcttt ggtggccggg accaagtatc
     1501 gcggccagtt cgaagagcga ctcaaggtcg tcatgaaaga gatcgttcag gcggggaaca
     1561 tcattatctt tattgatgaa ctccataccc tggtcggcgc tggtgcggcc gaaggttcga
     1621 ttgatgcctc caacatgttg aagcctgctt tagcgcgtgg cgagatacaa tgcatcgggg
     1681 cgacaaccct ggatgaatac cgaaagcata ttgagaagga cggcgcgctc aagcgtcgct
     1741 tccagcctgt ttatgtccag ccacccagcg ttgaagagac ggttatgatc attcaagggt
     1801 taagagatcg ctatgaagag catcacaacg tggagatcag ccaagaggcg atcgtggagt
     1861 ctgttcggct cgcagaccgc tacgtgacgg accgttttct tcctgacaag gcgatcgatg
     1921 tgattgatga agccggttct cgtgcaaaac tccaggcctt ctcgctgccg gtgtcattaa
     1981 aagggatgga acaggagctc aaaaagatca tcaaagagaa ggaacttgcc atcacgctcc
     2041 agaactttga agaggcggtc aagttccgtg aggaagaaga gcgacttcga cggcttctcg
     2101 aagaatccaa gcgggaatgg aagaaaaacc aggaaaaaaa taagccggtt atctccaaag
     2161 aggatgtcgc ctatgttgtc tctaagatga cgggcattcc gctctttaag ctggaagaag
     2221 aagagtccca gaaactgatc cacatggaag agggactgca caagcgtatc gtcggtcagg
     2281 atgaagcaat tgcggctgtc tgccgtgcaa tccgaagatc aagggccggc ctgaaagagg
     2341 ccaagcgacc gatcggctcc ttcatctttt taggacctac cggcgtcgga aaaactgagc
     2401 tggcccgggc cttggccgag ttcctgttta atgatgagga tgccttgatc cggattgata
     2461 tgtcggaata tatggagcga ttcgcggcat cgcggcttac cggcgctcct ccaggttatg
     2521 tggggtacga agaaggaggc cagctgaccg agaaagtaag acgtcggcca tattctgtcg
     2581 ttctctttga cgagatcgaa aaggcccatc cggatatctt caatctgctc ctccaggttc
     2641 tggatgatgg ctggttgacc gatagtctcg gccgcaaggt ggattttaaa aatacggtcc
     2701 tgatcatgac ctctaatctt ggtacgcggt tggtagacca tggttcctct ttgggcttcc
     2761 agaagacggc tgaaaaggat gcctacggga agatgaaagg tgatatcatg ggcgagctca
     2821 agcgtgcctt caatcctgag ttcctgaacc gggttgatga gatcgtggtc tttcattcct
     2881 tggagaagaa acatctggtc acgattgtgg atatgctgat tgatgaggtc aatcagcgtt
     2941 tggcattacg aggtgtgcag cttgaggtcg cacaagaggt aaaggactgg ctcatccagg
     3001 aaggctatca accggcctat ggtgcccgac cgatgagacg tgcggttcag cgtaacatcg
     3061 aggatccgct ttctgaagat ctgattaaag ggcgcttcaa ggatgtcaaa aggatcaagg
     3121 ctgtgttgaa aggcaatacc cccgtctttg tggaagagga ggctatggcc cctgtctgat
     3181 ccgctgtaga ggcggaagca tgagtattgt aaatgaagcc ttaaaaaagg cagccaggca
     3241 agacacccta gaacgacggg aagaaaaaca aaaatcaagt ttttcgacca aaaccgtctt
     3301 cattcaaagc tttttcttta aaatccttcc tgttttattc cttgtcctcc ttcttgggta
     3361 cgttgccacg caacggaatc tattggttca gtggtggtcc cggtttcgcc caacccctac
     3421 ggttgcaaaa acagcccctg cggtcgcgag ttccacgaat gtccaatcat ctctgcccaa
     3481 agtcaatccc tctggcactc aagtatcaac agaggcgggt gctgggcagg ggatcgtgtc
     3541 ggttcaagaa gaggcggtac gtaggctcaa gttggggacc caattttttc aaaaccaaaa
     3601 gtttaaagag gcgggggaag aattcgtaag agtggtatcc cttcagccag aaaatgccgt
     3661 ggctcataat aatcttgggc tggccttgaa agcggaggga cgtgcggaag acgcagagac
     3721 ccagtatttg atggcactca aaatcaatcc aaaatacccg gaggcattga acaacttggg
     3781 tctcctatat gatcagcaag gccgtgttca agaagccatt cagcagtacc gcaaggcggt
     3841 cgatatctct cccgattatc cggaagcaca cctcaactat gcccaagtcc tggagcgagc
     3901 cggatacccg gaagaggcac gacgacatta tcaggccttt ctgactgcag gttctgcgac
     3961 accttcctct cttcggctcc gcattcgtca acatttagac actttgcctt aaattaaggc
     4021 cactgctccg tagtcctctc tacccccatt gcgggaagag aggaagagta gggggtatag
     4081 atcgattgga agacaatgca tgttgctttt aggaattgaa acatcttgtg atgaaacagc
     4141 tgcggctgtg gtagaggacg gccaaagatt acgatcgtct gtcttatctt ctcagaccga
     4201 gctccatcat ccttacggag gcgtggtccc tgaactggcc agccgccgac atattgagat
     4261 ggtggatcgg gtagttgagt ctgcgctatc tcaagcaggt tgccgtctta aggacctgga
     4321 cgcgatcagt gtgacttgcg gtccgggtct tgtgggtgcg ctacttgtag ggatttcttt
     4381 tgccaaggcc ttggcttaca gtctaaagat tccgttgctc gccgtcaacc acctggaagg
     4441 gcatatcgca tctgtctttc ttgagccccc tgaaataatt tttccatgca tcattctcgt
     4501 ggtgtcaggc ggccatacga atctttatta tgtaaaggga tacggccaat cccgccttct
     4561 tggccagaca cgggatgatg cggctggaga ggcattcgat aaagcggcga ggatgctcgg
     4621 gctaggctat cctgggggac cggtgattga tcaactggcc cggctcggag acacgaccag
     4681 gatcaaattc cctc
//
LOCUS       DATMKH010000213         1454 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3608718, whole genome shotgun sequence.
ACCESSION   DATMKH010000213 DATMKH010000000
VERSION     DATMKH010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3608718"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="VNV63_02595"
     CDS             <1..440
                     /locus_tag="VNV63_02595"
                     /inference="COORDINATES: protein motif:HMM:NF026255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXC61542.1"
                     /translation="MSGGTYGAELFADWRVVPKWRLVGSYSYLQMNIQKNKDSLDPTP
                     DNPDGGSPRHQWYVRSSLDLPKHLEQDVTLRYVDRLPSLNIPSYYSLDAHVSWKPIAN
                     LEFSFGAQNLLNNQHVEFIPEFINTMPTRVARTFRGTITWRFE"
     gene            495..1082
                     /locus_tag="VNV63_02600"
     CDS             495..1082
                     /locus_tag="VNV63_02600"
                     /inference="COORDINATES: protein motif:HMM:NF025069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HXC61543.1"
                     /translation="MRKVGLLIAITCLLFSMAVGGHAEGLDSSDSSEYLIKAGFIYNF
                     AKLVEWPSTAFAQQDSPIVIGILGDDPFGGTLDRIVADKKINGRGLVVKRLRWGKDVK
                     DIRECNILFVSSSEKEHIDSVVDAMKWLPVLTIGDAPGFAKRGGIVNFTLEDNKVRFE
                     VNVEAAKRADLTISSRLLTLARIVQQVPADGKALQ"
     gene            1079..>1454
                     /locus_tag="VNV63_02605"
     CDS             1079..>1454
                     /locus_tag="VNV63_02605"
                     /inference="COORDINATES: protein motif:HMM:NF028461.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE sensor domain-containing protein"
                     /protein_id="HXC61544.1"
                     /translation="MNLKNLSIKRKLMLITMLTSGLAVVLSSAGFLSYDLISFRHSLS
                     QDLMTQAEIIGYNSAAAMAFKDESAATVTLSALQAKEDIVSAVLYTRDGKVFAQYIHA
                     NTRRPRLPAPPPERGYRFEGSYL"
BASE COUNT          358 a          358 c          385 g          353 t
ORIGIN      
        1 agatgagcgg aggaacttac ggcgcagaac tttttgcaga ttggagagtc gttcccaagt
       61 ggagactcgt gggttcctac agctatctgc agatgaacat acaaaagaac aaagatagtt
      121 tagacccgac tcccgacaat ccggatggtg ggagcccgcg ccatcaatgg tacgtgaggt
      181 cctcgttgga cctgcctaag catctggaac aggacgtgac ccttcgttac gtggatcggc
      241 ttccaagcct taatatccca agctactact ccctggatgc tcacgtcagc tggaagccaa
      301 ttgcaaacct cgagttctca tttggcgcgc aaaacctcct gaataatcag cacgtggagt
      361 ttattccgga atttatcaac acgatgccca ccagagtagc tcgcactttc cggggaacga
      421 ttacctggag atttgaatag tttggcgata gggctctcaa gattcggatt ccataagagg
      481 cgggctaggg tttcatgaga aaagttggat tgctgatcgc catcacctgt ctgctgttct
      541 ctatggccgt gggaggccat gcagaaggct tggactcctc tgattcttcg gaatatttaa
      601 ttaaggccgg ttttatttac aatttcgcaa agttggtgga atggccatcg actgctttcg
      661 cccaacaaga ttccccaatc gtaataggta tcttagggga cgatccattt ggtgggaccc
      721 tcgacaggat cgtcgcggac aagaaaatca atggacgagg cttggttgtc aaacggctga
      781 ggtggggcaa ggatgtcaag gatatcaggg agtgcaatat cctgtttgta agttcgtcgg
      841 aaaaagaaca catcgatagc gtggttgacg ccatgaaatg gctcccagtc ttgacaatcg
      901 gagacgcgcc tggtttcgcc aagcgtggcg gcatcgtgaa tttcactctc gaagacaata
      961 aagttcgttt tgaggtgaac gtcgaggcgg caaagcgcgc ggatttaacc atcagttcca
     1021 ggctgcttac gctggctaga atcgtccagc aagtcccggc tgatggaaag gcgcttcaat
     1081 gaatctgaag aatctttcca tcaagcggaa actgatgctg atcacgatgc tgacgagcgg
     1141 cctcgccgtt gttctgtcgt ccgcaggctt cctcagttac gatttaattt catttcgtca
     1201 ttccttgagt caggatctga tgactcaggc tgagatcatc ggatacaaca gcgcggctgc
     1261 gatggctttt aaagacgaat ccgctgcgac cgtaactctg tccgctctcc aggccaagga
     1321 agacatcgta tcggctgtgt tatatacccg cgatggaaaa gtcttcgccc aatacattca
     1381 cgcaaacacg cggcggcccc gccttcctgc ccctccgccg gaaagaggct accgttttga
     1441 aggaagctat ctgg
//
LOCUS       DATMKH010000214         7971 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2023064, whole genome shotgun sequence.
ACCESSION   DATMKH010000214 DATMKH010000000
VERSION     DATMKH010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 7971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7971
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2023064"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(166..561)
                     /locus_tag="VNV63_02610"
     CDS             complement(166..561)
                     /locus_tag="VNV63_02610"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXC61545.1"
                     /translation="MKNKHLLLGILAMCFLISCYGGPGSGGPPTSPAVNMALNAPKDF
                     SNKDAAAQNDEGVSHLQQGHWAVSTEHFQKAIATDPNLAEAHFNLALSLDEMGKHPEA
                     TEEFKKAKELAPNNPKIAENEVLKKHLGM"
     gene            complement(656..1648)
                     /locus_tag="VNV63_02615"
     CDS             complement(656..1648)
                     /locus_tag="VNV63_02615"
                     /inference="COORDINATES: protein motif:HMM:NF008218.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HXC61546.1"
                     /translation="MENSSFKPAWWCRGSHLQTVWRRLFGPTPAVATRHERLETPDDD
                     FIDLDWLMPGPDHRPLDGPIVLVLHGLEGSSLSKYVLGLLSELSRIHWQAVAMNFRSC
                     SGEINRQKHFYHSGETTDLDWVVGQLTQRFPAHPIYVVGFSLGGNVLLKWLGEEGEKV
                     PDSVRGAVAVSVPFDLEVAARRIDRGINRIYGANFLKTMKEKALEKTARYPGLFDPKA
                     ITRVRSFAAFDDLVTAPLHGFRDGLDYWLSSSSAQFLGEIRRPTLLISAEDDPFLPGK
                     YLPKKIVADSKWLESDFPAHGGHVGFVQGPMPWAASYWIDQRIVRFLKKQEGGL"
     gene            1748..2833
                     /gene="ald"
                     /locus_tag="VNV63_02620"
     CDS             1748..2833
                     /gene="ald"
                     /locus_tag="VNV63_02620"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HXC61547.1"
                     /translation="MIIGVSREIMDQEFRVALTPEGVKALVGAGHQVLVERGAGAGSG
                     FLDRGYREAGAKIVGSHAAVFKKAEMIVKVKQPLLSEYTLLQRGQILFTFLHLATNRR
                     LIDVLVRREVKAVAYETIEREDGFLPILKPMSEIAGRMSVLIGAFYLQKTFGGSGILI
                     SGIPEVEGAKVVILGAGTAGSNALQMAVGLGARVIVLDRDEKRVRQLRGVYGEHVIGL
                     VATQENIEAAILDADLSIGAVLLSGSRAPRLVSRKIVSRMKKGSVIVDISVDQGGCFE
                     TSRPTTHSHPVYEVKGVLHYCVANIPSVVPRTATLALTNATLPFMIEIANRGLEKAKA
                     ANPALAKGINIYDGKIVHPKVALALRM"
     gene            2860..2936
                     /locus_tag="VNV63_02625"
     tRNA            2860..2936
                     /locus_tag="VNV63_02625"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2894..2896,aa:Pro,seq:cgg)
     gene            2948..3427
                     /locus_tag="VNV63_02630"
     CDS             2948..3427
                     /locus_tag="VNV63_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00725 family protein"
                     /protein_id="HXC61548.1"
                     /translation="MKLPTVKRLQPTIGVIGGNQCGSKVAHQAEEVGRLIAQRGGIVV
                     CGGLGGVMEAVSRGASEAGGLCIGILPGGHSKEANPYIHIAIPTDMGHARNVIIARTA
                     DALIAIDGKFGTLSEVAFALILEKPVISLGSWEVDPKVLKAKNAKEAADLVFQAIGR"
     gene            3437..3721
                     /locus_tag="VNV63_02635"
     CDS             3437..3721
                     /locus_tag="VNV63_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HXC61549.1"
                     /translation="MGEEKFSAEIFVIGGVQGVGYRFFVEDAARELGLKGYCRNLADG
                     RVQIEAEGNRKGLEELIKRAWKGPGRARVTDVQATFRLAQGKFTDFTIQH"
     gene            3867..4793
                     /locus_tag="VNV63_02640"
     CDS             3867..4793
                     /locus_tag="VNV63_02640"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXC61550.1"
                     /translation="MSDESEEGSQDSLREDLPEEELSAEEQEDIPAETFDVVKSYLKD
                     IRKSALLTFEEEQELSKRMEMGDEKARQRMIESNLRLVVSIGKRYMNRGMAFSDIIEE
                     GNIGLIKAVERFNYRRGFRFSTYGSWWIKQAIERAIINQSKLIRLPVHVVEKLNHYLT
                     VNEQLIQELDREPTATELADKMEYSAEAVIGIQQLIRKTYSLDTPMGSNQDVALKDLI
                     VDTTQISPSINAEGVLRREIIFQWLDTLKESERKVVILRFGLEGEEPQTLEEIGKVFG
                     LTRERMRQIEGVALNKLRALIEKKTIKPEEIL"
     gene            4793..5320
                     /locus_tag="VNV63_02645"
     CDS             4793..5320
                     /locus_tag="VNV63_02645"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HXC61551.1"
                     /translation="MNPTALNLKSKIREIPDFPKEGILFYDITTLLKDSAAFSAVFKK
                     LGSHYKDSGIQKVASIEARGFIFGAPLALQLGAGFVPIRKTGKLPADVYESTYSLEYG
                     KSAIAVHQDAITAGERILLVDDLLATGGTMAAAVDLIKRLGGVIHGLAFLVELTDLKG
                     RDKLKGYDVLSLLQY"
     gene            5384..7552
                     /locus_tag="VNV63_02650"
     CDS             5384..7552
                     /locus_tag="VNV63_02650"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HXC61552.1"
                     /translation="MEPLKIAFLWHHHQPYYKDPLSGEYSLPWVRLHAIKAYYDMAGL
                     LKDFPEIKAGVNLVPSLLKQLQDYEQGTVDQFLERTLKPAEDLETEERRFLLRYFFMA
                     NWETMVKPWPRYYELLQRRGLRWDGEGGERMLTQFTPKDYRDLQVLFNLAWFGFKAME
                     EFPVLQEFRRKGEGFTEEDKAEVIRCQHEVLHRVIPLYRQLESSRQLELTTSPFYHPI
                     LPLVYDTDIARRCMPHAPLPDRFQAPEDARAQILRAVEFHAKLFGRKPTGFWPSEGSV
                     CPEIIPLLADAGIRWIATDEEILLNSMPTQSRTEILYRPYLTGSDGREVSIVFRDKDL
                     SNLISFTMGNMDPRAAVDEFFKRFHSIQNAVSDRKGPSLATIILDGENPWENYPDSGR
                     LFLTELYTRLSKSEAFQTVRIMDEIERHPPHQRIHHLYSGSWIHHDFDIWIGSDEENR
                     AWSYLSRTRKALLPLLNDASVPAAQREAAWEAIYAAEGSDWFWWYGDDFSSSNDEEFD
                     RLFRAHLASAFRHLGQEAPEYLSRPIIHLHPYKHVTEPVNFIHPTLDGKRTTYFEWQG
                     AGFYDATRRSTRFGEERLLSAICYGFNLTHCYIRIDPDEGCGPEKRKRYEIHVHFFGG
                     PAEYRWVIHCALEPLRLTVERSPDGIRYEPVGEVNTVVMGTILELAVPFAMLGWKPGE
                     RHNFIVEIREQGSVVETYPPNGYLTLEVPDQDFEQLMWSV"
     gene            7552..>7971
                     /locus_tag="VNV63_02655"
     CDS             7552..>7971
                     /locus_tag="VNV63_02655"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61553.1"
                     /translation="MGQVSLLLGIHNHQPVGNFEHVFREAHDRCYRPFLDLIEGHKNL
                     RISLHYSGPLLDWLEKHEPSFLDRLRKCVEHGQVQLLAGGYYEPILPAIPERDGIGQI
                     TMLIQHLKRRLGANSHGLWLTERVWEPSLPSMLGACGV"
BASE COUNT         1921 a         1987 c         2251 g         1812 t
ORIGIN      
        1 ttcctcaaaa tcgcgctgat tcccataggc taagagcaga tgaccgcacc catcgcagat
       61 tgccacatcc cggtgttttt tcaaataagc caggatagat gaatggcaac acggcttcat
      121 tacagcttta tagagcggtc ataatatagt gccacagggc cggacttaca tccccaggtg
      181 ctttttcaag acctcatttt cagcgatctt tggattgttc ggagccagct ctttggcttt
      241 tttaaactcc tcggtcgcct cggggtgttt ccccatctca tctaaagaca acgcgagatt
      301 gaagtgagct tccgctaaat tgggatccgt ggcaatagcc ttttgaaaat gctctgtcga
      361 tacagcccaa tgcccctgtt ggagatgact caccccttca tcattctgtg cggccgcgtc
      421 tttattggaa aaatcttttg gggcgttcag ggccatgttg accgctggac ttgtcggagg
      481 accgccgcta cccgggcccc cataacacga gatcaagaag cacattgcca gaataccgag
      541 aagcaaatgt ttgtttttca tcagacgcct ccttatttat tttttaagtg aatgagttct
      601 tgaaaggatt tctacaatac catttagaaa ataggctgtc aacaggtgga gacggtcata
      661 aaccgccctc ctgtttttta agaaaccgga cgattcgctg atcaatccag tatgaagcag
      721 cccatggcat cgggccttgg acaaaaccca catggccgcc atgggcggga aaatcagact
      781 caagccattt cgagtcagcg acaatctttt ttggaagata ctttccggga agaaacggat
      841 cgtcctcagc gctgatcaga agggtcgggc gccgtatctc gccgagaaac tgggcggagc
      901 tggagcttag ccagtaatcg aggccatccc gaaacccgtg taagggtgcg gtgacaagat
      961 catcaaaggc ggcgaaggaa cgaacccgag ttatcgcttt ggggtcaaac agtcctggat
     1021 agcgggctgt tttttccagc gctttctctt tcattgtctt taagaagtta gccccataga
     1081 tccggttaat gccccgatct atccggcggg ccgccacttc cagatcaaac ggtaccgata
     1141 ctgccactgc tccacgtacc gaatccggaa ccttctcccc ttcttcaccc agccacttga
     1201 gcaggacatt tccgccgaga gagaaaccta ccacatagat cggatgggcc ggaaacctct
     1261 gtgtcagctg tccgacgacc cagtccagat cggtcgtctc tccggaatga taaaaatgtt
     1321 tttgacggtt gatctcaccg ctgcaggacc tgaaattcat cgcaaccgcc tgccaatgga
     1381 tgcgactcaa ctcggagaga agacccagaa catatttact cagggaggac ccttcaaggc
     1441 catgcaaaac cagaacgatc ggtccatcga gaggacgatg atcgggaccc ggcatcagcc
     1501 agtccaagtc aataaaatcg tcatccggcg tttccaatcg ctcatgccgg gtcgccacgg
     1561 ccggagtcgg accgaagagc cgccgccata cagtctggag atgcgatccg cggcaccacc
     1621 acgcgggctt gaaagaagaa ttttccataa gcggaaacca attatagcgg ttctttataa
     1681 agagatcaac gtttcattga ctttcgaaaa ggaaactgtt atcaataacc ggcgaatgag
     1741 gagaaagatg atcatcggcg tttcaagaga gatcatggat caggaatttc gtgtggcctt
     1801 aacgcctgaa ggcgtcaaag ctttagtggg cgcgggccac caggtgctcg tcgagcgggg
     1861 cgccggtgcg ggcagcggct ttttagaccg tgggtatcgg gaagcgggcg ccaaaatcgt
     1921 cggatcgcac gcggcggttt ttaaaaaagc ggagatgatc gtcaaggtca agcaaccatt
     1981 gctttccgaa tatacgcttc tccaacgcgg ccagatcctg tttaccttcc tccatctagc
     2041 tacgaatcgg cgattgattg atgtcctggt ccggcgagag gtcaaggccg ttgcgtacga
     2101 gaccatcgaa agagaggacg gtttcttgcc gatcttaaaa ccgatgagcg agatcgctgg
     2161 ccggatgtct gtgttgatcg gtgccttcta tcttcagaag acctttggag gttccgggat
     2221 tcttatttca ggaattccgg aggtggaggg cgccaaggtc gtgatactgg gtgcggggac
     2281 cgcgggatca aacgctcttc aaatggctgt gggtttaggg gcccgcgtga ttgtactgga
     2341 tcgggatgaa aaaagagtgc ggcaactccg tggtgtgtat ggggagcatg tgatcggact
     2401 ggttgcaaca caagaaaata tcgaggccgc gatcttagat gctgatctat ccatcggtgc
     2461 ggtgctcctc tcaggatcaa gagcgcctcg attagtatct cgaaaaatcg tgtctcgaat
     2521 gaaaaaagga tctgtcattg tggatatctc ggtggatcag ggaggctgtt ttgagacgag
     2581 ccgccccacg acgcattctc atcccgtgta tgaagtaaaa ggggttcttc actattgcgt
     2641 cgccaacata cccagcgtcg ttccgcgaac agccactttg gccttgacca atgccacact
     2701 tccttttatg atagagattg ccaaccgggg gcttgagaag gccaaagcgg ctaatcccgc
     2761 cttggccaag ggcatcaata tctatgacgg caagattgtt catccgaagg tggctttagc
     2821 tttaaggatg taatattcta gctgtaaaca acaatatgtc ggggcgtagc gcagcctggt
     2881 agcgcacctg cttcgggagc agggggccgc tggttcaaat ccagtcgccc cgaccatatc
     2941 caaatagatg aaactgccaa ccgttaaacg gcttcaaccc acgatcggtg ttatcggcgg
     3001 gaaccagtgc ggctcgaaag tggcacatca ggccgaagag gttggacgat tgattgccca
     3061 acggggaggc atcgtggtgt gcggcgggct cggcggcgtc atggaggcag tgtcccgggg
     3121 cgcttcggag gcgggcggtc tctgcatcgg tatcttgccg ggagggcatt cgaaagaggc
     3181 gaatccctat atccatatcg cgatcccaac cgacatgggc cacgcgcgca acgtgatcat
     3241 tgcccgaacc gcggatgcgc tgattgcgat tgacgggaaa ttcggcacgc tttcggaagt
     3301 ggcttttgcg ctgattttgg aaaaacccgt gatcagtctg ggctcctggg aggttgaccc
     3361 aaaggtgttg aaggcgaaga atgcgaaaga ggcggcagat ctcgttttcc aggccatcgg
     3421 tcgttagcgt tgaggaatgg gagaagaaaa attttctgcg gagatctttg tgatcggagg
     3481 ggtacaggga gtcggctatc gtttttttgt ggaagatgct gcccgggagt taggcctcaa
     3541 aggttactgc cgcaatctcg cggatggacg agtgcagatc gaagcagagg ggaatcgaaa
     3601 gggtcttgaa gaattgatca aaagagcctg gaaaggtccg ggccgtgcca gggtgacaga
     3661 tgtccaggcc acattcaggc tggctcaggg aaaatttaca gacttcacga ttcagcattg
     3721 attatagtga gcaaggcgag cgagagggga ggctccaagg gctttgcata ttggaggggg
     3781 cgacgcaagc ccctatgatc atgtaaaaaa tgcttttatc acacaataaa ttcaagtata
     3841 ataggcaaaa tttaagtagg tgaacgatgt cggatgagtc agaagaagga tcgcaggata
     3901 gtttaagaga ggatctcccc gaagaagagc tttctgccga agagcaggaa gacatcccgg
     3961 ccgaaacctt tgatgtggtc aaaagctact taaaggatat acgcaagtcc gccctgttga
     4021 cctttgagga agagcaggaa ctgtccaaac gaatggagat gggggacgag aaggcccgcc
     4081 agcggatgat cgaatccaat ctgcgcctgg tagtgagcat cggaaagcgg tatatgaatc
     4141 ggggaatggc tttctccgat atcatcgaag aaggtaatat cggattgatt aaagcggttg
     4201 aacggtttaa ttaccgacga gggttcaggt tcagcaccta cggttcttgg tggatcaaac
     4261 aagccattga gcgggccatc attaatcaaa gcaagttgat tcgattgccg gtgcatgtcg
     4321 tggagaagct gaatcattat cttaccgtca atgagcaatt gatacaggaa cttgatcggg
     4381 agccgaccgc cacagaactc gcggacaaga tggagtacag cgcggaagcg gtcatcggga
     4441 tccaacaact tattcgaaag acctactccc tggacacccc gatgggcagc aatcaggacg
     4501 tggcactcaa ggatctgatt gtggacacga cccagatctc accttcgatc aatgcggagg
     4561 gggtcctccg gcgggagatc atcttccagt ggttggatac cttgaaagaa tccgagcgga
     4621 aggtagtcat tctgagattt gggttggaag gcgaagagcc tcaaacgctg gaggagattg
     4681 gaaaggtctt cggcctgact agagaacgga tgagacagat cgagggcgtg gctttgaaca
     4741 aattacgtgc cctaatcgag aagaaaacga tcaaaccgga ggagattctg taatgaaccc
     4801 aacggccctt aatctcaaat caaagatccg cgagataccc gattttccca aggaaggtat
     4861 tctgttctac gacatcacga cgcttctcaa agactcggcc gccttttcag cggtttttaa
     4921 aaaattgggt tcccattaca aagactccgg tatccagaaa gtggcctcca tcgaggcccg
     4981 gggttttatc ttcggcgcgc cgctcgcgct tcagctcggg gcagggtttg taccgatccg
     5041 caaaacgggg aagttgccgg ccgatgtgta tgagtccacc tattcgctcg agtacggcaa
     5101 aagcgcgatc gccgtgcatc aagatgcgat cactgcgggt gaacggattc tcctggtgga
     5161 tgatcttctg gcgaccggcg gcacgatggc cgccgctgtg gatttgatca aacgcctcgg
     5221 gggagtgatt cacgggctgg cttttctggt tgaactcacc gatctgaagg gacgcgataa
     5281 attaaagggc tatgacgtcc tgtcccttct tcaatattag ccttgttaga cttttctcac
     5341 ctctttgttt gtgctaatat agctaacaga ctcggaaggt cctatggaac ctcttaagat
     5401 cgcctttctc tggcatcacc accaaccata ttataaagac ccattaagcg gcgagtattc
     5461 cctgccctgg gtaaggctgc acgcgatcaa ggcgtattac gacatggcgg gcctcctgaa
     5521 ggatttcccg gagattaaag caggcgtaaa cctggtgccg tccttgctca agcagcttca
     5581 ggactacgag caggggacag tggatcagtt ccttgagcgg acactgaagc ccgcggaaga
     5641 ccttgaaacg gaagagcggc gattcctatt acggtatttc ttcatggcca actgggagac
     5701 gatggtgaaa ccctggccga ggtattatga acttctccaa cgcagagggc ttcggtggga
     5761 cggcgaaggc ggggagcgga tgcttactca atttacgccg aaagattatc gggacctcca
     5821 ggtccttttc aacctggcct ggttcggttt taaagcgatg gaagaattcc cggttcttca
     5881 ggaattccgg cgaaagggcg aaggatttac ggaagaagat aaggccgagg tgatccgatg
     5941 ccagcacgaa gtactccatc gcgtcatccc gctttaccgg cagctggaat ccagccgtca
     6001 gcttgaattg accaccagcc cgttctacca tcccattctg ccgttggtgt acgacacgga
     6061 catcgcgcgt cggtgcatgc cccatgcccc gctcccggat cgcttccagg cgccggagga
     6121 cgcgcgggca caaatcttgc gcgcggttga gttccatgcc aagttgttcg gccgaaaacc
     6181 gacgggtttc tggccgtcgg agggatcggt ctgcccggaa atcatcccgc ttctggcgga
     6241 tgcgggtatc cgttggatcg caacggacga agagatactg ctcaattcca tgccaacgca
     6301 gtcccggaca gagattctct atcggcctta cctgaccgga tccgatggac gggaggtatc
     6361 gatcgtattt cgggataagg atctgtccaa cctgatcagc tttacgatgg gaaacatgga
     6421 cccacgagcc gccgtggatg aattcttcaa acggtttcat tctattcaga acgcggtgtc
     6481 cgaccgcaag ggaccgtctc ttgcgacgat tatactggac ggcgagaatc cgtgggagaa
     6541 ttacccggac agcggacgtc tttttctgac cgagttgtat acacgtcttt caaaatccga
     6601 ggccttccaa acagtccgga tcatggatga gattgaacgg catcctcccc atcagcggat
     6661 tcaccacctc tactccggtt cctggatcca tcatgatttc gacatctgga tcggatccga
     6721 tgaggaaaat cgggcgtgga gctatttgag ccggacgcgc aaggcgctcc tgcccctttt
     6781 aaacgatgct tccgttccgg ctgcgcagcg tgaggcggcg tgggaagcga tctatgccgc
     6841 cgaggggagc gactggttct ggtggtacgg ggatgatttt tcaagctcga atgatgagga
     6901 attcgaccgg ctgttcaggg cacacttggc aagcgctttc cggcatctcg gccaggaagc
     6961 tcccgaatat ctatcacgtc ccatcattca ccttcatcct tacaagcatg tgaccgaacc
     7021 ggttaatttc atccatccga cgttagatgg gaaacgcacc acctattttg agtggcaagg
     7081 ggcgggcttt tatgatgcga cgcgtcgcag cacacggttc ggcgaggagc gccttctgtc
     7141 ggccatctgt tacggattca atctaacgca ttgctatatc cggattgatc cggacgaagg
     7201 ctgcggtccg gagaaaagaa aacggtatga gatccatgtc catttcttcg gagggccggc
     7261 cgagtaccga tgggtgatac actgtgccct ggagccgcta cggttaactg tggagcggag
     7321 ccccgatggg attcgatacg aaccggtcgg agaggtgaac accgtagtga tgggaacgat
     7381 actggaactg gccgtgccgt ttgcgatgct gggatggaag cccggagagc ggcacaattt
     7441 tatcgttgag atccgtgagc agggaagtgt ggttgaaacc tatcctccaa acggttatct
     7501 cacgctggaa gtgccggatc aggatttcga acaactgatg tggagtgtct gatgggacag
     7561 gtctccctgc ttctcggaat tcacaaccat caaccggtgg gcaattttga acatgtgttc
     7621 cgggaggcgc atgaccggtg ctaccggccg tttcttgatc tgatcgaagg gcacaagaac
     7681 ctccgtatct cgctgcacta cagcggccct ttgctcgatt ggctggaaaa acacgaacct
     7741 tcatttctgg atcggctccg gaaatgtgtg gaacatggac aggtgcaact cctcgccggc
     7801 ggctattatg agcctattct cccagcgatc cctgagcgcg acgggatcgg acagattacg
     7861 atgttgatcc agcacctgaa gcggcgtctg ggagcaaatt ctcacggcct ctggctgacc
     7921 gaacgtgtgt gggaaccttc tcttccttct atgctgggtg cttgcggtgt g
//
LOCUS       DATMKH010000215         2789 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2987224, whole genome shotgun sequence.
ACCESSION   DATMKH010000215 DATMKH010000000
VERSION     DATMKH010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2987224"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..1221)
                     /locus_tag="VNV63_02660"
     CDS             complement(40..1221)
                     /locus_tag="VNV63_02660"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXC61554.1"
                     /translation="MISWNAILAIAYKEFVHVWRDRRVMLLIVVLPPFFTFLFGHAFA
                     NSTIQDVPAMYFDADNTEQSQAFLALLKGKDIFDWRPWNGDPEGKIELFPAGVEAAIV
                     IPKGWGQSLRNGDPKPIRGVLDGTDTNTAPSVQGALQETLGDYQGKQRDDLISNLPDE
                     VIDLGKQLPPEIRHEFSSMMTPWDVKIETLYNPNLLFIDYVVPGIVGLILQLITVTLI
                     ASTITREREVGTLSQLMVTPLRQTEIVIGKVLPYLVISMFLIASTIAVGSLHFGVKFQ
                     QPLLLSITCFLFLLFSLGLGLLISAFSRTQTQAIQISIFFLLPVMLLSGAFAPLSQLP
                     RPVRIVSEFFPLTHFCRAFRNVNLHHSHITAIAGDLAFLAVGAIVTCAGASYLLRMID
                     E"
     gene            complement(1218..2192)
                     /locus_tag="VNV63_02665"
     CDS             complement(1218..2192)
                     /locus_tag="VNV63_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXC61555.1"
                     /translation="MPESLIVLKNLSKRFGKKKAVNRISISIQRGEIFGFLGANGAGK
                     TTTIRMLCGLTKPTAGLGTIGDLNIWKDRFRIRSRFGYVPQKFSLYSDLTVGENLRFF
                     AGAYRVPGGKIRDRTHKVLCDMDLEERESERAGSLSGGYKQLLAIACALIHEPTLLFL
                     DEPTAGLDPTHRQAIWDLIYELSQAGTTIFVTTHYMDEAERCTDVGFIDGGRLIAKGS
                     PRDLKEGLKGHLLEVQVEPAMTAMFELRKLPGVFGADLRSGNLRLQAEDPDALLHSWQ
                     IHWPFPNLKFLGFNWVEPDMEDVFQAYSRGYYRPERDQVEAGQLAYKL"
     gene            complement(2262..>2789)
                     /locus_tag="VNV63_02670"
     CDS             complement(2262..>2789)
                     /locus_tag="VNV63_02670"
                     /inference="COORDINATES: protein motif:HMM:NF039394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6268 family outer membrane beta-barrel
                     protein"
                     /protein_id="HXC61556.1"
                     /translation="AHPGFYSESGNISWRDFNVPFEVAATYFVSSDLLIIGGVYVDVN
                     NDIPAFPVFGVRWKVSDKWVIEGIPPRPQLQYLLSDSVTLFAGADLREQSFRMDEAFG
                     QERNIPKLNDAIVNYFEVRGGAGLTWKISKNVNLEVEGGGTPYRRFYYPRADGFKVKS
                     EDLVPYLRVGLSAKF"
BASE COUNT          712 a          776 c          699 g          602 t
ORIGIN      
        1 agagaatcaa ggacgagaac taagacaatt caatctaatc tactcgtcga tcatcctcaa
       61 aaggtaggag gcacccgcgc aggtgacaat tgcgcccacg gcgagaaaag ccaggtcgcc
      121 ggcgatcgcg gtgatatggc tgtgatggag attcacattc cgaaaagccc ggcaaaaatg
      181 cgtcagagga aaaaactctg aaacaatgcg gaccggtctc ggaagctggc tcaacggggc
      241 aaaggcaccg gacaacagca tgacgggcaa gagaaagaaa attgaaatct gaatggcctg
      301 ggtctgagtg cgcgagaagg ctgaaatcag taaaccaagg cccagcgaga ataagagaaa
      361 caaaaaacag gtgatcgaca gcaacaacgg ctgttgaaat ttcaccccaa aatgcaaact
      421 gcccaccgcg atggtgctgg cgatcaagaa catcgaaatc accagatagg gcagcacctt
      481 gccaatgacg atctcagtct ggcgcaacgg cgtaaccatt aattgggata atgttcccac
      541 ttctcgttct cgcgtgatcg tgctggcaat caacgttacc gtgatcaact gcagaatgag
      601 gccgacaatg cccgggacta cgtaatcgat aaataacaaa tttggattgt acagggtctc
      661 gattttgacg tcccaagggg tcatcatcga actgaattcg tgcctgattt ctggcgggag
      721 ctgctttccg aggtcgatca cttcatccgg tagattcgag atgagatcat ccctctgttt
      781 gccctggtag tcgccgagtg tttcctgaag ggctccctga accgaaggtg cggtgttcgt
      841 gtccgttccg tccaggactc cgcggatcgg tttcggatca ccgttccgca aactttgacc
      901 ccagcccttc ggaatcacga tggccgcttc gacgccggcc gggaaaagct cgatcttccc
      961 ttccgggtcg ccgttccatg gtctccaatc aaaaatgtct ttccctttaa ggagagccag
     1021 gaaagcctgg ctttgctcgg tattgtcggc atcgaaatac atcgccggca catcttggat
     1081 ggtcgaattc gcaaaggcgt gcccgaaaag aaaggtaaaa aacggcggca acaccacgat
     1141 caacaacatc acgcgcctat cgcgccagac atgaacaaac tctttatagg cgatggccag
     1201 gatagcgttc caggaaatca taatttgtag gccagttgcc ccgcttccac ctgatcccgc
     1261 tccggtcggt agtaacctcg ggaataagcc tggaagacat cctccatatc cggttcgacc
     1321 cagttaaacc ccaaaaattt taagttagga aacggccaat ggatctgcca tgaatgcagc
     1381 agagcatccg gatcctcagc ctggagacgc aaatttccgc tccggagatc tgctccaaat
     1441 accccgggca atttgcggag ctcaaacatc gcagtcatcg ccggttcgac ctgtacctcc
     1501 aggaggtgtc ctttgagccc ttctttcaga tcacgcgggg atcccttcgc gatcaggcgg
     1561 cctccatcga tgaatccgac atccgtgcaa cgttcagcct cgtccatgta gtgtgtcgtc
     1621 acgaagatcg ttgttccggc ctggctcagt tcgtagatca aatcccagat cgcctggcga
     1681 tgcgtcggat ccaggccggc agttggttca tccagaaaaa gcagcgtcgg ctcgtgaatc
     1741 agcgcgcaag ctatcgccag aagctgttta tacccgccgg acaagcttcc ggctctttct
     1801 gattcgcgct cttccaggtc catgtcgcaa agaaccttgt gcgtccgatc tcttattttg
     1861 ccgccgggaa cccggtacgc gccggcaaaa aaacgaaggt tctcccccac cgtgagatcc
     1921 gagtaaaggc tgaacttttg cggcacgtat ccgaaacgcg agcggattct aaatcgatct
     1981 ttccagatat tcagatctcc gatcgtacca aggccggccg tcggcttggt caacccacaa
     2041 agcatgcgaa tcgtcgttgt tttacctgca ccgttagcac ccagaaaacc gaagatctca
     2101 ccccgctgga tcgaaatcga aattctgttg acggcttttt ttttgccaaa cctcttcgag
     2161 aggttcttca ggacgatgag cgattcaggc atgaaattgc aaaaacgacc ccgggctctt
     2221 ggagtcctct ggagatggat acctgagaat tcctgccgag gtcaaaactt tgctgagagt
     2281 ccgactcgaa gatacggcac cagatcctca gatttgactt tgaatccatc cgctcgcgga
     2341 tagtagaagc gccgatacgg agtgccgcct ccttccacct cgaggttgac attcttggag
     2401 attttccagg ttaagccggc accgccccga acttcaaaat agttcacgat cgcgtcattg
     2461 agcttcggaa tgttccgctc ctgtccaaac gcttcatcca ttcggaacga ctgctcgcgc
     2521 aaatcagcgc cggcgaacaa cgtcacgctg tccgacagga gatactgcag ttgcgggcgt
     2581 gggggaattc cctcgatcac ccatttgtcc gaaactttcc agcggactcc aaagaccgga
     2641 aatgccggaa tatcattgtt cacatcgacg tacactcctc cgatgatcag gagatccgaa
     2701 ctcacaaaat acgtggcggc aacttcgaaa ggtacattga aatcccgcca ggaaatattt
     2761 ccagactcgc tgtagaaccc aggatgcgc
//
LOCUS       DATMKH010000216         1199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3609704, whole genome shotgun sequence.
ACCESSION   DATMKH010000216 DATMKH010000000
VERSION     DATMKH010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3609704"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="VNV63_02675"
     CDS             <1..734
                     /locus_tag="VNV63_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61557.1"
                     /translation="MSWPCFVTYVLATDRSLAPPGYVHQVRPGQKCYLCSVPSGQKHP
                     HSPTRQRTKNMGNSVEQTFDEFLRTNDLCSWDRNYMKWYGSIVIKPAGLPDPFSFTLV
                     VYLTDAHEKRSGICHVLEWSQERQSLCVTKSVHCAEPLHEDNRLKGMDKARNDLLFLQ
                     EPTDSVPSLLVETFLDDHFRSQICQNLQDPEQRLPILHWLQDQQFSVYRFLSGPEGSR
                     NKFQKAMFPLKPDCEKYYRRGEHKE"
     gene            1001..>1199
                     /locus_tag="VNV63_02680"
     CDS             1001..>1199
                     /locus_tag="VNV63_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HXC61558.1"
                     /translation="MRTITLEEHFATAGFLDGPGRDLKARAQQYGDRAAKLLEQLCDL
                     GDKRIAEMDAAGIDVQVLSLTS"
BASE COUNT          293 a          327 c          305 g          274 t
ORIGIN      
        1 ctatgtcttg gccatgtttt gttacctatg tcttggccac ggaccggagc ctcgccccac
       61 caggctacgt tcatcaagtc cgtccgggac agaagtgcta tctttgttca gtcccttcgg
      121 gacaaaaaca cccccactcc cctacccgtc aacgaactaa aaatatgggc aattcagtag
      181 aacaaacctt cgatgaattt ctcagaacaa atgatctgtg ctcatgggat cgcaactaca
      241 tgaagtggta cggctccatc gtgattaaac ctgccggctt gcccgatccg ttttcattta
      301 ctctcgttgt gtatctgaca gatgctcacg aaaagcggtc cgggatctgt cacgttttgg
      361 agtggagcca agaacggcaa agcctctgtg tcaccaagag tgtgcattgt gccgagccac
      421 ttcatgaaga caaccgttta aagggcatgg acaaagccag gaatgacctt ttgttcttac
      481 aagagccaac tgactcggtt ccatcgctgc tagtcgagac gttcctcgat gatcattttc
      541 gatcacagat ctgccagaac ctgcaggacc ctgaacagcg cctgccgatc ttgcactggc
      601 ttcaagatca acaattctcg gtctaccgct tcctctccgg tccggaaggt tcccgtaaca
      661 agttccagaa agccatgttt ccgttgaaac cagattgtga gaaatattat cggcgcggag
      721 aacataaaga gtaggaactg aagatggagt cgcccgccgt ggagcgttgc ctcgcttgcg
      781 aggccgaaag aggtggccac ggtcgaccgt cttacctatt gcaccattcg ttcggtaggg
      841 acgagctccc gctcgtccgg gaattagctc agacaaaatg aaggcgtgga agaagggaaa
      901 atccggacga gcgggagctc gtccctaccg atgacctctt caccaagcca acgagtgacg
      961 acccagttgc actggtgtct tcagtcatcg aagaacaatc atgcgaacca tcacgctgga
     1021 agaacatttt gcgaccgccg gattcttgga tggcccgggg cgagacctca aggctcgagc
     1081 ccaacaatac ggcgaccgtg ccgcgaagct tttggaacag ctctgtgatc ttggtgacaa
     1141 gcggatcgca gagatggacg ccgcgggtat tgatgtccag gttttatcgc tcacttctc
//
LOCUS       DATMKH010000217         1755 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_741008,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000217 DATMKH010000000
VERSION     DATMKH010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_741008"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..>1755)
                     /locus_tag="VNV63_02685"
     CDS             complement(42..>1755)
                     /locus_tag="VNV63_02685"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXC61559.1"
                     /translation="LEVIVSDSRQWVLGGEAMKESAGRMDSALSLALAGKEEEGRKLF
                     ESLAPVFERFLGGKGASVWLPGEGEVADRIKQAEEHYQDLAGKFWNTSEPGARRTMYT
                     AELLPLFTEIKNELEEVVAVNDVNTVQVGRQARTLSTNSTGTMFVVWAIGVGIVVSVY
                     FATRRAIFKPIGILTAVAKELGNGNLDQVVPVQSNDEIGALAAAFNKLSAKLRAYRQI
                     TADQILQARQMTEITFSAFPDAILALSGDARIDFANPAAEELLRGLDSKVGLPLRIRE
                     EAEKVLKGATDYLPTSFEEAVAVRVNEHEVYLLPRVIGMRDESGKLFGAAIVLQDVTR
                     FRLMDEVKTNLVSTVSHELKTPLSSIRMGLHLLLEEKIGPLNPKQLELLLAAREDSER
                     LLRMINDLLDLARLESGQTRQRFEIVSPGTLIKNAAPDLKPLVEANDARLVTDTPAEL
                     PDVAVDLRQIGHVFSNLVANAARHSMPGEDVVLSAKPIGKSVRFSVLDHGPGIPKEFQ
                     SRIFERFFRIPGTEDSTGIGLGLAIAKEMVVSHGGSIGLQSTSGKGSEFYFDLPAIRK
                     GAKA"
BASE COUNT          391 a          530 c          427 g          407 t
ORIGIN      
        1 agcagatacc ggcacgaagt gcctgggtag aaatcgtttc ttcatgcctt cgctcctttg
       61 cggatcgccg gaagatcaaa atagaactcg ctccccttgc ctgaggtact ttgcagaccg
      121 atgcttcctc cgtgcgaaac gaccatctct ttcgcgatcg ccagtccaag tccaatccct
      181 gtcgagtctt cggtccctgg aatgcggaag aagcgttcaa aaattcgcga ttgaaattct
      241 ttggggatac cgggcccatg atcgagcacc gaaaaccgga cgctctttcc gattggtttt
      301 gctgacaaaa caacatcctc gccgggcatt gagtgccggg cagcattggc aacaagatta
      361 gagaatacat gtccgatctg gcggaggtcc accgcgacgt ccggtagctc tgcaggggta
      421 tcagtcacta accgggcatc atttgcctcc accaagggct tcagatccgg agctgcgttc
      481 ttgatgagcg ttcctgggga aacaatctcg aagcgctgac gtgtctgacc cgattccaag
      541 cgggccaggt ccagcaaatc gttgatcatc cgcaacaacc tctcggagtc ctctcgcgcg
      601 gccagcagta attccagttg tttcgggttg aggggcccaa tcttttcttc caacaaaaga
      661 tgaagcccca tgcgaatgct tgagagtgga gttttcaact catggcttac ggttgagacg
      721 agattggttt ttacctcatc catgagccgg aaccgcgtaa catcctggag gactatagcg
      781 gcaccaaaca gcttacccga ctcatccctc attccgatca ctcgcggcag taagtagacc
      841 tcatgttcat tgacacgaac cgctacggct tcttcgaaac tggtcggcag gtagtccgta
      901 gcacctttga gcaccttttc cgcctcctcc cggatccgga gagggagccc aactttcgag
      961 tcaagccctc tgagcaattc ttcggcggcc ggattggcga aatctatcct ggcgtcgccc
     1021 gagagggcga gaatggcgtc gggaaacgcc gagaaagtga tctccgtcat ttggcgcgcc
     1081 tgtagaattt gatctgccgt gatctgacgg taagcccgca atttcgcaga gagtttattg
     1141 aacgccgccg ccaacgcccc gatctcgtca ttggactgca ccggaacgac ctgatccaga
     1201 ttaccattcc cgagttcttt ggccactgcg gtcaagattc caatcggctt aaatatagct
     1261 cgccgggtgg cgaaataaac cgagactacg atccccaccc caatggccca gacgacgaac
     1321 atggttccag tagaattggt acttaaggtt cgtgcctgcc ggcccacctg gacggtattt
     1381 acatcgttga cagcaacgac ctcttccaat tcattcttga tctcggtaaa cagcggcaac
     1441 aactcggccg tatacatcgt tcgtcgtgcg ccgggctcgg aggtgttcca gaatttcccg
     1501 gccaagtcct ggtaatgctc ttccgcttgt ttgatcctgt ccgctacttc cccttctccc
     1561 ggtaaccaaa cgctcgcccc ttttcctccc aggaatctct cgaaaacagg ggccaagctc
     1621 tcaaacagct ttcgtccctc ctcctctttc ccagccaagg ccaaagacag ggcagaatcc
     1681 atccgtccag cgctttcttt cattgcctca ccgcccaaga cccattgacg gctatcactc
     1741 acgatcacct cgaga
//
LOCUS       DATMKH010000218         1529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3307885, whole genome shotgun sequence.
ACCESSION   DATMKH010000218 DATMKH010000000
VERSION     DATMKH010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3307885"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..250)
                     /locus_tag="VNV63_02690"
     CDS             complement(26..250)
                     /locus_tag="VNV63_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016875794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HXC61560.1"
                     /translation="MDYRSIITIEPGKRSGKPCIRGLRITVYDVLGMLAAGMSHTEIL
                     ADFPYLTEEDIKACLAYAADSDRRLLIVGS"
     gene            complement(1070..1267)
                     /locus_tag="VNV63_02695"
     CDS             complement(1070..1267)
                     /locus_tag="VNV63_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61561.1"
                     /translation="MEPHPKENDQEAVEDSGPKSSPSIGEKLKELLTAKPASYSEDEK
                     PRRAEKEQMSAPQEEFIQEKP"
BASE COUNT          343 a          411 c          406 g          369 t
ORIGIN      
        1 gagagattct cgtcaaagag gagcttcacg atccgacgat gagcaggcgt ctatccgaat
       61 cggcggcgta ggcaaggcaa gctttgatgt cctcttcggt gagataggga aagtcggcaa
      121 ggatttcggt gtggctcatt ccggcggcaa gcattcccaa gacatcatac accgtgattc
      181 gcaggccacg aatacagggt ttaccacttc gtttgcctgg ttcaatcgtg atgatcgagc
      241 gataatccat ggtttcgccg tagtttgaag aatatttaac agccgatccg actcggtgac
      301 aaccggtaag actagaggat tttgtgggct tttctttgga aaagaaagtt cccgtcggct
      361 gcgtcgctcc ccgattctcc gaggaagacc gatgttctcc tataaatcct gacttccatg
      421 gcgcggcctt atgtcaaagg taccgcgctt ccggcagcga aagatcatgg ggcagcccct
      481 ggaggtcaag tggaaccaga tcgacacaga ttcttatatc aagggcacgt ggaaaatccg
      541 aagcatttag ggggacatat tggaaaggcc acgcgcgaaa ttcaaatccc ttgtttcttg
      601 ccgatctgca ctctctcaat ctcctgaaag gtcagccggt ctcctgatcg aatcataaat
      661 cctggtggtc gatggacttt atgtccggca atcctctgcg cgatctcgat cgtgccaccc
      721 gaacttcagc tcttgaactc tgtccatggg cgccctggag gctcctggaa ggggcggact
      781 ggcgccaaca tcagaggttt ctcagcgctg ctctgtgcgg tgggaaatta ccggttccac
      841 agctgaaaag gtccacagag cacaaggcgg gttctttata ccactcttcg tagctgccca
      901 gaggacttga tgaacaagtt cacggcagcg ctaagtcgac ctgaaccctt cctccgcaca
      961 gccggggaag gatggacatc aaccgcagcc ccaccagcaa tcattgattg ctcatcacca
     1021 acgccaatag ctgaccgttt accgtctact tctccttgct tttgctcggt tacggcttct
     1081 cctggatgaa ctcttcctgc ggggcggaca tctgttcctt ttccgccctc cgaggttttt
     1141 cgtcttccga atacgaggct ggcttggcag tcaaaagttc cttcagtttt tctccaatac
     1201 tcggcgagct tttggggccg gagtcttcga cggcttcttg gtcgttctct ttcggatgcg
     1261 gttccatgcg atattaggtt cgcaaacaac gagaactgtg gatctagacg tacaaacgac
     1321 cagtgaataa ggggcagcgg gataagattc aatgcagaga aagccagccc gatcccgaaa
     1381 ttgagcaaat cggtccatcg aagaggagct cgtccaagcc ttaccccgag cgaccccctc
     1441 gaaagttggg cattttgcgg gctcgggtgc ggttggtctc tttggtcgag attcgccgct
     1501 gacggccttg atggtcccga aaacaagcc
//
LOCUS       DATMKH010000219         1477 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4269492, whole genome shotgun sequence.
ACCESSION   DATMKH010000219 DATMKH010000000
VERSION     DATMKH010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1477
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4269492"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /gene="ilvN"
                     /locus_tag="VNV63_02700"
     CDS             <1..441
                     /gene="ilvN"
                     /locus_tag="VNV63_02700"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HXC61562.1"
                     /translation="GFNVESLSVGPAIDPTVSMMTIVTSGDDRVLEQIMKHLNKLIDV
                     IKVVDLTESEYVERETALIKVHTRPEDRAEALRVADIFRAKIVDSTSNTYTIEITGDT
                     KKIQAIINLLRPLGIKEVVRTGRIAIAREEVKVAQVMEKKASGQ"
     gene            468..>1477
                     /gene="ilvC"
                     /locus_tag="VNV63_02705"
     CDS             468..>1477
                     /gene="ilvC"
                     /locus_tag="VNV63_02705"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HXC61563.1"
                     /translation="MKIYYEKDADLKLLKGKTIAIIGYGSQGHAHANNLKDSGLEVVV
                     GLREGNSWAKAEKAGLKVVPTAQAAKMADIAMVLTPDELQADLYKNELGSHLKTGAYL
                     AFAHGFNIHFGQVVPRPDINVFMVAPKGPGHLVRSEFTKGSGVPMLLAIHQDPSKNAR
                     EIGLAYASAIGGCRAGVIETTFREECETDLFGEQVVLCGGITSLITTAYETLIEAGYS
                     PEMAYFECLHEVKLIVDLIYEGGISNMRYSISNTARYGDLTRGPRIINEATRQEMKRI
                     LTDIQGGQFAKEWILENRAGRPVFNALSKRGESHPIEEIGAKLRAMMPWLKKDRLVDK
                     SK"
BASE COUNT          364 a          348 c          459 g          306 t
ORIGIN      
        1 gggtttaatg tcgagagcct ctcggtggga cctgcgattg acccgaccgt ctcgatgatg
       61 acaatcgtga ccagcggaga tgaccgggtc ctggagcaga tcatgaagca cctcaacaag
      121 ttgattgatg tgatcaaggt ggtggatctg accgaaagcg aatatgtgga acgagagacc
      181 gccctgatca aggtacatac ccgaccggaa gatagggccg aggcgttgcg ggtggcggat
      241 atctttcgag ccaagatagt ggactctacg tccaacacct atacgatcga gattaccggt
      301 gatacaaaaa agatccaggc gattatcaac cttctccggc ctctggggat caaggaagtg
      361 gtgcggaccg gacggatcgc gatcgcgcgc gaagaggtga aggtagcgca ggttatggag
      421 aagaaagcca gcgggcagtg atgaagtttt tataaggagt ataaagaatg aagatctatt
      481 atgaaaaaga tgccgattta aaactgctca agggcaagac gattgcaatc atcggttacg
      541 ggagtcaagg tcatgcccat gcgaacaatc tcaaggacag cgggctcgaa gtcgtcgtgg
      601 gcctgaggga aggtaactcc tgggccaagg ccgagaaggc cggtctcaaa gtcgtgccga
      661 ccgctcaggc ggccaagatg gcggacatcg cgatggtcct gactccggat gagcttcagg
      721 ccgatttata caaaaatgaa ctggggtccc acctgaagac cggagcctat cttgccttcg
      781 ctcacggttt caacattcat tttgggcagg tggtcccgcg cccggacatc aatgttttta
      841 tggtggctcc gaaggggccg gggcatcttg tacgctccga gtttacgaaa ggcagcggcg
      901 tgccgatgct gttggctatt catcaggacc cttcgaagaa tgcccgtgaa attgggttgg
      961 cgtatgcgtc ggccatcggt ggatgccgtg ccggcgtgat cgagaccacg tttcgtgaag
     1021 aatgcgagac cgatctcttt ggagaacagg ttgtgctctg cggcgggatc acctcgttga
     1081 tcacgaccgc ctacgagacg ctgattgagg ccgggtattc accggagatg gcctactttg
     1141 agtgcctgca tgaggtgaag ctgatcgttg acctgatcta tgagggcgga atttctaaca
     1201 tgcgctactc gatcagcaat accgcacggt atggggatct cacccgtggg cctcgtatca
     1261 tcaatgaggc cacccgtcag gagatgaaac gtatcctgac ggatatccag ggcggacagt
     1321 ttgcaaagga atggatactg gagaaccgag ccggacggcc ggtcttcaat gccctgtcca
     1381 agcgtggaga gtcccatccg atcgaggaga ttggtgcaaa gctccgggcc atgatgccat
     1441 ggttgaagaa agatcggctg gtggacaaga gtaagaa
//
LOCUS       DATMKH010000220         2012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_423501,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000220 DATMKH010000000
VERSION     DATMKH010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423501"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..960)
                     /locus_tag="VNV63_02710"
     CDS             complement(<1..960)
                     /locus_tag="VNV63_02710"
                     /inference="COORDINATES: protein motif:HMM:NF024646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HXC61564.1"
                     /translation="MKKLKKLISHLSVALLNAPFLRPLPRQSFPDNLAPKLPRRASSR
                     GGHFSLKKVQAEPSTDLSVAIHRASNFVLGMQEPEQGYWADELEADSTLTSEYIMLRH
                     FLGKVNRQKQAKAVRYLLDQQLPNGGWPIYHGGPSEISASIKAYFALKLAGISPDETC
                     LKKARENILAKGGVLKANVFTKITLALFGQFDWRGIPTMPAELIFLPSWFYFNLYAVS
                     YWSRAVIVPLLILFAVKPLCRIPSELGIEELYLEPRSLIKEYFKKDPKPFTWRNFFLA
                     VDRVIKVYDRILPAALRRRAIQKAADWMISHMRGDGGLGAIYPA"
     gene            complement(1002..>2012)
                     /gene="hpnH"
                     /locus_tag="VNV63_02715"
     CDS             complement(1002..>2012)
                     /gene="hpnH"
                     /locus_tag="VNV63_02715"
                     /inference="COORDINATES: protein motif:HMM:TIGR03470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HXC61565.1"
                     /translation="KEDIVRFPIQMTLSLSWYLIKNKLKGRERFPLVMMLEPLHLCNL
                     ACDGCGRIREYEETIQDKMPLERCLKAVDECDAPIVSVCGGEPLIYPHIVDLVNGILD
                     RKKHVMVCTNAIGLEKFLPKFRKTPYFTINVSMDGLAKTHDVTRGRKGLYELDLKAIR
                     AAKAQGFRVVTNTTVYKETDVEEIEGMFEDLTAAGVDGMLVSPGYHYEAVQEDIFLNR
                     HEIHKKFQYISKLADRYRLVNTPLYLDFLTGARQFDCTPWGNPTFNPQGWKGPCYLIT
                     DQHYQTYDELMGQTDWRKYEKREDARCANCMMHCSVEPTVVRETGKNFRDMLTMIKWN
                     LS"
BASE COUNT          463 a          537 c          522 g          490 t
ORIGIN      
        1 cgccgggtag atcgcgccca gtccgccgtc gccccgcata tggctgatca tccagtcggc
       61 ggccttctga atcgcacgtc tccgcaaggc agcgggaaga atgcgatcgt acaccttaat
      121 gactcgatcc accgccagga agaagtttct ccacgtgaac ggcttcgggt cttttttaaa
      181 atattcttta atcaggctcc tgggttccag gtagagctct tcgatgccaa gctcggatgg
      241 aatacggcag agcggtttga ccgcaaaaag gatgagcagt ggaacgatta cagcccgcga
      301 ccagtaggac accgcataga gattaaagta gaaccagctc ggtaaaaaaa tgagctcggc
      361 aggcatcgtt gggatgccac gccaatcgaa ctggccaaag agggccaagg tgattttcgt
      421 gaagacattg gcttttaaga cgccgccctt cgccagtata ttctcccgtg ctttcttaag
      481 gcaggtctca tccggcgaga taccggcaag cttcagtgca aaataggcct taatcgaggc
      541 gctgatctcg ctgggaccgc cgtggtagat gggccatcct ccgttcggga gttgctggtc
      601 cagcagatac cgtaccgcct tggcctgttt ctgccggttc actttgccca gaaaatggcg
      661 cagcatgatg tattcggaag tgagggtgga gtcggcctcc agttcatccg cccaatagcc
      721 ttgctccggc tcctgcattc ccaagacaaa gttgctcgca cgatgaatcg cgacagagag
      781 atccgtggac ggctcggcct ggaccttttt taaggaaaaa tggcctcccc tggaagatgc
      841 ccttcgaggc agcttcgggg cgaggttatc cggaaacgat tgcctgggaa gcggtcttaa
      901 gaagggcgcg tttaaaaggg caacggagag atgcgaaatg agtttcttta atttcttcat
      961 gccgtatatt cacgtttaat tcataagccc ggagtagctt ttcaagataa attccattta
     1021 atcatggtca gcatatctcg aaaattcttt ccggtctcgc gcacgacagt cggctccacg
     1081 ctgcagtgca tcatgcagtt ggcgcaacgg gcgtcctccc gcttctcata ctttctccag
     1141 tccgtctggc ccatcaactc gtcataggtt tggtaatgct gatcagtgat caggtagcaa
     1201 gggcctttcc agccctgagg attgaatgtt ggattgcccc agggtgtgca gtcaaactgt
     1261 cgtgcgccgg ttaagaaatc cagatacaaa ggtgtgttga ccaagcgata ccgatcggcc
     1321 agttttgaga tatactgaaa ttttttgtgg atttcatgcc ggtttaagaa gatatcttcc
     1381 tgtaccgcct cgtaatgata gcccgggctc acaagcattc cgtctacgcc ggctgcggta
     1441 agatcttcaa acatcccctc gatttcttca acgtctgttt ctttgtaaac tgtcgtgttc
     1501 gtcaccacac ggaagccctg ggcctttgcc gcccgaatcg ccttgaggtc gagttcatag
     1561 agccccttgc gcccgcgtgt gacatcatgc gtcttggcca gcccgtccat ggagacatta
     1621 atcgtaaaat agggtgtttt tctaaacttc ggcaagaatt tttcaaggcc gatcgcgttg
     1681 gtgcagacca tgacatgctt cttccggtcc agaataccgt tcaccagatc gacgatatgg
     1741 ggatagatta gcggctcgcc cccgcagacg gagacaatgg gggcatcgca ttcatccacc
     1801 gccttaagac accgctccag gggcatcttg tcctgtatgg tctcttcgta ctcgcgaatc
     1861 cggccgcagc catcacaggc caggttacaa aggtgaagag gctccagcat catcacgagc
     1921 gggaaacgct ctcgtccctt cagcttgttc ttgatcaaat accaggataa agaaagggtc
     1981 atctgtattg gaaaacgcac tatatcctcc tt
//
LOCUS       DATMKH010000221         1196 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1065999, whole genome shotgun sequence.
ACCESSION   DATMKH010000221 DATMKH010000000
VERSION     DATMKH010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1065999"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..981)
                     /locus_tag="VNV63_02720"
     CDS             complement(<1..981)
                     /locus_tag="VNV63_02720"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HXC61566.1"
                     /translation="MTLASSFHAASWGLYPTMRLPERTCSFSVEDGGGKDINNPVRAL
                     MLSFFLGLLAGTSFAQEKVVQTFDGATDLTTPAFQVQDKWEVRWNSPEVISMTILSPD
                     GAVVAGASASSKGSLYLPKGGNFHLQISGSTEKDAAQWHVSVVEIDSTAAGAKETNDV
                     ADYVPPQIISAPVSTNITSTPVAPSTATTSLAPGGSTPPVAGALTEDQAHAVVVIKGD
                     VGEGTGFLIKTADGPAVVTNLHVISANPNVKILTTTGAQIKTLSLKGASDRDLAMFMI
                     QDDHYTYLDLATDIKDTVQTDDEVITPGNSEGGEVVLNTKGKVLGIGPERI"
BASE COUNT          279 a          320 c          322 g          275 t
ORIGIN      
        1 gattctctcc ggaccaattc cgagcacttt tcccttggtg ttaagcacga cctcgccacc
       61 ttcgctgttg cctggagtta tcacttcatc atcggtctgc accgtatcct tgatatcggt
      121 agcgagatca agataggtat aatgatcgtc ttgaatcata aacatggcca ggtcccggtc
      181 ggaagcgccc tttagcgaaa gagttttgat ttgagcaccc gtggtcgtca gaattttaac
      241 gtttggattc gccgagataa cgtggagatt tgtcacgacc gccgggccat ctgcggtttt
      301 aatcaggaag ccggttccct ctcccacgtc gcccttaatc acgacaactg catgagcctg
      361 atcctcggtt aaagctccag ccaccggagg cgtcgaacct ccgggtgcga gcgacgtagt
      421 tgccgttgat ggagcaaccg gtgtggaagt tatattggtt gataccggcg cagaaatgat
      481 ctggggcgga acgtagtcgg cgacgtcatt cgtttctttc gctcccgcgg cggtggagtc
      541 gatttccaca actgagacgt gccattgggc cgcatctttt tcggtgctcc cgcttatttg
      601 aagatggaaa ttccctccct tgggcagata gagagagcct ttcgacgagg cggaagcacc
      661 cgcgacgacg gctccatccg gagagagaat ggtcatgctg atgacctcgg gggaattcca
      721 tcgcacctcc catttgtcct gcacttggaa tgcaggcgtg gtcaggtcgg ttgcgccatc
      781 aaaagtttgc acgacctttt cctgtgcgaa ggaggtgcca gcgagcaatc ccagaaagaa
      841 ggatagcatc aaggcacgca cgggattatt tatatccttt cctcccccgt cttcaacgga
      901 aaaggaacag gtgcgctcag gcaacctcat cgtggggtaa agaccccatg acgcggcgtg
      961 gaacgagctg gctaaagtca cggcatgaca acacgaaata aagcaaaaaa cttttccaac
     1021 cgcgtcaaaa ccaggaacct gaccttcgga gatttggtcg cctcgactta tgacgaactc
     1081 ggggaagaag gggcccgtcg cattcttcaa ctcgccatgg aatcgcatct gattacgttc
     1141 tcacggtcga agtgcatgag ttaacgaacc aaggaggaaa gcctctcgca agctcg
//
LOCUS       DATMKH010000222         1461 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1066223, whole genome shotgun sequence.
ACCESSION   DATMKH010000222 DATMKH010000000
VERSION     DATMKH010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1461
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1066223"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="VNV63_02725"
     CDS             <1..578
                     /locus_tag="VNV63_02725"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HXC61567.1"
                     /translation="LAAIALLLSGHIEWTDIIWEPSALFISFGWMTLIAFSEELVFRG
                     YILGNLMEWVPNKWIALGISSVLFTAYHAITPGIHTLAFVNLFLAGMLLGINYIFTRN
                     LWFSFLLHLGWNFFEGPILGFKVSGTTFPSLFQAEPNGDLFITGGDYGLGGSMPMTLF
                     LFVAILVLAWAFERKYQRVTITTEPESAPEP"
     gene            complement(604..1059)
                     /locus_tag="VNV63_02730"
     CDS             complement(604..1059)
                     /locus_tag="VNV63_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009281857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HXC61568.1"
                     /translation="MAQEVSKGDTIKIHYRRRLTDGTTFDSSEGRPPLEFEVGSGMVI
                     KGFDEGVMGMSVGQKKTIEIPAEFAYGPRDPEAIIEFPIDQFPPQMKPEVGMQLVLTD
                     NSGHQIPVLVVDVKLEVVILDANHQLAGQDLIFDLELVEIKGASRIIIP"
     gene            1166..>1461
                     /locus_tag="VNV63_02735"
     CDS             1166..>1461
                     /locus_tag="VNV63_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61569.1"
                     /translation="MRAANLFLIFFLLSGTADPQSTASGGAHPLPQPGSAEQPAQAPS
                     AVLHILTGADQLDQYLPLLEGQSVAVFANATSVIGHTHLVDTLVKLGIHIQKIFS"
BASE COUNT          312 a          452 c          315 g          382 t
ORIGIN      
        1 gcctcgccgc catcgccctt ctgctgagcg gtcacatcga atggaccgat attatctggg
       61 aaccgtctgc attatttatc tccttcggct ggatgacact cattgccttt agtgaagaac
      121 tcgtcttcag agggtatatt cttgggaacc tcatggagtg ggtccccaac aaatggattg
      181 ccctcggcat ctcatccgta ctcttcacag cgtatcacgc tatcactccc gggatccata
      241 ccctcgcctt cgtcaatctg ttccttgccg gaatgctgct ggggattaat tacatcttca
      301 cccggaatct ctggttctct tttctcctcc atctcggctg gaattttttt gaaggcccta
      361 tactcgggtt caaggtcagc ggaacgacct tcccatcgct attccaggca gaacccaatg
      421 gcgatctttt tatcaccggc ggcgattatg gactgggagg atcgatgccg atgaccctct
      481 tccttttcgt tgctattctt gtgctggcct gggcctttga acggaaatat cagcgcgtga
      541 ccattacaac tgaacccgaa tctgcccccg aaccttaatc cccaccttcc aatccgcgcc
      601 gtcctacggt ataataatgc gcgatgcccc cttgatctcg acgagttcga gatcgaagat
      661 cagatcctga cccgccagct gatgattggc gtccaggata accacctcca gtttaacatc
      721 cacaaccagt acgggtatct gatgtcccga attatccgtc aacaccagct gcatcccgac
      781 ttcgggcttc atttgcggcg ggaactgatc gattggaaat tcgattatcg cctcaggatc
      841 tctcggccca tatgcaaatt ccgcagggat ctcgatcgtc ttcttttgcc cgaccgacat
      901 ccccatgacg ccctcatcga accccttgat caccatcccg ctgccgactt cgaactccag
      961 cggcggacgc ccctctgaag aatcaaacgt cgtgccgtcg gtcagccttc gccgataatg
     1021 gattttgatg gtatcgcctt ttgaaacttc ctgtgccatg tctgcttata tttatagttg
     1081 ctcccaatat cggggtgcga tgcaaggtag gattttataa ttaaattgtg cctgtttcca
     1141 accatccgcc aagcaattat aaaccatgcg agctgctaat ttatttctga tcttcttcct
     1201 tttgtccggc acggcggacc ctcagtcaac cgcatccgga ggcgcccacc cgctccctca
     1261 accggggtcg gcagaacagc ccgcacaggc gccctcagca gttctccaca tcctgaccgg
     1321 cgctgatcag ctcgaccaat acctgccgct gctggaagga caatccgttg ctgtattcgc
     1381 aaacgccacc tcggtcatcg gccatacgca tctggtagat acactcgtca aactaggcat
     1441 ccatatccaa aaaatatttt c
//
LOCUS       DATMKH010000223         1581 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2025183, whole genome shotgun sequence.
ACCESSION   DATMKH010000223 DATMKH010000000
VERSION     DATMKH010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2025183"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..772
                     /locus_tag="VNV63_02740"
     CDS             164..772
                     /locus_tag="VNV63_02740"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXC61570.1"
                     /translation="MTSPVPEQNYYDLLEVHVSASKEEIQQAYDLACKTFGEQSLASY
                     SLFATEERKALLKRIEEAYRVLVDEHSRREYDQSLPKKPASREAAPPPPPLPAAKIPS
                     RKELEDLLVANQEEGLTGQILEHIRKALGVPLQEIANKTKISITYLQFIEENKYKGLP
                     VEVYLRSYLVQYARVLGFDPKKIADGYLKSWRRWQESNQEPP"
     gene            complement(726..>1581)
                     /locus_tag="VNV63_02745"
     CDS             complement(726..>1581)
                     /locus_tag="VNV63_02745"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXC61571.1"
                     /translation="RPHSPYCYRCHLGLAFPSCQISCIEEVENILRDHRDELAALIVE
                     PMLQAAGGILVFPHGYLRRLREACTRYRVLLICDEVATGFGRTGKMFACEHEGVTPDL
                     MAVAKGLTGGYLPLGATMTTQEVYDAFLGKYEEFKTFFHGHSYTGNQLGCAAALASLK
                     LFEQKKILKKLPAKIRYLAKLLAPFKSMAHVGDVRQMGMIAGIELVKLKSHMTPYPIE
                     ERWGMKVTSEARERGMLLRPLGNVLVLMPPLSVTQKELKRMVEILYDSIVAVTEAPGS
                     IPATASNS"
BASE COUNT          388 a          407 c          424 g          362 t
ORIGIN      
        1 acatcggcta tatagaatat gacgactgtg tgtggcaggc gacgaagaag aagagaccgc
       61 tgctcatcga gtatccctat tcgggttccg cgaggtgtat cgagcgagtc acgcaccatc
      121 ttttgaagca tgagcagctg acctttgatt ttctcatcca tccatgactt ctccggtacc
      181 tgaacaaaat tattacgacc tcctcgaggt gcacgtctcg gccagcaagg aagagatcca
      241 gcaggcatac gacctggcgt gcaaaacttt tggcgagcaa tctcttgcct cctattcttt
      301 gtttgcaaca gaggaacgta aggcattgct taaacgaatc gaagaggcgt atcgggtctt
      361 ggttgatgag catagccgcc gggagtacga tcagtccttg cctaaaaagc cggcatcccg
      421 ggaggctgct ccgcccccgc ctcccttgcc agcggccaag attccaagca ggaaagaact
      481 ggaagatctc ctggtcgcga atcaagaaga gggactgacc ggccagatcc tggagcatat
      541 ccgaaaggcg ctcggcgtcc ctctgcaaga aatcgcaaac aaaacaaaga tcagtatcac
      601 ctacttacag tttattgagg agaataaata taagggttta ccggttgaag tgtacctgcg
      661 gagttatctc gttcagtatg cccgggtgct cgggtttgat cccaaaaaaa ttgcggatgg
      721 ttatctcaag agttggaggc ggtggcagga atcgaaccag gagcctccgt aactgctacg
      781 attgaatcat acagaatctc gaccatgcgt tttaattctt tttgggtgac cgagagcggc
      841 ggcattagga ccaggacatt tccaagcggg cggagaagca ttccgcgttc cctcgcttcc
      901 gaagtaacct tcattcccca acgttcttca atcggataag gcgtcatgtg gctttttagc
      961 ttgacgagtt cgattcccgc gatcattccc atctgtcgga catcacccac atgcgccatg
     1021 gatttgaaag gcgcaaggag tttggccaga taccggatct tggccggaag ttttttgagg
     1081 atcttttttt gctcgaataa cttgaggctg gctaaagcag ccgcacatcc gagctggttt
     1141 ccggtgtagc tgtgaccgtg aaagaatgtt ttgaactctt catatttccc aagaaaagca
     1201 tcgtatacct cttgggtggt catcgtggcg ccgaggggga ggtagccgcc ggtgaggcct
     1261 ttagccaccg ccatgagatc cggcgtgacc ccttcatgct cgcaggcgaa cattttgccg
     1321 gtccggccaa aacctgttgc cacctcatcg cagatcaaca ggacacgata acgcgtacag
     1381 gcctccctga gtcgccggag atagccgtgg ggaaaaacca gtatgccacc cgccgcctgg
     1441 agcatcggct ctacaatcag ggcagcgagt tcgtcgcgat ggtcccttag aatattttcg
     1501 acctcttcta tgcaggagat ctgacacgaa ggaaaagcca ggcccagatg gcatcgatag
     1561 cagtaggggg aatgaggccg a
//
LOCUS       DATMKH010000224         1078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_742924,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000224 DATMKH010000000
VERSION     DATMKH010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_742924"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..207
                     /locus_tag="VNV63_02750"
     tRNA            131..207
                     /locus_tag="VNV63_02750"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:165..167,aa:Pro,seq:tgg)
     gene            complement(277..>1078)
                     /locus_tag="VNV63_02755"
     CDS             complement(277..>1078)
                     /locus_tag="VNV63_02755"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HXC61572.1"
                     /translation="MIEDFKAGRAKELRKGSKTQLVSPATVNRELGTLKKILNMAVDD
                     ELITINPARKVKPFPEEIPDVHILSFKEELDYLAFASEPLKSVAIIMLGQGMRPDEVF
                     RMTYSNLDFFNRTILIVRGKTKNAKRTLSMCQEVFELLKKRRQNQPNDYWVFPSSRKP
                     GTHLGSVRKAHDRVCDDLGLRGAMDLYNLRHTFATRSILSGTDVTILQKILGHSEIGM
                     TMRYVKIAEHYKVEAVDRLEKYRAVKMQEVVVKMREEQQEAEKMGMVQ"
BASE COUNT          217 a          289 c          283 g          289 t
ORIGIN      
        1 gcctgattac gaaaggccaa gggccgccca tccagaaaga gacctaggaa tttattcatg
       61 gcggcgatat tggtcatatg aagcaaccgt tctgttggga agtgctttat aatcttttcg
      121 cacttcatgt cggggcgtag cgcagcctgg tagcgcgcct gccttgggcg caggaggcct
      181 ccggttcaaa tccggacgcc ccgaccaatc ttttctgcga cttgcagaca tctctaaaaa
      241 gtccttcccc tcactatggg ggaaattcag gggaccctac tgcaccatcc ccatcttttc
      301 cgcttcttgc tgttcttcgc gcattttaac cactacttcc tgcatcttca ctgctcgata
      361 cttctcgagc ctatctactg cttccacttt gtaatgctcc gcaattttca cgtaacgcat
      421 cgtcatcccg atttctgagt gcccgaggat tttctgcaaa atcgtgacgt cggttccgga
      481 caggatggat cgggtggcaa atgtgtgacg caggttgtag aggtccatgg ccccgcgcaa
      541 tcccaagtcg tcgcagacgc gatcatgggc tttgcggacg gaaccaagat gggtaccggg
      601 tttccgagaa ctcggaaaaa cccagtagtc gttgggttgg ttctgccggc gtttcttcag
      661 gagttcgaag acttcctgac acatcgacag agtacgtttg gcgtttttgg ttttcccgcg
      721 cacgattagg atcgttcggt tgaagaagtc caggttcgaa taggtcattc gaaagacttc
      781 atccggccgc attccttggc cgagcatgat tatggcgacg ctcttgaggg gctcggaggc
      841 aaatgccaga tagtcgagct cctctttgaa cgacaggatg tggacgtctg gaatttcctc
      901 cgggaaaggt ttcactttcc tggcggggtt gatggtgatc agctcgtcat cgacggccat
      961 gttcaggatc tttttcagag taccaagttc ccgattgacc gtagccggtg acacgagctg
     1021 cgttttgctt cccttcctca gttccttggc tcgaccggcc ttgaagtcct caatcatg
//
LOCUS       DATMKH010000225         2199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2668569, whole genome shotgun sequence.
ACCESSION   DATMKH010000225 DATMKH010000000
VERSION     DATMKH010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2668569"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /gene="gpmA"
                     /locus_tag="VNV63_02760"
     CDS             <1..621
                     /gene="gpmA"
                     /locus_tag="VNV63_02760"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HXC61573.1"
                     /translation="KREEYTFDVAFTSVLKRAIKTLWIVFEEMDLMWIPVHHSWRLNE
                     RHYGDLQGLDKAETARRFGKEQVHIWRRSYDIRPPALAPDDKRSAGQDPRYAGLQPEE
                     IPLTESLKDTIGRFLPYWHEVIVPAVRKGERVLIVAHGNSLRGLVKTLDGIPDDQIAE
                     LNIPTGIPLVYELENDLKPIRHFYLGEPDSVEQAIQKTSRPFRKET"
     gene            787..1617
                     /locus_tag="VNV63_02765"
     CDS             787..1617
                     /locus_tag="VNV63_02765"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HXC61574.1"
                     /translation="MTTIKDIMTDKLLTISKDATLREAAKIMKTKKVGSLLVKNDKEF
                     PGILTETDIVQKSIGLDRPPEFTKVEEAMSSPLLTIKADSPILEATDLMTERCVRHLA
                     VTEKKAVIGIISIRDVLQSKELPWISIRRLMSMAIFALIRGSTISDAAKLMKHNKIGS
                     LLVTGRRGRPRAEPFFIGNRKEITGILTETDIVRKVVAGGLDPLTTPIEKVMSRALTV
                     INTWKRAAKACDMMAQKGIRHLPVTEDQKIIGMVSIRDLINPLYYSHGAPMDEQLYAR
                     "
     gene            1595..>2199
                     /locus_tag="VNV63_02770"
     CDS             1595..>2199
                     /locus_tag="VNV63_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HXC61575.1"
                     /translation="MSNYTHDERSKAQVITMELPLQITVRNVSVSEAVMDDIRNKAVK
                     LGHFSDNIIGCRVTVDGPHRHHHQGVLYNILIALAVRGAELVVKRQPNEDIYVAIRDA
                     FDAVHQKLENYERRLRGEVKTHQTASHARVIKLFPKSGYGFLEAPEGHEIYFHENSVL
                     NGAFNRMKIGTEVRFVEEPGEKGPQASTVVIVRKARARQAR"
BASE COUNT          583 a          561 c          609 g          446 t
ORIGIN      
        1 aagagagaag aatacacgtt tgacgtcgcg tttacatccg ttctcaagcg ggcgatcaag
       61 acgctctgga tcgtgttcga agaaatggac ctaatgtgga tcccggtgca tcatagctgg
      121 cgcctcaatg aacggcacta cggcgatctt cagggcctgg acaaggcgga gacggctcgc
      181 cgttttggga aggaacaggt tcatatctgg cggcgcagct atgatatccg gccccccgcg
      241 cttgcgcccg atgacaaacg ctctgccggt caggatcccc gctacgccgg gcttcagccg
      301 gaagaaatcc cgctgacgga atcactgaaa gatacgatcg ggcgtttttt gccttattgg
      361 cacgaggtga tcgtgccggc ggttcgaaag ggagaacgcg tcctgatcgt cgcgcatggc
      421 aacagcctcc gcgggcttgt aaaaactctt gatgggatcc ctgacgatca gatcgcggaa
      481 ctgaacatcc ctacgggtat cccgctcgtt tatgagctgg agaacgattt aaaaccaata
      541 cggcacttct atctgggaga accggattcg gtcgagcagg cgattcaaaa gacctcaaga
      601 ccttttcgta aagaaacctg aggagggcag ggagcaaaaa gcttgcggcg gtgctcctca
      661 cgcttatcct tcgcatgatg tgatagatca tacagacctg atgaccgatg tcatagaatt
      721 aagaacgggg ttgagttata tttaacctgc cgccaaagac aatctttaaa gaagggggga
      781 gagaccatga cgactataaa ggacatcatg acggacaaac tcttaactat ctctaaagat
      841 gccacgcttc gagaggctgc aaagatcatg aaaaccaaaa aagtgggaag ccttctggta
      901 aagaatgaca aagaattccc gggcattctc accgaaaccg acatcgttca aaaatccatt
      961 gggctcgatc ggccgcccga gttcacaaaa gtggaagagg ccatgagcag tccgctcttg
     1021 accattaagg cagattcccc gattctggaa gctaccgatc tcatgacgga acgctgtgtg
     1081 cggcacctgg cggtaaccga gaaaaaagcg gtcatcggga tcatctccat ccgggacgtc
     1141 ctgcaaagca aagagctccc ctggatatcc atccgaagac tcatgtctat ggcgatcttc
     1201 gccttgatca gagggtcaac aatcagtgac gccgctaaac tgatgaagca caataaaatc
     1261 ggaagccttc tggttaccgg ccgaagaggg cgcccaaggg cggaaccgtt ctttatcggt
     1321 aacaggaaag agattaccgg aatcctcacc gagaccgata tcgtccgcaa ggttgtggcg
     1381 ggaggtctgg accccctgac aaccccgatt gagaaggtca tgagccgggc actgaccgtg
     1441 ataaacacct ggaaacgtgc ggccaaggcc tgcgatatga tggcacagaa ggggatcaga
     1501 catctcccgg tgacagagga tcagaagatt atcggaatgg tatctatacg ggacctcatc
     1561 aatcccctct attactcgca tggggctccc atggatgagc aactatacgc acgatgagcg
     1621 gtctaaggcc caggtaatta cgatggagct gccacttcaa atcacggtac gtaatgtttc
     1681 cgtttccgaa gcagtcatgg atgatatccg caataaggcg gtcaagctgg gtcatttctc
     1741 agataacatc atcggctgcc gtgtcacggt ggatggtccg cacagacatc accaccaggg
     1801 tgtcctctac aacatcctaa ttgctctggc tgttcgcgga gccgaactgg tggtgaaacg
     1861 ccagccgaac gaagatattt acgtggcgat ccgggatgcg tttgacgccg ttcatcaaaa
     1921 gctggagaac tacgaacggc ggttgcgcgg tgaggtaaag actcatcaga ctgcgtctca
     1981 tgcccgcgta atcaagctgt tcccgaaaag cggctacggc tttcttgaag cgcctgaggg
     2041 gcatgaaatc tattttcacg aaaacagcgt gctgaacgga gccttcaatc gaatgaagat
     2101 tgggaccgag gtgcgtttcg tcgaagaacc gggagagaag ggcccgcagg ccagcacggt
     2161 cgtgatcgtt agaaaagctc gcgcccggca ggctcggaa
//
LOCUS       DATMKH010000226         1328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2988461, whole genome shotgun sequence.
ACCESSION   DATMKH010000226 DATMKH010000000
VERSION     DATMKH010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2988461"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..576)
                     /locus_tag="VNV63_02775"
     CDS             complement(139..576)
                     /locus_tag="VNV63_02775"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HXC61576.1"
                     /translation="MPNKLLKVVRGKQSLSVPTPAEMRLLQVLWDREEGTVDEVVNAH
                     PEKERPNYKTTQTLLRIMEQKGFITHESRGRVFVFRPVVSRKTIDSLSVQALVSRNFR
                     GSAAGLLINLLETSSIKKKELDELEAYLREYRKKYEIREHDRG"
     gene            832..>1328
                     /locus_tag="VNV63_02780"
     CDS             832..>1328
                     /locus_tag="VNV63_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61577.1"
                     /translation="MQARLLYPIYVENRIAVPVGTVLRGTVVQLISDRSHRIHSRLRG
                     DFTPFHIPVVRFDQLVLPDGTLQSLVSENARDGAPLLRLSPPPGKKKGSFISRQLDEE
                     KHALKSDAAAITAPGRGDRFVQFLYTQLPYHPERIDSGTSWTVELAQPLELRSDPPGA
                     HPVVS"
BASE COUNT          295 a          392 c          317 g          324 t
ORIGIN      
        1 cgaaaatctc cggttgggtg aatcaaccag ccggctgaag attacgcaca ctccaaaagc
       61 aagcgttgtc ttcaacaaaa agcccgcaag tgcagccacg acacgaagca aaaggtctgg
      121 gttcacggcg cccccgcatc atccgcgatc gtgttcgcgg atctcgtact ttttacgata
      181 ctctcggaga tacgcttcta gttcatccag ctccttcttt ttgatggaac tggtctccag
      241 caaattaata agcagcccgg ccgctgaccc ccggaagttc cgggacacca gcgcctgtac
      301 agataggctg tctatggtct ttcgcgaaac gacgggcctg aagacgaaaa cgcggcctcg
      361 gctctcatgc gtgataaagc ctttttgctc catgatacgg agcaaagtct gtgtagtctt
      421 gtagttgggg cgttcttttt cggggtgagc gttcactacc tcgtcgacgg tgccctcttc
      481 ccgatcccat aacacctgca gaagacgcat ctcagcaggg gtcgggacgg aaagcgattg
      541 cttcccccgc actaccttta acagcttgtt tggcatacgt gcctctcaag agggatctta
      601 acgatttaag attatccttg attttcaaat tcccgaggaa taaagatttc tatcttaaat
      661 ccaatgagac gagctggata ctttaaagca gctcgttttt tcgtctgcgt atctttccta
      721 gccttactcc cggctctttt gcatgcgcaa acggcatcga tccagatccc ttcgacaact
      781 cccttttcgg ttgaattgct ccaacatgtt cctatgaagg ccggggaatc gatgcaagcc
      841 cggctgcttt acccaatcta cgtcgagaac cggattgctg taccggtggg cactgtgctt
      901 cgcggcaccg tagtccagct aatttcagac cgaagccaca ggatccactc ccgcctgcga
      961 ggcgatttca cgccattcca tataccggtt gttcgcttcg atcagttggt cctccccgac
     1021 ggaactcttc aatcccttgt cagtgagaat gctagagacg gagcgccgct tctgcgactg
     1081 tctcccccac cgggcaagaa aaagggatcc ttcatttccc gccagctcga tgaagagaaa
     1141 cacgcgttga aatctgacgc cgcagccatc accgcaccgg gtcgaggcga ccgcttcgtt
     1201 cagtttcttt atacccagct cccgtatcac ccggagagga tcgatagcgg cacctcatgg
     1261 acggttgaat tggcccagcc tctcgagctt agatctgatc cccctggcgc ccatccagtg
     1321 gtttcaca
//
LOCUS       DATMKH010000227         1161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_425024,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000227 DATMKH010000000
VERSION     DATMKH010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425024"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="VNV63_02785"
     CDS             complement(<1..479)
                     /locus_tag="VNV63_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012738892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HXC61578.1"
                     /translation="MAFTDEQIERYSRHILLPEIGGKGQQKIREAKVFIVGAGGLGSP
                     IALYLAAAGVGTLGIIDGDAVDLSNLHRQVIHHTSDLDKPKVYSAKEKIEALNPDVRV
                     ITYLERLVAKNALDILRNYDLIIDGTDNFPAKFLINDAAILTGKPLVHGGILRFDGQA
                     "
     gene            complement(494..>1161)
                     /locus_tag="VNV63_02790"
     CDS             complement(494..>1161)
                     /locus_tag="VNV63_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009371443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HXC61579.1"
                     /translation="GYKLILVMPESMSMERASLLSSYGAQLVLTPAWEGMKGAIREAN
                     SILEQNPSYFMPDQFSNPANPAIHRRTTGPEIWEAMEGKLDAFVAGVGTGGTITGVGE
                     VLKEHNPKTLIVAVEPSASAVLSGGEPGPHKIQGIGAGFVPKVLNRGIIDRIITVSDE
                     DAYRTAKLLAKKEGLLVGISSGANAYAAIQIAEELSPEARVVTLLPDTGERYISIEKY
                     FNI"
BASE COUNT          250 a          348 c          293 g          270 t
ORIGIN      
        1 gcctggccgt cgaaccgaag gatgccgccg tgcaccaggg gcttgccggt caggatcgcc
       61 gcatcgttga tgagaaattt ggcagggaaa ttgtccgtac cgtcaatgat caggtcgtaa
      121 ttccgaagga tgtccagggc gttcttggcc accagacgtt ccagataagt gatcacccgg
      181 acatccgggt taagcgcctc gatcttttct ttggccgaat agaccttggg tttgtccaag
      241 tccgaggtgt gatggatgac ctgccgatga agattggata gatcaaccgc gtccccatca
      301 atgattccca gagtgcccac accggcggca gcgagataca acgcaatggg tgagccgagc
      361 ccgccagccc ccacgataaa gacctttgct tcacggatct tctgctgtcc ttttccgccg
      421 atctccggca atagaatatg ccgactgtac cgttcgatct gttcatccgt aaatgccatt
      481 gttctgccgt cagtcaaata ttaaaatatt tttcaatgct aatatagcgt tcccccgtat
      541 ccggaaggag cgtgaccacg cgggcctccg gactcaactc ctcggctatc tgaatcgccg
      601 cataggcatt cgccccggag gagatgccga ccaaaaggcc ttctttcttt gccaggagct
      661 tggcggtgcg ataggcatcc tcatcgctta ccgtgataat cctatcaata atccctcgat
      721 tcaagacctt tggaacaaac ccggcaccga ttccttggat cttgtgaggg ccaggctctc
      781 cacccgataa cacggcggaa gccgaaggct ccactgccac aatcagggtc ttcgggttat
      841 gctctttgag cacttcaccc acgccggtga tcgtcccacc ggttccgact cctgccacaa
      901 aagcgtccag ttttccttcc atcgcctccc agatctcagg gccggtggtc cgccgatgaa
      961 tggccgggtt ggccgggttc gagaattggt ccggcataaa ataggacggg ttctgctcta
     1021 aaatactatt ggcctctcga atcgctccct tcattccttc ccaggctggc gtcagaacca
     1081 gctgtgcgcc ataagaggac agaaggcttg cccgctccat gctcatgctc tccggcatga
     1141 ccagaatcaa tttgtacccg c
//
LOCUS       DATMKH010000228         1206 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1702740, whole genome shotgun sequence.
ACCESSION   DATMKH010000228 DATMKH010000000
VERSION     DATMKH010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1702740"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1206
                     /locus_tag="VNV63_02795"
     CDS             <1..>1206
                     /locus_tag="VNV63_02795"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXC61580.1"
                     /translation="EVSTESFLRALHPDDWDRIREGMSAAIRERRPYRADYRIILPDG
                     TERNLHSQAEIVYDENTKRPSKMVGIVQDITDRKRSEQELIVAKEAAEAGNRAKSEFL
                     ANMSHEIRTPLNGVMGMTDLALETDLTPEQREYLETVKLSADSLLTVINDILDFSKIE
                     AGKTDLELDDFNLRDSVEATLKTLALQADEKGVELLCEIAPSVPDVVRGDSNRLRQVL
                     VNLIGNAIKFTDHGEVELNVQVEVEDGHNRIIHFTVSDTGIGIPPEKQKLIFQPFAQA
                     DNSTTRKYGGTGLGLTISKRLVELMGGNLWVESELGRGTQFHFTARLKTSEKPIEVGT
                     IAPPEILQGVKVLIVDDNRTNRRILEGMLKRWDMKSTSVEDGKKALMLLSSAQNAGEP
                     YALVLTDMHM"
BASE COUNT          337 a          289 c          341 g          239 t
ORIGIN      
        1 gaagtatcga cagaaagctt cctacgtgcc ctccatcctg atgactggga ccgtatacgc
       61 gaaggtatgt cagcagcgat ccgcgagaga agaccgtata gggccgacta ccgaatcatc
      121 ttgcccgacg gtaccgaacg caacctccac tcgcaggccg aaatcgtcta cgacgagaac
      181 accaagagac cgtcgaagat ggtcgggatc gttcaggata tcaccgaccg gaaacggtct
      241 gagcaggaat tgatcgtggc caaggaagcg gcggaagcgg gcaaccgggc gaaaagcgag
      301 tttctagcga acatgagcca cgaaatacgc accccgctga atggggtcat ggggatgacc
      361 gatctagcat tggaaaccga cctgacgccg gaacaacggg aatatttgga gacggtgaaa
      421 ctctcggcgg actctctact caccgtcatc aatgacattc tcgacttctc caagattgag
      481 gcgggaaaaa cagatttgga gttggacgac ttcaatttgc gggatagcgt ggaagcgacg
      541 ctaaaaaccc tagcgctgca ggcggacgaa aaaggggtag agcttctgtg cgagatagct
      601 ccgtcagttc cggatgttgt ccggggagat tccaaccggc tgcgccaagt cctggttaac
      661 cttatcggaa atgccattaa gtttacagat catggtgagg tggaattaaa cgttcaagtc
      721 gaggttgagg acggtcacaa ccgaatcata cattttaccg tatcggacac gggtattggc
      781 attcctcccg agaagcaaaa attaatcttt caacctttcg cccaggcgga taactcgacg
      841 acaaggaaat atggcggaac cggccttgga ttgaccatct cgaaacgcct ggtcgaattg
      901 atgggaggaa acctgtgggt agaaagcgaa ttagggcgag gaacccagtt tcacttcacg
      961 gcgcggctta agacctcaga gaaaccgatt gaagttggaa cgattgctcc acctgagatt
     1021 ttacagggtg tgaaggtcct aatcgtagac gataaccgta ccaatcggcg gatactggag
     1081 ggaatgctga agcgctggga tatgaaatcg acctctgtag aagatggaaa aaaggcacta
     1141 atgctcctgt cctcagccca aaatgcgggc gaaccgtatg cattggtctt gacggacatg
     1201 cacatg
//
LOCUS       DATMKH010000229         1035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3310762, whole genome shotgun sequence.
ACCESSION   DATMKH010000229 DATMKH010000000
VERSION     DATMKH010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3310762"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..353)
                     /locus_tag="VNV63_02800"
     CDS             complement(150..353)
                     /locus_tag="VNV63_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61581.1"
                     /translation="MSEISTASATPVVVIGFDMPFWPLVRFLIKIAIASIPAGIVIAF
                     IYMVLGWIYGAILLATHHTNLLH"
     gene            complement(668..898)
                     /locus_tag="VNV63_02805"
     CDS             complement(668..898)
                     /locus_tag="VNV63_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61582.1"
                     /translation="MSFVDDLMGMLAEGEIRCKEIRRRLAEIQPVPQERSDPNGEDFF
                     ELKQELDKESTRVGLIEQKLRNVGQWPEPKPV"
BASE COUNT          234 a          281 c          267 g          253 t
ORIGIN      
        1 atatccaaca tccacactct caagaaagag ggattaacgc atttttgcta atactgtggt
       61 ccggtgggga tcagttatgt gcggccattc ttcggcggaa tggccgcgtc gggttcagtt
      121 tcctggtcta caccattagc aaaaacgctc tagtgcaata agttggtgtg atgcgtggcc
      181 agaaggatcg ccccatagat ccagcccagc accatgtaga tgaaggcgat cactatgccg
      241 gcgggaatag aagcaatggc aattttgatc aggaacctga ccaacggcca aaaaggcata
      301 tcgaagccaa tgaccacaac gggcgtagcg gaagcggtgg aaatctctga catgaaaaca
      361 cccttctttc gtgaatgtgt tcctggatct cggatcgttg gcccggctgg gaaagcgttg
      421 tacctgagag attgaagcgc tgacagatgg caagtcgtga tagcgtggga catggaaccg
      481 accgactcca tggtcttgct tttcaagttc gtcgtcgtgg tagcgctatt gctatggttc
      541 tggcatcact tccggggagg tcgtcctccc aatccgatgc atccttctcc agcggatgat
      601 gcccttctcc ttcggaagcg acggagtagg acgcaaggtt gaaaacgatc aaattgaact
      661 aaccccttta gaccggcttt ggctctggcc attgcccaac gtttctcagc ttctgttcga
      721 tcaacccgac tcttgtcgat tccttgtcca gttcttgctt cagctcaaag aagtcctcgc
      781 cgtttgggtc ggaccgttct tgtgggactg gttgaatctc ggcgaggcga cgccggattt
      841 ctttgcagcg gatttcccct tctgcgagca ttcccattag gtcatctaca aagctcatcg
      901 catgctcctc ctcgaaagag tcttgcccac gattatccac ccgaaagggc cgatcggcat
      961 gacgaccagc tgcgccacca ttatttcttg cggcgccccc cactctagac aagccagcgt
     1021 tctagaccct agaag
//
LOCUS       DATMKH010000230         1059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1068305, whole genome shotgun sequence.
ACCESSION   DATMKH010000230 DATMKH010000000
VERSION     DATMKH010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1068305"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..762
                     /locus_tag="VNV63_02810"
     CDS             <1..762
                     /locus_tag="VNV63_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HXC61583.1"
                     /translation="DQTQVTWYGQSAFKIVTPKGHVLFVDPWLENPMNKNGKVDVASI
                     DKADLILVSHGHFDHVGAAAEIARKTKARLVATFDLGANLATYGGFPKEQMGYDSLGN
                     FGGTLSFFEDEVKITFVPAVHSSSVNRKDLGLGPEEENHWAGSPSGFLIRIKSGPTIY
                     HTGDTDLFGDMAQLRKYGNIDLMLACIGDHFTMGPKGAAEAVKLVSPERVVPMHYGTF
                     LPMMTGTPEQFKAELRALGLEKKFVPMVVGQAEHF"
     gene            837..>1059
                     /locus_tag="VNV63_02815"
     CDS             837..>1059
                     /locus_tag="VNV63_02815"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HXC61584.1"
                     /translation="MKPGPLVLTRINETFYQATAALVVEVLTGVGHSVTVVDGSHTSA
                     YEAIKEGTADLCVGFWLPTGHEKAWSQVKD"
BASE COUNT          252 a          249 c          296 g          262 t
ORIGIN      
        1 gaccagactc aggtgacctg gtatgggcag tccgcgttca agattgtgac ccccaaaggg
       61 catgttctat tcgtagatcc atggctcgaa aatcccatga ataaaaatgg aaaagtggac
      121 gtggcttcaa ttgataaggc cgacttgatt ctggtatccc atggccattt tgaccacgta
      181 ggagcggctg ccgagattgc caggaagacc aaggcgcgcc ttgtagctac gtttgatctc
      241 ggagcaaatc tcgccaccta tggtggcttt ccaaaagagc agatgggtta tgactcgtta
      301 ggcaatttcg gcggcacttt gtcgttcttt gaggacgaag tgaagatcac ctttgtgcct
      361 gccgtgcaca gttcgtcagt caataggaag gatctcggat tggggccaga ggaggaaaat
      421 cactgggcag gttctccttc gggctttctc attaggatca agtctggtcc cacgatctat
      481 catacggggg acaccgatct tttcggagac atggctcaac tgagaaagta cggaaacatc
      541 gatctgatgc tcgcctgcat tggcgatcat ttcacgatgg gtcccaaggg agccgccgag
      601 gccgtgaagc tggtttcacc ggaaagagtg gttcctatgc attacggaac tttcttaccg
      661 atgatgactg gtactcctga gcaattcaaa gcagaactaa gagcgctggg ccttgagaag
      721 aaattcgtcc ccatggttgt cggtcaggcc gagcacttct aatgtcaaaa ataacgtcat
      781 tggcgctcgg acttctattt gcgagttgcg taccgcagat ctcttcggca gctgaaatga
      841 aacccggccc ccttgtctta actcggatca atgaaacatt ttatcaagct acggctgccc
      901 tggttgttga ggtcttaact ggagtgggtc attcggtcac agtggtcgac ggctctcata
      961 ccagcgccta tgaggcgatt aaggaaggga cggctgatct ctgcgtcgga ttctggttgc
     1021 cgactggaca tgagaaggcg tggagtcagg tcaaggatt
//
LOCUS       DATMKH010000231         1013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_426876,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000231 DATMKH010000000
VERSION     DATMKH010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426876"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="VNV63_02820"
     CDS             complement(<1..546)
                     /locus_tag="VNV63_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61585.1"
                     /translation="MPQTLDVPVQKPKPLARKARDFASSTFRLLTRKRRGQPDDHNLL
                     PLLIQVLAGFTKVDGRILEDELDSSLGFLRYDYPEAVYSDLRALFQKALKEQQDLGHM
                     AKRLAEEMPQDRKIMLGVQLYDLIAKAGMQQEQVIAYYSFMSQLGMAAQAIDIVYQLN
                     AADTADPSVYQKGTSPLESIVL"
     gene            658..>1013
                     /locus_tag="VNV63_02825"
     CDS             658..>1013
                     /locus_tag="VNV63_02825"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HXC61586.1"
                     /translation="MALLSSFNEDRPVTYFRGYPIYYATILTVAYAVGVLATFFAQAT
                     GNLEAIVSLLAFDTAHSIFGGQIWQIFTYSFVDFPSFFTIIGLLFLYVSAVEIEKYIG
                     RARFLTLYGVIVLIPVV"
BASE COUNT          209 a          266 c          259 g          279 t
ORIGIN      
        1 caggacgatc gattcgagag gcgatgttcc cttctggtag acggaagggt ctgccgtatc
       61 cgcggcattc agctgataaa caatatcgat agcctgggcc gccatcccca attgagacat
      121 gaacgaataa taggcaatga cctgttcctg ctgcatcccc gccttggcaa tgaggtcata
      181 gagctggaca cccagcatga tcttgcgatc ctgcggcatc tcttcagcga gccgtttcgc
      241 catgtgtcca agatcctgtt gttctttcag ggctttctgg aagagggccc gaagatcgga
      301 gtaaaccgct tcgggataat cgtatcgcaa gaatcccagg ctggaatcga gttcatcctc
      361 cagaattctt ccatcgactt tcgtaaatcc cgccagtact tggatcaata gcggcagaag
      421 attgtggtcg tcgggttgtc ctcgtcgctt ccgagtcagg agccgaaagg tcgatgaagc
      481 aaagtctcgc gctttcctcg ccagaggctt tggcttctga accggtacgt ccagggtttg
      541 aggcattacg ggatcgagat tagcttctgt tggaagcaga agcaaacagg ctctgttccc
      601 ttgccgggtg ccttaacttc ctttacaata actcggttcg ctcaacagag atcagttatg
      661 gctttgcttt caagcttcaa cgaggatcgc cctgtcacct atttcagggg atacccgatc
      721 tattatgcga cgattctaac cgtagcgtac gcagtcggag ttctggcaac atttttcgcg
      781 caagccaccg gcaatttgga ggcgatcgtc tcgctccttg cttttgatac ggctcattcg
      841 atttttggcg gccagatttg gcagattttc acctactcct tcgttgattt tcccagcttc
      901 ttcacgatta ttggtctgct gtttctctac gtctctgctg tcgagattga aaagtacatc
      961 ggccgggcgc gcttcctcac gctgtacggt gtgatcgttc tgattccggt ggt
//
LOCUS       DATMKH010000232         1118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1704660, whole genome shotgun sequence.
ACCESSION   DATMKH010000232 DATMKH010000000
VERSION     DATMKH010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1704660"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /gene="nfi"
                     /locus_tag="VNV63_02830"
     CDS             <1..704
                     /gene="nfi"
                     /locus_tag="VNV63_02830"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011309082.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HXC61587.1"
                     /translation="EAIALQRKLAAQVSVENQVETVRYVAGADIATFKDSPQAYAGVV
                     VMSFPGLQIVEEKGLELNLSFPYIPGLLSFRESPLLLKVFEKLEHEPDLILVDGQGIA
                     HPRRFGIACHLGLFLDKPTIGCAKSRLFGHHKESLPERGSSVPLYDNDKDVIGVVLRT
                     KDRTNPLFISVGHQTDLPTAIHYTLACCQGYRLPEPTRQAHLFVGKISKVARGGKAKE
                     PMDEAMSEQLNLPDR"
     gene            complement(779..>1118)
                     /locus_tag="VNV63_02835"
     CDS             complement(779..>1118)
                     /locus_tag="VNV63_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011058225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HXC61588.1"
                     /translation="DPRAIILQKLAQQLFAKYGRSPLYEIAEEIERIGAELLSTKGVY
                     PNVDFYSGTVYEKLGIEPDLFTPVFAMGRVVGWLAHWLEQLKDNELVRPTQIYEGTHD
                     QTYVPIEKRN"
BASE COUNT          252 a          288 c          319 g          259 t
ORIGIN      
        1 gagaggcgat tgcgcttcag agaaaactcg ccgctcaggt gagcgttgaa aatcaggtcg
       61 agacagttcg gtatgtggcg ggggcggaca ttgccacgtt taaagattcg ccacaggcat
      121 acgcgggtgt ggtcgtaatg agctttccag gtttacagat cgtcgaggag aagggacttg
      181 aactgaacct ctcttttcct tacatccccg gccttctctc ttttcgtgaa tccccattgc
      241 tgctgaaggt ttttgaaaag ctggagcatg agccggacct gatcttggta gacggtcaag
      301 gcattgccca tccccgacga ttcggcatcg cctgccacct cggcttgttt ctggataaac
      361 cgacgatcgg ctgtgcgaaa tcgcggctgt tcgggcatca caaagaatcc ctccctgaac
      421 gcggatcatc cgttcctcta tacgataacg ataaggacgt gatcggtgtc gtcctgagga
      481 cgaaggatcg gacgaatccg ctttttatct cggtcggcca tcaaacggac ctgccgactg
      541 cgatccatta cacactggcc tgttgtcaag ggtaccggct tcctgagccc acgcgccagg
      601 cccacctctt tgtggggaag attagcaagg tggcgagagg gggcaaggcc aaggagccga
      661 tggatgaagc aatgtcggag cagttgaacc ttcctgatcg ttaataaaaa aaccgcgagg
      721 cttgcgggcg gctataaatg atcaagcctc ccggtaaaca agattgatga cgttagtatt
      781 agttacgctt ctcgattggg acgtatgtct ggtcgtgggt gccttcgtag atctgtgtgg
      841 gacggacaag ttcgttgtct tttagctgct ccagccagtg ggcaagccac cccaccacac
      901 ggcccatggc gaagaccgga gtaaagaggt ccggttcgat cccgagcttt tcatacacag
      961 tgccggagta aaagtccaca ttgggataga cccctttagt gctgagaagc tcggccccga
     1021 tccgttctat ctcttcagcg atctcgtaaa gcggagatcg gccatacttt gcaaagagct
     1081 gctgggcaag cttctgaagg ataatcgcgc gggggtcc
//
LOCUS       DATMKH010000233         1333 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1070268, whole genome shotgun sequence.
ACCESSION   DATMKH010000233 DATMKH010000000
VERSION     DATMKH010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1333
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1070268"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="VNV63_02840"
     CDS             <1..498
                     /locus_tag="VNV63_02840"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341661.1"
                     /note="catalyzes the NADP-dependent rearrangement and
                     reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to
                     2-C-methyl-D-erythritol 4-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HXC61589.1"
                     /translation="IEARWLFDIPPSRIEVMIHPQSVIHSMVEYVDGSVIAQLGIPDM
                     RAPLSYALSYPDRLALELPPLNLIEIGKLTFLAPDEKRFPCLGYAYEAIRAGGSLPTV
                     LNAANEEAVDAFLKERIKFLDIGKVIRKTMDAYHPDEPKTLENVLEADAWARKKARQV
                     MAKNN"
     gene            524..>1333
                     /locus_tag="VNV63_02845"
     CDS             524..>1333
                     /locus_tag="VNV63_02845"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HXC61590.1"
                     /translation="MIQAIVSVIIVLSILIFFHELGHFLVARRLGVKVLKFSLGFGPK
                     LIGRQIGETEYLLSALPLGGYVKLLGEDPTEIATPEDAARSFSNQSVGRRIAIVAAGP
                     VFNLLLAFLIFAVIFLIGFPNISSQIGDVQKDSPAAKAGLKTDDTVVAIEGKPVEYWD
                     DIKEAVQQYGGKEIALTVEREGAQLALQLTPEEKEAKNLLGDPIKEWRIGVSPKGTFK
                     TKRYGPLNAVIMGAKRTYEVTELTVLGLVRLVQGRIPASTIGGPILIAQLAG"
BASE COUNT          298 a          325 c          410 g          300 t
ORIGIN      
        1 atcgaggcgc gctggctgtt tgatattcca ccgtcaagga ttgaggtcat gatccatcct
       61 cagagcgtga tccattccat ggtcgagtac gtggatggct cggtgattgc ccagctcggg
      121 attccggata tgagggcgcc gctttcgtat gccttgagct atcccgaccg tctggcgctt
      181 gagctcccgc cgctgaactt gatcgagatc ggcaagctca cgtttctggc accggatgag
      241 aagcgctttc cgtgcctggg ctatgcgtat gaagcgatcc gggcgggcgg cagtctgccg
      301 accgttctca atgcggcgaa cgaggaagcg gtggacgcat tcttgaagga acggattaaa
      361 tttttagata tcggtaaagt gatccggaag acgatggacg cctatcaccc ggatgaaccc
      421 aagacattgg agaatgtcct ggaggcggat gcctgggcga gaaaaaaggc ccggcaggtg
      481 atggcaaaaa acaattaatc tctattgaga ggagtaaaaa gaagtgatcc aagcaattgt
      541 ttctgtaatt attgtgctaa gcattttgat ctttttccat gagctgggcc atttcctggt
      601 tgcccgccgt ttaggggtga aggtcctgaa gttttcgctg ggtttcgggc ccaagctgat
      661 cgggagacag atcggtgaga ccgagtatct gctctcggcc cttcccttgg gcggatatgt
      721 caagctcctg ggcgaggacc cgacggagat tgcaacgccg gaagatgcgg cccgttcatt
      781 ctcaaatcag agtgtgggtc ggcgaatagc gatcgtagct gcggggccgg tctttaacct
      841 ccttctcgcg ttcctgatct ttgcggtgat cttcttgatc ggttttccga atatatcctc
      901 tcagatcggt gatgttcaga aggactcccc ggcggccaag gccggtttga aaacagatga
      961 taccgtggtc gcgattgagg ggaagcctgt ggagtactgg gatgacatca aagaggcggt
     1021 tcagcaatac ggcggaaagg agattgcgct gacagtggaa cgcgagggag cgcagctggc
     1081 tctccagctc acgccagaag aaaaagaggc gaaaaacctt cttggagatc cgatcaagga
     1141 gtggcgaatc ggtgtctctc caaaagggac tttcaagacc aagcgatacg gtcccctgaa
     1201 tgccgtgata atgggggcca agcggacgta cgaagtaacc gagctcactg tgttgggtct
     1261 cgtacgtctg gttcagggcc ggatccccgc aagcacgatc ggcggcccga tcctaatcgc
     1321 ccaactggcg ggc
//
LOCUS       DATMKH010000234         1055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1070325, whole genome shotgun sequence.
ACCESSION   DATMKH010000234 DATMKH010000000
VERSION     DATMKH010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1070325"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="VNV63_02850"
     CDS             complement(<1..199)
                     /locus_tag="VNV63_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61591.1"
                     /translation="MATVPATIEELKQQTLADNSAGSIHAQVDEVTSKRTKDQKPYLE
                     IHLRDATANFPLRVWSDHPSFG"
     gene            488..>1055
                     /locus_tag="VNV63_02855"
     CDS             488..>1055
                     /locus_tag="VNV63_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61592.1"
                     /translation="MVAMIVKFARALFARWILWTVSLTVLLQVCQAVDSPSNSSYQRV
                     AANLDAGGVIYLYWSAEKALGELDKKLETVRDLADSDPSLSPDEKNNLRKNFDLGIRL
                     ILHSGLERVKAFGLSSREIEPGLFLNKSYTYLPDRGGFLWDSFAKVPHDFPFLRMVPE
                     NTEGFAFFDFDLAVFWGAVSRELIASEIP"
BASE COUNT          229 a          268 c          285 g          273 t
ORIGIN      
        1 atccgaagga gggatgatcg ctccagactc gcaatgggaa attggccgtc gcatcccgca
       61 agtgtatttc gagataaggc ttttgatcct tggtccgttt ggaggtgact tcatcgacct
      121 gcgcatggat ggaaccggca gaattatcgg cgagagtctg ctgttttaat tcttcaatag
      181 tggcaggaac ggtggccatg ccttgttatc cgacggcctc gccggacatc caatcaaaat
      241 atgccatcga cctaccgaga gcctccgccc taccgggaga atcgccttac tgggcgcctt
      301 tatcctgggc aaacataaaa gggcgctcgg gcctgcaggg ctggaaaaac atccccgctg
      361 accgccgtca acgtgatacg cctgtcgacc atgttccttg tgaagacgac ccttttttgt
      421 tggacggttc tgaaggtgaa acctgtagct cgcgctagcc ttcgttcgcc ggattgctat
      481 agttggtatg gttgctatga ttgtgaaatt cgctcgagcg ttattcgccc gctggattct
      541 atggaccgtc tccctaacgg tgctgcttca ggtctgccag gctgtagatt ccccctccaa
      601 ttcgtcctat caacgtgttg ccgcaaacct cgatgcaggc ggcgtgatct atctctactg
      661 gagcgccgaa aaagcgctgg gagaattgga caagaaattg gaaactgtcc gggatttggc
      721 ggactccgat ccaagtctgt cgccggatga aaaaaacaat ctgcggaaaa actttgatct
      781 gggcattcgc ttgattctcc acagtggcct cgagagggtc aaagctttcg gattaagttc
      841 acgcgagatc gagccgggtc tatttcttaa caagagctat acctatcttc cagatcgagg
      901 cggattcttg tgggactcat ttgcgaaagt gccgcacgat ttcccgttcc tgagaatggt
      961 tccagaaaac accgaggggt ttgcattttt tgattttgac ctcgcggtct tctggggagc
     1021 tgtctctcga gagttgattg cctcggagat tccag
//
LOCUS       DATMKH010000235         1553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2673356, whole genome shotgun sequence.
ACCESSION   DATMKH010000235 DATMKH010000000
VERSION     DATMKH010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2673356"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..766
                     /locus_tag="VNV63_02860"
                     /pseudo
     CDS             105..766
                     /locus_tag="VNV63_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005419461.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          403 a          384 c          417 g          349 t
ORIGIN      
        1 cgcactcctg atgggattgt gtcgcccgtg ccgaacgttc agcggcgtcg tcagccatgc
       61 gcttggttaa cgtgaatcac atgcacttgg ccactacgct ccagaaggaa ccgccgatcc
      121 ccacgaagca cggttttgat gaattctttg gtaatctcta tcatctcaat gcagaggagg
      181 aagccggaga atccggacta tcccaaagat cgagaattcc tcaaacgctt tgggccacgt
      241 gggggtcatc catgcttacg ccgacggcaa gaccgaggac accggtccgc tgaccaaaaa
      301 gcggatggaa acagtcgatg aagaatctct ggcggcggcg ctggatttca tcgatcgcca
      361 gcacaaggcc ccttgcgtga ttcgttggcc gggagtgatc aaacccggca cggtttacaa
      421 cggcatggct gctcatgagg atttcatccc caccttttgt gctgccggtg gcgaccctga
      481 tgtcgtagac aaatgtctta agggttatca ggttgggaac aagacattcc acgtccacct
      541 tgacggctac aacttgatac ccttcttcaa agatgagttg aaggaatcgc ctcgcaacga
      601 attcctttac tggagtgacg acggagacct gttcgccgtg cggatcaaac gttggaagat
      661 ggtcttcaag gagaacaacg aagaagaagc gaacatctgg aggaaggagt tcaccagttt
      721 gaggggaaca agaatcttcg accttctggc cgatcccttc gagcgtcttt ttttgggtct
      781 agcgcgagca ctgaccggcc atgccttcat tcggtccgcc aagtacggga ccaagactcc
      841 tatgccagct tctaaatcaa atccagtgct aatcggtaga aaatatggta attatattca
      901 gctgacgagc tctgataatt acaccgacgg caagaaggtg gcggacatcc ttagatcaaa
      961 gtttgcggcg atcaatcaaa cgactgcgcc taagcgacca aacgaagggc taggtaggac
     1021 cgaagaagga atggagttag cgcgggtgga ggtttccaaa ttatatgaga cccttgagtc
     1081 aagcgtctgc gaactgcgtg gagaactact gccgctccct gttacgaagg ctcgtactga
     1141 aacggaccat ttcattatgg tacatggtcc gagtacatcc gggggaagac tcactctgcg
     1201 cttcctcgct gagggcttca actggaacgc cacggacgtg tcattggaaa ggttgatcgg
     1261 ctccgatgaa aaatttgaca gtggcaggaa aatcttaagg ctccgttatt ccctatcttt
     1321 tgacgagaat ggtcaggtgc tatggaactc aaatcaaact gtcttcaatt ccgagcaggt
     1381 cataacagag gctttattga aattcgccga ttcaataagc gacctgttta aaggtcgtca
     1441 aatctcctaa gaacctaaga aagccaccga ctaaaagagg catttggcga atggggctgt
     1501 tccgggctag cgggagagat gccgccgacc tccatcatag tgtgagtggg gac
//
LOCUS       DATMKH010000236         1073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1390991, whole genome shotgun sequence.
ACCESSION   DATMKH010000236 DATMKH010000000
VERSION     DATMKH010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1390991"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(438..>1073)
                     /locus_tag="VNV63_02865"
     CDS             complement(438..>1073)
                     /locus_tag="VNV63_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXC61593.1"
                     /translation="VLLDEIEKAHPDVFNILLQVFEDGQLTDGLGNTVDFKNSIIIMT
                     SNIGARHLQRKQGLGFQSDREDLLMDKMEELVKNEVKRTFNPEFLNRLDEVIIFLSLS
                     DADLIQILELLVQQLNANLIQKAITLSVTEDAKKWILEKTLVDRTYGARPLRRALQRY
                     VEDPLSEALIAGGISERPAFLEVYLDNNQLFYRPVSEVGEDKSTGILLFSN"
BASE COUNT          238 a          312 c          272 g          251 t
ORIGIN      
        1 gctgtgtcca gtgcagcgtg gcaggaaatc cgggttcgct cgggatgaca aagttgaggg
       61 tggttctgtt tattcagatc gggttatcgg gatggacaga tcaggacaca acaagtgcca
      121 ttgcagctct ctcagccact atccaacttc tcatggaaac acaaccccac cctttgtcat
      181 cccgagcgga ctccggattt cctattactg tgctctcaaa acgaccacgt atgcggcttt
      241 ccctaaagaa agccgcatga ggttcaccga aaccactgag cctgacagga aatccggagg
      301 aagccgaggg acctgctgtg cgcccttccc ctaacaactc cccgtgtact ctaccttcca
      361 accccgcgtt tgttgcttgt gtggatcaaa acaaaaaaag gccggggctt tcgccccggc
      421 ctttcgcttt gcttacgtta attgctgaag agcaggatgc cagtggactt gtcttctccc
      481 acttcgctta ccggccggta gaagagctga ttgttgtcga gatatacctc gaggaacgca
      541 ggacgctcgg aaataccgcc ggcgatcaat gcttccgaca gcggatcttc gacatagcgc
      601 tgcagcgcgc ggcgcaacgg acgcgccccg tacgtgcggt cgaccaatgt cttttccaga
      661 atccacttct tggcatcttc ggtcaccgac aacgtgatcg ccttctgaat cagattggcg
      721 ttcagctgct gtaccaacag ctccagaatc tggatcagat ccgcatccga aagcgacagg
      781 aagatgatga cctcatccag tcgattcagg aattccgggt tgaaggtccg cttgacctcg
      841 ttcttgacca gttcctccat cttgtccatc agcaggtctt ccctgtctga ctggaagcca
      901 agcccctgct tccgctgcag gtgccgtgcg ccgatgttcg aggtcatgat gatgatcgag
      961 ttcttgaagt ccacggtgtt gccaaggcca tcagtcagct gaccatcttc aaaaacctgc
     1021 aggaggatgt tgaagacatc agggtgcgcc ttctcgattt catccagcag cac
//
LOCUS       DATMKH010000237         1065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2032383, whole genome shotgun sequence.
ACCESSION   DATMKH010000237 DATMKH010000000
VERSION     DATMKH010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2032383"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..>1065
                     /locus_tag="VNV63_02870"
     CDS             18..>1065
                     /locus_tag="VNV63_02870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HXC61594.1"
                     /translation="MKLENPVDFAGAIEGNQDELGRRLRRVERQEWILWGTAVTITLL
                     LTAGIVSFLLPLLRTTETGDAFFNLQTELIGLLAIVLLFDLYTIYQQLQIHRIRRQLS
                     ESQQVFRLISENAADMIAVVDVEGRRIYNSQSYERVLGYSPEDLQRSSAFEQIHPEDR
                     ERVREASLEARRTGIGSTLEYRMRHKNGSWRVLESTARVIPSASGVPEKMVIVNRDIT
                     ERKRAEEALRVSEASFRSVVEDAPYGIYQADDEGRFLRVNPALQRMLGYSTTDELQNV
                     NLEREIFRRLEDFRGMRETLHSQDEFKELEIEWRKKGGSPITVRCSGRRVREENGGSA
                     FHEVFAEDVTDKRVL"
BASE COUNT          261 a          243 c          322 g          239 t
ORIGIN      
        1 actaccatgg gagcatcatg aaactggaaa atcccgtcga ttttgcaggg gcgatagaag
       61 gaaaccagga tgagctcgga cggcgccttc gccgagtcga gcgtcaagag tggatcctct
      121 ggggcacggc agtaacgatt acccttctct tgacggctgg gattgtgtcc ttcctattgc
      181 ccttgttacg gacgacggaa acgggagatg cgtttttcaa tttgcagacg gagttgatag
      241 gtctcctggc gatcgttttg ctctttgatt tatataccat ttatcagcaa ttgcagatcc
      301 accgcatccg gcgccaactt tcggaaagcc agcaagtgtt tcgtcttata agcgaaaacg
      361 ccgccgacat gattgcggta gtggatgtcg agggtcggcg catctacaac agccaatcct
      421 atgagagagt tttggggtat tcccccgagg acctccagag gtcatctgct ttcgagcaga
      481 tccatccgga agaccgtgag cgggtgaggg aggcttccct tgaagcgcgg aggacgggaa
      541 ttggaagcac tctcgagtac agaatgcgcc acaagaacgg gtcttggaga gtgctcgaat
      601 ccactgcaag agttatcccg tcggcgagtg gcgtgcccga aaaaatggtc attgtaaatc
      661 gcgacattac ggagaggaaa agagccgagg aggcgctgcg cgtatcggaa gcgagtttcc
      721 gttccgtggt cgaagatgct ccttacggta tctatcaggc cgatgatgag gggcgttttt
      781 tgcgagtaaa tcctgccctg cagcgcatgc tcggttattc caccaccgat gaactgcaga
      841 acgtcaacct ggagagggag atttttagac ggcttgagga ttttcgagga atgcgagaga
      901 cattgcattc ccaggacgaa ttcaaggaat tagagattga atggaggaaa aagggcggct
      961 caccgatcac tgtacgatgc agcggccgtc gggtgaggga agaaaatggc ggatcggcat
     1021 ttcacgaagt gtttgcggaa gacgtgactg acaaacgtgt tctcg
//
LOCUS       DATMKH010000238         1193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2342900, whole genome shotgun sequence.
ACCESSION   DATMKH010000238 DATMKH010000000
VERSION     DATMKH010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2342900"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1193
                     /locus_tag="VNV63_02875"
     CDS             <1..>1193
                     /locus_tag="VNV63_02875"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HXC61595.1"
                     /translation="DKPPLIYWCQVLSYNVFGENDFAARFPSAVFAALTAVATWIFSS
                     RIFGPRVGLWSGIIFATSLQVFVHARAAVADMPMVFFFLTATWADWERLRNPRATSWW
                     WTFYLSLGLGFLAKGPAALLPVFNVPIHSLLNRTPCHCKLRSGLLGGLVVLIVIGFWG
                     IPALMATNGAYLQIGLGKQVLQRSLQPMESHGGAGLAGYLLFLPFYLVVSFFSFFPWC
                     FFLPGCVKRLLAHREPDENYLLGPILIVLFVFTLIQTKLPHYVLPAYPMLAILVARRV
                     AESKWRRILLGIALVLYLLVALVGFRVIESEFLSKSIAKTALPLISPETRTASLNYDE
                     QSLIWYLREKTRPFHRRLDSSAFSDFMNQPGPALCVVNKESIGQVQLNSEWKTFEVSG
                     HNFAR"
BASE COUNT          225 a          331 c          304 g          333 t
ORIGIN      
        1 gacaaaccgc ctttgatcta ttggtgccag gttctgtcat acaatgtttt cggggagaac
       61 gatttcgccg cacgatttcc ttcggcggtt tttgctgcac tcaccgccgt agcgacctgg
      121 atcttttcga gccggatctt cggcccacgc gtcggtctct ggtcaggaat cattttcgcg
      181 accagtcttc aggtttttgt ccatgcgcgg gcagccgtag cagatatgcc aatggttttc
      241 ttttttctca ctgcgacgtg ggccgattgg gaacggttaa ggaatccgag agccacatcc
      301 tggtggtgga cattctatct ttctctcggt cttggcttcc tcgcaaaagg tcctgctgcc
      361 ttgctccctg tttttaatgt ccccatccat tcgcttctga atcgtacccc ttgccattgc
      421 aaactccggt cgggactgct gggaggactc gtagtcctca tcgtgatcgg gttttggggt
      481 attcctgcgc tgatggcaac gaatggagcg tacctgcaga ttggtcttgg aaagcaagtc
      541 ctccaacgct ccctgcaacc gatggaaagt cacggagggg cgggtctcgc gggatacctg
      601 ctgttcctgc cattttatct ggtcgtcagc ttcttcagct tttttccgtg gtgcttcttt
      661 ctgcccggct gtgtaaagcg gctcctcgcc cacagagaac cggacgaaaa ttatcttttg
      721 gggccgattc taatcgtgct tttcgtgttc actctgattc agaccaagtt accgcactac
      781 gtccttccgg cttacccgat gctcgcaatc ctcgtggccc ggcgagttgc ggaaagcaaa
      841 tggaggcgga tccttctggg gattgccctg gtcctttatc ttctggttgc tctcgtcgga
      901 tttcgagtta tcgagtcaga atttctgtca aagagcatcg ccaaaacagc gcttcctctc
      961 atctccccag aaacgcgaac ggcatcgctc aattacgacg agcaaagtct tatatggtat
     1021 ctgcgagaaa aaacgcgccc ttttcaccga cgcctggatt cctccgcctt ctcggatttc
     1081 atgaatcaac cgggaccggc gctctgtgtg gtgaacaagg aatcgatcgg ccaagttcag
     1141 ctgaattccg aatggaagac cttcgaggta tccggacata actttgcccg ttg
//
LOCUS       DATMKH010000239         1013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1391507, whole genome shotgun sequence.
ACCESSION   DATMKH010000239 DATMKH010000000
VERSION     DATMKH010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1391507"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1013)
                     /locus_tag="VNV63_02880"
     CDS             complement(<1..>1013)
                     /locus_tag="VNV63_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HXC61596.1"
                     /translation="LALSSPFVEWQDNPIVQNRISDGFKSLCFVPLISRNRAIGTLNL
                     GRLQGDAFTEEDLSFIGQVASQIAIAIENTSAYRQVEQARAELEKALGEIEQRTEALR
                     RSEGYLAEAQKLNHTGSWAWDVRTRDAFWSQEMFQIFGYDPEKTKPSLSHFLERVHPE
                     DRPMVEQVVKREMTGSDQDVVSDYRIVLPDRTKKHLHVIAHPVTGESGEVIQVIGTSM
                     DVTEQVEARIKLEKAFAEIKLLKDQLYKENLALRDEVEGLSMFEEVVGSSPALKAVLK
                     SVRIVAPADSTVLIQGETGTGKEMIARAIHKLSPRKEQAFVKVNCAAIPLGLLESELF
                     GHE"
BASE COUNT          220 a          291 c          250 g          252 t
ORIGIN      
        1 tctcatggcc aaacaactcg ctttccagaa ggcccagagg gatggctgcg cagttcacct
       61 tcacaaaggc ctgctcctta cgggggctca gcttgtgaat ggcgcgagcg atcatctctt
      121 tgcctgtgcc cgtctcgccc tggatgagga ccgtcgagtc cgccggcgcg acgattcgaa
      181 cgctcttgag cacggctttc agggccgggg aagagcccac aacctcctca aacattgaga
      241 gaccttccac ttcgtcgcgg agggccaagt tttccttgta aagttggtcc tttaaaagct
      301 taatttcagc aaatgctttt tccagtttga tcctggcttc tacctgctcg gtgacgtcca
      361 tgcttgtgcc aatgacttga atcacttcgc ccgattcacc cgtcaccgga tgggcaatga
      421 cgtgaaggtg ctttttcgtt cgatccggga gaacgattcg ataatcagac accacatctt
      481 ggtcagaacc cgtcatctcg cgtttcacca cctgctccac cattggtcga tcttcgggat
      541 gaaccctttc cagaaagtgg gacagagagg gcttcgtttt ttcgggatca tatccaaaaa
      601 tttgaaacat ctcttgtgac cagaaagcat cacgcgtacg gacatcccaa gcccagctgc
      661 ccgtatggtt caatttctgt gcctcagcaa ggtagccttc acttcgccgg agggcctctg
      721 tccgctgctc aatctctccc agagccttct ccaattcggc tctggcctgc tcgacctggc
      781 gataggctga cgtgttctct atcgcaatgg cgatttggct ggcgacttgg ccaataaaag
      841 acaggtcttc ctctgtgaag gcgtcacctt ggagcctacc tagattcaac gtgccaatcg
      901 ctcgattccg gctgatcaaa gggacgaaac acaatgactt gaaaccatca gaaatgcggt
      961 tttgaacgat cggattgtcc tgccactcga cgaatgggct gctgagtgcc aaa
//
LOCUS       DATMKH010000240         3219 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1075482, whole genome shotgun sequence.
ACCESSION   DATMKH010000240 DATMKH010000000
VERSION     DATMKH010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1075482"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="VNV63_02885"
     CDS             <1..477
                     /locus_tag="VNV63_02885"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222520.1"
                     /note="Catalyzes the reversible oxidation of malate to
                     oxaloacetate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HXC61597.1"
                     /translation="RAFIALELSVSVENIQTLVLGGHGDTMVPLVRYTTVAGIPVAEL
                     IPKDRIETIIQKTRDGGAEIVKLLKTGSAYYAPSAAVTEMVEAIVKDKKKILPCAAFC
                     QGEYGFRDLFLGVPVKIGNGGVEQILDIKLMPEEMSGLKKSAAAVSELCRAVDKMI"
     gene            485..1468
                     /gene="moaA"
                     /locus_tag="VNV63_02890"
     CDS             485..1468
                     /gene="moaA"
                     /locus_tag="VNV63_02890"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HXC61598.1"
                     /translation="MPLIDSFQRRISYLRVSVTDRCNLRCQYCMPVEGVMPSAREDLL
                     SYEEIYRIAACFVRFGVNHVRLTGGEPLVRKKLVKLVSMLASLPGLKDLSMTTNGMLL
                     KQYAPRLAAAGLKRLNISLDSLDSRRFETITRGGRLKDVLAGIDCAIEAGLAPVKLNM
                     VVMRGRNEDELPEFVRLACSRGLTIRFIECMPMADVGLWNRDHFISTDEIKKKIETVY
                     SLEPASMPGSGPAVYFRVRDTGAVLGFISPLTPRFCEICNRVRLTALGRLRLCLDHED
                     HLDLKALLRAGAKDDDLEAEIASAILRKPERNRFLDHTQYVSPGVMSAIGG"
     gene            1638..1835
                     /locus_tag="VNV63_02895"
     CDS             1638..1835
                     /locus_tag="VNV63_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61599.1"
                     /translation="MECPRCQGSMVYEVFEDLRDDTGQLNFQGWRCIICGEILDPTIL
                     SNRELRRGPMVARNRKIMALR"
     gene            2048..2527
                     /locus_tag="VNV63_02900"
     CDS             2048..2527
                     /locus_tag="VNV63_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HXC61600.1"
                     /translation="MSAHAEMKEIEVGQEAPDFTLPDQDKKDVKLSSFRGKKNVVLAF
                     YPLDWSPVCTTENKCFTDDFPKFESVGAEVFGISADSFFSHKAWAESLDLKQRLLSDI
                     HREVSKKYGLYFEPLNCSKRATVIVDKKGIVKFKKVQQDILKARDNQEILEALKKLS"
     gene            2556..>3219
                     /locus_tag="VNV63_02905"
     CDS             2556..>3219
                     /locus_tag="VNV63_02905"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXC61601.1"
                     /translation="MKAQINGLDIAYTDQGKGTAIFFIHGYPLNKTMWESQVKDLSTG
                     FRVITVDLRGHGESSAPLWFCTMEMFADDLRALLDHLSIKKAVIAGFSMGGYAVFAFY
                     RKYRNRVQGLVLADTRPQPDSPEGKQGRFKTAQAAHKDGTNAIAEAMLSKLLTPKSIE
                     TRQDVVQKVRQIMVETPVTGIAADLMAMSERLDSVPLLSEIQCPTLIIVGEQDRLTPP
                     ADA"
BASE COUNT          755 a          790 c          913 g          761 t
ORIGIN      
        1 cgggcattca tcgcgctgga gctttctgtc tcagtcgaga acattcagac gctagtactg
       61 gggggacatg gcgatacaat ggtgccgctg gtgaggtata ccaccgtcgc cggtattccg
      121 gtggccgagc tgatccccaa agaccggatt gagacaatta ttcagaagac ccgggacggc
      181 ggtgcggaga tcgtcaagct gctcaagaca ggaagcgcct attatgcgcc ttccgcagcc
      241 gtgacagaga tggtggaagc gatcgtcaag gataaaaaga agatcctgcc gtgcgccgcg
      301 ttctgtcaag gggagtacgg gttccgtgac ctgtttttag gcgtgccggt gaagatcggc
      361 aacggggggg tcgagcagat tctcgatatc aaactaatgc cggaagagat gtccggtctt
      421 aagaaatcgg ccgcggcggt gtccgagctc tgccgtgccg tggacaagat gatttaggat
      481 tgcgatgcca ttgatcgata gctttcagcg aaggatcagc tacctccgtg tctcggtaac
      541 ggatcgttgt aatttaagat gccagtactg tatgccggtg gagggcgtca tgccatccgc
      601 ccgagaggat ctcttgagtt acgaagagat ttaccggatc gcggcctgct ttgttagatt
      661 cggcgtgaat catgtgaggc tcacgggagg agagccccta gtccgaaaaa aactggtcaa
      721 gttggtttcg atgctcgcga gccttccggg cctcaaagac ctttcgatga ctaccaacgg
      781 aatgctctta aagcaatatg cgccgcggct tgcggctgcc ggcctaaagc ggttgaacat
      841 cagtcttgat tcactggact cgagacggtt tgagacgatc acgcggggcg gcaggttgaa
      901 agacgtcttg gcgggcatcg attgtgcgat cgaggccggc ttggcaccgg tcaaactcaa
      961 catggtcgta atgcggggcc gtaacgagga tgagctgccg gagtttgtga ggctggcctg
     1021 ctcccgcggg ctgaccatcc ggttcatcga atgcatgccg atggctgatg tcggcctgtg
     1081 gaaccgcgac cattttattt ccacggatga gatcaaaaaa aagattgaaa cggtctattc
     1141 cctggagccg gccagtatgc cgggcagcgg cccggcggtc tatttccgag tccgagacac
     1201 cggggcggtc ttaggcttca tcagcccgct caccccgcgg ttctgcgaaa tctgtaaccg
     1261 tgtgcgctta accgcactcg gcaggcttcg gctctgtctc gatcatgaag atcacctgga
     1321 tctcaaggcc ctccttcgcg ctggtgcgaa ggacgatgat cttgaggccg agattgcttc
     1381 cgccatcctc cgaaagcccg agaggaaccg gtttctcgat cacacccagt acgtttcgcc
     1441 cggtgtgatg tccgccatcg gcggataatc tgccacaaat aaccgtttag ctggtacgga
     1501 agacctattg gtctaggtct tttgcttttg gtgtttggga cttctggtgg attgtttcct
     1561 ctcgatctgg gcagtatact cctgatcata aaacgggagg atcttcccca tttttagtta
     1621 gaaggaggtt gttcaagatg gaatgcccac gatgccaggg cagtatggtt tatgaggtct
     1681 tcgaagatct gagagatgat accggccagc tcaattttca aggttggcgg tgtatcatct
     1741 gcggtgagat ccttgatccg accattctgt ccaatcggga attacggcgc ggcccgatgg
     1801 tggcgcgcaa tcgcaaaatc atggccctcc ggtagcctta taaccacaag gtcccgctta
     1861 tccatggccg tacgttattt gataaccata taacgatggc ctttttatgc cttgacaaaa
     1921 tcagtccgac tatggtaatt aattcctccc gatacggtca attttaaaac aatcaactta
     1981 ccgctcgcca aaagcaatcg gcgggtatcc catttaacaa tgaaatgaga cgaaaggaga
     2041 atccatcatg tcagcccatg cagagatgaa agagatcgag gtggggcagg aagccccgga
     2101 ttttacgctt ccagatcaag ataaaaaaga cgttaagcta agcagttttc gaggcaagaa
     2161 gaatgtggtg ttagcttttt atcctttgga ctggagcccc gtctgtacta ctgagaataa
     2221 atgcttcacg gatgacttcc ccaaattcga gtccgtcggg gccgaggtat tcgggatcag
     2281 cgcagatagc ttcttttcgc ataaagcttg ggcggaatct cttgatctca aacaacggct
     2341 attgtccgat attcatcggg aagtctccaa gaagtatggt ctttattttg agcctttgaa
     2401 ctgttccaag cgggccaccg tgatcgtgga taaaaaaggt attgtgaaat tcaagaaggt
     2461 ccaacaagac attctcaagg cgagggataa tcaagagatc ttggaggcgc tcaaaaagct
     2521 gagctgatct tttctctttt gaagagagta aatccgtgaa ggcgcaaatt aacggcctcg
     2581 acatcgctta taccgatcag ggaaagggta cggccatctt cttcatccat ggctatcctt
     2641 tgaataagac gatgtgggaa tctcaggtca aagatctctc aacaggattt cgggtaatta
     2701 cggtagatct gcggggccat ggagagtcca gcgccccgct gtggttttgt acaatggaga
     2761 tgtttgcgga tgacctccgg gccctgctgg atcatctctc gatcaagaag gccgtgatcg
     2821 ctggtttctc aatgggcggt tatgcggtct ttgcctttta ccgaaagtac cggaaccggg
     2881 ttcagggatt ggtcctggca gacacccgtc cccagccgga cagccctgaa gggaaacagg
     2941 gacgatttaa gactgctcaa gccgctcata aagacgggac gaatgcgatt gcggaagcaa
     3001 tgctttccaa gctcctgacc ccgaagtcaa tcgagacacg ccaagacgtc gttcagaagg
     3061 ttcggcagat catggttgag acgccggtga ccggtattgc ggcagacctg atggccatgt
     3121 cggagcgact ggattcggtg ccgctcttgt ccgagatcca gtgtccgacc ttgatcattg
     3181 tcggcgagca ggacaggctg acgccacccg cagatgcaa
//
LOCUS       DATMKH010000241         1134 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4590268, whole genome shotgun sequence.
ACCESSION   DATMKH010000241 DATMKH010000000
VERSION     DATMKH010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4590268"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(213..806)
                     /locus_tag="VNV63_02910"
     CDS             complement(213..806)
                     /locus_tag="VNV63_02910"
                     /inference="COORDINATES: protein motif:HMM:NF015444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF296 domain-containing protein"
                     /protein_id="HXC61602.1"
                     /translation="MNKRLVSRRHLLISGLVGCSAMMTGVALPSSEEPPSGYIRPGPI
                     SARGKAPGLKTRLLSHQDARTFALIFAKNDEVMAGLTEFAETNHLAASHFTAIGAFQS
                     ALLGWFDSEKNAFREVPITNQVEVASFSGDIGLINGKPAVHAHAVVAFPDGTTRGGHV
                     ITATVWPTLELFLTDEPAALVKEEDPETGLYLFDPRA"
BASE COUNT          278 a          314 c          285 g          257 t
ORIGIN      
        1 gctgtctctt cggcgactgc agccaatggc aaaactgatc cgcctgctaa accgagggcg
       61 actgtgcctg atgcgcgctt aataaatcct cttcgagaaa tatccataac tctggtcctc
      121 caattcctaa aggtagctaa acacggttgc tgctggctgc acccactttt tttgcgacct
      181 ttgcgatcct ttgcggctac ttcctttgcg gatcaggccc ttggatcgaa aagataaaga
      241 cccgtttcag gatcttcttc cttgaccagg gcagcaggct cgtccgtcaa aaacagttcg
      301 agtgtaggcc agaccgtggc cgtaataaca tgccccccac gcgtggtccc atccggaaaa
      361 gcaactaccg catgagcatg aaccgctggc ttcccattaa tcaatccgat gtctccgctg
      421 aaagaggcca cctcgacttg gttcgtaatc ggaacctccc gaaacgcatt cttctcgcta
      481 tcaaaccatc ccagcaaagc gctttgaaaa gcgccgatag ctgtaaaatg actggccgcg
      541 agatgattgg tttcggcaaa ttccgtcagc ccagccatca cttcatcatt ctttgcgaaa
      601 atgagagcga aggttctggc gtcctgatgt gaaagcagcc gggttttcag gcctggggcc
      661 tttccccgtg cgctgatcgg gcccggacgg atataaccgg acgggggttc ctcggaagac
      721 ggtagggcta caccagtcat catggcagag catcccacca ggccggagat caataaatga
      781 cgacgcgata ccaaacgttt gttcataaat agctcaaggt tactgaaagg cttctctgac
      841 ttccggctca ttggcctgag atgccatgct catgacgaat ggtctttttt tattccaaag
      901 agcgatggca gcccttcctc agccgaagaa gtgtcaagga ttcggtgaaa agtgaaacgc
      961 ttctgcctcg cccggcctaa gcggagatcg gagagttcac gagaaaacga tctgttatgg
     1021 accaagaaat ccaacgacaa agaagctcag gtgcgagtaa cgcttcctcc actgggatga
     1081 gccgtgcagg ccgatctcaa cggcaaggcc gatctagctt tatttgggcg accg
//
LOCUS       DATMKH010000242         1256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3622576, whole genome shotgun sequence.
ACCESSION   DATMKH010000242 DATMKH010000000
VERSION     DATMKH010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3622576"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="VNV63_02915"
     CDS             complement(<1..556)
                     /locus_tag="VNV63_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006893120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HXC61603.1"
                     /translation="MCGICGKLNFEKGARVEPDLIRAMMDAIRHRGPDDRGIYTASEV
                     GLGFVRLSIIDLSSGHQPLSNEDGTVWIVFNGEIYNYRELRSFLLSKGHVFKTQSDTE
                     VIVHLYEELGPQCLEKLRGMFAFAIWDEKSQSLFLARDRVGIKPLYYSLTDKALVFAS
                     EIKAILADPAISRELAPEIIDRFLT"
     gene            complement(589..>1256)
                     /locus_tag="VNV63_02920"
     CDS             complement(589..>1256)
                     /locus_tag="VNV63_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXC61604.1"
                     /translation="RGFRKSGGGVMEAFYDGYHAIQCNDWDFIVKLDGDLTLPGNYFE
                     NCFEHFELDPELGIGGGEIYHDLEGVQKLEANPKFHVRGATKIYRRACWEAIGGLLKA
                     PGWDSIDEVKANMLGWRSYSFEELRVVHHRFTGSADGLLRDRVKHGVACYVSGYHPLF
                     VAASCFYRLRQQPYVIGSAAICYGFLKAYFTRIPRVDDPQMIKYLRAQQLRRLCGLET
                     IWK"
BASE COUNT          306 a          360 c          293 g          297 t
ORIGIN      
        1 aggtcaggaa ccggtcgata atctcggggg cgagttcccg gctgatcgcg ggatccgcta
       61 ggattgcctt gatctcggaa gcaaacacca atgctttgtc ggtcagcgag tagtagagag
      121 gctttattcc aacccggtcc ctcgccagga agagagactg actcttctca tcccatatgg
      181 cgaaagcaaa cattccgcgg agcttctcca ggcattgagg gccaagctcc tcgtaaaggt
      241 gcacgatcac ctcagtatcg ctctgagttt tgaaaacatg tcccttgctc aggagaaaac
      301 tgcgcaactc acggtaattg tagatctcac cgttgaacac aatccagaca gtgccgtctt
      361 cattcgagag cggctggtgg ccgctgctaa gatcgatgat cgagagccgc acgaaaccca
      421 agcccacttc agaagctgtg taaatcccac ggtcgtcggg gcctcgatgg cgaatcgcat
      481 ccatcatggc tcgaatcaga tcgggctcga ccctggcgcc tttctcgaag tttaattttc
      541 cacaaattcc gcacatatcg attatttgtt tccgactccg gactgcggtc acttccagat
      601 tgtttctagc ccgcagagac gtctcagttg ctgcgcccgc aaatatttga tcatctgtgg
      661 atcatccact cgcgggatac gggtaaaata agccttcagg aatccgtaac aaattgccgc
      721 ggagccgatg acataaggct gctgcctgag cctgtaaaag cagctcgccg cgacaaaaag
      781 cgggtgatat cccgatacgt aacaggctac accgtgcttt accctgtcgc gcaatagtcc
      841 gtcggcggat cccgtaaagc gatgatgaac aacacggagt tcctcgaagc tgtaactcct
      901 ccaacccagc atgttggctt ttacttcatc aatactgtcc caacccggag ccttcaataa
      961 accgccaatc gcttcccaac aggctctcct atagatcttc gttgctcccc ggacatggaa
     1021 ttttggattg gcttcaagtt tttgtactcc ttcaagatcg tgataaatct ctcctccgcc
     1081 aattccgagc tcagggtcga gctcaaaatg ttcgaaacaa ttttcaaaat aattcccagg
     1141 caaagtcagg tccccgtcga gcttcacgat gaaatcccaa tcattgcact ggatcgcatg
     1201 gtagccgtcg tagaatgctt ccatcactcc gccgccagac ttgcggaacc ctctgt
//
LOCUS       DATMKH010000243         1300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4283477, whole genome shotgun sequence.
ACCESSION   DATMKH010000243 DATMKH010000000
VERSION     DATMKH010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1300
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4283477"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="VNV63_02925"
     CDS             complement(<1..308)
                     /locus_tag="VNV63_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008354030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61605.1"
                     /translation="MKGVPNWFMVLWCVLMPVTSFLLIPSIQGTIPAYFLAFLSVLFV
                     ILGRNDRQPHVQRIQYITITLVVGAVWLLLLCGSQLGHLFSDRRDFGDMFLNDPGNPK
                     V"
     gene            complement(374..>1300)
                     /locus_tag="VNV63_02930"
     CDS             complement(374..>1300)
                     /locus_tag="VNV63_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61606.1"
                     /translation="GMSRAEIKGKFDEIVAFAAVEKFLDTPVKRYSSGMYVRLAFAVA
                     AHLEPEILIVDEVLAVGDSEFQKKCLGKIREVATGGRTILFVSHNMQAVSVLCNRGMF
                     LREGSLEYIGSIKEAIDMYTASFVHANGQEDNPDRRRGTGEYRFTGAGPTRQFFEGAE
                     EKVIQFEIQRRGTQLVGRMWVSAMIVDSNGVVVAQCDSRLLGFSVEDFPLINGKLRFT
                     TPWLKPGTYRVDLYVGAHWIMDIWEGACSLIISPVLPYPNSSSPSASASGLVFADFSF
                     EASEAKEEGISSTLPNYENGDSLKIGYSALKV"
BASE COUNT          341 a          351 c          321 g          287 t
ORIGIN      
        1 acctttgggt tcccggggtc gttaaggaac atgtcgccaa agtcgcgcct atctgagaag
       61 agatgcccca gttgactccc gcagagcagg agaagccaga ctgctcccac cacgagagta
      121 atcgttatat actgaatccg ctggacgtgt ggttgcctgt cgttgcgacc tagaatcaca
      181 aagagaacgg aaagaaaagc aagaaaatat gccgggatgg ttccttggat ggaagggatc
      241 aataaaaaag aggtgaccgg catcaaaacg caccaaagca ccatgaacca attaggtact
      301 cctttcattg gactagagag tagattgatg gcattggtcg tttttcgctg gtcgggactg
      361 actacggaag gacttacacc tttaaggctg agtacccaat tttgaggctg tcgccgttct
      421 catagttcgg aagggtgctg cttatgccct cttctttggc ttcagaagct tcgaaagaaa
      481 agtccgcaaa aaccagtccg gatgctgaag cactaggaga ggaagagttc ggataaggca
      541 gaaccggaga tatgattagc gaacaagcgc cttcccaaat atccatgatc cagtgagccc
      601 cgacatacag gtcaacacga taagtcccag gttttaacca aggcgtcgtg aagcgaagtt
      661 ttccgttgat cagcgggaaa tcctcgaccg agaatcccag taggcgtgaa tcacattggg
      721 caacaacgac tccgttggaa tcgacaatca tggcgctgac ccacattcgg ccgaccaact
      781 gcgttccccg gcgttgaatc tcaaactgaa tgactttctc ttccgccccc tcgaaaaatt
      841 gtcgggtggg accggctccg gtgaaacgat attcaccggt gcctcgtcgg cgatccggat
      901 tatcttcctg accgttggca tggacaaaac tcgcggtgta catatcgatc gcttccttta
      961 tgctcccaat gtattccaag gagccctcac gaagaaacat cccccgatta caaaggaccg
     1021 ataccgcctg catattatgg ctaacaaata aaatggtccg accaccggtg gccacctccc
     1081 gaatctttcc gagacacttt ttctgaaact ccgaatctcc caccgccaac acttcgtcga
     1141 cgatcaaaat ctcaggttcc agatgggccg cgaccgcgaa ggctagccgt acgtacatgc
     1201 cgctgctgta ccgcttgacg ggagtgtcga gaaatttttc caccgccgcg aaggcgacaa
     1261 tttcgtcgaa cttgcctttg atctcggccc ggctcatccc
//
LOCUS       DATMKH010000244         2565 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2349745, whole genome shotgun sequence.
ACCESSION   DATMKH010000244 DATMKH010000000
VERSION     DATMKH010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2349745"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="VNV63_02935"
     CDS             complement(<1..181)
                     /locus_tag="VNV63_02935"
                     /inference="COORDINATES: protein motif:HMM:NF014617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HXC61607.1"
                     /translation="MSLDLINRIGEIARPILDSMGLELVEVEYSGHGGRGRVRFFIDK
                     SGGVTLDECEQASRSI"
     gene            complement(274..1119)
                     /gene="dat"
                     /locus_tag="VNV63_02940"
     CDS             complement(274..1119)
                     /gene="dat"
                     /locus_tag="VNV63_02940"
                     /EC_number="2.6.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR01121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047810 - D-alanine:2-oxoglutarate
                     aminotransferase activity [Evidence IEA];
                     GO_process: GO:0019480 - L-alanine oxidation to pyruvate
                     via D-alanine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino-acid transaminase"
                     /protein_id="HXC61608.1"
                     /translation="MPNIGVINGKFMPLADARVSVEDRGFLFGDGIYELIRSHEGQLF
                     GLDEHLRRMERSAQAIRLDLRHSRKEWKRLITKAVEQSRIPEAKIYIEVTRGAAPRNH
                     TFPKGTASTTVITVRRFEPLPERFRRDGARTITAPDLRWGRCDIKSLNLLPNILAKQQ
                     ALESGAVEAIFIRSGQVTEGTSSNVFAVYGDAVVTPPQGPSILSGVTREMVLELVRSQ
                     GLKAEERAMSGLDLLQADEVFLTGTTMEVLPVSLVDQATIGKGKPGRTTQQLYLRLQE
                     MMRRP"
     gene            complement(1122..1766)
                     /locus_tag="VNV63_02945"
     CDS             complement(1122..1766)
                     /locus_tag="VNV63_02945"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXC61609.1"
                     /translation="MDLYARQRRYFAGAYETGEHGWPTTEPSDFVVKVFKKIKPRLSR
                     TRLLDIGCGEGRHTLLAARYGFHTVGLDYQPPAIHRALRFAKEQGVTTGFSFLIGDVF
                     HLPFQEKSFDILIDFGCLHHVRKKDFSKYLDSILTLLGPGGYYVLSCFSTQFKHHAGE
                     RRTRDWLVHRGHYDRFFRKQDLNALFGRDFDQLDYNRERDGSYVFHQVLMRRKN"
     gene            2143..>2565
                     /locus_tag="VNV63_02950"
     CDS             2143..>2565
                     /locus_tag="VNV63_02950"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HXC61610.1"
                     /translation="MQKWLLLAFLIFFSVASASAGSETGRQEQALRKITFSTGTTIQA
                     EVADTEAKRQLGLMFRKSLPQDRAMLFIFDRPDLYYFWMKNCLFPIDMVWLDQKKRIV
                     NVTSNVPPCLSDPCPTYQPASKALYVIETVGGFANENGL"
BASE COUNT          598 a          723 c          643 g          601 t
ORIGIN      
        1 cgatggaacg gctcgcctgc tcacattcat cgagggtcac acccccggac ttgtcaatga
       61 agaaacgaac ccgaccccgc cctccatgcc cggaatactc cacctctacg agttccagtc
      121 ccatggaatc cagaatgggc cgtgcaattt ctccaatacg attaatgaga tctaaagaca
      181 tactccttct caagcaaatt aaaaaagtgg gccgactacg cccactttcg agggataaga
      241 tagcacaata aattcctgaa tgcaagagga aaatcaggga cgacgcatca tctcctggag
      301 tcggagatag agctgctgcg tggtccggcc gggtttcccc tttccgatcg tggcctgatc
      361 caccaggctc accggtaaga cctccatcgt ggtaccggtg aggaagacct catcggcctg
      421 aagaaggtcc agaccgctca tcgcacgttc ctccgccttc agcccctggc ttcgtacaag
      481 ctcaagcacc atttcccgcg tcacaccgga gagaattgac ggtccctgag gcggggtcac
      541 gaccgcgtcg ccatacacgg caaacacgtt cgagctggtt ccttcggtca cctgaccgga
      601 tcgaataaag atcgcctcaa ccgccccgga ctccagcgcc tgttgcttgg ccagtatgtt
      661 cggcaggagg ttgagcgatt tgatgtcgca acgtccccat cggagatcag gcgccgtaat
      721 tgtccgagct ccatctcttc ggaaccgctc cgggagaggc tcgaaacgac gaacagtgat
      781 gacggttgtg gaggccgttc ctttcgggaa ggtatgattt cgaggcgcgg caccccgggt
      841 cacttcaata taaatctttg cctccggtat cctgctctgt tccaccgctt ttgtgatcaa
      901 gcgcttccat tctttccggc tgtgtcttaa atccaaacgg atcgcctggg cgctccgttc
      961 catccgtcga agatgctcat cgagcccaaa gagctgtccc tcgtggcttc ggatcagctc
     1021 atagatcccg tctccaaaaa ggaagccgcg atcctccacc gacacccggg catcggccag
     1081 cggcatgaac ttcccattga tcactcctat attcggcatg gtcagttttt tctcctcatc
     1141 aagacctggt gaaacacata agacccgtcg cgttcccggt tgtaatcaag ctggtcaaaa
     1201 tcacggccga acagggcgtt taaatcctgt tttcgaaaga accggtcata atgtccccga
     1261 tggaccagcc agtctctggt tcgcctctca ccggcatgat gtttgaactg cgtcgagaaa
     1321 caggacagga cataataccc gccaggcccc agcaatgtta gaatcgagtc gagatatttt
     1381 gaaaagtcct ttttcctgac atgatgaaga catccaaagt cgatcagaat atcaaagctc
     1441 ttttcttgaa acgggagatg gaacacgtct ccgatgagaa atgaaaatcc ggtcgtgacg
     1501 ccctgctcct ttgcaaacct caaggcccgg tggatcgccg ggggctgata gtccagaccg
     1561 accgtatgaa atccgtatcg agcggccaga agggtgtgtc ttccctcccc gcatccgata
     1621 tccaggagcc tggttcggga aagccggggt tttatttttt taaaaacctt tacgacaaag
     1681 tcggacggct ctgtcgtcgg ccagccgtgc tctccggtct cataggcccc tgcgaaataa
     1741 cgcctttgtc gggcatagag atccataaag caagtctatt gtaaactgtc cgagcggtca
     1801 agtggtttgt gagagccata gatcgatttt tcgtcaggct gtctggcctc gccagaagat
     1861 tgcttcgcca tctgcaagga aaaattaaat tgatatgctg cacctttggt gcagagcagt
     1921 gtcattcgca caccctatgc gtccaaggaa attgagtctt ctgcagcttt cgcgtcggcc
     1981 agggacgcag ccgttactgc atccctggcg gtggtttgcc tttttgattg ttcttgagcg
     2041 cagggtctgc tccttcacct ctggcttcgg ccaggaaaaa ctaagtcagc caaggtttgt
     2101 acggcaattg acacccggct ggcttccgac taaaatgatt ttatgcaaaa atggctgctg
     2161 ttggcttttc tgatattctt ctcggtcgcg tcggcttctg cgggaagcga aaccggccga
     2221 caagaacagg cgcttcgtaa gattacattt tccacgggta caacgataca agcggaagtg
     2281 gcggatacgg aggcgaaacg gcaattgggg ctgatgttcc gtaaaagtct tcctcaagac
     2341 cgcgcgatgc ttttcatctt cgatcggccg gatctttact acttctggat gaaaaattgc
     2401 cttttcccca ttgacatggt ctggctcgat caaaaaaaac ggatcgtaaa cgttacgtcc
     2461 aacgtccccc cttgcctcag cgatccctgt ccgacctatc agcccgcaag caaggcgtta
     2521 tacgtgatcg aaacagtggg cggcttcgca aatgaaaacg ggctg
//
LOCUS       DATMKH010000245         1043 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1398257, whole genome shotgun sequence.
ACCESSION   DATMKH010000245 DATMKH010000000
VERSION     DATMKH010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1398257"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            30..761
                     /gene="hisA"
                     /locus_tag="VNV63_02955"
     CDS             30..761
                     /gene="hisA"
                     /locus_tag="VNV63_02955"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HXC61611.1"
                     /translation="MQVIPAVDIKNGRCVRLRQGRMDDETVYAENPVEAAKRWEQAGA
                     KLLHVVDLDGAVGGVPKNKRVIQEIAAAVSIPLQVGGGIRNIKTIEDYLAAGIHRVIL
                     GTAAVLQRTLVTEACRRFPKCILAGIDAVDGRVAVDGWKTVTQEKATDLAIELEGIGL
                     AAIVYTDIKRDGMLSGPNLDAIRQLIACVSTPVIASGGVTTLEDLKSLKQLESLGLSG
                     AIIGKALYAGTLDLAEALSVAEGVS"
     gene            761..>1043
                     /locus_tag="VNV63_02960"
     CDS             761..>1043
                     /locus_tag="VNV63_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HXC61612.1"
                     /translation="MLAKRIIPCLDVKDGRVVKGVNFVGLKDAGDPVEIARRYDLEGA
                     DEICFLDITASHEERKILLDVVERTAEKVFIPLTVGGGIRTLDDIRDLLK"
BASE COUNT          253 a          235 c          336 g          219 t
ORIGIN      
        1 gaaaccctgc ccaagaaaaa gagatagaaa tgcaggtgat ccctgcagtc gatatcaaaa
       61 acgggcggtg cgtccgtctt cgccagggac ggatggacga cgagaccgtc tatgccgaga
      121 atccagtgga agccgcaaag agatgggagc aggcgggcgc gaaactgctt catgtcgtgg
      181 atctggatgg agcggtgggc ggtgttccta agaataaaag agtgattcaa gagatcgcag
      241 cagcggtttc tataccgttg caagtgggcg gaggaatacg gaacattaag accatcgaag
      301 actacctggc tgcaggaata catcgcgtta ttctcggtac ggcggcagtg ctgcagcgta
      361 ctttggtcac cgaggcctgt cgcagatttc cgaaatgcat cctggccggg attgatgcgg
      421 tggacggtcg ggttgcggtg gatggctgga agaccgtgac ccaagagaaa gcaacggatc
      481 tggcaataga gttggaaggg atcgggctcg cggcaatcgt ctataccgat atcaagcggg
      541 acgggatgct ctccggtcct aatctagatg ccatccggca gttaatcgcg tgtgtttcga
      601 ccccggtgat cgcttcggga ggagttacga ccctagagga tttaaaatcg ctcaagcaac
      661 tcgagtctct cggcctttcg ggcgcaatca tcggaaaggc actatatgcc gggaccctgg
      721 acctagcgga ggctctatcg gttgccgagg gagtctcctg atgttggcaa aacgaatcat
      781 cccgtgtctc gatgtcaaag acggtcgggt cgtcaagggc gtgaactttg tgggtctgaa
      841 agatgccggg gatccggtcg aaatcgcacg gcgctatgat ttggaagggg cggacgagat
      901 ctgttttttg gacattaccg cgtcgcatga agaacgaaag attttattgg atgtggtgga
      961 acgaaccgcg gaaaaggttt ttatcccgtt aaccgtggga ggcgggatca gaacgctcga
     1021 cgacatccgg gatctgttga aag
//
LOCUS       DATMKH010000246         1038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1398429, whole genome shotgun sequence.
ACCESSION   DATMKH010000246 DATMKH010000000
VERSION     DATMKH010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1398429"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="VNV63_02965"
     CDS             <1..476
                     /locus_tag="VNV63_02965"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXC61613.1"
                     /translation="FNTSESHRAIAEAIQQMWKEELNIVVTLENQEWKVYLDTQNNKN
                     YDLSRSAWIGDFMDPVTFLSMWTTGNGNNNTNWGSPKFDALIEQAARTGDPTARFKIL
                     RDAEDIFLTELPIVLVYWYTDTYLIRPSVQNWNPLVLGNHNYKFIDLKTERDPAK"
     gene            473..>1038
                     /locus_tag="VNV63_02970"
     CDS             473..>1038
                     /locus_tag="VNV63_02970"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HXC61614.1"
                     /translation="MIKIIVGRLLQLIPVLWMILTLTFFMSKEVPGGPFSAERQATPE
                     VIARLNAYYGLDRPLWKQYLGYLGKFARFDLGPSYTYQSESVNEVIARSFPISLQIGT
                     LAFCIASVLGITAGVIAAAHKNKVWDYFPMSLSMLGICLPTFVLGPILALLFGLVLRL
                     APVSGWHGLNYVPFHPMIMPAVTLGLFYS"
BASE COUNT          230 a          283 c          267 g          258 t
ORIGIN      
        1 atttcaacac ctcggaatca catcgagcga ttgccgaagc gatccagcag atgtggaagg
       61 aagagctgaa catagtcgtc accctggaga accaggaatg gaaggtctac ctggacaccc
      121 agaacaacaa aaactatgac ctctcacgtt ctgcatggat cggagatttc atggatccgg
      181 tcactttctt gagcatgtgg acgacaggca atggcaacaa taatacgaat tggggaagtc
      241 cgaagttcga tgcgctcatc gagcaagccg ccaggacagg agatccgacc gcccgcttca
      301 aaattctccg tgacgccgaa gacattttcc tcaccgaact tccaatcgtg ctggtttact
      361 ggtacaccga cacctacttg atacggccgt ccgtccagaa ttggaatccg ctcgtcctcg
      421 gtaaccacaa ctacaagttt atcgacctga agacggagcg cgatccggct aaatgatcaa
      481 gatcatcgtc ggacggttgc ttcagctcat cccggtgctc tggatgattc tgactctgac
      541 tttcttcatg tcgaaagagg tgccgggcgg tcctttcagc gcggaacgtc aggccacacc
      601 ggaagtcatt gccagattga atgcctacta cggcctcgat cgaccgctct ggaagcaata
      661 tctcggctat ttgggcaaat ttgcccgctt cgatctgggt ccctcctaca cgtatcaaag
      721 cgagtctgtc aacgaagtga ttgcgcgttc ttttccgatc tcgttgcaga tcggaacctt
      781 ggctttttgc atcgcttccg tgcttgggat caccgccgga gtaatcgcgg cagcgcacaa
      841 gaacaaggtg tgggactact ttccgatgtc gttgtcgatg ctggggatct gtcttccgac
      901 ttttgttttg ggtcccattc tagccttgct ctttggtctg gtgctgaggc tcgcgccggt
      961 cagtggttgg cacgggctga actatgtgcc atttcatccg atgatcatgc ccgccgtaac
     1021 ccttgggctg ttctattc
//
LOCUS       DATMKH010000247         2056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3951301, whole genome shotgun sequence.
ACCESSION   DATMKH010000247 DATMKH010000000
VERSION     DATMKH010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3951301"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(331..1707)
                     /gene="argH"
                     /locus_tag="VNV63_02975"
     CDS             complement(331..1707)
                     /gene="argH"
                     /locus_tag="VNV63_02975"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HXC61615.1"
                     /translation="MKPKSKSWGGRFTGGTHALAERFTASIQFDQRLARHDIEGSIAH
                     CLALTEAKVLTKEEAETMIQALKGIQQEISSGQFVFSIEQEDIHMAIEQRLTEKIGEL
                     GGKLQTARSRNDQVALDLRLFLREEIGEIRERMTYLQQTLVGLAKKHLDTVMPGYTHL
                     QPAQPVLFAHHLLAYYEMMDRDQARLADCLLRMNVLPLGSGALAGTNYPVDRQYLARL
                     LGFPKVTQNSLDAVSDRDFVVEFLSCVALIMMHLSRLGEELILWSSEAFGFLSLPDAF
                     CTGSSLMPQKKNPDILELIRAKTGRVYGHLVGLLTLLKGLPLAYNRDLQEDKEAVFDA
                     VDTTQACLEVAAELIKGIQVNKKRMEQAAQAGFLLATDLADYLVTKGLPFRQAHQTVG
                     RIVGYCLKKGKRLDQLSVSELKKFSNRFGKNVKGYLTVERSLARKNQIGSTARQQVME
                     RIKKIEAR"
     gene            complement(1704..>2056)
                     /locus_tag="VNV63_02980"
     CDS             complement(1704..>2056)
                     /locus_tag="VNV63_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003610143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HXC61616.1"
                     /translation="SITMDREVMHLRDSLIPRYAEIIYNGYWFSPEREMLQAAVDQAQ
                     IGVTGRVRLKLYKGSCQAIGRQAPDSLYRPELATFEEGEAHYRQHDAEGFIRLNALRL
                     KLKATQNKKGRRDA"
BASE COUNT          432 a          575 c          546 g          503 t
ORIGIN      
        1 ggggtccgcg acgcaataac cggtagagac agctttggaa accagatggc ctgcatctcc
       61 ttgggattga ttcgatacgg attgtcgtcc ggatagggat caatcaagag gagaagcctc
      121 cttcggtggg tggttattat actcgttggg atctcgatgg tttccatggt gcctccttat
      181 ccagctaact gcaatgactt catcttatta ggattgaaaa actattataa gagctttctt
      241 cctttttcgg ttgatcattc ggagcgattg gatcgccttt tcggccgcac gcggccatca
      301 gtaaaataag gaggagacag gcggtcagcc tcatctcgcc tcgatcttct tgattcgttc
      361 cataacctgc tgccgggccg tggagccgat ctgattttta cgggccagcg accgctctac
      421 ggttagatag cctttcacgt tttttccaaa gcggtttgaa aacttcttga gctcgctgac
      481 actgagctgg tccagccgtt tccctttttt aagacaataa ccgacgatcc tgcccacggt
      541 ctgatgagcc tggcgaaaag gcagcccctt ggtcactaga taatccgcca gatcggttgc
      601 gagcaggaaa ccggcctgcg ccgcttgttc catcctcttt ttgtttacct gaatgccttt
      661 gatcagctcc gccgcgacct ccaggcaggc ctgagtcgtg tccaccgcgt caaagaccgc
      721 ttctttatct tcctgtagat ctcgattata agccaaaggg agacccttta gcaatgtcag
      781 caagccaacg agatgaccgt agacccggcc ggtcttggcc cgaatgagtt cgaggatgtc
      841 cggatttttt ttctgcggca tgaggctgct tccggtgcaa aaagcatccg gcagcgaaag
      901 aaacccaaat gcctcggatg accacaggat aagctcttcg cccagccggg aaaggtgcat
      961 catgatcagc gcaacgcatg agaggaactc caccacgaaa tcccggtcgc ttaccgcatc
     1021 caggctgttt tgggttacct tgggaaatcc cagcagacgt gccagatact gacggtccac
     1081 cggataattc gttccggcaa gcgctccgga tcccaacggc aggacattca tgcgcagaag
     1141 acagtcagcg aggcgtgcct gatcgcggtc catcatctcg taataagcca ggagatggtg
     1201 agcaaacagg accggctggg ccggttgaag atgtgtgtag cccggcatca ccgtatccaa
     1261 atgcttcttg gcgaggccca ccagcgtctg ctgcaaatag gtcatccgtt cacggatctc
     1321 gccaatttcc tcacgaagga agagccggag gtctaatgcc acctggtcgt ttcggcttcg
     1381 tgcggtctgg agcttgcctc ccagttcacc gattttttcg gtcagtcgct gctcaatcgc
     1441 catgtggatg tcttcctgct cgatcgagaa cacaaactgg ccggatgaga tctcctgctg
     1501 aattcctttg agcgcctgga tcatcgtctc agcctcttcc ttcgtcagga ccttggcttc
     1561 ggtcaaggcc aggcagtggg cgatgcttcc ctcaatgtcg tgccgtgcga ggcgctgatc
     1621 aaactgaatc gaagcggtaa accgctcagc cagagcgtgg gtcccaccgg tgaagcgtcc
     1681 tccccaggat ttggattttg gcttcatgcg tcccggcgtc ccttcttgtt ttgtgttgct
     1741 tttaacttaa gtcgcaatgc attcagacgg ataaaaccct cggcatcgtg ctgccggtag
     1801 tgggcctcgc cctcttcgaa cgtggccagc tcgggccggt aaagagagtc cggagcctgg
     1861 cgtccgatcg cttgacagct gcccttgtat agttttaatc ggacccggcc tgtaacaccg
     1921 atctgggcct gatccaccgc ggcctggagc atctcacgct ccggtgaaaa ccaatagccg
     1981 ttgtagataa tctcggcata gcgcggaatc agtgagtccc gtagatgcat cacttcgcga
     2041 tccatcgtga tggatt
//
LOCUS       DATMKH010000248         1576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2040916, whole genome shotgun sequence.
ACCESSION   DATMKH010000248 DATMKH010000000
VERSION     DATMKH010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2040916"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1294
                     /gene="gatB"
                     /locus_tag="VNV63_02985"
     CDS             <1..1294
                     /gene="gatB"
                     /locus_tag="VNV63_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HXC61617.1"
                     /translation="PVLNKRVVDMGIMAGLATGCQIAPFSRFARKNYFYPDLPKGYQI
                     SQYELPLCEHGHLEIPVNGGTKRINIHRMHMEEDAGKNMHEGITAASHVDLNRAGVPL
                     LEIVSEPDIRSADEAVAYLKKLRDIIVYLGICDGNMEEGSFRCEPNISLRPVGSKEFG
                     TRVEVKNINSFKYVQAAMGHEIKRQTQVLNEGGRIIQETRLWDVEKEITVPMRSKEEA
                     HDYRYFPEPDLVPLNITKDRIESLRAQLPELPDAKRLRLVHDHGIPEYDAEVLTISRA
                     LADYFEACVREYPNPKIVSNWVMGDLLRELKNEGKEPDESPLKPKQLAAMLRLIDAGT
                     ISGKIAKDVFVEMYRTSKSPDAIVKEKNLVQVSDDAQIEKLIDQAFAASPNEVQKYRE
                     GKETLFGFFVGQVMKLSQGKANPGIVNELLKKKLGGSN"
     gene            1330..>1576
                     /gene="eno"
                     /locus_tag="VNV63_02990"
     CDS             1330..>1576
                     /gene="eno"
                     /locus_tag="VNV63_02990"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013150542.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HXC61618.1"
                     /translation="MSDIIDIFAREILDSRGNPTIEVEVILESGVISRAAVPSGASTG
                     LHEAVELRDKDKRRYGGKGVTRAAANVRDEIRPEITGC"
BASE COUNT          397 a          362 c          492 g          325 t
ORIGIN      
        1 gcccgttttg aataaaagag tcgtcgacat gggaatcatg gccggccttg cgaccggatg
       61 ccagatcgcc ccgttcagcc gctttgcgag aaagaattat ttttacccgg atctcccgaa
      121 ggggtatcag atctcgcagt acgagctgcc gctctgcgag catgggcatc tggagattcc
      181 cgtcaacggc ggcacgaaac gcatcaacat ccatcgaatg cacatggagg aggacgctgg
      241 caagaacatg catgaaggca ttaccgctgc aagccatgtg gacctgaacc gggccggtgt
      301 cccgcttttg gagatcgtaa gcgagccgga tatacgttcg gcggacgagg cggtcgctta
      361 tcttaaaaag ctccgcgaca tcatagtcta tctcggcatc tgcgatggaa acatggaaga
      421 gggaagtttc cggtgcgagc cgaatatctc tctgcgtccg gtgggatcaa aagagttcgg
      481 aacgcgggtg gaggtcaaga acataaactc gtttaaatat gtccaggcgg ccatggggca
      541 tgagatcaaa aggcaaacac aggtcttgaa cgaaggcggc cggattattc aggagacccg
      601 cctatgggat gtggagaaag agatcacggt tccgatgagg tccaaagagg aggcacacga
      661 ctatcgctat ttcccggagc cggatcttgt gcctttaaac atcacaaagg accgtatcga
      721 aagtctgcgc gctcaactcc cggagcttcc ggatgccaaa cggctccggc ttgttcacga
      781 ccacggcatt ccggagtatg atgccgaggt gcttaccatt tcccgggcgc tggcggatta
      841 ttttgaagcc tgcgtgcggg aatatccaaa cccgaagatc gtgagcaact gggtgatggg
      901 ggatcttctc cgcgagctga agaacgaggg gaaagaaccg gacgaaagtc cgctcaaacc
      961 taaacagctt gctgcaatgc tgaggcttat tgacgccggg acgatcagcg gcaagatcgc
     1021 aaaggatgtc tttgttgaga tgtaccggac cagcaagtcc ccggatgcca ttgtcaaaga
     1081 gaagaacctg gtgcaggtgt cggatgatgc gcagatcgag aagctgattg atcaggcgtt
     1141 tgctgcgagt ccgaatgagg tacagaagta ccgggagggg aaggagacgc tctttggctt
     1201 ttttgtaggc caggtgatga aactgtcgca ggggaaggcg aatcccggca ttgtgaatga
     1261 gttgttaaaa aagaagctgg gaggttcaaa ctgaaaaatg tcccttttgc tcttaataat
     1321 tggtcgatca tgagcgatat tatagatatt tttgcaagag agattttaga ttcgagaggg
     1381 aatccgacga tcgaagtgga ggtgatactg gagagcggtg tgataagccg cgccgcggtt
     1441 ccgtccggtg cctcgaccgg tctgcatgaa gcggtggagc tgcgggacaa ggacaagcgg
     1501 cgctacggag gcaagggcgt cactcgggct gcggccaatg taagggatga gatccgcccg
     1561 gagattacgg ggtgtg
//
LOCUS       DATMKH010000249         1055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3324596, whole genome shotgun sequence.
ACCESSION   DATMKH010000249 DATMKH010000000
VERSION     DATMKH010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3324596"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VNV63_02995"
     CDS             <1..219
                     /locus_tag="VNV63_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61619.1"
                     /translation="CKVGGHPVLERYVTVSIVYNVEDGYWHCLICGFIGFGSNYELLQ
                     YRLVACLGGSPRLPGKKTRAKKKLNKET"
     gene            231..>1055
                     /locus_tag="VNV63_03000"
     CDS             231..>1055
                     /locus_tag="VNV63_03000"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HXC61620.1"
                     /translation="MRRYVEWMIRYRFLVMGLTLLATGALAFQIKNLKIVIDPNTNLP
                     QDHPYVMATNQVEKLFGSKNVIVIGITPKPGNAYQPDVLAKVQRITTALLDTPDILKE
                     NTLSLSARRAKNISGTADGMEVRPLMETVPQTPQAIEALRQAVHNNPVYSNTIVSKDE
                     RTAAIIAEYRDPQNGYQGIMEKVQSIVNRERDNTVEIAVGGWPVYGAQLERYSQRMAF
                     LFPLAVLITGLIHYEAFRTIQGLILPLVTALLAVVWGVGIMGASGVPMDAFNASTPI"
BASE COUNT          254 a          270 c          294 g          237 t
ORIGIN      
        1 tgtaaggtgg gcggacatcc tgtcctggaa cgttatgtga cggtgtccat tgtgtataat
       61 gttgaggatg gctactggca ctgcctcatt tgcgggttta ttggttttgg tagcaattac
      121 gagctgctcc aataccgcct agtggcctgt ctgggtggat ctccccgcct tcccgggaag
      181 aagactcggg cgaaaaaaaa actgaataaa gagacatagg atcctagttc atgcgacgtt
      241 atgtagaatg gatgatccgt tacaggtttc tcgtcatggg actcactctg ctcgcgacag
      301 gggcgctggc tttccagatt aagaacttaa agatcgtgat agatccgaat accaaccttc
      361 ctcaggatca cccctatgtc atggcgacaa accaggttga aaagttattc ggatcgaaaa
      421 acgtgattgt gatcgggatt acaccgaagc caggaaacgc ctatcagccg gatgtgctcg
      481 cgaaagtcca acggatcacc accgctcttc ttgatacacc ggacatactg aaagaaaata
      541 cactcagtct gtcagcacgc cgggccaaga atatctctgg aacggcggat gggatggagg
      601 tccgcccttt aatggaaacg gtaccgcaga caccgcaagc gatcgaggcg ctgcgtcagg
      661 ccgtccataa caatcctgtc tattccaata ccattgtctc caaagatgag cgaacggctg
      721 cgattatcgc ggagtacaga gatccccaaa acgggtatca ggggatcatg gaaaaagttc
      781 agtccattgt gaatcgggaa cgtgacaata cggtcgagat cgccgtagga ggatggccgg
      841 tctacggggc acagcttgag cgctactcac agcggatggc gttcctcttc ccgcttgcgg
      901 tcctcatcac cggactgatc cattatgagg cattccggac gattcagggg ctgatcctcc
      961 cgctggtcac cgccctgctc gcggtcgtct ggggtgtggg gatcatgggg gcgtccggtg
     1021 tcccaatgga cgccttcaat gcgagcacgc cgatc
//
LOCUS       DATMKH010000250         1604 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2041097, whole genome shotgun sequence.
ACCESSION   DATMKH010000250 DATMKH010000000
VERSION     DATMKH010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2041097"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="VNV63_03005"
     CDS             complement(<1..278)
                     /locus_tag="VNV63_03005"
                     /inference="COORDINATES: protein motif:HMM:NF025918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HXC61621.1"
                     /translation="MTQEDFDNLINEIREEDPDQLSSEFLVREDPIKEEGIVKFEQKA
                     GFKFPEEYRYFVKKYGAGEIGTITVLSPDPESQFSMWDGKEEFNRETG"
     gene            515..961
                     /gene="cynS"
                     /locus_tag="VNV63_03010"
     CDS             515..961
                     /gene="cynS"
                     /locus_tag="VNV63_03010"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HXC61622.1"
                     /translation="MTSPSAAIELIREAKGRKGLTWEQIAAHLGRSPVYITSACLGEN
                     SLSSEDAKKLGALLDLAPDIVALLQSCPKKGSGSLDIPKDPLLYRFHEINLVYGDTLK
                     ELIHEKFGDGIMSAIDFTMEVEKVEDPKGDRVKVTMCGKFLPYKKW"
BASE COUNT          403 a          437 c          380 g          384 t
ORIGIN      
        1 cagcctgttt ctcgattgaa ctcttccttc ccgtcccaca tggagaactg actctccggg
       61 tccggagata aaaccgtaat cgtgccgatc tctccggcac catatttctt aacaaaatac
      121 cgatattcct ctgggaactt aaaccctgcc ttctgctcaa atttcacgat cccttcctcc
      181 ttgatcggat cttcccgcac caagaactcg gagcttagct ggtctgggtc ttcttctcgg
      241 atttcgttaa ttaagttgtc gaaatcttcc tgagtcatag ccgatgaaag ccatacattg
      301 cacctttctt aaccgcagca agtgcttaaa cgcgtcctag cccttaaatg acaaattaca
      361 aacgacaaat gacagattgt cttgggctta cgcgagccga ccagggacaa tttgtaattt
      421 gtcatctcta atttgtcatt ccgttccggt cgctgtcctg gcacgaacct cgctccggag
      481 aaccgtgcca tttcgaacca gcaatcaaga ctctatgacc tctccatccg cagccatcga
      541 actgatccgg gaagccaagg gaagaaaagg tctgacctgg gagcaaatcg cagcacacct
      601 cgggcgctcc ccagtgtata taacctcagc ctgcctgggc gaaaatagcc tgagctcaga
      661 agacgccaaa aagctcggcg cacttctcga cctcgctcca gacatcgttg cgttactaca
      721 gtcctgtccc aaaaaagggt cgggatcgct agacatcccg aaagaccccc tcctataccg
      781 gtttcacgag atcaacctgg tctatggaga tacgctcaag gagttgattc acgagaaatt
      841 cggggacggg atcatgagcg ccatcgattt caccatggaa gtcgagaaag tagaagatcc
      901 caagggagac cgggtaaagg tcaccatgtg cggcaagttt ttgccctata agaagtggta
      961 gcacagagcg gcctggaggg gagccgcttt ggaacgtgcc taaaagatct agtgatattc
     1021 ctcccagtgc gaggaaggcc gcgtatagcg ttaaacatac ggctcccggg aacgtttcaa
     1081 cgcgtgttgg gtctccggcc cgctacttga atgaataatc tcccaacgga accatgggca
     1141 aaacatcgtg tgcggtaatt cgcgggagcc gcctgtttaa caccattttt tgccttcctc
     1201 gcaccggaga gaaattacga aatcgttgag tacgttctaa agcggctccc ctccattcgc
     1261 cgtttcacca atcacatctc ccctgtccca aaagagccac ccctgaccaa accagaaacc
     1321 ccggttggtc ttttcttttc attacattta tggagaacat gggatttaat cacttctctt
     1381 cggatcatgt catcggattg ccttggcata gcagctgcta acctaaatat cggcttcatt
     1441 cggggggagg tccgaatgta tatcgcgcta agggataggc cgagtttgaa acgacacagg
     1501 gaagagaggg gtcaataaac tctgagccaa gggtgcgtga agtctctggc ggggaagtgg
     1561 acttggaggg agtaccactt ccctgctcct ttttagacgc gatt
//
LOCUS       DATMKH010000251         2109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_119090,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000251 DATMKH010000000
VERSION     DATMKH010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119090"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="VNV63_03015"
     CDS             complement(<1..533)
                     /locus_tag="VNV63_03015"
                     /inference="COORDINATES: protein motif:HMM:NF025692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HXC61623.1"
                     /translation="MAEGVGPVTGRVNARIFSPRILFWLGFFVLLQFFWFGVTEGWTA
                     DERVSDLTVKTAPQGEILVSASLLQPMQGKAANDIQHGIPKDLFYYVLLMKRQPAWFD
                     EELLAKTIKYTIQYDVLKKQYRVHVRHETDAANSIVENFQEMVDLISKIDNVKIADVK
                     MLKPKETYYVRVKAQMKA"
     gene            complement(509..2035)
                     /locus_tag="VNV63_03020"
     CDS             complement(509..2035)
                     /locus_tag="VNV63_03020"
                     /inference="COORDINATES: protein motif:HMM:NF024876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXC61624.1"
                     /translation="MTRPSNKEEKITGMREAAGLKISLALVAIVIGLIGLIAYQLFRI
                     PIPADREHAGEARSATSDRSSTLNEPPSRTTGGSFTRRDLVVELNLIKPSSDLPPLRL
                     SEGDDRSSGGRQAGMEENQAAVEQFQAGRYEAAAALLGKAHELDPTNPVFTKNLAYAK
                     ARIAWNQVDQAEYETALTGFQSAITLQSQEFSFYFGLGVSYHRLKKEAQAKEAAEKAL
                     RLAPNESAPYKLLGEIAYQNDQLDEALGYFEKALQLVPRDPNLPSVIEKLQREHQVQS
                     QFQQEATIHFTVKFEGHEERDVADQVVRILEEAYGDIGKSFSYYPESPITVILYSDQQ
                     FRDITRTPAWTGGLFDGKIRIPTEGAGFSPEVLNRVLHHEYTHAIVYALTDGHVPTWL
                     NEGLALNFEKGDPASSSSVSSSAWDSPIQGAIRTNSLISLQALHGSFMNMDEATSRLS
                     YAESYSAVKYLIDRYGMFAIQSLLKDLSRQRDFDKAFEDRFFISYQEFQTAWQKGLAP
                     "
BASE COUNT          458 a          547 c          575 g          529 t
ORIGIN      
        1 gccttcatct gcgctttgac cctgacatag taggtctctt tgggcttgag catctttacg
       61 tcggctattt taacattatc aatttttgag atcaaatcca ccatctcctg aaaattttca
      121 acgatactgt tggccgcatc cgtctcatga cggacgtgaa cacggtactg tttttttaag
      181 acatcatact ggatcgtgta cttgatcgtc ttggcgagca actcttcatc gaaccaggca
      241 ggctgccgtt tcatcagcaa aacgtaatag aagagatctt tgggtatccc atgctggatg
      301 tcgtttgccg ctttgccctg catgggctgg agaaggctgg ccgagactaa gatctcgcct
      361 tgtggagcgg tcttgacggt caaatcggaa acccgttcat ctgccgtcca tccctctgtg
      421 acgccgaacc agaaaaactg aagcaggacg aagaagccga gccagaagag gattcttggg
      481 gagaatattc gagcattcac gcggccagtc acggggccaa ccccttctgc catgcggttt
      541 gaaattcctg gtaggagatg aagaatcgat cctcgaaggc cttgtcaaaa tcacgctgac
      601 gggagagatc cttgagcagc gactgaatag caaacattcc gtatcgatct atgaggtact
      661 tcacggcaga gtagctttcg gcataggaaa gccgtgaggt ggcctcatcc atgttcataa
      721 aagaaccgtg aagtgcctgg agtgaaataa ggctgttggt gcggatcgct ccctgtatgg
      781 gagagtccca agcggatgag gatacagaag acgaggacgc cgggtcccct ttctcgaagt
      841 ttagggcgag cccctcattc agccaggtgg ggacatgacc gtctgtcaag gcataaacga
      901 tggcatgcgt gtattcatga tgcagaacgc ggtttaagac ctccggcgag aatccggctc
      961 cttcggttgg gatccggatc tttccgtcaa agagcccgcc ggtccaagcg ggtgtccgag
     1021 tgatgtcgcg gaactgctgg tcggaataaa gaatcaccgt gatcgggctc tccggatagt
     1081 aagagaaact ctttccgata tcaccgtagg cctcttcgag tatccgtacc acttgatccg
     1141 cgacatcccg ttcttcatgt ccttcaaatt tgaccgtgaa atggatcgtt gcttcttgct
     1201 ggaactggct ctggacctgg tgttcccgtt ggagtttttc aatgactgat ggaagattgg
     1261 gatcacgcgg cacgagctga agcgcctttt caaaatagcc cagcgcctca tcgagttgat
     1321 cgttctggta ggcaatctca cccaggagtt tgtagggagc cgactcgttc ggggcaaggc
     1381 gtaacgcctt ctcggcggcc tctttggcct gggcctcttt tttcagacgg tgataactca
     1441 cgcctaagcc gaagtagaat gaaaattcct gggattgaag tgtaatcgcg ctctggaaac
     1501 cggttaaggc ggtttcatac tccgcctgat caacttgatt ccaggcgatc cgcgccttgg
     1561 cataggccag gttcttcgtg aagaccgggt ttgtcgggtc cagttcatgg gctttcccga
     1621 gcagcgctgc ggcggcctca tatcgccccg cctggaactg ttctactgct gcctgatttt
     1681 cctccatccc ggcctgtctg ccccctgatg atcggtcatc tccctcgctg agtcttaagg
     1741 gcgggaggtc cgaagaaggc ttgatgagat tcagttcaac cactaagtct cgacgagtaa
     1801 aagacccgcc tgttgttcgg ctgggaggtt cgtttaaggt ggaagaccga tccgaagtgg
     1861 cggatcgggc ctctccggca tgctcccgat ccgcgggaat cggaatccga aagagctggt
     1921 aggcgataag gccgatcagt cctatcacga ttgccactaa cgcaagggag atcttcagcc
     1981 ctgcggcttc ccgcatcccg gtgatcttct cttctttatt ggatgggcga gtcactcttt
     2041 tatctctcct caatctgata cacgagtctg atcccgcgct tgtcttcgga gagcgttgac
     2101 ttgaggtct
//
LOCUS       DATMKH010000252         4149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4595880, whole genome shotgun sequence.
ACCESSION   DATMKH010000252 DATMKH010000000
VERSION     DATMKH010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4149
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4595880"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            93..677
                     /locus_tag="VNV63_03025"
     CDS             93..677
                     /locus_tag="VNV63_03025"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HXC61625.1"
                     /translation="MGTLHPNFIVGIGGSAGGLDAYTVLLDALPSNTGMAFVIISHIL
                     PTTAHSMLAELLTSHTKMPVIVVSDAMPIEADHIYVIPPDADLFIEGNTFKAVSPRTM
                     NKQIDIFLISLAAAMGARAIGVIVSGYDGDGSEGCKQIKAKGGKTFAQDTSAKVPGMP
                     ESARASGSVDFVLPPDKIAAELQRLASASINKRS"
     gene            1164..>4149
                     /locus_tag="VNV63_03030"
     CDS             1164..>4149
                     /locus_tag="VNV63_03030"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HXC61626.1"
                     /translation="MKKTVKKTRTRTKPPVSEPSKSRVSSSKVAKTVVKKSEENDFCV
                     AGIGASAGGLEALEEFFRHTPIDTGLAFVVVQHLDPSHKSIMVELLQRCTKMQVHQVE
                     DGMLIRPNFVYVIPPNKQLAVLHGKLHLIDLDPLRPIRLPIDFFLCSLADEKGNKAIC
                     IILSGTGTDGALGLRAVKVAGGMTMVQEESTAKFDSMPRNAIATGSVDRILPAAKMPS
                     ELIKYACHPYTRMAMEEMEEPKNTGDMDKIFILLRTHTGHDFSNYKRTTTGRRIERRM
                     AVHQINDIKQYLHYMQQNKTEIDALFKELLIGVTSFFRDREAFEVLEKKVIPRLFENK
                     TPDIPIRVWVAGCSTGEEAYSIAILLREAMEKLPKTFKAQVFATDIDQQALEVARHAY
                     YPESIATNIPPAHLNFFSKEGTNYRVKQAVREMMLFTKHDLLRDPPFSKLDLVVCRNL
                     LIYLSPALQKKVLPLFHYTLNPKGFMMLGPSETIAEFVDLFELVDKKWKVFRRKQLIG
                     HVAELPMAPLARMGIDQRKRSPKVSTDLNTIQRIEKILLEEYTLPCAVITEKNEILYI
                     RGRTDRYLEPAVGEASMNILKMAREGLRIPLRTALQKALRDRTTVFYPKVRVQGNRMH
                     YTVNLMIKPFDKKSMPGLVLVVFEDVTSLSPPGIKREKTKRVFRADQRAVELEQELNS
                     AKATLQTMVDELELTNEELKSSNEELQSTNEELQSTNEEQETSKEELQSVNEELTTVN
                     SELQSKMDELSQNNSDVVNLLKGTEIATFFLDNQLQIKRFTPTVARLFNLIPGDIGRP
                     ISHIAPNLVGQDIALDAKEVLKTLVSKEREVRTKTGSWFLMRIMPYRTLANVIDGVVA
                     TFVDITDLKKQKLAAEAALRYAEAVIATIREPLIVLDGNLRVVSANPAYRRTFEVKKE
                     ETEGVLIYDLGDGQWNIPKLKSLLEEILPQNTSIENFEVEHTFKGIGHKKMSLNARKI
                     VTPEEETQLILLAIRDITLEGKR"
BASE COUNT         1087 a         1028 c         1107 g          927 t
ORIGIN      
        1 agagagagag agagagagag agagagagag agagagacaa agcaccaaag gtacaaacga
       61 tcggatagaa acctggagac ctaatgtaag ccatgggaac attacatcct aacttcattg
      121 tcggcattgg cggttcagcc ggaggtcttg acgcctatac ggtgcttttg gatgccctgc
      181 cgtctaatac aggtatggcc tttgtcatta tttctcatat tctccccacc accgcccata
      241 gtatgttagc cgaacttctg acgagtcaca cgaaaatgcc ggtcatagtg gtttccgatg
      301 cgatgccgat cgaggcagac cacatctacg tgatccctcc ggacgcagat ctttttattg
      361 agggtaatac cttcaaagcc gtctccccgc gcactatgaa caagcagata gatatatttt
      421 tgatctcttt agcagcggcg atgggcgcgc gcgcgatcgg cgttatcgtc tccgggtatg
      481 atggcgacgg tagtgaggga tgcaagcaaa tcaaggcaaa aggaggaaaa acctttgctc
      541 aggacacgtc tgccaaagta cccggcatgc ctgagagtgc tcgggcttca ggctctgttg
      601 atttcgttct gccacccgac aaaattgcag ctgaactaca aagattggca agcgcttcta
      661 tcaataaaag gtcgtgattg ccaagccagc caaaacttca aatgacccga tcgagctgct
      721 gcgggacctt gcgcgagcac actataattc ttcaaaactg agcaatattg accagaccgc
      781 aatctgtttt attaggtata acatatcgat ggatcgcatc attccaaggt tctccagcgg
      841 cacggcccgg caaaagaagg ctgagggtcg ttccggtctc ttaaagattc cttaagcgcg
      901 cttgaacttt ttggcacagt ggagtcggaa actgccgtga ttgatagtga tgaagaagaa
      961 agcagcgtgt ttgacttttt cctctaaagt ggttaatatt agaagcgtct attactagag
     1021 gcgttttcgg aatttagtcc gctgtttgct tcccgactta ccggccgcgg tgttcattcc
     1081 ttcgtacgac gccttattct ccgaaccttc cttgatacgg tcagcgtgga gggagatcta
     1141 cggtgaagaa agatctggca aaaatgaaga agacggtaaa aaagacacga acaagaacga
     1201 agcctccggt ttctgaacca tcgaaatccc gcgtttcttc ttccaaggtt gcgaagacgg
     1261 tcgtcaaaaa atcggaggaa aacgactttt gtgtggccgg catcggtgcc tccgcgggcg
     1321 ggctggaggc gctggaagaa ttttttcgtc atacaccgat cgataccggc ctggcgttcg
     1381 tggttgtcca gcacctcgac ccaagccata aaagcatcat ggtggagctg ctccagcgct
     1441 gcaccaaaat gcaggtccat caggtagaag acgggatgct tatccggccc aacttcgtct
     1501 atgtcattcc tcccaacaag caattagcgg tgttacacgg taagctccat ttgatcgatc
     1561 tggaccccct gcgtcccatt cggttgccga tcgacttctt tttgtgttcg ctggcggacg
     1621 agaaggggaa caaggccatc tgtattattc tgtccgggac cgggacggat ggggccctgg
     1681 gccttagggc ggtcaaggtc gccgggggaa tgacgatggt tcaggaggag agcacggcga
     1741 agttcgacag tatgccccgc aatgcgatcg ccaccggctc cgtggaccgt atactaccgg
     1801 ccgcaaagat gccaagcgag ctgatcaagt acgcctgcca cccttacacc cgcatggcca
     1861 tggaggagat ggaggagccg aagaatacag gcgacatgga caagatcttt atccttttgc
     1921 gcacccatac cggccatgat ttttccaatt ataaacgcac caccaccggc cggcggatcg
     1981 agcggcggat ggcggtccac cagatcaacg atatcaagca atatctccat tatatgcagc
     2041 aaaataaaac ggagattgat gccctcttta aagaattact gatcggggta accagtttct
     2101 tccgtgaccg agaggccttt gaggttctgg aaaaaaaggt gatcccccgc ctctttgaga
     2161 acaagactcc cgacatcccc atacgggtct gggtggccgg gtgttcgaca ggcgaggagg
     2221 cctattcgat tgcgattctc ttgagagagg cgatggaaaa gttacccaag acattcaagg
     2281 cgcaggtctt tgccaccgat attgatcagc aggcactgga agtggcccgt cacgcctact
     2341 atcctgaatc gatcgccacg aatatccctc ccgcacacct gaactttttt tcaaaagagg
     2401 gcaccaatta cagagttaaa caagccgtac gggagatgat gctgtttacg aaacatgacc
     2461 ttctgcgaga ccctcccttt tcgaagctgg acctggtcgt atgtcgcaat cttctgatct
     2521 atctgagccc ggcgctgcag aaaaaagtac tacccctgtt tcattacaca ctcaatccca
     2581 aggggtttat gatgctgggc ccttctgaaa caattgcgga atttgtggat ctctttgaac
     2641 tggtcgataa aaaatggaag gtattccgcc gcaaacagct aatcggccac gtggcggagc
     2701 ttccaatggc gccgctcgcc agaatgggga tcgaccaaag aaagagatcg ccgaaggttt
     2761 ctacagatct caatacaatt cagcgcatcg aaaagatact gctggaggaa tatacgctgc
     2821 cctgcgcggt tatcactgaa aaaaatgaga ttctctatat tcgaggccga accgatcggt
     2881 atctggagcc cgctgtgggg gaggcgtcca tgaatattct gaaaatggct cgtgaagggc
     2941 tgcgtatccc gctccggacg gcgcttcaaa aggccttgag agatcggacg accgtcttct
     3001 atcccaaggt ccgtgtccag ggaaaccgta tgcactatac cgtgaatcta atgatcaagc
     3061 cgttcgataa aaaatcgatg ccgggattgg tgctggtggt ctttgaggat gtcacgtccc
     3121 tgagcccccc ggggatcaag agagaaaaaa caaagcgtgt cttcagggcg gatcaacgtg
     3181 ctgttgagtt ggaacaggag ctcaattcgg cgaaggcgac gctccaaacg atggtagatg
     3241 agctggagct cacgaatgaa gagctcaaat cgtccaatga ggagcttcag tctaccaatg
     3301 aggaattgca aagtacgaat gaggaacaag aaacatccaa ggaagaactg cagtcggtca
     3361 atgaggagtt gacgaccgtg aactcggagc tccaaagcaa gatggatgag ctgtcacaga
     3421 acaatagcga tgtggtcaac ctgctcaaag gcaccgagat cgcgaccttt tttctggaca
     3481 accagcttca gatcaagcga ttcacgccga cggtcgcccg gctgtttaac ttgatccctg
     3541 gggatatcgg ccggccgatc agccatatcg cccctaatct tgtaggccag gatatcgccc
     3601 tggacgctaa ggaggtctta aagacgcttg tctccaagga aagggaggtt cggacaaaaa
     3661 cgggcagctg gtttttaatg cggattatgc cctatcgtac gctcgccaat gtgatcgatg
     3721 gggtggtggc gacctttgtg gacatcacgg atctgaaaaa gcagaagctg gccgccgagg
     3781 ctgctctccg atacgccgaa gcggtcatcg ccaccattcg agaaccgttg attgtgttgg
     3841 atggcaatct tcgggtcgtc tcggccaatc ccgcctatcg ccggacattt gaggtgaaaa
     3901 aggaagagac ggagggtgtg ctcatttatg acttagggga cggccaatgg aacatcccga
     3961 aattgaaaag tcttctggaa gagatccttc cccagaacac ctcgatcgag aatttcgagg
     4021 tggaacatac ctttaagggg attggtcata aaaagatgag tctcaatgcc cgcaagatcg
     4081 ttaccccgga ggaagaaacc cagttgatcc tattggccat ccgagatatt acgctcgaag
     4141 gaaaacgag
//
LOCUS       DATMKH010000253         1030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1082398, whole genome shotgun sequence.
ACCESSION   DATMKH010000253 DATMKH010000000
VERSION     DATMKH010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1082398"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="VNV63_03035"
     CDS             <1..400
                     /locus_tag="VNV63_03035"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the formation of
                     (E)-3-(methoxycarbonyl)pent-2-enedioate and
                     S-adenosyl-L-homocysteine from S-adenosyl-L-methionine and
                     trans-aconitate; Derived by automated computational
                     analysis using gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HXC61627.1"
                     /translation="GVLAFQVPYRKQQPVAEVYLRLINSDRWRHYPPPDERLTVEDPA
                     FYYDVLAGCCKEAEVWETVYYHSLENHRAMVAWYQSTGLRPFLANLPDDETRTEFMEE
                     LAGLYEKQFPPQIDGRVLFPFHRLFAVAVR"
     gene            547..777
                     /locus_tag="VNV63_03040"
     CDS             547..777
                     /locus_tag="VNV63_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61628.1"
                     /translation="MKTQDEAAVVSVRDNPDDGMARINLRWEGKHQISDFDLDKLGRV
                     LDSEDETEHSGWVFVERPVKIEAGKIIPMLKR"
     gene            complement(811..>1030)
                     /locus_tag="VNV63_03045"
     CDS             complement(811..>1030)
                     /locus_tag="VNV63_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015201869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fused N-dimethylarginine
                     dimethylaminohydrolase/saccharopine dehydrogenase
                     domain-containing protein"
                     /protein_id="HXC61629.1"
                     /translation="ILMLSSMLHSIGVGNMTPAGVKLVCVDINPAVVTKLADRGSIES
                     VGIVTDVGLFLSLLVQQVRAQSASKSAA"
BASE COUNT          250 a          268 c          276 g          236 t
ORIGIN      
        1 aggagtcctg gcttttcaag ttccttaccg gaagcagcaa ccggtagcgg aggtctatct
       61 gcgcctgatc aactcggata gatggcgtca ttatcccccg cctgacgaac gtttgacggt
      121 cgaagatcct gccttttact atgatgtcct ggctggttgc tgcaaggagg ctgaagtctg
      181 ggaaaccgtc tactatcact ctctcgaaaa ccatcgtgcg atggtagcct ggtatcagag
      241 tacggggctt cgaccctttc tggccaacct gccggacgac gaaactcgta cagagttcat
      301 ggaggagctg gccgggctgt atgaaaagca attcccgccg caaatcgatg gtcgcgttct
      361 ctttccgttt caccgcctgt ttgcagtcgc ggttcgctga tgaatccgtt cctcatgatc
      421 tgagcttctt catctgcggc ggaacgacct tttctccaga tgttcccggc tttccctcag
      481 ctccgaccga ccggccagga acgaatagag tttgtcaaga ttcatggaaa atagaaaaat
      541 atggatatga aaacccaaga tgaagcagcg gtcgtgagcg ttcgtgacaa cccggacgat
      601 ggcatggcaa ggattaatct gcgctgggaa ggcaagcacc aaatatccga tttcgacctc
      661 gacaagttag gcagggtcct ggattccgaa gacgaaacgg aacattccgg atgggttttt
      721 gttgaacggc ctgtaaagat cgaggcggga aaaattatcc cgatgctcaa aagataggat
      781 aatcgcatca cccggcggat aacattcgga tcacgcggct gactttgatg cgctttgtgc
      841 acgaacctgc tggacgagca ggctcaggaa gaggcccacg tccgtgacga ttccaacgct
      901 ttcgatcgag ccgcggtcag cgagtttcgt caccaccgca gggttgatat ctacacaaac
      961 aagcttaaca cccgcgggag tcatgttacc cactccgatg ctatgaagca ttgagctgag
     1021 catgagaatc
//
LOCUS       DATMKH010000254         1269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4287779, whole genome shotgun sequence.
ACCESSION   DATMKH010000254 DATMKH010000000
VERSION     DATMKH010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4287779"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /locus_tag="VNV63_03050"
     CDS             <1..760
                     /locus_tag="VNV63_03050"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HXC61630.1"
                     /translation="ETVRAYDGKIFRLEDHFDRLFRSATGIRLSIPHTREEFREILDQ
                     ILKKNKLKDALIRITITRGEGEVGSVLPPSVEPTVVILPRPFAGYPPASYEKGVKIVT
                     IKDQHHFPSGLHPEIKSTSFQKNILVKMQAQQKGADDAVLLNGHGYVMEGTSSNLFIV
                     KEGKIITPSPEVGILEGITRRTVMELAGGRGIPVHEGLLSREELYQADECFLTNTSVE
                     IMPVVVIDENSLGAGHPGPLTIFLMKAFREAVKE"
     gene            complement(768..>1269)
                     /locus_tag="VNV63_03055"
     CDS             complement(768..>1269)
                     /locus_tag="VNV63_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HXC61631.1"
                     /translation="KKQPIVLKDLLHPAYFVPETMKVSRLLKELQRRRMQMAIVVNEY
                     GSIEGLVTMEDLIEEIVGEINDESDIEERPIERLKDGSLVIDASLSVRDLKEDYFLPL
                     PESSEYETLGGFVLSHLQTLPRGGEIIQHGDYKFTIVDTEGRRIAKVKVEKVSLPAEP
                     TPSPKS"
BASE COUNT          304 a          321 c          321 g          323 t
ORIGIN      
        1 tgagaccgtt cgtgcttatg atgggaagat ctttaggctg gaagatcatt tcgaccgact
       61 ttttagatca gcgaccggca tccgattatc cattcctcat acgcgagaag aattcagaga
      121 gatcctcgat cagatcctca aaaaaaataa gctgaaggat gccctaatcc ggataacgat
      181 cacacgaggt gaaggcgaag ttggttcggt tctgccccct tctgtcgaac ccaccgtggt
      241 aatcttgccg cgtccgtttg ccggttatcc tcccgcgtcc tatgagaagg gcgtcaagat
      301 tgtaacgatc aaggatcagc atcattttcc ctccggtctt catcctgaaa ttaagtcgac
      361 cagctttcaa aaaaatattc tggtcaaaat gcaggcgcaa caaaaaggcg ccgatgacgc
      421 agtccttctc aacgggcatg gttatgtgat ggagggaacg tccagcaatc tttttattgt
      481 gaaagaagga aaaatcatca caccctcacc tgaagtcgga attttggaag gaattacccg
      541 tcgaactgtc atggagttag ccggaggccg gggcatcccg gttcacgagg gtcttctctc
      601 gcgtgaagag ctgtatcagg cggatgaatg ctttctgacc aataccagcg tcgaaatcat
      661 gccggtcgtg gttatcgatg agaattccct gggcgccgga catcccggac ccttgacgat
      721 tttcctgatg aaggcgttcc gagaagctgt aaaggaatga tccgtgacta gctttttggc
      781 gagggagtcg gttctgcagg cagcgagacc ttttcgacct tgaccttggc gatgcgtcgg
      841 ccttccgtgt ctacaatggt gaacttgtaa tcgccatgct ggatgatctc tccgccccga
      901 ggcaaggttt ggaggtggga caggacaaaa ccgcccaaag tttcatattc agacgactcc
      961 gggagcggaa gaaaatagtc ctccttgagg tctctcaccg aaagagacgc atcgatcacc
     1021 agtgagccgt ccttcagtct ttcgatcggg cgctcttcga tatcagactc atcgttgatt
     1081 tctcccacga tctcttcgat taagtcttcc atcgtgacca gaccttctat gctcccatat
     1141 tcattcacta caatggccat ctgcatgcgt cttcgctgaa gctctttgag tagacggctc
     1201 accttcattg tttctggcac aaagtaagcg gggtgaagca ggtctttgag cacgatgggc
     1261 tgcttcttg
//
LOCUS       DATMKH010000255         1089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_442194,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000255 DATMKH010000000
VERSION     DATMKH010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442194"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..636
                     /locus_tag="VNV63_03060"
     CDS             118..636
                     /locus_tag="VNV63_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61632.1"
                     /translation="MYSEDDLQYAIENTRVILSPERRIETFGTTSFHFYLVTELMDSV
                     SEIRVRDGRIQADRPQIITPANLTKLSLEGFGEKARVFAEMLQNRNSALLKYGFQIRK
                     DDLSESLVHDSVEAVLDRVTSQVPAALRSSSAVIHGVDEGWEVCLLKFTIEMMHQSAG
                     GNIQDFRRRGML"
     gene            695..>1089
                     /locus_tag="VNV63_03065"
     CDS             695..>1089
                     /locus_tag="VNV63_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61633.1"
                     /translation="MNNLVKVLIIAGLAIGIFGGGGYLAYRLFFKKPDRLKLGVNHAV
                     VTPTPDAGVTMFEQANHEIEHGDKESGEKILWALIQNLPDSEKNSDAKKLLSSLNVQE
                     FFSAQPGPDKTEYVVARGDSMARIAGKTK"
BASE COUNT          265 a          252 c          317 g          255 t
ORIGIN      
        1 tctttttctg agtccggcga cctctcgata aatccggaat gagatccgtc cagagccggt
       61 tgaacccctc cccgtttgag tccctcttca aatagggcta agctgaggga actctctatg
      121 tattcagagg acgatcttca atacgccatt gagaatacca gggtgatcct gtcgccggag
      181 cgcaggatcg agacttttgg tacgactagc tttcattttt atctggttac tgaactcatg
      241 gatagcgtta gtgagatacg tgtccgcgac gggagaatac aggcagatcg cccgcaaatt
      301 atcactccgg caaatcttac gaaattgtct ctggaagggt tcggcgagaa agcgcgagta
      361 tttgccgaaa tgctgcaaaa tcgaaattcc gcgctgttga aatacggctt tcaaatccgg
      421 aaggacgacc tttctgagag cctggtccac gattccgttg aagcggttct cgatcgcgtt
      481 acgagtcagg tgcctgccgc acttcggagt tcgagcgcgg tgattcacgg agtggatgag
      541 gggtgggagg tgtgcctatt gaagttcacc attgagatga tgcatcagtc ggccggagga
      601 aatatccagg atttccggcg gaggggcatg ttgtagccgc gcctcagacc agcgagatgt
      661 aagggtatta aggtcgcgtt gggagcagcg tctcatgaat aatctggtga aggtgctgat
      721 tattgccgga ctggcaatcg gcatttttgg gggcggggga tatcttgcgt accggttatt
      781 ctttaagaag ccggacagac tgaagctcgg tgtcaatcat gccgtcgtga ccccgacgcc
      841 tgacgcgggc gtgaccatgt ttgagcaggc gaaccacgaa attgagcacg gagacaaaga
      901 aagcggcgaa aaaatcttgt gggcgctcat tcagaatctt cctgattccg aaaaaaacag
      961 tgatgccaaa aagcttctca gcagcttgaa cgtccaggag tttttctccg ctcagccggg
     1021 tccggacaaa actgagtatg tcgttgcccg gggagactcg atggcgagaa tagccggcaa
     1081 aacgaaatg
//
LOCUS       DATMKH010000256         1047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_121562,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000256 DATMKH010000000
VERSION     DATMKH010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121562"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="VNV63_03070"
     CDS             <1..492
                     /locus_tag="VNV63_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61634.1"
                     /translation="PFIRQHGRRREEGFLEYFCGRLSAQYVLPFRIRYDAHEDRVFGT
                     RTKYYLVHASNSLKAVLLMKEVMWPLGDEEGTFDFSGDSQGVLISEAPQAEELKQILL
                     REFAGQRLASDDIRGKTWKIPFIEKQYRLALQELRAIGKVQVTPVTSKGKGLARRDLI
                     RFL"
     gene            504..>1047
                     /locus_tag="VNV63_03075"
     CDS             504..>1047
                     /locus_tag="VNV63_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020475974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage Gp37/Gp68 family protein"
                     /protein_id="HXC61635.1"
                     /translation="MSVHSSIEWTNATWNPVRGCTKVSPGCKHCYAERFAERFRGVSG
                     HPFEQGFDLRLVPEKLTDPLRWKTPKMIFVNSMSDLFHDGVPDAYIEAVARVMIHARW
                     HTFQILTKRSLRMRDLLSNRLRFAASQPNIWWGVSVEDRHYGLPRVDHLRESPVHVRF
                     LSVEPLLEDLGRVDLQSIDWV"
BASE COUNT          250 a          253 c          290 g          254 t
ORIGIN      
        1 cccttcatcc gtcagcacgg aaggcggaga gaagagggat tccttgagta tttttgcggg
       61 cggctttccg cgcagtatgt gcttccgttc cgaatcagat acgatgcgca tgaagaccgc
      121 gtgtttggaa ccaggacgaa atactatctc gtccacgcca gcaatagctt gaaagctgtt
      181 ctgttgatga aggaagtgat gtggccgctt ggtgatgagg aagggacgtt cgatttttcg
      241 ggcgattctc aaggcgtatt gatctccgaa gcgccacagg ccgaggaatt gaagcagatt
      301 ctccttcgag agtttgcggg acaacgcctg gcatccgacg atattcgagg aaagacctgg
      361 aaaattccat ttattgaaaa gcaatatcgt ttagccctcc aggaactccg ggcaattggc
      421 aaggttcagg ttacgccggt cacttccaag ggaaaaggac tggctcgacg cgatttgata
      481 cggtttttgt aggagcgaat cacatgagcg ttcacagttc gatcgaatgg acaaatgcaa
      541 cctggaatcc ggtacggggc tgcacaaagg taagtccggg ttgcaaacac tgctatgcag
      601 aaaggttcgc cgagcgattc cggggagttt ctgggcaccc ctttgaacag ggcttcgatc
      661 ttcgattagt tccggaaaaa ctcacggacc cactcaggtg gaagacgccc aaaatgattt
      721 ttgtgaattc catgagtgac ctgttccacg acggtgtgcc ggacgcatat atcgaagctg
      781 tcgctcgcgt gatgatccat gctcgctggc atacatttca gatcctgacc aagcgatctc
      841 tccgcatgcg ggacctactc tcgaacagat tgagatttgc agcatctcag cccaatatct
      901 ggtggggagt gagcgtagaa gatcgacact acggcttacc tcgagtcgac cacctgcgtg
      961 agtctcctgt acatgtgcgt tttctctcag tggagcctct acttgaggat cttggcaggg
     1021 ttgacctcca atctatcgat tgggtca
//
LOCUS       DATMKH010000257         1439 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3954353, whole genome shotgun sequence.
ACCESSION   DATMKH010000257 DATMKH010000000
VERSION     DATMKH010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1439
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3954353"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="VNV63_03080"
     CDS             <1..666
                     /locus_tag="VNV63_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61636.1"
                     /translation="GQGHRLVKALRRGFLIGGLSLILMFEVFPTAIGGSWAFVSETLF
                     QGSGGSDLSNRGWDYPVQNLMEAFQEGNIVSGHGTGMSSLGMQYVSRLLGEQIPNISV
                     ESGYGTLIVEMGVFGPILWLLWTSVLLWQGWKVVRKLRESVYFPLGFAIWWYAVIALV
                     LFMHLTMALYQNFVNNAYLWLLLGVLFRLPKLAQMPQPVPMPKHVRALSRWQFASRGS
                     AGV"
     gene            1016..>1439
                     /locus_tag="VNV63_03085"
     CDS             1016..>1439
                     /locus_tag="VNV63_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61637.1"
                     /translation="MRENLGRLDFQIAFCLTLFIGLIACLYTFSFYREESASGWRRTL
                     QSWTSAFDFSLDALGGFSYTGLILASILGLFLELLMIRWMSSEVRIFAFLKNYMLVSC
                     FLGFGLGCFLCRKRINLIALAAPLLTLVFLIQFPWQPLR"
BASE COUNT          249 a          383 c          394 g          413 t
ORIGIN      
        1 gggcagggac atcgcctggt gaaggcgctt cgtcgaggtt tcctcattgg tggacttagc
       61 ctgattctca tgttcgaagt gtttcctacg gccatcggcg ggagctgggc tttcgtttcg
      121 gagacactct tccagggatc tgggggatcc gatctttcca atcggggctg ggattatcct
      181 gtgcaaaatc tgatggaggc ttttcaagag gggaatatag tctccggaca tggtacaggc
      241 atgtcctcgt tggggatgca atacgtctcc cgcttgctgg gtgagcagat ccccaacatc
      301 agcgtcgaaa gcggctatgg aactctcatt gtggagatgg gcgtgtttgg tccaatcctc
      361 tggctcctat ggacttcggt ccttctctgg cagggatgga aagttgtccg gaagcttcgc
      421 gaaagcgtct attttccctt gggcttcgcg atctggtggt acgccgtgat cgcgcttgtt
      481 ctgttcatgc acttgacaat ggccttgtat cagaattttg tgaataacgc ctacctgtgg
      541 ctgctccttg gtgttttgtt tcgcctgccc aaactcgcgc aaatgcctca gcccgtcccc
      601 atgcccaagc atgtccgcgc cctctcccgc tggcaatttg cttccagggg aagtgcaggg
      661 gtgtaattac cgggacgggg gatggggaga acttggcaga ccaaaccagc atcgttcctt
      721 ccttttgcat tcctccattt ggtggtctcc ggtttttcgt tctttccagc ttctcaatcg
      781 caggcatgct ttccgattcc aaggggtctc tgaaccattt tcggaatttt acgtgcgtgg
      841 ggccttcagg acatggccaa atctaatgca tcttcctggc aattgcttcg atcgggctgc
      901 cgttgacacc agagggcggc tggaatatgg ttctgcgcga tataaagttg aaatcggaaa
      961 ttgccgcgca tgctgctcgg catgaatttt cgatgggatt tcccagggag aatacttgag
     1021 agaaaatctt ggacggcttg acttccaaat tgccttctgt ctgacccttt tcattggcct
     1081 gatcgcttgt ctctatacct tcagttttta tcgggaagag tccgccagcg gctggaggcg
     1141 gacgctccaa agttggacct ccgcgtttga tttctcactg gatgctctgg gaggtttcag
     1201 ttacaccggg ctgatccttg ccagcatcct cggcctcttc ctggaactgc tgatgatccg
     1261 ctggatgtcc tccgaggtgc gcatctttgc gtttctcaag aactacatgc tggtctcctg
     1321 cttccttggg ttcggcctgg gctgctttct ttgccggaag cgcatcaacc tcatcgccct
     1381 ggccgctccg ttgctcacgc tcgtttttct gattcaattt ccctggcaac cgctgcgcg
//
LOCUS       DATMKH010000258         1602 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3956288, whole genome shotgun sequence.
ACCESSION   DATMKH010000258 DATMKH010000000
VERSION     DATMKH010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3956288"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1154
                     /locus_tag="VNV63_03090"
     CDS             <1..1154
                     /locus_tag="VNV63_03090"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HXC61638.1"
                     /translation="SISCNLNLDAVLKQIIEIVVQVTRADACLLYLLDGRGEELILRA
                     SKNPHPKLIGRIRLEIGEGITGWVAKEKKPVAIPKNASDDSRFKLFHNLPEDSYQAFL
                     SVPLIVKNEVIGVINVQHKRPHEYNHGEMALLTTIAHQVGGAIENARLYEEMKKKAKQ
                     IETLSQVSRTITSNRYLEEILHLIVTMTAEMMDSKICSIMLLHEERLELEIAATQSLS
                     EAYRKKPPLKIGQSISGLAVKERRPIGVLNVTREASYMYPDLAEKEGLRSLLSVPMML
                     KDRVIGVINSYTSYEHRFTEEEIKLLQAVANQAAVAIENTKLVQRSLQMEEALETRKR
                     VERAKGILAREKKMPEEEAFRLIQKQSMNSRRSMREIADAVILASQLKS"
     gene            1224..>1602
                     /locus_tag="VNV63_03095"
     CDS             1224..>1602
                     /locus_tag="VNV63_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HXC61639.1"
                     /translation="MRTLRIAMAQINSTVGDLPSNTRKICDYITEAKRHHVDFVAFPE
                     LALTGYPPEDLLLKPRFIEDNLSALKNIIKHTQGITAVVGFVDSQDDIYNAAAIIHNG
                     KLAGVYHKIYLPNYGVFDEDRYFQ"
BASE COUNT          415 a          393 c          459 g          335 t
ORIGIN      
        1 agtccattag ttgcaacctg aatctggatg cggttctaaa gcagattatc gagatcgtgg
       61 tccaggtcac acgtgcggat gcctgtctgc tgtacctgtt ggacggtaga ggggaggaac
      121 tgatactccg ggcctcgaag aacccccatc ccaagctgat cggccggatt cgtttggaga
      181 tcggggaggg gatcaccggc tgggtggcaa aagagaaaaa accggtggcg atcccgaaga
      241 atgccagtga cgattcgaga ttcaagttat ttcataacct tccggaagac agctatcagg
      301 cctttttgtc ggttccgttg attgtcaaaa acgaggtgat cggcgtgatc aacgtccagc
      361 ataagcgccc tcatgagtat aatcacggtg agatggccct gctcaccacc attgcgcatc
      421 aagtcggcgg agcgattgag aatgcccggc tttatgagga gatgaaaaag aaagcgaagc
      481 agatcgaaac cctgtctcaa gtgagcagga ctattacttc caatcggtat ctggaggaga
      541 ttcttcatct gatcgtgacc atgacggccg agatgatgga ttccaagatc tgctcgatca
      601 tgctgctcca tgaagaacgg ctggaattag agatcgcggc cacacagagc ctgagcgagg
      661 cctaccgaaa aaagcctccg ctcaagatcg gccagagcat tagcggtctc gcggtgaagg
      721 aaaggcggcc gatcggggtg ttgaatgtga cccgcgaagc gagttacatg tatccggacc
      781 tggcggaaaa agaaggtctt cgctcgctcc tctcggttcc gatgatgttg aaggaccggg
      841 tcatcggtgt catcaatagc tacacctcgt acgaacaccg atttacagaa gaagagatca
      901 aactgcttca ggcggttgca aaccaggccg ctgtggccat cgagaacacc aagctggtgc
      961 agcggtcact acagatggaa gaggctctgg agacccggaa acgagtggaa cgcgccaagg
     1021 gaatcctggc ccgggaaaag aagatgccgg aagaagaggc gttccgttta attcagaagc
     1081 agagcatgaa cagccgccgc tcaatgcgtg agattgcgga tgccgtgatc ctggcgagcc
     1141 agctcaagtc ttaaagaata gcgtccggct ttgccggcgc ggggactggg gggcggtccc
     1201 cagatataaa aaggttatat aagatgcgta cacttcgaat tgccatggcg caaatcaata
     1261 gcaccgtcgg cgatctgcca tcgaatacac ggaagatctg cgactacatc acggaggcca
     1321 agcgtcacca tgtggatttc gtggctttcc ctgagcttgc gctgaccggc tatcctccgg
     1381 aggacctgct tttaaaaccc cggttcattg aagacaacct ctccgctctc aaaaatatca
     1441 tcaagcacac acagggtatt acggccgtgg tgggcttcgt ggacagccag gatgatatct
     1501 ataatgcggc agccatcatc cacaatggaa agttggcggg ggtctatcac aagatctatc
     1561 ttcccaacta cggggtgttt gatgaggatc gttattttca gg
//
LOCUS       DATMKH010000259         3212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4291212, whole genome shotgun sequence.
ACCESSION   DATMKH010000259 DATMKH010000000
VERSION     DATMKH010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4291212"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="VNV63_03100"
     CDS             <1..370
                     /locus_tag="VNV63_03100"
                     /inference="COORDINATES: protein motif:HMM:NF013854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HXC61640.1"
                     /translation="AGEHATFSDNRLKLLQELKGISGLKRKALFRCIIVLASPTLVLA
                     AEEGVLSGRIAVQERGQKGFGYDSILLLPGRRRTLAEMTPEEKNRISHRARALAKIKR
                     HLKRLVSKDPSQQRGVAQLG"
     gene            347..424
                     /locus_tag="VNV63_03105"
     tRNA            347..424
                     /locus_tag="VNV63_03105"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:382..384,aa:Pro,seq:tgg)
     gene            436..512
                     /locus_tag="VNV63_03110"
     tRNA            436..512
                     /locus_tag="VNV63_03110"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:470..472,aa:Arg,seq:tct)
     gene            623..700
                     /locus_tag="VNV63_03115"
     tRNA            623..700
                     /locus_tag="VNV63_03115"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:658..660,aa:His,seq:gtg)
     gene            838..1506
                     /locus_tag="VNV63_03120"
     CDS             838..1506
                     /locus_tag="VNV63_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXC61641.1"
                     /translation="MRILVVEDEEKVARFVRRALEEEHYAVDLASDGESALEQIDVVP
                     YDLVILDLTLPKKGGFELLHLLRQQGNKVPVLILTARTAVSYRVKGLDLGADDYLVKP
                     FAIEELLARVRALLRRGGSEPITKLQAEDLILDPVSHEVYRSGKKIDLTTKEYALLEY
                     FLRNPNRVLTRSMITEHVWDIHFDTFTNVIDVYVSYLRNKVDRGFKRPLIHTVRGVGY
                     VLKV"
     gene            1490..2926
                     /locus_tag="VNV63_03125"
     CDS             1490..2926
                     /locus_tag="VNV63_03125"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXC61642.1"
                     /translation="MSSRSESRRFPWRHSIRTRLALWYGGVLGAAIVFMGVILYSYLS
                     ENLYSDFDLSLHSTAEALSRSSLSLPLLPGLNADTLLDGMEDPEFFSKFFQFFDPSGN
                     FGFRSKNLPKQNLPLTQIALNNALKGEMTFETFSSESQGLVRVLTYPVVQEGKLVHVL
                     QVGGSLRHIERVLMRLRMILFLILPIVLILALSGGWFLTHQVLEPVDAMAEVARQITA
                     GDLSRRIPVYKGRDELARLAETFNTMIERMEDSIQRLRRFYANTSHALRTPLTILKGE
                     AEMALRQARTVKEYQQTLASGLEEIDRISKIVDDLFTLSKAGLEEVRLEMKPVQLDSL
                     VAETVSHMELFAQDKKVGLVLDCNEPVIITGDMERLRELLFNLIENAVQYTPAGGRIA
                     VSLVREEKNALIIVTDTGIGIPEEDLPKIYDRFYRSNEAHAMNPKGGGLGLPICLWIV
                     TSHNGQIDAESKLGKGTKFTVRFPLLSD"
     gene            3030..>3212
                     /locus_tag="VNV63_03130"
     CDS             3030..>3212
                     /locus_tag="VNV63_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61643.1"
                     /translation="MQRIYLGVMLILTCLAVGLFSCAGGVPKTSDEQIVDGSLARLKM
                     DSRINADNIHVESKNYI"
BASE COUNT          765 a          770 c          856 g          821 t
ORIGIN      
        1 cgccggggag catgcgactt tttcagacaa ccggcttaag ctcttgcaag agctaaaagg
       61 catatcggga ttaaaacgta aggccttgtt ccggtgcatt atcgtattgg cgtcgcccac
      121 tcttgtgctc gcggccgaag aaggcgtgct gtctggcagg attgcggttc aggagagagg
      181 gcaaaagggt tttggatacg actccattct tctgctgcct gggcgtagaa gaacactggc
      241 cgagatgaca ccagaagaga agaatcggat cagccaccgc gcaagagctc tggcgaaaat
      301 aaaacggcac ctcaagcgac ttgtttctaa agatccgtcg caacagcggg gcgtagcgca
      361 gcttggttag cgcgcctgcc ttgggagcag gaggtcggcc gttcaaatcg gcccgccccg
      421 accagaaaaa ctcgggcgcc cgtagctcag gcggatagag catctgcctt ctaagcagag
      481 ggtcgcaggt tcgaatcctg ccgggcgcgc cagaaacagt taagaaattg taaaccgcga
      541 atttgcacgt tgtgtgtctt cacttaaaag ttgtagcgtg caacaggtcg cgtgtaactt
      601 gtaaccgttt tttattatca tggtgagcgt agctcaactt ggttagagca ctggcctgtg
      661 gcgccagggg ttgggggttc aagtcccctc gctcacccca aattttgttc ttttctcacc
      721 ggccattgac aggttcttga gagaatcctc gtaagagtca cgcccggccc agccgctata
      781 tctctacaac cgcctgagag atttactctc gaataagggg tatgatatac tcgccgcatg
      841 aggattttgg tcgtggagga tgaagagaaa gtcgcccgtt ttgtccgacg cgccttggaa
      901 gaagagcatt acgctgtgga tcttgcttcg gatggagaat ccgcacttga acagatcgat
      961 gtggttcctt acgacttggt cattctggat ctaaccttac caaaaaaagg cgggtttgag
     1021 cttcttcatc tgttgcggca gcagggcaat aaagtcccgg tcttgatcct taccgcccga
     1081 actgcggtgt cgtaccgtgt gaagggtttg gacctgggag cggatgacta tttggtcaag
     1141 ccgtttgcga tcgaggagct gttggcccgc gtgcgcgcac tattgcgccg tggaggaagc
     1201 gagccgatca caaaacttca agcggaagat ctgatcctgg atccggtatc gcatgaggtt
     1261 tatcgctccg gaaagaagat tgatctcacg acgaaagaat atgccctgct ggaatatttt
     1321 ttgagaaatc ccaatcgtgt tcttacgcgg tcgatgatca cagagcatgt atgggatatt
     1381 cactttgaca cctttaccaa tgtgattgat gtctacgtca gctatcttcg aaacaaagtt
     1441 gataggggtt ttaaacgccc tctgatccat accgtacgag gggttggcta tgtcctcaag
     1501 gtctgaatcg cgacggtttc cctggaggca ttcgatccgc acccgcctgg ctttatggta
     1561 cggcggtgtt ttgggggcgg ccattgtctt tatgggtgtc attctttatt cttacctttc
     1621 cgaaaacctg tattctgatt ttgatctctc tctccactca accgcagagg ccttatcacg
     1681 ttcctcattg agtctgccgc ttctaccggg attaaatgca gatactcttt tggatggaat
     1741 ggaggaccct gagtttttca gcaagttctt ccagttcttt gacccttcag gaaacttcgg
     1801 gttccgttcg aaaaatctgc cgaagcaaaa tcttcctctg acccagattg cattgaacaa
     1861 tgctcttaaa ggagagatga cgtttgaaac attttcaagc gagagccaag gactggtgcg
     1921 tgtgctgacc tatccagtgg tacaggaagg gaaattggtc catgtactac aggtcggagg
     1981 atcgctacga cacatcgaga gggttttgat gcggcttcgc atgattcttt ttctcatcct
     2041 tccgatagtc ctcatcctcg cgctgtccgg cggatggttt cttacacatc aggttcttga
     2101 gcccgtggat gccatggctg aggtcgcgcg gcagattaca gcgggagatc tgtcgcgcag
     2161 gattccggtt tacaaaggcc gagacgagct cgcgcgcctg gcagagactt ttaatacaat
     2221 gattgaacgg atggaggatt cgatccagcg cctccggcga ttctacgcca atacgtctca
     2281 tgcactgcgt acaccactta cgattctcaa gggggaggcc gaaatggcct tacgacaggc
     2341 ccggacagta aaagaatatc agcagacgct tgcgagcggg ctggaagaaa tcgatcggat
     2401 ttcaaagatc gtggatgatc ttttcactct ttccaaagcc ggcttggagg aggtccggct
     2461 ggagatgaaa ccggtccagc tcgattccct agtggccgaa acagtttcac atatggagct
     2521 tttcgctcag gataagaagg tagggcttgt tcttgattgc aatgagcccg tcatcatcac
     2581 cggtgatatg gagcggctgc gtgagctgct gttcaatttg atcgagaatg ccgttcaata
     2641 tacaccggcc ggtggaagaa tcgccgtctc tcttgttcgg gaagaaaaaa acgccttgat
     2701 catcgtaacg gatacaggaa tcggaatccc cgaagaagac cttcccaaga tctacgatcg
     2761 tttctaccgc tctaatgaag ctcatgctat gaaccccaaa gggggcggct tgggccttcc
     2821 catctgctta tggatcgtca cctcccataa cggtcaaatt gatgcggaaa gcaagctcgg
     2881 caaagggact aaatttactg tccggtttcc gcttctctcc gattaaacat ctttaatctc
     2941 tgattaatag ggaatcccgg ttctcattag aaaactttaa tgtccgatta acaggttcct
     3001 aacgttatcc gcctaggata gactatgcta tgcaaagaat ctatttggga gtgatgttga
     3061 tactgacgtg tcttgctgtg ggtctcttta gttgcgcagg aggcgtccca aaaacatcgg
     3121 atgaacagat tgtagacggc agtcttgcgc gcctgaaaat ggacagccgg atcaatgctg
     3181 ataatattca tgtggaaagc aaaaattata tc
//
LOCUS       DATMKH010000260         1025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_445460,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000260 DATMKH010000000
VERSION     DATMKH010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445460"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..>1025
                     /locus_tag="VNV63_03135"
     CDS             103..>1025
                     /locus_tag="VNV63_03135"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HXC61644.1"
                     /translation="MTAHSVQKSKKAVKNKSSVLATQVSELLDQYNRMGETGEPFGTV
                     VIAEEGQKLEEHGADVIFCAESILSTAVPDVVLDETQKTLREVAVKQEAPFLGIPELR
                     RAIAARFERLYDYTVNWEDQIIVTSGSMQAEYYLMSALLNPGDEVIVPVPSFFFDIPV
                     KLARGKAVLAKLNPKKNYYHDRKDLEKLVTRKTKLITLCNPHNPTGRVLTEEELSGIA
                     ELAQKRDLLIMHDQVYERMVYDGRPYTPMCRIKEVRDRLITVSSFSKLFNMINYRLGF
                     AAGPKEIIGGMEMVHAFSSMGNPSLIQKGAVV"
BASE COUNT          272 a          253 c          278 g          222 t
ORIGIN      
        1 agatcttcgg gcgaactaaa taactccctc ccaatttccc gtgtttgcgg gatttctcta
       61 cccttaacag tttaaaggag cgaatttgca acagtatatt tcgtgacggc gcactccgtg
      121 caaaaaagca agaaagcagt caaaaacaaa tcgagcgtgc tggcgaccca ggttagcgag
      181 ttactggatc agtacaatcg aatgggcgag acgggcgagc catttggaac cgtcgtcatt
      241 gccgaggagg gccagaagct ggaggagcat ggggcggacg ttattttctg cgccgaaagt
      301 atcctgtcca ccgcggtgcc ggacgttgtc ctcgacgaga ctcaaaagac cttgagagag
      361 gtcgcggtaa agcaggaagc gccgtttctc ggaattcctg agctgcgtcg cgcgatcgcg
      421 gcgagatttg agcggcttta tgattacaca gtcaactggg aagatcagat catcgtcacc
      481 tccggaagca tgcaggcaga gtactatctg atgagcgcgc ttttgaaccc cggcgatgaa
      541 gtcatcgtgc ccgttccgtc cttctttttc gatattccag tgaaactggc gagaggcaaa
      601 gccgtacttg ccaaattgaa tcccaagaaa aattattatc atgacagaaa ggacctagaa
      661 aagctcgtca ctagaaaaac gaaactcatt acattgtgca acccgcacaa ccccacgggt
      721 agagtactaa cagaagagga gctttcaggg atcgcggagc ttgcccaaaa gagagacctg
      781 cttatcatgc atgatcaggt ctacgaaagg atggtctatg acggcaggcc gtacactccc
      841 atgtgcagga ttaaggaagt gcgggaccgg ctcattacag tctcctcctt ttccaagctc
      901 ttcaatatga tcaactaccg gctgggcttt gctgccggcc ccaaagagat catcggcggg
      961 atggagatgg tgcatgcctt ttcgagcatg gggaatccca gcctgatcca aaaaggggcg
     1021 gtggt
//
LOCUS       DATMKH010000261         1332 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1403699, whole genome shotgun sequence.
ACCESSION   DATMKH010000261 DATMKH010000000
VERSION     DATMKH010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1403699"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1223)
                     /locus_tag="VNV63_03140"
     CDS             complement(<1..1223)
                     /locus_tag="VNV63_03140"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HXC61645.1"
                     /translation="MALLVKRICMIVFVLLVCGALSVAQTMPTLEVRRKALNDLLAEQ
                     WEYYLRTNPLDASSLGDKRWNDKLDDFSQEFIDKDLLETQKFLVRFEAIATTGFPKQE
                     ILNKRLMVRDLKMSLEGARFKGWEMPETQQSGYHLYLPQLVLILSFQTVKDYEDYIAR
                     LNQVPRLFDQTIIQMRKGMADKLMPPIFLLAKVGDQTTGIANQAPVDSPFAKPFKDFP
                     KSISEKDQARLRDQGIAAIRGAVLPAYAHFAKFVINDYAPHGRTEVGVWSLPDGDNYY
                     AFRVKRSTTTDLSPEQIHQLGLAQVKEIEARMNEVAAQLGYKDRKLFNAAIAADPKLH
                     AHSRQQILDLYTKYIDQMNPKLPELFNRLPKAKLVVLPVEEFREKEASAASYQDPAMD
                     GSRPGHVMVNTGDFE"
BASE COUNT          304 a          341 c          361 g          326 t
ORIGIN      
        1 ttttcaaaat ctccggtatt gaccatgacg tggccggggc gcgagccatc catcgccgga
       61 tcctgatacg aagcggcaga tgcttccttt tccctgaact cttccaccgg caacactacc
      121 aatttcgctt tggggagacg gttgaacagc tcgggtagtt tgggattcat ctggtcgatg
      181 tatttggtat agagatccag aatctgctgg cgcgagtggg cgtggagttt cggatccgcg
      241 gcaatagctg cattgaacaa tttccggtct ttgtatccca attgagcggc gacctcattc
      301 atgcgcgctt cgatctcctt cacctgggcg agacccagct ggtgaatctg ttccgggctg
      361 agatccgttg tggtacttct tttcactcga aacgcatagt agttgtcgcc atcggggagc
      421 gaccacacgc cgacctccgt gcgcccgtgc ggggcgtagt cattaatcac aaattttgcg
      481 aagtgcgcat aggcgggaag gacggcccct cgaatggcgg caattccctg gtctcggagg
      541 cgagcctgat ctttctcgga gatggatttg gggaaatcct tgaagggttt agcgaaggga
      601 gaatcgacgg gcgcctggtt ggcaatccca gtggtctgat ccccaacttt cgcgagaaga
      661 aaaatagggg gcattaattt gtcggccatg ccctttcgca tctggatgat cgtctgatcg
      721 aacagccgag gcacctggtt caggcgagcg atgtaatcct cgtaatcctt gacggtttga
      781 aacgagagga tcaacacaag ttgagggaga tacagatggt atccgctttg ctgggtctcg
      841 ggcatctccc accccttgaa acgagctccc tccagcgaca ttttaagatc gcgaaccatg
      901 aggcgcttgt tcaagatctc ctgtttgggg aagcctgtcg tggcgatggc ttcaaaccgt
      961 actagaaatt tctgcgtttc gagtaaatcc ttgtcgataa actcttgcga aaaatcatcc
     1021 agcttatcgt tccaacgctt gtccccgagc gaagaggcat ccaatgggtt ggtgcggagg
     1081 tagtattccc attgctcagc aagaagatcg ttaagggctt tgcgcctgac ttccagtgtg
     1141 ggcattgtct gcgctacgct cagagcgcca caaaccagga gaacgaaaac aatcatgcaa
     1201 atgcgtttta caagtaaagc catggggcct cctaccgtaa ggtgaatcca aacgtaaggg
     1261 agaggaacgc tattcagccc ggaatttcct ttgatctcga aacgtcacca tactatacct
     1321 ctctacaccg ac
//
LOCUS       DATMKH010000262         4540 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_764031,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000262 DATMKH010000000
VERSION     DATMKH010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4540
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_764031"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3787)
                     /locus_tag="VNV63_03145"
     CDS             complement(<1..3787)
                     /locus_tag="VNV63_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018048551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HXC61646.1"
                     /translation="MALILFFSAQGWAISGEMADNISPDSSDIQKASDPSWQSRLKDQ
                     INREDSQEGRTGHSDQVDAAMQKLMGEISKGANEHAAHTGNPGPFSDMSMMQQMDRSF
                     FLGPTGVSESVTGGGHCPSNAQVKEYDISAINVEITINQWLDYFPGYMYVLTDNLQKV
                     RDEEKINREARKKPGYDPGAVSNGLQTDMIQPLNIRGNQGDCIIITLRNQMKDDDVSL
                     HIHGSSMIVKTTGQPATMANPDSIVKPGKNQVFEWYIRPEDQEGGHTLHSHVGREQAS
                     LGMIGSFIIEPLGSRYLDPLTGKETKSGWQVMIDQTNSPDFREFVIIYHEIGDESFRP
                     LNRNGEMIPQRDPNTDAYRPSARAINYRSEPFGVNNLALQEKYFHHEDESLGYSSYTF
                     GDAPTTIPRSYMGDPAKFRLVHGGGEVFHSHHPHGGSIRWLRQPKADGKADFLMTAAG
                     NGPVKFPEIRTTSDRVDVQVIGPSEVLDLQTECGSGLCQQLAGDFLFHCHVAHHYVAG
                     MWGYWRVYNTLQNGNYPLASTDIMPPLQELPDRKGRMKSAVTSDKLVGKMMDWYDKKW
                     NITQDKSDWSKPIPDVAIKDWVKMMIPPAGQPGHTADEKGQIEAYDATVWDWNWEGNK
                     ALGEPEVTGTFDWPKYKSPMPGKRPPLLFDAKTGKLAWPHFKPHFGKRVPFARHHGPA
                     PWLEPIHMEKENGTLTSDPGGKGAAGQETTQPAKPGEQGRWSLCPENAGRKQYTIHFI
                     ETPIQLTKGLGKEKPVVDPDGLLYVLQEEEAEVKANPGGDKTIPLVYRASVYDCVDVI
                     LKSEWNDNDTQNFQMSKINIHIHFIQFDNQASDGVITGFSYDQSVRPFTMIGKKSQKG
                     LPPPMNALLTEDTKAGSNSIHIKMGEGATPFHVGTDLMIGMKEVKTSEIRWIQDIKGD
                     TVTFTEPLKFDHKKDEIASVEWVRYRYWVDADTGTVFWHDHAFGATTWPHGGVGAMIV
                     EPAGSTYHDPKTGAPIRSGPIADIHSTEPIGYGINGSFRELVVMPHDTVPMTAQVVST
                     GNPPGQTVQVAIDAGQTLSFKMPYDLDKVAIPMLNGGTHTTGGGFNFRAESIARRLKL
                     NPDPSLVFSSKAHQDPSTPLLRAYLGDTIAFRLLHTLMNETHVWHVAGHAFRTERYAE
                     KSDFRNAHHVGIAERYDLITKAGGPQQMAGDYLHYNGRASHLSEGSWGIIRVLDKEIP
                     DLKKLPGRDEIPASAKSVCPADAPVKSFNVIAVDHALKFNPKAPDLIDVDFDRKLQVA
                     "
     gene            complement(4146..>4540)
                     /locus_tag="VNV63_03150"
     CDS             complement(4146..>4540)
                     /locus_tag="VNV63_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61647.1"
                     /translation="DQFQSLYRQLATPKERKKPFGMGSKELPDHDASAVQVLSHKWHP
                     DTVWERIGKTKFVWEATVRNNSDTRKRVFVYYDLLDENNFPLARNMANQFIEPHQTIV
                     VASDSYIESVELPRVKTSRATIKIGSTH"
BASE COUNT          987 a         1200 c         1146 g         1207 t
ORIGIN      
        1 tcgctacttg aagcttgcga tcaaagtcca catcgattaa gtccggggcc ttggggttaa
       61 atttcaaggc atgatccacc gcgatgacgt tgaaactctt cacaggcgca tcagccgggc
      121 agaccgactt ggctgaggcc ggtatttcat cccggcccgg aagcttcttc agatcgggaa
      181 tttctttatc taagacccgg atgattcccc agctcccttc cgagaggtga gatgcccggc
      241 cgttgtaatg aagataatcc ccggccatct gttgtggccc accggctttg gtaatcagat
      301 cgtaccgctc tgcaatcccg acatgatggg cattgcggaa gtccgatttc tcggcatatc
      361 gctctgtccg gaaggcgtgg cctgcaacgt gccagacatg ggtctcgttc attaaggtgt
      421 gcaggagtcg gaaggcaatt gtgtccccca gataagcacg cagaagcggt gtactcgggt
      481 cttggtgggc tttgctggag aaaacaagcg atggatcggg gttgagcttc aacctccttg
      541 caatggactc tgcccggaag ttgaagccac cgcctgtcgt atgggttccg ccgttgagca
      601 tcggaatagc aactttatcc agatcatacg gcatcttgaa cgagagggtc tggccggcat
      661 caatcgctac ttgaaccgtt tgacccggag ggttccctgt gcttacaacc tgggctgtca
      721 tcggtacggt gtcatgcggc atgacaacca attctctaaa gcttccgttg atcccatagc
      781 cgattggctc agtggaatgg atatcggcaa tcggaccgct tcgaatcggc gccccggtct
      841 tcggatcatg gtaggtcgaa ccggccggtt caacgatcat tgctccgacc ccaccgtgag
      901 gccaagtggt tgcgccaaag gcgtgatcat gccagaaaac ggttcctgta tcggcatcca
      961 cccaataacg gtaacggacc cattctaccg aggcgatctc atctttttta tgatcaaatt
     1021 tcaacggttc cgtaaaagta acagtatctc ccttgatatc ctgaatccag cggatctccg
     1081 aggtttttac ttccttcatg ccgatcatca agtctgtccc aacgtggaag ggagtcgccc
     1141 cttcacccat tttaatgtgt atactgttcg atcctgcctt agtatcctca gtcagaaggg
     1201 cgttcatcgg aggagggagc cctttttgcg acttttttcc gatcatcgta aatggccgga
     1261 ccgactggtc ataggaaaat ccggtgatca ccccatcgga ggcctgattg tcgaactgga
     1321 taaaatgaat atggatattg atcttcgaca tctggaaatt ctgcgtgtcg ttatcgttcc
     1381 actcgctctt gaggatgacg tccacgcaat catagacact ggcgcggtag accaatggga
     1441 ttgtcttgtc accgccagga ttggccttga cctcggcctc ttcctcttga agtacataga
     1501 gcaggccgtc tggatccacg accggttttt cttttcccag ccctttggtc aactggatcg
     1561 gggtctcgat aaaatgaatt gtgtattgct tccggcccgc gttttcagga caaaggctcc
     1621 accgaccctg ttcacccggc ttggccggct gggtggtttc ctgccccgca gctccctttc
     1681 cacccggatc gctggtcaat gtcccattct ctttttccat gtggatcggt tccagccagg
     1741 gggctggtcc atgatgccgc gcaaacggaa cccgcttccc aaaatgcggc ttaaaatgag
     1801 gccaggccag cttcccggtt tttgcatcga aaaggagagg aggtctcttt cccggcatcg
     1861 gcgatttgta cttcggccaa tcaaacgttc cggtcacctc gggctcgccg agagccttgt
     1921 ttccttccca gttccagtcc cagaccgtcg cgtcataagc ctctatctga cccttttcat
     1981 ctgcggtgtg tcccggctgt ccggcgggag gaatcatcat cttgacccaa tctttaatcg
     2041 cgacgtccgg gatcggcttt gaccagtcgc ttttgtcttg agtgatgttc catttcttat
     2101 cgtaccagtc catcatcttg ccgaccagtt tgtcggaggt gaccgcggac ttcatacggc
     2161 ctttacggtc tggaagctcc tgcaacggcg gcataatgtc agtgcttgcg agaggatagt
     2221 tgccgttctg aagcgtgtta tagacccgcc agtaacccca catcccagcc acataatggt
     2281 gcgccacatg gcaatggaac agaaaatctc ccgccagctg ctggcagagt ccggagccgc
     2341 actcagtctg aaggtcaagg acttctgaag gaccgatcac ctgaacatca actcggtcag
     2401 aggttgtccg gatttctggg aacttcaccg gcccgtttcc ggctgcggtc atgaggaagt
     2461 cagcctttcc atcagccttc ggttgacgta gccagcggat cgacccgcca tgggggtgat
     2521 gggagtggaa gacctctcct cccccatgga ccagacggaa cttcgcggga tcacccatgt
     2581 acgaacgggg aatggtcgtg ggggcgtcgc cgaaggtata ggagctgtac cccaaggatt
     2641 cgtcttcatg atggaagtac ttttcttgaa gggcgagatt attgaccccg aacggctcgc
     2701 tgcgataatt aatcgcccgt gctgatggac gataggcatc tgtattgggg tcgcgctgcg
     2761 gaatcatctc gccgttccgg ttcagcggcc ggaaggactc atcaccgatc tcatgataga
     2821 tgatcacgaa ctcccggaag tcaggcgagt tggtctgatc aatcatcacc tgccagccac
     2881 tcttggtctc ttttccggtg agcggatcca gataacggga tccaagcggc tcaataatga
     2941 aactaccgat cattccgaga cttgcctgtt cacgaccaac gtgactgtga agggtgtgtc
     3001 ccccctcttg atcttccggc cgaatgtacc attcgaatac ttggttcttt ccgggtttga
     3061 cgatagaatc gggattggcc atcgtggctg gctgaccggt tgttttcacg atcatgctgg
     3121 agccgtggat gtgaaggctt acgtcatcgt ccttcatttg attccgaagg gtgataataa
     3181 tgcagtctcc ttggttccct cggatattca gcggctggat catgtcggtc tgaagaccgt
     3241 tcgaaacagc accaggatca tatcccggtt ttttacgggc ttctcgattg atcttctctt
     3301 catcacgaac cttctggaga ttatcggtca ggacatacat gtacccggga aagtaatcca
     3361 gccactgatt gatggtgatc tccacattga tggctgatat atcatactcc ttgacctgag
     3421 cgttgctggg gcaatggcct cctcccgtga cgctttcgct aacccctgtc ggtccgagaa
     3481 agaaactccg gtccatctgc tgcatcatgc tcatatcgct gaagggtcct ggattgccgg
     3541 tgtgcgccgc atgctcattg gctcctttgc tgatttcacc catcagcttt tgcatggcgg
     3601 catccacctg gtctgaatgg cctgtacggc cttcctgact atcttcacga ttgatctgat
     3661 ccttcaacct gctctgccaa gacggatccg aagccttctg aatatctgaa ctgtctgggg
     3721 aaatattgtc agccatttct ccactaatcg cccatccctg agcagaaaaa aacaggatga
     3781 gagccaatag ccctccacaa agatttgttc tagcactcat atatgtttct ccctgggtat
     3841 tgttttacag ttttttggat ccctagattc atcagaactg gtttgctttc aacctttgat
     3901 tttgactaga cccatggcca catcttcggt tttatagtgg gggacatcca agcaatggtt
     3961 gctgaccgcg gggtaacgtg ccttgacatt gcttaatctc ggtattcata aaggaattat
     4021 tcgaattcag tactgtacaa ttgaataaac atttgtccag cttggtagac aattattgtc
     4081 gcactgatga aatgatcttg tcaagggata aaaattttga tttttaaaat cggagagttg
     4141 aaatgttagt gcgtagaacc gatctttatg gtcgcacggc tagttttgac ccttggaagc
     4201 tcaacactct caatataaga atcagaagca acaacgatgg tttgatgagg ctctataaat
     4261 tgattggcca tattacgagc caaagggaaa ttattttcat ccaaaaggtc gtagtaaaca
     4321 aagacccttt ttcgggtatc ggagttgttt cgaacggtag cttcccagac aaatttggtt
     4381 ttgccgatcc tttcccaaac agtgtccgga tgccatttat ggcttaaaac ctgtactgct
     4441 gaagcgtcgt gatcgggtag ttccttgctc cccatcccaa acggcttctt tcgctccttt
     4501 ggcgttgcca attgacggta tagcgattgg aactggtccg
//
LOCUS       DATMKH010000263         1391 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2046321, whole genome shotgun sequence.
ACCESSION   DATMKH010000263 DATMKH010000000
VERSION     DATMKH010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1391
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2046321"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..1015)
                     /locus_tag="VNV63_03155"
     CDS             complement(173..1015)
                     /locus_tag="VNV63_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HXC61648.1"
                     /translation="MTRKALGKGLEALLGGLPIEDSGRAERLIEIDVHRILPNRYQPR
                     QTFDDTGLQELAASIKAQGILQPVSVRRGEDGRYELIAGERRWRAAQLAGLRRIPALV
                     RETTDRDLVELALVENLQRKDLNPMESAQAYQRLMKEFHLSQEEISKRVGKERSSVAN
                     HLRLLNLPGPIQEALRQERLTMGHAKAILSLSEAKAQWTLAQRILDGGLSVREAERRA
                     QQIKGVAVKTHRKSREIAAQEERLKHYLGTNIRIVPGAKGGRIVIDYYSAEDLERILE
                     MIIQ"
     gene            complement(993..>1391)
                     /locus_tag="VNV63_03160"
     CDS             complement(993..>1391)
                     /locus_tag="VNV63_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020678230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HXC61649.1"
                     /translation="PPSLGLLTMNALVAADSVLVPLQCEYYAMEGLGHLLKTVRAIQK
                     AYNPRLTIEGILLTMYDGRSNLSDQVEQEIRSHFQQKVFRAFIPRNITLAEAPSHGRP
                     ALLYNVGSKGAQAYLRLAKEVLSHDKKSVG"
BASE COUNT          283 a          414 c          341 g          353 t
ORIGIN      
        1 taatgatctc tccctcgact cgtccatcaa tccgcacggt accttcatag gaaatgaccc
       61 ctttaaattc tgtccctttt cctaaaaatg caatgatttc atcgggattg agtggctgcc
      121 tgtcccgtcc ctctttattg aacatattcc cacgcctcct ttgatgaaac ccttattgaa
      181 tgatcatctc caggattcgc tctagatctt ctgcagaata gtaatcaatc acgatccggc
      241 cgcctttagc cccgggtacg attcggatgt tcgtaccgag gtaatgcttc aatcgctctt
      301 cctgcgcagc gatttctcgg ctcttccgat gcgttttgac agcgactccc ttgatctgct
      361 gtgctctcct ttcggcctcg cgaaccgaga gacctccatc gagaatgcgc tgtgccaggg
      421 tccattgagc cttcgcctcc gataaggaca aaatcgcctt ggcgtggccc atcgtcaacc
      481 gctcttgacg gagcgcttcc tgaatcggtc ccgggaggtt taagagcctc aagtgattag
      541 ccaccgaaga ccgctctttc cctactcttt tcgatatctc ttcttggctc aagtgaaact
      601 ccttcatgag ccgctgatag gcctgggctg actccatggg gttgaggtcc ttccgctgaa
      661 ggttctcgac cagtgccagt tcgaccaggt cccgatccgt agtctctctg acaagggccg
      721 gaatgcgtct tagcccggcc aattgagccg cccgccatcg ccgttcaccc gcgatcaatt
      781 catatcgacc gtcttccccc ctccggaccg agaccggctg cagtatgccc tgggccttga
      841 tcgaggcggc aagctcctga agccccgtgt catcaaaggt ctggcggggt tgatagcgat
      901 ttggaaggat gcggtggaca tcgatctcga tcagcctttc agcccgaccg gaatcttcga
      961 tgggaaggcc ccccaatagg gcctctaacc ctttacccaa cgcttttctt gtcatgacta
     1021 agcacttcct tggccagcct gagataggcc tgcgcgcctt ttgacccaac attgtaaagg
     1081 agagccggtc tcccgtggct cggggcttcc gcaagagtaa tgtttctcgg aataaaggca
     1141 cgaaagacct tttgttggaa atgactgcgg atctcttgtt cgacctgatc actcaaattg
     1201 cttcgaccgt catacatcgt caatagaatc ccttcaatgg tcagccgcgg gttgtaggcc
     1261 ttctgaatcg cccgaactgt tttgaggaga tgccccagcc cttccatggc gtagtattcg
     1321 cattgaagcg ggacgaggac cgagtcggcg gcaaccaggg cgttcattgt caaaagccct
     1381 aaggaaggcg g
//
LOCUS       DATMKH010000264         2636 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4292199, whole genome shotgun sequence.
ACCESSION   DATMKH010000264 DATMKH010000000
VERSION     DATMKH010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4292199"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="VNV63_03165"
     CDS             <1..278
                     /locus_tag="VNV63_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HXC61650.1"
                     /translation="YIFTAAMKEGLIALVAIGGVNIVISLYYYLVVVKKIYINPAKDA
                     SAIPVPFPLKAVVMITIAGVLILGIYPGPVMDWTATATELFSHLTGP"
     gene            327..1010
                     /locus_tag="VNV63_03170"
     CDS             327..1010
                     /locus_tag="VNV63_03170"
                     /inference="COORDINATES: protein motif:HMM:NF015961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HXC61651.1"
                     /translation="MTSGLKLFFQQIVIFLLFCFNWLLIWFYRLTCRIEVVGDLDAVM
                     NQPDPVLLAGWHQDMLCTFSYLTRYARRRPIAVLVSRSKDGEVAAGVLSWFGFVPIRG
                     SSSRGGAAALAQLCDRVKIDRSIAAIICDGPRPPARVAKPGIITLSRLTGRPIFLLRS
                     WSTRQHIFEKSWPRLALVLPFSRIVVMIEGPLSVPPRASDEEQERLRLELQDRLNRLA
                     EASEKYFIR"
     gene            1127..2083
                     /locus_tag="VNV63_03175"
     CDS             1127..2083
                     /locus_tag="VNV63_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61652.1"
                     /translation="MADKTVWILDDYQERSVSQKAAPPTRLEKSPAVAFSLSLLFWGG
                     GQIYNRQWGLGFLFILIMANVYGTLAIAILFWDFITSSLKSVYITSFEVFAACGIFYL
                     FGLIFWAFNAFHAYHKATQARTDPFQGIDNRLLPPLCSALIPGWGQFLNGQSKKGVFF
                     LIFAMAGQFVLAAFLLIPLFWPALETDMDRLMLEKVLAIAVILSPLILLMWGFSIYDA
                     VRVCLEPLKKEPLRKRFEYAINRIRIKGWKQAVVSRAKPIFVLGLFLVLSLTLGYYYF
                     PQREYVTMLQSLRFQLSEQKMVLIPDLIGQFVQTISPEAPRH"
     gene            2095..2427
                     /locus_tag="VNV63_03180"
     CDS             2095..2427
                     /locus_tag="VNV63_03180"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HXC61653.1"
                     /translation="MIDLYSPQNEVELALLKSILDAESINYFVRNDNFGSMQVGPQID
                     LYNKKMIVVQDNQYESAKELVADYLERTEKKIEEPEKNYSVFDKIRMTLEVLLFGWVM
                     PGRKNKGA"
BASE COUNT          593 a          663 c          674 g          706 t
ORIGIN      
        1 tttatatttt cacggcggcc atgaaggaag ggctcattgc actggtggca atcggggggg
       61 tcaatatcgt gatctcgctc tactactatc tcgtcgtggt caagaagatc tatatcaatc
      121 cggccaagga cgcctccgca atcccggtgc cgtttccgtt gaaggcggtg gtcatgatca
      181 cgatcgcagg cgttctgatt ctagggatct atcccggtcc ggtgatggac tggaccgcca
      241 cggccaccga gcttttctcc catcttacgg gtccgtgaca ggctttaatg tcaggcttgg
      301 atagcaggct tggatagcag gcttggatga ccagcggtct taaattattt ttccagcaga
      361 tcgttatttt tcttctcttc tgtttcaact ggctgttgat ttggttctac cgtctgacct
      421 gtagaatcga agtcgtcggc gatctcgatg ccgtcatgaa tcagcccgat cccgtcctcc
      481 tcgccggctg gcatcaagat atgctctgca ccttttccta tctcacgagg tacgccaggc
      541 gccggccgat cgcggtgctt gtcagtcgga gcaaagacgg ggaggtcgcg gccggagtgc
      601 tttcgtggtt cggattcgtt ccgatccgcg ggtccagctc acgcggcggg gcagccgccc
      661 ttgcacagct ctgcgatcgt gtgaaaatag accgctcgat tgcggcgatc atctgcgacg
      721 ggcctcggcc tccggcacgc gtggcgaagc cggggatcat tacactctca cgcctcaccg
      781 gtcggccgat cttcctgctc cgatcctggt cgacgcgtca gcatattttt gagaaaagct
      841 ggccccgtct ggcactggtc cttcccttct cgcgcatcgt cgtgatgatc gaggggccgc
      901 tctcggttcc gccgagggca agcgatgaag agcaagaacg ccttcgtctc gaactccaag
      961 accgcctcaa ccgcctggct gaggcgtccg agaaatattt cattcgctga ccgtagaaac
     1021 ggcaaacccc aataatagta aagagttaga cagtttttga cttgaatgct atatttataa
     1081 gtcaaggggt atatcggggg gcccctctgg gagattgaag gaaatcatgg ctgataagac
     1141 tgtttggatc ctagatgact accaagagcg gtctgtttct caaaaggcgg ctcctcccac
     1201 gcgccttgag aagagtcccg ctgtggcttt cagtctttct ctgctcttct ggggaggcgg
     1261 ccagatctat aatcgacagt ggggattggg ttttctcttt atccttatta tggccaatgt
     1321 ctatgggacc ttggctatag caattctgtt ttgggatttc atcacatcct ctctcaagtc
     1381 agtctatatc acttctttcg aggtctttgc ggcttgcgga atattttatc tctttggact
     1441 gatcttctgg gccttcaatg cctttcatgc ctatcacaaa gcaacccagg cacgaaccga
     1501 tccatttcag ggaattgata accgcctgct gccgccgttg tgttcagctc tgatacccgg
     1561 ttggggacag ttcttaaacg gacagtcgaa gaaaggcgtc ttcttcctga tatttgccat
     1621 ggcgggccaa tttgtgctgg cagccttcct gctcattccc ctgttctggc cggccttgga
     1681 aaccgacatg gatcggctca tgctggaaaa agtcctggcg atcgctgtca tcctttcccc
     1741 tctgattctt ctgatgtggg gattcagcat ctatgatgcc gtgagggtct gtctggagcc
     1801 gctcaaaaag gaacctttac gaaaaaggtt cgagtacgcc atcaaccgga ttcgaataaa
     1861 agggtggaag caggcagttg tctcccgggc gaagccgatc ttcgtactgg gtctgttcct
     1921 cgtcctctca ctgacccttg gttattacta ctttcctcaa agagaatatg tcaccatgct
     1981 gcaaagcctc cggttccagc tatcggagca aaagatggtc ctcatcccag acctcatcgg
     2041 tcaatttgtt cagacgatct cacccgaagc cccgcggcac taacgggacc gtgaatgata
     2101 gacttatatt ctccacaaaa tgaagttgaa ttggctttat tgaaaagtat cctcgatgct
     2161 gaaagtatta attattttgt aaggaatgac aatttcggtt ccatgcaagt tggcccccag
     2221 attgatcttt acaacaaaaa gatgatagta gtgcaagata atcagtatga gagcgccaaa
     2281 gaacttgtgg cagattatct tgaaagaaca gagaagaaga tcgaagaacc cgagaaaaat
     2341 tattcggtgt ttgataaaat tcggatgact cttgaagtac tattattcgg atgggtaatg
     2401 ccaggtagaa aaaataaagg ggcatgatta acacgcatca atgacggcct cttcccatat
     2461 attcaaactt tcacctttgc atgtgcgatg cgggtcaggt tttgaaatat gattttcatc
     2521 tttaaaacct tacgcgtgtt gggccgcggc ggcgggaagg cgtaactgta gaagtaaagt
     2581 tttatcctgt ccgacagcgt cagaaaatgt gtgaacttct ttctggaatc cgcagg
//
LOCUS       DATMKH010000265         2461 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_124507,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000265 DATMKH010000000
VERSION     DATMKH010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124507"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VNV63_03185"
     CDS             <1..327
                     /locus_tag="VNV63_03185"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HXC61654.1"
                     /translation="AKENRSRKTVIETQKRRRFILITVACFFAVYFIFSFVFGDAGLL
                     SYFRMERTKGKLNHEIEDLRQKNAELKNQIEALRSDPQYIEQIAREQLGLVKDGEIIY
                     QFNDTP"
     gene            293..1255
                     /gene="era"
                     /locus_tag="VNV63_03190"
     CDS             293..1255
                     /gene="era"
                     /locus_tag="VNV63_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018886735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HXC61655.1"
                     /translation="MRLFTNSTTPLKERAPLVQTPALFRSGFIALVGRPNVGKSTLLN
                     RLIKQKLAIVSNKPQTTRTKILGIRHLPGAQLIFLDTPGIHKPKYALNRAMVKIALEA
                     ISDADLVLFLVEAKDPWGAGDRFILEQLKTVSSPVLLIINKIDLVRKGRLLPLLEESA
                     KLHPFVEMIPISALEGDNLDRLEEAILRYLPEGPPYFPEDTVTDQTLRFLAAEIIREK
                     ILHHTREELPYVVAVLLEDFKEEPKRKLTSITATIFVERDSQKAIVIGKGGAMLKKVG
                     QEAREEMESLLGTKVFLQLWVKVKEDWRQDEPILREMGYGEASR"
     gene            1252..>2461
                     /locus_tag="VNV63_03195"
     CDS             1252..>2461
                     /locus_tag="VNV63_03195"
                     /inference="COORDINATES: protein motif:HMM:NF015413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HXC61656.1"
                     /translation="MKYFLGEVFVSEVLGKPILDAAGEEIGRVKDFIVSPGEIFPKVA
                     FVVVTGPRGLIVIPWEQVAFFNKKFLSLNVLRGEIKSDTVKESDILISRDILDKQIVD
                     VNGAKVVRVNDLKLGNVKGYFCLVAADVGIRGLIRRLLGRQLKDQLGRIIHHRFAHNL
                     IGWNYLEPLKPRITRLTLSVPRRMLNKMHPADLATVISQMPAKDRIDFYESLDVKVAA
                     ETLHELEPTIQMEIVGRMDTDHTVDILELMPPDEAADVLGDLPAPKAQKLMALMNREE
                     AEEMKDLLAHTEDTAGGLMTTEFITFSPELTAGEMIEILRRESEDVETIYYVYVVDSS
                     ERPIGVITLRELIMAEPETHLSEIMNTRVKTVGPETHQSEVAKMMSKYNLIAIPVVDR
                     DQKMLGVITID"
BASE COUNT          597 a          607 c          705 g          552 t
ORIGIN      
        1 gcaaaagaaa accggtcgcg gaaaacagta atagagactc agaaacgccg tcgtttcatc
       61 ctgatcacgg tggcgtgttt ttttgccgtc tatttcattt tctcgtttgt ctttggagat
      121 gcgggccttc tgtcctattt cagaatggag aggacaaaag gaaaactcaa tcatgaaatc
      181 gaagatctcc gtcagaaaaa cgccgagctc aaaaatcaaa tcgaggcgct gcgatcggac
      241 cctcaatata tcgagcagat cgcaagagag cagctcggcc tggtcaaaga cggtgagatt
      301 atttaccaat tcaacgacac cccttaagga gcgcgcgcca ttggttcaaa cgcctgcgtt
      361 gttccggtcc ggtttcatcg cattagtagg tcggcccaac gtgggcaagt ccacgcttct
      421 caatcgcttg atcaaacaaa aattagcgat cgtctcgaat aaaccccaga ccacacgaac
      481 caagatcctg gggatccggc atctgccggg tgctcaattg atcttcctgg atacccccgg
      541 aattcataaa cccaaatatg cactgaaccg ggccatggtc aagatcgctc tggaggccat
      601 atccgatgcg gatcttgttc tttttctggt cgaggcgaag gatccgtggg gagccgggga
      661 ccgctttatt ctggagcagc tcaagacggt gtcgtcgcct gttctcctaa tcatcaacaa
      721 gattgacctg gtacggaagg gccgtctgct gccgcttctg gaggagagcg caaaactcca
      781 tccttttgta gagatgattc cgatctcggc cctggaaggg gacaatctcg ataggcttga
      841 ggaggcgatc cttcgttacc tcccggaggg cccgccttac tttccggagg atacggtcac
      901 ggatcagacc cttcgctttc tcgcggccga gatcattcgt gagaagatcc ttcatcatac
      961 tcgtgaagaa ctgccgtatg tggtggcggt cctgctggaa gatttcaaag aggagcccaa
     1021 gcgcaaattg acctccatca cggccaccat ctttgtagag cgggattctc aaaaggcgat
     1081 cgtgatcgga aaaggcggtg cgatgctaaa gaaggtcgga caggaggcgc gtgaggagat
     1141 ggagtccctt ctgggcacaa aggtgtttct ccaactttgg gtgaaggtga aggaggattg
     1201 gcggcaggac gagccgatcc tgcgtgagat ggggtacggt gaggcgtctc gatgaagtat
     1261 tttctcggag aggtctttgt gagcgaggtc ctggggaaac cgattctgga tgcggccggc
     1321 gaagagatcg gccgggtcaa ggactttatc gtctctccgg gagaaatctt tccgaaggtg
     1381 gcctttgtcg ttgtcacggg tccccgcggc ctgatagtga ttccatggga gcaggtggca
     1441 ttctttaata aaaaattttt gtcgctcaat gttcttcggg gagagataaa atccgatacg
     1501 gttaaggaaa gcgatatact gatctcaaga gacatcttgg ataagcagat cgtggatgtg
     1561 aatggagcca aggtggttcg agtgaacgat ctgaagctcg gaaacgtcaa aggctatttc
     1621 tgtctggtcg ccgccgatgt cgggatccgc ggcctgatcc ggcgcttgct cggtcgccag
     1681 ctcaaagacc agctggggcg catcattcac caccgctttg cgcataacct gatcgggtgg
     1741 aattatctgg agcctttaaa acccaggatt acacggctca cgttgagcgt cccgcggaga
     1801 atgttgaaca agatgcatcc ggccgatctg gctacggtga tcagtcagat gcctgccaaa
     1861 gatcgcatag acttctatga atcgttggac gtcaaggtag cggccgagac cctgcatgaa
     1921 ctcgagccga cgatccaaat ggaaatcgtt ggccggatgg acacggatca taccgtggat
     1981 atcttggagc ttatgccgcc cgatgaggcg gcggacgtac tcggagatct ccctgcgccc
     2041 aaggcacaga agctcatggc gctcatgaat cgcgaggagg cggaagagat gaaggatctc
     2101 cttgcccata ccgaagacac ggccgggggt ctcatgacga ccgaattcat taccttctcg
     2161 cctgaactca cggccggcga gatgatcgaa atactccgga gagagtcgga ggacgtggag
     2221 acaatctatt atgtgtatgt ggtggactcc agtgagcgtc ccataggagt cattactctt
     2281 cgcgaactca tcatggcgga gcctgagacg catctttccg agatcatgaa cacgcgcgtc
     2341 aagaccgttg ggcctgagac acatcaaagc gaagtcgcca agatgatgtc caagtacaat
     2401 ctgatcgcca taccggtggt ggaccgggat cagaagatgt tgggcgtcat tacgattgat
     2461 g
//
LOCUS       DATMKH010000266         1046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3007861, whole genome shotgun sequence.
ACCESSION   DATMKH010000266 DATMKH010000000
VERSION     DATMKH010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3007861"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..>1046)
                     /locus_tag="VNV63_03200"
     CDS             complement(157..>1046)
                     /locus_tag="VNV63_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61657.1"
                     /translation="PSKDGKKLFVVGVQRRAELVRYEAKSGHFVPYLGGISAGDVDFS
                     RDGQWVTYVLYPEGTLWRSRLDGSERLQLTSPPMRTALAHWSPDGQQIAFSGSHAGKW
                     KVFLISRDGGNPQPVTTGDEQETDPTWSADGTKLAFGINSVAGSAQTFIQVLDVKTHE
                     LSHLSGSQGLFGPRWSPNGHSIVALSYDNTKLLLFDTASQQWRHLIAMPALIGYFAWS
                     TDGSYIYFDTLSTPDPGYFRLRVSDSKLDRLIDLKGIRTFPEEFGPGSWTGLGPGEIP
                     LFPRDISTQEIYALDLDLP"
BASE COUNT          253 a          261 c          321 g          211 t
ORIGIN      
        1 tttgaaattg ttagagattt aggggctagg gatacccgca accgccagcc ggatcggccc
       61 tcctactgca acttcgcgta ctcggctttg gcttgtttga ggatcgggat gtcagggtca
      121 gcgtctttct aagggaccag aaaaatcctg gagaaactaa ggaagatcaa ggtcgagggc
      181 gtaaatctcc tgagtgctga tatcgcgggg aaagagcggg atttccccgg ggcccaggcc
      241 ggtccaagaa ccggggccga actcctcggg gaacgtacgg atacctttga gatcaatgag
      301 gcggtcgagt ttggaatcgc tgacgcgaag ccgaaagtat cccggatccg gtgtgctcaa
      361 agtgtcgaag taaatgtaag agccatctgt agaccaggca aaatacccga tcaaggccgg
      421 catggcaatc aaatggcgcc actgctggga ggccgtgtcg aagagcaata gtttggtgtt
      481 gtcgtaggaa agagcgacga tggaatggcc attcggggac caacgcggac cgaagagtcc
      541 ctgggaaccg gacaggtgag aaagctcatg ggtcttgacg tccagcactt gaatgaaggt
      601 ctgagcagac ccggctacgc tgtttatccc aaaggccaac ttggtgccat ccgcggacca
      661 agtcgggtcc gtttcctgtt catcgcctgt tgtgactggc tggggatttc caccatctct
      721 ggagatgaga aaaaccttcc acttgcccgc atgggatcca gaaaaggcga tttgttgacc
      781 gtcgggagac cagtgggcta gagcggttcg catcggagga gaggtaagct ggagacgctc
      841 gctaccatcg agcctgctac gccagagagt cccttccgga tagagaacat aggtaaccca
      901 ttgtccgtct ctggagaaat cgacatcgcc agcggaaatt ccgccgagat aaggaacaaa
      961 gtggccggat ttcgcttcgt aacggaccag ttcggcgcgc cgctggacgc cgacaacgaa
     1021 taacttcttg ccgtccttgc tgggga
//
LOCUS       DATMKH010000267         1413 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1405046, whole genome shotgun sequence.
ACCESSION   DATMKH010000267 DATMKH010000000
VERSION     DATMKH010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1413
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1405046"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /gene="panB"
                     /locus_tag="VNV63_03205"
     CDS             complement(<1..516)
                     /gene="panB"
                     /locus_tag="VNV63_03205"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012610908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HXC61658.1"
                     /translation="MARITIPDILRKKEEGKKIIMLTAYDYPFAKIVDEAGMDIVLVG
                     DSLGMVVQGRDTTLSVTMEEMLYHTRMVTSAVKNALVIADMPFLSNQMGVSETIKNAG
                     RFLKEARAAGVKLEGGVMVADQIRALVRAEIPVMAHVGMTPQSIHRFGGYKVQGKEKR
                     TALKILEDATAV"
     gene            complement(529..1407)
                     /locus_tag="VNV63_03210"
     CDS             complement(529..1407)
                     /locus_tag="VNV63_03210"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HXC61659.1"
                     /translation="MKRLRESLEKGEFVITAELEPPKGTDLTNVLANAKELVGKVHGV
                     NVTDNQTAAMRMCPLVVCHRLLELGLDTIMQVTVRDRNRLALQSDLLGAHAIGIRNVL
                     TLTGDPITVGDHKEAKTVFDIESTQILQIIDGLNHGKDMAGNDLHGATDLWGGAAASP
                     DAPSLDIELSKFEKKVNCGAKFFQTQGVYDPDSFKRFMDKISKYNVKVLAGIILLKSA
                     AMAQHMNKNIPGIKVPDAMIQQLEKAGKDHALEAGIEIAAKTIQEVRRYSSGVHIMAV
                     GARNKVPEIIRQAGIS"
BASE COUNT          300 a          416 c          328 g          369 t
ORIGIN      
        1 aaccgctgtg gcgtcctcca atatctttag agcagttcgc ttttctttcc cttggacctt
       61 gtagcccccg aatctgtgga tggattgagg tgtcatcccg acatgggcca tcacgggaat
      121 ctctgcccgg accagggccc ggatctgatc tgcaaccata accccgccct ccaatttcac
      181 acccgctgcc cgggcctctt taagaaaacg acccgcattc ttaatcgtct cggatacacc
      241 catctggttg gagagaaaag gcatatcggc aatcaccagg gcgttcttca ccgcgctcgt
      301 aaccatgcgt gtatggtaaa gcatctcttc catcgtgacc gagagggtcg tgtcacgacc
      361 ttgaaccacc attccaagag aatcacctac caggacgata tccatccccg cttcatccac
      421 gatcttggca aatggatagt cataagcggt aagcattatg attttcttgc cttcttcttt
      481 ttttctcaga atgtctggaa tcgtgatcct ggccatcgcc gccttctttt atgaaatgcc
      541 tgcttggcga ataatctcgg ggaccttgtt tcgtgcccca accgccatga tgtggacgcc
      601 gctagaataa cgacgaacct cctggatcgt tttggccgca atctcgatgc cggcctctaa
      661 ggcatgatcc ttccccgcct tctcgagttg ctggatcatc gcgtccggca ctttgatacc
      721 ggggatgttt ttattcatgt gttgggccat tgcggccgat tttagcagta tgattccggc
      781 caggaccttg acattatatt tagaaatctt atccataaag cgtttgaagg agtcggggtc
      841 gtacacgcct tgcgtctgaa aaaacttggc gccgcagttt actttcttct cgaacttgga
      901 cagttcgata tcgagagaag gcgcatccgg actcgccgcc gcaccgcccc aaaggtccgt
      961 ggctccatga aggtcattcc ccgccatgtc ttttccatgg ttgagcccgt cgataatctg
     1021 gaggatctgc gtcgattcga tgtcaaagac cgtcttggcc tctttatgat cgcccaccgt
     1081 gatcggatcg ccggtcaatg ttaagacatt ccggataccg atcgcatgag cgcccaggag
     1141 atctgactgg agcgccagac gattccgatc ccgcaccgtg acctgcatga tcgtatccaa
     1201 accaagttcc aataaacgat gacagaccac aagcgggcac atccgcatcg cggcggtctg
     1261 attgtccgtg acattgaccc cgtgtacctt cccgaccagt tcctttgcgt ttgctagaac
     1321 attagtcaga tccgttccct ttggcggttc cagctccgcc gtgatcacaa actccccttt
     1381 ctccaatgac tcgcgtagtc gtttcattta aca
//
LOCUS       DATMKH010000268         1525 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4293222, whole genome shotgun sequence.
ACCESSION   DATMKH010000268 DATMKH010000000
VERSION     DATMKH010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4293222"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="VNV63_03215"
     CDS             <1..364
                     /locus_tag="VNV63_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61660.1"
                     /translation="DFQNLSKSNGKDISWDADGVRYISDAKNLRITWVLDPDFLPSMD
                     GHPTHVFKGKFSIFGSEIVPHQPLPTDWLSKYGFSATGDQVPRYYQLEQNGWKINIIV
                     DRDRNPEAVTLQHPLNPD"
     gene            complement(381..995)
                     /gene="msrA"
                     /locus_tag="VNV63_03220"
     CDS             complement(381..995)
                     /gene="msrA"
                     /locus_tag="VNV63_03220"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HXC61661.1"
                     /translation="MKIIYALLILACFTTSDRVFAQMNDSKSSPKLESAVFGGGCFWC
                     LDAQFKLINGVKSVLSGYAGGTTKNPTYEQVCSETTGHAEVIQVEFDPAVVSYEDLLR
                     KFFRAHDPTTLNRQGPDVGTSYRSIILYKDENQKAIAEKVKAEAQKLWPDPIVTEIAP
                     LNAFYTAEDYHQDYYAKNPDQGYCRVVVAPKVKKFEKLLKEEKP"
     gene            complement(1038..>1525)
                     /locus_tag="VNV63_03225"
     CDS             complement(1038..>1525)
                     /locus_tag="VNV63_03225"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HXC61662.1"
                     /translation="DEKDLPYDRILVPIDFSEHSKRTVSYATRTATRHNSTIYLLHVF
                     QIPDYVVTPYARRRQNSAEVQSHLVAAEQEARETLEAFAQELLNRGIKVQPYLRVGYP
                     FDEIVLMANHFKVDLIIIGSHGRSGISRLLVGSTAARVVEHALCPVLVVKGPRPGAGS
                     E"
BASE COUNT          351 a          403 c          391 g          380 t
ORIGIN      
        1 ggactttcag aacctgtcta aatcgaatgg aaaggacatt tcttgggatg cggatggagt
       61 ccgatacatt tcggatgcca aaaatttgcg cattacctgg gtgcttgatc ccgactttct
      121 cccatccatg gacggtcatc cgacccacgt ctttaagggg aaattttcca tcttcggcag
      181 cgaaatcgtt ccccaccagc ccttaccgac tgactggtta tcgaagtatg gattttcggc
      241 gactggtgat caggtgccca gatattacca actggagcaa aatggatgga aaataaatat
      301 cattgtcgat cgggatcgga atccggaagc ggttactctt caacatccct taaaccccga
      361 ttgaaaactc tcgaaaaggc ttacggcttc tcctctttca ggagtttttc gaattttttg
      421 actttcggcg ctacgaccac tcggcaatat ccctggtctg ggtttttcgc ataataatcc
      481 tgatgatagt cttccgccgt gtagaaagcg ttgagaggcg caatctcggt cacgattgga
      541 tcgggccaca gtttttgcgc ttcggccttc accttttcag cgatggcctt ttgattctcg
      601 tccttgtaca ggatgatcga cctgtacgaa gtgcccacat ccggaccctg tcgattcaag
      661 gtcgtcgggt catgagcgcg aaagaatttc cgcagcaaat cctcgtagct gacgacggcg
      721 ggatcgaatt caacctgaat cacttcagcg tgcccggttg tttcgctaca cacctgttcg
      781 taagtcgggt tcttggtcgt tcctccggca taccccgaga gcacggactt gaccccgttg
      841 atcagtttaa attgagcatc aagacaccag aaacagccgc cgccgaaaac agccgactcc
      901 agtttaggcg aggattttga atcgttcatt tgtgcgaaaa ctcggtccga tgtggtgaag
      961 caagcaagaa tcaatagcgc gtaaatgatc ttcatatgac cggtagctct gaccaataga
     1021 ggtctcagaa ggccggttta ttccgacccc gccccaggtc gcggtccctt cacgacgagc
     1081 accgggcaca gcgcgtgttc gacgacgcgc gcggccgtgc tgccaaccag caaccgcgaa
     1141 attccgctgc ggccgtgcga tccaatgatg atcagatcga ccttaaaatg gtttgccatc
     1201 aggacaatct cgtcgaacgg atatcccacc cgcaggtaag gctgaacttt gattcccctg
     1261 tttagaagct cttgggcgaa cgcttcaagg gtctccctgg cctcttgctc ggcggcaacg
     1321 aggtgcgatt ggacctccgc ggagttctgc ctgcgtcgcg catacggagt gaccacatag
     1381 tcagggatct ggaagacgtg tagaagatag atggtcgagt tgtgcctggt cgccgttctc
     1441 gtggcgtacg agacggtcct tttggaatgc tcagaaaaat cgatcggaac cagaattcta
     1501 tcgtaaggaa ggtctttttc atccg
//
LOCUS       DATMKH010000269         1456 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1088011, whole genome shotgun sequence.
ACCESSION   DATMKH010000269 DATMKH010000000
VERSION     DATMKH010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1088011"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="VNV63_03230"
     CDS             complement(<1..684)
                     /locus_tag="VNV63_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015080284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HXC61663.1"
                     /translation="MQEYVPGLAGIPAARSKISYLDGQKGILEYRGIRIDILAEKSSF
                     VETAYLLLFEKLPTRPALEKFSQDIIAHRKIKYRITDLMKYLPEHGHPMDALQAAVAA
                     LGMFYPAKDVLNPQVQYDSVVRLVAKLPTIIAAFVRLRRGDDHILPREDLGHAANFLY
                     MLTGKVPEPMMAEILDVCLILHAEHTMNASTFSGRVTASTLADAYTVVSSAIGTLMGP
                     LHGGASEEVV"
     gene            complement(696..1178)
                     /locus_tag="VNV63_03235"
     CDS             complement(696..1178)
                     /locus_tag="VNV63_03235"
                     /inference="COORDINATES: protein motif:HMM:NF015126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HXC61664.1"
                     /translation="MKKKVKKKAKKPVKKKTAAKKTIKKKTVKKAVAKKHPVKKKRAA
                     KKPTPKKAMMKRAAPKPSARPMAKAPSVEAPAGGKPVGKVIHYYTLLSVAIVELIQGT
                     LRVGDTIHLKGHTTNFKQTVESMEIEHQRVTEAAPGQSFGIKVREHARENDIVYKVAS
                     "
     gene            complement(1271..>1456)
                     /locus_tag="VNV63_03240"
     CDS             complement(1271..>1456)
                     /locus_tag="VNV63_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXC61665.1"
                     /translation="FHVVSSPDVDLISKMADSEDCLMEPAKNGGEILGYYCIVADPDG
                     NLVEFSYGQAKAPCDRE"
BASE COUNT          295 a          377 c          388 g          396 t
ORIGIN      
        1 taccacttcc tcgctggccc cgccgtgaag cgggcccatc agggttccga tggctgaaga
       61 aactacggta taggcgtccg ccagggtaga ggcggttacc cggccgctga aggtggaggc
      121 attcatggta tgttccgcat ggagaatcag gcagacatcc agtatctctg ccatcatggg
      181 ttcagggacc ttcccggtga gcatgtagag aaaattggcc gcgtgcccca gatcctcacg
      241 gggcaggatg tgatcgtcgc ctcgcctgag acgtacaaag gctgcgatga tcgtgggcag
      301 tttggcgacg agtcgaacga ctgagtcata ctggacctga gggtttaaaa catccttggc
      361 cggataaaac atgccgagag cagccacggc cgcctgtaac gcatccatcg ggtgaccgtg
      421 ttccggcaga tatttcatga gatccgtaat ccggtacttg atcttccgat gggcgatgat
      481 gtcctggcta aacttctcaa gagcagggcg tgtgggtagt ttttcaaata gaagaaggta
      541 agcggtctcc acaaagctgc tcttttcagc caggatgtca atgcggattc ctcgatattc
      601 cagaatgccc ttctggccgt cgagataact gattttggac cgggcggccg ggatgccggc
      661 caggcccggg acatattcct gcatagacgg tccttttaac tggctacctt gtagacgata
      721 tcattctcgc gtgcatgttc acggaccttg attccgaaac tctggccggg tgcggcctct
      781 gtgacccttt gatgttcgat ctccatagat tccaccgtct gcttaaagtt ggtcgtgtga
      841 ccttttaggt gtatcgtatc cccgactcga agcgtgccct gaatcaactc cacgatcgct
      901 acgctcagaa gggtatagta atggatcact tttccgaccg gctttcctcc agccggcgct
      961 tccacggagg gagcttttgc catcggtcgt gctgaaggtt tcggggcggc gcgtttcatc
     1021 atagcttttt ttggtgttgg tttcttggcc gctctcttct tcttgaccgg gtgctttttg
     1081 gcaacagcct tcttaacggt ttttttcttg atggttttct ttgcggctgt tttcttcttt
     1141 acaggttttt tggctttctt cttcactttc tttttcatgg cggctccttt cgagggataa
     1201 cgacccctgg tgattaagat ctaaaagcgt gtgatcctat tgtagtcagc cgaaaataaa
     1261 aaagcaatgg ctactctcgg tcgcacggcg ctttggcctg accgtatgaa aactcaacga
     1321 ggtttccatc cgggtcggcc acaatacagt agtagcccag gatctctcct ccgttcttgg
     1381 ctggttccat caaacagtcc tcggaatcgg ccatcttaga aattaggtct acatcaggac
     1441 tgctcacaac atggaa
//
LOCUS       DATMKH010000270         1877 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3008814, whole genome shotgun sequence.
ACCESSION   DATMKH010000270 DATMKH010000000
VERSION     DATMKH010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3008814"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1012)
                     /locus_tag="VNV63_03245"
     CDS             complement(<1..1012)
                     /locus_tag="VNV63_03245"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HXC61666.1"
                     /translation="MRRNIINVGLVMSLVILGATAGVVYKNTNELVHTSDWVSHKQEI
                     IEELGSTLISLTDAETAQQGYIRTGGEQQMEMYNAAIQVIHERLNRLRTLTLDDRKQQ
                     QRLNAIEPLVTQKLAVLNDSIQLWKDKPSDAESQAFLNSQGRTLMEDIRGIIAELKIE
                     ENELLKQRREDSKTKTQEAILILFMGNILSIALLALVFYRLNREIAEHKHAQEVLSSE
                     RAGIFSEDTATLFPIHELPLAQITGGEEANGDQREDESRKFNGALEYQRIEYTAADRD
                     IILLGEMGDLLRTCRAPEEAYNVLAHCLGKLFPAASGSLCVLTAPQNLVETAVVWGDS
                     EPD"
     gene            1203..>1877
                     /locus_tag="VNV63_03250"
     CDS             1203..>1877
                     /locus_tag="VNV63_03250"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF018242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HXC61667.1"
                     /translation="MVEADKKKKKLSPEDQVCVLISGGLDSCVLAAELSHQYLTLFPV
                     YVKGGLFWEPVELFWLQKFLSSLKTRTIQPLTILRMPLDDLYQNHWSLTGKQIPAYHS
                     DDRKVYLPGRNILLLAKTAVYCEMHGIQNIALGPLKANPFPDSTPGFFKTMQNALDLG
                     LNARLNILTPLAKLSKADVLKKGAGLRLDFTFSCLSPQGYHHCGRCNKCAERILSFRN
                     AGIEDKT"
BASE COUNT          442 a          525 c          447 g          463 t
ORIGIN      
        1 gatccggctc ggaatcgccc caaacaacgg cggtctcaac caggttttgc ggggccgtaa
       61 gcacacacaa cgaccccgac gcggccggaa ataatttccc taggcaatgg gccaagacat
      121 tgtaggcttc ctcaggggca cggcaagttc ggagcaaatc gcccatctcc ccgagcagga
      181 tgatatctct atcggcagct gtgtattcaa tccgctggta ttcaagtgct ccgttaaact
      241 ttctgctctc atcctccctt tgatccccat tggcctcctc accgccggtg atctgtgcca
      301 gcggtaattc atggataggg aaaagcgttg ccgtatcctc ggaaaaaatg ccggctcttt
      361 cagacgatag cacctcttgt gcatgtttat gctctgcgat ctcacgattg aggcggtaga
      421 agaccaaggc cagaagagca atgctcaaaa tgttccccat gaagaggata agaatcgctt
      481 cttgggtctt ggttttagaa tcctcacggc gttgcttcaa taactcgttt tcctctattt
      541 tcaactcggc aatgattccc cggatgtcct ccataagcgt tcgtccctga ctgttcagga
      601 aggcctgcga ttcggcgtcc gacggcttgt ctttccataa ttggatggag tcgttaagca
      661 cggccagctt ttgagtgacg agcggttcga tggcgttcag tctctgttgc tgcttgcgat
      721 catctaaagt cagtgtccgc aagcgattta gtctctcatg gattacctga atcgcagcat
      781 tgtacatctc catctgctgc tctcctcctg tgcggatata gccctgctgc gccgtctcgg
      841 catccgtcag agaaattagg gtgcttccga gttcttcgat aatctcttgt ttgtgagaaa
      901 cccaatcaga ggtatgaaca agctcgtttg tgtttttata gacaacgccg gcggtggcac
      961 ccaaaatcac caacgacatg acaagtccga cattgataat atttcttctc atacatcatt
     1021 ccctacgatt acggcactct cactaaatca ggcgcctccg gtacagagat gctgcaggca
     1081 cttcaaacac cgctttcaag gaattttaat ctaccatatc ctctaaagac ggtcaatggt
     1141 tccaggtaaa gcaggtttct tctcttgatt ttataaaatc ggcttgctag gataccggcg
     1201 acatggtgga ggctgacaag aaaaagaaga aactctcacc ggaagatcag gtttgcgtct
     1261 tgatcagcgg cggtctggac agctgcgtcc tggcggctga actaagccac caatatctaa
     1321 cgctctttcc ggtctatgta aagggcggcc tcttctggga accggtcgag ctgttctggc
     1381 tccagaaatt cttgtcgagc cttaaaacaa gaacgattca acccctgaca atccttcgca
     1441 tgccgctcga tgacctctat cagaaccact ggagcctgac cggcaagcag atacccgctt
     1501 atcactccga tgatcgaaag gtgtatcttc ccggcaggaa catactgctc ctggccaaga
     1561 ccgccgtcta ctgcgagatg catgggattc agaacatcgc tctggggccg ctcaaggcca
     1621 acccgtttcc ggacagtaca cccggattct ttaaaacgat gcagaacgcg ttggaccttg
     1681 gattgaacgc ccgactaaac atcctgaccc cgctggccaa gctctctaag gccgatgtcc
     1741 tcaaaaaagg cgcgggcctg cggctcgatt tcactttctc ctgcctcagt ccgcagggat
     1801 atcatcactg cggccgatgc aacaaatgcg ccgagcgcat tctctccttc cgcaacgcgg
     1861 gaatcgaaga caagacg
//
LOCUS       DATMKH010000271         1045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_128879,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000271 DATMKH010000000
VERSION     DATMKH010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128879"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VNV63_03255"
     CDS             <1..265
                     /locus_tag="VNV63_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007957389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HXC61668.1"
                     /translation="GGMITIYLASDLAGVRRFLENTHLFSLAESLGGVESLIEHPGIM
                     THGSIPPEQRRALGIDDTLVRLSVGVEDLEDLRTDLAEALDKV"
     gene            complement(389..760)
                     /locus_tag="VNV63_03260"
     CDS             complement(389..760)
                     /locus_tag="VNV63_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HXC61669.1"
                     /translation="MKKSPGLILLSGSLFAFSLFGGVVQAETLIPDTEAAQHVGQAVA
                     VEGTITRVFKSRNGNIFLNFGGAYPNITFVVWIPQDAPEAADPRLTNLQGKKVKIVGT
                     IQLYRDKPETKVSTKEQIVAE"
BASE COUNT          223 a          287 c          267 g          268 t
ORIGIN      
        1 cgggggcatg atcacgatct acctcgcttc cgatttggcc ggtgtcaggc gtttcctgga
       61 gaacacgcat ctcttctcct tagccgaaag tttgggtggc gttgaaagcc tgatcgaaca
      121 ccccggcatt atgacccatg gttcgatccc tcccgaacaa agacgtgcgc ttggcatcga
      181 cgacacgctg gtgcgcttat ctgtaggcgt tgaagacctg gaagatctga gaaccgatct
      241 cgcagaggcg ctcgataaag tgtagtagcc gaatgccctt gtttggcgtt cggcgtttcg
      301 tcctcgagtc ctggagagcg gagtgatgga gtgttggagg gctgcggcct tctcaggatt
      361 ggaccccgct caacgggttg gggaatggtt actccgccac aatttgttcc ttggtgctga
      421 cctttgtctc cggtttatcg cggtataact gaatcgtacc tacgatcttc acctttttac
      481 cctgcaaatt ggtcaacctc ggatccgctg cctcgggtgc atcttgtggg atccaaacca
      541 cgaaagtgat gtttggatat gctccgccga aattgaggaa aatgttccca ttcctgcttt
      601 tgaagaccct tgtaattgtt ccttccactg ccacagcctg cccaacatgc tgagccgctt
      661 ccgtgtcggg aatcagcgtc tcagcctgaa ccacgcctcc aaaaaggcta aaagcaaaca
      721 acgaaccaga aagcagaatt aaaccaggtg atttcttcac acgaatcttg cagcgttcac
      781 cgtcttcggc ttcttcgcta gttagaactt acggcagttt cctttttggt ctggcgcatc
      841 gcgctcgtca gtccacccgc gcaagcttct ccgggacatg ccggatagga ggtttacaat
      901 acggagagat cacaaaggac accaagacgg aaataagaat cagattcata gcttcagtct
      961 ttgtggcctt ggtgtccttt ccgttctgtg gccctcggtt tgtttccccc gaaatcgacc
     1021 tgagcccgat tcaagccggc taccg
//
LOCUS       DATMKH010000272         2452 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1091811, whole genome shotgun sequence.
ACCESSION   DATMKH010000272 DATMKH010000000
VERSION     DATMKH010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1091811"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="VNV63_03265"
     CDS             complement(<1..390)
                     /locus_tag="VNV63_03265"
                     /inference="COORDINATES: protein motif:HMM:NF016553.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HXC61670.1"
                     /translation="MKFQDLVDYFERLEATSKRLEMFEILADLFRVAKSEEIAEIVYL
                     SQGELLPSFHGLEIGMSEKLLIRAIALVTEKPAEEIGREFKKSGDLGKTAELFVKGKG
                     QGLSILEVYRTLLTVAKTAGEGSVEKKI"
     gene            complement(630..>2452)
                     /locus_tag="VNV63_03270"
     CDS             complement(630..>2452)
                     /locus_tag="VNV63_03270"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HXC61671.1"
                     /translation="AELYERHYFQENVRTVASREASGSEPRERIESHALQSGRLYLQA
                     GQVQKAASIFAMGGFVKEAADAYLNAKQPEKAAELYQSVKEFSKAVELYSQLGLHKKA
                     AFLQAELFSEKGDYADAARMFEKAEEFGQAGDLYEKANNLPKAGEMFMKGKEFIRASE
                     VFLSVGDERKAAMAFEQAKQLKEAASLYQKLGEYATAAHLLEESGDFYQAGVLYHKLG
                     RLNETIESLQRVESQSQNYYHASVMLGKVLVEQGMLDAAKERYKKLLTKMEMGSETLE
                     PYYNLAVIYEHDKDLSNALKIYDKILAEDYNYKDVRPRVEQLKKLVGLERQKMEKMKS
                     GGSQGTVSPTSVGRYRRIRKVGQGGMGVVYQAEDTVLHRNVAYKMLPQSLKDHPKVLA
                     NFLQEARVAAAINHPNIVTIYDTGRDEEGVFITMELVEGVSLKELLDKANKLSVPDFL
                     DIAKQICEGLDFAHSRNVIHRDIKPGNIMVNRDKVVKIMDFGLAKVLTDSMLDKTSVK
                     GTPLYMAPEQILGEKVDHRSDIYSLGCTFYWMATGRPPFVEGDVYYHHLHTIPTPPCT
                     LNPALPEQLGRLIMKCLAKDKAKRYQRVKEVLEELETIKI"
BASE COUNT          537 a          679 c          565 g          671 t
ORIGIN      
        1 aatctttttc tcgacgctcc cttcgcccgc cgttttggca acagtcaaga gcgtccggta
       61 aacctccagt atcgaaagcc cctggccttt gccttttaca aaaagctccg ctgttttgcc
      121 gagatcgcca ctctttttaa attcccggcc gatctcctct gcaggttttt cggtcactaa
      181 tgcaatggcc cggatcagaa gtttctcgga catgccgatt tcaagcccat ggaaagatgg
      241 aagcagttcg ccttggctca ggtaaacgat ctcggcgatt tcttcagact tggctacgcg
      301 aaacagatcc gccaggatct cgaacatctc aaggcgcttg ctggttgctt ccaaccgttc
      361 aaaataatct acaagatcct gaaatttcat ggcaccactc tattcttctc aaaaggccgt
      421 gtcaaggcaa ggaacaaccc gctctatcat tttacacttg acaattttta acgatggtta
      481 tttaatagtg gcctaaatcc taggaggttc tatgaagatg gctcttacaa gtacagattg
      541 gatggagctg ttcaaaatgc tcgttatcag tgccctgttg ctaggttcga tcttcgcggt
      601 tgtttttctt ttgaatatgt ttttcagcgc taaattttaa ttgtctccaa ctcttccaga
      661 acttctttaa cccgctgata gcgtttggcc ttatctttcg caagacattt cattatcagc
      721 cggccgagtt gttcaggaag ggctgggttc aatgtgcaag gcggggtggg aatggtatgg
      781 agatggtggt agtagacgtc tccttccaca aagggcggac ggcctgtggc catccagtag
      841 aaggtacaac cgagagagta gatatcggac cggtggtcca ccttttctcc caggatctgc
      901 tccggggcca tataaagcgg cgtgcctttc acggaggtct tatccagcat ggaatcggtt
      961 aggaccttgg ccagcccgaa atccatgatc ttgaccactt tatcccggtt caccatgatg
     1021 ttccccggct tgatgtctcg gtgaatgacg ttccggctgt gggcaaagtc taagccttca
     1081 cagatctgtt ttgcgatatc cagaaaatcc ggaactgaaa gtttgttggc cttgtccaac
     1141 agctccttca agctgacccc ttccactaat tccatggtga taaaaacccc ttcttcatcc
     1201 cgaccggtgt catagatcgt tacgatattg gggtgattga tagcagcagc caccctcgct
     1261 tcctgcagaa aatttgcaag aaccttgggg tgatccttta ggctctgggg aagcatcttg
     1321 taggctacgt tgcggtgaag aaccgtgtcc tccgcttgat ataccacccc cattcctccc
     1381 tgacccacct tgcggatccg acgatagcgt ccgacggaag tgggactgac cgtaccctgg
     1441 ctgccgccgg atttcatctt ctccattttt tgacgttcca gaccgaccag ctttttgagt
     1501 tgttcgactc gcgggcgaac atccttgtaa ttgtaatctt ctgcaaggat cttatcatag
     1561 atcttgaggg cgtttgataa gtccttatcg tgttcgtaga tcacggcgag attgtaataa
     1621 ggctccaagg tctccgatcc catctccatt ttcgtaagga gctttttgta ccgttccttc
     1681 gccgcatcca gcataccctg ttctaccaac accttcccca gcatcaccga tgcatggtag
     1741 tagttctgag actgggactc cacccgttga agggactcaa tcgtctcatt caaccgaccc
     1801 agcttatgat agagcacgcc ggcctgatag aagtctcctg actcttccaa aagatgggcc
     1861 gccgtggcat attcgcctag cttctggtaa agagacgccg cttctttcaa ttgcttcgcc
     1921 tgctcaaacg ccatggccgc ctttcgctca tcgcccaccg aaagaaagac ctcgctggcc
     1981 cgtatgaact ctttcccctt catgaacatc tcaccggcct ttggcaggtt gttcgctttt
     2041 tcatacaggt caccggcctg gccgaactct tcagccttct caaacatgcg ggccgcatcg
     2101 gcataatccc ctttctctga gaacaactcg gcctgaagga aagcagcttt cttgtgaagg
     2161 cccaattgac tgtacagctc gaccgctttg gaaaattctt ttacagactg gtacagttcc
     2221 gccgccttct cgggctgttt cgcgttgaga taggcatccg ccgcttcttt cacgaagcct
     2281 cccatggcaa aaatgctggc agctttctga acctgccccg cttgaagata aagccgtccg
     2341 ctctgtagag cgtgcgattc gattcgttcc cgaggctctg aacccgaagc ctccctggag
     2401 gcaaccgtcc ggacgttctc ctgaaaatag tggcgctcat aaagttcagc tg
//
LOCUS       DATMKH010000273         1622 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3013825, whole genome shotgun sequence.
ACCESSION   DATMKH010000273 DATMKH010000000
VERSION     DATMKH010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3013825"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="VNV63_03275"
     CDS             <1..480
                     /locus_tag="VNV63_03275"
                     /inference="COORDINATES: protein motif:HMM:NF028441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HXC61672.1"
                     /translation="RFTNPPETIKLQLTDSGSVLSDEKAAGQQILSGSRLSEPVRGTD
                     ISYEDLSLRFIYWKNARDEGEQRVRAITCSIVLVQPSARNTEYGSVRLWIAKDRGALI
                     KAEGYNWQGKPIKRFEVISGQQIEGRTIFKQIRIERLDPQSGKVVSRTYLELDPTDG"
     gene            complement(491..1258)
                     /locus_tag="VNV63_03280"
     CDS             complement(491..1258)
                     /locus_tag="VNV63_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61673.1"
                     /translation="MASVLFAQTVTDSAARFTDNAMKLRESILFKVEPKDLDSNPLQG
                     PAEKFPWHAQIVTTVFWIGETPSGNNPVPNRSSAWDSNWARNFGGSDSPAPDERKGFI
                     PARFVPRQNPFYLALPFNDVSGGRTKPESYKVIPWFRETFEKSGQSILKGRWVAIRRG
                     NRICYAQWEDVGPFRSDHWEYVFGSDRPRPNLNRGAGLDVSPAIRDYLKLGNTDLTDW
                     RFVDFQEVPPGPWALYGDNNTFVIERRKREDAFAKRQ"
BASE COUNT          415 a          447 c          419 g          341 t
ORIGIN      
        1 cggttcacta acccgccgga aaccatcaaa ctgcagctca ccgatagtgg ctccgttctg
       61 tcagacgaaa aagccgcggg tcaacagatc ttgtccggtt cgagattaag cgaacccgtg
      121 cgcggcacgg acattagcta cgaagatctc tcgttacgct ttatctattg gaagaatgcc
      181 agggacgaag gggaacaacg cgtgcgggcg atcacctgtt ccatcgttct agtgcaaccc
      241 tctgctcgca acaccgagta tggctctgtc cgactgtgga tcgccaagga tcggggagcg
      301 ctgataaaag ccgaagggta caattggcaa ggcaagccga tcaaacgctt tgaggtgatt
      361 tctggccagc agatcgaagg aaggaccatc ttcaaacaga tccgcattga gcggctcgat
      421 ccacagagcg gcaaggtggt ctcacggacc tacctggaac tcgacccgac agatgggtag
      481 gggagttcgc ttactgtctc ttcgcgaaag cgtcttctcg tttgcgacgt tcgatgacaa
      541 acgtattgtt gtcgccgtac agcgcccatg gaccgggtgg aacttcctgg aaatcgacaa
      601 aacgccaatc ggtcagatcg gtattcccca acttcaaata atcccggatc gcaggcgaga
      661 cgtcgagacc agctccacga ttcaggttcg gacgcggccg gtcgctgccg aaaacgtact
      721 cccaatgatc gctgcgaaac gggccgacat cttcccattg ggcgtagcaa atccgatttc
      781 ctcttcggat cgccacccat cggcctttta gaatgctttg accggatttt tcaaacgtct
      841 cccggaacca gggaatgacc ttgtacgact caggtttcgt ccgacctcca ctgacatcat
      901 taaagggaag agccaggtag aaaggatttt gacggggaac gaaccttgcc ggaatgaacc
      961 cctttcgttc gtccggcgcg ggactgtccg atccgccaaa gtttctggcc cagtttgaat
     1021 cccaggcact ggaacgattc ggaaccggat tattcccgga cggcgtttca ccgatccaaa
     1081 aaacggtcgt cacaatctgc gcatgccacg gaaacttctc cgccggaccc tgcaacggat
     1141 ttgaatcgag atctttgggt tcaactttga aaaggatgct ctcccggagt ttcatcgcgt
     1201 tgtccgtgaa ccgcgcggcg ctatcggtca ctgtctgtgc aaaaagcaca gaagccacgg
     1261 tcgagaataa gccgcacata aaagtcaagg acaacccgtt tccaagcata aaaagccccg
     1321 catcttagaa gcgcctcgca ttcctgtcaa cggcggaaat cgactcaaat ctgacgaaga
     1381 ctacgcgtca acgccaaaac cccgtctaat aacctcggta tttacctctt ggatgcatga
     1441 atttaggcca ataatcacag aaatgagacc acagaggagt caggattcag gagtcaggac
     1501 ttcaggagca ctccacgggc aagaacgcgt gaaaaatctg agaagattcc tgctcagcgg
     1561 atctcgctgt ttcagagaaa tcaggattca ggagcctggg ctattggagt atcgtctcct
     1621 ga
//
LOCUS       DATMKH010000274         2017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_132029,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000274 DATMKH010000000
VERSION     DATMKH010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132029"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..756)
                     /locus_tag="VNV63_03285"
     CDS             complement(154..756)
                     /locus_tag="VNV63_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61674.1"
                     /translation="MVAYGLFTSRREQNRQRYRSDKFYRGTFTIVVIVIVAQVFVVAA
                     ASAILIAEKRIDPATRAIELRMTRLASEEEQYGPLLRKLGILNSLKTLIVNRTPAARL
                     INGIEEAFFANERVGLIELKLSNHFDPNDPNSKDDFNILITGAIKADSLNPTAVLTDF
                     TKTLEGKLPKGANVVIARNASLQGLETFAPFDLRIHYSGQ"
     gene            complement(762..1712)
                     /locus_tag="VNV63_03290"
     CDS             complement(762..1712)
                     /locus_tag="VNV63_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61675.1"
                     /translation="MLQFLSSYPWRYYLKTFFRKGRPARGVLLVKEDRVSMLSPVEGK
                     LVFSTGLRELQGAPSLTNSRRAGLGTEFRRKALADRSVNHLFEWIFVVDTPEMQIHQI
                     DSLRYGSDRELTSRRVMELVPSVLKTDLADWELMNSRLEPIGQDTTPVSILLGLPKSV
                     IRDLEGWSRQFRSTVSAILPLALVLLAQSISLSPTGFHVILTTNHTVIALTREGEIRL
                     ITRVDPINRLGATQIDAIIGSFGDSFPEMAQHRARFISSDLSSAELANEAKDFSIPVV
                     STSESEFSDLEEIIQGYRAAPIEAFALYEALCLVPPNPYH"
     gene            complement(1713..>2017)
                     /locus_tag="VNV63_03295"
     CDS             complement(1713..>2017)
                     /locus_tag="VNV63_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009471981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61676.1"
                     /translation="GMSPAEEKEDIRSTLANSLLLVISQLLLKRVGGGRVAAREIMIQ
                     TASTANLIREGKEKAIEGVLATHREQGMINMTSSIKALYSSRLIDEQTFVENSSTT"
BASE COUNT          464 a          559 c          516 g          478 t
ORIGIN      
        1 cgccctcccg ctgcgtgagg ttgagggtca ttttgctgtt ttccacgatc tggccctgca
       61 attgatcgaa actggcagcc aggaacaaac cctccagagc aacgatggtc agtccgacta
      121 ctaaaaagct caaaaatgca accactgaat gtctcattgg ccggagtagt gaattcttag
      181 atcgaacgga gcgaaagtct ccaacccctg aagcgacgcg tttctagcaa tgacgacgtt
      241 cgcgcccttg ggcaactttc cctccagggt tttcgtaaaa tccgtcagga cagccgtcgg
      301 attcagactg tcggccttga tggcgccggt gatcaggatg ttgaaatcgt ctttactgtt
      361 tgggtcattg ggatcgaaat ggttggagag ctttagctcg attaagccga cccgttcatt
      421 ggcgaagaac gcctcctcaa tgccgttaat gagtcgggcg gcaggagttc ggttcacgat
      481 cagcgtcttg agcgagttga gaattcccag tttccgcaac agcggaccgt attgttcctc
      541 ctcactcgcc aaccgtgtca tacgcagttc aatcgcacgg gtcgccgggt ctattctctt
      601 ttccgcaatc aaaattgcgc tggcggccgc caccacgaaa acctgcgcca cgatcacaat
      661 aacgacaatc gtaaaggtcc cgcggtaaaa cttgtcgctg cggtagcgct ggcggttctg
      721 ctctctccgg ctggtaaata atccgtaagc gaccatcgat attagtggta aggattcgga
      781 ggtaccaggc aaagagcctc gtaaagggca aacgcctcga tcggcgccgc ccggtacccc
      841 tgaatgatct cctcgagatc cgaaaattcc gattcactgg ttgagacgac cgggattgag
      901 aaatccttgg cctcgttcgc gagttccgcg ctggagagat ccgaactgat aaagcgcgcc
      961 cggtgctggg ccatttctgg aaatgaatct ccaaaacttc cgatgatggc gtcgatctgg
     1021 gtagccccaa gcctgttaat tgggtcgacc ctggtaatga gcctgatctc cccttcccgc
     1081 gtcagggcga tgaccgtgtg attagtggtt aagatgacgt gaaatcccgt cgggctcaag
     1141 ctgatcgact gggcaaggag caccagcgca agcggaagaa tggccgaaac agtggatcgg
     1201 aactggcgag accaaccctc caaatccctg atcacgcttt taggcaaacc gaggaggatg
     1261 gaaaccggag tcgtatcctg tccaatcggc tccaacctcg aattcattaa ctcccaatcc
     1321 gccaagtccg ttttcagaac gctgggcacc agctccatga ctctgcggga ggtcaattct
     1381 ctgtcactgc cgtacctgag cgagtcgatc tggtgaattt gcatctcagg tgtgtcaacc
     1441 acgaagatcc attcaaacaa gtgattgacg gatcgatcag ccagggcttt cctccggaac
     1501 tctgttccaa gccctgctct cctggaattg gttaacgaag gtgccccttg gagttctctt
     1561 aaaccggtgg aaaacaccag cttcccttcg accggtgaga gcatcgaaac tcggtcttcc
     1621 ttgactaata aaactcccct cgccggacgg cccttcctga aaaatgtctt gagataatag
     1681 cgccagggat aactggacag aaactgtaac atttaggttg tgctagagtt ttccacaaag
     1741 gtttgctcgt cgattaaccg gctgctgtac agagccttaa tggagcttgt catgttgatc
     1801 attccctgct cccggtgagt cgctaatacc ccttcgattg ctttctcctt gccttcgcgg
     1861 atcaagtttg ccgtcgaagc ggtctggatc atgatctccc gggctgccac cctgcctcct
     1921 cccacccgtt tcaggaggag ttggctaatc accaaaagca gactgttggc caaggtagaa
     1981 cggatatctt ccttttcttc cgccggtgac attccaa
//
LOCUS       DATMKH010000275         3228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2054267, whole genome shotgun sequence.
ACCESSION   DATMKH010000275 DATMKH010000000
VERSION     DATMKH010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2054267"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1025
                     /locus_tag="VNV63_03300"
     CDS             <1..1025
                     /locus_tag="VNV63_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61677.1"
                     /translation="APWYLMQLSINGWEFVDDFILKHNIGRYTGVISGHSGPWFYYLI
                     VLLIGFFPWISFLPAAILSQVPKRVAALRSVQNGEGGPVSGQLGFFLLVWFAVVFLFF
                     SFAQTKLPNYVGPLFPAASILVAQWLIRNFSDQKDLDRSAWLSIGLWIIFTMSLAITL
                     LMVPMLIEKAQQQFSSLPYLTGPVDVGEGPRLLALELLIGSMLGLWLMIRRPFISIVF
                     LAGMMLLFHLTLIHQVLPVVDGYVQRPLKDLALRASRELKGGQLIVYGMNKPSVMFYA
                     NRYAWNVFHPDTVSDHRLQILLDSQERHFVITRVNLLPRFKTMSHFFIVDQQGGYLLA
                     SNQPAM"
     gene            1035..2000
                     /locus_tag="VNV63_03305"
     CDS             1035..2000
                     /locus_tag="VNV63_03305"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012629600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXC61678.1"
                     /translation="METQHKTMNPEISIVVPIFNEEENVEPLVWSVEKVMGSLGRPWE
                     LIFVDDGSTDGTFGKLRAICRSQKSFRVIRLRRDFGQTAALSAGFGHARGGVIITMDG
                     DLQNDPDDIPVLLKLLEEGYDIVNGWRRERKDIWLTKVLPSLIANWLVGRMTGVKLHD
                     YGCSLKAYRSEVIKEIPLYGELHRFIPALAAIHGARITEAPVRHHPRLKGKSKYTPAK
                     AFRVLMDLMIVIFLKTFAQKPFHVFGWMGLIFFLLGVGVDGYLTLEKIFLGMELAGRP
                     MLLFGTLLILAGLQLFSMGILAEIQVRTYHESQGKPIYTIREIIN"
     gene            2140..2904
                     /locus_tag="VNV63_03310"
     CDS             2140..2904
                     /locus_tag="VNV63_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HXC61679.1"
                     /translation="MSNAEESKLALFIDLENIALGARDAKYTTFEINKILERLVEKGK
                     IIVKKAYADWERYKDYKRPFHEAGIELIDIPQKHYSGKNSADIKMVVDAMDLSASKEH
                     VTIFVIASGDSDFTPLVSKLKENDKYVIGVGVKNSTSHLLASNCDEFIYYEDLVRQPK
                     PKPRVVTQLPEKKKDAFQLLMDSIEALQREDKEILWGSMVKQTMKRKLPSFNESYYGY
                     NSFSKLLEDAAKNKLIEITRDPKSRSYIVNPLSEPA"
     gene            2961..>3228
                     /locus_tag="VNV63_03315"
     CDS             2961..>3228
                     /locus_tag="VNV63_03315"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034023 - 5-(carboxyamino)imidazole
                     ribonucleotide mutase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIR carboxylase family protein"
                     /protein_id="HXC61680.1"
                     /translation="MKKPWVGIAMGSDSDLAVMQEAAKLLETFGVSCEVTITSAHRSP
                     QKTLRYIEESEKKGVELFIIGAGGAAHLAGVVAAHSTLPVIGVPM"
BASE COUNT          724 a          805 c          881 g          818 t
ORIGIN      
        1 ccgccccctg gtatctcatg caactctcaa tcaacggctg ggaatttgtg gatgatttca
       61 ttttaaagca caatatcggc cgatacacgg gagtaatctc gggtcacagc ggcccctggt
      121 tttattatct tatcgttttg ctgatcggtt ttttcccctg gatttctttt cttcccgcgg
      181 caattctgtc acaggtccca aagcgcgttg ctgccctaag atcggttcag aatggggagg
      241 gcggaccggt ctccggccaa ctcggttttt ttcttctcgt ctggtttgct gttgtcttct
      301 tattcttcag ttttgcccaa acaaagctcc cgaattatgt tggtcctttg tttccggcag
      361 cctcaattct tgtggcgcag tggttgatcc ggaacttttc tgatcagaaa gatttagacc
      421 ggtctgcctg gctttcgatc ggcctttgga tcatattcac aatgagtctt gccatcacac
      481 ttctaatggt tccgatgctg atcgagaagg cccagcagca gttcagttct ctcccgtacc
      541 tgaccggccc ggttgatgtg ggtgaaggcc cccgactcct ggcgcttgag ctcctgatcg
      601 gatcgatgct cggcctttgg ctgatgatcc ggcggccttt catcagcatt gtcttcctgg
      661 ccgggatgat gctgctcttt cacctcacgc tgatccatca ggtgcttccg gtagtcgatg
      721 gatatgttca gcgacctttg aaagatttgg cgctgcgggc gagccgtgaa ctcaaaggcg
      781 gacaactgat cgtgtacggg atgaacaaac cttctgtcat gttttatgca aatcgttatg
      841 cctggaacgt gtttcatccg gataccgtct cggaccatcg gcttcagatc ttgctcgact
      901 ctcaagagcg tcattttgtg attaccagag tcaacctctt gccgaggttt aagacgatgt
      961 cgcatttttt cattgtggat caacagggag gctacctttt ggcgtcgaat cagccagcca
     1021 tgtgaggatg cgagatggag acccagcaca aaacgatgaa tcctgagatc tcgatcgtcg
     1081 tcccgatttt taatgaggaa gagaatgttg aacctctagt ctggtccgtt gaaaaagtga
     1141 tgggctctct cggacggccc tgggagctga tctttgtgga tgacgggagc acggatggga
     1201 cgtttggaaa gcttcgggcg atttgccgga gccagaaatc gttccgagtg attcgcctcc
     1261 gccgggattt cggtcagacc gccgcgctgt ccgccgggtt tggccatgcc cggggagggg
     1321 tgattatcac gatggatgga gatctccaga acgatccgga tgacattccg gttctcttga
     1381 aattactgga agaaggatac gacatcgtca acggctggcg aagagaacgg aaggacatct
     1441 ggctgaccaa ggttctgcca tctctgattg cgaactggct cgtcgggagg atgaccggtg
     1501 tcaagcttca cgactacggc tgctcactca aggcctaccg ttccgaggtg atcaaggaga
     1561 tcccgttgta cggtgagctt catcgtttca tcccggccct cgctgcgatc cacggtgccc
     1621 gtattaccga ggctccggtg cggcatcatc cgaggctgaa agggaagagc aagtacacgc
     1681 cggccaaggc cttccgtgtt ctgatggatc ttatgattgt gatcttccta aagacatttg
     1741 cccaaaaacc cttccatgtt ttcggctgga tgggtctgat ttttttcctg ctcggtgtgg
     1801 gcgtggacgg ctatctgacg cttgaaaaaa tattcctggg aatggaattg gccgggaggc
     1861 cgatgctcct cttcgggaca ctgctcattc tggccgggct tcagctcttc tccatgggaa
     1921 ttttggctga gattcaggtg aggacttatc atgaatcgca ggggaagccg atatacacta
     1981 ttcgggagat tataaactga gaccggcatg gcatttggtt taagctggtt tttaattctg
     2041 ttcgcctcgg ggatgatcgg cggcatcgcc tggatcatgt ctccggagca tttgactgga
     2101 atgaaataac cgtctagaat gacatcagga gataatcgca tgagcaacgc agaagaatcc
     2161 aaactcgcgc ttttcataga tcttgagaac atcgcgctgg gcgcgcgcga tgccaaatac
     2221 acgactttcg agatcaacaa gatcttggag cggctggtgg aaaaggggaa gatcatcgtc
     2281 aagaaggctt atgcggactg ggagcggtat aaggactaca aacggccgtt tcacgaggcc
     2341 ggcatcgagc tgatcgacat accccaaaaa cattacagcg ggaagaacag cgccgatatt
     2401 aagatggtcg tggacgcgat ggacctttcg gcttccaaag aacatgttac tatcttcgtg
     2461 atcgcctcgg gggacagtga cttcaccccg ctggtctcaa agcttaagga aaacgacaaa
     2521 tacgtgatcg gtgtgggcgt caagaactcg acctcccatc tgcttgcaag caattgcgat
     2581 gagttcatct actatgaaga tcttgttcga cagccgaaac cgaaaccgag ggtcgtgact
     2641 caacttccgg aaaagaaaaa ggacgccttc cagcttctca tggactcgat cgaggcgctt
     2701 cagcgggaag ataaagaaat cctctggggt tcgatggtga agcagacgat gaaacgcaaa
     2761 ctgccctcgt tcaacgagag ctactacggc tataacagct tcagcaagct cctggaagat
     2821 gccgccaaaa ataaactcat cgagattacg cgcgacccca agagccggtc gtacatcgtc
     2881 aatccgcttt cggagccggc ataaactttt gctgtcccta gtcttgacag ttgaacgcct
     2941 gtgtgatacc gtctgacttc atgaaaaagc cgtgggtagg tattgcaatg ggaagcgatt
     3001 cggacctggc cgtgatgcag gaggccgcca agcttttaga aacttttggg gtctcctgcg
     3061 aagtcacgat cacctcggct catcgttctc ctcagaagac tttgcgctac atcgaagaat
     3121 ctgagaaaaa aggagtcgag ctatttatta tcggggcggg cggagcggcg catctcgccg
     3181 gtgtagtcgc ggctcattca acgcttccgg tgatcggcgt tccgatgg
//
LOCUS       DATMKH010000276         1119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_132732,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000276 DATMKH010000000
VERSION     DATMKH010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132732"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..462
                     /locus_tag="VNV63_03320"
     CDS             91..462
                     /locus_tag="VNV63_03320"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXC61681.1"
                     /translation="MPKSILIADDNAGVRSLIRTYLEMQTEFDICGEAADGVDAVEKI
                     KELNPDLVLLDLAMPRMNGAEVASIIKVSMPHLPIILFSVHGEKIGKALASAVGVDVV
                     LSKPDGIANLVESVRSLLGPP"
     gene            complement(760..>1119)
                     /locus_tag="VNV63_03325"
     CDS             complement(760..>1119)
                     /locus_tag="VNV63_03325"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HXC61682.1"
                     /translation="QKLFGWFSAPGFGTPGINGSLAQLTARNIPKFMAWLIIIGQSFG
                     SIALMSGFLGRIAAGGLFIIFTGALIVHLPDGWTLNWFGSKKGEGIEYFVLLLSVLLV
                     IVLKGSGALSIDCWLTR"
BASE COUNT          286 a          275 c          301 g          257 t
ORIGIN      
        1 ccaaaactac agtaagaccc gcattacacg cccgctgtga agagtggaag atgtaggaag
       61 gcttcctcaa actgagaaag gaacggtgac atgccgaaaa gtatcctcat cgcggatgat
      121 aatgctggcg tgcgatctct aattcgcacg tatttggaga tgcagacaga atttgatatt
      181 tgcggagaag ctgcggacgg cgtggatgcc gtcgagaaaa taaaggaact caaccctgat
      241 ctggttctct tggatttggc gatgcctcgg atgaacggtg cggaagtggc gtcgatcata
      301 aaagtctcga tgccgcatct tccgatcatt ttgttttccg tgcacggcga aaaaatcggg
      361 aaggcgcttg cttcggccgt gggcgtcgat gtcgtgctgt ccaaaccgga tgggatcgca
      421 aatcttgtag aaagcgttag aagtcttctg ggaccccctt gagccttgat ttgtttctgc
      481 cgaagtacga agctgggatg cgacatggtg tgatgcgggg cttctgttaa taaggaatgc
      541 ggatgacttc agggcggccc gtaaattctt ccagacttgg cggcaggcgc ggagctcgct
      601 tcttacgatc cgtctcctaa tatgcccgac aaagcgatca ttttcgagtc agtgtcgatc
      661 cacgggcaac ggcagggaat gaagaactcc ccagggtcat caagagcgga gatttgccaa
      721 agtggctgtg gattctgatg tgcttgccgc ccacgcatat catcttgtca gccaacaatc
      781 tattgatagc gcaccactgc ccttgagaac gatgaccaag agaactgaca ggagcaatac
      841 aaaatactca attccttctc ccttttttga tccaaaccaa ttcagtgtcc acccgtccgg
      901 cagatgcact atgagtgcac cggtgaagat gatgaacagt ccaccggccg cgattcttcc
      961 caaaaagccg gacataaggg caatacttcc gaacgactgc ccgatgataa tgagccaggc
     1021 cataaattta ggaatgttcc tcgccgtgag ctgtgcaaga gagccgttga ttcctggggt
     1081 gccaaatccc ggggcactaa accatccgaa aagcttctg
//
LOCUS       DATMKH010000277         4646 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2055152, whole genome shotgun sequence.
ACCESSION   DATMKH010000277 DATMKH010000000
VERSION     DATMKH010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4646
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2055152"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1520)
                     /locus_tag="VNV63_03330"
     CDS             complement(<1..1520)
                     /locus_tag="VNV63_03330"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009381802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HXC61683.1"
                     /translation="MTKYIFITGGVVSSLGKGLAAASIGNLLESRGLSVTFLKLDPYI
                     NVDPGTMNPYQHGEVFVTADGAETDLDMGHYERFTTVTMAQKNNYTTGRIYHEVISKE
                     RRGDYLGGTVQVVPHITDEIKRCIRAVANQKDIVIVEVGGTVGDIESLPFLEAVRQFP
                     YDIGKDNVLYIHLTLVPFIKAAEELKTKPTQHSVNKLREIGIQPHILLCRTDRMLPIE
                     LKKKIALFCNVDKEAVITAKDVESIYEVPLLFHQEGLDEIIVRQLRLKSQKPDLDDWE
                     QMVHRIKNPRHHVRIALVGKYIELKESYKSLSEALSHGGISHETRVEVDWVDAEHLER
                     DGTERHLKGIQGVIVPGGFGGRGIEGKIMAIQHAREQKVPFLGLCLGMQCAVIEFARH
                     EAGFKTADSSEFDAKTTDPVIDLLSTQRGVKSKGGTMRLGSYPCHLEKGTLAYSIYGH
                     AEVSERHRHRFEFNNRYRKSLTKAGLVISGIYKKENLVEIVELRDHPWFLATQFHP"
     gene            complement(1517..2266)
                     /gene="kdsB"
                     /locus_tag="VNV63_03335"
     CDS             complement(1517..2266)
                     /gene="kdsB"
                     /locus_tag="VNV63_03335"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HXC61684.1"
                     /translation="MRVVLVIPARFSSTRFPGKPLADLLGRPVIQHVYEQASSLKVVD
                     RVIVATDDERIMKVVERFSGEAHLTSNRARTGSDRVAELARRMKAELFVNLQGDELLL
                     DTEMLVPLIDLFLKKKSLLVGSLKRSLESEKDIKNPNIVKVVSDRKDFALYFSRAPIP
                     FFRDRSIPLPLKNIYQHLGVYIYRRQPLLEFSDWPTGTLEDFEQLEQLRFLENGYRIK
                     LLETQGRSLRIDTPEDLKTAEAVLSQKERSQ"
     gene            complement(2269..3273)
                     /gene="rfaE1"
                     /locus_tag="VNV63_03340"
     CDS             complement(2269..3273)
                     /gene="rfaE1"
                     /locus_tag="VNV63_03340"
                     /inference="COORDINATES: protein motif:HMM:TIGR02198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HXC61685.1"
                     /translation="MKKENDRIEASSLKRYLSRFHRARLLVLGDLMLDHYIWGKVNRI
                     SPEAPVPVVNVTSESLLLGGAANVANNVLSLGGRVELCGVIGPDDAGRKFLHVLGQAG
                     LSVDGIVVEKERQTTKKTRIIAHSQQVVRFDREEREKISKDSQQRLCDFIQDRSKSVD
                     GIIISDYAKGVITPNLMQWLMTLTRRRGISVIVDPKVSHFDLYQGTTVVTPNHLEAAQ
                     ASGIETDGGNGLITAGKMLLKRLGSEAVLITRGEHGMSLFERRGTVTHIPTMAKAVFD
                     VTGAGDTVISTLGLALAAGAQMKEAAFLSNLAAGIVVGIVGTATVNREALETALKRGW
                     "
     gene            complement(3273..3932)
                     /gene="lepB"
                     /locus_tag="VNV63_03345"
     CDS             complement(3273..3932)
                     /gene="lepB"
                     /locus_tag="VNV63_03345"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015725809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HXC61686.1"
                     /translation="MIDRAQERKNIKSVIREYGEAILIAVVLALLIRTFIVQAFKIPS
                     GSMIPTLLIGDHILVNKFIYGSHIPFTDATVFAMTMPKRGDVIVFKYPDDETKDFIKR
                     VIGEPGDTLEVKDKAVYVNGKALNDEHYAIHQDPTVFPRGNQPRDNMGPIVVPEGDYF
                     VMGDNRDQSLDSRFWGFVKADKIKGRAFRIYWSCDAQESWVCLFDLRKIRWNRIGKAI
                     Q"
     gene            complement(3932..>4646)
                     /locus_tag="VNV63_03350"
     CDS             complement(3932..>4646)
                     /locus_tag="VNV63_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844537.1"
                     /note="back-translocating Elongation Factor EF4; binds to
                     the ribosome on the universally-conserved alpha-sarcin
                     loop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HXC61687.1"
                     /translation="LQLISTAPTVVYRVTLKNGEIVLIDNPAKLPEPQKVLKFEEPYI
                     LTMIITPEAHLGPLLGLCQDRRGVQRELKYLDRDRVLLSYEIPLNEVVMDFYDQLKSK
                     SQGYASLDYEFLGYRESDLVKVDILLNGETVDALSFITPREKAYTRARQLAEKMRTLI
                     PQQLFEVAIQATIGQKIIARETVAALRKNVTAKCYGGDITRKRKLWEKQKEGKKRMKQ
                     VGRVEIPQEAFLALLKVQD"
BASE COUNT         1069 a         1290 c         1065 g         1222 t
ORIGIN      
        1 tccggatgaa attgagttgc caagaaccaa ggatgatctc ggagttcgac gatttcaacc
       61 aggttctcct ttttatagat accgctgatc accagtcccg cttttgtcag agactttcgg
      121 taacgattat taaattcaaa ccgatggcga tgacgctcag aaacttctgc atgtccatag
      181 attgaatacg ccaaggttcc tttttccaaa tgacaaggat aactgcccag tctcatggta
      241 ccacctttac tttttacacc ccgctgagtc gagaggagat cgatgaccgg gtccgtggtc
      301 ttggcatcaa attcggagct gtccgccgtt ttgaatcctg cctcgtgacg tgcaaactca
      361 atcacggcac actgcatacc gagacacaaa cctaagaaag gaaccttctg ttccctggca
      421 tgctgaatcg ccatgatctt gccttcgatc cctcttcccc cgaatcctcc cgggacaatc
      481 acgccctgaa ttcccttgag atgccgttcg gtgccatcgc gttccagatg ttcggcatcc
      541 acccagtcca cctccacacg cgtctcatgg gaaatgccgc cgtggctcaa ggcttccgag
      601 aggcttttgt aagattcctt gagttcgata tatttaccca ccaaagcgat gcgcacatga
      661 tgacgcgggt tcttgatccg atgcaccatc tgctcccaat catcgagatc gggcttttgg
      721 gattttaagc gaagctggcg aacgatgatt tcatcgaggc cttcttgatg aaagagtaac
      781 gggacctcat agatgctctc cacatctttg gcagtgatca ccgcttcttt atccacatta
      841 caaaacagcg cgatcttctt tttgagctcg atcggaagca tacggtccgt ccggcaaagc
      901 agtatgtggg gctggatacc aatttccctc agcttgttca cactatgctg agtaggtttg
      961 gtcttaagct cttcggccgc tttaatgaag gggaccagcg tcaagtgaat atacaggaca
     1021 ttatctttgc caatgtcata aggaaactgg cggaccgctt ctaggaacgg gagactttcg
     1081 atgtctccaa cggtgcctcc cacttctaca atgacaatat ccttttgatt cgcgactgcg
     1141 cggatgcagc gtttgatctc gtctgtgatg tgcggtacaa cctgcaccgt gcctcccagg
     1201 tagtctcccc gacgttcttt ggaaatgacc tcgtgataga ttctccccgt ggtgtagttg
     1261 tttttctgcg ccatcgtaac ggtcgtaaat cgttcatagt ggcccatgtc cagatcggtt
     1321 tccgcaccgt ccgccgtcac gaagacttcc ccgtgctggt aagggttcat cgttccagga
     1381 tccacattga tgtacggatc aagcttaagg aaggtcacgg aaaggccccg gctctctagg
     1441 agatttccaa tcgaagcagc ggccaaccct ttccccaagg aagagacgac accgcctgtg
     1501 ataaagatat acttggtcat tgggatcgtt ctttttgact caagacagcc tctgcggttt
     1561 taaggtcctc cggagtatca atacggagcg aacgaccctg agtttcgaga agcttgattc
     1621 gatagccatt ctccaggaat ctcagctgct ccaattgttc aaaatcctcg agggtaccgg
     1681 ttggccagtc ggaaaactca agaagaggct gtcgacgata gatatagacc cccaggtgct
     1741 ggtagatgtt tttcagcggg agtggaatcg aacgatctcg aaagaacgga atcggtgccc
     1801 tggagaaata tagtgcaaaa tcttttcgat ccgataccac cttgacaata tttgggtttt
     1861 taatatcttt ttcagactca aggctcctct tcaagcttcc gaccaggaga gatttctttt
     1921 ttaggaagag gtcgattagc gggaccaaca tttctgtatc caggagtagt tcatccccct
     1981 gcaggttgac gaataattct gccttcatcc gccgggccaa ctccgcaacc cggtcactcc
     2041 cggttcgggc gcgattcgat gtgagatggg cttctcctga aaacctttcg acaaccttca
     2101 taatccgttc atcatctgtc gcaacgatca cgcgatctac aactttcaag ctgctcgctt
     2161 gttcgtagac atgttgtatc acgggacggc ccaagaggtc cgctaaaggt tttccaggaa
     2221 accgcgttga tgaaaatcgt gccggaatga ccagtaccac tcgcattgtt accagcctcg
     2281 tttcagtgcg gtttccaagg cctcccggtt gaccgtggcg gtgcccacga ttcccaccac
     2341 aattcctgcc gctagattgg acagaaacgc cgcctccttc atctgtgccc ctgccgccaa
     2401 ggctaatccc agtgtgctga tcactgtgtc tcctgcgcct gtcacatcaa agaccgcctt
     2461 ggccattgtc ggaatatggg tcaccgttcc acgacgttcg aacaggctca tgccgtgctc
     2521 accccttgtg attaagaccg cttcgctgcc taaacgcttc agaagcatct tccccgcggt
     2581 gatcagaccg tttcctccat ccgtttcgat tccggaggcc tgtgccgctt ccaggtggtt
     2641 gggggtcacc acggtagtcc cctgatagag atcgaagtga ctgacctttg ggtccacaat
     2701 cacgctgatg cctctgcgcc gcgttaaagt catcagccac tgcatcagat tgggcgtgat
     2761 cactcccttg gcataatccg agatgataat cccatccaca ctcttgctcc gatcctgtat
     2821 gaaatcacag agcctctgct gactgtcttt ggaaattttc tcgcgctctt cccgatcaaa
     2881 ccggactacc tgctggctgt gggcgataat ccgcgttttt tttgttgttt gtctctcctt
     2941 ctccaccacg atgccgtcaa cactcagacc ggcctgaccc aggacatgca aaaattttct
     3001 cccggcgtca tccggaccga tcacgccgca cagttctacc cttcccccta acgagaggac
     3061 attatttgct acgttagcag cgcctcccag caacagcgac tcggaggtca cattcaccac
     3121 cggcaccggg gcctccgggg aaatccgatt aacttttccc caaatatagt gatccagcat
     3181 gagatcgccc aggacgagca gtcgggcccg gtgaaaacgt gagagataac gttttaaaga
     3241 acttgcctca atccgatcat tttctttttt cattactgga tggctttgcc gatacggttc
     3301 cagcggatct tcctcaaatc aaacaaacag acccaactct cctgcgcatc acaggaccag
     3361 tagattcgaa aagcccgtcc tttgatttta tccgctttga caaatcccca aaaccggcta
     3421 tcgaggctct gatcccgatt gtcgcccatc acaaaatagt ctccttcggg gaccacgatt
     3481 ggtcccatgt tatcgcgcgg ctggtttcct cgtgggaaaa ccgtcgggtc ctgatgaatc
     3541 gcatagtgtt catcattcag ggccttgccg ttcacgtaga ctgctttgtc tttaacctct
     3601 agggtatctc ccggttcgcc gatcacccgc ttgataaaat cttttgtttc atcatcggga
     3661 tatttaaaga cgatgacgtc tccccgtttg ggcatcgtca tcgcaaagac ggttgcgtcc
     3721 gtgaacggga tatgagatcc ataaataaat ttgttcacca ggatatgatc accgatcaac
     3781 agggtcggaa tcatggaccc cgatgggatc ttaaaagcct gaacgataaa agtccggatc
     3841 aaaagggcca agaccactgc gatgagaatg gcctcaccgt attcccggat aaccgatttg
     3901 atattttttc tttcttgggc ccggtcgatc actagtcctg aaccttcaag agggcgagaa
     3961 aggcctcctg gggaatttct acccgcccca cctgtttcat tctttttttc ccttcctttt
     4021 gtttctccca taatttcctc tttcgtgtga tatcccctcc gtaacatttc gccgtcacat
     4081 tttttcgaag cgctgcgacg gtttcccgtg caatgatctt ttggccgatt gtggcctgaa
     4141 tcgccacctc aaaaagctgt tgtggaatga gtgtccgcat cttttcggcc aattgtcgag
     4201 cacgtgtata agccttctcc cgaggggtga taaaggacaa ggcatctacc gtttcaccat
     4261 tgagtaagat atctactttc accagatcgg attctcgata tccaaggaac tcataatcca
     4321 gagacgcata tccctggctc ttcgacttta gttgatcata gaaatccatg acgacctcat
     4381 tgagcggaat ttcgtaactc agaaggaccc gatcacgatc cagatacttg agttcgcgtt
     4441 gaaccccccg ccggtcttga cacaacccaa gaagcgggcc gagatgggcc tccggggtaa
     4501 tgatcatcgt caggatatag ggttcctcaa actttaaaac cttttgcggt tcaggaagct
     4561 tcgccgggtt atcaatcaaa acgatttccc cgttttttaa agtcacccga tagacaaccg
     4621 tgggagcggt gctgatcagc tgtagt
//
LOCUS       DATMKH010000278         1595 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_454916,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000278 DATMKH010000000
VERSION     DATMKH010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454916"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="VNV63_03355"
     CDS             <1..618
                     /locus_tag="VNV63_03355"
                     /inference="COORDINATES: protein motif:HMM:NF019869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HXC61688.1"
                     /translation="WRIVNGHAERVKASKKLKGHDEAESDYAKTLRQATHRTIQKVTN
                     DLEDHFQFNTAIAALMEFYNTTSDTITKWEKSPSKEMEQPALIRAITHALETLVHLLS
                     PFAPHIAEELWEKLGHPPQAETSLVKQPWPSYDEAMLKHEEINIAVQVNGKLRGVLTV
                     QAELSEEKIKERAFADAKIAAWLQGKKVAKVIYVQDRLLNIVVKD"
     gene            647..1219
                     /locus_tag="VNV63_03360"
     CDS             647..1219
                     /locus_tag="VNV63_03360"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HXC61689.1"
                     /translation="MRFIKNLTRSTRFLLLLGGLLLILGCGYQFVGRASTLPPEIRKI
                     AVPIIENNTFEPLLDERLTEAIKEGFIARPRLQVVNSASEADAVFKGKITAFRLIPLS
                     FNPQNEAAEYRVKILGDFTLQDIHDQKILWQENMLEITADYFVIQDLLDPTRVDIART
                     RDAEDRAIAEAAKILAENLISRILEGFPSH"
     gene            1312..>1595
                     /locus_tag="VNV63_03365"
     CDS             1312..>1595
                     /locus_tag="VNV63_03365"
                     /inference="COORDINATES: protein motif:HMM:NF017911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HXC61690.1"
                     /translation="METIKSSAIQPEAKDFNLETLYGDETSAKNIIETAETLPAFSER
                     RLLIIKRLDALPTRQQEALLPYLAKPCPTTCLVLVAEKIDQRKKFFQTLKT"
BASE COUNT          453 a          426 c          381 g          335 t
ORIGIN      
        1 tggcggatcg tgaatggaca tgccgagcgt gtcaaagcct ctaaaaaatt aaaaggccat
       61 gacgaggcag aaagtgatta tgcgaagact cttaggcagg caacacatcg aacgattcaa
      121 aaggtcacaa acgacctgga agaccatttc cagttcaata ccgcaatcgc ggccttgatg
      181 gaattctata acacgacctc agacaccatc acgaaatggg aaaaatctcc ttccaaagag
      241 atggagcagc cagccctcat acgggccata actcacgcac tggaaacact cgtccatctc
      301 ctttctccct tcgctcccca cattgcggaa gaactttggg aaaagttagg acatccgcct
      361 caagcggaga ccagcctcgt gaaacaaccg tggccgagtt atgatgaagc gatgctcaag
      421 cacgaagaga tcaatatcgc ggtccaggtc aacggcaaac tccgcggcgt cttgaccgta
      481 caggcagaat taagcgaaga aaaaattaaa gagcgcgcct tcgcggatgc aaagatcgcc
      541 gcttggcttc agggaaaaaa ggtcgcgaag gtcatctacg ttcaagaccg gcttcttaac
      601 atcgtggtaa aagactaaat gagcgggtgg ccgcgaaagt taacgaatgc gattcattaa
      661 aaatttgact cgatccacac ggtttctctt actcctcggc ggtctacttc tgattctagg
      721 ctgcggctat caatttgttg gacgagcctc cacccttcca ccggaaatta gaaagatcgc
      781 agtccccatc atcgagaaca acacgttcga gccgctctta gatgagaggc tcaccgaggc
      841 aatcaaagaa ggcttcatcg cccggccccg attgcaggtg gtcaacagcg cctccgaggc
      901 ggatgctgta ttcaagggca aaatcaccgc ctttcgtctc ataccgctct ctttcaatcc
      961 ccagaacgag gcggccgaat atcgggtgaa aatcctgggc gattttacgc tccaagatat
     1021 ccacgatcaa aagattcttt ggcaggaaaa tatgttggaa ataaccgccg actattttgt
     1081 gatacaagat ctactggacc cgacacgcgt agacatcgcc aggactcgtg atgccgaaga
     1141 tcgagcaatc gcagaggccg caaagatctt ggccgagaat ctgatcagcc ggatactgga
     1201 gggattccca tcgcactgag acctcaggac cccattttaa aactcaaacg cggggaggtt
     1261 ggacctctct atctgctcta tggagaagag gaattcctga tccaggaggc gttggaaacc
     1321 atcaagagtt ccgccatcca gccggaggcc aaagatttta atcttgagac cctctatgga
     1381 gatgagactt ctgcaaagaa tattatcgag accgccgaga cgctgccggc cttctctgaa
     1441 cgccgcctct tgatcatcaa gcgtctggat gctcttccaa ccagacagca ggaagcactt
     1501 ttgccatatc ttgccaaacc ctgtcccaca acctgcctgg tgctcgtggc ggaaaaaatt
     1561 gaccagcgaa agaagttttt tcagactctt aaaac
//
LOCUS       DATMKH010000279         1888 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2698408, whole genome shotgun sequence.
ACCESSION   DATMKH010000279 DATMKH010000000
VERSION     DATMKH010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2698408"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            202..987
                     /locus_tag="VNV63_03370"
     CDS             202..987
                     /locus_tag="VNV63_03370"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HXC61691.1"
                     /translation="MSWYYGVDGRHQGPVEERALETLELSGAIEWTTPIWHEGMAAWK
                     PFGEIFQRPSVQCHECNRTVDKESAIHYRDLSICPRCKISFFQKVCEGLASEEAVHYC
                     GFWIRFCAKMLDGLILMAATLPLSVINQVIFFRFYPITGQGDRIESLENMTNLGTFLT
                     IQAAFVLVSLILSFAYEVYFVGRLGGTPGKLLLRMRIVRSDFSRLTYRRAAIRFFAKM
                     LSDLTMYIGYLMVGIDPQRRALHDYIADTRVIERDRPVTPASR"
     gene            complement(1152..1373)
                     /locus_tag="VNV63_03375"
     CDS             complement(1152..1373)
                     /locus_tag="VNV63_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61692.1"
                     /translation="MKVTNRLTTLRETDRRPGTGILVEFRENVGLDLGMTGRALTGQP
                     GREQIYWEGFAAARTGEIGGHLAYDTGRG"
     gene            1482..1763
                     /locus_tag="VNV63_03380"
     CDS             1482..1763
                     /locus_tag="VNV63_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61693.1"
                     /translation="MRKQRDEDGLVQYAALFDNVALFLVTAPVIIFFFWFLTILSAPV
                     SLFLSFYYWPRQWNLLPRSRFRYGLAILFSLLLIGLWAFVLYYIATHRK"
BASE COUNT          408 a          535 c          462 g          483 t
ORIGIN      
        1 tcgcccctgg ctgcattttc tcggccaacg gaaaaaattt tcgcaactgc atgcgcatca
       61 gctgcggaca cccgtggtcg gctcgcatcg aacgagcaat caaaaccctt gggtacttgg
      121 caagtaagta gcgtcccgct ttgcttgcac cctccattga cgtcgcccca gctttccgcg
      181 agcgtttcca ctcaagaatc tatgagttgg tactatgggg tagacggacg acaccaaggt
      241 cccgtcgagg aacgagcgtt ggaaactctc gagctctccg gggctatcga atggaccact
      301 cccatctggc acgaggggat ggccgcctgg aaaccgtttg gagaaatatt ccagcgccca
      361 tcggttcaat gccacgaatg caaccgaacg gtcgataagg aatcagccat ccattatcgg
      421 gatctctcta tctgccccag atgcaagatc tcgttcttcc aaaaggtttg cgaaggattg
      481 gccagcgaag aggcggtcca ctactgcggg ttctggatcc ggttttgcgc caaaatgtta
      541 gatggattga tcctcatggc cgcgaccctt cctttgtccg taatcaatca ggtgatcttt
      601 ttccggttct atcccatcac gggtcaaggg gacagaattg agtcgttgga aaacatgacc
      661 aacttaggca cctttctaac gattcaggca gccttcgtcc tcgtcagtct aatcctgagt
      721 ttcgcgtacg aagtttattt cgtcggacga ttgggcggaa ctccggggaa acttctgctc
      781 cggatgcgga ttgtgcgttc tgatttttcc cgactcactt accgcagggc agcgattcgt
      841 ttttttgcaa agatgctcag cgacctcacc atgtacatcg gttacctcat ggttggaatc
      901 gatccgcagc gtcgagcgct tcacgactac atcgctgata ctcgcgtgat cgaacgcgac
      961 cgacccgtga ccccggcctc aagatgatat ccgtcaaacc cggaaatcgc gggagaagga
     1021 taaaacacag atttcgcaaa ttaaagagac ggagtctttg gcttggcgcg tggcgtaaac
     1081 tcaccttgga caaaaataaa atgcttaaga cacaccccat accccctcct caaatctgtg
     1141 taatctgtcg tttaacctct tccggtgtcg taggctagat gcccgccaat ctcacctgtc
     1201 ctcgctgcag cgaatccctc ccagtagatc tgttcgcgtc cgggctgccc ggtcagtgcc
     1261 cggcctgtca ttcccaggtc gaggcctaca ttttcccgga attcgacaag gatcccggtt
     1321 cccggcctgc gatccgtctc gcggagagtc gtcaggcggt ttgttacttt cattctcaat
     1381 accgtgcgat gagtccgtgc gacaactgcg gccgattcct ctgtgagatc tgctcgatca
     1441 gtgtaggcag cagacaactc tgcgcggagt gcctttcaaa attgagaaaa caaagggatg
     1501 aagacgggct cgttcaatat gccgctttgt tcgacaacgt cgctctgttc ctggtgaccg
     1561 ctccggtgat catctttttt ttctggtttc tcacgatttt atccgcgccg gtctcattgt
     1621 ttttatcgtt ttactactgg ccaagacaat ggaacctact gccccggtca cgctttcgtt
     1681 acggactagc gattctgttc tccttgctcc tgattgggtt gtgggcgttt gtcctctact
     1741 acatcgcaac ccatcggaag taggccactc ccaatccgaa accctatgtc ctataccaag
     1801 atctccggcg gcggactgtt aatgaccttt attccaggct tgaggaccaa gctctatcta
     1861 gggacagatc accttctctt gattgaac
//
LOCUS       DATMKH010000280         3739 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1414014, whole genome shotgun sequence.
ACCESSION   DATMKH010000280 DATMKH010000000
VERSION     DATMKH010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3739
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1414014"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..159
                     /locus_tag="VNV63_03385"
     CDS             <1..159
                     /locus_tag="VNV63_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HXC61694.1"
                     /translation="GGLTTFSTFSAESVTLFARSQYGWGFGIIALHVIGSLTMTALGI
                     FTISLIKP"
     gene            177..512
                     /locus_tag="VNV63_03390"
     CDS             177..512
                     /locus_tag="VNV63_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HXC61695.1"
                     /translation="MGKYLKFYVHENRRHHSILLYEWLLEQAKHLGIPGGSAFRSIAG
                     FGRLGVINEEHFFELAGDLAVEVVFAVSNEEADRLLDLVRAENLALFYTKMSIEYGVA
                     NGNAEPPDG"
     gene            622..2361
                     /locus_tag="VNV63_03395"
     CDS             622..2361
                     /locus_tag="VNV63_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXC61696.1"
                     /translation="MQSNVDLKRLWVYVRPYLKPLTLSAVLLSATAVINLALLWVLRN
                     AFNDILVEHSQTALSAKVLILFSLFLIQSILSMGHNYLITWIGQRVVTDFRCKLFEHL
                     QRLSLSFFAKRRTGELISRFTNDVGIIQNMIVNVPVDTVKQTVTLLGGIAILFYVNWK
                     LCLMVLTLLPLIALTARYFGKKLRRLSTQIQDQLADSSTVLEEVISGIRMVKSFVQEE
                     YELRRFSRQIERVLDITMQKTKVLAVFVPVITFLTMGAGAGVIWYGGNQVIQGHITPG
                     DLFAFALYAGLMIGPFGAFARLFSQIKEAQGATQRVFEILDTQPLILDLPDAKPMPTI
                     RGQVAFHQVSFAYDPDIPVLKDLSFEVEPGQVVALVGPSGSGKTTLVNLLHRFYDPTA
                     GWIEIDGQDIKKIQLRNLYTQIALVPQETILFGGTIRENIMYGRTEASEEEMMAAAKA
                     ANAHDFIMAFPKGYDTIVGEKGINLSGGQRQRLAIARAILKNPRLLILDEATSSLDNE
                     SEKLVQEALDRLMENRTSFIIAHRLTTIQHADRIFVLDKGKLVEEGTHAELLDRKGLY
                     HHLYTMKLAGVEV"
     gene            complement(2362..2841)
                     /locus_tag="VNV63_03400"
     CDS             complement(2362..2841)
                     /locus_tag="VNV63_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61697.1"
                     /translation="MKKNMIMFLIVFGAMAWAVPSSYAGVGVNINIGVPVPPPVVVEA
                     PPEMVIMPETGVYVAVGTPYDLFFLGGRYYYFHDGYWYGSRGYGGPWVHVERRGLPVG
                     LRKYKIERLHAFRDHAWKEYHERGPEFRGKHFKAEARERREERKEIRGERGEERRER"
     gene            complement(2931..3362)
                     /locus_tag="VNV63_03405"
     CDS             complement(2931..3362)
                     /locus_tag="VNV63_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61698.1"
                     /translation="MIKKILLVLLVGIIFHAGYQVLKYYKVSEGFSDDLDTLMLTVRG
                     QTEESFKQEVIARAEKNGIHLTPEEVSVRIEDTAASSFGSRVLGGTGAAVHNKRITLD
                     FEYHIPIYGIPLTHPVHRTKIFAAEIGSPSSLQEEQIKQVE"
     gene            complement(3366..>3739)
                     /locus_tag="VNV63_03410"
     CDS             complement(3366..>3739)
                     /locus_tag="VNV63_03410"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HXC61699.1"
                     /translation="LFDSGSAEIKPEGLKVFDRVGEILKKVKDKQIRVEGYTDDVPIG
                     PKLMSKFPTNWELSMTRATNVVRYLEDKVKLDPKMLSAAGYAMNHPVASNDTKEGKAQ
                     NRRIEIVLLPQDIDQVLQELK"
BASE COUNT          867 a         1009 c          938 g          925 t
ORIGIN      
        1 ggagggctca ccactttttc cacgttttct gcggaatccg ttacactttt tgcaagaagc
       61 cagtacggct ggggattcgg catcattgcg ctgcacgtga tcggttccct cacgatgacc
      121 gcgctcggca tctttaccat aagcttgatc aaaccataag gaggcaaact tgcatgatgg
      181 gaaaatatct aaaattctat gtccacgaga accggagaca tcactccatc ctgctctacg
      241 aatggctgct ggagcaggct aagcacctcg gcatccctgg cggttcagct ttccgttcca
      301 tcgccggctt tggacgtctc ggtgtaatca acgaagaaca tttcttcgag cttgcggggg
      361 atttggcggt cgaggtcgtg tttgcggtca gcaatgagga agcggatcgc ctgttggact
      421 tggtccgggc tgaaaacctg gccctctttt acacgaaaat gtcgatcgaa tacggagtcg
      481 cgaacgggaa cgctgaaccg cctgatgggt aaacgcagtt cctagatatg ccatgatcaa
      541 accccaagcg cgctgggacg atggtgaagt aaccgtaatt ctctgttgca tgagacgaga
      601 cgagttgtgt atgattaaaa gatgcaaagc aacgtagact taaaacgcct gtgggtctat
      661 gtcaggccgt atctaaaacc tttgaccctg tccgccgtac tcctgtcggc cactgcagtc
      721 attaaccttg cactgctgtg ggtgcttcgt aatgcattta atgacatcct cgtcgaacac
      781 agtcagaccg cgctatccgc caaagtcttg atcctgtttt ccctatttct gatccagagc
      841 attctctcca tgggtcacaa ctatttgatt acgtggatcg gtcagcgggt cgtaaccgat
      901 tttcgctgta agctgtttga acacctccag cgcctgtctt tgagtttctt cgccaagcgg
      961 cgaacgggcg aactgatctc tcgattcacg aacgacgtcg gaatcatcca gaacatgatc
     1021 gtgaacgttc cggtggacac ggtgaaacaa accgtgacgc tcctgggcgg catcgctatt
     1081 ctgttttacg tcaattggaa actctgcctg atggtcctga cccttctgcc tttgatcgcg
     1141 ctgacagccc gttacttcgg aaagaaactc cgacgtcttt caactcaaat tcaggatcag
     1201 ctcgccgact cttcaaccgt gttggaagaa gtcatttccg gaatccgcat ggtcaaatca
     1261 ttcgttcagg aagagtacga gctgcgtcgt ttcagccgcc agatcgaacg cgtgctggat
     1321 atcacgatgc agaagacgaa ggtcctggcc gtgttcgtcc ccgtcattac attcctcacc
     1381 atgggggcgg gggcgggtgt tatctggtac ggcggaaatc aggtcatcca gggacacatt
     1441 acgccgggag acctgttcgc ctttgcattg tatgccggac tcatgatcgg cccgtttgga
     1501 gcctttgcgc ggctcttctc acagattaaa gaggcccagg gtgcgacgca gcgggtgttt
     1561 gagattttag acacacaacc tctgatcctg gaccttccgg atgcgaaacc gatgccgacg
     1621 atccgcggac aggttgcatt ccatcaggtg agttttgctt atgatccgga catcccggtg
     1681 ctgaaggatc tctcattcga ggtggaaccg ggtcaggtcg tggcgctggt cggccccagc
     1741 ggatcgggga agacaacgct ggtaaatctc ctccaccggt tttatgatcc gaccgcgggt
     1801 tggattgaga tcgacgggca ggatattaaa aagattcagc tcagaaacct ctatacgcag
     1861 atcgctctgg tgccacagga aacgatctta ttcggtggaa cgatccggga aaatatcatg
     1921 tatggccgga ccgaggcttc ggaagaagaa atgatggctg ctgcaaaagc cgccaatgcc
     1981 catgacttta tcatggcctt cccgaaaggc tatgacacga tcgtgggcga gaaagggatc
     2041 aacctttccg gcgggcagcg ccagcggttg gcgatcgcgc gggcaatctt aaaaaacccg
     2101 cggcttctga ttctggacga agcgacttca tctcttgata atgagtccga gaagcttgtg
     2161 caggaggcat tagaccgcct catggagaac cggaccagct tcattatcgc ccatcggctc
     2221 acgacgattc aacacgcgga tcgtatcttc gtactggaca agggaaaact cgtggaagag
     2281 ggcacgcatg ccgaacttct cgaccgcaag ggactctacc atcatctata cacaatgaag
     2341 ctggctggag tagaggtata gttatctctc gcgcctctcc tctcctcgct cccctcttat
     2401 ctctttccgt tcctcccttc tctctctggc ctcagccttg aaatgcttcc ctctaaactc
     2461 tgggccgcgc tcatgatact ccttccaggc atgatcgcga aatgcatgga gcctctcaat
     2521 tttatatttt cttaagccga ccggaagccc tcggcgttcc acatgaaccc atggaccgcc
     2581 ataaccgcga gacccgtacc agtaaccgtc atgaaaatag tagtatcggc cgccgaggaa
     2641 aaaaagatcg tacggagttc caaccgccac gtatactcca gtctccggca tgattaccat
     2701 ctcgggtgga gcctccacca caaccggagg gggaacagga acaccgatat tgatattcac
     2761 acccacgccc gcataacttg acggcacggc ccaggccatt gctccgaaaa caatcaagaa
     2821 cataatcata ttttttttca tggctcctcc ttaattttct taaagattac actatcttaa
     2881 aataaccgaa aaggctcgat ctgtcaagca aatttccgat tcagcaagtt ttactcgacc
     2941 tgtttgatct gctcttcttg aagcgaggag ggagacccga tctcggctgc aaagattttt
     3001 gtacggtgga cagggtgggt cagcggaatt ccataaatgg ggatatggta ttcaaaatcg
     3061 agcgtgatcc gtttgttatg aacagccgca cccgtgccgc ccaagacacg gctaccaaac
     3121 gagctcgcgg cggtgtcttc gatccgcacc gacacctctt ccggcgtcag atggatgccg
     3181 tttttttcag cccgggcgat cacctcttgt ttaaaagatt cctctgtctg tcctcggacc
     3241 gtgagcatca gtgtgtcgag gtcatcgctg aacccctcgg agaccttata atattttaaa
     3301 acctgataac cggcgtggaa tataatcccg acgagcaaga ccaacaggat tttcttgatc
     3361 acgttttatt ttagctcctg gagcacttga tcgatatctt gaggcaacag cacgatctcg
     3421 attcgacggt tctgcgcctt tccctccttg gtgtcattgc tggcaacggg gtgattcatc
     3481 gcataaccgg ctgccgagag catcttggga tccagcttca ctttgtcttc gaggtatcgc
     3541 accacgttgg tggcacgggt catggacaac tcccagttcg ttggaaactt gctcataagt
     3601 ttcgggccga tcgggacgtc atcggtatat ccttcgacgc ggatttgctt atccttgacc
     3661 ttctttaata tctcgcccac acggtcaaat accttgagac cttcgggttt gatctccgca
     3721 ctgccggaat cgaataaaa
//
LOCUS       DATMKH010000281         1531 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_134253,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000281 DATMKH010000000
VERSION     DATMKH010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134253"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1415)
                     /locus_tag="VNV63_03415"
     CDS             complement(<1..1415)
                     /locus_tag="VNV63_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61700.1"
                     /translation="MAEGIYQLDNYELGYKAFVNRINKISGSTRPSISISRLNLHIPP
                     FLNFDNWATQHNTEERSTTVKSRRLWVANIGSGIYAYFDLDKGSTGPPTDWEQQVVKM
                     REVRAALGKNQRTETMDPLASPKSKPGGPKLAPTTPAKTTVIQRSTKDRPANYWSEQG
                     KEWEKKRGEWGKEFRTALKGRYRTYREKLQVEKKLGGKRSAPASEQTEVREYKQLMFW
                     SGLPGRFLGKIRFLLGSAWDKIIGIFEKMKERMHGVRKKISGTSETGFLATGWRKTLI
                     KILVKAAKVAVIKFIGDSFNFFVECFHSAMDKVWEKLQGELHEKFAEELCQARKFFED
                     SKERLENEWGISLKQLQELVEFIQDAKHWVDIATGLIQTIRLGIQAISCLTPPALGCL
                     WGLVAQIGISAGLDLLIGTQWFNDHILSPTIGELVRKYATPYYQKLINRALGDNLKEY
                     HCHIADERPLGSGFEVNDGLK"
BASE COUNT          304 a          442 c          388 g          397 t
ORIGIN      
        1 tccttcagac cgtcattcac ttcaaacccg cttccgagtg gcctctcatc ggcgatgtgg
       61 cagtgatatt ccttcaggtt gtctcccaga gcccggttga tcagcttctg gtaatacggt
      121 gtggcgtatt ttctgacaag ctctccgatc gtcggtgaga gaatatgatc attgaaccac
      181 tgggttccaa tcagcaggtc gagaccagcg ctgattccga tttgtgcgac caggccccac
      241 aagcaaccca gagccggagg cgtgaggcac gatatcgcct ggatacccaa gcgaatcgtt
      301 tgaatcaaac cggtagcaat gtccacccag tgtttagcgt cctggatgaa ttccaccagc
      361 tcctgaagct gctttagtga gataccccac tcattctcca gtcgctcctt gctatcctca
      421 aaaaactttc gtgcctgaca taactcttcc gcaaacttct cgtgaagttc gccctggagc
      481 ttttcccata ccttgtccat tgccgagtgg aagcactcga cgaaaaagtt gaagctgtca
      541 ccgatgaact tgatcaccgc cactttcgcg gccttcacca gaatcttaat gagagttttt
      601 cgccagccag tggccaggaa gcccgtctcg gacgttccac ttatcttctt gcgaacgcca
      661 tgcatgcgtt ccttcatctt ctcgaagatc ccgatgatct tgtcccatgc cgaaccgagc
      721 agaaaacgaa tcttgcccag gaagcggccc ggcaggccgc tccagaacat gagttgtttg
      781 tattcccgta cctcagtctg ctcagaggcg ggagcgctgc gtttgcctcc gagtttcttt
      841 tcaacttgca gcttctcgcg ataggtcctg taccggccct ttaaagcagt gcggaattcc
      901 ttgccccact caccgcgctt cttctcccat tctttcccct gctccgacca ataattcgcg
      961 ggacggtctt tggtggacct ctgaatgact gtggttttgg cgggggtcgt gggtgcgagt
     1021 ttcgggccgc cgggcttgga tttgggggag gccagcggat ccattgtctc tgtccgctgg
     1081 tttttcccga gggccgccct gacctcgcgc atcttgacca cctgctgctc ccaatctgtt
     1141 ggtggcccgg tcgacccctt atcgaggtcg aagtacgcgt agatcccgct accgatgttt
     1201 gcgacccaca agcgccggct ttttacggtg gtgcttctct cttcggtatt gtgctgggtg
     1261 gcccaattgt cgaagttgag aaacggagga atatgcaaat taagccgaga aatgctgatc
     1321 gagggccggg tcgatccgct gattttgttt attcggttga caaacgcttt atagccgagc
     1381 tcgtaattat cgagctggta gattccttca gccaccttcc acgcggataa gggtttgatc
     1441 tcccctaact ggatctccgc cgggaaattt ccgtcccaac gacggactcc actacgaggg
     1501 ccagaaattt tgggtgatga cttgacagca g
//
LOCUS       DATMKH010000282         1324 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4303760, whole genome shotgun sequence.
ACCESSION   DATMKH010000282 DATMKH010000000
VERSION     DATMKH010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4303760"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(288..1304)
                     /locus_tag="VNV63_03420"
     CDS             complement(288..1304)
                     /locus_tag="VNV63_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61701.1"
                     /translation="MPSTNEWKHALRTLGHPMPQRAERRIPSGLAAMYGSVSAPKLGQ
                     IKIISATGLFLTTEERWPVGEVISITLQREGPPGDNSELQVDVRARVASYGEDGVGLG
                     FVLPAGLNANLWEVMVNHADAQPETDDISFMFRVVRTLLFLHRLCPSGAQEVIHGLGK
                     ELDESRTRSAIEIALKAERLLAGEPDADGMHIHPQILKSILRHGSWSNDDLVQQLWAG
                     LLASSCSLEGTDESKRDFIELLLHVTDTQAPIFVAGCRKAGELMSGADGKALQEVIIS
                     PEEMIQITGLYDRFRTAREVSHLFNFGLFENVFDFSTYVPNDRFDITPSSRGLELFRV
                     CRVR"
BASE COUNT          317 a          382 c          318 g          307 t
ORIGIN      
        1 cggtccggct gatcttcttg atgcgtccca gctttgaggc cgagacaccg cgataccggg
       61 ctgcaagtcc ggacggagtt cgccgttcag gccttgggcc atccgggtac cctaatttgc
      121 gaagcctgtg tttccagtca atattcgtaa gcaccttcca aatcttccgc tggaatcaag
      181 ccgccaaaag tggtgctgac ttgcggacgc aactcgaccc tgagaagctg cttgtgttcc
      241 atgcgctcag aatagcgcaa aggaacgttc agagaacccg gcctgcgtca acgaactctg
      301 caaaccctga ataattccag gcctcgactt gaaggcgtga tatcaaaacg atcgttagga
      361 acataagtcg agaagtcaaa aacgttctca aacaaaccaa aattgaatag atgagatact
      421 tctcttgcgg tcctgaaacg atcgtataag ccagtaattt gaatcatctc ttcgggagag
      481 atgataactt cttgcaaagc tttcccatct gctcccgaca tcaactcccc ggccttcctg
      541 catccggcaa cgaagatagg tgcttgagtg tctgtaacat ggagaagcag ctcaatgaaa
      601 tcacgcttgg attcgtctgt tccctccaag gagcaggacg atgccaaaag tcccgcccat
      661 aactgctgca ccaaatcgtc atttgaccat gatccatgcc tgagaatgct ctttagaatt
      721 tgcggatgaa tgtgcatgcc gtcagcgtcg ggctctccgg caagtagtcg ttccgccttg
      781 agggcgatct cgatcgcact cctggttctt gattcatcca gctcttttcc cagcccatga
      841 ataacctcct gcgcgccaga gggacatagg cgatggagga acagtaacgt tctcaccacc
      901 cggaacatga acgaaatatc atccgtttcc ggttgagcat ccgcgtggtt gaccataacc
      961 tcccaaagat tcgcattcag gcccgcggga agcacgaacc ccagaccaac gccatcttcg
     1021 ccatacgatg cgacccgcgc gcgcacatcg acttggagct cggagttgtc tccgggcgga
     1081 ccttcccttt gcagtgttat tgagatcact tcaccaaccg gccagcgttc ctccgtggtc
     1141 aggaaaagtc cggtcgcact gattattttg atttggccca gcttcggagc cgaaacactc
     1201 ccgtacatgg ccgcaagtcc agacggaata cgcctttcag ccctttgggg cattggatgc
     1261 cccaaggtgc gaagcgcgtg tttccactca ttagttgaag gcaccttctt tatctcccac
     1321 taag
//
LOCUS       DATMKH010000283         1282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3341853, whole genome shotgun sequence.
ACCESSION   DATMKH010000283 DATMKH010000000
VERSION     DATMKH010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3341853"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="VNV63_03425"
     CDS             complement(<1..379)
                     /locus_tag="VNV63_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HXC61702.1"
                     /translation="MSKSMPFLNSHAYALMRIVVGFLFMWQGSQKLFSFPTPMPTGVP
                     AFIIYIAGPIELVGGTLVLIGLFTRWAAFFCSGQMAVAYWMAHGMKALLPIQNGGELA
                     IIYCFVFLFIATQGGGVWSVDAAL"
     gene            574..1239
                     /gene="msrA"
                     /locus_tag="VNV63_03430"
     CDS             574..1239
                     /gene="msrA"
                     /locus_tag="VNV63_03430"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012773961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HXC61703.1"
                     /translation="MKRFGLERIWIFAVIWIAIAAVIAPEIASIPARAAENPAASSTR
                     LEVATFAGGCFWCMVPPFAKLEGVVSVTSGYTGGHVKNPTYEQVSAGGTGHAEAVQMM
                     YDPQKVSYEKLLEVFWHNIDPTALDYQFCDHGHQYRSGIFYHTEAQKRLAEQSKSDIE
                     KSKPFKEPIVTEITQASAFYKAEEYHQDFYQKNPIRYKYYRYHCGRDGRLQELWGKAQ
                     TGH"
BASE COUNT          345 a          321 c          347 g          269 t
ORIGIN      
        1 ctagagcggc atcgacactc cagacacctc ctccctgagt cgcgatgaac agaaagacaa
       61 aacagtagat gatcgcgagt tcgccgccgt tctgtatcgg aagcagcgcc ttcattccat
      121 gcgccatcca atacgcgacg gccatctgac cgctgcaaaa gaatgccgcc catcgagtga
      181 agagcccgat cagcacgagc gttcctccaa ccagttcaat cggaccggca atgtagataa
      241 taaacgcggg aacgccggtc ggcatcgggg ttggaaaact gaacagcttc tgagaacctt
      301 gccacataaa gagaaacccg acaacgattc gcatcagcgc ataagcatga gaattgagga
      361 agggcattga cttcgacatg agaacacctc cagtttatga gttgttgatt tgtactttgt
      421 cgataagaac aagagacagt ctatcgttgt ccatcgtaaa agtaaagacc atgtgctccg
      481 attgattcag ttttgtgata aaatacaggt ctggagaaac ggatacggcc ggttcgatag
      541 gttgtatgtc tccgactccg aggagggatc cttatgaaga gatttggatt ggagaggatt
      601 tggatattcg cggtgatatg gatcgcgatc gccgcagtga tcgctcccga gattgcttca
      661 attcccgcgc gcgcagcaga aaaccctgca gcttcaagca ctcggctgga agtcgcaacc
      721 ttcgccggag gctgcttctg gtgcatggtg ccgccttttg caaagctgga gggagtggtc
      781 tcggtcactt ccgggtacac cggcggccat gtgaagaacc ccacttatga gcaggtctcg
      841 gcagggggga cagggcatgc cgaggcggta cagatgatgt acgatcccca aaaagtcagt
      901 tacgagaagc tgctggaagt cttctggcac aatatcgatc cgactgcgtt agattaccag
      961 ttttgcgatc acggccatca gtaccgcagc gggattttct atcacactga agcgcagaag
     1021 cgcctggccg aacagtccaa atccgacatc gaaaaatcaa agccctttaa agagcccatc
     1081 gtaacagaaa tcacacaggc ttcggctttt tacaaagcag aggagtacca tcaggatttt
     1141 taccaaaaaa acccgatccg ctacaaatat taccgctatc actgcgggcg agatggccgt
     1201 ctccaggagc tgtggggaaa ggctcagacg ggtcattgag ggaaggcggg atggtgcacg
     1261 acaatacaga tttcggttgc gg
//
LOCUS       DATMKH010000284         1021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_135369,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000284 DATMKH010000000
VERSION     DATMKH010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135369"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VNV63_03435"
     CDS             <1..346
                     /locus_tag="VNV63_03435"
                     /inference="COORDINATES: protein motif:HMM:NF021427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HXC61704.1"
                     /translation="RFHPTAAISGLTFARLSAQGRDTKMEILYRYLTGPEFRERVNAI
                     LRTFVGMQEQLEKEKRALIKQWGAREKQIETVIDGLSGMYGDLQGIIGSASLPEISNL
                     KLEDEDQEPRLL"
     gene            599..>1021
                     /locus_tag="VNV63_03440"
     CDS             599..>1021
                     /locus_tag="VNV63_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014392876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXC61705.1"
                     /translation="MPDHYDNPLGTCFEDLKYPYPTAFLNLHVEGQDVRMCFMDIQPP
                     KNPNGRTVLLLHGKNFWGAYWNDTIKFLTDRGYRVVVPDQIGFGKSSKPDIHYSFDFL
                     AENTAGLLDLLQIPKAVVVGHSMGGMLGIRFARLYPGRT"
BASE COUNT          236 a          258 c          276 g          251 t
ORIGIN      
        1 tcgcttccac cctacggcag caattagtgg cctaaccttt gccaggctat ccgctcaagg
       61 ccgggataca aagatggaga ttctttaccg ctatctaacg ggcccggaat tccgagaaag
      121 ggtaaacgcc atattgcgga cctttgtggg gatgcaagaa cagttggaaa aggagaaacg
      181 cgcgctgatc aaacaatggg gcgcgcgaga gaagcagatc gagaccgtca tcgacggcct
      241 ttccggtatg tatggtgatt tacaagggat tatcggcagc gcatccctcc ctgaaatctc
      301 caacctgaaa ctcgaagacg aggatcagga acccaggttg ttgtaggcgg aggtagacgg
      361 tagggacgag ctcgtgctgg tccgtttggt tctactttcg aagtccgcca gttcgatatt
      421 tcacaaaatt ctgcggtcga gcaggagctc gtccctatcg tccctggcgt tcccgctcgt
      481 tgtcatttgt catctgtcat ttgtcattca tcttcccgtc gtgacaagaa cacggatagg
      541 cttggcctgc ttcttattct taatactggc gggtccagcg gtagcgcagg agaaatcgct
      601 gccagatcat tacgataatc ccctcggaac ctgttttgag gacctgaaat atccgtaccc
      661 gactgctttc ctaaaccttc atgtggaagg gcaagatgtg cggatgtgtt tcatggatat
      721 ccagccgcct aaaaacccga atggacgaac ggttctgttg ctgcacggta agaatttttg
      781 gggagcttat tggaacgaca ccatcaagtt tctgaccgat cgcggctacc gggtcgtcgt
      841 tccggaccaa atcggattcg gaaaatcatc gaaaccggac attcattata gtttcgattt
      901 tctggcggag aacacggcag gtctccttga tctgttgcag ataccgaaag ccgtggttgt
      961 cggccattcg atgggcggga tgctcggaat ccgctttgct cgcctctacc cggggcgaac
     1021 t
//
LOCUS       DATMKH010000285         2293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2369845, whole genome shotgun sequence.
ACCESSION   DATMKH010000285 DATMKH010000000
VERSION     DATMKH010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2369845"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /locus_tag="VNV63_03445"
     CDS             complement(<1..751)
                     /locus_tag="VNV63_03445"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HXC61706.1"
                     /translation="MKDQETIKWRLWDTEAFQAARDLDKPIFLSISAVWCHWCHVMDE
                     ESFDHPEVIRRINTDFIPVRVDSDKRPDINSRYNMGGWPTVAILNSEGKVMAGGTYLP
                     LGQLLTMLASVKQEPNEIRQAPKETVVGADLRVCPGRPGPLGIPDGSRAAPTESAVQT
                     VGGFLERAFDSHFGGFGGAPKFPQPWAVELAFHLGLSTGEKKWLEMATLTLDNMRDGG
                     IYDAVEGGFFRYATRGDWDNPHYEKLLETNAR"
     gene            complement(811..1257)
                     /locus_tag="VNV63_03450"
     CDS             complement(811..1257)
                     /locus_tag="VNV63_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXC61707.1"
                     /translation="MKARYLGHIVFYVKDLERSLAFYRDLLGFKEVGRAFGGAAAALT
                     SGRTHHELLLIEVGDASRPPSGRRLGLYHIGIKIGDSLEELRSAKAELEHAGVTITGM
                     SDHTVSQSLYLTDPDGNEVELYVDADPAIWKKNPAAVLAPIKPLRL"
     gene            complement(1283..1666)
                     /locus_tag="VNV63_03455"
     CDS             complement(1283..1666)
                     /locus_tag="VNV63_03455"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HXC61708.1"
                     /translation="MLFIWLVLRPALKRPALDSQAQAVLSRVEERFRTVRWASLLTLL
                     GTGLFNLIREGGSARLESNWGGVLMIKLFFVFIVMGATIINDFLVTPTKTSPPSASRS
                     KDWLSGVILFLGLLIIFIGVYLGRM"
     gene            complement(1738..>2293)
                     /gene="recO"
                     /locus_tag="VNV63_03460"
     CDS             complement(1738..>2293)
                     /gene="recO"
                     /locus_tag="VNV63_03460"
                     /inference="COORDINATES: protein motif:HMM:NF014607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HXC61709.1"
                     /translation="ADILHSFQSIRENLEQVELASWMANLIKAFTAEEIQKPSLFQHL
                     LNCLEAIEKKNAPGLLPAYFELHLHKYVGYQPKLDRCIRCQGPLGKDECCFLPSAGGV
                     LCARCGDGSSEPILSPGAVALIQHLLKTDISVLNRLKPSLRQVYEVKEMLNVYDRYLL
                     EKNVNYGKYNPVKTDDSVRSFSKL"
BASE COUNT          578 a          645 c          560 g          510 t
ORIGIN      
        1 tccgggcatt cgtctccaga agtttttcat aatgcgggtt gtcccagtcg ccgcgtgtcg
       61 cataccggaa gaatccacct tcaacggcat catagattcc tccatcccgc atattgtcca
      121 gcgtgagcgt tgccatctcc aaccacttct tttcgcccgt ggacagtccg agatgaaagg
      181 ccagctcaac cgcccaaggc tgagggaact tcggcgcgcc tccgaaaccg ccgaaatggg
      241 aatcaaatgc ccgctcaaga aaaccgccaa cagtctgaac ggcagattcc gtaggggcgg
      301 cccgggaccc atcgggaatc ccaaggggcc ccggccgccc tgggcagaca cggaggtctg
      361 cccctacaac agtttcctta ggagcctgcc gtatctcatt tggctcttgc ttgactgagg
      421 ccagcatcgt cagcaactgc ccgagcggca ggtaagtacc gccggccatc actttccctt
      481 ccgaattaag tatggcaacg gtgggccagc cgcccatatt gtagcggctg ttaatatccg
      541 ggcgcttgtc gctgtccaca cggaccggga taaaatcggt gttgatccgg cggatcacct
      601 caggatgatc gaagctctct tcatccatga cgtggcacca atgacaccaa acggcagata
      661 tggatagaaa aatcggcttg tccagatctc gcgcggcctg gaaagcctcc gtgtcccaca
      721 atcgccactt tatcgtttct tgatctttca taacggcctc aacggccatt atagtcgtaa
      781 ttcatagagg tgttcaaggc aggagatgaa tcagagccgc aacggtttga tcggggccag
      841 aacggctgcc gggttcttct tccagatcgc cggatcggca tccacataca gctcgacctc
      901 attgccgtcc gggtccgtta gatacaggct ctggctgacg gtgtgatcac tcatcccggt
      961 gatcgtgacg cccgcgtgtt ccaactcggc cttcgcagag cggagttcct ccaaggagtc
     1021 cccgatcttg atcccgatat ggtacagacc aagccgccgc ccggaaggag gacgggatgc
     1081 gtcacccacc tcgattaata gcagctcgtg gtgggttcgg ccggaggtca gcgcggcggc
     1141 agcaccgccg aatgcccgtc cgacttcctt gaaacccaac aagtcgcggt aaaacgcaag
     1201 cgacctctcc agatccttca cataaaatac aatatgtccc aagtagcgcg ctttcattta
     1261 agtcctccaa tagcccatac ctttacatac gtccgagata gaccccgatg aaaatgatca
     1321 agaggcctaa aaagagaata acgccgctca accaatcctt cgagcggctc gcggaaggcg
     1381 gcgaggtttt agtcggtgta acgagaaaat cgtttattat agttgcgccc attacaatga
     1441 acacaaaaaa tagcttgatc atgaggacgc ctccccaatt cgattccaga cgggcggagc
     1501 cgccttcacg aatgaggtta aaaaggcctg tgcccagaag cgtcaaaaga ctggcccatc
     1561 ggaccgttcg aaagcgttct tccacgcggc tcagaaccgc ctgagcctga gaatcaaggg
     1621 ccggtcgttt cagtgcgggc cgaagcacaa gccaaatgaa cagcatcccg ccaatccaaa
     1681 tcatggctga gataacatga atccatagaa tcagcattgc aaacatagtg tcttgcctca
     1741 aagtttagaa aaggatctca cagaatcgtc tgtcttgacc ggattatatt taccataatt
     1801 aacatttttt tctagtagat agcgatcata aacattaagc atctctttaa cttcataaac
     1861 ctgtcttaaa cttggtttta accggttcag gacagagata tctgttttta agagatgttg
     1921 tatcaaagcc accgctcctg gacttaaaat cggctccgag ctgccgtctc cgcatcgagc
     1981 gcagagaaca ccgcccgcag atggaagaaa acagcattcg tcttttccca aagggccctg
     2041 acacctgatg catcggtcca gcttgggctg atagccgaca tacttgtgga gatgaagctc
     2101 aaaataggca ggcaacaatc ccggagcgtt tttcttctcg atggcctcca ggcaatttaa
     2161 aagatgttga aatagactgg gtttttgtat ctcctccgcc gtaaatgcct taatcagatt
     2221 ggccatccaa gaagcaagct cgacctgttc cagattttcg cggattgact gaaatgaatg
     2281 cagtatgtcc gct
//
LOCUS       DATMKH010000286         1165 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_777125,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000286 DATMKH010000000
VERSION     DATMKH010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_777125"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /locus_tag="VNV63_03465"
     CDS             <1..778
                     /locus_tag="VNV63_03465"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXC61710.1"
                     /translation="TQKEILREEHERLGLPLKGFHAREVARRVQEIELADGIICPSTF
                     VRQSFIDRGTPPDRVRTVPFGIQVSSGMEPGERPPDVFRVLFVGQINLRKGLRYLFEA
                     FRQFKHPNKELWIVGPRTEQSGIEDMTPPENTRFLGVLKGEDLARVYRTCHVFVLPTL
                     EEGLALVIGEALSYGLPVIATVNSGGADLFSDGSTGFLVPVRSPEAIADKLQLLADNP
                     DLLAELSARAVTRDKGIRSWENTCEILVKTLEDFIRMPKL"
     gene            775..>1165
                     /locus_tag="VNV63_03470"
     CDS             775..>1165
                     /locus_tag="VNV63_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61711.1"
                     /translation="MNEIPTDQLNARRLRQLIFGMVILLTFEGLARKAAPTEFRVPLF
                     FLKDFLTLAMAFYVFRMPMAPMIQSLWGGYMAMVVLLIPSILVTAWHDPLLAIFGAKQ
                     YLLYPIVGFAMFMAFENSGRAEIHAFFR"
BASE COUNT          247 a          304 c          317 g          297 t
ORIGIN      
        1 cacccagaag gaaattcttc gggaagagca cgagcgactg ggattaccac tgaagggatt
       61 tcatgcgcgt gaagtggcgc gacgggtaca ggaaatcgaa ctggcggacg gaattatttg
      121 tccatctact tttgtgcggc aatcgttcat tgatcgaggc actcctcccg atcgggtccg
      181 caccgtaccc ttcggcattc aagtgtcttc cggcatggaa cccggtgaac ggcctcccga
      241 tgtatttcga gttctattcg tcggtcagat caatcttcga aagggattgc gctatctttt
      301 tgaggccttc aggcaattca agcacccgaa caaggaactt tggatagtcg gcccaagaac
      361 agaacagtcg ggaatcgagg acatgacacc tcctgaaaac accaggtttc ttggcgtgtt
      421 aaaaggagaa gacctggccc gggtttatcg aacctgccat gtttttgtcc tcccgactct
      481 tgaggaggga ctggccctgg taatcggcga ggcgctttct tacggcctgc cggtcatagc
      541 cactgtcaac agtggtggag cggatctttt cagcgatggt tcgaccggtt ttttggttcc
      601 ggtccgctct ccagaggcca tcgcagacaa attacagctt ttggcggata accctgacct
      661 cctcgcggag ctatcggccc gtgcggtgac gcgtgacaag ggaatcagaa gttgggaaaa
      721 tacctgtgag atactcgtga aaaccctgga ggatttcatc cggatgccca agctatgaat
      781 gaaatcccga ccgatcagtt aaacgcccgc cggctcagac aactcatctt cggcatggtg
      841 atcctgctga cctttgaagg gctcgcgcgc aaggcggccc cgacggagtt tcgggtacct
      901 ctgttttttc tcaaagattt tctcacacta gccatggcgt tttatgtctt ccggatgccg
      961 atggcgccga tgatccaatc cctttggggc ggttacatgg ccatggtggt cttgttgatt
     1021 ccctctattt tggtgacggc gtggcatgat cccttgctgg caattttcgg cgccaagcag
     1081 tatctcttat atccgattgt cggatttgcc atgttcatgg cattcgagaa ttcgggacgg
     1141 gctgaaatcc acgctttctt ccgct
//
LOCUS       DATMKH010000287         1078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_136661,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000287 DATMKH010000000
VERSION     DATMKH010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136661"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..>1078
                     /locus_tag="VNV63_03475"
     CDS             195..>1078
                     /locus_tag="VNV63_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003276389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXC61712.1"
                     /translation="MNIATATPEQLVSYHSIRVNDLDIFYREAGPKDAPTILLLHGLP
                     SSSRMYQSLLESSLSEKYHLIAPDYPGFGHSSWPNPKEFAYTFDSLAKVMQDFTEQLH
                     LERYTLFMQDYGGPVGFRMALAHPEKIQAMIIQNAVAHEEGLSPLWAVRRAFWQDRAA
                     HEAEVRANLLSLEATRKRHLGTSPDSSQYNPDLWVDEYYFLNQPGQADIQLDLFYDYQ
                     NNVKSYPKWQQWLRDHQPPLLVLWGRYDTSFTVAGAEAYRRDDPHVEVHILDAGHFAL
                     DLQAKEIIRLIVDFMKNA"
BASE COUNT          257 a          320 c          241 g          260 t
ORIGIN      
        1 gtaccgtccc gcatatgcga ggcggtacac tagcactagc agagcccgcg cggaagcgct
       61 tccgatcatt ctgacctatg cgtatgacta ccattcgttg ctgcagaaac cctagtggaa
      121 ttaccggttg gcatcttgtc ctcacttcat tcatcaagat actctcatta atctcttccg
      181 gagtcttcct gactatgaac atcgcaacgg ccacacccga acagctcgtc tcttaccatt
      241 ccattcgggt caacgatctc gacatatttt atcgagaggc gggaccgaaa gacgcgccca
      301 cgattcttct tctccatggg ttgccgtcgt cctcccggat gtatcaatcg ctgctggaat
      361 ctagcctgag cgaaaaatat catctcatcg cgcccgacta tcccggtttc ggtcattcct
      421 cttggccaaa ccccaaggag ttcgcttata ccttcgattc tctcgctaaa gtcatgcagg
      481 actttacgga acaactccat ttggagcgtt acaccctctt catgcaggac tacggggggc
      541 ccgtcggttt ccgcatggct ttggctcacc ctgaaaagat tcaagcgatg atcattcaaa
      601 acgccgttgc ccacgaagaa ggattgtctc ctctttgggc cgttcggcgg gctttctggc
      661 aggatcgagc cgctcacgaa gccgaagtac gagccaacct gctatcactt gaagcaacaa
      721 ggaagagaca cctcggaaca tctcccgaca gctcacaata caatcctgat ctctgggtcg
      781 atgagtatta cttcctgaac cagcccggac aggcggacat tcaattggat ctgttttacg
      841 actatcagaa caacgtcaaa tcctatccaa agtggcaaca atggctacgc gatcatcaac
      901 ctcccctcct cgtgctctgg ggtcgttacg acacctcctt caccgtcgcc ggcgcagaag
      961 cataccgcag agatgatcct cacgtcgaag tgcatatcct cgatgcaggg cactttgcgc
     1021 tggatttgca agcgaaagag atcatccgat tgatcgtaga cttcatgaaa aatgcctt
//
LOCUS       DATMKH010000288         2057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3020527, whole genome shotgun sequence.
ACCESSION   DATMKH010000288 DATMKH010000000
VERSION     DATMKH010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3020527"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..1049)
                     /locus_tag="VNV63_03480"
     CDS             complement(150..1049)
                     /locus_tag="VNV63_03480"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HXC61713.1"
                     /translation="MIFRERTIRNSFWLFAAISVLFHLSLMSLLALRWPASQAVSHPK
                     PMFVSVIDPAELFKIPTGKETVLPQKPKDLPKGDLTALPRGMTAPQPSQLQSNPSSQS
                     KTSKSSPSAVPELSDTRPSTTAPLPDAQPSEKPPREAASSESSSASVPQPSLTPIPKQ
                     SVPGLPFADQKELDQLAKVFTEKEMPKRDTIAINTDDLRYLTYMLGLKRRIEFVWEYP
                     AAAIAAGIQGELLLNFTIRKDGQLTDVSLVRSSGYHVLDEEAIQALKSASPYAPLPES
                     WHQDRITITGNFIYYGESRRSIQ"
     gene            1155..>2057
                     /gene="rlmN"
                     /locus_tag="VNV63_03485"
     CDS             1155..>2057
                     /gene="rlmN"
                     /locus_tag="VNV63_03485"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HXC61714.1"
                     /translation="MATVDLKALSFSELEAFTQELGWPKYRAAQIWRWIYQKKMDDFS
                     QMTNLSKTDRDLLEERAFIGHLKVAEQNRSLDGTMKYLMELADGSLIEAVLIPGAPSG
                     NRNSRRLTLCISTQVGCTLDCVFCYTGTMGLKRNLRAHEIVDQVIVAQKSLAESQRLT
                     NIVLMGMGEPLANYREVIEALYRITSPNGLAIPPRRITLSTAGLIPQIKQFMETALKV
                     NLAVSLNAPNQALRDRLMPKVNQAYPLEELIAACRSVPLAPRKRITFEYVLLGGVNDS
                     PEHAKQLARLVRGLACKINLIPFNE"
BASE COUNT          488 a          534 c          586 g          449 t
ORIGIN      
        1 cgcaagttaa aaacgggccg gatggagtga cggtcggagc ggtcgcagat ttttgatgag
       61 cacaggcgct aagagagaaa aaagtgagca tcaggaagcc cgcccattgc ggggcgcctt
      121 gaagccctgt tgagcaggac agacggagat tactggatcg aacggcggct ttctccataa
      181 tagataaagt ttccggtgat tgtgatccgg tcttgatgcc atgattccgg taatggggcg
      241 taaggggacg ccgacttcag cgcctgaatc gcctcttcat ccaacacatg ataacccgag
      301 gatcgaacga gggaaacatc ggtgagctgg ccgtccttgc gaatggtaaa gttgagaagc
      361 aactctcctt gaattcccgc cgcaatcgct gccgccggat attcccagac aaactcaatc
      421 cgccgtttga gccctaacat ataagtgagg tagcggagat catcggtatt gatcgcgatg
      481 gtgtctcgct tcggcatctc cttttcggta aacactttgg cgagctggtc cagctctttc
      541 tgatccgcaa aaggaaggcc cggaacagac tgtttgggaa tcggggtcaa ggaaggctgc
      601 ggaaccgaag cggagctcga ttcggacgag gccgcttcac gcggaggttt ctctgacggt
      661 tgagcatctg gaagcggggc cgtcgtggag ggacgagtgt ccgagagttc cggcacagcc
      721 gatgggcttg actttgatgt tttggattga ctggacgggt tggattgcaa ttgacttggc
      781 tggggtgctg tcatgccgcg gggcagtgcg gtcaggtccc ctttcggaag atccttgggt
      841 ttttgaggca ggaccgtttc cttgccggtc gggatcttaa agagctcggc gggatcaatc
      901 acgctcacaa acatcggctt gggatgagac accgcctgcg aagcaggcca tcggagcgcc
      961 agcaagctca tcagagacag atggaagagg acagagatcg ccgcgaaaag ccaaaaggag
     1021 tttcgaatgg ttctctctcg aaaaatcatg cctttcctca atatacggaa tggctgatta
     1081 taccataagc agctcgggca atcctactat ctcagacctt gcccggatct tataagatat
     1141 gttatcgtga caatatggca acggttgacc tcaaagcgtt aagcttctca gaactcgagg
     1201 cgttcaccca ggaactcggt tggccgaagt accgggcagc gcagatttgg cgatggattt
     1261 accaaaagaa gatggatgat ttttctcaaa tgaccaatct ctccaagact gatcgggacc
     1321 tgttagagga acgtgccttt atcggtcatc taaaagtggc cgagcagaat cggtcattgg
     1381 atggcaccat gaaatacctg atggagctcg ccgatggatc gctgatcgaa gccgttctta
     1441 ttccgggggc cccttcggga aatagaaaca gccgacgcct cacgctttgc atatcgaccc
     1501 aggtcggctg cacgctggac tgcgtctttt gctacaccgg caccatggga ctcaaacgga
     1561 atctgagagc ccatgagatc gtggaccagg tgatcgtcgc acaaaaaagt ttagcggaat
     1621 cccagcgcct caccaatatc gtcctgatgg gcatggggga accgctggcc aactaccgcg
     1681 aggtgattga ggcactttat cgcataacca gcccgaacgg attggcgatc cctccacgga
     1741 gaatcacgct atccactgcc ggcctcattc cccaaatcaa gcagtttatg gagaccgctc
     1801 ttaaggtgaa cctcgccgtc tcgcttaatg cccccaatca agccttgcgt gatcggctca
     1861 tgccgaaggt caatcaggcc tatccgctcg aggaacttat cgccgcctgc cggtccgttc
     1921 cgcttgcccc gcgaaaacgg atcacgtttg agtacgtact cctcggcggc gtgaatgact
     1981 cgccggagca tgcaaagcag ctcgcccgac tggtgagggg gcttgcctgt aagatcaacc
     2041 ttatcccgtt taatgaa
//
LOCUS       DATMKH010000289         3240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1417590, whole genome shotgun sequence.
ACCESSION   DATMKH010000289 DATMKH010000000
VERSION     DATMKH010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1417590"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VNV63_03490"
     CDS             <1..186
                     /locus_tag="VNV63_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HXC61715.1"
                     /translation="PQSFYEKIGLQNGDVLQQVNGVEIKDPQSFLAVFQQLKEENNFS
                     LDLMRNSSKETFQYDIR"
     gene            173..1807
                     /gene="gspD"
                     /locus_tag="VNV63_03495"
     CDS             173..1807
                     /gene="gspD"
                     /locus_tag="VNV63_03495"
                     /inference="COORDINATES: protein motif:HMM:TIGR02517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HXC61716.1"
                     /translation="MTYDRAVALIRCSLILAFILISAEFSWAETPPQADSAAPIPAAP
                     ATTQMITMDFNNVDLPIFVKFVSEITGKNFVIDERVRGKVTVFSPTKISIEKAYQVLL
                     SVLELRGLTAVSQEGVIKIIPLNEMPLPERTINVYYLENANAEEMVKVLSGIIAKSGA
                     PTPPRPVSQPQPGPGGPVSLPSGPMAAAGEFEGLVLIAADKATNALIITASSKDYEKL
                     RDVIKKLDIKRKQVYVEAVIMEISMDHLRQIGAEIQAPLPTTLDAAGNSVLSNTLSPI
                     GGTNFGGLTTFASQGPVGLTGLAIGVVKGTFTFGGNTFLNVGGLLRALESDNDVNLLS
                     APQLLTSDNQKAEIIVAQNVPFPTGQSQTVGGNILTTIDRKDVGIILRLTPQILDNNT
                     VKLDVYQEISALTNTPQSISGVVVGPTTNKRSATSIVVVKDKQTVVLGGLVQDNLTVS
                     VNKVPFLGDIPVLGWLFKFHSREPVKTNLLIFLTPYIVKEPEDLTAIRSQKTEQMSRF
                     LEKEKIENRGERKEFLETINPPKPEPQAEPKQSPGP"
     gene            1816..>3240
                     /locus_tag="VNV63_03500"
     CDS             1816..>3240
                     /locus_tag="VNV63_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002624543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HXC61717.1"
                     /translation="MINKLEPLGQILQQKFSLSPDRMEEALRIQQEKGGRLGEILIRI
                     KAAKEEEVLEALGIQLSLSCWSTLEPHQLDPTLLAKVPIGFSKRHELLPVTQVNGRVV
                     VAIADPLNLFALDDLRIVLASDVSPVIVSAKKILHCINQVYDRAVTDSAEQVIEDMGA
                     ESLDRLASELVEPEDLLEATDEAPIIRLVNSLLFEAVKDRASDIHFEPYEQELAVRYR
                     IDGLLYNILTPPKRFQNSIISRIKIMANLDIAEKRLPQDGRIGIKIAGRAVDIRVSVI
                     PTAHGERVVLRLLDKGSRLVGLEEVGLDADQLRVMDQLIRMSHGILLVTGPTGSGKST
                     TLYGVLSKINSSDINIITIEDPIEYQIKGIGQIQVNPKINLTFANGLRSILRQDPDVI
                     MVGEIRDVETAEIAIHASLTGHLVFSTLHTNDAAGAISRLVDMGIEPFLVSSSLIAII
                     AQRLVRLICPECRQGYTPVADELIK"
BASE COUNT          784 a          872 c          881 g          703 t
ORIGIN      
        1 ccccagagct tttatgagaa gatcggcctt cagaacggag atgtccttca acaggtcaac
       61 ggtgttgaga tcaaagaccc gcagagtttc ctcgccgtat tccagcagct caaagaagaa
      121 aataatttca gcctggatct gatgcgaaac agcagcaaag agaccttcca gtatgacata
      181 cgatagggca gtagctctta tccgttgctc tttgatcctt gccttcattc tgatctctgc
      241 ggaattcagt tgggcggaga ccccgcctca ggcggacagc gcagcaccga ttcctgccgc
      301 ccccgcgaca acccagatga tcacgatgga cttcaacaat gtagatctgc cgatcttcgt
      361 gaagttcgtc agcgagatca ccgggaagaa ttttgtgatt gatgagcgtg tccgtgggaa
      421 ggtgaccgtt ttttccccca ccaagatttc tatcgagaag gcgtatcagg tcctgctttc
      481 ggttctggag ctcagaggcc tgaccgcggt gtcgcaagag ggtgtgatca agatcatacc
      541 gctcaatgaa atgcccttac cggaacggac gatcaatgtc tattatctcg agaacgccaa
      601 tgccgaagag atggtcaagg tcttaagcgg gatcatagcc aaatccgggg cacccactcc
      661 accgcggccg gtttcccaac cgcaacccgg accgggtgga ccggtgtcgc taccctccgg
      721 accgatggcc gcggccggag agttcgaagg gctggtcctg atcgcggcag ataaagcgac
      781 caatgccctg atcatcacgg catcctccaa agattatgag aagttgaggg atgtgatcaa
      841 aaagcttgat attaagcgta agcaggtata tgtcgaggcg gtgatcatgg agatcagcat
      901 ggaccacctc cgtcagattg gagctgaaat ccaggcgccg ctcccgacca cattagatgc
      961 ggcagggaat tcggtactca gtaacacact gtcacccatt ggggggacca attttggcgg
     1021 gctcaccacc ttcgcgagcc agggaccggt tggcctgacc ggccttgcga ttggtgttgt
     1081 gaaaggaacc tttacattcg gaggtaatac tttcttaaat gtcggcggcc tcctgcgtgc
     1141 cttggaatcg gataacgacg tgaatctgct atcggctccc cagcttctca caagcgataa
     1201 ccagaaagcc gagatcatag tggcacaaaa tgtgccgttt ccgaccggtc agagtcagac
     1261 tgtgggaggg aatattctaa ccacgattga tcggaaagat gtgggcatta ttctcagatt
     1321 gacgcctcag attctggata acaacacggt caagctcgat gtgtaccagg agatttcggc
     1381 cctcaccaat acgccgcaat ccatcagcgg tgtcgtggtg ggtcccacta cgaacaaacg
     1441 gtcggcgaca tcaatcgttg ttgtcaagga caagcagacg gttgtgttgg gaggcctggt
     1501 tcaggacaac cttaccgtca gcgtgaataa agtcccattt ctgggagaca tcccggtttt
     1561 ggggtggtta ttcaagtttc attccaggga gcctgtaaag acaaaccttc taatctttct
     1621 taccccctat atcgtcaaag aaccggaaga tctgaccgca atccgttccc agaagacaga
     1681 gcagatgagc aggtttctag agaaagaaaa gatcgagaac cgaggagaac ggaaggaatt
     1741 cctggagaca atcaatccgc ctaaaccgga gccccaggcg gaacctaagc agtcacccgg
     1801 gccgtgatcc aaccgatgat caataagctg gaaccactgg gtcaaatcct ccaacagaaa
     1861 ttttctctgt cccctgatcg gatggaagag gcacttcgta tccagcaaga aaaaggcggc
     1921 cggctgggag agatcctgat tcgcatcaag gcggccaagg aagaagaggt tctcgaagcg
     1981 ttgggcatac agttgtcgct ttcatgctgg tcgacgcttg aacctcatca actggacccc
     2041 accctcttgg ccaaggttcc catcggcttt tccaaacggc atgagctcct gccggtgaca
     2101 caagtgaacg ggcgggtcgt cgtggccatc gcggatcctt taaacctctt tgcgctggat
     2161 gacctccgaa tcgtcctggc cagcgatgtg tcgccggtta tcgtatcggc caaaaagatt
     2221 cttcactgca tcaatcaggt gtacgaccgg gccgttacag acagcgccga acaggtgatc
     2281 gaggacatgg gggccgaaag cctggatcgc ctggcaagcg agctggtaga acccgaagac
     2341 ctccttgaag ccacagatga ggccccgatc atccggcttg tcaactcact cctgtttgag
     2401 gctgtcaagg atcgtgcgag cgacatccat ttcgagccgt acgagcaaga gctggcggtc
     2461 agatatcgga ttgatggtct gctctacaac atcctgacgc ctcccaagcg atttcagaac
     2521 agcattattt cccggatcaa gatcatggcc aatctcgata tcgctgaaaa acgcttgcct
     2581 caggacggcc ggatcgggat caagatcgcg gggcgggcgg tggacatccg tgtctcggtt
     2641 atcccgactg ctcacggtga acgtgtggtc ctgcggcttc tcgataaggg gtcgcggctt
     2701 gttggcttgg aagaggtcgg actggatgcc gatcaactcc gggtcatgga tcagctcatc
     2761 cgcatgagcc atggaatcct cctggttacg ggtccgaccg ggagcggtaa aagcaccaca
     2821 ctgtacggtg ttctttccaa gatcaactct tccgacatca acatcatcac gatcgaggac
     2881 ccgatcgagt atcagatcaa aggaatcgga cagatccagg tcaatcccaa aatcaattta
     2941 acttttgcga acggtctccg atccatcttg cgtcaagatc cggatgtgat catggtgggg
     3001 gagatccgtg atgtggagac ggccgagatt gcgatccatg cctcgctcac cggccacctg
     3061 gtcttctcca cgctccacac gaacgatgcg gccggggcga tctcccgact cgtggatatg
     3121 ggaatcgaac ctttcttggt ctcatcctcg ctgattgcga tcatcgccca gcgtctggtg
     3181 cggcttatct gccctgaatg ccggcagggc tacactccgg tcgcggacga attaataaag
//
LOCUS       DATMKH010000290         1210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_137809,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000290 DATMKH010000000
VERSION     DATMKH010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137809"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="VNV63_03505"
     CDS             <1..611
                     /locus_tag="VNV63_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXC61718.1"
                     /translation="HGISLIRKFLNIGYENVVLRAFEFKSPLIAGPDRSGPPSEEKKI
                     ESLQTIAWLQFGQKLAVFDFTGDQYFSWIRSQRLLIRGERGEINNLEGCYLDDFRRPV
                     RIRFERNDAGQTGNLEGYYHKGYGAGGEDWYQNPFAPGRLSDDEIAVATCLELMRRYL
                     ETGKSFYSLAEASQDHYLSLMIGEAARRARTLESSYQVWQQS"
     gene            complement(857..>1210)
                     /locus_tag="VNV63_03510"
     CDS             complement(857..>1210)
                     /locus_tag="VNV63_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dehydratase"
                     /protein_id="HXC61719.1"
                     /translation="EAFNITNGDFNRWENIWPRLAEFFKMEYAPPQQFPLALFMSDKE
                     RLWNTLVKKYDLLGYSFQEAASWPFGEAIFNLGYDVMADTTKARRFGFFEWVDTEEML
                     LRLLAQFQKMRFIPS"
BASE COUNT          301 a          315 c          328 g          266 t
ORIGIN      
        1 accacggcat ttccctgatc cgaaaatttc tgaacatcgg ttatgagaac gttgtgctgc
       61 gggcgttcga atttaaatcg ccactgattg cgggtccgga tcgctcaggt cccccatcgg
      121 aagagaagaa gatagaatcc ctccagacca tcgcttggtt gcaattcggt caaaagcttg
      181 cagtctttga tttcacgggc gaccaatatt tctcatggat ccgttcccag cgtctgctta
      241 ttcgcggaga acggggagaa atcaacaacc tggaaggttg ttacctggac gatttccgta
      301 ggcccgtcag gattcgattt gaaagaaatg atgcgggtca gacaggaaat cttgaagggt
      361 attaccacaa gggctacggc gcaggaggcg aagattggta tcaaaatcca tttgcgcccg
      421 gccgcttgtc tgacgacgaa atcgccgtgg caacctgtct cgaattgatg aggcgctatc
      481 tggaaacagg gaagtccttt tacagtttgg cagaggcctc tcaagaccat tatctgagcc
      541 tcatgatcgg ggaagcggcc cgaagagcaa gaacgttgga gagctcgtac caggtctggc
      601 agcaatcttg aaactggcga aagagacggc gcatcgtggt cagcgaaacg tcccgctttg
      661 gcctcttcaa cttcaactgc ggacgccccg gagcaagccg ggacggcatg cctacagtaa
      721 gtgaggcgtc ccgctttacc tcttcaaccc cggagggaaa acccgggtaa gccgggacgg
      781 cttacttacc ggctggggaa gcacagcgtg acgctttgct aatgtacggc gacacgccga
      841 cacctttttt cacccgtcag gatggaatga atcgcatctt ctggaactga gcaaggagtc
      901 gaagaagcat ttcctcggta tcgacccatt caaagaatcc aaaacgccga gccttggtgg
      961 tatccgccat gacgtcgtaa ccgagattaa agatcgcctc tccgaacggc catgaagcag
     1021 cctcctgaaa ggagtaaccg aggaggtcat acttctttac gagagtgttc cagagtcgtt
     1081 ctttgtctga cataaacagc gcgaggggga actgctgcgg cggcgcatac tccatcttga
     1141 agaactccgc cagccgcggc cagatgttct cccaacgatt gaagtctccg tttgtaatat
     1201 taaatgcctc
//
LOCUS       DATMKH010000291         1590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3644213, whole genome shotgun sequence.
ACCESSION   DATMKH010000291 DATMKH010000000
VERSION     DATMKH010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3644213"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="VNV63_03515"
     CDS             <1..511
                     /locus_tag="VNV63_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61720.1"
                     /translation="DVMNRYDLLPLYQRRLHMPTVFLTGDGPTPYGPNSSILNRLIVC
                     QLQRRGAFQYIPGRARIARVFSEDTYLKRDVDFPDGTTRTFHRVIERHGPRKAIDDPC
                     FGTFRKGFHALKERWDKLFVIGDHTREKQWEVNYFGTNPAAQIDRRERAFEARVQEIG
                     IRIRRLSVT"
     gene            539..>1590
                     /locus_tag="VNV63_03520"
     CDS             539..>1590
                     /locus_tag="VNV63_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61721.1"
                     /translation="MNYQFEKLSVLTGKLSKLRVSFTSEAGIVGEFKLDADADRHGIK
                     WDPDNREPDSGTLEERIHCMSKLSGYLEFSAPLGPGSTVTFGFSLKIISGNALSKWEF
                     YQMYAPEKRRHLSGDDLGNEIEYLARIIWIPVEKLKVKIIFPSRVSTPPFLTLFRYND
                     PEKIPGSAVLDDEGVLHMSPPSGSDLHPKSEQGRNSWSRVDDPLLTEAGLTTLSNTAA
                     QTWELTVSKPLIGTIYSLDWILDSPSPDLDSLAEEARKFRARLLVHRQQRLAEGPGLP
                     GVRECFQELRKEVWKVFSPEPGEPFEVSVMTYDESNRVLVPIEGMVGEEEFPKNLWDT
                     AIPFGLGLAGSAFKEA"
BASE COUNT          363 a          438 c          423 g          366 t
ORIGIN      
        1 tgatgtgatg aaccgttatg acctccttcc cctataccag cgccggttgc acatgcccac
       61 tgtctttctt acgggagatg gcccgacgcc ctatgggccg aattcatcca ttctcaatcg
      121 gcttatcgtg tgccagctgc agcgccgcgg agcgtttcaa tatatacccg gacgagcaag
      181 aattgcgcga gtattttccg aagacactta cctcaagaga gatgtcgatt tccccgacgg
      241 taccacacgc acgttccatc gtgtcattga gcgtcacggc cctcgcaagg ccattgatga
      301 tccgtgcttt ggaaccttta gaaagggttt ccatgcgttg aaggaacgct gggacaagct
      361 tttcgtcatc ggcgaccaca ctcgcgagaa acaatgggaa gtcaattact ttggtacaaa
      421 tcccgctgct cagatcgatc gccgcgaacg ggcctttgaa gcgcgcgtgc aggaaatcgg
      481 tattcgaatt cgccgactct cggtaacata gaagctcgac aactccggca gctccacgat
      541 gaattatcag ttcgagaagc tctccgtcct taccggcaag ctctccaaac tccgtgtttc
      601 gttcacttca gaagccggga ttgtgggcga attcaagctg gatgcagacg ccgaccgcca
      661 tggtatcaaa tgggatcccg ataatagaga accagattct gggaccctcg aagaacgtat
      721 ccattgcatg agcaagttga gcggctatct tgaattttct gctcctctcg gacccgggtc
      781 gaccgttact ttcgggttta gcctcaagat tatcagcggg aacgctctat cgaagtggga
      841 gttctaccag atgtacgctc ctgaaaagcg ccggcatctg agtggcgatg acctcggcaa
      901 tgagatcgag tatctcgcaa ggatcatctg gattcccgtc gagaaactca aagtgaaaat
      961 tatcttcccg agccgtgttt ccacgcctcc gtttctgaca ctgttccggt ataacgaccc
     1021 tgaaaaaatc ccagggagcg cggtcctcga tgacgaaggg gtccttcaca tgtcccctcc
     1081 gtccggatct gatctgcatc cgaagtcgga gcagggcaga aattcctggt cgcgtgtaga
     1141 cgatcctctg cttaccgaag cgggactgac cacgctgtcg aacactgccg cccagacatg
     1201 ggagctgaca gtctccaaac cgcttatcgg aacgatctat tctctggact ggatcctcga
     1261 tagcccgtcg cccgacctgg acagcctggc cgaggaggca agaaaattcc gcgctcggct
     1321 tttagtgcat cgtcagcagc gcctggccga agggcccggc ctgcctggag tcagggagtg
     1381 cttccaggag cttcgaaagg aagtatggaa ggttttttcg ccggaaccgg gagaaccctt
     1441 cgaggtttcg gtcatgacct acgatgaatc gaaccgggtt cttgttccca tcgaaggtat
     1501 ggtaggagaa gaggaattcc ctaagaatct atgggataca gcgattccct ttggtctcgg
     1561 tctggctgga tctgctttca aggaggccga
//
LOCUS       DATMKH010000292         6231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_138823,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000292 DATMKH010000000
VERSION     DATMKH010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 6231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6231
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138823"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(723..1517)
                     /locus_tag="VNV63_03525"
     CDS             complement(723..1517)
                     /locus_tag="VNV63_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61722.1"
                     /translation="MSSIDLAGKFSFLTGGSIALLCLLALLDRDVWAYRPFVSTDAGV
                     ADFQEAEIELGYFNLERSQGQDTFIVPSTVLNYGVVHRLEAVVQFNIETPTNENARLV
                     DSELSLKGVLKEGILQEKEGASVAIEAGLLLPSMVQGERRFGFEGVGILSQRLFPFIL
                     HINLGGGVDQANTNPFFIWGVIGELPATPKFRFVGEVNGENHEKEPMDSSGLLGFIWQ
                     SPWPNLSLDAGIRRGISKASPDWQFTAGLTIGFFMPSVWTSHTATF"
     gene            complement(1589..1810)
                     /locus_tag="VNV63_03530"
     CDS             complement(1589..1810)
                     /locus_tag="VNV63_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61723.1"
                     /translation="MRNFAWSLTLFAMGLSLIAAAPGSEEAPPAFDNATNGFVDAATY
                     GGESSLERKKFLALTDRQKRQLATFLRSL"
     regulatory      1959..2140
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            2256..3179
                     /locus_tag="VNV63_03535"
     CDS             2256..3179
                     /locus_tag="VNV63_03535"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HXC61724.1"
                     /translation="MRCFRFALLFIFLLWSAIACLLDRPAHAESFQVTDALGRTLTFS
                     KTPRRIVSLAPSITETLFALGVDQEIIGVTTSCDYPPQAKLKPKVGGLTTPNLETLVA
                     MKPDLVISVKGLHKFELVSEMDRLKIPFYVSDPSTVKKILQEIRVIGRLTGRAKAGEQ
                     LSETMEKRIEAVEAKVRGHQRPAVLYVLWGDPLMTVGPGSYIEELIRISGGRGIVPEG
                     RPAYTQLNMEEVLAEDPQVVIFASEMGDDTVAAERQRWLRWTTLSAVKEHRLYTVDSN
                     LLHRPGPRLVDGLEALARLIHPELFPDQSAK"
     gene            3182..4204
                     /locus_tag="VNV63_03540"
     CDS             3182..4204
                     /locus_tag="VNV63_03540"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HXC61725.1"
                     /translation="MRSMTVQRWLYINGLFGLILIGAILIALLMGAEPISLSEMARIL
                     EGVPHQADSAKAAILLQVRLPRILLAGLVGGILALCGVVFQALLRNPLADPFILGVSS
                     GAALGAYLAMLFGLQSTVLGFTAMPLMAFAGGLMSIWFVYLIAKVDGQLPITGLLLAG
                     VVVNAVLSALILFITSVLDAGKVMNILVWVMGNIGSLDYRIIGTLAFYTLISGVLLLM
                     TAKPLNLLTLGEESALTLGLPVERVKKLLLVLTTFLTGAAVSVSGIIGFVGIIAPHAA
                     RMLVGPDHRVLLPASALVGASFLILADTIARTALGPTEIPVGVLTALCGGPFFLILLK
                     TKKMHG"
     gene            4210..5001
                     /locus_tag="VNV63_03545"
     CDS             4210..5001
                     /locus_tag="VNV63_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009382025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXC61726.1"
                     /translation="MTELYQVQELSFRYGAVPVLQGIAFATRAGEFIGIIGPNGSGKT
                     TLLKLLSAILKPNTGKIEFGGMDLRSWNRHDLAQKMAVVPQETAFLYTYTVLEIVLMG
                     RAPFTSLFGFDRPEDLRIALESLKQVGLLDMADRSIQTLSGGERQMVVIARALTQQPE
                     VLLLDEPTAYLDIRHQQKIYNLLTRLNKEQGLTVVVVSHDLNLAGQYCHRLIMLSDGQ
                     TVADGTAEKVLTKERIEAVYGCQVLIDRHPMTGRPRMTLVPHDAA"
     gene            4991..5719
                     /locus_tag="VNV63_03550"
     CDS             4991..5719
                     /locus_tag="VNV63_03550"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HXC61727.1"
                     /translation="MRHKQRKNRWPMAASVLVHSLFIGLALGFAAFGTRIVLPDAMMV
                     TIVSSIKGNSEANQIVQKTDSRSQIIAEVNEAKPKQFEEEKTHDTPLSIEPAEQADQA
                     EKTEETPAASPPSGDQGQTETVELNGGGPNSTGAEFVSTTSEDALAKFLLQVRQTIER
                     HKQYPRRARFQGIEGTTQLRFRILPTGEPTEIQVVQSSQSAILDEESVAAVKRVTRFP
                     QPPVKSPMGILIRLPFVFHLEEGG"
     regulatory      5724..6002
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6035..>6231
                     /locus_tag="VNV63_03555"
     CDS             6035..>6231
                     /locus_tag="VNV63_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61728.1"
                     /translation="MQTRLATVVILMGTLIGMTTGLWAEEAPEEATAEAVVVTATKTE
                     TPITEVASSVYVIDSKTIEDK"
BASE COUNT         1485 a         1563 c         1705 g         1478 t
ORIGIN      
        1 atctaccact acaacaacta cccttaaagc tgacgcattc actaccaacg tgcagttcca
       61 ctaatcggcg aacggctcgg cgggcggctg gagcggggcc aatatccttg gcgcctcggg
      121 taaaatcaca attggcccgc aagcgatgga aggaaatctg attgtcaacc ccggtgacac
      181 cctacacgtg ggctatgact tcacgatgcc aggccagcac ccgaccgtga cgcttgtctt
      241 tcagcaaacc actgtcacgt tccaggcgct ctgtgcttca ggaacgggcg gaggccgatc
      301 gtggtcaaga tcctggacgg cagctacgct gatccacaga acagccccaa ctggtatccg
      361 agtggacagc agaacgtttc ctctgtgtat caaggctcaa tcaccgtccc agatctctgc
      421 ggtggtgggc cactgagcct caggcaaggc gcaacgttta gcgttactgg gggcagtata
      481 taggcgccaa cgatctcggt cctgcggaat caactcggaa gtgtatcaag ccgaggagcc
      541 ggcgcacttc tggaagacaa cattccatct aacaatgtgc ttcagatttt actttggtgt
      601 caccagccgg accgaataaa gggggcaaag atcaggtttg aacattattt tagaagagct
      661 gaggaatcac agaacgttca gctctaactg gatgtggata aggtattact ccagcatggt
      721 gtttagaaag tagcagtatg ggaggtccac actgaaggca tgaagaaacc gattgtcagc
      781 cccgctgtga actgccagtc gggagacgcc ttactgatcc ctcttctgat tcccgcatcg
      841 agagagaggt tcggccaagg agactgccag ataaacccga gaagaccgga actgtccata
      901 ggttcttttt catgattttc tccgttaacc tctccaacga acctaaattt cggagtggca
      961 gggagctctc cgatcactcc ccaaataaag aacgggttcg tgttcgcctg atccacacca
     1021 ccgccgagat tgatgtgtaa tatgaatggg aaaagccttt ggctcagaat cccaacccct
     1081 tcaaagccga accttctctc accttgaacc atcgagggca agaggagtcc tgcttcgatg
     1141 gcaacgcttg cgccttcttt ctcttgtagg ataccctcct tgaggacacc cttcagagac
     1201 agttctgaat caacgagccg ggcattttcg ttcgttggag tctcgatatt gaactgaaca
     1261 acagcttcca ggcgatggac gactccataa ttcaacaccg tgctcggaac gatgaatgtg
     1321 tcttgcccct gagaccgttc gaggttaaag tagcccagtt cgatctcggc ttcctgaaaa
     1381 tcagccacgc ccgcatcagt cgagacgaat ggccggtatg cccagacgtc gcggtcaaga
     1441 agtgcgagga gacataacaa cgcgatactg ccacccgtga ggaaggaaaa ctttcccgct
     1501 aagtctatag aggacattct ctttttgtgc cccaggcttt ctgcccgtgg tccgggtcct
     1561 gtggcggggg ttggggaatc ctcgagcctc acaacgatct taagaatgtg gcaagttgcc
     1621 ttttctgacg atccgtcaga gcaagaaact tcttccgttc cagggacgat tctcccccat
     1681 aagttgctgc gtccacgaag ccattcgtag cgttgtcaaa cgccgggggt gcttcttctg
     1741 agccgggcgc agctgctatg agcgataggc ccattgcgaa cagcgtgaga gaccaagcaa
     1801 agtttctcat agtcacctcc cagagtttgt agagagtttt aaaaacgatc gtagggtctt
     1861 tttcgatcct aaagaggtta ccctaaagct tttcaaaaat caattcgtag atgcgccaaa
     1921 attttcttga cttttattct ctcatcccgt ataaagagag ccacagattt gttctaggtg
     1981 ctttgcttaa aagggaatcc cgtgtaaaac gggagcggtc ccgccgctgt aagtggggac
     2041 gaggcttgcc acgagtcact gtccgcttca aatggatggg aagacgcagg ctgagaatga
     2101 tccacaagcc agaagacctg cctagatgat aaagctcatg accctttcgg ctgaatgggg
     2161 agcatgggcc cgccttcagg gtgaagaagc agggtgcaat cctcgaggtc cttttagtta
     2221 gggctttgag gatttttatt ttttgaggtc gcaccgtgcg ctgttttcgc tttgctctgc
     2281 tttttatttt tctgctctgg tctgcgatcg cctgcctgtt ggacaggccg gcccacgccg
     2341 aatcttttca agtcacggat gccctcggac gcacgctgac tttttccaag acgcccagac
     2401 ggattgtctc tctcgctccc agcatcactg aaactttgtt cgcgcttggc gttgatcagg
     2461 agatcatcgg cgtaactact tcttgcgatt atccgcctca ggcaaagcta aagcccaagg
     2521 tcggaggcct gacaacacca aacctcgaaa ctctggtagc catgaagccg gacctggtca
     2581 tcagtgtcaa aggccttcac aagtttgagt tggtctcaga gatggaccgt ctcaaaattc
     2641 cgttctacgt ttcggaccca agcacggtga aaaaaatcct ccaagagatc cgcgtcatcg
     2701 gtcggttgac gggaagggca aaggctggag aacagttgtc tgaaacaatg gagaagcgga
     2761 tcgaggcggt tgaagcaaaa gtccggggac atcaacggcc agcagtcttg tatgttctct
     2821 ggggtgaccc gcttatgacg gtaggaccgg gaagctatat agaggaattg atccggatct
     2881 ctggaggaag gggaatcgtt ccggagggcc gaccggccta cacacagttg aacatggaag
     2941 aggttttagc cgaggatccg caagtcgtga tcttcgcttc cgaaatgggg gatgacacgg
     3001 tcgcagccga gcgtcaacgc tggcttcgat ggacgactct gagcgcagtc aaggagcaca
     3061 ggctttatac cgtagacagc aatctgctcc atcgccccgg gccccggctg gtggatgggc
     3121 tcgaggctct ggctagattg attcatccgg aacttttccc cgatcaatcg gcaaaataaa
     3181 tatgagatcc atgacggttc agcgatggtt gtatattaac ggtctcttcg ggttgattct
     3241 catcggggcc atattgatcg cccttcttat gggggcggag ccaattagcc taagcgagat
     3301 ggcccggatc ttggagggcg tgccgcatca agccgattcg gcgaaggcgg ctatactgct
     3361 tcaagtccga cttccccgta ttcttctggc cggactggtc ggtggaatcc tggctctatg
     3421 cggcgttgtc tttcaggctc tgcttcgaaa tccgctggcc gatcctttta ttttaggagt
     3481 ctccagcggt gccgccttgg gcgcgtatct cgccatgctg ttcgggcttc agagcactgt
     3541 tttaggattt accgcgatgc ctctgatggc ctttgcaggc ggactcatgt ccatctggtt
     3601 tgtgtacctg attgcaaagg ttgatggaca gcttccaatc accggtctct tacttgccgg
     3661 ggttgttgtt aatgcggttt tatcggccct gatccttttc attacctcgg tgttggatgc
     3721 gggtaaggtg atgaatatcc tggtctgggt catgggcaat atcggaagtc ttgattaccg
     3781 gataatcgga acccttgcgt tctacaccct gatcagcggc gtgttgcttc tgatgacggc
     3841 taagccgtta aacctcctga cgcttggcga agagtctgct ctcacgttgg gccttccggt
     3901 ggagcgggtt aagaaattac tgttggtgct cactaccttc ttgacaggcg cggcggtgtc
     3961 agtcagcggc attattgggt tcgtcggaat cattgctcct catgcggcgc ggatgctcgt
     4021 tggtccggac catcgtgttc tgcttcccgc ctcggccctg gttggagcaa gttttttgat
     4081 tcttgcggac acgattgcca gaaccgccct gggtccgacc gagattccgg ttggcgtgct
     4141 gaccgcgttg tgcggcggcc cttttttctt gatcttgctt aaaaccaaaa agatgcatgg
     4201 atagaaccaa tgacagagct ctatcaagtt caggaattga gttttcgtta cggtgccgta
     4261 ccggtacttc aaggaatcgc gtttgcgacc agggcgggag aattcatcgg catcatcggg
     4321 cccaacggtt ccggaaagac gacactgctt aagttacttt cagcaatact gaaacccaac
     4381 acggggaaaa tagaatttgg gggaatggat ctccggtctt ggaaccggca tgatctggcg
     4441 caaaaaatgg cggtggttcc gcaggaaaca gcttttcttt atacgtacac tgtattggag
     4501 atcgttctca tgggccgagc gccttttaca tctcttttcg gttttgaccg gccggaagat
     4561 ctccggatcg cccttgaatc gttgaagcaa gtcggtttgc ttgacatggc ggatcgttcg
     4621 attcagacgc tttcaggtgg ggagcgccag atggtggtca tcgcccgtgc cttgactcag
     4681 cagccggagg tcttgttgtt ggacgagcca accgcttacc tcgatatacg tcatcaacag
     4741 aagatttaca atcttcttac aaggttgaat aaggaacaag ggctcaccgt tgtagttgtc
     4801 tcccatgatc tcaacctcgc cggccagtat tgccacaggc tgatcatgct ctctgatggg
     4861 cagaccgtag cggatggtac tgctgaaaag gttttgacga aagagcggat cgaagcggtg
     4921 tacggttgtc aggttctcat agaccgccat ccgatgacgg gccgtccgcg tatgacattg
     4981 gtgccacacg atgcggcata aacagagaaa aaaccgctgg cccatggcgg cctcggtgct
     5041 tgtccattct ttattcatag ggcttgcctt agggtttgct gcctttggaa cccgaatcgt
     5101 tctcccggat gccatgatgg taacgattgt ctcaagcatt aaaggaaatt cagaagcgaa
     5161 tcagattgtg cagaagacag attctcgctc tcaaattatc gcagaggtaa atgaagcgaa
     5221 gccaaaacag tttgaagagg aaaaaactca cgatactcca ttgtccattg aaccagcgga
     5281 acaggcggat caagcagaga aaacggagga gactccggct gcttccccgc catcgggtga
     5341 tcagggacag actgaaacag tggagttgaa tggaggcggg ccgaactcaa ccggagctga
     5401 atttgtctca acgacatcgg aggatgcctt agccaagttc ttacttcagg ttcgtcagac
     5461 cattgaacgt cataaacagt atccccggcg ggctcgcttt cagggaattg agggaaccac
     5521 ccagcttcgc ttccgcattc tgcccaccgg tgagcctacc gagattcagg tggtgcagtc
     5581 ttcacagtct gcaatactgg atgaagaatc ggttgcagcg gtgaagcggg tgacacgatt
     5641 cccgcagcca ccggtaaaat cgccgatggg gattttaatt cgattgccgt tcgtttttca
     5701 cttggaagaa ggagggtaga tgtagcgaga ataaccttat cgttagaatt aaagaaaagt
     5761 tttcttgaga acgggaaccc tgtgtgaatc agggacgggg acgccactgt aagcggggac
     5821 aaatccccaa cagccactgt ctgagtcaag tctttcccca gctcgcaatg cgagcgagaa
     5881 ggggaggctc catccggctt taccgatgga gggggcgacg caagccccta taaaaaggat
     5941 gggaaggcgg ggaggagaaa gatccgcaag tcaggagacc gatcaagaaa gccttacgca
     6001 ctcaaacctt ttcgaaacca aaaggaggca caaaatgcag acaagattgg caactgtggt
     6061 tatattaatg ggaacactga ttggaatgac gaccggactg tgggcagagg aagcgccgga
     6121 agaagcaaca gcggaagcgg tggtagtgac cgctacaaag acagagacgc cgattactga
     6181 ggtggcaagt tcagtttacg taattgacag caaaacgatt gaagacaaaa a
//
LOCUS       DATMKH010000293         1515 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1102028, whole genome shotgun sequence.
ACCESSION   DATMKH010000293 DATMKH010000000
VERSION     DATMKH010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1102028"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /locus_tag="VNV63_03560"
     CDS             complement(<1..773)
                     /locus_tag="VNV63_03560"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HXC61729.1"
                     /translation="MSIPVAVLTSLVLALLAVVSAFFSGMETALFSINGFQVRRWRER
                     EPVVAAQFEKLMLNSRGVLSVILLTDTLVNVPLIIIALDFVNAIPMPVPNWVKTLVIL
                     GLIVFVCDLLPKLLALADPFRFSKSALQILAVWMPVAEPYSRSLQELAEKIADLFAKK
                     RPHPQDHLDEEELETLVELGVEEGELSPGEKEMIQEIIKLGDKTVKDCMTPRVDAFTI
                     SDTLSNEEVIDQVRVKRYGRVPVYGDSPDEILGVLDVKS"
     gene            863..1333
                     /locus_tag="VNV63_03565"
     CDS             863..1333
                     /locus_tag="VNV63_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HXC61730.1"
                     /translation="MKKGEKIQAFVESLGGGLAHDPCYEGYFVCFNDQKYYEAHDVLE
                     HLWLKEQGPSHQFFKGLIQLAGAFVHLQKQYRRPEHPTDGRRLHPAWRLFKLAYANLE
                     PYLPRFLDLDVGSVLELCLKNVVAIEGAEFKTNPWSPVAAPTLALSIQGASGDK"
BASE COUNT          359 a          404 c          397 g          355 t
ORIGIN      
        1 aagctcttca cgtccagaac acctagaatc tcatccgggc tatccccgta aacaggcact
       61 ctcccgtacc gcttcacccg gacctggtcg atcacttcct cgttcgagag cgtatcggaa
      121 atcgtaaaag cgtccacgcg cggtgtcatg cagtctttga cggttttgtc acccagtttg
      181 atgatctcct ggatcatctc cttttccccc gggctgagtt ctccttcttc cacacctagc
      241 tccaccaagg tttccagctc ctcctcatcc aggtggtcct gtgggtgtgg tcgctttttt
      301 gcgaaaaggt ccgcgatttt ttcggcgagc tcctgcagag atcgcgaata tggttctgca
      361 accggcatcc atacggcgag gatttgaagg gcgctctttg aaaatcgaaa cggatccgcc
      421 agcgccagga gctttggcag cagatcgcaa acaaaaacga tgagtcctaa aattacaagg
      481 gttttgaccc aattcggtac cggcatcggg atggcgttca cgaaatcgag agcgataatg
      541 atcagcggaa cgttgaccag cgtgtcagtt aagaggatca cgctcaggac acctctggag
      601 ttcaacatca atttttcaaa ctgggccgcg acaaccggtt cgcgttccct ccagcggcgc
      661 acctgaaatc cgttgatgga gaagagggct gtttccatgc cggaaaaaaa ggcagagacg
      721 acggccagca gagcaagtac taaagacgtc aaaacagcga ctggaatcga catttgcctt
      781 ggtccacctc ggcggatctc cccattgaac ggaattctct ttgcgataag cgggctgaca
      841 agcaaattca aagcaggcgc gaatgaaaaa aggcgaaaag atccaagcat ttgtggagtc
      901 tctgggcggg ggattggccc acgacccttg ttatgagggc tatttcgttt gctttaatga
      961 tcagaaatat tacgaagcgc atgatgtgct ggaacacctc tggctgaagg aacaaggccc
     1021 ctcccatcaa ttttttaaag gactcatcca gctggccggg gctttcgtgc atctgcagaa
     1081 gcagtacagg cgtccggaac atccaaccga cgggcgccga cttcatccgg cttggcgatt
     1141 atttaaactc gcgtacgcca acctcgaacc atacttgccc aggtttctcg atctcgatgt
     1201 cggttcggtc ctcgaacttt gcttaaaaaa cgtcgttgcg atcgagggag cggagttcaa
     1261 aaccaatccc tggagcccgg tcgctgcgcc aactcttgct ctgagtatcc agggagcctc
     1321 tggcgataaa tgagttctcg attgtcttaa cctggaaccg aaatagagtg tgcgcgagtt
     1381 ttgaaataac atgcggagcg ttgtaagaag tctttttgtg atagccgccg ttctctggtt
     1441 cgctgctgga gcggaggcgc agatggcggc agcctatgcg atcgccgatc agaccaccgg
     1501 ttatgttctc gaggc
//
LOCUS       DATMKH010000294         1016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3645939, whole genome shotgun sequence.
ACCESSION   DATMKH010000294 DATMKH010000000
VERSION     DATMKH010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3645939"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            259..>1016
                     /locus_tag="VNV63_03570"
     CDS             259..>1016
                     /locus_tag="VNV63_03570"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXC61731.1"
                     /translation="MSAFSGADSSQELTTDECRRVMDEMEKVNPNLFLILTGGEPFIR
                     KDIFEIASYAADKGFTVVLGTNGVLLREKQAKEMRQSGIQGASISLDSTDAVKHDEFR
                     HLKGAWEGAVRATSVLKEEGLDFSMHMSVMDWNVAEVPAMIELSKTLGAKVLNFFFLV
                     RTGRGENLEDIIPHQYEEILTYLARAQGVGKGNGSGQPTVFDQIEDPWTIPVGQSGDL
                     ILRAKCAPHFRRIIYQLDPNSPLLKNYAQGSCPAG"
BASE COUNT          263 a          273 c          266 g          214 t
ORIGIN      
        1 gatcgagaag cacgccaaaa ctattggggc atccaccatt aacctggaaa tcgcccacca
       61 agggattgaa gaaggcaagc gcacgatgga agacgccatg cgcaatcccg cattgctgga
      121 tgagatcatg gccaagctca aggtcagcaa gggcgaaacc aagaatccat aattctaagc
      181 gatcccgtgg cctatcaagc ctattcaatc tcctggaatc tcacacagcg ctgtaacctg
      241 acctgcaccc attgctacat gagcgccttt tcgggcgccg actcctccca agaattgaca
      301 accgatgaat gtcgccgtgt catggacgag atggaaaagg tcaatcccaa cctttttctc
      361 atcctcacgg gcggcgagcc gttcatacga aaagacatct ttgaaatcgc ttcctacgca
      421 gccgataagg gatttaccgt tgtactggga accaacggcg tgcttctccg ggaaaaacag
      481 gcaaaagaaa tgcggcaaag cggtatacag ggcgcttcga tcagccttga ctccaccgat
      541 gcggtcaaac atgacgaatt tcgtcatctc aaaggcgcct gggaaggagc tgtccgggcg
      601 acttcggttc taaaagaaga gggtctcgat ttttcgatgc atatgagtgt catggactgg
      661 aacgtcgccg aggttccggc gatgatcgaa ttgtcaaaaa cacttggggc caaggtgctc
      721 aatttctttt tcctggttcg gaccgggcga ggcgaaaatc tcgaggacat tatcccgcac
      781 cagtacgagg agatattgac ctacctggcg cgagcgcagg gggtgggaaa gggaaacggc
      841 tcgggacagc cgaccgtttt cgatcagatt gaagatccct ggacaatccc tgtcggtcag
      901 agcggcgatc ttattctccg cgccaaatgc gcccctcatt tccggcgaat catttatcag
      961 cttgatccga actctcctct cttaaagaac tatgcgcagg gaagttgccc tgccgg
//
LOCUS       DATMKH010000295         1104 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1736530, whole genome shotgun sequence.
ACCESSION   DATMKH010000295 DATMKH010000000
VERSION     DATMKH010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1736530"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..990
                     /locus_tag="VNV63_03575"
     CDS             <1..990
                     /locus_tag="VNV63_03575"
                     /inference="COORDINATES: protein motif:HMM:NF020660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase/4-alpha-glucanotransferase
                     domain-containing protein"
                     /protein_id="HXC61732.1"
                     /translation="NLIDSHSGASGVRLTKMDLDRDGYQELLAETAWMNLYLKPSEGA
                     TAIEIDYRPKALCLTHLPMRRPEAYHEKLKTAPVSTAHGSESIASIHERVAVKASAAD
                     SKLIYDRHLRWSFRDCVLNGNITLTEFETSQYQEISGFADQPYETKSALSDAEKAILS
                     FHRTDSVDGLPLTVKKIYTLWASRAELAVNYSFQSVERQGQARGQAPAIDARRDYYWG
                     VELNLTLLAGSDPKRYYHFPGRVVENTPLNSKGILEDLTEARLVDEWQGIEIRLEFKP
                     AVRFWRFPIETISQSEEGMESNYQGSCLVALWPLTLPLKEKHESLITMKIVER"
BASE COUNT          285 a          288 c          294 g          237 t
ORIGIN      
        1 aacctgatcg attctcattc gggcgcatcc ggagtccgcc tgacgaagat ggacctggac
       61 cgggacggat accaggaact gcttgctgaa acagcatgga tgaatctcta cctaaaaccc
      121 agtgaagggg caaccgccat cgagattgat taccggccga aggcgctctg cctgacccat
      181 ctgcccatgc gccggccgga ggcctatcac gaaaaactca agacggcgcc ggtctccact
      241 gcgcacggat cggaaagcat tgcttccatc catgaacgcg ttgcagtgaa agcatcggca
      301 gcagatagca aactcatcta tgatcggcac ctgcgctggt cttttcgtga ctgcgtctta
      361 aacggcaata taacactgac agaatttgag acttctcaat atcaagagat cagtggtttt
      421 gccgatcagc cctatgaaac aaaatccgcg ttgtctgatg cagaaaaagc cattctctca
      481 ttccatcgga cagactccgt agatgggctg cccttgactg ttaaaaaaat ctacacactt
      541 tgggcgtcac gggccgagct cgccgtaaac tacagcttcc agagtgtaga aagacaaggg
      601 caggcgcggg gacaagcccc tgcgatcgac gcacggcgcg actattattg gggtgtcgag
      661 ctgaatctca ccttgctggc cgggtcggat cccaagcggt attaccactt tcccggcaga
      721 gtcgtagaga atacaccgct taacagcaaa ggcattctgg aagatttgac ggaggcccgc
      781 ctggtggatg aatggcaggg gatcgagatc cgtcttgagt tcaagcccgc ggtgcggttc
      841 tggcgatttc cgatcgagac gatctctcaa tcggaggagg ggatggagtc taactatcag
      901 ggttcctgtc ttgtggccct ttggccgttg actcttcctc taaaagaaaa gcacgagagt
      961 ctaatcacga tgaaaatcgt tgagcgttga ttttaaaatg accgcattaa aattcgatgg
     1021 aaacggagga tcataaacga cgatgcctga attaaggaaa gaccctatcc tcggtcgctg
     1081 ggtcatcatc gccaccgaac gcag
//
LOCUS       DATMKH010000296         4544 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4616296, whole genome shotgun sequence.
ACCESSION   DATMKH010000296 DATMKH010000000
VERSION     DATMKH010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4544
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4616296"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /locus_tag="VNV63_03580"
     CDS             <1..570
                     /locus_tag="VNV63_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HXC61733.1"
                     /translation="REKTSEFLEDGKVDQEEMPAYSELKRRLGFEYNGMVLHEYYFGN
                     LKRDGGDDPEKGSTFFKMTESDFDSYKNWKTDFIGVGKMRGVGWAICYHDPATGRLSN
                     HWITLHEIGNIAGFNPILVMDVWEHAFLLDYKPAEREKYIEAFFSNIDWDAIEDRLYN
                     SAALTAVKLQMSGPTSNRHKLSLERGGKI"
     gene            744..1049
                     /locus_tag="VNV63_03585"
     CDS             744..1049
                     /locus_tag="VNV63_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HXC61734.1"
                     /translation="MKIFVGNLSRDAMDDDLRQAFEAFGKVESATVIKDKFTGTSRGF
                     GFVEMSSSSEAQAAITGMNGKDLKGKAMNVNEARPREERGGGGGREDRGGPGGKRRY"
     gene            complement(1082..1927)
                     /locus_tag="VNV63_03590"
     CDS             complement(1082..1927)
                     /locus_tag="VNV63_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HXC61735.1"
                     /translation="MKKWLIVIGVVLVVLIIGGSPFFIVDVTQTAIVVQLGKPVRTVI
                     EPGLQFKIPFIQNVIHFDKRLLDYDTTSQDVITQDKKTLLLDNFAKWRIKDPLKVYEA
                     FQTQQAALGRLDDIIYSELRVELGRHDLVEIVSTARSKIMETVTARANVKAAVYGIEL
                     DDVRIKRADLPEQNEKAVFTRMEAEREREAKRYRSEGAEEAQKIRSEAEKEREIILAE
                     AYKISQTTMGEGDAKAFRIYAQAYQKDVEFFEFMRTMEAYKKALDKETTLVLSPDSEF
                     LKYLK"
     gene            complement(1924..2946)
                     /gene="hflK"
                     /locus_tag="VNV63_03595"
     CDS             complement(1924..2946)
                     /gene="hflK"
                     /locus_tag="VNV63_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020879986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HXC61736.1"
                     /translation="MAWGQRSQEEMPDFERAAREFRERLRKTFGGGGLGGWFPTIGLI
                     LALIAAWQSFYIVAPNEQGVVKRFGRVVRLTPPGPHFKIPLIETVLIPQITQLHRIEI
                     GFRSDAPGGTKTIPSEALMLTGDMNILSVELIVQFRIRDSKDFLFNVADLGETITKAT
                     EAAIRQVIGESLLDEALTTGKAEIQQKTQDLLQKILDDYHAGVQVAAIQLQDVNPPDQ
                     VAGAFKDVASAKEDKEKLINQSQSYRNDIIPKAKGEAAQIVNQAQAYSQARVRRAEGE
                     AARFNQMLKEYQQNKKVIRTRLFLETMEEVLPGIDKVIIDKKFGNQLLPYLPLGRSQK
                     PAEVKP"
     gene            3187..3543
                     /locus_tag="VNV63_03600"
     CDS             3187..3543
                     /locus_tag="VNV63_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017726353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HXC61737.1"
                     /translation="MLTFFGYPKCSTCANAKKNLAKLGREVNEIDITASPPSAKELHR
                     LISKSGRPYTDFLNRSGLQYRALNMKEKVKTLPEDTVIKLLASEGRLIKRPIVTDGTR
                     VTVGFSPEEFSKIWGR"
     gene            3570..4085
                     /locus_tag="VNV63_03605"
     CDS             3570..4085
                     /locus_tag="VNV63_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HXC61738.1"
                     /translation="MNLWLRFIIVLLTAFFRQALGPLDESVLTFRVWPNDLDINGHMN
                     NGRYLTLMDLGRLDVIVRTGMGRTVLRRKWYPLVGSATIRFRFSLEPFQSYQLKSRII
                     CWDKKWFFIEQRFERRGEIIAVGWVKGLLRGPGGNVPSSEVLHSINADIPSPEMPSAI
                     RLWQQVYENKE"
     gene            4138..>4544
                     /locus_tag="VNV63_03610"
     CDS             4138..>4544
                     /locus_tag="VNV63_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61739.1"
                     /translation="MKSVMICFLFGVLISDHVFAQETISPSQQFIQEGDRHWDLRAEG
                     SQGARADSKEIDQALSDYRQAVASDSGSLAVRWRLMRALYFKAEYTTNNNDEKKKIFD
                     EGKAIGEEALQRARQEAAQRTGKPMEKADPAELA"
BASE COUNT         1053 a         1145 c         1273 g         1073 t
ORIGIN      
        1 agagaaaaaa cttcagaatt tttagaagac gggaaagttg atcaagaaga gatgccggcg
       61 tactcggagc tcaaacgtcg tttaggtttt gaatacaacg gcatggtgct gcacgaatat
      121 tattttggta atctaaaaag agacggcgga gacgatcccg aaaagggctc cacttttttc
      181 aaaatgactg aatcggattt cgacagttat aaaaactgga aaacggattt tatcggtgtt
      241 gggaagatgc gcggcgtagg ttgggctatt tgctatcatg atcctgccac cggccgcctc
      301 tcgaatcact ggattacttt gcatgagatc gggaacattg ctggtttcaa tccgatattg
      361 gtaatggatg tatgggagca tgcctttttg ctggattaca aacctgcgga gcgcgaaaaa
      421 tacattgagg catttttctc aaacattgat tgggacgcca tcgaagaccg gctatataat
      481 tcggctgctc ttacggcggt gaaattacaa atgagcggtc caacaagtaa tcgtcataaa
      541 ttaagcctgg agcgaggagg gaaaatatga gagcagcgaa ggcatttaca agagagatta
      601 ccggccgaaa atccttttaa tggattgtat tattgatttc atgatcccta tcagttttat
      661 aaaatcttgc aatcatcggc catccggagt ataaagaaag gccgtcaggc aattaaccgc
      721 acattcagag agaggagaat cgaatgaaaa tttttgtggg taatttatct cgcgatgcga
      781 tggatgatga tttgcgtcag gccttcgagg cattcggaaa agtcgaatcg gccaccgtga
      841 tcaaggacaa gttcacggga acgtcaagag ggttcgggtt tgtggagatg tcctccagct
      901 cggaagccca ggcggcgatt accggcatga acgggaaaga tttaaaaggc aaggcgatga
      961 acgtcaatga ggcacgtcca cgcgaagaac gaggaggcgg gggaggacga gaagatcgag
     1021 ggggacccgg aggcaagcga cgctactgat tatccctatc ccggtgtcgg tccgtagcgg
     1081 tctacttcag atattttaag aattccgaat cgggactcaa aaccaaggtg gtttctttgt
     1141 cgagcgcttt cttgtaggcc tccatcgtac gcatgaactc gaagaattcg acatccttct
     1201 gataagcctg ggcataaatc cggaatgcct tcgcgtcgcc ctctcccatg gtcgtctgcg
     1261 agattttata ggcttcggcc agtatgatct cccgttcttt ctcggcctct gagcggattt
     1321 tctgcgcctc ctccgcgccc tcggagcggt accgcttcgc ctcgcgctcg cgttcggcct
     1381 ccatccgggt gaagacagcc ttctcgttct gctccggaag atcggcgcgc ttgatcctca
     1441 catcatccag ctcgatccca tacaccgccg cctttacatt cgcccgtgcg gtaacggtct
     1501 ccatgatttt ggatcgggcc gtcgagacga tctcgacaag atcatgtcgt cccagttcca
     1561 cccggagttc cgaatagatg atgtcgtcca gccgcccgag cgccgcctgt tgagtctgga
     1621 aagcctcgta caccttgagc gggtctttga tccgccactt ggcaaagttg tcaagcagaa
     1681 gcgtcttttt gtcctgtgtg atcacgtcct gcgaggtcgt gtcgtaatcg agcagccgtt
     1741 tgtcgaaatg gatcacgttc tgaatgaacg ggatcttgaa ttggagcccc ggctcgatca
     1801 ctgtccgaac cggcttgccg agctggacca cgatcgcggt ctgggtcaca tccacgatga
     1861 agaagggcga gccgccgatg atcagaacaa cgagcacgac gccgatgacg atcaaccatt
     1921 tcttcatggt tttacctcgg ccggtttctg tgaacggccc agcgggagat aaggaaggag
     1981 ctgatttccg aatttcttgt cgataataac tttgtcgatg ccgggaagca cctcctccat
     2041 cgtctccaaa aacaggcgtg tccggatcac ctttttattc tgctggtact ccttcagcat
     2101 ctggttgaac cgcgccgcct cgccttcagc ccgccggacg cgcgcctgac tataggcctg
     2161 ggcctggttg acaatctggg cggcctcgcc ttttgccttg gggatgatgt cgtttcggta
     2221 actctgggac tgattgatca acttttcctt gtcctctttc gcgctcgcga cgtctttgaa
     2281 cgccccggcg acttgatccg gtggattgac gtcttgaagt tggattgcgg cgacctgcac
     2341 gccggcgtgg taatcatcca ggatcttctg gagcaggtcc tgtgttttct gctggatctc
     2401 ggccttgccg gtcgtgagcg cttcatcaag aaggctctcg ccaatcacct ggcgaatcgc
     2461 agcctcggtc gccttcgtga tcgtttctcc gaggtcggcg acattgaaga ggaaatcttt
     2521 cgaatcgcga atgcggaact gaacgatcag ctcgaccgaa aggatattca tatcccccgt
     2581 cagcatcagg gcctcgcttg gaatcgtttt cgtgccgccg ggggcatcgg accggaagcc
     2641 gatctcgatg cgatgcagct gggtgatctg cggaatcagg acggtttcga ttagggggat
     2701 cttgaaatgg ggaccgggcg gagtcagccg aacgacgcgg ccgaaccgtt tgaccacacc
     2761 ctgctcattg ggggcgacga tgtagaagga ctgccaggct gcgatgaggg cgaggatcag
     2821 gccgatggtt ggaaaccagc cgccgagtcc tcctccgccg aacgtctttc gcagccgctc
     2881 acggaactcg cgcgcagcac gctcaaagtc gggcatctct tcctgcgatc tctggcccca
     2941 tgccattcga cggtctccta aggaaagtgg agggtagcac ggtcgaactc ctttgtcaac
     3001 gcgatgaaag cttgtctcaa tcgaaccaga ccctgtcggg ataagcagaa gctgcggatg
     3061 aaaattattc caaaaataga cccgtttttg ataaaatcgg atcaatcgct caacaccgaa
     3121 gccaaccgcg ttgcatcggt ggttggtgat ctggtagtat ccacaccaat ttcaggagcc
     3181 attgcaatgc ttactttttt cggctatccc aaatgcagta cttgtgcaaa tgcaaaaaaa
     3241 aatctggcga agctcggtcg cgaggtgaac gagatcgaca tcaccgcctc accgccatcc
     3301 gcgaaagagc tgcacaggct gattagcaag agcggccggc cgtacaccga ttttctgaat
     3361 cgcagcggcc tgcagtatcg cgccctcaac atgaaagaga aggtcaagac actgccggag
     3421 gatacggtca tcaagctgct cgcatcggag ggtcggttga tcaaacggcc gatcgtgacg
     3481 gacggaacgc gggtgaccgt tggattttcg ccggaggagt tttcaaagat ctgggggcgg
     3541 tgatgagaac accggcttag tgttgtgata tgaacctgtg gttgcgcttt atcatcgtgc
     3601 ttctgaccgc ttttttccgg caggccctgg gaccgctgga tgagtcggtg ctcactttcc
     3661 gtgtctggcc gaacgacctg gatatcaacg gtcacatgaa caacggccgg tatctcactc
     3721 tgatggatct gggccgactg gacgtaatcg tccggaccgg tatgggaagg acggttttac
     3781 gccgtaaatg gtatccattg gtgggctccg ctacgattcg gttccgcttc tccttggagc
     3841 cttttcagag ctatcaattg aagagccgta tcatctgctg ggataagaaa tggttcttta
     3901 ttgaacagcg ctttgaacgc cgcggtgaga taatcgcggt tggatgggtg aagggtttgc
     3961 tccgcggccc tggcgggaat gttccttcca gcgaggtgtt gcactccatc aacgcggaca
     4021 tcccttcacc ggaaatgccg tcggcgatcc ggctctggca gcaggtttat gagaataaag
     4081 agtagaacag gaatcgtgct tttcactttg gaatagattt cattgggagg tgaataaatg
     4141 aaatcggtta tgatatgttt tcttttcgga gttcttatct ctgatcacgt cttcgctcag
     4201 gaaaccatat cgccgtcaca gcagtttatt caagagggag accggcattg ggaccttcgt
     4261 gcagaaggtt ctcaaggagc gcgggcggat tcaaaagaga tcgaccaggc gctctccgac
     4321 taccggcaag ctgttgcatc ggactcaggg tcgctggcgg tacgctggcg gctgatgcgg
     4381 gccctctact ttaaagcaga atacacaacg aacaacaacg atgagaagaa aaagattttt
     4441 gacgaaggaa aggcgatcgg ggaagaggcg cttcagcgcg cacggcaaga agccgctcaa
     4501 cgcaccggca agccgatgga aaaggccgat cccgcagagc ttgc
//
LOCUS       DATMKH010000297         1184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1420339, whole genome shotgun sequence.
ACCESSION   DATMKH010000297 DATMKH010000000
VERSION     DATMKH010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1184
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1420339"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..170)
                     /locus_tag="VNV63_03615"
     CDS             complement(<1..170)
                     /locus_tag="VNV63_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXC61740.1"
                     /translation="MTPAGRTGGLPSVRVGVIGVGYLGQHHARIYSQLPGVTLIGVAD
                     TVRERADEVAKAA"
     gene            complement(167..973)
                     /gene="lpxI"
                     /locus_tag="VNV63_03620"
     CDS             complement(167..973)
                     /gene="lpxI"
                     /locus_tag="VNV63_03620"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006419874.1"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HXC61741.1"
                     /translation="MEKIGLIAGNGRFPQIFAENAKRMGLEVIAVAHEGETLPELENY
                     VDKIFWIRVGQLGRLIKIFHNEKVTDAVMAGGIRKTRLFSGARPDLRSLMLLAKVKTR
                     YDDNILRAVAKELEDEGIRMRESTLYLAPILAQKGTMTSRPLSKREQADIQFGWEIAK
                     AIGRREIGQCVVVKDRVLLAVEAIEGTDETIQRAGRLGGEGTVVIKVSKPHQDLRFDV
                     PAVGPQTIESMKAVRAAVLALEAGKTILLDREQMLRQAEEARISVVGVEQ"
     gene            complement(945..>1184)
                     /locus_tag="VNV63_03625"
     CDS             complement(945..>1184)
                     /locus_tag="VNV63_03625"
                     /inference="COORDINATES: protein motif:HMM:NF025097.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein]--UDP-N-
                     acetylglucosamine O-acyltransferase"
                     /protein_id="HXC61742.1"
                     /translation="LYGLNLIGLRRHGFSHDRIAKLKNAYQVLFQEKLPLKEAIKRVR
                     EEIKGSEDVDQLLAFIESSERGVYRKHGEDRPDRG"
BASE COUNT          256 a          388 c          256 g          284 t
ORIGIN      
        1 gcagctttgg ccacttcatc cgcccgctcc cggacagtat cggccacgcc gatgagtgtc
       61 acacccggaa gctgcgaata gatacgggca tggtgttgtc caagatatcc gacaccgatg
      121 accccgaccc gaaccgaagg aagcccgcct gtcctgccgg caggcgtcat tgctctaccc
      181 cgacaacgct gatcctggcc tcttccgctt gcctcaacat ctgctctcta tccaacaaga
      241 tcgtctttcc tgcttccaag gccagcaccg cagcacggac cgctttcatc gactcaatgg
      301 tctggggacc caccgccggg acatcaaagc gaaggtcctg atgaggcttg ctgactttaa
      361 tcacgaccgt accttcacct cccaaacggc ccgcgcgctg gatcgtctcg tctgttcctt
      421 cgatcgcttc taccgcaagc agcactcgat cttttaccac cacgcactgg ccgatctcac
      481 gccggccgat cgccttggca atctcccaac caaactggat atccgcctgc tctcgtttcg
      541 aaagcggcct tgaggtcatc gtcccttttt gcgccagaat gggtgcaaga taaagcgtgg
      601 actcccgcat ccggattcct tcatcctcca gctccttcgc caccgcacgg agaatattat
      661 catcgtatct ggttttgact tttgccaaga gcatcaatga acgcagatcc ggtctggctc
      721 cagagaacag ccgcgtcttc cggatacctc ccgccatcac ggcatcggtt accttttcgt
      781 tatggaagat tttgatgagg cggccgagct gacccacgcg aatccagaaa atcttatcca
      841 catagttctc taattccggc agggtctccc cctcatgggc caccgcaatg acctcaagac
      901 ccattctctt tgcattctcg gcaaagatct gtgggaatcg cccgttaccc gcgatcaggc
      961 cgatcttctc catgtttccg gtacactccc cgttcagaag attcaataaa ggccagtaac
     1021 tgatcgacat cttctgaccc tttgatctcc tctcgaaccc gttttatggc ctctttaagc
     1081 ggcagttttt cctgaaacaa aacctgatat gcattcttta acttggcaat ccgatcgtgt
     1141 gaaaatccat gccgcctcaa tccgatcagg tttaaaccat aaag
//
LOCUS       DATMKH010000298         4100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1420543, whole genome shotgun sequence.
ACCESSION   DATMKH010000298 DATMKH010000000
VERSION     DATMKH010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4100
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1420543"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1179)
                     /locus_tag="VNV63_03630"
     CDS             complement(<1..1179)
                     /locus_tag="VNV63_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HXC61743.1"
                     /translation="MTQQLEQIIQKIKAYNPHAEVELLKRAFEFSAKAHAGQLRHSGE
                     PFLQHPLQVAEIITHLKLDVPSIVAGLLHDTVEDTGATIQEIEQNFGKEVSHLVDGLT
                     KISKIEFKSHEDKQAENFRKMVLSMAEDLRVVLIKLADRLHNMRTLEALPEHKQRRIA
                     QETLDIYAPLANRLGIGWMKYEMEDLCLRYLKPEIYFNLIKKVAKQQEEREKYIQEVI
                     GVVKKSMVEYKCPGLVLGRSKHLYGIYQKMERQGIPFEEVYDLAAIRIITDTKMNCYA
                     ILGMIHSLWRPVPGRFKDYIGVPKSNLYQSLHTTVIGPKGQHVEFQIRTEEMHGVAEE
                     GIAAHWKYKEKGQIDSKDDKLFGWLRQLVEWQTDLMDSRQFMDSVRLDLIQDVVYVFT
                     P"
     gene            complement(1378..2955)
                     /locus_tag="VNV63_03635"
     CDS             complement(1378..2955)
                     /locus_tag="VNV63_03635"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HXC61744.1"
                     /translation="MASEIPLSGRLRDTRLPTLLRFLQRNKKTGILNIHRNDLDKSIY
                     FKNGDIVFATSKYPDDRLGEVLLKMGRITLDQYETSVRLLKQTGKRQGTILVEQGFIA
                     PKDLFMAVTYQVKEIILSLFTWIEGDYLFQEGNLPSEEVITLRMSTARLIYEGVHRNQ
                     DWTRLRRELPPISTQLQITTDPLVLFQDLGLEPPNRQFISLIDGNRTVEELFRQSALP
                     VFETLKCLYFLIAIGIVEAKTVEVKTAEPIPSSEDQTSPSFHPEPASISREQEIQIEV
                     QEEKREDVEESSHELFKEKDETEEFNKQKIREAHAAMKDQDHYEVLGVRRDTPKDEIK
                     KAYFILAKAYHPDRHFQSVMEDVKGQLEELFQRITEAYDTLLMERKRKEYDLTLIMSK
                     YGKKQPAAASEETDEARAVQQFQRGEEAIKRGDYREAVHSFQWAVRLKPKQAQYYTAL
                     GKALAKTPRRIHDAEQNFLKAIELEPSNAEHYIALGFLYKEGKLNQRAIRQFEEALMW
                     DPENKKAKAELQQLKGK"
     gene            complement(2980..3918)
                     /gene="secF"
                     /locus_tag="VNV63_03640"
     CDS             complement(2980..3918)
                     /gene="secF"
                     /locus_tag="VNV63_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HXC61745.1"
                     /translation="MIEILPKTNIDFIGKRKIAFIFSTLLVLSGVIALIQIGRGNANM
                     GIDFAGGTAVQLKFEKPVKMDEARRSLERNGFPEAELQEFTQGNKLLIRVKSSSAKAG
                     SQAATQENTADRIVAVFAQEFPDNKFVVDSSTEIGPAIGKELRQKAVIAIVFSMIGIV
                     IYIWLRFEFRFGIAAAIATFHDVFAVLGIFYLLDKEITLLIVSALLTLAGYSLTDTVV
                     VFDRIRENLRLRRRDSLEAVINSGINQVLGRTIVVSLTVFLVLVGLFFFGGEVIHDFS
                     FALLLGVIIGTYSSIFVASPLLVVWRGTVGRLLKRT"
     gene            complement(3915..>4100)
                     /locus_tag="VNV63_03645"
     CDS             complement(3915..>4100)
                     /locus_tag="VNV63_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HXC61746.1"
                     /translation="DSHVTTLITAFILFLFGTGPIKGFAVSLSLGITINLFTALVGTK
                     VVFDWINSRKKLEHLSI"
BASE COUNT          940 a         1096 c         1015 g         1049 t
ORIGIN      
        1 cggcgtaaaa acatacacca catcttgaat cagatccagc cggaccgagt ccataaactg
       61 acggctgtcc atcaggtccg tctgccactc cacgagctgc cggagccatc caaagagttt
      121 gtcgtctttg gaatcaatct gccctttctc cttgtatttc cagtgagctg caataccttc
      181 ttctgccaca ccatgcattt cctcggttct gatctgaaac tccacatgct gacctttggg
      241 cccgatcacg gtggtgtgaa gcgattgata gagattggat ttgggaacac cgatgtagtc
      301 cttaaaccgc ccgggcaccg gtctccagag agaatggatc atccccagga tggcataaca
      361 gttcatcttg gtatcggtga tgatacgaat cgccgccagg tcgtaaacct cttcgaaggg
      421 aatgccttgc cgttccatct tctgatagat gccgtagagg tgcttggacc gcccgagcac
      481 aagacccgga catttgtact cgaccatgct ctttttgaca accccgatca cctcctggat
      541 gtatttttcg cgctcttcct gctgcttggc cactttctta atgagattaa aatagatctc
      601 gggtttgagg taacgcagac agagatcttc catctcgtac ttcatccagc cgattccgag
      661 ccgattggct aacggggcgt aaatgtccag cgtctcttgc gcaatgcggc gctgtttgtg
      721 ctccggcagc gcctccagtg tccgcatatt gtgcaatcgg tccgccagtt tgatcagcac
      781 cacgcggagg tcttcggcca tggacaacac catcttccgg aaattctcgg cctgcttgtc
      841 ctcatggctc ttgaattcga tcttgctgat cttggtcagc ccgtccacca agtgggacac
      901 ttcctttcca aaattctgct cgatctcttg gattgttgcg cccgtgtctt cgaccgtatc
      961 gtgaagaaga ccggccacga tggagggaac atccagcttg agatgcgtga tgatctcggc
     1021 gacttgtaag ggatgctgaa ggaaaggctc gcccgaatga cggagctggc ctgcatgggc
     1081 tttggccgag aattcaaaag cccgcttgag cagttccacc tcggcatgag ggttgtatgc
     1141 tttaattttc tgtatgatct gttcgagctg ttgggtcatt gcttaacctg cgttattcac
     1201 tcatcctgaa atttttcaag cggagtctgc gacgtgcaac ttgcagtcta caaacctaaa
     1261 attatcatag tctcgacgca attaattgtc aaccggatgg gatgaaaggg gaaagttgtc
     1321 ttgcagtatc cagcgcacca gccgggcggg tacccggcga gcgagagata gaaaaggtta
     1381 tttgcctttg agctgctgca gctcggcctt tgccttttta ttctccgggt cccacatcaa
     1441 tgcctcttca aactgccgga tggcccgctg gtttagtttg ccctctttat acaaaaatcc
     1501 cagggcgatg tagtgctctg cattggacgg ctctaactca atcgccttaa ggaaattttg
     1561 ttcagcgtca tgtattcgac gaggggtttt tgctaaggcc ttgcccagtg cggtgtagta
     1621 ttgggcctgc ttgggtttca ggcgaacggc ccattgaaaa gaatgaaccg cttctcgata
     1681 atcgccgcgc tttatggcct cctccccgcg ctgaaactgc tgcaccgcac gggcttcatc
     1741 cgtttcctcc gatgccgcag caggctgctt ctttccgtac ttggacatga tcagcgtgag
     1801 atcatactct ttgcgttttc gttccatgag cagtgtatcg taggcttcgg tgatgcgttg
     1861 gaagagttcc tcgagctgac ccttaacgtc ttccattacg gattgaaaat gccgatccgg
     1921 atgatatgcc ttggccaata taaaataggc ctttttgatt tcatccttgg gggtgtcacg
     1981 ccgaactccc aggacctcat aatgatcctg gtccttcatc gcggcatgtg cttcgcgtat
     2041 cttctgctta ttgaattcct cggtttcgtc tttttcttta aacagctcat ggctactctc
     2101 ctccacatct tcgcgcttct cttcttgcac ctcgatctga atctcttgtt cacgactgat
     2161 cgaggcaggc tccggatgaa aagaaggcga cgtttgatcc tccgaagaag gaatcggctc
     2221 cgccgttttg acttcaaccg tcttggcctc tacgattccg atggctatca ggaaataaag
     2281 gcattttaat gtctcaaaga ccggaagcgc agattgtcta aagagctctt ccaccgtcct
     2341 gtttccgtca atcaaggaaa taaattgccg gttcggaggc tcgagcccca ggtcctgaaa
     2401 taagaccagc gggtcggttg tgatctggag ctgcgtactt atggggggca gttctcgacg
     2461 cagtcgggtc cagtcctggt tccggtgaac accctcgtag atcagtcgag ccgtactcat
     2521 acggagcgtg atcacttctt cgctcgggag attcccttcc tgaaagagat aatctccctc
     2581 aatccaggta aaaagactca ggatgatctc tttgacctgg taagtcacag ccataaacag
     2641 gtccttgggg gcgataaaac cctgttctac caggatcgtc ccctgacgtt ttccagtctg
     2701 cttcagcagc ctgacagagg tctcatactg gtccagtgtg atcctgccca tcttgagcag
     2761 cacttctcct aaccggtcat ccggatattt tgaagtggcg aatacaatgt cgccgttctt
     2821 gaagtaaatg gatttgtcca gatcgtttcg atggatattg aggatgccgg ttttcttgtt
     2881 gcgttggagg aaacgcagga gagttggtaa ccttgtatcc cgcagtctgc ccgaaagagg
     2941 gatctcagat gccattgcgg cctttctccg tttaggaatt tatgtccgct ttaagagtct
     3001 cccgacggtc cctctccaga caaccaggag cgggctcgcc acaaagatcg aggaataggt
     3061 cccgatgatt accccgagga gaagggcaaa tgaaaaatca tgaatcacct cgccgccgaa
     3121 aaagaaaagg ccgactaata ccaggaacac ggtcagagac actacaatgg tacggcccag
     3181 aacttgattg atcccgctgt tgatgacagc ctccagcgaa tcccgccgtc ggagcctgag
     3241 gttctcacgg attcgatcga agaccaccac cgtatcggtc aaggaatagc ccgccagagt
     3301 cagcagggcg ctcacgatca gcagtgtgat ctccttgtcc aggagataga agatccccaa
     3361 gacggcgaaa acgtcatgaa atgtcgcaat tgccgccgca atgccgaacc gaaactcaaa
     3421 ccttaaccag atataaataa caataccaat catcgagaag acgatggcaa tgaccgcttt
     3481 ttgtctcaac tccttaccga tagcggggcc gatctcggtg ctcgagtcta cgacaaattt
     3541 attgtccgga aactcctggg cgaagacagc cacgatgcgg tcggccgtat tctcttgagt
     3601 cgcagcctgt gacccggctt tggcggagga ggacttcacc cgaatgagaa gcttgttgcc
     3661 ctgagtaaac tcctgcagtt cggcctcagg gaatccgttc cgctccaggg aacgacgggc
     3721 ctcgtccatc ttgacgggct tttcaaactt caactggaca gcggtgccgc cggcaaaatc
     3781 aatccccata ttggcgttgc ctcgaccgat ctggatcaat gcgataaccc cgctgagaac
     3841 gagcagggtg gagaagataa aagcgatctt ccgcttcccg ataaaatcaa tattcgtttt
     3901 tggtaggatc tcgatcatat gctcaaatgc tccaattttt tccgactgtt gatccagtca
     3961 aataccacct tcgtacccac cagtgccgtg aagagattaa tcgtgattcc caaactcagt
     4021 gagaccgcaa atcctttaat ggggccggtg ccaaacaaga acaggatgaa ggccgtgatc
     4081 aaggtggtaa cgtgtgaatc
//
LOCUS       DATMKH010000299         1241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4309835, whole genome shotgun sequence.
ACCESSION   DATMKH010000299 DATMKH010000000
VERSION     DATMKH010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4309835"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="VNV63_03650"
     CDS             complement(<1..467)
                     /locus_tag="VNV63_03650"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXC61747.1"
                     /translation="MSDIAIRMEQVYKKFRRGETYNSLRDLLPALTGRMFQQQELSAG
                     DKREFWAVQDLSFEVKQGEAFGIIGHNGAGKSTALKMLSRVMKPTKGRMVVKGRLSAL
                     IEVSAGFHQDLTGRENIFLNGTILGMTKREIQSKLDQIIAFSGLEEFIDTPVKR"
     gene            complement(480..>1241)
                     /locus_tag="VNV63_03655"
     CDS             complement(480..>1241)
                     /locus_tag="VNV63_03655"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXC61748.1"
                     /translation="FRYRELLYMIAYRDIRVRYKQSIMGFMWAILMPVLIVLSGVVVR
                     YAYAMASHAPLKTVDIASVAVKSLPWAFLVSSIRFACLSLINNKDLVTKIYFPKEIFP
                     IAAVLASLFDFVVASGALIILLLVLRIGWSPYLAWTPALLLTMVLLAAGIGMIVSAAS
                     LYFRDVKFIVEVFLTFGIFFTPVFYDASMFGDKGKWLLLNPMSPILDGFGAAIARHQS
                     PDLWWFAYSLVCTLLIFLGGYVFFKHLEPGFAESI"
BASE COUNT          330 a          328 c          317 g          266 t
ORIGIN      
        1 cgttttacag gagtgtcgat aaactcttcg agcccggaga aggcgatgat ctggtcgagt
       61 ttcgattgga tttcgcgctt ggtcatgccc aggatcgtgc cattgagaaa gatattctcc
      121 cgtccggtga ggtcctggtg aaagccggca gatacttcaa tcagtgccga aaggcgtccc
      181 ttgacgacca tgcggccctt agtgggcttc atgacccggc taagcatctt taacgcagtg
      241 cttttccccg cgccattgtg gccgatgata ccgaaggcct ctccctgctt tacctcaaac
      301 gagagatcct gaactgccca gaattccctt ttgtctcctg cactgagttc ctgctgctga
      361 aacattcgtc ccgtcagagc ggggagcagg tcgcgcaggg agttgtaggt ctcgccccta
      421 cggaacttct tgtagacctg ctccatccgg atggcgatgt cagacattcg ttttatcctt
      481 caaatgcttt ccgcgaatcc tggctccaaa tgcttgaaga aaacataccc acccaagaat
      541 atcagcaggg tacaaaccag gctgtaggca aaccaccaca gatctgggga ttgatggcgg
      601 gcgatggctg cgccgaaacc atccagaatg gggctcatcg gattcagcaa tagccatttg
      661 cctttgtcac cgaacatgct ggcatcgtag aaaaccggtg taaagaagat tccgaatgtg
      721 agaaatactt cgacaataaa tttcacatcg cggaaataga ggctggcagc ggacacaatc
      781 attccgattc ccgctgccag tagcaccatc gtgagaagaa gtgccggggt ccacgcgaga
      841 tagggactcc aaccaatgcg gagcaccaga agaaggatga tcagggcacc cgaggccacc
      901 acgaaatcaa ataaactggc caacacagcg gcgatgggaa aaatctcctt gggaaaatag
      961 atcttagtca ccaagtcttt attgttgata aggcttaggc aagcgaaccg gatgctggaa
     1021 acgagaaacg cccagggaag agatttcacc gccacactcg cgatatccac cgtctttagc
     1081 ggtgcatgcg atgccatggc atatgcgtat cggaccacga caccggataa aacaatcaaa
     1141 actggcataa gaatggccca catgaagccc atgatcgact gcttgtaccg caccctgata
     1201 tcgcgatagg cgatcatgta gagtagttcg cgatacctaa a
//
LOCUS       DATMKH010000300         1058 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1421465, whole genome shotgun sequence.
ACCESSION   DATMKH010000300 DATMKH010000000
VERSION     DATMKH010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1421465"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VNV63_03660"
     CDS             <1..189
                     /locus_tag="VNV63_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61749.1"
                     /translation="REMGEAGRRRVEKKFTIDAMMDQVTRVYADLLRGTNDRDEGSTQ
                     NGKLQQSQLSNADQGNRK"
     gene            186..>1058
                     /locus_tag="VNV63_03665"
     CDS             186..>1058
                     /locus_tag="VNV63_03665"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXC61750.1"
                     /translation="MSSVPRRISVFMMDLWATVPYYTAYLSKALSKEPVDLTVGSISY
                     YLDPTCFTGRGIKVDPGLLDVVGKYRLPKLPRRILKMIEAKMNLSALTVRFLFSRPDL
                     IHVQYLPMLKWRLPLDFWFLEFCRWRGSKIVLTVHDLLPHDTGDAHRQTFNRLYGTVD
                     GLICHSDHVKTRLGAEFDVPEGKISVIPHGPFFYDLPSTGLQQNSQSLVNEPCDALVL
                     WQGIIFPYKGIDLLLSAWKQVEAKTENVCLVIAGTGAPEFLEQIRGQVKQLDLKRVRL
                     DFRFVSSEELVTLYR"
BASE COUNT          244 a          245 c          307 g          262 t
ORIGIN      
        1 cgggagatgg gagaggccgg cagaagacgg gtggagaaga agttcacaat cgacgcgatg
       61 atggatcaag tcacgagagt gtacgcggat ctgctcagag gcaccaatga tcgtgatgaa
      121 ggctcgactc agaacggaaa gctgcaacaa tcccagttat ccaatgcgga tcaaggaaac
      181 aggaaatgag ctcagtcccc cggcgaatca gtgtcttcat gatggatctc tgggcgaccg
      241 tgccttacta cactgcttat ctttcaaagg ctctgtcgaa ggaaccggtt gatttgaccg
      301 tcgggtcgat ctcttattac ctcgatccga cttgcttcac tggccgcgga atcaaggttg
      361 accccggact gcttgacgtt gtgggaaagt atcgtctgcc gaaacttccg cgcagaatcc
      421 tgaagatgat cgaagcgaaa atgaacttgt ctgcgctgac ggtgcgcttt ctgttttcgc
      481 gtccggatct gatccatgtt caatatctgc cgatgctcaa gtggcgactt ccgctcgact
      541 tttggttttt ggagttttgc aggtggcgtg gttcaaagat cgtgctgaca gttcacgatc
      601 tgcttcctca tgatacgggc gatgcgcaca gacagacatt caatcgactg tacgggacgg
      661 tcgatgggct gatctgtcat tcggatcatg taaagacaag gctgggcgcg gagttcgacg
      721 tgcctgaggg aaaaatatcc gtgatcccgc atggaccgtt tttttatgat ctcccctcga
      781 ccggtttgca gcagaactcg cagagcttag tgaatgagcc atgtgatgcg ctggttttat
      841 ggcagggaat catctttccg tacaaaggaa ttgatcttct tctcagtgca tggaagcagg
      901 tggaggcgaa gactgaaaat gtctgtcttg tgattgcggg cactggcgcg ccggagtttc
      961 tggagcagat tcgcgggcaa gtgaagcagc tcgacctgaa gcgagttcga ctggacttcc
     1021 gttttgtgtc ctcagaagaa ctggtgacac tgtatcga
//
LOCUS       DATMKH010000301         1302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3974640, whole genome shotgun sequence.
ACCESSION   DATMKH010000301 DATMKH010000000
VERSION     DATMKH010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3974640"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="VNV63_03670"
     CDS             <1..221
                     /locus_tag="VNV63_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61751.1"
                     /translation="VDGFLLVVAAHKTPQKLLEEALNVMDPAKAIGLVFNGDDRPVFG
                     HSQYYYSYIHQSRAAKKSSSSGQLSSQP"
     gene            385..>1302
                     /locus_tag="VNV63_03675"
     CDS             385..>1302
                     /locus_tag="VNV63_03675"
                     /inference="COORDINATES: protein motif:HMM:NF025104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HXC61752.1"
                     /translation="MVVFYYADLYDLRIVPNFSRYFSRLPKSLFFVLFPLIVFYTLSP
                     MAEITPNPFISGFLTLLVITGFLLPLRAIFYAILGNKRFIKRALILGASPFAYQLIRE
                     IEAHPRSRYSTIGIVDDIVPSDNLPSRYPFLGSFKDLIKIIDETRPDRIIVALARSED
                     LPLRDLLQTRLHGVVVEDGVKVYERLTGKLAIDSLTPNSLIFSQDFNKRRLEIGLRRT
                     FSLVLALPGLLLIFPLMVIIALAIKLDSEGPVFFVQERVGLRGRIFRLLKFRTMYPAQ
                     QQTSEWVRDNGHRITQIGKRLRKFRLDELP"
BASE COUNT          265 a          351 c          319 g          367 t
ORIGIN      
        1 gggtggatgg ctttctcctg gttgtggcgg cccacaagac gccgcagaag ctcttagagg
       61 aagccttgaa cgtcatggac ccggcgaagg ccatcggtct cgtttttaac ggggatgacc
      121 gtcccgtctt cggacattcc cagtattact atagttacat acatcaatcc cgtgccgcga
      181 aaaaaagctc gtccagcggc cagctttctt ctcaaccgtg acaaggttta gcaaacaatg
      241 acgctcatcc tgcctcttct tgagggaatc ttaatctctg caatcgtgtc cctgatagca
      301 tggacccagg agtataccct atggatccac tggggcgatg ctgcgactgc agtgggacgg
      361 ggggggattc tttccctttg ctgcatggtt gttttctatt atgccgatct ctacgatctt
      421 cggattgtgc cgaacttttc cagatatttc tcccgactac ccaagagctt gtttttcgtc
      481 ttatttccat taattgtttt ttacactctt tccccgatgg cagagatcac cccaaatcct
      541 tttatctcag gctttctgac tctcttggtc atcacaggat ttctcctgcc gttgcgggcg
      601 atcttctacg ccattctggg caacaagcgt tttatcaagc gtgccttgat cctgggagcc
      661 agcccattcg cataccagct cattcgagag atcgaggccc acccccgatc cagatatagc
      721 accatcggta tcgttgatga cattgtccct tcagataatc ttccttcgcg ttatcctttc
      781 cttggatctt tcaaggatct gatcaaaatt atcgatgaga cccgtcctga ccggatcatc
      841 gttgcgctgg ctcggagcga ggatttacca ctccgtgatc ttttgcaaac ccggttgcac
      901 ggggtcgttg tcgaggacgg cgtgaaggta tatgagcgtc ttacggggaa gctggctatt
      961 gattcactta cgccgaacag cctcatcttt tctcaggact ttaacaagcg gaggttggaa
     1021 ataggcctgc gcagaacctt cagtctcgtc ttggcgcttc ccggacttct gctgatcttt
     1081 cccctgatgg tcatcattgc cctggcgatt aagcttgatt cagaggggcc cgttttcttt
     1141 gttcaggagc gtgtcggtct caggggacgg atttttcgtc tcctgaagtt tcgtaccatg
     1201 tatccggccc aacaacaaac gtcggagtgg gtccgggaca acggtcatcg gatcacacag
     1261 atcggaaagc ggctccgaaa atttcgactg gatgagctgc ct
//
LOCUS       DATMKH010000302         2343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4618335, whole genome shotgun sequence.
ACCESSION   DATMKH010000302 DATMKH010000000
VERSION     DATMKH010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4618335"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="VNV63_03680"
     CDS             complement(<1..663)
                     /locus_tag="VNV63_03680"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HXC61753.1"
                     /translation="MKSLLRVVRYIKPHWPLALMTFGAAVLVTLIELLPPWLVKLIID
                     DVIKGGRISWLPWLILGLLSAYFFKNLLAVGRIWSNNFLEQNVIYDLRDQVYRALQRL
                     SISYFENRSTGELMSRLINDVSHVERIFIDGIESILMSGLTLVGVAAVLFYLNWRLAL
                     LSLIPVPFLILGAGFFTRRIHGLYHLIRQKSAQLNALLQDSLSGIRETMSFNRAAYEI
                     DRF"
     gene            complement(660..1199)
                     /gene="def"
                     /locus_tag="VNV63_03685"
     CDS             complement(660..1199)
                     /gene="def"
                     /locus_tag="VNV63_03685"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HXC61754.1"
                     /translation="MSILKVSKLGNPILRKVAAPVPPAEIKRPEFQKFIDDMIETMHE
                     YDGVGLAAPQVHESKLVAVIEVQGNRRYPEAPEIPLLVLINPVFLDQAKEKNMGWEGC
                     LSIEGFRGQVPRATQVKIKALDRNGKEFILEANGFFAVVIQHELDHLMGKVFLDRMSD
                     FSTLTHLKEYERYWLKEEA"
     gene            1309..2127
                     /locus_tag="VNV63_03690"
     CDS             1309..2127
                     /locus_tag="VNV63_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HXC61755.1"
                     /translation="MNTKSVEELKQLAKGFRREIIRITGLAGSGHPSSSFSALEILTA
                     LYFHVLRHDPKNPHWPDRDRFILSKGHAAPGLYTVLANSGYFPVEQLKTLRKFESPLQ
                     GHPEKDRVPGVEASTGSLGQGISIGIGMALAARMDKKDYRVYVVTGDGELDEGQIWEA
                     AMFATHYQLDNLTVIVDRNKQQQDSWCKEVMNTEPLLEKWQSFGWYVIDVNGHDLEQV
                     IAAFAEAKKVKGKPTVIIARTVKGKGVSFMENNTSFHGVAPTPEQVELALKELA"
     gene            2127..>2343
                     /locus_tag="VNV63_03695"
     CDS             2127..>2343
                     /locus_tag="VNV63_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HXC61756.1"
                     /translation="MAPEKKMGLPTRDAYGQALVEIGKNNPNIVVLDGDVSGSTKSRA
                     FAKEFPERFFNFGIAEANMVSAAGGFAS"
BASE COUNT          569 a          626 c          649 g          499 t
ORIGIN      
        1 gaaccgatcg atttcgtagg cggcccggtt aaacgacatc gtctcccgta tgccggagag
       61 agaatcctgt aaaagggcgt tgagctgcgc cgatttctga cgtataagat ggtagagacc
      121 gtggatccgc cgtgtgaaaa agccggcacc caggatcagg aagggcaccg ggatcagaga
      181 gaggagggcc agccgccaat ttaagtaaaa gagaaccgcg gcgaccccga ccagcgtgag
      241 gcccgacatc agaatcgact cgataccgtc aatgaagatc cgttcgacgt gactgacgtc
      301 attgatcagc cgggacatca gctcgcccgt ggagcggttt tcaaagtaac tgatcgaaag
      361 gcgttggagt gcgcggtata cctgatcgcg cagatcatag atgacgttct gctccaggaa
      421 attgttcgac cagatccgtc cgacggcaag gaggttttta aaaaaatagg cggagagcaa
      481 cccgagaatc agccagggca accaagagat ccggccgccc ttgatcacgt catcaatgat
      541 tagcttgacc agccatggcg ggagcaactc aattaaggtc accagaaccg ccgccccaaa
      601 cgtcatcagc gccagcggcc aatgcggttt aatatagcgg acgaccctca gcaaggattt
      661 catgcttctt ccttcagcca gtagcgctca tactctttca gatgcgtcaa ggtggaaaag
      721 tccgacatcc ggtctaaaaa cactttgccc atcaggtgat ccagctcgtg ctgaataacc
      781 accgcgaaaa atccgttcgc ttccagtatg aattcctttc cgttccgatc gagcgccttg
      841 atcttgacct gggttgcacg cgggacctga cctcgaaacc cttctatgct taggcatcct
      901 tcccatccca tgtttttctc tttggcctga tctaaaaaga ctggattgat caaaaccagc
      961 agcgggatct cgggcgcttc gggatagcgc cggttgccct gaacctcgat cacggccaca
     1021 agcttggact catgtacctg cggtgcggcc aggccgaccc cgtcatactc atgcattgtc
     1081 tcgatcatgt cgtcaatgaa tttttgaaac tcagggcgct ttatctcggc cggaggaacc
     1141 ggcgccgcga ccttccgcaa gatcggatta cctaattttg agaccttcaa aatcgacata
     1201 gtgaatgtat atcatatccg ttgctctaaa gcaattggac ctcaaaactc tgttgactgc
     1261 ctggagaggt ttctgctaaa ctgtttttcc aaatttggaa agcctccttt gaacacaaaa
     1321 agcgttgaag aattaaaaca gctcgccaag ggtttccgtc gagaaatcat ccgaatcacg
     1381 ggcctggcag gctccggtca tccgtccagt tctttctcgg ccctggagat tttaaccgca
     1441 ctctatttcc atgtcctccg gcatgatccg aagaatcccc actggccgga tcgggaccgg
     1501 ttcatactga gcaaaggcca cgcggcgccg ggtctctaca cggtcctggc caactccggt
     1561 tattttccgg ttgagcaact taaaacgctg cgcaaattcg agagccctct ccaggggcat
     1621 ccggaaaaag accgcgtgcc gggtgtggag gcctcgaccg ggtcgctggg tcaggggatc
     1681 tcgatcggga tcggtatggc gctcgcggcg cgcatggaca agaaagatta tcgcgtctat
     1741 gtcgtgaccg gtgacggtga actggatgag ggacaaatct gggaagcggc gatgttcgcc
     1801 acgcattatc aactggacaa tctcaccgtc attgtggacc gcaacaagca gcagcaggac
     1861 agctggtgta aagaggtgat gaacaccgag ccgcttcttg agaagtggca gtccttcggc
     1921 tggtatgtga ttgacgtcaa cggccatgac ctggaacagg taatcgcggc cttcgcggag
     1981 gcgaagaagg tgaagggcaa gccgaccgtc attattgcgc ggacggtcaa gggcaaaggc
     2041 gtctcgttca tggaaaacaa caccagcttc cacggtgtgg cgccgacacc ggagcaggtg
     2101 gaactggctt taaaggagtt ggcctgatgg cgccggagaa aaaaatgggg cttccgacga
     2161 gggatgccta cgggcaagcc ctggtggaga tcgggaaaaa taaccccaat atcgttgtcc
     2221 tggatggaga tgtgtccggc tctacaaaga gccgggcctt tgcaaaagaa tttccggagc
     2281 gcttcttcaa cttcggtatt gccgaagcga acatggtttc cgcggccggc gggtttgctt
     2341 cct
//
LOCUS       DATMKH010000303         1378 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4618622, whole genome shotgun sequence.
ACCESSION   DATMKH010000303 DATMKH010000000
VERSION     DATMKH010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4618622"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(425..>1378)
                     /locus_tag="VNV63_03700"
     CDS             complement(425..>1378)
                     /locus_tag="VNV63_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014002225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HXC61757.1"
                     /translation="ADAENPIAMPPLEIGEPTLTMEFMVNASPLAGTEGKFVTSRHLR
                     ERLHRELRTNMALRLEETGTGDLFKVSGRGELHLAILIENMRREGFELAVSKPRVILK
                     QVEGKTLEPYESLIVEAEEAYQGIVIEKLGKRKAEVIKHQVGADGRVRLEYRIPARGL
                     IGFQTEFLTDTKGTGTLHHVFDGYGPHKGEIPTRTRGVLIAMDAGETAGYGLFNLEER
                     GELFVGPGTKVYAGMIVGLHSRDNDLVVNPMKSKKLTNMRAAGSDENITLTPPRLLTL
                     EQAIEFIADDELVEVTPQSIRLRKRVLDENERKRVSRKTNG"
BASE COUNT          282 a          397 c          354 g          345 t
ORIGIN      
        1 gaaattttaa cgaataccgg attcttcgtc aagccaaggt tcccggctct ctccttaaca
       61 agtttcagct attcaaacag atgtagcata ggtcgaggat cgtggtatct tttttcccgg
      121 tttcttgggg gaagtaccca gagcgtgcag ccgattgaaa atcacgacgt tgtcaacaat
      181 atccccgcag ttgaggcagc gggcgataga ggttcccccc tctttttcag taaacatcca
      241 ttcttcaacc atggttccct gacatcgcag gcatctcatt gtatcctcct aagttcgggt
      301 aactgattat cttgggcgtt agtctactcg aacaaagtta ggagaaaatt aaccgctgat
      361 taaaaacttc taatggatag ggatataaac tgtgcctggt ggttttagag gagaaagatg
      421 cttgtcagcc gtttgtcttt ctggagaccc gcttgcgctc attctcgtcg agaacccgct
      481 tccgcagccg gatggattgc ggtgtcacct caacgagctc atcatccgcg atgaactcga
      541 tcgcctgctc caaggtcagc agccgcggcg gggtaagggt gatgttttca tcgcttccgg
      601 ccgcccgcat gttagtgagt ttcttcgact tcatcggatt caccacaaga tcgttgtcgc
      661 gtgaatgcag cccgacgatc attcctgcgt agaccttggt tccggggccc acgaacaact
      721 ccccgcgttc ctcaaggttg aagaggccat agcccgcagt ctctcctgcg tccattgcga
      781 tcaggacgcc gcgtgtcctt gtcgggatct cccccttgtg cggtccatag ccgtcaaaaa
      841 catgatggag cgtgccggtg ccttttgtat cggtcaagaa ttcggtctgg aacccaataa
      901 gacctctcgc cggaatgcga tactccagcc ggacccggcc atcggcaccg acctggtgtt
      961 tgatcacctc cgccttccgt ttacccagct tctcgatcac aatgccctgg taggcctcct
     1021 cggcttcgac gatcagggat tcatagggct cgagcgtctt gccctcgacc tgtttcagga
     1081 ttacgcgcgg tttcgaaacg gccaattcaa atccttccct ccgcatgttt tcgatcagga
     1141 tggccagatg gagctcaccg cggccggaga ctttgaagag atcgccggtt ccggtctcct
     1201 caagacgcag cgccatgttg gtccgcaact cccgatgcag gcgctcgcga agatgccggc
     1261 tcgtaacgaa ctttccttcg gtgcctgcca gcggcgatgc gtttaccata aattccatcg
     1321 tgagggtcgg ctcgccgatc tcgagagggg gcatggctat cggattctcc gcatccgc
//
LOCUS       DATMKH010000304         1102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3025359, whole genome shotgun sequence.
ACCESSION   DATMKH010000304 DATMKH010000000
VERSION     DATMKH010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3025359"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1102
                     /gene="bamA"
                     /locus_tag="VNV63_03705"
     CDS             <1..>1102
                     /gene="bamA"
                     /locus_tag="VNV63_03705"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HXC61758.1"
                     /translation="KIGGIEVLGAEAFSGKKVADQMKENKNGDKYKPELLNDDMKAIE
                     DFYHDEGYLKAVVLSHEEKLNEEMKKVYLTIRVKEGLKYTLGDVNLNGNILFDDKELL
                     KSLGIKKGDLLRKKDLDEGTRKMRSMYADKGYIYAKLTPTMNYDDDLKKADITFEVTE
                     GQVAYVQDIKIVGNYKTQDYVIRRELAVAAGDKFEASKIKLSVQNLYNLGFFDEVNPD
                     VEPGDGPGKEILVFRVKERRTGSISVGGGYSSVDGFVGNVKLEEANLFGKGQHASLDV
                     EFGALRSSFSVGFTEPWLFNTPTSLGINLFDTTRIFTTSGLNPDGTNTFYTETQVGGS
                     ISLGRRLSRYWSIFGSYSLQNVDIFDVDSFYTT"
BASE COUNT          296 a          267 c          316 g          223 t
ORIGIN      
        1 aagatcggcg ggatcgaagt cctgggggcc gaggccttca gcggcaagaa ggtggccgac
       61 cagatgaagg aaaacaagaa cggggacaag tacaaacccg agctcttaaa cgatgacatg
      121 aaggccatcg aggactttta ccacgatgag ggttacctga aggcggtcgt attgagccat
      181 gaggaaaaat tgaacgagga aatgaaaaag gtttatctca ccatccgggt gaaagagggg
      241 cttaaataca ccctagggga cgttaatctc aatggaaaca tcttgtttga cgataaagag
      301 ctcctcaaga gcctggggat caaaaaaggg gacctattac gcaagaaaga cttggatgag
      361 ggcacccgta aaatgcgctc gatgtatgcc gataagggct atatctacgc caagctcact
      421 cccaccatga attatgacga cgatttgaag aaggccgaca tcaccttcga agtgaccgaa
      481 gggcaagtgg cttacgtcca ggacatcaaa atcgtcggaa attacaaaac ccaggattac
      541 gtcatccgca gggagctggc ggtggcggcg ggcgacaagt tcgaggcctc caaaatcaag
      601 ctctcggtcc agaaccttta taacctcggg tttttcgacg aagtcaatcc cgatgtcgaa
      661 ccgggggacg ggccgggcaa ggaaatcctg gttttccgcg tgaaggaacg caggacgggt
      721 tcgatcagcg tgggcggggg atacagctcg gtggacggtt ttgtgggcaa tgtcaaactg
      781 gaggaggcga acctcttcgg caagggccag catgccagtc tggatgtgga attcggcgca
      841 ttgcgaagca gctttagcgt tgggttcacc gaaccctggc tcttcaatac cccgacctcg
      901 ttggggatca atctgttcga tacgacccgc attttcacaa cctccggctt aaacccggat
      961 gggaccaaca ccttttacac cgaaacccag gtcggcggtt ccatcagctt gggaaggcgt
     1021 ttgagccgct attggagcat tttcggcagc tattccctgc agaacgtgga tattttcgac
     1081 gtggactcct tttacacgac ca
//
LOCUS       DATMKH010000305         1982 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2064731, whole genome shotgun sequence.
ACCESSION   DATMKH010000305 DATMKH010000000
VERSION     DATMKH010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2064731"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1159
                     /gene="kaiC"
                     /locus_tag="VNV63_03710"
     CDS             <1..1159
                     /gene="kaiC"
                     /locus_tag="VNV63_03710"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF006799.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="circadian clock protein KaiC"
                     /protein_id="HXC61759.1"
                     /translation="DCMITLDHRVTDQVSTRRMRVVKYRGSPHGTNEYPFLIDESGLT
                     VIPITSMGLNHEASTERVSSGIPKLDEMLEGDGFFRGTSILVSGTAGSGKSSVAAAFA
                     DGACRRGERCLYFAFEESPSQIVRNMKSIGINLQPWLEKGLLEIDAARPSHYGLETHL
                     AGIQKRILEVKPHAVIVDPITNFTTGNTAIDVKAMVSRLIDFLKSQQITTMFVSLTGG
                     GEMIESTEVGISSLMDAWIILRDMEVNGERNRVLYLVKARGVAHSNQVREFVLTSKGI
                     QLVDAYLGGNTVLTGSARLAQKAQERLMDLEHKQEAEATQRRIERERQVLETQIATLQ
                     VEYEAKKDELKRAMGKEQLKETMTITDQATMEQSRKVSVRNSRPNKKYAKR"
     gene            1164..1472
                     /locus_tag="VNV63_03715"
     CDS             1164..1472
                     /locus_tag="VNV63_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012025985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0048511 - rhythmic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock KaiB family protein"
                     /protein_id="HXC61760.1"
                     /translation="MRPARKKDASPVYLKLYIAGQTPKSVIALANLKKICDEHLAGKF
                     NIEVIDLLKNPSLAQGDQILAIPTLIRKLPQPIRKIIGNLSNTERVLIGLDLESRVTI
                     "
     gene            1469..1852
                     /locus_tag="VNV63_03720"
     CDS             1469..1852
                     /locus_tag="VNV63_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock KaiB family protein"
                     /protein_id="HXC61761.1"
                     /translation="MNHQKVIRHLADKIARKTNAKRPKYTLSLYITGTTPQSTRAILN
                     LKRICDQYLKGHYILKIIDIYQQPHLAKDAQIIAAPTLVKKLPIPLRRFIGDMSNEEH
                     LLIGLDLQPRDENGSRAKKGGLKNE"
BASE COUNT          561 a          497 c          519 g          405 t
ORIGIN      
        1 cgattgtatg atcacactcg atcatcgcgt cacagaccag gtttcgacac ggcgtatgcg
       61 tgtggtaaag taccgaggtt ctccgcacgg cacgaatgaa tatccattcc tgattgatga
      121 gtcgggtttg accgtcatac ccatcacgtc catggggcta aaccatgagg cttcgaccga
      181 gcgagtttca tccggaatcc caaaactcga tgagatgctc gaaggcgatg gttttttccg
      241 cggaaccagc atattggttt ccggcacggc cggaagcggg aagagcagcg tggcggcggc
      301 gtttgccgat ggcgcctgcc ggcgcggcga acgatgcttg tattttgctt tcgaggagtc
      361 cccgagtcag atcgtccgca atatgaaatc gatcgggatc aaccttcaac cctggttgga
      421 gaaagggctc ctagagatcg atgccgcccg gccttcacac tatggactcg aaacgcatct
      481 tgccggaata cagaaacgta tactggaggt aaagccgcat gcggtgatcg tggacccgat
      541 cactaatttc actaccggca acacggccat cgatgtcaaa gctatggtga gtcggctcat
      601 tgatttctta aaatcacaac agatcacgac aatgttcgtc agcctgacag gcggaggaga
      661 aatgatagag tccaccgagg taggcatttc ctcattgatg gacgcctgga tcattcttcg
      721 agacatggag gtcaatgggg agcgaaatcg cgtgctctat cttgttaaag cccgtggggt
      781 ggcccattcc aatcaagttc gtgaatttgt cctgacaagc aaaggaatcc aattggtaga
      841 cgcttacctg gggggcaaca ccgtcctgac cggatcggcg cggctggctc aaaaggcgca
      901 ggagcggtta atggatctcg aacacaaaca ggaggctgag gcgacccagc gcaggatcga
      961 acgcgagcgc caggtcctcg aaacccagat cgcaacattg caggtggaat acgaagccaa
     1021 aaaagatgag ctgaagcgag caatggggaa agagcagtta aaggaaacaa tgacgattac
     1081 cgatcaagca acgatggaac agagccgaaa ggtctctgtc cgcaattcca ggccgaacaa
     1141 gaaatatgca aaaagataga caaatgcgtc cagcccgaaa aaaagatgct tctccggtat
     1201 atctcaagct ttacatagcc gggcagacgc ctaagtcggt gatcgctctg gccaacctga
     1261 agaaaatttg cgatgagcat ctggccggta aattcaacat cgaggttatt gatcttctta
     1321 agaacccaag cctggcccaa ggagaccaga ttcttgcgat tccgacgctg atcaggaagt
     1381 tgcctcaacc catacggaag attatcggaa atctatccaa tacggagcgg gtactgatcg
     1441 gtttggactt ggagtcccgg gttacgatat gaaccaccaa aaagtaatac ggcatttagc
     1501 cgacaagatt gccaggaaaa ccaatgccaa gagacctaag tataccctga gtttatatat
     1561 taccgggacc acgccccaat caacccgtgc gatcctcaat cttaaacgaa tctgcgatca
     1621 gtatctgaag gggcattata tactcaagat catcgacatc tatcagcaac ctcatctggc
     1681 caaagatgcg cagatcatcg ccgccccgac gctcgtcaaa aaattaccaa ttcctcttcg
     1741 ccgattcatc ggcgatatgt cgaatgagga acacctgttg atcgggttag acctccaacc
     1801 gagggatgaa aacggctcac gggctaagaa gggaggtttg aaaaatgaat aagcagtccc
     1861 ggaaaaatta cagcgccaaa gacctattgc ataatatcga agaactccaa cgccaactga
     1921 gcgaagccca tgaaacgctg gacgctattc ggagcggggc cgtggacgga atcgtcgtgt
     1981 cg
//
LOCUS       DATMKH010000306         1662 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_143519,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000306 DATMKH010000000
VERSION     DATMKH010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143519"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="VNV63_03725"
     CDS             <1..467
                     /locus_tag="VNV63_03725"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXC61762.1"
                     /translation="LEQGITVAIEHSNGLSRVIADREQLKTCFINITANAIQAMPQGG
                     ELKVETEMDKRQEEIRISFSDTGHGISEESLQRIFQPYFTTKRLGIGLGLALTQRIIH
                     EHGGQITVSSQLNHGTRVVVTLPVNRKPELKSHDRDPSMGRGDVDPVMEVKG"
     gene            464..>1662
                     /locus_tag="VNV63_03730"
     CDS             464..>1662
                     /locus_tag="VNV63_03730"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXC61763.1"
                     /translation="MKTGNVLVVDDEKSQRDIVKTILKSADYDVETAANGQQAGELYR
                     TGNFDVVLTDLKLPDTDGLSIVDQMFQIQPLGCLIIMTAHGSIDSAVEAMKKGAFDYL
                     TKPLDRDQILIVLKRAFEKVQLLKENRLLHQQLEERFSLDNIIGHHNKMQELFQIIKK
                     ISTSDVTVLICGESGTGKELIARAIHFNSQRKHKPFMAINCAAIPEPLIDSELFGYEK
                     GAFTGAAIRRIGLFEATDKGTLFLDEIGDLSLALQAKLLRTLQEREVRRLGGREQIKI
                     DVRVIAATNKQLEAEIRNGNFREDLFYRLNVMAIQVPPLRERSTDIPALVEHFIQKYT
                     SKVVKSVKEISHEAMRFLMEYSWPGNVRQLESVIERVILLCETDRIELVDLPSEIKTG
                     PVPITGF"
BASE COUNT          438 a          386 c          445 g          393 t
ORIGIN      
        1 cgcttgaaca gggcattacc gttgcaatcg agcattcgaa tggcttgtct cgggtcatag
       61 cggatcgcga acagctcaag acctgtttta taaatatcac ggccaacgcc attcaggcga
      121 tgcctcaggg cggcgagcta aaagtcgaaa cggagatgga caagagacag gaggagatca
      181 ggatcagctt ttccgatacg ggacacggga tcagcgaaga gtccctccag cggatctttc
      241 aaccttactt tacgaccaaa cggctgggca tcggcctcgg gcttgccctg actcagcgaa
      301 tcattcatga acatggcgga caaattactg tttcaagcca gctgaatcac ggaacgcggg
      361 tggtagttac cttgccggtc aatagaaagc ctgaactaaa gtcccatgat cgagatcctt
      421 cgatgggccg cggagacgtt gatcctgtca tggaggtcaa gggatgaaga ccgggaacgt
      481 cctggttgtg gatgatgaga agtctcaacg ggatatcgtg aagacaatcc tgaaatcggc
      541 cgactatgat gtcgagaccg ccgccaacgg tcaacaggcc ggcgaactgt accgaacggg
      601 taattttgat gtcgtgctga cggatctgaa gctccccgat acggatggtc tatcgattgt
      661 ggatcagatg tttcaaatcc agccgcttgg ttgcttgatc atcatgacgg cccatggttc
      721 cattgattcg gccgtagagg cgatgaagaa aggggccttt gattatttga ctaagccttt
      781 agatcgagat cagatcctga ttgttctgaa acgggccttt gagaaagtgc aactcttgaa
      841 agaaaacagg ctgctccacc agcagttgga agagcgcttc agcctggata acatcattgg
      901 ccatcacaat aaaatgcagg aattatttca aatcattaaa aagatctcaa ccagcgatgt
      961 gacggtgcta atctgtggtg aaagcggtac cggcaaggag cttattgccc gcgcgattca
     1021 tttcaacagc cagagaaaac ataaaccttt catggcgatt aattgtgcag cgattccgga
     1081 accattgatt gatagcgaac tcttcggcta tgaaaaagga gcctttaccg gagccgctat
     1141 ccgtcgtatt ggtctcttcg aggctacgga taaaggcaca ctgtttttag atgaaatcgg
     1201 cgatctctcg cttgctttac aggccaagct tcttcgaacc ctgcaggagc gtgaggtccg
     1261 acgattggga gggcgtgagc agatcaaaat agatgtccgt gtcattgctg cgacaaacaa
     1321 gcaacttgaa gcagaaatcc ggaatgggaa ttttcgagag gatctctttt atcggcttaa
     1381 tgtcatggcc attcaggttc ctcctctgcg ggagcgcagc accgatatcc cggctctggt
     1441 ggagcatttt attcagaagt acaccagtaa agttgtaaag tccgttaagg agatcagtca
     1501 cgaagctatg cggtttctta tggaatactc ctggccggga aatgttcgac agcttgaatc
     1561 ggtcatcgaa cgcgtcattc tcctatgcga gacagatcgc atcgagctgg tggatctgcc
     1621 atccgaaatc aaaacggggc cggttccaat cacgggtttt ga
//
LOCUS       DATMKH010000307         2299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3649647, whole genome shotgun sequence.
ACCESSION   DATMKH010000307 DATMKH010000000
VERSION     DATMKH010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3649647"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1267)
                     /gene="glgP"
                     /locus_tag="VNV63_03735"
     CDS             complement(<1..1267)
                     /gene="glgP"
                     /locus_tag="VNV63_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006889804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HXC61764.1"
                     /translation="MSNERRVRNPIYTSLSAEVEGFDSLAELALDMRWSWNRYADEVW
                     RRLDPTLWDLTHNPWVVLQTVSRAQLQRVLADPSFRKEIDDLVQIKRRMAETPAWFQQ
                     HHSQSPLTCVGYFCMEFMLNEALPIYSGGLGNVAGDQLKASSDLGVPAVGVGLLYQQG
                     YFRQVIDKDGAQQALFPYNDPGQLPIRPLREQNGEWLRLEIALPGYSVWLRAWQVQVG
                     RVKLYLLDSNDAANFPAHRGITSVLYGGGPELRLMQEMVLGIGGWQLLEKLGLRPEVC
                     HLNEGHAAFAVLERARSFMEETGQPFQVALAVTRAGNLFTTHTAVAAGFDRFAPALIE
                     QYLRGYAEKRLGITLHDLLALGRQNPNDSSESFNMAHLAIRGSGVVNGVSDLHGKVSR
                     RIFAPLFPGWPEDEVPVGHVTNGVHTPSWD"
     gene            1465..>2299
                     /locus_tag="VNV63_03740"
     CDS             1465..>2299
                     /locus_tag="VNV63_03740"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HXC61765.1"
                     /translation="MIKMTRKLFYGIGLGFVILLLIGLLWAWSVVSSLPDVSSLRNYK
                     PQPVSEVYDRNGRAILQIPQNPSHLWVPLSEISESLQMAVITMEDDTFFQHKGINYKE
                     TWNAFKEDLKKGKYKRGGSTITQQLAKNLFLSKEKTLTRKLKEYFLARRIEEILPKER
                     ILELYLNEAEWGDKIYGAEPAARFYFDKHASELNLPESALLAAMLSNPHYYNPFKNLE
                     RIQNRQKRLFSLLRTDHMINDEEYQEALQAPLVLQNPEVAPQPKAAMSSSKDSPPPAC
                     HL"
BASE COUNT          559 a          632 c          583 g          525 t
ORIGIN      
        1 agtcccaact cggcgtatgg actccgttgg tcacatgccc gaccggcact tcgtcctccg
       61 gccaccccgg aaagagaggc gcaaaaatgc gccggctcac tttcccatgc agatcactca
      121 cgccattgac caccccgctc ccgcgaatcg caagatgtgc catgttgaaa ctttccgatg
      181 agtcatttgg gttctggcgg cccagcgcca gcaaatcgtg gagtgttata ccgagccttt
      241 tctcggcata accacggagg tattgctcga taagcgccgg tgcaaaacgg tcaaagcctg
      301 cggccactgc cgtatgtgtc gtgaagagat tgccggctcg cgtgacggcc agtgctactt
      361 ggaagggttg tcccgtctct tccatgaaac tccgggcgcg ttccaatacg gcaaaggccg
      421 catgcccttc attcaggtgg cagacttccg gccggaggcc gagtttttcc aggagctgcc
      481 atccgccgat cccaagaacc atctcttgca tgagacgcag ctccggcccg ccgccataga
      541 gcacgctggt aatccctcga tgagctggaa agtttgccgc gtcattgctg tccaagagat
      601 aaagcttcac tctgccgacc tggacctgcc aggcgcgcag ccagacggag taaccaggca
      661 aggcgatctc caaccgcaac cattccccgt tctgctcacg caaaggccta atcggtaatt
      721 gccccggatc attgtatggg aaaagagcct gctgcgctcc gtctttatca atcacctggc
      781 gaaaataacc ctgctggtag agtagcccca cgccgaccgc cggcacgccc agatcgctgg
      841 aggctttgag ctgatcgccg gccacattgc caaggccgcc tgaatagatg gggagagctt
      901 cgtttaacat aaattccata cagaagtacc cgacgcatgt caggggagac tgcgaatgat
      961 gttgctggaa ccacgcgggc gtctccgcca tgcgacgctt aatttgcaca agatcatcga
     1021 tctctttgcg aaaagaagga tcggccaaca cgcgctggag ctgggcacgc gagacggtct
     1081 gcaagacgac ccaggggtta tgagtgagat cccacagcgt tggatcaagc cgccgccaca
     1141 cttcatcggc gtaacgattc cacgaccagc gcatatcgag ggcaagctcg gccagggaat
     1201 cgaatccctc aacttctgca gatagagaag tgtatatcgg atttctgacg cgtctttcgt
     1261 tgctcatggt ctatttttta ttctcaaata atataatccg tttatctctg agagaaaatt
     1321 acacgttttt aaagaacctg tcaatcgttt gttaggggaa aaagctcctt ttccttgaaa
     1381 ctccgtctcg ttttcggtta tgatagcgtt cgctaatttt atacggcggt gatgatcccg
     1441 tgatgataca acgatgatgc aacaatgatc aagatgacaa gaaagttatt ttacggtatc
     1501 gggctcgggt ttgtgatctt gctattgatc ggtctgctct gggcctggtc tgttgtcagt
     1561 tcgcttccgg atgtctccag tcttcgtaat tacaagccac agcctgtgtc ggaggtttat
     1621 gaccggaatg gacgagcgat tctccagatc ccacaaaacc catctcacct ctgggtgccg
     1681 ctctccgaga tctcggagtc gctgcagatg gccgtaatca caatggagga tgataccttc
     1741 tttcaacaca agggcattaa ttataaagaa acctggaacg cctttaaaga agatctcaaa
     1801 aagggaaaat ataaacgcgg tggaagcacg atcacgcagc agcttgccaa gaatctattt
     1861 ctttcgaagg agaaaaccct tacacgaaaa ttgaaggaat attttcttgc ccggaggata
     1921 gaggagatcc tccccaagga gcggatcttg gaactctatc tcaatgaggc ggaatgggga
     1981 gataaaattt acggcgcgga gccggccgct cgtttctact ttgataaaca tgcatccgag
     2041 ctcaatttgc cggagagcgc tctcttggcc gcgatgctgt cgaatccaca ttactacaat
     2101 cccttcaaga atctggagcg tatccagaat cggcagaagc ggctgttttc tcttctccga
     2161 accgatcaca tgatcaatga tgaggaatat caagaagcct tacaggcgcc gctggttctg
     2221 caaaaccccg aggtagcacc gcagccgaaa gcggccatgt cttcttcaaa ggattccccg
     2281 ccgccggcat gccaccttc
//
LOCUS       DATMKH010000308         1132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4313097, whole genome shotgun sequence.
ACCESSION   DATMKH010000308 DATMKH010000000
VERSION     DATMKH010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4313097"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VNV63_03745"
     CDS             complement(<1..203)
                     /locus_tag="VNV63_03745"
                     /inference="COORDINATES: protein motif:HMM:NF024886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase N-terminal SAM-like
                     domain-containing protein"
                     /protein_id="HXC61766.1"
                     /translation="MTPLRAKYIQDLGIRGRSENTQEVYTRYVCDLARYYRRSPELIS
                     YEEVTGWLYYLIQERQLSASSVN"
     gene            477..737
                     /locus_tag="VNV63_03750"
     CDS             477..737
                     /locus_tag="VNV63_03750"
                     /inference="COORDINATES: protein motif:HMM:TIGR02574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HXC61767.1"
                     /translation="MRDGPLYGTLVDMIKETIPKLQKLSTADKFALAVELWDELSSNP
                     DEIPVTEEQLNELDRRFEEYRKDPDKVVTWEDAKARILSGRR"
     gene            737..1033
                     /locus_tag="VNV63_03755"
     CDS             737..1033
                     /locus_tag="VNV63_03755"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HXC61768.1"
                     /translation="MEVVLLLSAERDLQEAYNWVEEHRQGREQYFLQDVDLRLEHLKM
                     FPLIGRLYRSRYRRLLIPRYPFGIFYVVESNRIVIHAVLDLRQDPKKIQERFSE"
BASE COUNT          267 a          291 c          288 g          286 t
ORIGIN      
        1 atgttgacgc tggatgctga cagttggcgt tcctggatca gatagtacag ccagcccgtg
       61 acttcctcgt aggagatcag ctctggcgaa cggcgataat agcgtgccag atcacagacg
      121 taccttgtat agacttcctg agtgttttct gagcggccac gaatgcccag gtcctggatg
      181 tatttggctc gtaagggtgt cataggtcgg ccaatatgcc gaccatgact tcaccgctca
      241 cgcccgccac tcacgactcc gcgtagcggc ttcgttcaac ctgcgttccg atggcctatt
      301 gcgaataaga tgtcttcata agtggctgta aatcaggcaa gtccatcaac agaaaaactt
      361 gcgccataag aagaatccgt tcggtgtaac accgcatctc cagcgcaatc gatgtctgct
      421 ccggtaaaag cggaagtacg atagagcccc gcccagaccg ggacgctttc ccccaattgc
      481 gtgatggccc gctgtacggt actctggtcg acatgatcaa ggagacgatt cccaagctgc
      541 aaaagctttc gaccgccgac aagtttgcct tggcggtcga gctttgggac gagttatctt
      601 ccaatcccga cgaaatcccc gttactgagg agcagctgaa cgaactcgat aggcgttttg
      661 aagaatatcg gaaagatccg gataaagtcg tcacctggga agatgcgaag gcaaggatcc
      721 tttccggtcg gcgttaatgg aggttgtcct tcttctctcc gcagaacggg atcttcaaga
      781 ggcgtacaac tgggttgaag agcaccgtca agggagagaa cagtatttcc tgcaggatgt
      841 agacttgagg cttgagcatc tcaaaatgtt tcctcttatt gggcggcttt atcggagccg
      901 ataccgccgt ttgctcatcc cgagataccc ttttggtatc ttctacgttg tcgaatcaaa
      961 caggattgtt attcatgcgg tcctcgatct ccgccaagat cccaaaaaga tccaagagcg
     1021 cttttccgaa tagggattgt tttattttga tctgcctcct ggctgccctc aaatgcgttt
     1081 gtaagcaatt tttttcgtct ttcaattaaa tcgtagactg atgcagcgcc gc
//
LOCUS       DATMKH010000309         1161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4313856, whole genome shotgun sequence.
ACCESSION   DATMKH010000309 DATMKH010000000
VERSION     DATMKH010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4313856"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /gene="infC"
                     /locus_tag="VNV63_03760"
     CDS             complement(<1..362)
                     /gene="infC"
                     /locus_tag="VNV63_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HXC61769.1"
                     /translation="MPEVRAIGPEGEQLGVLPIRDALKKAEELGYDLVEIAPTAKPPV
                     CRIMDYGKYKYEQSKKQHTAKMHQRSMLVKEIKLRPQTDKHDLEFKIRHMREFLEEGH
                     KVKVTLMFRGREMTYLDRG"
     gene            complement(596..>1161)
                     /locus_tag="VNV63_03765"
     CDS             complement(596..>1161)
                     /locus_tag="VNV63_03765"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005007184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HXC61770.1"
                     /translation="PYKTDPGEGVFYGPKIDIKIKDVLGRAWQCTTIQVDFNLPERFA
                     LTFIGEDGKEHTPIMLHRALLGSLERFVGVLIEQHAGAFPAWLAPVQAKVIPISESQH
                     AYANEVMGQLTDATIRAEGDFRNETMGLKIREAQLAKVPYMLILGKREFASKTVAVRK
                     RTGEDLGEKSLEQVLGMIQEEVRTRRN"
BASE COUNT          219 a          355 c          278 g          309 t
ORIGIN      
        1 ccccggtcca gataggtcat ctcacgccct cggaacatga gtgttacttt gaccttgtga
       61 ccctcttcca gaaattcccg catgtgacgt atcttaaact ccaaatcatg tttatctgtt
      121 tgcggcctca gcttgatctc cttcaccaac atcgatcgct gatgcatctt cgcggtgtgc
      181 tgctttttgc tttgctcgta tttgtatttt ccgtaatcca tgatccgaca gaccggcggt
      241 ttggcggtcg gagcaatctc aaccaggtca taccccaact cttcggcctt ctttaaggcg
      301 tcccgtatgg gtagaacgcc caactgttct ccttccggcc caatcgctcg gacctcaggc
      361 attcggatct ctctgttgat acggagttta gaagcgatca gctttctcct ttctcttttt
      421 tggattctat catagggctc gcgtcgctgc tattatctat gggggcccgt gcggccaaac
      481 aacggtcctc cgatgccccc atgccccgcg ctcggacggg cagagcccga tccttgcttt
      541 cccactatag cctccccctc tcgctcgcct tgctcgctgg gaggtattcg gtccgtcaat
      601 ttctgcgtgt ccggacctct tcttgaatca tgccgaggac ctgctcaagc gatttttctc
      661 ccagatcttc accggttcgc ttgcgaactg ctacggtctt cgaagcaaac tcccgtttac
      721 ccaggatgag catgtaaggg actttggcca gctgggcttc ccgaattttc aaccccattg
      781 tctcattgcg aaaatctccc tcggcccgga tcgtcgcatc ggtaagctgt cccatgacct
      841 cattggcata cgcgtgctgt gactcggaaa tcggaatcac ttttgcctgc accggggcca
      901 gccaagccgg aaaggcccct gcatgttgct cgatgaggac tccgacaaac cgttcgagcg
      961 aaccaagaag ggcccggtgg agcatgatcg gcgtgtgttc tttgccgtct tccccgataa
     1021 atgtcagggc gaaacgttcg gggaggttaa aatccacctg gattgtagtg cactgccagg
     1081 cccggcccaa gacatccttg atcttgatat caatcttggg accgtagaaa accccctcac
     1141 ccggatccgt cttgtacggg a
//
LOCUS       DATMKH010000310         1926 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3977415, whole genome shotgun sequence.
ACCESSION   DATMKH010000310 DATMKH010000000
VERSION     DATMKH010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3977415"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="VNV63_03770"
     CDS             <1..389
                     /locus_tag="VNV63_03770"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HXC61771.1"
                     /translation="QGLCFAIAANTTQLVAGWLIKDGKIRRSYLGLAGQNVPLHPKVT
                     RFHRLEQESGVLIAGIEQGGPVDLSGLKEGDIILSFKGNPVSSIDVLHRLLVGSEIGQ
                     RSVLSVIRGTEMLDFLVTPRELPAGG"
     gene            626..1075
                     /gene="rpiB"
                     /locus_tag="VNV63_03775"
     CDS             626..1075
                     /gene="rpiB"
                     /locus_tag="VNV63_03775"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008104723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HXC61772.1"
                     /translation="MKISIGSDHAGFLYKEKIKAMLTEMGHEVLDFGTDSEEPCDYPL
                     FIRPAAEAVARGQAERGIVPGGSGNGEAITANRIPGIRCGLCWTEQVAKWHRLHNDGN
                     VLSLGQRTISEEEALNIVKVWLDTPFEGGRHVRRIYLIDHPEAAVAS"
     gene            1078..1545
                     /locus_tag="VNV63_03780"
     CDS             1078..1545
                     /locus_tag="VNV63_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61773.1"
                     /translation="MSFEQQIRAAEGYAELGMYSDALTELDQLPPEDQERLESLRMRV
                     QVVLQTRDWNSGLKLSRRIAEIFPNESYGFIHAAFCLHELGEAQRAKETLLNGPPSLL
                     EEPIYYYNLGCYETVLGNLDQAKAYLRASFRLNKGFREIARQDPDLAPIRDEL"
     gene            1648..>1926
                     /locus_tag="VNV63_03785"
     CDS             1648..>1926
                     /locus_tag="VNV63_03785"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HXC61774.1"
                     /translation="MTLIDLFEENFTKYGELGASVCLWQDGKMAASLAGGFWDQAKTR
                     KWTTETRVLIWSATKGLASTCLLHLCEREKIALTRQVADFWPEYAENGK"
BASE COUNT          455 a          499 c          533 g          439 t
ORIGIN      
        1 ctcaaggact ctgctttgct attgccgcca atacaactca actcgttgcc ggctggctga
       61 tcaaggacgg taagattcgc cggagctacc tgggacttgc cggtcagaat gtgccgttgc
      121 accccaaggt cactcgcttc caccgactcg aacaagaatc cggtgtcttg attgccggca
      181 tcgaacaagg aggacccgtc gatttatcgg gtctgaaaga gggtgacatc attctttctt
      241 ttaaaggcaa tccagtaagc tcaatcgatg tcctgcaccg gcttttggtc ggtagcgaga
      301 tcggtcaacg atcagtgttg tccgtgatcc ggggcactga aatgctcgat tttctcgtta
      361 cacccaggga attgccggct ggaggctagg ggctagagaa aggagttcag gagttcggga
      421 gccagcgagc gaccgtgctg gcctagtatt acctcgtcac gttcagctta cccctttgtc
      481 cttgcaattt tctgcttctc cctagcctga actcctgaac tcctgaactc ctgaactcct
      541 gaactcctga actcctgaac tcctgaactc ctgaactcct aaaaaacctt tgatccgaac
      601 cgatcatcat cgaagagttg ggcctatgaa gatctctatc ggctctgacc atgccgggtt
      661 tctctataaa gagaagatta aagcaatgct cacggagatg ggccatgagg ttttggactt
      721 tgggacagat tccgaggagc catgtgatta cccacttttt atcaggccgg cggcagaagc
      781 cgttgctcgt ggtcaggcgg aacgcggcat cgtgccagga ggatcgggaa atggggaagc
      841 gattacggct aaccgcattc ccggcatccg ctgtgggctc tgctggaccg agcaggtggc
      901 taaatggcat cgattacata atgacggcaa cgtcctatcg ctggggcagc gcacaatttc
      961 tgaggaagaa gcgctaaata tcgtcaaggt ttggctggat acgccgtttg aaggggggcg
     1021 acacgtgcgc cgcatttatc tgatcgatca tcctgaagca gcggtggctt cctgaggatg
     1081 agttttgaac agcagatccg agccgcggag ggttacgcgg aactcgggat gtattccgac
     1141 gccttaacgg aactggatca gctcccgccg gaagatcagg aacgcctgga gagtctccgg
     1201 atgcgggtgc aagtcgtttt gcaaaccagg gactggaact ccggcttaaa acttagtcga
     1261 aggatcgccg aaatctttcc caacgagagc tacggattca tccatgcggc tttctgttta
     1321 catgaactcg gcgaggccca gagagcaaag gaaacccttt taaatggccc tccctcattg
     1381 ttggaggaac ccatttacta ttacaacctc ggttgttacg agactgtcct cggcaacctc
     1441 gatcaagcaa aggcgtacct cagagccagc tttcggttaa acaagggctt ccgcgaaatc
     1501 gcccgccagg atcctgatct agccccgatt cgtgacgagc tctaaagggt tcaccgttcg
     1561 cggttcacag ttcgcggttc agccaagggc ttggaacagt aaacggtgaa ctgcgaacag
     1621 tgaaccgtga accgcgaagc gtcacccatg actctgatcg acctttttga agaaaacttt
     1681 actaaatatg gcgaactagg cgcatcggtt tgcttatggc aggacggcaa aatggctgcg
     1741 tcgctcgccg gcggtttctg ggaccaggca aagacgagaa agtggacaac cgagacccgg
     1801 gtgctgatct ggtcggctac caaagggttg gcttccacct gtcttttgca tctgtgcgaa
     1861 cgagagaaaa tcgcgttgac ccgtcaagta gcggactttt ggccggagta cgcagagaat
     1921 gggaaa
//
LOCUS       DATMKH010000311         2052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1425588, whole genome shotgun sequence.
ACCESSION   DATMKH010000311 DATMKH010000000
VERSION     DATMKH010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1425588"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..1185)
                     /locus_tag="VNV63_03790"
     CDS             complement(13..1185)
                     /locus_tag="VNV63_03790"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HXC61775.1"
                     /translation="MEIFRLKRHQERRVLRGHPWIFSNELTRLTSDMEPGTLVEVQDP
                     LGRFIGRGYFNPHSLIAVRLLTRTNQAIDSDFFIQRIHQADLFRKRIFPEERSYRVVF
                     SEADGLPGLIVDRYENNIVLQITTAGMERWISTICDVLVEMFKPKTIVARNDTAIREL
                     EGLKLEKRVLYGSVESSTVITKNGLEFELDLLEGQKTGFYLDQSENYAKLQGLVTGGT
                     VLDGFCYAGGWALHAVRYGAKKVIGLDSSEKAIGLAMANARRNGLENRCDFRKADLFD
                     ELRKQVSRGDRYDGIVLDPPAFIKSKTKLKEGLRGYREINRCAMELVKPGGILVTCSC
                     SYHLDREGFRKMLSQAAQEAGRTTRLLEFRSQSRDHPVLLSIPETDYLKCAILMIE"
     gene            complement(1239..1667)
                     /locus_tag="VNV63_03795"
     CDS             complement(1239..1667)
                     /locus_tag="VNV63_03795"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HXC61776.1"
                     /translation="MIRRIGAAFILSLLIAVTFEAAANERPPSVHPQQVTVRLREYRF
                     EPDQITLKAGQETELILVNEGSVLHEFITEVLQNLTVNVEVNGVVAETLGLAELEIPP
                     KEKVVLRFTTEKSGEFPIFCRAIAPKDHYKAGMVGRLVIR"
     gene            complement(1669..>2052)
                     /locus_tag="VNV63_03800"
     CDS             complement(1669..>2052)
                     /locus_tag="VNV63_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61777.1"
                     /translation="IISLLRVGPGYQDIVTHYRGSGTEEAFPRAFGQMLEEAHFHSFI
                     EGVTLLVLTHLFIATSVKRSVKTGTILLAYSATLTDLACPWLIRYVSSGFALLQIVSW
                     VVMGATALALIGIPFYEMWLMKGDQ"
BASE COUNT          506 a          587 c          464 g          495 t
ORIGIN      
        1 aattcaatcc atttactcga tcatcagaat tgcgcatttt aagtagtccg tctccgggat
       61 cgagagcaga acaggatgat ccctcgattg cgaacgaaac tcgaggaggc gggttgtgcg
      121 tcctgcttct tgtgccgcct ggctcagcat ctttcgaaaa ccctctcgat ccaggtgata
      181 cgagcaggag cacgtcacga gaataccgcc cggcttgact aattccatcg cgcaacgatt
      241 gatctctcga taaccccgca ggccctcttt caatttggtc ttgctcttga taaaggccgg
      301 cggatccagg acgatgccgt cgtaccggtc acccctcgaa acctgcttcc taagctcgtc
      361 aaagagatcg gcctttctga aatcacaccg attttctaag ccattccgcc gggcgttggc
      421 cattgccaag ccgatggcct tctcggagct gtccagaccg atgacttttt tggcaccata
      481 acgcaccgca tgcaaggccc agccgccggc atagcagaaa ccgtccagca ccgtgccgcc
      541 ggtaacaagg ccttggagct tggcatagtt ctcgctctga tccagataaa atccggtctt
      601 ctggccttcg agaaggtcca gctcaaattc aagaccgttc ttggtgatga ccgtcgacga
      661 ttcgacactg ccgtagagaa cccgtttttc tagcttcaaa ccttcaagct ctcgaatcgc
      721 tgtatcgttc cgagctacaa tcgtctttgg tttgaacatc tccaccaaga cgtcacagat
      781 cgttgagatc caccgttcca tcccggccgt cgttatttgc agaacgatgt tgttttcata
      841 ccggtccact atcaaacccg gtaagccatc cgcctcgctg aacacgacac gatatgaccg
      901 ctcctccggg aagatccgtt ttctgaacaa atccgcctga tgaatccgct ggataaagaa
      961 gtctgagtca atcgcttgat tggttcgagt aagcaggcgg accgcgatca aagagtgtgg
     1021 gttaaagtag ccgcggccaa taaagcggcc cagaggatcc tgcacctcca ccagcgtacc
     1081 gggctccata tccgacgtca ggcgggtaag ttcattgcta aagatccagg gatggccgcg
     1141 aagaacccgt ctctcctggt gtcgttttaa ccgaaaaatc tccatgacgc taaatccaat
     1201 cccactaatt ttttcacagc tgtcgggttt ccccttattt aacggatcac cagtcttcca
     1261 accatgcctg ctttataatg atccttaggc gctattgccc tgcagaaaat cggaaattcc
     1321 ccggattttt ctgtggtaaa gcgaagaacg actttttctt tcggagggat ctccagctcc
     1381 gctagtccga gggtctcagc cacaacaccg ttgacctcca cattcactgt cagattttgt
     1441 aacacctcgg ttatgaactc gtgtaagact gacccttcat tcaccaggat cagttcagtc
     1501 tcctggcccg cttttaatgt gatctggtcc ggctcaaacc ggtactctcg caagcgcacc
     1561 gtgacctgct gagggtgaac gcttggaggt ctttcattag cggcggcttc aaaagtcacc
     1621 gcgataagca aagacaggat gaacgccgct ccaattcgtc ttatcatttc attggtcgcc
     1681 tttcattaac cacatctcat aaaacggtat tccaatcaaa gccagagcag ttgcacccat
     1741 cacaacccaa cttactattt gcaatagagc gaacccagac gatacatacc ggatcaacca
     1801 gggacaagcc agatccgtca aagtggcact gtaggccaga agaatcgttc ccgtcttgac
     1861 agatcttttc accgaagttg ctataaataa atgtgttaaa accagtaaag taactccctc
     1921 gatgaatgaa tgaaaatggg cctcttccag catctggccg aaagcccggg gaaatgcctc
     1981 ttctgtcccg gaaccgcggt agtgagtgac aatatcctga tagcccggtc caacccgaag
     2041 cagactgatg at
//
LOCUS       DATMKH010000312         1524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_146681,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000312 DATMKH010000000
VERSION     DATMKH010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146681"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1513
                     /locus_tag="VNV63_03805"
     CDS             <1..1513
                     /locus_tag="VNV63_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61778.1"
                     /translation="GGVAQVFIMLISGGEPLQLTNDNGDKVVNSFSPDGTEIFYRTEI
                     GTNDVWIVPTLGGNPQRLSLGYWVTPSLDGLWLYYVKVGNRVSIFRSDKSGLGEEEVY
                     HFEAPALRPVRILPYSSEDRLLVFLSNPQSAIQQFKVYDVDPHSQMAKELDEVSANPL
                     DAVWAEPGKTVLLSRTVNGLTNIWKYNLKDKSLTQITFGTGPDFSPMLDPDGKGIYFL
                     NGKSSGLLTAYNARSKESTDIVEDATQPAISPNGKRLMYVTVPDQERTELWVSNIDGS
                     KKLKLATATSGSLTTGSWAPDNLHLTFMEEEASGKPDKPYVVAADSGRPRELSGTGVV
                     IQNIQWSADQKSIYLNGLGASGWTIWRENVDGSNLEKFVEGCGEAFDVASNGEYLLNL
                     APAGEKTGIYEFSIADRRCTLLLPGVVTFGARFAPDGKSFSYAVQSEGRVTIYRQAWS
                     DGALVGKPQIALKLPFSFPLQYGGNAYDFSPDLSTVAYARPGGHADLYLLSQK"
BASE COUNT          362 a          392 c          413 g          357 t
ORIGIN      
        1 ggggggagtg gcgcaggtat tcatcatgct catttccgga ggcgagcccc ttcaacttac
       61 caacgacaat ggcgacaaag ttgtgaacag cttctccccc gatgggaccg aaatctttta
      121 ccgaacagag atcggcacaa acgatgtttg gatagtgcct accctcggtg ggaacccgca
      181 gcggttgtcg ctcggctatt gggtaactcc ttctctggac ggtctttggc tttattacgt
      241 gaaggtaggg aacagggtaa gcatatttcg ttcggacaaa tccggattgg gagaggaaga
      301 ggtctatcat tttgaggccc ccgcccttcg tcccgtacgg atcctgccat actctagtga
      361 agaccgtctg ctggtcttcc tttcaaatcc tcaatctgct attcagcagt tcaaggtcta
      421 cgatgtggat ccacactctc agatggcgaa ggagttggat gaagtatcag cgaaccctct
      481 agatgccgta tgggcagagc ctggaaagac cgtgttgctg agccggacgg taaacgggtt
      541 gaccaacatc tggaagtaca atctgaaaga caaatcgctc actcagatta cctttggaac
      601 cggaccggat ttttcgccaa tgctcgaccc cgacggaaaa gggatttatt tcctgaatgg
      661 taagtcctcc gggctcttaa ccgcctacaa cgcgcgttcg aaagaatcca cggacattgt
      721 ggaggatgcg acacagccgg cgatctcccc gaacgggaaa cgccttatgt atgtcacagt
      781 gcccgatcag gaacgaactg agctctgggt gtcgaacatt gatgggagca agaaattgaa
      841 actcgccacg gccacatccg gttcgctgac cacagggagt tgggcgcctg acaatcttca
      901 tctcactttt atggaggaag aagcatccgg gaaaccagac aaaccgtacg tcgtcgctgc
      961 ggatagtggc cggcctcgcg aactttccgg gacaggcgta gtcatccaaa atattcaatg
     1021 gagcgctgat cagaaatcca tttacctgaa tggtctaggc gcctcggggt ggacaatttg
     1081 gagagaaaac gtggatggat cgaacctgga aaaatttgtc gagggctgtg gggaggcatt
     1141 cgatgtggcc tcgaatggag aatatttgct taatttagca ccggctggag aaaagaccgg
     1201 aatctacgag ttctctatag ccgatcgcag gtgcaccttg ttgttgcccg gtgtggtaac
     1261 ttttggagct cgatttgccc ccgatggcaa atccttctcg tatgccgtcc aatcggaggg
     1321 aagggttaca atctaccgtc aggcctggag cgacggcgcg ctcgtcggaa agcctcagat
     1381 tgcgctaaag ctcccttttt catttccact ccagtacggt ggtaatgcct acgacttctc
     1441 cccagacctt tccactgttg cgtacgctcg gcccggcggt cacgccgacc tctatcttct
     1501 gagccagaaa taaatttctt cgcg
//
LOCUS       DATMKH010000313         1033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_468308,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000313 DATMKH010000000
VERSION     DATMKH010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468308"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1033
                     /locus_tag="VNV63_03810"
     CDS             <1..>1033
                     /locus_tag="VNV63_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61779.1"
                     /translation="WHLFYDGKGVISAVAKGKGATLSWSKGPAMEALELGKADRFQAL
                     AQEVKVSMRDQTSLGIVLHLADEIDLAIVQEGFANPELFEQARALVRETPSEVVTDLS
                     TDQDPAIQWRYYPLLSGQRAIVLRHQIEFFSAMQTLTALDIKVAVHSAPIEMLAIYLK
                     LYAEAIEEKPHCFVFFYDRFTVVIPANQGVLDVKVLPHRQQDVPPAFGDDLFSLLERF
                     GLVDSCVLLLVQCGTQDPTLLFRELDTYARRNKKNANGIEIQIPDKEIVWKAFDEHTP
                     GRVKEEIIHRPEFLTEYNERSGKDFPLSLGIKSDAQRFGILCRETFWPDDQKSRDQRL
                     PRSLALTITA"
BASE COUNT          244 a          271 c          282 g          236 t
ORIGIN      
        1 ctggcatctc ttttatgatg gaaagggagt catctcggcc gtagccaaag ggaaaggcgc
       61 gacgctatcc tggtcgaagg gaccggcgat ggaggccctt gaactcggca aagcggatcg
      121 tttccaggcc ctggcgcaag aggtgaaggt ttctatgcgc gatcagacca gcctcggcat
      181 tgtccttcac ctcgccgatg aaatcgatct tgcgattgtg caggaagggt ttgcgaaccc
      241 cgaactgttc gagcaggcgc gcgccctggt gagagagact ccttccgaag ttgtaaccga
      301 tctctcaaca gatcaggatc cggcaatcca atggcgctat tatccgctcc tcagcggcca
      361 gagggcgatc gtcctgaggc accaaatcga atttttcagc gcgatgcaaa cactgacggc
      421 tctcgatatc aaagttgcgg ttcattccgc cccgatcgag atgctggcga tttatctgaa
      481 gctgtacgcg gaggcaatcg aggaaaaacc acattgcttc gtttttttct atgaccggtt
      541 caccgttgtc ataccggcga accaaggggt tctcgatgtc aaggtactac cccatcgcca
      601 gcaagatgtt cctccggcat ttggagacga tcttttctcc ctcctggaaa gattcggttt
      661 ggtcgattcc tgtgtgttgc tgcttgttca gtgcggcacc caggatccca cgctgctatt
      721 ccgcgaatta gacacttacg cgcgaagaaa caaaaaaaac gcgaatggga tcgaaattca
      781 gattcccgac aaagagatcg tttggaaagc cttcgatgag cacaccccgg ggcgggttaa
      841 agaagagata attcatcgtc cggagtttct tacggagtac aacgaaaggt ccggaaaaga
      901 ttttccgctc tcgcttggaa tcaaaagtga tgcgcaacgg ttcgggatct tgtgtcggga
      961 aaccttctgg ccggatgatc agaaatcccg ggatcagcgg cttccgcgga gcctcgctct
     1021 aacgattacc gcg
//
LOCUS       DATMKH010000314         1389 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3354308, whole genome shotgun sequence.
ACCESSION   DATMKH010000314 DATMKH010000000
VERSION     DATMKH010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3354308"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /gene="mreD"
                     /locus_tag="VNV63_03815"
     CDS             complement(<1..262)
                     /gene="mreD"
                     /locus_tag="VNV63_03815"
                     /inference="COORDINATES: protein motif:HMM:NF016019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HXC61780.1"
                     /translation="MKLVWYLLILILFVPVQSILMASISLNGVQPDLGLILLYFIGLV
                     YGEIDGVLLGLIIGFLMDLFSGGPLGPNLVSKTLLGWVSGMAG"
     gene            complement(280..1098)
                     /gene="mreC"
                     /locus_tag="VNV63_03820"
     CDS             complement(280..1098)
                     /gene="mreC"
                     /locus_tag="VNV63_03820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HXC61781.1"
                     /translation="MVRSLVNYRRLIVLILFTTLVIFLLSPELQHRPLLIIERPAGYL
                     ILFFQRGLGAVVQGISHSWSSYIALSGLRDETQRMKTEIEQLKTDNAQLQEMAIANDR
                     LTGLLEFNQRSPFRMTAAEVIGRDLSNWYRTIMINKGEKDGVSQDMGVVTPAGVVGRI
                     VKTSAINAHVLLLVDPNSAVAALIRRTREEGIVAGTERGLAQMKYLPAMADVKVGDLV
                     ITSGLAGGFPKGLPIGRVSLVEKKETDLTQHVEIIPDVDFGRLEEVLIITSIPF"
     gene            complement(1177..>1389)
                     /locus_tag="VNV63_03825"
     CDS             complement(1177..>1389)
                     /locus_tag="VNV63_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HXC61782.1"
                     /translation="TPPELAGDIIDRGIVLTGGGSLLRGMDTRLREETGLPIITVDNP
                     LTTVVLGTGKVLDELDLLRKVTIMSQ"
BASE COUNT          360 a          411 c          305 g          313 t
ORIGIN      
        1 ttccagccat tccgctgacc catccgagca aggtcttcga tacaagattg ggccccaagg
       61 ggccacccga gaaaagatcc atgagaaaac cgataattaa tccgagcaga accccatcta
      121 tttccccata aaccagaccg ataaaataaa gcagaattag tccaagatcc ggttgtacac
      181 cgttgagact gatcgaggcc atcaaaatag actgcacagg aacaaagagg ataagaatca
      241 ataagtacca gaccagcttc atatcgtttc agcctctctc taaaaaggga tggaagtgat
      301 gatcagtacc tcttccaggc ggccaaagtc cacgtccgga ataatctcca catgttgggt
      361 gaggtctgtt tctttcttct ccaccaggct taccctccca atggggaggc ctttcggaaa
      421 accacctgcc agcccggatg ttattaccag atccccgact ttaacatccg ccatcgcagg
      481 aagatacttc atctgagcca ggcccctttc ggtcccggcc acgatccctt cctcccgggt
      541 ccgccggatc agggccgcaa cagcgctgtt cggatctaca agcaaaagga catgggcatt
      601 tatggccgag gtcttcacaa tgcgacccac cacaccggct ggggtcacga cacccatatc
      661 ttggcttaca ccatccttct cacctttatt aatcatgatg gtccgatacc agttgctgag
      721 atcccgcccg ataacctcag ctgctgtcat tcgaaagggg cttctctggt taaactccag
      781 cagtcccgtc agccgatcat tggcgattgc catttcctgg agctgagcat tgtcagtctt
      841 gagctgctcg atctcggtct tcatccgctg agtctcatca cgtagaccac tcaaggcgat
      901 gtagctcgac caactgtgcg agatcccctg tacgaccgca cccaatccac gttggaaaaa
      961 cagaatcaaa tatcctgccg gccgctctat gatcagcaaa ggacgatgct gcaattcggg
     1021 agagagcaaa aagatgacca aggtggtaaa gaggatcaaa acgatcagcc gtctataatt
     1081 tacgagagaa cgaaccatga tcttcgcctg cctttatccc agtagggacg tattgccata
     1141 cgcccttact ttatccgccc gcacctaacc tccttttcac tgggacatga ttgtcacttt
     1201 ccttaagaga tccaattcgt ccaaaacctt gccggtgcct aaaaccaccg tggtgagagg
     1261 attgtccacc gtaataatcg gcagcccggt ttcctctcga aggcgcgtgt ccattcctct
     1321 caggagagag cccccaccgg ttaagacaat tcctcgatca atgatatctc cagccagctc
     1381 cggcggtgt
//
LOCUS       DATMKH010000315         1281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1431052, whole genome shotgun sequence.
ACCESSION   DATMKH010000315 DATMKH010000000
VERSION     DATMKH010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1431052"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(308..1102)
                     /locus_tag="VNV63_03830"
     CDS             complement(308..1102)
                     /locus_tag="VNV63_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61783.1"
                     /translation="MKTRILSVTLLLFLPLAVAAQTADEIVKKALDARGGVDRLKAIQ
                     TERVTGRITFSRDVEGTFVLELKRPLKMHLEISIDGQKIIRVYDGKSSGWTINPLAET
                     KDVQPLSSEDLKHISDESDFDGPLVDYKAKGNQIELAGKENLDDKPVYRLKVTNKNGD
                     IRFYFFDASSFLLLKWEGIRKTGDQELPWESFFSDFHEVQGLKYPFRIDQGSPGTEIK
                     QTLTAEKFEIDPQIDDSRFAKPSLPDAPADPAPSAPPAPPSPPLSN"
BASE COUNT          297 a          293 c          399 g          292 t
ORIGIN      
        1 agggagagat tgcacaggat gaagacggag ccgtgggcag attcgatgcg gcgagaatga
       61 agacaattgg cacagaggcc agcatccagg gagtcatgcg tctgttgccc tacatcagac
      121 atgaaagtaa ggatagcgca gtgcgtgggg gcggcgattg ttagaatgaa atgcagctcg
      181 gacttgtctt aaaaggattt gcggatgcac tccaaaccac tcctcagaac caacaagaaa
      241 agtcaaaaca cgaatgacta gcagtcaact tgcctggaag ggagaggcat cgctccgcgt
      301 gcgggagcta gttcgagagc ggaggactgg gtggcgccgg gggtgcgctt ggcgctgggt
      361 cggcaggggc gtctggcaga gagggtttgg cgaagcgaga atcatcgatc tgcggatcga
      421 tttcaaattt ttcggcggtg agagtctgct tgatttcggt gcccggactt ccctggtcga
      481 tccggaatgg atatttcagg ccctggacct cgtggaagtc ggaaaagaag gactcccagg
      541 gaagctcctg gtctcccgtc ttgcggatgc cttcccactt caaaagcaaa aaagaggagg
      601 catcaaaaaa gtaaaagcgg atatccccat tcttattcgt gactttcagg cgatagacgg
      661 gcttgtcgtc caggttttct ttgccggcta gttcgatctg gttgcccttg gccttgtaat
      721 ccaccagcgg gccgtcaaag tcggattcat cggagatatg tttgaggtcc tcggaagata
      781 gcggctgaac gtctttggtt tccgcgagag gattgatcgt ccagccggaa gacttgccat
      841 cgtaaacacg gataattttt tgcccatcga tggagatctc gaggtgcatc ttcaggggac
      901 gcttgagctc aagcacaaag gtgccttcca catctcgcga gaaggtgatt cggccggtta
      961 cgcgctcggt ctggatggct ttgagcctgt cgactccccc gcgagcgtcg agcgctttct
     1021 tgacgatttc atcggcggtc tgagccgcca cggccagcgg caagaacaag agtagagtga
     1081 cggagaggat tctggttttc atttgtgtct cgctggcgac gtgtcaaaga ctggggggcg
     1141 ccagattcat ccttggagta gaagcgtttt gggccaaatg cgttgccaga cggagcgggc
     1201 gcatcaagta tatgtgtgtt gtaaagttag cgaaacaatt ttccattaga ctccgttgaa
     1261 tgtagattta gagcggcacg g
//
LOCUS       DATMKH010000316         3565 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3983327, whole genome shotgun sequence.
ACCESSION   DATMKH010000316 DATMKH010000000
VERSION     DATMKH010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3565
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3983327"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="VNV63_03835"
     CDS             complement(<1..670)
                     /locus_tag="VNV63_03835"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXC61784.1"
                     /translation="MRFLIVLFVALGLVLAYLAYQNPGTVTVFLSQDQSFEVSVIALA
                     LLSMAFGGLVVIFVIGVRGTRNALLNWQRSRNRKREEKILNLYNEGMNAFLTKRYPEA
                     VMLFEKILLINSDHIPTLLRLGNLQRIQRNYGEAIRLHRKGRGLDEQNVELLLALARD
                     LEEAKRYEEAVQALRELVRLDESNLTALLKLRDLLIRLERWEEAHDLQEGILRLTQKE
                     SETKE"
     gene            complement(674..1150)
                     /gene="rsfS"
                     /locus_tag="VNV63_03840"
     CDS             complement(674..1150)
                     /gene="rsfS"
                     /locus_tag="VNV63_03840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HXC61785.1"
                     /translation="MLYNGPQFVPIEQGGTTVESSKKALTSVKAAWEKKAQDIVLLDL
                     RGLSSLADYFLLCSGESERQVRAIAQGIDASLTKLGVEPFSVEGMETSAWVLMDYNEL
                     IVHIFRSESRPFYALDRLWGDAPRMEFPQDRPLPVSALRFKNRSAEGTTGIRLGKG"
     gene            complement(1117..1779)
                     /gene="nadD"
                     /locus_tag="VNV63_03845"
     CDS             complement(1117..1779)
                     /gene="nadD"
                     /locus_tag="VNV63_03845"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HXC61786.1"
                     /translation="MKIGLLGGTFNPIHNCHLSIAEETRKRLSLDRVIFIPSHIPPHK
                     IDQEVIAARHRYEMVRLAIQPYPAFEVSDLEIKRPGPSYSVDTVQSLRGLLGVKTEIF
                     FILGLDAFLSIGSWKSTGTLLTLCAFVVISRPGSRCSDLARLSWCQHLDRKMLSSLDR
                     GDLDQGVGTLSSGTAIHLLRLPPCEISSTGIVERLKAKKDVKNLLPASVESYIMAHSL
                     YR"
     gene            complement(1811..3085)
                     /locus_tag="VNV63_03850"
     CDS             complement(1811..3085)
                     /locus_tag="VNV63_03850"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HXC61787.1"
                     /translation="MNIKNYVIKKAQEAKKASKQLALLSTEVKNKALALMAEALLRQQ
                     DKLIKANAKDLAEAKRRKLSSAMVDRLTLTPKRIVDMAAGLREVAALPDPVGEIVKNW
                     TRPNGMRIERMRVPIGVIGIIYESRPNVTADTASLCLKSGNAVILRGGSEAIHSNSAI
                     AKLLSQAAQSAAIPAGAITFIDLTDRKAVMELLKLDQYIDLIIPRGGESLIKTVLKNS
                     KIPVIKHDKGVCHTYVDESADLTMAEAICFNAKVQRPATCNAMETLLVHEKIAPDFLP
                     KMINHFQEAGVEIRGSMETRAILSRSGLVKGIKAATEKDWGAEYLDKTLAVKVVKDMN
                     EAIAHIVRYGSQHSDAIVTENRDRAKRFLREVDSCAVFVNASTRLNDGYQFGLGAEIG
                     ISTTKIHARGPMGLEELTSTKFVVQGSGQLRE"
     gene            complement(3188..>3565)
                     /locus_tag="VNV63_03855"
     CDS             complement(3188..>3565)
                     /locus_tag="VNV63_03855"
                     /inference="COORDINATES: protein motif:HMM:NF013626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PUA domain-containing protein"
                     /protein_id="HXC61788.1"
                     /translation="EALGGAEMGTLFFPKDQRRNSRQHWIAYTLKSKGRLHLDQGAVE
                     ALTKRGKSLLPSGMREVSGRFESGDAVSCLDPAGMEFAKGLVNYSSAELAKIKGAKST
                     EIVKILGYKDYDEVIHRDNLVIL"
BASE COUNT          795 a         1027 c          909 g          834 t
ORIGIN      
        1 cctctttggt ttcagactct ttctgcgtaa gccggaggat tccttcctga agatcatggg
       61 cctcttccca gcgctctaac cgaattaaaa ggtccctcag cttgagcagg gccgtgagat
      121 tagactcatc caagcgaacc aattcacgca gggcctgtac cgcttcctcg taacgcttgg
      181 cttcctccaa gtcccgggcc aatgccaaca ggagctccac attttgttca tcgagacccc
      241 ggcctttccg atggaggcgg atcgcctcgc cgtagttgcg ctgaatccgt tgaaggttgc
      301 cgagacgaag caaggtcgga atgtgatccg aattaatcag cagaatcttc tcaaatagca
      361 tgaccgcctc cggataccgt ttggtcaaga aggcgttcat cccctcatta taaaggttaa
      421 gaatcttctc ttcacgcttt cgatttcggc tccgctgcca attcagcaaa gcgtttcgcg
      481 tcccccgcac cccgatgaca aagatcacca ccagccctcc aaaagccatc gagagaaggg
      541 ccaaggcgat aaccgaaact tcaaacgact gatcctggga aaggaaaacc gttacagtgc
      601 cggggttctg ataggctaga taggccagga ctaaaccgag tgcgacaaac aatacaatca
      661 gaaaacgcat aagttaacct ttgccgagcc gtatgccggt ggttccttct gctgaacgat
      721 ttttaaaacg gagagcggaa acaggcaggg gacgatcttg agggaactcc attcgcggtg
      781 catctcccca caagcgatcc agcgcgtaga agggtctgct ctcggacctg aaaatgtgga
      841 cgatgagttc gttgtaatcc atcaacaccc aggctgatgt ctccatccct tccacgctga
      901 acggttcaac ccccaatttt gtaagcgagg cgtctatgcc ctgcgcaatt gcccggacct
      961 gcctttccga ttcgcccgag cagagtaaaa agtagtccgc taggctggac agtccgcgca
     1021 gatcaagcag cacaatgtcc tgagcttttt tctcccaggc agctttgaca gaggtcagag
     1081 cctttttgct ggattcgacg gtggttcctc cctgctctat cggtacaaac tgtgggccat
     1141 tatataggat tcgaccgagg caggcaagag atttttaacg tccttttttg cctttaatct
     1201 ttctacaatc ccggttgatg agatctcgca gggagggagg cgaagaagat gaatggctgt
     1261 gccggaggaa agcgtgccga ccccctggtc cagatcgccc cgatcgaggc tgcttagcat
     1321 ctttcgatcc agatgctgac accaggagag ccgggccaag tccgaacaac ggctgccggg
     1381 tctcgaaatc accacgaagg cgcaaagggt aaggagcgta ccggttgact tccaggaacc
     1441 gattgaaaga aaggcgtcca ggccgagaat aaagaagatc tcggttttta cacccaagag
     1501 gccgcgaagg gactgaaccg tgtccaccga ataagaagga ccgggccgtt tgatctccag
     1561 gtctgacacc tcaaaggcag gatacggttg gatcgccaga cggaccatct cgtaccgatg
     1621 gcgcgccgcg attacttctt gatcaatctt gtggggagga atatgtgaag ggatgaagat
     1681 gacgcgatcc agggacagcc gctttcgggt ctcttccgca atcgaaaggt ggcagttgtg
     1741 gatcggattg aatgtccccc ccaagagacc gattttcatg aatgggccat attttgatgc
     1801 atttgaccgg ttactccctc aactgaccgc tgccttgaac gacaaacttg gtcgaggtga
     1861 gttcctccag ccccatcggc ccgcgggcat ggatcttagt agtgctgatc ccgatctcgg
     1921 ccccgagccc gaactgatag ccatcgttca ggcgcgtcga ggcatttaca aacacggcgc
     1981 aggagtccac ttcccgaaga aaccgtttgg cgcggtcgcg attttccgtc acgatcgcat
     2041 ccgaatgctg agagccgtac cgaacgatat gggcgatcgc ttcattcata tccttgacga
     2101 cctttaccgc caaggtcttg tcgagatatt cggcccccca gtctttttcg gtcgccgcct
     2161 tgattccctt aacaagcccg ctccggctca gaatcgcccg cgtctccata gatccacgga
     2221 tctccacccc ggcttcttgg aagtgattta tcatcttggg cagaaagtcc ggcgcaatct
     2281 tctcgtgcac cagtagcgtc tccatcgcgt tgcaggtggc cggtcgctgg accttggcat
     2341 tgaaacagat cgcctcggcc attgttagat ccgccgattc atcgacatac gtgtggcaga
     2401 cgcctttatc atgcttgatc accgggatct tggagttttt caacaccgtt ttgatcaggc
     2461 tctcgccgcc gcgcggaata atgaggtcaa tatactggtc cagcttcaaa agctccatca
     2521 cggcctttcg gtccgtcaag tcaatgaagg taatcgctcc ggcaggaatt gcggccgact
     2581 gggcagcttg cgagagcaac ttggcaatcg cgctgttaga atggattgcc tcggagcctc
     2641 cgcgcagaat gacggcattg ccggatttta aacacagact tgcggtatcg gccgtgacgt
     2701 tgggacgaga ctcgtagatg attccgatca caccgatcgg cacgcgcatc cgctcgatcc
     2761 tcatcccgtt cggccgcgtc cagtttttga cgatctcgcc caccgggtcc ggcaaggccg
     2821 ctacttccct taagcccgcg gccatatcga caatgcgttt gggagtgagc gtcagacgat
     2881 ccaccatcgc gctggaaagt ttccgccgtt ttgcctcggc aaggtccttt gcattcgcct
     2941 tgattaactt gtcctgctgc ctcaagagcg cctcggccat aagggccagt gccttatttt
     3001 ttacctcggt ggaaagaaga gcaagctgtt tagaagcttt ctttgcctct tgggcttttt
     3061 tgatcacgta gttttttata ttcataatat ctctcattaa atcggtgggc cgctgcggca
     3121 agaaggaaca aggtcctcac atgccccccg cacccccgcg ctcgcgtcgg caacgctgga
     3181 cgctcactta caatatcacc aaattgtcac ggtggatcac ttcatcgtaa tccttgtagc
     3241 ctaagatttt gacgatctcg gttgacttgg cgcctttgat ctttgcgagt tcagccgagc
     3301 tatagttcac caatcccttg gcaaactcca ttcccgcggg atcgagacag ctcacggcat
     3361 cgcctgactc aaaacgtccg ctcacctcac gcatcccgga cggcagcagg ctcttgcctc
     3421 tcttcgtaag cgcctcgacc gcaccctgat ctagatgcag ccggcctttg ctttttaacg
     3481 tatatgcgat ccagtgctga cggctgttgc gcctttggtc cttgggaaaa aacagcgtcc
     3541 ccatttcagc accacccagc gcttc
//
LOCUS       DATMKH010000317         2119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1431291, whole genome shotgun sequence.
ACCESSION   DATMKH010000317 DATMKH010000000
VERSION     DATMKH010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1431291"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VNV63_03860"
     CDS             <1..366
                     /locus_tag="VNV63_03860"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HXC61789.1"
                     /translation="LLPVLDNLARAIGHAKESRNLDKLVEGLELTQKEFVSVLNKFGV
                     TAIESLGQPFDPVHHQAMLQVDTDDQEENTVVEEFAKGYLLHDRVLRPALVSVAKNAK
                     KAASPLHGQGTEKTEEDHN"
     gene            412..>2119
                     /gene="dnaK"
                     /locus_tag="VNV63_03865"
     CDS             412..>2119
                     /gene="dnaK"
                     /locus_tag="VNV63_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HXC61790.1"
                     /translation="MTKVIGIDLGTTNSCVAIMSGGDPVVIANAEGSRITPSVVAITD
                     KGERLVGQIAKRQAITNPENTIFSIKRLIGRKFKAKEVQEALKRLPYKVVEAPNGDAH
                     VEIRGKVYSPAEISAMILQKMKQTAEDYLGQPVTEAVITVPAYFDDSQRQATKDAGAI
                     AGLTVLRIINEPTAASLAYGLDRKKDERIAVYDLGGGTFDVSILEIGEGVFEVKATNG
                     DTYLGGDDFDQRVMDWLVEEFKKDQGIDLRKDKMALQRLKEAAEKAKIELSSSLETEI
                     NLPFITADAAGPKHLVIKLTRSKLEQLADDLVQRTIEPCRKAVTDAGISAKEINEVVL
                     VGGMTRMPKVQQLVKEFFGKEPHKGVNPDEVVAVGAAIQGAVLKGEVKDVLLLDVTPL
                     SLGIETLGGVFTKLIERNTTVPTRKSQTFSTAADNQTAVSVRVFQGEREMAADNKLLG
                     QFELVGIPPAPRGIPQVEVTFDIDANGIVHVSAKDLATGKEQSIRITASSGLSKEEIE
                     KMVRDATAHSEEDKKKKEMVEIRNEADTLCYSVEKSLTDFGDRISEAERNTIKDKITA
                     TRK"
BASE COUNT          578 a          524 c          606 g          411 t
ORIGIN      
        1 ttgctgccgg ttctggataa tctagctcgg gccatcggcc atgcaaagga aagcaggaat
       61 ttagacaagc tggttgaagg tcttgaactc acacagaaag aatttgtttc tgtcctaaac
      121 aagtttggag tcaccgccat tgagagtctc gggcaaccgt ttgatcctgt tcatcaccag
      181 gcgatgctcc aggttgatac agacgatcag gaggagaaca ctgtggtcga agagttcgca
      241 aaaggttacc tcctccatga ccgtgtctta cggcctgccc ttgtcagcgt agcgaaaaat
      301 gccaagaagg cggcttcacc gcttcatgga caagggaccg agaagacaga agaagatcat
      361 aactgaaaat gaacatctaa agacagcagg attttaagga ggaataatac catgacaaag
      421 gtaatcggta ttgatctagg gacaaccaac tcctgtgttg ccattatgtc gggaggcgat
      481 ccggtggtaa tcgccaatgc ggagggcagc cggatcactc cttccgtcgt tgcgatcacg
      541 gataaagggg aacggttggt ggggcagatc gcgaaacgcc aggcgattac aaatcccgag
      601 aacacgatct tttcgatcaa gcgattaatc ggtcgaaaat ttaaagccaa agaagtacag
      661 gaggccttaa agcgtcttcc ttataaagtc gtagaggccc cgaatggaga cgcccatgtg
      721 gagatccgcg ggaaggtgta tagcccggcc gaaatctcgg caatgatcct acaaaaaatg
      781 aagcagactg ccgaggacta cttgggtcag ccggtcacgg aagccgtgat cacagtcccg
      841 gcttacttcg atgacagcca acgccaggcg accaaagacg ccggggcgat cgcgggattg
      901 actgtcctcc ggattatcaa tgagccaacc gccgcttcgt tggcctacgg tctcgataga
      961 aagaaagatg agcgaatcgc agtgtatgac ttgggtggcg gaaccttcga cgtctccatt
     1021 ctcgaaatcg gagaaggggt ttttgaggtc aaggccacca atggagacac ctatttgggt
     1081 ggggatgatt ttgatcagcg cgtcatggac tggctggtgg aagagttcaa aaaggaccag
     1141 ggcatcgatc ttcgaaagga caagatggct ttacagcggc tcaaagaagc ggctgagaag
     1201 gccaagatcg agctgtcctc ttctctggaa accgagatca acctgccatt tatcacggcc
     1261 gatgcagcag gccccaaaca tctggtgatc aagctcaccc gatcgaagct ggaacagctc
     1321 gcggatgatc ttgtccagcg aacgatagaa ccttgccgga aggccgtgac ggatgccggt
     1381 atctccgcaa aagaaattaa tgaggtggtg ttggtaggcg gcatgacacg tatgccgaaa
     1441 gtccaacaac tcgtcaagga gttcttcggc aaggagcctc acaaaggggt caacccggat
     1501 gaagtggtgg ccgtgggcgc tgcgatccaa ggggctgtac tcaaaggcga ggtgaaagat
     1561 gtcctgcttt tggatgtcac ccctctctcg ttgggcattg agaccttagg cggtgttttc
     1621 accaaactga tcgaacggaa caccacggtc ccaacgcgta agagccaaac cttctccacc
     1681 gccgcggaca accagacagc agtctcggtc cgggtcttcc agggagagcg tgaaatggcc
     1741 gccgacaaca aactgctggg acagttcgaa ttagtgggaa tcccaccggc cccccgcggc
     1801 atcccccagg tcgaggtgac ctttgatatt gatgccaatg ggatcgttca tgtctcggca
     1861 aaagatctcg caacgggtaa ggagcagtcc atccggatca cagccagcag cggactgtcc
     1921 aaggaagaga tcgagaagat ggtcagagat gccaccgccc attcagaaga ggataagaaa
     1981 aagaaggaga tggtcgagat acggaacgag gccgacactc tctgctacag cgttgagaaa
     2041 tccctgaccg actttggcga tcggatctcg gaggccgagc ggaacaccat aaaagacaag
     2101 atcacggcca cgcgcaagg
//
LOCUS       DATMKH010000318         1090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1114078, whole genome shotgun sequence.
ACCESSION   DATMKH010000318 DATMKH010000000
VERSION     DATMKH010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1114078"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..127
                     /locus_tag="VNV63_03870"
     CDS             <1..127
                     /locus_tag="VNV63_03870"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HXC61791.1"
                     /translation="FEVSGQYLVPVLVDGDTVLDDEHDIIEHLETHYPHPKEKPA"
     gene            127..>1090
                     /locus_tag="VNV63_03875"
     CDS             127..>1090
                     /locus_tag="VNV63_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HXC61792.1"
                     /translation="MEKWKEIFRKSLVKPEDLAKHFDMDPDAIRETVANYPMRITPYF
                     LNLIKDKDDPIAKQVIPDPTEMQEPLTLCMDDPLSEEADSPVPNLVHRYPDRVLLMVT
                     TQCPIYCRFCTRKRLIGQPGYVTKQTIQQGIDYIAQHSEVRDVILSGGDPLLLSDDYL
                     EWVLKSLRAIPHLELIRIGSRVPGSLPQRITKKLCRMLKRYHPLYMNLHFNHPDEITP
                     ESKKACERLADAGIPLGSQTVLLKGVNDDPETMKTLMQKLLSIRVKPYYLYQADITKA
                     TDHFRTSVEAGLNIMRHLRGFTSGMAVPHYVIDAPGGGGKIAVLP"
BASE COUNT          270 a          311 c          277 g          232 t
ORIGIN      
        1 attcgaagtt tccggacagt acttagtgcc cgtcctcgta gacggagaca cagtcttgga
       61 cgacgagcac gatatcatcg agcatcttga gactcattat ccacatccca aggagaaacc
      121 ggcctaatgg aaaaatggaa agagatattt cgtaagagtc tggtcaaacc tgaagacctg
      181 gccaagcatt ttgacatgga ccccgatgcg atccgtgaaa cggtggccaa ctatcccatg
      241 cggatcacac cctattttct gaacctcatt aaagataagg atgacccgat cgcaaagcag
      301 gtgatcccgg atccgaccga gatgcaggaa cccttgactc tctgcatgga tgatccattg
      361 tccgaagagg cggatagccc ggtgcccaat ctcgtccacc gatatccgga tcgcgtcctg
      421 ctgatggtca ctacccagtg cccgatctac tgccgtttct gcacacgaaa gcgtttaatc
      481 ggccagccgg gctatgtgac caagcaaaca atacagcagg gcatcgatta catcgcacag
      541 cactctgaag tccgggacgt gattctctcc ggcggcgacc cgctcttatt atcggatgat
      601 tatctggaat gggttttgaa atcgcttcgc gccattcccc atctggagct gatccggatc
      661 gggagccgag tcccgggctc tctgccgcaa cgcatcacca aaaaactctg ccgtatgcta
      721 aaacgctatc acccgcttta catgaacctt cacttcaacc atccggacga gattacaccg
      781 gaaagcaaga aggcctgcga gcggctggcg gatgccggca ttcctctcgg cagccagacc
      841 gttttattga aaggggtgaa tgacgatccg gagacgatga agaccttgat gcagaagctt
      901 ctttcgatcc gtgtgaaacc gtattatctc taccaggccg atatcactaa agcgacggat
      961 cacttccgga ccagcgttga ggccgggctc aacatcatgc ggcacctgcg cggttttaca
     1021 agcggcatgg cggttccaca ctacgtgatc gatgcacccg gcggcggtgg caagatcgcg
     1081 gtgcttccac
//
LOCUS       DATMKH010000319         1189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3034329, whole genome shotgun sequence.
ACCESSION   DATMKH010000319 DATMKH010000000
VERSION     DATMKH010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3034329"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="VNV63_03880"
     CDS             <1..503
                     /locus_tag="VNV63_03880"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HXC61793.1"
                     /translation="KLFHSKATCFMCHGATGKGDGVLAAALKDVWGHPIRPANFTLPA
                     GVKGGVELGHDGEHIFKTIMTGVGGSPMPAFQGTLKPDEVWDIVHYEQSLRVKAHGSE
                     LIAEGLKDQDLADALSGMWEELSVSAAQDRIEQALLEQELAELKSEKGRTIQPGLSEE
                     TPATAH"
     gene            complement(512..>1189)
                     /locus_tag="VNV63_03885"
     CDS             complement(512..>1189)
                     /locus_tag="VNV63_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61794.1"
                     /translation="QKIKETYPRIRYFDSRLHRLPQDFTAWLKESPETETAESPAAPP
                     NVIPQDHPQPLVVTPAQPPAVVKTPPPSIVSPDDKTRLILSNRQGFNAGALTGANEEV
                     RRLFAEAKQDYQLKNYSLVEKKLRQIIELAPEEAIAYHLLGMLFIDRNDEDAAMKIFN
                     EGSGNLPQAPLLHYDLGFLYYKRGITSLAKVELGKALALRPSAPQAGRAQEILAELNQ
                     QPTQDNP"
BASE COUNT          262 a          286 c          369 g          272 t
ORIGIN      
        1 agaagctctt ccatagtaag gccacgtgct tcatgtgcca tggggcaact ggaaaaggag
       61 atggggtctt ggcggcagcc ctgaaggatg tctggggtca tccgatacgt cctgccaact
      121 tcacgctgcc ggccggcgtg aaaggagggg ttgaactggg ccatgacggg gagcacatct
      181 ttaagacgat catgaccggt gtgggtgggt ccccaatgcc tgcctttcag ggaacgttaa
      241 aacctgacga ggtctgggac attgttcatt acgagcagtc cttacgcgtg aaggcccatg
      301 ggtccgagtt gattgcagag ggtttgaaag atcaggatct agctgatgcg ctctccggga
      361 tgtgggagga gctttcggtt tccgctgctc aagacaggat cgagcaggca ttgctggagc
      421 aagaactggc ggagctgaag tcggaaaagg gccggacgat tcagcccgga ctgtcagagg
      481 agacgcccgc gactgcacat taaaaaaacc ctcatggatt atcctgcgtc ggttgctgat
      541 tcaactcggc cagtatctct tgggcgcgcc ccgcttgagg ggctgacggc cttagtgcca
      601 aagccttgcc caactccacc ttcgccagtg aggtgatgcc tcgtttataa tagagaaagc
      661 ccagatcgta atgaagcagc ggggcttgag gcaggttgcc ggatccttca ttgaaaatct
      721 tcatggcagc atcttcatca ttgcggtcta taaaaagcat tccgagaaga tgataagcga
      781 tcgcctcctc gggggccagc tcgatgatct gacggagctt cttctccaca agagaataat
      841 tcttaagctg ataatcctgc ttggcttctg cgaagagccg gcggacctct tcgtttgcgc
      901 ctgtcagggc gccggcattg aatccctgcc ggttcgatag gatcaaacgg gttttatcat
      961 ccggcgatac aatcgaagga ggaggcgttt tgacgacggc gggcggctgg gctggtgtta
     1021 caacaagcgg ttggggatgg tcctgaggta tcacattcgg tggggcagcg ggagactcgg
     1081 cagtttctgt ttcaggtgac tccttcaacc aggccgtgaa atcttgagga agccgatgga
     1141 gcctggaatc gaagtagcgg attctgggat aggtctcctt gatcttttg
//
LOCUS       DATMKH010000320         4480 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1748245, whole genome shotgun sequence.
ACCESSION   DATMKH010000320 DATMKH010000000
VERSION     DATMKH010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4480
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1748245"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="VNV63_03890"
     CDS             complement(<1..371)
                     /locus_tag="VNV63_03890"
                     /inference="COORDINATES: protein motif:HMM:NF016553.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61795.1"
                     /translation="MLLTHLVERVIQVRATTKKTEKISLLADFLRQTHGKETELAALY
                     LSGSLPQGKIGIGWRMIEDATPEALSSGERPTLLEIDRVFESIALDQGPGSLERKIGV
                     LRRLLERANPDERRFMAQLIIG"
     gene            complement(478..1599)
                     /gene="alr"
                     /locus_tag="VNV63_03895"
     CDS             complement(478..1599)
                     /gene="alr"
                     /locus_tag="VNV63_03895"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HXC61796.1"
                     /translation="MTGFHSTMIEVDLVVLVHNLHEIRKKVGAQSKIMTVVKANAYGH
                     GAVPVASALARAGADWLGVASVQEGIELRQAGLSLPILVMGGVLEPELEGLAVHQLTP
                     VVYHPEMVPMLEDTAARSGKPIKIHVKVDTGMGRLGLSPDIAALFIQKIVQRGRLTVE
                     GLMTHFAEADMKDQIFVQEQLQKFRRVLKDLHDLGIEIPLSHLANSAAILHHPTAYFQ
                     MVRPGIALYGYTPGTGISNRPDLRPCLQLVTRIVQVKRVPKGTGISYGRSFITKRESL
                     IASLPIGYADGYSRRLSNKGQVIVREQRAPIIGRVCMDMTMVDVTEVPDVRIGDPIVL
                     LGQQGNQAITADEIARWTDTIPYEVLSILNPRIARKYTG"
     gene            complement(1700..2272)
                     /locus_tag="VNV63_03900"
     CDS             complement(1700..2272)
                     /locus_tag="VNV63_03900"
                     /inference="COORDINATES: protein motif:HMM:NF015377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HXC61797.1"
                     /translation="MKRSLLSLCLIGLLFLLMGCGSPAIGPNEALLEKPKLGSHLIQN
                     VPFFPQEEYYCGPSALASLLTYYGYPTTQDAIAREIYLPRHFGTLSMDLLLYSKAKGL
                     DTKVVEGKLDLLKSEIQQKHPIVVFLNLGLKIFPKGHFITVIGYDDMNQIIIAHSGRE
                     PEQRIPYGRFLRAWEKTGFWTLLVNQKQPA"
     gene            complement(2284..2700)
                     /locus_tag="VNV63_03905"
     CDS             complement(2284..2700)
                     /locus_tag="VNV63_03905"
                     /inference="COORDINATES: protein motif:HMM:NF033919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2779 family protein"
                     /protein_id="HXC61798.1"
                     /translation="MRRNLKELLKQSLIGYMIVAVAVLTVVPADVQAMFLPSGVDLTQ
                     GDSSLDRQQNLERLQRLLESRLISQRLSDLGFSQGEVSSRLNRLSDEQVHFFSSHLES
                     LQTGGDGLGIIIALLVIAVLVVVLLEVTGHRVLITK"
     gene            complement(2727..3287)
                     /gene="frr"
                     /locus_tag="VNV63_03910"
     CDS             complement(2727..3287)
                     /gene="frr"
                     /locus_tag="VNV63_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HXC61799.1"
                     /translation="MPPAELKKKMIEKMEGAQDHLRSELSGIRTGRASLILLDGIKVD
                     YYGTPTPVRQIASLATPEPRLITIQPWEPNLIGEIERAIQASDLGVTPTNDGKIIRLA
                     VPPLSEERRKELVKLAKKLGEETKVIIRNIRRDTNEELKKLHKDTKVTEDELKKGQDE
                     VQKLTDQGVQRVDEIIKKKESEILEV"
     gene            complement(3300..4016)
                     /gene="pyrH"
                     /locus_tag="VNV63_03915"
     CDS             complement(3300..4016)
                     /gene="pyrH"
                     /locus_tag="VNV63_03915"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HXC61800.1"
                     /translation="MNPPKFQRILLKISGEVLAGDQGAGIDPYVIDTIAEEIKEVHGL
                     GVEIAIVIGGGNIFRGIAASATGMERASADYMGMLATVLNALALQNALERKNVFTRVQ
                     SAIEMRALAESYIRRRAIRHLEKKRVVIFAAGTGNPYFSTDTAAALRAMEIGAQVILK
                     GTKVDGVFDADPLKVPNAIKFDQLSFLDVIQRDLKVMDTTAVSLCMDNNLPIIVFNIK
                     LKGNIKDVVLGEKIGTWVRK"
     gene            complement(4026..>4480)
                     /gene="tsf"
                     /locus_tag="VNV63_03920"
     CDS             complement(4026..>4480)
                     /gene="tsf"
                     /locus_tag="VNV63_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006522456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HXC61801.1"
                     /translation="RSTREGLIGTYIHAGEKLGVLIEVNCETDFVARTEEFRGLVRDL
                     AMQIAGAHPSPRYIQSQDVPKEIVEKERQIYLAQAKESGKPEKAWDKIVEGKLAKFYS
                     EACLLEQTFIKDPNITINQLLVQKISQLGENIAIRRFTRYRVGEEQEA"
BASE COUNT          942 a         1214 c         1193 g         1131 t
ORIGIN      
        1 cctatgataa gttgggccat gaagcggcgt tcatcaggat tcgcccgttc aagcaggcga
       61 cgcagcacgc cgatctttcg ttcgagcgag ccgggccctt gatccaaagc gatgctttca
      121 aacacccggt ctatttctag aagcgtgggc ctctcgcccg atgaaagggc ttcgggtgtt
      181 gcatcttcaa tcattcgcca accgatgccg atctttcctt gcggaagtga gccggagaga
      241 tagagcgcgg ccagctcggt ttccttaccg tgcgtctgcc ggaggaagtc cgccaggaga
      301 gagatctttt ctgtcttttt cgtggtggcc cgaacctgga tgacccgttc cactaaatgg
      361 gtaagcagca tatcgctatt atactaatac cgaccccgtg gatgaaagat agcgcggcgg
      421 acggcttcag catacgatgg gcatcagata ttatgagata tggcggggca ctaaagacta
      481 tcctgtatat tttctggcga ttcgggggtt cagaatagat aacacctcgt aggggatcgt
      541 gtctgtccag cgcgcaatct catcggctgt aatggcctgg ttcccttgct gccccaggag
      601 tacaatcgga tctccaatgc ggacatccgg gacttcggtg acatccacca ttgtcatgtc
      661 catacagact ctgccgatga tcggggcccg ttgttcacgg acaatcactt gccccttgtt
      721 agagagccgc cggctgtagc cgtccgcgta tccgatcggg aggcttgcga tgaggctctc
      781 ccgtttcgtg atgaaagacc ggccatagct gatcccggtg ccttttggaa cgcgctttac
      841 ttgaacgatg cgagttacaa gctgcaggca cggtcttaga tccggccggt ttgagatgcc
      901 tgttccgggt gtatagccgt acagggcgat cccgggacgg accatttgaa aataggcggt
      961 cggatgatgg aggatcgctg cgctgttggc cagatgagag agagggatct cgattcccag
     1021 atcatgaaga tcctttaaga cccgacgaaa tttttggagc tgctcctgca cgaatatctg
     1081 gtccttcatg tccgcttctg caaaatgagt catcagccct tctacggtca gccgaccccg
     1141 ctgaacgatc ttttgaatga acaaggccgc gatgtccgga gaaagaccga gccggcccat
     1201 tccggtatcc accttcacat ggattttaat gggcttgcct gaccgagccg cggtatcttc
     1261 caacatggga accatctctg gatggtacac aacaggagtg agctgatgta ctgccaaacc
     1321 ctccaactcc ggttcgagga ccccgcccat cacgaggatc ggaagggaga gacccgcctg
     1381 gcgcagttcg attccttcct gcactgaggc tactcccaac cagtccgcac cggcgcgagc
     1441 caaggccgat gcgaccggca ctgccccatg tccgtacgca tttgccttga ccacagtcat
     1501 gatcttagat tgggcgccca ccttttttcg gatttcatgg agattgtgga ccaaaacgac
     1561 caggtccacc tcgatcatcg ttgagtgaaa cccggtcatt tatacctccc ggaggggccc
     1621 ttgtatttcc cgaaggggcc cgtatatctc ccgcaggggg atgtgagttc gatcctcttc
     1681 gacggcattt ttttatgtgc tacgcgggtt gtttttggtt aacgagcagc gtccagaagc
     1741 ctgttttttc ccaagcccgg agaaaccggc catacggaat gcgctgctcc ggttctcgac
     1801 cggagtgggc gatgatgatc tgattcatat catcgtaacc gatgacagtg ataaagtggc
     1861 cttttgggaa gatcttcagg ccgagattca gaaagaccac gattgggtgc ttctgctgga
     1921 tctcggattt caggaggtct agttttccct ccacgacttt agtgtctagg ccttttgctt
     1981 ttgagtagag gagaagatcc atcgaaagtg ttccaaagtg gcggggcaga tagatctctc
     2041 gggcgatcgc atcctgtgtt gtgggataac cgtaataggt gagaaggctg gccagggcgg
     2101 acggaccgca gtaatattct tcttggggaa agaacgggac attttgaata agatggctgc
     2161 cgagcttcgg cttctcgagc agcgcctcat tgggaccgat ggcgggtgag ccgcatccca
     2221 ttaacaaaaa cagaagcccg atgaggcata gggatagtag gcttcgtttc attgttgcag
     2281 aatttacttt gtgatgagga ccctgtgtcc ggtcacttcc agcaaaacca cgaccaggac
     2341 cgcgatgacc aacaaggcga tgatgattcc cagaccgtcc cctccggtct gaaggctttc
     2401 aagatggctt gaaaaaaaat gcacttgctc atcggacaac cggttgagcc gggacgagac
     2461 ttctccttga gagaaaccga gatcggaaag ccgctgagag atcagccggg actccaagag
     2521 cctctggagc ctctctagat tttgttgccg atccagggac gagtcacctt gagtaaggtc
     2581 aacaccggac ggcaaaaaca tggcctgaac atcggctgga actaccgtta gaacggcaac
     2641 agccacgatc atgtatccga tcagggactg ttttaatagc tctttaagat tccgtctcat
     2701 agaatctcct ctcgaatatc gccctgctaa acttccagga tttctgactc tttctttttg
     2761 attatttcgt caacccgctg gacgccttga tccgtgagtt tttggacctc gtcttggccc
     2821 ttttttagtt cgtcctctgt gacctttgta tccttgtgga gttttttaag ttcttcattc
     2881 gtgtcgcggc ggatgttgcg gatgatgacc ttcgtctcct cgccgagttt tttggcaagc
     2941 tttaccagtt ccttccgccg ctcttcactc aatgggggaa ccgcaagccg gatgatcttt
     3001 ccatcattcg tgggcgtaac tcccagatcg gaggcttgaa ttgcccgttc aatttcaccg
     3061 atcaaattgg gttcccaagg ctggattgtg atcaaccgcg gttcgggcgt ggccagagag
     3121 gcgatttgtc gtaccggggt gggcgttcca tagtagtcca ccttgatgcc atcgaggaga
     3181 ataagcgacg cgcgtccggt tcggattcct gacagctccg aacgaagatg gtcttgggcc
     3241 ccctccatct tctcgatcat cttcttttta agctcggcgg gcggcattga tttaaaactt
     3301 tattttctga cccatgtccc gattttttct cccaggacca cgtctttgat gtttcccttt
     3361 agtttgatgt tgaaaacaat gatcgggaga ttattgtcca tgcagagcga gacggcggtg
     3421 gtatccatca ccttcaagtc ccgttggatg acatccagga agctgagctg gtcaaatttt
     3481 atggcgttcg gcactttcaa tggatccgcg tcgaagactc catccacctt ggtgcctttt
     3541 aagatcacct gggccccgat ctccatggcg cggagcgccg ccgcggtgtc ggttgaaaaa
     3601 taggggttcc cggtccctgc ggcaaagatc acgacccttt ttttctccag atgccggatg
     3661 gctcgccgcc gaatatagct ctcggccagc gcccgcattt cgatcgcaga ctggacccgt
     3721 gtaaagacgt tttttcgctc gagggcgttc tgcaaggcca gggcattcag gaccgtggcc
     3781 agcatgccca tgtagtccgc cgatgcccgt tccattccag tcgcgctggc ggcgatgcct
     3841 cggaagatgt tgccgccgcc gatgacaatc gcgatctcga cacccagccc atggacttct
     3901 ttgatctctt ccgcaatcgt gtcgatgaca tagggatcaa tccccgcacc ttgatctcca
     3961 gccaaaacct caccgctgat ctttaaaagg atgcgctgga actttggcgg gttcatcccc
     4021 ttgacctagg cctcttgttc ttcacccact cggtagcggg taaatcttcg gatcgcaatg
     4081 ttctcaccca gctgggaaat cttctgaacc agaagttggt tgatcgtgat attcggatct
     4141 ttgataaacg tctgctccaa aagacaggcc tcggagtaga atttggccag ctttccttct
     4201 acgatcttgt cccacgcctt ctccggctta ccgctctctt tggcctgggc cagatagatc
     4261 tggcgttctt tttcgacgat ttctttcggg acatcctgcg attgaatata gcgcggggat
     4321 ggatgggccc cggcgatctg catcgccagg tctctgacca gtccccggaa ctcctcggtt
     4381 cgagccacaa aatcggtctc gcagttgacc tcgatcagaa ccccgagttt ttcaccggca
     4441 tggatgtaag ttccgatcag gccttctcga gtagagcgac
//
LOCUS       DATMKH010000321         1127 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4628756, whole genome shotgun sequence.
ACCESSION   DATMKH010000321 DATMKH010000000
VERSION     DATMKH010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4628756"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
BASE COUNT          233 a          316 c          266 g          312 t
ORIGIN      
        1 ctcgtgtctg ttgcgtgcac gcgagtttct cgggaatctc gatcctccgc agtatgtgcc
       61 cgatctcttg aagttcgggt tcccatcgct ttgatcggga aatcccgcaa agggcggagc
      121 caagattcac ttgacctcat gtaatggcta aaagggttat cattccacgt cctgtctgtg
      181 tttcgccgtt ggtctttggt tggcttttcg tggcgagcga ggaatggtcg ccgccttcga
      241 caacgtaaaa ttcacttgcg gcgcgcattt cttcgcgaac acgtgcgagg ttgtccaact
      301 ttgtgccgga tccatccctt tttgggatat ccggaaaaag aaagattcta ctgtaatttc
      361 ggattcgtca cccggcgtca gcctcgaagc gtgaaatgtt cggcgtggct tatggccata
      421 aaccgcatga acgcggggcc cttagagcga ccgtcggcga atctcgctcc tttatattgg
      481 aggttctcac aacgagtcct ctttgtgccg atgaccgtct ttccatcagc catattccct
      541 tttctcaggt ttctccctta ttctgccata agggtcgcgc gtggtgttcg acttccattg
      601 gaaagagcac cagtgtcctt cccgctctct tccccctgag gcgctcaatt ccggcaccca
      661 aaaagcctga gggcatccta cgtaaatggc tcccatttcg gcgaaagtcg tccagatcgc
      721 attcgacaaa aatccagctc catttcacac ctttagattc ggaaaaatta aaccgttcac
      781 cggtggctac cacgatggcc aactcgatct cttttactac cgagatcccg aacttcacga
      841 gagggaaccg ttccctttgt tgacgattct ttgaagcttt gaagcgactc acaccgtgcc
      901 ccaatttgca caagccctac ttccaaatgt tactgcggac ctcatgatgc gccggtaggt
      961 tcctcgatct tgggatcggg aaccgtatcg actcggacgt cgttttacca tgccaacaga
     1021 gggccctcat ttaaacatct ttttttggtt cttgtggatc ctgaagtaca aaagatgggt
     1081 tgagattgag aaggcctccc gcgctccatt atcccttttc cggcctt
//
LOCUS       DATMKH010000322         1267 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3986754, whole genome shotgun sequence.
ACCESSION   DATMKH010000322 DATMKH010000000
VERSION     DATMKH010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1267
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3986754"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="VNV63_03925"
     CDS             complement(<1..518)
                     /locus_tag="VNV63_03925"
                     /inference="COORDINATES: protein motif:HMM:NF016830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HXC61802.1"
                     /translation="MKPQISKMRNSTAEGKASRIIAPILSVLIFGVSTFDGSAGSTKE
                     ADFLTQVHSMTEENGVTFTGEYTGEIFGNLSGGIQQGASYEGLLKLTLRLDLQKIVHW
                     DGASIYASALYPHGNGLSRRYTGDFNILSSIDAYDSVRLFELWFEQKFFGGKASIRIG
                     QMSADVEFYQSE"
     gene            657..>1267
                     /locus_tag="VNV63_03930"
     CDS             657..>1267
                     /locus_tag="VNV63_03930"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HXC61803.1"
                     /translation="MKEIILCSLLVLCVYAARAQPAPPDADGISEAAPPKQIDRSRYN
                     LFNPTPSGEMRDFMPDRPSVTDGPYTVDPGHWLLELGLFEYTRDRDNNEGVRLDSFAS
                     GDTNIRLGLTSYAEVDLLFTAYSSILITAKDTGTRQRQSGFSDLTLRSKINIFGDDGG
                     PCAVGLIPFVTFPSGADGLGNRGFAGGVGFPVLFTLPAAFQLG"
BASE COUNT          299 a          338 c          325 g          305 t
ORIGIN      
        1 cactctgatt gatagaattc cacgtcggcc gacatttgac cgattcgaat cgacgctttg
       61 ccaccgaaaa atttctgctc aaaccagagc tcgaaaagtc tgacgctatc gtaagcgtcg
      121 atgctactaa ggatattaaa atcgccagta tatcgtctgg agagtccgtt cccgtgcgga
      181 tagagggccg aagcatagat agaggcaccg tcccaatgga cgatcttctg gagatcaagc
      241 cgaagtgtta acttgagcag accctcgtag gaggcgccct gctggatacc gccggagaga
      301 ttgccgaata tttcccccgt atattctcct gtaaaggtca ctccattctc ctcggtcatc
      361 gagtgaacct gggtaagaaa gtccgcctcc ttggtgcttc ctgcagaacc gtcaaaggta
      421 gaaacgccga aaatcagcac ggacagaatc ggcgcgatta ttctcgatgc cttcccttca
      481 gcggtcgaat ttcgcatctt cgaaatctgt ggtttcaaat tcgggactta ggttcaagag
      541 tcgaagagca acgtaaagag atttttaacg acgattttgt cacacccggc tttatgggtc
      601 taaagaaaca aatgaaactt tttaagcggt tcccacactt gctggaaaga accttcatga
      661 aggaaattat tctttgtagt ctccttgttc tttgcgtgta cgcggcccgc gcgcaaccag
      721 cgccacctga tgcggatggc atctcggagg ctgctccgcc caaacaaata gaccggagcc
      781 gatacaatct ctttaacccg actccctccg gcgaaatgag agacttcatg ccggatcggc
      841 cgagtgtgac cgacggcccg tacacagttg atccaggcca ttggctcctg gaactcggac
      901 tattcgagta cacacgcgat cgcgacaaca atgaaggtgt ccgccttgat agttttgcct
      961 ccggggacac gaatatccgc cttggtctga cgagttatgc agaagtcgat ctcttgttta
     1021 cggcctacag ctctattctg atcacggcca aagacaccgg cacccgtcag aggcaaagcg
     1081 gcttcagcga tttgacgttg agatccaaga tcaacatttt tggagatgat ggcggccctt
     1141 gcgcggttgg gctgattccc tttgtcacgt ttccgagcgg ggcagatggc ctgggcaacc
     1201 ggggttttgc cgggggcgtc ggattccccg tgctgttcac tcttccggcc gccttccagt
     1261 tgggaat
//
LOCUS       DATMKH010000323         1997 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3661257, whole genome shotgun sequence.
ACCESSION   DATMKH010000323 DATMKH010000000
VERSION     DATMKH010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3661257"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VNV63_03935"
     CDS             complement(<1..237)
                     /locus_tag="VNV63_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HXC61804.1"
                     /translation="MIAAVFDLDNTIILGKSVERRFFYRLLREQRIGFREIARMIGFL
                     IRNLPRFSPMLLHTHKLYLAGKSVSEMERLAAQYV"
     gene            479..1114
                     /locus_tag="VNV63_03940"
     CDS             479..1114
                     /locus_tag="VNV63_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXC61805.1"
                     /translation="MKINEIFKSIQGESTFAGQPCVFIRTTGCNLRCHWCDTAYAFYE
                     GTEMDLDSILHRVRDYQCDLIELTGGEPLLQKETPALIKILLDEGYRVLIETGGSLDV
                     GVLDPRTTVIMDLKCPGSAMTHEMRWENLKQLKSTDEIKFVIADRTDYLWAKDIVERD
                     ALADRHPVLFSPVFGVMDPRQLAAWILEDGLKIKLQLQLHKYIWEPETRGV"
     gene            1129..>1997
                     /locus_tag="VNV63_03945"
     CDS             1129..>1997
                     /locus_tag="VNV63_03945"
                     /inference="COORDINATES: protein motif:HMM:NF014808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HXC61806.1"
                     /translation="MEVKVKKGRIESEAAEVLVLTHFEGEKNLSPEVQGVDKALGGLI
                     KGLLASGEFQGKYLSTSLLHSQDKLPARRILLIGLGKHKDFQPDRVRQVMGRAACRVR
                     ELGIKSFTASIPARHTLKGSLTASAQVLVEGAILGLYQFTEYRTEARAEFKEVKEMVI
                     VEPEARQLAEVQVGARRGQIIAEATNFVRDMGNQPSNVLTPTRLARIAQDLAKEYGMS
                     CQILERSQIEKLGMGALAGVARGSQEPPKFIILEHHGSKRSKDKPVALIGKSITFDSG
                     GISIKPAEKMEQM"
BASE COUNT          483 a          508 c          555 g          451 t
ORIGIN      
        1 aacgtattga gcggccaatc gttccatttc gctcaccgac ttgcccgcaa ggtagagctt
       61 gtgggtatgc agaagcatgg gtgagaatcg tgggaggtta cggatcagaa agccgatcat
      121 acgcgcgatc tctcggaatc cgatccgctg ttcgcgcagg agacggtaaa aaaatcgccg
      181 ttctaccgat tttccgagga tgattgtatt gtccaggtca aagaccgcgg cgatcattgt
      241 gagcgctaac cttttttcta gtcttccaat aaaacttttt gattttaatc cctcaccctt
      301 tggcctgtca attaaattgg aggaccgctg tatgttgcca accaggaaag gtgacacttt
      361 tctttgactc actttaggac accacccagc ccttgaccta atgaactcga cttggtatga
      421 tggaaatgtt tctttattga atcataggac aacttttcct tgaaacaggc ttcccgacat
      481 gaagatcaac gaaatcttta agagcattca gggagaatcc acctttgccg gacagccttg
      541 tgtttttatt cggacgacgg gttgcaatct tcgctgccat tggtgtgata ccgcttatgc
      601 attttatgag gggaccgaga tggatttaga ctcgatcctt catcgggtcc gggattacca
      661 atgcgacctg atcgagctca ccggcggtga accacttctc cagaaagaga cgccggcgtt
      721 gattaaaata ctgctcgatg aaggttatcg ggtcctgatc gagaccggcg gcagcctcga
      781 cgtcggcgtg ctggatccgc gcacgaccgt gatcatggat cttaaatgtc cggggagcgc
      841 catgacccac gagatgcgat gggagaatct gaaacagctc aagtccaccg atgagatcaa
      901 gttcgtcatc gcggaccgca cggattatct ctgggcgaaa gacatcgttg aacgggacgc
      961 tctggcggat cgccatcctg ttttattttc accggtcttt ggtgtgatgg atccccgcca
     1021 gcttgcagcc tggatactgg aagacggatt gaaaatcaag ttgcagctcc aactacataa
     1081 atacatctgg gaacccgaaa cacgaggagt gtaaaccaaa ggagattcat ggaggtcaaa
     1141 gtcaaaaaag gcagaatcga gtctgaggcc gccgaggtct tggtcctcac acatttcgaa
     1201 ggcgaaaaga acctctcgcc cgaggtgcag ggcgtggata aagcgctcgg tggactgatc
     1261 aagggtctgc tcgccagcgg tgaattccaa gggaagtacc tttcaacctc tctcctgcac
     1321 tcgcaggaca agctgccggc acgacgtatc cttttaatcg gactgggcaa gcacaaggat
     1381 tttcagccgg acagggtccg ccaagtcatg ggtcgtgccg cctgccgtgt tcgcgagctg
     1441 gggattaaat ccttcacggc ctcgattccg gcgcgtcaca cgctcaaggg gtctttgacc
     1501 gcgtccgccc aggtcctcgt ggaaggagcg attttggggc tttatcaatt cacggagtat
     1561 cggaccgagg cgcgcgcgga gtttaaagag gtcaaagaaa tggtcatcgt ggaacccgaa
     1621 gcgcgtcagc tcgcggaggt tcaggtgggc gcccgccgcg gccagatcat tgcggaagcg
     1681 accaactttg tgcgagacat gggaaaccag ccctcaaatg ttttgacccc gacccgtctc
     1741 gcccgcatcg ctcaagacct ggccaaggag tacgggatgt cctgtcagat cctggaacgt
     1801 tcgcagatcg agaagctggg tatgggcgcc ttggctggag tggcccgcgg gagtcaagag
     1861 ccgcccaagt ttattatctt agaacaccac ggctccaaac gcagtaaaga taaaccggtg
     1921 gcgttgatcg gtaagagcat cacgttcgac agcggtggga tctcgatcaa accggctgaa
     1981 aagatggagc agatgaa
//
LOCUS       DATMKH010000324         1653 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4631177, whole genome shotgun sequence.
ACCESSION   DATMKH010000324 DATMKH010000000
VERSION     DATMKH010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4631177"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="VNV63_03950"
     CDS             complement(<1..545)
                     /locus_tag="VNV63_03950"
                     /inference="COORDINATES: protein motif:HMM:NF013112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HXC61807.1"
                     /translation="MKKTGDRIKVSLVSLGCSKNQVDSEVMLGLLKQDGFEFTPDTEK
                     AEVIIVNTCGFIDAAKEESIETLIELGKLKKEGRCKVLVAAGCLAQRYGDELLSELKE
                     LDAVVGTGDFPEIAKLCQKLSKKTVKRSEPSMMVSQPAFLYDVATPRLRLSPPHWAYV
                     KISEGCNYRCSFCSIPSFRGD"
     gene            complement(542..1411)
                     /locus_tag="VNV63_03955"
     CDS             complement(542..1411)
                     /locus_tag="VNV63_03955"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HXC61808.1"
                     /translation="MKSIRTHTPLRLGVHVSIAGKLSQAVERAQALGCNTMQIFSRSP
                     RGWDAKPFDPEELERFRVLRKKAEITPLAVHGSYLINLASVDPKLQSRSVKAFIEELK
                     RCDQLGAEYLIAHVGSCSDSTEEDGIRRVAKALKTVLKRARFKTMILLENTARERGDI
                     GYRIEQIGQLLDQVERHPQIGVCLDTCHLFAAGYDISKTDGVNRMADEVEKHIGLEKL
                     RFIHVNDSKKGLDCRVDRHQHIGQGGIGLEGFRAFVNHPVFRDIPMVLETPKDSPQDD
                     PINLKIMRELSRG"
     gene            1475..>1653
                     /locus_tag="VNV63_03960"
     CDS             1475..>1653
                     /locus_tag="VNV63_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXC61809.1"
                     /translation="MVNQSNTQNIRNVIIIGSGPAGLTAALYAARGNLKPLLIEGIHA
                     GGQLMITTDVENYPG"
BASE COUNT          370 a          483 c          403 g          397 t
ORIGIN      
        1 atgtcgccgc ggaacgatgg gatactgcag aaagaacaac gatagttgca tccctccgag
       61 atcttgacat acgcccagtg gggcgggctt aaacgcagac gcggagtcgc gacatcgtag
      121 agaaaggccg gttggcttac catcatcgat ggttccgatc gctttacagt cttcttggat
      181 aacttttggc acagcttcgc gatctccgga aaatcaccgg tcccgaccac ggcatcaagt
      241 tctttcagct ctgaaagaag ctcatccccg tatcgctggg ccaaacagcc tgcggcgacg
      301 agtaccttgc agcggccttc ttttttgagc ttgcctaact cgatcagtgt ctcaatggac
      361 tcctccttgg ctgcatcaat aaagccgcag gtgtttacta ttatcacttc ggccttctct
      421 gtgtctggcg tgaactcaaa cccgtcctgc ttcagcagtc cgagcatcac ctcggagtcc
      481 acctggtttt tggaacaacc caggctgaca aggctgacct ttattctgtc gccggtcttc
      541 ttcatccgcg gctcagctct ctcataatct taagatttat cggatcatcc tggggcgagt
      601 ctttcggtgt ctctaagacc atggggatat ctcgaaacac cgggtgattt acgaaggccc
      661 ggaaaccttc aagcccgatg ccgccctggc cgatgtgctg atgccggtcc acacggcagt
      721 caaggccttt cttcgagtca ttgacatgga tgaagcggag cttctccaaa ccgatgtgct
      781 tttccacctc atcggccatg cggttcacgc cgtctgtctt cgagatgtca tagcccgcag
      841 caaaaagatg gcaggtatct agacaaacac cgatctgagg gtggcgctcg acctgatcaa
      901 gcagctgtcc gatctgctcg atcctgtatc cgatatctcc ccgctcgcgc gcggtgtttt
      961 ccagcaatat cattgtcttg aacctggccc gtttcaaaac cgtcttgagc gccttcgcca
     1021 cacgacgtat accatcttct tccgttgaat ccgagcagct ccccacatgg gctatgagat
     1081 attcggcgcc aagctgatcg cagcgcttaa gctcttcgat aaacgccttc accgaccggc
     1141 tttggagctt aggatctacg gaagccaggt tgatgagata agacccgtgg accgcaagcg
     1201 gtgtgatctc cgcttttttt ctcagtacac gaaatctctc aagctcctca ggatcaaacg
     1261 gcttcgcatc ccagccgcgc gggctccggg agaaaatctg catcgtgttg catcccaggg
     1321 cttgggcccg ttccaccgct tgggagagtt tccccgcaat cgagacatgg acaccgagac
     1381 gcagcggcgt atgagttcgt attgatttca caggacgttc atgataacat aagttaaacc
     1441 acttcggcaa agagttatag aaaaggaatc attaatggtc aatcaatcca acacgcaaaa
     1501 catacgaaac gtcatcatta tcggttccgg tcctgccggt ctgaccgctg cgctctatgc
     1561 cgcacgcggc aatctcaaac cccttttgat cgagggcatc cacgccggcg gccagctcat
     1621 gatcacaacc gacgtcgaga actatccggg ttt
//
LOCUS       DATMKH010000325         1875 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1118351, whole genome shotgun sequence.
ACCESSION   DATMKH010000325 DATMKH010000000
VERSION     DATMKH010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1118351"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="VNV63_03965"
     CDS             complement(<1..442)
                     /locus_tag="VNV63_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012532059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination/repair protein RecA"
                     /protein_id="HXC61810.1"
                     /translation="MAEKDSKQKALELAVAQIEKQFGKGAIMKLGSGEALADIPAIST
                     GSLGLDIATGIGGFPRGRVVEVFGPESSGKTTLTLHAIAEVQKTSGVAAFIDAEHALD
                     VGYARKLGIKTDDLLISQPDTGEQALEIAETLVRSGALDLIVIDS"
     gene            complement(477..1064)
                     /gene="thpR"
                     /locus_tag="VNV63_03970"
     CDS             complement(477..1064)
                     /gene="thpR"
                     /locus_tag="VNV63_03970"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HXC61811.1"
                     /translation="MERQRLFISLDLPLGIKEAAGKVQERLQEAQTSKNIYWNIIWTR
                     PGGMHLTLKFLGEVEAGRIQEIQAALVESIHAFRPISIAVEGLGGFPTMHAPRVIWLG
                     IKEPSGELLSFQKRVDQAVAPLGFPPEMREFHPHLTLGRVKSPKGRDVLVQVLKALDP
                     GRLGEWRLEELNLMQSMIQPDGTVYTKLSMMKAEI"
     gene            complement(1072..>1875)
                     /locus_tag="VNV63_03975"
     CDS             complement(1072..>1875)
                     /locus_tag="VNV63_03975"
                     /inference="COORDINATES: protein motif:HMM:NF014517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HXC61812.1"
                     /translation="MEEMFKESATKFLEKVVDSSPGIYLKTVRSYGLSESKVNELIKD
                     LFLVNKAVQLGLVATSSGVDVRIQISRKRAEEAEALGQELIGLLKERLGDHIYGMDQT
                     AMETVVGGLLKAQAVTLAVAESCTGGLIGHRLTNVPGSSAYLDRVLVCYSNRSKMELL
                     GVPARTLREHGAVSAPAVTAMTEGILKTSCAHLALAVTGIAGPTGGTKEKPVGLVYIG
                     LASAGLPAGQTGNKHCRFYHFSGGREMIKLHASQRALDILRRWLMGLKF"
BASE COUNT          399 a          602 c          436 g          438 t
ORIGIN      
        1 ccgagtcaat tacaataaga tccagcgcgc cgctccggac cagcgtttca gcaatctcca
       61 gcgcctgctc ccctgtgtcc ggctgggaga tcagaagatc atctgttttg atccccagtt
      121 tccgggcata gccgacatcc agggcatgct cggcatcaat gaaagccgcc acgcccgatg
      181 tcttctgcac ctcggcaatc gcatgaagcg tcagagtagt tttgccggag gactccggtc
      241 cgaagacctc aactacccgt ccccgcggga aaccgccgat cccggtggcg atgtccaggc
      301 ccagagaccc ggtcgagatg gccgggatgt ccgccagcgc ctctccagaa ccgagcttca
      361 tgatggcacc tttgccaaac tgcttctcga tctgggccac cgccagttcg agcgctttct
      421 gtttactatc tttctcagcc atgattatct cctttatata attcgattat tgatccttaa
      481 atctctgcct tcatcatcga taacttggta taaaccgtcc cgtcgggctg gatcatgctc
      541 tgcatcaagt ttaactcctc caacctccat tcccctaagc ggcccggatc gagagctttc
      601 agcacttgca ccaagacatc acgacccttg ggcgacttga cacgtcccag cgtgaggtgc
      661 ggatgaaatt cgcgcatctc cggtggaaac cctaacggtg ccaccgcctg atccacccgc
      721 ttttgaaaac tgagcagctc gccgctcggc tctttgatcc ctaaccagat taccctgggc
      781 gcatgcattg ttggaaatcc gcccagcccc tcaactgcaa tggagatcgg ccgaaaagcg
      841 tggattgatt ccaccaatgc cgcctgaatt tcctgtatcc gtcccgcctc cacttcgccc
      901 aaaaatttta gagtgaggtg catccccccc ggtctggtcc agatgatgtt ccagtaaata
      961 tttttgcttg tctgtgcctc ttgaagccgt tcctgaacct tccccgcggc ctctttgatc
     1021 cccagtggaa gatctaggga gataaaaaga cgctgccgtt ccacacttct cctaaaactt
     1081 tagacccatt agccatcggc gtaatatgtc cagagctctc tgagaggcat ggagcttgat
     1141 catctcacgt cctcccgaaa aatggtagaa gcggcaatgc ttgttacccg tttgtccggc
     1201 aggcaggccg gcactggcca gaccgatata gaccaatccc accggcttct ctttcgttcc
     1261 accggtcggc cccgcgatcc cggtaaccgc taaggccaga tgggcacagc tcgtcttcaa
     1321 gatcccttcg gtcattgcgg tcaccgccgg agcgcttacg gcgccatgtt cccgaagtgt
     1381 ccgggccggc acacctaaaa gttccatctt ggagcggttg ctgtagcaga ccaagacccg
     1441 atccaaatag gctgagcttc cgggaacatt tgtcaggcga tggccgatca gtccacccgt
     1501 gcaggattcg gcaaccgcca gcgtgacggc ctgagccttc agcaacccgc cgaccaccgt
     1561 ctccatcgcg gtctgatcca ttccgtagat atgatctccc aaacgctctt tcaaaagacc
     1621 gatcagttct tggcccagcg cctccgcctc ctctgccctc tttctgctga tctgtatccg
     1681 gacatctact cctgaggagg tcgccacaag acccagctga accgccttgt tcacaaggaa
     1741 caggtcctta atcaactcgt tcaccttgga ctcggaaaga ccgtaacttc ttacggtctt
     1801 gagatagatg ccgggagagg agtccaccac tttttctaaa aactttgtcg cggactcctt
     1861 aaacatctct tccat
//
LOCUS       DATMKH010000326         2412 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1752250, whole genome shotgun sequence.
ACCESSION   DATMKH010000326 DATMKH010000000
VERSION     DATMKH010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1752250"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="VNV63_03980"
     CDS             <1..260
                     /locus_tag="VNV63_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HXC61813.1"
                     /translation="YATLALATDYDCWYHGEESVTVEAVIQMLHQNVEAAKRLIRTAV
                     PKIPSGRSCPCAVALKNAIITSADKIPQKTRERLKPLLGDI"
     gene            262..993
                     /locus_tag="VNV63_03985"
     CDS             262..993
                     /locus_tag="VNV63_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61814.1"
                     /translation="MIRWSNGWIVLAAFCAACATAQDRFVIHNEAQKIMAAPSFNLQV
                     LTTNGVAMLPVISKTAPEGVRNDTAFEVLQAFRHNLPNVSVLPVPDTMEILRQSHLDK
                     ELKTWLSAYQAGGPVDPALLRRIGLATARRYLLLVEVDQYKKESKPVDVSYSSLPMTG
                     PVKSPALSKPVAYDIFKDIKLAGRLWDSECRSIAWEGQGRARVIDESPNERIRMADLF
                     QTAVRNFISAFLGSGGMAKGDVQGC"
     gene            1005..1913
                     /locus_tag="VNV63_03990"
     CDS             1005..1913
                     /locus_tag="VNV63_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HXC61815.1"
                     /translation="MTLLVVGSVALDSVKTPFGEVEEVLGGSATYFSVAASYFTRVNL
                     VAVIGDDFPIDYLSVLKKRNINLEGLERTVGATFRWKGEYGYQLNEAKTLETQLNVFE
                     KFRPKLPESYRESDLVFLANIDPELQEEVLHQVRRPRWVVCDTMNFWIERKREALLKT
                     LKQVNILIINDGEARQLAEEPNLVKAARRIMSWGPQTLIVKRGEYGAVTFNGRKIFVS
                     PAYPLETVFDPTGAGDSFAGGFLGYLAGTGNFKDQGIRQAVIYGSVMASFNVESFSLD
                     RLRQLTPRVIQARYREFKRLTHFEEI"
     gene            1915..>2412
                     /locus_tag="VNV63_03995"
     CDS             1915..>2412
                     /locus_tag="VNV63_03995"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXC61816.1"
                     /translation="MKIIKSLSYISAILLLLFLVGCATSQSRLKRENEASAHYKLGIA
                     QLNENRTQEAFVEFQKSLEINPRNSDTHYALGHIYYYQGKYPEAIQEFKTVLKLDPEN
                     SEAHNYLGKLYEIDKSWDDAIHEYQSALKNPKYATPQFAHYNLGLVYDNKGQFADAVK
                     EFQEAL"
BASE COUNT          577 a          572 c          704 g          559 t
ORIGIN      
        1 gctacgcgac cttggcgtta gccaccgact acgactgttg gtatcatgga gaggagtcgg
       61 tgacggtgga ggcggtgatc cagatgctgc accaaaacgt ggaggcggcg aaacggctca
      121 tccggacggc agttccgaag atcccgtccg ggcgaagctg tccttgtgcc gtggcgctta
      181 aaaatgctat tatcaccagt gctgacaaga ttccccaaaa aacacgggaa cgattaaaac
      241 ccttgctggg agatatctga aatgattaga tggtcgaacg gttggattgt tttggccgct
      301 ttctgtgcgg cctgcgccac ggcgcaggat cgatttgtca tccataacga ggctcagaag
      361 atcatggcag ccccgtcgtt taatttgcag gtcctgacga cgaacggtgt ggcgatgttg
      421 ccggtgattt ctaagactgc cccggagggg gtccgaaacg ataccgcgtt tgaagtgttg
      481 caggctttca ggcataatct tccgaatgtg tcggtcctcc cggttcccga tactatggag
      541 attctgagac aaagccacct agacaaagaa ctcaagacct ggctttcggc ataccaggcc
      601 ggcgggccgg ttgatccggc tttgcttcgc cggatcggac tggccaccgc aaggcgttat
      661 cttctgctgg tcgaggtaga tcaatacaag aaagagtcga agcccgtgga cgtgagctac
      721 tccagtttgc ccatgacggg tcccgtgaag agcccggcct tgtctaaacc ggttgcatac
      781 gatatattta aagacataaa gctggcaggg cgtctttggg acagtgaatg ccgcagcatc
      841 gcttgggaag gacagggtag ggcccgggtg attgatgaaa gcccgaatga gcggattcgg
      901 atggcagacc tgtttcagac ggcggttcgg aattttatct ccgccttcct aggctccggt
      961 ggtatggcga agggggatgt ccagggatgt tgaagaaaaa gccattgacg ttgttggtcg
     1021 ttggctcggt cgcgctggat tccgtcaaga cacctttcgg agaggtggaa gaagttttgg
     1081 gggggtccgc cacttatttt tctgtggcgg caagctactt tacgcgtgtg aatcttgttg
     1141 cggtgatcgg cgacgatttt ccgatcgact atctttcggt gttgaagaaa cggaatatca
     1201 acctcgaagg cctcgagcgg actgtcgggg caacctttcg atggaaaggc gaatacggct
     1261 accagctcaa cgaggccaag accttggaaa cccagctcaa tgtctttgaa aagtttcggc
     1321 cgaaacttcc ggagagctat cgagagtccg atctggtctt cttagcgaac attgatccgg
     1381 agcttcaaga agaggtcctc catcaagtcc ggcgtccgcg gtgggttgtc tgcgacacca
     1441 tgaacttctg gatcgagagg aaacgggagg ccttgctcaa gacgctgaag caggtgaaca
     1501 ttctaatcat caatgatggt gaggcccgtc agctggccga ggagcctaat cttgtcaagg
     1561 cggcgcgccg gatcatgagc tgggggccac agacgctgat cgtgaagcgc ggggagtacg
     1621 gggcggtgac gttcaacggc agaaaaatat ttgtctcgcc ggcctatccc ctggagacgg
     1681 tctttgatcc gaccggtgcc ggggatagct ttgcaggagg ttttctggga tatctggccg
     1741 ggacggggaa ttttaaagac cagggcattc gccaggcggt gatctatggg agcgtgatgg
     1801 cctcgtttaa cgtcgagtct ttcagtctgg acaggctccg tcaattgacc ccgcgggtga
     1861 tccaggcccg gtatcgggaa ttcaaacggt taacacattt tgaggaaatt tagagtgaaa
     1921 atcataaaat ctctgtctta tatatcggca attcttctcc tgctgttttt ggtgggatgc
     1981 gcgacttctc agagcagatt gaaaagagaa aacgaggcat ccgctcatta caaacttggg
     2041 atcgcccagc tcaatgagaa ccggacacaa gaggcctttg tagaattcca gaaatcgctg
     2101 gagatcaatc ccaggaattc cgacactcat tacgctttgg ggcatattta ttattatcaa
     2161 ggcaagtacc cggaagcgat ccaagagttc aaaacagtct tgaagctcga tcccgaaaat
     2221 tcggaagcgc acaattactt aggcaagctg tatgagatcg acaaaagttg ggatgatgcc
     2281 atccatgaat atcaatcggc cttgaagaat cccaagtacg ccacgcctca gttcgcccac
     2341 tataatctcg gtcttgtata tgacaataag ggacagtttg ccgacgcagt caaggagttt
     2401 caagaggcgc tt
//
LOCUS       DATMKH010000327         1939 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4632118, whole genome shotgun sequence.
ACCESSION   DATMKH010000327 DATMKH010000000
VERSION     DATMKH010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4632118"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1282
                     /locus_tag="VNV63_04000"
     CDS             <1..1282
                     /locus_tag="VNV63_04000"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HXC61817.1"
                     /translation="GIAAEIHPKRIVMKNGITLLIVEQHSLPIVNIEVLVKAGSIYDP
                     EAKAGLANLAANLLDEGTRKRGSAEIAEAIDFVGGSLSTSGGEDYSTVSVRILKKDLN
                     LGLDLLADVLINPAFLQTELDRKRGETIGTILAEQDEPGIVAEKAFNDLIFSPHPYQR
                     PVKGLESTLPAITRADLIEFHERYYRPNNTLMAIVGDITEQEISDLVSKYFGAWEPKK
                     TKPPVVPRAHKLDHKTVRLIDKDLTQASIILGHLGIDRKNPDYYAVVVMNYILGGGGF
                     SSRMMAHIRDDRGLAYSVSSHFDAELYPGSFTASLQTKNATAMPAIQGLLEEIKKIRE
                     KPVTDQELEDAKAFLIGSFPLRLDTTGKLSHLLATAEFYDLGLDYLEKYPELIRSVTK
                     EDVLRVAKKYLDPERYALVVVAKQSEAKIGDLKP"
     gene            1328..>1939
                     /gene="larE"
                     /locus_tag="VNV63_04005"
     CDS             1328..>1939
                     /gene="larE"
                     /locus_tag="VNV63_04005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HXC61818.1"
                     /translation="MDQGYQRLQEILKEMESVMVAYSGGVDSTFLLKVAHDLLGDRAV
                     GVTEISPTLAPSELEEARQAALAIGAPHQFVFADKLDDPAFVRNDSRRCYHCKLGLYR
                     ELKIMAKEQGIPYIADGTNLDDISDIRPGLEAAKEMGIRSPLLEAGLHKSAIREMSRA
                     LKLPTWDKPASPCLSSRFPHGTTITLDGLDRVAQAEAILKGSGF"
BASE COUNT          477 a          523 c          538 g          401 t
ORIGIN      
        1 gggaatagcg gctgagattc accccaagcg aatcgtcatg aaaaacggga tcacacttct
       61 gattgtggaa caacattcgc ttccgatcgt taatatcgag gtgctcgtta aggcgggctc
      121 gatctatgat ccggaggcca aggcagggtt ggctaatctg gcggccaacc tcctggatga
      181 aggcacgcgc aagcgcggct ctgccgaaat cgcagaggca atcgattttg tcggcgggag
      241 cctctcgact tcgggcggcg aggactattc cactgtttcg gtccgcatac tgaaaaaaga
      301 cttgaacctg ggactcgatc tcctagccga tgtcttgata aatcccgctt ttcttcagac
      361 cgaactggac cgaaaacggg gcgagactat cgggacaatt ctggctgaac aggacgaacc
      421 ggggatcgtg gccgagaagg ccttcaatga cctgattttc tctccgcatc cgtatcaacg
      481 tcctgtgaag ggcctcgaga gcacactccc cgccatcaca cgagcagacc tgatcgagtt
      541 ccatgagcgc tattaccgtc ccaacaatac cctcatggcg attgtggggg atatcactga
      601 gcaagagatc tcggacctcg tgtccaaata ttttggggcc tgggaaccta aaaaaaccaa
      661 accgccggtt gtcccgcgcg cacacaagct ggaccataaa accgtgcgat tgattgataa
      721 agatctcaca caggccagca tcatactggg acatctcgga atcgatcgga aaaatccgga
      781 ttactacgcg gtggtcgtga tgaattatat actgggaggt ggcggtttct cttcccgaat
      841 gatggcccat atccgagacg atcggggttt ggcttatagc gtgagcagcc atttcgatgc
      901 agaactctat ccgggcagct tcacggccag ccttcagaca aaaaacgcca cggccatgcc
      961 cgccattcaa ggactgctgg aagagatcaa aaaaatccgg gagaagcccg tgaccgatca
     1021 ggagctggag gatgccaagg ccttcttgat cgggagcttt ccgctgcggc tcgacaccac
     1081 cggcaagtta tcccatcttc tggcaacggc agaattctat gatctggggc tggattacct
     1141 ggaaaaatat ccggagctaa tccggtcggt cacaaaagaa gacgtcctcc gcgtggcgaa
     1201 gaagtacctg gatccggaac gctatgccct cgttgtggtg gccaaacagt ccgaggccaa
     1261 aataggtgat ctaaaaccat aagcacgggc ccctccggga agcacgatgc cccctccggg
     1321 aaaaaatatg gatcaaggct atcaacgatt acaggaaatc ctcaaggaga tggaatcggt
     1381 tatggtggct tactccggag gcgtggacag cacttttctc cttaaggtcg ctcatgatct
     1441 cttgggggat cgggccgttg gtgtgaccga gatttctcca acgctcgcac cgtccgagct
     1501 cgaagaggcg cgtcaagcgg ccttggccat cggtgcaccg catcagtttg tgtttgccga
     1561 taaactagac gatcccgcct tcgtccgaaa cgattcgaga cggtgctatc attgcaagct
     1621 ggggctgtat cgcgagctca agatcatggc gaaagaacaa ggcattcctt atatcgcaga
     1681 cgggaccaac ctggatgaca tcagcgacat ccggcccggg cttgaagccg caaaagagat
     1741 ggggattcga agtcctctgc tggaggcggg tctccacaag tcagctatac gtgaaatgag
     1801 ccgtgctctg aaacttccga catgggacaa accggcctcg ccttgtctct cctcacgttt
     1861 ccctcacgga acgacgatca cgctcgatgg gctggatcga gtcgcccagg ccgaggcgat
     1921 actgaagggg agcgggttt
//
LOCUS       DATMKH010000328         1258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3361148, whole genome shotgun sequence.
ACCESSION   DATMKH010000328 DATMKH010000000
VERSION     DATMKH010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3361148"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1258)
                     /locus_tag="VNV63_04010"
     CDS             complement(<1..>1258)
                     /locus_tag="VNV63_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXC61819.1"
                     /translation="ERYQKLLAEPEAEVEELEEMEELEEELEEGEKEKEKEIDLTPGI
                     LSRTDDPVRLYLKEMGSVPLLSREGEIEIAKRIEEGKREIASVVFGMPMTVKEVLFLG
                     DQLKKEKASIREIVSSFDEEFEETAEQDDKELTQKTIMAIRDVRGLYRQLLKLGSRWR
                     RAGQNPIRRRKIKAELKEIRAKIVEKMEAINLHPTLVERMVQRVKSLARETQLYEREI
                     TNCKRRLGVAEDEAVHHLLARVRRGGKYLQAVRRKTGLSKETLHQIENLYRAARKKLR
                     GIEVEALVTADELKEAVRLLETAEQKMKQGKSELIEANLRLVVSIAKKYTNRGLQFLD
                     LIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRAIADQARTIRIPVHMIETIN
                     KLIRTSRHLVQELGREPTPEEIGEKMT"
BASE COUNT          238 a          384 c          291 g          345 t
ORIGIN      
        1 cgtcatcttc tccccaatct cttcgggggt cggttcccgc cccaattctt gaaccaggtg
       61 acgcgaggtc cggatgagtt tattaatcgt ctcgatcatg tgaaccggga tacggatcgt
      121 ccgggcctgg tccgcaatgg cccgcgtgat cgcctgtcgg atccaccagg tggcataggt
      181 gctgaatttg tagccacgtc ggtattcaaa tttatccacg gccttcatga ggccgatatt
      241 tccttcctga atcaggtcca aaaattgtag accccggttg gtgtatttct tcgcgatgct
      301 gacgaccaat ctcaggttgg cctcaatgag ttcactcttc ccttgcttca ttttctgctc
      361 ggccgtttca agcagacgta cagcctcctt gagctcatcg gcagtcacca gcgcctcgac
      421 ctcgatgcca cgtagtttct tacgcgcggc acgatagaga ttttcaatct gatgtagggt
      481 ctctttggag agtccggtct tccggcgaac cgcctggagg tactttccgc ctcgccggac
      541 acgagcgagg aggtgatgaa ccgcctcatc ctccgcaacg cccaatcgtc gcttgcaatt
      601 ggtgatctcc cgctcataca gctgagtctc tcgggccagc gacttgacac gttgaaccat
      661 tcgctctaca agggtgggat gaagattaat ggcctccatt ttctcaacga tcttagcacg
      721 gatctcttta agctccgcct taattttgcg gcgccgaatg ggattctgac cggctcgacg
      781 ccagcgactg cccagtttca ataattggcg atacaatccg cgaacatctc gaatcgccat
      841 gatcgtcttt tgggtaagct ctttatcgtc ttgttccgcg gtctcttcga actcttcatc
      901 aaaagaagat acaatttccc ggatacttgc cttctctttt ttcagctgat cacccagaaa
      961 gagtacctcc ttgacggtca tgggcatgcc gaagacgacc gaggcgattt ccctctttcc
     1021 ttcctcgatc cgcttcgcga tctcgatctc tccctcacga cttagcaatg gaacactgcc
     1081 catctctttg aggtagagcc gaacgggatc gtcggtccgg ctgaggatcc ccggagtgag
     1141 atcgatctcc ttttcctttt ctttctcccc ctcctccagc tcctcctcca gctcttccat
     1201 ctcctcgagt tcttccactt cggcctccgg ttcggccaga agcttctgat accgttca
//
LOCUS       DATMKH010000329         1007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4632546, whole genome shotgun sequence.
ACCESSION   DATMKH010000329 DATMKH010000000
VERSION     DATMKH010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4632546"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1007)
                     /locus_tag="VNV63_04015"
     CDS             complement(<1..>1007)
                     /locus_tag="VNV63_04015"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HXC61820.1"
                     /translation="KDLHGVNWDYYKGVYQKFLPFISDNRDFAEMESELLGELNASHT
                     GCYYTPEMEGTDATAALGAFFDPNFKGDGLLIQEVIEKGPLVTAAAEVRAGMIIIKID
                     GTQITQGMDISPLLNHKGGQLTLVTILDPTTNNRVEVAIKPISLGDQQELLYQRWVKQ
                     RRTMVDKLSNGTIGYVHVRGMTDESFRETYSQALGRESGKTALIVDTRFNGGGNLHDE
                     LATFLSGRRYLENFPRGQSLGWEPDGKWTRKSVVLAGESNYSDADVFPWVYQYLNIGK
                     LVGMPIPGTGTSVWWERQIDPTLVFGIPEVGLRDPEGQFLEKIQVEPSVLIANDPGTL
                     S"
BASE COUNT          215 a          295 c          236 g          261 t
ORIGIN      
        1 gggacagagt gccgggatca ttcgcaataa gcacactcgg ttcaacctgg atcttctcca
       61 gaaattgtcc ctccggatct cgcagaccaa cttccggaat tccaaaaacc agcgttggat
      121 cgatttgccg ttcccaccag accgatgtcc ctgtgccagg aattggcatt cccacaagct
      181 tgccgatatt caggtactgg tagacccacg gaaaaacatc ggcatccgag tagttgcttt
      241 cgcctgctaa aaccacgctc tttcttgtcc actttccgtc cggttcccag ccgaggcttt
      301 gaccgcgggg aaaattttcc aggtaccgtc tcccgcttaa aaacgttgca agctcgtcgt
      361 gcagattacc gcctccattg aatcttgtat ccacaatcag agcggtttta ccgctttccc
      421 ggcccagggc ctgcgaatat gtttctcgaa agctctcgtc ggtcatgccg cgaacatgga
      481 catagccgat cgttccattg ctgagtttgt cgaccatggt tcggcgctgt ttgacccatc
      541 gttggtacaa caattcctgt tgatccccga gactgatcgg cttaatggcg acttccactc
      601 ggttgttggt cgtagggtcg aggatcgtca ccaacgtcaa ttggcccccc ttgtgattta
      661 gaagagggct gatatccatc ccctgagtga tctgtgtgcc gtcgatcttg ataatgatca
      721 tacccgcccg aacttcagcg gccgcagtta ccagcggtcc cttttcaata acttcctgaa
      781 tcaggagacc gtctcccttg aaattcggat cgaaaaacgc cccaagagcc gcggttgcat
      841 ccgtcccttc catttccggt gtgtaataac agcccgtatg gctcgcgttg agttcaccca
      901 gcaattcgct ctccatctcg gcgaagtccc ggttgtcgct gatgaaagga agaaattttt
      961 ggtaaacccc cttgtagtag tcccagttca caccgtgcag gtctttg
//
LOCUS       DATMKH010000330         1396 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3663403, whole genome shotgun sequence.
ACCESSION   DATMKH010000330 DATMKH010000000
VERSION     DATMKH010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1396
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3663403"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VNV63_04020"
     CDS             complement(<1..207)
                     /locus_tag="VNV63_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HXC61821.1"
                     /translation="MSESDKSQWFVVHVLSGQEQKVFDNLARRIKTEEMGDLVYEVLI
                     PTERVSEIKRGKRTETTRKFFPGYL"
     gene            complement(225..437)
                     /gene="secE"
                     /locus_tag="VNV63_04025"
     CDS             complement(225..437)
                     /gene="secE"
                     /locus_tag="VNV63_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018970128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HXC61822.1"
                     /translation="MISRVRGFLTEVRTELEKASWPWDPKEKGIKKYRELIDSTAVVV
                     VAMLLLSGYIAFWDLVMVNLVKLLVP"
     gene            complement(514..586)
                     /locus_tag="VNV63_04030"
     tRNA            complement(514..586)
                     /locus_tag="VNV63_04030"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(551..553),aa:Trp,seq:cca)
     gene            complement(675..>1396)
                     /locus_tag="VNV63_04035"
     CDS             complement(675..>1396)
                     /locus_tag="VNV63_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012463134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HXC61823.1"
                     /translation="DLLSQYEFPGDTIPIVKGSALKALEGDPKSKEAIYQLMAAVDEY
                     IPLPERPIDQPFLMPIEDVFNIEGRGTVVTGRVERGILKKMEEVEIVGIRTTAKTTVT
                     DIEMFRKLLDEARAGDNVGVLLRGIKKEDVERGQVVAKPGTIHPHKKFKAEVYVLSKE
                     EGGRHTPFFTNYRPQFYFRTTDVTGTVKLPDGVEMVMPGDNVGIEVELLTPIAMERTI
                     RFAIREGGRTVGAGRVADILD"
BASE COUNT          320 a          430 c          301 g          345 t
ORIGIN      
        1 caggtatccg ggaaaaaatt tacgagtcgt ttcggtccgc tttcctcgtt tgatttcgga
       61 cactcgctcc gtcggaatca atacctcgta caccaggtcg cccatttcct cggtcttaat
      121 tcgacgcgcg agattatcaa agactttctg ctcctgccct gagagcacat gcacgacgaa
      181 ccactgactt ttatcagatt cactcatatc gagtcaccta cgactcatgg aaccaacaac
      241 ttcacgagat tcaccatcac cagatcccag aacgctatat acccgctcag aagcaacatc
      301 gcgacaacga ccaccgctgt tgaatcgatg agttcgcggt acttcttaat tcccttttcc
      361 ttgggatccc aaggccagct ggctttttcc agctctgtcc ttacctcggt caaaaaccct
      421 cttacacgac tgatcatact gttaaacgaa gtatttaact ccgcttattt catctttcca
      481 caggactggc acgatccggc aacctctcat gaacaggtca ggagggactc gaacccccaa
      541 ccggcggttt tggagaccgc tgctctacca attgagctac tgacctaaac tttatcactc
      601 cggaaaaaca agcgggtccg gcatacgccg gaccacctgt cttctgcaaa atattacctc
      661 ccgacctcag cacgctagtc aaggatgtcc gccacccggc ccgcgccaac cgttcggcca
      721 ccctcgcgaa tggcaaagcg gatagttctc tccatggcaa tcggagtcag caattccact
      781 tcgattccga cgttgtcacc cggcatcacc atttcaactc cgtccggcaa cttcaccgtt
      841 cccgtcacgt ctgtcgttcg aaaataaaac tgcggacgat agttggtgaa aaagggagtg
      901 tgccgaccgc cttcttcctt gctgaggacg tacacttctg ccttgaactt cttgtgagga
      961 tggatggtgc ccggcttggc aacaacctgt ccccgttcga catcttcctt cttaatgcca
     1021 cgaagcaaaa caccgacatt gtcgccggcg cgcgcttcgt cgaggagctt gcggaacatt
     1081 tcgatatccg tcaccgtcgt cttcgcggtt gtgcgtatcc ctacgatctc gacttcctcc
     1141 atttttttca gaataccgcg ctccacacga ccggtcacga ccgttccacg accttcaatg
     1201 ttaaatacat cttcgatcgg catgaggaag ggctgatcaa tcgggcgttc cggaagcggg
     1261 atatattcat cgacggctgc catcaactga tagatcgcct ctttggattt tggatcacct
     1321 tccagggctt tcaacgcgct ccctttaaca atcgggatcg tgtccccggg gaactcatat
     1381 tgcgagagga gatcgc
//
LOCUS       DATMKH010000331         1606 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3362484, whole genome shotgun sequence.
ACCESSION   DATMKH010000331 DATMKH010000000
VERSION     DATMKH010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3362484"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1160
                     /gene="carB"
                     /locus_tag="VNV63_04040"
     CDS             <1..1160
                     /gene="carB"
                     /locus_tag="VNV63_04040"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546879.1"
                     /note="four CarB-CarA dimers form the carbamoyl phosphate
                     synthetase holoenzyme that catalyzes the production of
                     carbamoyl phosphate; CarB is responsible for the
                     amidotransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HXC61824.1"
                     /translation="PRSGTARSFAEARLVASEIGYPVMVRPSYVLGGRAMEVVYDENS
                     LRNYMTDAVKASPKHPILIDKYLEDAIEVDVDAVSDGTRTVIGGILEHIEEAGVHSGD
                     SACSLPPFSLKSSILDEMRRQTICLAKEMKVIGLMNAQYAVKDGEVYVLEVNPRASRT
                     VPFVSKAIGVPLAKIAMKVMIGRDLKSLGFVTERSLKHVAVKEAVFPFTKFPGVDTIL
                     GPEMKSTGEVMGIDEEFGRAFAKSQDAAGNPLPLSGRVFISVKDKDKQPVVEIARRLT
                     ALGFTIEATQGTAEFLRKHQVPVDTVNKVAEGRPHIVDHMKNREIHLMINTVGDKQAQ
                     VDSYVIRRTALNQNIPYFTTIAEAKAVVRGIEAGLKEGMKIKSIQAYHLEI"
     gene            1188..>1606
                     /gene="greA"
                     /locus_tag="VNV63_04045"
     CDS             1188..>1606
                     /gene="greA"
                     /locus_tag="VNV63_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HXC61825.1"
                     /translation="MKIPMTRKGHEQIREELERLRKIERPKVILAISEARAHGDISEN
                     AEFLAAKERQSFIEGRIKELETQLVDAEVIDTSQLPSDKVVFGTTVTLLDVETKQKKQ
                     YTLVGQGEADLKNGQISVQSPVGRALIGHKAGDKVTVS"
BASE COUNT          413 a          368 c          463 g          362 t
ORIGIN      
        1 agccgcgaag cggaaccgct cgttcattcg cggaggcccg actggtcgcg tccgagatcg
       61 gttatcctgt catggtcagg ccttcttatg tcctgggcgg ccgtgcaatg gaggtcgttt
      121 acgatgagaa tagcctccgg aactatatga ccgatgcggt caaggcctcc cccaagcacc
      181 cgatccttat agacaagtac ctggaggatg ccattgaagt ggatgtggat gctgtctcgg
      241 atgggactcg tacggtgatc gggggtattc tggagcacat cgaagaggcc ggcgtgcatt
      301 ccggagactc ggcctgctca ctgccgccgt tttcactgaa atcttctata ctggatgaga
      361 tgcgccgtca aacaatttgc cttgccaagg agatgaaagt tatcggcctg atgaatgccc
      421 agtatgccgt caaggacggc gaggtgtatg tcctggaagt caatccccgc gcttcgagaa
      481 ccgttccgtt tgttagcaag gccattgggg ttccattggc caagattgcg atgaaggtca
      541 tgatcggacg agatcttaag tctctcggtt ttgtgaccga gcgttcctta aaacatgttg
      601 cggtcaagga ggctgtgttt ccctttacta aatttccagg cgtagacact atcttaggcc
      661 ccgagatgaa atctaccggt gaggtcatgg gaattgatga agagtttggc cgtgcctttg
      721 ccaagtccca ggacgcggca gggaaccctt tgcccttaag cggccgtgtc tttatcagtg
      781 ttaaagacaa ggataagcag cctgtggttg aaatcgcaag gcgtctcact gcgcttggat
      841 ttacgattga ggccacgcag gggaccgcag aatttctccg gaagcatcag gttcctgtgg
      901 ataccgtcaa caaggtcgca gagggaaggc cgcatatcgt ggatcacatg aaaaatcggg
      961 aaatccacct gatgatcaac acggtcgggg acaagcaggc tcaggttgat tcctatgtga
     1021 tccggaggac cgccttgaac cagaacatcc cttactttac caccattgca gaggccaagg
     1081 cggtggttcg aggaatcgag gccggattaa aagaggggat gaagatcaaa tctatacagg
     1141 cttaccactt agagatttga gaaatagtat cttaggagga agttgtagtg aagattccaa
     1201 tgacaagaaa aggccacgaa caaatccgtg aggaactgga acggctccgg aaaattgaaa
     1261 ggccaaaggt gatcctggca atcagcgagg cccgggcgca tggtgacatc agtgagaatg
     1321 ctgagttctt ggctgcaaaa gagcggcaaa gttttataga agggcggatc aaagaacttg
     1381 agacccaatt agtggatgcc gaggtgattg acacctcaca gttaccttcg gataaggttg
     1441 tttttggaac caccgtgact cttttagacg tcgagacaaa acaaaagaaa cagtacacat
     1501 tggttgggca gggtgaggcg gatcttaaaa acgggcagat ttcggttcaa tcccctgtgg
     1561 gcagagccct gatcggacat aaagcaggcg acaaggtcac agtctc
//
LOCUS       DATMKH010000332         1772 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4634631, whole genome shotgun sequence.
ACCESSION   DATMKH010000332 DATMKH010000000
VERSION     DATMKH010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4634631"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="VNV63_04050"
     CDS             complement(<1..453)
                     /locus_tag="VNV63_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61826.1"
                     /translation="MTVLYVSRQTTLQESRDLTANALRELYSYVADLGLLICGPQYRF
                     FYGMGGQPDSPLTMEIALPVQGQIPTTLLPFFKQIPPFNCLCSRYEGPWEGLITEYSE
                     MLDYVAFNDLEMTDLYVETLLHIDYAAPACQLTEIQIGLAPTVRAPKTV"
     gene            complement(526..1479)
                     /locus_tag="VNV63_04055"
     CDS             complement(526..1479)
                     /locus_tag="VNV63_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009282424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HXC61827.1"
                     /translation="MNRIDRLHAILTHLQSKKKVTAQEMADRFQISLRTVYRDVKALD
                     ESGVPVIGEAGSGYTIMEGYRLPPVMFTQEEASALLLGAKLAEQFTDGSVKRHFNAAL
                     FKIKAVLRSPDKEYVDSLTEHIEILIRYAPDLESPRQYLSMLQQAVVGKKIVHLQYRS
                     QQKDEVTLRKVEPIGLLYYGNAWHLIAWCHLRNDYRDFRMSRMLGVTVDDHCFEPSAH
                     PSIREYIDKIRQSSDLEEAIVCFDREVAKYLQEQKYLNGFVSEEVQDAGVRMKFLTSN
                     LGHFGRWLLSFTNRVQIESPPALRTAMKELAEKISRHYGSY"
     gene            complement(1490..>1772)
                     /locus_tag="VNV63_04060"
     CDS             complement(1490..>1772)
                     /locus_tag="VNV63_04060"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010856632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HXC61828.1"
                     /translation="TLFDLQETTAKIRAVTHHLKGVKIAIMGCIVNGPGEMADADFGY
                     VGSGPGKITLYKGKEIVKRGVDSSIALEELINLLKDSEAWVDPLPVAIS"
BASE COUNT          388 a          496 c          472 g          416 t
ORIGIN      
        1 caccgtcttt ggcgcccgga cagtcggagc aagcccgatc tggatctcgg tcaactgaca
       61 ggcgggtgca gcatagtcga tatgcagcag ggtctccaca tagagatcgg tcatttccag
      121 atcgttgaaa gcgacataat ccaacatctc cgaatattcc gttatcaagc cctcccaggg
      181 accttcatac cgggaacaca gacagttgaa aggcgggatt tgcttgaaga agggcaggag
      241 ggtcgtcggg atctgaccct gtaccggcaa tgcgatctcc attgtcagcg ggctgtccgg
      301 ctggccgccc atcccataga agaaacggta ttgcggcccg cagatcagga gccccagatc
      361 ggcaacgtag gagtacaatt cccgtaaagc attggcggtc aggtccctcg attcctggag
      421 agtagtctgg cggctgacgt aaagaacggt catgggagga tggacgcgga taagcataga
      481 tttctggttt ctccaaagat gcgcccggtt tgtgtcagcc ttatgtcagt aggagccgta
      541 gtgcctggaa attttttctg caagctcctt catggcggtc cggagagcgg gcggagactc
      601 gatctgtacc cggtttgtaa agctcagtaa ccagcgcccg aaatggccca gattggacgt
      661 caggaatttc atccggactc ccgcatcctg cacttcttcc gatacgaatc cattcagata
      721 cttctgttcc tgaaggtatt tggccacctc ccggtcgaag cagacgatgg cttcttccag
      781 gtcggaactc tgcctgatct tgtcgatata ctcccggatg gaaggatgag cggacggctc
      841 gaaacaatga tcgtcgaccg tgacccccag catccggctc attcgaaaat cccgatagtc
      901 attgcgcagg tgacaccagg cgatcagatg ccaggcgttc ccatagtaaa gcaggccgat
      961 cggctccact ttcctgaggg tgacttcgtc tttctgctgg gagcggtatt gcaggtgaac
     1021 gatctttttg cccacgaccg cctgctgcaa catcgacaag tattgccgcg gggactcgag
     1081 gtcgggtgca taccggatca ggatctcgat atgctccgtc agactgtcta catattcttt
     1141 gtccggagat cgcagtaccg cctttatctt gaacagggcc gcattgaagt gccgctttac
     1201 cgacccatcg gtgaattgct cggccagttt agcccccagc agcagcgcgc tggcctcttc
     1261 ctgagtgaac atgaccggcg gcagccggta accttccata atggtatagc cggagcctgc
     1321 ctcaccgatg accggtacac cggattcatc cagggctttg acgtcccggt agaccgtccg
     1381 caggctgatc tgaaagcggt cggccatttc ctgagccgtc actttctttt tgctctgcaa
     1441 atgcgtgagg atggcgtgca gcctgtcaat tcggttcacc ggaaagtttt taagaaatcg
     1501 caacaggaag tggatcgacc caagcttcgc tgtccttcag taaatttatc aactcttcaa
     1561 gggcaatgga gctgtcgacg cctcttttta caatttcttt acccttatac agcgtgatct
     1621 tgcccggacc gcttcctaca tacccgaaat ccgcatcagc catttcaccc ggcccattga
     1681 cgatacaacc catgattgcg atcttcaccc ctttgaggtg atgggtcaca gcccggatct
     1741 tcgcggtagt ctcttgcagg tcgaagagcg ta
//
LOCUS       DATMKH010000333         1286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1439555, whole genome shotgun sequence.
ACCESSION   DATMKH010000333 DATMKH010000000
VERSION     DATMKH010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1439555"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..875)
                     /locus_tag="VNV63_04065"
     CDS             complement(<1..875)
                     /locus_tag="VNV63_04065"
                     /inference="COORDINATES: protein motif:HMM:NF024779.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HXC61829.1"
                     /translation="MPRLSAGAEVSLITYTPGEELYQSFGHSSIRVRDELLGLDRLYN
                     FGVFDFETPNFYVKFAHGDLLYQLAVSQGEEEIRVVGAYGQGVSELLLNLALSQRQSL
                     FEALEINLRPENRFYRYDFILDNCSTRPRDVIERITGSPVVVPGAGKQTFRDMLDPYF
                     IRMPWVGLGVSLLMGANVDRPASPREACFLPADLERAVQASKNGEQSLAGEKKEIFAA
                     ETFPGPSPFLSPTSVFYSVGILWILFWLLRRKGHARWPTAIALMILGALGLFVLGLSF
                     WTRLWVLHRNYNLWWL"
     gene            complement(1056..>1286)
                     /locus_tag="VNV63_04070"
     CDS             complement(1056..>1286)
                     /locus_tag="VNV63_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009059869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HXC61830.1"
                     /translation="SEGDLLPKEKLTIFELADARRAAVRPSGTEPKIKFYLFGKRSDI
                     SADSLSRVKADLAGALESLWEWLQKDAKERVS"
BASE COUNT          313 a          366 c          344 g          263 t
ORIGIN      
        1 agccaccaaa gattgtaatt tctatgcagt acccagaggc gcgtccagaa ggagaggccc
       61 agaacaaaaa gccccagagc gccgaggatc atcaaggcaa tcgctgtcgg ccacctggca
      121 tgccctttcc tgcgcaacag ccagaaaaga atccagagaa ttccgacgct gtaaaaaacg
      181 ctcgttggtg ataggaaagg cgacgggcca gggaaggttt ccgccgcgaa gatttctttc
      241 ttttccccag ccaggctttg ctctccgttt ttgctggcct gtaccgcgcg ttcaagatct
      301 gccggcagaa agcatgcctc tcgcgggctt gccggtcgat cgacgtttgc tcccatcaac
      361 agtgaaaccc ccagcccgac ccagggcata cggatgaaat agggatccaa catgtccctg
      421 aaagtttgct tgccggcgcc gggaacaacc accggagaac cggtgatccg ttcgatgacg
      481 tcccgtggac gagttgagca gttatccagg atgaagtcgt accggtagaa ccggttctcc
      541 ggacgaagat tgatctcgag ggcttcgaac aatgattgtc tctgggagag cgcgagattc
      601 aacagaagct ctgagacgcc ctgaccgtac gctccaacca cccgaatctc ctcttcccct
      661 tgtgagacgg ccagttggta gagcaaatcg ccatgagcga atttcacata aaaattcggg
      721 gtctcgaaat caaagactcc gaagttatac aagcgatcca ggccgagcag ttcgtccctc
      781 actctgatgg agctatgacc gaaggactgg taaagctcct cgcccggggt gtaagtgatc
      841 agagaaacct ctgcccctgc gctgagccga ggcaaccggc cgccaagatc ctctagggta
      901 ggcgcggcgt cctgcgcgga tgccggctgc ctggaaagga cgagaacgaa aagtaaaatc
      961 gctcgaaaga agcctataac aagcgtttgc ctctctagct ttaggggcgg gagaaacaac
     1021 cgatctcctc gaaatgtcca aaccatgtat tcgcgctagc tgactctctc tttagcgtct
     1081 ttctgtagcc actcccagag gctctccaaa gcgccagcaa gatcggcttt gactcgactc
     1141 agcgaatctg ccgaaatatc ggatctcttg ccgaacagat agaatttgat tttcggttcg
     1201 gtgccggacg gtcgtacggc agcccgcctg gcatcggcga gctcaaagat cgtcagcttc
     1261 tcttttggca gaaggtcccc ttcgga
//
LOCUS       DATMKH010000334         1325 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2393644, whole genome shotgun sequence.
ACCESSION   DATMKH010000334 DATMKH010000000
VERSION     DATMKH010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2393644"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VNV63_04075"
     CDS             complement(<1..188)
                     /locus_tag="VNV63_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013581222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HXC61831.1"
                     /translation="MKKIASATDPLWYKDAIIYELHVRAFADSNNDGIGDLPGLISKL
                     DYLQDLGVSCIWLLPFFPS"
     gene            complement(336..>1325)
                     /locus_tag="VNV63_04080"
     CDS             complement(336..>1325)
                     /locus_tag="VNV63_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002810452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HXC61832.1"
                     /translation="YAENPPKKYQDIYPFDFESDDWDGMWEELKSVFTYWIGVGVTIF
                     RVDNPHTKAFPFWEWVITEIKRDHPEVLFLAEAFTRPKIMYRLAKLGFSQSYTYFPWR
                     NGKYEIASYLTELAQTPVREFFRPNQWPNTPDILTEFLQMGGRPAFVIRLLLAATLGA
                     NYGIYGPAFELQENKPVKHGSEEYLNSEKYEIRTWDLARPDSLRDLIARVNAIRNENP
                     ALQNDWSLKFHSTDNDQLICYSKESDDRSNLLVTVVNLDPHHTQAGFVVLPLDDLEIP
                     QDRPFEAEDLLTGERYLWQGPRNYVELNPSTRSGHILKIHRRMRVETDFEYFL"
BASE COUNT          309 a          352 c          353 g          311 t
ORIGIN      
        1 gaaggaaaga aaggcagcag ccagatacag gaaaccccga gatcctgcaa ataatcgagc
       61 ttcgatatca acccaggtaa atctccgatg ccgtcgttgt tggagtcagc gaaggctcgc
      121 acgtgaagct cgtagatgat cgcgtccttg taccagagcg gatcagtagc gcttgcaatc
      181 ttcttcacgc agcgggtctc caagctgatt tgatgcacct gaacgactgg cgtactggag
      241 ggggcatatg ttgcgcccga agcatgtcat ctttagttga tgcctcttcc ggcgacgccg
      301 gtgtctgaaa agccattgcg gtcgagtcga caaacctaaa gaaagtattc gaaatccgtt
      361 tccacccgca tacggcgatg gattttgaga atgtggcctg acctggtcga gggattcaac
      421 tccacgtaat tccgcgggcc ctgccacaag tatcgctcac ctgtcagcaa gtcctcggcc
      481 tcgaaggggc gatcctgagg tatttcgagg tcatccagag gcagaacaac aaaacctgcc
      541 tgtgtgtggt ggggatcgag gttaacgact gttacaagta ggttcgaccg atcatccgat
      601 tccttgctgt aacaaatcag ttggtcgttg tcggtggaat gaaattttaa gctccaatca
      661 ttctgaagag ctggattctc attgcgaata gcgttcacgc gtgcaatcaa gtcgcgaagg
      721 ctgtcgggcc gggccaggtc ccatgttctg atctcgtatt tctctgaatt gaggtattct
      781 tcgctaccat gcttgaccgg cttgttctcc tgcaattcga aggccggacc atagattcca
      841 tagttggctc cgagcgtcgc tgcgagcagc aatcggataa cgaaggcggg acggcctccc
      901 atctgaagaa actcggtcag gatatccggc gtattcggcc actggttcgg cctgaagaac
      961 tcccgtaccg gcgtctgcgc cagttccgta aggtaggaag cgatctcgta cttcccatta
     1021 cgccacggga aatacgtgta cgactggctg aatcccagct ttgccagccg atacatgatc
     1081 ttggggcggg taaaggcttc tgcgagaaag agcacctccg gatggtcccg tttgatctct
     1141 gtaatcaccc actcccagaa gggaaacgcc ttggtgtgcg gattatcaac gcggaagatc
     1201 gtgacgccca caccgatcca gtaggtgaag acgcttttca actcctccca catcccatcc
     1261 cagtcgtcgc tttcaaaatc gaatggatag atgtcctgat acttcttcgg cggattctcc
     1321 gcata
//
LOCUS       DATMKH010000335         3268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_159520,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000335 DATMKH010000000
VERSION     DATMKH010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159520"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(187..918)
                     /locus_tag="VNV63_04085"
     CDS             complement(187..918)
                     /locus_tag="VNV63_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HXC61833.1"
                     /translation="MKRSGIVIFGFGLALVAVFAVILAGLGSHWGWWDFRTGFTIMKW
                     GGFGSLAAAVVSLIGSILVRPGRSRQGFMLGLAGLVIGFVVVCVLLQWMWTAKHVPPI
                     HDITTDTENPPAFVSILPLRTGAANPAEYGGPEIAAQQHKGYPNLGPLMLDVPPDQAF
                     RRALEAARAMGWEIVASNPADGRIEATDTTFWFGFKDDVVVRITPAEHGSRIDVRSVS
                     RVGRSDVGTNAKRIQKYLEKIKQLS"
     gene            complement(929..2473)
                     /locus_tag="VNV63_04090"
     CDS             complement(929..2473)
                     /locus_tag="VNV63_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HXC61834.1"
                     /translation="MAIEIVINETREETRVALLENRVVTELYIDRRKEQGIVGNVYKG
                     KASKVLPGMQAAFVDIGQEKAAFLHASDITGAPDEYPGPVEEPTLDGSEEKEETQSLT
                     ETETTPRSRRSSSLPIEELLQEGQEVLVQVSKEPMGTKGSRVTTQISLPGRYLVYMPT
                     VNHVGVSRRIARDEERARLKELIMRMRKPGAGYIVRTVSEDIQEDEFRSDIDFLELLW
                     ENILKKKERLTAPALLHTDLDLIFRTVRDLFTNRVDRLIIDSKSEYERIRDFVTTYLP
                     HLSQRVELYDQNEPIFDHFEIEMEISKALGRKVWLKSGGYIVIEHTEALTVIDVNTGR
                     YVGKRNQEETILKTNLEAVKEIAYQLRLRNIGGIIIIDFIDMDRERNRGKVYGALQEA
                     MALDRAQTNLLRISELGLVEMSRERTREDLLRVLCEPCSYCEGRSYTKSPTSVCYEIF
                     REIRRLGSSPKEKKIIIGVHPNVAALLYDEERQGVEELEKEFHKKIVIKSDSNQHIEQ
                     YDIVTL"
     gene            complement(2493..>3268)
                     /locus_tag="VNV63_04095"
     CDS             complement(2493..>3268)
                     /locus_tag="VNV63_04095"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HXC61835.1"
                     /translation="RLAVVVVLAKYFSTHQRPGGFGFSQLLIPFLLLLIPLVLILKEP
                     DLGTALALVFTGFAILLLAGLRSRSFAVSFFLSAMFFPFVWVFFWNSLKDYQRDRLSA
                     FLDPSSDPMGRGYHLLQSKIAIGSGGLLGKGLFSATQSQLKFLPEGHTDFIFAVFSEE
                     WGFVGVFVLLTLYLLVILLGFQIALKAKDALGTYLASGIMVMFTFYLIMNIGMTLGLL
                     PVVGFPLPLMSYGGTSLVITLASLGLLMNVKLRRFMLFY"
BASE COUNT          797 a          927 c          831 g          713 t
ORIGIN      
        1 cgcccgttga atctaccgtt tttgaaatcc ccgtcatagc taccgccgtc aggatagatc
       61 agagagccct ttccgtcgat acagttgcct ttcacacagg tctctgcaat caagattgcg
      121 ggtctaccca tcaaaaatac aatgataaga aggagtagag ctattatttt ctgtatcatt
      181 gaatccttaa ctcaactgct ttatcttttc aaggtatttt tgaatccgct tggcgttggt
      241 gccgacgtcg cttcgtccga cgcgcgagac cgagcgcacg tcaatccggc tgccgtgctc
      301 ggccggcgtg atcctgacga cgacatcgtc cttgaatccg aaccagaagg ttgtatccgt
      361 cgcctcaatg cgtccatcgg ctggattcga tgcaacgatt tcccaaccca ttgcgcgcgc
      421 agcttcaagt gcccgccgaa acgcctgatc gggtggaaca tcgagcatga gcggacccaa
      481 gttcggatat cccttatgct gctgagccgc aatctcgggc ccgccatatt cggcgggatt
      541 cgcggccccg gtgcgaagcg ggagaattga aacgaaggct ggcgggttct cggtatccgt
      601 agtaatatcg tgtatcgggg gcacgtgctt tgcagtccac atccactgca atagcacaca
      661 gacaacgaca aagccgatca caaggcctgc aagccccagc ataaaacctt gccgtgatct
      721 tccgggccgc accaagatag agccaatgag cgagaccaca gcagcggcga ggctgccaaa
      781 gccaccccat ttcatgatcg taaagccggt gcggaagtcc caccatcccc aatggctgcc
      841 gagccctgct aagattaccg caaagacagc cacgagagct aaaccaaaac cgaatatcac
      901 aatgcctgat cgcttcataa gtatggctct ataacgtcac aatgtcgtac tgctcgatgt
      961 gctggttgct gtcgctctta ataacgatct tcttgtggaa ctccttctcc agttcctcga
     1021 cgccttgccg ttcttcatca tagagaagcg cagccacgtt gggatgtacg ccgatgatga
     1081 tcttcttctc cttgggagag gagccgagcc gccggatctc ccggaagatc tcataacata
     1141 cagaagttgg ggacttggtg taactgcgcc cctcgcaata agagcagggc tcgcagagaa
     1201 cccgcaagag atcttctctg gtccgctcac gggacatttc gacgagtccc agctctgaga
     1261 tgcgcagcag attcgtctgg gcccgatcaa gcgccatcgc ttcctgaagc gcaccgtaca
     1321 ccttcccgcg attccgttca cgatccatgt caataaagtc aatgatgatg atcccgccga
     1381 tgttccgaag ccttaactgg taagcgatct ccttgaccgc ttcgagattg gtcttcaaga
     1441 tggtctcttc ctggtttctt tttccaacgt atcgcccggt gttgacatca atcaccgtga
     1501 gcgcttcggt atgttcaatc acgatatagc cgccggattt cagccagacc tttcttccca
     1561 gggccttcga gatttccatc tcgatctcga agtggtcgaa aatcggctcg ttctgatcgt
     1621 acaactcgac acgctggctg aggtggggaa gataagtggt gacaaagtct cgaatgcgct
     1681 catattctga tttggagtca atgatcaacc gatccacgcg gttagtgaag agatcccgaa
     1741 ccgtccgaaa gatcagatcc aggtccgtgt gcagcagcgc cggcgccgtg agccgttcct
     1801 tcttcttgag tatgttctcc cagaggagtt caagaaaatc tatatccgag cggaactcgt
     1861 cctcctgtat atcctcgctc acggtgcgga cgatataacc ggctccaggc ttgcgcattc
     1921 tcataatgag ttcctttaag cgcgcccgtt cttcgtcccg ggcaatccgg cgggacaccc
     1981 cgacatggtt cacggtcggc atatagacca ggtaacggcc gggcaatgag atctgggtcg
     2041 tcacccggga gcctttggtg cccatgggct ctttcgagac ctgaacgagg acctcctgac
     2101 cttcttggag aagttcttcg atcggaagcg aactggatcg ccgagaccgg ggagttgttt
     2161 cagtttctgt aagggactgg gtttcttcct tctcttccga tccgtccagt gtgggctcct
     2221 ccacaggtcc cgggtattca tccggtgcgc ctgtgatgtc cgaggcgtgg agaaaggcgg
     2281 ccttttcctg accgatatcc acaaaggctg cctgcatccc gggcaggacc tttgaggcct
     2341 ttcctttgta gacattgccc acaatccctt gctctttcct gcgatcaata tacaactcgg
     2401 tcaccacccg gttttcgagc agggcgaccc gcgtctcttc ccgcgtctcg ttaatcacaa
     2461 tctcaattgc catcgatacc tcacattacg tttcaataga ataacataaa acgcctgagt
     2521 tttacattca tgaggagacc cagagaggcc agtgtgatga cgagcgaggt ccccccatag
     2581 ctcatcagcg gaagaggaaa ccccaccacc ggcaaaagac cgagcgtcat cccgatattc
     2641 atgatgagat aaaaagtgaa catgaccatg atcccggacg caagataagt ccccaaggca
     2701 tccttggctt tcagcgcaat ttgaaaaccc aggagaatca cgaggagata gagcgtaagg
     2761 agcacaaaca caccgacaaa gccccactcc tcggaaaaaa cagcgaagat aaaatcggta
     2821 tgtccctccg ggagaaactt gagctggctt tgggtggcac tgaatagccc ctttccaaga
     2881 agaccgcccg acccgatggc gatcttggac tgcaagagat ggtatccacg gcccatcgga
     2941 tcagaagacg gatcgagaaa ggcggacagc cggtccctct gataatcctt gagcgaattc
     3001 cagaagaaga cccagacaaa gggaaagaac atagcagaca ggaaaaacga cactgcaaag
     3061 gatcgcgatc gcaagccggc cagcagcagg atcgcaaaac cggtaaagac cagggcaagg
     3121 gcggttccca ggtcaggttc cttgaggatc agcaccaacg ggatcagcag cagcaaaaaa
     3181 gggataagga gctgtgaaaa tccaaatccg ccgggtctct gatgcgttga gaaatacttg
     3241 gccagcacca ccaccacggc gagtcgtg
//
LOCUS       DATMKH010000336         2055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3665797, whole genome shotgun sequence.
ACCESSION   DATMKH010000336 DATMKH010000000
VERSION     DATMKH010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3665797"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="VNV63_04100"
     CDS             complement(<1..485)
                     /locus_tag="VNV63_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014733970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HXC61836.1"
                     /translation="MHAVEIYDTTLRDGAQAEDVSFSVEDKLRIVEKLDELGVHYIEG
                     GWPGANPKDVDFFKKVRHLPLKNSTVVAFGSTRKASQRVKRDPTLQALLAAGTPIITI
                     FGKSWDLHVTKALGVTLKKNLDMIYDSVAYLKSKGRRVFYDAEHFFDGFKANPDYAMK
                     TL"
     gene            complement(508..1725)
                     /locus_tag="VNV63_04105"
     CDS             complement(508..1725)
                     /locus_tag="VNV63_04105"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009382246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HXC61837.1"
                     /translation="MGLVVQKYGGTSVGDLNRIRHVAERVAKVKASGEEVVVVVSAMA
                     GETDRLVSLAHEISSNPSDREMDLLLSSGERVTSALLALTLQELGCPAQAFTGRQVGI
                     FTDSSHTKARIERISADRIREALKEGVVPVVAGFQGIDEKSDVTTLGRGGSDLTAVAL
                     AVALKADVCDIFTDVDGVYTTDPNVVPTARKLDKISYEEMMEMASLGAKVLQTRSVEF
                     AVKYQIPVRVRSSFNEGLGTLVTKEDRDMEQVVVSGVTYDKKQAKITITGVPDMPGIA
                     ARLFGSVSEANIVVDMILQNVSQEGLTDISFTVPKADSKKAFELSRRIAKEIGAKDVQ
                     IKDDIATVSIVGVGMRSHSGVAAKMFSVLAKERINIMMISTSEIKISCVMDAKYTELA
                     VRTLHEAFELGKA"
     gene            complement(1728..>2055)
                     /locus_tag="VNV63_04110"
     CDS             complement(1728..>2055)
                     /locus_tag="VNV63_04110"
                     /inference="COORDINATES: protein motif:HMM:NF013811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HXC61838.1"
                     /translation="VEAPDEASHIGDLAAKIKSLEDFDQLTIGTLLKGMDKFGEYRIL
                     LLPDHYNPVALKTHTSEPVPYAFYEKSKTSKIKKSGKGYNEAAAQSTGIYYEEGIQLI
                     DHFMKG"
BASE COUNT          437 a          623 c          506 g          489 t
ORIGIN      
        1 agggtcttca tcgcataatc cgggttggcc ttaaaaccat cgaagaaatg ctcggcatcg
       61 tagaaaacgc gccggccctt ggatttcaga taagccaccg agtcgtaaat catatcgagg
      121 tttttcttta gcgtcacgcc caaggccttg gtcacatgaa ggtcccagct ttttccaaag
      181 atcgtaataa tcggagttcc ggccgccagc agggcctgaa gggtcgggtc gcgcttcacg
      241 cgttggcttg ctttccgggt ggagccgaag gccacaaccg tcgagttttt gagcgggaga
      301 tgccggacct ttttgaaaaa atcaacatcc ttgggattgg ccccgggcca gccgccctcg
      361 atataatgga cccccagttc gtccagcttc tcgacaatcc ggagcttgtc ctcaaccgaa
      421 aaagaaacat cctcggcctg agcaccgtcc cttaatgtcg tgtcgtagat ttcaaccgcg
      481 tgcatcgtga tttccttgtc ccgtctccta tgccttgcct aactcaaatg cctcgtgcag
      541 cgttcttacc gcaagctcgg tgtacttcgc atccatcaca caggagatct tgatctcgct
      601 cgtcgagatc atcataatgt tgatcctctc cttcgccagg acagaaaaca tcttggccgc
      661 gactccggaa tgggaccgca tcccgacacc cacgatcgag accgtggcga tgtcgtcttt
      721 gatctggaca tccttggcgc cgatctcttt cgcaatacgc cgggaaagct cgaaggcctt
      781 cttagagtcc gctttgggaa cggtaaacga gatgtcggtg aggccttcct ggctcacatt
      841 ctggaggatc atgtccacaa cgatattcgc ctcgctcacc gacccaaaca gcctggccgc
      901 gatccccggc atatccggca cgccggttat cgtgatcttg gcctgcttct tatcataggt
      961 cacacctgag acgactacct gctccatgtc acgatcctcc ttcgttacca aggtgcccaa
     1021 tccctcattg aagctggaac gcacgcgaac cgggatctga tatttcaccg caaactcaac
     1081 cgaacgggtc tgaagcacct tcgcccccaa actggccatt tccatcatct cttcataaga
     1141 gatcttgtcc agtttcctcg ccgtgggtac gacattggga tcggtggtgt aaacaccgtc
     1201 cacatcggta aagatgtcac agacgtcggc ctttaaggcc accgccagcg ctaccgcggt
     1261 gaggtccgaa cctccccgtc ccagcgttgt aacatccgac ttttcgtcga tcccctgaaa
     1321 accggccacg accggtacta cgccttcttt caaggcctcc cgtatccggt cggccgagat
     1381 ccgttcgatc cgggccttgg tatgagagct gtccgtgaag attcctacct gccgaccggt
     1441 gaaggcctgg gctgggcagc cgagctcttg caaggttaaa gcaaggagcg cgctggttac
     1501 ccgctctcct gaagaaagga gaagatccat ttcccggtcg gatggattgg aggagatttc
     1561 atgggccaag ctcaccagcc ggtcggtctc tccggccatg gctgagacca ccaccacaac
     1621 ctcctcgccc gaagccttga ccttggccac acgctccgcg acgtgtctga tccggttcaa
     1681 gtcgcctacc gaagtaccgc cgtatttttg aaccacaaga cccattgcta gcccttcatg
     1741 aaatgatcaa tcaactggat gccttcctcg taatagatgc cggtggactg agccgccgct
     1801 tcgttatagc ctttgcctga tttcttaatc ttgctggtct tggacttttc atagaacgcg
     1861 tacggcaccg gctccgatgt gtgagtcttc agggccaccg gattataatg atcgggaagc
     1921 aggaggatgc ggtattcccc aaatttgtcc atgcctttta aaagtgttcc gatcgtcaat
     1981 tgatcaaagt cctccagaga tttgatcttc gccgcaagat ctccgatgtg gctcgcctca
     2041 tccggtgcct cgaca
//
LOCUS       DATMKH010000337         1148 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1757083, whole genome shotgun sequence.
ACCESSION   DATMKH010000337 DATMKH010000000
VERSION     DATMKH010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1757083"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(790..>1148)
                     /locus_tag="VNV63_04115"
     CDS             complement(790..>1148)
                     /locus_tag="VNV63_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61839.1"
                     /translation="THVVDLKYETRVGEKVWPVSWADEFTDYARWKASGQPEGYVFGT
                     DWSLAFPGISVLLASSEAQDWSNRLRRPMHSASIETDRFCISLVFSETRHRKVSDEVG
                     IVRQVLIPLPTPGRLQ"
BASE COUNT          296 a          285 c          319 g          248 t
ORIGIN      
        1 gttgtcgaaa ccaggcgatc acaactcgcg attcgggtct ggccggtctg ggttccggcg
       61 gagtcacggg caaaccggat gttgagaaac ggcagctcgt tgcggtgtta cgatgatttt
      121 gccaggtgaa acggaagctg gcactgcgga aaagaggtga cacggaaaag agatgacaga
      181 caggacgatc acctatgagc tatgaatatg taagtaatcg tcctgtctgt catctctttt
      241 ccgttcagtt ttgtctgcag aacgaatgat tactcgatac ccattgggta atccatattg
      301 gcagagaaga atctcggttg attgaggtgt gatagctgcc tcgataacgg gttgcgcgaa
      361 gtagcgtctc acccggggac caggaacgaa gctaataccc tcgtggctga tttgaagtcg
      421 ggccatcgca ctccgaggtg gaaatgctag ccgcaacaag aacgatgccg ggattagcaa
      481 aattgcataa agtagccacc cgacccagga gtccatgaaa gcatgagcga tgcggtcaaa
      541 tccgaccttt gtggggaacc gacaggcgag gagcaagaac ggcagtgcac ccgcaagcgc
      601 tatgatcctc cgtatgaaag agggggagat cacgaaggca attgaatccg cgcgttcttc
      661 tgagatcgcc atccgacaga agtatatcgg gaccgccgtc ggtgtggatg gtcctccgtg
      721 ccaaaggaag ttacctataa tgcgacttgg caggagtttc gggattactc ttgttccccg
      781 tttgccctcc tattgaagtc tcccgggagt gggtagaggg atgagcactt gtcgaactat
      841 tccaacttca tcagatactt ttcgatggcg ggtttcactg aagaccagcg aaatacaaaa
      901 acggtcagtc tcaatgctgg ccgaatgcat aggacgccga agacgattcg accaatcctg
      961 tgcctcggaa ctggcgagca gaacgctgat tccgggaaac gcaagagacc aatccgtgcc
     1021 gaacacatag ccttcaggct gaccggaagc cttccatctg gcgtagtccg taaattcatc
     1081 tgcccacgaa accggccaga ctttttcacc tacccgggtt tcatacttga gatccaccac
     1141 atgcgtaa
//
LOCUS       DATMKH010000338         1253 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2395301, whole genome shotgun sequence.
ACCESSION   DATMKH010000338 DATMKH010000000
VERSION     DATMKH010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2395301"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..992
                     /locus_tag="VNV63_04120"
     CDS             <1..992
                     /locus_tag="VNV63_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61840.1"
                     /translation="CASVVTDVLVLRGKMQRRLRGQSFAARGDFSSEPSVNVLLKASG
                     ILKHLGVRGAALPPDVQERLKLCPLHAGKPSPPSKSSEGEIAATDLTRFFDNCLSTEN
                     YALKLEWKSKLISLITEVLDNSEQHANRDRYWYTIGHFNHLENEPGGDCHIVVFNFGD
                     SIYDSLKRRDTSESLKKQIADLASRHRSRGFLGFQKPKWQEETLWTLYALQEGVSRFA
                     DTPEGIDRGNGTVRMIQFFTELAGEQPKMVVVSGQTHILFDGTYVLRDRLMSGGERRK
                     IIAFNAENDLEKPPDERFVRHLPLKFPGTLVSLRFILHRDRLKLVQELVDERN"
     gene            979..>1253
                     /locus_tag="VNV63_04125"
     CDS             979..>1253
                     /locus_tag="VNV63_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61841.1"
                     /translation="MNGIKRIDLGEARTSKEARVFSGRERGKYWRQYFKLDQLDQDDS
                     TVEVVIPEDILSVNLSFFLSLFGESVRHLGSVDFKAKYHFVCDPSLLP"
BASE COUNT          312 a          323 c          335 g          283 t
ORIGIN      
        1 tttgcgcctc cgttgtgacg gacgtgctgg tgcttagagg aaagatgcag cgccgactaa
       61 ggggtcagag cttcgccgcc cgtggggact tctcttccga accaagcgtt aacgtcctac
      121 tcaaggcaag cggtattctt aagcacttgg gagtacgtgg cgcggcactc cctccagatg
      181 tacaggagag gctcaaactg tgccctctcc acgccggtaa gccctccccg ccttccaagt
      241 ccagtgaagg cgagatcgcg gctacggatc taacaagatt ctttgacaac tgcttgagca
      301 cggaaaacta tgcactcaag ctggaatgga aaagcaagtt aatctctctc attacggagg
      361 tgcttgacaa ctctgagcag cacgctaatc gcgaccgata ttggtacacg atcgggcact
      421 tcaatcatct cgagaatgaa ccgggcggag attgccatat cgtagtattt aacttcgggg
      481 attcgattta cgactccctg aaacgcaggg acacctcgga atcgctcaag aagcaaatcg
      541 ccgatttggc atctcggcac cgttctcgcg gtttcctcgg gtttcaaaag cccaaatggc
      601 aggaggaaac tctatggacc ctttacgctc tccaggaggg tgtgagccga ttcgccgaca
      661 cgcccgaggg tatcgaccgc ggcaacggaa cggtgaggat gatccaattc ttcacggagc
      721 tcgcagggga acaacccaag atggtcgtgg tatccggtca aacccacatc cttttcgatg
      781 ggacctacgt cctaagggat agattgatgt ccggaggaga acggcgtaaa attattgcat
      841 tcaatgcgga gaatgacctt gagaaaccgc ccgacgaaag attcgttcgt catttgcccc
      901 ttaaatttcc aggaaccctc gtgagccttc gcttcatctt acatagggac cgtctgaagc
      961 ttgtccagga gttagttgat gaacggaatt aagagaatcg atttgggcga agctcgaacc
     1021 tcgaaagagg caagagtatt ttcaggcagg gagcgaggca agtactggcg gcagtacttc
     1081 aaactggatc agctggacca agacgattcg acggtcgagg tggtgatacc agaggacatt
     1141 ctctcagtca atctgtcttt tttcctaagc cttttcggcg agagcgtccg acatttgggg
     1201 agcgtggatt ttaaggcaaa ataccatttc gtctgcgacc catccctact acc
//
LOCUS       DATMKH010000339         1269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2082213, whole genome shotgun sequence.
ACCESSION   DATMKH010000339 DATMKH010000000
VERSION     DATMKH010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2082213"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..991
                     /locus_tag="VNV63_04130"
     CDS             <1..991
                     /locus_tag="VNV63_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61842.1"
                     /translation="LAFAILAWKGLFVPDQENQSNRRFRVLLSGSHLLLGGLLAFVFI
                     RFKMHFFLPDTALAKSDGIPNWYVTLYVTGKIFAGALSFGAGMFLFWLLTAFMALRAG
                     RFSISTLFANLAFPALLALAASRGQQIQGARYFVWTLFFSILWNIFELSSVPPDHRMQ
                     AQNNTLAFCFLVLLLLALPFESRMLYPMLRDRAGLLTQFEESHFETLQGKRGVAVDIG
                     YIGYFSRADICDLAGLVNGRDRARQTSQERLVGCFASRPDFIFFNLGDIKYAANRLPA
                     KLPINDWQICSRYDFKTVIQNDPHYLIVPRSVAPEVCKNVANTIPYDIPPLLR"
BASE COUNT          247 a          382 c          287 g          353 t
ORIGIN      
        1 gttggccttc gcgatcctcg cctggaaggg tctcttcgtt ccagaccagg agaaccaatc
       61 gaatcgccgc ttccgtgtcc tcctcagcgg cagtcatctt ttgctgggcg gcctccttgc
      121 cttcgtgttt atccgattca agatgcactt ctttcttcct gataccgcgc tggcaaaatc
      181 cgatggcata ccgaattggt acgttaccct ttacgtgacg gggaagattt ttgcaggagc
      241 cctttccttc ggagccggaa tgtttctttt ctggctcttg accgccttca tggcgctgcg
      301 tgccggccgc ttttcgatat cgacactatt cgccaacctg gcttttcccg ccctgcttgc
      361 cctggccgcg tcacggggcc agcagatcca gggtgcccgt tatttcgttt ggaccctctt
      421 cttctctatc ctttggaaca tcttcgaact gagcagcgtc ccgcccgatc atcggatgca
      481 ggctcaaaac aacacactgg ccttttgttt cctggtgctg ctcctgcttg cgctcccatt
      541 tgaatccaga atgttgtacc ccatgctgag agatcgtgct ggcctcctga cacagttcga
      601 agaaagtcac ttcgaaaccc tacagggcaa acgtggagtc gccgtcgaca ttggctatat
      661 aggctatttt tctcgcgctg atatctgtga cctggccggc ctggtgaatg gcagagacag
      721 agcgcgccag acaagccagg aacgtctcgt aggctgcttc gccagtcgtc ccgactttat
      781 cttcttcaac ttgggggaca ttaaatatgc ggccaaccgt cttcccgcca aacttcccat
      841 caatgactgg cagatttgct cccgctatga cttcaaaact gtaatccaga acgatcctca
      901 ctacctcatt gtgccgcgat ctgtcgcccc ggaagtctgt aagaatgtgg caaatactat
      961 cccctatgac attccgcccc tgctccgtta gctgagacga tctcaagact atagttgata
     1021 tgttcttcta tatcggaatc tgcattgttg ctgctgtcat aggcgctgct ccaccagaag
     1081 cacatgaaac ctgaaccagg tagtcttgcc gtaatttgtc ccctctcccg ttcgttacaa
     1141 cactttcaat cacaagaatg ggccggtccg ttctacgagg aaatctttga ccgcaacaaa
     1201 tgttcgattc tatcggcctg agctcgatgt cctccgcttc ttcgccttcc tgctcgtctt
     1261 tctttatca
//
LOCUS       DATMKH010000340         1484 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3043789, whole genome shotgun sequence.
ACCESSION   DATMKH010000340 DATMKH010000000
VERSION     DATMKH010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1484
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3043789"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="VNV63_04135"
     CDS             complement(<1..431)
                     /locus_tag="VNV63_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61843.1"
                     /translation="MKRNHITAITLMLGTFLFALAFHGCNFESGKRKPMMTLFIGLDE
                     SGSFTNSRYYEDALTFTANYIYGHLHELGGLAKPRELFVASVGGRALDDPKTFHPILD
                     FEGKSVSEIVEDLKRWFPPNDKVTDFNSFFEKSAQLAKERNL"
     gene            complement(428..>1484)
                     /locus_tag="VNV63_04140"
     CDS             complement(428..>1484)
                     /locus_tag="VNV63_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61844.1"
                     /translation="CTQLSIYTVPFRSGRIHFFTLILLSWWDAARAVWLYWTGLIRFL
                     LVGMGWLFGIARLFLKLMVEALRQVLVLPFSVTGRMTKSYFKPGVPWIAFLMLLFWCL
                     MEALIFTYTLLPTVTEVLSDLVGTESSPYMGTILYLFLFMLIMGSFACIQALLEAVKK
                     GEIKFLVQMIMVELFVMFFEVMFLYRELVDAITPWIAQQTGESFRMGIISTLTLATFG
                     WIGIRGMTWFLFGQFGTPPLLAFISRRPLVQDGPAEQVAREEASPAWWKKPIEDFKGE
                     IVWLHEKSNEFLEYLALPAMQVMAAALNFSLVLVASRPIFSIPFKNLREVMDTRNILA
                     AMQLRLKPALEPKKVQS"
BASE COUNT          390 a          387 c          384 g          323 t
ORIGIN      
        1 agattccgtt cctttgccaa ttgggcagat ttttcgaaga aggaattaaa gtccgtgacc
       61 ttatcgtttg gtggaaacca gcgcttcagg tcttccacga tctcggagac gctttttccc
      121 tcaaagtcga ggatgggatg aaatgtcttg ggatcatcca gggctctacc accgacggat
      181 gccacaaaga gctcgcgggg tttcgccagt cctcccaatt cgtggagatg gccgtagata
      241 taatttgccg tgaacgtcaa ggcatcctca tagtaacgac tgttggtaaa agagccgctt
      301 tcatccaagc cgatgaaaag ggtcatcatc ggtttgcgtt tacctgattc gaaattgcaa
      361 ccgtggaaag ccaatgcgaa gagaaaggtc ccgagcatca aagtaattgc cgtgatgtgg
      421 tttcttttca tgactgcacc tttttgggtt caagcgccgg tttgagccgg agttgcatcg
      481 ctgcaaggat attccgggtg tccatcactt cacgaagatt cttgaacggg atgctgaaaa
      541 tagggcgtga agcaaccagg accagactaa aattcaatgc cgcggccatg acctgcatag
      601 cggggagggc aaggtattcc agaaattcgt tgcttttctc gtggagccac acgatttctc
      661 ccttaaagtc ttcgatcggc ttcttccacc aggcaggaga tgcttcctcc cgagcaactt
      721 gttcagccgg tccatcttgc accagaggcc gtcttgaaat aaaggccaac agaggcggtg
      781 ttccaaactg gccaaacagg aaccaggtca tgcctcggat accgatccat ccgaaggtgg
      841 cgagcgtcag cgtggagatg attcccatac gaaaactctc tcctgtctgc tgagcgatcc
      901 acggggtgat cgcatccacg agttcccgat acaggaacat cacttcaaag aacatcacaa
      961 acaactccac catgatcatc tggacgagga atttgatctc ccctttcttg accgcctcca
     1021 ggagcgcctg tatgcaggcg aagcttccca taatgagcat gaacagaaag aggtaaagga
     1081 tcgtgcccat ataaggcgac gattccgttc ccacgaggtc gcttaaaacc tccgtgacgg
     1141 ttggcaagag tgtgtaagtg aagatgagcg cctccatgag acaccagaat agaagcatca
     1201 aaaaggcgat ccaggggacc cccggcttaa aataactttt ggtcattctg ccggtgaccg
     1261 agaagggcaa gactaacacc tggcggagcg cctccaccat cagtttcagg aacaacctcg
     1321 cgatgccgaa gagccaaccc atcccgacca gaagaaagcg gatgagaccg gtccagtaga
     1381 gccacaccgc tctggccgcg tcccaccatg ataacagtat cagcgtgaag aaatggattc
     1441 tgcctgaccg gaagggaacc gtgtagatgg acagctgtgt gcac
//
LOCUS       DATMKH010000341         2848 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_801651,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000341 DATMKH010000000
VERSION     DATMKH010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_801651"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /locus_tag="VNV63_04145"
     CDS             <1..520
                     /locus_tag="VNV63_04145"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HXC61845.1"
                     /translation="ALAKEIRKEVEALIDAGAKIIQVDEPALSVRPEEIPFAVEAMDI
                     VTRDLPAYFITHICYGAFEFIYPKMLELPVENFDLETSNSGLDLLNLFARNPFTKDFS
                     FGVVDVHTHQIESPELVQDRIKKALNLLPKEAIWVDPDCGLKTRTVDEAKEKLRTVVI
                     GARAIRQELGVA"
     gene            517..1593
                     /locus_tag="VNV63_04150"
     CDS             517..1593
                     /locus_tag="VNV63_04150"
                     /inference="COORDINATES: protein motif:HMM:NF016338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61846.1"
                     /translation="MREPLFDEVANFIESPQSGRFEFLALRVFEHQFEHNLPYRKFCL
                     KRGKTPDKIHSYREIPAVPTAVFKEMDLSCGPSEMIFLTSGTTQGAAKRGRHLIQDLT
                     LYHASLKPNFAAHLLPEGQRLRILVLFASPKLMPQSSLSHMFETVIDTFGSEGSDYFF
                     SDQGLDSFGLVKALQASEQRGEPVCLLGVSFAFVQFLNYCDSERLRFRLPASSRLMDT
                     GGYKGRMREVPRGELYYRLGKALGIPTSFMINEYGMTEMCSQFYDNVLADRFRGVQRS
                     RHKVMPRWVRTRILNPETLEELPDGVTGILQHYDLANAGSVMALQTEDLGYRIENEFE
                     IIGRAKDAEARGCSLTMEEFLRIQ"
     gene            1590..>2848
                     /locus_tag="VNV63_04155"
     CDS             1590..>2848
                     /locus_tag="VNV63_04155"
                     /inference="COORDINATES: protein motif:HMM:NF017686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0008218 - bioluminescence [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HXC61847.1"
                     /translation="MKIRGYFLPPHIKVKDYTTLTFDRVEIEVPVLTPDLIWVITEHL
                     LAFHDRVLMKRPVHSIVKAFGSAIRQWQKPSEPRQLALAALPAITGFSPEMIVRILEK
                     PWAEYRSDKINVFLKNNIYNYKILDEFTMRPSMQSRAFGPRLTTHILAGNIPGAGIPD
                     LIMASVIKSANLVKTSGGEPLLPVLFAESLAEIDSELAQGLAVVWWRGGDQAIENAAF
                     SRSDCVIASGSDETIKGIQQRVKSRFIGYGHRLSFGLIGKEVLKNARTIAKRAALDVA
                     MYDQQGCLSPHLFYVEAGGTVTPKEFAGLLADELEALSIKLPRGKISTEAASTLRQLK
                     DLSEIREAAGNEVAVYSRKDLDWLAIYEADPFFVPSPLYRAIRVKPLKDFSLLPSLLQ
                     SWTPYLQAAGVAVGKDRRKKVAESLGRL"
BASE COUNT          658 a          712 c          781 g          697 t
ORIGIN      
        1 ggcgttggcg aaagagatcc ggaaagaggt ggaggcgctg atcgacgcag gcgccaagat
       61 tatccaggtg gacgagccgg ccctctccgt gaggccggag gaaattcctt ttgcggttga
      121 agcgatggat atcgtaaccc gcgacctgcc ggcctatttc attacgcata tctgctacgg
      181 cgcgtttgag ttcatctatc ccaagatgct ggagttaccg gtagagaatt ttgatctcga
      241 gacgtccaac agcgggctgg atcttttaaa tcttttcgcc agaaatccct ttacgaaaga
      301 tttctcattc ggggtggtgg atgtccatac gcatcagata gaatcccctg agcttgtgca
      361 ggaccggatc aagaaggcgc tgaacctttt gccgaaagaa gcgatttggg tggatccgga
      421 ctgtggactc aaaacaagga ccgtggacga ggcgaaggag aaactcagaa ccgtggtgat
      481 cggcgcccgt gcgatccggc aggagttagg tgtggcgtga gggaaccgct gttcgatgag
      541 gtcgcaaact tcatcgaatc cccgcaaagc ggacggttcg aattcctagc cttgcgtgtt
      601 tttgagcatc aattcgagca caatctcccg tacagaaaat tctgtctgaa acgcgggaag
      661 actcccgata agatccattc ctatagagaa atcccggccg ttccgaccgc tgttttcaaa
      721 gagatggatc tgtcatgcgg tccttccgag atgattttcc tcaccagcgg cacaacccaa
      781 ggcgctgcaa aacgcgggcg acatctgatc caggatctta cgctttatca cgcatcgctc
      841 aagcccaact ttgcagccca tctccttccg gaaggccagc gcctcagaat actggttcta
      901 ttcgcctctc caaagttaat gccgcaatct tcgctgagcc acatgtttga gacggttatt
      961 gacacttttg gctccgaggg gagcgactat tttttttctg atcagggtct cgactcattc
     1021 ggcctggtca aggcccttca ggcctcggag cagcgtggcg agccggtctg ccttttaggg
     1081 gtttcttttg cttttgtgca gtttctgaat tattgtgatt cagagcgtct ccgctttcgg
     1141 ctgcccgcaa gcagcaggct catggacacg gggggttata aaggcaggat gagggaagtt
     1201 cccagaggag aattgtacta taggctggga aaagcgctgg gaatcccgac aagctttatg
     1261 atcaatgagt acggaatgac tgagatgtgt tcgcagttct atgataatgt actggcggat
     1321 cgatttcgcg gtgttcagcg gtctcgccat aaagtcatgc cgcgctgggt tcgcacccgg
     1381 atcttgaatc cggaaaccct cgaagaacta cctgacggcg ttacgggcat ccttcaacat
     1441 tatgatttgg ccaacgcggg ttcggtcatg gcgctccaga ccgaagattt gggttatcgg
     1501 atcgaaaatg aatttgagat catcggacgt gcgaaggacg ccgaggcacg cggctgttct
     1561 ctcaccatgg aagagtttct acgcatccaa tgaagatccg gggatatttc cttccgccac
     1621 atatcaaagt caaggactac acaacattga cctttgatcg ggttgaaatc gaggtgcctg
     1681 ttctcacgcc cgacttgatc tgggtgatca ccgaacatct tcttgccttc catgaccgcg
     1741 tgctgatgaa gagaccggtt cattcgatag tgaaggcttt cggttccgcg atccggcagt
     1801 ggcaaaagcc gtctgaaccc cgccaattag cgctcgccgc gcttccggca atcacaggtt
     1861 tttcacccga aatgatcgta cgaatcttag agaagccttg ggccgaatat cgatcggata
     1921 aaataaatgt ttttcttaaa aataatattt ataactataa gattttagat gaatttacga
     1981 tgcggccatc tatgcaaagc cgtgccttcg gtcctcgact cacgacccat atactggctg
     2041 gaaacatccc cggcgcaggt atcccggatt tgatcatggc gtccgtgatt aagtctgcaa
     2101 atctggtcaa gacctccggc ggggaaccgt tgctgccggt tctgtttgcg gagtctctcg
     2161 ctgagataga ttccgagctc gcccaagggt tggcggtcgt ctggtggaga ggtggtgatc
     2221 aggcgatcga gaatgccgca ttttcgcgct ccgactgcgt gatcgcctcc ggcagcgatg
     2281 aaacgattaa ggggattcaa caacgtgtga aatcccgttt tatcggctac ggccatcgat
     2341 tgagcttcgg cttaatcggt aaagaggtct tgaaaaatgc caggaccatt gccaagcggg
     2401 ccgcattaga tgtggcgatg tatgatcagc aaggctgtct ctctccgcat cttttttatg
     2461 ttgaagcggg cgggaccgtg acgcctaaag agtttgccgg attacttgca gatgagcttg
     2521 aagcgctttc aatcaaactc ccgcgcggaa agatttcaac agaagcggcc tcgacgctca
     2581 ggcaactgaa agacctctcc gagataaggg aagcggccgg caatgaggtc gcggtataca
     2641 gccggaaaga tttggactgg ctggcgatct acgaagccga tccgtttttt gtgccctcgc
     2701 cgctttaccg ggcaatccgt gtaaaacctc tgaaggactt ttctcttctt ccttctttac
     2761 tacaatcatg gactccctat ctccaagcgg cgggcgtagc ggtgggaaaa gatcgtcgga
     2821 agaaagttgc ggagtcgttg gggcgtct
//
LOCUS       DATMKH010000342         1094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2082672, whole genome shotgun sequence.
ACCESSION   DATMKH010000342 DATMKH010000000
VERSION     DATMKH010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2082672"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..>1094)
                     /locus_tag="VNV63_04160"
     CDS             complement(39..>1094)
                     /locus_tag="VNV63_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61848.1"
                     /translation="GGEPFAWFDGISAWPSMMIILLAAFLSSHFILKTQLNLRQNADT
                     LAEDFGLERSTKQQASSFGWEVTPAITEITGASPPTSGRPGKSEERVDIQALWQRYLC
                     RGQFRNRVKRAAPMTVLYIAALFTLQPILGGFPRPPIRGESLYFFYLIFFTICLFLFL
                     TFFVIDAIFLHEGFLLQLEKNETCWPDATFHRFEYSITPSRPLNERDLADYWDILLIS
                     KRTEAVGRLIYYPFIILSLLIVARLNCFDNWSWSPVVILILSMHFSLALYAAWRLPKA
                     AREYRDKVLERLQRRRRQALMLAQRTPEAIDTLIEEVQSTHQGAFSYLWEQPAIRALL
                     LPSGGIGLATLLQFLPH"
BASE COUNT          287 a          271 c          330 g          206 t
ORIGIN      
        1 ttgcgcggaa aaagcgcatt ttcgggactg accctagatt agtggggcaa gaattgcagc
       61 aacgtggcca ggccaatccc gccggatggc aacaagagcg cccggatggc cggctgttcc
      121 cacaagtaag agaaggcgcc ctgatgggtg gactgcacct cctcgatcaa ggtatcgatg
      181 gcctccggcg tcctttgggc cagcatgagc gcttgccgcc tgcgtcgctg cagacgttct
      241 aagaccttgt cccgatactc cctggcggct ttgggaagtc gccaggctgc gtaaagagcg
      301 agggagaagt gcatggagag gatcagaatg accaccggcg accaagacca attatcaaag
      361 cagttcagcc gggccacaat caagagcgac aaaatgataa agggatagta gatcaaacgc
      421 ccgactgctt cagtgcgctt tgaaatcagc aggatgtccc agtaatccgc aagatctctc
      481 tcgttcagag ggcgacttgg tgtgatggaa tattcgaatc ggtgaaatgt agcatccggc
      541 cagcatgtct cattcttttc gagttggagg agaaagcctt cgtgtaagaa aatggcgtcg
      601 atcacgaaga aggtaaggaa gaggaagagg cagatggtga agaagatcaa ataaaaaaaa
      661 tagagagact cgcctctgat cggggggcgg ggaaaaccgc ctaagatagg ctggagcgtg
      721 aagagggccg cgatatagag gaccgtcatc ggtgcggcac gcttgacccg gttccggaat
      781 tggcctcggc aaagataacg ttgccacaag gcttggatat cgaccctctc ctccgacttg
      841 cctggccgtc cggaagtggg aggggaggcg ccggtgattt ccgttatcgc cggagtgacc
      901 tcccaaccaa aagaggaagc ttgctgcttg gtagaacgtt ccaatccaaa atcctccgcc
      961 aatgtgtccg cattttggcg aaggttgagc tgggtcttca aaatgaagtg ggaggacaga
     1021 aacgccgcca agagaataat catcatggac ggccaggcac tgattccatc gaaccaagcg
     1081 aacggctcgc cgcc
//
LOCUS       DATMKH010000343         1224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_162547,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000343 DATMKH010000000
VERSION     DATMKH010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1224
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162547"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..>1224)
                     /locus_tag="VNV63_04165"
     CDS             complement(50..>1224)
                     /locus_tag="VNV63_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61849.1"
                     /translation="GSIHTWSLEQASDLVVHTDRNLLPRKELPRLVWVPDGRLIFGAY
                     NPSEQGSDGLWEMPMDNQTGKSSGNPVQITNWHGDQAASPSASLDGRRLVVSKRHTKA
                     DIFLSELKQKGRRIEVAKNLTLGDSFENPNAWLADSRAMLISSSRTGRGQIYRLYLDG
                     RNPEAIAPGPDIQDDAEISPDGAWIMYWSIQHDEKGNLKSLSATRVSSAGGTPERIFE
                     SQPDQTTYFHCPSRPGSPCVLSLWEKDQLSFYEFDAVKGQGKELARTHLEKPKILAWS
                     ISPSGSQIAMTSRDSLKEQIRVVNLNGVGEKNIPLPKGSLSWDTGWTPDGAAILLSGF
                     ITGAGIARVELDGKTTVLLQGVKSQFFYHPIESPDGRYLAYAQQGWNSNVWLLENF"
BASE COUNT          270 a          355 c          303 g          296 t
ORIGIN      
        1 ctgaccgaag gcggattcca cttgatgaga ccggatgaga tcgtacgggt cagaaattct
       61 cgagcagcca aacgtttgag ttccatccct gctgggcgta agcgagataa cgcccgtcgg
      121 gtgactcgat cggatggtag aaaaactgac tcttcacacc ctgaagcagg acggtagttt
      181 tgccgtccaa ctccaccctt gctatccctg ccccagtaat aaaaccggaa aggaggattg
      241 cagcgccatc gggcgtccag cccgtatccc aagacagtga ccctttcggc agcgggatat
      301 tcttttcgcc gaccccattc agattaacaa cgcgaatctg ctctttaagc gaatctcggc
      361 tggtcattgc aatttgagaa cccgacggag agatgctcca cgccagaatt ttcggttttt
      421 ccagatgggt tctcgccaat tcctttcctt gccctttcac cgcatcgaat tcataaaagc
      481 tcaactgatc cttctcccag aggctcagca cgcacggact accgggacgc gatgggcaat
      541 gaaaatacgt agtttgatcg ggctggcttt caaatatccg ttccggcgtc ccgcccgcgg
      601 aagatactct cgtcgcgctt aaggacttca gatttccttt ctcatcgtgc tggatggacc
      661 aatacataat ccacgcacca tcaggactga tttcggcatc gtcctgaatg tcgggccccg
      721 gagcaatcgc ttccggattc ctgccatcga ggtacagacg gtagatctgt cctctcccag
      781 ttcgcgagga agaaatcaac attgcgcgac tgtccgccaa ccaagcattc ggattctcaa
      841 agctatctcc gagggtgagg ttcttcgcta cttcaatccg ccgtcctttt tgtttgagtt
      901 cactcaaaaa gatatcggct tttgtgtgcc tcttactaac caccagccgg cggccatcca
      961 aactcgcgct cggagacgcc gcttggtccc catgccagtt tgtgatttgc acagggtttc
     1021 cggaggactt accagtttgg ttatccatcg gcatctccca caggccatcg cttccctgtt
     1081 ccgagggatt gtatgctccg aagataaggc gtccgtccgg aacccatacg agcctgggca
     1141 actcttttcg cgggaggaga ttgcggtcgg tgtgtacgac caagtccgaa gcttgctcca
     1201 aggaccaggt gtgaatactg cctg
//
LOCUS       DATMKH010000344         3709 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3668486, whole genome shotgun sequence.
ACCESSION   DATMKH010000344 DATMKH010000000
VERSION     DATMKH010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3709
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3668486"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            299..793
                     /locus_tag="VNV63_04170"
     CDS             299..793
                     /locus_tag="VNV63_04170"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HXC61850.1"
                     /translation="MNQNPPLTGNLNEVYLSEILGHLQSIQATGTLTIEQDHQVKSIY
                     LKDGQIVFASSNLEQDRLGETLLKAGKVSKQQYEKSVEVLKNTGKRQGAVLVELGFLT
                     PKELFEGLKYQVLEILYSLFLWKKGRYSFVTGELPIRIIPIRIDLVVLISEAIKRIDE
                     DQAA"
     gene            complement(824..1054)
                     /gene="rpsR"
                     /locus_tag="VNV63_04175"
     CDS             complement(824..1054)
                     /gene="rpsR"
                     /locus_tag="VNV63_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008676141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HXC61851.1"
                     /translation="MERTRFFQRRKLCRFCTDKVRHIDYKDITLLRNYLTERGKIIPR
                     RISGTCNRHQRHLATAISRSRNIALLAFAEER"
     gene            complement(1087..1503)
                     /locus_tag="VNV63_04180"
     CDS             complement(1087..1503)
                     /locus_tag="VNV63_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HXC61852.1"
                     /translation="MTSFNKVILMGNLTKDPEIRYTPSGTPVANFRLAVNNRYKQGEE
                     FKEEVCYIDIVVFGKQAENCGQYLGKGQGVIVDGRLQERRWETEDGVKRSKHEVVAQT
                     VRFMSRRQGAPSEKSALGGEGMPEPETEAVDNDVPF"
     gene            complement(1562..1936)
                     /gene="rpsF"
                     /locus_tag="VNV63_04185"
     CDS             complement(1562..1936)
                     /gene="rpsF"
                     /locus_tag="VNV63_04185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HXC61853.1"
                     /translation="MQNYESIVIVRSTLSEEEINAVLAKIREIIEKAGGTVLQAEGWG
                     KRRLAYEIKREKKGAYLLFRFQADGKLIAQLEHQYRLDETILKFITVRLEGPLPTTSA
                     SGQAAEKKPVHEKATLEEAPSV"
     gene            complement(2060..3157)
                     /gene="ychF"
                     /locus_tag="VNV63_04190"
     CDS             complement(2060..3157)
                     /gene="ychF"
                     /locus_tag="VNV63_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HXC61854.1"
                     /translation="MGFQCGIVGLPNVGKSTLFNALTAGRAEVANYPFTTIDPNVGIV
                     EVPDERLQRLTAIYRPKKVTPTTVEFVDIAGLVKGASQGEGLGNKFLAQIREVDAIVH
                     VVRCFENPDVSHVSGSVDPVRDIDVIHTELVLADLETIEKRLQGVEKRVKAGDPKTSV
                     EMEFLRRLQSLLGRGEPIRGKDYTPEEQTHLHGYRLLTDKPILYVANVAEDELNRENR
                     HVQELEKAAKRENAAAVVISGKVEQEIAALPIGERGVFLADLGIKESGLDRLIRAGYA
                     LLGLITFFTAGEEEVRAWTVPRGAKAPEAAGRIHSDMEQGFIRAEVFRYSDLISSGSS
                     ASVREKGLLRLEGKDYIVQDGDIVYFRFQPR"
     gene            complement(3205..>3709)
                     /gene="pth"
                     /locus_tag="VNV63_04195"
     CDS             complement(3205..>3709)
                     /gene="pth"
                     /locus_tag="VNV63_04195"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HXC61855.1"
                     /translation="HNLGYRVVDALAQQHRISLRERTEKVMYGQGSIKATKVVLAKPL
                     TFMNRSGAAVRDLCKTFQISPADLIVIHDDIDLDFGCLRIKTRGGYGGHNGLKSILEI
                     LETDRFVRIKLGVGRPKGEEVAEHVLSPFTAQERPLVEEMISRAVAATELVVAEAVAQ
                     AMNQFHN"
BASE COUNT          857 a         1063 c          868 g          921 t
ORIGIN      
        1 aagaagagac ctcccagact gaggtcctgg gcgtagccga ggaagacctc attatatttc
       61 tttcccttca cctcgacgac aagaaaggga aggcgcggat accctcttct ctctgaggga
      121 tctttttcag atacaggatt ctgaggttca agtttcatgg tggcttaggt ggaaactata
      181 acaagtttca attcatcagt caaggaattt gtcagaataa accgccatca tcaaatatct
      241 catgttgaca aaataaaaag aaccatataa ctttaaagga gtgcctggag atcactggat
      301 gaatcagaac ccgccgctga ccggaaacct aaatgaagtt tatctctccg aaattttagg
      361 acatctccaa tctatccaag caaccggcac cctcacgatt gaacaggacc accaggttaa
      421 gtccatctat ctcaaggacg gccagatcgt ttttgcctct tccaatcttg aacaggaccg
      481 tttgggagaa acgcttctca aggcaggcaa ggtgtcgaag cagcagtatg agaagtcggt
      541 tgaggtgctg aagaacacag gaaagaggca gggcgcggtg ctggtggagc tcggctttct
      601 gacgcccaag gaattgtttg aaggacttaa gtatcaggta ttggaaattc tctacagcct
      661 tttcctatgg aagaaaggtc gttattcatt tgtcacaggt gaactgccca tacggatcat
      721 cccaatccgt attgaccttg tcgtgcttat ttccgaagcg atcaaacgga ttgatgaaga
      781 ccaggccgct tagctgaaaa accatctttc tttaggctgt agcctaacgt tcctctgcaa
      841 aagcaagcaa cgcaatattc cgcgaccggc tgatggcggt tgccaggtga cgctgatgac
      901 ggttgcatgt ccccgatatt cggcggggga tgatctttcc acgctccgtc agataatttc
      961 tgaggagcgt gatgtctttg tagtcaatgt gccgtacctt atccgtgcag aagcgacaga
     1021 gcttacggcg ctgaaaaaat ctcgttcgtt ccaaagtctc ctcctaaata agccaatcga
     1081 acattcttaa aacggcacat cgttgtccac cgcttctgtt tcaggttcag gcatcccctc
     1141 tccgcctaac gccgatttct ccgatggagc accctggcgt cggctcatga agcggacggt
     1201 ttgagccacg acttcatgct tgcttcgctt gacgccatcc tcggtctccc atcgccgctc
     1261 ttgaagccgg ccatccacga tcactccttg ccctttaccc agatactggc cacaattttc
     1321 ggcctgcttt ccaaacacca cgatatcaat atagcagact tcctctttaa actcttctcc
     1381 ctgcttgtac cggttgttca cggcaagcct gaaatttgcg acaggagtcc cgctcggagt
     1441 atatcggatc tcgggatcct tagtcagatt ccccatgagg atcaccttat tgaagctggt
     1501 cattgcaaac tctcctttcc gccgaaggcg acatgccctg cggagccccg cccgatccgc
     1561 ctcagacgga tggagcttcc tccagcgttg ctttctcgtg aacaggcttt ttctcagcgg
     1621 cctgtcccga tgcagaagtt gtcggcagtg gtccttctag tctaacggta ataaacttga
     1681 ggatggtttc gtccagtcgg tactgatgtt ccagctgtgc aatcagtttt ccatccgctt
     1741 gaaaacggaa gagcaggtag gctcccttct tctccctctt gatctcgtac gctagccttc
     1801 tctttcccca accctcggcc tggaggactg tgccgcccgc cttctcaatt atctccctga
     1861 tcttggccag gactgcattg atctcttctt ccgatagcgt ggatcgaaca attactatgg
     1921 attcataatt ctgcatctga tttcctcctc acgggttagt agccctaaca gcattgctag
     1981 agcgaggatc gcttttttgt tgtgtgtatg ataactcagc tctgcttcca gtgcaagcag
     2041 tcatttgaca ggctgcttat tatcggggct gaaaacggaa atagacaatg tctccatcct
     2101 gaacgatata gtctttacct tccaggcgaa gcagaccctt ctcccgcacc gaggccgatg
     2161 aacccgagga gataagatcc gaataacgga acacctcggc ccgaataaac ccttgttcca
     2221 tatcggaatg aatccttccc gcggcctccg gcgccttggc tccccttgga acggtccagg
     2281 cccggacctc ttcctcaccg gcggtgaaaa aggtgatcag acccaacaag gcataacctg
     2341 cccggatgag ccgatcgagg cctgattcct ttatccccag gtctgccaag aagactcccc
     2401 gctctccgat aggaagcgcc gcgatctcct gttccacctt tccgctgatg accacggcag
     2461 ccgcattttc ccgtttggcc gccttttcaa gctcctgaac atgccgattt tcacgattaa
     2521 gctcatcttc cgcgacattt gcgacataga gaatgggctt atcggtcaga aggcgatacc
     2581 catgcaaatg tgtctgctct tcaggggtgt aatctttccc gcgaatcggc tctccccgac
     2641 ccagcaggct ctggagccgc cgaaggaact ccatttcaac ggacgtctta ggatcgcctg
     2701 ccttgacccg cttctcaact ccctgaaggc gcttctctat cgtctcaagg tcggccagga
     2761 ccaattcggt atgaatcacg tcgatatcac ggaccggatc aacactgccg ctgacatgag
     2821 acacgtccgg gttctcgaaa catcgaacca catggacgat cgcgtccact tcccggattt
     2881 gcgcgaggaa cttgttgccg agcccttctc cctggctggc ccctttgaca aggccggcga
     2941 tgtccacgaa ctccaccgta gtgggagtga ccttcttggg ccggtagatc gccgttaagc
     3001 gttgaagccg ttcatccgga acctcgacga tccccacgtt gggatcaatc gtggtaaacg
     3061 gatagttggc cacctccgcc cgacccgcgg tcaaggcatt aaacagggtg gacttcccga
     3121 catttggcag accgacaatc ccgcactgaa aacccataaa cgtttcccgt caccaaaaat
     3181 atttcttgat caaacgtatg aaattcaatt atggaactga ttcatcgctt gagctaccgc
     3241 ttctgccacg acaagctcgg tcgccgctac ggcacggctg atcatttcct ccacgagagg
     3301 ccgctcctga gccgtgaagg ggctcaagac atgctcggct acctcttctc ctttgggacg
     3361 accgaccccc aatttgattc gtacaaaccg gtccgtctcc aagatttcca gaatggattt
     3421 tagaccgtta tgtcctccat agccgccgcg tgttttaatc cgcaaacaac cgaaatcgag
     3481 atcgatgtca tcatggatga caatcaggtc ggcaggactg atctggaagg ttttgcagag
     3541 gtcgcggaca gcggctcccg aacggttcat aaatgtcagc ggtttggcaa gcaccacctt
     3601 cgttgccttg atcgaaccct gcccatacat caccttctcg gttcgctctc ttaatgaaat
     3661 tcggtgttgc tgggccagcg catccacgac ccgataacca aggttgtgc
//
LOCUS       DATMKH010000345         2053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2397761, whole genome shotgun sequence.
ACCESSION   DATMKH010000345 DATMKH010000000
VERSION     DATMKH010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2397761"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VNV63_04200"
     CDS             <1..276
                     /locus_tag="VNV63_04200"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HXC61856.1"
                     /translation="AQYVVPFAYRIRWYMKLNLREAVHIGELRTMPQGHPDYRSIVQE
                     MWRKIQETHPTLAVCARFIDWKTYHLGRLQSEMRTEFKKATLEKGQA"
     gene            complement(273..959)
                     /locus_tag="VNV63_04205"
     CDS             complement(273..959)
                     /locus_tag="VNV63_04205"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HXC61857.1"
                     /translation="MLRAIIFDCDGVIVDSEPHHFKAFQQILSEEGIPFTKEEYFQKY
                     LALDDKTCFETALAAHGRAYDKKILKDLIIRKMEVYRQLSQQELFFYPGVVEFVKKAQ
                     GSYRLAIASGAFRGEIKFALDKAGLRPAFPVIVSAQDVRNSKPHPEAFLLALQQINER
                     PPALSPPILQKECLVIEDSIHGVEGAHAAGMRCLAVANSYPKEKLTHADLVIASLTEL
                     EPKDLEKLFV"
     gene            1125..>2053
                     /locus_tag="VNV63_04210"
     CDS             1125..>2053
                     /locus_tag="VNV63_04210"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HXC61858.1"
                     /translation="MTLPLPANKVDFGDQPFSPELMDSLFKMIRGKKKPFLGFLRAES
                     GAKQLFLFILESDPYAATLLEEETSRALSLRDFFIALNGMNRPVLSLYAANPVFIKCL
                     LVFSQKIPVTQGTTTLIDIEVLLRQLQTGKEDTVLALKKGKEVNLFYFLKGKLEELYF
                     ANPSGVPKEGSLEDQLLVYAFSGSKEMPIGVSVYKDLAVSPAEDADVSWAELSGGMTA
                     YFLRPRPELTVIKSDETIEKRLLQKRRYTIGRGETNDLTLDDAAASREHAVLEEKGGR
                     FYIEDRKSKNGTRLNGQRITTPTHVLSDGDEIR"
BASE COUNT          507 a          512 c          532 g          502 t
ORIGIN      
        1 gcccagtatg tcgtgccgtt tgcgtatcga atccgatggt atatgaaact gaatttacgg
       61 gaagcggttc acatcggcga gctgcgtacg atgcctcagg gacacccgga ctataggagt
      121 atcgtccagg agatgtggcg gaagatccaa gagacacatc cgacacttgc ggtctgtgcc
      181 cgatttattg actggaaaac ctatcacctc ggccgccttc aatctgagat gcgaacggag
      241 ttcaagaagg ccacgctgga aaagggtcag gcttagacga acaacttttc cagatctttc
      301 ggttccaatt ccgtaaggct tgcgatgaca aggtcggcgt gagttaattt ttcttttgga
      361 taggaattcg ctacggcaag gcatctcatc ccggccgcat gagccccttc gactccatga
      421 atcgaatcct ctatcacaag gcattccttc tgcaagatcg gtggggagag cgccggaggc
      481 ctctcattga tctgttggag ggccaggaga aatgcttcgg gatggggttt gctattccgc
      541 acatcctgcg cgctgacaat aaccggaaag gccggtcgca gtccagcctt atccagagcg
      601 aatttgatct cgccccggaa ggcgcccgat gcgatcgcca accggtagga gccctgcgcc
      661 ttctttacaa actcaaccac gccgggatag aaaaacagct cttgctggga gagctggcga
      721 tagacctcca tcttccggat gatcaggtct tttaagattt ttttatcgta cgctctacca
      781 tgagccgcaa gggcagtctc gaaacaggtc ttgtcatcca gcgcgaggta cttttggaaa
      841 tactcctcct tcgtgaaagg gatcccttct tcgcttagaa tctgctggaa ggccttgaag
      901 tgatgtggtt cgctgtccac aatcaccccg tcacagtcaa agattatcgc tcgaagcatt
      961 ttgttccgcg cctcataaag ggcctaatat accagatgca ggccaggttt tcaaaccggg
     1021 gttcacgttg acaaaatttc gattagaaat tataatgaat tcaggaccgt aacttatttt
     1081 tatatctaaa gaaaacggtt gtcactttaa taagggagcg acctttgacg ctgccgctgc
     1141 cggcaaacaa agtggacttt ggcgatcagc ccttctctcc agagctcatg gactctcttt
     1201 tcaaaatgat ccgtgggaag aaaaaacctt ttctggggtt tctccgggcg gagtcaggcg
     1261 caaaacagct cttcctcttt atactggaga gcgatcccta tgctgcgacc ctgttggaag
     1321 aagaaaccag ccgtgcctta agtctccgcg actttttcat tgcactgaac ggcatgaatc
     1381 gccccgtcct ttctctctat gcggcgaatc cggtcttcat caagtgttta ctggtctttt
     1441 ctcagaaaat accggtgacg caaggaacca ccacgttgat tgacattgaa gttctcctcc
     1501 gtcaactcca gaccggaaag gaggatacgg tgttggccct gaaaaagggt aaagaggtca
     1561 atctctttta cttcttaaaa ggaaagcttg aagagctcta ctttgcgaat ccgtccggtg
     1621 tccccaagga gggatccctg gaggatcaac tcttggtgta tgccttttca ggttccaagg
     1681 aaatgcctat tggtgtgtcg gtttacaaag acctcgccgt atcacctgcg gaagatgcgg
     1741 atgtctcatg ggcggagctt tcaggcggga tgacggctta tttccttcgg ccgcgtcctg
     1801 aactgacagt tattaaaagc gatgagacga tcgagaagcg gctgctccaa aaacggcgat
     1861 acaccatcgg gcggggagag acgaatgatc tgacacttga cgatgcggct gcatcccgtg
     1921 aacatgccgt cttagaagaa aaaggcggca ggttttacat tgaagatcgg aagagcaaga
     1981 acggtacacg gctgaatggt cagagaatta cgaccccgac ccatgtactt tcggatggcg
     2041 atgagatccg gat
//
LOCUS       DATMKH010000346         1066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2084447, whole genome shotgun sequence.
ACCESSION   DATMKH010000346 DATMKH010000000
VERSION     DATMKH010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2084447"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..916)
                     /locus_tag="VNV63_04215"
     CDS             complement(<1..916)
                     /locus_tag="VNV63_04215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HXC61859.1"
                     /translation="MSGTDQHFTFLNQGWLIFTGRSMEQELGEGWVSGVHPDDLENCL
                     RIYSAAFDARAGFEREYRLSRFDGEYRWILDFGTPRFESDGTFCGYIGSCVDISERKL
                     SEASLQNLSGRLIRAQEEERSRIARELHDDFSQRMALLSIGLVQLWKRLPPSEVEERA
                     RVWEMQQRSQDMTDDMHSLSHKLHSSKLELVGLVSALHGLCQELSEKYKIDIQFSACE
                     LPFNVPKDVALCLFRVTQESLGNVIKHSQANSACVELDANGSGVSLRITDAGRGFDPD
                     VSNPYAGIGLAGMRERLRLIGGRLSINSE"
BASE COUNT          254 a          309 c          260 g          243 t
ORIGIN      
        1 gttccgaatt gatagagagc ctccccccga taagccgaag acgctcgcgc atacctgcca
       61 atccgattcc tgcgtacggg tttgaaacgt cagggtcaaa ccctctgccc gcatctgtaa
      121 tgcgaagact cactccgctt ccgtttgcat ccagttccac gcatgcacta ttggcctgac
      181 tatgtttaat gacattgccg agcgattcct gggtgactcg aaataaacaa agagccacat
      241 ctttcgggac attgaaaggc aattcgcagg cactaaactg aatgtcaatc ttgtatttct
      301 cactgagttc ctgacaaaga ccatggagag ccgacaccag acctaccagc tcaagcttgc
      361 tggaatggag cttatgagag agagaatgca tgtcatcggt catatcttgg ctacgttgct
      421 gcatttccca gaccctggct cgttcctcca cttctgatgg aggtagtctc ttccatagtt
      481 gtacgaggcc aatactcaag agcgccatac gctggctaaa gtcatcatgc aactccctgg
      541 cgatgcggga ccgttcttcc tcctgggcgc ggatcaggcg cccgctcaaa ttctggaggg
      601 atgcttcgga caactttcgc tccgaaatgt caacgcacga tccaatatag ccgcagaagg
      661 tgccatcgga ttcaaaccgg ggcgtgccaa aatcaagaat ccagcggtac tctccatcga
      721 agcgggacag gcggtattcc ctttcgaacc cggcccgagc gtcaaaggcg gccgaataga
      781 ttcgcagaca attctcaagg tcgtctggat gtactcccga aacccatcct tctcccagct
      841 cctgttccat ggatcggccc gtgaagatca accaaccttg attcaaaaaa gtgaaatgct
      901 ggtccgtgcc ggacatccag atcagcacag gagcggtgtc ggcgacccgg cggaaacggt
      961 tttcactctc ccgaagaaca gcttctgacc tcttgagttc cttattgctg gcgacgaggg
     1021 actgctcgac agctctcctg ttcgctcgct gctttagcag gccaag
//
LOCUS       DATMKH010000347         1748 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_164046,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000347 DATMKH010000000
VERSION     DATMKH010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164046"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..878
                     /locus_tag="VNV63_04220"
     CDS             <1..878
                     /locus_tag="VNV63_04220"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HXC61860.1"
                     /translation="LVFQGTPDAKDLRGEITIKRALYGKRANIQAAAVSALEAKPEVP
                     KSGEVPIFGDTALNIHLSGKDHIWLNNNVAKLPLQVDLVLRGTLSRPILLGRIEAQEG
                     SVFFRKNEFKVTTAVVDFIDPERPKPVFEIEAKTEIRKNRITIRLSGTPDKFRVNLTS
                     DSPLNETDLVALLLVGKTAKEVTQLTPGLGVAEAPLLIPEELQRRLLATTGRFFMPPS
                     SDPGGSRKTPRFAIAKKLIGDKLFLIYAVNLETSEEHLIRIEQNIGDNRVLIGERDPE
                     NGMSHEIVVRIESKA"
     gene            875..>1748
                     /locus_tag="VNV63_04225"
     CDS             875..>1748
                     /locus_tag="VNV63_04225"
                     /inference="COORDINATES: protein motif:HMM:NF018899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HXC61861.1"
                     /translation="MSSYHLTEKTGFLKWPLRIIFWAVLVWLSLLQPARPADLPVEQA
                     GSEGKPIRSIAVISPRGFSSQDVQQITGIKIGEPYSTTRIRQGLERLYRTGDFRDILI
                     ETEPKADGIQVTFTLVDKPPISEIRLKGNGTFPSNDLQRVMQVKTGDEFSNAGWKAAL
                     ARLVTYYRQQGFLRARIVSETSTIEKENRVILVAEIQEGQRARVRDIQFHGDSVFPPA
                     KLKTIIKTTPGGPYQTDQMEDDLKALEQFYTKEGYLKATIGPPNITYHENTNEVVIRF
                     PIDAGIQLKVTFTGN"
BASE COUNT          451 a          488 c          458 g          351 t
ORIGIN      
        1 cgctggtttt ccagggaacg cccgacgcga aagaccttcg cggtgagatc acgatcaaac
       61 gggccctcta tgggaagcgc gccaatatcc aggccgccgc ggtctccgcg ctggaggcga
      121 aaccggaggt tccgaaatcc ggagaagttc cgatcttcgg agacaccgcc ctcaacatcc
      181 acctctcggg caaagaccac atctggctaa acaataacgt cgctaaactg ccattgcagg
      241 tagacctggt tctgagaggc acgctgagcc gcccaatcct tttggggagg atcgaggcgc
      301 aggagggatc agtttttttc cggaagaatg aatttaaggt cacaacagcg gtggtggact
      361 tcatcgatcc ggaacggccc aagcctgttt ttgagatcga ggccaagacg gagatccgca
      421 aaaatcgtat cacgattcgc ctgtccggca cgccggataa attcagagta aaccttacct
      481 ctgactcccc gctcaatgag accgacctcg ttgctctgct tctggttggg aaaaccgcta
      541 aggaggttac ccagttgacc ccgggtctcg gcgttgcgga ggcaccgctc ctgatcccgg
      601 aagagctgca gcgtcgtctt ctcgcaacaa caggccgttt tttcatgccg ccctcttctg
      661 atcctggcgg cagtcgcaaa accccgcgct ttgcgattgc aaagaaactc atcggagata
      721 agctctttct gatctatgcg gtgaatctcg aaacctccga agagcatctc atccggatcg
      781 aacaaaacat aggagataat cgtgtattaa tcggtgaacg ggatccggaa aacggcatga
      841 gccatgagat tgtggtgagg atagaatcaa aggcttgagc agctaccatc taacagagaa
      901 aaccggtttt ttaaaatggc ctctgagaat cattttttgg gcggttctcg tgtggctttc
      961 cctcctccag ccggcccgac ctgcagatct gcctgtagaa caggcaggca gcgaaggcaa
     1021 acccattcga tccattgctg tgatctctcc gcgtggattc agcagccagg acgtccagca
     1081 gatcacggga ataaaaatcg gcgaacccta ttccaccacg cgtatccgcc aaggtcttga
     1141 acgactgtat cggaccgggg atttccggga catcctgatc gagaccgaac ccaaggcgga
     1201 tgggatccag gtcaccttca cgctcgtaga caaaccgccc atctccgaga tccggctgaa
     1261 agggaacggg acatttccat caaatgatct tcaacgggta atgcaggtaa aaaccggtga
     1321 tgagttcagc aacgcgggat ggaaggctgc cctggcacgg ctcgtcacct attatcgcca
     1381 acaaggattt ttgagagccc ggatcgtctc tgagacctcc acgattgaga aagagaaccg
     1441 ggtaatcctg gttgcggaaa tacaggaagg ccaaagggct cgggttcgcg acatccagtt
     1501 ccacggcgac tctgtgttcc caccggcaaa gctcaagacc atcatcaaaa caactccggg
     1561 cggaccctac cagacagacc agatggaaga cgatctcaaa gctctggagc agttctatac
     1621 aaaggaaggc tatctcaaag caaccatcgg tccacccaac atcacgtacc atgaaaacac
     1681 caacgaggtg gtgatccgtt ttccgataga cgccggcatc caacttaagg tgacctttac
     1741 gggaaacg
//
LOCUS       DATMKH010000348         1278 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1126764, whole genome shotgun sequence.
ACCESSION   DATMKH010000348 DATMKH010000000
VERSION     DATMKH010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1126764"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="VNV63_04230"
     CDS             <1..792
                     /locus_tag="VNV63_04230"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXC61862.1"
                     /translation="LVATATLAATLNIPTSDPRPATMGRILIIEEDGALRKTLRRLFS
                     SEGYEVDVVPDRMAGLEMLRQRSPSAMILDLQDAEPSGCNLCRKIADLVPGLPLVILS
                     ASSKVADKVLLLEMGADDYLTIPFSPRELVARLRALIRRASRSSPEDAYAYVFADVTV
                     DFFRTEITRCGEKIMFTQMEFKMLEFLTKNAERVISRGELLKEVWGYQHCPCTRTVDN
                     HILKLRQKLESDPQHPLHFLTVHGRGYKFLPKTEQRKEPGHRGVH"
     gene            789..1092
                     /locus_tag="VNV63_04235"
                     /pseudo
     CDS             789..1092
                     /locus_tag="VNV63_04235"
                     /inference="COORDINATES: protein motif:HMM:NF017534.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          302 a          318 c          350 g          308 t
ORIGIN      
        1 cttgtggcaa ccgccacctt agcggcaacc ttgaacattc caacctccga cccacgaccg
       61 gccaccatgg gtcgaattct gatcatcgag gaggatggtg ctttgcggaa gactctgcgg
      121 cgacttttct cctcggaagg atatgaggtc gatgttgttc ccgatcgtat ggctggtctg
      181 gagatgcttc gccaacgatc accttccgca atgatcctcg atctacagga cgcagaacct
      241 tcggggtgta atctctgcag gaaaattgcg gatctggtcc ccggtctgcc cctggtgatt
      301 cttagcgcga gctcaaaggt tgcggacaag gtccttctcc tcgaaatggg tgccgacgat
      361 tacctgacaa taccgttcag cccacgggaa cttgttgcgc gtttgcgcgc gttaatcagg
      421 cgtgcgtcgc gctccagtcc ggaggacgcc tacgcatatg tctttgcgga tgtgacggtg
      481 gattttttca ggacggagat cacacgatgc ggcgagaaga tcatgtttac tcaaatggaa
      541 ttcaagatgc tggaattctt gaccaaaaac gctgaacgtg tgatctcacg gggcgagctt
      601 ctcaaggagg tttggggtta ccagcattgt ccttgtaccc gaacggtgga caaccacatt
      661 ttgaagttac gacagaaact ggagagcgac cctcaacacc cgttacactt tctcacagta
      721 cacggccggg gctataagtt tttgcctaaa acggagcagc gcaaagagcc ggggcataga
      781 ggtgtccatt gagcttaaga gagaaaagag ttgctgctat cggttctcag ttttggatag
      841 cggcctggaa tgaaggaaga acaaatttcc accggggaaa ttatcacgat aaacttacgg
      901 agtatttccc tcagttaaat cctggagagg ggcagagagt actcgtccct atgccggaaa
      961 tcgaccgatc ttctttggct ccatgaattg aacctctgtg ttcatgggac agaactgcac
     1021 gatcaggcgg tggaatcttt tttcgcggaa aaagaactct atgtcgttga gaaggaacga
     1081 gcgcttggat aggccaaatt cactgttcct tgaaccagag cagttcgttg ctcagtgtaa
     1141 tgagtggttc tccggatggc gtcagttcga tggaatgtcc gccacgctct tctaggatat
     1201 cgagatgacc caatatcttg ccgctccgcg gatcgagctt gaccacccag cccggcgcac
     1261 ctggtggttg atcgaaag
//
LOCUS       DATMKH010000349         1654 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_166586,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000349 DATMKH010000000
VERSION     DATMKH010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166586"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="VNV63_04240"
     CDS             complement(<1..400)
                     /locus_tag="VNV63_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HXC61863.1"
                     /translation="MMSSDLSLQKLPPQNLEAEQSVLGAILLDNPALNKALEILAPDD
                     FYKDAHRRIFSAVLDLNERNEVIDLITITEHLRQKNELEQVGGAAYLAALVNSVPTAA
                     NIRFHSKIIHEKALLRNLIQIATEIVTMGYE"
     gene            complement(397..>1654)
                     /gene="hisZ"
                     /locus_tag="VNV63_04245"
     CDS             complement(397..>1654)
                     /gene="hisZ"
                     /locus_tag="VNV63_04245"
                     /inference="COORDINATES: protein motif:HMM:NF024785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HXC61864.1"
                     /translation="ILPRGIAAFRPVAVAQKRGIERELLSIFSQWGYEEVIPPIFEYL
                     DVLADGLDEEMITKGYKVVDRGTGRLLILRPDVTPQIARMAATLMRDRPLPLRLCYSA
                     NVFRDEEEHAGRERELFQIGCELIGLEAPQADAEIIAIAIETLRHLGLRHFKLAIGQV
                     EFFRGFLEGLRLSPQIQKKIRNAMARKESSELREIIQQARIPSKVAEKILAVPELFGK
                     AEVLKQAGHLVNGSATERALARLEEVYQLLVDYGYREHLIIDLGEIRGFDYYTGIVFE
                     VFAKGLGYAFGGGGRYDQMLGKFGYDCPSTGFAFDLERLYNVLKQAEGAANDHPLNYL
                     ITNSSKQAVRAFQLAKALREKGCRVTLRTWNGDFSDTIRYARGIGVEKVLLLEDQERA
                     SDEVVLLDCRNGSRKRFKGRKVLEIL"
BASE COUNT          405 a          466 c          382 g          401 t
ORIGIN      
        1 cctcgtagcc catcgtaacg atctcggtag cgatctggat cagattccgc aagagggctt
       61 tctcatgaat gattttggaa tggaatcgaa tattggccgc ggtgggaacg ctgttgacca
      121 aggctgcaag ataggcggct cctccgactt gctccaattc attcttttga cgtagatgct
      181 ccgtaatcgt gatcagatca atcacctcgt tacgctcgtt gagatctaag accgccgaaa
      241 aaattctccg atgggcatct ttatagaaat catcgggcgc gaggatctct aatgccttat
      301 tgagcgctgg attgtccaag aggatcgcac ccaggacaga ctgctccgct tcaagatttt
      361 gaggtggaag cttctgaaga gaaagatcgg aagacatcat aagatctcca aaaccttcct
      421 gcccttaaat cgtttccgcg aaccgtttcg gcaatctaac agcacgactt cgtccgaagc
      481 tcgttcttgg tcttccagga gcaggacctt ttctactccg atccctcggg cgtaacggat
      541 ggtatccgaa aaatccccat tccaggttcg caacgtgacc cggcaccctt tttcccgaag
      601 tgcttttgcc aattgaaatg ccctgaccgc ctgcttcgaa gaatttgtga tcaaataatt
      661 caatggatga tcgtttgctg ctccttcggc ctgcttgagg acattgtaga ggcgctcgag
      721 atcaaaggca aatccggtgg acggacagtc atacccgaat ttccccagca tttgatcgta
      781 ccgcccgccc cctccaaaag catagcccaa tccttttgca aaaacctcaa acacaatccc
      841 tgtataataa tcaaatcccc ggatctcgcc cagatcaata atcagatgtt cacgataacc
      901 gtaatcgacc agtagctgat agacctcctc tagacgggcc agcgctcgtt cggtcgcgct
      961 gccattcacc agatgacctg cctgcttaag aacctccgcc tttccaaaaa gctccggaac
     1021 cgctaaaatt ttttcggcaa ccttagaggg aatccttgcc tgttggataa tttctcgaag
     1081 ctccgagctc tctttgcggg ccatcgcatt tctaattttt ttctgtatct ggggcgaaag
     1141 tctgagtcct tccagaaaac ccctaaagaa ctctacctgt cctatcgcca acttgaaatg
     1201 cctcagaccc agatgccgta gagtttcaat cgcgatggcg atgatctcgg catcagcttg
     1261 aggggcctcc agaccgatca gctcgcagcc gatttgaaat agctcccgtt ctcggcccgc
     1321 atgttcctcc tcatctcgaa agacattggc gctgtagcac agcctcagag gaagcggacg
     1381 atcacgcatc aaggtcgccg ccatgcgtgc aatctgcggg gtcacatcgg gccgcagaat
     1441 aagaagccgg ccggttccac ggtccacgac tttgtagcct ttcgtaatca tttcctcatc
     1501 caagccgtcg gccaggacat cgaggtattc aaaaatggga ggaatcactt cctcataacc
     1561 ccattgagaa aagatggaga gaagctcccg ttcaataccg cgtttctgcg cgaccgcgac
     1621 ggggcggaaa gctgctattc ctctgggaag gatg
//
LOCUS       DATMKH010000350         1318 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3371818, whole genome shotgun sequence.
ACCESSION   DATMKH010000350 DATMKH010000000
VERSION     DATMKH010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1318
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3371818"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VNV63_04250"
     CDS             <1..318
                     /locus_tag="VNV63_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXC61865.1"
                     /translation="FSRVVVGTAKDAIHSLRKQKFDLMFLDLQLPDAPGDEVYKTAKS
                     LDPNLNVIVITGYPDSQMLDRILQISPVTVLKKPLKIEQLNQTVKILGHTSSKIEPYA
                     MHR"
     gene            601..987
                     /locus_tag="VNV63_04255"
     CDS             601..987
                     /locus_tag="VNV63_04255"
                     /inference="COORDINATES: protein motif:HMM:NF016557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61866.1"
                     /translation="MIGGFTEPEGSRKYFGALLVGFYEGKKLKFAGRVGTGFSDKLLS
                     TLYSELDKIRIEKCPFYNLPVAGRNRWDQGLTAAEMKRCQWIKPEMVCQIKFTEWTRD
                     DRLRQPVFLGIREDKRPEEVLREKAN"
BASE COUNT          351 a          323 c          361 g          283 t
ORIGIN      
        1 tttagtcgag tcgtggtcgg aacagccaag gacgctatcc acagtctaag aaaacaaaag
       61 ttcgatctga tgtttttgga ccttcaatta ccggacgcac ccggagatga agtgtacaag
      121 accgcgaaga gcctcgatcc caatctgaat gtcatcgtga ttaccggcta cccggacagc
      181 cagatgctgg acaggatttt acagatcagt cccgtgaccg ttctgaaaaa gccactcaag
      241 atcgagcagt taaaccagac ggttaaaatt ctcggccata cctcttcaaa aattgaaccg
      301 tatgcaatgc accggtgatc attttgctgc ggcagtcagc acctccaccc tgaactgcgt
      361 ttgatcccgt gggagaaggg tgtccctcac cgggacgcac gaaaggacga ggaaaggtga
      421 cgtatcgcca ttcatattgg accagaacat cgtgcttagc gcggcatctc gtgtttcctt
      481 ttcgagagaa agccgtacag gccggacagc ggtccgcgga aaaagccgag ttcccggtat
      541 gagcccggaa agcgcacagg agcgtggatc aaggttaagc tccaccaaga gcaggaattc
      601 gtgatcggcg ggttcacgga accggaagga tcgcggaaat attttggcgc actgttggtg
      661 ggtttctatg agggcaagaa acttaaattt gctggcagag tcggaactgg ctttagcgac
      721 aaacttctga gcacgctgta ttcggaacta gacaaaatca ggattgaaaa atgtccattt
      781 tataaccttc cggttgctgg ccggaaccgt tgggatcaag gtctgaccgc tgccgaaatg
      841 aagcgttgcc agtggatcaa accagaaatg gtgtgtcaga taaaattcac agagtggacg
      901 cgggatgacc gtctccgaca accggttttt ctaggaatca gggaagataa gaggccggag
      961 gaggtgctgc gggagaaggc aaactaagaa acttcggttt aagctttagt cccaaagaat
     1021 ggcaagcctg ccgctaacag aatcccccct gcgtcgccgc caccgatttc ccgactccag
     1081 atgagccgat catcgtggca aaagttccac gctctagcca acggtcaccg aggagatttt
     1141 gggactcatc gattttggca tttaataaat cgccaaagac gctttcaaag gtaaggctca
     1201 tactgactcc gtttcggttg cgtgaacact atcatgggct ttcgcggagg agcgtcgtta
     1261 tcgctcgaaa gccggggaag ttgccggtgg cgagagaatc gcggcccgct tcacgaac
//
LOCUS       DATMKH010000351         1043 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1129619, whole genome shotgun sequence.
ACCESSION   DATMKH010000351 DATMKH010000000
VERSION     DATMKH010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1129619"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="VNV63_04260"
     CDS             complement(<1..285)
                     /locus_tag="VNV63_04260"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HXC61867.1"
                     /translation="MTSALAQPAVSPLGTIRLASFVTNGSGSGKGKLRRYPSYALVNL
                     LDGLGYYRDSTGFTQPVHPGDLIWVSPRVAHTYGPQEGHFWNEFFVVFDGP"
     gene            383..>1043
                     /locus_tag="VNV63_04265"
     CDS             383..>1043
                     /locus_tag="VNV63_04265"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HXC61868.1"
                     /translation="MAQICSGPPRSGGKILDNQDDVFGSLSDSSDSLANPAELRSLLE
                     SDGYLYLKRFFNPTDVLEIRKSILARMLEEDLVLPGTNPVDAKANPDRRTAFMPELAE
                     NNLALEKLIYGERVMTFYETLLEGPVLHYDFTWFRPVGPGRGTSPHCDLVYMGRGTPQ
                     VFTMWVPYGEVSLSLGGLMVLEGSHRKSKLLGNYLSRDVDSYCANRQGAEKAQTSDSF
                     LW"
BASE COUNT          246 a          277 c          282 g          238 t
ORIGIN      
        1 cggaccgtcg aaaacgacaa aaaactcgtt ccagaaatgt ccttcctgtg gaccgtaagt
       61 gtgcgccaca cgaggagaaa cccaaattag atctccggga tggaccggtt gggtaaaacc
      121 cgtgctgtct cgataatatc ctagcccatc gagaagattc acgagcgcgt aactcggata
      181 gcggcggagc ttgcctttcc cgctcccaga gccattcgtc acgaagctgg ccagacgaat
      241 tgttccgaga ggcgacactg ccggttgcgc cagcgcagag gtcatcacat tccgacggtc
      301 cacaagccga acaataccat gttacacatg ggatttccca ccagatccat tcataatcga
      361 ctgccaggac gtaggatgac ggatggctca aatctgctcc ggtccgccac gatctggcgg
      421 gaagattttg gataaccagg acgacgtttt cgggtcgtta agcgattcgt ctgactcact
      481 agctaaccct gccgagctac gcagcctcct agagtcggat ggctatcttt acctcaaacg
      541 cttcttcaat ccgacagacg tcttggaaat cagaaagagc attctggccc ggatgctcga
      601 agaagatttg gttctccccg gcaccaaccc tgttgatgcc aaggctaacc ccgatcggcg
      661 aaccgctttt atgccggagt tggcggaaaa caatcttgcc ctggagaaac tgatttatgg
      721 cgagagagtg atgacattct acgagaccct tctggagggg ccagtgctgc actacgattt
      781 tacctggttc cggccggtcg gcccgggccg tggaacctca ccgcattgtg atttggttta
      841 tatggggcgc ggaactccac aggtctttac aatgtgggta ccttatggcg aggtctctct
      901 gagccttggg ggtctgatgg tgctggaagg gtcacaccgc aagagtaagt tgttgggaaa
      961 ttacctctct agagatgtcg attcatattg cgctaatcgt caaggggccg agaaagcgca
     1021 aacctcagat tcgtttcttt ggg
//
LOCUS       DATMKH010000352         1155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1763121, whole genome shotgun sequence.
ACCESSION   DATMKH010000352 DATMKH010000000
VERSION     DATMKH010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1763121"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..>1155)
                     /locus_tag="VNV63_04270"
     CDS             complement(57..>1155)
                     /locus_tag="VNV63_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXC61869.1"
                     /translation="HAQSVMDGKIPRCKLTAVCDPKVERLDPFPSAEGFLSVEDFLRS
                     SGTDAVLIATPHYSHTDIGIRALESGRHVLVEKPISVHKSDAERLVAAHHSSEQVFAA
                     MFNQRTDPFYLKLRQLVQSGDLGAIRRVNWTITDWFRTEAYYQSSDWRATWAGEGGGV
                     LLNQCPHNLDLIQWIFGMPERVRAFCHFGRYHDIEVEDDVTAYLEYSDGMTAIFSAST
                     GEAPGTNRLEVAAEQGKVVIENDSFLFTRNEVPMSEFSRTDPGRFSAPATWEIRIPIT
                     SHGPQHNGVLVNFVSAILERTPLVAPAREGLNSLELANAFLLSTLENRSVELPIDGAL
                     FEEHLLKLIASSGQKAKVSKAVSDDMANSFR"
BASE COUNT          282 a          310 c          304 g          259 t
ORIGIN      
        1 tcggagcgta atcgtaacct gactgactca tgatgcaagg acctgttcgt ggttagtcat
       61 cgaaaagaat tcgccatatc gtctgagacc gccttggaaa cttttgcttt ctgtccggat
      121 gaagcgatca attttaggag atgctcttcg aacagggctc catcgatagg aagctcgacc
      181 gatcggttct ctaaagttga aagcagaaac gcgtttgcta attccaaaga gttaagacct
      241 tcccgagccg gcgcaaccaa cggcgttctc tccaggatgg cagagacaaa attcaccagg
      301 actccattgt gctgggggcc atggctagtg atggggattc ggatttccca agtcgctggc
      361 gccgaaaacc gaccaggatc cgtccgtgaa aactcactca tcggcacctc atttcgagta
      421 aagagaaatg aatcattttc gattactact ttgccctgtt cggcggccac ttcgagccgg
      481 ttagtaccgg gtgcttcccc ggtggaagcg ctgaagatgg ccgtcatgcc atccgagtac
      541 tccagataag cggtcacatc atcttcgact tcgatatcgt gataccggcc aaaatggcaa
      601 aaggcgcgga ctctctcagg cattccaaag atccattgaa tcagatccag attgtgggga
      661 cactggttga gcaacacacc tccaccctca cctgcccagg tagctctcca atcgctcgac
      721 tgataatagg cttcggttcg gaaccagtca gtaatcgtcc aattgacgcg cctgatcgct
      781 ccgagatcgc cggactgcac caactgacgg agcttcagat aaaaagggtc cgtccgctga
      841 ttgaacatcg cggcaaagac ctgttccgag ctgtgatggg ccgcaactaa gcgttcggca
      901 tccgatttgt ggactgagat cggcttttca acaagcacat gtcggccact ttccagtgcc
      961 cggataccga tgtcggtgtg ggaataatgc ggcgtcgcga tcaggacggc gtctgtcccg
     1021 gaggagcgca ggaaatcctc cacagaaaga aaaccttcgg ccgacgggaa aggatcgagg
     1081 cgttcgacct tcggatcaca taccgcggta agtttgcagc gtggtatttt accatccata
     1141 acgctctggg catgg
//
LOCUS       DATMKH010000353         1258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4642881, whole genome shotgun sequence.
ACCESSION   DATMKH010000353 DATMKH010000000
VERSION     DATMKH010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4642881"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1258)
                     /locus_tag="VNV63_04275"
     CDS             complement(<1..>1258)
                     /locus_tag="VNV63_04275"
                     /inference="COORDINATES: protein motif:HMM:NF019135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HXC61870.1"
                     /translation="RFWVADGENLEEFDRASGKVLSQVRLSEGIKDVSFYEDHRGVFW
                     VTYITSSSGSGLAVLDRSANRLVPFSIYDKKSGKEIPAGFMAAVEDKSNTLWLASFGG
                     GLLKFDGDRGLLVRYRSNPGNPESLADDRVISLCEDREGHIWVGLDASEPNFFRIERT
                     PFVPLPPSRSSPGSHGGKFVNAVYEDHRGVLWTATRGALNRINRGSGESISYRSPGTE
                     SNDVITITEDQRGDLWVGTFGAGLSRFDRKTGRFKTYLHFPGIASSLSSNIVSRILID
                     HAGTMWIATYNGLDRFDPRNESFVVYKQDPKSDDEEYFNLAEDKSGFLWMGSWGGLNR
                     FDPETGQFTVYAHRVSDPSSLSDNAVTSTYVDHSGALWVSTENGLNKLNRESGMFTHY
                     YVKDGLPSSAVSCVLEDQFSRLWMST"
BASE COUNT          302 a          363 c          293 g          300 t
ORIGIN      
        1 ttcgtgctca tccagagcct gctgaactga tcctcaagaa cacagctcac tgcactgctt
       61 ggtaaaccat ctttgacata gtagtgagtg aacattccgc tttcgcggtt cagcttgttc
      121 aagccatttt ccgtgctgac ccagagggcc cctgaatggt ctacataagt cgaggtgacg
      181 gcgttgtcac tcaaagaact gggatcactc actctgtgtg catagacagt gaattgaccg
      241 gtctccgggt cgaaacggtt caaacctccc caacttccca tccacaggaa cccgctcttg
      301 tcctcagcga gattgaaata ctcctcgtca tcgctcttgg ggtcctgttt gtaaacaacg
      361 aagctctcat tccgagggtc aaatcgatca agaccgttgt aggtggcgat ccacatcgtg
      421 cctgcatgat cgatcagtat ccggctcacg atgttgctgc tcagactgga tgcgatgcct
      481 ggaaagtgca gataggtctt gaaacgcccc gttttccgat cgaaccggct tagtcccgca
      541 ccaaatgttc cgacccagag atctccacgc tgatcttcag tgatcgtgat cacatcattg
      601 gattccgttc cgggagaccg gtaagaaatg gactctcctg agcctcgatt gatacgattt
      661 agcgctcctc tcgtcgcagt ccacagtacg ccccggtgat cttcgtagac agcgttgacg
      721 aactttccac cgtgactgcc gggactactt cggctaggag ggaggggcac gaatggcgtc
      781 ctctcgatac gaaaaaagtt tggttcgctt gcatccaaac ccacccaaat gtgtccttcg
      841 cggtcctcgc aaagcgaaat cacgcgatca tcggcgaggc tctcaggatt tccagggtta
      901 ctccgatagc ggacgaggag gccacgatcg ccatcgaact tcaacaaccc cccgccgaaa
      961 cttgctaacc aaagagtgtt gctcttgtct tcaacggcgg ccatgaatcc agccggtatt
     1021 tctttcccag atttcttgtc ataaattgaa aaagggacaa ggcgattcgc ggatcgatca
     1081 agaacagcta atccgcttcc actggaactt gtgatatacg taacccaaaa aacgccgcga
     1141 tgatcctcat agaacgaaac gtctttgatc ccttcactta ggcggacttg tgacaggact
     1201 tttccactcg cacgatcaaa ctcctcaagg ttctccccat cggctaccca gaaccgac
//
LOCUS       DATMKH010000354         1253 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2402428, whole genome shotgun sequence.
ACCESSION   DATMKH010000354 DATMKH010000000
VERSION     DATMKH010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2402428"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1253)
                     /locus_tag="VNV63_04280"
     CDS             complement(<1..>1253)
                     /locus_tag="VNV63_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002616629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HXC61871.1"
                     /translation="TQKNQEQDWLKTNLARFSGMMQGQRSIVAVAQLIMSELTPLVDA
                     QHGTFYMLQQDEQEPALHLIASYAFMNRKGISNRFKLQEGLIGQCAFEKKRILLTKVP
                     DDYIAVSSSLGESLPLNILVLPVLFEGELKAVIELASIHSFSENYLNFLDQLMDSVGV
                     ILNMISSTMRTEELLQEVKRSNAELEAQAKELEDKAKLLEVKNQEVELASLSLQEKAE
                     QLSLISKYKTEFLANMSHELRTPLNSLLILSKLLSENKDGNLTAEQVKFAGTVYASGC
                     DLLALINEILDLSKVEAGKMPIEPKIVQIEELRRSLEQTFRPVAEHKGLNFIIEIDPD
                     LPGNLYTDNFRLHQILKNLLSNAMKFTEQGSVTLQVRPEVQRRQFGVDALNRAKYVLA
                     FIVRDTGIGIPQDKQKLIFEAFQQA"
BASE COUNT          289 a          318 c          321 g          325 t
ORIGIN      
        1 ctgcctgctg gaacgcttcg aatatgagct tctgtttgtc ctgcgggatg ccgatccccg
       61 tgtcacgcac gataaaagcc aagacgtatt tcgctctgtt gagggcgtct acgccgaatt
      121 ggcggcgctg gacctcgggt cggacctgca gggtcacgct tccctgttcc gtaaacttca
      181 tggcattgga aagcaggttt ttgagaatct gatgcagacg gaagttatcc gtataaagat
      241 tgccgggtaa atctggatcg atctcaatga tgaagtttaa acccttgtgt tccgccacgg
      301 gccggaacgt ctgttccaag gaacgacgaa gctcttcaat ctgaacaatc ttgggttcaa
      361 tcggcatctt gccggcctcg actttggaga ggtccagaat ctcattaatc aaggccaaca
      421 gatcgcaacc ggaggcgtac accgtacccg cgaacttgac ttgttctgct gtgagattgc
      481 cgtccttgtt ttcagaaagc agtttggaaa gaatgagcag gctgttcagc ggggtccgga
      541 gttcatggga catattcgcc aggaactctg ttttgtactt tgagataagg gacagctgtt
      601 ccgccttttc ctgcagggaa agactcgcca gctccacttc ctggttcttc acttcgagca
      661 atttggcctt atcttcgagt tctttggctt gcgcttcgag ttcagcattg gaccgtttga
      721 cttcctgaag gagctcttcg gttctcattg tcgaagagat catattcagg atgacaccga
      781 cagaatccat caactgatcc agaaagttga ggtagttctc agaaaacgaa tggatcgaag
      841 ccagctcaat aacggctttc agctctcctt cgaacaagac tggcaaaacc agaatgttga
      901 gcggcaggga ctccccgagg cttgaggaga cagcgatata atcatccggg accttggtca
      961 gaagaatccg cttcttttca aaagcgcatt gaccgatcag cccctcttgg agtttaaagc
     1021 ggttggagat ccctttccga ttcataaagg cataactggc aatcaaatgc agggccggct
     1081 cttgttcatc ctgctgcagc atatagaacg tcccgtgctg ggcatccacg agaggagtca
     1141 gctccgacat aattaactgg gccaccgcca cgatgctccg ttgcccctgc atcatgccgg
     1201 agaaacgtgc caggttcgtt tttaaccagt cttgctcctg gtttttttgc gtg
//
LOCUS       DATMKH010000355         2600 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1449027, whole genome shotgun sequence.
ACCESSION   DATMKH010000355 DATMKH010000000
VERSION     DATMKH010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1449027"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="VNV63_04285"
     CDS             <1..411
                     /locus_tag="VNV63_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXC61872.1"
                     /translation="DEANPLESGLDIIAQPPGKRFRNISLLSGGERALTAIALLFSSF
                     LIHPSPFCVLDEIDAPLDEENNRRFLTVLKRLTDRSQFIIITHSKRTMEAASVLYGVT
                     MGEPGVSKLVSVHLEKHSPTETDSSVKDQAVLQL"
     gene            complement(427..900)
                     /locus_tag="VNV63_04290"
     CDS             complement(427..900)
                     /locus_tag="VNV63_04290"
                     /inference="COORDINATES: protein motif:HMM:NF021363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HXC61873.1"
                     /translation="MIKKVKEQFRKLLHLNDTPHRIAVAFALGIYIAFSPLIGLHTGM
                     VFLFAWLFRLNTIALLLGSLLNNPWTFGPIFGSCLWLGLKLYGMEFNGMKRTVPPIDW
                     PHLTMLNFMSQLKPYLIPFVLGTTLVGVIASAIAYFFANYLITQYRQVRTVKQPP"
     gene            complement(928..1986)
                     /gene="thiL"
                     /locus_tag="VNV63_04295"
     CDS             complement(928..1986)
                     /gene="thiL"
                     /locus_tag="VNV63_04295"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HXC61874.1"
                     /translation="MELRQLGERGLIHRIQTSLPSPARSGLILGIGDDAAVIRMAPGK
                     SLLLTTDLLVEGVDFDLALCSYHQIGYKALAANLSDIAAMGGLPRYFLVSLALPSLTS
                     VSAVDQLYRGMRRLAKRFEVALIGGDTSSSSRGLFLNLVVAGEIEPTRLVRRAGARIG
                     DQIFVTGRLGDSRAGLEILKLHASSHHKSQVKKNGAGKRAASNLQLETLINRHLYPMP
                     RLREGQLIASKGLATAMMDLSDGLASDLRRLCESSQVGAVINLSSLPVSSSLSRYAKM
                     RGREPSEYALIGGEDFELLFTVSSSRSNRLIKLQQKGQLKVTQIGQIRPRREGIRLIT
                     KGGRLKTLKAEGYEHFRT"
     gene            complement(2001..>2600)
                     /locus_tag="VNV63_04300"
     CDS             complement(2001..>2600)
                     /locus_tag="VNV63_04300"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HXC61875.1"
                     /translation="IYIEATTMKGKGNLTLTGHLGDVMKESAQAALSYVRSRDKTLGI
                     KTDVFSKYDIHVHVPAGAIPKDGPSAGITMACALASALTGTPLRRDVAMTGEVTLRGR
                     VLPIGGLKEKILAARRAGIQTVILPKRNEKDLEEVPKHVRRGLNFVFAETMDDVLKVA
                     LKHNHNRVGQGEKPVSEHRAKRRKSLISVAASARQKSLA"
BASE COUNT          616 a          724 c          666 g          594 t
ORIGIN      
        1 gatgaagcaa acccgctgga gtccgggctg gacatcattg cccaacctcc cggcaagcgt
       61 ttccggaata tctcacttct ctccggcggc gagagggcct tgaccgcgat cgcgctcctg
      121 ttctcaagtt ttttgatcca tccaagcccc ttctgtgtct tggacgagat cgatgcccca
      181 ctcgatgagg agaacaaccg acgcttcttg accgtcctaa agcgtttgac ggaccgttct
      241 cagtttatca tcatcactca cagcaaacgt acgatggagg cggccagcgt cctctatggt
      301 gtgacgatgg gagagccggg tgtctcgaag ctggtctccg tacacctcga aaaacattca
      361 ccgacagaga cagattccag cgtcaaagat caagctgtcc tgcagttgtg aagacttgca
      421 ccttaatcag ggcggctgtt tgacggtccg cacctgccga tactgagtaa taagatagtt
      481 tgcgaaaaaa taggcgatcg cggatgcgat gaccccgaca agggtcgttc ctaaaacaaa
      541 tgggatgaga tagggcttta actgagacat gaagttcagc atggtcaggt gcggccaatc
      601 tatcggcggg accgtacgct tcatcccatt aaactccatc ccataaagct tcaggcccaa
      661 ccagagacag ctcccgaaaa taggcccaaa agtccaggga ttgttgagca gtgaacccaa
      721 aagcaacgcg atcgtgttta gccgaaacag ccatgcaaag agaaaaacca tgccggtgtg
      781 aagtccgatt aacggggaga aggcgatata gatccccaga gcaaaagcga ccgcaatccg
      841 gtggggcgtg tcattcaagt gcagtaattt tctgaactgc tctttgacct tcttgatcat
      901 tgtctttaaa aaattagcag gattgtttta tgtacgaaaa tgctcatacc cctccgcctt
      961 caaggttttc agccttcctc ctttcgtgat cagcctaatc ccttcacgtc tgggtcggat
     1021 ctgtccgatc tgagttacct tcagctgccc tttttgctgg agcttaatga gacgattgga
     1081 ccgtgatgac gaaactgtaa ataacaactc gaaatcctcg ccgccgatga gggcgtattc
     1141 cgaaggttct cttccccgca tcttggcata gcgagacaag gaagacgaaa ccggaagtga
     1201 agagagatta atcaccgcac cgacttgact cgactcgcag agacggcgca gatcgcttgc
     1261 cagcccatcg gacaaatcca tcatggccgt tgcgaggccc tttgatgcaa taagttgccc
     1321 ttctctcagg cgcggcatcg ggtagagatg acgattgatg agtgtctcaa gttgcaggtt
     1381 cgatgctgca cgcttgcctg ccccattttt tttaacttgt gacttgtgat gtgatgaagc
     1441 gtgtaacttg agtatttcca accccgctct tgaatcgccc aagcggcctg tcacgaaaat
     1501 ctgatccccg attcgggcac ccgctcttcg aaccagtctg gtcggttcta tctcacccgc
     1561 gaccaccaga ttgagaaaga gccctcggct ggacgaagag gtatccccgc cgatcaaagc
     1621 gacctcgaac cgtttagcga ggcggcgcat cccacgatag agctgatcca cggcggatac
     1681 cgaagtcaga gaaggcaacg ccagggagac aaggaaatag cgggggagac cgcccattgc
     1741 agcgatatcg cttaaattcg ccgcaagggc cttgtatccg atctgatgat atgaacagag
     1801 agcgagatca aaatccacac cctcgaccaa caggtcggta gtcaacaaca gagactttcc
     1861 aggagccatc cggataactg cggcatcatc gcctatgccg aggatgaggc ccgatcgtgc
     1921 gggggaagga aggcttgttt gaatccgatg gatcaggccc cgctcaccca gttggcgtag
     1981 ttccatccgc tggcttcgct ttacgccaag gatttctgtc tggcagaggc tgcgactgag
     2041 atcagagatt ttctccgttt tgcccgatgc tctgaaacag gtttttctcc ttgtccgacc
     2101 ctgttatggt tatgcttaag ggccaccttg agcacgtcat ccattgtctc ggcaaaaaca
     2161 aaattcagcc cccggcgcac atgtttaggc acctcttcaa ggtccttctc attgcgttta
     2221 gggagaatta cggtttgaat cccggcgcgc ctcgcggcca ggatcttctc cttcaaaccc
     2281 ccgatgggga gaacgcgtcc cctcaacgtg acttcccccg tcatggccac atcccgacga
     2341 agcggtgtgc cggtgagggc cgaggccagg gcacacgcca tggtgatccc tgcggaaggg
     2401 ccatctttcg gaattgcccc tgccgggaca tggacatgga tatcgtattt gctgaataca
     2461 tctgtcttaa tcccaagcgt cttgtccctc gaccgtacat agctcaacgc cgcctgggcg
     2521 gactccttca tcacgtcacc gaggtgaccg gttagggtca ggttcccttt tcccttcatc
     2581 gtggtcgcct cgatataaat
//
LOCUS       DATMKH010000356         1366 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_808079,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000356 DATMKH010000000
VERSION     DATMKH010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_808079"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            294..722
                     /locus_tag="VNV63_04305"
     CDS             294..722
                     /locus_tag="VNV63_04305"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HXC61876.1"
                     /translation="MFGFGHFKGGRRGWLRPWILSIVSRSPKNGAEIMDEIEKMSWGG
                     WRPSPGSIYPLLEEMTKDGLIQKKDDGRYEITEGGKGETDWPFGMPFGNRPHGVDQML
                     SEINGYIMYFEDLSRTDRSKITQYQSSIRDLAARLSKLGE"
     gene            724..912
                     /locus_tag="VNV63_04310"
     CDS             724..912
                     /locus_tag="VNV63_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61877.1"
                     /translation="MTIHRAPIKEGDMVYVMDTKVRGKVLGRLKYLVLLELEGTHQMI
                     KEHRGNLKLIHPRLEAKF"
     gene            928..1299
                     /locus_tag="VNV63_04315"
     CDS             928..1299
                     /locus_tag="VNV63_04315"
                     /inference="COORDINATES: protein motif:HMM:NF021378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HXC61878.1"
                     /translation="MGLFFSPFRWLFLIFGIIVLAVIGITIVSTIFFGFALGHSLFFA
                     PRFFPFFGFPFLGFGFLIFGLLIFGFFRFLFYPYRRRGFYGNRWYGYDSAMDSLRQRY
                     ARGEITRDQFEQMAGDLEKHR"
BASE COUNT          350 a          329 c          350 g          337 t
ORIGIN      
        1 caaagacgat ccccagaaac ataatcaaaa tcccggcgca aatcaaggaa aggctaaaca
       61 tcgccatcgg catcgaataa tcttcttcgg taggagtttc cggactgtgt tccatctagc
      121 aggccgctct ccctcgttcc tgaacgccgg atagttcccg gccgctctga cacggcacgt
      181 gtccaagcgt gaaacgggta gaaatagtat tccaatgtcc atctattgga acagaaccag
      241 atgggaatcc ttatacggct ccgatatcga ataaccgata tcggtaaagt cacatgtttg
      301 gctttggaca ttttaaaggc ggaaggcggg gttggctaag accctggata ctctcaatcg
      361 taagcagatc gccgaaaaac ggcgcagaaa taatggatga aatcgagaag atgagctggg
      421 gaggctggcg cccttctccg ggttcaatct atcctcttct cgaggaaatg acaaaggacg
      481 ggttgatcca gaagaaggat gatggaaggt acgaaataac agaaggggga aaaggcgaaa
      541 ctgactggcc ctttggtatg ccttttggaa accgcccgca cggagtcgat cagatgctct
      601 ccgagattaa cgggtacatc atgtacttcg aagatctttc gaggaccgac aggtcaaaga
      661 tcacgcagta ccaatcctcg attcgcgatc tcgcggcaag gctctcaaag ctgggtgagt
      721 aagatgacga ttcatcgcgc cccgataaag gaaggagaca tggtatacgt catggatacc
      781 aaggtccgag gcaaggtgct cggcaggctg aagtacctcg tacttctgga gctcgaggga
      841 acccaccaga tgatcaagga gcatcgcggg aatctaaagc tgatccaccc gagactggaa
      901 gcgaaattct agggatgatg gcataaattg ggtttatttt tctctccgtt tagatggctt
      961 ttcctgatat tcgggataat agtactcgcg gttattggaa ttacgatagt ctcgacgatt
     1021 ttcttcgggt ttgctttagg gcattctctt ttcttcgctc caaggttctt tcccttcttt
     1081 ggattcccgt tcttaggctt cggcttcctg atttttggac tgctgatctt cgggtttttc
     1141 aggttccttt tctatcccta caggagacgc ggattctacg gaaacagatg gtacggatac
     1201 gactccgcaa tggactcttt aaggcagcgc tatgcccgcg gcgagattac tcgcgaccag
     1261 ttcgagcaga tggcgggcga cctcgagaag caccgctaaa ctccatctgg ccctcttttt
     1321 tcccggccag taactctttt ccgattactg acgtaaaaac tcaaat
//
LOCUS       DATMKH010000357         4030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1449849, whole genome shotgun sequence.
ACCESSION   DATMKH010000357 DATMKH010000000
VERSION     DATMKH010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4030
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1449849"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(615..1154)
                     /locus_tag="VNV63_04320"
     CDS             complement(615..1154)
                     /locus_tag="VNV63_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61879.1"
                     /translation="MDILEKYSLADWVYGVVTSLIAAAFFTAIVFTLRWFWGWIRNSS
                     AEYRRTDQRNRIIKIFIYRRYIGAANVFSLSRGHFFVISHCLQSFIAGVAIIAMGAIL
                     SWVMNMPIAFYMFLGLAIWMFIDATTWLDTRWSEKALEQLDEQALSEASAILGEPVDE
                     VRNQVTQPNVLTTHSAGHT"
     gene            complement(1217..1789)
                     /locus_tag="VNV63_04325"
     CDS             complement(1217..1789)
                     /locus_tag="VNV63_04325"
                     /inference="COORDINATES: protein motif:HMM:TIGR03033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HXC61880.1"
                     /translation="MSSFVIVEFEQGTHEWREWRHKGIGASDAPAIMGENRFKSAAQL
                     LQEKRGPVRSSAQNQAMALGTQLEPEARRRYIVKTGREVRPLCLQSTRYDWLRASLDG
                     FAINHDSVVEIKCGQSVYRRAWESRSVPDYYYGQMQHILAVTNLDLLDFWCYWPGCPE
                     LLLPVERDDAYIERLLNTELDFWNRVQRNA"
     gene            complement(1898..2503)
                     /gene="ruvA"
                     /locus_tag="VNV63_04330"
     CDS             complement(1898..2503)
                     /gene="ruvA"
                     /locus_tag="VNV63_04330"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HXC61881.1"
                     /translation="MIATITGRLIHKSPAYLIVDAHGVGYQIFTPLSSFYHLPEIKET
                     VMLNTYTHVREDALQLFGFLTMEEKDLFLLLITISGIGPKLALNILSGMPVAELVSAV
                     RQGDVARLCSIPGIGQKIAARLALELKEKIAAIKTAEVETVSGVSEERRQMIEDALSA
                     LVNLGYNRAVAKEAVLKISLAHEGELSVGTLLKESLKRLSR"
     gene            complement(2500..3018)
                     /gene="ruvC"
                     /locus_tag="VNV63_04335"
     CDS             complement(2500..3018)
                     /gene="ruvC"
                     /locus_tag="VNV63_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HXC61882.1"
                     /translation="MRVLGVDPGTVVTGYGVIDEEDQRLFFVASGGISTSSKFSISKR
                     LKKIFDELTVAIEDYQPHAVVVENTFVAKNMQTALKLGQARGVALLAAEQAGLPVFEY
                     TPMQVKLAVTGYGAASKVQVQNMVHKLLKLSQIPDSHHVADALAAAICHLHSAKMREL
                     ANASPSEIGRRA"
     gene            complement(3023..3775)
                     /locus_tag="VNV63_04340"
     CDS             complement(3023..3775)
                     /locus_tag="VNV63_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HXC61883.1"
                     /translation="MGGHSHWATTKHKKAAVDAKRGKVFTKIIREITTAARIGGGDPD
                     GNPRLRAVMLKARDQNMPQDNIKKAIQRGTGELPGVVYEETTYECYGPGGVAMLVEIM
                     SDNKNRTVSEVRHILTKYGGSISGSGTVAWIFSRKGYITVEKTKSDEEKLMTLALEAG
                     AEDMKTGERYFEVLTVPSDLEKVKKALEKNRIPMAVSEVTMVPQNYVHLEGRDAEQML
                     KLMEEMEDHEDVQNLYANFDIPDEVMDKIAAR"
     gene            complement(3781..>4030)
                     /locus_tag="VNV63_04345"
     CDS             complement(3781..>4030)
                     /locus_tag="VNV63_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61884.1"
                     /translation="ARGVGVVETLARICKLVLQKVKRSLETLAPGWDEDPVTKFVRSK
                     EAMQPTVIEGLWSRRGESAVEKEEKALRHRDIFGRSWM"
BASE COUNT          898 a         1132 c         1010 g          990 t
ORIGIN      
        1 ctttatagtg cagattctga actaagggtg caatagaaag tttgagttcc cgcagcgctt
       61 tatcggccgc tgtaacctcc ggcaggcgtt ccgcattgat tcggttgaga agatactcgc
      121 gctcatgtac tttacacatc gttccgctaa aacgctgaac tttagtgagg cgactgctga
      181 gattatctgt gaatagggta gttcccttga tatccgtaca cttccatttt cctattaccg
      241 agcgcacccg agagccatcg ggctctattt ctcgaacttc cgtgtcggcg aagcaccagt
      301 aaagccgtct cttgtagaaa gtaatccaca gcgcatcgca cccaagtgta taaaagtctt
      361 gggttcgctt tacaatcgtt gtgacctgtt tgggacgctc cttcctccag aacttccgaa
      421 gcacctccca ttttccctca aggcatgctt gatgatgggg attggagaat ccaaagcgga
      481 cgattggcct tgggctatca atgcatctct tttcccatga cccacggctt ccgagcttga
      541 tgaaacggac ctctttcgcc gttatggtgt tagctctcat tgattcctga tgcccaagtt
      601 aaccggcggc cggtttatgt atgtccggca gaatgagtgg ttaggacgtt tggttgagtg
      661 acctgatttc gcacctcatc gaccggttcg cccaagattg cggaggcctc agagagtgcc
      721 tgctcatcga gttgttcgag tgccttctcc gaccagcgag tatcgagcca agttgtggca
      781 tcaatgaaca tccatatagc tagccccagg aacatataaa aagcgatcgg catgttcata
      841 acccaactaa gaatagctcc catcgcaata attgcaactc ctgcaatgaa cgattggaga
      901 cagtgagata tgacaaagaa gtgtcctcgg gatagcgaaa acacgtttgc tgccccgatg
      961 tagcgccgat atataaaaat cttgataatg cgattcctct gatcagttcg gcgatactcc
     1021 gctgaagaat ttcttatcca gccccaaaac caccgaagtg tgaaaacaat tgcagtaaag
     1081 aaagccgcag cgatgaggct tgtgacaact ccataaaccc aatcggctag agagtatttt
     1141 tccagaatat ccattggttg atggtgtcaa ggagagagat tagctcgata atcacttcag
     1201 ggactctttc agcttcttac gcgtttcgtt ggacacggtt ccaaaaatcc agctctgtat
     1261 tcaggagacg ttcgatgtag gcatcgtctc gctcaacagg cagaagcaat tcggggcagc
     1321 ccggccagta acaccaaaaa tcgagcaaat caagattagt aaccgccaag atgtgttgca
     1381 tctgaccgta gtaataatcg ggaacggaac gagattccca ggctctccgg tagactgatt
     1441 ggccgcattt gatttcgact accgaatcgt gattgatggc gaagccatcg agactcgcgc
     1501 gcagccagtc gtatcgggtg ctctgaaggc aaaggggcct gacttctctg ccggtcttga
     1561 cgatgtagcg cctgcgggcc tcaggctcaa gctgggtgcc cagagccatc gcttggttct
     1621 gggcagagct tcgtacgggt ccgcgcttct cttgtaatag ttgtgcggcg ctcttgaagc
     1681 ggttctcgcc catgatggcc ggagcatctg acgctccaat gcccttgtga cgccactcgc
     1741 gccattcatg cgttccttgt tcgaattcaa cgatgacaaa gctggacatg ttcactaaac
     1801 cctccatgcg ctatggacgt cacataaagt gggatatcaa caccaatact tactatatta
     1861 acgcccataa tatcccataa aatctccatt tgaaaagtta ccgagacaac cgtttcaggg
     1921 actctttcag cagggtccca accgagagtt cgccttcatg ggccaggcta atcttaagaa
     1981 ccgcctcttt ggccacggcg cggttatatc ccaggttcac aagggccgag agcgcatcct
     2041 ctatcatctg ccgccgctct tcgcttactc cagagaccgt ctccacttcc gccgtcttga
     2101 tcgccgcaat cttttctttg agctccagag cgagacgcgc cgcgatcttc tggccgatgc
     2161 ccgggatcga acagaggcgg gccacgtccc cctgccgcac cgccgatacc agttccgcga
     2221 ccggcatgcc ggacagtata ttcaaagcca gcttcggtcc gatgccggag atcgtaatta
     2281 agagcagaaa gagatccttt tcctccatcg tgagaaaccc gaagagctga agggcgtctt
     2341 cccggacgtg ggtgtaggta ttgagcatga cggtttcttt gatttcgggg agatgataga
     2401 aagatgagag cggtgtgaaa atctggtaac ctacgccgtg ggcatctacg atcaaatagg
     2461 caggggattt atgaatcaat cgcccggtga tcgtggcgat catgcccgcc ggccgatctc
     2521 ggatggggag gcattagcca gttcccgcat cttggccgaa tgcaggtggc agatcgccgc
     2581 tgccaaggca tccgccacat ggtgggaatc cgggatttga ctgagtttga ggagcttatg
     2641 gaccatgttc tggacctgaa ccttacttgc cgctccgtag ccggtcacgg ccagcttcac
     2701 ctgcatgggt gtgtattcaa acaccggcag gcctgcctgc tccgccgcaa gtaacgccac
     2761 cccgcgggcc tgtccgagct tcagcgccgt ctgcatattc ttggcgacaa aggtgttctc
     2821 caccacgacg gcatgaggtt gatagtcctc gatggcaacc gtcagctcat caaatatttt
     2881 tttgagccgt ttcgagatgg aaaacttcga ggaggtgctg atcccgccgg aggcgacgaa
     2941 aaacagccgc tgatcctcct catcaatcac accataaccg gtcacgactg tgccgggatc
     3001 aacaccaagg acacgcatgg gtttatctcg cagcgatctt gtccattact tcatccggga
     3061 tgtcgaaatt ggcgtataga ttctggacat cctcatgatc ctccatctcc tccatgagct
     3121 tgagcatctg ctccgcgtcc cggccttcga gatgcacata attctgaggg accatcgtta
     3181 cctcggacac ggccatcggg atccggtttt tctctaaggc tttttttact ttctctaaat
     3241 cggaaggaac cgtcaggact tcaaaatacc gctctccggt cttcatgtcc tctgcaccgg
     3301 cctctaaggc cagcgtcatg agtttttctt catccgactt cgtcttctcc accgtgatgt
     3361 agccctttcg ggaaaagatc caggccacag tccccgaccc gctgatgctg ccgccgtatt
     3421 tggtcaagat gtgacggacc tcggagacgg tgcggttctt gttatccgac atgatctcga
     3481 ccagcatcgc gactccgccg ggtccatagc attcataggt cgtctcttca tacaccacgc
     3541 ccggaagctc acccgtgccg cgctggatcg ccttcttgat gttatcctgc ggcatgttct
     3601 gatcgcgcgc cttcaacatc acggcacgaa gacgcgggtt gccgtcgggg tcgccgccgc
     3661 cgattcgtgc agcggtcgta atctcgcgga tgatcttggt gaagaccttt ccgcgctttg
     3721 cgtctaccgc ggccttctta tgtttggtgg ttgcccagtg agaatgtccg cccattttgg
     3781 ttacatccag ctccggccaa aaatatctcg gtgcctcaat gccttctctt ctttctccac
     3841 tgccgactcc ccgcggcggc tccagagtcc ttcgatcacg gtcggctgca tggcttcttt
     3901 agagcgaacg aatttcgtta ccggatcctc atcccaaccg ggggccagtg tttcaagaga
     3961 acgtttgacc ttctgcaaga ccagtttgca gatccgagcc agggtttcaa ccacgcccac
     4021 accacgcgca
//
LOCUS       DATMKH010000358         1300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4644349, whole genome shotgun sequence.
ACCESSION   DATMKH010000358 DATMKH010000000
VERSION     DATMKH010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1300
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4644349"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="VNV63_04350"
     CDS             <1..269
                     /locus_tag="VNV63_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61885.1"
                     /translation="VVESSKIAATLRVEGRITGPWVEELRTACNLDTFSDEVQLSLEL
                     ADVSFADPAGIALLRELRNRGVGLIGTTPFLAEQLKDGTSYLKF"
     gene            354..1172
                     /locus_tag="VNV63_04355"
     CDS             354..1172
                     /locus_tag="VNV63_04355"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HXC61886.1"
                     /translation="MLEQIVCRKRLLPAGTLRCVQRNIPIPEPQSDVSKMVALICHDL
                     RLPLTAILANAEFLTQSDISETERSELYQEIRWSIERMNELVTSLFECSKGRDTLRPA
                     VRSIVDTVKRAIRMTSVRKEFRHIMIKHHHKGLAVGWFDSNRLERVVANLVLNACEAV
                     SPDSGRIVVTTTGNQACLQIGVWDNGPGIPPEIQDSVFQPFVSYGKSEGSGLGLAIVK
                     KIVEDHGGEIYVDGRSGTGTLFKITIPFRHSGGSNPAHVCRSDLLHAHVKKRGA"
BASE COUNT          316 a          336 c          357 g          291 t
ORIGIN      
        1 cggtcgtcga atcatcaaag attgcagcaa ctcttcgggt agaaggccgg ataacgggtc
       61 cctgggtcga ggagctacgc accgcatgca atctggatac tttttccgat gaggttcagt
      121 tgtccctcga actggcggac gtctcattcg ccgacccggc ggggatcgct ctcctcagag
      181 aactgcggaa ccgcggcgtc ggtctgatcg gcacgacgcc gtttttggcc gaacagctca
      241 aagatgggac ttcatatctc aaattctagg gtacgaggat cgcatgccaa cggccggaag
      301 ggatcccctc cccctctgaa cttcggccca tagcagacgg taggaggttt tcaatgctgg
      361 agcaaattgt gtgcaggaag cggttattgc cggccgggac tttgcgatgc gttcaaagaa
      421 atattccaat tccggagccg caatccgacg taagcaagat ggtggccctc atctgccacg
      481 atcttcgcct tcctttgacc gccattctgg cgaatgcaga atttctcacc caatcagata
      541 tcagcgaaac agaaaggagc gaactttacc aggaaatccg ttggtccatc gagcggatga
      601 atgaactggt aacttcattg tttgagtgct ccaagggtcg tgacactctt cgccccgctg
      661 tccgaagcat cgttgacaca gtcaagcgtg cgatccggat gacaagtgtg agaaaggagt
      721 tccgccacat catgatcaag caccatcata aaggcttggc cgtgggctgg ttcgattcca
      781 accgtctcga acgggtcgtc gccaacctcg tcttgaatgc ttgcgaggcg gtttctccgg
      841 actcaggacg gatcgttgta accacaaccg gaaatcaagc ctgcttgcaa attggcgttt
      901 gggataacgg ccccggaatc ccgccggaga tacaggactc cgtctttcaa ccatttgtga
      961 gctacggaaa gtccgaaggc agcggactgg gcctcgcgat agtaaagaag attgtcgagg
     1021 atcatggcgg ggagatctac gttgatggga gaagtggcac tggaacttta ttcaagatca
     1081 cgattccatt tcgccattct ggaggaagca atccagctca tgtctgtagg tccgatctac
     1141 tccacgcaca tgtcaagaaa cgtggcgcgt aatgattaag accaagtcgc tatcaattat
     1201 cgatgttgag taatcatgcc tcaggcggtc aggagggccc attcatgaca cgtgaggaac
     1261 atcagacaag tcaggaatac atgtggcgag cacttttcgg
//
LOCUS       DATMKH010000359         1249 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1131975, whole genome shotgun sequence.
ACCESSION   DATMKH010000359 DATMKH010000000
VERSION     DATMKH010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1131975"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VNV63_04360"
     CDS             complement(<1..224)
                     /locus_tag="VNV63_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HXC61887.1"
                     /translation="MEQIRVELGERSYTIMIGSRMLERIGEELEPLEIGKKIGVVTNA
                     KVGRLYGNTIVRNLRQNGFDPLILQVPDGE"
     gene            complement(205..747)
                     /locus_tag="VNV63_04365"
     CDS             complement(205..747)
                     /locus_tag="VNV63_04365"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HXC61888.1"
                     /translation="MRNIVLIGFMGTGKTVVGKRLAARLGYRYVDTDMIIEEQTGCTI
                     AEIFKNRGESYFREVERSVIQKVVRSKGSVISTGGGAVVDPENLALLKKNGLVICLKS
                     SPEKIMERTGRKKNERPLLSGKEPLEETKRLLELRAPYYEQADWMVDTSKKTVAQVVE
                     IVVATIEQKLKAGDIGADPS"
     gene            complement(744..>1249)
                     /locus_tag="VNV63_04370"
     CDS             complement(744..>1249)
                     /locus_tag="VNV63_04370"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HXC61889.1"
                     /translation="NPPIGLGSYVQWDRRLTARLAMALMSIQAMKGVEVGLGFESARR
                     FGSEVHDEIFYDKKARRNFYRKTNRAGGIEGGMTNGEDIVLRTAMKPIATLYKPLQSV
                     DLKTKEPFKATIERSDICTVPAASVIGEAVVAFETASAMVEKFGGDSLQEMKHNFKGY
                     MDYVRQR"
BASE COUNT          268 a          389 c          273 g          319 t
ORIGIN      
        1 tgctcaccat ccggaacctg gagaatcaac ggatcaaaac cgttttgccg gaggttgcgg
       61 actatcgtat tgccatataa gcggcccacc ttcgcattgg tgacaacgcc gattttcttt
      121 ccgatctcca agggttccaa ttcctccccg attcgttcca gcatccgact cccgatcata
      181 atcgtgtagg accgctcacc caattcaact cggatctgct ccaatgtccc ctgcctttag
      241 cttctgttcg atggttgcca caacgatttc tacaacctgg gcaaccgttt ttttagaagt
      301 gtccaccatc cagtccgcct gttcatagta cggcgctctt aattcgagca gccgcttggt
      361 ctcttccaac ggctcttttc ccgagagtaa cggccgttcg ttttttttcc tcccggtccg
      421 ctccatgatc ttctcaggcg aagacttaag acagatgacc agcccgtttt tcttgagaag
      481 agcaaggttc tccgggtcga caaccgcacc tccacccgtc gagatgacag atcctttaga
      541 ccgcaccact ttctggatta cggaacgctc tacttcgcga aaataggatt cgccgcgatt
      601 tttaaatatc tcggcgatcg tacatcccgt ctgctcttcg atgatcatat cggtatccac
      661 gtaacggtag cctaacctgg ccgccagcct cttccccacc acagtcttcc ctgtgcccat
      721 aaaaccgatg agaacaatgt tcctcagcgc tgcctcacgt agtccatgta acctttaaaa
      781 ttatgtttca tctcctgaag gctgtcgccg ccgaactttt caaccatcgc agatgcggtc
      841 tcaaaggcga cgacggcttc accgatcaca ctcgcggcgg ggacggtaca gatgtcggaa
      901 cgctcgatcg tggccttgaa cggttccttg gtcttaagat ccacgctctg tagcggttta
      961 tacagtgtcg caatcggctt catggcggtc cgtaggacaa tatcctctcc attggtcatg
     1021 ccgccttcga tgccgccggc acggttggtc tttcgataaa aattacgacg tgctttctta
     1081 tcgtagaaga tctcatcatg cacctcggat ccaaaccgcc gggccgactc aaaacccagc
     1141 ccgacttcca ctcccttcat tgcctgaatg ctcatcaagg ccatggccaa acgcgcggta
     1201 agacggcggt cccactggac atagctgccc aatccaatcg gcggattcg
//
LOCUS       DATMKH010000360         1067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2089751, whole genome shotgun sequence.
ACCESSION   DATMKH010000360 DATMKH010000000
VERSION     DATMKH010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2089751"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="VNV63_04375"
     CDS             complement(<1..662)
                     /locus_tag="VNV63_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61890.1"
                     /translation="MTLGHLDRRFSLSSLFKRESGWGLLLILYGLSASGLWSNRTGIS
                     WWHGLWIGCFVVLGLRFGLVGLQGLGLDKKSRLQLVLIGILSVNTLVQVTGGIASPFL
                     PIYLLATVLLPLTMSEVETIIGVGGIFALEGVNLALRLSIENTALIRAGVMGILLFLI
                     PILIWRTVRSFRGTASQLRTSLDRIQGGLQMVEPLPEPGSDGDLRPISEDYKQDQAVI
                     ALA"
     gene            complement(659..919)
                     /locus_tag="VNV63_04380"
     CDS             complement(659..919)
                     /locus_tag="VNV63_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61891.1"
                     /translation="MIEMKQLELKIQAVKKHLQESKKQAESPSTPPKLRQAQKRLRRL
                     QRKRRALAVRQQRVAKKAVSKKATEPAKQEAGEKAVGGQPPG"
BASE COUNT          238 a          299 c          277 g          253 t
ORIGIN      
        1 gctaatgcga tgacagcctg atcctgttta taatcttccg agatcggtcg cagatcgcca
       61 tcggaaccgg gttctggcaa gggttctacc atctggagac cgccctggat ccgatcgagt
      121 gaggtgcgta attgtgacgc ggtcccacga aacgaccgga cggttcgcca gattaagatc
      181 gggatcaaaa aaaggaggat acccatgacg ccggcacgga tcagcgcagt gttctcaata
      241 gagagacgaa gcgctaggtt tacgccttcc aaagcaaaaa ttccgccaac ccctattatg
      301 gtttcaactt cgctcatcgt gagcggaagg aggaccgttg caagcagata gatcggcagg
      361 aaaggtgagg caatgccgcc cgtgacctgg accagggtgt taacagagag tatcccgatc
      421 agaaccaact ggagcctgct ctttttatcc agcccaagcc cttgtaatcc tactaaaccg
      481 aaacgcaaac ctaatacgac aaagcaaccg atccagagcc catgccacca actgatcccc
      541 gtgcgattgc tccaaagtcc actcgcggag aggccgtata aaatcagcaa cagcccccag
      601 ccgctttctc gtttgaaaag cgacgagaga ctaaaacggc ggtcgaggtg cccgagagtc
      661 atccgggcgg ttggcctcct actgctttct cgcccgcttc ttgcttcgcg ggttcggtcg
      721 ctttcttgga cacggccttc ttggccaccc gctgttgcct gacagcgagg gcacgacgct
      781 ttctttgaag ccttcggagc cgtttttgtg cttgacggag tttcggaggc gttgatgggc
      841 tctccgcctg ttttttcgac tcctgcagat gttttttcac cgcctgaatc ttgagctcaa
      901 gctgtttcat ctctatcatt tattttcctt tctccatttt tatgggtccc gtcgagtgac
      961 gaaggtctca agcttcgcat cggaggcaag tctgcgtgca acttcttcgg ccatcagacg
     1021 gctggagtaa tttccgaccc ggacgcgata aagctgctga ctgttcg
//
LOCUS       DATMKH010000361         1051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4645142, whole genome shotgun sequence.
ACCESSION   DATMKH010000361 DATMKH010000000
VERSION     DATMKH010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4645142"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            312..>1051
                     /locus_tag="VNV63_04385"
     CDS             312..>1051
                     /locus_tag="VNV63_04385"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXC61892.1"
                     /translation="MTKPKIALRYSCLLLTLLLAGGCSRSAKSYLQSGRKYFDSGKYE
                     DAIILYKKAIQKDPKSGEAYYRMALAELKLGKIPDAYQALSAASSLSTDNNEIQSVFA
                     DFCFEIYLLDRNHPKGFYDRVSKISQDMLAKDKNSYDGLRLKGYLAFEDRKPADAIAA
                     LRQADQVRPMQAPVVYPLVRALIQDHQEAAAEKLAQDFLQKNKTFGPIYDVLFGLYGR
                     SGRQQEAENILKSKVDNNPKQASYVLNLA"
BASE COUNT          265 a          288 c          269 g          229 t
ORIGIN      
        1 ctacgatcat tggctggaag attcacggat cgagttttat ccggagccga gcggactgga
       61 agcagggctc cgggaagcga cagcgccttt cgcaaataaa aatgtcgcct aagtggatcg
      121 gcgattgcta tctgtatgcg aagcactctc atgccacgct gttcactttc gacaaggcgc
      181 tgcatcatct tgcacgcaaa caacactgct ccgcaattga accgcgttga gccttggata
      241 gtaccagtac cgttctctca agcaacaccc gaagaccccg agttatacta gtcaggccgg
      301 gagtttctga cgtgacgaaa cctaaaatag ctctacgata tagctgcctg ttactcactc
      361 tgctcttggc cggcggctgt tcccgcagcg caaaaagcta cctgcaatcc ggaaggaaat
      421 acttcgattc cggaaagtac gaagatgcga taatcctcta caagaaggcc atccaaaagg
      481 atccaaaatc gggggaagcg tactatcgga tggcgctcgc ggagctgaag ctgggcaaga
      541 ttccggatgc ctatcaggct ctctcggcgg catcctccct ctccaccgac aacaatgaga
      601 ttcaaagtgt attcgccgac ttttgctttg agatctatct gctggaccgg aatcatccga
      661 agggcttcta tgacagggtt tccaagatct cccaggatat gctggctaag gataagaatt
      721 cctatgacgg cctgcgcctg aaaggttacc tggccttcga ggaccggaag ccggcagatg
      781 caattgctgc actccgccag gctgaccaag tgcgtcccat gcaggcacct gtcgtctacc
      841 cgctggtccg ggctttgatc caggatcatc aggaagcggc agccgaaaag ctggcgcagg
      901 atttcctaca aaaaaacaaa accttcggcc caatttacga tgttctattc ggtttgtacg
      961 gccgctcggg gcggcagcag gaagccgaga acatcttgaa gtccaaggtg gacaataatc
     1021 ccaaacaggc ctcttacgtt ctgaatcttg c
//
LOCUS       DATMKH010000362         2342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1766018, whole genome shotgun sequence.
ACCESSION   DATMKH010000362 DATMKH010000000
VERSION     DATMKH010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1766018"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="VNV63_04390"
     CDS             <1..441
                     /locus_tag="VNV63_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012462877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61893.1"
                     /translation="NSDFRAVLSRSGDSFELDDLKPGDLLFWSGTYKVDRDIPITHVM
                     IYLGKEKSTGNPVMVGSTDGRTYDGVRRYGVSVFDFKMPSGKPNKDDPVLTAKFEGYA
                     SIPGLREVDVASRNRANPEEGKQSDKETPNGKAKPTTNTMGDGD"
     gene            534..1805
                     /gene="serS"
                     /locus_tag="VNV63_04395"
     CDS             534..1805
                     /gene="serS"
                     /locus_tag="VNV63_04395"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HXC61894.1"
                     /translation="MLDIRLIRERPDFIRERLTTRSAELAQQVDEVLKIDSERRRLET
                     QLQQLNAERNKLSKEIGMLRSKKESSAEQEAKVRAIGEQIVYLNEAVAAADESQTKLL
                     LGLPNLPHAKAPIGADAGGNLEVRVWGEKPVFSFQPLDHVDLGTKLGLFDLERSVKIS
                     GAGFVCFTNLGARLHRGLIQFLLDLHTTEHGYVEICPPHLVRRECMVGTGQLPKFEED
                     AYGLEEGRTFLIPTAEVPLTNLYRDEILANGELPKYLVAHTPCFRREAGSAGRDTRGI
                     IRIHQFDKVELVKITDPETSYQELEKLTADAEKVLKRLGLHYRVIELCTGDLGFSMAK
                     TYDIEVWAPGQDRYLEVSSCSNAEDYQARRMNLKFKDVDGKNRFCHTLNGSGTALPRL
                     YVALVETYQQPDGSILIPEALRDYVGVKEIR"
     gene            complement(1864..>2342)
                     /locus_tag="VNV63_04400"
     CDS             complement(1864..>2342)
                     /locus_tag="VNV63_04400"
                     /inference="COORDINATES: protein motif:HMM:NF016086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HXC61895.1"
                     /translation="ILLPSGEQIGGNVDTVQWMAVLKSCSALEPYRKHYRGQVVPWKV
                     VEFLVKNQTFPRSILFCVDSLDFALHKITGVDRGDFQYKVEAERLSGKLLADLCFVTI
                     DEIFRTGLHEYLDRMQGRLIVISKAIYKEFCEWLEEGQEEEEQEEPQPNQFQEQKR"
BASE COUNT          560 a          593 c          662 g          527 t
ORIGIN      
        1 aacagcgatt ttcgtgcggt gctgagccga agcggggatt ctttcgagct cgacgatctc
       61 aagccgggcg atctcctgtt ctggtccggc acctacaagg tcgatcggga tattccgatt
      121 acgcacgtga tgatctatct gggcaaagaa aaatccaccg gcaaccctgt catggtaggt
      181 tccacagacg ggcgtaccta cgacggggtt cgtcgctacg gtgtcagcgt gtttgatttt
      241 aaaatgccaa gcggcaaacc gaacaaagat gatccggttc tgacggcaaa atttgaggga
      301 tacgcgagta tccccgggct gcgcgaggta gatgtggcgt caaggaaccg ggcaaatccg
      361 gaagagggca aacagagcga caaggagaca cccaacggaa aagcaaagcc gacgactaat
      421 acgatgggtg acggggatta gatcagtgcc actggcaacc ggcgggcgat cgatactccg
      481 cgatgatcga cgattttctt ttactcggtc gggaggcggt atagcgtccc gtcatgctgg
      541 atattcgtct gatacgagag agacctgatt tcattcgcga gcgcctgact acgcgcagcg
      601 cggaactggc tcaacaggtt gacgaagttt taaagatcga ttcggaacgg cgccggttgg
      661 agactcagct tcagcagtta aatgccgaac gaaacaagct gagcaaagag atcgggatgc
      721 tgcgatcaaa gaaggaatcc agcgccgagc aggaagcaaa agtcagggcc ataggtgaac
      781 aaattgtcta tttgaatgaa gcggtggccg ccgccgacga atcccagacg aagctattac
      841 tcggcctacc gaacctgcct catgcgaagg ctcccatagg agccgatgcc ggcggcaatc
      901 tcgaggtgcg cgtctggggt gaaaaacccg tctttagttt ccagccttta gatcacgttg
      961 atttgggaac aaagctaggc ctttttgatc tcgagcgttc tgtcaaaatc agcggtgccg
     1021 gctttgtctg ctttaccaac ctcggagcac ggctgcatcg tggtttgatc cagtttttgc
     1081 tggacctcca cacgaccgag cacggctatg tggaaatctg tccgcctcat ctcgtgcgac
     1141 gtgaatgcat ggtcggcacc ggacagttgc ccaagttcga ggaagacgcc tatgggctgg
     1201 aggagggacg aacttttttg attcccactg cggaggtgcc tttgaccaat ctttatcgcg
     1261 acgagatatt ggccaacgga gagttgccaa aatatttagt cgctcacacg ccttgttttc
     1321 gccgggaggc aggatccgca ggtcgtgaca cgcgggggat catccggata catcaatttg
     1381 ataaagtgga actggtcaaa atcacggacc cggaaacctc ctatcaggaa cttgagaagc
     1441 tgacggccga cgcagagaaa gtgctgaaga gacttggact gcattaccga gtgatcgagc
     1501 tttgcaccgg cgacctcggg ttcagtatgg cgaaaacgta cgatatagag gtctgggccc
     1561 ccggccagga ccggtatctg gaggtttcga gttgttcgaa tgcggaagat tatcaggcgc
     1621 gccggatgaa tctgaagttt aaggacgtgg acggaaaaaa ccgcttttgt cataccctga
     1681 atggctcagg gaccgctctt cctcgactct acgtggcgct ggtggagacc tatcagcaac
     1741 ccgacggatc gatcctgatt cctgaagcgc tcagggacta cgtcggagtc aaggagataa
     1801 ggtagttcgt ttgtaccggc caagcctttt ctgaccgaac ttctggaact gctgactcct
     1861 gattcatctt ttttgttcct ggaattgatt tggctggggc tcctcctgct cttcctcctc
     1921 ctggccttct tcaagccact cgcaaaactc tttgtagatg gccttgctga tcacgatcag
     1981 gcgtccctgc atcctgtcca ggtattcgtg aagtccggta cggaaaatct cgtcgatggt
     2041 gacgaaacag agatcggcca ggagcttacc ggaaaggcgc tctgcttcca ccttgtactg
     2101 aaaatctcct cgatcgactc cagtgatctt gtgaagtgcg aagtcgaggg aatcaacgca
     2161 gaagagaatt gatcgaggaa acgtctgatt cttgaccaga aattctacga ctttccacgg
     2221 cacaacctgg cctcgataat gcttccgata gggttctagt gcgctgcatg acttcagaac
     2281 cgccatccat tggacggtgt cgacgttacc gccgatctgt tcaccgcttg gcaacaggat
     2341 gt
//
LOCUS       DATMKH010000363         1371 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3053951, whole genome shotgun sequence.
ACCESSION   DATMKH010000363 DATMKH010000000
VERSION     DATMKH010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1371
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3053951"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1362
                     /locus_tag="VNV63_04405"
     CDS             <1..1362
                     /locus_tag="VNV63_04405"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HXC61896.1"
                     /translation="SKAMMVMVSEVQSKLSAPGGYNLDGRLYYQGSRAELQGLIEEIA
                     QVHGTDINIYDLDGNLQVSSNLFVYNKGILSKKMNPLAYYNLHQLNTIQFPNFEQIGG
                     ITYVSIYRPLRDDQGNAFAYLNIPSFTRQDELKEEISNFLVTIINLNAFIFLIAGTIA
                     LFLTNRITSSFLLISKKMQEVNLGQTNEEIEWNRNDEIGGLVKEYNKMVKKLEESAVA
                     LAKSEREGAWREMARQVAHEIKNPLTPMKLSIQYLQKAIDNNSANVKEMAGSVAKTLV
                     EQIDHLSKIASDFSQFANIGNPKKEVFDLHEMLYSLTSLYETTENLLFKWVPVHHRIM
                     VYADKTQLNRLFTNLLQNAMEACDSNSRRVISVSEEMGDESIIVKVTDNGEGIPAEMQ
                     SKIFIPNFTTKSSGTGLGLAMSKTIVEQAKGKIWFETMEGEGTTFYVELPILRATKPL
                     EAV"
BASE COUNT          352 a          290 c          447 g          282 t
ORIGIN      
        1 agcaaggcca tgatggtcat ggtgagcgag gtgcagagca agctgtctgc ccccggcggg
       61 tacaacctgg acgggaggct ttattaccag gggtcgaggg cggaactgca gggattgatc
      121 gaggagatcg cacaggtcca cgggacggat atcaatatct atgacctcga tgggaatttg
      181 caggtgtcgt ccaacctgtt cgtgtacaat aaggggatcc tcagcaaaaa gatgaatccg
      241 ctggcatact acaatcttca ccagttgaat acgatccagt ttcccaactt tgagcagatc
      301 ggggggataa cgtatgtgag catttaccgg ccgctgcggg acgatcaggg gaatgcgttt
      361 gcctatctga atatcccgtc cttcacgagg caggatgaat tgaaggaaga gatctcgaac
      421 ttcctggtga cgatcatcaa cctgaatgcg tttatctttc tgatcgcggg tacgatcgcc
      481 cttttcctga cgaaccggat aacgtcgtcc ttcctgctga tctccaagaa gatgcaggag
      541 gtaaacctgg gacagacaaa tgaggagatc gagtggaatc ggaatgatga gatcgggggg
      601 ctggtaaagg agtataataa gatggtgaag aagctggagg agagcgcggt agcactggcg
      661 aagagcgagc gggaaggggc gtggcgcgag atggcccggc aggtggcaca tgagatcaag
      721 aacccgctga cgccgatgaa gctgagcatc caatatttgc agaaggcgat cgacaataat
      781 tcggcgaatg tgaaggagat ggcggggagc gtggccaaga cgctggtgga gcagatcgat
      841 cacctgtcga agatcgcgtc cgacttttcg cagtttgcga atatcgggaa cccgaagaag
      901 gaggtcttcg atctgcacga aatgttgtat tcacttacgt ccttgtatga gacgacggaa
      961 aacctgttgt tcaagtgggt gccggtgcat caccggatca tggtctatgc cgacaagact
     1021 cagttgaacc ggttgttcac gaacctgctg cagaatgcga tggaggcctg tgacagcaat
     1081 agccggcggg tgatcagcgt gagtgaggag atgggggatg agtcgatcat cgtgaaggtg
     1141 acggacaatg gggagggaat accggcggag atgcagtcga agatcttcat acccaatttc
     1201 acgaccaagt catcggggac ggggttgggg ctggcgatga gcaaaacgat cgtagagcag
     1261 gcgaagggga agatttggtt tgagacgatg gagggggagg ggacgacctt ctatgtggag
     1321 ttgccgattt tacgcgccac caaaccgctg gaagcggttt aaaatcgcct t
//
LOCUS       DATMKH010000364         1254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4340341, whole genome shotgun sequence.
ACCESSION   DATMKH010000364 DATMKH010000000
VERSION     DATMKH010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1254
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4340341"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="VNV63_04410"
     CDS             <1..618
                     /locus_tag="VNV63_04410"
                     /inference="COORDINATES: protein motif:HMM:NF040246.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HXC61897.1"
                     /translation="KLQEHLSEFGASNIDELLAFIGYGKISAQQVINKLIPEPDLKEG
                     LADKIGKKLRIHDTGMKVRGVNDILIRLSKCCNPLPGDKIIGFVTRGRGLSVHTVECP
                     NIDELDYDRDRIVEVSWDTKDSRTHSVAIRVLTMDRPGMLASVSSALSSASSNISHAE
                     IITTEEKKAVLNFVVDVNDTKHLEKILKSIRQVDGVLEAKRVRKG"
     gene            637..1134
                     /locus_tag="VNV63_04415"
     CDS             637..1134
                     /locus_tag="VNV63_04415"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HXC61898.1"
                     /translation="MISRRGFFETVIAVVAGVVGLGLSIPLAGYSIWPALKKRETGWV
                     DLGPIEQLKSGEPKQLEFLATQMDGWLKTSITQWVWAVRHSDEEITVYSPICTHLGCG
                     YHWDSGEGQFACPCHGSIFSIDGKVLAGPAPRPLDTLPVKIENGRLFITYEQFKSGTK
                     EKVAI"
BASE COUNT          289 a          319 c          355 g          291 t
ORIGIN      
        1 aaacttcagg agcacttgag cgagtttgga gcctcgaaca ttgatgaact ccttgcattt
       61 atcgggtacg gcaaaatttc cgcccagcag gtgatcaaca agctgatccc cgagccggac
      121 ttaaaagaag ggctcgccga taagattggc aagaagcttc gcattcatga caccggtatg
      181 aaggtccgcg gggtgaatga tatactgatc cggctttcca aatgttgtaa cccgctgccc
      241 ggtgacaaga tcatcggttt cgtcacacgc ggacgcgggc tatcggtgca tacggtagaa
      301 tgccccaata ttgatgagct ggactatgat cgggatcgca tcgtggaggt ttcctgggat
      361 acgaaagatt cgcgaaccca ttcggtggcg atccgcgtgc tgaccatgga ccggcccggc
      421 atgctggcct ccgtctcatc cgccctctcg tccgcttcct ctaacatcag tcacgccgag
      481 attatcacca ctgaagagaa gaaggcggtt ctaaattttg tcgtggatgt caacgacacc
      541 aaacatctcg aaaagatcct caaaagcatc cgtcaggtgg atggcgtgct tgaggcgaag
      601 cgggtgagga agggatagcc ggagggcagc ggaaggatga tcagtcggcg gggattcttc
      661 gaaacggtga ttgcagttgt tgcaggggtg gtcggtctgg gcctctccat tccgctggcg
      721 ggctattcga tctggcccgc tctcaagaag agagagaccg gatgggttga tctcggtccg
      781 atcgagcaac ttaaatcggg tgagcccaag cagttagagt ttcttgcgac tcaaatggac
      841 gggtggctca agacctccat cacacaatgg gtctgggccg tccgccatag cgatgaagaa
      901 atcacagtct actctccgat ctgcacccat ttaggctgcg gataccattg ggattcgggt
      961 gaagggcaat ttgcctgtcc ctgccacggg agtatttttt ctattgacgg caaagtcctg
     1021 gccggtcctg ctccccggcc tcttgatacc ctccctgtca aaatcgaaaa tggccgcctt
     1081 tttatcactt acgaacagtt caagtctggg acgaaggaga aggtcgcaat ctgagggata
     1141 ccaggtctcg gtgctgaaaa ggatctacaa ttttctggat aaacgtcttc atctcacgtc
     1201 tcttgtccgg catgctctgg atgagccgat tccgggaggg gcatcctgga ttta
//
LOCUS       DATMKH010000365         1052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2737832, whole genome shotgun sequence.
ACCESSION   DATMKH010000365 DATMKH010000000
VERSION     DATMKH010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2737832"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="VNV63_04420"
     CDS             <1..264
                     /locus_tag="VNV63_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61899.1"
                     /translation="ENGHTVPAVETLEKFARALEIPMYQLFYEGEEPPKPPSLPTRKA
                     DSKLFGTSGKDARVLEKFRRLLSQTDAKDQGILLHMAKKMARR"
     gene            complement(472..747)
                     /locus_tag="VNV63_04425"
     CDS             complement(472..747)
                     /locus_tag="VNV63_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61900.1"
                     /translation="MPKISDEELKQKEAALSAEIRRLLPSFVESCRKNEDMVIIHQDS
                     FAADYQQDEYALLGMAIKFAGLHGKEIRIIGRNRETLKGSGKPELIQ"
     gene            857..>1052
                     /locus_tag="VNV63_04430"
     CDS             857..>1052
                     /locus_tag="VNV63_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61901.1"
                     /translation="MPVVYVIAPDWTMRTAVRAELREKGVEALGMDLADDVGRVIASG
                     GLPNVVVLEATSDLLGDPRIR"
BASE COUNT          232 a          267 c          287 g          266 t
ORIGIN      
        1 gaaaacggac atacggttcc ggctgtagaa acgctggaga agtttgcccg cgcccttgaa
       61 atcccgatgt accaactttt ttatgaaggc gaagagcctc ctaagccgcc gagcctgccg
      121 acgcggaaag ctgattccaa gctgttcgga acctccggta aagacgcgcg ggtgctggag
      181 aagtttcgta ggcttctcag tcagacggat gcgaaagacc aaggcatcct attgcatatg
      241 gcaaagaaga tggcgcggcg ttaggtcgtt taagattaat tgtctgaggc tgggcactcc
      301 gacatgcgtg gttcagtccg tcttcccata tcggtgggag gcgtgaatga cctgccggag
      361 cgccctaggg caaactttta acttgcgacc gcaagaatta caaggttgag atcttcgaaa
      421 aagacggaga ttccatcctt aacacttcca ctcgcaagct tggaaatccc atcattgaat
      481 aagctcaggt ttcccagatc ctttgagtgt ttccctgttc cttccgatga tgcggatttc
      541 tttcccgtgg agccctgcga actttattgc catgccgaga agcgcgtact catcttgttg
      601 gtagtctgcg gcgaatgaat cctgatggat gatcaccatg tcttcatttt tacggcagga
      661 ctcaacgaag ctgggcaata aacgcctgat ctccgccgag agagccgcct ctttttgttt
      721 gagttcttcg tctgatattt tgggcatttt tccttacctc ccgtcttctc aaggagattc
      781 agtcgacggt cctgcgcgga acaacagtat atcgttccac tggctgggtg cgaagaggta
      841 tgctcctctg aaaagtatgc cggttgttta tgtaatcgca ccagattgga caatgcgcac
      901 cgcggtccgc gctgaactgc gcgagaaggg cgttgaagcg ctcgggatgg atttggcgga
      961 cgacgtgggt cgcgtcatcg catcgggtgg gcttcccaat gtcgttgtcc ttgaagccac
     1021 gtcagacctt cttggcgacc ctagaatccg ga
//
LOCUS       DATMKH010000366         1045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1768110, whole genome shotgun sequence.
ACCESSION   DATMKH010000366 DATMKH010000000
VERSION     DATMKH010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1768110"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..>1045)
                     /locus_tag="VNV63_04435"
     CDS             complement(96..>1045)
                     /locus_tag="VNV63_04435"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HXC61902.1"
                     /translation="ASFDLIAGTSESTRAKTAETFLELLGARVRNPNTRSAYKVAWRS
                     FLAFCSARQLELESVKAYHVGAWLDQHPGSRSTQRQHLAAVRLLFDSLMMRGVVEYNP
                     AARARPPRLVRESSHTPVFEEAEIVAFLDSINLESLKDIRDKAIFSVLFYSWCRVSAL
                     INLTVADYYERGGTRWLRFQEKRGKEHEVPVHSKAKEAVDFWLNQSLLASNPSAPLFP
                     SFGKNRETIEQRRLDRRSVLKLVEKRAKASGILKRVCCHSFRATGVTEYMNSGGTIEI
                     AQRIAGHTSPATTRIYDRSGDVLTLEEIERVQIGKKQEI"
BASE COUNT          235 a          306 c          265 g          239 t
ORIGIN      
        1 cgaacactca cattcttcaa aaagcccaac tcttgaatcc gtgtgttgaa gattggaaag
       61 gggaaaatcc gtctagatgg ctcgaggcgc ggaacttaaa tttcttgttt cttcccgatc
      121 tgcactctct cgatctcttc aagggtcaac acgtccccgg atcgatcata aatccgggtg
      181 gtcgctggac tggtatgtcc ggcgatccgc tgcgcgatct cgatggtgcc ccccgaattc
      241 atgtattcgg tgacgcccgt cgcccggaaa ctgtgacagc agactctttt caggattccg
      301 ctggccttcg cccgtttctc aaccagcttc aagacactcc gacgatccaa gcgcctctgt
      361 tcaatcgttt cacgattctt gccgaaggaa ggaaaaagtg gagcactcgg attcgacgct
      421 agaagggact ggttgagcca aaaatcgacc gcctctttag ccttcgagtg caccggtacc
      481 tcgtgttctt tccctcgttt ttcttggaac cgtaaccagc gcgtgccgcc gcgttcgtag
      541 taatcggcaa ccgtcaggtt gatcagggca gacacccggc accaggaata gaagagaaca
      601 ctgaagatcg ccttatcccg aatgtctttt agcgattcca agtttatcga gtccaagaac
      661 gcaacgattt cggcctcttc aaagaccgga gtgtgggagg attctcgaac cagcctcggt
      721 ggcctcgccc gggcggctgg attatactca acgacccctc gcatcatcag cgaatcgaag
      781 agcaaccgga cggccgccag gtgttgcctc tgtgtcgacc tgcttcctgg atgctgatcc
      841 aaccacgctc ccacgtgata ggcctttaca ctctcaagct ccagctgacg ggccgaacaa
      901 aatgccaaaa atgagcgcca tgccacctta taagcgcttc gcgtattggg attgcgcacc
      961 cgcgctccca agagctcaag gaaggtctcc gcggttttgg ctcgggtcga ttctgaggtg
     1021 cccgcgatta gatcaaagga agcca
//
LOCUS       DATMKH010000367         1204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4005877, whole genome shotgun sequence.
ACCESSION   DATMKH010000367 DATMKH010000000
VERSION     DATMKH010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1204
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4005877"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1204)
                     /gene="aspS"
                     /locus_tag="VNV63_04440"
     CDS             complement(<1..>1204)
                     /gene="aspS"
                     /locus_tag="VNV63_04440"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HXC61903.1"
                     /translation="PENYPEVAKEAHSLHDEDVIRVKGKVAPRLEGTANPKLPTGEIE
                     IVVSELTILNQCAPLPFQLDANLSNEDLRLTYRYLDLRRPAMVRQLKARHILNRTTRN
                     YLDREGFLEIETPILSKSTPEGAREFLVPSRVMPSLFYALAQSPQQYKQLLMVAGAER
                     YFQIAKCFRDEDPRADRITELTQIDIEASFITQEDIFRLVEGLLKEIFIATREVTIPR
                     PFPTLTYQEAMDRFGSDKPDLRFGTELIDLSETFRNSQFKVFRAALDQKGVVKAINAK
                     GFANITAGQIEEVTNLAKQAGAKGLAFIKVENGEWKSPIVKFFSESERQSLIEKMGIE
                     EGDLILFGADSWETVCTVLGRLRLKVAEFTKLITDPDELNFLWVTDFPLMSFDPAENK
                     WNAMHHPFT"
BASE COUNT          267 a          309 c          307 g          321 t
ORIGIN      
        1 gtgaacggat gatgcatcgc attccattta ttctcggccg gatcaaacga catcaaaggg
       61 aagtcagtta cccagagaaa atttaactcg tccggatccg tgatcagctt ggtgaattca
      121 gctaccttca gtcgaagtcg ccccagcacg gtacagacgg tttcccaact atccgcgccg
      181 aaaaggataa gatcgccctc ttcgattccc attttctcga taagcgactg tcgttcggac
      241 tcggagaaaa acttcacgat gggcgacttc cattctccgt tttcgacctt gataaaggcc
      301 aatcctttgg cgccggcttg cttagccaga ttggttacct cttctatctg gccggctgta
      361 atgttggcga accctttcgc attgatcgcc ttcacaaccc ctttctgatc cagggccgct
      421 ctgaatactt tgaattgaga gtttcggaaa gtctcactga gatcgatcag ctctgtgcca
      481 aatcgaaggt cgggtttgtc gctgccgaaa cggtccatcg cttcctgata agttagcgtt
      541 gggaacggcc tgggaatggt tacctctcgg gtagcaatga aaatctcttt tagcagacct
      601 tctaccaggc ggaagatgtc ctcttgggta atgaacgaag cctcgatatc gatctgggta
      661 agctcggtga ttcggtcagc acgcgggtct tcatcgcgaa agcatttggc gatctggaaa
      721 tacctttctg caccggccac catcaacagc tgcttgtatt gctgaggcga ttgcgccagg
      781 gcatagaata ggctcggcat gacccggctt ggcaccaaga attctctggc gccttcgggt
      841 gtgcttttgg agagaatcgg agtttcaatt tccaagaaac cttctcgatc caaatagttt
      901 cttgttgtcc ggttgagaat gtgtctggcc tttagttggc gaaccatggc tggccgcctc
      961 agatcgagat agcggtaagt taaccggaga tcttcattgc tcaggttggc gtccagctgg
     1021 aacggcaatg gggcgcattg gttcaaaata gtcagttctg aaaccacgat ctcaatttcc
     1081 ccggtaggca atttcggatt ggctgttcct tccaggcgag gggcgacttt accctttacc
     1141 ctgataacat cttcgtcgtg gagcgagtgc gcttccttag caacctcggg ataattctca
     1201 ggcc
//
LOCUS       DATMKH010000368         1816 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1136510, whole genome shotgun sequence.
ACCESSION   DATMKH010000368 DATMKH010000000
VERSION     DATMKH010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1136510"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="VNV63_04445"
     CDS             complement(<1..575)
                     /locus_tag="VNV63_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011733986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HXC61904.1"
                     /translation="MAVRIYNTLTGKKGIFKPLRHGRIGMYVCGVTVYDRCHLGHARS
                     AVAFDVIRRYLKYKKYRIKYVRNFTDMDDKIIQRANREGKPWTEIVKRYLAAYEEDMK
                     RLGVEKPDREPKATDYIPDMIHLIRRLVKKGLAYSVNGDVYYQVEKFKSYGKLSKRKP
                     DELLAGARVEVDKRKKNPLDFALWKAAKPGEP"
     gene            complement(724..1356)
                     /locus_tag="VNV63_04450"
                     /pseudo
     CDS             complement(724..1356)
                     /locus_tag="VNV63_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511647.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
     gene            complement(1398..>1816)
                     /locus_tag="VNV63_04455"
     CDS             complement(1398..>1816)
                     /locus_tag="VNV63_04455"
                     /inference="COORDINATES: protein motif:HMM:NF014077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HXC61905.1"
                     /translation="FNDGEFNFAAAAEFAVRLARLIVEHGLPKDTLLNVNVPNLPISA
                     IRGVKVTCLGKRIYDRSNIVAKTDPRGRGYYWIGANETSWEERKDTDDLAIRDRMVSV
                     TPIHLDLTNYAVLERLRGWEPLLNKKARRRPKEKQV"
BASE COUNT          395 a          528 c          440 g          453 t
ORIGIN      
        1 ggttcccccg gcttggccgc tttccatagg gcaaagtcca atggattttt cttccgctta
       61 tccacttcca cacgggcacc ggccagaagc tcatccggct tacgtttcga aagcttgcca
      121 tagcttttaa acttctcgac ctggtaatag acatcgccat tcacggaata agctaaacct
      181 ttcttgacca gcctccggat cagatgaatc atgtcgggga tgtaatccgt cgccttaggc
      241 tctctgtccg gtttctcgac acccagccgc ttcatgtctt cctcgtatgc cgcgagatac
      301 cgctttacga tctcggtcca gggcttgccc tcccggttcg cccgttggat gatcttgtca
      361 tccatgtccg taaagttccg gacgtatttg atcctgtatt ttttatactt cagataccgg
      421 cggattacgt cgaaggcaac ggccgagcgg gcatggccga gatggcagcg atcatagact
      481 gtcacaccgc agacgtacat gccgatccga ccatgacgaa gcggcttaaa aattcccttt
      541 ttgcccgtga gggtgttgta gattctaacc gccattgtca gttgctagct tttcttgact
      601 ctatatcggg atggccgaga gatttaaccg aggacccatt gaccgatctt agacagaatg
      661 atctccatca tccgaaacgt gattcaaact gccacccaag ttgttgccag atccttcggg
      721 ttcccagccg aatgccccga ctagtggaac aaagacacaa cctgtcagat gggtagccgc
      781 cagaccttcc ggactcttca caatcttttt gagcatctgg ctggcacgat ctcctaccgg
      841 gatcacaagg cgtcctccgg gcttgagttg atccagcaga gagggcggga tgtccggggc
      901 cccggctgta acgatgatcg cgtcgaaagg ggactcctcc ggccaaccat atgttccatc
      961 aaacagtttg agggcgacat tgaaatattg aagccgattc agggaggttt gtgccttttg
     1021 aaataaggag cggacccttt cgactgagca tacctttgcg gcgagttcgg ccagaatcgc
     1081 ggtctggtaa cctgaaccgg tcccgatctc caagaccttc tccgtaccgg tcaagtccag
     1141 ggcctcggtc atccgggcca ccatgtaggg ctgtgagatt gtctgcttct caccgatggg
     1201 gagggcgtga tccccgtagg accgatccca gaacgcctct tccacaaaga gatggcgcgg
     1261 tacctttcgc atcgcttcaa ttactcgagg acctcgaatc ccgcggggga tcaactgatc
     1321 ctccaccatc cgatgccggg ccttctcgaa actcacgatt gattttccga atattctgct
     1381 tttgaacata aagcgtctca gacctgtttt tctttcggcc ttcgacgggc ttttttgtta
     1441 agcaatggct cccatcccct cagccgttcc agaaccgcat agttcgttaa atcgagatga
     1501 atcggcgtca cggagaccat cctgtcacga attgccaaat catcggtgtc tttgcgctcc
     1561 tcccatgacg tctcattggc cccgatccag tagtagcccc gtccccttgg atcggttttg
     1621 gcaacgatat tgctccgatc atagatcctc ttccctaaac aggtgacctt cacacctcga
     1681 atggccgaga tcggcagatt cggcacattc acgttgagca aggtatcttt gggaagaccg
     1741 tgctcgacga tcagacgtgc caaccggacc gcaaactctg ccgcagcggc aaaattaaat
     1801 tctccatcgt tgaaca
//
LOCUS       DATMKH010000369         1207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2740630, whole genome shotgun sequence.
ACCESSION   DATMKH010000369 DATMKH010000000
VERSION     DATMKH010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2740630"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1203)
                     /locus_tag="VNV63_04460"
     CDS             complement(<1..1203)
                     /locus_tag="VNV63_04460"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HXC61906.1"
                     /translation="MRAEGGVTFAQDPQTAKFDGMPRSAIESGCIDFVLSPEKIAAEL
                     SWMSKHPTLRRKSTDSHQEPKDDDHVFNSILNLVSTSQGADFSKYKPNTIHRRTQQRM
                     ASLGIASLVGYLTYLGGHPEEAAKLSNHVLIPVTEFFRNPEVFEDLTSTIYPAIVGEY
                     GKETIRIWVVACSSGEEVYSLAITLLEFLGAKADNLHIQIFGTDISEKVIAKARSGIY
                     PDSAMKNLSGDRIAKFFTKETGGYRVNKTVRDLCVFARQDVAKDPPFSNLDLISCRNF
                     LIYVQPELQEKIISTLHYALNPSGFLLLGSAESAASYPLMFTPLNKKNKIYSKKIGNH
                     RQQRTFKTTRTPIQASVSPPAKAQPIRRASQNEAGLQQVADRIVLKECAPPGVIVNDS
                     LEILQFRGR"
BASE COUNT          267 a          327 c          316 g          297 t
ORIGIN      
        1 tcgcccacgg aattgaagaa tttcgagaga atcgttgaca atcacccctg gcggcgcaca
       61 ctccttgagg acaatccgat ctgctacctg ttgcagtcca gcttcattct ggctcgcacg
      121 ccggatgggc tgcgccttcg ccggtggcga tacactcgcc tgaattgggg tacgggtcgt
      181 cttgaacgta cgctgctggc gatgattgcc aatctttttg gaatagatct tgttcttctt
      241 gttcagtggc gtgaacatca gcggataaga agctgcactc tcggcgcttc ccagcagcag
      301 aaagccggat ggattcaaag cgtagtgcag ggtcgagatg atcttttcct gcagctccgg
      361 ttgaacataa ataaggaaat tccggcacga gatcagatcc aggtttgaga aggggggatc
      421 cttggcgaca tcctgccgcg caaaaacaca tagatcgcgc accgtcttgt tgacccggta
      481 tcctcctgtc tccttcgtga aaaactttgc aatgcggtcc cctgagaggt tcttcattgc
      541 gctgtcgggg tagatgccgg agcgggcctt cgcgatcacc ttctcactaa tgtctgtccc
      601 aaagatctga atgtggaggt tatcggcctt tgcacccaga aattccagca acgtaatcgc
      661 cagcgaatac acctcttctc cgctggaaca ggcaactacc catatgcgga tggtttcttt
      721 cccgtattct ccaactatcg ccggatagat cgtacttgtc aggtcctcaa agacctccgg
      781 gttccggaag aactccgtta cgggaatcaa aacgtgattg gagagcttcg ccgcttcttc
      841 tggatgccct ccgagatagg tcaaataccc aacaagagaa gctatcccga gagatgccat
      901 gcgctgctgc gtacgtcgat gaatcgtatt gggcttgtat ttgctgaagt cggcgccttg
      961 cgaagtgctt accagattca aaattgaatt gaaaacgtgg tcatcgtcct tcggctcttg
     1021 gtgcgaatcc gtcgacttcc tccgaagggt gggatgcttg ctcatccacg acaactctgc
     1081 tgcgatcttc tccggagata aaacgaaatc gatgcagccg gactcaatgg cgctccgagg
     1141 cataccgtcg aacttggctg tctgtgggtc ttgggcgaaa gtcaccccac cttcggcgcg
     1201 aatcgct
//
LOCUS       DATMKH010000370         1808 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4650481, whole genome shotgun sequence.
ACCESSION   DATMKH010000370 DATMKH010000000
VERSION     DATMKH010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4650481"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1745
                     /locus_tag="VNV63_04465"
     CDS             <1..1745
                     /locus_tag="VNV63_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HXC61907.1"
                     /translation="ELDQRKIEREIKKQERELKKKGVSIPEEPAVPRPEPKVFDTVAA
                     PSPPKKPSRPAPPPKPAEEVYELPSVGLFDPVEAESRKASDPAELKEIQAVLVDTLKQ
                     FNIDVAPGDITRGPTITRYEVYPARGVRVDRITSLERDLARATRAERINILAPIPGKD
                     TVGIEIANTKKVKVALREIVESPDWAQTKMKIPIVLGKDVYGKIILGDLAQMPHCLVA
                     GTTGSGKSVCINAVIASILLRFSPADLQFVMIDPKVVEMQMYNSLPHLKIPVVTDPKK
                     VLLALRWVVSEMEKRYRIFAHVGVRNVSGFNARPKPKTQKELDLEAESGRDGQTRNVP
                     DGTRNARDEDLIIPEKLPYIVVIIDELADLMQTAPADVESAIARITQMARAAGIHMIV
                     ATQTPRADIITGVIKANIPTRIAFQVASSLDSRVILDENGAERLLGQGDMFYRPPGTS
                     RLVRSQGVLVTDDEIHRLVSFVSSQAQPSFDADVQNQLESSSSPEEDVTEEDEELVSR
                     CLEIIRQEKKASTSMLQRRLRLGYTRAARVVDILERRGILGPGEGAKPRQILVDLDAL
                     VGESSMDPGHLFGG"
BASE COUNT          451 a          458 c          511 g          388 t
ORIGIN      
        1 tggaattaga tcagaggaag atcgagaggg agatcaaaaa acaggagcgc gagcttaaaa
       61 agaaaggcgt ttcgataccg gaagagccag cggttccacg ccctgaaccg aaagtgttcg
      121 acaccgtcgc ggcaccctcc ccgccgaaaa aaccaagtcg tccagctccg ccgcccaaac
      181 ctgcagagga ggtctacgag ctacccagcg tcggtctttt cgacccggtt gaggctgaaa
      241 gccgaaaagc gagcgatccc gcggaactga aggagatcca ggcggtcctg gtcgataccc
      301 tcaaacagtt caatattgat gtcgccccgg gagacatcac tcgaggcccg acgatcaccc
      361 gctacgaggt ctatccggcc cggggggtgc gcgtggatag aataacgagt ttggaacgcg
      421 acctggccag agcaactcgg gctgaacgga ttaacattct tgctcctatt cccggcaaag
      481 acacggtggg gatcgagatt gctaatacga agaaagtcaa agtagctctg cgagaaatcg
      541 tggagtcacc ggattgggcg cagaccaaaa tgaagattcc tattgttctg ggcaaggatg
      601 tgtacgggaa gatcattttg ggtgatctcg cgcagatgcc gcactgtctg gtggcgggaa
      661 cgacgggtag cgggaagagc gtttgtatca atgcggttat agccagcatc ttgctccgct
      721 tttccccggc tgatcttcaa ttcgtgatga tcgacccaaa ggtagtggag atgcagatgt
      781 acaactcgct gcctcactta aagatccccg tcgtcaccga cccgaagaaa gttctcttgg
      841 cgttacgttg ggttgtttcc gagatggaga agcgataccg gattttcgcg cacgtcgggg
      901 tgcggaatgt ttccgggttc aacgccagac cgaaacccaa aactcagaag gaactggatt
      961 tggaagctga atccggtcga gatggtcaaa cgcggaatgt tcccgatggt acccggaatg
     1021 cccgcgacga agacttgatc attcccgaga aactgcccta catcgtcgtc atcattgatg
     1081 aattagccga tctcatgcaa acagcgcccg ccgatgtaga gagcgccatt gccagaatta
     1141 cccaaatggc ccgtgcagcc gggatccaca tgatcgtggc gacccagaca cctcgagcgg
     1201 acattatcac cggcgttatt aaggcgaaca tcccgacacg gatcgcattt caagtcgctt
     1261 ctagtttaga tagtcgggtt attctcgacg aaaacggggc ggaacggcta cttggacagg
     1321 gagacatgtt ttatcggcct ccagggacct cgcggttggt tcgttctcag ggagtgttgg
     1381 taactgatga cgagattcat cggttggtta gtttcgtctc gtctcaggct caaccgtcgt
     1441 tcgacgcgga tgttcaaaac cagttagaat caagctcctc acctgaagag gatgttaccg
     1501 aggaggatga agaactagtc agtcgttgct tagaaattat ccgccaagaa aaaaaagctt
     1561 ctacctctat gctacagcga cgtctacgcc tcggatatac ccgggcggcg agggtagttg
     1621 acattcttga acgcagaggc attttgggcc ctggcgaagg agctaaacca agacaaatcc
     1681 tggttgatct cgacgctctg gtcggcgaat ccagcatgga tcccggccat ctcttcgggg
     1741 gctagtactt cgttctatcg gtcaaatcgt ttttcggatg gttgagacag ttggcagaag
     1801 gcttcaga
//
LOCUS       DATMKH010000371         1076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3057316, whole genome shotgun sequence.
ACCESSION   DATMKH010000371 DATMKH010000000
VERSION     DATMKH010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3057316"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="VNV63_04470"
     CDS             <1..608
                     /locus_tag="VNV63_04470"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HXC61908.1"
                     /translation="GKIALLNLASPQGRIEDILGLFVKAPHSPMSGEVALKVKVEIPS
                     GKQPFLDRVKMEGTFGVDDGSFSSPQTQKNVNELSAGARGEKMEDAETVLTDLKGQVG
                     LESGIAKFSDLSFGIPGAKARANGTYNLVNEKVDLHGRIRVDSKISKTETGMKSFLLK
                     ALDPFFKKKKKGEVVPVHITGTYEHPQFGLDMTKQKEHARH"
     gene            complement(708..>1076)
                     /locus_tag="VNV63_04475"
     CDS             complement(708..>1076)
                     /locus_tag="VNV63_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61909.1"
                     /translation="LLGLVFELGGHVQGYHQNRNVWRLSSDFFRGIQTIHFRHLEIEH
                     DYIGGSLLGSFDGFSTVGRLTAHPPGVLLFQESPQAAAHKSAVVYQKDSDGRGLRPGV
                     QERHTKPYNRGQRASIHRIA"
BASE COUNT          283 a          292 c          294 g          207 t
ORIGIN      
        1 aagggaagat tgcactcctc aatctggcct ccccgcaggg acgaatcgaa gacatcctgg
       61 gtctgtttgt caaggctcct cactccccga tgtcaggaga ggtcgcgctc aaagtcaagg
      121 tggagattcc gtccgggaag caaccgtttc tcgacagagt aaagatggag ggcactttcg
      181 gtgtcgacga tggcagtttc tcgagtccgc aaacgcagaa aaacgtgaac gaactgagtg
      241 ctggagccag aggcgaaaaa atggaagatg ccgaaaccgt gctcaccgac ctgaaggggc
      301 aagtcgggct cgagagcgga atcgcgaaat tctctgacct ttcattcggc atacccgggg
      361 ccaaggcgcg ggcaaatggc acgtacaacc tggtgaacga gaaggtcgat ttgcatggca
      421 gaatccgggt tgacagcaag atttcgaaga ctgaaaccgg tatgaagtcg tttcttctca
      481 aggccctgga tccctttttc aagaaaaaga aaaagggcga agtcgtcccc gtccatatta
      541 cgggcacgta tgagcatccc cagtttggac tcgatatgac aaaacaaaag gaacatgcac
      601 gccattagag acgcgcacgc attttgcccc tcccctttct cccagtgctc ccggactacc
      661 ccgaaaccgt taggaaaagg gatgaggggg caacaatcgg cgccagccta cgcgatgcgg
      721 tgtatagacg cccgttgtcc ccgattgtac ggtttagtat gtctttcctg gacgccaggc
      781 cggagacctc gcccgtccga atccttctgg tagacgacag cagacttatg cgccgctgcc
      841 tgcggactct cttggaacaa caaaactcct ggcgggtgtg cagtgaggcg accaacggtc
      901 gagaagccat cgaaagagcc cagcaaactg cccccgatat aatcgtgctc gatttccaga
      961 tgcctgaaat gaatggtttg gatgccgcga aagaaatcag aagacagtcg ccagacattc
     1021 cgattctgat ggtaaccctg cacatgtccc cccaactcga agaccaagcc aagaag
//
LOCUS       DATMKH010000372         5278 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4007618, whole genome shotgun sequence.
ACCESSION   DATMKH010000372 DATMKH010000000
VERSION     DATMKH010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 5278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5278
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4007618"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            60..1817
                     /locus_tag="VNV63_04480"
     CDS             60..1817
                     /locus_tag="VNV63_04480"
                     /inference="COORDINATES: protein motif:HMM:NF004952.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HXC61910.1"
                     /translation="MKEKEKMKGGSLEKLQALPQAEGEAMDMNMLYEETFKNLEEGTV
                     VEGTVVDIQTDGVLVDIGYKSEGVIPREEFTPAELSQIKVGDRIQIYLDEREDSEGKP
                     VLSKEKAEKMKVWEDLEKSSKKGEVVEGKILSRIKGGMIVDIGVKAFLPGSQIDLRPI
                     RDLDSLVGKSYPMKIIKMNQRRGNIVVSRRVLLEESRDRKRQTTLAALEEGQRVHGVV
                     KNITEYGAFIDLGGIDGLLHITDMSWGRISHPSELFSVGSKVEVTILKYDRETNRVSL
                     GYKQTTADPWSEIEKKHPVGSKVQGKVVSLTDYGAFVELEKGIEGLVHISEMTWTHEI
                     RHPSKIVSVGQTVETLVLHVDKDNRKISLGMKQIEPNPWDLVEKTYPVGSKIQGKIKS
                     LTDFGAFVGLEEGIDGLIHVSDMSWTKRLTHPSELFKKGQPVEAVVLKIDREKERLSL
                     GYKQLTPDPWEKDIPEKYPVGSDVQARVVKITDFGIFVEMEDGVEGLIHVSEAGLEAG
                     TKIDKGAKLNDQLMARVIKMDPAERKIALSIKEYKRSTDQKDVEEFHRSQGPVDQSLG
                     KVAQRSAKKKESHPESDKQ"
     gene            1826..2710
                     /gene="sppA"
                     /locus_tag="VNV63_04485"
     CDS             1826..2710
                     /gene="sppA"
                     /locus_tag="VNV63_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HXC61911.1"
                     /translation="MKNRPLLIGLLVFGVLGLVFFLWVYRTTILTESPVLTDDRIALI
                     RIEGVILDSKDTIDELKRYRENPGIKAILLRIDSPGGAVVPSQEIYEEVKKAREGGKK
                     KVVSSMGTLAASGGYYIASATDKIVANPGTLTGSIGVIMELANVEGLMKKVGVESIVI
                     KSGAHKDLVSPFRKMREEERQILQNVLDDVHEQFIEAVSEGRGIPIEKVRSLADGRIF
                     TGRQAKGIGLVDELGDLQDAIRITASLVGIKGEPKVVETKKRFSFFDLFRDQFGAKWG
                     YFPLAPGGVQLKYLLTFG"
     gene            2722..3000
                     /locus_tag="VNV63_04490"
     CDS             2722..3000
                     /locus_tag="VNV63_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013886054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HXC61912.1"
                     /translation="MTKAQLIEKVSEQLKVMTKRQTEIIVNTVFNSIKEALAKGDKIE
                     IRGFGSFRLRSRKMRDGRNPKTGASVQVPAKRVPFFKAGKELKQLVDN"
     gene            complement(3006..4283)
                     /gene="hisS"
                     /locus_tag="VNV63_04495"
     CDS             complement(3006..4283)
                     /gene="hisS"
                     /locus_tag="VNV63_04495"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006003214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HXC61913.1"
                     /translation="MGKTGYIVAMKISAIRGVKDILPDEIVRVQQLEDTARRLFERFG
                     YSEIRIPIFEVTELFTRGIGTTTDIVEKEMYTFEDRDGKKLTLRPEGTASVVRAYIEH
                     QMYLQKPVTKLYYLGPMFRHERPQAGRFRQFYQIGIEALGTSSAAMDVEILAVLIWLL
                     QEADVTGIQLELNSLGDSVCRPQYRKALKSFLKDNLHKLCEECQKRYETNPLRILDCK
                     NESCRAVTAAAPSPLDHLCQDCLAHFEQVKSGLDQLKIPFVINQRLVRGLDYYNRTAF
                     EVTTGRLGAQNSIAGGGRYDGLTEELGGPPTPGIGFAMGVERIASLMKPVEPPKPDFF
                     FALLGEEALKQMLPVIMQLRREGLRIEIDYGGGNLKKQMGLSDKLGARYTLILGENEL
                     KSGKAILRNMTTKEQQELVLSTLSQTLKSLVKP"
     gene            4652..>5278
                     /locus_tag="VNV63_04500"
     CDS             4652..>5278
                     /locus_tag="VNV63_04500"
                     /inference="COORDINATES: protein motif:HMM:NF028174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase dimerization domain-containing
                     protein"
                     /protein_id="HXC61914.1"
                     /translation="MRIGGIPESFLEHFLNSAHLLPSPLIETLGAMALCRTVMAGNKL
                     GVFELLAQEPQSAEAISKKLSCQRPGMEALLEALAASGYLERRGSLYANSPMARRWLT
                     ADSPYSLGHYLELDYLQWSWWGRLEEMVQTGKAVDLHQSLKGNPEAWKVYLLGLKDLA
                     GWTSREILRRVRRPRGAKYLLDLGGGHGGYSEAVCRRYTEMRAVVFDLP"
BASE COUNT         1315 a         1251 c         1547 g         1165 t
ORIGIN      
        1 gggaatcaag agacagatga ggcgctggcc tcgtccaggg aaggcgtggg gcggagactg
       61 tgaaagagaa ggaaaaaatg aaaggaggat ctttggagaa gctgcaggcg ctgcctcagg
      121 cagaaggtga agcaatggac atgaacatgc tctacgagga gacctttaag aatctggaag
      181 agggaaccgt ggtggaggga accgtggtgg atatccagac ggacggggtg ctggtggaca
      241 tcgggtacaa atcggaaggt gtgatccccc gcgaagagtt cactccagcg gaactttccc
      301 agattaaagt gggagacagg attcagatct atctcgatga gcgtgaggat agcgagggaa
      361 agccggttct ctccaaggaa aaggcggaga agatgaaggt ctgggaggat ctggaaaaat
      421 cttctaagaa gggcgaagtg gtggagggaa aaatcctgtc gcggatcaaa ggtggaatga
      481 ttgtggatat cggggtgaag gccttcttgc ccggctctca gatagatctt cgacctatac
      541 gggatttgga ttcgctggtt ggcaagagtt atcctatgaa gatcatcaag atgaaccaac
      601 gacggggcaa tatcgtggtc tcccgccgtg tcctcctgga ggagtccaga gaccgcaagc
      661 gacagaccac gctcgccgct ttggaggagg gacaacgcgt tcatggggtg gtaaagaaca
      721 tcaccgaata tggggccttt attgacctgg gcgggattga tggtcttctc cacatcacgg
      781 atatgtcgtg ggggcgtatc agtcatcctt ccgaactctt ctcagtgggt agtaaggtgg
      841 aggtcacgat ccttaaatac gatcgggaga ccaaccgggt atcgctggga tataagcaaa
      901 caacagcgga tccctggagc gagatcgaaa agaaacatcc ggttggttcg aaggtccagg
      961 ggaaagtggt cagcctgacc gattatggtg cctttgtgga acttgagaag ggaatcgaag
     1021 ggctggttca tatctcggaa atgacctgga cccatgagat ccgtcatccc tcgaaaattg
     1081 tctcggtcgg gcaaactgtg gagaccctcg tgctccacgt tgataaggat aaccgcaaga
     1141 tctccttggg catgaagcag attgaaccca acccctggga tctcgtcgag aagacgtacc
     1201 ccgtgggaag caagatccaa ggcaagatca agagcctgac cgactttggc gcctttgtgg
     1261 gcctggaaga ggggattgac ggcctgatcc atgtttcaga tatgtcatgg acgaagcgtc
     1321 tcacccatcc ctcggaactg ttcaagaaag gtcagccggt cgaggcggtc gtgctcaaaa
     1381 ttgatcgtga aaaagagaga ctctctctag gctacaaaca gttgacgcca gatccatggg
     1441 agaaggatat ccccgaaaaa tacccggtag ggagcgatgt ccaggcccgt gtggttaaga
     1501 tcaccgattt cgggatcttt gtggagatgg aagacggggt ggagggactg atccatgtca
     1561 gcgaggccgg tctggaagcc ggtaccaaga tcgataaggg agccaaactc aatgatcaac
     1621 tcatggccag ggtgatcaag atggatcccg cagaacgtaa aattgctttg agcatcaagg
     1681 aatacaaacg ttccaccgat cagaaggatg tcgaagagtt tcatcgcagt caaggtcccg
     1741 tagatcagag cctgggaaaa gttgctcaac gctccgccaa gaagaaagaa tcccatccgg
     1801 aatcagataa acaataaggt cattcgtgaa aaataggcca ttactgatag ggttactcgt
     1861 tttcggggtg ctagggctcg ttttcttttt gtgggtttac cgcacgacca tcttgacgga
     1921 gtctccggtg ctcacggatg accggatcgc gctgatccgg atcgaaggcg tgatcctgga
     1981 ctccaaagac acgattgatg agttgaaacg ataccgggag aatcctggga tcaaggcgat
     2041 cctcctgcgg attgacagcc ccggcggggc ggtggttcct tcgcaggaga tctacgagga
     2101 ggtcaagaag gcccgtgagg gaggaaaaaa gaaagtcgtg tcctcaatgg gcaccttggc
     2161 cgcttcggga ggttactata tcgccagcgc caccgacaaa attgtggcca accccggtac
     2221 ccttacagga agcatcgggg tgattatgga actggccaat gtcgaaggct tgatgaagaa
     2281 ggtcggcgtg gaaagcatcg tgatcaaaag cggagcgcac aaggacttgg tgtcgccgtt
     2341 ccggaagatg cgggaagagg aacggcagat tcttcaaaac gtcttagacg acgtgcatga
     2401 gcaatttatt gaagcggtca gtgaagggag aggaataccg atcgagaagg ttcgttcact
     2461 tgcggatgga cgcatcttca cggggagaca ggccaagggg atcggtttag tggacgaact
     2521 gggggatctc caggatgcga tccggatcac ggcttcactg gttggaatca agggggagcc
     2581 gaaggtggtc gaaaccaaaa agcgattcag cttcttcgat ctctttaggg atcagttcgg
     2641 cgcgaagtgg ggatatttcc cgctggctcc gggaggggtt cagttaaagt atctgcttac
     2701 attcggataa gaggagggcc tatgaccaag gcgcagctca ttgaaaaggt atcggaacag
     2761 ttgaaggtca tgacaaaacg tcagaccgaa atcattgtca atacggtatt taacagcatc
     2821 aaagaggctc tggccaaggg cgacaagatt gagatccgcg ggtttggcag ttttcgcctc
     2881 cgaagccgaa agatgcgtga tggaaggaac ccgaagaccg gggcttccgt gcaggtcccg
     2941 gcaaaacggg tccctttttt taaagcaggg aaagagctca agcaactggt ggataattag
     3001 ttgtcttaag gtttcacgag agactttaag gtttgggaga gggtagataa cacaagttcc
     3061 tgctgttctt tcgtagtcat gttccgaagg atcgccttcc cggacttcaa ttcattttct
     3121 ccaaggatca acgtgtatct cgccccgagc ttgtccgaaa ggcccatctg tttttttaag
     3181 tttcctccgc cgtagtctat ttcaatacgc agaccttctc ttcgaagctg catgatcacc
     3241 ggcagcatct gttttaaggc ttcctcgccc agaagagcaa agaaaaaatc cggtttcgga
     3301 ggttcgaccg gtttcatgag cgatgcgata cgctcgaccc ccatcgcgaa tccaatgcct
     3361 ggtgtaggcg gtccgcccaa ttcctccgtc aagccgtcgt atcggccgcc gccggcaatg
     3421 gaattttgtg cccccagcct tcccgtggtt acctcaaagg cggtccggtt ataatagtcc
     3481 agtccccgta caagccgctg attgattaca aaggggatct tgagttgatc cagaccgctt
     3541 ttgacctgtt cgaaatgagc cagacaatcc tggcagagat ggtccagggg agatggagcg
     3601 gccgccgtga cggcgcgaca gctttcattc ttgcagtcga gaatccttaa cggattggtt
     3661 tcgtaccgct tctggcattc ctcgcagagc ttgtgcaggt tgtccttcaa gaaagatttg
     3721 agcgcttttc ggtactgggg acggcagacc gaatccccca gactgttcaa ctccagttgt
     3781 atcccggtca cgtccgcctc ctggagcagc cagatcaaca ccgcaaggat ttctacatcc
     3841 atcgcggcac tggaagtccc gagcgcctct atcccgatct ggtagaattg ccggaagcgt
     3901 ccggcctgcg gccgctcatg ccggaacatg ggacccagat agtaaagctt cgtgaccggc
     3961 ttttgaagat acatctggtg ctcgatataa gccctcacca ccgaggcggt accttccggc
     4021 ctgagagtaa gctttttccc atcgcggtct tcgaaggtgt acatctcctt ctcgacgata
     4081 tcggtggtgg tcccgatccc gcgggtaaaa agctctgtaa cttcgaaaat ggggatccgg
     4141 atctcggagt aaccgaatcg ctcgaatagc cggcgggcgg tgtcctcaag ctgctggacc
     4201 cgaacgatct cgtccggaag aatatcttta acgccgcgga tggcagagat cttcatcgcc
     4261 acaatatagc ctgtctttcc catgaaggtc aatagcattt cagggattga cttgatttat
     4321 cttaagaatt gtagtaataa ttctaaaagg cagtggttct tcgttttgca aaaaaagagg
     4381 agatcattct ctggaagata tccaaggcca ggtcaagtgg accaacaagt tttcggacac
     4441 ccacgtgtcg gggttggaat ttgctgaggt gatcgatgag aataactatc cagccctcct
     4501 cccctatctg aaacaagttg agaatctctt tgtacagagt tttaatagct taattccctc
     4561 ctacagaagc acggaagacc gagtgaaaag ccggatgaag aacaacgttg accgggcgat
     4621 cggacaatcg ctgggggcct gaaagaccac gatgcgaatt ggaggaattc cagaaagttt
     4681 tctggagcat tttttaaact cggctcacct tcttccctcc cccctcatcg agacgctggg
     4741 ggcgatggcg ctctgccgga cggtcatggc cggaaacaag ctgggggtct ttgaactctt
     4801 ggcacaagag ccgcagtcag ccgaagcgat ttcgaaaaaa ctttcttgtc agcgtccggg
     4861 gatggaggca ttgctcgagg ctttggccgc ttccggctat cttgagcgcc gaggttcact
     4921 ttacgccaac agtccgatgg ccaggcgatg gttgaccgct gactcaccat actcgctggg
     4981 ccattatctc gaacttgact atctccagtg gtcctggtgg gggcgtttgg aagagatggt
     5041 tcaaacgggc aaggcggtgg atcttcatca atcactcaag gggaatccgg aagcctggaa
     5101 ggtctatctc ctgggtctga aagacctcgc aggctggacc tcccgggaaa ttttgagacg
     5161 ggtccgccgg cctcgcggcg ccaaatatct tctggatctc ggcggcggac acggaggata
     5221 ttccgaggcg gtctgccggc gatatacgga gatgagagcg gttgtgtttg atctgccg
//
LOCUS       DATMKH010000373         3442 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2741221, whole genome shotgun sequence.
ACCESSION   DATMKH010000373 DATMKH010000000
VERSION     DATMKH010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2741221"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="VNV63_04505"
     CDS             complement(<1..377)
                     /locus_tag="VNV63_04505"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HXC61915.1"
                     /translation="MPTFRYKARDKFGAAFNGTIETTTKDSVAAQLNTLGYVPVKIEE
                     ESRKGLLGPDFLERFQRISSEDLIIFSRQLATLISAGLSFTTSLDALIEQTENKRLKR
                     VITQVRRDLEGGSSFSDALARHPA"
     gene            complement(381..2081)
                     /locus_tag="VNV63_04510"
     CDS             complement(381..2081)
                     /locus_tag="VNV63_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HXC61916.1"
                     /translation="MPVPQQKRKMLGEMLVAENLLSHDQLERALAEQRQHGGRVGTIL
                     KSLGFVTEEDIIKALGKQMGIQHMVLTSVIIDPEMIKIIPEILARRHQAIPLFKKDKV
                     LTLAMADPLNVFAIDDIRHATGMEVQPVVSTEGDVLKAIDHYYSGTSMMREAVREADV
                     QGFGTPEVGQVIDLQRMADDTPVIKLVNTMIAQAVREGASDIHVEPDAEVLRIRYRVD
                     GLLHEVMTPPRNLQAGVTSRIKIMADLDIAEKRVPQDGRIQMKVAEKDIDIRLSTLPT
                     IFGEKIVMRLLDKGNLLLGVDQLGFSPEALKKFKKMITRPYGLLLVTGPTGSVKTTTL
                     YSVLKSINSFEKNVITIEDPVEYQVKMVNQVQVNPKAGITFANGLRSILRQDPDIIMV
                     GEIRDRETATIAIQAALTGHLVFSTLHTNDAAGAITRLVDIGVEPFLVASSIIGIVGE
                     RLVRKICVHCKQSYSPSPDLLKSLGLGDRKEITFMRGAGCQNCRGTGYSGRIGLFELM
                     AMDETIRNLVVAKTSTSGIRIAAQKAGHVGLREEGLLKALQGITALEEVLRITQEIET
                     "
     gene            complement(2096..3019)
                     /locus_tag="VNV63_04515"
     CDS             complement(2096..3019)
                     /locus_tag="VNV63_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HXC61917.1"
                     /translation="MRVLVTGGAGFIGSHLVDRLIQEGHEVIVVDNLSTGKKKNVHKD
                     AKFYKYDILNPKIERIFKREKPELVSHHAAQMDLRRSVVDPIFDAQVNILGLLNILEN
                     TVRYGTRRIIFASSGGAIYGEPRSFPVAEVHPTYPLSPYGVSKLAGEQYLHYYKEACG
                     LEYTSLRYGNVYGPRQDPFGEAGVVAIFAQKMLMGQKPVINGNGKQTRDYIYVGDVVE
                     ANMAAMHSGHNDIFNVASCKETSVNELFRYLTEITGSKAKEVYGPEKRGEQARCVLDC
                     SKIRRLLDWEPKVSLLEGLERTAAYFRKGPK"
     gene            complement(3016..>3442)
                     /locus_tag="VNV63_04520"
     CDS             complement(3016..>3442)
                     /locus_tag="VNV63_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61918.1"
                     /translation="QRDYTYIEDAVDGIMAALAYPAGAPSKSTGSQGKNTPSSGQNTG
                     PSGQNTGSSGRYTWNEMVNIGEAQTIELKELILLLEEGLGKKAVVEYLPMQPGDVPAT
                     CADLRKAKQLLGYRPRTGIKEGIQHFIEWYQQEGREMRL"
BASE COUNT          761 a         1013 c          805 g          863 t
ORIGIN      
        1 gccggatgtc tggccagagc atccgagaag gagctgcctc cttccagatc acgacggacc
       61 tgagtaatca cccgcttgag ccgcttgttt tcggtttgtt cgattagggc atcgaggctt
      121 gtggtgaacg ataacccggc gctaatcaag gtcgccaatt gccggctaaa gatgatcagg
      181 tcttcggaac tgatacgctg aaagcgttcc aggaaatcag gaccgagcag acctttcctg
      241 ctctcctctt cgatctttac cgggacatag cccagagtgt tcaactgagc cgctactgaa
      301 tccttggttg tggtctcaat ggtcccgttg aacgcggccc caaacttgtc tctcgcttta
      361 taacgaaagg tcggcatggc tcaggtctcg atctcctgtg ttatccgtag cacttcctcc
      421 agagcggtga tcccctgcaa ggcctttaaa aggccctctt cacgaagccc gacatggccc
      481 gctttctggg ccgcaatccg aattccgctc gtggaggttt tcgccaccac caagtttcta
      541 atcgtctcat ccatggccat cagctcaaac aggccgatcc ggccggagta gccggttcct
      601 cgacagttct gacagcctgc tcccctcata aaagtgatct ctttccgatc tcccaggccc
      661 aaactcttga gcagatcggg actaggggaa taactctgtt tacagtgcac acagattttc
      721 ctcacgagtc gctcgcctac aataccaatg atcgaagagg ctaccaaaaa gggttccacc
      781 ccaatatcca ccaagcgggt aatggcaccg gccgcatcat tcgtgtgcag cgtggaaaag
      841 accagatgcc cggtcaaggc agcctggatg gcgattgtcg cggtctcccg atcccggatc
      901 tcgcccacca taatgatgtc cggatcctgt cgaaggatgg accggaggcc gttggcgaaa
      961 gtgatcccgg cctttggatt gacctggacc tgattgacca tcttgacctg atactcgacc
     1021 ggatcttcga tcgtgatgac gttcttttca aatgagttta tacttttcaa cacggaataa
     1081 agcgtggtag tcttcacgct tccggtggga ccggtcacga gaagcaggcc ataaggccgg
     1141 gtgatcatct tcttaaactt tttaagcgcc tccggtgaga accctaactg atcgacaccc
     1201 agcagcaggt tccccttgtc caacaaccgc atcacgatct tttcaccaaa tattgtgggc
     1261 agagtggaaa gacgaatatc aatatccttt tcggctactt tcatctgaat ccggccatcc
     1321 tgcggaaccc gcttctcagc gatatccaga tcggccataa tcttgatccg ggaagtcacg
     1381 cctgcttgaa gattgcgggg gggagtcatc acctcgtgaa gcagaccgtc cactcggtat
     1441 cgtatcctca gcacctccgc atccggctca acatgaatat cactggctcc ctctcgcacc
     1501 gcctgagcga tcatggtatt gaccagcttg atcaccggtg tgtcatccgc catgcgttgc
     1561 agatcaatga cctgtcccac ttcaggcgtc ccgaaaccct gaacatccgc ctcgcggacc
     1621 gcctcacgca tcatcgaggt gccgctgtag tagtggtcga tcgccttcaa tacatccccc
     1681 tccgtgctga ccacgggttg gacttccatt ccagtggcat ggcgtatgtc atcaatcgca
     1741 aagacattga gcggatctgc catggccagt gtcaggacct tgtctttttt gaagagggga
     1801 atggcttgat gtcgtcgggc gaggatctcc gggatgatct tgatcatttc cggatcgatg
     1861 atcacgctgg tgaggaccat gtgctgaatt cccatctgct tgccaagggc cttgatgata
     1921 tcctcttccg tgacaaaacc gagactcttt aggatcgtcc cgacccggcc gccgtgttgt
     1981 ctctgctcgg ccagggcccg ctcgagttga tcgtgcgaca gaaggttttc cgcgacgagc
     2041 atctcaccga gcatcttccg cttctgttgg ggaaccggca ttaccgtcgc ctaccttact
     2101 tgggcccttt tctgaaatag gccgcagtcc gttctaaacc ctccagcaat gaaaccttgg
     2161 gctcccaatc caagagcctc cggatcttac tgcaatcgag gacacaacgg gcttgctctc
     2221 cccgtttctc cggcccatac acttccttgg ccttcgaacc cgtgatctct gtcaagtaac
     2281 gaaagagttc attcaccgag gtctccttac agctcgcgac attgaagatg tcattgtgtc
     2341 cactgtgcat cgcggccatg ttggcctcaa caacgtcacc gacatagata taatcccgtg
     2401 tctgttttcc atttccgttg atgacaggct tttggcccat caacatcttt tgtgcgaaga
     2461 tagccaccac cccggcctcg ccgaaaggat cttgccgtgg cccatagaca ttgccataac
     2521 gaagactggt gtactcgagt ccacaggcct ctttgtaata atggagatat tgttcaccgg
     2581 ccagtttgct aaccccatac ggcgaaagag gataggtcgg atgaacttcc gccaccggaa
     2641 aggatcttgg ctcaccatag atcgcacccc cggacgaggc aaagatgatc cgtcgagtgc
     2701 cataccggac tgtattctct aaaatattca ataatcccag tatgttcacc tgagcatcaa
     2761 aaatcggatc taccaccgat ctccttaagt ccatctgggc tgcgtggtga ctgaccagtt
     2821 ccggtttctc gcgcttaaaa attctttcga tcttaggatt aaggatatca tatttataga
     2881 atttcgcatc tttatggaca ttttttttct ttccagtcga gagattgtcc actacaatca
     2941 cctcatggcc ttcttgaatt aggcgatcca cgagatgaga tccgataaac ccagcacccc
     3001 ctgtcactaa cactctcata accgcatctc ccttccctct tgttgatacc attcgataaa
     3061 atgttgaatt ccttctttga tcccggtcct aggacggtaa ccgagaagct gtttggcctt
     3121 gcggagatca gcgcatgtgg ccggcacatc acccggttgc atcggcagat attctaccac
     3181 cgctttcttc cccagaccct cctccaaaag taatattaac tcttttaatt caatcgtctg
     3241 cgcctcacca atattcacca tctcattcca tgtgtatctc ccggaggagc ccgtgttctg
     3301 cccagagggg cccgtattct gcccggatga aggcgtattc ttcccctgag agcccgtgga
     3361 cttggacgga gcccctgcgg gatatgccag ggcggccatg atcccatcca ccgcatcctc
     3421 aatatatgta tagtcccttt ga
//
LOCUS       DATMKH010000374         1411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2094967, whole genome shotgun sequence.
ACCESSION   DATMKH010000374 DATMKH010000000
VERSION     DATMKH010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1411
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2094967"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(178..>1411)
                     /locus_tag="VNV63_04525"
     CDS             complement(178..>1411)
                     /locus_tag="VNV63_04525"
                     /inference="COORDINATES: protein motif:HMM:NF012731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HXC61919.1"
                     /translation="DTNKLVIREIPFSTTTESVIASIEEAARKRRIKIRSISDFTAAQ
                     VEILVTLSPGEEPEKAISALYHFTQCEVAIHSNIVVIKGERPVQMDVDEVIRFNTDQL
                     VSLLKQELELRRHELQDDLHWKSLIQLFIENRVYKGIEECETYEGVQQAVLKGLQPFR
                     TRLTRDVTIEDVEMLLSLQIKRISKFDLKKNEEDKLKINLELKEVERYLKTLTNYAID
                     YLQRLIGKYHKQYKRKTQLTTAEEIDVRELTANELAIAHDQERGFLGHQVKGEAAFRC
                     SPLDRLLLIWDNGRYQMIPPPDKLFVDKNLTYFGIFDRDKIFTLVYSTSKATFIKRFA
                     FGGAIMNRDYQCAQDNAKIRLLTDRPITEIRLKYRHAKGARTDEQSFGASDFPVRGPK
                     TRGNQVTKRTVTSVMGKG"
BASE COUNT          304 a          353 c          380 g          374 t
ORIGIN      
        1 agatgcgatc aagaacaagg agatccactt cctacgttat tgaatcatgc cctatgcggg
       61 acgctagcat ctgagaaatg gagaaagcat cggtgggaag ttggcttgta ccagccaccc
      121 gaaagacggc cgagggcgaa ggacgacgac gaggaggatt gggataactc ctccccccta
      181 tcccttcccc attacgctgg tgactgtccg tttggtcacc tggtttcctc tcgtttttgg
      241 accgcgaacc gggaaatcgc tagcgccgaa tgactgttcg tcggtccgag cacctttggc
      301 gtgccgatat tttaagcgga tctcggtgat cggacgatcg gtcaacagtc tgattttcgc
      361 gttatcctga gcgcattggt agtcgcggtt catgatcgcc ccgccaaagg caaaacgctt
      421 aataaaggtc gccttggagg tgctgtagac aagcgtgaat attttgtccc ggtcgaagat
      481 cccgaagtag gtgagattct tgtcgacaaa caacttatct ggaggcggga tcatctgata
      541 gcgcccgttg tcccagatta ggagcaagcg atcgagcgga gaacaccgaa atgcagcctc
      601 accttttacc tggtggccga gaaagccgcg ctcctgatca tgcgcaatcg ccaactcatt
      661 ggccgttagt tcccggacgt caatctcttc cgccgtcgtc agctgtgtct ttcgcttgta
      721 ttgcttgtgg tatttgccga tgagtcgttg aagatagtcg atcgcgtagt tggtcagcgt
      781 tttgaggtac cgctccacct ctttgagctc cagattgatc ttcagcttgt cttcctcatt
      841 ctttttcaaa tcgaatttgg agatccgttt gatctggagg ctcaagagca tttcgacgtc
      901 ttcgattgtg acgtcgcggg tcagacgggt tcggaagggt tggagccctt tcagcactgc
      961 ctgctgcacg ccctcgtagg tttcacactc ttcaattccc ttatagacac ggttttcaat
     1021 aaaaagttgg atcaatgatt tccaatgcaa atcgtcctgg agctcatggc gccgaagttc
     1081 gagctcttgt ttgagcaggg aaactaactg gtccgtgtta aatcggatga cttcgtcgac
     1141 atccatctgg accggacgct cgcctttgat caccacaata ttggagtgga ttgccacttc
     1201 gcattgggtg aaatggtaga gggcagagat ggccttctcc ggttcctctc cgggggacag
     1261 ggtgacgagt atttccacct gggctgccgt gaaatcgcta atagaacgaa ttttgatccg
     1321 ccgttttcga gccgcttcct caatcgatgc gatcacggat tcagtggtgg tactgaaggg
     1381 aatctctcga atgacaagtt tgttggtgtc c
//
LOCUS       DATMKH010000375         1909 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2410443, whole genome shotgun sequence.
ACCESSION   DATMKH010000375 DATMKH010000000
VERSION     DATMKH010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2410443"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(194..1381)
                     /locus_tag="VNV63_04530"
     CDS             complement(194..1381)
                     /locus_tag="VNV63_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HXC61920.1"
                     /translation="MVKMLSMGIWLFTAQNVFAASVSPCELKGSPPPSPRLQLESVAK
                     GFTEPLGLFHAGDGSGRVFIVEQPGTIRVLKNGRLSEKPFMDIRDRVTSGGETGLLGI
                     AFHPKFSENHRLFVNYTSSAGGLHTVISEFKAGDNPGDNPDKADPKSERVLLTIPQPY
                     PNHKGGNIVFGPDGYLYIGMGDGGSGNDPHGNGQNLSTLLGKMLRIDVNKKEKEKAYN
                     VPRDNPFIGKKNAAPEIWAYGLRNPWRYSFDPVTGLLYVGDVGQNAREEIDVIRRGKN
                     YGWNIMEGTICTPGVNPNCNKNGLELPIYDYPRSEGIAVIGGYVYRGRSIPNLCGAYV
                     YGDYGNGRIWALRYDEKFVTGHRLLLESHRSISSFGEDEQREIYVVDHGGEVLKIVPA
                     TLP"
     gene            1598..>1909
                     /locus_tag="VNV63_04535"
     CDS             1598..>1909
                     /locus_tag="VNV63_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HXC61921.1"
                     /translation="MNKKNYLFTSESVTEGHPDKISDQVSDAILDALIAQDPNCRVAV
                     ETLVTTGLAFVAGEITTTGYVEIPDIVRDTIKEIGYTRAKYGFDYETCSVITSIHNQS
                     PD"
BASE COUNT          453 a          510 c          485 g          461 t
ORIGIN      
        1 ataggtatgc tccctcatgt gatcgcgtct gatttcccgc aaactgcgct gcccgacaat
       61 agcacataaa tgcgggtcga aacaactgga agcaaggtcc ttttccaata tcctcatctc
      121 gctacttcaa aatgtcgtgc aaaatgtcgt gaagcgcgaa attgaaggcg aggagcgcga
      181 tgagggtaga ggatcaggga agcgtcgccg gaactatttt gagtacctcc ccaccatggt
      241 caaccacata aatctcgcgt tgctcatcct caccgaagga actgatgctc cggtggctct
      301 ccagaagcag ccggtgtcct gtaacgaatt tctcgtcata gcgtaacgcc cagattcgac
      361 cgttgccgta atccccatac acatatgccc cgcagagatt gggaatagag cggcctcgat
      421 agacatagcc gccgatgaca gcgattcctt cagaacgggg atagtcgtag ataggcagtt
      481 ccagcccgtt cttgttacag ttcgggttca cccccggcgt acagatcgtt ccctccatga
      541 tgttccatcc gtaatttttt cctcttcgaa tcacgtcaat ctcttcccgg gcgttctgac
      601 caacatcgcc gacatagagc agtccggtaa caggatcgaa cgagtagcgc cagggattcc
      661 ggaggccgta ggcccagatc tccggtgcgg catttttttt gccgatgaag ggattgtcgc
      721 gcgggacgtt gtaggctttt tccttctctt ttttattcac atcaatccgg agcatcttcc
      781 cgagcagcgt tgataagttt tgtccgttac cgtgcggatc gttacccgag ccgccgtcac
      841 ccattcctat atagaggtag ccgtccggac cgaagacgat attcccgcct ttatgattgg
      901 gatacggctg gggaatggtc agcaagaccc gttcactctt cggatcagct ttatccggat
      961 tgtcacccgg attgtcgccg gccttgaact cggagatcac ggtgtgaagg cccccggcgc
     1021 tggaggtgta gttcacgaaa aggcgatggt tctccgaaaa tttcggatgg aatgcaatcc
     1081 ccagaagtcc tgtctctcca ccggaggtca cccggtcgcg gatatccatg aacggttttt
     1141 ctgataatct cccgttcttt aaaactcgaa tcgtccccgg ctgctcgacg atgaaaaccc
     1201 tcccgctgcc atcgccggcg tggaaaagcc ccaacggttc cgtgaatcct ttcgcaaccg
     1261 attccaactg aaggcggggg gaaggagggg gagatccttt aagctcacag ggcgaaaccg
     1321 aagcggcaaa aacattttgt gcggtgaaga gccagattcc catactcaac attttaacca
     1381 tctgtttgcg gtctcctgta tgatgatttc ccattataaa agattaaatt tttcttgaca
     1441 agccagcggg gtgtgatgta gtgtaaataa aaattcgtgg tggattgatt taagcatatt
     1501 gcaaccacct taaaaaaagt gctaaaaagc cggggccagt cccgggttac cctgtgcttt
     1561 aaggcacagg gttttttatt gggaggttct catgagcatg aacaagaaaa attacctgtt
     1621 tacttccgag tccgtgaccg agggacaccc cgacaagatc tcggaccagg tctcggacgc
     1681 aatcctcgat gcactgatcg cccaagaccc gaactgccgt gttgcggtgg aaaccttggt
     1741 cacaaccggc ctcgcttttg tggccggaga gatcacgacc acgggctatg tcgagattcc
     1801 ggatattgtg cgcgacacga tcaaagagat cggctacacg cgggccaagt acggatttga
     1861 ttatgaaacc tgttctgtga tcacctcgat ccacaatcag tctccggac
//
LOCUS       DATMKH010000376         1407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2741503, whole genome shotgun sequence.
ACCESSION   DATMKH010000376 DATMKH010000000
VERSION     DATMKH010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2741503"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            213..1214
                     /locus_tag="VNV63_04540"
     CDS             213..1214
                     /locus_tag="VNV63_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003579096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXC61922.1"
                     /translation="MNYRRLGNAGVKVSDLSLGSWVTFGDQISDDVAEALMIKAYDGG
                     INFFDNAEGYASGRSELVMGKILRKLGWPRDTWLVSSKVFFGSAEHPQKPNQNGLSRK
                     HVFEACHAALKRLQVDYLDLFFCHRPDPETPIEETVRAMSDLIAQGKVLYWGTSVWSA
                     DEIMEAWTIARQFHLVPPTMEQPYYNLLQRDRVEVEYARLYDRIGLGTTIWSPLASGL
                     LTGKYNDGLPRDARLGLDHYGWLRDWVLKPERLEKVRRLGKLAAELGLSQSVLAIAWC
                     LKNPNVSTVILGASKVAQLEENLLASSAAEKLNPELMSQIDEIMGTKPKEASNSDNH"
BASE COUNT          342 a          345 c          390 g          330 t
ORIGIN      
        1 acgtgccagg catgcgatgt taaaatcacg gtcggatagt acgttgacgg tgcttcgcag
       61 cgcaacttaa aatagcggga ttggagcacg aggacaggag acagaattca gcatacagaa
      121 gctccaggag ttgaggattg gtattttcac gcataagaca ctcgcgtgtt ttttggcctc
      181 ccgccaaaca atgtgcaaac gtgagcgtgc ttatgaacta tcgcagactt ggtaacgctg
      241 gcgtgaaagt cagtgatcta tcccttggat cttgggtcac cttcggtgac caaatctctg
      301 atgacgtcgc agaagcctta atgatcaaag catacgatgg cggaatcaat tttttcgata
      361 acgccgaagg gtatgcttcc ggaaggtcgg aactggtcat gggcaagatc ctccggaaac
      421 tcggctggcc gcgggatacc tggctcgtct ccagcaaagt cttcttcgga tcggctgaac
      481 atccgcagaa gccgaatcag aatggactca gccgcaaaca tgtttttgaa gcctgccacg
      541 ccgcattaaa acggttgcag gtcgattacc tggacctctt cttttgtcat cggcctgatc
      601 cggagacgcc gatcgaagaa acagtccgcg ccatgtccga tctgatcgct cagggtaaag
      661 ttttgtactg gggaacgagc gtctggagcg ccgacgagat catggaagcg tggacgatcg
      721 cccgtcagtt tcaccttgtt ccacccacca tggagcagcc gtattacaat ctcctgcagc
      781 gcgatcgcgt cgaggttgag tatgcgcgtc tctacgatag aatcggtttg ggcacgacga
      841 tctggtctcc attggcgagc gggctactga ctggaaaata caatgatggg ctgccaaggg
      901 atgcccgtct tggtctcgat cactacggct ggctgcggga ctgggtttta aaaccggagc
      961 gcctggaaaa agtccgacga ttaggcaaat tggccgctga gcttggcctg tcccaatccg
     1021 tgctggcgat cgcttggtgc ctcaagaacc cgaatgtcag cacggtcatc ctcggagctt
     1081 cgaaagtggc tcagttggaa gaaaaccttc tggcttcgtc cgcggcagaa aaactgaatc
     1141 cggaactgat gagccagatt gacgagatta tgggcacgaa gccgaaggaa gcttcgaact
     1201 cggataatca ttgaagaaga agacaggaga cagaattcag gagttcaggg gcttcattct
     1261 gttcgccaaa tactctctgg gagagatctg tagcatcggc ggagattcgt agatttattc
     1321 tgcaactcct gtaactcctg gattctgaat tctggattct gaatgaattc ttctcctcgc
     1381 gactctttct tgacaatcgg cgatcgg
//
LOCUS       DATMKH010000377         2644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_175959,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000377 DATMKH010000000
VERSION     DATMKH010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175959"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..2538)
                     /locus_tag="VNV63_04545"
     CDS             complement(136..2538)
                     /locus_tag="VNV63_04545"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXC61923.1"
                     /translation="MKHAPSSIGFSTNGDKAESEQLAQRTAELFQDHCQQVWRRTDRM
                     FAGLMVLQWLAGIGLALWITPQTWIGATSSIHLHVPVAVLLGGTIAAFPILLVVLRPG
                     QVITRHVIAFSQTLASALLIHLTGGRIETHFHIFGSLAFLAFYRDWRVLGTATVVVAF
                     DHFLRGTFWPLSILGTVTPDSFRWIEHAGWVLFEDVFLFIMCRQSILEMRAIAGRQAE
                     LELTNGRIEIAVKERTRDLDQTNHELQKAKEKADAANLAKSSFLANMSHEIRTPMNGV
                     IGMTGLLLDTELHDEQIGIVETIEQSGESLLAIISEILDFSKIESGSLELEHLAFDLA
                     PCLEEVLDLFGVQSAEKNIDLAYLYDAHTPGAIVSDPTRLRQVLINLVGNALKFTEKG
                     EVVVEVSSERLSSRDIPQDNPFLRLLEEEKFEEEEWTLLRFEVRDTGPGIPADCMDRL
                     FQPFSQVDASITRKHGGTGLGLIISKRLVEAMGGKIWITSVTGTGTSFFFTLFTKATS
                     SGRRVNFLTSSAVLKDRRVLIVDDGEINRRSLRIQTERWGMSPQVFERPAEVLSWLEG
                     GPRVDVGILDFQMPIVDGCQLAREIHSLNRYKELPLILLSSSLPSRGMGLSPNDEFAV
                     RVMKPIKQADLFSALTTALGKIKTATKSLRQNKIFDSAMAARLPLNILIAEDNVVNQK
                     VAMGILLQFGYQADLVLSGKQAVEAVERQKYDLLFMDLQMPEMDGLEATRLICSRINP
                     SARPYIVAMTANATKEDHDLCLSAGMDDFLSKPIHPDEIKAAVERAAMKLSHILPESR
                     KS"
BASE COUNT          651 a          671 c          713 g          609 t
ORIGIN      
        1 ttggcctttg catggaagaa ccatagacga cgggatcgac ggttggatag agggaaccat
       61 cccggtgacc gggcacggcg accacacgat ctggattgta ccggcgaacg gggcagattt
      121 gatgtgatcc agaagctacg attttctcga ttcaggaaga atatggctca atttcattgc
      181 agcgcgttcg acggcggctt tgatttcgtc cgggtggatg ggcttgctga gaaagtcatc
      241 catccccgca gaaaggcaaa ggtcgtggtc ttctttcgtc gcattggcgg tcatggcgac
      301 aatataaggc cgtgcagaag ggttgatgcg tgagcaaata aggcgggtcg cttcgagacc
      361 atccatttct ggcatttgga ggtccataaa gaggagatcg tatttttggc gttccacagc
      421 ttccacagct tgctttccgc tgagaaccag atcggcttga taaccgaatt ggagaagaat
      481 gcccatggca actttctggt taacgacatt gtcctccgcg attaaaatat taagcggcag
      541 acgtgcggcc atggcggagt cgaagatttt attttgacgt aacgatttcg tagccgtctt
      601 gatttttccc agggccgtcg tgagcgcgct aaacaagtcc gcttgtttaa tgggtttcat
      661 gacacggact gcgaactcgt catttgggct caagcccatg cctctggaag gaagggacga
      721 actgagtaga ataagaggga gttccttgta tctgttgagc gagtggattt cccgggcaag
      781 ttgacacccg tcgacgatgg gcatttgaaa atctaggatt cccacgtcca cgcgaggtcc
      841 accctcaagc caggacaaga cctccgctgg tctctcaaag acttgggggc tcatccccca
      901 acgttcggtc tgaatgcgga ggctgcgacg attaatttcg ccatcatcaa cgatgagaac
      961 tcgccgatct ttcagcactg ccgacgaggt tagaaaattc actcgacgac ctgaactggt
     1021 ggctttggtg aatagagtga agaagaatga ggttcccgtg cccgttacac tggtgatcca
     1081 gatctttccg cccatcgctt caaccaggcg cttggaaatt atcaggccca agccggttcc
     1141 tccatgtttt cgcgttatgg aggcatccac ctggctgaaa ggctggaaaa gtcgatccat
     1201 acagtcagcg ggaataccgg ggccggtatc gcgaacttcg aaccggagga gtgtccattc
     1261 ctcttcttca aacttctctt cctccaagag ccgtagaaag ggattgtctt gagggatatc
     1321 ccggctggaa agccgctcgc ttgagacctc cacgaccacc tctccctttt ccgtaaattt
     1381 gagagcattg cctaccaggt tgattaggac ctgtcgcaat cgcgtggggt cgctgacgat
     1441 agcccccggc gtatgggcat catagaggta agcaaggtcg atatttttct cggcagattg
     1501 cacgccgaaa agatcaagca cctcttccag acaaggggcc aggtcaaagg ctaaatgctc
     1561 gagttccagt gagcccgatt caattttgga gaaatcgaga atctcactga tgatggccag
     1621 tagactctct ccgctttgct caatagtctc aacaatacct atctgctcat catggagctc
     1681 ggtatcgagt aatagccccg tcatcccgat aacgccgttc attggtgttc gtatctcgtg
     1741 actcatgttg gccaggaaag aagacttggc gaggttagcg gcgtccgctt tttccttggc
     1801 tttctgaagt tcgtgattgg tctgatccaa atcacgagtg cgctctttca ctgcgatttc
     1861 aattcggccg ttggtcaatt ccagttctgc ttgacgcccg gcgatggctc gcatttcgag
     1921 gatgctttga cggcacataa tgaaaaggaa aacatcttca aaaagcaccc aacccgcatg
     1981 ctcaatccag cgaaacgaat ccggtgtcac cgttcccaaa atcgatagcg gccagaaggt
     2041 gccgcgcagg aaatgatcga aagccactac aaccgtcgcg gtgccaagaa cacgccagtc
     2101 ccgataaaag gctaaaaacg ccagggaccc aaaaatgtgg aagtgcgtct caatccgtcc
     2161 tccagtgagg tgaattagca gcgcggaggc cagcgtctgg gagaaggcga tcacatggcg
     2221 ggtaatcacc tgccccggac gcaaaacaac tagcaggatc gggaaagcgg caatcgtgcc
     2281 tcccagaagg accgcgaccg ggacatgaag gtggatcgaa ctggtggctc caatccaggt
     2341 ctgaggagtg atccagagag ctaggccaat gcctgccagc cactgtaaca ccattaaacc
     2401 cgcaaacatg cggtcggtgc gccgccagac ctgctgacaa tgatcttgaa aaagttcggc
     2461 tgtccgttga gctagctgct ctgattcagc cttgtctccg ttcgttgaaa atccaataga
     2521 agatggagca tgtttcatga atatccttct agattgaacg gttgccgaga agtctcttta
     2581 caagagcgaa caaccgaaga agggtgtgtg tccaaccgga gtgtgaccgc tgagaataaa
     2641 gctg
//
LOCUS       DATMKH010000378         1987 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3680675, whole genome shotgun sequence.
ACCESSION   DATMKH010000378 DATMKH010000000
VERSION     DATMKH010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3680675"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1050
                     /gene="lysA"
                     /locus_tag="VNV63_04550"
     CDS             <1..1050
                     /gene="lysA"
                     /locus_tag="VNV63_04550"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HXC61924.1"
                     /translation="LIGKEGGGADIVSGGELYRALAAGIAPHKIVFAGVGKTAEEIRY
                     ALKTDILMFNVESSQELLQIDSVARELKLKARVALRVNPNIDPKTHPYISTGLKKNKF
                     GIAIEHALLEYQQAAKLDHIEIVGVHQHIGSQLTEVQPYVDALKRILSLVEELKQTGI
                     QIQYINVGGGLGITYDNEQPPLPADLAQALTPLVKDLKCKMILEPGRSIVGNAGILIT
                     KVLYTKEADDKRFVIVDAGMNDLIRPSLYSAYHKIQHVFQNQRKKVKVDVVGPICESA
                     DFFAKDRELEECQSGDLLAVMSAGAYGFSMASNYNSRPLAAEVMVKEDQYAVIRERED
                     YRDLIKGEKLPDFLS"
     gene            1075..1470
                     /locus_tag="VNV63_04555"
     CDS             1075..1470
                     /locus_tag="VNV63_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61925.1"
                     /translation="MNRPETTVESGIETRGRSFFIGLLSVSAVSILALLLMYYKGDFY
                     GKRVAVAAIGNIVAQDAFLGLLSSDFSPGQARQIQQKLAAFYLAAKQGQVPDEKLLNV
                     SEMLKAMLADNRLTKEEVEVILNRIEEKR"
     gene            1473..>1987
                     /gene="dapF"
                     /locus_tag="VNV63_04560"
     CDS             1473..>1987
                     /gene="dapF"
                     /locus_tag="VNV63_04560"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HXC61926.1"
                     /translation="MSRGKTITFFKMSGCGNDFILIDHRKKFLKDRELKRFAASICRH
                     RLSVGGDGLILLEKAKKKDADFSWRLFNADGSEAEFSGNGGRCAARLAYLLKIAPARM
                     AFETLAGLIQAKIHGENVTIQFPIPQNLGLGLKIPVDDKTYEGHFLNTGVPHLVYFVS
                     DLERTEVVPLGR"
BASE COUNT          480 a          520 c          569 g          418 t
ORIGIN      
        1 ctcatcggta aggaaggcgg cggggccgat atcgtttccg gtggagaact gtaccgagcg
       61 ctcgcggccg gcatcgcacc gcacaagatc gttttcgccg gcgtgggaaa gaccgcggaa
      121 gagatccgat acgccttaaa gacggatatc ttgatgttca acgtcgaatc ctctcaagaa
      181 ctcctgcaga tcgattccgt tgcgcgagaa ctcaaactca aggcacgggt ggcgctccgg
      241 gtcaatccca acattgatcc caagacccat ccctatattt cgaccggcct taaaaaaaac
      301 aagttcggga tcgcgatcga gcacgccctg ttggagtacc agcaggcggc caaactggat
      361 catatcgaaa tcgtgggcgt ccaccagcac atcggctctc agctcaccga ggtccaaccg
      421 tatgtggacg cgctcaagcg gatactctcg ctcgtcgagg agctcaagca gacggggatc
      481 cagatccagt atattaacgt cggaggaggg ctcggcatca cgtacgacaa cgagcagccg
      541 ccgctgccgg ccgatctggc ccaggccctg acccccttgg tgaaagatct caaatgcaag
      601 atgatcctgg agccgggccg ttcgattgtg ggaaacgccg ggatactgat caccaaggta
      661 ctctatacta aggaagcgga cgataagcga tttgtgattg ttgatgccgg gatgaacgat
      721 cttatcaggc ccagcctgta cagcgcgtat cataagatcc agcatgtctt ccagaatcaa
      781 cgtaaaaagg tcaaggtgga tgtggtggga ccgatctgcg aatcggctga tttcttcgcc
      841 aaggaccgtg aactggagga atgccaatcc ggcgatctct tggccgtaat gtcggcaggc
      901 gcctatgggt tcagcatggc ttcgaactac aactcccgcc ccctcgcggc cgaggtgatg
      961 gtcaaggaag atcagtatgc cgtgatccgt gaacgagagg actaccgcga cctgatcaag
     1021 ggagagaaac ttccggactt cctgagttaa cctgcacagc ttgagcaaac ccccgtgaat
     1081 cgtccagaaa ccacagtaga atccggcatc gagacaagag gcagaagttt ttttattgga
     1141 ctgctttcgg tcagcgcggt gagcatcctg gccctgctcc tgatgtatta caaaggtgat
     1201 ttttatggaa agcgggtcgc ggtcgcggca attggaaata tcgtggcgca ggatgcattt
     1261 ctcggacttc tttcatccga tttttctcca gggcaggccc gccagatcca gcaaaagctg
     1321 gccgccttct acctggcggc caagcaggga caggtcccgg atgagaagtt gctcaatgtg
     1381 agcgaaatgt taaaagcgat gctggccgac aaccgtctga cgaaagaaga ggtcgaggta
     1441 atcctgaacc ggatcgagga gaagaggtga ccatgtcccg cgggaaaacg atcacatttt
     1501 ttaaaatgag cggctgcggc aatgatttta ttctgatcga tcatcggaag aagtttttga
     1561 aagaccgcga actcaagcgg tttgccgctt ccatctgtcg tcaccgtctt tcggtcggcg
     1621 gggacggcct catcctcctg gaaaaggcga agaaaaagga tgctgatttc tcgtggcggc
     1681 tgttcaatgc ggacgggagt gaggcggaat tcagcgggaa cggcggacgc tgcgccgctc
     1741 ggctggctta tctcttaaaa atcgccccgg cccggatggc ttttgaaacc ctggcgggtc
     1801 tgatacaggc caagatccac ggtgagaatg tgacgatcca gtttccaatt cctcagaatc
     1861 tcggactggg gttaaagatc ccggtggacg acaaaactta tgaaggccat tttcttaaca
     1921 ccggggtccc tcatcttgtc tatttcgtat ccgacctaga gaggacggag gtcgttccgc
     1981 tgggacg
//
LOCUS       DATMKH010000379         1551 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3380209, whole genome shotgun sequence.
ACCESSION   DATMKH010000379 DATMKH010000000
VERSION     DATMKH010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3380209"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            194..1099
                     /locus_tag="VNV63_04565"
     CDS             194..1099
                     /locus_tag="VNV63_04565"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HXC61927.1"
                     /translation="MILAVFTGCQKTISVSPGARVLAAVNDEAITVGEFQKEMGQDSQ
                     TSEDPEVLKTLKHDLLQQLIDRKLFLQEAKHLKLSVSDEELAQASNQIQGDYTPEEFR
                     ELVDSKQLGDAVWKTGLKEKLLIEKLQNQATSNPKIDITMEEAKQYYDANRAHFIIKE
                     SVHVHQIVVAKEADAKAIRLELLNGADFATLASSKSLGPEKNQGGDLGFMTRDNLPEE
                     FDLIFSIGIGKISPVVKSPYGYHIFKVEERRSAHQETFVESIEKIQAVLLQIKQEQRF
                     ADWIADLRHRSDIKINEQLLNEVSG"
     gene            1096..>1551
                     /locus_tag="VNV63_04570"
     CDS             1096..>1551
                     /locus_tag="VNV63_04570"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HXC61928.1"
                     /translation="MKHGSRYKKFLEGAAAALILASMGAGFAVAAHAAPVTVDRIVAV
                     VNNEVITQSELEEAVAQAKAPPPQSQIQHETLKKMIEKKLQLQYAKKKGISVSQEELQ
                     AALQDIKKRNNFPTDQALNDALAAEHLTFEQYKTDLMEQLLLLKLVNREV"
BASE COUNT          454 a          384 c          398 g          315 t
ORIGIN      
        1 gtgatccggc aagaagacct gaaacgcctg atgggccgct tcccgggcca gcttcgtttt
       61 ctatccgaat acgcctttcg cctgccggtg gcggaggacc gatgggagag cacggccaat
      121 cagatcagga gttgtcttcg aacactgtca gaaacggtaa tggaccatgt ctaatagaat
      181 ctgtttattg gggatgattt tagcagtctt caccggatgc caaaagacga tctcagtctc
      241 tcccggcgcc agggttctgg ctgcggttaa tgatgaagca atcacggtcg gcgaattcca
      301 aaaagaaatg ggacaggatt cccaaacctc tgaagaccct gaggtgctca aaaccctcaa
      361 gcacgatctc cttcaacagc tcattgaccg aaagcttttt ctgcaagaag caaaacacct
      421 aaagctctcg gtgagcgatg aggaattggc gcaagcatca aaccaaattc aaggagacta
      481 taccccggag gagtttcggg aactcgtcga ttcaaaacag ctcggcgacg ctgtctggaa
      541 gaccgggctg aaagaaaaac tgctcattga aaaactacaa aaccaggcca cgagtaatcc
      601 aaagatcgat attacgatgg aagaagcaaa gcaatactat gacgccaacc gggcacactt
      661 tatcataaaa gaatcggttc atgtccatca gattgtggtg gcaaaagagg ccgacgctaa
      721 agcaattcgg ctggagctcc tgaacggagc cgattttgca acgctggcct cttcaaaatc
      781 actgggcccg gaaaagaatc agggagggga tctcggattt atgaccagag acaacctccc
      841 ggaagaattt gatttgattt tttccatcgg gatcggtaag atcagccccg tggtcaaatc
      901 tccttacgga taccacattt tcaaggtaga agagagaaga tcggctcacc aagaaacctt
      961 tgtcgagtct atcgagaaga tccaggcggt tctgctccag ataaaacagg agcagcgttt
     1021 tgcggactgg atcgccgatc tccggcatcg aagcgacatt aagattaatg aacagcttct
     1081 caacgaggtg tcgggatgaa acacggaagc cgatataaaa agtttttgga aggagccgcg
     1141 gctgccttaa tcctggcatc catgggagct ggcttcgcag tcgctgccca tgcagctccc
     1201 gtaacagttg atcgcatcgt ggcggtggtg aataatgagg tgatcaccca gtcagaactg
     1261 gaggaggcgg tggcccaggc caaggctccg ccaccacaat cccagattca acacgagacg
     1321 ctcaaaaaga tgatcgagaa gaaattgcag cttcagtacg ccaagaaaaa aggcatctcg
     1381 gtcagccagg aagaacttca ggccgcgttg caggacatca agaagcgaaa caacttcccc
     1441 actgatcagg cgctcaatga tgccttggca gctgaacacc taacgtttga acaatacaaa
     1501 acagacctca tggagcagct cctcctcttg aagctggtaa atcgagaggt a
//
LOCUS       DATMKH010000380         1212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2742411, whole genome shotgun sequence.
ACCESSION   DATMKH010000380 DATMKH010000000
VERSION     DATMKH010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2742411"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..>1212)
                     /gene="ureC"
                     /locus_tag="VNV63_04575"
     CDS             complement(63..>1212)
                     /gene="ureC"
                     /locus_tag="VNV63_04575"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010871711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HXC61929.1"
                     /translation="EYPVNLGFLGKGNSSMPESLREQIEAGAIGLKLHEDWGTTPAAI
                     DCCLKVADEYDIQVAIHTDTLNEAGFVEDTIAAFKGRTIHTYHTEGAGGGHAPDIIRV
                     CGESYVLPSSTNPTRPLTVNTISEHLDMLMVCHHLDPRIPEDVAFAESRIRQETIAAE
                     DILHDLGALSMMSSDSQAMGRVGEVIIRTWQTADKMKKQRGALKGEKGANDNGRAKRY
                     IAKYTINPAIAHGISEHVGSLEIGKLADLVLWKPAFFGVKPEMVIKGGFIACAEMGDA
                     NASIPTPQPVLLRPMFGAMGKARYSTSFTFLSQAAIRRGIGKKLRLSKMVVPVKNCRE
                     LKKKDMKHNSYLPKIEVDPETYTVRADGQLLTCESASVLPMAQRYFLF"
BASE COUNT          273 a          349 c          285 g          305 t
ORIGIN      
        1 tcttctatcc aatagaccta gcattcatcg aaactccatc catgcatgag gccggatata
       61 tatcaaaaaa gaaaatatct ttgggccatg ggaagcacgc tggccgactc gcaggttaac
      121 agctggccgt cggcccggac cgtataggtt tcagggtcca cctcaatctt gggaagatag
      181 ctattgtgtt tcatatcttt tttcttgagt tcccggcagt tcttcaccgg aacgaccatc
      241 ttgctaagcc tgagtttttt accgatcccg cgccgtatcg ctgcctgaga gagaaatgta
      301 aagctggtcg aataacgagc cttgcccatc gcgccaaaca tcggtctcaa gagaacaggc
      361 tggggagtgg ggatcgaggc attggcatct cccatctcgg cgcaggcgat aaatcctccc
      421 ttgataacca tctcgggttt gacaccaaaa aaagccggtt tccacaaaac cagatcggcc
      481 agcttgccta tctcgagcga tcccacatgc tctgagatac catgggcaat ggcagggttg
      541 atcgtatatt tggctatata tctcttggct ctcccattgt cattcgcccc tttttccccc
      601 ttgagagcgc ccctctgttt cttcatcttg tcggcggtct gccatgttcg aatgatcacc
      661 tctcccacac gccccatcgc ctgagagtcc gagctcatca tactcaaagc gcccagatca
      721 tgcagtatgt cctctgcagc aatcgtctct tgccgtattc ttgattcggc aaaggccaca
      781 tcctccggaa ttctgggatc gagatggtgg cagaccatga gcatatcgag atgttcactg
      841 atcgtattca cggtcaaagg tcttgtggga ttcgtcgagg agggcaggac atatgactct
      901 ccgcagaccc gtatgatgtc aggagcatgg cctccgcccg caccctctgt atgataggta
      961 tggatcgtcc tccctttaaa agccgcgatg gtgtcctcca caaaacccgc ctcgttgagc
     1021 gtgtccgtat ggatcgcgac ctgaatatca tactcgtcag caaccttgag acaacagtcg
     1081 atggcggcag gcgtcgtccc ccaatcctca tgaagcttca agccaatcgc accggcttct
     1141 atctgttctc tcagagactc gggcattgac gagttcccct tccccaggaa acctagattg
     1201 accgggtatt ct
//
LOCUS       DATMKH010000381         2756 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3681777, whole genome shotgun sequence.
ACCESSION   DATMKH010000381 DATMKH010000000
VERSION     DATMKH010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2756
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3681777"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..855)
                     /locus_tag="VNV63_04580"
     CDS             complement(<1..855)
                     /locus_tag="VNV63_04580"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXC61930.1"
                     /translation="MDMLQKLAILGEGAAQEQELTLNQSSRPACFTPQQITDPYQRHR
                     NPVMKGPNRFLPGGIHASPLPGGGSIRLLKVLQTNICEFDCFYCEHRASRDVPRTYVS
                     PDELAQTFMMLHQKHLVEGIFLSSGITKKIDTMQERMVQTVEILRKKYQFKGYVHLKI
                     LPGTSAAAMEQSLGLADRVSINLEAPNPTRLKKLSHIKDFIGMLAQLKWIKDQKLARP
                     NLIRAGVTTQFVIGTANESDQEILSTTDRLYQDLNLRRAYFSAFSPIPDTPLKGHPPA
                     SLIREHRLY"
     gene            complement(879..1127)
                     /locus_tag="VNV63_04585"
     CDS             complement(879..1127)
                     /locus_tag="VNV63_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61931.1"
                     /translation="MITEVDETVRVGAVFDKSQTIFPRWFVWHGRKYLIRRVTFSWKV
                     KEGINLNYHFSVTDGVNLFELCYKPQALAWKLMSVSPM"
     gene            complement(1127..1744)
                     /gene="lexA"
                     /locus_tag="VNV63_04590"
     CDS             complement(1127..1744)
                     /gene="lexA"
                     /locus_tag="VNV63_04590"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HXC61932.1"
                     /translation="MPAELTERQKNIFSFLKDFLKKKGYPPTLREIAGHFKMAGPKGA
                     KKHLDRLAAKGYIKKLPGCSRAIEILTDSPLTPARMIPIVGRVRAGSPILAEEQIEGL
                     LAVDEDMAPWKEAFLLRVTGDSMIQAGILEGDYVLVKPQSTAENGETVVALIGDEATV
                     KRFYRDRDHIRLEPANDQLKPLIIAQASGEFRILGKVMGLIRRSV"
     gene            1901..2485
                     /locus_tag="VNV63_04595"
     CDS             1901..2485
                     /locus_tag="VNV63_04595"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HXC61933.1"
                     /translation="MNDVPSQKASLRTLLLQRRERLSPEAVHRKSEAIRQRLFALDEF
                     RAAGLIHFFIAIGREVETLPMIQEAITLQKRVVVPVVVPESMDMRLSELLKDSPLVQG
                     VRGTLQPSPDQIRPINTSEIDLMLVPGMAFDRHGHRLGRGSGYFDRLLGQSFKKSMPI
                     IALAYELQLVEHIPVMDHDKKVDKIITEERVIDC"
     gene            2532..>2756
                     /locus_tag="VNV63_04600"
     CDS             2532..>2756
                     /locus_tag="VNV63_04600"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009305704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HXC61934.1"
                     /translation="MDMKKIEEGVRLILEGIGEDPNRPGLKETPQRVAKMYAEIFAGL
                     NASAELLLRPISGESHDEMVLIKDIPFYSVC"
BASE COUNT          607 a          694 c          748 g          707 t
ORIGIN      
        1 atacaggcgg tgctctcgta tcagagatgc cggcggatgg ccctttaacg gtgtatccgg
       61 gatcgggctg aaggcgctga agtaggcacg ccggagattt aaatcctgat acaaccggtc
      121 tgtggtggag agaatctctt gatccgattc gttggctgtt ccgattacga actgagtggt
      181 aaccccggcc cggatcagat ttgggcgggc cagtttctga tctttaatcc atttgagttg
      241 agccagcatg ccgataaaat cctttatgtg ggaaagcttc ttcaggcgtg ttgggttcgg
      301 tgcctccagg ttgatcgaaa cgcgatcggc cagccccagg ctttgctcca ttgcggcggc
      361 gctggtaccg gggaggatct tgagatggac atagcctttg aactgatatt ttttccggag
      421 tatctcaacc gtctggacca tccgttcctg catcgtgtca atcttttttg tgatgccgga
      481 agagagaaaa atcccctcga ccagatgctt ctggtgaagc atcatgaagg tctgggccaa
      541 ctcatccggc gagacgtagg tcctgggaac atcccggctg gctcggtgct cacagtaaaa
      601 gcagtcaaac tcgcagatat tggtctggag gactttgagg agccgaatac ttccgccacc
      661 cgggagcggg ctggcgtgga tcccaccagg caggaatcgg ttaggtcctt tcatcacagg
      721 gtttcggtga cgttggtagg gatcggtaat ctgctgcggc gtgaagcagg caggtctgct
      781 actctgattt agcgtgagtt cctgctcctg cgccgctcct tctcccagaa ttgcaagttt
      841 ttgtagcatg tccatgatat tttcctcttc ttcgctcgct acatcgggct taccgacatt
      901 agcttccagg cgagagcctg cggcttatag cagagctcga acaggtttac gccatccgtc
      961 actgagaagt gataatttaa gttgatacct tccttcacct tccaggaaaa agtcacccga
     1021 cggatgagat acttccggcc atgccacaca aaccagcgcg gaaagatcgt ctggcttttg
     1081 tcaaacactg caccgaccct caccgtttca tccacttctg taatcatcaa acgctccttc
     1141 gaatcagccc catcaccttc ccaaggatcc gaaattctcc ggatgcctga gcgattataa
     1201 ggggtttaag ttgatcattg gccggttcta agcggatatg atctctgtct cgatagaacc
     1261 gtttgaccgt ggcctcgtct ccgatgaggg ctaccacggt ttcgccgttt tcggccgtag
     1321 actgcggttt caccaagaca tagtcgcctt ctaagattcc ggcctggatc atgctgtccc
     1381 ccgttacacg gaggagaaag gcctccttcc agggtgccat gtcctcatcc acagcgagaa
     1441 ggccttcgat ctgttcctct gccagaatcg ggctgccagc gcgtacccgc cccacgatcg
     1501 gaatcatccg ggccggggtg aggggagaat cggtcaagat ttcgattgcc cgagagcatc
     1561 cggggagctt cttgatgtaa cccttggcgg ccagccgatc caggtgtttc ttggctccct
     1621 tcggccctgc catcttaaag tggcctgcga tctcccgaag ggtgggagga tagcccttct
     1681 tctttaagaa atcttttaag aaactgaaaa tgttcttctg tctctcggtt aattcggccg
     1741 gcattgtgtc ccctctaagg tctacaaatg tagaccttat tataattatc ggttttagtc
     1801 ctgtcaagag gtttttgatg cgggagataa atgcttgaca agccaatcgg gctcttgtta
     1861 ttctcgatct ctcaataatc aaagattttt ctgaagcata ttgaacgatg ttccatccca
     1921 gaaggccagc ctgagaaccc ttctcctcca gcgcagggag aggctttctc ctgaggccgt
     1981 gcatcggaag agcgaggcga tccgacagcg actgttcgca ttggacgagt ttagagccgc
     2041 aggcctgatc cattttttta ttgcgatagg gcgcgaggtt gaaaccctgc ctatgatcca
     2101 ggaggcgatc actttgcaaa aacgggtcgt cgttccggtc gttgtaccgg agagtatgga
     2161 tatgcgccta tccgagttgt taaaagatag cccgttggta caaggagtgc ggggaacgct
     2221 tcagcccagt cccgaccaga tacgtccaat taatacgagc gagattgatc tgatgctggt
     2281 ccccggtatg gccttcgatc ggcacggcca ccggctgggg cgagggtccg gatattttga
     2341 tcgtcttctg ggccaatcgt tcaaaaaatc gatgccaatc attgcattgg cttatgaact
     2401 gcaattagtc gagcacatcc cggtgatgga ccatgataag aaggtggata agatcatcac
     2461 ggaagagcgt gtgattgatt gttgagccgg tcgtgtttta aacagcagga tggtttgagg
     2521 aattcggtct tatggacatg aaaaagattg aagaaggagt tcggttaatt cttgagggaa
     2581 ttggagagga cccgaatcgg ccggggctca aggaaacccc gcaacgggtg gccaagatgt
     2641 atgccgagat ctttgccggt ctcaatgcct ctgccgagct tttgctccgg cctatcagtg
     2701 gcgagagtca tgacgaaatg gtcctgatta aagacatccc attttattcg gtctgt
//
LOCUS       DATMKH010000382         1928 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3060096, whole genome shotgun sequence.
ACCESSION   DATMKH010000382 DATMKH010000000
VERSION     DATMKH010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3060096"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1605)
                     /locus_tag="VNV63_04605"
     CDS             complement(<1..1605)
                     /locus_tag="VNV63_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HXC61935.1"
                     /translation="MNRIIKKVAVLGSGVMGSRIACHFAGIGVQTLLLDIAPKELTPA
                     EAAKNLSLEHPAVRNRIVNEALAAAIKSNPSPIYTKDVVSRIKTGNFVDNMKDITGCD
                     WVIEVVVERLDIKKQVFDEVEKYRRPGTLITSNTSGIPIHMMADGRSEDFRRHFCGTH
                     FFNPPRYLRLLEIIPTPATDPAVTEFLLHYGDVYLGKTTVLCKDTPAFIANRIGVYGI
                     MGIFGLLDKLGLTIDEVEALTGPLIGRPKSATFRTADVVGIDTLVKVAHGVAENCPND
                     EQRDVFRIPAWLDKMVANKWLGDKTGQGFFKKTKGTGGEKEILTLDLKRMEYGPRQKP
                     KFASVEAAKPVDDLKTRLKILFAGSDKAGEFIRLFHYGLFSYISHRIPEISDELYRVD
                     DAMMAGFGWEIGAFESWDVFGVEKTISQIKAAGFTVAPWVDEMLAAGIPAFYKIANGQ
                     RLCYDVASRSYKPLPGGEAFLVMKNFEGQTVWENSACHLYHLGDEVLGLEWNTKMGTI
                     GGEVLEGIQRGIGIAEEKYKGLVIAGD"
     gene            complement(1716..>1928)
                     /locus_tag="VNV63_04610"
     CDS             complement(1716..>1928)
                     /locus_tag="VNV63_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61936.1"
                     /translation="SPGSYRGRDKIHMTDICGNKIIIEKGVYLTVSYITADSYPRLLM
                     EEQIKQQTELINKADNFEIDRLSKIL"
BASE COUNT          414 a          569 c          466 g          479 t
ORIGIN      
        1 atcccctgcg atcacgaggc ccttgtattt ctcttccgcg attccgatcc cccgctggat
       61 cccctccagc acttcgccgc cgatcgtacc catctttgta ttccattcga gccccaggac
      121 ctcatcaccg agatgatata aatggcaggc gctgttctcc catactgtct ggccctcgaa
      181 attcttcatg accaggaagg cttccccgcc gggcagcggc ttatagctcc ggctggcgac
      241 atcataacaa agccgctggc cattcgcgat cttatagaag gcagggatac ccgccgccag
      301 catctcgtcc acccatggcg ctacagtaaa accggctgcc tttatttgcg agatcgtctt
      361 ctccaccccg aatacatccc agctctcgaa agcaccgatc tcccagccga atcctgccat
      421 catcgcgtca tctacccggt acaactcatc ggagatctcg ggaatgcggt gcgaaatata
      481 ggagaacaat ccataatgga aaagccggat gaattccccg gctttatctg acccggcaaa
      541 caaaatcttc agtcgcgtct taaggtcatc caccggcttg gcggcctcca ctgacgcgaa
      601 ctttggtttt tgccgcggcc cgtattccat cctcttcaga tcgagtgtga ggatctcttt
      661 ttccccgccc gtccctttgg tcttcttaaa gaaaccctga ccggtcttgt cgcccagcca
      721 cttgtttgct accatcttgt ccaaccaggc cgggatccgg aagacatccc gttgttcatc
      781 gttggggcaa ttttccgcga caccatgcgc cactttgacc agggtgtcga tccccactac
      841 atctgctgtc cggaatgtcg ctgatttggg gcggccgatc agcggacctg tcagcgcttc
      901 gacctcgtcg atcgtcaatc ctaatttgtc cagcaggcca aagatcccca tgataccata
      961 tactccgatc cggttggcga taaaggccgg ggtatccttg caaagcaccg tcgtcttgcc
     1021 caggtacacg tctccgtaat gcagcaggaa ttcggttacc gccggatcgg tggccggtgt
     1081 agggatgatc tccagcagcc gcagataacg cggcgggttg aaaaagtgcg tgccgcaaaa
     1141 atgtcgcctg aaatcttcgc tccttccatc cgccatcata tgaatgggaa tacccgacgt
     1201 gttggaggtg atcagcgttc ccggccggcg gtacttctct acttcgtcaa acacttgttt
     1261 tttgatatcc aaccgctcta ctaccacctc gatgacccag tcacaaccgg tgatatcttt
     1321 catattgtcg acaaagttgc cggtcttgat ccgcgaaacg acatctttgg tatagattgg
     1381 tgacggattg ctcttgatgg ctgccgccaa tgcttcattt acgatccggt tccgtaccgc
     1441 cggatgttca aggctaaggt tcttcgctgc ttcggccggg gtcagctcct tcggtgcgat
     1501 atcgagcaaa agggtctgaa caccgatgcc tgcaaaatgg caggcgattc tcgaccccat
     1561 caccccactc cctaatacag ctactttctt gataatgcgg ttcatggtct tcggttagtt
     1621 agttaaacaa ctatccgaag ctaaggtagg gaaaaaattc ttaccggccg aaaatcgcgg
     1681 cggggagacg tccgaaaatc acggtggaaa agcggttaca ggatcttgct caggcggtcg
     1741 atctcaaaat tgtccgcctt gttgatgagc tcggtctgct gcttgatctg ctcttccatg
     1801 agcagacgcg gatagctgtc agcggtgata tagctgactg tgagataaac acctttttcg
     1861 atgatgatct tattgccgca gatatctgtc atatggatct tgtccctccc gcggtaggag
     1921 ccagggga
//
LOCUS       DATMKH010000383         2561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1141063, whole genome shotgun sequence.
ACCESSION   DATMKH010000383 DATMKH010000000
VERSION     DATMKH010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1141063"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="VNV63_04615"
     CDS             <1..518
                     /locus_tag="VNV63_04615"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HemK/PrmC family methyltransferase"
                     /protein_id="HXC61937.1"
                     /translation="LDLCTGSGCIAITVAKYAVSARLYATDLSLQALGLAQANARKHD
                     LGKRITFLCGDLFEPLMTLGLEGRADLILSNPPYIPTDLIQGLQPEVSLYEPRQALDG
                     GQDGLEIIRRLIIQAPSYLSSRGRLLLEIGDQQAEPLTQWLQDLYPKAVLDFRLDLAG
                     IKRVACIDFKT"
     gene            522..1775
                     /gene="murA"
                     /locus_tag="VNV63_04620"
     CDS             522..1775
                     /gene="murA"
                     /locus_tag="VNV63_04620"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HXC61938.1"
                     /translation="MDKIVITGGKRLQGEVAIGGAKNAALPILASTLLSSGEYHIRRV
                     PKLMDVTTIRRLLTQLGAKIFDEGDGLAVEMNHVTSCEAPYELVKTMRASVLVLGPLA
                     ARMGEAHVSLPGGCAIGARPVNLHLDGLEKMGAEVKIEHGYVRVKAKRLKGATIHLDM
                     PTVTGTENLMMAATLAEGTTRIENAACEPEVVDLARFLEKRGAKITGAGTSAITIEGT
                     EKLNGADYEVMPDRIEAGTYLAAGAVTGGDVLVSGCAPSYLDAVLLKLREAGVKLKED
                     AKGIRVWGPKRLKAVDVKTLPYPGFPTDMQAQMMAVMSFASGLSVITETVFEGRFGHV
                     AELRRMGADIRVQGSQAVVQGVPGLTGAPVMASDLRASASLIIAGLAAEGTTEISRVY
                     HLDRGYERIEEKLGQLGVTVSRRKE"
     gene            1775..2428
                     /gene="hisG"
                     /locus_tag="VNV63_04625"
     CDS             1775..2428
                     /gene="hisG"
                     /locus_tag="VNV63_04625"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HXC61939.1"
                     /translation="MKETMTIALPKGRLLAPAIRLFERCGMKHRGLKTDTRSLVFESP
                     GQDWRFVIMRDRDVPTYVEYGAADMGIVGKDVLLEEDRDVYEPLDLKFGDCRLVLAQP
                     NGLGKSRRFRSLSSKTRVATKYPKITERYFSEKGLPVEIIKLYGSIEIAPWAGLAEQI
                     VDLIATGRTLRENRLSVVETIAESTARLIVNRASLKLKYQQVTELIHRLREKTEATR"
BASE COUNT          593 a          639 c          781 g          548 t
ORIGIN      
        1 tcctcgacct ctgtaccggt agcggctgca ttgcaattac ggtggccaaa tatgcagtct
       61 ccgcccgtct ctatgcaacg gacctttctc tccaggcttt agggctggca caagccaatg
      121 cgagaaaaca cgacctgggc aaacggatca cttttttgtg cggtgatcta tttgagccat
      181 taatgacttt gggattggag gggcgggcag atctgatttt gtccaatcca ccgtatattc
      241 cgaccgactt gatccagggc ttgcaacctg aagtctctct ctacgaaccg agacaggcgc
      301 tggatggggg ccaagacggg ttggagatca ttcgcaggct catcatccaa gcaccttcgt
      361 atctttcgtc acgaggcagg ctacttcttg aaattgggga tcagcaggcc gagcctttga
      421 ctcagtggct tcaggatctc tatcctaaag cggtcttgga tttccgactc gatctggccg
      481 gaatcaagag agtggcttgt attgatttta aaacatgaag catggataag atcgttatca
      541 caggtggaaa gcggcttcaa ggggaagtcg ccatcggtgg cgcaaagaat gcagccctgc
      601 ctattcttgc ctcgacactg ttgagttcag gagagtatca tatccggcgt gtccccaaac
      661 tgatggacgt aacgaccatc cgccgtctgc tgacccaact cggcgcaaag atctttgacg
      721 agggagacgg gctggctgtt gagatgaatc atgtgacaag ctgcgaggcg ccctatgagc
      781 tcgtaaagac catgcgtgcc tcggttctgg ttctgggacc tctcgcggcc cggatgggag
      841 aggcccatgt ctcacttccc ggcggctgcg caatcggagc ccggccggtt aatctccacc
      901 tcgatggatt ggagaagatg ggtgcagagg tcaaaatcga gcatggttat gtcagagtga
      961 aggcaaaacg attaaaaggc gccacgatcc atctggatat gccgaccgtg accgggacgg
     1021 aaaacctaat gatggccgcc acactggctg aaggcacgac ccggatcgag aatgcggctt
     1081 gtgagccgga ggtggtggat ctcgcccggt ttcttgaaaa acgcggggcc aagatcaccg
     1141 gggccggaac gagcgctatc acgatcgagg gaaccgaaaa acttaatggt gctgattatg
     1201 aggtcatgcc ggatcggatc gaggcgggaa cctatcttgc ggccggagct gtcaccggtg
     1261 gagatgtcct cgtaagcggc tgcgcaccga gctatctcga tgcggttctt ctgaaactgc
     1321 gtgaggctgg ggtgaagtta aaagaagatg ccaaagggat tcgtgtctgg ggtccaaaac
     1381 gactcaaggc ggtggatgtt aaaacgttgc cgtatcccgg ctttccgacc gatatgcagg
     1441 cccagatgat ggcggtgatg tcttttgcga gtgggctcag cgtgatcacc gagactgtct
     1501 tcgaaggacg gtttggacat gtggctgagt tgcggcgtat gggagccgat atccgagttc
     1561 aaggaagcca ggcggttgtg caaggggttc caggcctgac cggggcgccg gtcatggcat
     1621 cagacctaag ggccagcgct tccttgatca tcgccggtct tgcggccgag gggaccacgg
     1681 agatatcgcg tgtctatcac ctagatcgcg gctatgagcg gatcgaggaa aaactcggcc
     1741 agcttggtgt aaccgtctct cgcaggaaag aatgatgaaa gagaccatga ccatcgccct
     1801 tcccaaaggc cggttgctgg cacccgccat ccggctgttt gagcggtgcg gaatgaagca
     1861 ccgggggctt aaaacggata cgcgtagcct ggtttttgaa agccctggtc aagactggcg
     1921 gtttgtgatc atgcgggatc gagatgtccc aacctatgtg gaatacggcg cggctgacat
     1981 ggggatcgta gggaaggatg ttctgctgga agaggatcgg gatgtctatg agccgttgga
     2041 ccttaagttt ggtgactgcc gcttagtcct tgcacagccc aacgggcttg gaaaaagccg
     2101 ccggttcaga tccctgtctt ccaagacccg ggttgcgacg aagtatccta agatcacgga
     2161 acgatatttc agcgaaaagg gactgcccgt ggagatcatc aagctgtacg gctcaatcga
     2221 gattgccccc tgggcaggcc tcgccgagca gattgtggac ctcattgcga ccggtcgcac
     2281 tctccgcgag aatcgcctca gcgtggtgga aacgattgct gaatccacgg cacgactgat
     2341 tgtaaaccgg gccagcctga aactaaaata tcagcaggtc acagagttga tccaccgtct
     2401 tcgggaaaag accgaggcca ctcggtgagg ctacgatgaa gctgattcga ggagagaaaa
     2461 aaaagcttga ggctgcaatc cagcaggtgt cgaagcgatt tgatctcggc aatcggcgga
     2521 tactgcccca ggttgaagcg atcctaaaag cggttagaca g
//
LOCUS       DATMKH010000384         1174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4011913, whole genome shotgun sequence.
ACCESSION   DATMKH010000384 DATMKH010000000
VERSION     DATMKH010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4011913"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /locus_tag="VNV63_04630"
     CDS             <1..637
                     /locus_tag="VNV63_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXC61940.1"
                     /translation="KVKILLADDHLLVAEALKSLLVPTYDVVGTVSDGIALLEAAGAL
                     RPDLIVIDIAMPKLNGLDATRQLKRLLPRIKIIILTMNEDTDLVGEAFRAGASGYLLK
                     HSASGELMQAIHEVLKGSSYLSPRITGGAVASLLRGPDMDSGQSRELTSRQRQVIQLL
                     AEGRSMKEIADILTISLRTVAAHKYRIMEMLEIKTNAELYRYAVKHRIISV"
     gene            763..1092
                     /locus_tag="VNV63_04635"
     CDS             763..1092
                     /locus_tag="VNV63_04635"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXC61941.1"
                     /translation="MQDPTKSELPSVLIADDHFTVLESVVPMLKTFFNVVGTASDGKA
                     AVEAENRLHPDVVVLDISMPVMSGLDAATQMRKHGSKTRIVFLTVHEDTDILAAAKAA
                     WGKGYVV"
BASE COUNT          293 a          298 c          305 g          278 t
ORIGIN      
        1 caaagtaaag attctgttgg cggacgatca tctgttagta gccgaggctc tcaagagcct
       61 gctcgtgcct acatacgatg ttgttggaac ggtgagcgac ggcatcgcct tgctggaggc
      121 cgctggcgct ctcagacccg atttgatcgt gatagatatc gccatgccca aattaaatgg
      181 gcttgatgcc acgcggcagc ttaagcgcct attgcccagg atcaaaatca tcattctcac
      241 gatgaatgaa gatactgatc tggttgggga agcattcaga gcgggtgcat ccggatatct
      301 cctcaagcac tcggcctccg gagagttgat gcaagccatt catgaggtgc tcaaaggctc
      361 gtcgtatctt tccccgcgaa tcactggagg agcggtcgcc tctttgcttc gcggcccgga
      421 tatggattcc ggacaatcgc gggaattgac ctcgcgccag cgtcaggtca ttcaactgct
      481 ggccgagggg cgttcgatga aggaaatcgc cgatatcctc acgattagcc tgcgaacggt
      541 ggctgcccat aagtacagaa tcatggaaat gctggagatc aagacaaacg cggaactcta
      601 ccgctatgca gtgaagcatc ggattatctc ggtatagctt gcaaaaaaaa ccaaccaggt
      661 cctgtcagtt ttactactag cggtggccca tcactcctcc tatgatcctc atgtcgaccc
      721 tgaactagca gggtttttgt tttttcgaga tgagttgaga gagtgcaaga cccgacaaaa
      781 tccgagttgc cgagcgttct catagccgat gatcatttca cggtactcga atccgttgtg
      841 cccatgctca aaaccttctt taacgttgtg ggaacagcct ctgatggtaa agctgctgtc
      901 gaggccgaga atagactgca tccggacgta gtcgtgttgg atatttctat gcccgtcatg
      961 agtgggctcg atgcagccac acaaatgagg aaacacggat cgaagaccag aatcgtattt
     1021 ctcaccgtgc atgaagacac agatattctt gcggcagcaa aagctgcttg gggcaagggc
     1081 tatgtcgtat agtatcgctc gcctatttcg accaggcacc cttcgccttc aatggaaaga
     1141 tcatcacgac gaagatctcc tatccaaaga aaga
//
LOCUS       DATMKH010000385         3412 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_180120,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000385 DATMKH010000000
VERSION     DATMKH010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3412
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180120"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="VNV63_04640"
     CDS             <1..441
                     /locus_tag="VNV63_04640"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXC61942.1"
                     /translation="LALIRYYAEKSNILIKEEYEEVPKVSVDANQMKQVFINLIKNAM
                     EAMPEGGHLRIKTRWLLKEQQVEILFEDSGSGIHQEHLPKIFDPFFTTKGETVGTGVG
                     LAVSAGIIKRHGGEVSVESTVGQGSRFYVRLPLEAERVEKEALR"
     gene            438..1931
                     /locus_tag="VNV63_04645"
     CDS             438..1931
                     /locus_tag="VNV63_04645"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HXC61943.1"
                     /translation="MKNQRILVVDDEPFIIELCQRFLTAAGYTVQTALSGEEALRLFK
                     PYRPDLVLTDIRMPGMNGLELIQEVKQQETNLAIVVITGHGTLSTAIETMKEGVLGFV
                     IKPFTEDELLSAVKQALEKAALVKENMRLRSLMPLFEASKSLVSELNLDRLLDNVVAM
                     VKKETNADRVSIMLLDPVTKSLTVKAFVGFPTIIPQKLHRVIGNGIAGRVAQTGQPLF
                     IDRAQVQDTELADFLKDEDISSALSVPLISQGITTGVLNVAMLREGQLFTQSDLELVS
                     ILAGQAAAAVENAKLYQKTQLYYRKTIEALVTTLEIKDPYTKGHSIRVQKWACAIADV
                     MHLSPEDTETVRMSGLLHDIGKIGSSEEILLKEARLTPEEFERMKQHPRHSAKILGSA
                     GLSEEIIQSVLHHHEWYNGKGYPDGLSNKQIPLASRILLVADAVDAMSSDRPYRKALP
                     LEKLQTTLKEYSGTQFDPEIVEAVLQLLSQQGLKVVRSDGYENGLKT"
     gene            complement(1934..2983)
                     /locus_tag="VNV63_04650"
     CDS             complement(1934..2983)
                     /locus_tag="VNV63_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HXC61944.1"
                     /translation="MKAVVFHRYGGPEVLTYEEVLTPTIAENEVLIRVKACSINHVDI
                     WVRQGIPAYKISFPHISGCDIAGTVEEIGRDVFDRTDRQPGLAVGERVLVAPGLSCFR
                     CAYCLSGQDNMCLTYKILGAHTAGGYAEYVKVPAINVIPIPVNLSFEQAAAFPLVFLT
                     AWHMLITRAELKAGQDVLVLAAGSGIGNAGIQIAKLAGAWVIAAAGSDAKLAKAKELG
                     ADEVINYRREDFSTRTRDLTGGKGVDVVFEHVGPETWEKSLTALAKNGKLVTCGATSG
                     PEAKTDLRYVFTRQLSILGAMMGTRSELLEVAKLMEIGKLKPVIDSVYPLREARRAQE
                     YLLSRKNFGKLILVP"
     gene            3106..>3412
                     /locus_tag="VNV63_04655"
     CDS             3106..>3412
                     /locus_tag="VNV63_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013905160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXC61945.1"
                     /translation="MNDRDRAMMMGTMPAVEMKGVTKFYGNVRALDGLSLEVQEGEIF
                     GLLGPNGAGKTTTIKILITLLRPTEGEVKVLGCDAVHQAREVRQRIGYVPQERALDRF
                     "
BASE COUNT          830 a          900 c          916 g          766 t
ORIGIN      
        1 ctggccttga tccgatatta tgcggagaaa tccaatatct tgataaaaga agaatacgaa
       61 gaggttccca aggtgtcggt cgatgctaac cagatgaaac aggtattcat taatttgatc
      121 aaaaatgcga tggaggccat gccggaagga gggcatctta ggatcaagac ccgatggctt
      181 ctgaaagaac aacaggtgga aatcctcttc gaggacagtg gctccgggat tcaccaggaa
      241 catcttccaa agatctttga tccatttttc accacgaagg gcgagacggt cggaaccggc
      301 gtcggattag cggtcagcgc cggaatcatc aaacgtcacg gaggcgaagt gagcgtggaa
      361 agcacggtgg gacagggaag ccgtttctat gttcggctcc ctctagaagc ggagagagtt
      421 gaaaaggaag ccttgcgatg aaaaaccaac gaatcttggt cgtggatgac gaacctttta
      481 tcatagaact ctgtcagcgg ttcctgactg cggcaggcta tacggttcag accgcactca
      541 gcggcgaaga ggctctccgt ctcttcaaac cataccggcc ggatctcgtt ctaaccgaca
      601 tccgcatgcc gggcatgaac ggcctggagc tgatccagga ggttaagcag caagagacga
      661 atctggctat cgtggtcatc acgggacacg gaaccctttc gaccgccatc gagacaatga
      721 aggagggggt tctgggtttt gtgatcaagc cctttaccga ggatgagctt ttgtcggcgg
      781 tgaagcaggc cctggagaag gcggcccttg tgaaagaaaa tatgcgtctt cggtcactca
      841 tgccgctctt tgaggccagc aagtccttag tcagcgagct gaacctggat cgtcttttgg
      901 ataatgtggt cgcgatggtg aagaaggaga caaatgcaga ccgggtctcg atcatgctcc
      961 tcgatcctgt cacaaagtcc ttgaccgtta aggcctttgt cggttttcca acgatcatcc
     1021 cgcagaagtt gcatcgtgtt atcggaaacg ggattgccgg ccgagtcgcg cagacaggtc
     1081 aaccgctttt catagatcgt gctcaggttc aggatacgga gcttgcggat ttcctaaaag
     1141 atgaggacat tagctcggcg ctatcggttc cgctcatttc tcagggaatc accaccggcg
     1201 tcctgaatgt ggccatgctt cgtgaaggtc agcttttcac tcagagcgat ctggaactgg
     1261 tttctatctt agccggtcag gcggcggccg cggttgaaaa cgccaaattg tatcaaaaga
     1321 ctcagctata ttatcgaaaa accattgagg cccttgtcac gactttggag attaaagacc
     1381 cttatacaaa gggccactcg atccgcgtgc aaaaatgggc ctgcgccatc gccgatgtca
     1441 tgcatctctc accggaagac accgaaacgg tacggatgtc cgggcttctt catgacatcg
     1501 gaaagatcgg aagcagtgaa gagattttgt taaaggaggc caggctgaca cccgaagagt
     1561 ttgagcgaat gaagcaacac ccacgccact cggctaagat actggggtcc gccggacttt
     1621 cggaggaaat tatccagtct gtgctccatc atcacgaatg gtacaacggc aagggctatc
     1681 cggatggcct ttcgaacaag cagataccgc ttgcatctcg gattctgctg gtcgcggatg
     1741 cggtggacgc catgagctcc gatcgtccct accgcaaggc gcttcctctt gagaagcttc
     1801 agacgacact gaaagaatac agcggcactc aatttgatcc ggagatagtg gaggcggtct
     1861 tgcaactctt atcgcagcaa ggacttaagg tcgttcgatc agatgggtat gaaaacggtt
     1921 taaaaacctg accctacggg acgagaatca gcttaccgaa attcttccgg ctgaggagat
     1981 attcctgagc ccgtctcgcc tcccgcaatg gatacactga gtcaatcacc ggcttgagtt
     2041 ttccgatctc catgagtttg gcaacttcca acagctcgct tcgtgtcccc atcatggcac
     2101 caaggataga aagctggcgg gtaaagacat atcggagatc ggtcttggcc tcgggtccgg
     2161 aggtcgcccc gcaggtgacg agcttcccgt ttttggccaa tgccgtcagg ctcttctccc
     2221 aggtttccgg gcccacatgt tcaaacacca cgtccactcc cttgcctccg gtcaggtccc
     2281 gggtccgcgt ggaaaaatcc tctcggcgat aattgatcac ctcatccgcc ccaagctcct
     2341 tggccttggc cagtttggca tcactgccgg ctgctgcaat cacccaagca ccggcaagct
     2401 tggcgatctg tatccccgcg ttacctatcc cgcttcccgc cgccaagacc aggacgtctt
     2461 ggcccgcctt gagttctgcc cgtgtgatca gcatatgcca agcagttaag aagaccaacg
     2521 ggaaagccgc ggcctgctca aaagaaaggt tcaccggaat cgggatgaca ttgatggcgg
     2581 ggaccttcac atactccgca tatccgcccg cggtatgggc gcccagaatt ttataggtaa
     2641 gacacatatt gtcctgtccc gagaggcagt aagcgcaccg gaaacagctc agaccgggtg
     2701 ccacgaggac acgttctccc acggccaaac ccggctgccg atccgtccga tcaaagacat
     2761 cccgtccgat ctcttcgact gttccggcga tatcacagcc ggagatatgg ggaaacgaaa
     2821 tcttataggc cgggatcccc tgccggaccc aaatgtccac gtgattgatc gagcatgcct
     2881 tgacgcggat caggacttca ttctcagcaa ttgtaggcgt caggacctct tcatacgtga
     2941 gcacttcagg gccgccatac cgatgaaaga caaccgcctt catttttatc cgcctttttt
     3001 atctatcctg gtttaatcgg ggcttctaga gcgattttaa ttcttgcgca atgtcgtgta
     3061 attatagtat gttaatatcc cggtagcaag tgaattgcaa gtaacatgaa tgatcgcgat
     3121 cgagccatga tgatgggaac catgcccgcc gttgaaatga aaggtgtcac aaaattctat
     3181 gggaatgtcc gggccttgga tggcctctct ttggaagttc aggaaggtga aatctttggc
     3241 ctcctggggc cgaacggcgc aggcaaaacc acaacgatca agatcttgat cactctcctg
     3301 agaccaaccg agggcgaagt caaggtcctt ggttgtgatg ctgtccatca agcaagagag
     3361 gtgcgccaac gtattggtta tgtcccgcag gagcgcgcgc tggatcggtt tc
//
LOCUS       DATMKH010000386         2794 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_819933,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000386 DATMKH010000000
VERSION     DATMKH010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_819933"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..192
                     /locus_tag="VNV63_04660"
     tRNA            117..192
                     /locus_tag="VNV63_04660"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:150..152,aa:Arg,seq:ccg)
     gene            262..337
                     /locus_tag="VNV63_04665"
     tRNA            262..337
                     /locus_tag="VNV63_04665"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:295..297,aa:Lys,seq:ctt)
     gene            367..451
                     /locus_tag="VNV63_04670"
     tRNA            367..451
                     /locus_tag="VNV63_04670"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:401..403,aa:Leu,seq:tag)
     gene            477..1766
                     /gene="tig"
                     /locus_tag="VNV63_04675"
     CDS             477..1766
                     /gene="tig"
                     /locus_tag="VNV63_04675"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HXC61946.1"
                     /translation="MKVTVEEINPVKRALKIEVPMDVVSQAMSEAYADLKRRVKIPGF
                     RPGKAPLSLLEQRFAHDVTADVIRQLVPDYYQRAIKQVGLAPVEHPTIEKVELKKDNP
                     LSFVATVEIKPTIQVHDYLGLSVKRGKHSISEEDVEKTLEHLRDQMAQLVAYEDDHVI
                     ADQDFVLMDFIGYREGRPLEGGKAEGHQVQVGSKTLIPGFEEQLVGHRKGEEVKVEVT
                     FPTDYSQKALAGQPVTFQVVIREAKKKTLPALDDAFAQDVGAFKSLEELRVKLKQEML
                     DRLRREEDQIQKQEIMKQLIARQEFEVPVSLVQREMEVLLARVEQRQRQAGTATTPQA
                     IEKARQEYEPVARDRVKGWILLETIAGQEGIQVQEEEVQQAMERMATEMKMTAAQLKQ
                     LLLKREGSLEPFKGGLLQDKVLDFLLSKAVVEEGVAA"
     gene            1850..2449
                     /gene="clpP"
                     /locus_tag="VNV63_04680"
     CDS             1850..2449
                     /gene="clpP"
                     /locus_tag="VNV63_04680"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HXC61947.1"
                     /translation="MLVPIVIEQTSRGERAYDIYSRLLKDRIIFIGAPIEDNFANLII
                     AQLLFLESEDPEKDINLYVNSPGGLVTSGLAIYDTMQYIRPAVSTICTGQASSMGALL
                     LAAGAKGKRYALPNSRILIHQPMGGFQGQASDVDIHAREILRMRERLNEILAKHTGQP
                     LDKIRVDTERDYFMSGEEAQKYGLIDEVIAKAPAREKGK"
     gene            2476..>2794
                     /locus_tag="VNV63_04685"
     CDS             2476..>2794
                     /locus_tag="VNV63_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009370199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
                     /protein_id="HXC61948.1"
                     /translation="MARPEKSEAALRCSFCGKSREEVQKLIAGPTVYICNECIELCND
                     IIAEDWEEEKEQVTSKLPKPKEIKRILDQYVIGQERAKKVLSVAVHNHYKRISAAAAT
                     DEVE"
BASE COUNT          716 a          656 c          855 g          567 t
ORIGIN      
        1 agataggagt gctgactgcc gatatgatct tgacgatgga atcggtgcgg tgccctcttt
       61 agaaaaaggg ttgcatcctc aagacggtgt gcgatagaat gaataataat cggtgtgcgc
      121 ctgtagctca gtggatagag caacggcctc cggagccgtg ggtcgggggt tcaaatccct
      181 tcaggcgcgc caacactctt catgaaccca cgacccgcgg gtatcaaggg acgcgtcaag
      241 acgtgaagga gcctgttttt tgggccgtta gctcagttgg tagagcagct gactcttaat
      301 cagcgggtcg caggttcgat ccctgcacgg cccaccaaat gttttattga gcggcagggt
      361 ggaaaagcga gagtggcgga actggcagac gcgccagcct taggagctgg tcccgaaagg
      421 gttgggggtt caactccccc ctctcgcacc acggggaaat cttggatgga acttaaatga
      481 aggttacagt cgaagaaatt aatccggtca agcgggcctt gaaaatcgag gtgcctatgg
      541 atgttgtgag ccaggccatg agcgaagctt acgcggatct caagcgtcgg gtcaaaattc
      601 ccggtttccg gccaggcaag gcccctctga gcttgttgga gcaacggttc gcgcacgacg
      661 tgaccgcgga tgtgatacgg cagcttgttc cggactatta tcagcgggcc ataaaacagg
      721 tcggtcttgc gcctgtggag catcccacga tcgagaaggt agaacttaag aaagataacc
      781 cgctttcttt tgtggccacg gttgaaatca aacccaccat tcaggtgcat gattatctgg
      841 gcctttctgt gaagcgaggg aaacattcca tcagtgagga agacgtggag aagacgttag
      901 aacaccttcg agatcagatg gcgcagctcg tggcttatga agatgatcat gttatcgcgg
      961 atcaggattt tgtgttgatg gattttatcg gctatcgaga ggggaggccc ctggaggggg
     1021 gcaaggcaga gggccaccag gttcaggtcg gttccaaaac attgattccc ggctttgaag
     1081 aacaattagt gggacaccgg aaaggggagg aggtgaaggt cgaagtgacc ttcccgacgg
     1141 attattctca aaaagccctg gccggacaac cggtcacttt ccaggtggtg atccgggagg
     1201 cgaagaagaa gacgctgccc gctttggacg acgcattcgc ccaggatgtg ggtgcgttta
     1261 agtctctcga ggagttgaga gtcaagctca aacaggagat gctggaccgg cttcgccggg
     1321 aagaggacca gatccagaag caggaaatca tgaagcaact cattgcccgt caggaatttg
     1381 aggttccggt ttccttggtt cagcgggaga tggaggttct gttggcacgc gtagagcaac
     1441 gacaacgaca ggccggaacc gctacaactc cgcaggccat cgagaaggca cgtcaagagt
     1501 atgagcccgt ggccagagat cgtgtcaaag gctggattct tctggaaacc attgcaggtc
     1561 aggaggggat acaggtccaa gaggaagagg ttcagcaggc catggagcgt atggcgactg
     1621 agatgaaaat gaccgcagca cagttgaaac agttacttct gaaacgggag ggcagtctcg
     1681 aaccgtttaa gggaggtctg ttacaagaca aggtgctgga ttttttgctc tcaaaggcgg
     1741 tggtggaaga gggtgtggcg gcataaaaga gataagagat gatcgggcct gaaagatttc
     1801 tcgcccgtta aacagaattc atttgcccga gttgaaaagg gaggaaacga tgttagtacc
     1861 gatcgtgatc gagcagacca gtcgcggaga acgcgcttat gatatctact cgcgcctgct
     1921 caaagaccgc atcatcttta tcggcgcgcc catcgaggac aacttcgcga atctcattat
     1981 cgcccagctg ctcttcttgg aatccgagga ccctgaaaaa gatatcaatc tctacgtcaa
     2041 cagcccgggc ggtctggtca cctccggact cgccatctat gatacgatgc aatatatccg
     2101 gccggcggtt tccacaatct gtaccgggca ggcctccagc atgggggcat tgctccttgc
     2161 agccggggcg aaagggaaac gctatgccct accaaactcc cgcatactga tccatcagcc
     2221 gatgggcgga tttcaggggc aagcctccga tgtggacatt catgccagag agatcttgag
     2281 aatgcgggag cggctcaacg agattctggc caaacatacg gggcagccgc tggataaaat
     2341 ccgtgtggac accgaacggg actatttcat gtccggagaa gaggcccaga agtacggact
     2401 gattgatgaa gtgatcgcaa aggcacccgc acgcgaaaaa gggaaataat atagcggggg
     2461 aaatggagaa atttgatggc gcgtccagag aagagtgagg ctgcattgcg ttgctcattc
     2521 tgcggaaaga gtcgggaaga ggttcagaag ctgatcgcag gcccgaccgt ttacatctgc
     2581 aatgaatgta ttgaactctg caatgacatc atcgcggaag actgggagga agagaaggag
     2641 caggttacct cgaaacttcc gaagccgaag gagatcaagc gcatcctgga ccagtatgtc
     2701 atcggccaag agcgtgccaa gaaggttctc tcggtggcgg tccacaatca ctacaagcgg
     2761 atcagcgcgg ctgcggcgac cgatgaggtc gagc
//
LOCUS       DATMKH010000387         1139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_820557,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000387 DATMKH010000000
VERSION     DATMKH010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_820557"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            348..>1139
                     /locus_tag="VNV63_04690"
     CDS             348..>1139
                     /locus_tag="VNV63_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61949.1"
                     /translation="MGKLPAFMFYPGDWQKDPCLRRCSKAAKGVWMDMLCLLFECPVR
                     GVFVDASGKPWSDEEIAEAIGGDIGANMGHIAELVSKGVAQRDTRGAIFSRRMVRDEQ
                     NRRSATERKRKERSSHTSVTPMSVNESEIGRQSKLFVEVKGFEEEKKMIDTLSSVVDQ
                     IGHLHPANSHLHGRSLPQGQQDAIAEAVAKDGADQVLSGTRNFADKVAIWPPSDLRFI
                     PNPAKFYSEGQYLKRPEFWERRKENGREECTLHPDSGLTQWGTCWA"
BASE COUNT          304 a          254 c          333 g          248 t
ORIGIN      
        1 gatccatcct cggcatcggc ctctgagaac agaggtgcga gcatttcttc agatggtcag
       61 ggaggcggcg aatgaggatt ctagtgccgg caacgcaacg aggaagaagc ggaagcacca
      121 gagataatcg agctaaactc acgccgagca ggatttgact agaatcgact ctagccatct
      181 tttccgccct cgtccacaac ctggaccaca tctccacagc atgtgcacag cctgagagtt
      241 ttttcctgtt gcgtgaaaga ggacaatcaa tcactcttca aaacgcaccc tcaatcatga
      301 cctttctatc cgtgagtgtt ctggcggaat catagaggaa tgtaagaatg ggaaagttac
      361 cggctttcat gttttacccg ggggattggc agaaagaccc ttgccttcgg cggtgctcta
      421 aggctgcgaa gggtgtatgg atggatatgc tctgtttgct tttcgaatgt ccagttcgcg
      481 gcgtgttcgt cgatgccagc ggtaagcctt ggagcgatga agaaatcgca gaggccatcg
      541 gtggcgacat cggcgcgaac atgggacaca tcgcggagtt agtgtcgaag ggagtggctc
      601 agagggatac tcgtggagcc attttctcgc gacgcatggt tcgggatgag cagaatcgtc
      661 gatcagctac agagagaaaa cgaaaagaac gttccagtca cacgagtgtc acgccaatgt
      721 cagtaaatga aagtgaaatt ggaagacaaa gtaagttgtt tgtagaagta aaagggtttg
      781 aagaagaaaa gaaaatgatc gacactctaa gttcagtagt cgatcagatt ggacatctcc
      841 atccggcaaa cagccacctg catggccgat ccctgccaca gggacagcaa gatgccattg
      901 cggaagcggt ggcgaaggat ggagccgatc aggttctttc tggaaccagg aattttgcgg
      961 acaaagtggc gatttggccg ccgtcggatt tgcggtttat tcctaacccg gctaagttct
     1021 acagcgaggg ccagtatttg aagaggccgg agttttggga gaggagaaaa gaaaatggac
     1081 gagaagagtg tacgctgcat ccagactctg gtctcaccca gtgggggacc tgttgggca
//
LOCUS       DATMKH010000388         2051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_181672,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000388 DATMKH010000000
VERSION     DATMKH010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181672"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="VNV63_04695"
     CDS             <1..287
                     /locus_tag="VNV63_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61950.1"
                     /translation="MGMRIQLQQKMLEKWIGKLDALSPLSILARGYSIARRLPLMTFI
                     KDAGEMQRGDLFHLTLHRGALISRVEEVRAPAGNDIDGPLREIRRAPLGD"
     gene            295..519
                     /gene="xseB"
                     /locus_tag="VNV63_04700"
                     /pseudo
     CDS             295..519
                     /gene="xseB"
                     /locus_tag="VNV63_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511907.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
     gene            611..1435
                     /locus_tag="VNV63_04705"
     CDS             611..1435
                     /locus_tag="VNV63_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HXC61951.1"
                     /translation="MSSTLRAAKVKAPRERLDKLLVARGYAQSRERAHAMILAGQVWV
                     EGRPSAKAGLCLPLDVEIELHGSVLQYASRGGLKLEHALAAFSIQPVGWVAMDAGAST
                     GGFTDCLLQHGVKKVYAVDVGYGQLAWRLRQDPRVVVIERTNIRYMTEKTVPEKVDLI
                     TVDLSFISLRLVLPKLLAFLKSTGPFGKYDDPSGKCNSRIVALVKPQFEVGKGQVGRG
                     GLVKDTAKHQEVLEMISQAAAKTGLTVEGITASPLLGRKGNQEYFICLKTGPLREI"
     gene            1488..>2051
                     /locus_tag="VNV63_04710"
     CDS             1488..>2051
                     /locus_tag="VNV63_04710"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HXC61952.1"
                     /translation="MSVLVTGGAGFIGSHVVERLLNQDKEVICLDNFDPFYDPMMKRQ
                     NLARVGPHPRFHLVEGDIRDQRLLEKLFSDYPITHVFHAAAKAGVRPSIQDPLGYEAV
                     NIQGTIGLLEMARKRTIENFVFASSSSVYGNSPHLPFTENQPLGIACSPYGATKQAGE
                     LFCATYHLLYQLPVIGLRFFTVYGPRQR"
BASE COUNT          498 a          516 c          585 g          452 t
ORIGIN      
        1 aaatgggaat gcggattcaa cttcaacaga aaatgctcga gaaatggatc ggaaaactcg
       61 atgccttaag ccccctctcg attttggccc gaggatacag cattgcgcgt cggttgcctc
      121 tcatgacgtt tatcaaagat gccggggaga tgcagcgcgg cgaccttttc cacctcacgc
      181 tccaccgcgg cgcgcttatc tcgcgtgtcg aagaggtgcg ggcccccgcg ggaaatgata
      241 tagacgggcc cctccgggag attagacggg cccctctggg agattaaaat aaagatggcc
      301 aatttaaaat tcgaagatgc catggctcgc ttggaagaga ttgtcaatgg tttagagaaa
      361 ggcgacctcc ctcttgatga atccttgacg atgttcgagg aaggagtccg gctctcaaaa
      421 agctgcctta agatgctgaa tgaggcgcaa aagaaggtcg aggtcctggt tcgcgataaa
      481 gaagggaaga aaaaagttcg acctttccga ctggaagaga cgggggcccc tcaaaaagat
      541 acgacgatcc ctccggaaga tatggggacc cctctgggag acatgatgtt ccctccggaa
      601 gacgaagaag atgagtagta cgctacgggc cgccaaggta aaagcaccac gagaacgact
      661 ggacaagctc cttgtcgcac gggggtatgc ccaaagccgt gagcgggccc atgccatgat
      721 tctagccggc caggtctggg tggaggggcg gccgtctgca aaggccggat tgtgcctgcc
      781 attggatgtc gagatcgaac tgcacggatc cgtcctgcaa tatgcaagca ggggtggact
      841 gaagctcgaa catgccttgg ccgctttttc gatccaaccg gttggatggg ttgccatgga
      901 tgcgggcgct tctaccggtg gatttacgga ctgccttctc caacacggcg tgaaaaaggt
      961 gtatgcagta gatgtcggtt atggacaact ggcctggagg cttcggcagg accctcgtgt
     1021 agtcgtaatc gaacgcacca acatccgata tatgaccgaa aaaactgttc cggaaaaggt
     1081 agaccttatc actgtggatc tctccttcat ctctttgagg ctggttttgc cgaagctctt
     1141 agcgtttctg aaatccacgg gcccctttgg gaaatacgat gatccctccg ggaagtgtaa
     1201 tagccggatc gtagccctgg tcaagccaca gttcgaggtc ggaaaaggtc aggtcgggcg
     1261 cggcggtctt gtcaaagaca ccgcaaagca ccaggaggtg ctggagatga tcagccaggc
     1321 agcggctaaa actggtctga ccgtagaggg aattaccgcc tctcctctac ttgggcggaa
     1381 ggggaatcaa gaatatttta tttgtcttaa aacagggccc ctccgggaga tatgatgggc
     1441 ctccgggaag atataatagc ccctccggga gatataaagg tgcctgcatg agcgtgctgg
     1501 tgaccggggg agccggattc atcggatccc atgtcgtgga gagactccta aatcaggata
     1561 aagaggtgat ctgtctcgat aacttcgatc cgttttatga cccgatgatg aaacgacaga
     1621 atcttgcgcg ggttggacca catcctcggt ttcatcttgt tgagggagac atccgagacc
     1681 agcgcctctt ggaaaaactc tttagcgact atccgatcac ccatgtcttc catgcagccg
     1741 ccaaggccgg tgtcaggcct tcgattcaag accctttagg atatgaggct gtaaatatac
     1801 aggggacgat cgggctttta gagatggccc gaaagcggac gatagagaac ttcgtgtttg
     1861 cctcttcctc ttcggtttat ggtaacagcc cacacctccc tttcaccgaa aatcagccgc
     1921 ttggaattgc ctgttctccg tacggggcaa ccaagcaggc cggagaactg ttttgcgcca
     1981 cgtatcacct tctctaccag ttgccggtaa tcggcctacg tttctttacg gtgtatggtc
     2041 ccagacagcg c
//
LOCUS       DATMKH010000389         4797 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4015807, whole genome shotgun sequence.
ACCESSION   DATMKH010000389 DATMKH010000000
VERSION     DATMKH010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4797
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4015807"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..2474)
                     /locus_tag="VNV63_04715"
     CDS             complement(168..2474)
                     /locus_tag="VNV63_04715"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014792249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HXC61953.1"
                     /translation="MELTQTATRLKGQWTANAIRVMEERYLKKEHGKVRETPDEMFSR
                     VARAVAKAELKWGKTEIETEEIAGQFYEMMVRCRFMPNSPTLMNAGKENGLQFSACFV
                     LPVSDSIRGIFDAIKNAAIIHQSGGGTGFSFSRLRSKGSIVSTTQGEASGPVSFLKVF
                     DAATNSIKQGGTRRGANMGILQCDHPDIMEFIFCKRQRQITNFNISVAITDSFMEKVQ
                     RGEDYDLIAPHTGKPVGRLNARKVFDEIVESAWQSGDPGLIFMERVNQGPSNPVPKMG
                     PVEATNPCGEQPLYPNEACNLGSLNLMAFAVQSQGVWDLDWVEMEKAVRLSVRFLDDV
                     IDVNPYPLLEIDEAVKSNRRIGLGVMGWAHLLFRLGISYESEEALSLGRRVMQFINQI
                     GHEESERLAEVRGPFPNWADSIYKDGKPLRNSTVTTIAPTGTISIIAGCSSGIEPIFA
                     LAFQHIVGDRKLDFVDPVFEAIAKEKGFYSPALREEVMKAGSLAHLPEISEEAQRVFR
                     ASHEIPPDWHIRTQGAFQEFTDNAVSKTINLPNSATQADVEHAYLMAYQLKCLGITVF
                     RDGCLDQQVLNIGVGSEATKETPKVAKVEESPKVKPRPYKRVGATMSKATPFGTAHIT
                     MNDDEEGPAEVFITIGKAGSDVMAMAEGYGRSISLYLRTESILSRVEKVKEYVSQLRG
                     IGGSRSVGFGDARVASLPDAIARAMAEHWLIDVQTPVLSSGDGLVNENGGNGSPKLTG
                     NLCPECGGMTLAREEGCQKCHSCGFSEC"
     gene            complement(2880..3011)
                     /locus_tag="VNV63_04720"
     CDS             complement(2880..3011)
                     /locus_tag="VNV63_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61954.1"
                     /translation="MIVAGISILFIRLNLTEQEKRDLVEFLKALTGEKLNIAMPKLP"
     gene            3075..3398
                     /locus_tag="VNV63_04725"
     CDS             3075..3398
                     /locus_tag="VNV63_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012501218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HXC61955.1"
                     /translation="MITLTENAIKEVRRLMQQQQKPDLALRLNVKGGGCAGMEYLLNF
                     DTEKKEWDEQFQIDGIRVLVDAKSMLYLEGITVDYSYDLVNGGFRFANPNAKSSCGCG
                     TSFSV"
     gene            3480..4205
                     /gene="hscB"
                     /locus_tag="VNV63_04730"
     CDS             3480..4205
                     /gene="hscB"
                     /locus_tag="VNV63_04730"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HXC61956.1"
                     /translation="MSMGNKTPQNHLTAEENVCWACHHPRGTGHLCRYCSVLQPLLNS
                     ENRFSVMGLSPRLNLDFSSLQRTFYELSRNYHPDFYQTRTSEEKSISEAATAMINTAY
                     ETLNDPIRRVEYLLTLEGMPAERGESKPPMDLFEEILSMQETLSDYREAKLNKTANLD
                     LAQAAVLKTNEDLESRQKGLAKRLEALSLRWDALLDSSSTIHKPDSEEKKKILTELRD
                     TLGEIAYLRTVLRDIQKTIETRS"
     gene            4208..>4797
                     /locus_tag="VNV63_04735"
     CDS             4208..>4797
                     /locus_tag="VNV63_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HXC61957.1"
                     /translation="MGRVIGIDLGTTNSLAAYMEGGQPKIIPDLDGHRLVPSMVTFTP
                     EGVVVGEQAKQLRAENVGNTIYSVKRLMGKGIGEVKAELTMVPFQVSDQSNEVIRITI
                     NGHDYTPPELSALVLKQLKTQAEKFFGEPVNQAVITVPAYFNDSQRQATKDAGQIAGF
                     EVLRILNEPTAAALAYGLDKKRQGIIAVYDLGGGTF"
BASE COUNT         1201 a         1271 c         1206 g         1119 t
ORIGIN      
        1 tatcatgatt tcccgcaaaa gagtttacac cgaatcggac attctggcaa ggagttgttg
       61 agggcgtttg ggactggagt ttcgtataac aagctaaagc cgtagggaca attcttaaac
      121 ataaaaacga attgtcccta cggtaagtga gagttttcag taaccaccta gcattcgctg
      181 aacccgcaag agtggcattt ctgacagccc tcttcacggg caagggtcat accgccacat
      241 tccgggcata aatttccggt gagctttggc gaaccattac ctccattttc gttaaccagt
      301 ccatcgcctg atgacagaac cggtgtctgt acatcaatca accaatgttc tgccattgcg
      361 cgcgcgatcg catccggaag agaagccaca cgagcgtcac caaaaccgac agagcgagag
      421 ccaccgattc ctcgaagttg gctcacgtat tcctttactt tttcaaccct ggagaggata
      481 gactctgtcc taagataaag tgatatcgac cggccgtacc cttcggccat cgccatgaca
      541 tcactccccg ccttgccgat cgtgatgaac acctcggcag gtccctcctc gtcatcgttc
      601 attgtgatgt gggcggttcc aaaaggtgtg gctttactca tcgtcgctcc gacccgctta
      661 tatggccggg gtttcacttt cggagactct tccactttag ctactttcgg ggtctccttg
      721 gtcgcctcgc ttcccacgcc gatattcaac acctgctgat cgaggcagcc gtcacggaac
      781 accgtgatcc caaggcattt aagttgatag gccatgagat aggcatgctc gacatcggcc
      841 tgtgtcgccg agttcggcag attgatcgtc ttagaaaccg cattgtcggt aaattcctgg
      901 aaggcccctt gggtccgaat gtgccaatcc ggagggatct catgagaagc ccggaagacc
      961 cgttgggcct cttctgaaat ctcgggtaaa tgggccaatg atccggcttt catgacctcc
     1021 tccctcaggg ctggactgta gaaccccttt tcttttgcga ttgcctcaaa aaccgggtcc
     1081 acaaaatcca gcttgcggtc gcccacgata tgctgaaagg caagcgcaaa aatcggctcg
     1141 atcccggaag agcagccggc gatgatgcta atcgtcccgg tcggcgcgat cgtggtcacg
     1201 gtggagttgc gaagcggttt tccatccttg tagatgctgt cggcccagtt gggaaacgga
     1261 ccgcgtacct ccgccaaccg ctccgattct tcatggccga tctggttaat aaactgcatg
     1321 acgcggcgtc ccagagacag agcctcttcg ctctcataag aaatcccgag acggaaaaga
     1381 aggtgcgccc aacccatgac acccaggccg atccttcggt tggatttcac tgcctcatca
     1441 atctccagca gcggataagg gttcacatca atcacatcat ccaggaaccg cacactgaga
     1501 cgaaccgctt tctccatctc gacccagtca agatcccaga caccttgact ctgtacagcg
     1561 aaggccatca gattgagcga gcccaagttg caggcctcgt tgggatagag cggctgctct
     1621 ccacaaggat tcgtcgcctc gaccggaccc attttaggca ccgggttaga cggtccctga
     1681 ttgacccgct ccataaagat caggccagga tcaccgctct gccaggccga ttccacgatc
     1741 tcatcaaaca ccttccgggc attgagccga ccgactggct taccggtgtg cggcgcaatg
     1801 aggtcataat cctctccccg ttgcaccttt tccataaagc tgtccgttat ggctacagag
     1861 atattgaagt tggtaatctg tcgctggcgt ttgcagaaaa taaactccat gatatcagga
     1921 tgatcacact ggaggatgcc catgttggca ccgcggcgtg tccccccctg tttgatcgaa
     1981 ttggtcgctg cgtcaaagac ctttaaaaaa ctcaccggcc cgctggcctc tccctgcgtg
     2041 gtggagacga ttgatccctt ggaacggagg cgcgagaaag aaaatccggt acccccaccg
     2101 gactgatgga tgattgcggc attcttgatc gcatcaaaaa taccccggat cgagtcgctc
     2161 acaggaagga cgaaacaggc tgagaactgt aacccgtttt cttttcccgc attcatgagg
     2221 gttgggctgt ttggcataaa acggcatcgg accatcatct catagaactg accagcgatt
     2281 tcttccgtct cgatttcggt cttgccccat ttgagctccg ccttggccac ggcacgggcc
     2341 acacggctaa acatctcatc cggcgtctcg cgaacctttc catgctcttt tttgagataa
     2401 cgttcctcca tgacacggat ggcattggcg gtccattgac ccttcaagcg agttgccgtt
     2461 tgagtaagtt ccattttaat tttacctcct agttaaagat gaaactaaac catctttcta
     2521 tctgcatcta aagatgtgct tcagattcat tggaaccccc tattgggctt ttgatgacaa
     2581 tctccgtgca gtagtaaaat tcggtgtgag aattgtcgta ctatataaaa actgaaacga
     2641 gttgtcaagg aaaaaataca acatatagaa tagcaggctt cagttgactg ctatatattg
     2701 tggattactt ggggataagg atgtgaatag aaactcaata catttggctt ttctatcggt
     2761 ggcaattaga aataaagcgg cctccccatg aggccgccct aaagtcagaa aaatgtcttg
     2821 accttattta gagatgtcca tatatctccc ggaggggccc gagatcttct tagatccggt
     2881 taaggcagtt taggcatcgc gatgtttaac ttctcacccg tgagggcctt caggaactcc
     2941 accaggtccc tcttctcctg ctctgtgagg ttaagccgga taaaaaggat tgagatgccg
     3001 gcaacgatca acagttgtta ttcgcccggg gcctgtgcta taatgagtct ggtttgaata
     3061 ggaggtaaaa tgccatgatc accctaacag aaaatgcgat caaagaggtt cggcgtctga
     3121 tgcaacagca gcagaaaccg gatctcgccc ttaggttgaa cgtaaaaggt ggcgggtgcg
     3181 caggcatgga gtatttgcta aacttcgaca ctgaaaaaaa ggagtgggac gagcagttcc
     3241 agattgacgg cattcgagtg ctggtggacg caaaaagcat gctgtatctg gaggggatta
     3301 ccgtggatta ttcgtatgac ctggtcaatg gcgggttcag gtttgccaac cccaatgcca
     3361 agagcagctg tggatgcggc acctcgtttt cggtctaaga agttaagacc tgaccgaaag
     3421 cagtgtccta aacaagcggg atatccccgc tttatttttt agttgtccaa gctgtacaaa
     3481 tgtccatggg aaacaagacc cctcaaaacc atttaaccgc tgaggaaaac gtctgctggg
     3541 cgtgtcatca tcctagagga acaggccatc tctgccggta ctgctccgta cttcaaccgt
     3601 tgctcaactc cgaaaaccgc ttttcagtaa tgggcctttc gccgcgactg aatttagatt
     3661 tttcttcctt acagcggaca ttctacgaac tgagccgcaa ctatcacccg gatttttatc
     3721 aaacccgaac atcggaggag aaatccatca gcgaagccgc gaccgccatg atcaatacgg
     3781 cgtatgaaac tttgaacgat ccaataaggc gcgtcgaata tctcctcacc ctggaaggga
     3841 tgccggcgga acgaggggaa tcgaaaccac cgatggatct ctttgaggag atcttgagca
     3901 tgcaggagac actcagcgat tatcgggagg ccaagctcaa caaaacagct aatctggact
     3961 tggcccaagc cgcggttctc aaaaccaatg aagatttgga atctcgtcag aaagggttgg
     4021 caaaacgact tgaggcgctc tcgctccgtt gggacgcttt gttggattcc tcttcaacca
     4081 tccataaacc tgattcggaa gagaaaaaaa agatcctgac agagttgcgg gacacactgg
     4141 gcgagatcgc ctacctgcgt acggtcttgc gggacattca aaagacaatc gaaacaagga
     4201 gctaattgtg ggaagagtta ttggtattga ccttggaacg accaacagtt tggcggctta
     4261 catggaaggc ggccagccca agatcatccc ggacctggac ggccatcgcc tggtcccttc
     4321 aatggtcact ttcacaccgg agggcgttgt ggtgggcgaa caggccaaac agctccgtgc
     4381 cgagaatgtc ggaaatacga tctactcggt caagcggctg atgggcaaag gaattgggga
     4441 ggtcaaggca gaactcacca tggtgccttt tcaggtatcg gatcagagca acgaggtaat
     4501 tcgtatcacg atcaacgggc atgattatac gcctccggag ctttccgcgc tggtgttaaa
     4561 acagctcaag acccaggccg aaaaattctt cggcgagccg gtaaatcagg ccgtgatcac
     4621 cgttccggcc tacttcaacg acagccaacg ccaagccacc aaggacgccg gccagattgc
     4681 cgggttcgag gttctgcgga ttttaaacga gccgaccgca gcagccttgg cctacggctt
     4741 agataaaaaa cgtcagggca ttatcgcagt ctacgatctg ggcggcggga cattcga
//
LOCUS       DATMKH010000390         1039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4659324, whole genome shotgun sequence.
ACCESSION   DATMKH010000390 DATMKH010000000
VERSION     DATMKH010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4659324"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="VNV63_04740"
     CDS             complement(<1..612)
                     /locus_tag="VNV63_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013568718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HXC61958.1"
                     /translation="MAVMMQAFYWDAPIKENKRGEWWNFVGEKVPQLAASGINSLWLP
                     PFSKAADPDSNGYDPYDYFDLGDFDQKGSVKTLYGNRAELEQLIKLMREHNMSGIADM
                     VINHNSGADEEEMNPLDGKKRWTKYIPKSGRFPRDWNCFHPSRYERTMIEGEKFAGFP
                     HLCHRNPRVYEAMFEYSRMAIEELDFDGLRFDFVKGFGAWMIGL"
     gene            complement(612..848)
                     /locus_tag="VNV63_04745"
     CDS             complement(612..848)
                     /locus_tag="VNV63_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61959.1"
                     /translation="MKVEELIGSKVWIKRDRFRSVSGKIEGMSNVVNLDDIQSSVSQS
                     TFRIEMPSGNIIEILGSDISKIEMPVTSEEGLPA"
BASE COUNT          232 a          304 c          234 g          269 t
ORIGIN      
        1 cagtccgatc atccacgctc caaagccctt cacaaagtcg aaccggaggc cgtcgaaatc
       61 aagttcctcg atggccatac gcgaatactc gaacatcgcc tcatacacac gaggattgcg
      121 atgacacaag tgcgggaatc ccgcgaactt ctcgccctcg atcatcgtcc gttcgtatcg
      181 gctcggatgg aagcagttcc agtcgcgcgg gaaccttccg ctcttcggaa tgtacttcgt
      241 ccagcgcttc ttgccgtcca acgggttcat ctcttcttcg tccgcacctg agttgtggtt
      301 gatcaccata tcggcgatcc cgctcatgtt gtgctctcgc atcagcttga tcagttgctc
      361 aagctcggcg cgattgccat acaaagtctt cactgaacct ttttgatcga agtcaccaag
      421 atcgaaatag tcgtacgggt catagccatt tgagtccggg tctgcggcct tgctgaaagg
      481 tggcagccac aaggaattga ttcccgacgc cgcgagttgc ggcacctttt ccccaacgaa
      541 gttccaccac tccccacgct tgttttcttt gatcggagca tcccaataaa acgcctgcat
      601 catgacggcc attatgccgg aaggccttcc tcggatgtaa ccggcatttc gatctttgaa
      661 atatcggaac cgaggatctc aatgatgttt ccagaaggca tctcgatcct aaacgtactt
      721 tgagagacgg atgattggat gtcgtctagg ttgacgacat tagacatacc ttcgatcttg
      781 cctgacacac tccggaatcg gtctcgcttg atccacacct tgcttccgat caactcctca
      841 actttcatgt cagtctccag tttgaaggtg tcctgcgcca tccagcctta gcgtatctac
      901 agcatgttag tcagacaccg ttcaactttg ccacttcgca atcttcagga aaaatgaggc
      961 tcttagcaac gtggctaaga gccttgatgg gagcgtatgt ctcctatctc tcttatcggc
     1021 cattgctcca cgttaagca
//
LOCUS       DATMKH010000391         1217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1777973, whole genome shotgun sequence.
ACCESSION   DATMKH010000391 DATMKH010000000
VERSION     DATMKH010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1217
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1777973"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1216)
                     /locus_tag="VNV63_04750"
     CDS             complement(<1..1216)
                     /locus_tag="VNV63_04750"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HXC61960.1"
                     /translation="MGGGDSAIEIALSLAEQNRVSMAVRTSEFIRVKDSLERQALEKA
                     KKGQLNIYFNSSVERIEPEMMKLKLSQSTIDVKATVVIAKIGTVPPRSFLEKCGIAFP
                     SQDREATPLLSASYETNVPGLFLIGAVGGKELIKHGINQGYEVVEHLCGRPVEPADEA
                     LLRQKLGITAGTVSGRIQELLPMTPVLSGGTEAQIRELLLFSQFHRVQPGEIVFRQND
                     FSESLYMILEGRVELLVKPDDGPEKSVATLGPGEFLGEMSLISGRRRSATVRAVSEGL
                     LWEVTRKAMLKFIYTTPAAKKFIDQIFLIHAFQSYLFPNLDHDILVRLASQAEVLKFD
                     KGKIIIKEGDPGDAFYLLRSGMVKVSKMREDQEIVLAYLAAGQYFGEMALLNNAPRAA
                     TVAAIDKVEVIKL"
BASE COUNT          277 a          344 c          293 g          303 t
ORIGIN      
        1 agagcttaat aacctccacc ttgtcaatgg cagcaacggt agctgcacgg ggcgcgttgt
       61 ttaaaagcgc catttcgcca aagtactggc cggccgctaa gtaggccagg acgatttcct
      121 ggtcttcccg catcttgctg accttgacca tgcctgaacg gagaagataa aatgcatcac
      181 ccggatcgcc ctccttgatg ataatcttcc ccttatcaaa cttgagaacc tcggcttggc
      241 tggccagccg gaccaggatg tcatgatcga gatttggaaa aagatagctc tggaaggcat
      301 ggatgagaaa gatctgatcg ataaatttct tggcggccgg agtggtgtag ataaacttga
      361 gcatggcctt tctggtcact tcccagagaa gcccttcgct caccgccctg accgttgcag
      421 agcgacgccg gcctgagatc aggctcatct ctcccaagaa ctcgcccggg cccagggttg
      481 ctacggattt ctccgggccg tcatccggtt ttacgagcaa ctccacccgg ccctccagaa
      541 tcatgtacaa gctctccgaa aaatcgttct gacggaacac gatttctccc ggctggactc
      601 tatgaaattg cgagaaaaga agcagctctc gaatttgtgc ttccgtcccg ccggacagaa
      661 caggagtcat tggaagcagc tcctggatgc gccctgacac tgttcctgct gtgatgccaa
      721 gcttctgacg aagcaaggcc tcatcagccg gttccacggg tcttccgcag aggtgctcca
      781 ccacttcata tccttggttg attccatgct taatcaattc ttttccgccc accgcaccga
      841 tgaggaaaag tcccggaaca tttgtctcgt aggaggcgct tagcagcgga gttgcttctc
      901 gatcctggga gggaaaggcg ataccgcatt tttctaaaaa gcttcttgga ggaacggtgc
      961 cgatcttagc aattaccacc gtggctttta catcgatggt cgattgggaa agtttcaatt
     1021 tcatcatttc cggctcaatc cgttcaacgc ttgaattaaa gtaaatgttc aattgtccct
     1081 ttttggcctt ctccagggcc tggcgttcca agctatcctt caccctgata aactcagatg
     1141 ttcttacggc catagaaacc cgattttgtt ctgccagtga tagcgcgatc tcgatcgccg
     1201 agtctccccc ccccact
//
LOCUS       DATMKH010000392         1125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4659461, whole genome shotgun sequence.
ACCESSION   DATMKH010000392 DATMKH010000000
VERSION     DATMKH010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4659461"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1009)
                     /locus_tag="VNV63_04755"
     CDS             complement(<1..1009)
                     /locus_tag="VNV63_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020494395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HXC61961.1"
                     /translation="MQIQTPEVQTHELVKPPGPLWFKEAIVYQLHIKTFCDANSDGIG
                     DFQGLLSKLDYLKNLGVTAIWLLPFYPSPLKDDGYDIADYFTVNPSYGTLDDFKQFLD
                     AAHQIGLRVITELVLNHTSDQHIWFQKSRRAAPGDPWRDYYVWSDDPTKYDEARIIFK
                     DYEVSNWTYDPIAKSNFWHRFFSHQPDLNYDNPEVKAAASKVLDFWLDLGVDGLRLDA
                     VPYLIEREGTSCENLPETHRILQELRARIDAKYTDRMLLAEANQWPEDAVAYFGSGNE
                     CHMCFNFPLMPRMFMSLHQEDRYPIVDILEQTPAIPENCQWAIFLRNHDELTLEMVTE
                     EE"
BASE COUNT          265 a          266 c          313 g          281 t
ORIGIN      
        1 gctcttcttc tgtcaccatc tcgagtgtca gttcgtcatg gttccgaagg aagatggccc
       61 attgacagtt ctctggaatc gcgggtgttt gttcgaggat atcgacgatc ggataacggt
      121 cctcctgatg cagcgacata aacatccgcg gcatcagggg gaaattgaaa cacatgtggc
      181 attcgttccc tgaaccgaaa tacgcgacgg catcctccgg ccattgattg gcctccgcca
      241 aaagcatgcg gtccgtgtat ttggcgtcga tgcgggcccg taattcctgc aaaatccgat
      301 gggtttccgg caagttttcg cagcttgttc cctcccgttc aatcaggtag ggaaccgcat
      361 cgaggcggag tccatctaca ccaagatcga gccagaaatc gagcaccttg gaagcggcgg
      421 ctttcacctc aggattgtcg tagttaaggt cgggctggtg agagaagaag cgatgccaaa
      481 aattggactt ggcaatcgga tcataggtcc aattgctcac ttcataatct ttgaagataa
      541 tgcgggcttc atcgtatttg gtgggatcat cggaccatac gtagtaatcc cgccagggat
      601 ctccaggcgc tgcgcggcgc gatttttgaa accagatatg ctggtcgctg gtgtgattca
      661 gcaccagttc agtgatcacc cgcaacccga tctgatgagc agcatccagg aattgcttga
      721 aatcatccaa ggtgccatag gatggattta cagtgaaata gtcggcaata tcatatccat
      781 cgtccttgag gggcgatggg tagaacggca aaagccagat cgcggtcacg ccaagatttt
      841 tgaggtaatc cagtttgctc agtaatccct gaaaatcacc gatgccgtca ctattcgcat
      901 cgcagaaggt cttaatatgg agctgataaa cgatggcttc cttgaaccag agcggtcctg
      961 gcggctttac gagttcgtgg gtttggacct caggcgtttg aatctgcata ggggaagtac
     1021 gttagcgttt agcgtttggc gtctggcgtt cggtggtgag aattcggggg actttcggtg
     1081 aagttcccat aaagttgcgt gcggtcaaag attcctgtca aagga
//
LOCUS       DATMKH010000393         1132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3066991, whole genome shotgun sequence.
ACCESSION   DATMKH010000393 DATMKH010000000
VERSION     DATMKH010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3066991"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            513..1028
                     /locus_tag="VNV63_04760"
     CDS             513..1028
                     /locus_tag="VNV63_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61962.1"
                     /translation="MKKVLLALAGLALAASMSVAQDVVSFFHGTVKKVDKTTKTVVVK
                     TKDGTEHTVKVASDDTVKGTKDGFDGIKEGSEVVVRSTGEGAEATVNGIGTVGKDGMK
                     VTKGTVEKIDDGTKTVVVKSADGTEKTFEYSGNALKDSGKAVGKGTEKGAKITVYYTE
                     EAGKKIAHFFE"
BASE COUNT          303 a          227 c          384 g          218 t
ORIGIN      
        1 agtctgtagc cgggcgtcat ttgtttcttg aataggaatg atttggttgt agaatccgcc
       61 gaatctggct aacggaggcg agtatgggac gcctgacggg actgggaaac atctgggcat
      121 caaacaggat gtgccgggac aaggagtttc gtttcgctgg aaagaggatg gcgatccgca
      181 ggggttcacc gcctggtcga ttttcaagcc ggacacaaag tattttcggc cgagcaccaa
      241 gccgttcatg ctgaattttc gcgtggaaga cctggacgga ctgctggagg ttgcggggcg
      301 aaggcgggtg gatcgatccc aatcgggcag actacgacta cgggcgattt gcctggatca
      361 tggacccgga aggaaaccgg attgagttgt gggaaccgcc gaagcactcg tgacagaggc
      421 aaacgtgcta aataattgaa tagtttaaca aaagcggtat gatggcccct ggaagtttgc
      481 aacgattagg tcctcggagg aggtatccgc agatgaaaaa ggtattgctg gcattggcag
      541 gactggcgtt agccgcgtcg atgagcgtgg cgcaggatgt ggtgagtttt tttcatggca
      601 cggtgaagaa ggtcgataag actacgaaga ccgtggttgt gaagaccaag gatggaacag
      661 aacacaccgt gaaggtcgcg agcgacgata ccgtaaaagg aacgaaagat ggtttcgacg
      721 gaatcaagga aggctcggaa gtcgtggtgc gttccaccgg ggagggcgca gaggcgaccg
      781 tcaatggcat cggaaccgtt ggcaaagacg ggatgaaggt gaccaaggga acggtggaga
      841 agatcgacga cgggacgaag acggtagttg taaaatcagc cgacggtaca gagaagacct
      901 ttgaatacag cggaaacgca ttgaaggatt caggcaaggc cgtgggcaag ggaacggaaa
      961 aaggcgccaa gataaccgtg tactacacgg aagaagccgg gaagaagatc gcgcactttt
     1021 tcgagtagga gcagaccttc gatccagttc gcgcgaatga aaaaggaagc ggccggggat
     1081 tgtcccggcc tcttttgttt tggagagcgg aaattggatt gagtggcggt tc
//
LOCUS       DATMKH010000394         1210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2420043, whole genome shotgun sequence.
ACCESSION   DATMKH010000394 DATMKH010000000
VERSION     DATMKH010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2420043"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..379
                     /locus_tag="VNV63_04765"
     CDS             98..379
                     /locus_tag="VNV63_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61963.1"
                     /translation="MLRITVVESSKVAVTLRVEGRITGPWVEELRTACKVHTFPDDVQ
                     LSLELADISFADAAGIALLRELRNRGVGLIRATPFLAEQLKEGASYLEF"
     gene            complement(508..855)
                     /locus_tag="VNV63_04770"
     CDS             complement(508..855)
                     /locus_tag="VNV63_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61964.1"
                     /translation="MMVLDHDVAELLSHTCHPDRALDCVNDVSDGGPKSVTTLGALKQ
                     RSDQFIHPLDGPTDFLVKFVPFCFADMRLGEKFCIRQDGGQRKAKIVAKEGHHLAYVG
                     LRLRTWNISLHAL"
     gene            838..>1210
                     /locus_tag="VNV63_04775"
     CDS             838..>1210
                     /locus_tag="VNV63_04775"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXC61965.1"
                     /translation="MIKHHHKGLAAGWFDSNRLERVVANLVLNACEAVSPDSGRIVIT
                     TTGNQTCLQIGVWDNGPGIHPAIQDSVFQPSVSYGKAEGSGLGLAIAKKIVEDHGGEI
                     YLDGESETGTLFKITIPFCHSG"
BASE COUNT          275 a          311 c          351 g          273 t
ORIGIN      
        1 tcggagcgga tctgggagat cgtttcaggc ctggtctgat cttgtggatg gcatgccaat
       61 tgcttggttg agtccagagg cgatcgggtg aaaggcgatg ctgaggatca cggtagtcga
      121 atcgtcaaag gtcgctgtaa ctcttcgggt tgaaggccgg ataacgggtc cctgggtcga
      181 agagctgcgc acagcatgca aagtgcacac tttccccgat gacgtgcagc tatccctcga
      241 gctggcggac atctcgtttg ccgacgcagc gggaatcgca ctcctcagag agctgaggaa
      301 ccgtggcgtt ggtctcatcc gggctacgcc gtttttggcc gaacagctca aagaaggggc
      361 ctcatatctc gaattttaga gcacagaccg gtatacgccg aatccggaaa ggaccctctc
      421 tttctgaact tcggccatcg cagacgggag aggtattcaa tgctggagca agctgagtgt
      481 agggagcggt tgttgcctat tggatcttta gagtgcatgc aaagagatat tccaagtccg
      541 gagccgcagt ccgacgtagg caagatggtg gccctccttt gccacgatct tcgccttcct
      601 ttgaccgcca tcctggcgaa tgcagaattt ctcacccagt cgcatatcag cgaaacagaa
      661 aggaacgaac tttaccagga aatccgttgg tccatcgagc ggatgaatga actggtcact
      721 tcgttgtttg agtgctccaa gggtcgtgac acttttcggc ccgccgtccg aaacatcgtt
      781 gacacagtcg agcgcgcgat ccggatgaca agtgtgagaa aggagttccg ccacatcatg
      841 atcaagcacc atcataaagg tttggccgcg ggctggttcg attccaaccg tctcgaacgg
      901 gtggtcgcca acctcgtctt gaatgcttgc gaggccgttt ctccggactc gggacggatc
      961 gtcataacca caaccggaaa tcaaacctgc ttgcaaattg gcgtttggga taacggcccc
     1021 ggcatccatc ctgcgattca ggactccgtc tttcaaccct ctgtgagcta cggaaaggcc
     1081 gagggcagcg gcctgggcct cgcaatagcg aagaagattg tcgaggatca tggcggggag
     1141 atttaccttg atggggaaag tgagaccgga actttattta agatcacgat tccattttgc
     1201 cattccggag
//
LOCUS       DATMKH010000395         1182 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3068973, whole genome shotgun sequence.
ACCESSION   DATMKH010000395 DATMKH010000000
VERSION     DATMKH010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1182
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3068973"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="VNV63_04780"
     CDS             complement(<1..579)
                     /locus_tag="VNV63_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008919785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXC61966.1"
                     /translation="MFTTKSGEKIFVVDAHTHLWDGSPANQKNIHGKQFIDCFYDYHH
                     LGPDSYIWPKEKFDRYTEEDYLKDVFEEGYADVAIFNPQILGDFYHHGFSNLKRNEAL
                     REQHPDRIIANGVWDPRDEEKGLELLERDAKAYHWKGVKLYTAEWRGDSKGWKLTDPW
                     SYRYLEKCRELGIKNIHVHKGPTIRPLNKDAFD"
     gene            complement(606..>1182)
                     /locus_tag="VNV63_04785"
     CDS             complement(606..>1182)
                     /locus_tag="VNV63_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007741897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HXC61967.1"
                     /translation="AGLTAYHVAKKAAQHLLPGQRVVVIGVGGLGHIGIQVLSALCSA
                     DIIAIDRSDLALTLAKECGAQHVVRADQNQVEAVRALTDGKGAEAVIDFVGEGNSIEK
                     GLAMTRNNGAYYIVGYGGEIRISAIDMITTERRIIGNLVGSYAELVELMELADRGKVH
                     LATKYYQLSEANQALHDLHHGKVRGRAVLIP"
BASE COUNT          253 a          327 c          297 g          305 t
ORIGIN      
        1 gtcaaaagca tctttattga gcgggcggat cgtcggaccc ttatgcacgt ggatattctt
       61 aattccgagc tcccggcatt tctccaagta tcgataggac cagggatcag tcagcttcca
      121 acccttggaa tctccgcgcc attcggcggt gtagagcttc acccctttcc agtggtaggc
      181 tttggcgtcc ctttccagca gttccagacc tttttcttca tcgcgcgggt cccaaacacc
      241 gttggcgatg atgcggtccg gatgttgctc tctgagtgcc tcatttcgtt tcaggttgga
      301 aaatccgtgg tgatagaagt cgcccagaat ttgtggattg aaaatcgcga cgtcagcgta
      361 gccttcttca aagacgtctt taagatagtc ttcctcggta tagcggtcga atttctcttt
      421 gggccaaata taagaatcgg ggcccaaatg atgataatca taaaagcagt cgataaactg
      481 ctttccgtga atgttcttct ggttggcggg actgccgtcc cacaaatggg tgtgggcgtc
      541 gaccacaaat atcttttctc cggatttggt agtaaacatg gtgatagttt tctttgagct
      601 gcttgttaag gtatcagaac tgcccggccc ctgaccttgc catgatgcag atcgtgcaac
      661 gcctggttag cctcactgag ctggtaatat ttggtcgcta aatggacctt tccccgatca
      721 gctaactcca ttagttccac cagctccgca taactcccga cgagattccc gataattctt
      781 cgctccgtgg tgatcatatc gatagcggaa atgcggatct cgccgccata acccacaata
      841 taataggcgc cgttgttccg tgtcatggcc agccccttct cgatggagtt tccttccccg
      901 acaaaatcaa tgaccgcttc cgcacctttt ccgtcggtca gcgcccgaac cgcttcgacc
      961 tggttctgat cggcccggac gacatgttga gcgccgcatt ctttggccag ggtcaacgcg
     1021 agatcggacc ggtcgatggc gataatatca gcagaacaga gggcgctgag gacctggatc
     1081 ccgatatgac cgagcccgcc cacgccaatc accaccacgc gttggccggg aagaagatgt
     1141 tgtgcggcct tcttggctac atggtacgca gtcaggcccg ca
//
LOCUS       DATMKH010000396         1033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4019265, whole genome shotgun sequence.
ACCESSION   DATMKH010000396 DATMKH010000000
VERSION     DATMKH010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4019265"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="VNV63_04790"
     CDS             complement(<1..278)
                     /locus_tag="VNV63_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HXC61968.1"
                     /translation="MKIGVPKEIKEQESRVAALPSVVYQLTRLGHQVYVERGAGVGAG
                     YPDKEYEDAGAIMSSVHAEIFREGDLIVKVKEPLPSEYPLLREGQILFT"
     gene            complement(292..852)
                     /locus_tag="VNV63_04795"
     CDS             complement(292..852)
                     /locus_tag="VNV63_04795"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HXC61969.1"
                     /translation="MRPSGSEIPNLTGQLLVAHPNLRDPNFRKTVLFLSAHDAEHGAH
                     GLVLNRPTNKSLTDFLPDHELDALGEVPVYLGGPVGAQELTIVSFDWVAGEAKITFHS
                     QLSLDQVQTLAGSGSKLIRAFVGYAGWAGGQLESELQQSAWIIHPATESILGDASNHE
                     TWLGLMKNLGPAYHLLAIAPDDPSLN"
     gene            922..>1033
                     /locus_tag="VNV63_04800"
     CDS             922..>1033
                     /locus_tag="VNV63_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013064977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HXC61970.1"
                     /translation="MHSTISKLVIADRSFESRLLVGTGKFANNSLMRDALE"
BASE COUNT          247 a          282 c          267 g          237 t
ORIGIN      
        1 gtaaacaaga tctggccttc tcgcaaaagc ggatactcgg aaggcagcgg ttccttgacc
       61 ttcacgatca gatctccttc ccggaagatc tctgcatgga cgctcgacat gattgcgcca
      121 gcatcttcgt actctttgtc cgggtagcct gcaccaactc ccgcgcctcg ctcgacgtaa
      181 acctgatggc ctagcctggt gagttggtaa acaaccgatg gaagagcagc gaccctgctt
      241 tcctgctctt tgatctcctt gggaactcca atcttcataa atgagaggac actaattcag
      301 cgagggatca tccggggcta tggcaagcaa gtgataggct ggtccaagat ttttcataag
      361 cccgagccag gtctcatgat tcgatgcgtc acccagaatc gactcggttg ctggatgaat
      421 gatccaggcg ctctgctgga gctcggattc cagttgccct cctgcccaac ccgcatatcc
      481 gacaaacgct cgaatcaatt tcgagccgct tccagccagg gtttgcactt gatccaaaga
      541 aagctgcgaa tgaaaggtaa ttttcgcctc gccggcgacc caatcgaagg aaacgatcgt
      601 caactcttgc gcgccgaccg gtcctccaag atagacgggc acttcaccga gcgcatcgag
      661 ttcgtgatcc gggagaaagt cggtcaggct cttgttggtc ggacggttca ggacgagacc
      721 gtgagctccg tgctcagcgt catgagcgga aagaaacaac acagtcttcc gaaaattcgg
      781 atcgcgtaag ttcggatgcg ccaccaatag ctgaccagtc agatttggaa tttcgctacc
      841 ggatgggcgc atttcagaat ctaggaagcg attacttgct gttcaagggc ggagtcagta
      901 attgtcctga ggttccattc catgcatagc acaatttcga aattagtaat cgcggaccgt
      961 agcttcgaat ccaggcttct cgtcggtacc ggcaaatttg cgaacaactc cctgatgcga
     1021 gatgctctgg aag
//
LOCUS       DATMKH010000397         1066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3390208, whole genome shotgun sequence.
ACCESSION   DATMKH010000397 DATMKH010000000
VERSION     DATMKH010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3390208"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="VNV63_04805"
     CDS             complement(<1..438)
                     /locus_tag="VNV63_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61971.1"
                     /translation="MRILWVKVGGLWPLNTGGRIRSFHLLSELSCRHEVTLLTTHGPG
                     DDLKGLADQLPHCKRIVSFPCNVPKQGTVRFAAALIRSWFSKLSVDLWKWRIPAVRDE
                     VSRLMSSQAVDLCVADFLYAVPNVPLGGPVPVVFFEHNVEYVIR"
     gene            complement(435..>1066)
                     /locus_tag="VNV63_04810"
     CDS             complement(435..>1066)
                     /locus_tag="VNV63_04810"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HXC61972.1"
                     /translation="SLFYDNFAVFTESFQECLLSDTEFLSACDPYAEGLRCYQEATGG
                     TLERMSRTDLQTYLVELLMKQDQMSMAASIESRVPFLDHKFVEYVLAIPGHFKLRRWQ
                     TKAVLREALRHLVPQEILTRGKMGFPVPVGRWLRGEFRSIIEEFVLGPRALQRGLFDP
                     SMLRLLAEEHRTGAREHGDRLWLLMNLEIWQRIFLDGEDPAFIIRAIQK"
BASE COUNT          258 a          329 c          274 g          205 t
ORIGIN      
        1 ccggatcacg tattccacat tatgctcaaa gaagacaacc gggacggggc cgcccagagg
       61 aacattcggg actgcgtaca gaaaatcagc cacacacaga tccactgctt gggaggacat
      121 caggcggctg acctcgtctc ggactgcagg aatacgccac ttccaaagat ccaccgaaag
      181 ctttgagaac caggatctta ttaatgccgc ggcaaagcgg acagttccct gcttaggaac
      241 gttgcaggga aacgacacga tgcgtttgca atgcggcagc tgatcggcca gccctttcag
      301 atcgtcgccc ggaccatgcg tggtcagcag ggtcacttca tgccggcagg aaagctccga
      361 cagaagatga aaacttcgga tccgccctcc tgtgttcagc ggccacaggc cccctacttt
      421 gacccagagg attctcattt ttgtatcgcc cggatgatga aagccggatc ttcaccatcg
      481 agaaagatcc tctgccagat ctcaagattc atgaggagcc agagtcgatc cccatgttcc
      541 ctggcacccg ttcggtgctc ctcggcaaga agccgaagca tggaaggatc aaacagccct
      601 ctttggagcg cccgcggtcc gagtacaaac tcctcaataa tcgatcggaa ctctcctcgg
      661 agccaacggc cgaccggaac cggaaacccc atcttccccc gcgtgaggat ctcttgcggt
      721 accagatgcc ggagcgcctc cctcagcacc gccttggtct gccacctccg aagcttgaaa
      781 tggccaggga tcgccagcac gtactcaaca aatttgtggt ccaggaaggg cactcggctt
      841 tcaatcgacg ccgccatact catctgatcc tgtttcatca gcaactccac caggtaggtc
      901 tggagatccg tccgactcat ccgctcaagc gtcccccccg tggcttcctg gtagcaacgg
      961 agtccctcgg cgtaagggtc gcaggcgctc agaaattcag tgtctgacag caaacattcc
     1021 tggaaagact cggtgaagac cgcaaagttg tcgtagaaaa gcgatc
//
LOCUS       DATMKH010000398         2504 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3691017, whole genome shotgun sequence.
ACCESSION   DATMKH010000398 DATMKH010000000
VERSION     DATMKH010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3691017"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..821
                     /locus_tag="VNV63_04815"
     CDS             <1..821
                     /locus_tag="VNV63_04815"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HXC61973.1"
                     /translation="SNEKLYNGGRMDRGMNKKRWGWGFVTACLVWIVLGSACQAQEDR
                     LSRIEKELAEIKADVTAIRQILAGAAIQGPQGPPPAEREVNVGDGPMRGNPKAKVTLI
                     EFSDYQCPFCGRFFNETLPQIDKEFIQTGKVRFVMRDLPLADIHPNAQKAAEAAGCAG
                     EHGKYWQMHDKLFQNQKALEMPRLKEYAKAIGLDGRAFDECLASGKQAVKIQRDLQEA
                     RNLGITGTPAFFLGLTKSGKTIKAISIEGAQPIDTFRQEINHLLSQADQPPKQP"
     gene            complement(957..1628)
                     /locus_tag="VNV63_04820"
     CDS             complement(957..1628)
                     /locus_tag="VNV63_04820"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HXC61974.1"
                     /translation="MKHNNVSPGWNWWSMMRTKNVECRYDWERGFAIVELMVTLVIAA
                     ILIAAVYRLYQSTQSGYLAQNQLTELQQNARIGIDTMIREIRLAGYQAYCDPVTANAA
                     MFEFEADTDLSGKPERIIYKLNAANKQLLRASSGDLASSIGCSSYDATATSNNLYQSV
                     ASNVTSLNFAYYDTNNGPPSDPSKVRRITINLTVSAVKAEQGGATRSVQITSDVMLRN
                     EGLKK"
     gene            complement(1686..>2504)
                     /locus_tag="VNV63_04825"
     CDS             complement(1686..>2504)
                     /locus_tag="VNV63_04825"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXC61975.1"
                     /translation="GQPLELKGGTMRFGTSFTSLLGTGMIVMASALGAGFILIYSAAE
                     HLVLGDERQERGDLDGAITEYRTALRLNSKYVLAHHNLGVALAKKGNLDGAIEEFRTL
                     LHLKPNLTDAHYGLGAVLAERGDLDEAIAEYRTVLRLDPNHLGAHDGLGLALKQKGDL
                     DGAIEELRTALRLYPNNAPFHDHLGRALYDKRDVDGGIEEYRNAIRLDPNYANAHYYL
                     ELALMDQGNTSAAAKELDEFIRLAPNIPAYRQKIDEAKRRLEKLKSPENSSGNP"
BASE COUNT          606 a          626 c          661 g          611 t
ORIGIN      
        1 gatcgaatga gaagttatat aatggaggca ggatggatag agggatgaat aaaaaaagat
       61 ggggatgggg attcgtaact gcctgtctgg tatggatcgt cttgggaagc gcatgccagg
      121 cgcaggagga ccgcttgagc cgaatcgaga aagagttggc cgagatcaag gcggatgtga
      181 cagcgattcg tcagatactt gccggggcgg cgattcaggg tcctcagggt ccgcccccgg
      241 cggagcgcga agtgaatgtc ggcgatggac cgatgcgtgg gaatcccaag gcgaaggtga
      301 cactgataga gttttcggac taccaatgcc cgttttgcgg gagatttttt aatgagactt
      361 tgccgcaaat agataaggag ttcatccaga ccggtaaagt ccgttttgta atgcgcgacc
      421 ttccattggc agacatccat cccaatgcgc agaaagcggc cgaggcggcc gggtgcgccg
      481 gggaacatgg aaaatactgg cagatgcatg ataagctttt ccaaaatcaa aaggccctcg
      541 agatgccccg tctcaaagag tatgcgaagg cgatcggatt agacggccgt gcctttgatg
      601 aatgcctggc cagcggtaag caggccgtaa aaatccaacg ggatttgcag gaagccagga
      661 acctgggaat caccggtaca ccggctttct tcttgggtct caccaaatcc gggaaaacga
      721 ttaaggcgat cagcatcgaa ggtgctcagc cgatcgatac attccgccaa gagatcaatc
      781 atctgctcag ccaggcggat caacccccga aacagccgta agaagtcatg acgggaagac
      841 acaaagatag ggtttcaccg ttttaaaatg tgcgagctga atgatcaggg taaattaaaa
      901 cggtccggac gcgattcttc cgaatcgtgc gtcaccagcg gtccggctcc tgtcttttac
      961 tttttaaggc cctcattccg cagcatcaca tcgcttgtga tttggacaga ccgggtggca
     1021 ccgccttgtt ccgcctttac ggcggacact gtaaggttga ttgtaatccg acggacctta
     1081 cttggatcgc taggaggacc attgttcgta tcataataag caaagtttaa cgatgtgaca
     1141 ttcgatgcaa cggactggta gaggttgttt gaagtagcag tggcatcata agagctgcag
     1201 ccgatgctcg aagccaaatc tcccgaggaa gcccgcagga gctgtttatt cgcagcattg
     1261 agtttgtata tgatccgttc cggcttaccg ctgagatctg tgtccgcctc gaattcgaac
     1321 atcgcggcgt tcgcggtgac tggatcgcag taggcctggt agccggccag gcgtatctcg
     1381 cggatcatcg tatcaatacc tatcctggcg ttctgctgaa gctcggtcag ttgattctgc
     1441 gccaggtagc cactctgagt ggattgatag agccgataga cggctgcgat caggatcgcc
     1501 gcaatcacca gggtcaccat gagttcgact atggcaaaac ctctttccca gtcgtatcgg
     1561 cattctacgt ttttggtcct catcatgctc caccaattcc aacccggcga cacattatta
     1621 tgtttcatcc agtgcgccgc cagccttaat tcttgaacct cgacttagat aaagctaaga
     1681 ccttttcagg ggttccctga tgaattctca ggtgatttaa gcttttcaag tcttctcttt
     1741 gcttcatcaa ttttttggcg ataggcaggg atattcggag ccagtcttat gaactcatct
     1801 agctcttttg ctgctgctga cgtgtttccc tgatccatta gcgcaagctc gagataatag
     1861 tgagcgtttg catagttcgg gtccaggcga atagcgttgc ggtactcctc aattcctcca
     1921 tcaacgtccc ttttgtcata aagcgcacgc ccgaggtgat cgtggaaggg tgcattgttc
     1981 gggtataatc gtagagcagt gcgcaactct tcaattgccc catcaaggtc ccctttttgc
     2041 ttgagggcaa gtccgagacc atcgtgagcg ccaagatggt tcggatccag acgaagaaca
     2101 gtccggtatt ctgctattgc ctcatcaagg tcccctcttt ctgcaagcac cgctccaaga
     2161 ccatagtgag cgtccgtaag attcggtttc agatgaagaa gggtgcgaaa ctcctcaatc
     2221 gccccatcaa gattcccttt tttagcgagc gcgactccga gattatggtg ggcgagtaca
     2281 tactttgaat ttaaacgaag agcggtgcgg tactctgtaa tcgccccatc gagatctccc
     2341 ctctcttgac gttcgtctcc taggaccaaa tgttctgcag cactgtatat taagatgaat
     2401 ccagcgccca gtgcactggc catcacgatc ataccggttc ctaataagga agtgaacgaa
     2461 gtgccaaatc tcattgttcc acctttcaac tccagcggct gacc
//
LOCUS       DATMKH010000399         1402 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2753249, whole genome shotgun sequence.
ACCESSION   DATMKH010000399 DATMKH010000000
VERSION     DATMKH010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2753249"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VNV63_04830"
     CDS             <1..232
                     /locus_tag="VNV63_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61976.1"
                     /translation="MRMSSLVVGLAATSIGISEGTANKAVMLRVYHGNDFANYYLDPP
                     YWSYRPKRFGHPGSSHWLYKNHRDVFIDIQVN"
     gene            complement(320..1204)
                     /locus_tag="VNV63_04835"
     CDS             complement(320..1204)
                     /locus_tag="VNV63_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61977.1"
                     /translation="MNQNLDLLRQRYCRPAVATSKATNSLFPLEKECEQGRTLLAKAP
                     KTLLQLQEFSPYGLHQTYRDEAAGLELPVFAVFDLEGDHQLTFEITTDSIAPATVPKS
                     LPSYIPFQTAQASVRKINKVRLRSERAVGRISVILGIVPVLAYFFSGVTALPSMVEAG
                     ILVGGWLVASFLSYVLGLSLLDRFCPWKKLVMTAEFNGILPKQTREKAVAAKSHFDRL
                     YVIIDQQHRWKSALLPDPRPRALDPLLIGELSQGRSRKFYLIDQFDLTEAEQYLADEF
                     AVKSEDEFGPAFGCDREQ"
BASE COUNT          330 a          358 c          386 g          328 t
ORIGIN      
        1 gatgagaatg agtagcctgg tagtaggatt ggccgcaaca tccattggga tctctgaagg
       61 aactgcaaac aaagcagtga tgcttcgcgt gtaccatgga aatgactttg ctaattatta
      121 tttagatcca ccgtattgga gttatcgccc taagcgattc gggcatcctg gaagctctca
      181 ttggctctat aaaaatcatc gagacgtctt tatcgacatc caagtaaatt aggcgcgcgg
      241 tgttggcggc ccgcagggcg gtggggcgag gctccgtccg aaccggatga ccgctcgcag
      301 tccagggtag atcattgagt cattgctccc tgtcgcaccc gaacgcaggg ccaaattcgt
      361 cctctgactt gacggcaaat tcgtccgcga gatactgctc ggcctcggtc agatcgaatt
      421 ggtcaatcag gtaaaatttc cgggaacggc cttgcgaaag ctcaccgatc agaagcggat
      481 ccaaagcacg tggcctggga tcaggcagga gtgcactctt ccaccggtgc tgctggtcaa
      541 ttatgacata aaggcggtcg aagtggcttt tggcggccac tgctttttcc cgagtctgtt
      601 tcgggaggat accgttaaat tccgcggtca tgaccagttt tttccaaggg caaaaccgat
      661 ccagcaagga gaggccgagt acgtagctca ggaaagaagc aacgagccac cctcccacca
      721 gaattccggc ctccaccatc gaaggcaagg cggttacccc cgaaaagaaa taggccaaga
      781 cagggacaat tcccaggatg acagagatgc ggcccacggc tcgttcgctc cttagtctga
      841 ctttgttgat cttcctgacg gatgcttggg ccgtctgaaa tggaatgtac gagggaaggc
      901 tctttggcac ggttgccggt gcaatcgaat ccgtggtgat ctcgaaagtg agttgatgat
      961 ctccctcaag gtcaaagacc gcgaaaacag gaagctccaa accggccgct tcatctcgat
     1021 aggtctgatg cagaccatag gggctaaact cttgaagctg caggagcgtc ttcggcgctt
     1081 tagcaagaag agttctcccc tgctcgcact ctttttccaa aggaaaaagg gaattggttg
     1141 ctttactcgt ggcgacggcg ggccgacagt atctttggcg cagcaggtct aggttctggt
     1201 tcattggagc tcctggattt ttaatcttct ttttcatcct cgcggttgga agggatcctg
     1261 aatggaagaa cgaatgcctt ccaaatggtc gccgaagctt tcgccagacg caactgggtt
     1321 catacgtttt cccgcaatcc ggaatatcgt ttccgctgcg atctgtctcg tgcctaccga
     1381 ggaacacagt cttgacgagg cg
//
LOCUS       DATMKH010000400         1106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4665177, whole genome shotgun sequence.
ACCESSION   DATMKH010000400 DATMKH010000000
VERSION     DATMKH010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4665177"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..907
                     /locus_tag="VNV63_04840"
     CDS             <1..907
                     /locus_tag="VNV63_04840"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXC61978.1"
                     /translation="YVAIHVNNMPDFLVFATIIPRLFGAKVILDIHDPMPNTFASKFK
                     GRESGFFYRALLLQERLSAAFCNRVITVHHPVKEGILIKHGLAPESIEVIANFADDKL
                     FSPQKSFSLDGKIRLVFHGTILERSGLRMLINSLARVRHKDRITLRIIGEGDFSQPLK
                     QMIQSLGLGDIVDFDNRSYPVHSIPERIADCNVGIVPLEISSVTNYALPLKLVEYISM
                     GLPVIAVRNEAISYYLKEEDCIFFDWNDPNSLSAALDRIAENPETLVHYRERSLALRD
                     GFSWSGEKRKYVALLRELTGADGNT"
BASE COUNT          262 a          276 c          295 g          273 t
ORIGIN      
        1 gtacgtggcc atccatgtca acaacatgcc ggatttcttg gtgttcgcaa ccattattcc
       61 tcgactgttc ggagccaagg tgattctgga tattcacgat ccgatgccca acacgtttgc
      121 ctcaaaattc aagggccgtg agagcggttt tttttatcgg gcgcttctct tacaggaacg
      181 gttaagcgca gcgttctgca atcgagtcat caccgtgcac cacccggtca aggaaggcat
      241 cctaatcaaa catggccttg ctcccgaatc gatcgaagtg atcgccaact tcgcggatga
      301 taagctcttt tcacctcaga agtcctttag tcttgacggc aagataagac tcgtctttca
      361 tggaacgatc ctcgagcgtt cgggactccg gatgttgata aatagcctgg cacgggtccg
      421 ccacaaggat cgcatcactc tcagaatcat tggggaggga gatttcagcc aaccgctgaa
      481 gcagatgatc cagtcattgg gactggggga catcgtcgat tttgacaatc gaagctatcc
      541 cgtgcattcc attccggagc ggatcgcgga ctgtaatgtc ggcatcgtac cgcttgagat
      601 ttcctctgtg actaactacg ctctgccttt aaagttagtc gagtatatct caatgggact
      661 gccagtcatc gccgtgcgga acgaggcaat ttcgtactac ctcaaagagg aagattgtat
      721 tttctttgac tggaacgatc ccaactcatt gagtgccgct ctggaccgca tagctgagaa
      781 ccctgagact cttgtacact accgggagcg ctctcttgcc ttgcgcgacg gtttttcttg
      841 gtccggagag aagcgaaaat atgtagcatt gctccgcgaa ctcacgggtg ctgacggaaa
      901 cacttagggc gcccttagca ttgaggatag cgggacagaa gaaatcggca taaaggaggt
      961 ctattgaaat gtttaaggtt tgcgacaagg atatcaaagt cgagggacgg atgctccgga
     1021 ttgctcgctt ggacggggat agatacactt tcccggacga tccggaggcg gtgattcgtg
     1081 ccctgcggaa cttgggaacg cgcgtc
//
LOCUS       DATMKH010000401         1810 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_827772,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000401 DATMKH010000000
VERSION     DATMKH010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827772"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1215
                     /locus_tag="VNV63_04845"
     CDS             <1..1215
                     /locus_tag="VNV63_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012462437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXC61979.1"
                     /translation="IREENAQLVGYVFVDITSSDIEGYVRAAAKQIAQKVHFPQGYYI
                     QWAGQFEYLKAAEGRLAVLVPFTLLIIFFLIYLNTRSVIKTFIVLLSVPFSLVGAFWM
                     LYLLHYNMSVAVWVGLIALAGLDAETGVVMLLYLDHAWDKFKAQGRMNRIEDLYAAVI
                     EGAVQRIRPKIMTVCAIIFGLLPIMWSPVYQAGADVMKRIATPMIGGVITSAILNLLI
                     YPVIYVIWKKRSLPAQPPAGSFEGSAVPESRAVASGSRRRVLAGSMALFLVGIVIASA
                     YFAWASGIGNSIFRSADPKMPVAEVTQDGVRVSILSADGQIHAKDNALQIKFQDPGGH
                     PIDAENVKLELNMNMPGMEMHSGAKINKDSEMGDYKAHLTPEMAGDWSVDLSYQGPQG
                     PAKLKVPVNVKQ"
     gene            1450..>1810
                     /locus_tag="VNV63_04850"
     CDS             1450..>1810
                     /locus_tag="VNV63_04850"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXC61980.1"
                     /translation="MERWEKSLHSFILRYVGNYADSIELAEETFVRVYHHRSRFNFQS
                     KFSTWLLAIATNLCRHHARWRMRHPTISLDDAANIDEVSDENLFISPEEQPSALADRS
                     ELAKLVKEEIEKLPHDLK"
BASE COUNT          431 a          449 c          477 g          453 t
ORIGIN      
        1 attcgggaag agaatgccca gctagtgggt tatgtgttcg ttgatattac aagtagtgat
       61 atcgagggat acgtgcgggc cgcggcaaag cagatcgctc agaaagtcca ttttccgcag
      121 ggttattata ttcagtgggc gggtcaattt gaatacctga aggcggccga aggacgccta
      181 gctgtcttgg tgccgtttac cctattgatc atttttttcc tgatctatct gaacacgaga
      241 tcagtcataa agacctttat cgtccttttg tcggtaccct tctcgttggt gggggcattc
      301 tggatgttgt acctcctgca ttacaatatg agcgttgcgg tgtgggttgg cctgattgct
      361 cttgccggtc ttgatgccga gaccggagtg gtcatgctcc tttatctcga ccacgcctgg
      421 gataagttca aagcgcaagg acgcatgaac aggatcgaag atctgtacgc cgccgtcata
      481 gaaggtgcgg tccagcgaat tcggccaaag atcatgaccg tatgcgcaat tattttcgga
      541 ctactaccga tcatgtggtc accggtctat caagctggtg ccgatgtgat gaaacggatt
      601 gctactccga tgatcggcgg cgtaattacg tcggcaattc tcaatctctt gatctatccg
      661 gtaatctatg tgatctggaa aaagcggtca cttcctgctc agcctcccgc ggggtcgttt
      721 gaaggatccg ccgttcctga atccagagcc gtcgcttccg gttctcggcg tcgggtgctt
      781 gctggttcca tggccttgtt cctcgttgga atcgtgatcg caagcgctta ttttgcatgg
      841 gcctcaggga tcgggaattc gatctttaga tcggccgatc caaagatgcc tgtcgccgag
      901 gtaactcagg atggtgtgag ggtttccatt cttagcgccg acggccagat ccacgccaaa
      961 gacaacgctt tgcagatcaa gtttcaagat ccaggcgggc atcccatcga cgcagaaaat
     1021 gttaaactgg aactgaacat gaacatgccc ggaatggaga tgcattcagg ggcgaaaata
     1081 aataaggaca gtgagatggg ggactacaag gcgcacctca cgcctgaaat ggcaggcgat
     1141 tggagcgtcg atctttccta ccagggtccc caaggaccgg cgaagctaaa agttccggtc
     1201 aacgtcaagc agtagggtgt ccggggttcc tgctccagtt tctcaaccgt attgggagat
     1261 ctaactcccg agcgaatgca aatagagagg agtctatcat cgatctgata gaatgagtaa
     1321 ttcccacaac agaccgggtt tcaatctgga gaatatccag ttgtcgtgaa cgcccctgac
     1381 cacaacgatc cagatcactc gtgcatggtc cgcctaatag aaggcgacga cctggcgctt
     1441 aatgagctaa tggagcgctg ggaaaagtcc ttgcactcgt tcatcttgcg atatgtcggc
     1501 aactacgctg actcaatcga acttgccgaa gaaactttcg tccgagtgta tcatcatcga
     1561 agccggttta acttccaaag caaattttcc acttggttgc tagcgatcgc caccaatctc
     1621 tgtaggcacc atgcccgatg gcgcatgcgg catccgacaa tctccttgga tgatgctgcg
     1681 aacatcgatg aagtttctga cgagaatctt ttcatctccc ctgaagaaca accttcggct
     1741 ctagcagatc gctcggaact cgctaagctt gtcaaagagg agattgagaa gctccctcat
     1801 gatctcaaag
//
LOCUS       DATMKH010000402         1278 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_828672,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000402 DATMKH010000000
VERSION     DATMKH010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_828672"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..903
                     /locus_tag="VNV63_04855"
     CDS             <1..903
                     /locus_tag="VNV63_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61981.1"
                     /translation="GPIPPANRVTLADSVIDVKRAAWIDQSHNYSTLWKTDEWASESF
                     NFGWSTNPENPPSSFSTAVVPPTTLSVIPPPQASGQIELITTNSGSALNPATPILLGI
                     PDDFCWVVRMGALADLLGREGQSRDEKRAQYAEARWKEGIALAKQFTSILTGYINGVQ
                     VFPQSVHALSAFRSSWQDEAPNTPDSIGVMSWNLIALVPLPISSAISIMLDMVQNAPV
                     PVADGDFIQIGREDLDALLDMAQHLLAFKQGGQEFMETVPLYQSAVTQAKLRNSKIKE
                     NIPFYEALDDRADVQEEETESKKG"
     gene            912..1166
                     /locus_tag="VNV63_04860"
     CDS             912..1166
                     /locus_tag="VNV63_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61982.1"
                     /translation="MGGILGGALGGFASEALDMKKGGKRGLLRGKKKTSPAPSPGPMA
                     DPLEYKRGGKVKKTGWAKVHKNEVVVPAKKRARRSRGKKR"
BASE COUNT          328 a          333 c          356 g          261 t
ORIGIN      
        1 ggaccgatac ctccagcgaa ccgagtaact ttggccgatt cggttatcga cgtgaagcgt
       61 gcggcatgga tcgaccagag ccacaactac tcgacccttt ggaaaacgga cgaatgggcc
      121 tcggagtctt tcaattttgg atggtctaca aacccagaga atccgccttc cagtttcagc
      181 acagcggtag ttccaccaac gaccctttct gtgattccgc caccgcaagc gagcggacag
      241 atcgaactta ttaccacaaa ttccggatcg gcattgaatc ccgctacacc gattctcttg
      301 ggcatccccg atgacttttg ttgggtggtt cgcatgggag ccttggccga cttgcttggc
      361 agagaaggtc agtctcggga cgaaaagaga gctcaatatg ccgaagcgcg ttggaaggaa
      421 gggatcgctc tcgccaaaca atttacatcc atcttgacgg gctatatcaa tggcgtgcag
      481 gtattccctc agtccgtcca tgcgctcagt gccttccgtt caagctggca ggacgaagcc
      541 ccgaacactc ccgactcgat cggagttatg tcttggaacc tgatagccct tgtcccgttg
      601 cccatttcct ccgctatttc tatcatgctg gacatggttc aaaatgcccc tgtgcccgtg
      661 gccgatggag actttatcca gattggccgt gaggatctgg atgccctact cgacatggcg
      721 caacaccttt tggctttcaa acagggcgga caagagttca tggaaaccgt tccgctttac
      781 caaagcgcag tcacccaggc caaactcaga aactcgaaga taaaagaaaa tattcccttc
      841 tacgaagctc tcgatgatcg cgcagacgtt caggaagaag agacagagag caagaagggt
      901 taggaggaca gatgggcgga atattagggg gagcactcgg gggatttgct tccgaagcat
      961 tggacatgaa gaagggcgga aagcgcgggc tcctgcgcgg aaagaagaag acttctcctg
     1021 ctcccagtcc cggtccaatg gccgacccgc tggaatacaa gcgcggcggc aaggtgaaga
     1081 aaaccggatg ggccaaagtt cacaaaaatg aagtcgtggt ccccgcgaag aagagagcac
     1141 gtcgctctcg cggaaagaaa agataggcag tgttcaagaa aaagagggga ggcagtaaaa
     1201 tggccgttcc atccacaggc gcagttcttg acgcagtggg caagcgggtt cgaggctcct
     1261 ttaagaaagg tggcgtcg
//
LOCUS       DATMKH010000403         1634 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1153588, whole genome shotgun sequence.
ACCESSION   DATMKH010000403 DATMKH010000000
VERSION     DATMKH010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1153588"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="VNV63_04865"
     CDS             complement(<1..636)
                     /locus_tag="VNV63_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HXC61983.1"
                     /translation="MKLRVLLLAVYGLLTPFLRAEEKELIVGMEMSFPPFEMLDSSGQ
                     PAGVSVEIARAMGQSLNRPVRFENIPFDGLIPSLKTGKIDLIISSLTVSEDRKKSIDF
                     SDPYLKMGLAILANKNTDIQSINDVDKPGRNVAVKKGTTGNIYAMQHFKNAKVLVFND
                     ESACAVEVGEGKSDCFIYDQISVFQNWRKHQDRTRAILQPFEQEEWAMGIRK"
     gene            752..1579
                     /locus_tag="VNV63_04870"
     CDS             752..1579
                     /locus_tag="VNV63_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXC61984.1"
                     /translation="MLEVRDLTISFRSDDGDIPAVRSVSFNVQKGETLAIVGESGSGK
                     SVTAMALTRLLPEPAALYETGEILLEGKSILHMDEKRLRGVRGNQIAYVFQEPATSLN
                     PVFSVYEQIAETIRLHRPDVREVRREVIHWLDQVGIVDSARRLKDYPFQLSGGMQQRV
                     MIAMALCCQPAILVADEPTTALDVTIQRQILDLFQALKMKFRMSMILITHNFGIIRGL
                     ADKVAVMFRGRVVEFGSTESILRQPQHPYTRALIECIPRLGLKLDRLKTIDYSTLNG"
BASE COUNT          377 a          407 c          452 g          398 t
ORIGIN      
        1 ctttcgaatc cccatggccc attcctcttg ttcgaaaggc tgcaggattg ctcgggttcg
       61 atcctggtgt ttcctccagt tttggaaaac ggaaatttga tcgtagatga aacagtctga
      121 ctttccctct cccacttcga ccgcgcacgc gctctcgtcg ttgaagacga gaacttttgc
      181 gttcttgaag tgctgcatcg cataaatatt gccggtggtc ccttttttca cggcgacatt
      241 gcgaccgggc ttgtcgacat cattgatcga ctggatatcg gtatttttgt ttgccaggat
      301 ggccagcccc atcttcaaat agggatcgct gaaatcgatc gattttttgc ggtcctcgct
      361 cacggttaac gaggaaatga tgaggtcgat tttacccgtc ttcaaagatg gaatcagtcc
      421 gtcaaatgga atgttttcaa accggacggg acggttcagg gactggccca tggcccgggc
      481 gatctccacg ctcacgcccg ctggctggcc gctcgaatct agcatttcga agggcggaaa
      541 cgacatctcc attcccacga tcaattcttt ttcttcggct cggagaaaag gggtgagcaa
      601 tccataaacc gccagcagca agacgcgaag tttcacaaaa cggacttcta ttcttcagcg
      661 atgcggtttt caatcacgga tatatttcgc cgtaaagcaa ttagccggtt ctcctcttta
      721 caagtgccgt cgctgatcct aatcaagaca gatgctggaa gtcagggatc tgaccatcag
      781 tttccgatcg gacgatgggg acattcccgc ggtgcggtcg gtgagcttca acgtgcaaaa
      841 aggagaaacc ttggccatcg tgggagaaag cggaagtggg aagagtgtga ccgccatggc
      901 cttgaccaga ctacttccag agccggcggc gctttacgaa accggtgaga tcctgctcga
      961 aggtaaatcg atcttgcaca tggatgaaaa acgactacgc ggggtgcgag gcaatcaaat
     1021 tgcctatgtt ttccaggagc cggcgacgag tttgaatccg gtgttttccg tttatgagca
     1081 gattgcagaa acgatccgct tgcatcggcc ggatgttcgc gaagtacgca gagaggtcat
     1141 ccactggctt gatcaggttg ggattgtgga ttcggctcgc cggttgaaag actatccatt
     1201 ccagttgagc ggcggaatgc agcaacgcgt gatgatcgca atggccctct gttgccagcc
     1261 ggccatcctg gtcgccgacg agccaacgac ggccctcgac gtgacgatcc agcggcaaat
     1321 cctcgatctc tttcaggccc tgaagatgaa attccgaatg tcgatgatcc tgatcacgca
     1381 caatttcggt atcattcgtg gattggcgga caaagttgcg gtgatgtttc gcggacgtgt
     1441 ggtggaattt gggagcactg aatcgatcct ccgtcagcca caacatccat atacgcgggc
     1501 cctgatcgag tgcattccaa ggttaggtct gaagcttgat cgcctgaaaa cgattgatta
     1561 ttcgacgttg aacgggtgag tcaggagtca ggagtcagga gtcaggagtc aggagtcagg
     1621 agtcaggagt cagg
//
LOCUS       DATMKH010000404         1002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1471039, whole genome shotgun sequence.
ACCESSION   DATMKH010000404 DATMKH010000000
VERSION     DATMKH010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1471039"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            189..938
                     /locus_tag="VNV63_04875"
     CDS             189..938
                     /locus_tag="VNV63_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HXC61985.1"
                     /translation="MLITATMDADYKVKIEVFEGPLDLLLYLIKREEVDIYSVSIERI
                     TNQYLEYLNTFRMLDLDIAGEFVVMAANLIYIKSRSLLPADQQAADEEADEEDPRWDL
                     IRQLIEYKKFKDVALHLHRREVLQEGIIPRVAGKPALEDAETLLKDEVGIFDLITAFR
                     KVLKRLENRREDLREIFEENFSVSEKIDYILQTISSGAKVAFSSLFATAASRTEIVVT
                     FLALLELIRLKQLRVTQQTAFADIEIERVGL"
BASE COUNT          259 a          248 c          260 g          235 t
ORIGIN      
        1 ctgctgatga ccgacgaatc aaggcccggg gagcagtaat cagtaatcag tgagccagta
       61 atcagttgtg atccgccgtt tcggagcgac catgcattcc aatccctctc tgagaggttt
      121 cattgttcgc cataaccgcg actgaatact gacatactga ttactgatta ctgattgctg
      181 attgctgatt gctgattacc gccacgatgg atgccgatta caaagttaag atcgaagtct
      241 tcgaaggacc gctagacctt ctcctttacc tcatcaagag agaggaggtc gatatttact
      301 cggtatcgat cgagcgcatc acgaatcaat atctggaata cctaaatacg ttccggatgc
      361 tggacctcga tattgccggt gaattcgtgg taatggccgc taacctcatc tacatcaaga
      421 gccggagcct attgccggcc gatcaacagg cggcggacga agaagcagac gaagaagatc
      481 ccagatggga cctaatccgg cagttgatcg aatataaaaa attcaaagat gtcgcgctcc
      541 atttgcaccg gcgggaagtt cttcaggaag ggattattcc gcgcgtggcg gggaagccgg
      601 ccttggaaga tgcggaaacc ctcctgaaag acgaagtcgg gattttcgac ctgatcaccg
      661 catttcggaa ggtgctcaag agactggaaa accggcgtga ggacctgcgc gagatcttcg
      721 aagagaattt tagtgtctcc gaaaaaatcg attacatcct gcaaacgatc agttccggcg
      781 caaaggtcgc tttttccagt cttttcgcca cggcagccag ccgcacagag atagtggtga
      841 cttttctcgc cctgctcgaa ttaatccggc taaagcaact gcgggtaacc cagcaaacgg
      901 ccttcgcaga catcgagata gaacgggtcg gattgtagcc ttttcccggt agggatgatc
      961 tcctgctcat ccggggctgt tttaacgaag atgcacgttt tt
//
LOCUS       DATMKH010000405         1002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1154063, whole genome shotgun sequence.
ACCESSION   DATMKH010000405 DATMKH010000000
VERSION     DATMKH010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1154063"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="VNV63_04880"
     CDS             complement(<1..220)
                     /locus_tag="VNV63_04880"
                     /inference="COORDINATES: protein motif:HMM:NF012872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase [NADPH] flavoprotein
                     alpha-component"
                     /protein_id="HXC61986.1"
                     /translation="MNREGSEKETWHYEFSLAGSGLAYEVGDSMGIHPQNNPQLVQEL
                     LDALHFSGEEQVKNKEGEAFSIREGLLKH"
     gene            complement(445..>1002)
                     /locus_tag="VNV63_04885"
     CDS             complement(445..>1002)
                     /locus_tag="VNV63_04885"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HXC61987.1"
                     /translation="AKILTPVIYVGSYIARSFLGLKRPREIFVAREDLKYVTSEIERM
                     GMLSSIERQMIHNVVDFRLVKVRDVMVAISDVVTVRPETTIEQLIDLSRRSRFDRYPV
                     VDSRGKIVGTVNVFDLIVDQPLISTVRDYIRRILTVRPDNQASIVLSRLRAAPSSLAA
                     VVDEQGNTIGIVSVEDLLGPLVKVG"
BASE COUNT          244 a          285 c          248 g          225 t
ORIGIN      
        1 agtgtttcag gagaccttcc cgaatggaaa aggcttcgcc ctctttgttt ttgacctgct
       61 cctccccgga aaagtgcaag gcgtcgagaa gctcctgcac cagttgcgga ttattctggg
      121 ggtggatacc catcgaatca ccgacctcgt aggccaagcc ggagccggcc agcgagaatt
      181 cgtagtgcca ggtctccttt tccgaacctt ccctgttcaa tttctggttg acgaggagca
      241 tcgccggaaa gggatttttg cgagaataag gacctgcgga cggggtggac atagggaagg
      301 aagtagtcag tggtcagtat tcgtcatcga gcaatctctc ttgcctgcag gccgacatcc
      361 atttcgcctc atataaatca atctctcctg caaatgcaaa tctgaccggc aactgacaac
      421 cgaccactga caacttgtcc cccctcagcc cactttcacc agaggaccca aaaggtcttc
      481 aacgctgacg atgccgatcg tgtttccctg ctcatcgacg accgcggcca ggctgctggg
      541 agccgcccgc agccggctca agacgatcga cgcctgatta tccggtctga ccgtcagaat
      601 acgccgaata taatcccgga cggtagagat tagcggctgg tcgacaatca gatcaaaaac
      661 attcacggtc ccgacgatct tgcctcgcga atcgaccacg ggataacggt caaatcgcga
      721 acgtcgcgaa aggtcaatca attgttcgat ggtggtttct ggacggaccg taaccacatc
      781 ggagatggca accatcacgt cccgcacctt gactaaccga aaatcgacaa cgttatggat
      841 catttgtcgt tcgatgctgc tcaacattcc catccgttca atctcgctgg tgacgtattt
      901 caaatcttcg cgcgcgacaa atatttcccg aggccgcttg agaccgagga agctcctggc
      961 aatataggat ccaacataaa tcaccggtgt tagaattttg gc
//
LOCUS       DATMKH010000406         1033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2425980, whole genome shotgun sequence.
ACCESSION   DATMKH010000406 DATMKH010000000
VERSION     DATMKH010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2425980"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..819)
                     /locus_tag="VNV63_04890"
     CDS             complement(<1..819)
                     /locus_tag="VNV63_04890"
                     /inference="COORDINATES: protein motif:HMM:NF026135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61988.1"
                     /translation="MSVGGGYPIRQILVALVVLPALYLDFNIYRTGNVWVALTMLIVI
                     GLGAFVYLSELGYTYRYLFPGFLGFGLFVIFPIVYMVFLSFTKYSSQNLLYFDRSLAL
                     LRQESFSVGNVSYKYRLYLQEDGKYILYLENDSDPTKRFVSEPFSLKPGEQPKDPQEP
                     TRLTALSPKEDVTGKALEFAQVTRQKLFIPMRGRAFALPDDTLVAMEGLQKFTSRDRL
                     WTFNPDGSLTNKQDGKIIRPDFKLGFFVTDKGEKVGAGFRTYSGFENYVRIVSDP"
     gene            complement(816..>1033)
                     /gene="malE"
                     /locus_tag="VNV63_04895"
     CDS             complement(816..>1033)
                     /gene="malE"
                     /locus_tag="VNV63_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="functions in the MalKFGE ABC transporter complex to
                     transport maltose into the cell by using ATP hydrolysis;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HXC61989.1"
                     /translation="VPALKTLSEEMAAKNPLIKTTYENAKNGDVMPNIPQMGKFWSSM
                     ATAFQIATNGQASPRAALDDAKKNMEK"
BASE COUNT          238 a          262 c          280 g          253 t
ORIGIN      
        1 tggatccgag acgatccgaa cgtagttctc gaatccactg taggtccgaa agcctgcgcc
       61 cactttttcc cctttatccg tcacaaaaaa gcccagcttg aagtctggac gtataatttt
      121 gccgtcttgc ttgttggtga gtgagccgtc tggattgaag gtccagagcc ggtcgcggga
      181 agtgaatttc tgcaatcctt ccatcgccac caaggtgtcg tccggcaaag caaatgcgcg
      241 tccgcgcatc gggataaaga gtttctgccg ggtgacctgt gcgaactcca gggcttttcc
      301 agtcacgtct tcctttgggg agagagcggt caatcttgtc ggctcttggg ggtctttagg
      361 ttgttctcct ggcttcaagc tgaacggctc tgaaacgaaa cgcttggtgg ggtcactgtc
      421 gttttccaag tagaggatat attttccatc ctcctgcagg tacaacctgt atttgtatga
      481 aacgttgcca accgaaaagc tttcctggcg tagcagcgcc agtgagcgat cgaaatagag
      541 cagattttgt gagctgtact tcgtgaagct caggaagacc atgtagacga tcggaaaaat
      601 gacgaagaga ccgaacccta aaaatcccgg gaaaagatag cggtaggtgt atccgagttc
      661 ggaaaggtaa acaaaggcgc cgagaccgat gacgatgagc attgtgagag ccacccagac
      721 attcccggtg cggtaaatat tgaaatccag gtaaagcgct ggtaagacca ccagcgctac
      781 cagaatttgc cgaatcgggt agcctccgcc gacgctcact tttccatgtt tttcttcgcg
      841 tcatccaggg cggcccgagg ggaggcctgc ccgttcgtgg cgatctggaa tgccgtcgcc
      901 atggaactcc agaacttacc catctgcgga atatttggca tcacgtcgcc gttcttcgca
      961 ttttcgtagg tcgtcttgat gagcggattc tttgcggcca tctcctcaga aagagttttc
     1021 aatgccggaa cgc
//
LOCUS       DATMKH010000407         1444 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1154101, whole genome shotgun sequence.
ACCESSION   DATMKH010000407 DATMKH010000000
VERSION     DATMKH010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1444
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1154101"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="VNV63_04900"
     CDS             complement(<1..469)
                     /locus_tag="VNV63_04900"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="part of NADH-ubiquinone oxidoreductase complex I;
                     shuttles electrons from NADH, via FMN and iron-sulfur
                     (Fe-S) centers, to quinones in the respiratory chain; NuoF
                     is part of the soluble NADH dehydrogenase fragment, which
                     represents the electron input part of NADH dehydrogenase;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit F"
                     /protein_id="HXC61990.1"
                     /translation="MKKQKIESVLLDKTKESGPLWLEEYQQAGGYEGLRKALTRMKPA
                     DVIAEIQKAGLRGRGGAGYPTWKKWEQVAKNNQTTRYLCANAGEHEPGTFKDRHLIRK
                     NPHLLVEGSLIAAYAVEARVAYLYVNGSFHEEIGIIKKAMAEAKAKGYWGENIL"
     gene            complement(479..982)
                     /locus_tag="VNV63_04905"
     CDS             complement(479..982)
                     /locus_tag="VNV63_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017251045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HXC61991.1"
                     /translation="MGLAEHREKAPKHVACMVITVSDTRTPENDTSGDLIKKLLSDRG
                     HRIEGYLVVRDDSKAVKAAILKGLSKAGVQAIILNGGTGISKRDGTYEVVSGLLEKRL
                     DGFGELFRYLSYQEIGSAAMMSRAIAGVCRGKAVISMPGSTSAVELAMKSLVLHELDH
                     MIGEVSK"
     gene            complement(1047..1394)
                     /locus_tag="VNV63_04910"
     CDS             complement(1047..1394)
                     /locus_tag="VNV63_04910"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HXC61992.1"
                     /translation="MTEQISLERVMDSNYDSFLQAPAGVLLFKIASCKACEEFEPIVQ
                     NISGTYHSSIRFGKVLMHVPGTCTGIKKRHSFETFPTTHFYKNGQLVQAIEGKLDEEE
                     LTRLIETHLLSSK"
BASE COUNT          299 a          416 c          351 g          378 t
ORIGIN      
        1 ccaagatgtt ctcgccccag tagcctttcg ccttggcttc ggccatcgct ttcttgatga
       61 ttccgatctc ttcatggaaa ctgccattga cataaaggta ggccacgcgg gcttccacgg
      121 cataggccgc gattagggat ccttcgacga gtagatgagg gtttttccgg attaaatgac
      181 ggtctttgaa ggtccccggc tcatgctctc ctgcattggc acagagatat cgagttgtct
      241 ggttgttctt ggcgacctgc tcccattttt tccaggtagg ataacccgcc ccgcctcggc
      301 ctcgtagtcc cgccttctgg atctcagcga tcacgtcggc tggcttcatt cgggtcagag
      361 ccttccttaa gccttcatac cctccggctt gctgatactc ttccaaccac aaaggtccgg
      421 actctttagt cttatccagc aggacgcttt cgatcttctg ctttttcacg ctgcgacctt
      481 attttgagac ttcgccaatc atgtggtcaa gctcatgaag taccaaggac ttcatcgcca
      541 gttccactgc cgaagtagaa ccgggcatcg agatgacagc cttcccccgg cagacacccg
      601 caatcgcccg gctcatcatc gcggccgagc cgatctcctg ataactaaga taccggaaca
      661 actcgccaaa accgtccagt ctcttctcga gaaggccgct caccacctcg taggtcccgt
      721 cacgtttcga tatcccggtt ccgccgttta aaatgatcgc ttgaaccccg gctttggaaa
      781 gtcctttgag tatcgccgcc ttcaccgctt tggaatcgtc acgaacgacc aggtaaccct
      841 caatgcgatg cccccggtcc gatagaagct tcttgatcaa gtccccgctg gtatcgttct
      901 ccggggtccg ggtatcgctc accgtaatca ccatacaggc cacatgcttc ggggcctttt
      961 ctcgatgctc cgccagtccc attatttcac cttggaaggt tcattttatg ccgtaagcgt
     1021 cggtgaagtc aaccgaatgg gtcaaactat tttgaggaga gcagatgggt ttcaatcagc
     1081 cgtgtcagtt cctcctcatc cagtttccct tcgattgcct ggacaagctg gccgttttta
     1141 tagaaatggg tggtggggaa agtctcaaag ctgtggcgtt ttttaatgcc agtgcaggtt
     1201 ccggggacgt gcatcaggac ctttccgaat cggatggagg agtgataggt tcctgagatg
     1261 ttctgaacga tcggctcgaa ctcctcacaa gctttacagg aagcgatctt aaagagaagc
     1321 acccccgccg gagcctgaag gaaggagtcg tagttggagt ccatcacacg ctcaagggat
     1381 atttgctcag tcattatatc gcttatctgt cttacttagc ctacttggcc cagcgattcg
     1441 agga
//
LOCUS       DATMKH010000408         2281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2104969, whole genome shotgun sequence.
ACCESSION   DATMKH010000408 DATMKH010000000
VERSION     DATMKH010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2104969"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1654)
                     /locus_tag="VNV63_04915"
     CDS             complement(<1..1654)
                     /locus_tag="VNV63_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HXC61993.1"
                     /translation="MEAKIENRYDADKIKVLEGLEAVRKRPAMYVGSTGPEGLHHLVY
                     EVVDNSVDEAMGGHATKIDATIHIDNSVTVIDDGRGIPTGMHTAQKRSAAEVVMTVLH
                     SGGKFDQEAYKVSGGLHGVGISVVNALSEWLELEIKQEGQVYQQRYQRGKPEAALEVT
                     GKTKKHGTKVTFKPDAQIFEVLEFSFDTLAQRLRELAFLNKGLEITLADERAEKRQVF
                     CYKGGIISFVEHLNENKSPLHKPVYIEREKNGIILEAALQYNEGYSENIFSFANNINT
                     REGGTHLIGFKTALTRCVNSYASANNLLKDETITGEDVREGLTAVVSIKLPNPQFEGQ
                     TKAKLGNSEAKGIVEAAVNEALSEFFEENPPIARKIVDKAVGAARAREAARKAKDLIR
                     RKNAMDGGSLPGKLADCSEKDPALSELFIVEGDSAGGSAKQGRDRRNQAILPLKGKIL
                     NVEKARFDKMISSEEIRILITALGTGIGQEDFDIGKLRYHKIILMTDADVDGAHIRTL
                     LLTFFYRQMPKLIEHRYIYIAQPPLFKIKKGKMERYIKNEGAM"
     gene            complement(1658..>2281)
                     /gene="dnaN"
                     /locus_tag="VNV63_04920"
     CDS             complement(1658..>2281)
                     /gene="dnaN"
                     /locus_tag="VNV63_04920"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HXC61994.1"
                     /translation="KKALRCVGTDGHRLAVIQRELGPQGSSNGENRDLIVPKKAVLEL
                     RRLLEEDNEDLTLGFGKNQLLIQKKKITLFTRLMEGTYPNYEQVVPKQNDKKVSVARQ
                     DLEGALRRVAVLSREKTNAIKLSLEPGRLTLISSSPDLGEAKETMAVSYKGEGLQTGF
                     NARYLLDVLGAFESDQAVLEFKDALSPCLIREGKDPGYLCVVMPMRV"
BASE COUNT          431 a          690 c          560 g          600 t
ORIGIN      
        1 tcatcgcccc ctcgttcttg atataccgtt ccatttttcc cttcttgatc ttaaacagag
       61 gcggctgggc aatgtagatg taccggtgct ctatcagctt gggcatttgc cggtagaaaa
      121 aggtgagtag gagcgttcgg atatgggccc catccacgtc cgcatccgtc atgagtataa
      181 tcttatggta gcggagcttg ccgatgtcaa agtcctcctg gccgattccg gtgccgaggg
      241 ccgtgatcag gatccggatt tcttcactgg agatcatctt gtcaaaccgt gccttctcga
      301 cattaaggat ctttcctttg aggggaagga tcgcctggtt ccgccggtcc cgtccctgct
      361 tggccgagcc gcccgccgag tctccttcta caataaagag ttcgctcaag gccggatcct
      421 tctcggagca gtccgcaagt ttcccgggaa gcgacccgcc gtccatcgca tttttccgtc
      481 ggatcaaatc ctttgccttg cgcgccgcct cccgtgctcg cgccgcacca acggctttat
      541 ccacgatctt gcgtgcgatt ggcgggttct cttcgaaaaa ttcgctcaag gcttcgttca
      601 cagccgcctc gacgattcct ttggcctcgc tgttgcccag cttcgctttt gtttgccctt
      661 caaactgcgg gttgggcagc ttgatgctga caaccgccgt gagtccttca cggacatctt
      721 ccccagtaat agtctcgtcc ttgaggaggt tgttggccga ggcgtagcta tttacacacc
      781 gggtcagcgc ggttttaaag ccgataaggt gggttccccc ctcgcgcgtg ttgatgttgt
      841 tcgcaaaaga aaagatgttt tctgaataac cttcattgta ctgcagggcc gcttccagta
      901 tgattccgtt cttctcacgt tcgatataaa ccggcttgtg aagtgggctt ttgttttcgt
      961 taagatgctc gacaaacgag atgatgccgc ctttatagca gaacacttgc cgcttctcgg
     1021 cgcgctcatc ggccagcgtg atctcgagcc ctttatttag gaaagccaac tccctcagcc
     1081 gctgggccaa ggtgtcaaag ctgaactcca agacctcaaa gatttgtgca tcgggtttga
     1141 acgtgacctt agtgccgtgc ttcttggtct ttccggtcac ctccagggca gcctccggtt
     1201 ttccgcgttg gtatcgctgt tggtagacct gcccctcctg cttgatctcc agttcgagcc
     1261 actccgacag ggcgttcacg accgagattc cgacgccgtg gagcccgccc gagactttat
     1321 acgcctcctg atcgaatttc ccgcccgaat gaaggaccgt catcaccact tctgcggcag
     1381 atcgcttctg ggcggtatgc atcccggtcg gtatgccgcg accgtcatcg ataacggtca
     1441 cgctgttgtc gatgtgaatc gtggcatcaa tcttggttgc gtgcccgccc atcgcctcgt
     1501 ccacgctatt gtccacgacc tcgtagacaa gatggtggag cccctccggc ccggtgctac
     1561 ccacatacat ggccggccgc tttcgtaccg cttcaaggcc ttcgaggacc ttgattttat
     1621 ccgcgtcgta tcggttctct atctttgctt ccatccccta caccctcatc ggcatgacga
     1681 cgcagagata gcccggatcc ttaccctcgc gaatcagaca aggactcaag gcgtctttaa
     1741 attccaagac agcctgatca ctctcgaagg cgcccagaac atccagcagg taacgggcat
     1801 taaaccctgt ctgtagtccc tcgcctttgt aagaaaccgc catggtctcc ttcgcctcgc
     1861 ccagatcggg actgcttgag atcaaagtca accgaccagg ctccaacgag agcttgatgg
     1921 cgttggtctt ctctcttgag agcacggcaa ctcttcttaa ggccccttcg agatcctgcc
     1981 gagcaaccga aaccttttta tcattctgct taggaaccac ttgttcataa ttgggatagg
     2041 tcccttccat caatcgcgtg aacagcgtaa ttttcttctt ctggatcaga agctgatttt
     2101 taccaaagcc gagcgtcaga tcttcattat cctcctccaa gagccgtctc agctcgagca
     2161 cggccttttt gggaacgatg aggtctctgt tttcaccgtt gctcgatccc tgcggtccca
     2221 gttccctctg gatcaccgcc aatcgatggc cgtcggtccc aacgcatcgg agggcctttt
     2281 t
//
LOCUS       DATMKH010000409         1192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_513644,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000409 DATMKH010000000
VERSION     DATMKH010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513644"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..528
                     /locus_tag="VNV63_04925"
     CDS             166..528
                     /locus_tag="VNV63_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXC61995.1"
                     /translation="MNVWYSRPVLFVESVERSIAFYTEKLGFVESTRYEDEGKILVAQ
                     VNREDCEILLNCQQPEKTGRGRIFISIDLGPLQALRTEFENRGAPIRDGWWGYDTMII
                     EDPDGNELFFPYPNVAKK"
     gene            complement(550..1092)
                     /locus_tag="VNV63_04930"
     CDS             complement(550..1092)
                     /locus_tag="VNV63_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC61996.1"
                     /translation="MPPDDYFEELYGVTEERALNFWASHLHETIGNKEWSQIRDSTGV
                     YLASVLGHFCLQSVDRMELLSPDYSPSDGEFRQFADLTQIAELMMQQLTSAKNPLWME
                     SAGAHILLYAGFFQDQNKDRHNIEFYSCMGRECYLMAAVGERAGIMKLMAKGFNQYLF
                     HLANLHRHLRESRYLIRPVN"
BASE COUNT          268 a          335 c          312 g          277 t
ORIGIN      
        1 ccccacccga ttgaggaggg actcctctcc accaagaagg ccggcggatc agttcgagtg
       61 tcttccgaag gacaccgcgt cagagcctgt cctgagcgcc tgtccagcca tgacgtgttc
      121 aaccctctcc ggctatgatg atatcgccgt gcggggatcg atcgaatgaa cgtctggtat
      181 tcgcgtcctg ttctcttcgt cgaaagtgta gagcggtcga tcgcgttcta caccgaaaag
      241 cttggcttcg tcgaatcgac ccgctacgag gacgaaggga aaatcctcgt cgcccaggtt
      301 aatcgtgagg attgcgagat cctcctcaac tgccagcagc ccgaaaagac cgggcgcggg
      361 cgcatattca tctcaatcga cctagggccg cttcaagcgc tgaggaccga attcgagaac
      421 cggggcgcgc cgataaggga tggctggtgg ggctatgaca cgatgattat cgaagacccc
      481 gacggcaacg agttgttctt cccctatccg aacgttgcga aaaagtaatc aggttcccgt
      541 cagcggacgc tagttcaccg gcctgatcag atatctgctc tcccgcagat ggcgatggag
      601 gttggctaaa tggaagaggt attggttaaa gccttttgcc atcagtttca tgatcccggc
      661 cctctctccg acggccgcca tcagatagca ctctctgccc atgcatgaat agaactcaat
      721 gttgtgccgg tccttgtttt ggtcttggaa gaagccggca tacagaagaa tgtgcgcacc
      781 ggccgactcc atccacagag ggttcttcgc gctggtgagc tgctgcatca tcagttcagc
      841 gatctgcgtc agatccgcga actgtcggaa ttcgccatcg ctcgggctgt aatccggaga
      901 gagcagctcc atccgatcta ctgattgcag gcaaaaatga ccaagcacgc ttgcaagata
      961 gacacccgta gagtcacgaa tctgagacca ctccttgttt cctatcgttt cgtggagatg
     1021 tgaggcccag aagttcaggg ctcgttcttc agtaacgcca tacagctcct caaaatagtc
     1081 atcaggcggc atatccgtaa ccccgatctg ggatttgatt gcctattctt accttgtctc
     1141 agcttttagg aaatttttca ttgcacgccg catctaactg aatactctcc tg
//
LOCUS       DATMKH010000410         1058 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3696536, whole genome shotgun sequence.
ACCESSION   DATMKH010000410 DATMKH010000000
VERSION     DATMKH010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3696536"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="VNV63_04935"
     CDS             <1..283
                     /locus_tag="VNV63_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD-dependent epimerase/dehydratase"
                     /protein_id="HXC61997.1"
                     /translation="IEGIFRLAMSEHHGPVNIGNPVEMTIKQFAEKILQITGAKSVIE
                     YRDLPLDDPKVRQPDITRARTQLNNWGPKVDFEEGVRVTIDYFQKKLRD"
     gene            339..953
                     /gene="coaE"
                     /locus_tag="VNV63_04940"
     CDS             339..953
                     /gene="coaE"
                     /locus_tag="VNV63_04940"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HXC61998.1"
                     /translation="MPSIGITGGVATGKSTVSRHLLELLGRTLPVELFNSDFEARRLT
                     DSDLVVHEEIKAAFGVQVFDSKGNLARDRLRDLVFHEGAARKVLESILHPRIREAWMS
                     RTGGDGLLLAEIPLLYETGAESYFDGIIVTACSHASQVERITGERRLLEELAEQIILA
                     QMDLEEKIRRGDWVVWTDCLPGITAQQVNLISQEVIERYGGARR"
BASE COUNT          268 a          238 c          314 g          238 t
ORIGIN      
        1 gatcgaaggc atttttcgac tggcaatgag cgagcatcac gggccggtga acatcgggaa
       61 cccggtggaa atgacgatca aacaattcgc agaaaagatt ctccaaatta ccggcgccaa
      121 gtcggtcatt gagtaccgcg atctgccgct ggatgatccg aaggtgcgtc agccggatat
      181 cacgcgtgcc cgaacccagc ttaacaactg ggggccgaaa gttgattttg aagaaggggt
      241 ccgggtaacg attgattatt tccagaaaaa attgagagac taggaaggtg agttttggga
      301 ttttcccaga aactcacggc tcaaaggccg aacagcggat gcctagtatc ggaatcacag
      361 gtggtgtcgc gaccgggaag tcgacggtct cccggcacct gctcgagttg ctcgggcgga
      421 ccttaccggt tgaacttttc aactcagatt tcgaagccag gagattgacg gatagcgatt
      481 tagttgtaca tgaagagatt aaggcggcgt ttggagttca ggtattcgac tctaaaggca
      541 atctcgcacg cgatcggttg cgggacttag tattccacga aggcgcggcg cgaaaggttc
      601 tggaatccat tcttcatcct cggatacgag aggcatggat gagccggacc gggggagatg
      661 ggctgcttct tgcggaaata cctttattgt atgagacagg ggctgaatct tattttgatg
      721 gaataatcgt gactgcctgc agtcacgcct cgcaggttga acggatcaca ggcgaacgtc
      781 gattattaga agagctcgcg gaacagatca tcctagccca gatggatctc gaagagaaaa
      841 ttcgtcgggg agattgggtc gtatggaccg attgtctgcc tgggatcacc gctcaacaag
      901 ttaatctgat atctcaagag gtcatcgaac gttatggagg agccaggcgc tgaaccgacg
      961 gtgttgccgg gttcagaata ttttgacgga aacgggaatg ttcttgagct agacaggctg
     1021 caccaactta gtcaccccgt gctgctggag ctgacccg
//
LOCUS       DATMKH010000411         1537 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2758420, whole genome shotgun sequence.
ACCESSION   DATMKH010000411 DATMKH010000000
VERSION     DATMKH010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2758420"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="VNV63_04945"
     CDS             <1..620
                     /locus_tag="VNV63_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HXC61999.1"
                     /translation="VHKGNIMKFTEGSFRDWGYDLADSLFGSETYSWARWERTKKEKG
                     EKSANDEMKSALAAGKILVKDAIADITLQQILTRPEDFDVIATLNLNGDYLSDALAAQ
                     VGGIGIAPGGNINYHTGHAIFEATHGTAPKYANLDKVNPGSVVLSGEMMLRHLGWTEA
                     AELVIKGLNEAIASKRVTYDFARLMEGATEIKCSEFGDNIIDHMG"
     gene            complement(735..>1537)
                     /locus_tag="VNV63_04950"
     CDS             complement(735..>1537)
                     /locus_tag="VNV63_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020150348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HXC62000.1"
                     /translation="GGESPKGGLARYQNNPYVQQSLQYLQDQLPSLVQRTGTFIQTSL
                     TGVFGVFGFMVGLLIVPIYLFFFLKESPRIAESWSNYLPLRRSEFKDEVVSVLTEING
                     YLINFFRGQLVVAVINGILLAIGLSIVGLQFGFLIGLGFAFFGIIPYAGYIVCYIPAI
                     LIAFVQFNDWVHPLWVTVIFFLIVAQIDGLVTTPRIVGNSVGLHPMTVIMSVFLWTLV
                     LGGLLGAVLAIPLTATLKVLLHRYVWDRQRNIFFQEDISSKDEIEIAT"
BASE COUNT          418 a          389 c          429 g          301 t
ORIGIN      
        1 tcgtgcacaa gggcaacatc atgaagttca ccgagggaag cttcagagat tggggatacg
       61 atcttgccga ctcccttttt ggcagcgaga cctactcctg ggccaggtgg gagcggacaa
      121 agaaggagaa gggcgaaaaa agcgccaacg atgagatgaa gtccgccttg gcggccggca
      181 agatccttgt caaggatgcg atcgcagaca tcaccttaca acagatcctg acacgccccg
      241 aagactttga cgtgatcgca acgcttaact tgaacggtga ctatctttcc gacgctctgg
      301 cggcgcaggt cggtgggatc gggattgcgc ccggcgggaa catcaattac cacaccgggc
      361 acgcgatctt cgaagcgacc cacggcaccg cacccaaata tgcgaatctt gacaaggtca
      421 acccgggttc ggttgtcctc tcgggagaga tgatgttgag gcatcttggc tggaccgagg
      481 cagcggagtt ggtgatcaag gggctgaatg aggctatcgc gagcaagaga gtcacgtacg
      541 atttcgcgcg cttgatggaa ggggcaacgg aaatcaaatg ctcagagttt ggggataaca
      601 ttatcgacca catgggttga ggcccagtgc acgagtcggg aagtcagggg ttcaggagtt
      661 gtttctccaa attgctcctc ttgtgattgg cgttaacata ggatgattga agttagcgct
      721 tatggggctt caacctaagt cgcaatttca atctcgtcct ttgaggagat gtcttcctga
      781 aaaaagatat tccgttggcg atcccacaca taacgatgga gcagaacctt caacgtcgcg
      841 gttaacggaa tcgcgaggac cgctccaagc aggcccccaa ggacgagagt ccacaaaaag
      901 acggacatga taaccgtcat cgggtggagt ccgaccgaat ttccaacgat tcgaggagtc
      961 gtcacgagtc catcgatctg agcgacgatt agaaagaaga taaccgtcac ccacaagggg
     1021 tgcacccaat cattgaattg cacaaaggcg atcaggatcg ccggtatgta gcagacgatg
     1081 tatccggcgt aaggaataat gccgaaaaag gcgaatccga gcccgatcag aaagccgaat
     1141 tgcaggccca cgatcgaaag cccaatcgct aagaggatcc cattgataac ggcaaccacc
     1201 agttggccgc ggaaaaaatt gatgagatag ccgttgattt ccgtcaagac agagaccacc
     1261 tcgtccttga attccgagcg ccgaagcggc aggtagttgc tccaggattc cgcaatccgt
     1321 gggctctcct tcagaaagaa aaagagataa atagggacga tcagcaaccc gaccatgaat
     1381 ccgaacacgc caaacacacc ggtcaagctt gtctggataa aggtccccgt acgttgcacc
     1441 aatgaaggca actgatcctg caaatactgc agcgactgct ggacgtaggg attgttttga
     1501 tagcgcgcaa gaccaccttt tgggctctcg ccgcctt
//
LOCUS       DATMKH010000412         1315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_192966,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000412 DATMKH010000000
VERSION     DATMKH010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1315
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192966"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1315
                     /locus_tag="VNV63_04955"
     CDS             <1..>1315
                     /locus_tag="VNV63_04955"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HXC62001.1"
                     /translation="HYMRTATARVNTFYIRGEHPYPPFDQPQTLDEVEQQFKARFPTL
                     ERNAKTEEDKDLVGKLKDACAQFIQVYRQIFEYFKEGGFGNPRIGEVRQQIPSLTLRI
                     TNLSGKILDLNEQQMFKANRAAAKKSKDSIRLLMIAMISAVIVFVFTYARLGQSIITP
                     IQNLTKSIRELRSRDFAQSLTVEADDELGALTKEFNAMAEELRNFYRETDRKFIELNQ
                     VIRAMMTTLPYPLFILGARDEVSRMNPAAERLMDSLRSRGAMPKQIRKHLADASVTGP
                     DYRLDDLKQALLFRIDEQQLYFLPRIFPIVLEDGTIFGRALMLVDVTRFRWLDEMKSD
                     MLSTLSHEIRTPLTGIRLVLHLLLEKNIGELTKAQEELVITAWSDCERLLKTLNSILD
                     LARMESGKAQLDLQQVPAMRVIEESYYAFRDQAETASHPFRMDVP"
BASE COUNT          328 a          337 c          347 g          303 t
ORIGIN      
        1 gccactacat gcggactgca acagccaggg taaatacctt ttatattcgc ggcgaacatc
       61 cttaccctcc tttcgatcag ccgcagacgc tggacgaagt cgagcagcag tttaaggccc
      121 gctttccgac actcgaacgg aacgcgaaga ccgaggaaga caaagacttg gtcggaaaac
      181 tcaaggatgc ttgtgcgcaa ttcatccaag tttaccgtca aatatttgag tatttcaaag
      241 agggaggctt cggtaatccg cgtatcggtg aggtaaggca gcagatccct tcactgactc
      301 tgcgcatcac aaacctgagc gggaagattc tcgaccttaa tgagcaacag atgttcaagg
      361 caaatcgggc cgccgctaaa aagtccaagg attcgattcg attgttgatg atagccatga
      421 tcagcgccgt gatcgtcttc gtgtttacct atgcgcgcct cggtcagtcg attatcacgc
      481 cgatccagaa cttgactaaa tcgattcgag agcttcgatc tcgcgacttt gctcaatctc
      541 tgacggtgga ggctgatgat gaactcggag ccttgaccaa ggagttcaac gccatggcgg
      601 aggaactcag gaacttttac cgggaaacgg atcgaaagtt tatcgaactg aatcaggtga
      661 tccgggcgat gatgacgacg ttaccctacc ctctgttcat tctcggcgct cgagatgagg
      721 tcagccggat gaacccagcc gcggagagat tgatggattc cctgcgctct cgcggggcta
      781 tgccaaagca gatccggaaa cacttggcgg acgcatctgt gacgggtccg gattatcgct
      841 tggacgacct taagcaggcc ctgcttttcc ggatcgacga gcaacagctt tattttctac
      901 ccagaatttt tcctattgtg ctcgaagacg gcaccatttt tggaagagcc ctcatgcttg
      961 tcgacgtgac caggtttcgt tggctggatg agatgaaatc ggacatgcta tcgacgctca
     1021 gccacgaaat acggacaccg ttgactggaa tccgtcttgt gcttcacctc cttctggaaa
     1081 agaacatcgg agaattaacg aaggcccagg aggaacttgt cattaccgcc tggagcgact
     1141 gcgaacgatt gctgaagacg ctcaacagta ttctggatct tgcccgtatg gagagcggaa
     1201 aagcccagct cgatttgcaa caagtcccgg ctatgcgagt gatcgaagag agttattacg
     1261 cattccgtga ccaggccgag acggcttcac atccctttcg gatggatgtt ccgga
//
LOCUS       DATMKH010000413         1113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4361878, whole genome shotgun sequence.
ACCESSION   DATMKH010000413 DATMKH010000000
VERSION     DATMKH010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4361878"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..309)
                     /locus_tag="VNV63_04960"
     CDS             complement(139..309)
                     /locus_tag="VNV63_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62002.1"
                     /translation="MGFWALFPRHHPITFETYVDEREFTCARIGGVVIPLLRFYLQND
                     PPAHDVGDTGEF"
     gene            354..623
                     /locus_tag="VNV63_04965"
     CDS             354..623
                     /locus_tag="VNV63_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62003.1"
                     /translation="MNIFKTVAKKIGSILLNFAYNEDRSIASLGGAPKDFTISAESAK
                     QPFLKPLGAALDDVHFAGDPNHAQDALIHDTALQAADNQLKAEGK"
     gene            620..>1113
                     /locus_tag="VNV63_04970"
     CDS             620..>1113
                     /locus_tag="VNV63_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62004.1"
                     /translation="MKTPTIAVFGDNFIDRYYIGTATRLSPEAPIPVVNIQEEKVFPG
                     GAGNVVANLDTLGAKILGVEYGRVPIKNRLYSDNHQIARWDENDESTELEIEYLHQLR
                     ADAVIISDYGKGSITYAVIEAIAALNLPTFIDSKRSPRDFDIVMNPTFFPNQKEYNAH
                     IQDYR"
BASE COUNT          306 a          310 c          243 g          254 t
ORIGIN      
        1 tggatattgt cctcatgaaa agaaaggtgc ggtagcagca tagccactcg ttggattcaa
       61 ttcgacgcta atagtctttt gaagctcctt tgccactata cgatggatca taatccacag
      121 atccctcttg acaccgaact aaaattcgcc ggtgtctcca acatcatggg ccggtggatc
      181 gttctggagg tagaagcgga ggagtgggat cacgacaccc ccgatcctcg cacaggtgaa
      241 ctccctttca tccacatacg tttcgaaggt gatcgggtga tgtcgtggaa acaaggccca
      301 gaaacccaca ctaaagactc ttggaaagat gctgtagatt cccccggatc attatgaaca
      361 tatttaaaac cgtcgcaaag aaaatcggtt ccatcctcct gaacttcgct tacaacgaag
      421 accgttccat cgcatccctc ggaggtgccc cgaaggattt cactatctcc gcggaaagcg
      481 cgaaacaacc atttctaaaa cccctcggag ccgctttgga tgatgtccac ttcgcgggcg
      541 accctaacca cgcacaggat gcattgatcc acgacaccgc tctgcaagcc gcggacaatc
      601 aactaaaggc cgaaggaaag tgaaaactcc caccattgct gtgttcggtg ataacttcat
      661 cgatcgatac tacatcggca cagccacccg cctatctccg gaagctccaa tcccggttgt
      721 gaatattcaa gaagagaaag tctttcctgg tggtgctggg aatgtagttg ccaatcttga
      781 tactcttggt gcaaagattc ttggagttga atatgggcga gtcccgatca aaaaccgtct
      841 ctactctgac aatcaccaaa tcgctcgttg ggatgagaac gatgaaagta cggaactgga
      901 gatcgaatac ctccatcaac tccgagccga cgctgttatc atctccgact atggcaaggg
      961 ttccatcacc tacgcagtca tcgaagcgat cgcagctcta aacctcccca ccttcatcga
     1021 ctcgaaacgt tccccacggg atttcgatat cgttatgaat cccaccttct tcccaaatca
     1081 aaaggaatac aacgcacaca ttcaggatta ccg
//
LOCUS       DATMKH010000414         1762 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_832206,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000414 DATMKH010000000
VERSION     DATMKH010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_832206"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..826)
                     /locus_tag="VNV63_04975"
     CDS             complement(<1..826)
                     /locus_tag="VNV63_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62005.1"
                     /translation="MPKPLLIISDAITSPSGLGRVTRELAIRIHKDLSDVFRLGVAGY
                     GGTYSRKFPFPQYTFAKLENWAIQELPGIWYDFAGEEKGIVFFIWNASWLTWAADPDT
                     LQDGDLKTVLKSGAFKKWIYAPVDSVSPNDRLHQSEKMILSKFDRVLAYTKWGAAVID
                     RTFHSPVSVHPEFPVTDFLPHGTDGSIFFPRDRGEARRTFVERIVKRGAGRISDDVVF
                     LGVCATNTPRKDWGLAFETCGELLNLGVNVGLWAHTDRFQKVGAWDLLNLADEFGMR"
     gene            complement(846..1196)
                     /locus_tag="VNV63_04980"
     CDS             complement(846..1196)
                     /locus_tag="VNV63_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015891832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62006.1"
                     /translation="MIHAAKKQFKFDESYREYYMELAHRFREKHFPMYALDGTYGRAL
                     CIVDLVDCVPTASLRGRIGRTEFWGDFRDVGDDGKSRWAFKLENVRRLWPRPLVIGRQ
                     RFFEIHDALLKFEK"
     gene            complement(1295..1672)
                     /locus_tag="VNV63_04985"
     CDS             complement(1295..1672)
                     /locus_tag="VNV63_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62007.1"
                     /translation="MKITNQELAELFKSALVAYENEPDKGVGPDGYCYDTLAGGRVAI
                     EMAAKILLQPPQDDENEVAFDTSSFLRRRLKSVMPKDRAVEAVQRWLLKRAWTANSDN
                     VDLAELIVGAVRMADKGFREVLK"
BASE COUNT          422 a          542 c          391 g          407 t
ORIGIN      
        1 gccgcatacc gaactcgtcg gccaagttca gcaagtccca agccccgacc ttctgaaacc
       61 tgtccgtgtg agcccataaa ccgacattta cccccagatt tagcaactca ccacaagtct
      121 cgaaagccaa accccaatcc ttccgcggcg tattcgtagc acacacacca agaaacacga
      181 catcatctga aatccgcccc gcccccctct tcacgatgcg ctccacaaac gtcctacggg
      241 cctcgccacg gtctcgcggg aagaaaatgg acccatcggt cccgtgaggc aagaaatccg
      301 tcacagggaa ctccggatgc acggaaacag ggctatggaa ggtcctgtca attactgcgg
      361 ctccccactt ggtataggcc aacactcggt cgaatttgct tagaatcatt ttttccgact
      421 ggtgcagcct gtcgttcggg ctgactgaat cgaccggagc gtaaatccac ttcttgaatg
      481 ctccactctt gagaacggtt ttcaggtcgc catcctgaag ggtatccgga tcagccgccc
      541 acgtgagcca gcttgcattc cagatgaaga atacgattcc cttctcttct ccggcaaagt
      601 cgtaccagat ccccggcaac tcctggatgg cccagttctc aagcttcgcg aacgtgtatt
      661 gagggaaagg gaatttcctt gagtacgttc cgccatatcc ggcaactccc agacgaaaga
      721 catcactcag atccttgtgg atgcggattg caagctccct cgtaacgcgg ccgagtccgc
      781 tgggggacgt aatggcatcc gagatgatca ataaaggctt aggcactctt actccaaaat
      841 ttggactact tttcgaactt cagaagcgcg tcgtggatct caaaaaaccg ctgcctcccg
      901 ataactaatg gccggggcca aagtcttcgt acattctcca gcttaaaagc ccaccgggat
      961 tttccatcgt cgccaacgtc tctgaaatct ccccagaact ccgtccgtcc gattcttcca
     1021 cgaagactcg cggttggtac gcagtcaacc agatcgacaa tgcaaagagc ccttccatat
     1081 gtgccgtcga gagcatacat cgggaagtgt ttctcccgaa agcgatgagc caattccatg
     1141 taatactccc ggtagctctc gtcaaacttg aattgtttct tcgcggcatg gatcaccagg
     1201 ggaccccgat aagacgtcgc ccatggcctc gtctcatatg gcttcaggcc aagctctatt
     1261 gcgctcgcgt gtggctgcca aagactcaga gccttcattt caagacctct cggaatcctt
     1321 tatctgccat acggactgca ccaacgatca actccgccaa atccacattg tcagagtttg
     1381 cagtccaagc tcgcttcaac aaccatcgct gcactgcctc aaccgcccgg tctttcggca
     1441 taacgctctt tagtctccgg cgaagaaacg aactagtgtc aaatgcaact tcattttcat
     1501 catcttgggg aggttgaaga aggatcttag ccgccatctc aatcgccacc cggcccccgg
     1561 caagagtgtc gtagcagtac ccgtctgggc ctacaccctt atcaggctcg ttttcgtaag
     1621 caaccagggc actcttgaac agttccgcca actcttggtt cgtaatcttc atgttggcag
     1681 tttctcacaa atacttggtt tcgtggtttt aacgatcaat ctcgtatcct ggagggcaaa
     1741 gttcccgtac agcaccctcc at
//
LOCUS       DATMKH010000415         1026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4670630, whole genome shotgun sequence.
ACCESSION   DATMKH010000415 DATMKH010000000
VERSION     DATMKH010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4670630"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="VNV63_04990"
     CDS             <1..231
                     /locus_tag="VNV63_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62008.1"
                     /translation="TGNLFGFPEKESNDVIAEAFRKRLKEVAGFTRVPAPLPMRNSKG
                     ATVYYLFFASQKDTAEHIVLQIFDKFRSRGEQ"
     gene            231..>1026
                     /locus_tag="VNV63_04995"
     CDS             231..>1026
                     /locus_tag="VNV63_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002622119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage Gp37/Gp68 family protein"
                     /protein_id="HXC62009.1"
                     /translation="MSLQSSIEWTDATWNPVRGCTKISPGCKHCYAETFAERFRGVKG
                     HPYEQGFDLRLVPGKLGEPLRWRTPKMIFVNSMSDLFHDQVPDEYIVAVAKVMTAAKW
                     HTFQVLTKRSERIAGLLGTKLAFASLQPHIWWGVSVEDRKYGLPRVGHLRAAPAAVKF
                     LSVEPLLEDLGRVNLNGIDWVIVGGESGPGARPMEKKWVLSLRDQCREASVPFFFKQW
                     GGVRKSTTGRRLEGKTYDELPRRISQPILDSESCVAIAAEIEGSCKV"
BASE COUNT          241 a          269 c          289 g          227 t
ORIGIN      
        1 actgggaatc tcttcggatt tcctgagaag gaatcaaatg atgtgatcgc agaggctttc
       61 cgaaaaagac ttaaggaagt agccggattt acgcgtgtcc cagcacctct gccaatgagg
      121 aactcaaagg gagcgacggt atactatctt ttctttgctt ctcaaaaaga cacagctgaa
      181 cacatagttc ttcagatctt tgataagttt agaagccgag gggaacagtg atgtcgttac
      241 aatcctccat cgagtggacc gatgccacct ggaacccggt ccggggctgc accaagataa
      301 gccctgggtg caaacattgc tacgccgaga cgttcgccga gcgctttcgc ggcgtcaaag
      361 gccatcccta tgagcaagga tttgatcttc gcctcgttcc gggaaaactc ggggagccgc
      421 tgcgctggcg cactccgaag atgatctttg tcaattccat gagcgatctc ttccacgacc
      481 aggttcccga cgaatacatt gtcgccgtcg cgaaggtcat gaccgccgcg aagtggcaca
      541 cctttcaagt cctgacaaaa aggtcggagc gcattgccgg gctccttggg accaagcttg
      601 ctttcgcctc tctgcaaccc cacatctggt ggggcgtgag cgtggaagac aggaagtatg
      661 gtcttccacg cgtcggccat ctgcgcgcgg ctcctgcagc tgtgaaattt ctctccgttg
      721 agccgctact ggaagatctc ggccgggtga atcttaacgg aatagactgg gtcatcgtcg
      781 gtggtgaaag cggccccggt gccagaccaa tggaaaaaaa gtgggttctg tcgcttagag
      841 accagtgccg ggaagcatcg gtgccgttct tctttaagca gtggggaggc gtccgcaaat
      901 ccacgaccgg ccgaaggctg gagggcaaaa cctacgatga gcttcctcgg aggatttctc
      961 aaccaattct cgattcggaa agttgtgtgg cgatcgccgc agagattgaa ggttcctgca
     1021 aagtaa
//
LOCUS       DATMKH010000416         3374 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2429469, whole genome shotgun sequence.
ACCESSION   DATMKH010000416 DATMKH010000000
VERSION     DATMKH010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2429469"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="VNV63_05000"
     CDS             complement(<1..286)
                     /locus_tag="VNV63_05000"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HXC62010.1"
                     /translation="MAKENLSKLDTGVTVDLTIDGEKVTAPEGRSLYDVLSGIGKIIP
                     AMCYHYTFTPFGSCGMCLVEVEGKKAPVRSCTAPVQASMVVRTETPELKAA"
     gene            complement(390..1700)
                     /gene="nuoD"
                     /locus_tag="VNV63_05005"
     CDS             complement(390..1700)
                     /gene="nuoD"
                     /locus_tag="VNV63_05005"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HXC62011.1"
                     /translation="MEINEKTITETKPADPVQTQTGSQPVGRLSDVQGEAGLKDGKRG
                     LPLLRTEKLMLNMGPQHPSTHGVLKVLLDLEGERIVKATPVMGYLHRGVEKLAEDGTY
                     HQFIPHTDRLDYVCAMYNNFAYVRAVEKLLDLKVPERAEYLRTIVAEVQRIIGHLFWL
                     GSQALDIGAMTVFFYTFREREVLLDFFDDLCGARLTTSWYRIGGVEGDFTQKQIDVLY
                     KFLSNFSEKLDEYNILLETNRIWLARTKGVAVISAEDAISFGLSGPSLRGSGVDYDLR
                     KYEPYAAYDKVQWEVPIGKNGDTYDRYWIRILEMKESAKIVKQCLDQLPSGPIIADAP
                     KIVLPTKERVFKNLEHMIIQFRLFTHGFRPPVGDIYCGTEAHKGELGFYIVSDGTGKP
                     YRLKIRAPSFIHLGAFDHMARGYMIADVVTIFGTYDVVMGECDR"
     gene            complement(1768..2280)
                     /locus_tag="VNV63_05010"
     CDS             complement(1768..2280)
                     /locus_tag="VNV63_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HXC62012.1"
                     /translation="MHPLAEKTQARFPEAFLSAKEFAGDLSVQIKKEGLVEICRYLHD
                     DPEMDFDYIIHISSVDWPNDEERFEMVYEFYSIRRRQRIRLKARVTEEDCSIDSVTGI
                     WKGADFMEREVYDMMGIRFNNHPDLRRILMPDEYEEGYPLRKDFPLVGRGWRDTFEFL
                     PSPSGGEKKS"
     gene            complement(2280..2774)
                     /locus_tag="VNV63_05015"
     CDS             complement(2280..2774)
                     /locus_tag="VNV63_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HXC62013.1"
                     /translation="MGLDHAKDGDTGILTLTIEQAVNWARKGSLWPLTFGLACCAIEM
                     MTAVSPRYDIDRYGAGVFRASPRQSDLMIVAGTVCRRMAPVIRKIYDQMPEPKYVISM
                     GSCATSGNIYNSYPVVQGVDRFVPVDIYVPGCPPTPEALFDGILKLQEKIMKNRVFLE
                     RMKV"
     gene            complement(2765..3148)
                     /gene="ndhC"
                     /locus_tag="VNV63_05020"
     CDS             complement(2765..3148)
                     /gene="ndhC"
                     /locus_tag="VNV63_05020"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HXC62014.1"
                     /translation="MDKAAYFSAHLFQYFPVVLFIGATVAFGIAILVISYYVQPRIPQ
                     KEKLEAYECGADPISDARMPFPVRYYIIAMLFVIFDIEVIFLYPWAVVFPKVKFLGFI
                     EMLVFIALFLAGYVYAWKKGALEWD"
BASE COUNT          798 a          994 c          801 g          781 t
ORIGIN      
        1 gcgccgcctt aagttccggc gtctcggtcc ggaccaccat gctagcctga accggtgcgg
       61 tacaggacct caccggcgcc tttttgcctt ccacctcgac gagacacata ccgcaggagc
      121 cgaacggcgt aaaagtgtag tgatagcaca tggccggaat aatcttgccg atgccgctga
      181 ggacatcata tagggaccgg ccttccggtg cggtgacttt ctcaccgtcg atggtcagat
      241 caaccgtgac accggtatcc agtttgctta ggttttcttt tgccattatc tatcctttat
      301 acctttatat gtgggggccc gtgcggtaca gtagctcctc cgatgccccc acaccccgcg
      361 gcagccacag gcaaagcctg atgactgcct tatctatcgc attcccccat gacaacatcg
      421 tacgttccaa agatcgtgac gacgtccgcg atcatgtagc cgcgcgccat atgatcgaag
      481 gcacctagat ggatgaacga cggcgcccgg atcttcaagc gatacggttt tccggtccca
      541 tcgctcacaa tgtagaaacc aagttcaccc ttgtgcgcct cggtcccgca gtagatgtcg
      601 cccaccggtg gccggaagcc atgtgtgaag agccggaact gaataatcat gtgttcgagg
      661 ttcttaaaga cccgctcttt cgtaggcaag acgattttgg gcgcatccgc aatgattggt
      721 ccggagggaa gctgatccaa acattgcttg acgatcttgg cgctttcctt catctccaga
      781 atccgtatcc agtagcgatc atacgtgtcg ccgttcttgc cgatcggcac ttcccactgg
      841 accttatcgt aggccgcata cggctcgtat ttgcggagat cataatccac acccgacccg
      901 cgaagtgacg gcccgctcaa gccgaagctg atggcatctt ccgccgagat aaccgcaacc
      961 cccttggttc gtgcaagcca gatacggttt gtctcgagca ggatattata ctcatccagc
     1021 ttctcagaaa aattactcaa aaacttataa agaacatcga tctgtttctg tgtaaaatcc
     1081 ccttccacac ctccgatccg ataccaactc gtcgtaagcc gggccccgca gaggtcatcg
     1141 aagaaatcca gcagcacctc gcgttctctg aaggtataaa aaaagacagt catcgcgccg
     1201 atgtccagtg cctggcttcc cagccagaag agatgcccga taatccgctg cacctcagcc
     1261 acgatcgtcc ggagatactc tgcacgctcc ggaaccttaa gatccagaag cttctcgacc
     1321 gccctgacat aggcaaagtt gttgtacatc gcacagacgt aatccaatcg atccgtatgc
     1381 gggatgaact gatgatacgt cccgtcctct gccagcttct ccaccccgcg atgaaggtac
     1441 cccatgaccg gtgtcgcttt cacgatccgc tctccttcca ggtccaggag caccttgaga
     1501 acgccatgcg tgctgggatg ctgaggcccc atattgagca tcagcttctc ggtccgaaga
     1561 agcgggagcc ctcgctttcc gtccttcaga cccgcttctc cctgcacgtc ggacagacgg
     1621 cccacaggct ggctgccggt ctgggtttgg accggatcag ccggttttgt ctctgtgatt
     1681 gttttctcgt ttatttccat tttattcttt cattcttacc agcttcgcgc ttaccagatt
     1741 cgtgcttgtc ggtttagagc gaatacctta acttttcttt tccccgccgc tcggtgatgg
     1801 aaggaactca aaagtgtccc gccatcccct cccgactaac gggaagtctt ttcgaagcgg
     1861 gtaaccctcc tcatattcat ccggcatgag gatgcgccga agatcgggat gattgttgaa
     1921 ccggatcccc atcatgtcgt acacctcccg ttccatgaag tccgcccctt tccagattcc
     1981 agtcacggaa tcgatcgagc agtcttcttc ggttacacgg gctttcaagc ggatccgctg
     2041 ccgccgccgg atcgaataaa attcgtatac catctcgaac cgctcctcat cgttcggcca
     2101 gtccaccgag ctgatgtgga tgatatagtc aaaatccatt tccggatcgt catgaagata
     2161 gcggcaaatc tcgaccaggc cttctttctt aatctgaacc gaaagatcgc ctgcaaactc
     2221 cttcgcactc aggaaggcct ctggaaatcg ggcttgggtc ttttcagcca acggatgcat
     2281 cagaccttca tcctctccag gaagaccctg ttcttcataa tcttctcttg tagtttcagt
     2341 atgccgtcaa acagggcctc tggcgtcggc gggcaaccgg gcacatagat gtccaccggg
     2401 acaaagcggt ccacaccctg aaccacggga taactgttat agatgtttcc ggaggtggcg
     2461 caggagccca tcgagatcac gtattttggc tccggcattt gatcatagat ctttcggatg
     2521 accggggcca tgcggcggca aaccgtcccg gccacaatca tgagatccga ttggcgcggc
     2581 gaggcacgaa acactccggc cccgtagcga tcgatatcat accgtggaga aaccgccgtc
     2641 atcatctcaa tcgcacagca ggcaagaccg aacgtcagag gccagagaga gcctttgcgg
     2701 gcccagttga ccgcctgctc gatcgtcagt gtgagaattc ccgtatcccc gtcttttgca
     2761 tggtctagtc ccattctaaa gctcccttct tccaagcgta cacatagccc gccaagaaca
     2821 gggcgataaa gaccaacatc tcaatgaagc ccaagaactt gaccttcgga aaaaccaccg
     2881 cccagggata tagaaatatc acctcgatat cgaagatcac aaagagcatg gcgatgatgt
     2941 agtatcgcac cggaaacggc attcgggcat ccgagatcgg atcggctccg cattcgtagg
     3001 cttccagctt ctctttttgg ggtatacgag gttgaacgta atagctgatg accaagatcg
     3061 cgatcccaaa agcaaccgtt gccccaataa acagaacgac tggaaagtat tgaaaaagat
     3121 gcgctgaaaa ataagccgct ttgtccatca atttcctctg cgtttgaggt tgattattct
     3181 cgcattttcc gggcttgcag tcaagggtcc agaagcccta ccctgaagcc caaaaggcct
     3241 aagacaggtt caccgaaacc attgatattt agatatatta aaagaaagat ccacctggag
     3301 aaaaggcctc tttgccccta cagaagagag acaatcaaca gcgccaccaa attgatcacc
     3361 ttgatcatcg gatt
//
LOCUS       DATMKH010000417         2750 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1157966, whole genome shotgun sequence.
ACCESSION   DATMKH010000417 DATMKH010000000
VERSION     DATMKH010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1157966"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="VNV63_05025"
     CDS             <1..514
                     /locus_tag="VNV63_05025"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HXC62015.1"
                     /translation="LFGVLPQAGLLCVMEALEKEVKLNESSLESLYSLSWEMLSEMHR
                     AGITIGSHTKTHAILTKESQPKVLEELTGSRQQLEKRLGVAIQHIAYTDGQFDTKTLS
                     AVAASGYRFGYTTCHHRNPRYPVLTIPRHILWETSCMDVLGRFSSSMMNCHVNGVFKL
                     IDRCRDNHGT"
     gene            639..2552
                     /gene="asnB"
                     /locus_tag="VNV63_05030"
     CDS             639..2552
                     /gene="asnB"
                     /locus_tag="VNV63_05030"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HXC62016.1"
                     /translation="MCGIAGILDFNGGQVSLEELHSMCDAIIHRGPDDQGVYLDRGVG
                     IGMRRLSIIDLQTGQQPVRNEDGSVWVVFNGEIYNYKDLRRELEGKGHSFYTETDTET
                     IVHLYEEYGVRCMEKLRGMFTFAIWDKKRRQMLLARDRLGIKPLYYGVIAGRLVFASE
                     LKAILQLPDIESRLNWGAVDHLFSFLSTPRSDSIIEGMRKLEPGHFLVASPGKEVSIE
                     RYWDVSFEPDYSHDERYFVERLRELLTESVRSHLVSDVPLGAFLSGGIDSSSITAAMS
                     KLTAGPVKTFSIGFPDQDYNELKHARLVAKHLGTDHHELVLEPNVLDILEDLTWHLDE
                     PFGDSSAIPTFMVSKMAAEHVTVVLSGDGGDELFAGYDKYVVEEREKRKYGFLPRPVR
                     KMLGTVGRMIPEGVRGHNFLNHLSLPDLERYLDSCTLFRNEQKKKLFRAEVFDLFSTK
                     DRRGAGYFSRVNGHWLSTMQYWDLNDYLPLDILTKVDRMSMAHSIEVRVPLLDHKLVE
                     FAATIPPELKLRNGTTKYIFKRAMRGILPDEVIDRPKQGFAIPLSRWFRGRLNGFIRE
                     LLLSDTVRRRGVFNVEYIEQLLKWHEKGRNLDLQLWTLISFELWCRIFLDRRSSKTTS
                     NGFKNSQSFVVTK"
     gene            2566..>2750
                     /locus_tag="VNV63_05035"
     CDS             2566..>2750
                     /locus_tag="VNV63_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62017.1"
                     /translation="MKSLKLIATGWMLAIILGLIGAFWTPSVMGQGKSGKFGQGPDIG
                     SQAGLRATDAQWPLSFEA"
BASE COUNT          671 a          650 c          765 g          664 t
ORIGIN      
        1 gttattcggc gttctacccc aggccggact tctctgcgtc atggaggccc ttgaaaagga
       61 agtcaaactg aatgaaagca gtctggaatc gctttattct ttaagctggg agatgctctc
      121 tgaaatgcat cgtgcaggaa ttactatcgg ttcgcataca aagacccatg ccatactgac
      181 caaggaaagt cagccgaagg tattggaaga gctcacgggc tcgcggcagc aactggaaaa
      241 gaggcttgga gttgcaatcc agcacattgc ttatactgat ggacagttcg atacgaaaac
      301 cttgagcgcc gtggcagctt ctggttatcg ctttggctac accacttgtc atcaccgtaa
      361 tccgcgttac cccgtattga cgatcccacg ccatattctt tgggaaacct cctgtatgga
      421 tgtactcggc cggttctcgt catccatgat gaactgccac gtaaacggcg tcttcaaatt
      481 gattgaccgt tgtagagata atcacggaac ttgaaggaac cttcgaaaat ggattggtaa
      541 gcgacattga gaagcttgag attatagact cgcttcagag gtttattgcg ggtacgttca
      601 agatgggaac ccttaaaagg agttagaggc agccgagaat gtgtggtatt gctggaatct
      661 tagatttcaa cggagggcaa gtatccttgg aggagctgca ttccatgtgc gatgcgataa
      721 tacatcgcgg tccggacgat caaggtgtct atctggatcg gggcgtgggc attggaatgc
      781 gtcgcctaag cattatagat ctgcagaccg gacagcagcc cgttcgaaat gaggacggtt
      841 cggtttgggt ggtttttaac ggagaaattt ataactacaa ggacctacgg cgcgaactgg
      901 aagggaaagg acattctttt tacacggaaa ccgataccga aacgatcgtc cacctgtatg
      961 aagagtacgg ggtccggtgt atggagaagc tacgtggaat gttcaccttt gcgatctggg
     1021 ataagaagcg gagacagatg cttctggcgc gcgatcgtct gggaatcaaa ccgctgtatt
     1081 atggtgtgat tgcggggcgc ctggtgttcg catcggaact gaaggcgatt ctccagttgc
     1141 ccgacattga gagtcggctc aactggggtg cggtcgacca ccttttcagt ttcctttcaa
     1201 ccccgcgatc agacagcatc atcgaaggaa tgcggaaact cgagcccggc catttcttag
     1261 tggcctcgcc cggaaaagaa gtcagcatag agaggtattg ggatgtctcg ttcgaaccag
     1321 actacagcca cgatgaaagg tacttcgtgg agcggctccg ggagcttctt acagaatcgg
     1381 tccgctcgca cctcgtcagc gatgtccctt taggtgcctt cctaagcggt gggatcgatt
     1441 caagctctat cacggccgcc atgtcgaaac tgaccgcggg tccagtcaag acattttcca
     1501 ttgggttccc cgatcaggat tataacgagc tcaaacatgc cagattggtg gcaaaacacc
     1561 tcggcacgga ccatcatgag ttagtcctcg agcccaatgt gctggacatt ttagaggacc
     1621 tcacctggca tctggatgaa ccgttcggcg actcttccgc catcccgacc tttatggtat
     1681 cgaagatggc cgccgagcat gtgaccgtag tgctctccgg cgatggcggc gatgagcttt
     1741 tcgcgggata cgacaagtac gtcgttgaag aaagagagaa aaggaaatac gggttcctgc
     1801 cgaggccggt tcggaagatg ctcgggacag tcggccgaat gatcccggaa ggagtgcggg
     1861 ggcataactt cttaaatcat ctttcgcttc ccgacctgga gcgttatctg gattcctgca
     1921 cgctttttag gaatgagcag aaaaagaagc tttttcgggc tgaggtcttc gatttatttt
     1981 cgactaaaga tcgccggggt gctggatact tttcacgggt gaacggccat tggctttcga
     2041 cgatgcaata ttgggacctc aacgattatc ttcctctgga tatcctgacc aaggtggacc
     2101 ggatgagtat ggcgcattcc atcgaggttc gtgttccgct gctggatcac aaactggtcg
     2161 aattcgccgc caccattcct ccggaactca agttgcgcaa tggtacgacg aaatacatct
     2221 tcaaacgcgc catgagggga atcctgccgg atgaggtgat cgaccggccc aagcaaggtt
     2281 ttgcaatacc gctgtcccgc tggtttagag gacggctcaa tggttttatc cgtgaactgt
     2341 tgctctcaga cacagtccgg cgccgcgggg tttttaacgt tgaatatatc gagcagctct
     2401 tgaagtggca cgagaagggt cgaaatctgg atctgcaact ctggaccctc atttctttcg
     2461 agctttggtg ccggattttt ctggatcgaa gatcaagcaa aacgacctca aacggtttta
     2521 aaaacagcca atcgttcgtt gttacaaagt gaaaaaaagg aagtcatgaa gtcgctcaaa
     2581 ctaattgcca cgggatggat gctggcgata atcttagggc tgattggagc attctggact
     2641 ccgagtgtta tgggtcaagg gaagtccgga aagttcggtc agggaccgga tatcggcagt
     2701 caagccgggc tcagagcgac cgatgctcaa tggcctctca gcttcgaggc
//
LOCUS       DATMKH010000418         1109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_834446,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000418 DATMKH010000000
VERSION     DATMKH010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834446"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="VNV63_05040"
     CDS             complement(<1..446)
                     /locus_tag="VNV63_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62018.1"
                     /translation="MKMMRSLGISLVMILLSAPFVRGQDLSKYRDFSLGMSLPELSSQ
                     VDLRPLQTKLIQKHPAVIQELTCWPGGSSDYSRQTDSVGQIFFSFHNGELFRILVTYD
                     HAATHGLTAEDMVQAISTKYGNPTRPVGEISFPTNELYRSTEKVIAR"
     gene            complement(560..997)
                     /locus_tag="VNV63_05045"
     CDS             complement(560..997)
                     /locus_tag="VNV63_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62019.1"
                     /translation="MADAIKMGTILIREGTLLPAGVPVESDVFLPGWRLVKNLEREGL
                     DQKFHEAGCTFLSVAGEIGATVFGLDEQKTLRRAVEQILAKRGSAEFNSVEIMRVAWE
                     TSKRFLGVSCVTVSAQSRDIQEGASLSRAKDLPVEERARLAAA"
BASE COUNT          255 a          317 c          265 g          272 t
ORIGIN      
        1 cgggcgatga ccttttctgt cgacctatac aattcgttag ttgggaagct gatctcgcca
       61 acaggcctcg ttggatttcc gtactttgtc gagatggctt gtaccatgtc ctcggcagtt
      121 agcccatgtg ttgcggcgtg atcataagtc accaaaatcc tgaagagctc gccattgtgg
      181 aagctgaaga aaatctgccc gaccgagtct gtttggcgcg aatagtcaga ggagccgccc
      241 ggccaacacg tcaactcttg aattaccgct ggatgctttt ggatcagctt tgtctggagc
      301 ggtcttaggt caacctggct agatagttcg ggcaaactca tccccaacga aaagtctcga
      361 tacttggaaa gatcctgccc gcgaacgaaa ggagctgaaa gcaggatcat aacgagggaa
      421 atgcctagac tgcgcatcat tttcatttga acgcttccta cgaaacctta aagactcaaa
      481 atcatctcca ctttagccag tgcgatcatt tctccctgac gcaattcgtc gttgaccctt
      541 gcgagtccca tcctttggtt caagcggcag ccaatcgtgc gcgctcctcc accggaaggt
      601 ccttggcccg ggaaagagac gcaccttcct ggatatctcg tgactgggcg gagacagtca
      661 cacagctcac ccccaggaaa cgtttcgagg tctcccaggc cactcgcatg atttccacgg
      721 agttaaattc cgctgacccc cttttcgcca ggatttgctc gactgctctt cggagcgtct
      781 tttgttcgtc aagaccgaag acggtcgccc caatttctcc agccaccgag aggaaggtac
      841 agcccgcctc gtggaatttt tgatccaatc cctctctttc gagattcttg actaatctcc
      901 agcccggcaa gaagacatca ctttctacag gcacacctgc cggtaagagt gtgccctccc
      961 ggatcaggat ggtccccatc ttgattgcgt ctgccatttg actttctatt ccatcaacag
     1021 tgttacagca cgcggaagta actgtgaccg gcggtcaatc ggcgagccaa tcttcatacc
     1081 cgatggatcg tcggactacg agacttaaa
//
LOCUS       DATMKH010000419         1306 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2430221, whole genome shotgun sequence.
ACCESSION   DATMKH010000419 DATMKH010000000
VERSION     DATMKH010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2430221"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            139..1104
                     /locus_tag="VNV63_05050"
     CDS             139..1104
                     /locus_tag="VNV63_05050"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HXC62020.1"
                     /translation="MFDQFLSKAGELMSAESPFAVAIVIRHEPPVSGKPGDKAIIRSD
                     GQVWGWIGGGCVQPLVIREALNAMEEGSPRLVRISPSAESDPEIGTVHYPMSCHGGGA
                     LEVYIEPVLPKPRILIFGRSPAAQSLAKLGKAVGYGIGVVAPKAGRENFPDADFIGEK
                     FLPSQIKNASETYIVVATQGEHDEEALEHALSTNARYISFIASSAKAQKIIGFLAERG
                     ISQEMLRRIKAPAGLQIGTSSSQEIAVSILAEIIQVKKGRPLQVTPQEASDLGTPLEI
                     TRDPVCGMTVDPAESTLKSDYKGRLFHFCCAGCQQTFEREPEKYV"
BASE COUNT          307 a          349 c          367 g          283 t
ORIGIN      
        1 ggaatgagtg cggcgctgcc tttcatcgac gtgtttgcgc cggcccataa cttgcaaagc
       61 ctgctcgcgc tggagcaaca cctggacccg aaaatctcgc cgggcaaagc gtttccggga
      121 ttttcgggct gaagaccgat gtttgaccaa ttcttgagca aagcgggtga gttaatgtca
      181 gcggaatctc ctttcgcggt ggcgatcgtc atccggcatg aaccgccggt ttcgggcaag
      241 ccgggcgaca aagcgatcat tcgatcggat ggccaggtct ggggctggat aggaggcgga
      301 tgcgtgcaac ccttggtgat tcgggaggcc ttgaacgcga tggaggaggg gagcccgcgg
      361 cttgttcgaa tctcgccctc cgcagagtct gacccggaaa tcggaaccgt gcactatccg
      421 atgagctgtc atggaggggg tgctctggaa gtttacatcg agcccgtctt gccaaaaccg
      481 cgaatcctga tctttggccg ctcaccagcc gctcaaagtt tagcgaagct cgggaaagct
      541 gtcggctacg ggatcggcgt cgttgctccc aaagcaggtc gggagaattt tccggatgcc
      601 gattttattg gcgaaaaatt tcttcccagc cagatcaaaa atgcatctga gacttacatc
      661 gtcgttgcca cgcagggaga gcacgatgaa gaggcgctgg aacacgcgct cagtaccaac
      721 gcccgctaca tctccttcat cgcgagctct gccaaggccc aaaagatcat cggtttcctg
      781 gcggagaggg ggatatccca ggaaatgctg cgtcggatca aagcgccggc cgggctgcaa
      841 atcggaacct catcctcgca ggaaattgcg gtgagcattc tagctgaaat tattcaggta
      901 aaaaaaggtc gcccgctaca agtcaccccg caagaggcgt ctgacctcgg tactcctctg
      961 gagatcacga gagatcccgt ctgcggcatg acggtcgatc cggccgaatc aaccctgaag
     1021 tcagattata agggaagact atttcacttc tgctgcgccg gctgccaaca gacatttgaa
     1081 agggaaccag agaaatatgt ttaggggaag aatcctgaat tcagaattca ggagttgcag
     1141 gagttcagga gttgcagaat aaggaaattc agttttgctc ctaagaatct taagaggaag
     1201 cttgacctct gacgcccact tttttcaccg cgaatcgatg actcctgact cctgactcct
     1261 gactcctgac tcctgactcc tgaattcccg tgatcaccgc tctcat
//
LOCUS       DATMKH010000420         2647 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4028904, whole genome shotgun sequence.
ACCESSION   DATMKH010000420 DATMKH010000000
VERSION     DATMKH010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4028904"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..177)
                     /locus_tag="VNV63_05055"
     CDS             complement(<1..177)
                     /locus_tag="VNV63_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HXC62021.1"
                     /translation="MELVLATRNRHKVSEIRAILQGLSLTIRTLDDFPTVPEVVEDGA
                     TCRENAIKKARVIAR"
     gene            complement(182..904)
                     /gene="rph"
                     /locus_tag="VNV63_05060"
     CDS             complement(182..904)
                     /gene="rph"
                     /locus_tag="VNV63_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003334898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HXC62022.1"
                     /translation="MRLNGRKRSELRSVKITRDYIKHAEGSALIEMGDTKVICTASLE
                     ERVPPFLRNQGKGWITAEYAMIPRSTKERVQREATKGRLSGRTQEIQRLIGRSLRAVA
                     DLEALGERTVWIDCDVIQADGGTRTASITGAFIALVDALAFAVKQGLIEKIPLRDYLA
                     AVSVGKVNGELLIDLTYEEDSTADVDMNLVMTGDGRFVEIQGTAEKAPFNEKEMKALL
                     TLAKKGIRQLIKTQQGLLRGLL"
     gene            complement(996..2195)
                     /locus_tag="VNV63_05065"
     CDS             complement(996..2195)
                     /locus_tag="VNV63_05065"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXC62023.1"
                     /translation="MIEGLDYKKYPALYVDDEEMALETFKAQFRRDFTIHTALSGEEA
                     LRILSQQPIALIITDQRMPQMGGVALLEEVTKQYPNTIRMLMTAYSDMDIVIAAINKG
                     QVYRYFPKPYNEDELRESIKEGLERYYLIRERERLYAEKIELIKRMERTNRLTAVGTL
                     AAGMAHEINNPLVAINTFLQMVPQKYAEEEMDKEFWEHFHKVVISEVDRIRNLIGRLL
                     KYSRFSEKEELHLEETDINQILQDMATFLDNEAKRKGLTIQQDLTSGMPTALLDRERM
                     KQVFLNILLNAIQATFRGYIRIKSACIEEDGEKFLQIAISDTGIGISEEELQKLFNPF
                     FTTKQRGGSGLGLITCHQIVDEHRGTIDVQTEVGKGSTFIVTLPLDLRRYNRRRIERR
                     REEDRLI"
     gene            complement(2264..2610)
                     /gene="ssrA"
                     /locus_tag="VNV63_05070"
     tmRNA           complement(2264..2610)
                     /gene="ssrA"
                     /locus_tag="VNV63_05070"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT          547 a          749 c          639 g          712 t
ORIGIN      
        1 ccgagctatc acccgagcct tcttgatcgc gttctctcgg cacgtggccc cgtcttccac
       61 cacctcggga accgtcggaa aatcatccag cgtgcggatc gtaagactca aaccctgaag
      121 gatggcccgg atctcggaaa ccttgtgcct gttccgtgtg gctaggacga gctccatcct
      181 tctaaagaag acctctgagc agtccctgtt gggttttaat cagctgacga atgccttttt
      241 tcgccagcgt caagagcgct ttcatctctt tttcattaaa tggcgccttc tcagcggtcc
      301 cttggatctc gacaaatctg ccatctcctg tcattaccaa gttcatgtcc acatccgcgg
      361 tggaatcttc ttcatacgtg aggtctatca acaactcacc attcaccttc ccaacgctca
      421 cggctgccag gtaatctctg agcggaatct tttcgatcaa accttgcttc acagcaaaag
      481 ccaaggcgtc taccagggca ataaaggccc ctgtgatcga ggcggttcga gttccgccgt
      541 ccgcctggat cacgtcgcag tctatccaaa cggttcgttc tcccagggcc tcaaggtccg
      601 ccacggcacg caaagaccgc ccgatgagcc gctgaatctc ctgtgtgcga ccgctgagcc
      661 ggcctttagt ggcttctcgc tggactcgct ccttcgtcga gcgtggaatc atcgcgtatt
      721 cagcggtgat ccaccccttg ccctgattcc gtaagaaggg cggcacccgc tcttccagac
      781 tggcagtaca gatcactttg gtatcgccca tctcgatgag cgccgaaccc tcggcatgct
      841 tgatgtagtc tctcgtgatc ttgaccgacc gcagctcact tcgcttacgt ccattgagtc
      901 tcatggtttg gctacctttc ttgacgatcc cagggcaaaa atgaagggct attatagcct
      961 atcatctgtg aaatagcaat gcggggcagg gtggtttaga tcaggcgatc ttcttctctt
     1021 cttcgctcaa tccggcgacg gttatagcgg cggaggtcca gcggcagggt aacgatgaaa
     1081 gtactgcctt tccccacctc cgtttggaca tcgatcgtcc cgcgatgctc gtcgacaatt
     1141 tggtggcagg taatcaggcc tagaccgctt ccccctcgtt gcttggtggt aaaaaaggga
     1201 ttgaagagtt tctgtagttc ctcctcacta attccgatgc cggtatcgct gattgcaatc
     1261 tggaggaatt tctctccgtc ttcttcaatg caagcgcttt taatccggat gtagcctcga
     1321 aaggttgcct gaatcgcatt gagaagaatg ttaagaaaga cctgcttcat tcgctctcga
     1381 tccaatagcg cagtaggcat cccggacgtc aggtcttgtt gtatagtgag accttttcgc
     1441 ttggcctcgt tgtccagaaa ggtggccatg tcctgaagga tttggttgat gtcggtttct
     1501 tcgagatgga gctcctcctt ctcggagaac cgagaatatt tgagcaagcg gccaatcaga
     1561 ttgcggatcc gatccacctc gcttatgacc actttgtgga agtgctccca gaactccttg
     1621 tccatctcct cttctgcata tttctgggga accatctgaa gaaaggtatt gatcgcaacc
     1681 aagggattgt tgatctcgtg cgccatgccg gccgcaagcg tgcccaccgc cgtgaggcgg
     1741 ttggtccttt ccatgcgctt gatcagctcg atcttttccg catacaagcg ttcgcgttct
     1801 cggatcaggt agtagcgctc taatccctcc ttaatggact ccctcaactc atcctcattg
     1861 tatggttttg ggaagtaccg gtacacttgg cccttattga tcgctgcaat cacaatatcc
     1921 atgtcgctat aagcggtcat cagcatccgg atggtgtttg gatactgctt cgtcacctcc
     1981 tcgagcaagg cgacgccgcc catttggggc atccgttgat ccgtaatgat tagtgcaatg
     2041 ggttgttgcg aaagtatgcg gagcgcctct tcaccgctga gcgcagtgtg tatggtaaaa
     2101 tcccgtctga attgtgcttt gaaggtttcc aaagccatct cttcatcatc tacgtaaagg
     2161 gccggatatt ttttgtagtc taatccctcg atcatcacgg ttccgttaat cgtttggttt
     2221 tctatctctt ctcgatatcg agaaggccac gttcaaataa aattggtgga ggcggcggga
     2281 attgaacccg cgtccgaaga tcctctgcac aaggccccta catgcttagt ctatgttttg
     2341 atctcatccc tacccccgcc cttagaccgg ctggagaggg gaccagctta gatatgtctc
     2401 gccaaccctt tctaagcaaa aaggctagcc agcctgctat ctggtcgcct ccaagagaac
     2461 acaggcaatt ccccgagaga cgcgctgcgt ttaggcagcg agggccaact gagagttggc
     2521 agttgatttt ttttccattt gttttacgag ccaatggaac ctcggcatgc agccctgggc
     2581 ttcttatccc cgtcgaaacc gatcgccccc ctttcatatc aatcttgcta tgatcttctt
     2641 cccttga
//
LOCUS       DATMKH010000421         3440 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2110127, whole genome shotgun sequence.
ACCESSION   DATMKH010000421 DATMKH010000000
VERSION     DATMKH010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3440
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2110127"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1173
                     /gene="hisD"
                     /locus_tag="VNV63_05075"
     CDS             <1..1173
                     /gene="hisD"
                     /locus_tag="VNV63_05075"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HXC62024.1"
                     /translation="KAVRQQGDRAVLRFTKRFDRLPLKASELRVPQEWIKRAYQELKP
                     EQIESLEYAADRIAAFHIRQRQTTWTYDEDGITLGQQVRPLATVGLYVPGGKAAYPSS
                     VLMNAIPAKIAGVERIVISSPTPAGELNPALLVAADLAGVHEIYRVGGAQAIGAMAFG
                     TETIPRVDKIVGPGNQYVALAKRLVYGVVDIDLIAGPSEIVVIADDSADPRFVAADLL
                     SQAEHDEKAMAMLLTPSAKLARRVQTELAVQLRKLPRRKIATASLESYGAIVLVRDLQ
                     EAVELANQIAPEHLELEVEDPRALLARVKNAGAVFLGHYSPQALGDYIAGPNHVLPTG
                     GTARFSSPLSLDDFVKKTSLIEYSKEGLRRAKEQVIRIASMEGLDGHWKAVEIRMR"
     gene            1176..1769
                     /gene="hisB"
                     /locus_tag="VNV63_05080"
     CDS             1176..1769
                     /gene="hisB"
                     /locus_tag="VNV63_05080"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HXC62025.1"
                     /translation="MNRTATVERLTSETQVRIRLNLDGTGQGRIKTPLPFLDHMLTLM
                     TRHGFLDLSLSANGDVEVDDHHTVEDIGLVLGDAMQKALGKKQGIRRYGFSSIPMDET
                     LASVTLDLSGRPYLVYHVKVPGRTKIKEFDISLVEDFFQAFVNRSGTTLHINVAYGKN
                     PHHILESVFKAFGKALSQAVAKDERVQGALSTKGRLG"
     gene            1769..2392
                     /gene="hisH"
                     /locus_tag="VNV63_05085"
     CDS             1769..2392
                     /gene="hisH"
                     /locus_tag="VNV63_05085"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HXC62026.1"
                     /translation="MIAIIDYDMGNLRSVQKAFEKIGHAAIVTRDPKTIQDASHVVLP
                     GVGAFPDAMKNLERFKLIDPIVKGIEEGKPFLGLCLGLQLLFTEGEEFGIHRGLDILK
                     GRVVRFQISNNGTKTKLSIPHIGWNTIQMKTQSPLFKDVPNGSYFYFVHSYYVVQEDN
                     RWASTTTEYGLEFTSSIWKDNIFACQFHPEKSQDQGLKLLRRFAEMG"
     gene            2413..3066
                     /locus_tag="VNV63_05090"
     CDS             2413..3066
                     /locus_tag="VNV63_05090"
                     /inference="COORDINATES: protein motif:HMM:NF015724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HXC62027.1"
                     /translation="MPFASYLWLVLLLVSLPLLEGCGVKYVSGVTAPDFQEYPIKKIA
                     VLPFQNQQVLPDVKISNIPPVAEGSDHLLTQIFVERLQATSGYQIIPQEQVEAGFKTA
                     QAGGSGTITTRLTQEVGKALGADAVITGRVQRFTERAGGSLESQHPASVGFDVEMINV
                     GDGKTLWSSQYYETQKTLTEDVNTLPLFWKRGGKWLTARELAEYGIEEMIKTLPKKR"
     gene            3069..>3440
                     /locus_tag="VNV63_05095"
     CDS             3069..>3440
                     /locus_tag="VNV63_05095"
                     /inference="COORDINATES: protein motif:HMM:NF013168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HXC62028.1"
                     /translation="MQVIPAIDIKGGRCVRLRQGKMSEETVYAVNPVEVAKRWEQAGA
                     RLLHVVDLDGAVGGAPKNKKVIQEIATAVSIPLQVGGGIRDLKTIEDYRASGIHRVIL
                     GTAVVQQRTLLVDACQRFPKAI"
BASE COUNT          852 a          841 c         1003 g          744 t
ORIGIN      
        1 aaagcggtta gacagcaagg cgaccgagcg gtcctccgat tcacgaaacg ttttgacagg
       61 ctccccctga aggcctccga actcagggtc cctcaagaat ggatcaaacg ggcctatcag
      121 gaactcaaac cggaacagat agagagcctg gaatatgcag cggatcgaat tgcggccttt
      181 catatcaggc agaggcagac aacctggaca tatgatgaag acggaatcac tttgggccag
      241 caggtcaggc ccttggcgac ggtgggtctt tatgttccag gagggaaggc ggcctatcct
      301 tcatcggtct tgatgaacgc tatccccgca aaaattgcag gagtggagcg gatcgtgatc
      361 tcatcgccca cgccggcggg agagctcaac cccgccctgc tcgttgcggc cgatctggcc
      421 ggggtgcatg agatttatcg tgtggggggc gctcaggcga tcggcgcgat ggcctttgga
      481 accgaaacaa tcccccgggt ggacaagatc gtgggaccgg gcaaccagta tgtggccctg
      541 gcgaagcgct tggtttacgg ggtggtagat atagacctga ttgcgggtcc aagtgaaatc
      601 gtggtgatcg cggatgattc tgccgatccc cgttttgtcg cagcagacct cctctctcag
      661 gccgagcacg atgagaaggc gatggcgatg ctcttgacac cttccgctaa attggctcga
      721 cgggtacaaa cagagcttgc ggttcagctg cgaaagctcc cccgtcgaaa aatcgcaaca
      781 gcgtctttag aaagttacgg cgccattgta cttgtgcgag atcttcaaga agcggtggaa
      841 ctggcaaacc agatcgcccc ggagcatctg gagcttgaag tggaggatcc ccgtgcctta
      901 ctggctcgtg tcaagaatgc gggggcggtg ttcctcggcc actattctcc gcaggcgttg
      961 ggagactata tcgccggacc gaaccatgta ctacccacgg gaggaacagc gaggttttcc
     1021 tccccgcttt cattagatga tttcgtcaag aagaccagcc tgatcgagta ttcgaaagag
     1081 ggtttgcgtc gggccaagga acaggtcatc cggatcgcct ccatggaggg gctggacgga
     1141 cattggaaag cggtggagat acggatgagg taaaaatgaa ccggaccgca acagttgaac
     1201 gcttaacgtc cgagacccag gtcaggatca gactaaatct ggacgggaca ggccagggaa
     1261 ggatcaagac acctttgccg tttctggacc atatgctgac actgatgaca cggcacggct
     1321 tcttggacct ttctctttcg gccaacggag acgtagaggt ggacgatcat cacacggtgg
     1381 aggacatcgg cctggttttg ggcgatgcga tgcagaaagc gctggggaaa aaacaaggta
     1441 ttcgaagata cggtttttcc tccatcccga tggatgagac gctggcctcg gtgactctcg
     1501 atctttccgg ccggccctat cttgtctatc atgtgaaagt cccgggaagg acgaagatca
     1561 aggagttcga tatttcactg gtcgaggatt ttttccaggc ctttgtgaat cgaagcggaa
     1621 cgacgcttca tatcaatgtt gcttacggga agaatccgca ccatatcctg gagtcggtct
     1681 ttaaggcctt tggcaaggcc ttgagccaag cagttgcaaa agatgaacgg gtccaaggcg
     1741 ctctctcaac aaagggaaga ctgggctgat gatcgcgatc attgattatg acatgggaaa
     1801 tctccggagc gtccaaaagg cgttcgagaa gatcgggcat gccgccatcg tcactcggga
     1861 tcccaaaacc attcaagatg cgagccacgt ggtgcttccc ggcgtcggag cgtttccaga
     1921 tgcgatgaaa aatctggaac ggtttaaact gatcgatcct attgtcaaag gcatcgagga
     1981 aggcaaacct tttttaggac tgtgcctggg gctgcagtta ttgttcaccg agggggaaga
     2041 gttcggaatt caccgaggcc tggatatttt aaaaggccgg gtggttcgtt ttcaaatatc
     2101 caataacggg accaaaacaa aactttcgat cccgcacatt ggctggaata caattcagat
     2161 gaagacccag tcacccctct tcaaggatgt tccgaacgga tcatattttt attttgtcca
     2221 ttcctattac gtggtacagg aggataatcg ttgggccagc acaacaaccg aatacggatt
     2281 ggaattcact tccagcatat ggaaggacaa catctttgcc tgtcagttcc atccagagaa
     2341 gagccaggac caggggctca aactgctcag gaggtttgcc gaaatggggt aaccacgcac
     2401 cgacaacaat ttttgccttt cgcctcctat ttatggctgg tcttactgct ggtttcactg
     2461 ccgcttttgg aaggctgcgg ggtgaagtat gtcagcggcg tgacagcacc cgattttcag
     2521 gaatacccaa ttaagaagat tgcggtcctg ccatttcaga accaacaggt tttaccagac
     2581 gttaagatct cgaacattcc tccggtagcg gaaggatcgg atcatcttct gacccagatt
     2641 tttgtagaac gacttcaggc aacaagcggc tatcaaatca ttcctcaaga gcaggtcgag
     2701 gcgggtttta aaacagccca ggcgggcgga tcaggaacca tcacaacccg tctgactcag
     2761 gaggtgggta aggccctggg agcggatgcg gtaatcacgg gccgtgtgca gaggtttacc
     2821 gaacgtgctg gaggttctct ggaaagccaa catccggcat cggtgggttt tgacgtggag
     2881 atgatcaatg tcggagacgg caagactctc tggtcgagcc agtactatga aacacagaaa
     2941 accttgaccg aggatgttaa cacactcccg ctcttctgga aacggggcgg taaatggcta
     3001 acagctcgag agctggccga atacggcata gaagagatga tcaaaaccct gccgaagaag
     3061 agatagcgat gcaggtcatt ccagcgattg atatcaaagg gggccgttgt gtccgtctcc
     3121 gacagggaaa gatgagcgag gagacggtct acgccgtgaa tccggtcgag gtggcgaagc
     3181 ggtgggagca ggcgggcgca agattgcttc atgtcgtgga cctggacggg gcggtgggcg
     3241 gtgcacccaa gaataaaaaa gtgattcagg agatcgccac cgctgtttcc atcccgttac
     3301 aggttggtgg gggaattcgc gatttaaaga ccattgagga ttaccgagca tccggtatcc
     3361 atcgagtaat actcgggaca gcggttgtgc aacagcgtac tttgcttgtg gatgcctgcc
     3421 agagattccc caaggccatc
//
LOCUS       DATMKH010000422         1490 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1792472, whole genome shotgun sequence.
ACCESSION   DATMKH010000422 DATMKH010000000
VERSION     DATMKH010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1490
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1792472"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..974)
                     /locus_tag="VNV63_05100"
                     /pseudo
     CDS             complement(1..974)
                     /locus_tag="VNV63_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010230269.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
     gene            1163..>1490
                     /locus_tag="VNV63_05105"
     CDS             1163..>1490
                     /locus_tag="VNV63_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62029.1"
                     /translation="MCRVCILRLHQGLRQVVWGDSRQLRVDKEESQEVLVYILLVRLL
                     KLQVLAGIRRCRDSRLFEERRQEQELRLVVVGTLDEVHRKMYEVPRQDDIRKVYKLVG
                     RRMVYAQ"
BASE COUNT          296 a          339 c          439 g          416 t
ORIGIN      
        1 gttgtcacag ctccgaggcc tttatgcatc tcagattcga tgacggttcc tctagcacgg
       61 gcattcggat tggctttgag ttcaagaatt tcggcttgca aggctagcat ctcaagaagt
      121 tctttgatgc cttctccggt gacagcagag cagttgatcg taatagtctg tccgccccag
      181 gcttctggaa gaagattgat ttcagagagt tggcggaaga cgttttcaga gttgaagttt
      241 ggtttgtcgc atttgttaat cgcaacgacg atcggaacat tggctgtacg ggcttgttcg
      301 atcgcttcca tggtttgagc acggaggcct tcatctccag caacgactag aacgacgata
      361 tcagtgacat cggcgcctct agcacgcatt gcagaaaatg cttcgtggcc aggggtatct
      421 aaaatagtaa tatctccgac agcggtatgg gctttaaacg ctccgatatg ctgagtgatc
      481 gctccggctt caccgcttgc gacgttggcc ttgcggatgg tgtcgatcag gctggttttg
      541 ccgtggtcaa cgtggcccat gaaggtcaca actgggctgc ggagaacgag ctcgtcagga
      601 ggggtttctg agatttcttg tttaattgtc ttatcggtga ttttgagtcg ctcttcttcg
      661 ctggtatcga tggcgatttc gcaatcaaaa tcgtgaccga gaagttggat ggttgtttca
      721 tcatcgatga agtcattgag agtaatgaca acgcctttca taaagaggtt agagaggagc
      781 tgtgaggctt tgagtttcat ctcgaccgcg agctctttga tggtaattgg cacgcgaact
      841 tttaaagatt tagggcggac aatgagttct tcggctgaag atgacattct tctatggccg
      901 cggcctttgc gtggacgcca gccttcatcg ttttcatctc ggagcccttg tttatcgcgg
      961 gagtcaaaag cgcgatagac ttctggtttt ttatgagcct taatatcttt gacgtctttg
     1021 aattttcttg ctgcagcttc ttcgcgggat tttgcaggtg cgtgttcacg agaggatggt
     1081 tttgcaccag cacgagtatc cattggaggg ggtggacgtc ttccgaatcc acctgcaccg
     1141 ccagctcctg gaggtcttcc tgttgtgtag ggtctgtatc ctccggctcc accaggggct
     1201 ccgccaggtg gtctggggcg atagccgcca gctccgggtt gataaggagg agagccagga
     1261 ggtcttggtt tatatccttc tggtacgcct gctgaagctg caggttttgg ccggtatccg
     1321 ccgctgccgt gatagccgcc tgtttgagga gcggcgccag gagcaggagc tccggcttgt
     1381 cgtggttggt acgctggacg aggtccatcg gaaaatgtac gaggtgcccc gccaggacga
     1441 tatccggaag gtttataagc tggtcggtcg ccggatggtt tatgcacagg
//
LOCUS       DATMKH010000423         1017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_197493,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000423 DATMKH010000000
VERSION     DATMKH010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197493"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="VNV63_05110"
     CDS             complement(<1..392)
                     /locus_tag="VNV63_05110"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HXC62030.1"
                     /translation="MKKSSSFTIIGLLALTLGFASQAQAQMKVGTIDMQKIFTAYYKT
                     HDAEEKLKEAQKAYKDELDQRMDEYKKNLDVINKLNEEMNKPELSGASKDQKAQERDS
                     KISETKGLEKEISDFRQTREKQIQDQLKR"
     gene            complement(463..>1017)
                     /locus_tag="VNV63_05115"
     CDS             complement(463..>1017)
                     /locus_tag="VNV63_05115"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HXC62031.1"
                     /translation="GPLGGSVQVYGFDLDAAQYFHLPYDTILLLNGEVGTVENWGGSS
                     STGGIVPIFDRLYLGGASNLRGFKFRDVGPKDNEGNAIGGNTLTRWTIEYTIPIIERV
                     RFAVFYDGGFVNPGSWSFSPQKVPVPGNTQGKFSGGLNQDIGIGVRLDLPIGPLRLDY
                     GFPIEEDSFSSKSGQFQFSVGYQF"
BASE COUNT          232 a          277 c          244 g          264 t
ORIGIN      
        1 cgtttcaact gatcctgaat ttgcttctca cgcgtctggc gaaagtcgga gatttccttt
       61 tccagaccct tcgtctcaga gatcttggaa tcgcgctctt gcgctttctg atccttggaa
      121 gcgccgctga gttcgggctt gttcatctct tcattcaact tattgatgac gtcgagattt
      181 ttcttgtact catccatccg ttgatcaagt tcatctttgt acgctttctg cgcttcctta
      241 agcttttcct ccgcgtcatg agtcttgtag taggcggtga agattttttg catgtcgatc
      301 gtaccaacct tcatttgggc ttgcgcttga gacgcaaagc ccaaagtcaa ggccagcaaa
      361 ccgattatcg tgaacgatga ggatttcttc ataaaataga cctactgatt tactaatctg
      421 gggaaaccga ttggaggtga gaccggacga tacagctttt gttcaaaatt ggtagccaac
      481 actaaactgg aattgcccgc tcttgctgct gaagctgtct tcttcaatcg ggaagccgta
      541 atcaagccgc aatggcccga ttggcaagtc gagacgcaca ccgataccga tgtcctgatt
      601 tagccctccg gagaatttcc cttgggtgtt acccggaacc gggaccttct gcggactgaa
      661 actccacgag ccgggattga cgaaaccacc atcatagaaa accgcgaaac gcacccgttc
      721 gatgatgggg atcgtgtact caatcgtcca ccgggtcaga gtgtttcctc cgattgcatt
      781 tccctcgtta tccttcggtc cgacatcgcg aaatttgaac ccgcgaagat tgctggcgcc
      841 gcccagataa aggcgatcaa aaatcgggac gatcccaccg gtggacgagc ttccacccca
      901 attctcgacg gtcccaacct caccgttcag caggaggatg gtatcgtacg ggagatgaaa
      961 atactgggcc gcgtccaggt cgaatccgta gacctggaca ctgcccccga gtggacc
//
LOCUS       DATMKH010000424         1102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2763018, whole genome shotgun sequence.
ACCESSION   DATMKH010000424 DATMKH010000000
VERSION     DATMKH010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2763018"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(435..>1102)
                     /gene="glnA"
                     /locus_tag="VNV63_05120"
     CDS             complement(435..>1102)
                     /gene="glnA"
                     /locus_tag="VNV63_05120"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015935410.1"
                     /note="forms a homododecamer; forms glutamine from ammonia
                     and glutamate with the conversion of ATP to ADP and
                     phosphate; also functions in the assimilation of ammonia;
                     highly regulated protein controlled by the
                     addition/removal of adenylyl groups by adenylyltransferase
                     from specific tyrosine residues; addition of adenylyl
                     groups results in inactivation of the enzyme; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HXC62032.1"
                     /translation="GKTVTFMPKPIFGDNGSGMHTHQSIWKDGKPLFAGNEYAGISKM
                     CLYYIGGILKHAHALAAFTNPTTNSYKRLTPGFEAPVNLAYSNRNRSASIRIPMYSPS
                     PKAKRIEVRFPDPACNPYLAFAAMLMAGIDGIENKIDPGKPIEKDLYDLEPEEAAKIR
                     TMPGSLDQAISNLEADHAFLLKGGVFTPDVLETWISWKKTKEMDPMRLRPHPYEFFLY
                     YDA"
BASE COUNT          259 a          291 c          276 g          276 t
ORIGIN      
        1 tccggataca agaaagagca atggccaccc ccagcggaat agacccgcat tcgccagtct
       61 atgctccaaa atggcaagaa ttcccaacag cagaacaaaa acgccgttca gttgatgcat
      121 aaactcggat tcggccctct cctctggcga gccggggatt ggtgcggaag aatgtgcgtg
      181 cgacgcctct ccgtgttcca tctggcgtat ctcttgcgag tgtgaatggg cagggaggag
      241 aaaaaagatc agcgtcagca gtggggcaag tgagaggtgc aacatggcat caatttatct
      301 caactctctt gggcatgtca atagtctata gaaaaaggcc cgtgagtgca tcatctcacg
      361 ggcctttcag gtttgtctat cggattttaa cttcccacgc ctttctcaga agaggttgtc
      421 tcctctttcc ttttttatgc atcataataa aggaagaact catacggatg cggtctcaat
      481 cgcatcggat ccatctcttt tgtcttcttc cagctgatcc aagtctccaa cacatccggt
      541 gtgaacactc ctcctttgag gagaaatgca tgatccgctt ccaggtttga tatggcctga
      601 tccaggctcc caggcatcgt gcggattttg gcggcttctt ccggctccag atcatagaga
      661 tctttctcta ttggctttcc aggatcgatc ttgttctcaa tcccatcaat accggccatg
      721 agcatcgccg caaaggccag ataaggattg caggccggat ccgggaatcg cacctcgata
      781 cgcttcgcct tcgggctggg agaatacatc gggatccgga tagaggcgct tcggttccgg
      841 ttggaatagg ccaggttcac cggcgcctca aaacccggcg tcaaccgttt gtaagaattt
      901 gtggtcggat tcgtaaacgc ggccaaggcg tgggcatgct ttaaaatccc gccgatgtag
      961 taaagacaca tcttgctgat cccggcatac tcgttcccag caaacagagg ctttccgtct
     1021 ttccaaatag actgatgcgt atgcatgccg gatccgttat cgccaaatat gggtttcggc
     1081 atgaaggtca cggtcttccc at
//
LOCUS       DATMKH010000425         7019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_197752,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000425 DATMKH010000000
VERSION     DATMKH010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 7019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7019
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197752"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="VNV63_05125"
     CDS             <1..357
                     /locus_tag="VNV63_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA modification/repair radical SAM
                     protein"
                     /protein_id="HXC62033.1"
                     /translation="FKMNELILDSEGNLLLGHDPKEAWAMAHPSFFPLEASTAPYELL
                     IRVPGIGPISAKRIVEIRRSCSITDLRQLTRLGVVTKRAASYLLLNGRLPATVSPNKT
                     HIKPAQLDLWTPKMAR"
     gene            382..1197
                     /locus_tag="VNV63_05130"
     CDS             382..1197
                     /locus_tag="VNV63_05130"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXC62034.1"
                     /translation="MTNVSMVITDQSAVIGGRRLFYRTGGKGKPVVLIHGIGASSQIW
                     ARNLGSLARNYQVYALDLIGFGQSEKARPAYAVEDHVNCLLNFMNHLQIEKASLIGHS
                     MGGLIALDFRLTHPGMVEKIILVDSIALPNSKPSFTERLLCLPVLGELFMIFRSKLIL
                     RKFVSKEFYYRPELVTDEMRDPLMQADRAAVLKLTRTIPSTNRREAMKGIKAPTLILW
                     GDQDRAIPRAHALLLQQLIPKSQYRILKETGHHPQIEASEEFNQEVARFLAGG"
     gene            complement(1207..2340)
                     /locus_tag="VNV63_05135"
     CDS             complement(1207..2340)
                     /locus_tag="VNV63_05135"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HXC62035.1"
                     /translation="MTQKRLESELRDNFMAGFVIRTAIAFLLFLLAFLLWVSGQRNLP
                     LLMVGLLCTFEVLVNIPYYFLSRHAANLKFHFYRNITIDVLVITISIYFLGGLHAGFF
                     LLLYPVVIIYAGVVLSYRDCFVFAALSSLCMITLVALQATGIVPLTSLPDRLTPNSQK
                     VIMLLGYTIFFHAVAYLSGSLSDMIKSTRHSLIAAQHDLLRLEREKAEESARLAITDG
                     LTGLYNHRYFQEQLVKEVQRAQRYSSSLSLIMGDVDHFKNFNDNYGHLQGDNVLRHVA
                     AVLKAETRQTDVVARYGGDEFVVILPNAEKADALRMAENIRNRIRTELNSGQENKLLS
                     AATISFGVASFPMDALTPMGLIQKADESLYGAKNGGRDRVVSI"
     gene            2421..3680
                     /gene="dinB"
                     /locus_tag="VNV63_05140"
     CDS             2421..3680
                     /gene="dinB"
                     /locus_tag="VNV63_05140"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HXC62036.1"
                     /translation="MSNVSHSPYSMERTILHIDMDAFFASVEQVVNPNLRGRPIVVCG
                     SHTRTVVLTASYEARAFGVRTGMTVPEARRLCPDLICTPAHNDRYTDTCRQLLQIYER
                     YTPVVEVFSVDEAFLDLTGSVQLFESREAISRTIKAAVAKRFGLSCSIGIAPNKLLAK
                     LASGLEKPDGLVIILSKDIPRLLEDLPIRELCGIGPALEKELKAMNLETCGQLGGAPV
                     DLLTHRFGVTGFKLQEMGRGLDNAPVIPLNQTPEAKSIGHSMTLKRDLSDHREIESVL
                     FHLAEMVGQRARRGNYLGSKITVTFRYIDFETFNRQASLPKPSQDGIEIFQAAQKVLR
                     CTRLEQPIRLAGVSLSHLIKDPGQEALFEDLKRRASLLQTMDVINSRHGEFTLTWATL
                     RERLSKSGGHPGVISPAWRPQGTRNYR"
     gene            complement(3696..4676)
                     /locus_tag="VNV63_05145"
     CDS             complement(3696..4676)
                     /locus_tag="VNV63_05145"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HXC62037.1"
                     /translation="MRQEILKILGGHSASRVLMVANELGIFDLLSDRSMSAGDLANEI
                     GTNPKATERLCNALAAMSLLTKGRNRFRLPAKLKPLLTKNGKASLTHTISLSHEFWRF
                     WTDLGAFIRSGRPLTEMLTLIQKDPAMLEQFIHAMRDRGVFAARYINQVLDFQTVRRV
                     LDLGCGPGIYALEWARRYRNLKAVLYDIESVLPITQQYIQAYRLEDRVRVMAGDFVKD
                     PIGEGYDLVLLANALQMHGAQECQALIGKVYRAMVKNGRIIIHGFMMDPSGTHPKESA
                     LFSLTIGLVTPSGNAHSISDTVRWLRKAGFKKISRFSIDVVPPIVITAIR"
     gene            4813..5346
                     /locus_tag="VNV63_05150"
     CDS             4813..5346
                     /locus_tag="VNV63_05150"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HXC62038.1"
                     /translation="MIRYFLIIFALFLLFTPISLQAVVYKWLDEQGNVGFTDNPDSIP
                     EKYRQNATRPDGTTIPQKPKTNITPAVPPSTNPTEDAPVLDDQGRDKQWWRARVQEFR
                     HRKEVLLAEKEKLAANTEPLGNLGLGSIEANQQATKTKERLEQIDSEVKEIEYNLTVL
                     LPEEAREANAPPGWLRE"
     gene            5349..6080
                     /locus_tag="VNV63_05155"
     CDS             5349..6080
                     /locus_tag="VNV63_05155"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HXC62039.1"
                     /translation="MGILVSGGTDVGRIRRSNEDSYGIYTDLHLYVVADGMGGHVAGE
                     VASRLAVEAIRSFFAATLKNGRPLSPSQRLIQAIQQANENIIQTSKDDPRLIGMGTTV
                     VAALVDEDTAYVGHVGDSRVYLLRNQEIKQLTEDHSLLNEYLQKGLITPENSEGYPYK
                     HIITRALGSHPAVEVDLQTIDLQPGDCFLLCTDGLTNMLTEKDIRTVLTTMDNPEKGC
                     RRLIEVANSKGGEDNITAVLFQWNP"
     gene            6104..>7019
                     /locus_tag="VNV63_05160"
     CDS             6104..>7019
                     /locus_tag="VNV63_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXC62040.1"
                     /translation="MKKILVIDDEASVQESLRTVLKEDYEILSAMDGIQAIEVFHEQN
                     PHLLLLDISMPSLNGLEVLRKIRESDTSSPVIMLTASNMVKTAVQAMKMGATDYLTKP
                     FDIEELKLVIQKTIANRELESEVKLLRSEVQKRYSFNNIIGKSRVMQEIYNRIEQVAD
                     TKSTVLITGESGTGKELVARALHYRSVRRHKPFIAINCAAIPEGLIESELFGHERGAF
                     TDASSRRIGQFELAHEGTLFLDEISDLSPATQAKLLRVLQEREFTRLGATHPIRVDVR
                     LITASNKDLQAVIEKGLFREDLYYRINVV"
BASE COUNT         1804 a         1866 c         1815 g         1534 t
ORIGIN      
        1 tttaaaatga acgagctgat tttggattcc gaaggcaacc tgctcctggg acacgatccg
       61 aaggaggcct gggcgatggc gcatccaagc tttttccccc tcgaggcaag caccgcgccg
      121 tatgagctgc tcatccgcgt gccgggcatc ggaccgatat ccgcaaagcg gattgtggag
      181 atccgccgtt cttgctcgat caccgatctt cgacaactta cccgtcttgg cgtggtcacc
      241 aaacgggccg cctcgtatct tctcctcaac ggccgtctcc cggcaacggt ttcaccgaat
      301 aaaactcata tcaaaccggc tcagcttgat ctttggactc caaaaatggc aagataaacg
      361 gactaaaaag caggagaact gatgacaaat gtctcgatgg taattactga ccagtccgcg
      421 gttattgggg gtcgccgtct cttttaccgg accggcggca agggaaagcc ggtcgttctg
      481 atccacggca tcggggcctc ctcacagatc tgggcgcgaa acctcggttc cctggcccgg
      541 aattatcagg tctacgcttt agacctgatc ggttttggac aatccgagaa agccaggcct
      601 gcctatgctg tggaggatca tgtgaactgc cttctcaatt ttatgaacca tcttcagata
      661 gagaaggcat ctctgatcgg gcactcgatg ggcggcctga tcgctttgga tttccgcctt
      721 acacacccgg gaatggtgga aaagataatc ctggtagaca gcattgccct acccaattcg
      781 aaaccgtctt ttactgaacg cctgctctgc cttccggtgc tgggcgaatt gttcatgatc
      841 tttcgttcta aactgatttt gaggaagttt gtgtcaaagg aattttacta ccgccccgaa
      901 ctcgtgacgg atgagatgcg ggatcctctg atgcaggccg accgcgctgc ggtcctgaag
      961 ctgactcgaa ccattcctag tacgaaccgt cgagaggcga tgaaaggaat caaagccccg
     1021 accttgattc tctgggggga tcaggaccgg gcaattcccc gggcccatgc cctgctcttg
     1081 caacagctca tcccaaaatc ccagtaccgg atcttaaaag aaaccggaca ccatccccag
     1141 atcgaggcgt cagaagaatt taatcaagaa gtggcaaggt ttctagcggg aggctgaaat
     1201 tttaaattat atgctcacga cccggtcacg gccgccgttc ttggctccat agagcgactc
     1261 gtccgctttt tggataagcc ccatgggcgt gagggcatcc atcgggaagg aggcaacacc
     1321 aaaactgatc gtcgcggcgc ttagaagctt gttctcctgc ccggagttga gctcggttcg
     1381 gatccggttt cggatgttct cggccatgcg gagagcatcc gccttctcgg cattcggcag
     1441 gatcactacg aattcatcgc cgccgtatcg tgccaccacg tcggtctgac gggtctcggc
     1501 cttgaggacc gcagccacat gtcgcaagac attgtctccc tggaggtggc catagttatc
     1561 attaaaattc ttgaaatgat ccacatcccc catgattaag gaaaggctag aagaatagcg
     1621 ctgggcgcgc tgcacctcct tgacgagctg ctcctgaaaa taacgatgat tatagagacc
     1681 ggtcaatcca tccgtgatcg ccagtcgcgc actctcctcg gccttttccc gctccagcct
     1741 cagcaggtcg tgctgagccg cgatgagcga atggcgtgtg ctcttgatca tgtcagaaag
     1801 actgccggag agataggcaa cggcatggaa gaaaatcgtg taccctagaa gcatgatcac
     1861 cttctggctg tttggggtga ggcgatctgg tagagacgtg agcggcacga tcccggtagc
     1921 ctggagggca accagcgtga tcatgcagag gctgctcaag gccgcgaaca caaagcagtc
     1981 ccggtaggac aagaccacac cggcatagat gataacgaca gggtagagaa gcagaaaaaa
     2041 acctgcatgg agaccaccca aaaaatagat ggagatggtg atgaccagga catcaatcgt
     2101 tatgttccgg tagaaatgaa acttcagatt ggcagcatgg cgggagagaa agtagtacgg
     2161 gatattgacg aggacttcaa aggtgcagag caggcccacc atgaggagag ggagattgcg
     2221 ctggccgctg acccaaagga gaaacgccag gagaaacagc aggaaagcaa tggcggttcg
     2281 gatcacgaac cccgccataa agttatcgcg taactcgctt tcgagccttt tctgagtcat
     2341 ttctttacca tctaagtagc tgttttaata ttgaaaacat tgaaattcac ttactttaag
     2401 tgtaagcgaa accctgtaag atgtcaaacg tctcacactc cccctactcg atggaacgga
     2461 cgattctcca tattgacatg gatgcctttt tcgcttcggt cgagcaggtg gtgaacccaa
     2521 acttaagggg aagaccgatc gtcgtctgcg gttcccacac tcgaaccgtg gtgctcaccg
     2581 cctcctatga agcccgcgcc tttggtgtcc gaacaggaat gaccgttccg gaggcaagac
     2641 gtctctgccc ggacctgatc tgcaccccgg cgcacaatga ccgatacact gatacttgtc
     2701 ggcaactcct ccagatctat gaacggtaca caccggtcgt ggaggtcttc tccgtggacg
     2761 aggctttttt agacctcacc ggctcggtcc aactctttga gagcagagaa gcgatttcaa
     2821 ggacgattaa ggccgcggtt gcgaagagat tcggcctgag ctgctcaatc ggaatcgctc
     2881 ctaacaagct ccttgctaaa ctggccagcg gcctagaaaa accggatggg cttgtaatca
     2941 tcctgtcgaa agatattccc cggctgcttg aggatttacc gatccgagag ctctgtggca
     3001 tcgggcctgc gcttgagaaa gagttgaaag caatgaacct cgaaacctgc ggccaactgg
     3061 gcggggcacc ggtagatctt ctcacccacc gctttggcgt gacaggtttt aagctccaag
     3121 agatgggccg gggcctagac aacgcgccgg tcatcccctt gaatcaaacg cctgaagcca
     3181 aatccatcgg ccacagcatg acactgaagc gggatctatc cgatcaccgg gagatcgaat
     3241 cggtcctgtt tcatctggcc gagatggtgg ggcaacgggc aagacgaggg aattacttag
     3301 gaagcaaaat cacagtgaca ttccggtata ttgatttcga gaccttcaac cggcaggcat
     3361 cgcttcccaa accgagccag gatggtattg agatctttca agcggctcag aaggtcctcc
     3421 gatgcactag gctggaacag cccattcgac ttgccggggt cagtctctct catctgatca
     3481 aagacccagg acaggaggct ttgtttgaag acctcaagcg ccgggcaagt ctccttcaaa
     3541 caatggatgt gatcaacagc cgccacggag aatttacact gacatgggcc acgcttcggg
     3601 aacgcctttc taaatcaggc gggcatcccg gcgtgatctc tcccgcgtgg agaccccagg
     3661 gaacgcgaaa ttacaggtag caggacaatt caccactacc ggatcgctgt gataacgatc
     3721 ggtgggacca catcaatcga gaatcgggaa atctttttaa agccggcctt gcgtagccaa
     3781 cggacggtgt ccgagataga atgagcattt ccggaggggg taaccaagcc gatggtcagt
     3841 gaaaacaagg ccgattcctt aggatgcgtc ccgcttggat ccatcatgaa accgtgtata
     3901 ataattcgtc cgtttttaac catggcgcga tagactttac caatcaaggc ctggcattct
     3961 tgggctccat gcatctgaag ggcattggct aggagaacca ggtcatagcc ttctccaatc
     4021 ggatccttta caaaatcccc tgccatgacc cgaacacgat cctccaaccg gtaggcttgg
     4081 atatactgct gcgtgatggg gagcacggac tcaatgtcat aaaggaccgc cttaagattc
     4141 cgataccggc gggcccattc caaggcatag atccccggcc cgcaacccag atccaacacc
     4201 cgccgaaccg tttgaaaatc gagaacctga ttaatgtatc gcgccgcgaa gaccccacgg
     4261 tcccgcatcg catgaataaa ttgctccagc atagccggat ctttttggat cagggttaac
     4321 atctccgtca gcggtcggcc gctcctgata aatgctccca gatccgtcca gaatcgccaa
     4381 aactcatggg aaagcgaaat cgtatgcgtc aatgaggcct tcccgttctt tgtgagcagc
     4441 ggtttgagtt tggccggcaa acgaaagcgg ttccgtccct tggtcaagag agacatcgcc
     4501 gccagggcgt tgcacagccg ctcggtggcc ttcggatttg ttcctatttc attcgctaaa
     4561 tcacccgccg acatggaacg atctgagaga agatcaaata ttccaagttc attggctacc
     4621 ataagaaccc tggatgcgct atggcctcct aaaattttca gtatctcctg ccgcatggct
     4681 tcctccaaaa gaatggcctt tactataaac caacccgttt aaagaacaca agccgatcgg
     4741 taaccccgtt tgaaccagac tgctctggac aaccgacata agatgggcta gaataagttc
     4801 tgtggtgaac cgatgatacg atacttcctg ataatatttg cattatttct gctgtttacc
     4861 cccatctcct tgcaggctgt agtgtataaa tggctggatg aacaaggaaa cgtcggcttt
     4921 accgacaatc cggatagcat cccggaaaaa tatcggcaga acgctactcg accggatgga
     4981 acaacgatcc ctcaaaaacc caaaacaaac atcacgccgg ctgtgcctcc ctcaacaaat
     5041 ccaactgagg atgcccctgt tttggatgat cagggccgcg ataagcagtg gtggcgagcc
     5101 cgggtacaag aattccgtca tagaaaggaa gtgcttctcg ccgaaaaaga aaaactagcg
     5161 gcaaatacgg aaccgctggg caacctgggt ctaggcagta tcgaagcgaa ccagcaagct
     5221 acaaagacaa aggagcggct cgaacaaatt gatagcgagg ttaaagagat cgagtacaat
     5281 ctaacagttc tccttccgga agaagcacgg gaggcgaacg ccccaccagg ttggttgcgt
     5341 gagtaacgat gggcattctt gtctcgggcg gtacagacgt tgggcgaata cgccgttcca
     5401 atgaagattc ttacgggatc tatacggatc tccatctcta tgtagtagca gatggcatgg
     5461 gcggtcatgt tgctggggag gtcgccagcc ggttggcggt tgaagcgatt cgatccttct
     5521 ttgctgccac attgaagaat ggtcgtcccc tctccccaag tcaacgcctc atacaagcaa
     5581 ttcagcaggc caatgagaat atcattcaaa ccagcaagga tgatcctcga ctaattggaa
     5641 tggggaccac cgtagtagcc gcattggtag acgaggatac cgcatatgtc ggccatgtcg
     5701 gtgacagccg tgtttacctg cttcggaacc aagagatcaa acaattgacc gaggaccatt
     5761 ctctactcaa tgaatacctg caaaaaggac tcatcacacc tgaaaattcg gagggctacc
     5821 cctataaaca catcatcacc cgtgcgctag gaagccaccc cgccgtggaa gtagatctcc
     5881 aaacaatcga tctccaaccg ggcgactgct tcctcctctg tacggacgga ttaacaaata
     5941 tgctgactga gaaagacatc cgcactgtct tgacgacgat ggataatccc gaaaaggggt
     6001 gccggcgcct gatcgaagtg gccaattcca agggcggtga agacaatatt acggctgttc
     6061 tttttcagtg gaatccctga tcggcaagca tcccaaagca tcaatgaaga aaattctagt
     6121 catagatgat gaggccagcg ttcaagagtc cttgaggact gtcctcaagg aagattatga
     6181 gatcctctcc gccatggatg ggattcaggc catcgaggtt tttcatgagc aaaacccgca
     6241 cctgttgctc ctggacattt ccatgccctc gctcaatggt ctagaggtgt tgcgcaaaat
     6301 ccgcgagtcg gatacatcgt cgccggtgat tatgctcaca gcaagcaaca tggtgaagac
     6361 cgccgtgcag gccatgaaaa tgggtgccac cgactactta acaaagccat ttgacattga
     6421 agagttgaag cttgtcatcc agaaaacgat cgcaaaccgt gagcttgaaa gcgaggttaa
     6481 attactccgt tccgaggttc agaaacgtta tagttttaat aacatcatcg gcaaaagccg
     6541 agtgatgcag gagatctaca accgaatcga gcaggtagct gacaccaaga gcacagtctt
     6601 gatcacgggg gaaagcggta ccggcaaaga acttgtggcc cgagcactcc attatcggag
     6661 tgtgcgacgc cataaaccgt ttatcgctat taactgtgct gcgatccctg aaggtctgat
     6721 cgagagcgaa ctctttgggc atgaacgggg cgccttcacg gatgcctcca gccgccgcat
     6781 cgggcaattt gaactggccc atgagggcac cctcttcctg gatgaaattt cggatttgag
     6841 ccctgccact caagcgaagc tgctgcgtgt tcttcaggag agagaattca cccgtttggg
     6901 ggctacccat ccgatccggg tggacgtgcg tctcattacc gcatcaaata aggatctaca
     6961 agccgtcatt gaaaaaggcc tctttcgtga agatctgtat taccgtatca atgtagtct
//
LOCUS       DATMKH010000426         1071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3081293, whole genome shotgun sequence.
ACCESSION   DATMKH010000426 DATMKH010000000
VERSION     DATMKH010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3081293"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..858
                     /gene="galU"
                     /locus_tag="VNV63_05165"
     CDS             1..858
                     /gene="galU"
                     /locus_tag="VNV63_05165"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HXC62041.1"
                     /translation="MIKAVIPAAGLGTRFLPATKASPKEMLPLVDKPLIQYVVEEAVA
                     AGIEQIILVTGRGKRTIEDHFDVSFELEANLKENGKREALKEIQRISKLAEFCYVRQS
                     EAKGLGHAILSARHLVGNEPFAVLLGDDIIDSEIPALSQLMKLYRETGSPVIGIQEVA
                     KSEVGQYGIIEPQRVRNGVWRVLDLIEKPQVAEAPSRLGVVGRYLLPPMIFGILEHTS
                     PGRNGEIQLTDALKKLARKQTMYGCTIQGKRYDAGNKLGFLIATVEFGLKNPELGKQF
                     RSYLKSLKV"
     gene            871..>1071
                     /locus_tag="VNV63_05170"
     CDS             871..>1071
                     /locus_tag="VNV63_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HXC62042.1"
                     /translation="MAEPQDKPTEHLDIPDELPLLPIRDLVVFPSMVLPLFVGREISV
                     KAVEAAANSQRMILLVAQKTMDL"
BASE COUNT          259 a          243 c          315 g          254 t
ORIGIN      
        1 atcataaaag cggttatccc agcagccggt ttggggaccc gattccttcc cgcaacgaaa
       61 gcttctccaa aagaaatgct tcctcttgtg gacaagccgt tgatccagta cgtggtagag
      121 gaggcggtcg cggcggggat cgaacagatc attctggtca cgggccgtgg caagcgaacg
      181 attgaggacc attttgacgt ttcgtttgag ctcgaagcaa atcttaagga gaatggcaag
      241 cgtgaggctt taaaggagat ccagcgaatt tcaaagctgg ccgagttctg ttatgtccgg
      301 cagagcgagg cgaagggttt aggccatgcg attctcagcg cacgtcacct ggtggggaat
      361 gagcccttcg cggttctcct gggagatgat attattgata gtgaaatccc cgccctttca
      421 caactcatga agttgtatcg ggagacggga agtccggtga tcggtattca ggaggtggcc
      481 aaatccgagg tcgggcaata tggaatcatc gaaccccagc gtgtcagaaa cggtgtttgg
      541 cgtgttctgg atctcatcga gaagcctcaa gtcgctgaag cgccttcacg gctgggggtg
      601 gtgggccgtt atctgttgcc gccgatgatt tttggaattc tggagcacac gtctccgggc
      661 agaaacgggg agattcaact aacggatgcc ttgaagaaat tggcgagaaa gcagacgatg
      721 tatggttgta ccattcaagg taaacgctat gacgcaggca ataaactggg gtttcttata
      781 gcaacggttg agtttggtct caaaaatccg gaactgggga aacagtttcg ttcttatctt
      841 aagtcattaa aagtctagca gggaggagtc atggcagaac cgcaggataa accaaccgaa
      901 catctcgata taccggacga gctgcctctt ctgccgatcc gggatcttgt ggtgtttccg
      961 tccatggtgc ttcctctgtt cgtcggtcgg gaaatttctg tgaaggcggt ggaagccgcg
     1021 gccaacagcc agcgtatgat tctcctggtc gcccaaaaaa ccatggatct g
//
LOCUS       DATMKH010000427         1030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2111352, whole genome shotgun sequence.
ACCESSION   DATMKH010000427 DATMKH010000000
VERSION     DATMKH010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2111352"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            464..>1030
                     /locus_tag="VNV63_05175"
     CDS             464..>1030
                     /locus_tag="VNV63_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62043.1"
                     /translation="MHPYFSTPIHPWQNFNPFDIGMGQRPFPWVDPTAYQPMFNQPIY
                     NQAMYGGAFPGFQTNPYYAGVLPRPVFQVPHVPFSPEITHGMSPVSFPGFGMSGYLSP
                     ENFISGYGKTPGNYLPPEFLEFLRAELLRCGKALRTVAPALEHDTGNVKEQAHLAATA
                     QFFYALGLLYTRGLIIPPEVPGTSSRAEE"
BASE COUNT          252 a          286 c          243 g          249 t
ORIGIN      
        1 taacaaagcc gactccggat cgcgtccaac caagtcatcg cagatacgtg cggtcgcgaa
       61 tgcgacaagg attttcacag gtcacccccg aaatacagat ttcacctgat tgtcaagaat
      121 cgtgacagat gtacgatcgc ctcagacacg gaatagccac atcattcagt atcgatggag
      181 taggaagggg acgcgtctta tatcgatgta gagtacgtct cggtgtggaa aacttaaacc
      241 gctagttccg gctaccggcg aacctccggt ctgccaatcg attctttgat atctgtgttt
      301 aaagaaattc ccgagttgag gaatttcttg acacctctgc cttaggacga tcgaagtctt
      361 gaaggcaaaa atcccagcca agataaaaac cttactgcga tgaggttttc gctcgcgctg
      421 taagctcacg gaacagctca aaattcaaaa aaaggagtag ctcatgcatc cgtatttctc
      481 gacgcccatt catccttggc aaaacttcaa tccatttgat atcggcatgg gccaaaggcc
      541 attcccatgg gtggacccca ccgcttatca acctatgttt aaccaaccga tctacaacca
      601 agccatgtat ggaggtgcgt ttccaggttt tcagacgaac ccgtactatg caggtgttct
      661 ccccagacct gtcttccagg tgcctcatgt gccgttctca cccgagatca cccacggaat
      721 gtctccggta agttttccgg gattcggcat gtctgggtac ctttcgccgg agaacttcat
      781 ctccggctat ggcaaaacac cgggaaatta tcttcctcct gagttcctgg agtttctgcg
      841 cgcagaattg ctccgctgcg gcaaagcgct gcggacagtt gcccctgccc ttgaacacga
      901 cactgggaac gtgaaagagc aagcgcatct tgctgccacg gcccagttct tctatgccct
      961 tgggcttctg tatactcgcg ggctcattat tcctcccgaa gtacctggga cctcgagccg
     1021 agctgaggag
//
LOCUS       DATMKH010000428         1608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1479360, whole genome shotgun sequence.
ACCESSION   DATMKH010000428 DATMKH010000000
VERSION     DATMKH010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1479360"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1176
                     /gene="argS"
                     /locus_tag="VNV63_05180"
     CDS             <1..1176
                     /gene="argS"
                     /locus_tag="VNV63_05180"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HXC62044.1"
                     /translation="YYINDVGTQIETLGRSVYLRYREVMGQPTTLPEEAYQGDYIQEI
                     ARELAAKHGGEFLNRPEAESLPHFTSYAYSLILRGIEQDLERFGVRFDRWFSERSLYE
                     DKEVDQAIGELKRRGFLYEHEGAEWVRTTDFGDDKDRVVVRANGQKTYFASDIAYQRN
                     KFRRGFNRAINVWGTDHHGYVPRVKAVVEMLGYSPSSLVVLLVQLVNLLRGGKVMAMS
                     KRSGEFVTLKEVMEEVGVDAARYIFLTRRSDTPLDFDLEVAKKQSDENPVYYVQYAHA
                     RLASVQRQAKEQGLEIPTVSEVRLERLVTPEERALMRQLAFYPDLVEESAIALEPHRL
                     TFYLHDLAALLHNYYYQHRIISEDLELSRARLVLMTMVKTVIKNALGILGVSAPERM"
     gene            1178..>1608
                     /locus_tag="VNV63_05185"
     CDS             1178..>1608
                     /locus_tag="VNV63_05185"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008518866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HXC62045.1"
                     /translation="MGMKFDILHQETTTQARLGVIQTSHGTITTPVFMPVGSAGSVKT
                     LTPLELEELGTEIILSNTYHLYLRPGHEVIADLGGLHHFMSWDRPILTDSGGYQVFSL
                     GELCKVTDEGATFQSHLDGSLHFFSPEMTIEIQEALGADII"
BASE COUNT          366 a          405 c          468 g          369 t
ORIGIN      
        1 tattatatca atgatgtcgg aacacagatc gagacactgg gccgttcagt atatctccga
       61 tatcgcgagg ttatgggtca gcccacgaca ctccctgaag aagcctatca aggggattat
      121 atccaagaga tcgcccggga attagcggcc aagcacggag gagagtttct gaacaggccg
      181 gaggccgaat cacttcctca tttcacctct tacgcataca gtttgattct ccgcggaatc
      241 gaacaggact tagagcggtt tggggttcgg tttgaccgct ggttctccga gcgcagcctc
      301 tacgaggaca aagaagtaga tcaggcgatc ggagaattga aacggcgggg ttttctctat
      361 gagcatgagg gggccgagtg ggtcagaacg accgactttg gggatgataa agatcgggtt
      421 gtggttcggg ccaacggcca gaagacctac ttcgcctcgg acattgccta tcaacggaat
      481 aaatttcgta gagggtttaa tcgggcaatt aatgtctggg gcacggacca ccacggctat
      541 gtgccgcgcg tcaaggcggt agttgagatg ctcggctatt ccccgtcgtc cctggtcgtt
      601 ctgcttgttc agttggtcaa ccttcttcgc ggggggaagg tgatggcgat gtccaagcgc
      661 tccggggagt ttgtgacgct taaggaagtg atggaagagg tcggtgtgga cgcggcacga
      721 tacatctttc tcacccggcg ttccgacacg ccgttggatt ttgatctgga ggtcgcgaaa
      781 aaacagtcgg atgagaaccc ggtctattat gtccagtacg cccatgcacg actggccagc
      841 gttcagcgac aggccaaaga acagggtctt gagattccaa ccgtcagtga ggttcgactc
      901 gagcggttgg tcacgcccga agagagggcg ctgatgcgcc aactcgcttt ctacccggat
      961 ctcgttgaag agagcgcgat cgccttggaa ccgcatcgct tgactttcta tctccatgat
     1021 ctggccgcgc tgcttcacaa ctattattat cagcaccgga tcatttcgga agacctggag
     1081 ttgagccggg ctcgattggt tctaatgacg atggtcaaaa ccgtgatcaa aaacgctttg
     1141 ggtatcctgg gtgtctcagc cccggaacgg atgtaaaatg ggcatgaaat tcgatatact
     1201 gcaccaggag accacaacac aggcacggct tggagtgatc cagacgtccc atgggacaat
     1261 tacaacgccc gtttttatgc cggtcggatc ggccgggagc gtgaagacct taacgccgct
     1321 tgaactggaa gagctgggaa ccgagatcat tttgtcgaat acctaccacc tctatctccg
     1381 gcccgggcat gaggtcattg ccgatctggg cggtcttcac cactttatgt cctgggatcg
     1441 ccccatcctc actgacagcg gagggtatca ggtcttcagt ctcggagagc tctgcaaagt
     1501 gacggatgag ggagcaacgt ttcagtcgca tctggacggt tcgctccact tcttctctcc
     1561 ggagatgaca attgagattc aggaggcgct cggggcggac atcattat
//
LOCUS       DATMKH010000429         1116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3703144, whole genome shotgun sequence.
ACCESSION   DATMKH010000429 DATMKH010000000
VERSION     DATMKH010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3703144"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..845
                     /locus_tag="VNV63_05190"
     CDS             <1..845
                     /locus_tag="VNV63_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62046.1"
                     /translation="YGQIEKDNLRNRRQHSFDFIRTRLVLLDFILNNQDLAYFETEQD
                     KVSYFCEQLEISKDLLPAKVYEGASGSRPTIRYFVDKFPLFLAPPLPGAPAVVTLSFV
                     DSGFETPSSFSGHVNAYQGLFRQLKTFRFLYIAPKDVHFRTAEDRFRALVKRPLESDV
                     SSEVLRYFQVRRKWDNREYVVPVTEDFEFLNDARRRFHGSRFERLYESWRTGELKELD
                     LRREFSQLTPDRTVFFDTYLVKGHWSPVSEHRNDGERRMKDTNHHPVHRSVHLDGERK
                     LVGA"
BASE COUNT          258 a          291 c          300 g          267 t
ORIGIN      
        1 tttacggcca gatcgagaaa gacaacctcc gcaaccgccg ccaacattcg tttgatttca
       61 tccgtacgcg cctcgtcctt ttggacttca tcctgaacaa tcaggacctc gcgtattttg
      121 aaaccgagca agacaaggtg agctatttct gcgaacaatt agagatttcc aaggacctcc
      181 ttcccgccaa ggtttacgaa ggcgcttcgg gcagccgtcc tacgattcgt tattttgtag
      241 ataaatttcc tctcttcctt gccccgcctc tcccaggggc tccggctgtg gtcacgctga
      301 gctttgtgga ttcggggttc gaaacgcctt ccagcttctc aggtcacgtc aacgcctatc
      361 aggggttgtt ccggcagctg aaaacttttc gctttcttta catcgcacca aaggatgtcc
      421 atttccgaac ggctgaagac cgcttccgtg ccttggtaaa gaggccgctt gaatcggacg
      481 tttcgagtga agtcctccgc tacttccagg ttcgcaggaa gtgggacaac cgcgagtatg
      541 tggtgccggt aactgaagat tttgaattcc tgaacgacgc gcggcggcgg tttcacggga
      601 gccggtttga aaggctgtac gaatcatggc gtaccggaga gctaaaagag ctcgatctgc
      661 gaagagaatt ctcacaactg actcccgata gaaccgtgtt cttcgatacg tatctggtta
      721 aaggccattg gtctccagtt tctgaacacc gtaatgacgg tgaacggcgc atgaaagaca
      781 caaatcacca tcccgttcac cgttccgttc acctggatgg tgaacggaag ttagtgggag
      841 cgtagaggtt gcgcatggcg cggcagtcaa ttgtggacgt ggacaaaaca ccgagttggg
      901 ctggagcgac cccacttttg ggagccttcg gctgcccaaa gaatcatggg gtcgctccag
      961 cccagggaag ggaatgcgcg ttccctttac aacgtgtttt gcccgcgtcc atggaagctg
     1021 ataactcaca aggaggaagg atatgtcgat catcaataaa cgaccggaac tcgttaagag
     1081 ggagtacgag ttggaggata tcgtcgtctc gaccgt
//
LOCUS       DATMKH010000430         1143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4675198, whole genome shotgun sequence.
ACCESSION   DATMKH010000430 DATMKH010000000
VERSION     DATMKH010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4675198"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VNV63_05195"
     CDS             complement(<1..272)
                     /locus_tag="VNV63_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXC62047.1"
                     /translation="MSKKILIIEDERDIRDLVAHYLTKDGYKVEAASDGAKGFSLAKT
                     MKPDLIILDLMLPELDGLEVCKKLRADPKTAVMPIIMVTAKGEETD"
     gene            complement(277..>1143)
                     /locus_tag="VNV63_05200"
     CDS             complement(277..>1143)
                     /locus_tag="VNV63_05200"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXC62048.1"
                     /translation="NLIAQSLTSYGLDWKKKGELPKLLKRLTAVNGIRWIRLFYAYPT
                     DLTDDLIELIAAEEKICSYVDLPLQHIDDEILRKMRRKGDSRLIRSLLEKLRSRIPHL
                     FMRTTFIVGFPGETEAQFGKLLDFVGEGWFNRIGVFGYSHEDGTPAHEFSDSVSVEEK
                     NERRNRLTTLQEEVSLSKNHALIGTIQEVMVEGRDEETQELFGRLEGQAPEIDGVVIL
                     EQRANASVGRGRLHPVLPVEGDGRSPIIKGQAPEIDGMVALEGDAGPGEFVKVEITGA
                     TELDLSGKILGA"
BASE COUNT          250 a          359 c          250 g          284 t
ORIGIN      
        1 ttgtccgtct cctcaccctt tgcagttacc atgatgatcg gcatgaccgc agttttggga
       61 tcggcccgca gcttcttgca cacctccaga ccgtccaatt ccggcagcat gagatccaga
      121 atgataagat cgggcttcat cgtcttcgca aggctaaaac ccttcgcacc atctgaagcg
      181 gcctcaactt tatatccgtc tttggtcaaa taatgtgcca ccaggtctcg gatatcccgc
      241 tcgtcttcga tgattaagat ttttttagac atggaatcat gctccaagga tcttcccgga
      301 aagatccagc tcggtcgctc cggtaatctc aaccttcaca aactctccgg gtcccgcatc
      361 tccttccaga gccaccatac cgtcaatttc aggcgcctgg ccttttatta taggagagcg
      421 accatccccc tccaccggca gaaccggatg gagcctcccc ctccccacgc tcgcattcgc
      481 tcgctgctct agaatcacca ctccatctat ttcgggcgcc tggccctcca ggcggccgaa
      541 gagctcttga gtctcttcat ctctgccttc taccatcact tcctgaatcg tcccgatcaa
      601 cgcgtggttt ttcgacagcg agacctcttc ttgaagcgtc gtcagccggt tgcggcgctc
      661 atttttttcc tccactgaga cggaatccga aaattcatgg gccggcgtcc cgtcctcatg
      721 ggaataacca aacaccccga tgcggttgaa ccagccctcg cctacaaaat ccagtagctt
      781 cccaaactgc gcctcggtct ctcccggaaa accgacgatg aaggtggtcc gcataaaaag
      841 gtgcgggatt cttgaccgta atttctctaa aagagaccgg atcaggcggc tgtcaccttt
      901 ccgccgcatc ttcctaagaa tctcatcatc gatatgctgc aagggaaggt ccacatagct
      961 gcagatcttc tcttctgcag cgatcaactc tatcagatca tccgtcaagt cggtcggata
     1021 ggcatagaag agccggatcc accgtatacc gttaacagcg gtcagacgct tgagcagttt
     1081 cggaagttcg cctttcttct tccaatctaa accatagctg gtcaggctct gggcgatcag
     1141 gtt
//
LOCUS       DATMKH010000431         1155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1479646, whole genome shotgun sequence.
ACCESSION   DATMKH010000431 DATMKH010000000
VERSION     DATMKH010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1479646"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            345..>1155
                     /locus_tag="VNV63_05205"
     CDS             345..>1155
                     /locus_tag="VNV63_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HXC62049.1"
                     /translation="MPTLATTVDGLNLPNPFIIASGPPGTNLNVIARAFQEGWGAVIA
                     KTVSLEAAKIINVAPRYAKLFSNEKQEVVGWENIELISDRPLKIWIEEFKRCKDRFPD
                     RILIASVMEEYRKDAWVEIIERCQETGVDGFELNVSCPHGLTERKMGSAMGEDPEILE
                     EVCGWVAAASKKPVWAKMTPNVARVEDPSRAALRGGAQGISLINTIRCVMGVNLDTLR
                     PEPTVEGYTTPGGYSSKAVKPIALRMVMEVATMVRNEFPGRTISGIGGIENG"
BASE COUNT          299 a          292 c          319 g          245 t
ORIGIN      
        1 atttagacac gagaaggtat tcggcatcgg cgaggaacct attttatccg cagatatttg
       61 caacacaatt taataatgta gttttctcta tgtaagaagg gtggagtagt actggagctg
      121 cgatcttttg cctagacgtg cagacagaac accctcagac cgtaccctac gggacggatc
      181 gatttgccat ggatccagcc ccgaaagctt tcggggctgg ctaccttcat tctgccccct
      241 acggggagag ggaatcccgt ttcccgccga atgcgctctt gaattttcta cggaaagatc
      301 tcaggttgtt cgcttacctt agcaacagag aggggaacag gaaaatgccc accctagcaa
      361 ctactgttga cggattaaat ctacccaatc cgttcatcat cgcgtccggc ccgccaggga
      421 ctaacctgaa tgtaatcgcc agagctttcc aggaaggttg gggagctgtc atcgctaaaa
      481 ctgtcagcct cgaagctgcg aaaatcatca atgttgctcc ccgctacgcc aaactgtttt
      541 ccaacgaaaa acaggaagtc gtcggctggg aaaacatcga gctcatcagc gatcgacctc
      601 ttaagatctg gatcgaagaa tttaagagat gcaaagaccg tttcccggac cggatcctga
      661 ttgcctccgt aatggaagaa taccgaaagg acgcctgggt cgagatcatt gagcgatgtc
      721 aggaaacggg tgtagacggt tttgaattga acgtctcctg cccgcacgga ttgacggagc
      781 gaaaaatggg atccgccatg ggagaagatc cggagattct ggaagaagtc tgtggctggg
      841 tagcagcagc aagcaaaaag ccggtctggg caaagatgac gcccaatgtt gcccgggtcg
      901 aggacccgag tcgagcggct ctccgaggcg gcgcgcaggg aatcagtctt atcaatacta
      961 tccgatgcgt aatgggagtg aatctcgaca cattgcggcc ggaacccacc gtcgaaggct
     1021 acacgacgcc cggcggttac tcttctaaag cggtcaagcc aatagcgctc cgcatggtga
     1081 tggaagttgc caccatggtg cggaacgaat ttcctggtcg gacgatctcc gggatcggcg
     1141 gaatcgaaaa cggcg
//
LOCUS       DATMKH010000432         1326 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3082358, whole genome shotgun sequence.
ACCESSION   DATMKH010000432 DATMKH010000000
VERSION     DATMKH010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3082358"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            19..>1326
                     /locus_tag="VNV63_05210"
     CDS             19..>1326
                     /locus_tag="VNV63_05210"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HXC62050.1"
                     /translation="MHNKAVENIYSDQVLSNEGEVTFFDDIGRESLGVYEQLGTAGLD
                     WGLVITISTDEAFAPAVHLTRLIAFGGFVILIVAILAALTFGHLLSRPIVQLVDTAER
                     IGSGDLSARAPISSKDEIGFLAERFNYMIDQVEERNRHVHKILETVNEGLFLMGPDFI
                     IQAGYSKITEAIFQRKIDGISFLDLLRPAPESGLQPIVTPEALLATQHYLELVLNPRI
                     KEKLIQQTNPLGEIEFKELRPNGAQRSKFLEFKFNRVVEGGKTTQIMVTVLDSTSRIA
                     LTRQISENETKTKSQIEMLFGIMHLDPPILAEFLNHAKSEVTVMLNLLEAEQFGSHAG
                     ESSTERSGRYLRLLQKISRSIHLIKGNAAMLRLSYFEDLANRLEEKIATVRGDGALSG
                     EQFLPITTGLASLLDQIGMTHDLITRLLSMQKVFGKEAGESGQT"
BASE COUNT          317 a          334 c          366 g          309 t
ORIGIN      
        1 acggtgcttg gcggcccatt gcataacaaa gcagtcgaga acatctattc cgaccaggtt
       61 ttatcaaacg aaggcgaggt aaccttcttt gatgatatcg ggcgggagtc gctcggggtg
      121 tacgagcaac ttggcacggc aggactggac tggggactcg tcatcacgat cagcaccgat
      181 gaagcgttcg cgccggccgt tcaccttacc cggttaattg catttggggg ctttgtcata
      241 ttaatcgtcg ctattctggc tgccctgaca ttcgggcatc ttctctcacg gccgattgtc
      301 cagttggtcg atactgcgga gaggattggc tccggcgatc tttctgcccg ggccccgatc
      361 tcaagcaagg atgaaatcgg gtttctggcg gagcggttca actacatgat cgatcaagtc
      421 gaagagcgga accgccacgt tcacaagatt cttgagacgg tcaatgaggg cctgtttctg
      481 atgggtccag atttcatcat ccaagccggc tattccaaaa tcaccgaagc aatttttcag
      541 cggaagatag atggtatttc ctttctggac cttctccgac cggctccgga atcagggctt
      601 cagccgatcg tcaccccgga agcgttgctg gcgacccagc attatctcga actggtgttg
      661 aatccccgca tcaaggaaaa gctcatccag cagaccaacc cattgggcga aatcgagttt
      721 aaagagcttc gaccgaacgg ggctcagcgc agcaagtttc tggaattcaa atttaaccgg
      781 gttgtcgaag ggggcaagac gacacaaatc atggtaacgg tgctcgattc tacctctcgg
      841 atcgcgctga ccaggcaaat ttcagagaat gagacgaaaa ccaaatctca gattgaaatg
      901 ttgtttggga tcatgcactt ggatccgccg attctggcgg agtttctgaa ccacgccaaa
      961 tcggaagtga ccgtgatgct caatcttctg gaggcagagc aatttgggag tcatgcgggg
     1021 gaatcgagca ctgagcgttc cggccgatac ttgcggctgc tgcaaaaaat ttcgcgctcg
     1081 attcacctga tcaaaggaaa cgctgcgatg cttcgtcttt cttattttga ggacttggca
     1141 aaccggctcg aagaaaaaat tgccacagtg cgaggagatg gcgcgcttag tggagaacag
     1201 ttcttgccga tcacgaccgg tttggcctcg ctcctggacc agattgggat gacccacgat
     1261 ttgatcaccc ggttgctatc gatgcaaaag gtctttggca aagaggccgg ggaaagtggc
     1321 cagacc
//
LOCUS       DATMKH010000433         1128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3082441, whole genome shotgun sequence.
ACCESSION   DATMKH010000433 DATMKH010000000
VERSION     DATMKH010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3082441"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..639
                     /locus_tag="VNV63_05215"
     CDS             91..639
                     /locus_tag="VNV63_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62051.1"
                     /translation="MNKTEVQDGKNSLDQLEDQGKKRQDSLYLSPKHRDRLIKRLATC
                     GLPVREIVLLSGASGRTVARTLQRLGISRKKYELPQYPSPRKRHAKSLETILTQYYGI
                     PFDELQLDSLHQLARRNGISLKKLFDLIRQHVSPSRWAIRTCLNCNQPSLTSSPADRY
                     CSLCRRKVKKAREGLEEGTIYE"
     gene            complement(665..>1128)
                     /locus_tag="VNV63_05220"
     CDS             complement(665..>1128)
                     /locus_tag="VNV63_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HXC62052.1"
                     /translation="HTGMRQGELLALTWDGVDLDRRTVTIIQSKNGDPLTNPINLTVQ
                     ALLKKRAKARPVETNLVFFSPVHAPIDDDYLRRVFTSAAAKADIEHFHFHGLRHTFAT
                     RLIQAGVDLYKVQRLLGHKSLMMTQRYAHHCPDSLRDSVDILDRKDGTITN"
BASE COUNT          287 a          322 c          280 g          239 t
ORIGIN      
        1 agcaagcgcc gaaaaccaaa aatgtccgga ataaaatgat ccgcaagacg gcccgtccac
       61 aaacagtcca ggatccggcg agaagtaacc atgaacaaga cagaggttca agacggtaaa
      121 aattctcttg atcagcttga ggatcaaggt aagaaacgac aagattcttt atacctctca
      181 cccaagcacc gggatcgtct gatcaagcgt ttggccacct gcggccttcc cgtccgggag
      241 attgttttgc tctccggcgc atccggccgg accgtggccc gtactctgca acgattggga
      301 atcagtcgaa agaagtatga gttgccacaa tatccatctc cacggaaacg ccacgctaag
      361 agcctggaga caattctgac tcaatactat ggcatcccat tcgatgagct ccaactggac
      421 tccctccatc aactggccag aagaaatggt atatccctga aaaagctttt cgatctcatc
      481 aggcaacacg tctccccctc ccgctgggca atccgaacct gcctcaactg caaccaaccg
      541 tctctcacct catcgcccgc ggatcgttac tgttccttgt gtagaaggaa agtaaagaag
      601 gcccgggagg gattggaaga gggcactatt tatgagtaaa tcatcttttt taaggaccag
      661 cctatcaatt tgtaatagtc ccatccttgc gatcgagaat gtccaccgaa tcccgcaggc
      721 tgtccggaca atgatgtgca taccgctgcg tcatcatcag cgacttgtga cccaggagcc
      781 gctgcacctt gtacagatcc acgccggcct ggatcaaccg cgttgcgaaa gtatggcgca
      841 ggccgtggaa gtggaaatgt tcaatatcgg ccttcgcggc cgccgaggtg aaaacccgtc
      901 gaagataatc gtcgtcaatc ggagcgtgga ccgggctgaa gaagaccagg ttggtctcga
      961 ctggcctggc cttcgcccgc ttcttcaata acgcctgcac ggttaggttg atggggttgg
     1021 tcaatggatc cccgttcttg gactgaatga tcgtcaccgt cctgcggtca agatccacac
     1081 catcccacgt cagcgccaga agttcgccct gccgcatacc ggtatgga
//
LOCUS       DATMKH010000434         1896 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1794414, whole genome shotgun sequence.
ACCESSION   DATMKH010000434 DATMKH010000000
VERSION     DATMKH010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1794414"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1098)
                     /locus_tag="VNV63_05225"
     CDS             complement(<1..1098)
                     /locus_tag="VNV63_05225"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXC62053.1"
                     /translation="MSSEFDVAIIGGGPAGSVAGSVLARHGRRVVIFEREKFPRFQVG
                     ESTIPASQDTLQRIGVKAKIDQAGFLIKHGGEIVSACGGARQKFFFRNALNPRWKTAY
                     QVDRMTFDDILLRHAQSLGCEVREETKVESVEFNADGVTLHCKNDPVPTKAAYVIDCS
                     GRSSLLANRFQLKEQFPHLKKFSVYSYYQRTSKSNDPEDAYTRMIRGSDAWFWVIPIS
                     DGKISVGVVMDLQKYQQLKLSPEAALARTIEEQPEVRKWVENTTRYMPVFATADFSYA
                     VKQLTGDRWIVAGDAAGFIDPVFSSGIYIAIYSGEKAGETINRVLTDPAKKDAAYRRY
                     EQAVQKRFRSYLKLSSAWYTKEFIEIFLHPKE"
     gene            1306..1785
                     /locus_tag="VNV63_05230"
     CDS             1306..1785
                     /locus_tag="VNV63_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002648566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXC62054.1"
                     /translation="MQVQKITPFLWFDNNAEEAMNFYVSVFKNSKVLSIHHSGKAGPG
                     EDGTVMVVYFELDGVRFLGLNGGPMNTFSEAISFWVYCDTQEEIDDLWEKLTAGGGEP
                     SKCGWLKDKFGLSWQIVPSILLELLDDKDPVKRDNVLQAVWEMEKLDMRKLQEVAAK"
BASE COUNT          439 a          455 c          530 g          472 t
ORIGIN      
        1 ttccttcgga tggagaaaga tctcgatgaa ctcttttgtg taccaggccg aagagagttt
       61 caggtaagag cggaaccgtt tttgcactgc ctgttcgtag cgacgatagg ctgcatcttt
      121 tttggccgga tcggttaata cgcggttgat ggtttcaccc gctttttcgc ccgagtaaat
      181 agcgatgtag atcccgctgc taaagacggg gtcgataaag ccggcggcgt ccccggccac
      241 aatccagcga tctccggtaa gctgcttcac cgcataagag aagtcggcgg tggcaaacac
      301 cggcatataa cgagtggtgt tctctaccca ttttcggact tccggttgtt cttcgatggt
      361 gcgggcgaga gcagcttccg gcgaaagctt gagctgctga tatttctgta aatccatcac
      421 cacacccacg ctgatcttgc cgtcagagat tgggatcacc caaaaccaag catcactacc
      481 tcgaatcatc cgcgtgtaag cgtcctccgg gtcattcgat ttgctggttc gttgatagta
      541 agagtagacg gaaaacttct taagatgggg aaactgctct tttagttgga agcgattggc
      601 cagcaggctg cttcggccgg agcagtcgat cacatatgcg gccttggtcg ggaccggatc
      661 atttttgcaa tggagcgtta ccccatccgc gttgaattcg actgattcaa ccttggtctc
      721 ttctctgacc tcgcatccta aactctgtgc gtgtcggagc aggatgtcgt caaaagtcat
      781 gcggtcgacc tgataggcgg ttttccaacg cggattaagc gcgttacgga aaaagaattt
      841 ttgccgggcg ccgccgcagg ccgaaacgat ctcgccgccg tgcttaatca aaaatccggc
      901 ctgatcgatc ttcgctttaa cgcctattct ttgcagcgtg tcctggctgg ccggtatggt
      961 ggattcaccg acctgaaatc ttgggaattt ttctcgctcg aagatcacga cgcgacgtcc
     1021 gtgccttgct agtactgatc cggctacgct gcctgccgga cccccgccga tgattgcgac
     1081 atcaaattcg gaagacattt tcaccgcaga atagaggagg agtggccggg agtcgagggg
     1141 ctaactatta actagtaacc agtgatcagt gatccgacgt cgccaaggct atgtcggaca
     1201 ggttggtggt tggccgtttt cgctcgtggg atatgcgtgg ctcctaaatt tgtcccataa
     1261 cccccgagct cattcgtcgt ttctgtgaaa agaaagacac caactatgca ggtacaaaag
     1321 attacaccgt ttttgtggtt cgataacaac gccgaggagg cgatgaattt ttatgtctcg
     1381 gttttcaaaa actccaaggt cttgagtatc catcactccg gcaaggcagg cccgggagaa
     1441 gatgggactg tgatggtcgt atactttgaa ttggatggcg tgagatttct gggactcaat
     1501 ggcggtccga tgaatacctt ctcagaggcg atctcgttct gggtttactg tgacactcag
     1561 gaagagatcg atgacctttg ggaaaagctc actgccggcg gcggcgagcc gtcaaaatgc
     1621 ggctggctaa aagacaaatt cgggctctcc tggcaaatcg ttccctcgat tctactagag
     1681 cttctagatg acaaggatcc ggtgaaaaga gacaacgtgt tgcaagccgt atgggagatg
     1741 gagaagcttg atatgcggaa gctgcaggaa gtggcggcta agtaggagtt caggagttca
     1801 ggagttcagg agttcaggag ttcaggagtt caggagttca ggagttcagg agttcaggag
     1861 ttcaggatgg ccgttgcccc aaatttcaag cctaaa
//
LOCUS       DATMKH010000435         1328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1795121, whole genome shotgun sequence.
ACCESSION   DATMKH010000435 DATMKH010000000
VERSION     DATMKH010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1795121"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1298
                     /locus_tag="VNV63_05235"
     CDS             <1..1298
                     /locus_tag="VNV63_05235"
                     /inference="COORDINATES: protein motif:HMM:NF023591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62055.1"
                     /translation="NKIPSTILSRCQHFIFTRIKRKEMIQRLAAITQADGIKVSERGL
                     AMIAKTSEGSLRDALSILDQASAYAGTAIQEEDLRQILGIVDQDLLMQLTEAILRHDA
                     AQVLDRVKLMYDQGYDSKQFFSEMVEQTRNLLVYKLAKAPQELIDLPDEELQILQRLA
                     DLAGWEQLQQLFSLFLAAQDEIRGSLYPHFTLEMTLVRASRLDVLRPIENILAQLDDL
                     QKGKPLPNPSSKQSSEHPSEQSLSDQDSFQEAQKKVHKKISPPDRQTNPPSEAPIAKS
                     AQAAALIDNPSMSSAEILKSWEATVHKIKEARPNLGSYLEQGTVIAATCEKIALGYPA
                     HAGFLTDLISKPDNRQFINETLSKIFLKPPGLEIRPGAVSVLAVKPDRDPQGQQTAKP
                     SDERPLDPVLQENLAAHPQIQEVLQIFGGQVIELKKEEV"
BASE COUNT          357 a          352 c          343 g          276 t
ORIGIN      
        1 tgaacaagat cccatctacc atcctgtccc gatgtcaaca ttttatcttt acacggatca
       61 agcggaagga gatgattcag cgattggccg ccattactca agccgatgga atcaaggtca
      121 gcgaacgggg tcttgccatg atcgccaaga cctcggaggg aagcctccga gacgctttaa
      181 gcatactgga ccaggccagc gcctatgccg gaacggcgat ccaagaggag gatctccgcc
      241 agatcctggg gatcgtggac caagacctgt tgatgcaact caccgaggcg attttgcggc
      301 atgatgccgc tcaggttctt gatcgggtca aacttatgta tgatcaggga tacgattcca
      361 agcagttctt ttccgagatg gtggagcaga ctagaaacct cttggtctat aaactcgcca
      421 aggctccgca ggagctgatc gacctcccgg atgaagaatt gcaaatcctc caacggttgg
      481 cggatcttgc cggatgggag cagcttcagc aactcttctc tttgtttctt gcggcgcagg
      541 acgaaatacg ggggagtctt tatccacatt tcacgctgga gatgacgctg gtgcgggcca
      601 gccgattaga tgtcttgcgg cccatcgaaa acatactggc ccagctcgat gacctgcaaa
      661 aagggaagcc gttacctaat ccgtcctcca agcagtcatc tgagcatcca tctgagcagt
      721 cgttaagcga tcaggacagc tttcaagagg ctcagaaaaa ggttcataaa aaaatcagcc
      781 cgccggacag gcagaccaac ccgccatcgg aggcaccgat cgctaaatca gctcaggcag
      841 cagcacttat cgacaatcct tctatgtcat cagctgagat attaaagtca tgggaagcga
      901 cggttcataa aatcaaggag gcacgcccca acctcggatc ttatcttgag caagggaccg
      961 tgatcgccgc aacatgcgag aagatcgcgc tcggctatcc tgcgcatgct gggtttctga
     1021 cggatctcat ctcaaagcct gacaaccggc aatttattaa cgagacctta tcaaaaatat
     1081 tcctgaaacc accgggtctt gaaatccgcc ccggtgcggt cagtgtctta gcagtgaaac
     1141 ccgatagaga tccacaaggg caacagactg caaagccatc ggatgagaga cctctggacc
     1201 cggtgttgca ggaaaaccta gccgctcatc cccagatcca ggaggtgctt cagatcttcg
     1261 gaggccaggt gattgaactg aaaaaggagg aagtatgacc aaccgaaaac agtttggcga
     1321 catcatga
//
LOCUS       DATMKH010000436         1050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_839670,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000436 DATMKH010000000
VERSION     DATMKH010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_839670"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(193..399)
                     /locus_tag="VNV63_05240"
     CDS             complement(193..399)
                     /locus_tag="VNV63_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62056.1"
                     /translation="MIWGLALSFIIGLPCGAFAFIIFLLKGVRPGISELKWDKKKECW
                     RDGFGFKVIEVRRFGKETGNPFLD"
     gene            444..587
                     /locus_tag="VNV63_05245"
     CDS             444..587
                     /locus_tag="VNV63_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62057.1"
                     /translation="MSPPVEVGIDGPLLPFITLPDQGLDVGAGGSSKGVIAIDQMQIL
                     RGT"
     gene            complement(574..867)
                     /locus_tag="VNV63_05250"
     CDS             complement(574..867)
                     /locus_tag="VNV63_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62058.1"
                     /translation="MIWYVVASLWQQYEINRSKLSGLPTLEFAMDIWPFDPHTKRVLL
                     LYLVQRPPAPEDALLAIHRFEKGDPYAPEAKNLERIYQTARNFELGRQWIRFP"
     gene            866..>1050
                     /locus_tag="VNV63_05255"
     CDS             866..>1050
                     /locus_tag="VNV63_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62059.1"
                     /translation="MTVIKASSRKKCQHALARRAKWEGDQSDDKEGEPDRFADEAEGE
                     ADGQAETQRPKLIGDLEP"
BASE COUNT          264 a          285 c          283 g          218 t
ORIGIN      
        1 gaactccttc gcgacctttc cagggacccc aaccttcttg gcgaactgcg ggttgtgggc
       61 agcggccgcc atcattcggg cctgcttcgg ggatttactt ggcatcagaa acctccatga
      121 tgtatctcga cgatcttcgc agtaatcaga agcccgccaa ggataagaat aacgagatag
      181 aacacaaaat catcagtcca ggaacggatt accggtttct tttccgaatc gcctgacttc
      241 gatgactttg aacccaaatc catctctcca gcattccttc ttcttgtccc attttagttc
      301 agagattcca ggccgaacgc cttttagcag gaaaatgatg aaggcaaagg ccccacaagg
      361 gagaccaata atgaagctca gggccaatcc ccagatcact ggctgatcct ttcatcagcg
      421 tagccctcga ggatactgag tgcatgagcc cgccggtcga ggtcggcatt gatggcccgc
      481 ttcttccatt catcacgctc cctgatcagg gcctggatgt tggcgctggt ggtagcagca
      541 agggcgttat agcgatagac cagatgcaga tactcagggg aacctgatcc actgccgtcc
      601 aagctcgaag ttccgagcag tttgataaat ccgttcaaga ttctttgctt caggtgcata
      661 agggtctccc ttttcaaaac ggtgaatggc gaggagagcg tcctcgggcg cgggcggacg
      721 ttggactaga tagagaagca gcactcgctt ggtatgtggg tcgaacggcc agatatccat
      781 agcgaactcg agcgtaggca gcccggagag ctttgaccga ttgatctcgt attgttgcca
      841 aagactcgcc actacatacc agatcatgac cgtgataaaa gcttccagcc gcaagaaatg
      901 ccagcacgct ctcgctaggc gtgccaagtg ggaaggagac cagagcgatg ataaggaagg
      961 cgagccagac cggttcgcgg acgaagcaga gggcgaagca gacggccaag ccgagaccca
     1021 acggcccaaa ctcataggcg atctcgagcc
//
LOCUS       DATMKH010000437         1008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1796302, whole genome shotgun sequence.
ACCESSION   DATMKH010000437 DATMKH010000000
VERSION     DATMKH010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1796302"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="VNV63_05260"
     CDS             <1..482
                     /locus_tag="VNV63_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62060.1"
                     /translation="AYRKTMFEKYGGFRIDMGPSPGSEIRNEDTEFGRRLLAAGERLR
                     YEPSAVVYHTVPEGRTNREFFLSWWFDYGRSLVREWGPGPAILRIPRPYLKILQFATI
                     VMVQRIGKWMVSTNPQQRFYEQCWIWVTAGQIKEYYRLTRVTPAKTNLPISEPRANG"
     gene            793..>1008
                     /locus_tag="VNV63_05265"
     CDS             793..>1008
                     /locus_tag="VNV63_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62061.1"
                     /translation="MNPAKKFIKAHTSKGLFDAVRDARQEWRISRLHRSALKRVGPFL
                     QRQKKKLNLGCGPNPKLGWINIDLFDSR"
BASE COUNT          243 a          247 c          281 g          237 t
ORIGIN      
        1 tggcttatcg gaaaacgatg tttgagaagt acgggggctt ccgtatcgat atgggtccca
       61 gcccggggag cgagatccgc aatgaggata ccgagtttgg ccgccggctg ttggctgctg
      121 gtgagcgctt acgatacgaa ccttctgctg tggtgtacca caccgtacct gagggcagga
      181 ccaacaggga attttttctg tcgtggtggt tcgattatgg tcggagtctg gtgcgggagt
      241 gggggccggg gccagcgatt ctacggatac ctcggcctta ccttaagatt ttgcaattcg
      301 cgactatcgt gatggtgcaa agaataggca aatggatggt ctccacgaat ccgcaacaga
      361 gattctacga gcagtgctgg atctgggtaa ccgctggaca gatcaaggaa tactatcgtc
      421 tgacccgagt aaccccggcc aagacgaatc tcccgatctc tgaacctcgc gcaaacggat
      481 agcgcatttt ggcgtgcggg ttgaaaatgc atcatggtcc cttggattaa tgccttgtcc
      541 ggttgagatg aactaattta gcagctcgga ggttttctaa tctcacattt ccaatttgaa
      601 cttcaccaac gaattgccag gcgaggtttt gttccaggaa gaagatgaga tggtcaacag
      661 aaaacgcatc ggagtcgtca tgcttgcgta taacgcggag aaaaacgctc cagaaaacgg
      721 tccaggagct ttcttgacac tctgcaccgg agtcggagct tcgctgccac accgcgtcag
      781 gaggttcgat tcttgaaccc agcgaagaag ttcatcaaag cccatacttc caaggggctt
      841 ttcgatgctg tccgtgatgc gagacaggag tggcgcatat cccggcttca ccgctccgct
      901 ttgaaaagag taggcccatt tttgcagcgc cagaaaaaga aactcaatct cggttgcggt
      961 cccaacccga aactgggatg gatcaacatc gatctatttg actctcgc
//
LOCUS       DATMKH010000438         1211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4678042, whole genome shotgun sequence.
ACCESSION   DATMKH010000438 DATMKH010000000
VERSION     DATMKH010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4678042"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..1043
                     /locus_tag="VNV63_05270"
     CDS             75..1043
                     /locus_tag="VNV63_05270"
                     /inference="COORDINATES: protein motif:HMM:NF024929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HXC62062.1"
                     /translation="MRRYYSLDGVLILFFLSTGVRSTESSEVPPKAAAFLRAYPNFFS
                     GYQDNALLFQAGGGIQFDDRRSKTYQELLSNPDPEDELSQNYPTGPQSYSAPAVNFDP
                     GRYRCAALFKKMYGESAKEVESHLTTIAWLPHSAHSSVRITRVNGVDRQLEAVSAELD
                     QLPPEEKKYVLKTGGTFNWRPIAGSEQLSAHSFGIAIDIDPDYSDYWRWSSPGANEKL
                     IPYKNRIPHRIVEIFERHGFIWGGKWYHYDTMHFEYRPELLQRESGVGGRGSCQLSVG
                     RWQKPGVTVKHPERPRTSSRKKNLRREVLLWRPSKSQNRQAEPIWT"
     gene            complement(1010..>1211)
                     /locus_tag="VNV63_05275"
     CDS             complement(1010..>1211)
                     /locus_tag="VNV63_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62063.1"
                     /translation="YAIYTQFFQSALIGNYFTIGAELGHHFTWEVQGQHLDLGYLSLG
                     LYTEFGNQYSSGHVQIGSAWRF"
BASE COUNT          286 a          309 c          343 g          273 t
ORIGIN      
        1 ctagcccgga atgcgcgtgt gcgatttacg cgctgcggga gtctagattt gaatcatttg
       61 caggccctgg atcaatgaga agatattatt cactggatgg cgtactgatt cttttcttcc
      121 tctcgaccgg cgtccgatct accgaaagca gcgaggtccc accgaaagcg gctgctttcc
      181 ttcgagcgta tcccaatttc ttctcgggtt atcaggacaa tgcacttctc ttccaggcgg
      241 ggggcgggat tcaatttgac gaccgtcggt cgaaaacgta ccaggagctt ctttccaatc
      301 cggatcctga ggacgagctc agccaaaact atcccaccgg tccgcaatcc tattcggcgc
      361 cggcggttaa ttttgaccca ggacgttacc ggtgtgccgc attgttcaag aaaatgtacg
      421 gcgagagcgc aaaggaggtg gagtcgcacc tgaccaccat tgcctggctg cctcattccg
      481 cccacagttc tgttcgcatc acccgagtca atggagtcga tcgccaactc gaagcggtct
      541 cggccgaact ggatcaactc ccgcccgagg agaagaagta cgttctcaag actgggggaa
      601 ctttcaactg gcgaccgatt gccggatccg agcaactaag cgcgcactcc tttgggatcg
      661 cgattgatat cgatccagac tattccgatt actggcgttg gagcagtccg ggagctaacg
      721 agaaactaat cccctataag aaccggattc cacaccggat cgtcgagatc ttcgagcgac
      781 atgggtttat ttggggtggt aaatggtatc attatgatac gatgcacttt gagtatcggc
      841 cggaactatt gcagagggag tcgggagtcg ggggaagggg gagttgtcag ttgtcagttg
      901 ggcgttggca gaagccggga gtcactgtca agcatccgga aagaccgaga acgagttcga
      961 ggaagaagaa cttgagacgt gaggttctgc tctggcgtcc gtctaaatct caaaatcgcc
     1021 aagcggaacc gatctggacg tgaccggaac tatactggtt cccaaattcg gtgtagaggc
     1081 cgaggctgag ataccccaaa tcgaggtgtt gaccttgcac ctcccaagtg aagtggtgtc
     1141 ccagctctgc gccaatcgtg aaatagttcc cgatgagcgc cgattggaaa aactgggtat
     1201 aaatcgcgta g
//
LOCUS       DATMKH010000439         1300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2437212, whole genome shotgun sequence.
ACCESSION   DATMKH010000439 DATMKH010000000
VERSION     DATMKH010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1300
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2437212"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /locus_tag="VNV63_05280"
     CDS             <1..901
                     /locus_tag="VNV63_05280"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HXC62064.1"
                     /translation="LFIFLETEIWPNLLRELKRHKIPSVLVNGRISEKSFKRYRLIRP
                     LLREALAQVDLFLMQTHKDAERLIALGAPQDRVVQTGNLKFDLFKVSSGPTKISDLSF
                     LNSEPVIVAGSTHEGEEAKMLDVYQQLLRSYPRLKLILAPRHPHRLEQVEGLLEASRL
                     TSGISWARRTKLDSQSGPVQVILLDTMGELKDIYQAATLVFVGGSLVPIGGHNLLEPA
                     ACGKPVLFGPYMDNFIEIAEAMKARNCGIQVQDADELGRSLKQLLDDPARREAIGQEA
                     RRWVQDEQGVVQKDMAWIEKLIG"
     gene            898..>1300
                     /gene="lpxK"
                     /locus_tag="VNV63_05285"
     CDS             898..>1300
                     /gene="lpxK"
                     /locus_tag="VNV63_05285"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HXC62065.1"
                     /translation="MNQLLIFLSWLFEMAVASRRWFYQIGLIKTRRLPARVISVGNLT
                     VGGTGKTPMVIAVAQRLKEKGYKPAVLSRGYKGKAGKGVSVVSDGEKICEDPEMVGEE
                     PILIAKRLPGIPVLVGSDRYRTGGYAFQKWGT"
BASE COUNT          309 a          289 c          375 g          327 t
ORIGIN      
        1 tctgtttatt tttcttgaga ccgagatctg gcccaacctg ctccgagagc ttaaacggca
       61 taagattccc tccgttctgg tcaatggccg gatctcggag aaatccttta agcgctatcg
      121 cctcatccgc cctctgcttc gcgaggccct ggcccaggtg gatctgttct tgatgcagac
      181 tcataaggac gccgagcgtc tgattgctct gggtgcgcct caggatcgtg tggtccaaac
      241 aggcaatctt aaattcgatc tgttcaaggt ttcatccggc ccgacgaaga tttccgatct
      301 ttctttctta aatagtgaac ccgtcatcgt tgcgggaagc acccatgagg gagaagaggc
      361 aaagatgctt gacgtctatc aacaactcct tcgttcgtac cccagattaa aattaattct
      421 ggctccgcgg catcctcatc ggcttgaaca agtcgaaggt ctcctcgaag catcgcgcct
      481 gacatcaggg atctcttggg ctagacgcac gaagctggat tctcaatcgg gaccggttca
      541 ggtgatactg ttggatacga tgggtgagct taaagatatc tatcaggccg cgactctggt
      601 ctttgtagga ggaagcttgg ttcccatagg cggtcacaat ttactggaac cggctgcttg
      661 tggtaaaccg gtgctgtttg gtccttacat ggataacttt atagaaattg cagaggcgat
      721 gaaggcaaga aattgcggta ttcaggttca ggatgcggat gagctgggcc gctctctgaa
      781 acaattgctc gatgatcctg ctcggcgcga agcgattggg caggaggcaa gacggtgggt
      841 acaggatgag cagggagtcg tacagaagga catggcctgg attgagaagt taatcgggtg
      901 aatcagttat taatttttct atcatggttg ttcgagatgg cggttgcctc aagacgatgg
      961 ttttatcaga tcggtttgat caagacaaga aggcttccgg cccgcgttat cagcgtaggg
     1021 aatcttacag taggcgggac cggcaagact ccaatggtca ttgcggttgc tcagaggctg
     1081 aaagaaaagg ggtacaaacc ggctgttttg agtcgtggtt acaagggtaa ggccggtaaa
     1141 ggtgtgagcg tggtctcgga tggagaaaag atatgcgaag acccggaaat ggtcggagaa
     1201 gaacctatct tgatcgcaaa acggttgcct ggcattccgg ttctagttgg atccgatcga
     1261 tatcgtaccg gcggttatgc cttccagaaa tggggaacgg
//
LOCUS       DATMKH010000440         4664 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4679043, whole genome shotgun sequence.
ACCESSION   DATMKH010000440 DATMKH010000000
VERSION     DATMKH010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4664
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4679043"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="VNV63_05290"
     CDS             complement(<1..446)
                     /locus_tag="VNV63_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HXC62066.1"
                     /translation="MLELAKNFRWLDLVYILLVGFIVYRLFFLRKATRTLQMMAGLAV
                     LVTALLAAHWLGLFTPDGLVTNFWSQLLLTVLVLFQPEIRRALARIGQILFNYNLTSV
                     EESQTLEEAIRAIVSLAAKKSGAIIVLEREIELKDIVEMGVPLDAKV"
     gene            complement(440..1405)
                     /gene="folP"
                     /locus_tag="VNV63_05295"
     CDS             complement(440..1405)
                     /gene="folP"
                     /locus_tag="VNV63_05295"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HXC62067.1"
                     /translation="MAQNGKAKTLRYPRRKHSSQVTPLSHRKSPSGFKILSRLAEQVR
                     GEGTEIDLSRRALIMGILNITPDSFSDGGRFLDPDQAIRHAKEMVAAGADLIDVGGES
                     TRPGAEPISSEEELRRVIPVIERLSQEIPVPVSIDTYKADVARKAIQAGARWVNDISA
                     LRFDMKMADLIAESQAPVILMHMKGTPMDMQRDPHYESVISEIYSFLGERIEFAVSAG
                     IRWEQIIIDPGIGFGKRTQDNLMILAGLERFKTLGRPILLGTSRKSFIGRVLGLPSED
                     RLEGTAATLVIGLMKGARIFRVHDVGQAVRVIRTAEAVLHEEQKC"
     gene            complement(1471..3276)
                     /gene="ftsH"
                     /locus_tag="VNV63_05300"
     CDS             complement(1471..3276)
                     /gene="ftsH"
                     /locus_tag="VNV63_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HXC62068.1"
                     /translation="MTARTKNWIFWGIVLLFMITLFNIFSVPPRPGEEELNFTQFMTS
                     LEKGDIEKVTIKDNHINGVFKSGQRKFRTYVASYPDLLKVLREKNVIIEVKPPDEAPW
                     YFTFFITYGPFVLILVFWFFLMRQMQVGGNKALSFGKSRARLLTEDRKKITFADVAGI
                     DEAKEEVVEIIEFLKDPPKFQKLGGRIPKGVLIVGPPGTGKTLLAKAIAGEAGVPFFS
                     ISGSDFVEMFVGVGASRVRDLFEQGKKNAPCIIFIDEIDAVGRLRGAGLGGGHDEREQ
                     TLNQLLVEMDGFETNEGVILIAATNRPDVLDPALLRPGRFDRQVVVSRPDLRGRNEIL
                     RVHTKRIPLDKGVNLEVIARGTPGFAGADIENLVNEAALLAARKGKKYVEMIDFEAAK
                     DKVLMGVERKSLILSDAEKKNTAYHEAGHALVAKLLPGTDPIHKVTIIPRGRALGMTM
                     QLPTDDRYSYSKDFLYNNIAILLGGRVAEELVLNHTTTGAGNDIERATDLARKMVCEW
                     GMSDKLGPLTFGKKEEEIFLGREIAKHRDYSEHTAVEIDNEVKRLVLENYERAKTIIK
                     NNINGLKAIAEALLEREVLDAPEIDKILQGAGVSA"
     gene            complement(3418..3963)
                     /gene="hpt"
                     /locus_tag="VNV63_05305"
     CDS             complement(3418..3963)
                     /gene="hpt"
                     /locus_tag="VNV63_05305"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HXC62069.1"
                     /translation="MERIFGKPIIDQEGMHKRIRDLGRQITNDYEGRDLLLVGVLKGA
                     YAFYADLARAIQLPLRVDFLVVTSSESKGKGSEKVKIVSDLTEDIKGQDVLLVEDIVD
                     SGLTVSYLKKILLAKRPRSLKVCTLLDKPNRRKVKVAVEYIGFSIPNKYVVGYGLDYQ
                     NKYRNLPYIAVLDVSDEDDAG"
     gene            complement(3967..>4664)
                     /locus_tag="VNV63_05310"
     CDS             complement(3967..>4664)
                     /locus_tag="VNV63_05310"
                     /inference="COORDINATES: protein motif:HMM:NF013821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="riboflavin kinase"
                     /protein_id="HXC62070.1"
                     /translation="KFTKPFSNQTPRQFVKKYLRQAIKAREVYAGYDFAFGKDREGRA
                     IDLKKIGEEMGFQVEIIGPVMKDGVIVSSSRIREYLQAGRAAEAARLLGRPYSIKGRV
                     RGGIGRGRKLGFPTANLFPPPEEAIPGDGVYITETMLGTNPIPSVSYIGTQPTFGQHP
                     RMVEVHLLNAGKRLYGKTLRVVFFEWLRGEMTFSSKPELISQIQRDVQQTHAYFEAQR
                     RHAQTTSAHKEKA"
BASE COUNT         1110 a         1329 c         1099 g         1126 t
ORIGIN      
        1 accttggcat cgagtggaac acccatctcg acaatgtctt tgagttcaat ctctcgttcg
       61 agaacgatga ttgcaccact cttcttggcc gcgagggaaa caatggcgcg gatggcttct
      121 tcaagggtct gggattcttc aaccgaggta aggttgtaat tgaacaggat ctgtccgatc
      181 cgagccaggg cccgtcggat ttccggttga aacaacacga gcaccgtcaa gaggagttga
      241 gaccagaagt tggtcacgag tccatccggt gtaaaaagcc ccaaccagtg tgccgcgagg
      301 agggctgtta ccaagaccgc gagcccggcc atcatttgca aggtccgcgt tgccttgcga
      361 agaaaaaaca gccggtaaac gataaacccc actagaagaa tatataccag atccagccat
      421 cggaagtttt ttgccagctc tagcacttct gctcctcatg caaaacggcc tccgccgtcc
      481 ggatcactcg aacggcctga ccgacatcat gaacacgaaa gatgcgggct cccttcatca
      541 gcccgatcac cagcgtggcg gcggtccctt ccaaacgatc ctctgaagga agacccaaga
      601 cccggccgat aaaagatttt cgcgaggtcc cgagaagaat cggccgcccg agagtcttga
      661 atcgttccag cccggctaag atcattaaat tgtcttgtgt ccgtttgcca aatccgatcc
      721 cgggatcaat gataatctgt tcccatcgaa tgccggccga gacggcaaac tcgatccgct
      781 cacctaaaaa cgaatagatt tcactgatca ccgattcata atgggggtcc cgctgcatat
      841 ccatcggtgt tcccttcatg tgcatcagga tcactggtgc ttggctctcg gcaatcaaat
      901 cggccatctt catatcaaaa cggagagcgc tgatgtcgtt gacccaacgg gcacccgctt
      961 gaatagcctt gcgggccacg tcggctttat aggtatcaat cgaaacaggg accgggatct
     1021 cctgggataa acgttcgatc acaggaataa cccgacggag ttcctcttcg gaagaaatcg
     1081 gttccgcacc cgggcgggtg gattctccgc ccacatcaat gaggtccgct ccagccgcca
     1141 ccatctcctt ggcatgacga atcgcctgat ctgggtccaa gaagcgaccc ccatccgaga
     1201 aggagtctgg cgtaatattt agtataccca taatcagcgc ccgccgggac aaatcgattt
     1261 cagtaccctc gccgcgcacc tgttccgcta agcgggatag aattttaaaa ccggatggac
     1321 ttttcctgtg agacaaaggc gtgacttgtg agctgtgctt tcgacgagga taacggaggg
     1381 ttttggcctt accattctga gccatcgagt cgttccttga aaggtaagga gcggatttaa
     1441 atcgtcagat caggaggtca aggcggtgaa tcaggcagac acaccggcac cttggaggat
     1501 cttatcaatc tctggagcat ccaagacctc cctctccaat aaggcctccg cgatcgcctt
     1561 gaggccattg atgttgtttt tgataatcgt cttggcccgt tcataatttt caagcacaag
     1621 gcgttttact tcattatcaa tttcaacagc ggtatgctcg ctgtaatcac gatgcttggc
     1681 aatctcacga cccaagaaga tttcttcttc tttcttaccg aaggtcaacg gtcccagttt
     1741 atcgctcatt ccccactcgc agaccatctt ccgggcaaga tccgtggcgc gctcaatgtc
     1801 attgccggcg cccgtggtcg tatgattaag caccagttcc tcggcaaccc ggccacccaa
     1861 gaggatcgcg atgttgttgt agagaaagtc cttagagtag ctgtaccgat catcggtggg
     1921 cagttgcatg gtcatcccca gggccctccc tcgagggata atcgtgacct tgtggatggg
     1981 atcagtcccc ggaagcagtt tggctacgag ggcatggccg gcctcatgat aggcggtatt
     2041 ctttttctcc gcatcgctca agatcaggct cttccgttcc acccccatca ggaccttgtc
     2101 ttttgccgcc tcaaaatcga tcatctcgac atatttttta cccttacgag ccgccagcaa
     2161 tgccgcttca ttcacaaggt tctcaatatc cgcgccggcg aatccagggg tgcctctcgc
     2221 gatcacctcg agattcacac ccttgtccaa cgggatccgc tttgtatgca ccctcaggat
     2281 ctcattgcgg cctcttaagt caggccgcga aacgacgacc tgccggtcaa accgacctgg
     2341 acgcaaaagg gccggatcca gaacatccgg acggttggtg gcagcgatca ggatgactcc
     2401 ttcgttggtt tcaaacccat ccatctcaac cagcaactgg ttgagggtct gttcccgctc
     2461 atcatgtccg ccacccagcc cggctccacg caatcgcccg accgcatcaa tctcatcaat
     2521 gaagatgatg cagggggcat ttttctttcc ctgctcaaac agatctcgaa cccgcgaggc
     2581 ccctactccc acgaacatct ctacaaaatc ggatccgctg atcgaaaaaa agggcacgcc
     2641 ggcctcgccc gcaattgctt tcgccaggag ggtcttcccg gtgccaggag ggcccacgat
     2701 aagaacccct ttgggtatcc gacctccaag cttttggaac ttaggcgggt ctttcaaaaa
     2761 ctcgattatc tccaccacct cttccttcgc ctcatcaatc ccagccacat ccgcgaaggt
     2821 aatctttttc cggtcttctg tgagaaggcg ggcgcggctt tttccaaagg agagggcctt
     2881 gttgccgccc acctgcatct gccgcatcaa gaagaaccag aaaaccagga tcaggacaaa
     2941 cgggccatac gtgataaaga aggtgaagta ccaaggggcc tcatcgggtg gtttcacctc
     3001 gataatgaca ttcttctcac gaaggacttt aagaaggtcc gggtagctgg ccacataggt
     3061 cctaaacttc cgttgaccgc ttttgaatac cccgttgata tgattgtcct tgatcgttac
     3121 cttctcgatg tcacccttct ccaggctggt cataaattgg gtgaagttca gctcttcttc
     3181 acctggacga ggtggaacgc taaagatatt gaacagggta atcataaaga ggaggacaat
     3241 tccccagaag atccagttct tggtacgcgc tgtcatgcac ttatctccat cacgcttttt
     3301 aattgactaa ataatcaaaa aattcataac cacgcccttg cgggcaggat ttgaaaaagt
     3361 attttgataa tatcatatct cccatgaaaa taacaatatg aaaatctaaa gggcggttta
     3421 ccctgcatca tcctcatcgg atacatccag taccgcgata taaggaagat tgcggtattt
     3481 attttgatag tcgagcccgt agcccactac atacttattg ggaatcgaga agccgatgta
     3541 ttccacagcg actttcactt ttcttcgatt tggcttatcc aacagcgtgc agaccttgag
     3601 ggatcgaggc cgtttcgcca gcaggatctt tttcagataa ctcaccgtaa gacccgagtc
     3661 cacgatgtcc tctaccagaa gaacgtcctg acctttgatg tcttcggtca gatcagagac
     3721 gattttgacc ttttcagatc ccttgccctt gctttcagaa ctcgtgacca ccaagaagtc
     3781 cacccgaagc gggagctgaa tcgcccgggc aagatccgca taaaaagcat aggccccttt
     3841 taagacaccc actaaaagga gatcgcggcc ttcataatcg tttgtgatct gtcttccaag
     3901 atctcgaatc cgcttatgca tcccctcctg gtcgatgatg ggcttgccaa aaatccgctc
     3961 catgtcttac gctttctcct tgtgcgctga agttgtttgg gcatgacggc gctgcgcctc
     4021 aaaataagca tgggtttgct gaacatcccg ctgaatctgc gagatcagct ccggcttgct
     4081 actaaaggtc atctctcctc gaagccattc aaagaagact acacggaggg tttttccgta
     4141 aagccgcttc cctgcgttta acaaatgaac ctctaccatt ctcggatgtt gaccgaaggt
     4201 gggctgggta ccgatataac tgacagacgg tatcgggttt gttcccaaca tcgtctctgt
     4261 aatatacact ccgtcgcccg gtatcgcctc ttctggaggg ggaaagaggt tggcggtggg
     4321 gaaccctagt tttcgtcccc gcccaattcc gcctcgaacc cttcccttga tcgaatacgg
     4381 cctgccaagc aatcgggctg cctctgccgc cctcccagcc tgcagatatt ctcggatgcg
     4441 agaggagctg acaatcaccc cgtctttcat gaccgggccg atgatctcca cctggaaccc
     4501 catctcttct ccgatctttt tcaaatcaat cgcccggcct tcccgatcct ttccaaaggc
     4561 aaaatcatac ccggcgtaca cctctcttgc cttgatcgcc tgacggagat acttttttac
     4621 aaactgacgc ggggtttggt ttgaaaaagg tttcgtgaat ttag
//
LOCUS       DATMKH010000441         1591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4371835, whole genome shotgun sequence.
ACCESSION   DATMKH010000441 DATMKH010000000
VERSION     DATMKH010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4371835"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            814..1305
                     /locus_tag="VNV63_05315"
     CDS             814..1305
                     /locus_tag="VNV63_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62071.1"
                     /translation="MKLTRTFIALILFSFGVGALQAQTPQPQDCGHFVQQFYDWYAAK
                     ENALMKRNSLESALEVALREKRSSFSPELVKGLKEDLAASKKSREEIVGLDFDPFLNA
                     QDVAERYLVAKITPKGDHYLVEVFGVWGGRKNRILMSFPSSPLKTANGSSRTFTTEKQ
                     TSR"
BASE COUNT          381 a          408 c          418 g          384 t
ORIGIN      
        1 gaaagttcct cattcccaat tcccgagtgt tcactcgttg caatggccgg ctgctgcggc
       61 tcgttcatcg tgaagggatt ttatccgcaa tgaatgaatt gccaatcacc aacgggagac
      121 gtaacaggat gatttgtaaa caggcttgaa gagtgttcaa cacctctggc ccgtcggttg
      181 ggagcatgtg aacccggctg gggactatta ccttgcaggc tgtaggacgc ctccgcaaag
      241 gctcattccg gcccttacgg tccgttgcaa ccttcagggc gtgatcaaag tacccttagc
      301 gtacgatttt tccgtttggt gaggtcaggc gctaatagaa gttaccgctt tggcttaacc
      361 ggacacttac cgtgctttat tttccgtttc gggtgtgcac cctctaatgg gattttagtg
      421 agtttttttg aaggccggtc tattcctcga tgagagctcc ctcgaagatc gacctcgaat
      481 caaagccggc ttgatccggc cgccagagac accatcggtc ttcatcctct tctgcaattg
      541 gtaggtgaat cagtaaatga gttctgcctt cctcccgcct ccggcaaagc ttgtcacgcc
      601 gcaggccctg tggaggcgga ccttcggcgc ggcaggccgt cttcgacctg gtaggtggga
      661 tgctctgagg ccgcttacca tgaacattga acatttcaaa aatcccgcga ggcaacgagc
      721 cttggtttct cggacgatac ggtggagtcc gcacgaaggt ttggatattg tgagaaatct
      781 gtaaatctga aaagctctag tggagaaggc tccatgaaat taactaggac atttatcgca
      841 ctcatccttt tctcctttgg cgtgggggcg ctgcaggccc aaaccccaca accccaagac
      901 tgcggccact tcgttcagca attctacgat tggtatgcgg ccaaggaaaa tgccttgatg
      961 aaacgcaaca gcctagaatc ggcgctggag gtggcgctcc gagagaaacg atcctctttc
     1021 tcccccgagt tggtgaaggg cttaaaagaa gatctagcag cctccaaaaa atcgcgtgag
     1081 gagatcgtgg ggttggattt cgatcccttt ctcaatgccc aagatgttgc tgaacgctac
     1141 ctcgtggcaa agatcactcc gaagggagat cattatttgg tcgaggtttt tggggtttgg
     1201 gggggcagaa aaaatcgaat cctgatgtcg ttcccgagct cgcctttgaa aacggccaat
     1261 ggatcttcac gaactttcac tacggaaaaa cagacatccc ggtgaacgaa aacctgctaa
     1321 gcgttctgca aattcttaaa aaagatcgac ggcaaaatgc gaagtaagga ggccatgatc
     1381 cgtcacacca tgctgtgatt cgcgtttacg atgaagccgg caacgcgatt gaaacctcga
     1441 gcaggcagcc agttatcgtc cttctattcc tcgatcaggg cactctcaaa ggtcgacttt
     1501 gaatcaaagc ctgcgtgatc gggccgccaa agattctcac cgtccgagaa aagggttagt
     1561 cgagctccgg cagactaagc ttcgccgaag g
//
LOCUS       DATMKH010000442         1646 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3707859, whole genome shotgun sequence.
ACCESSION   DATMKH010000442 DATMKH010000000
VERSION     DATMKH010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3707859"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..781)
                     /locus_tag="VNV63_05320"
     CDS             complement(<1..781)
                     /locus_tag="VNV63_05320"
                     /inference="COORDINATES: protein motif:HMM:NF040570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HXC62072.1"
                     /translation="MYRAYAIRLKTTRKQSETLNRLLAQLCELYNMALQQRRDVWRSH
                     QKGITLKEQDHQLTDLRSGCDEYWEFPRSIQRSTLKRIDLAFQGMFRRIRSGEAPGYP
                     RFKPLSQFNSFDVDLESMMVFGKSISITKIGTIKFKTRCKIKGNAKMVRISRCGGRWN
                     ARLICDIGDAPEKKSIGSVVGIDLGVSSLVTLSNGADIPNPRWERKFEGQVAAAVGRL
                     KTKKKGSKNRSKAVEAVRSVHRRISGHRREYLHSISSSLVRD"
     gene            complement(802..>1646)
                     /locus_tag="VNV63_05325"
     CDS             complement(802..>1646)
                     /locus_tag="VNV63_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62073.1"
                     /translation="KAVEADPAAYGVTVGSDPKANKAAVDARVEQMFVKAEFGIQSLA
                     DQFGKTGYEVQRDNDQLTGLMKEFGLDGHSKTSWLDQNPPTLTYPDGKTTTVGSGYMG
                     AMLHEFITKPSENAGVMAYRDTPDNPDGKSPETLDSERKFLYQFVKNHLMTQKGKQAM
                     TSDQADAFLKRTALGQPISAKQGIGPPPQAPSKQVGIPPPPQAGNRLYVGAFGVPAFG
                     APPQPGGSPQAGGQQQPAVKMYMVPGYESPVQLSEEEAAKAKAANIPIEELSPEVMNQ
                     FLGK"
BASE COUNT          342 a          474 c          376 g          454 t
ORIGIN      
        1 agtccctcac aagggatgaa cttatcgagt gcaaatactc gcgcctatga ccggagatcc
       61 ttcgatgaac ggaccgcact gcctcgacag ccttagatcg attcttactt cccttctttt
      121 ttgtctttaa ccggcccact gctgccgcaa cctgtccttc aaactttctc tcccacctcg
      181 ggtttggaat atctgctccg ttggatagag tgacaagcga ggaaacgcca agatcgattc
      241 caacaacact gccgatagat ttcttttccg gtgcatcacc gatgtcacag atcaatcgag
      301 cattccaacg tcctccacat ctggagatac gaaccatttt tgcattgccc ttgatcttac
      361 acctcgtctt gaatttgatt gttccaattt tggttatgga tatagacttc ccaaacacca
      421 tcatgctttc caaatcaaca tcgaaagaat tgaactggga taacggctta aaccgagggt
      481 atccaggcgc ctctccagac ctaatccttc tgaacatccc ctgaaaggcg aggtctattc
      541 tcttcagcgt tgatctttgt atcgatctag gaaattccca atattcatcg catccagagc
      601 gcaggtctgt caattgatgg tcttgttctt ttaaggtgat tcccttttgg tgagacctcc
      661 acacgtctcg cctctgctgg agagccatgt tatacaactc acacaattgc gcaagaagac
      721 gattaagcgt ttcgctctgc tttctcgtcg tcttcagacg tatggcgtat gcgcggtaca
      781 tggcttatcc ccattttatc ttcatttacc tagaaattga ttcattactt ctggactcaa
      841 ttcctcaata ggaatgttcg cggcttttgc cttcgccgct tcctcttccg acaattgcac
      901 cggggattcg tacccaggga ccatgtacat ctttacggct ggctgctgct ggccaccggc
      961 ctgcggagat cctcctggct gcggcggagc gccaaacgcg ggaactccga atgccccgac
     1021 gtataatctg tttcccgctt gcggcggagg cggtattcca acctgcttcg acggagcctg
     1081 cggaggcggt ccaattccct gcttggccga tataggctgt cccagcgcgg tccgcttcag
     1141 aaaagcatcc gcctggtcac tcgtcatcgc ctgctttccc ttctgggtca tcaggtgatt
     1201 cttgacgaat tgatacagga acttgcgctc ggaatccaga gtttcaggag acttcccatc
     1261 gggattatcc ggagtgtccc ggtaggccat gacaccagcg ttttcgcttg gctttgtgat
     1321 gaactcgtgg agcatggcgc ccatgtatcc actgcctacc gtagtcgtct ttccgtctgg
     1381 gtaagtcaga gtcggagggt tctgatcgag ccaactggtc tttgagtggc cgtcaagccc
     1441 gaactccttc atcaagcccg taagctggtc gttgtctctc tgaacttcgt accctgtctt
     1501 tccaaactga tccgcgagag actggatgcc gaattccgcc ttgacgaaca tctgctctac
     1561 gcgagcatca actgccgcct tgttcgcttt ggggtcactt ccaacggtca cgccatatgc
     1621 ggccgggtca gcttccacag ctttct
//
LOCUS       DATMKH010000443         3257 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_843425,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000443 DATMKH010000000
VERSION     DATMKH010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_843425"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1970)
                     /locus_tag="VNV63_05330"
     CDS             complement(<1..1970)
                     /locus_tag="VNV63_05330"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HXC62074.1"
                     /translation="MIGSGMGIGMGMGRGILTFILVIFLEALAIGEEPSQYPMHGQME
                     MSGQMSAAEQQFYQDRAKGKTDAVKTPAGVQYQGAGPAMLNALTEHLDKEIANAGGNL
                     GGYASGAEAHAMSQGIPLLISDGEAVTQGGRCPSGVPVKTYDISAINIQITLNRFLDY
                     FPGYLYVLTEDVAKVRAEEKKNKDARKSNDPYPAAGVSIGLQGDLLQPLVIRANAGDC
                     VRITLRNQLGELKEDGSVKIDKDGDPVSIHIHGSSFIVRSTGQAATMTNKDTLVKPGN
                     SQEFEWYIPGDEPDKAHHFHSHAVREQWSQGMFGALVVEPRGSRYLSPWTGQELKSGW
                     MAMIQGSGSPDFREFAIFYHEIGDEVFRINKKKDGELLDQRDALTDTYRPGGRGLNLR
                     SEPHGTRLALQQERHGIHDESQGYGSYTFGDPATTVPRSYLGDPTKFRMMGGAEVIHS
                     HHLHGGSDRWPRSPEAVTKAEFNFAATANGTGPVKFPPIRTSSERLDVQAIGAGESYN
                     QVIECGSGGCAYGAGDYTFHCHIPQHYVTGMWGFWRVYNTLQTAGSQDDLMPVLAELP
                     DRKGKMKPAVDSSKLIGTTVDWFGGKKFKITADKTDWKSSPAMISIKDWVEMQLPAQG
                     LPGGQTDEHKQIVSYDASVFDWKWDGQTALNEP"
     gene            complement(2331..>3257)
                     /locus_tag="VNV63_05335"
     CDS             complement(2331..>3257)
                     /locus_tag="VNV63_05335"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HXC62075.1"
                     /translation="SSNTTILIYGESGTGKELVARAIHQCSTRRHRTFIPVDCGALPE
                     TLLESELFGHVKGAFTGAIQNKKGLFEEAEGGTLFLDEIGDTSTAFQSKLLRALQEGE
                     IRPVGGNQRIKVNVRVVAATNKLLKEAVAKKMFREDLYYRLAVMPIVVPPLRERPDDI
                     LPLARYFIQKYAGHSGKGEMHLSEDAADLLLRLLWRGNIRELENVIERAVLVSPGNLI
                     AAGSLLIEEEIIAPDDLSSAPLSEPISVPLFVAIKQICSRAEWERIADAIKKNNGNKS
                     LAARSLGIARSSLYNKIKRYKIEKQNHLNPGF"
BASE COUNT          694 a          900 c          839 g          824 t
ORIGIN      
        1 ggttcgttaa gagcggtttg gccgtcccat ttccagtcga agacggaggc atcgtaggag
       61 acaatctgtt tgtgctcatc tgtctggccg cctggcagac cctgtgccgg aagctgcatc
      121 tcgacccagt cctttatgga aatcatcgcc ggactgctct tccaatcagt tttatcggcc
      181 gtgatcttga atttctttcc gccgaaccaa tccactgtcg tgccgatgag cttggacgaa
      241 tcgaccgccg gcttcatctt gcctttgcga tccggcagct cggctagcac cggcatgagg
      301 tcatcctgcg aaccggccgt ttggagggtg ttgtaaaccc gccagaagcc ccacatgccc
      361 gtgacataat gttgggggat atggcagtgg aatgtgtaat cgcctgcgcc gtaggcacaa
      421 ccgcccgagc cgcactcgat cacttggtta taagattcgc ctgcgccgat cgcctggacg
      481 tctaaacgct cggaactggt acgaataggc gggaacttca ccggcccggt tccgttggcg
      541 gttgcggcga aattaaactc ggccttcgtg acggcctccg gggaacgcgg ccatcggtcg
      601 ctgccgccgt gaaggtggtg ggaatggatg acttcggcgc cgcccatcat acgaaacttt
      661 gtcggatcgc cgagatatga tcgcggtacc gtcgtcgcgg gatcgccgaa ggtataggac
      721 ccgtatccct gcgattcgtc atgaatgcca tgacgttctt gttgcagtgc caggcgcgtt
      781 ccgtggggct cgcttcggag attcaacccg cggccgccgg ggcgataggt atcggtcaga
      841 gcatcccgtt gatcgagcag ctccccgtcc ttctttttat taatccggaa aacctcatcg
      901 ccgatttcat gatagaaaat cgcgaactct cgaaaatcag gactgccgga gccttgaatc
      961 attgccatcc aaccgctctt caattcctgt ccggtccagg ggcttagata gcgggaacct
     1021 cgaggctcga ccaccaatgc gccgaacatc ccttgggacc actgctcacg caccgcatgg
     1081 ctgtggaaat ggtgtgcctt gtccggttca tcacccggaa tataccattc gaactcctgg
     1141 ctgtttcccg gtttgaccag cgtgtcttta tttgtcatcg ttgcagcttg tcctgtggac
     1201 cgcacaatga agctggaacc atggatatgg atgctgaccg ggtcgccgtc tttgtcgatc
     1261 ttaactgagc cgtcttcctt caattcgcct agctgatttc tcaaggtgat acgaacacag
     1321 tcgcccgcat tcgcgcggat cacaagaggt tgaagaagat cgccctgcaa tccaatagag
     1381 acgcctgcag ccgggtatgg atcattgctt tttcgggcat ccttgttttt cttctcttcg
     1441 gctcgaacct tcgcaacatc ttctgtcaac acatagagat atccggggaa gtaatctaaa
     1501 aagcgattaa gtgtgatctg gatattgatt gccgaaatgt cgtaggtttt gaccggcacc
     1561 ccggacggac aacgtcctcc ctgagtcacg gcttctccat ctgaaatgag aagcggtatc
     1621 ccctgcgaca tcgcgtgggc ttcggcgccg gaggcatatc ctccgagatt tccgcccgca
     1681 ttcgcgatct ctttatcaag gtgctctgtc aaggcattca gcattgccgg accggccccc
     1741 tgatattgaa ccccggccgg ggtctttacc gcgtcggttt ttccttttgc ccgatcctga
     1801 tagaactgct gctctgcggc gctcatctgt cctgacattt ccatctggcc gtgcatagga
     1861 tattgcgatg gctcttcgcc aatggccaag gcctccagga aaatgaccag aataaaagtc
     1921 agtattcctc ttcccatacc cattccgata cccatgccac ttccgatcat cgtgtctata
     1981 cctcccgtta tgttatcccg cacactcttt caattttatt ttgagcattc gtttccccca
     2041 gaagattctc aagctgcatc ggaacctaag acgcttgatt cgcttctggt aaagaaacag
     2101 ataaatagac agcaagagac atgccatgaa cgtcaattcg agtggagaag ttatatgcca
     2161 gaaaaataaa gagaaaccgg gccccacttc atttttctaa aggctgaatc gtccagtaag
     2221 ttggacagat gtttattata ttgtacggtt gtttaaaaca gaggacagtt tgccggatga
     2281 actgagagac actttgtttc ggggatgttt gcttgaacgg ctaatttgga tcagaagccg
     2341 ggattgagat ggttctgttt ttcgatcttg taccgtttga tcttattata cagactggat
     2401 cgcgcgatcc cgagggaacg ggccgccaac gatttattcc cgttgttttt cttgatcgca
     2461 tcagcgatcc tttcccactc tgctcttgaa cagatctgct tgatcgcgac gaacagagga
     2521 acggagatcg gctctgagag gggagccgag gaaagatcgt ccggagcaat aatttcttct
     2581 tcgatcaaga gcgatccggc agcgattaag tttcccggag agaccagcac agcccgttcg
     2641 atcacgtttt ccaactcacg aatgttccct ctccagagca gccgaaggag gagatcggcg
     2701 gcatcttccg aaagatgcat ttcccctttc ccgctatgtc ctgcgtactt ttggataaaa
     2761 tatcgggcta gggggagtat atcgtcagga cgctcgcgta gtggaggaac cacaataggc
     2821 atcaccgcaa gccggtagta gagatcttcc ctgaacattt ttttcgcgac cgcttccttc
     2881 aggagcttgt tggtggcggc caccacgcgt acgttgactt ttatacgctg gtttcccccg
     2941 accggacgga tctcgccttc ttgcagcgcg cggagcaact ttgactgaaa tgccgtgctg
     3001 gtatcgccga tctcgtccag gaagagggtc cctccttcgg cctcttcaaa aaggcctttt
     3061 ttgttttgaa tcgcccctgt aaaggctcct ttgacatggc cgaacagctc gctctccaag
     3121 agcgtttctg gaagcgcacc gcaatccacc ggaataaatg tgcggtgccg gcgggtactg
     3181 cactgatgga tcgcccgagc gaccaactcc ttcccggttc cgctctcacc gtaaatcagg
     3241 atcgtggtgt tactgga
//
LOCUS       DATMKH010000444         3833 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2438871, whole genome shotgun sequence.
ACCESSION   DATMKH010000444 DATMKH010000000
VERSION     DATMKH010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3833
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2438871"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..879)
                     /locus_tag="VNV63_05340"
     CDS             complement(<1..879)
                     /locus_tag="VNV63_05340"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXC62076.1"
                     /translation="MHSVLIVDDEKVEAGILGEILTHEGFDVTTQTDPEQAAALVQKQ
                     SFDLVLCDLKMPKIDGIELLQQLRRIHPDLTVIIMTAYGTIETAVNAMRQGAFDYVTK
                     PFSKEQLLITVQRAIKNLEILRENVYLKEELGLHQALIPIVGKSESIQNIYGMIAKIV
                     QDENATVLIQGETGTGKDMVARAIHQMSPRRDAPFIVVNCAAIPSGLMESEFFGHEKG
                     AFTGATQAKPGRFELADRGTLFLDEVSELEADLQGKLLRVLQTKEFERVGGIRTQRVN
                     VRVIATSNKILQEEVRI"
     gene            complement(872..2389)
                     /locus_tag="VNV63_05345"
     CDS             complement(872..2389)
                     /locus_tag="VNV63_05345"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXC62077.1"
                     /translation="MNPFGSYRQRLTWVMLLLMFTTTGAFFFINYQIEKKILIDQKLQ
                     RAVLMGKTLRINLSQLILKSSPRDFASIPEDQKEQIRQFIEHFDAEQRPIDKYSQNEW
                     FHDLFFIDSSSKVIIDYPAQKEGRTLPPEERIDRDTLTKLEKNEIDTQIRRRGKEQML
                     FLTFPLFHKEQLLGFGRIEMSLDSVMEFLGQIKLWSLVTAWCLFLVGFLFASYFARTV
                     TKPVEELVQAAVRIGQGDLTQRLDESKKDEIGLLKIAFNRMAEGILKLEEAQKRVEKL
                     EVAGLLGARVAHEIKNPLNSIGLIIDHFKDRFIPKEKAAADKFLELSENMKLELERLN
                     EIVEGFLRFAKPAVLSRQPTNPNDLIDGTVAVITPEADKQGVQIHRHFDSAVPKIWVD
                     YHSMKQALLNLLINALQAMPEGGELHLFTSIGDHGAEEVVISVRDTGCGIPKENLPKL
                     FDPYYTTKARGFGLGLSIVERIAQEHGGRIEVKSELGKGATFTLFFPVKVRGQHA"
     gene            complement(2467..>3833)
                     /gene="uvrC"
                     /locus_tag="VNV63_05350"
     CDS             complement(2467..>3833)
                     /gene="uvrC"
                     /locus_tag="VNV63_05350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HXC62078.1"
                     /translation="PNCHIVIDGTAERACIEYEIKRCLAPCTGQQSKEDYQAMMRQVR
                     LFLEGKDTELLKGLKAQMEIESARLNFEEAARLRDQIANIQRVLEKQRITTTELVDQD
                     VIALAREGAAVDLQIFFIRGGMLVGRKDFFWADIGETSEEDLLVSFIQQFYDQEVLVP
                     KEILLPLKLQETELEVLGSWLTERKGETVYLRTPIRGKDSHLIGLAQENARLKLSEHL
                     QEQNAKTTEAILLRQLLGLKKVPQRIECFDISNIGGEQAVGSMVVWEGKRAKKSDYRH
                     FKIRTVQGANDFAMLQEVVSRHYTRVKSEGRLLPDLIVIDGGKGQLSAVQEVLQGLGL
                     SEIDLIGLAKARGEKEERVFLPGHSESIPLPPTSPATHLLQRIRDESHRFAITYHRKL
                     RGKDMITSVLDEVSGIGEARKRALLIRFGGLDGIKAASLEELSAVPRMNKKLVETLYR
                     VLHQ"
BASE COUNT          855 a         1062 c          933 g          983 t
ORIGIN      
        1 gatgcgcacc tcctcctgaa ggattttatt gcttgtggct atgacccgga cattgacccg
       61 ttgagttcgt atcccaccaa cccgctcaaa ctcctttgtc tgaagcacac gaagcagctt
      121 cccctggaga tcagcctcca actcggacac ttcgtccaga aacagtgttc cacggtcggc
      181 cagttcaaaa cgtcccggct tggcctgtgt tgcaccggtg aaagccccct tctcgtgtcc
      241 gaaaaattcg ctttccatca atcccgacgg aatggcggca cagttcacaa cgataaaggg
      301 ggcatccctt cgagggctca tctgatgaat agctcgggct accatgtcct tgcctgttcc
      361 ggtttcccct tgaatgagaa ccgttgcgtt ctcatcttga acgatcttgg cgatcattcc
      421 gtagatattc tggatggact cgctcttccc gacaattgga atcaacgcct gatgaaggcc
      481 cagctcctcc ttgaggtaaa cgttttcccg gaggatttcg agattcttga tggccctttg
      541 aaccgtgatg agaagttgtt cctttgaaaa gggcttggtc acataatcaa aggccccttg
      601 gcgcatcgcg ttcacggcgg tctcgatggt tccgtaggcg gtcataatga tcacggtaag
      661 atcgggatga atcctgcgaa gctgctgcag gagttcgatc ccatctattt tgggcatctt
      721 caaatcacag agcaccagat caaaagactg cttttgaacc aaggccgccg cttgctccgg
      781 gtcggtctgg gtggtaacgt caaacccttc gtgggtcaag atttctccaa gaatgccggc
      841 ctcaaccttc tcatcgtcta cgatcaaaac actatgcatg ttgtcctctg acttttaccg
      901 gaaagaagag ggtaaaggtc gcccccttgc ccaactcact tttgacctca atccttcccc
      961 catgctcttg agcaatccgc tcgacgattg aaagcccgag tccgaaccca cgggctttgg
     1021 tcgtgtagta gggatcgaac agcttgggaa ggttctcttt tggtattccg catccggtat
     1081 cgcgtacgga gattacaacc tcttccgccc catgatcacc tattgatgtg aagaggtgga
     1141 gttctccacc ttccggcatg gcctggaggg cgttgatcaa taggttgaga agagcctgct
     1201 tcatggaatg ataatccacc cagattttag gaacagccga atcaaaatgc cggtggattt
     1261 ggaccccctg cttgtctgcc tccggagtga tcacggcgac ggttccatca atcagatcgt
     1321 ttgggttcgt cggctggcgc gaaagaacag cgggcttggc gaatcttaag aagccttcga
     1381 cgatttcatt gagtctctct aactccagct tcatattttc ggacagctcc agaaatttgt
     1441 cagcggcggc cttttccttc ggtataaacc ggtccttgaa gtgatcaatg atgagaccga
     1501 tgctatttaa gggattctta atctcgtgag ccacacgggc gccaagcagg cctgcgacct
     1561 ccagcttttc gactcgtttc tgcgcttcct ctaacttgag gattccttcc gccatccggt
     1621 tgaaggcaat ctttaagagc ccgatctcat ccttcttgga ctcatccaga cgttgggtga
     1681 gatcgccctg accgatccgg acggccgcct ggaccaactc ttcgaccggc ttggtaacag
     1741 ttcgagcaaa ataggaggca aaaagaaatc cgaccagaaa aagacaccaa gccgtgacca
     1801 ggctccatag cttgatttgg cccagaaatt ccataacgga gtccagagac atttcgatcc
     1861 taccaaagcc taagagttgt tccttgtgga agagaggaaa ggtcaggaaa agcatttgct
     1921 cctttccacg tcggcgtatt tgtgtatcaa tctcgttttt ttcaagtttg gttaaggtgt
     1981 cgcggtcgat cctctcttcc gggggaaggg ttcgaccctc tttttgcgca gggtagtcaa
     2041 tgatgacctt gctgctcgaa tcaatgaaaa agagatcgtg gaaccattcg ttctgactgt
     2101 atttatcaat cggcctttgc tccgcatcga aatgttcgat aaactgccgg atctgctctt
     2161 tttgatcttc cggaatactc gcaaagtcac gcgggcttga ttttaaaatg agctgtgata
     2221 gattgatccg taacgtcttg cccatgagca ctgcacgctg gagtttctga tcaattaaga
     2281 ttttcttctc gatctggtag ttgataaaga aaaatgcccc ggtcgtggta aacatcagca
     2341 ggagcattac ccatgtcagg cgttgtcggt atgatccaaa ggggttcatc aagtctattc
     2401 tattacatgc tgcattccag acgcaagata tgggggtcct ggtttatttt taggtaaagg
     2461 gctttgctat tgatgcagca cccgatagag cgtttcgacg agttttttgt tcattcgggg
     2521 cacggcacta agttcttcga gtgaggccgc cttgatcccg tcgaggccgc cgaatcggat
     2581 caggagcgca cgctttcgcg cctccccgat cccggaaacc tcatccagga ccgacgttat
     2641 catgtctttc ccgcggagct tccgatgata cgtgatcgca aaccggtgcg attcatcccg
     2701 aatccgttgc aaaagatggg tggcaggcga agtcggcgga agcgggatag actccgaatg
     2761 tcccggtaga aagacgcgct cctctttctc gccgcgggcc ttggccagcc cgatcagatc
     2821 aatctccgag agacccagac cttgcaatac ctcctgtacc gcactaagct gacccttgcc
     2881 gccgtcaatc acgatcagat cgggcaagag gcggccttct gatttcaccc gcgtgtaatg
     2941 acgactcacg acttcttgaa gcatcgcgaa atcattggcc ccctgaaccg tccggatctt
     3001 aaaatgccga tagtccgact tcttagctcg tttgccttcc cagaccacca tcgagcccac
     3061 cgcctgctct cccccgatgt tggagatgtc gaagcattca atacgctgcg ggaccttctt
     3121 caatcccaag agctgccgaa gcaggatcgc ctcggtcgtc tttgcgttct gctcctgaag
     3181 atgctcgctc aatttgagcc tcgcattctc ctgagccagt ccaataagat ggctgtcctt
     3241 tccccgtatg ggtgtccgga gatagaccgt ctctcctttt cgctcagtca accaggatcc
     3301 cagcacctca agctcggttt cttgaagctt caagggaagc agaatctctt ttgggacaag
     3361 aacttcctgg tcgtaaaatt gctgtataaa cgacaccaga agatcttcct ctgaagtctc
     3421 tccgatatca gcccagaaga aatcctttcg acctaccagc atcccgccac gtatgaagaa
     3481 gatctgaaga tccaccgcgg cgccttctct cgcaagcgcg attacatcct gatccaccag
     3541 ctccgtggtg gtgatccgtt gtttttcgag gaccctttga atattggcga tctgatcccg
     3601 cagccgggcc gcttcctcga agttcaggcg agccgattcg atctccatct gagccttcag
     3661 gcctttcaac agttcggtgt ccttgccttc cagaaacagc cgcacctggc gcatcatcgc
     3721 ttgataatct tctttagact gttgaccggt gcagggggcg aggcagcgtt tgatttcata
     3781 ctcgatgcag gcccgctccg ccgtcccatc aatcacgata tgacaattgg gaa
//
LOCUS       DATMKH010000445         1587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_521104,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000445 DATMKH010000000
VERSION     DATMKH010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521104"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="VNV63_05355"
     CDS             complement(<1..384)
                     /locus_tag="VNV63_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HXC62079.1"
                     /translation="MDADEQLRTPLFDAMVGLAEARKVSFHTPGHKSGKGISTRFRKF
                     VGPKIFSIDLTTLDEVDSLQKPKGVIKEAQELAAKAYGADRSYFLVNGTSGGNQAMIL
                     TTCNPGDKVLVGRNAHKSVLAGLIAT"
     gene            complement(388..1311)
                     /gene="speE"
                     /locus_tag="VNV63_05360"
     CDS             complement(388..1311)
                     /gene="speE"
                     /locus_tag="VNV63_05360"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HXC62080.1"
                     /translation="MPEQQKSYKWFLDLLNPEEGHMHGITTILYAKQTKYQQMEIMET
                     KSYGRCLVLDGKMQSSEADEFIYHEALVHPALLTHPNPQKVFVVGGGEGATLREILRH
                     KSVQSTLMVDIDQEVVESCKKYLPEWHQGAFDDKRVALKFLDARKFLEETQETYDIII
                     IDISEPVEEGPAYLLYTKEFYQIVNKRLTKDGIISLQAGTTSLTALLNFTAVYQTLKT
                     AFPIVAGYQACIPSFGLPWGFALASKKHDPRKLTPEEVDKRIKERVTGELRYYDGETH
                     QGQFLLPKHVRREVEAENQIIEDNHPLFTFH"
     gene            complement(1274..>1587)
                     /gene="speD"
                     /locus_tag="VNV63_05365"
     CDS             complement(1274..>1587)
                     /gene="speD"
                     /locus_tag="VNV63_05365"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HXC62081.1"
                     /translation="IETSFHRFSPFGISGVVVIAESHLTIHTWPEYGYAAVDIFTCGD
                     LLQPNIAAAYLIEKFQSKNPSIFEMKRGLLARGDVKLPHKAIKEVSDYARTTKELQMV
                     P"
BASE COUNT          330 a          397 c          444 g          416 t
ORIGIN      
        1 ggttgcgatg agtccggcca gaacggactt gtgggcattg cgtcccacaa ggaccttgtc
       61 ccccggattg caggtggtaa ggatcatggc ctgattgccg cccgatgttc cattcacgag
      121 aaagtaggag cgatccgcgc cataggcctt ggccgcgagc tcttgggcct ctttgatgac
      181 gcctttgggt ttctgtaggg agtccacctc atccaaggtg gtgagatcga tcgagaagat
      241 cttgggacct acgaatttcc ggaaccgggt tgagatcccc ttaccgcttt tatgaccggg
      301 cgtgtggaac gaaaccttgc gggcctcggc cagtcccacc atcgcatcga aaagcggggt
      361 cctcagttgt tcatcagcgt ccatcgatta gtggaaggta aacagcgggt ggttatcctc
      421 aatgatctgg ttttctgctt caacttcgcg gcggacgtgc tttggcagaa gaaactgccc
      481 ctgatgagtt tccccgtcat agtatcggag ctctccggtg actcgttcct taatccgctt
      541 gtccacctct tcaggggtta gttttcgggg atcatgtttt ttggaggcca gcgcgaaacc
      601 ccaggggagg ccaaaagagg gaatgcaagc ttgatagcct gcaacgatcg ggaaagcggt
      661 cttcagcgtc tgatagactg cggtgaagtt gagcaaggcg gtcagcgaag tggtcccggc
      721 ctgaagcgag atgatcccat ctttcgtaag gcgcttattg acgatctggt aaaattcttt
      781 cgtgtagagg agataggccg ggccttcctc gaccggttcg gagatgtcga tgatgatgat
      841 gtcgtacgtc tcctgagttt cttccaggaa tttccgggca tccaggaatt tcaaagccac
      901 acgcttgtcg tcaaaagccc cctggtgcca ctcgggtaga tacttcttgc agctttccac
      961 cacctcttgg tcgatgtcca ccatgagggt ggactggacc gatttgtggc ggaggatttc
     1021 tctgagcgtc gccccttctc ctcctcccac aacaaagacc ttttgaggat tagggtgggt
     1081 cagcagggcc gggtgaacga gcgcttcatg gtagatgaat tcatctgcct cactggactg
     1141 catctttccg tccaggacaa ggcaccgacc atagcttttg gtttccatga tctccatctg
     1201 ctgatacttc gtctgtttgg catagaggat cgtggtgatc ccatgcatat gcccttcttc
     1261 cgggttcagc agatcaagga accatttgta actcttttgt tgttctggca tagtcggaca
     1321 cctctttgat cgctttatga gggagcttaa cgtctcccct tgcaagcagc cctctcttca
     1381 tctcgaagat cgagggattt tttgactgaa atttttctat aagataagcg gcagcgatat
     1441 tgggttgaag gagatcgccg caggtgaaga tgtctacggc ggcgtatccg tattcgggcc
     1501 aggtatgaat cgttaagtgg gattccgcaa tgactaccac accgctgatt ccaaaggggc
     1561 taaatctgtg gaaggatgtt tcgatga
//
LOCUS       DATMKH010000446         4270 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_521449,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000446 DATMKH010000000
VERSION     DATMKH010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4270
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521449"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="VNV63_05370"
     CDS             <1..484
                     /locus_tag="VNV63_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62082.1"
                     /translation="IDNDKTVYLGTERGLFKREEGGSGWIVVEAPSGGGPLVTSALLW
                     TEEHGLFAAGPTGLFLGKRQNNRSFAWQAKNRGLPSNGIVQVLAQDLKHPSLIYAGTS
                     KGLFLSQNGGDQWEPARITGEDASLLDIRSIAIQPDGMLYVGTTDAGVLVGINRLSPS
                     "
     gene            complement(498..809)
                     /locus_tag="VNV63_05375"
     CDS             complement(498..809)
                     /locus_tag="VNV63_05375"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HXC62083.1"
                     /translation="MTAKALREKRRYRRVKILQDIYFGHRGQRRVDDLSEEGMFIATP
                     ASYLPGSDLDLKFRLFGDDLPVEVQAEVRYVQKGIGMGVRFLGLKQKDRKRIREFVKR
                     F"
     gene            complement(812..1318)
                     /locus_tag="VNV63_05380"
     CDS             complement(812..1318)
                     /locus_tag="VNV63_05380"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HXC62084.1"
                     /translation="MLSIPKQFYDAMIQQAQSEHPNECCGLLAGKDGGVSQLYKIKNI
                     VALEGLEAANFDDAKLAHLKSLSPEDRAEIAFAMDAREMSSAFKDMRLNDIKLQVVYH
                     SHPHGPNRPSVTDIKNAIGFDSMREKLNLPEPLHIIISLQKRNQPEVKAYRIVEDQTT
                     LEEFKIVG"
     gene            complement(1320..2123)
                     /gene="moeB"
                     /locus_tag="VNV63_05385"
     CDS             complement(1320..2123)
                     /gene="moeB"
                     /locus_tag="VNV63_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010880850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HXC62085.1"
                     /translation="MMNFTDEEIQRYSRQIILPEIGGKGQKKLRNARLFLVGAGGLGS
                     PAALYLVAGGLGKLGLIDSDVVDLSNLQRQVLHTTKTINQPKVESAKARLKAINPDCN
                     IETYQEKLSASNIMELLSDYDLILDGSDNFPTRFLVNDACYFLKKPLISASIFRFEGQ
                     LTTIKAYADHPCYRCLFPEPPPADLVTSCQEAGVLGALAGTLGVLQATEALKEILGIG
                     SSLTGRLVLYDALEMRFREVKIHKDPNCPLCGPHPKITKLMEYEATCRL"
     gene            complement(2129..2368)
                     /locus_tag="VNV63_05390"
     CDS             complement(2129..2368)
                     /locus_tag="VNV63_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIL domain-containing protein"
                     /protein_id="HXC62086.1"
                     /translation="MASLKFHITFPEGKIKEPIIYQIGKEYKVVTNIRKADVTEKTGW
                     VDLELTGDPKEIERAVVGMKNKGVKVDPIERNIIE"
     gene            complement(2372..2650)
                     /locus_tag="VNV63_05395"
     CDS             complement(2372..2650)
                     /locus_tag="VNV63_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HXC62087.1"
                     /translation="MAFKVRIPSPLRPLTAGQGQVDGVGSNIKDLIENLEKKFPGMKT
                     RLYDETGNLRRFVNIYVNEEDIRFLKGEDTTLKEGDEISIVPAIAGGC"
     gene            complement(2640..3920)
                     /gene="thrC"
                     /locus_tag="VNV63_05400"
     CDS             complement(2640..3920)
                     /gene="thrC"
                     /locus_tag="VNV63_05400"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HXC62088.1"
                     /translation="MTNNKMKGLKCRECGKVYPTDPLHVCEFCFGPLEVQYNYDEIKK
                     QISREAISRGPNSLWRYIDLLPVEGPATVGLHAGLTPMIRATRLAKRLGLNELYLKND
                     TVNHPTLSFKDRVVAVALTRAKEFGYDTVACASTGNLANSVAAHAAQAGLNCYVFIPS
                     DLESAKILGNLIYKPTVVAVEGNYDDVNRLCSEIAAEYNWAFVNINIRPYYAEGSKTL
                     AYETAEQLGWRAPDQVVVPVASGSLLTKIWKGLKEFETLGIIDSAKTRINGAQAEGCS
                     PVATAFREKRDFIKPVKPMTIAKSLAIGNPADGYYALKTTKESGGVFETVTDAEIIEG
                     IQLLAETEGVFAETAGGVTVGVLKKLVQGGRIKPKEVVVAYITGNGLKTQEAVMDAVG
                     SPVRLEPSLAAFEEAFKPNKTGTALSGKGSSHGV"
     gene            complement(3937..>4270)
                     /locus_tag="VNV63_05405"
     CDS             complement(3937..>4270)
                     /locus_tag="VNV63_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009371445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HXC62089.1"
                     /translation="DGQAMTILPKQTTCYRCIFKNPPPQGLIPSCQEAGVLGALAGLI
                     GTIQATEALKLILGIGNLLTDRLLTYDALGTRFREVKLRRNPKCPVCSEHPQITELMD
                     YEQEACRI"
BASE COUNT          878 a         1141 c         1157 g         1094 t
ORIGIN      
        1 catcgacaat gacaagacgg tttatctggg aacggagcgg ggtcttttta agcgggaaga
       61 aggcggctca ggctggatcg tggtggaagc tccttccggt ggcggcccgc ttgtgacaag
      121 cgccctcctc tggacagaag aacacggctt gtttgcggcc gggccaaccg gtctttttct
      181 cggaaagcgg cagaacaacc ggagtttcgc atggcaggcg aagaatcgtg gattgccatc
      241 aaacggcatc gtccaagttc tggcgcaaga cctaaagcat ccgtctttga tttatgcggg
      301 gaccagcaag ggactctttt taagccagaa cggcggggat cagtgggagc cggcgcgcat
      361 caccggagaa gatgccagct tgcttgatat tcgttctatt gcgatccagc cggacggtat
      421 gctgtatgtc gggaccacgg atgccggcgt cctggtcggc atcaaccggc tgtcaccgag
      481 ttaaaaaata cgttccctta gaaccgcttc acgaattccc gtatccgctt gcggtctttc
      541 tgcttgaggc cgaggaatcg tactcccatg ccgattcctt tttgcacata ccggacctcg
      601 gcctgaacct cgaccgggag atcgtcgcca aaaagccgaa atttaagatc caggtcggat
      661 cctggaagat agctggccgg tgtggcaatg aacatccctt cctcggagag atcgtccacc
      721 cgtcgctggc cccgatggcc aaaataaata tcctgcagga ttttcacccg tcggtaacgc
      781 cgtttttctc ttagagcctt agcggtcatt tctatcctac tatcttgaat tcttccagcg
      841 tggtttggtc ttctacaatc cggtaagctt ttacttccgg ctggttccgc ttttgaagag
      901 aaataatgat gtgaagcggt tcagggaggt ttaatttttc tcgcatggag tcaaatccga
      961 ttgcattttt aatgtcggtc actgaagggc ggttggggcc gtgcggatga gaatgataga
     1021 cgacctgtaa cttaatgtcg ttcaatcgca tgtctttgaa tgcagaggac atctctcttg
     1081 catccatggc gaatgcgatc tcagcgcgat cttccggaga gagactcttg agatgggcaa
     1141 gttttgcatc atcgaaattg gcggcttcca acccttccaa ggctacaatg tttttgatct
     1201 tataaagctg gctcaccccg ccgtcttttc cggccaagag accgcagcac tcattcggat
     1261 gctcagactg ggcctgctgg atcattgcat cataaaactg tttggggatc gaaagcatct
     1321 cagagccggc aggtggcctc gtactccatc agtttcgtga tttttggatg agggccgcag
     1381 agcggacagt taggatcttt gtgtatcttg acttcgcgga atctcatctc cagcgcatcg
     1441 taaagaacca ggcgcccggt cagagaactc ccgatgccga ggatctcttt taacgcctcg
     1501 gtggcctgaa gcactccgag tgtccccgcc agggctccca gcactccggc ctcctgacag
     1561 ctcgtcacga gatccgccgg aggtggttcc gggaagagac aacggtagca gggatggtct
     1621 gcgtatgcct tgatcgtggt gagttgacct tcgaatcgga aaatgctggc cgagatcagg
     1681 ggttttttca gaaaatagca ggcatcgttg accaagaacc tcgttggaaa attgtctgag
     1741 ccgtccagga taagatcgta atcggataga agctccatga tattggaggc ggaaagtttt
     1801 tcctggtagg tctcgatgtt gcagtccgga ttgatggcct ttagccgcgc ttttgcggac
     1861 tctaccttgg gttgatttat ggtcttcgtg gtgtgcagta cctgccgctg gagattcgag
     1921 agatccacaa cgtccgagtc aatcaaaccc agtttcccaa gaccacccgc cacgagatac
     1981 agagccgctg gagaaccgag gcctcccgcg ccgactaaga acagccgtgc gttccggagc
     2041 ttcttttggc ccttgcctcc gatctccggc aggatgatct gtcgagagta acgctggatt
     2101 tcttcatcgg tgaagttcat caggtgggtt attcaataat attgcgttcg atgggatcga
     2161 ccttgacccc tttgttcttc atgcccacga ccgcacgctc gatctctttg ggatcgccgg
     2221 tcagctcaag atctacccag ccggttttct ctgtgacatc cgcctttcgg atattcgtga
     2281 cgaccttata ttccttgccg atctggtaga tgatcggctc cttgatcttg ccttcgggaa
     2341 aggtaatatg gaattttaag cttgccactt gtcagcatcc tcctgcaatc gctggaacaa
     2401 tcgaaatctc atctccttct ttgagcgttg tgtcctcgcc ctttaaaaac cggatgtcct
     2461 cttcgttgac atagatattc acaaatcggc gcagatttcc ggtctcatca tagagacggg
     2521 tcttcatccc cgggaatttc ttctccagat tctcgatcag gtctttgata tttgacccaa
     2581 ctccatccac ttggccttga ccggcggtca gtggtcggag cggggagggg atgcgaacct
     2641 taaacgccat gagagctccc tttgccggac agggcggtgc cggtcttgtt gggtttaaag
     2701 gcttcttcaa aagcagcgag actgggctca agccgaacag gtgaacccac ggcatccatc
     2761 acagcttcct gggtcttcag gccgtttccg gtgatataag ccacaacaac ttctttcggt
     2821 ttgatgcggc caccttggac taattttttt aggacgccga ccgttacacc tcctgcggtc
     2881 tcggcaaaca ccccctcggt ctcggccaaa agctggatgc cttcgatgat ttcagcgtcc
     2941 gtcacggtct caaagacgcc cccgctctct tttgtggttt tcaaggcata gtagccatcg
     3001 gccgggtttc cgattgcgag tgatttagcg attgtcatgg gcttgaccgg tttgatgaag
     3061 tctcgctttt cacgaaaggc ggttgccacg ggagagcagc cttcggcctg tgctccattt
     3121 atgcgtgttt ttgcgctatc gatgatgcca agtgtctcaa actccttgag tcctttccag
     3181 atttttgtga gcaacgagcc ggaagcgacc ggtacaacga cctggtccgg tgcccgccat
     3241 ccgagttgtt ctgcggtttc atacgcgagg gtcttggagc cctccgcata gtacggccgt
     3301 atgttgatgt ttacaaaggc ccagttgtat tccgcggcga tctcgctgca gagccggttg
     3361 acatcgtcgt agttgccctc gaccgccacc acggtgggtt tataaatcaa attaccgagg
     3421 attttagccg attccagatc ggacgggatg aagacataac aattcagtcc ggcctgggcc
     3481 gcatgggctg cgaccgagtt ggcgagattg ccggttgagg cgcaggcaac ggtgtcgtag
     3541 ccgaactcct tcgctcgagt caaggctacc gctacgaccc ggtctttaaa agacagcgtg
     3601 ggatgattca ccgtgtcgtt tttgagatac aactcgttca gccccagcct ctttgccagg
     3661 cgcgtcgccc taatcattgg cgtcaggccg gcgtgaagac cgaccgtggc cggcccttcc
     3721 accggaagaa gatcaatata gcgccagaga ctattgggac cgcggctgat tgcttcccta
     3781 gagatctgct tcttgatctc gtcatagtta tactggacct ccagcggtcc gaagcaaaac
     3841 tcgcagacat gaagcgggtc ggtcggatac acctttccgc attcgcggca cttgaggcct
     3901 ttcatcttgt tgtttgtcat cttgtttatg ggttccctat atcctgcagg cctcttgttc
     3961 gtaatccatg agctcggtaa tctgtggatg ttcactacag accggacatt ttggattccg
     4021 tcgtagtttg acctcccgga agcgtgtgcc cagggcgtca taggtcagta gccgatccgt
     4081 caagaggttt ccgatcccca gtatgagctt taacgcctcg gtggcttgaa tggtcccgat
     4141 caatccggcc aaagcgccca gcacgccggc ctcctggcag cttgggataa ggccctgtgg
     4201 cggtgggttt ttgaagatgc agcggtagca ggtcgtctgc ttgggcagga tcgtcatcgc
     4261 ctggccgtcg
//
LOCUS       DATMKH010000447         1055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4682944, whole genome shotgun sequence.
ACCESSION   DATMKH010000447 DATMKH010000000
VERSION     DATMKH010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4682944"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..>1055)
                     /locus_tag="VNV63_05410"
     CDS             complement(110..>1055)
                     /locus_tag="VNV63_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62090.1"
                     /translation="TRSVNLPVLVSGAALLTTGLLGFSKEGIITPFICWIIAASSQRF
                     KVSRTQIVMGILGTIFIVRYLVPYSQYGRNYREETFQGNVRVSLTFLTDLSTVREEYL
                     AEQEETVERENLAYFDTPQGFFDRLQELSIDDALIHRTVENGPIGIYPIIFGFENLVP
                     HFIWKDKPTILYGNVYAHEIGLLAEEDTTTGVSFSPTSEAYHLAEWTGLLLLAPAIWI
                     VLFALFDSLCGDLTKSPWGLLVMLLYSHIAPEAGISGLIYSFGYTTIAIVIAAIFGAY
                     VMPVVGTFFIGPEGILIRRGAPIRSLPRRLLPPQPSEN"
BASE COUNT          278 a          254 c          308 g          215 t
ORIGIN      
        1 cgccgagttc tacggggaca aagtaagcca ttccgaacga cgaacgcttc aagaaaagcc
       61 atctggatcg caaaggcttc ccgcgcgtag cacaggattc tggaaaacac taattttcag
      121 atggctgagg tggaagaagg cgcctgggca aactccgtat aggtgctccg cggcggatga
      181 gtattccctc cggtccaatg aagaaggtcc cgaccaccgg catgacatag gcaccgaaga
      241 tcgccgcaat cacgatggca attgtggtgt agccgaagct gtagataagg ccgctgatac
      301 cggcttccgg ggcgatgtgg ctataaagaa gcatgaccag caatccccaa ggcgatttcg
      361 tgaggtctcc gcacaacgaa tcgaataatg cgaatagaac aatccatatc gcgggcgcaa
      421 gcaggagcag tccggtccac tcggcaagat ggtaagcctc cgaggtggga gaaaaggaga
      481 ctccagtcgt ggtgtcttcc tcggcgagga ggccgatctc gtgggcatag acatttccgt
      541 acaggatcgt gggcttgtct ttccagatga aatgcgggac caggttctca aatccaaaga
      601 taatcgggta gatcccgatg gggccattct ccacagtgcg atggatcagg gcgtcatcga
      661 tggagagttc ctgcagacgg tcgaagaagc cttggggagt gtcgaaataa gcgagatttt
      721 ctcgttcgac tgtttcttcc tgttcagcaa gatattcctc gcgaacagta ctcaggtcgg
      781 tgaggaaggt gagtgagact cgcacatttc cctggaaagt ttcctcccgg taatttcgcc
      841 catattgtga atagggaacc agataacgaa caatgaagat ggtcccgagg atgcccatta
      901 cgatctgagt ccggctaact ttaaatcgct gagaggaggc agcgatgatc cagcaaatga
      961 agggcgtaat gatcccttct ttggaaaaac caagaagacc cgtcgtaagc agtgcggcgc
     1021 cggagacgag aacggggaga ttgacgcttc gcgtt
//
LOCUS       DATMKH010000448         2016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4683479, whole genome shotgun sequence.
ACCESSION   DATMKH010000448 DATMKH010000000
VERSION     DATMKH010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4683479"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="VNV63_05415"
     CDS             complement(<1..475)
                     /locus_tag="VNV63_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018439245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HXC62091.1"
                     /translation="MTKTSLITTIAVVCSLTFLGTVGDSQDKPIKKDLKLAFVPKNIN
                     NPYNVIETKGSLDACQEISAQGKVVGPSDASASSQVSYINTLITQRQNAIVLAANDPN
                     ALIPYLKRAREQKIQIVTMDSDTAPEGRKVFINQASSEGIGQGQVQILAKQIDFKG"
     gene            complement(894..1754)
                     /locus_tag="VNV63_05420"
     CDS             complement(894..1754)
                     /locus_tag="VNV63_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62092.1"
                     /translation="MSANLNLLRQKYCRPASFAGKRTDHLFPLEEECEEGRALLATAP
                     KTLLHFQEFDPYGLQQTYQEEVSGAELPIFAVFDLEGSHQLTFEITTDSLLTIQQPAG
                     LAAYLPYQRTEAFVRKINDRRIKSERALSGLSMVLGIFPVLILLLSHTIVMVREAVPY
                     VFVGGWVLGAFFLYILSLFVLNQSFPWKKLAITAEFNGILPKEVREKARAAKDHFEKL
                     YLIVDQQHRWKSVLLPDPRPRVLDPLLIGELRQGRARRYYLIHQFDLTEAEQYLADEF
                     AVKPDDIPRS"
BASE COUNT          457 a          486 c          565 g          508 t
ORIGIN      
        1 cgcctttgaa atcgatctgt ttggccagga tctgtacttg cccttgaccg atgccttcag
       61 aggaagcctg gttgataaaa accttgcgac cttccggggc ggtgtcggag tccatcgtta
      121 cgatctgaat cttctgttcc ctcgcccgct taaggtaagg aatcagggcg ttcgggtcat
      181 tggcggcaag cacaatcgca ttttgccgct gggtgatcag cgtgttgatg taggatactt
      241 gggaggaggc ggacgcatcg gacggaccga ctacctttcc ttgcgcgcta atctcctgac
      301 aagcgtccag agagcctttc gtttcgatca cattgtaggg gttattgatg ttcttgggaa
      361 caaatgccag tttgagatcc tttttgatcg gcttgtcctg gctatcgcca actgttccaa
      421 gaaatgttag ggagcaaacg acagcgatgg tggttatcag ggaggttttg gtcataggat
      481 gttcatgggg ctatgctaag atctcccgac cgacttatct atacaatcgt gaaaagggaa
      541 atgggaaatg ggaaaagcgg attgcggaat tcggaatggg aagattgcga agctccaaaa
      601 attgttgaat tgggaatacg aaatttcccc aattcggcaa tccgcatttc ctccatttcc
      661 ccatccccaa ttccgcattc cgcaatccgc aattccttcg taggactcag cccggcgttg
      721 caacgccggc gacatccaac aaacgggtat ttctgcaagg gcgtaagatc ggcgatcgtt
      781 accgtcgagt atggtgatac ggggagattc ccggcgtcaa gacgccgggc tggagtttgg
      841 acatttcagc atttatcgcg gtggcggtgc cggcgtatcc cgcgttctaa atctcacgag
      901 cggggaatgt cgtcgggctt gaccgcaaat tcgtcggcga ggtactgctc cgcctcggtg
      961 agatcgaact ggtgaatcaa gtagtatctg cgtgcgcgac cctgccttag ctcgccgatg
     1021 agaagcgggt ctaagacacg cggccttgga tctggaagga gcacgctttt ccagcggtgc
     1081 tgctggtcta cgatgaggta gagtttttcg aagtgatctt tggcggcacg cgctttttcc
     1141 cggacttctt tcggcagaat tccgttaaat tctgcggtga tggcaagctt cttccacgga
     1201 aaagactggt tcagcacaaa caggctcaag atgtataaga agaatgcgcc aaggacccat
     1261 ccccccacaa agacataggg taccgcctcc cttaccatta cgatggtgtg ggacaggaga
     1321 agaattaaga cagggaatat tcccagaacc atggagagcc cggatagcgc tcgttcggat
     1381 tttattctgc gatcattgat tttacggaca aatgcttccg tcctttgata aggaaggtac
     1441 gcggcaaggc ccgcgggctg ttggatagtg aggagcgaat ccgtggtgat ttcgaaagtc
     1501 aattggtggc ttccttcgag atcgaaaaca gcgaagattg gtagttctgc accgctaact
     1561 tcctcttgat atgtctgttg aagaccgtac ggatcgaact cttgaaaatg aagaagtgtc
     1621 tttggtgctg tcgccagcag ggctcttccc tcttcgcact cttcctccaa gggaaagaga
     1681 tgatcggttc ttttgccagc gaagctggca ggccggcagt atttctgccg gagcaggttc
     1741 aggttcgcgc tcatcagggg ttcctggaaa gttggttttg ccgttccctt caggtccttg
     1801 ctcccaaggc ttctagaaag ggagattttt caccttctgg atcgtcgccg aagtctgcct
     1861 aaaacgcaaa tcgaaccgtg gaaagatgac acaggctgcc aacctccttc cacgggggca
     1921 agacaggcca attttctggc atttccaatg gcggatgacg gactatctgg aagcctgatg
     1981 aaagccttga cgctggcgtt gatattagtg cgttgg
//
LOCUS       DATMKH010000449         1092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2774208, whole genome shotgun sequence.
ACCESSION   DATMKH010000449 DATMKH010000000
VERSION     DATMKH010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1092
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2774208"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="VNV63_05425"
     CDS             <1..385
                     /locus_tag="VNV63_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HXC62093.1"
                     /translation="GAKQAPDLTPLSSELSSMRSRVAPFIKAGAVLVVGGEVGSTAGS
                     SEAAGRPALSLEGEIRSREDVLGLLQKICQFYERIEPSSPVPLVLKRAARLAEMDFMQ
                     IMQDLSPDAISQIRIITGEKEAEEG"
     gene            627..>1092
                     /gene="tssB"
                     /locus_tag="VNV63_05430"
     CDS             627..>1092
                     /gene="tssB"
                     /locus_tag="VNV63_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003195557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HXC62094.1"
                     /translation="MAKASSQKFIARNRAPRVQIEYDVEVYGSQKKIELPFIMGVMAD
                     LSGKPVDPLAPVADRKFLEIDIDNFDDRLKAAKPHVAFRVPNTLTGEGNLSVDLTFES
                     MDDFSPGAVARKVESLNKLLTARNQLSNLITYMDGKVGAEEAVKKLLADPALL"
BASE COUNT          277 a          265 c          298 g          252 t
ORIGIN      
        1 cggcgctaaa caagcaccag acctgacccc gctttcgagt gaactaagct cgatgcggag
       61 tcgggttgct ccttttataa aggctggggc tgtgttagtc gttgggggag aggtcggctc
      121 gacagccgga tcttccgaag ctgctggtcg accagcactt tccctggaag gagagatacg
      181 ctcgcgggaa gatgtgctcg gtctgctgca aaagatttgt caattttacg aacggatcga
      241 accgtccagt ccggtaccgt tggttttgaa gcgcgcggcc cgtctggcgg aaatggactt
      301 catgcaaatc atgcaagacc tctcgccgga cgcaatttct cagattcgaa ttatcaccgg
      361 agaaaaggaa gcggaagaag gatgatcgcg gttgagtgca cggcgtgaca atgcaattga
      421 caaattgaac cggttttggc gaaatgatcc ggtcccaatc caggaagacc gatgagagaa
      481 cgatagacag atgcctaaag cgtttccagg ctacttcaga ctttcaagag agggcgtatg
      541 cgcctcgaaa acaatcggag gtggcagggt tattcgacag gtctttcgtt aaatctcccc
      601 cctccacaac ctaatgattc aagtttatgg ccaaagctag cagccaaaaa tttattgctc
      661 ggaaccgtgc gccgcgggtt cagatcgaat acgacgtaga ggtttatgga tcccagaaga
      721 aaattgaact cccgtttatc atgggtgtta tggccgatct ttccggtaaa cccgtcgacc
      781 ccctcgcgcc tgtggccgat cgcaaatttc tggagattga tatcgataac tttgacgacc
      841 gattgaaagc ggctaagcct catgtggcct tcagagtacc gaacaccttg accggggagg
      901 ggaatctaag cgtcgatctg acttttgaaa gcatggatga cttctcccca ggcgcggttg
      961 cgcggaaagt tgaatctctc aacaagttgc tcactgctcg aaatcaactt tccaatctaa
     1021 tcacttatat ggatggcaaa gtgggtgcgg aagaagcggt gaagaagctt ctcgctgacc
     1081 cggccctgtt aa
//
LOCUS       DATMKH010000450         1786 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_526082,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000450 DATMKH010000000
VERSION     DATMKH010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526082"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="VNV63_05435"
     CDS             complement(<1..565)
                     /locus_tag="VNV63_05435"
                     /inference="COORDINATES: protein motif:HMM:NF013155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62095.1"
                     /translation="MPKKPEGVASIEKIELHQHVDGSIPVRLTWELMKKHGINPVKTI
                     DEMERRLVLQKEEEGTLLSYLDKFHYPQWITQFYENISQVVEAIVEEAWNNNVRMLEL
                     RYSPIIHTYAGLTFRQTISAVLSGINHAKKRFPMAAGLVVIAMRQQGPHIAKILARQA
                     ISEAQHLHERTGVVGFDLAGAERGNPPG"
     gene            complement(570..1289)
                     /locus_tag="VNV63_05440"
     CDS             complement(570..1289)
                     /locus_tag="VNV63_05440"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXC62096.1"
                     /translation="MSHLKDQVAIVTGGSSGIGLAISRAFVREGMRVTIAARDKRRLN
                     QAALQLKSRSAGNDTVLAVQTDVSRATEVRQMVRQTLDRFGRLDVLVNNAGIGRLAPL
                     DRLKEKEWDETLGINLKGVFLCTQAVLPGMKRRRGGYVVNISSVAGKEGFAGGGAYCA
                     SKFGVMALTETLIEEAKSYDIRATAICPGYVDTPMVAGVPVPAAEMIRPEDIAQTVLY
                     LLHLSKNVIVREIVMTRTGTE"
     gene            1410..>1786
                     /locus_tag="VNV63_05445"
     CDS             1410..>1786
                     /locus_tag="VNV63_05445"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HXC62097.1"
                     /translation="MRRYAFLHFLALITLLDLSASCERKNPNADRMKDLFLSPSEYLV
                     GANPTSVTAADFNRDGNLDLITTNVGDDSLSFLQGNGDGTFQNEVKIQVGNQPRTLAV
                     GDFNEDGKLDLALSLIGNDSEVV"
BASE COUNT          385 a          536 c          456 g          409 t
ORIGIN      
        1 gacccggagg gttaccgcgt tcggctccgg ccagatcaaa tccgaccaca ccggttcgtt
       61 catggagatg ctgggcctcc gagatcgcct ggcgcgccaa tatttttgca atatgcgggc
      121 cttgctgccg catcgcgatc accaccaggc cggctgccat cggaaaccgt ttctttgcat
      181 ggttgatccc ggacagcacc gcgctgatcg tctgccggaa ggtcagcccg gcataggtat
      241 ggatgatggg ggaataacgg agctccaaca tccggacatt attgttccag gcttcctcga
      301 caatcgcctc caccacttga gagatgttct catagaactg ggtgatccat tgagggtaat
      361 ggaatttatc cagatacgag agcagcgtcc cctcctcttc cttctgaagg accaggcggc
      421 gctccatctc atctatcgtc ttcaccgggt tgatgccgtg cttcttcatg agttcccagg
      481 tgagacgcac cgggatcgag ccgtccacat gctgatggag ttcgatcttc tcgatacttg
      541 caacgccctc cggtttttta ggcatcgctc tactctgttc ccgtacgggt catcacgatc
      601 tcccggacta tcacattctt cgagagatgg agaaggtaca gaacggtctg ggcgatatcc
      661 tccggacgga tcatctcagc cgcgggaacc ggcacacccg ccaccatcgg agtatccaca
      721 tagccggggc agatcgcggt tgcccggatg tcgtaagact tggcttcctc gattagcgtc
      781 tcggtcaaag ccatcacgcc gaacttggaa gcacagtagg ccccgccccc cgcgaaccct
      841 tccttgccag cgacagatga gatattcacg acatagccgc cccggcggcg cttcatcccc
      901 ggaagaaccg cttgagtgca gaggaagacc cctttgagat tgatgcccag agtctcatcc
      961 cactccttct ctttgagtct atccaaaggc gccaggcggc cgatgccggc gttgttgacc
     1021 aagacatcca gccggccaaa acggtccagc gtttgtcgaa ccatctgccg gacttcggtc
     1081 gcccgagaga catctgtttg aacggcaaga acggtgtcgt tccccgcgga acgggattta
     1141 agctgtaaag cggcctgatt cagccggcgt ttgtcccgcg ccgcgattgt cactcgcatc
     1201 ccttcccgca cgaaggcccg ggagatcgca agaccgatcc cgctgcttcc tcccgtcaca
     1261 atcgcaacct gatctttgag atgactcatg ctttgcatca tatcacgaaa attcttcaag
     1321 ccgcaacacg atacggggta atccgcttgc atcctgatcg tgtgtatgtt accctctcga
     1381 aatattgaat ctccgataaa ggactctcga tgcgccgata tgcctttctt cattttcttg
     1441 ccttgattac cttgctcgac ctatccgcat catgtgaacg aaaaaatccg aacgccgacc
     1501 ggatgaagga cctgttcctt tccccgtccg aatatctggt cggggccaat cccacctcgg
     1561 tcaccgccgc ggattttaac cgtgatggaa atctcgatct catcaccaca aacgtgggag
     1621 acgattcgct ttccttcctt cagggaaatg gggacggaac tttccagaat gaagtgaaga
     1681 tccaggtagg aaaccagccc aggaccttgg cggtgggtga ttttaatgaa gacgggaaac
     1741 ttgatcttgc attgagcctc atcggcaacg actctgaagt cgtgat
//
LOCUS       DATMKH010000451         1344 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1804652, whole genome shotgun sequence.
ACCESSION   DATMKH010000451 DATMKH010000000
VERSION     DATMKH010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1344
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1804652"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="VNV63_05450"
     CDS             complement(<1..637)
                     /locus_tag="VNV63_05450"
                     /inference="COORDINATES: protein motif:HMM:NF045451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HXC62098.1"
                     /translation="MKKNIRTRVVLLVLSVLVSILFFLPSTPWYSSFPPWWKKYLPSK
                     GITLGLDLQGGIHLILEVEEDKAVENLTERTANYMKALFEEKKLSVQSVKRSSATEIV
                     IQYPESTKKEDLLKQIEPNFPTFGLKEDKPGELVFSLGEIEIKRIKDNATTQALETIR
                     NRIDQFGVAEPLIQRQGTNQIVIQLPGVKDPQRAKRLIGETAQLEFKLLNED"
     gene            complement(634..1020)
                     /gene="yajC"
                     /locus_tag="VNV63_05455"
     CDS             complement(634..1020)
                     /gene="yajC"
                     /locus_tag="VNV63_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013164116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HXC62099.1"
                     /translation="MKWEWLSGMVQTGGEAWAQAPGGLIGGNGMGTIGAVLPFVLIFV
                     LFYFLLLRPQQQRQSRQKQMLAALKKGDHVVTTGGMIGTVVALAKEVVTLQVAEKVRI
                     KVLRDSITEVRGEDLEEASSERGGAA"
     gene            complement(1017..>1344)
                     /locus_tag="VNV63_05460"
     CDS             complement(1017..>1344)
                     /locus_tag="VNV63_05460"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017866364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA-guanine transglycosylase"
                     /protein_id="HXC62100.1"
                     /translation="GCLFTTSGRLIIKNAQYTKDDQPIDPACPCYTCRHFSRAYLRHL
                     FMAKEILALRLNTIHNLYYYLSFMRELREAIRQNRLLKFREEFYRQRYHLGEARTGRA
                     EAENDQ"
BASE COUNT          293 a          385 c          318 g          348 t
ORIGIN      
        1 tatcctcatt caacagttta aattctaact gggcggtctc cccaatcaat ctcttggccc
       61 gttgcggatc cttcactccc ggcaactgga tcacgatctg attcgtgcct tgccgctgga
      121 tcagcggctc cgccacgccg aactgatcaa tacggtttcg gatggtctcc agagcctgag
      181 tggttgcatt gtcttttatg cgtttgatct ctatctctcc caatgaaaac acgagctctc
      241 cgggtttatc ctctttaaga ccgaatgtcg gaaagttggg ttcgatctgt tttaagaggt
      301 cttccttctt cgtcgattcc ggatactgga tcacaatctc ggtcgcactc gatcgtttga
      361 cagactgcac ggacagcttc ttctcctcaa agagggcctt catgtagttg gcggttcgtt
      421 ccgtcaggtt ctccaccgcc ttgtcttctt caacttccag tatgagatgg attcctccct
      481 ggaggtccag gcccagcgta atccctttgc tcggcaggta ctttttccac cagggcggaa
      541 aggatgaata ccagggcgtt gaaggtaaaa agaagaggat cgaaactaga acagataata
      601 ctaaaaggac tacccgcgtc cggatatttt ttttcatgcc gctccacccc tctcagacga
      661 agcttcttca agatcctcgc cgcggacctc cgtgatagag tcccgcaaga ccttgatccg
      721 caccttctcc gccacctgca gcgtcaccac ttctttggca agggccacaa ccgttccgat
      781 cataccgccg gtggtcacca cgtggtctcc cttttttaat gccgcgagca tttgcttttg
      841 gcggctctgg cgctgctgct gaggccgcag gagaagaaaa taaaagagca cgaagatcag
      901 gacaaacgga agcactgcac cgattgtacc catcccgttt ccaccgatta aaccgcccgg
      961 tgcctgtgcc caggcctcgc ctccggtctg caccatcccg ctaagccatt cccatttcat
     1021 tgatcgttct ccgcttctgc ccttccggtt cgggcctctc ccagatgata acgctgacgg
     1081 taaaactctt ctcgaaactt taagagccga ttctgtcgga tcgcttctct taattcccgc
     1141 atgaaggaca ggtaataata aaggttatgg atcgtattga gccgtagcgc caagatttct
     1201 ttcgccataa agagatgccg cagataggcc cgtgagaaat gccggcaggt gtagcaaggg
     1261 caggccggat caatcggctg atcatctttt gtgtactggg cattcttgat aatgagccgg
     1321 ccgctggttg taaacaaaca gccg
//
LOCUS       DATMKH010000452         2662 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2776260, whole genome shotgun sequence.
ACCESSION   DATMKH010000452 DATMKH010000000
VERSION     DATMKH010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2776260"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /locus_tag="VNV63_05465"
     CDS             <1..520
                     /locus_tag="VNV63_05465"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXC62101.1"
                     /translation="LKGIKELLKAAIEEARNVIFNLRPLSFDKGGLIQALKNYLATYE
                     RQYHIKTDLKVSVEEKKILPKFKVFLFRIIQETLSNVHRHAKAKKVWISLDISHALFK
                     TTIQDDGIGFDLRQVARDPEKWASFGLKGIKERCRLLSGTAAIESRPGHGTRITIELP
                     LEEKGPKRREKD"
     gene            507..1205
                     /locus_tag="VNV63_05470"
     CDS             507..1205
                     /locus_tag="VNV63_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXC62102.1"
                     /translation="MKKIKVLIADDHQVVREGLSAILRTKEEIEVVGEVKDGAEAVEQ
                     ARRLKPDVILMDISMPGMNGVEATRQIKREQPEIGVVVLTMYEEEDYIYDLVKAGATG
                     YLLKDSDSSQIVKAIKTVARGESLIHPSVASKILHEFSSLAQGKGQPVKKKTQELSDR
                     EIEVLKLVAEGKTNKEIANVLNLSEKTIKNHVRNIFHKLQVYDRTQAAMHGLRKGLIQ
                     LDGPPRPDSKQGKS"
     gene            1210..2106
                     /locus_tag="VNV63_05475"
     CDS             1210..2106
                     /locus_tag="VNV63_05475"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXC62103.1"
                     /translation="MTEALMPQTLIPPSSLDENSLERLIVQMSGLPRDSGTLNLDLGR
                     VEFIDPCGMVGLLELGRYFAQQGHKLLLHLPLSMEVQRCLDQMEFFRHLSEFYIMYPP
                     YRPLAARARQVQQSDVLLEITRIAQSDDIPRIIGKVKERTRAILEAHLHYKEDAIQGF
                     LVALHEICQNILEHSQNTGLAGIQKYFDEKHLNKNAVKVAVMDLGIGFKGSLSSRPGL
                     RHGEQWSDIAALEEALLNGAGRGHGLATVKEFVQRWGGKLSIRSGTARLLFLSDREHG
                     SERETGLSEFPGVLISLVLSEV"
     gene            2119..>2662
                     /locus_tag="VNV63_05480"
     CDS             2119..>2662
                     /locus_tag="VNV63_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62104.1"
                     /translation="MTISLWQKFRDTTNHAAQWLIQESSDTLHSATRWLEGRSFCCLK
                     CHTTMRGSTTHCPTCGARFVPEAIEAQAKSRLTAILSDLSKQKPIRYLTDFLKPDHPV
                     THRILKSFVVIAVESEGYSLFMLFNVTIFSLVTPGTAAVIPFLAYLLYHYRGLFAERS
                     LKPLRSHIQDLKESLKQNRIT"
BASE COUNT          659 a          730 c          715 g          558 t
ORIGIN      
        1 gctgaaaggg atcaaagagc tgctcaaggc cgcgattgaa gaagcacgga atgtgatctt
       61 caacctccgg cctctttcgt ttgataaagg ggggctgatt caagctctca agaattacct
      121 tgcaacctac gagcgccagt atcatatcaa gacggatctc aaggtatcgg tcgaagagaa
      181 aaagatcctg cccaagttca aggtattcct cttccggatc atccaggaaa ccctttccaa
      241 tgtccaccgc cacgccaagg ccaagaaggt ttggatatcg ctcgatataa gccatgcact
      301 gtttaagaca acgatccagg atgacggcat cggctttgat ctccgccagg tcgcccgaga
      361 ccccgagaag tgggcctcgt tcggattgaa agggatcaaa gaacgatgcc ggcttctgag
      421 cggcacggcc gcgattgagt cacgtcccgg tcacggcacc cggatcacaa tcgaacttcc
      481 cctggaggag aagggaccca aacgccgtga aaaagattaa ggtcttgatt gcggatgatc
      541 accaggtcgt ccgggaaggg ttaagcgcaa tccttcgaac taaagaagag atcgaagtag
      601 tgggagaggt aaaggacggc gcagaggcgg tggagcaggc ccggaggctc aaaccggacg
      661 tgatcctgat ggacatcagc atgccgggga tgaatggtgt cgaagccaca cgccagatca
      721 aacgagagca accggagatc ggggtagtgg ttctcaccat gtatgaagag gaggattaca
      781 tctatgacct ggtcaaggcc ggggcgaccg gttacctcct caaagactcg gactcctctc
      841 agatcgttaa agcgattaaa accgtggctc gcggcgagtc tctaatccac ccctcagtcg
      901 caagcaaaat cctccacgaa ttctcttccc tggcccaagg caaaggccag cccgtaaaga
      961 agaagacaca agagctctcg gatcgcgaga tcgaggtatt aaagctcgtg gcggagggaa
     1021 agaccaacaa agagatcgcc aacgtcttaa acttgagcga gaagacgatc aagaaccatg
     1081 tccgcaacat tttccacaag ctccaggtat atgaccgcac acaggcggca atgcatggtc
     1141 tgcgcaaagg tttgatccag ctcgacggcc cgccccggcc ggattccaag cagggcaagt
     1201 cctgaaactg tgactgaggc actcatgccg cagacattga tcccgccctc ctcgctggat
     1261 gagaacagcc ttgagcgatt gatcgttcaa atgagcggtc tcccgcgtga ttcaggcaca
     1321 ctgaatctag accttggccg agtcgagttt atagatccct gcggcatggt gggactcttg
     1381 gagcttggcc gttacttcgc gcagcaaggt cacaaactcc tgctacatct tcctctctca
     1441 atggaagtcc agcgctgcct ggaccagatg gagttcttcc ggcatctttc cgaattttat
     1501 atcatgtatc ctccttacag gccgcttgcc gctcgcgccc ggcaggttca acaatctgac
     1561 gtcttgcttg agatcacgcg gatcgcgcaa tcggatgaca tccccaggat tatagggaag
     1621 gtcaaggagc ggacccgggc catcttggag gcccatctcc attataaaga agatgccatt
     1681 cagggtttcc tggtggcgct ccatgagata tgccaaaata ttcttgagca cagccagaac
     1741 accgggctcg cggggatcca gaaatatttt gatgagaagc atctaaacaa aaatgcggtc
     1801 aaggttgcgg tgatggatct gggcatcggc tttaaaggat ctctttcatc tcgacccggc
     1861 ttgcggcatg gtgagcagtg gtcagacatc gcggcccttg aagaggcact gcttaatgga
     1921 gccggacggg gtcatgggct tgccacggtc aaagaatttg ttcaacgatg gggtggtaaa
     1981 ctttcgatcc gatccggcac ggcaaggctg ttgtttctat cggatcgaga gcacggcagt
     2041 gaacgtgaaa ccggactatc ggaattcccg ggtgtcctga tcagcttggt cttgtccgag
     2101 gtgtagtata gagacgtaat gacgatctct ctctggcaaa aattccggga caccaccaac
     2161 catgccgctc agtggctgat acaagaaagc tccgacactc tgcactcagc tacacgctgg
     2221 ctggaaggac gctccttctg ttgtctcaaa tgccacacca cgatgcgcgg ttcgaccacg
     2281 cattgtccca cttgcggggc gcggttcgtg ccggaggcga tcgaggccca ggcgaaaagc
     2341 cgattgaccg ccatcttgag cgatctctcc aagcagaagc cgatccgcta tcttaccgac
     2401 tttctgaaac ctgatcatcc cgtgacccac cgtattttga aatcttttgt ggtcatcgcg
     2461 gtggaatccg aggggtacag cctcttcatg ctgtttaacg tcaccatctt cagcctcgtc
     2521 acaccgggca ccgccgcggt catcccgttc ctggcttatc tcctctacca ctaccggggc
     2581 ttgtttgcgg aacggtcctt aaaaccgctc cgctctcaca tccaagacct caaggaatcg
     2641 ctaaagcaga accggatcac ga
//
LOCUS       DATMKH010000453         1194 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2445986, whole genome shotgun sequence.
ACCESSION   DATMKH010000453 DATMKH010000000
VERSION     DATMKH010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2445986"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..>1194
                     /gene="dxs"
                     /locus_tag="VNV63_05485"
     CDS             113..>1194
                     /gene="dxs"
                     /locus_tag="VNV63_05485"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HXC62105.1"
                     /translation="MGLLEQIDSPADLKKLKPEDFPALAAEIRELIIQVVSKTGGHLS
                     SNLGVVELTMVLHYLFDTPKDQIVWDTSNQTYTHKLLTGRQKDFPTLRQYDGLSGFAK
                     REESIYDVFNAGHAGTGLSAAVGLVEARDLRGEDYKVICVVGDGALTAGMSYEGLNHA
                     GASKKDLLVILNDNEMSISKNVGALSAYLNRIITGQFVTRLKAETKSILRSIPRIGES
                     VIKAVEKVEESAKGLIGPGILFEELGFQYVGPIDGHNLTHLLETLENVKKLKGPILMH
                     VITKKGLGYEPAERDSVFFHSAPSFIRETGEPRKRSSIPSYTTIFSNTLIKLAKQDKR
                     IVGITAAMCDGTGLGKFAKEFPDRYY"
BASE COUNT          299 a          295 c          332 g          268 t
ORIGIN      
        1 agtttttctt gacatgccgg gggtttttgg atagaatcaa gtccacgtat ttttagaaca
       61 attcagttaa gtaacgaatt cagttggttc aacccacctt ggaggggaaa gaatgggttt
      121 attagagcaa attgatagcc ctgcagattt aaaaaaactt aaaccggaag acttcccggc
      181 gcttgcagcg gagatccgcg agttgatcat tcaggtggtt tccaagacag gcggccacct
      241 gtcatccaac ctgggtgtgg tggagcttac gatggtcctg cattatctct tcgatacacc
      301 gaaggaccag atcgtttggg acacaagcaa tcagacctac acgcataaac tcctcacggg
      361 ccgtcaaaag gatttcccga ctctccgtca atacgacggg ttgagtggat tcgcgaagcg
      421 ggaagagagc atctatgacg tgtttaatgc cggccatgcc ggaacggggc tttcggctgc
      481 ggtcggactt gtcgaggcgc gggaccttcg cggcgaggat tataaagtca tctgtgttgt
      541 cggagacggg gcgctgaccg cgggcatgtc ctacgagggc ttgaatcacg cgggcgcatc
      601 gaaaaaagac cttctcgtca tactcaacga caacgagatg tccatctcta agaatgtcgg
      661 agccctctcg gcctatttga accggattat cacgggacag tttgtcacgc gcctgaaggc
      721 cgagacgaag tccattctcc ggtccattcc aaggatcggc gaatcggtga tcaaagcggt
      781 ggagaaggta gaggagtcgg ccaaggggct tatcggaccc ggtattctgt ttgaagagct
      841 gggctttcag tatgtgggac cgattgacgg ccataacctc actcaccttc tcgagacgtt
      901 ggagaacgtc aaaaaactca aagggcccat actgatgcat gtcatcacaa agaaaggcct
      961 gggctatgag ccggcggaga gagactcggt cttcttccac agcgctcctt cctttattcg
     1021 ggaaaccgga gagcctcgga agagatcgtc gatcccgtct tataccacga tcttctccaa
     1081 caccttaatc aagctcgcca agcaagacaa gcggatcgtg ggaatcacgg ccgcgatgtg
     1141 cgatgggacc gggcttggaa aattcgccaa ggaatttccg gatcgctact atga
//
LOCUS       DATMKH010000454         1631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_852968,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000454 DATMKH010000000
VERSION     DATMKH010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_852968"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..1559
                     /gene="trpE"
                     /locus_tag="VNV63_05490"
     CDS             75..1559
                     /gene="trpE"
                     /locus_tag="VNV63_05490"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HXC62106.1"
                     /translation="MYYPTFESFCKKARQGNLIPVYREILADLETPVSAFLKVNQGRG
                     AFLLESVEGGEKWARYSFLGIDPAIIIKSRGDSVEVIRNGRVERLSFATDPLEVVKKI
                     MAQYRPVEVEGLPRFFGGAVGYLGYDLVRYLEKLPPRDKKGLDLPDLALMITDSLLIF
                     DNITQKIKVVTNVHLTHADLKKAYGAAVRKIDGIVKRLQGPRKIARRNRPVRRFKGLD
                     LSSNTSPSNFKKTVMKAKDYIRAGDIIQVVLSQRFETKIRADSFTIYRALRLVNPSPY
                     MYYLQLGDLDLVGSSPEVLVRCDEGKIEVRPIAGTRPRGKTEEEDKKLTGELLSDAKE
                     RAEHLMLVDLGRNDVGRIAEVASVKVDEFMAIEKYSHVMHLVSHVQGRLQPGKDGYDV
                     IRACFPAGTVSGAPKVRAMEIIEELEPTRRGPYAGAVGYFSFSGNLDTCINIRTILIK
                     DGIAYLQTGAGIVADSDPAREYEETVNKAKAMVAAIELAERGLE"
     gene            1560..>1631
                     /locus_tag="VNV63_05495"
     CDS             1560..>1631
                     /locus_tag="VNV63_05495"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018048495.1"
                     /note="TrpG; with TrpE catalyzes the formation of
                     anthranilate and glutamate from chorismate and glutamine;
                     TrpG provides the glutamine amidotransferase activity;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate/aminodeoxychorismate synthase
                     component II"
                     /protein_id="HXC62107.1"
                     /translation="MLLMIDNYDSFTYNLVQYLGELGA"
BASE COUNT          389 a          395 c          471 g          376 t
ORIGIN      
        1 ccctctttcg cttaaattga aattttcaag caattatgct accgttataa ctcttctaat
       61 gacaagaggt ttacatgtat tacccgacgt ttgaaagttt ctgtaaaaaa gcaaggcagg
      121 gcaacctgat cccggtttac cgagaaatcc tggcggacct tgagacaccg gtgtccgctt
      181 ttttaaaagt caatcagggt cgcggagcct tcctgctcga aagtgtggag ggcggtgaga
      241 aatgggcccg gtattctttt ctgggcatcg atccggccat cattatcaag agtaggggag
      301 atagcgtcga ggtcatccgt aacggtcggg tggaacggct ttcttttgca accgatccgc
      361 tcgaagtggt aaaaaagatc atggcccagt accggccagt cgaggttgag gggttgccgc
      421 gctttttcgg cggtgcggtg ggatacctgg gctacgatct tgtccggtat ctggagaagc
      481 ttccaccgcg cgataaaaag ggtcttgatc ttccggatct cgcactgatg atcacggata
      541 gtctattaat ctttgataat atcacgcaga agatcaaggt cgtgacaaat gttcatctga
      601 cgcatgccga tcttaaaaaa gcatacgggg cggccgtacg aaaaatagat gggatcgtca
      661 aaagacttca agggcctaga aagatagcgc gtcgaaaccg accggtgcgt cgtttcaagg
      721 ggcttgacct ctcttcgaac accagtccct ctaattttaa aaaaactgtg atgaaggcta
      781 aggactatat ccgggcggga gacatcattc aggtcgtcct gtcacaacgc tttgagacta
      841 agatccgggc ggattccttt acgatctatc gtgcgttgcg gctggttaat ccatcgccct
      901 atatgtacta cctgcaactc ggggatctgg atctcgtggg ctcctcaccg gaggtgctgg
      961 tccgttgcga tgagggaaag atcgaggtcc ggccgattgc gggtactcgg ccaagaggca
     1021 aaaccgaaga ggaagacaag aagctgaccg gtgagctgct ctcggacgcc aaggagcggg
     1081 cggagcatct catgctggtg gacctaggcc gcaatgatgt ggggcgcatt gccgaagtcg
     1141 catcggtgaa ggtggatgaa ttcatggcga tcgaaaaata ctcccatgtc atgcaccttg
     1201 tgtcgcatgt gcaaggcagg cttcagcccg ggaaggacgg ctacgacgtc attcgagcct
     1261 gttttcctgc cggcaccgtc tcgggggcac cgaaggtccg ggcgatggaa atcattgaag
     1321 agctggagcc cactcgtcgg ggaccctacg ccggggcggt cgggtacttc agcttctccg
     1381 gcaatctcga tacctgcatc aatatccgga cgattctcat caaagacggc attgcctatc
     1441 ttcagaccgg cgccgggatt gtggcggatt cggatccggc ccgtgaatat gaagagaccg
     1501 tgaacaaggc gaaagcaatg gtcgcggcga tcgagctcgc tgagagggga ttggaataga
     1561 tgctcctcat gatcgataac tatgactctt ttacttacaa cctggttcag tatctgggcg
     1621 aactgggagc c
//
LOCUS       DATMKH010000455         2258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4383758, whole genome shotgun sequence.
ACCESSION   DATMKH010000455 DATMKH010000000
VERSION     DATMKH010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4383758"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            20..1531
                     /locus_tag="VNV63_05500"
     CDS             20..1531
                     /locus_tag="VNV63_05500"
                     /inference="COORDINATES: protein motif:HMM:NF024927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HXC62108.1"
                     /translation="MPGIVGLITKMPSDRATAVLHRMLETVRHEPFYSIGTWIDESLG
                     VYVGWTALKDSFSDGMPSCNERSDVSLVFSGEEYPEPGTARRLKELGHSLGSEISSYL
                     VHLYEENPKFLVGLNGMFHGVLMDRTAGTATLFNDRYGMHRIYFHESKEAFYFAAEAK
                     AILEVCPELRVADLRGLGEFVSFSCVLENRTIFKSIQVLPSASAWVFRNGSIELRDTY
                     FHPKEWEEQTPLEPEPYYRELLDVLSRNLPRYFNGHERVGMTLTGGLDTRVVMALRKP
                     NPGSLPCYTFGGMFRECQDVQIARKVAKICQQSHEVITVGGDFLTRFPQYAERCVHLT
                     EGGIDVSRCSDLYVSEKARNIAPVKVVGTYGSEILRQVVLFKPVDPPPGLYCPDFLAN
                     VNQAGETYAEVRRTHPVTFAAFRQSPWYHRGVLSLEESQLTVRSPFLDNNFVQTVYRA
                     PKSADLKEDVRLRLIHDGNPTLGQIRTDGGIGGNSGRVTSRLTQFSLNFLSKA"
     gene            1550..1843
                     /locus_tag="VNV63_05505"
     CDS             1550..1843
                     /locus_tag="VNV63_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62109.1"
                     /translation="MPQWVARIDHHLSALHLERIFLGRNKLLHFRLWYRDSLSQYIQQ
                     ILLDPVSLSRPYIEKKGLEAVVRSHTKGDRNYTQEIHKLLSLELLHRLFLDPR"
     gene            1840..>2258
                     /locus_tag="VNV63_05510"
     CDS             1840..>2258
                     /locus_tag="VNV63_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62110.1"
                     /translation="MIQDVGTIAFIIGIFVLFAFDYDREARTLKALWLPVIWLFLAGS
                     RPVSLWFHIRVIGTSTDDLLEGSPLDRAVITVIIVLALIVLFARGRKVLPLLRANAPI
                     LLFLGYCTASILWSDFPDVAFKRLIRAIGDFSMILVIL"
BASE COUNT          510 a          593 c          594 g          561 t
ORIGIN      
        1 ttagaaggag gataggcctt tgccgggaat cgttggtctc atcacaaaaa tgcccagcga
       61 tcgggccact gcagtactac accggatgct cgaaactgtc cgtcatgagc ctttctactc
      121 gatagggact tggattgatg aatccttggg ggtgtacgta ggttggactg cgttgaaaga
      181 ttccttctcc gacggaatgc catcttgtaa cgaaagaagc gatgtctctc tcgtcttttc
      241 aggcgaagaa taccctgagc caggaactgc tcgccgcctc aaggagctgg ggcattcgct
      301 cgggtcggaa atttcctcat atctggtaca tctctacgag gaaaatccga aattcctggt
      361 gggtctgaat ggaatgtttc acggcgtcct gatggaccgg acagctggaa cggcgacgct
      421 attcaacgac cggtacggga tgcaccggat ctatttccat gagtctaaag aggcgttcta
      481 tttcgccgcg gaagccaaag ccattttgga ggtatgcccg gaactccgag tggcagacct
      541 cagaggctta ggcgagttcg tttcgtttag ctgcgttctt gaaaaccgga cgatcttcaa
      601 gagcatccaa gtccttccat ctgcttcagc gtgggtcttc cgaaacggct caattgagct
      661 aagagatacg tacttccacc caaaggaatg ggaggagcag acccctctgg aaccggaacc
      721 gtactaccgg gaactcctgg atgttctctc gcgaaacctc ccgcggtact tcaacggcca
      781 tgaacgggtg gggatgactc taaccggggg cttggatacg cgtgtggtca tggcattgcg
      841 caagccgaac ccaggatcgc tcccctgtta tacctttgga ggaatgttcc gagaatgcca
      901 agacgtacaa atcgcgagaa aagttgccaa aatatgccag caatctcatg aagtaatcac
      961 ggttggaggc gatttcctta cacgcttccc ccagtacgcc gagcgctgcg tacatctcac
     1021 ggagggaggg attgacgtat ctcgttgttc tgatctttac gttagcgaga aagcccgcaa
     1081 catagcgccg gtgaaggtag ttgggactta cgggagtgag atcctccgtc aagtcgtact
     1141 attcaaaccg gtggatccgc caccgggatt gtattgccct gattttttag caaacgtgaa
     1201 tcaagctgga gaaacttatg ccgaggtgcg gcgtacgcac cctgtcacgt tcgctgcttt
     1261 ccggcagtct ccttggtacc atcgtggagt cctatcgctc gaggagagtc agctcacagt
     1321 gcgatctcca ttccttgaca acaatttcgt ccagacggtt tatcgggcgc cgaagtccgc
     1381 tgacttgaag gaggacgtcc gtctgaggct catacatgac ggcaatccga ctcttggtca
     1441 gattcgaacc gacgggggca tcggagggaa ctcgggtcgc gtgacctcac gcttgaccca
     1501 attttccctt aatttcctga gcaaagccta gtatgcttac gactacggaa tgccgcagtg
     1561 ggttgctcgt attgatcatc acctttcggc gctccacctc gaacgtatct ttctcggtag
     1621 aaataagttg ttacatttcc ggctttggta ccgggattca ctttcccagt atatccaaca
     1681 aatattgctc gatccagtgt cactttcccg gccctacatt gagaagaaag gtctcgaagc
     1741 cgtcgtgcga agccatacca aaggcgacag gaactatacc caggagatcc acaagttgct
     1801 ctcgttagaa ctactccacc gtctcttcct ggacccaaga tgattcaaga cgtcggcact
     1861 atcgccttca tcatcgggat cttcgtcctg ttcgcctttg attatgatcg agaggcgaga
     1921 acattgaagg ccctctggct tcccgtgatc tggttgttcc tggcaggatc gcgaccggta
     1981 tcgctgtggt ttcacataag ggtcataggc acgtcaaccg acgatctcct cgaggggagt
     2041 cccctcgacc gcgcggtcat caccgtgatc atcgtactag cgttgatcgt cttattcgca
     2101 agaggaagga aggttttgcc actgctgcgg gcaaacgcac ctatcttgtt attcctgggc
     2161 tattgcacag ccagcatcct ctggtcagat tttcctgatg tggcgttcaa gcggttgatt
     2221 agggctatag gcgacttctc aatgatttta gttatcct
//
LOCUS       DATMKH010000456         2555 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3410451, whole genome shotgun sequence.
ACCESSION   DATMKH010000456 DATMKH010000000
VERSION     DATMKH010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3410451"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..1226)
                     /locus_tag="VNV63_05515"
     CDS             complement(48..1226)
                     /locus_tag="VNV63_05515"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HXC62111.1"
                     /translation="MTLQTESFVQTYDVLIVGLGPAGSTAAYELSKAGYSVLAFEKKS
                     MPRYKPCGGGLSQRIEKILEPDFKEVVEQTIYGVHFTFKGNDDLHVESSKPIAYMVMR
                     DRFDALLASKAKRAGTHIVEGETVLEIMDHSQGVEIRTAQRRYRGRYLIGADGANSMV
                     ARRLFPDRRVYKTISLESELPLGRMPDLDLRRKVLVDFERIPGGYGWIFPKQDKLSIG
                     IAGFRMKRWDPHHYFHRFTKGQGSLTGLPIPKPMGHPIPLFQGPPPVLSRQRILLAGD
                     AASLVDPFFGEGIYYAVRSGQLAAITLTNIFKNGHADLSEYDRMVSAEFYPEFRIASR
                     LAFIVYAFPRLWYWVNKRHHEFIELYYDVLRGVMSYEGFVQEIKKALKGMISLSWRLP
                     "
     gene            complement(1263..2363)
                     /locus_tag="VNV63_05520"
     CDS             complement(1263..2363)
                     /locus_tag="VNV63_05520"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HXC62112.1"
                     /translation="MWDNKFLDRHRLFALLLIGTLIQISLKPSRAEAEAQDNEPRAFT
                     FAELVKKEKPAVVNISIIQEVQGAGTLPGRSHPFREFFGDVLPREFKDTSLGSGFIIN
                     PNGLILTNQHVIEKAEKILVTLSDKRQFEAKLIGVDPKTDIALIRIKNQEPLPFVKLG
                     DSDRAEVGEWVLAIGNPFGLEQTVTVGIVSAKGRAIGSGPYDDYIQTDASINPGNSGG
                     PLFDIRGEVIGINTAINPSGQGIGFAIPINQVKTVIGQLEKEGKVTRGWLGVMIQEFT
                     KDLAKSFGIKKGEGALVSDVFEDSPAALAGIKKGDVIVEFNGKKIGHMRDLPVVVAGT
                     PIGKQASLKILRDGKEQNVIVIISRMEETKDR"
     gene            complement(2350..>2555)
                     /locus_tag="VNV63_05525"
     CDS             complement(2350..>2555)
                     /locus_tag="VNV63_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HXC62113.1"
                     /translation="LGLPLFLTSLGVNSFLTYFKQVRHHLRMITTVSGLFLIVVGVMI
                     YTNSLTLITAFLSQHGIGWYVGQ"
BASE COUNT          591 a          700 c          628 g          636 t
ORIGIN      
        1 tcgtaacgtt cgctcaagag acattttcag tgtacgctag gtaggtctca aggtaagcgc
       61 cagcttaagg agatcatccc cttaagcgcc ttcttgatct cttgaacaaa cccctcatag
      121 ctcatcactc ctcgaagcac atcatagtaa agctcgatga attcgtgatg ccgtttgttc
      181 acccagtacc agagccgggg aaaggcatac acgataaagg ccaggcggga cgcgatccgg
      241 aattccggat agaattctgc tgagaccatg cggtcatact cgctcaaatc ggcatgcccg
      301 tttttgaaga tgttcgtgag agtaatggcc gcaagctggc cgctccgtac cgcatagtag
      361 atgccttctc caaagaaggg gtccaccaag ctggccgcat cgcctgcaag gaggatgcgt
      421 tggcgggaca agacgggcgg cggtccctgg aagagcggaa tgggatgacc cattggtttg
      481 ggaattggaa gccccgttaa agacccttgt cctttcgtga agcgatgaaa ataatgatgc
      541 ggatcccacc gtttcatcct gaaaccggcg atgccgatcg agagtttatc ctgctttggg
      601 aagatccagc cgtaaccgcc cgggatacgt tcaaaatcaa ccagtacttt tcttcggaga
      661 tccaggtccg gcatcctgcc gaggggcagt tcgctttcca gcgaaatcgt cttgtaaacc
      721 ctccgatccg gaaagaggcg tcgggccacc atgctgttgg ccccatccgc tccgatgaga
      781 taacgcccgc ggtaccgtct ttgagcggtc cgtatctcca ccccttgaga gtggtccatt
      841 atctccaaaa cggtctctcc ttccacgata tgcgttcctg cccgtttggc tttcgaagcc
      901 aagagcgcgt caaagcgatc ccgcatgacc atgtaagcaa tcggcttgct ggattcaaca
      961 tggagatcat cgtttccttt aaaagtaaag tggacgccgt aaatggtctg ctccaccact
     1021 tctttaaagt ccggttccag gattttttcg atccgctggg aaagtccgcc gccgcagggt
     1081 ttgtatcggg gcatgctctt tttttcaaat gcgagaaccg agtaaccggc cttgctgagc
     1141 tcgtaggccg ctgtagaccc ggccggcccc aggcccacga tcaggacgtc gtaggtctgg
     1201 acgaaggatt ccgtctggag agtcatgaag cgattttctc tgtaatccgg gccgccaatt
     1261 tcctacctgt ctttagtctc ttccatcctg ctgatgatga cgatgacgtt ctgctccttg
     1321 ccgtcgcgaa ggatctttaa cgatgcctgt ttcccgattg gcgtgccggc tactaccacg
     1381 ggtaaatccc gcatgtggcc gatcttcttg ccattaaatt caacaatcac atctcccttc
     1441 ttaatcccgg ctaaagccgc cgggctgtcc tcaaacacat ccgagaccag ggccccttcg
     1501 cccttcttga tgccaaaaga ctttgccaga tcttttgtga attcctgaat catcactccc
     1561 agccagccac gagtcacctt gccttctttt tctaattgcc caattactgt cttgacctga
     1621 ttgatgggaa ttgcaaaccc gattccttga cccgaagggt tgatggcggt gttgattcca
     1681 atcacctctc ctcgaatgtc gaacagcgga cccccgctgt ttcccggatt gatcgaggcg
     1741 tctgtctgaa tgtaatcgtc ataaggaccg gacccgatcg cacggccttt agcgctcacg
     1801 atgccaaccg tgacggtttg ttctaacccg aacggattac cgattgccaa gacccattcg
     1861 cccacttctg cccggtccga gtctcctaat ttcacaaagg gcaacggttc ctgatttttg
     1921 atccggatca aggcaatatc ggtttttgga tctactccga tgagcttggc ctcaaactgc
     1981 cgcttgtctg ataacgtgac caggatcttt tcggcctttt caatcacatg ttgattcgtg
     2041 aggataaggc cgttcgggtt gatgataaaa ccggacccta aacttgtatc cttaaattca
     2101 cggggcaaaa catctccaaa aaattctcga aacggatggc tccggcccgg cagggtaccc
     2161 gctccttgaa cctcctggat gatgctaata ttgaccacgg ccggtttttc ttttttaaca
     2221 agttcagcga aagtgaaagc ccgaggttca ttatcttgtg cttctgcttc ggcgcgactc
     2281 ggtttcagac ttatctgaat cagcgtgccg atcaagagta aggcaaacaa acggtggcga
     2341 tctaaaaatt tattgtccca cataccaacc gataccgtgt tgtgacagaa acgccgtgat
     2401 cagggttaag gagttggtgt agatcatcac cccgaccaca atgagaaata agccgctgac
     2461 cgttgtgatc atccggagat gatggcgaac ctgtttaaag taagttaaga aagagttgac
     2521 cccgagtgat gtcaggaaga gcggaagccc cagac
//
LOCUS       DATMKH010000457         1215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2451076, whole genome shotgun sequence.
ACCESSION   DATMKH010000457 DATMKH010000000
VERSION     DATMKH010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2451076"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="VNV63_05530"
     CDS             complement(<1..402)
                     /locus_tag="VNV63_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62114.1"
                     /translation="MSQYRNPDNLPGPTGYSTSFISGTPLPYTHFGSLRIGSESVPHY
                     QELRQHMVRAREGQKTLAVVENRNDIETNLVLYSAKAAAEAGTPIEPRAGTLGKEAAG
                     LLPIDQGLLTNNGGRHSAATAAAHELAHLARM"
     gene            complement(725..>1215)
                     /locus_tag="VNV63_05535"
     CDS             complement(725..>1215)
                     /locus_tag="VNV63_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62115.1"
                     /translation="IRNPQTGVPEDPPRVPLPNWTQREERAAIKVENAVAKANGEPGD
                     RDSHGYAGFFFTDNITSTTPVDPLQKSIIEHFEPQLRTQTQLIDQLRTLPGNLHEPIP
                     ATRAHTTETARDFLDLYGNMSVINEMISSGKSPDEIATELQLTAAQKEMVKNMFSPPP
                     QP"
BASE COUNT          250 a          313 c          324 g          328 t
ORIGIN      
        1 catgcgagcg aggtgcgcta actcatgtgc cgctgcggtt gcagccgaat ggcgcccacc
       61 attattcgtt aagaggcctt gatcaatggg taaaagcccc gcggcttctt tcccaagtgt
      121 cccagctcga ggttcgatgg gagttccagc ctccgctgcg gctttggcac tgtatagaac
      181 taaatttgtc tctatatcat tgcgattttc cacgacagcc agagtctttt ggccctctcg
      241 cgccctcacc atgtgttgcc gcagttcctg atagtgcgga acgctctcac ttccgatccg
      301 tagagatccg aaatgcgtgt acgggagcgg cgtccccgaa ataaagctag tcgaatagcc
      361 agtcggtcca ggaaggttgt ccgggttgcg atactgactc attatctcct cgggaggagt
      421 ggtaggcgag taatcatctc ttatcccctc atcggcttcg gtcaacaatc tcggagggat
      481 tgtcagcgga ggaaggctgg agggcccagc cgtatgatcc ggtggttgtg aataagacga
      541 ctgctcttgc tctgctattt cttcgttagg aatacctact ccatatccgt gcgaaccatc
      601 accaatagaa cccgacattt ttctcctttg ctgagataac aggccttacg tcttacctat
      661 tcccgaagga gccggggtgg cttttatatc agcgctgaag gttgttgcct ctgatttttg
      721 cccatcaagg ctgcggagga ggcgagaaca tatttttgac catctccttt tgtgcagccg
      781 ttagttgcaa ctccgtggcg atttcatccg gcgatttccc ggaagatatc atctcgttga
      841 ttacagacat gtttccataa aggtctaaaa agtctctcgc tgtttcagtt gtgtgagccc
      901 gcgtggctgg gatgggctca tgcaaattcc cagggagagt tctcaactga tcgatcagtt
      961 gggtctgcgt ccgaagctgg ggttcgaaat gttcgattat gctcttctgt agtggatcaa
     1021 caggagttgt ggacgtgatg ttgtcagtga agaagaatcc ggcataacca tgtgagtcac
     1081 gatcccctgg ctccccattc gctttagcca cggcattctc gacttttatg gcggcacgct
     1141 cctctctttg cgtccaattc ggaagcggga cacgcggagg atcttcgggc accccggttt
     1201 gaggattacg aatcg
//
LOCUS       DATMKH010000458         3399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_533501,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000458 DATMKH010000000
VERSION     DATMKH010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533501"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(129..791)
                     /locus_tag="VNV63_05540"
     CDS             complement(129..791)
                     /locus_tag="VNV63_05540"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HXC62116.1"
                     /translation="MQRFLALNRILTDLGRYAQALRNLKLYAPEFLEKIKDDFPPDFW
                     WIAYPQGVRDAVEPIAQESQVNPDLVTAVIREESNYDPEAVSSVGALGLMQLMPATAQ
                     WVAKRINIESFSRDQLHSSSINVRLGAHYLAFLLDRFAGDPILAVAAYNAGPEAVTRW
                     EAVVPTEDRDEFLETIPFQETRSFVKRVMRSYYEYQLLQKSRQPDSSGINNNRDAFLD
                     KK"
     gene            1021..>3399
                     /locus_tag="VNV63_05545"
     CDS             1021..>3399
                     /locus_tag="VNV63_05545"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HXC62117.1"
                     /translation="MKEEMIKVAVVGGNQEGLETVALLTTDHQTQVMMVAEPNPSALA
                     YHLQDFGFAFADHHRFTVTQDLQALTTFPELHLIVDASGDPKVHRDLQQLSIPHAVIF
                     NGQCARFLWELRLIQGSDSERKAFVLERIKTAFDSIDLSRDWTELCELLIQVGLIAAA
                     ADGAWLYLSEVKQSHLILYPYRAIGSFEESARPPWAADLDVILANLARHSGEVCRIGP
                     DATEPMAKAVLEELSAGDLLVLPIKSEGDLLGVLELKRAKDKPPFQESDVTFLIQLAS
                     LAERYFKKMITLKDLQESSLREALQKELQEILSKDLPNKEKLSQGLRQITELLKVPWW
                     NLYVRNPATTDLVLQASNTVGPELIGVMKVKAGQGLAGEAVQTGRVLYFKETVENGLD
                     PAKRVSQVKGRRGAIFLPLVSGTGTVGLLELEWKDNELIQGATLKLLKEAAGLLANSI
                     MGDVERHRMSQKVIKLTAVNEEGLELLSTTDRDKVLRLVVSGAATILDAEAVILRLWD
                     SSAQRYRVASTYGLHQDEIDRALIDLDAHLAQPLRSIGTILAHEDLTALGLNLPANFP
                     YKSVLSAAFSWEEETAARMTASQKGPASGLAGQPLGILSAYNKLQYQSLACTAFDLDD
                     QEILTKYLHYMAKAIMNAQEHQARESLVTIDALTGLRNERYLDLRLPEEIQRAERYHR
                     SLSLLIMEVAHYKELTQTLSNSSRKEILRKIAGVVRETFRNVDILVRLKESRFAVLMP
                     DTGEKVVEAIVRLAKNIGGIKIKHRTSGQTTALQLQVGFSTFPQDAKTREELV"
BASE COUNT          797 a          905 c          924 g          773 t
ORIGIN      
        1 ttttgtacca ttgttgatcc ctgctgatcg gactcaacca ccatgcctgc catattcact
       61 gtcagaataa gtcctactct ctgactgcac tggtcctgtt tttagcacag caaactatac
      121 aattttactt attttttgtc aagaaatgca tctctattat tatttatccc ggaggaatcc
      181 ggctgacggg atttctgtag aagctgatat tcatagtagc tccgcatcac ccgttttaca
      241 aaactacggg tctcttgaaa cggaatcgtt tccagaaact catcccggtc ctcggtagga
      301 acaactgctt cccatcgagt caccgcctcc ggaccggcat tgtaagcggc tacggccaag
      361 atcgggtcgc cggcaaaacg atccagcaaa aaggcaaggt aatgagcacc gagtctgaca
      421 ttaatggaag aggaatgcag ctggtccctt gagaaggatt ctatgttgat ccgtttggcc
      481 acccattgcg cggtcgccgg catgagttgc atcagaccga gcgcaccaac ggaggagacc
      541 gcctctggat cataattact ctcctcgcga atcaccgccg tgactaaatc aggattcacc
      601 tggctctctt gggcaatcgg ctcgaccgcg tctcgcacgc cttgcggata ggcgatccac
      661 caaaagtccg gagggaaatc atccttgatc ttctccagaa attccggggc gtagagcttc
      721 aagtttcgca acgcctgggc ataacgtccg agatccgtca gtatccgatt gagcgcaaga
      781 aaacgctgca ttctatctcc cggaggggcc cgcgtatctc ccggagagaa ccgcttccct
      841 atctcctggt caggcaggcc tccagaacgg acagagcggt ttatcttcaa tgacgcctgt
      901 ccagccgggt cggcgtacag ggaaatctaa tctcccgaag ggttccgcaa attccccgga
      961 agatccgaag agctttcttg agcgaatgaa aaagttgggg ctggttaaag agctgacttc
     1021 atgaaagagg agatgatcaa ggtcgccgtg gtaggcggga accaggaagg gctggaaacc
     1081 gtcgccttat tgacgacgga tcatcaaact caagtcatga tggtagccga gcccaaccct
     1141 tctgctttgg cttatcacct tcaggatttt ggtttcgcct ttgcggacca tcaccgtttc
     1201 acggtgaccc aagatcttca ggctctgacc accttcccgg aactccattt gattgttgac
     1261 gcctccggtg atcccaaggt tcatcgagac ctccagcagc tttccattcc ccacgccgtg
     1321 atcttcaatg ggcaatgtgc tcgttttttg tgggaactaa ggttgattca ggggtctgat
     1381 tcggaacgca aggctttcgt cctggaacgg atcaagaccg cgtttgactc gattgacttg
     1441 tcgagagact ggaccgaatt atgtgagtta ttgatccagg tcggtcttat agcggccgcg
     1501 gcggatggag cctggctcta tctctccgag gttaaacagt ctcacctgat tctatacccc
     1561 tatcgagcca tcggttcctt cgaggagtct gcccgaccgc cttgggcggc ggatctggac
     1621 gtgatcctgg ccaacctcgc gcggcattcg ggagaggttt gccggatcgg gccggatgca
     1681 acagagccga tggcaaaggc ggtattagag gaactgtccg ccggggatct tttggtcctc
     1741 ccgatcaaat cggaagggga cctactcggt gtcctagagc tcaagcgagc caaggacaaa
     1801 ccgcctttcc aagaatcaga cgtgaccttt ctaatccagt tggccagttt ggccgaacgc
     1861 tattttaaaa agatgatcac cttgaaagac ctccaggaat cctctctgcg agaggcgttg
     1921 caaaaagagc ttcaagagat tctgagcaag gatctcccta acaaggagaa actctctcaa
     1981 ggtctccgtc agattacaga actcctcaag gtgccttggt ggaatctcta tgtgagaaac
     2041 ccagccacca cggatctggt tctccaggca tcgaatacgg tcggcccgga gttgatcggg
     2101 gtgatgaagg tcaaggccgg tcagggcctt gcaggggagg cggtgcagac cggacgggtc
     2161 ctttatttta aggaaacggt agagaatggc ctcgacccgg cgaagcgggt gagtcaggtt
     2221 aagggccgtc ggggagcaat cttccttccg ctcgtcagcg gtacgggaac ggtggggctg
     2281 ctcgaactcg aatggaagga caatgaactt attcagggag caacccttaa gcttctgaag
     2341 gaagcggcag ggctcctggc caattcgatt atgggagacg ttgagaggca tcggatgagt
     2401 cagaaggtca ttaagctgac ggcggtcaat gaagagggcc tagagctgct ctctactacg
     2461 gatcgtgaca aggtcctacg actggtcgta tccggtgcag cgacgatcct ggatgccgaa
     2521 gcggttatcc tccgtctttg ggattcgagc gcgcaacggt accgggtggc ctcgacctat
     2581 ggtctccacc aggatgagat tgaccgagcg ttaatcgatc tggatgccca tcttgcacag
     2641 ccgctccgtt ccatcgggac gatcttggcc catgaggatc ttaccgcact gggtttaaac
     2701 ctgcccgcga attttccata caagtcggtt ttgagcgccg ccttttcttg ggaggaggaa
     2761 actgccgcaa gaatgactgc cagtcaaaaa ggtccggcaa gcgggctggc tggacagcct
     2821 ttgggtatcc tgtccgccta caacaaactc cagtaccagt ctttggcctg caccgccttt
     2881 gatctcgatg atcaagagat actaaccaag tatctccact atatggccaa ggcgatcatg
     2941 aacgcacaag agcatcaagc ccgagagtcc ttggtgacga ttgatgccct caccggtctc
     3001 cgcaacgaac gttacctgga tctccggctt ccggaggaaa tccagcgggc ggagcgctac
     3061 catcggagcc tttccttgct catcatggaa gtcgcgcatt acaaagaact cactcagacc
     3121 ctgagcaact catcgcggaa agagattctc cggaagatcg caggtgtggt acgcgagacc
     3181 tttcgtaatg tagatatctt ggtcaggctg aaggagtccc gtttcgctgt tctgatgccg
     3241 gatacgggag aaaaggtggt ggaggcaatc gtccggctgg ccaaaaatat tggaggcatc
     3301 aaaatcaagc accggacctc cgggcagaca accgcccttc aactgcaagt gggtttcagc
     3361 acttttcccc aggatgcaaa gacccgggag gaattggtg
//
LOCUS       DATMKH010000459         1405 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_534011,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000459 DATMKH010000000
VERSION     DATMKH010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1405
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534011"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1178)
                     /locus_tag="VNV63_05550"
     CDS             complement(<1..1178)
                     /locus_tag="VNV63_05550"
                     /inference="COORDINATES: protein motif:HMM:NF023855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching enzyme N-terminal
                     domain-containing protein"
                     /protein_id="HXC62118.1"
                     /translation="MQIELGPDILQDYEKASKREWLVTNGIGGYSSSSLAGSNTRKYH
                     GLLVAALSPPTGRVVTLSKLEETFLVNGQAFPLSANQYPGALSPQGFRFLSLFKRYPV
                     PTFLYRPTQDIEIEKSIWMAPGKNTTYIRYTLLRSPTEARIRLLPLMAWMDYHAEFHR
                     REDFPFSVSWQDPELRVTPYAGVAPLRLRFEHARFFSMPDWYYNLEHLRELERGLDFS
                     GDLYSPGYFERDLSPGDSIIFAATVESSIENGEAVLASVVEQQQRLITRAGVSDDFGK
                     MMVLAADQFIIHPPRGSNIRATVIAGYPWFTDWGRDTMISLPGLCLSTGRPETAREIL
                     AAFAGFISKGMLPNRFPDAGQDPEYNTVDAALWYFQAVYNYLNKTDDADFIGNYLLPG
                     L"
     gene            complement(1205..>1405)
                     /locus_tag="VNV63_05555"
     CDS             complement(1205..>1405)
                     /locus_tag="VNV63_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-nucleotide phosphodiesterase"
                     /protein_id="HXC62119.1"
                     /translation="LKGLAVRSKHLTPALLLKEFSKIGKPRLPLFIYHMKPRYLDAIR
                     RELKNLKIKKMIILKDGMVVDI"
BASE COUNT          344 a          365 c          378 g          318 t
ORIGIN      
        1 agccccggga gcaagtaatt cccgataaaa tcggcgtcat cggttttgtt aagataatta
       61 tagaccgcct gaaaatacca gagcgcggca tccacagtgt tatattccgg atcttgcccg
      121 gcatcaggaa agcggttagg cagcatccct ttgctaataa aaccggcgaa tgcggccagg
      181 atctcacgcg cggtctcggg cctgccggtt gagagacaga gtcccgggag cgagatcatc
      241 gtgtcccggc cccagtcggt gaaccagggg taacctgcga tgaccgtggc gcgaatattc
      301 gaaccgcggg gaggatggat gataaattgg tctgcggcca ggaccatcat cttcccgaag
      361 tcgtctgaga cgcctgcccg tgtgatcaaa cgctgctgct gttccaccac ggaggccagc
      421 accgcctcgc cgttttctat cgaagattct accgttgcgg cgaatatgat gctgtctccc
      481 ggagaaagat cccgttcaaa gtaccccgga gaatacaggt ccccgctgaa atcaagacct
      541 ctttccaact ccctgagatg ctccaagttg tagtaccagt caggcattga gaagaaccgg
      601 gcatgttcga atctcaatcg cagcggtgct acaccggcat agggagtgac ccgaagctcc
      661 ggatcttgcc aggaaacgga gaaaggaaag tcttcgcgac gatgaaactc cgcgtggtaa
      721 tccatccacg ccattagcgg gagcaagcgt atccgggcct ccgttggaga ccgaagaagt
      781 gtataacgga tgtaggttgt gttcttcccg ggcgccatcc agattgattt ttcaatttcg
      841 atgtcttgtg tcgggcgata aagaaaagtc ggcaccgggt atcgtttgaa caaagacaga
      901 aacctaaatc cctgcggaga aagtgcgccc ggatattgat tggcgcttaa tggaaacgcc
      961 tgcccattga ccagaaaagt ttcttccagt ttactgagcg tcaccacgcg gccggtggga
     1021 ggagacaggg cggctacaag aagaccgtga tactttcgag tattggagcc ggccaatgag
     1081 ctcgatgagt atcccccgat cccgtttgtg accagccatt ctcttttgga ggctttctca
     1141 tagtcttgca gaatatcagg tcccagctcg atctgcattc agctcacatc agagggatga
     1201 gcgtctagat atcaacgacc atcccatcct tcaagataat cattttcttg atttttaaat
     1261 tcttgagttc tcgccggatc gcatcaagat agcgcggttt catatggtag ataaacagcg
     1321 ggaggcgagg cttcccgatc tttgaaaatt ccttaaggag aagtgcaggg gtcaggtgct
     1381 tactcctcac ggccagaccc ttcag
//
LOCUS       DATMKH010000460         1803 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2783226, whole genome shotgun sequence.
ACCESSION   DATMKH010000460 DATMKH010000000
VERSION     DATMKH010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2783226"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VNV63_05560"
     CDS             <1..342
                     /locus_tag="VNV63_05560"
                     /inference="COORDINATES: protein motif:HMM:NF012687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase"
                     /protein_id="HXC62120.1"
                     /translation="GDRGKIKRFASAYVPMDETLAFAAVDLVKHRYFVLSNFELKRNS
                     VEDLAKEDASHFFHSLCDSLQCTMHLRIDYGSNDHHKLEALFKALALSLRSASEIDPR
                     RQAVASSKGSM"
     gene            351..1004
                     /gene="hisH"
                     /locus_tag="VNV63_05565"
     CDS             351..1004
                     /gene="hisH"
                     /locus_tag="VNV63_05565"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HXC62121.1"
                     /translation="MCALIQILDYGSGNLFSVKHSLHRASPELEVAISSDYEKGKVDG
                     LILPGVGSFPSAQKILGKNRKAILHDIAERKLPALGICLGMQLMFEESEEGKGKGLGL
                     FPGKVKRFQTASGVKVPHMGWDKVEVKSQATKQSEYSMGLPSKGWGYFAHSFFPEEVV
                     NSEVIAFTKYGHQRFASIIQNGNILGTQFHPEKSGDFGFRIISNFAMRVLARSGGAI"
     gene            1004..1735
                     /locus_tag="VNV63_05570"
     CDS             1004..1735
                     /locus_tag="VNV63_05570"
                     /inference="COORDINATES: protein motif:HMM:NF013168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]
                     imidazole-4-carboxamide isomerase"
                     /protein_id="HXC62122.1"
                     /translation="MIVIPAMDLLEREVVRLEQGDIRNPTVYSGDPSEIVQSFVSHGA
                     SRIHLVDLNAAVLSDPVTNAPIIDGLLHQAGESIAFQVAGGIRNTSFAASLLARGASS
                     IVLGSIAYSEPEGAKEILSSLGPSHVILALDYDRSGCVRTRGWGKSENEKVETAFHRF
                     FDLGFRQFLFTSIDRDGLMTGPDLETLARLKKVASSNSKIIGSGGVASIEDIANLRKL
                     DLDGVVVGKAIYENSIPLSILGEES"
BASE COUNT          456 a          427 c          482 g          438 t
ORIGIN      
        1 ggagatcgag gcaagatcaa gaggtttgct tccgcatatg tcccgatgga cgaaacactc
       61 gcttttgcgg ccgtggatct ggtaaagcac cgttattttg tgctctcgaa ttttgaacta
      121 aaaagaaact cggtggaaga cttggcaaaa gaagatgcgt cccacttttt tcactccctg
      181 tgtgactctc tgcagtgcac catgcacctt aggatcgatt atggctctaa cgaccaccac
      241 aagctggaag ccctcttcaa ggccctcgcg ctctcgctca gatctgcctc cgaaatagat
      301 ccccgcaggc aggcggttgc aagctccaag ggatccatgt aggctgtccc atgtgcgccc
      361 ttattcaaat actggactat ggttcgggaa atctgttcag cgtcaagcac tcgctgcacc
      421 gggcttctcc ggaactcgag gtcgcgatct catcggatta tgagaaagga aaagtggatg
      481 gactgatcct ccccggagta ggaagttttc cttccgcgca aaagatcctg gggaaaaaca
      541 ggaaagccat tctccatgac attgcggaga gaaagttgcc agctctcggc atttgcctcg
      601 gcatgcaact catgtttgaa gaatctgagg aagggaaagg aaagggtctc ggcttattcc
      661 cgggaaaagt gaaacgcttc caaacagcca gcggagtgaa ggttcctcac atgggctggg
      721 acaaggtgga ggtaaaatcc caagccacga aacaaagcga gtattccatg ggtcttccat
      781 ccaaaggatg gggctatttc gcgcattctt tttttccgga agaggtggtt aattcagaag
      841 tgatcgcctt caccaagtac ggacaccaga gatttgcctc gattattcag aatggaaaca
      901 ttctgggaac acaattccat cccgaaaaat ccggggattt tggctttcgg ataatttcca
      961 attttgcaat gcgagtgttg gctcgctcag gaggagcgat ctgatgattg tgattcccgc
     1021 gatggatctc ctggaaagag aagtcgtcag gttggagcag ggcgacatcc gaaatccgac
     1081 agtatattcg ggcgacccat cggaaattgt gcagagtttc gtgagccacg gcgcttctag
     1141 aattcacctt gtagatctaa atgcagcggt actttcggat cctgtgacga atgctcccat
     1201 cattgatgga ttgctacacc aggcagggga atcgatcgct tttcaggtcg ctggcggtat
     1261 tagaaataca tcctttgccg cgtcactgtt ggcgcgaggc gcgagcagca tcgtcctcgg
     1321 ttccattgct tattcagagc cggaaggagc taaggagatc cttagctctc tcggaccaag
     1381 tcatgtaatc ttggcactgg attacgatcg atccgggtgc gtgagaaccc gcggctgggg
     1441 aaagtcggag aacgaaaaag tagaaactgc ttttcacaga ttcttcgatt tgggctttcg
     1501 ccagttcctg tttacttcca ttgatcgcga tggcttgatg actggccccg atctggaaac
     1561 gctcgcacgg ttgaaaaaag ttgcatcttc gaactcgaaa atcataggct ccggcggagt
     1621 ggcctccata gaggacattg cgaacttgag aaaactcgat ctggatggag tggtcgtcgg
     1681 aaaagcaatt tatgaaaatt ccatcccgct ctcgattctg ggggaagaaa gttagaaact
     1741 tgcccttagc caagcggatc attccttgcc tcgatgttaa gaatggcgaa gtggttaagg
     1801 gaa
//
LOCUS       DATMKH010000461         1130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3099951, whole genome shotgun sequence.
ACCESSION   DATMKH010000461 DATMKH010000000
VERSION     DATMKH010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3099951"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="VNV63_05575"
     CDS             complement(<1..423)
                     /locus_tag="VNV63_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008098289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62123.1"
                     /translation="MTFSSRNAKFTLAYGLSRSVMARIYIKTYGCQMNERDSEQVAQM
                     LVARGHVLTSQEEEADVVLLNTCSVRDLAEQKALGKMGMLGSLSRQRPIVFGFLGCMA
                     QSRGESLLRSVPHLNLVVGTQKFHRVADYVDQALAHQAE"
BASE COUNT          270 a          312 c          295 g          253 t
ORIGIN      
        1 ttcggcctga tgtgcgagcg cctgatcaac atagtcagcc actcgatgaa acttctgggt
       61 tccaaccacc agattcagat ggggcacact tcggagaagt gattctcccc ggctctgcgc
      121 catacacccg agaaatccga aaacgattgg tcgctgccgg gaaagagagc ctagcatacc
      181 catcttcccg agagcctttt gttccgccaa atcccggaca ctgcaggtat tcagcaagac
      241 cacatcggcc tcttcctctt gcgaggtcaa aacatggcca cgggcgacca gcatctgagc
      301 gacttgttcg gagtcgcgct cattcatctg gcatccgtaa gttttgatat agatcctggc
      361 cattacagat cgcgaaagtc cgtaagctaa cgtgaattta gcgttgcgcg acgaaaaagt
      421 caccctggta gggatgagca gccgctcatc cgagacccgt cccggaggga ctaaatgatc
      481 gtagccaggc aatttattgc ctggaacccg ttcaatcgag aatccgttcc gtagggcacg
      541 gtctgatcct gattccgggt tgatgagtcg ccctgatcgt agcacgccta tcggacctaa
      601 tcataacgtg ccctacggga cgattccggg tttcgcgtga ataccaggca ataaattcct
      661 ggctacgatc attctgtccc tcctccggga cggggacacg gatgagcggg agcatcggtt
      721 tttgtcccga agggaccaaa ggactaggcc cggggtttta accccgggtt atcgacgcaa
      781 aaaagggccc gcccggaagg ggcggtagag tcggtgttat acaatcaggc gccgaacaaa
      841 atctgggagg aattatctgc cgccccttca gggcggtttc atgaggtgaa tctttcccgg
      901 ggttaaaacc gcgggctaag ttctgtaatc cctacgggat aaatttgcca aactcccacc
      961 ccgctctctg atttcaccaa atccaaccga tcggcttgaa accgatcaca gactggaaga
     1021 agccttagaa gcgagttcca agcgaactcg ttgaaaggtc acttccatga aaagcttctt
     1081 cgcatcgttc ttcggtacga tcgccggtat ttgcgtattg ataggcttcg
//
LOCUS       DATMKH010000462         4007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2783574, whole genome shotgun sequence.
ACCESSION   DATMKH010000462 DATMKH010000000
VERSION     DATMKH010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4007
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2783574"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="VNV63_05580"
     CDS             <1..420
                     /locus_tag="VNV63_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D ubiquinol oxidase subunit II"
                     /protein_id="HXC62124.1"
                     /translation="DEPGGSYWKEWKTYIEQQLVKRGMISPDDMNLFTVTDQVEEAVK
                     EIANFYRVYHSARHVDERLVIRLNRSLSPDVIERLNSEFRDILTEGKIEAGEPQAQEI
                     REKELLHLPRLLLKFNRTSLGRLRQLINAINQAPPSS"
     gene            complement(447..1295)
                     /gene="hpnD"
                     /locus_tag="VNV63_05585"
     CDS             complement(447..1295)
                     /gene="hpnD"
                     /locus_tag="VNV63_05585"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HXC62125.1"
                     /translation="MRSKQFGASITKKSGSNFYYSFLFLPKVQRRAIYTVYAFCRLVD
                     DAVDSRPSHSEAAAELSTWRKELDQCYHGQPNHPLSRELQPIIRQFEIPQDYFQELLN
                     GIEMDLIKKRYATFSELSEYCYRVASVIGLICIRIFGCDTQTVREHAIHQGMAFQLTN
                     VLRDLGTDADRGRIYLPLDELARFGYSEAELLRSTYNPAFVELMRFQTQRAKRYYEKA
                     KSLIPREARRALVASEIMGAIYENLLDVIEERRYRVFDERISLSTIRKASLALQAYLK
                     NRSAAV"
     gene            1596..3095
                     /locus_tag="VNV63_05590"
     CDS             1596..3095
                     /locus_tag="VNV63_05590"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXC62126.1"
                     /translation="MSIPYLKFNRHIVLALLLVFLWVFVQHRVFQTDGRFFQPSFSGI
                     KGLLLYEMGDYSGAAKAYRAHFREAKAYRSNFQEVYQADRAPEDPVWEAFLQGDFQAA
                     REISEKALEKNPSAIGPFLNLGEIAMEEGAPDQALDVFNRVLQKQADQFDALLLSSVA
                     YARTGDYKKAISMINRALRSNRTESRITAFLKVLEVTGDLSELPENQKPLCLLAHYYR
                     YLQVFDSSNGKIAAAYAVQAIASGDQPDDAYLTLGAIYRREGKEEKALQAFLKAMELN
                     PKNTEAYRLAVQIYSERGDLLNEYRIRKAAYEMAQADPFYAVSFSHFLSEKLEDYTQA
                     LAVTEKLLETTPNDSAVLHQAGDLYRLTDNPKRSMEYYRKALQLKPQNPSLHEGMGHG
                     MSELGKKNDAMKAYRTAMAIDPRRTQPHLGLAGIYLDGRRYREAIKEYEAADRLGKVE
                     ASERTPLCVLYHQVSEFKRAADCFKQILSEDPHNAVAHYLLPYTLKILR"
     gene            3092..>4007
                     /locus_tag="VNV63_05595"
     CDS             3092..>4007
                     /locus_tag="VNV63_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62127.1"
                     /translation="MRKTKENKFRVRSLVKNVSGYLALRYTFIIGICLLVATVQLNYR
                     VPSSFLFLALPGIGVALSVLGFSLVFKQLMAGVPSYHPFSQALQWIEGGAYLLVSVFV
                     LSSILLYANGRLDHSLPVNRSSEILTISGGQINLGLKIPYVWVSLRSWEDPDRTERLL
                     LKRGEQRVLWGGQPVVIQIQHGYFGIPWAFNIQPDEEKYNLEILKLAPTALAVRKKLI
                     NFYLDHQRWEEAAAMTDDYLKIYPNDGAFALEVGSVLNSADEYGQGIQFLDHVVQRRP
                     TYEACQLLGAALSHQGKKTRAAEVFNESI"
BASE COUNT          969 a         1009 c         1069 g          960 t
ORIGIN      
        1 gacgaaccgg gagggagcta ctggaaggaa tggaaaacat atatcgagca acaattggtc
       61 aagagaggga tgatctcacc cgatgacatg aatctcttta ccgtcacgga tcaggtagag
      121 gaagcggtaa aggagatcgc gaacttctat cgcgtctatc actccgcccg tcatgtggat
      181 gagcggttgg tgattcgtct gaaccggtct ctttctcctg atgtaatcga acggttgaac
      241 tcggagttcc gggatatttt aacggagggg aagatcgagg ccggcgagcc gcaagcccaa
      301 gagattcgcg agaaggagct gcttcacttg ccgcgtctat tgctcaagtt caaccggaca
      361 agccttggtc gtctccgtca gcttatcaac gcgatcaatc aggccccgcc ttcttcgtga
      421 tatcatgaag tcagcacatc ctgctttcat acggcagcgc tgcggttttt taaataagcc
      481 tggagtgcaa gagaggcctt ccggattgta gaaagggaga tcctctcgtc gaacacccgg
      541 tatcgccgtt cttcgatcac atccagcaga ttttcgtaga tcgcccccat gatctctgag
      601 gcgaccagtg cgcgccgggc ctccctcggg atcagcgact tggccttctc gtagtatctc
      661 ttagcccgct gagtttgaaa tcgcatcaac tccacaaagg caggattata ggtcgatctg
      721 agcagctcag cctcgctgta tccaaagcga gccagctcat ctaaaggcaa gtaaatcctt
      781 ccgcggtcgg catccgttcc aaggtcacgc agtacgttgg tgagctgaaa ggccatgccc
      841 tgatggatcg catgctcacg cacggtttgt gtgtcgcagc cgaaaatccg tatacagatc
      901 agtccaataa ccgaggcgac ccgatagcag tactcggaca gctcgctgaa agtagcatag
      961 cgttttttga tcaggtccat ctcgattccg ttcagaagct cttgaaaata gtcctgcggt
     1021 atttcaaatt gacggatgat cggctggagc tccctgctta gcgggtgatt cggctggcca
     1081 tggtagcact ggtctagctc cttgcgccag gtagaaagct ctgcggccgc ttctgaatgt
     1141 gacgggcggc tgtccaccgc atcgtccacc aagcggcaga aggcatagac agtgtagatt
     1201 gcacgacgct ggacctttgg aagaaaaaga aaagagtagt aaaaattcga accgcttttc
     1261 ttagtaatgc ttgccccgaa ttgtttagat cgcatcccgg ttgagttcac aatgacagtc
     1321 aaatttatgc gtcattaaaa catattccat cattttaagt caaacggggc ctctatggga
     1381 taaaccgggg ccttatcgta taaaaaccgg atcccttcag gattaagtac gggtccctcg
     1441 gggataaata cgggcccctc ctggataaat aatagatttt tacctgcttt ttgacaggaa
     1501 cgggaagcgg agttacactt cctagaggtc acttggtctt ctacagagta taaaggcact
     1561 acacgggaac ataacagact gtctacgagt ataacatgag cattccttac ctcaagttca
     1621 accggcatat agttctggcc ttgttgctgg tcttcctctg ggtcttcgtt caacaccgtg
     1681 tcttccagac agatggccgc ttcttccagc cctcattctc gggaatcaag gggttgctgc
     1741 tctacgaaat gggagactat agcggagcgg ccaaggctta tcgcgcccac ttccgggagg
     1801 cgaaggccta tcgctccaat ttccaggaag tctatcaagc ggatcgagct cccgaagacc
     1861 cggtatggga ggccttcctc caaggtgatt tccaagcggc gcgggagatt tcagagaagg
     1921 cgctagagaa aaatccatca gccatcggcc cgtttctgaa tcttggggag attgcaatgg
     1981 aagaaggtgc cccggatcaa gcgctggatg ttttcaatcg tgttcttcaa aagcaggccg
     2041 accaatttga tgcccttctt ctctcttccg tggcctatgc ccgcacgggt gactataaga
     2101 aggcaatcag tatgataaac cgggcattgc gcagcaaccg aactgagtcg cgaattaccg
     2161 cttttctcaa ggtcttggaa gtcacgggtg atctatcaga gctgccggag aatcagaagc
     2221 cgctctgcct gcttgcccat tattaccgct atcttcaagt ttttgattca tccaatggaa
     2281 agatcgcggc tgcctatgcc gtgcaggcca ttgcgtccgg cgatcagccg gatgatgcct
     2341 atttgaccct cggggcgatc tacagaagag aggggaagga agaaaaggcg cttcaagcat
     2401 ttctcaaggc aatggagctg aaccctaaaa acacggaggc ttaccgatta gctgtccaga
     2461 tctattcaga gcggggagat cttctcaatg aatacaggat cagaaaggct gcctatgaga
     2521 tggcacaagc ggatcccttc tatgccgttt cttttagcca tttcctttcg gaaaagctgg
     2581 aagactatac tcaggcgttg gctgttactg agaagctgct tgaaaccacg ccgaacgata
     2641 gcgctgtgct tcatcaagca ggagaccttt accgtttgac ggacaatccg aaacgttcga
     2701 tggaatatta ccgtaaggcc ctccaactga aaccacagaa cccatccctt catgaaggga
     2761 tggggcatgg catgtcagag ctggggaaaa agaatgatgc aatgaaagcc tacaggaccg
     2821 ccatggcgat cgacccgcgc aggacccagc cccatctggg tcttgccggg atctatctgg
     2881 atggacgccg ttatagagag gcgatcaaag agtacgaagc ggcagaccgg ttgggaaagg
     2941 tggaagcctc cgagcggact ccgctctgcg tcctctatca tcaggtctca gagtttaagc
     3001 gtgcagcaga ctgtttcaaa caaatcctct cggaggatcc gcacaacgcg gtggcgcact
     3061 atttgcttcc ctataccttg aagatattgc gatgagaaaa accaaagaaa acaagtttcg
     3121 ggttcgctcg ctggtaaaaa acgtttccgg ctatttggcg ctaagataca cgttcatcat
     3181 cgggatctgt ttattggtcg caacggtgca gttaaattac cgggttccgt ccagctttct
     3241 ctttttagct cttcccggga tcggtgtcgc gctaagcgtt cttggtttta gccttgtgtt
     3301 taagcagctg atggcaggag ttcccagcta ccaccccttt agccaagccc tccagtggat
     3361 tgaagggggc gcgtacctgt tggtcagtgt ttttgttcta tccagtattc ttctctatgc
     3421 gaatggcaga ctggatcact ccctaccggt gaatcggtcc tcggagatct tgacgatctc
     3481 cggcggtcaa ataaacctcg ggctgaagat tccctacgta tgggtgagcc tgcggtcttg
     3541 ggaagatccc gaccggacag agcgacttct ccttaagcgg ggggaacagc gcgttttatg
     3601 gggagggcag ccggtggtaa ttcagatcca gcatggttat ttcggtatcc cgtgggcttt
     3661 taacatacag ccggatgagg aaaagtataa tttggaaatc ctcaagctgg cgccgaccgc
     3721 cttagcggtg cggaagaagt tgatcaattt ctatcttgac catcaacggt gggaggaggc
     3781 cgccgcgatg actgatgact acctcaagat ctatccgaat gatggcgcct ttgcccttga
     3841 agtcgggagc gtcctcaaca gcgcggacga gtatggtcag ggtattcagt ttttagatca
     3901 tgtcgttcag cggagaccga cgtacgaggc ctgtcagctt ctgggcgcgg ccttaagcca
     3961 tcagggtaaa aagacgcggg cggccgaggt ctttaatgaa tccattc
//
LOCUS       DATMKH010000463         2535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_216694,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000463 DATMKH010000000
VERSION     DATMKH010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216694"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2020)
                     /gene="glyS"
                     /locus_tag="VNV63_05600"
     CDS             complement(<1..2020)
                     /gene="glyS"
                     /locus_tag="VNV63_05600"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HXC62128.1"
                     /translation="MSKDLIFEIGTEEIPANFIPPALDEIMEKGRQSFENQRIAFDKI
                     QAWGTPRRLVLSIIGLSETQAESSSEVIGPPKAVAYDATGQPTAAAIGFAKAQSVALS
                     ALQIRKTDRGEYLCAVKRAPRQATSKLLKILLPQLISSLTFPKTMRWNAEGIRFVRPI
                     RWIVVLYGDLVIPFRYAGVASGRLSYARRALGKKPVAVKNASSYQKQLKDHGVVVSVD
                     DRRAGVTTQLERLARQMSGVLPRDEELLEQTVFMVEEPVTLCGNFDPVFLRLPKEVLI
                     SAMREHQGYFHLRDRKDQLLPHFLCVVDGKAAGRVVQTGHERVLRARLSDARFFFEAD
                     QKRKLVDLVDELKKVTFQESLGTLYDKTERLMALSAYLALELDPALKEIVHRAAYLSK
                     ADLLTGMVGEFPKLQGIMGREYARIQGEPGAVAVAIGEQYLPRFAGDELPAGMAGKIL
                     SLADKMDTIAGCFGVGLVPTGSEDPYGLRRQAFGILQILVQGKHRLSLTVFVNEAIKH
                     LKNRIKKDPAQLQRELRDFLQQRLESHLLSEVLRYDLIGAVLARRADDPYDVTLRANA
                     LARFRSQPGFKPLVLAAKRLSNILKETGAGEVHPEFFKESAENELYDSLTKTQPLVEA
                     KIREQQYDDVLRLLTKLHEPIQLFFEKVLVMAPEDQIRQNRLALLQQVR"
     gene            complement(2039..>2535)
                     /locus_tag="VNV63_05605"
     CDS             complement(2039..>2535)
                     /locus_tag="VNV63_05605"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HXC62129.1"
                     /translation="FTYFQEVGGISLDPVTIEITYGLERIAMYLQGVDNVYDLRWNEN
                     VTYHEIHHEDEVQFSRYNFEAADVTMLMEAFRMFEAECRRLIAQGLTLPAYDYCIKTS
                     HLFNLLDARKAISVTERTGYIARVRGLARLCAERYLSDREAKGFPLLKKSAAPRQRVR
                     NLTS"
BASE COUNT          592 a          718 c          641 g          584 t
ORIGIN      
        1 ccctgacctg ctgaagaagg gccaggcgat tttgtcggat ctgatcttcc ggggccatga
       61 ccagcacctt ctcaaaaaac agctggatcg gctcatggag ttttgtcaat aaacgaagga
      121 cgtcatcata ctgctgttcg cgaatcttcg cttctacgag cggttgtgtc tttgtcaaag
      181 aatcataaag ctcattctcg gcggactcct tgaaaaactc cggatggacc tctccggcgc
      241 cggtttcctt caaaatattg cttaaccttt ttgcggccag cacgaggggt ttgaaaccgg
      301 gctgggagcg aaagcgcgcc agggcattcg cccgaagcgt aacgtcataa ggatcatctg
      361 cgcgtcttgc aagcaccgca ccgatcaggt cgtaccggag cacttccgat aaaagatggg
      421 attcgagacg ctgttgcaaa aaatccctca gctcacgctg aagctgggcg ggatcttttt
      481 taatccggtt tttgagatgc ttgatcgctt cgtttacgaa tacagtaaga gaaagacggt
      541 gcttcccctg aactaaaatc tgaagaatgc cgaaagcctg acgcctcagg ccgtagggat
      601 cctccgaacc ggtcgggacg aggccgacgc cgaaacaacc ggcaatcgtg tccatcttat
      661 ccgccaagga tagtatctta ccggccatgc cggccggcag ctcgtctcct gcaaaccggg
      721 ggagatactg ctccccgatc gcgacggcaa ccgctccagg ctcgccctga atcctggcgt
      781 attcccttcc catgatgcct tgaagctttg gaaactctcc caccattccg gtcaagagat
      841 ccgccttgga gagataagcg gcacggtgga caatttcttt taaggccgga tccaattcca
      901 aagccaaata agctgagagg gccattagtc gttctgtctt gtcgtacagt gtccccaggc
      961 tttcctgaaa cgtgaccttt tttaactcgt ccacgagatc cacgagcttc cgcttctgat
     1021 ccgcttcaaa gaagaacctc gcatcggaca gcctcgccct gagcaccctt tcatgaccgg
     1081 tctggaccac acgccccgcc gccttcccgt ccacaacgca gaggaaatgg ggaaggagtt
     1141 gatctttccg gtcccgaaga tggaagtagc cttgatgttc gcgcatcgcg ctgatcaaga
     1201 cctcttttgg aagacggaga aaaaccgggt caaaatttcc gcaaagagtc accggctctt
     1261 ccaccataaa gacggtttgc tccagcagct cctcgtctcg gggaagaacc ccggacattt
     1321 ggcgcgccag ccgctcaagc tgcgtcgtga cgccggcccg gcggtcgtca acagacacaa
     1381 caacgccgtg gtctttgagc tgcttttgat aagaagaagc gttcttgacg gcaaccggtt
     1441 ttttccctaa cgccctgcgg gcgtaactaa gcctgccgct tgcgacaccg gcatatcgaa
     1501 atggaatcac aagatccccg tacaacacta cgatccaccg gatcggccgt acaaagcgta
     1561 tgccttccgc attccaacgc atcgtcttag gaaaggtaag gcttgaaatc agctggggca
     1621 ataaaatctt tagtaattta gacgtcgcct ggcggggcgc tcgctttaca gcgcagaggt
     1681 actctccccg atcggtcttt ctgatctgaa gcgctgagag agcgacgctt tgagccttgg
     1741 cgaatcctat cgccgcagcg gtcggctggc cggtcgcatc ataggcaacc gctttgggcg
     1801 gacctatgac ttcggagctg gattcggcct gggtttcgct caatccgata atcgagagaa
     1861 ccagacggcg cggagttccc catgcctgaa tcttatcgaa ggcaatgcgt tgattctcaa
     1921 acgattgccg tcctttttcc ataatctcat ccagggccgg gggtataaaa ttcgccggga
     1981 tctcttcggt tccgatctca aatatcaaat ccttagacat agtttgcagg ctcgcgcttc
     2041 aacttgttaa attacggact ctctgacgcg gcgcggcgga ttttttgaga agcggaaacc
     2101 cctttgcttc acgatccgaa agataccgtt ccgcgcataa acgtgcaagt cctctcacgc
     2161 gtgcaatgta gccggtgcgt tccgtaacgc tgatcgcttt ccgggcatcc aggagattaa
     2221 aaagatgcga ggtcttgatg cagtagtcgt aggcaggcag tgtgagccct tgagcaatca
     2281 atcgcctgca ttcggcttcg aacatacgaa aggcctccat gagcatcgtg acatccgcag
     2341 cctcgaagtt ataccgggag aattgaacct catcttcatg atggatctca tgatacgtca
     2401 cattttcatt ccagcggaga tcatatacat tgtccacgcc ttgaaggtac atcgcaatcc
     2461 gctccaagcc gtaggtgatc tcgatcgtca cgggatccag gctgattcct cccacctcct
     2521 gaaaataagt aaact
//
LOCUS       DATMKH010000464         1192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3723401, whole genome shotgun sequence.
ACCESSION   DATMKH010000464 DATMKH010000000
VERSION     DATMKH010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3723401"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VNV63_05610"
     CDS             complement(<1..188)
                     /locus_tag="VNV63_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62130.1"
                     /translation="MNEFALPRAILDFDHHTFVAWNPRFLEQTGFSEDEMKNSKSEQL
                     LTLGDSWFPISDDRGGQA"
     gene            471..>1192
                     /locus_tag="VNV63_05615"
     CDS             471..>1192
                     /locus_tag="VNV63_05615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HXC62131.1"
                     /translation="MNLAVEAADIGTWEFDLEQGTGFISERCAQIMGYPKVSSSGMIQ
                     FSDWLSMIHWEYRRRINQACDPEGDGELKMRLQLIDTGGVIRHVLMRGRAFFAVSHRT
                     GSSPFRKAVRLLGIVRNLSDRHIYQRALAESDQRLRWALQHAPIPIMIHLDDGQIIEL
                     NRAWQRITGYSIEEIPTLDAWAAVSWDPLNTRTMLEEIYRGVEDGSPVELAVRTKNGE
                     IRQWLFYSSELGKLNQGRQARM"
BASE COUNT          292 a          265 c          357 g          278 t
ORIGIN      
        1 tcggcctggc ctcctctgtc gtctgagatc ggaaaccaag agtcgcccaa cgtcagcagt
       61 tgctccgatt tcgagttttt catttcgtcc tctgaaaaac ctgtctgctc gagaaatctc
      121 gggttccaag cgacaaacgt atgatggtcg aaatccagaa ttgctcttgg caaagcgaat
      181 tcattcaaga ccgcgcagaa atcctgcaag ttttggggtg agggatcgat acccaactaa
      241 atctctgccg ttggcgttaa gtcaagttgt gctttgaata gccagcatcc ggcgtggcgg
      301 ttgcaaacga tcacatagga tcagttagaa aggtgatcta tattgcgcac aattgcatgg
      361 atgtagagtg gaaattggcg aagagaaaca gcctgaattg ctgaggcgtg tctatggatg
      421 gaccctctga aagtcggtca ggatggggcg gagattcgaa tgccgaatgg atgaatcttg
      481 ccgtggaggc tgctgacatc ggaacttggg aatttgatct tgagcaaggg acgggtttca
      541 tctccgaacg atgcgctcaa ataatgggat atccgaaggt ttcatcaagc gggatgattc
      601 agttcagcga ctggctctcg atgatccact gggagtaccg gagacggatc aatcaagctt
      661 gcgatccgga gggagacggc gagctcaaga tgcgcctgca attgattgac acgggtggtg
      721 tgatcaggca tgtgctgatg cgagggcggg ccttttttgc agtcagtcat aggacgggaa
      781 gtagtccttt ccggaaggcg gtccgcttac taggcatcgt gcgtaatttg agcgatcgcc
      841 atatctatca gagagcgctc gccgaaagtg atcaacgatt gcgatgggcc ctgcaacacg
      901 ctcccattcc aatcatgatt cacttggatg atgggcagat aatcgaattg aatcgggcct
      961 ggcagcgcat caccggctat tcaatcgaag agattccgac gctggacgct tgggcagcgg
     1021 tttcatggga cccgttgaac acgcgaacga tgctggagga gatctaccgt ggcgttgaag
     1081 atgggagtcc ggtagaactc gccgttcgaa cgaagaacgg ggagatccgt cagtggttgt
     1141 tctattcgtc agagttggga aagctgaatc aaggacggca ggcccggatg at
//
LOCUS       DATMKH010000465         2623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2455335, whole genome shotgun sequence.
ACCESSION   DATMKH010000465 DATMKH010000000
VERSION     DATMKH010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2455335"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /locus_tag="VNV63_05620"
     CDS             complement(<1..722)
                     /locus_tag="VNV63_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62132.1"
                     /translation="MAAGEHRQAGRESKVCKGVEKGAKRIERTESIKIIVLKPDVTEK
                     EISHILEKLKEFGLKPHISRGEERVIIGAIGDERILQNQPLMVFPGVEKVMPILSPYK
                     LVSREFKESNSEIEVAKGIKVGGKRVRIFAGPCAIEKEDLLIRLAHQVKAAGATILRG
                     GAFKPRTSPYSFQGLEEKGLEFLLEAKKKTGLPTVTEVVDPRDVELVAKYADILQIGA
                     RNMQNFRLLTEVGAQHKPVLLKR"
     gene            complement(611..1726)
                     /gene="hisC"
                     /locus_tag="VNV63_05625"
     CDS             complement(611..1726)
                     /gene="hisC"
                     /locus_tag="VNV63_05625"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016483711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HXC62133.1"
                     /translation="MTIPVRKTIRGLSPYIPGKPVEELERELGIKNAIKLASNENPLG
                     PSKKAISILAKGFFKNLHRYPEGSGRLLREALAHKHKIAPDQVILGNGSDEIMDLAAK
                     TFLEPGDEVLIGDLTFSIYQISVTAHHGVGVVVPLKEGRYDLDGMARRLTPKTRLIFV
                     CNPNNPTGTMVTQREIVRFLDRLPAGVIVILDEAYAEYATSPDFPDSDGLVKQGRPLI
                     VLRTFSKVYGLAGLRIGYGISQQEIVEYLNRVRLPFNTNSLGQAAAIAALRDEAHLTK
                     SVRLNETGKVFLMNALDELEIRYFPTQANFIYLEVRLNGRSVYESLLREGVIVRHIKE
                     NWLRVSIGRPAENRRFVRALKKVLNESRERSPSRSLS"
     gene            complement(1734..>2623)
                     /gene="pheA"
                     /locus_tag="VNV63_05630"
     CDS             complement(1734..>2623)
                     /gene="pheA"
                     /locus_tag="VNV63_05630"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HXC62134.1"
                     /translation="PLDGVKAVFREIFSASLSLEGPIKVAYLGPKATFTHLACIQQFG
                     TSAAYVPVNSIKEVFNEVERGRADYGVVPIENSTEGVVNHTLDMFIDSPLKIWAEVLQ
                     EVSHHLLSKTGKLEAIKRIYSHQHAIAQCKTWLENNLPRTPISEVYSTAKAAEICVED
                     PTAAAIASELAARLYGLQIVKRRIEDNINNYTRFLVISDKLREKTGRDKTSIMFSIKD
                     RVGALYEMLKPFAQYGINLTKIESRPSKKKVWEYIFFVDLESHQEDKTVKSALEELKS
                     QCQFLKILGSYPANTKVQA"
BASE COUNT          561 a          772 c          649 g          641 t
ORIGIN      
        1 cgctttaaga ggaccggctt gtgctgcgcc cctacctcgg tcagcaacct gaaattctgc
       61 atgttccggg ccccgatctg cagtatgtcc gcatactttg ccaccagctc cacgtctctc
      121 ggatccacaa cctctgtgac ggtcggaaga ccggtctttt tcttggcttc caggaggaac
      181 tcaagcccct tctcctcaag accttgaaaa ctgtagggtg aggtcctggg cttgaacgcg
      241 ccgcctctca agatcgtggc gccggccgct ttaacctggt gggcgagccg aatcagaagg
      301 tcctccttct caatggcgca gggtccggca aaaatacgaa cccgcttgcc gccgaccttg
      361 attcccttgg ctacttcaat ctcgctgttc gattctttaa actcccggct cacgagcttg
      421 tacggggaca ggatcggcat gaccttctcg acgccgggga aaaccatcaa gggctgattc
      481 tggaggatgc gttcgtcccc gatcgctcca ataatgacac gctcctcgcc acgagagatg
      541 tgcggcttta aaccaaactc cttgagcttc tcaagaatat ggctgatctc tttctccgtt
      601 acatccggtt ttaagacaat gatcttgatg gactccgttc tctcgattcg tttagcacct
      661 ttttcaacgc ccttacaaac cttcgattct cggccggcct gccgatgctc acccgcagcc
      721 aattctcttt aatgtggcgt acaatcacgc cttctcggag aagcgactca tacacggatc
      781 ggccgttcag ccttacttcc agataaataa aattcgcctg ggtcggaaag taccggatct
      841 ccaactcatc cagcgcattc atcagaaaga ccttgccggt ctcgttgaga cgaacgcttt
      901 tcgtgagatg ggcttcatct ctcagcgccg cgatggcggc cgcctgaccc aagctgtttg
      961 tgttgaacgg cagccgtacg cgattcaggt actccacgat ctcctgctgc gagatcccgt
     1021 atccgatccg aagaccggcc agaccgtaaa ctttcgaaaa ggtccggagg acgatcaacg
     1081 gccttccttg cttcactaat ccatcgctgt ccggaaaatc cgggcttgtg gcgtactccg
     1141 catacgcctc atctaaaatc acgatcacgc cggccggcag gcggtccaaa aatcgcacaa
     1201 tctctctctg agtgaccatc gtcccggtgg ggttgttggg attgcagaca aagatcagcc
     1261 tggtcttggg cgtcagacgc cttgccatcc cgtccagatc ataacgtccc tcttttaacg
     1321 ggaccaccac gcccactccg tgatgggccg tgacggagat ctgataaatc gaaaaagtca
     1381 ggtcgcctat caagacctca tcgccgggct ccaggaaggt cttggccgca aggtccatga
     1441 tctcatccga gccgtttccc aaaattacct gatccggtgc gatcttgtgt ttatgggcca
     1501 gcgcctcgcg aagcaggcgt ccgctgcctt cggggtagcg gtgaaggttt ttaaaaaatc
     1561 cttttgccaa tatagaaatt gccttcttcg atgggccgag cggattctca ttggatgcca
     1621 gcttgatcgc attcttaatc ccgagttccc gctcaagttc ttccaccggc ttgccgggaa
     1681 tgtagggtga aagtccccgg attgttttcc tcaccggaat cgtcatggtc tttctatgcc
     1741 tgcactttcg tattggccgg ataagaaccc aaaattttta aaaactgaca ctgcgacttc
     1801 agctcctcca gagcggactt caccgtttta tcctcctgat ggctctccag atccacgaag
     1861 aagatatact cccagacctt tttcttggaa ggccgagact cgatcttggt gagattgatc
     1921 ccgtactggg caaagggctt gagcatctcg tagagcgccc ccacacggtc cttgatcgag
     1981 aacatgatgg aggtcttgtc cctccccgtt ttctcccgca acttgtccga gatcaccaag
     2041 aatcgggtgt agttgttgat gttgtcctcg atccgtcttt taacgatctg cagaccgtag
     2101 agccgggccg caagctcgct tgcaatcgca gccgcggtcg ggtcttccac gcagatctcg
     2161 gccgctttgg cggtgctgta tacctcgctg atcggcgtcc gcgggagatt attttcaagc
     2221 caggttttgc actgggcaat tgcgtgctgg tgtgaataga tccgcttgat cgcctcaagc
     2281 ttgccggtct tggagagaag atgatggctt acctcctgaa gaacctcagc ccagatcttt
     2341 aaaggcgaat cgatgaacat gtccagcgta tgattcacca cgccctcggt tgagttttca
     2401 atcggtacca cgccgtaatc cgcgcggccc cgctccacct cattaaaaac ctctttgatg
     2461 ctgttcacag ggacataagc cgcagaggtg ccaaactgct gtatgcaggc aagatgcgta
     2521 aaggttgcct tgggacccaa ataggccacc ttgatcggac cttccagaga aagcgaggcc
     2581 gagaatatct cccggaagac agcctttaca ccatcgagcg gaa
//
LOCUS       DATMKH010000466         1225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3724458, whole genome shotgun sequence.
ACCESSION   DATMKH010000466 DATMKH010000000
VERSION     DATMKH010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3724458"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="VNV63_05635"
     CDS             complement(<1..462)
                     /locus_tag="VNV63_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62135.1"
                     /translation="MIVLKADTPVFLRLSENLRTNEGVSEQGVRFQVTQEVRVGDSVL
                     IAKGAEAWGDAEITAKGPHLRSGKVVVRMDAVYAVTGDHVSLRGDISARGEPGCMAFD
                     CVLDLAVGIKGGDTSILKGTKVQAFVVKDISFDRVVTKRQFEEAERAEAMDR"
BASE COUNT          284 a          347 c          296 g          298 t
ORIGIN      
        1 gcggtccata gcttccgccc gttctgcctc ctcgaattgc cgtttcgtta cgaccctgtc
       61 aaacgagatg tccttgacga caaatgcttg aacctttgtt cctttgagaa ttgaagtatc
      121 accacccttg attcccacgg ctagatccag gacgcaatca aatgccatgc agcctggttc
      181 gccacgggct gaaatatcgc cacggaggga cacatgatca cctgtcaccg cataaacggc
      241 atccatccgt actaccacct tgccgctccg caggtggggc ccctttgccg tgatttctgc
      301 gtcgccccac gcttccgcac ctttcgcgat cagtacggag tcgcccaccc taacctcctg
      361 cgtgacctgg aaccggaccc cctgctccga tacgccctcg tttgtccgca ggttttcgga
      421 cagcctcaag aagaccggtg tatcggcttt tagtacgatc aagtcggtgt cggatgattg
      481 tggccatgtg gctggtgcac acattacggc aatactaatc aatacgaaga gtccgtttcc
      541 gaaaggaata gctttcattt catgccctcg gttgccgatc acttagtcgg aatttccgat
      601 taggacctct ggtccgaaaa tcgcggtctc cttaacgaat atcgtgctcc tgatctccct
      661 gatggccagc cctctggatt ttctcgaatg ttaacgaggg cgtttcctct tgggcttgag
      721 ggcctcaaga agtccattcc gaatcaaagc ctctttataa tcttcaaaac tcaaccgaga
      781 agaagccata acagcgtccc aaaaggacga tggcggatcg tagccgtgac cgtggatata
      841 gtggaggatt agcgagggag ctgcaaagac gaaattatcc gcagggacga acaggttcga
      901 taccccaagg tggatttcca gaacgtctgg attgtaacta tttttccttt tgaatatttc
      961 cgtctcgggt ccaagtattt caaaggtttc gaccccatcc gagatccttc gtcgtacaac
     1021 gaaacgactg tcgggcgaat attctgtgcg gcgcgacaaa tcgtgaggtc cgcaaaactc
     1081 acaggattgc ccaccaagga actcgcaggg ttgccagaga ttaacaaaaa gccgcagtag
     1141 gccgtggaac tgaacgattg acacggttcc cttgctgaac gggtgatccg gactaagcca
     1201 gcccacggct ttgattttct ctcct
//
LOCUS       DATMKH010000467         1466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3724977, whole genome shotgun sequence.
ACCESSION   DATMKH010000467 DATMKH010000000
VERSION     DATMKH010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3724977"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1398
                     /locus_tag="VNV63_05640"
     CDS             <1..1398
                     /locus_tag="VNV63_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012462437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXC62136.1"
                     /translation="KNPLNRFLIWIYQPFVNFVLRFRWLTILLSLLVLGLTWIPFSQL
                     GKEFMPPLNEGTLLYMPTAIPGMSMFEATKILQIQDRILRQFPEVESVFGKAGQADTS
                     TDPAPLSMFETVVQLKPPEQWPNGVTWDQLIARMNEASKTPGMAQIFWMPIQTRTEML
                     TTGFRSVLGIKVFGPDLKGIENVAVQIEQVLSDFPNTRSAFAERTTGGYFLDFSVNRD
                     AAARYGLQVQDVNQIVESAIGGNTVAMTVEGRERYPISVRYARDFRTDLDSLKRVLVP
                     TPSGAQVPISLLADISYRTGPPSIREENAQLVGYVFVDITSSDIEGYVRAAAKVIAQK
                     VHFPQGYYIQWAGQFEYLKAAEGRLAVLVPFTLLIIFFLIYLNTRSVIKTFIVLLSVP
                     FSLVGAFWMLFLLHYNMSVAVWVGLIALAGLDAETGVVMLLYLDHAWEKFKAQGRMNR
                     IEGPAKLKVPVNVKQ"
BASE COUNT          311 a          364 c          414 g          377 t
ORIGIN      
        1 aagaacccgc ttaaccgttt tctgatttgg atttaccaac cgtttgttaa tttcgttctt
       61 cgtttccgtt ggctgacgat cttactttcg ctcttggttc tcggcctgac ctggattccg
      121 tttagccaat tggggaagga gttcatgccg ccactcaatg aggggactct gctctacatg
      181 ccgacggcta tacctgggat gtcgatgttt gaagcgacga agattctcca gatccaggat
      241 cgcatcctgc ggcagtttcc ggaggttgaa agtgtctttg gcaaagcagg gcaggcggac
      301 acgtccaccg atcctgctcc gctctcgatg tttgaaacgg ttgtccagtt gaaacctccg
      361 gagcagtggc cgaatggcgt tacttgggac cagcttatcg cacggatgaa tgaggctagc
      421 aagactccgg gcatggctca gatcttctgg atgccgatcc aaacccgcac tgagatgctg
      481 acgaccggat tcaggtcagt gctgggtatc aaggtcttcg gccccgatct taagggaatc
      541 gaaaacgttg cggttcaaat cgaacaagtc ctctccgatt ttccgaatac ccgaagcgct
      601 tttgccgagc gaaccacggg ggggtatttc ctggatttca gtgtgaaccg ggatgcggcg
      661 gctcggtacg gccttcaggt ccaggatgtc aatcagatcg ttgagagtgc gatcggcggg
      721 aacacggtcg caatgaccgt agaaggtcgc gaacgctatc cgatcagtgt gcgctatgct
      781 cgcgattttc ggactgatct tgacagtctt aaacgtgttc ttgtgccgac gccgagcggc
      841 gcccaggtgc cgatttcctt gttggcagac atcagctatc gaaccgggcc tccatcgatt
      901 cgggaagaga atgcgcagct cgtgggttat gtgttcgttg atattacaag tagtgatatc
      961 gagggatacg tacgggccgc ggcaaaggtg atcgctcaga aagtccattt tccgcagggt
     1021 tattatattc agtgggcggg tcaatttgaa tacctgaagg cggccgaagg acgcctagct
     1081 gtcctggtgc cgtttaccct attgatcatt tttttcctga tctatctgaa cacgagatca
     1141 gtcatcaaga cctttatcgt ccttttgtcg gtccccttct cgttggtggg ggcattctgg
     1201 atgctgttcc tgctccatta caacatgagt gtcgcggttt gggttggcct gatcgctctt
     1261 gccggccttg atgccgagac cggggtggtc atgctcctct atctggacca cgcctgggaa
     1321 aagttcaaag cgcaaggacg catgaacagg atcgaaggac ccgcgaagct aaaagttccg
     1381 gtcaacgtta agcagtaaga tgtcctggtt ggaatcagtg gagaattccc acttttcgcg
     1441 actgtggctg aagagaacaa tccaga
//
LOCUS       DATMKH010000468         1381 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1183827, whole genome shotgun sequence.
ACCESSION   DATMKH010000468 DATMKH010000000
VERSION     DATMKH010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1183827"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..791)
                     /locus_tag="VNV63_05645"
     CDS             complement(<1..791)
                     /locus_tag="VNV63_05645"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HXC62137.1"
                     /translation="MAFFSKIIRFIYRNRSLRFTKEGLSFILFTFAVGIAAVNTGNNL
                     LYLILAMMLSLIIVSGVLSEQCLRHLTVTRHFPSQLFARSLTIFHVRVANHHRVFPSF
                     SIQVKEEEGTSSQSGEAYFFKIPAGATQTSGCSITPAHRGRYIFQNVAFRTRFPFGLF
                     QKSLIRLVPDEVLVYPTIYSVQTLIEEDLLTIGTAQELNRRGTGATLYNLRDYQEGDD
                     ARNIHWKTSARQARLFSKQYEQEEEKKIRLTLDQELSGDQQSGKH"
     gene            complement(855..>1381)
                     /locus_tag="VNV63_05650"
     CDS             complement(855..>1381)
                     /locus_tag="VNV63_05650"
                     /inference="COORDINATES: protein motif:HMM:NF036831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HXC62138.1"
                     /translation="FMVVATQNPMEYQGTYPLPESQLDRFMMRIRIGYPSEADEKKIL
                     QAQQSFHPVEGLKPVLTGAEVVKLQDQVVHVRVDESLVDYLLAIVTVTRQSDRLGLGA
                     STRAAMSLHKAAQALAFVEGREYCLPDDFKYLAPFVLSHRVIINARLDAHGYRSQDAE
                     EIITDLVDQIPVPL"
BASE COUNT          343 a          361 c          365 g          312 t
ORIGIN      
        1 aaatgtttcc cgctctgctg atcgcccgat agttcctggt ccagtgtgag acggatcttt
       61 ttttcctctt cctgttcata ctgtttgctg aacagtcgag cctgacgggc cgaggtcttc
      121 caatgaatgt tccttgcatc atcgccctct tgataatccc ggagattgta aagggttgcc
      181 cctgttcccc gccgattcaa ttcctgtgca gtgccgattg tcagcaggtc ttcctcgatg
      241 agtgtctgga cagaataaat ggtaggatag accagcactt cgtccggaac caggcggatt
      301 aaactttttt gaaacagtcc gaatggaaac cgggtgcgga aagccacatt ttggaagatg
      361 tatcggccac gatgcgcagg cgtgattgag caaccgctgg tctgagtagc gccagccgga
      421 atcttaaaga aataagcctc gccgctttgt gagctcgtcc cttcttcctc tttgacctgg
      481 atcgagaagg atggaaacac acgatggtga ttcgccaccc tgacgtggaa tatagtcagt
      541 gaacgggcaa aaagctgaga cgggaaatgc cgggtgacgg tgaggtgcct aaggcactgc
      601 tccgagagaa caccggagac gataatcaga ctcagcatca tggccagaat aagatagagc
      661 aggttgttac cggtgttgac cgccgcaata cctaccgcaa aggtgaacag gatgaagctt
      721 aaaccttcct tggtgaatcg aagagagcgg ttgcgataga taaaccggat gattttagaa
      781 aaaaaggcca tcgtatttcc cgtaggggcc cgtatttccc gcaggagcca gtatctcccg
      841 ttggagcccg gcgactaaag cgggacaggt atctgatcca caagatcagt gataatctct
      901 tcggcatcct gactgcggta cccatgggca tcgaggcggg cgttgatgat cacgcggtga
      961 gagagcacaa acggagccag atacttaaaa tcatccggaa gacagtactc ccttccctcg
     1021 acaaaggcaa gcgcctgcgc cgccttgtgc agagacatcg cggcacgcgt gctggctccg
     1081 agacccaacc gatccgattg acgagtgacg gttacaatcg cgagtaaata atccaccagg
     1141 ctctcatcca cccggacatg gaccacctgg tcttgaagct tgaccacctc tgcaccggtc
     1201 aagaccggtt ttagcccttc gaccggatgg aaagactgct gggcctgaag gatcttcttt
     1261 tcatctgcct ctgacggata tccaatacga atcctcatca taaatcggtc cagttgagac
     1321 tccggcaagg ggtaagtccc ctgatactcc atcggatttt gagtggcaac taccatgaac
     1381 g
//
LOCUS       DATMKH010000469         1197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3725094, whole genome shotgun sequence.
ACCESSION   DATMKH010000469 DATMKH010000000
VERSION     DATMKH010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1197
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3725094"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1197)
                     /locus_tag="VNV63_05655"
     CDS             complement(<1..>1197)
                     /locus_tag="VNV63_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HXC62139.1"
                     /translation="QGSKIAFDHTGMIFETSVSLEAVPGEAYLGLGEKTGPINKRGLS
                     FVFWNTDIVPYHIDSDPLYLSIPFFIAVRPDYVWGCFLNESWRTEVDLAKSDPSQIRW
                     SSSGSEMDLYLFSGPTLRDVVQQYTQLTGRTPLPPLWSLGAQQNRWGYEKAEDVLRVI
                     QGYRNHGLPLDAIYMDLDHMVANKAWTWNREVFSDPERLVKQAAAQGVKLIPIVNPGI
                     KVEAGYAVYDEAVGKNYLVSTDRGVPLRGEVFPNPSVFPDLLREEVQTWWGDLHKNFI
                     TAGIAGFWNDMNEPSNFSISGSKHSAIKTEDDGLGKIEGRTLPYDARHGKKRHLEVHN
                     LYGLCMCKSSFEAVQRFAPDKRPFVLTRAGFAGSQRYGPVWTGDVSSSWSHLSASISM
                     LVGLGLS"
BASE COUNT          293 a          318 c          305 g          281 t
ORIGIN      
        1 agaaagcccg agaccgacta acatcgaaat agaggcgcta agatgggacc aggagctgga
       61 gacatctcca gtccagacag gcccataacg ctggctgccg gcaaaaccgg ctcgagtgag
      121 tacaaacggc cgtttgtcag gtgcaaagcg ctgcaccgcc tcgaaggaag atttacacat
      181 gcaaagtcca tacaggttgt gcacttccag atgcctcttt ttcccgtggc gggcgtcata
      241 aggcagcgtc ctgccttcaa ttttccccaa tccgtcatct tcggttttga tggcgctatg
      301 tttgcttccc gaaatggaaa aattgctcgg ttcgttcatg tcattccaga agccggcaat
      361 tcctgcggta ataaaatttt tgtgaagatc gccccaccag gtttgcacct cctctcgcaa
      421 taaatccggg aaaacggatg ggttaggaaa aacctctccg cgcagtggta cccctcgatc
      481 agtcgatacg aggtagttct tgccaaccgc ctcatcgtac accgcataac cggcctcgac
      541 cttgataccc ggattcacga taggaatcag tttaacaccc tgagcagctg cctgtttaac
      601 gagtctctcg ggatcggaga aaacctcacg attccaagtc catgctttat tggccaccat
      661 gtgatccaga tccatgtaga tggcgtcgag aggtaaaccg tggttccgat aaccttggat
      721 gactctaagg acgtcctccg ctttctcgta gccccaccgg ttttgttgag caccaagtga
      781 ccacagtggc ggaagtggag ttctgccggt cagttgggtg tactgttgca ccacatcgcg
      841 cagggtcggc ccgctgaaaa ggtaaaggtc catttccgag ccactggaag accagcggat
      901 ttggcttgga tccgattttg ctagatcgac ttcggttcgc cagctttcat tcagaaaaca
      961 gccccataca tagtccggcc tgaccgcaat gaaaaagggg atcgaaagat ataaaggatc
     1021 ggaatcgata tgatagggga cgatgtctgt gttccaaaag acgaatgaga gtccacgttt
     1081 attaatcggg cccgtctttt caccgagccc aaggtaagcc tctccgggaa ccgcttcgag
     1141 actgacgctg gtttcgaaga tcattccggt gtgatcaaag gcgattttgc tcccttg
//
LOCUS       DATMKH010000470         1088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3725318, whole genome shotgun sequence.
ACCESSION   DATMKH010000470 DATMKH010000000
VERSION     DATMKH010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3725318"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="VNV63_05660"
     CDS             <1..706
                     /locus_tag="VNV63_05660"
                     /inference="COORDINATES: protein motif:HMM:NF028479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HXC62140.1"
                     /translation="VELNPYDPERHVWVVDRDGDQILKFTNEGKKLVLRLGEKGVPGS
                     DHSHFNWPASLLFMPDGSFYVADGYHNTRVVKFDKSGTYLTEWGGKGNGPGQFNLIHD
                     VAIDAQHRIYVADRGNNRVQIFREDGTFLDQWPNIHNPSHFWITKDGYLWLVSGDGNR
                     LAEFDLNGKLITYWGMHGRFAGGFDDPHTIDVDSLGNLYVAEVWNNRVEKFVPREDAD
                     KSRLIGQKFILKGGLN"
     gene            complement(836..>1088)
                     /locus_tag="VNV63_05665"
     CDS             complement(836..>1088)
                     /locus_tag="VNV63_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62141.1"
                     /translation="GRLRSALDQVGVASGNLATILLTHLHQDHSAGLTDGKGNAIFLN
                     AEIVMERAEHAFWTEDSNFKDSPDNFKRYVSLIQTSMVS"
BASE COUNT          253 a          269 c          292 g          274 t
ORIGIN      
        1 tgtcgaatta aatccgtatg accctgagag acatgtgtgg gtggtagatc gcgatggcga
       61 ccaaatcctc aaattcacca atgagggcaa gaaactcgtg cttcgccttg gcgaaaaagg
      121 agtaccgggc tcagatcaca gccatttcaa ttggccggca tctttgctgt tcatgcctga
      181 cgggtccttc tatgtggcgg atggttacca caacacccgt gtcgtgaaat ttgataaaag
      241 tggaacgtac ctcacggaat ggggtggtaa aggcaatgga cctgggcaat ttaatctgat
      301 tcacgatgtc gccatcgatg cgcaacaccg tatctacgtt gccgaccgcg gcaacaatag
      361 ggtccagatc ttccgcgaag atgggacgtt tctcgaccaa tggccgaaca ttcataatcc
      421 gagccatttt tggatcacca aagacggata tctgtggctc gtctccgggg atggtaatcg
      481 cttggccgag ttcgatctca acggcaagct cattacttac tggggcatgc atggcaggtt
      541 tgctgggggg ttcgatgatc cgcatacaat tgatgtcgac tctttgggca atctttatgt
      601 cgcggaagtc tggaacaacc gcgtcgaaaa attcgttccc cgcgaagatg ccgataaatc
      661 ccgtctcatc ggacagaagt tcatccttaa aggtgggctt aattagaatg gcgggcttgc
      721 cccggtggcg gtttcccgcc tagccggatt cccggcgtgt gaccgtggag cggtacagac
      781 catattttgg gaagaacctc gctctctctc cgtcgaataa ccgggtacgg tccttctatg
      841 aaaccataga cgtttgtatc aacgagacat atcgtttgaa attgtccgga gaatctttga
      901 aattgctatc ttcggtccag aacgcgtgct ccgctctctc cattacgatc tccgcattga
      961 gaaagattgc attgcctttg ccatctgtga ggcccgccga gtgatcttga tggagatgtg
     1021 tcaggagtat cgtggctaag tttccagatg cgactcctac ctgatcgagg gctgagcgaa
     1081 ggcgtccc
//
LOCUS       DATMKH010000471         1020 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_861658,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000471 DATMKH010000000
VERSION     DATMKH010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_861658"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..915)
                     /locus_tag="VNV63_05670"
     CDS             complement(<1..915)
                     /locus_tag="VNV63_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HXC62142.1"
                     /translation="MLALVAIAIVSATIFYSQYLDRKIAEDERSKVQLWVAATKAIIN
                     NPTMDLTLPNLIRNGQQSIPIIETNEQDSIMEFINLDTAQASRDPNYLYNQLKKFKAQ
                     DSPVEVEFSKKPYIANRYYYGHTSLLDEVRYYPLVQLFIVALFIFFILYTITIRNKAT
                     QNQLWAGMAKETAHQLGTPVSSLEGWVEMLKESTLDPRTVQEIQKDVNRLKLVSDRFG
                     KIGSRPNLEECDLVAQIRTMMEYIRKRATGKVQFTLQPDEGEVPARISGPLFDWVIEN
                     LLKNALDAMEGKGSITINIQDLEKEILID"
BASE COUNT          202 a          235 c          309 g          274 t
ORIGIN      
        1 gtcgatgagg atctcttttt ccaaatcctg gatattgatc gtgatggagc ctttgccttc
       61 catggcgtcg agggcgttct tgagcaggtt ctcgatcacc cagtcgaaca gggggccgga
      121 gatgcgggcg gggacttctc cctcgtcggg ctgcagggtg aactggactt tgccggtcgc
      181 gcgtttgcga atatattcca tcatggtgcg gatctgtgcg acgaggtcac attcttcgag
      241 attgggccgg ctgccgatct ttccaaagcg gtcggatacg agcttgaggc ggttgacgtc
      301 cttctggatc tcctgtacgg tccggggatc aagcgtcgac tcctttagca tctcgaccca
      361 gccttcgagc gaggagacgg gtgttccgag ctggtgggcg gtctccttcg ccatccctgc
      421 ccagagctgg ttctgggtag ccttgttgcg gatggtgatc gtatacaaaa taaaaaagat
      481 gaagagggca acgatgaaga gctggacaag cggataatat ctcacttcat ccagcaggct
      541 ggtatggccg taatagtatc ggttggcgat ataaggtttc ttactaaact cgacttcgac
      601 cggcgagtcc tgggccttga acttcttcag ctggttgtac aggtagttcg ggtcccggct
      661 ggcctgtgcg gtgtccaggt tgataaattc catgatgctg tcctgttcgt tggtttcgat
      721 gatggggatg gactgctggc cattgcggat caggttcggg agggtgaggt ccattgtggg
      781 gttgttgatg atggccttgg tggcggctac ccacagctgc actttcgatc gttcgtcttc
      841 tgcgatcttc cggtcgaggt attgggaata gaagatcgtc gcgctcacga tggcgatggc
      901 taccagggcc agcaacgatc tccaattcag taatgatcga atcataaacg aatttataaa
      961 aagcgcagca ctaataggcg attgccttat ttttgggcat tatatctatt attagtgacg
//
LOCUS       DATMKH010000472         1321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4056246, whole genome shotgun sequence.
ACCESSION   DATMKH010000472 DATMKH010000000
VERSION     DATMKH010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1321
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4056246"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VNV63_05675"
     CDS             complement(<1..435)
                     /locus_tag="VNV63_05675"
                     /inference="COORDINATES: protein motif:HMM:NF028434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HXC62143.1"
                     /translation="MATKGKVLVLVSSGRGLPLKDGGCYKGAGYYLNELTVPVRALMK
                     EGYEITFANPKGNTPQVDVHSEVPDFFGGDAAKLEDYMLFRDGLKGLRDPSRISDVIA
                     SGLDQYDAVFVPGGHGPMIDLLDDPDAGIVMRHFHETSKPTAV"
     gene            complement(581..976)
                     /locus_tag="VNV63_05680"
     CDS             complement(581..976)
                     /locus_tag="VNV63_05680"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008141645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HXC62144.1"
                     /translation="MKREIIRVEPLATYLEKYKAPASTVTKHGDTIYVSGSPPFDPKT
                     GEIFRGPIERQTEIVLDQLKLCVETAGSSLENILKLNVYVTSVDMFPAVNEVYRRYFP
                     ENPPARIFINVPAWNGGFDIEVHCIAAAL"
     gene            complement(1135..>1321)
                     /locus_tag="VNV63_05685"
     CDS             complement(1135..>1321)
                     /locus_tag="VNV63_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXC62145.1"
                     /translation="HKKPGAPDSPSTIQDTKRYLEDFDRMQKTATSDQELFDQMTELY
                     PHWVANQSWLMFGFPQP"
BASE COUNT          298 a          355 c          350 g          318 t
ORIGIN      
        1 cactgccgta ggcttggatg tttcgtgaaa gtgcctcatc acaatgccgg cgtcggggtc
       61 atcgagaaga tcgatcatcg gaccatgccc tccggggacg aatacggcgt catattgatc
      121 caggcccgat gcgatgacat ccgagatgcg gcttggatct ctgagcccct tcaggccgtc
      181 tcggaatagc atatagtcct caagcttggc cgcgtccccg ccgaagaaat cggggacctc
      241 tgaatgcaca tccacctggg gcgtgttgcc tttgggattg gcgaaagtga tttcgtagcc
      301 ctccttcatg agggcgcgaa ccggaaccgt cagttcgttg agatagtagc cagcaccctt
      361 gtaacatccg ccatctttca agggcaagcc gcgaccgctc gagactagta ccaggacttt
      421 tccttttgtt gccatcgttg ttctcctttt tgaaatcgaa tctgtttcct gagatcttaa
      481 ccttaaaact ggggacagac gggacgttca ctaatttcca ttgtcgaaaa atggaaaacg
      541 tcccgtctgt ccccggtttc ccgtcccaca atcgacagat ctaaagagcc gcggcgatac
      601 agtgcacctc gatgtcgaag ccgccattcc aagcaggaac gttgataaag attcgagcag
      661 gaggattctc cgggaagtaa cgcctgtaga cctcgttaac agcaggaaac atatccactg
      721 aagtcacgta gacattcagt ttcaggatat tttccaagga tgaacccgcc gtttcgacac
      781 agagcttaag ctggtccaaa acgatctcgg tctggcgctc aataggccct cggaaaatct
      841 ctccggtttt cggatcgaac ggcggagagc ctgagacgta aatagtgtcg ccgtgtttag
      901 tcaccgttga agcgggcgcc ttgtactttt cgaggtaggt cgccagcggc tcgacccgaa
      961 tgatttctcg tttcatagat ttcctccgtt ttgtagacac ggtttccagc aaccaagagc
     1021 cgcgagattt cagggtagga gtaaagcgag actctggaac agctgtctgc ctgaggtcat
     1081 cggctaggcc tccacccgaa ttgtaactct tctcgcatca gcgctagatg tggcttatgg
     1141 ctgcgggaat ccgaacatca accacgactg attggcgacc caatgcgggt acagctccgt
     1201 catctgatcg aacaactcct gatcggacgt cgctgttttc tgcatccgat cgaagtcctc
     1261 gaggtaacgc ttggtgtctt ggatcgtcga gggagagtcg ggcgcgccag gctttttgtg
     1321 a
//
LOCUS       DATMKH010000473         1006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3727476, whole genome shotgun sequence.
ACCESSION   DATMKH010000473 DATMKH010000000
VERSION     DATMKH010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3727476"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..>1006
                     /locus_tag="VNV63_05690"
     CDS             164..>1006
                     /locus_tag="VNV63_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-54 factor"
                     /protein_id="HXC62146.1"
                     /translation="MALLQPKLSLKVAQRQILTPGLMQMVSVLALNKLELKEMIQSEI
                     AENPILEELEENVPLLDEVAGREEMLDRPVEQQQAEAAREKTDPFDEIDFGSYFQDYL
                     DPGFRTPNSFEITEKPSIENFLSQPSTLTDHLFWQLGSLSLTPVIRDAAEYVIGNLNE
                     DGYLIATDDELLEGYLREQLGPEKDSGQRNQPQGQIQLAEVPAVMAERARGHLAAALD
                     VVQHLDPVGVATRSLSECLLVQIGAQQHEFEMIYERQKRSAYINGTSHRDAAEVLLSN
                     DYADE"
BASE COUNT          249 a          239 c          301 g          217 t
ORIGIN      
        1 gagggattct ccctgggtta agccgattgc aaggtttggt gagatcggca ggttacgaaa
       61 ggcggcggga ccttaagggt tttcaggaag gtcttctaag gcgttcccac ctggatgtac
      121 actgggaggc accactctgc atttttcttg aagatgggat gcaatggctc ttctccaacc
      181 caagctgagc ttgaaagtag ctcaacggca gatattgacg cccggcctga tgcagatggt
      241 cagcgtttta gcgctgaata aactcgagct gaaagagatg attcaatcgg aaattgccga
      301 gaatcccatc ctggaagagc ttgaagagaa tgtgcctctt ctcgacgagg tggccggcag
      361 ggaagagatg ctggacaggc cggtcgaaca gcagcaggcg gaagctgctc gcgagaagac
      421 cgatccattt gatgaaatcg actttggctc ttatttccag gattatcttg atccgggctt
      481 ccgtacgccg aacagcttcg agatcacgga aaaaccgtcg attgaaaatt tcctttccca
      541 gcccagcact ctcacagacc acctgttctg gcagctcggt tcactgagcc tgactcccgt
      601 catccgggat gccgcggaat atgttatcgg caatctgaat gaagacggat atttaattgc
      661 cacggacgac gaattgctgg aagggtacct gcgagaacaa ttagggccgg aaaaggacag
      721 cgggcagaga aatcagccgc agggtcagat tcagctggcc gaagtacccg ccgttatggc
      781 tgagagagcc agaggtcatc tggcggctgc gctcgatgtg gtgcagcatc ttgatccggt
      841 gggggtggca acacgcagtt tgagcgaatg cctgctggta cagattgggg ctcagcagca
      901 cgaattcgag atgatctatg agcggcagaa gcgttccgcg tatatcaacg gaacgagcca
      961 tagagacgcg gctgaggttc tcttaagcaa cgactatgcg gatgag
//
LOCUS       DATMKH010000474         1042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_862787,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000474 DATMKH010000000
VERSION     DATMKH010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_862787"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            224..877
                     /locus_tag="VNV63_05695"
     CDS             224..877
                     /locus_tag="VNV63_05695"
                     /inference="COORDINATES: protein motif:HMM:NF025854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HXC62147.1"
                     /translation="MHEVNNRLAALTNLIFLARTLPECALQSIEYLDEADSQLRSLGE
                     VTSRSLSFIRLDMEAKEIDLVDLATAALRLHHAQISQKRIKVETRSSESVLARVKKGE
                     ILQVITNLLLNSIEALPHSGNLHVRVVTRRSNAIITIADNGAGIPGSMRKTLFDSFKS
                     NKSEGNGLGLWIVREIVQGHGGTIQYRSSGVVGRSGTVFRVSLPIQSLACAPSGEGS"
     gene            complement(838..>1042)
                     /locus_tag="VNV63_05700"
     CDS             complement(838..>1042)
                     /locus_tag="VNV63_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013573024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXC62148.1"
                     /translation="FEAFFTTRSTVGTGIGLFVAKQFIEGHGGRIEIESRQSAEDHGT
                     TVRVLLPLSTTYDPSPEGAHAKD"
BASE COUNT          236 a          287 c          278 g          241 t
ORIGIN      
        1 tggttttccg ctggttttcc tgccctggtt tttcctgggg tatacagctc aaagaccctc
       61 cgtgggccaa cctttatttc tccaacctcc tcagataggc atggattggc cagtcgctac
      121 gctaggctcc tctagggacg aaggtcaaaa gctcaaagcc tgcgaagaca atctgcgaag
      181 gtatcaccaa ctcgccctgg caggtagact ggtcggcgcg accatgcatg aggtcaataa
      241 tcgcctggcg gccctcacca accttatctt tctcgcgaga acccttccag agtgcgcact
      301 ccagagcatt gaatatctgg atgaggcgga tagccagttg cgcagcctcg gagaggttac
      361 cagtagaagt ctctccttca ttcgtctgga tatggaagcg aaagagatcg atttggtcga
      421 tttagctacg gccgcacttc gcctgcacca tgcgcagatt tcccaaaaaa gaatcaaagt
      481 cgagacgcgg tcttccgaat ctgtattggc gcgtgtaaag aagggtgaaa tcctccaggt
      541 gattacgaac ctcctgctca actccatcga ggccttgcct cattccggga atcttcatgt
      601 gcgtgtcgtc acgcgtcggt ccaatgccat catcacgatt gcagacaacg gcgctggaat
      661 cccggggtcg atgcggaaaa cgctcttcga ttccttcaag agcaacaaat ctgagggaaa
      721 cggactcggc ctctggatcg taagagagat cgttcaaggg catggcggga caattcaata
      781 caggagcagc ggtgttgtcg gcagaagtgg aacggttttc cgcgtttctt tgcccattca
      841 atccttggcg tgcgcgccct ccggggaggg atcataggtc gtggatagcg gcagaaggac
      901 gcgcacggtt gtaccgtgat cctccgcgct ctgcctgctt tcaatctcaa tgcggcctcc
      961 gtggccctcg ataaactgtt tcgcaacaaa caaaccgata cctgttccca cagtgctgcg
     1021 ggtcgtgaag aaggcttcaa at
//
LOCUS       DATMKH010000475         1195 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_222720,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000475 DATMKH010000000
VERSION     DATMKH010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222720"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(199..1035)
                     /locus_tag="VNV63_05705"
     CDS             complement(199..1035)
                     /locus_tag="VNV63_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62149.1"
                     /translation="MFIRSCVLYLISLSLSHPGIAQWNPPESLRGIPVRNDCKKTQGG
                     IGRSFQPSLFNPHGIIFLCPDRAEAIDQRHPGASFFFRVHEYGHLALGTRDEAAADAW
                     AAEQLSHSDAGRVILRAVLAHFADIGEKFAPYYGTGFYRGLNVATTAGIDRKEWPETL
                     VAYQRKWEDRLKRNGSISFQSKEQSVFDGLVVIDGETLGFFDTMYADRSLPLPALNQG
                     KHELSLVNVWSYQIGQNHKLLALVRGMNTTASFTISNTGDLVGYISGSESDLSVVVRG
                     RQ"
BASE COUNT          290 a          308 c          305 g          292 t
ORIGIN      
        1 tctcggtagg agcgatatct tcagcgaacc tagggctcct cagcagcata gcaatatcga
       61 agcccaaaca tgcacagctg gttcttagag agatgccgct cctacggagc tgggcttggt
      121 tttggactcg atagctataa aggttccgct cctaacggag ctggacccag ctatccagag
      181 attgcaccga gcgcgtccct actgccgtcc tctaactacc accgacagat ctgattcact
      241 tcccgaaata taaccgacca gatctccagt gttagatatt gtaaatgaag cagttgtgtt
      301 catccctcta accaaggcca ggagtttgtg attttggccg atctgatagc tccacacatt
      361 aaccaaggaa agctcgtgtt ttccttggtt tagcgcgggt aatggcaggg atcgatcggc
      421 atacattgtg tcgaagaaac cgagcgtttc accatcgatc accactaacc catcgaacac
      481 cgattgctct ttactttgaa aagagatcga gccgtttcgc ttgaggcgat cctcccactt
      541 ccgttgataa gcgactaagg tctccggcca ttcttttcga tcgatcccgg cggtggtggc
      601 cacattcagt ccgcgataga atccggttcc gtaataagga gcaaattttt ctccgatatc
      661 ggcgaaatgt gccagaactg cgcggagaat aactcgcccc gcatcgctat ggctaagctg
      721 ttctgctgcc cacgcatccg ctgccgcttc atcccgggtg ccaagggcaa gatgaccata
      781 ttcgtgcacg cgaaagaaga aggaagcgcc gggatgtcgc tgatcaatgg cttcggctcg
      841 gtcaggacaa agaaagataa ttccatgcgg gttaaacagg gatggctgga atgacctgcc
      901 gattccgccc tgcgtcttct tacaatcatt gcggacggga atacctcgaa gggattccgg
      961 cggattccat tgagcaattc cagggtggct aagagacagc gagataagat aaagaacaca
     1021 agagcgaatg aacattttcg aggagcccgc caagacagaa tcttttcaca ggaatgccgc
     1081 aaaggccaca aagagggatt ttctgtctcg gatcttcacg gcctttgcgg cgttgttgtg
     1141 aatttttttg cttggccttt gtttgcttgt gtgagattcc ggttgtcctg tcgcg
//
LOCUS       DATMKH010000476         1430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_863554,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000476 DATMKH010000000
VERSION     DATMKH010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1430
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_863554"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="VNV63_05710"
     CDS             complement(<1..526)
                     /locus_tag="VNV63_05710"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HXC62150.1"
                     /translation="MYLTQQSKLLFLITLSLWTVFLWPGRAEIQSRTSLRRLYVANEG
                     SDSVSVIDPADLSLVARVPTGVHPHNVSVDPQGRYFYVTVLFTKESDDLVQIFDVKTN
                     ALVTAVHVEHQPAHVVPDPTGKRLYVSSEFGNSVSIIPVPEFKKVETVKLKGRGPHGQ
                     VITPDGRVLLVPNGR"
     gene            complement(542..>1430)
                     /locus_tag="VNV63_05715"
     CDS             complement(542..>1430)
                     /locus_tag="VNV63_05715"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HXC62151.1"
                     /translation="FEDRESNVHLVATTPAYPSVANLRLTEGRFLREGDLEDRRKVCI
                     LGWGKKEELFPHDDPLGKVINIEGQLYQVIGVLENRDLPKGKNPVVQARDINNDIYIP
                     LVAVFSSLSSETGVNEISIRVKDASQVLMAANMAKSILDRIHNHVNDFEIIVPQELLK
                     QSQRTQRIFNVVMGSIAGISLLVGGIGIMNIMLAIITERTKEIGTRRALGANKMDILR
                     QFLVETLVLTVAGGVIGILLGSAGAKIINYYVEWRTVVSINAIFVSFFVSVLVGVIFG
                     MYPAYKAANMNPIEALRYE"
BASE COUNT          349 a          381 c          359 g          341 t
ORIGIN      
        1 agcgaccgtt ggggaccaat aggacccggc catccggggt gatcacctgt ccgtggggac
       61 ctcttccttt cagcttcaca gtctcgacct tcttaaactc cggaactggg atgatagaaa
      121 cgctgttccc gaattcgctg gaaacataga gacgctttcc agtgggatcc gggacaacgt
      181 gggccggctg atgctccacg tgtacagcgg tcaccagggc attggtcttt acatcgaaaa
      241 tctgaaccag atcatccgac tctttcgtga aaagaacggt cacatagaaa taccttcctt
      301 gagggtccac gctgacatta tgagggtgca cgccggttgg aacacgggca acaaggctta
      361 gatcggcagg atcaatgacc gagacgctgt ccgagccttc gtttgcaacg tataaacgcc
      421 ggagagaggt gcgggactgg atctctgccc tgccaggcca aagaaagacg gtccacaggc
      481 tgagtgtgat taaaaaaaga agtttacttt gttgcgtgag atacacagga aaacacgtct
      541 tttactcgta ccgcagcgcc tcgatagggt tcatgttggc ggccttgtag gccgggtaca
      601 tgccgaaaat aacgccgaca agaactgaga cgaagaacga tacaaaaatg gcattgattg
      661 aaacgacggt cctccactct acataataat tgatgatctt ggccccggct gagcccagta
      721 aaattccaat aaccccgccg gcgactgtca agacaagcgt ctccaccaag aactgtctta
      781 agatgtccat cttattggcc cccaacgccc tccgggtccc gatctctttg gtccgttcgg
      841 taatgatggc cagcataatg ttcatgatcc cgatgccgcc gactaacaga gagattccgg
      901 cgatgcttcc catcacgaca ttgaaaatcc gctgcgttct ctggctttgt tttaagagct
      961 cctgcgggac gatgatctca aaatcattta catgattatg aattcggtcc aggatggact
     1021 tggccatgtt ggcggccatt aggacttggc tggcatcctt cacacggatc gagatttcgt
     1081 tcacaccggt ctccgaagat agagaagaga aaaccgcaac caaggggata tagatgtcat
     1141 tattaatatc ccgggcctgc accaccgggt tttttccctt ggggaggtcc cggttctcca
     1201 ggacgccaat gacttgatac aactgcccct cgatgttaat caccttcccg agcggatcgt
     1261 catggggaaa gagctcttcc ttttttcccc atcctaatat gcataccttt ctgcggtcct
     1321 ccaggtctcc ttctcttaaa aatcttcctt cggtcagccg gaggtttgca accgatggat
     1381 aggcgggtgt ggtcgccacc aggtggacat tgctttcccg atcttcaaag
//
LOCUS       DATMKH010000477         1269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1187234, whole genome shotgun sequence.
ACCESSION   DATMKH010000477 DATMKH010000000
VERSION     DATMKH010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1187234"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..921
                     /locus_tag="VNV63_05720"
     CDS             <1..921
                     /locus_tag="VNV63_05720"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXC62152.1"
                     /translation="APGLIYYMTIWFPKRELSKVISYYYLGLPLSSIIAGPLSGLILD
                     HMNGYLGLKSWQWLFILEGIPALIIAVVCLVYLTNTPKEAKWLTDEERDAYDRMMEQE
                     RKEVSSHGHADLKASLLSPQVWILGIAYCATVFGLTGLVFWMPQIIKQFKVTDTIVGL
                     LSTIPYLIGAIIMCWWAARSDSKKERFWHFIIPSLVFSLGFVLTAVSSSPWASLLGMV
                     ISTAAFLSAVPVYYTLPASFLVGAASAGGMAVCNAVGNIGGFFGPWILGYIRQRSGAP
                     AALYVCALVVLIAPALVSYLRFTNKQMPWK"
BASE COUNT          272 a          338 c          318 g          341 t
ORIGIN      
        1 gctcccggac tgatctatta catgacaatc tggttcccaa aacgggaact ctcgaaggtc
       61 atctcttatt attatcttgg actgccgctc tcctcgatta ttgccggacc gctttcgggc
      121 ctgatcctgg atcacatgaa tggctatctt ggtctgaaga gttggcaatg gcttttcatt
      181 ctcgaaggaa ttccggcgct cattatcgcc gtcgtctgtc tcgtgtatct gaccaatacg
      241 ccaaaggaag ccaagtggct gacggatgaa gaacgggatg cctacgatcg gatgatggag
      301 caggagcgca aagaggtctc ttcacacggg cacgcggacc tcaaagcttc tctgcttagt
      361 ccacaagtct ggatcctagg tatcgcctat tgcgcaaccg tgtttggcct caccggtctt
      421 gttttctgga tgccgcaaat cattaagcaa ttcaaagtta ccgatacgat cgtgggcttg
      481 ctgagcacga ttccttatct tatcggtgct atcatcatgt gctggtgggc ggcaaggtcg
      541 gactccaaaa aggaacggtt ctggcacttc attattccgt cgctggtctt ttcacttgga
      601 ttcgtcttga ccgcggtctc ctcgtcgcct tgggcatctc ttctcggaat ggtcatcagc
      661 accgcggcat ttctatctgc ggttccagtg tattacacct tgcctgcctc atttctcgtc
      721 ggcgcagcgt ctgccggagg aatggcagtc tgcaacgcag tggggaatat cggtggcttc
      781 tttggacctt ggatccttgg atacatccga cagagatcgg gtgccccagc cgcgttatat
      841 gtgtgcgccc tggttgtgct gatcgccccg gctctggtga gttatttgcg gtttaccaac
      901 aaacaaatgc cttggaaata gaggaaccaa gggcatcgct ttattcagaa agccgaagag
      961 atcagtttaa accgcagctt acatagataa ccaaagcaga ggagtgagcg gtgccaccgc
     1021 aaggattcat caaaaaagcg atcaccgagc ccggaaaccc tcctcctcta ctctgtgtaa
     1081 tttcaccccg aaagatttcg ggggctgtgg ctttaaattc tttcttaaat ctcccatgtc
     1141 agatcgaatc atttccatct ccgccgctcc ctacgacgga ttcgagtttc cggcgatgct
     1201 ggatagcatc gcttcctgtg gggctcaata cgttgagccc gcattcatcg tcggttatac
     1261 ggaaccctt
//
LOCUS       DATMKH010000478         1171 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4059203, whole genome shotgun sequence.
ACCESSION   DATMKH010000478 DATMKH010000000
VERSION     DATMKH010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4059203"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..909)
                     /locus_tag="VNV63_05725"
     CDS             complement(<1..909)
                     /locus_tag="VNV63_05725"
                     /inference="COORDINATES: protein motif:HMM:NF019135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HXC62153.1"
                     /translation="MAATEDNSQTLWLATFGGGLLKFDREHGVFIRYRNDPENPESIT
                     EDRVMSLCGDREGNVWVGLYVSEPNFFRTEKASFMPLRVMRSNPRSHGENIVGAVYED
                     HDQILWMATTGALNRINRRSGESTAYQPPGQGLSSDVIAITEDRGGGLWIGTYGAGLS
                     RFDRRTGRFKTYLHQPDNPSSLSSNIVSRVLIDHAGTMWVGTYNGLDRFDPRNESFVV
                     YKQDTKNDDEVYANFTEDKSGFLWMGGWGGLNRFDPKTAQFTVYAHRVDDPSSLSDNT
                     VTSTYVDHSGNLWVGTENGLNQLNRES"
BASE COUNT          274 a          341 c          267 g          289 t
ORIGIN      
        1 gctttcacga ttcagctggt tcaagccatt ttccgtgccg acccaaaggt ttcccgaatg
       61 gtctacataa gttgaggtaa cggtgttgtc gctcaaagaa ctggggtcat ccactctgtg
      121 tgcatagacg gtgaattgag cagtcttcgg atcgaaacgg ttcaaacctc cccagccgcc
      181 catccagagg aacccgcttt tgtcctcagt aaaattggca tacacctcat cgtcattctt
      241 agtgtcctgc ttgtagacaa cgaagctctc gttccgaggg tcaaatcgat caagaccatt
      301 gtaggttccg acccacatgg taccggcatg atcgatcagc acccggctca cgatgttgct
      361 gcttaaactg gatggattgt ctggctggtg cagataggtc ttaaatcgcc cagtcctccg
      421 atcgaaccga cttaatcccg cgccataggt tccgatccag agaccaccgc cccgatcttc
      481 ggtgatcgca attacatcac tcgacagtcc ttgtccggga ggctgataag cggtggactc
      541 tccagatctt cgattgatgc gattcagcgc tcctgtcgtc gccatccaca aaatctggtc
      601 gtggtcttca tagacggccc ctacaatgtt ttcgccgtgg ctgcggggat tgcttcgcat
      661 aacacgcaga ggcatgaagg atgctttttc agtacggaaa aagttgggct cgctcacata
      721 caagcccacc caaacatttc cttctcggtc cccgcacagc gacatcacac ggtcttctgt
      781 gatgctctca ggattctctg gatcgttccg ataccgaatg aaaacgccat gttccctgtc
      841 gaatttcaac aaccctcccc cgaaggtcgc caaccaaagc gtttggctgt tatcttcggt
      901 tgccgccatg aatccagcgg gtaattcctt accagacttc ctgtcataga ttgaataagg
      961 aatcaggcta tttgtggatc ggtccagaac agctagtccg ctaccactgc tgttcgtgat
     1021 gtacgtaatc caaaagacgc caaggtgatc ttcatagaac gaaattaacc ttagaccctc
     1081 actcagacgg acccgcgaga gaactcttcc actctcacga tcgaactcgt ccagattctc
     1141 cccgtcggct acccagaatc ggccagtccg a
//
LOCUS       DATMKH010000479         1029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3729973, whole genome shotgun sequence.
ACCESSION   DATMKH010000479 DATMKH010000000
VERSION     DATMKH010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3729973"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="VNV63_05730"
     CDS             complement(<1..190)
                     /locus_tag="VNV63_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62154.1"
                     /translation="MTIENTQTGTGRGTAATLVFIDREQLLLDLVIGALQSDSLKIYG
                     ATDTDRGRQLVQMYSPTMV"
     gene            345..>1029
                     /locus_tag="VNV63_05735"
     CDS             345..>1029
                     /locus_tag="VNV63_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62155.1"
                     /translation="MRFRNLLAAVSVFAAVSFTSLSFSAQPEVYSPPTVFVRGLMMLQ
                     LEQKDGLHITLPDAPGHNANITFVMRDGQRRVVPFKGHNVINGSNSESSPVVKVPELI
                     GLKELFGNGITPKLDHAPNSVSIPWSSVRTVSTDKVSNVRYTFVRKDNGEEIQSFRPR
                     NVAELIRIELTSFGSLDFNLAKADIDVSKVKEIWIEQVPKDMTNMDVYREHFHHYLHY
                     IDRPASQTFD"
BASE COUNT          254 a          271 c          257 g          247 t
ORIGIN      
        1 caaccatggt tggagagtac atctgcacca gctgccgccc ccgatccgtg tcggtcgccc
       61 catatatttt gaggctgtcc gactggaggg ctccgatcac cagatccagc agcagctgtt
      121 ccctgtcgat aaagaccagg gttgccgcgg tcccgcgtcc cgtcccagtt tgtgtgtttt
      181 caatggtcaa tttgttctgc ctcactcagg aagacgtaaa acaccgccca ttccgccctc
      241 gtcccgcaaa aataaagttt gggtggtgag accatctttt ctcgtctctt tgaaatgaac
      301 ccaaacgaat aagccggccg gctgattcaa caaaaggaga cgaaatgaga tttcgaaatt
      361 tgttggcagc cgttagtgtg ttcgcagcag tgtctttcac gtctttgagt ttttccgcac
      421 aaccagaggt atacagtccg cccactgtgt ttgtccgggg tttaatgatg ctccagttgg
      481 aacagaagga tggtctgcac attacccttc ccgacgcgcc tggacataac gccaatatca
      541 cgtttgtcat gagggatggg caacggcgtg tggttccttt taaaggacac aatgtcatca
      601 acggttccaa ctcggagagt tcgccggtcg tgaaggttcc ggaactcatc ggattgaagg
      661 aactcttcgg caacgggatc acaccgaaac tggatcacgc gccgaacagc gtttccattc
      721 cgtggtcgag cgttcggact gtcagtacgg acaaagtttc aaatgttcga tataccttcg
      781 tccggaaaga caacggcgaa gagattcaaa gtttccggcc caggaacgtc gccgagttga
      841 tccgaatcga gttaacttcg ttcggaagcc tggacttcaa tctcgcaaaa gcagatattg
      901 atgtatcaaa agtaaaggag atctggattg agcaggtgcc caaagacatg acaaacatgg
      961 atgtctaccg ggagcatttt catcactacc tccactacat cgatcgtccc gcgagccaaa
     1021 ccttcgacg
//
LOCUS       DATMKH010000480         2218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4060883, whole genome shotgun sequence.
ACCESSION   DATMKH010000480 DATMKH010000000
VERSION     DATMKH010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4060883"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1839
                     /locus_tag="VNV63_05740"
     CDS             <1..1839
                     /locus_tag="VNV63_05740"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010527204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HXC62156.1"
                     /translation="AEDSVPPTIPLRDFFRNPETTGYELSPSGALIGFLKPVDNRLNI
                     FVQPKSGGEAKQITQVKDRDIRTFFWKGDNFLLYLKDNGGDENFHLYVSRSDGTGERD
                     LTPFDGVRAEMIDDLEDHPTDLIVGLNKRNKEVFDAYRLNVENGDLKMIAENPGNISS
                     WTTDHDGNIRVATTTDGVNTSLLYRETEKDAWKTVITTNFKESFNPQFFTFDNKALYG
                     VSNVGRDKAAVVEFDPETGKETKVLFAHPEVDVSGLHFSRKRKVTTSATYTTWKEERK
                     FFDPESEALDKTLGEKLPGYDVYVVSANKEEDMFIVRTITDRSLGAFYLYERKSGDLT
                     KLADRNPWLKEEQLSEMKPIEYTSRAGLTIHGYLTLPKGKPAKNLPMVVNPHGGPWAR
                     DEWGFDPEVQFLANRGFGVLQMNFRGSTGYGRKFWEAGFRQWGESMQDDITDGVAWLV
                     HQGIADPKRVAIYGGSYGGYAVLEGLTKTPDLYAAGVDYVGVSNLFTFMKSVPPYWKP
                     FLEMMYEMVGNPEKDKTLFEANSPALNANRIKAPLFVAQGAKDPRVNINESNQIVEAL
                     KKHGVDVEYMVKEDEGHGFHNEENRFSFYEAMEKFLAKYLATTRSS"
     gene            complement(1903..>2218)
                     /locus_tag="VNV63_05745"
     CDS             complement(1903..>2218)
                     /locus_tag="VNV63_05745"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HXC62157.1"
                     /translation="LESDNCPFISDFSLWEVATLAGLGRLKLDQKFEEWLLLAAKPGI
                     VRILPFSVEIAVELASLPDRLHRDPADRAIVATARAHGLPVMTRDRKILSSGLVQKWK
                     PK"
BASE COUNT          570 a          572 c          610 g          466 t
ORIGIN      
        1 gccgaagaca gcgttccgcc gacgattccg ttgagggact ttttccgcaa tcccgaaacg
       61 accggttatg agctctcccc cagcggtgcg ttgatcgggt ttctgaagcc tgtcgataac
      121 cggctgaata tcttcgtcca accgaaatca ggcggagaag cgaaacagat cactcaagtg
      181 aaagaccggg atattcggac ctttttctgg aagggagaca actttctttt gtacctgaaa
      241 gacaatggcg gggacgaaaa ctttcacctt tacgtgagcc ggagtgacgg taccggcgaa
      301 cgggacctga caccgtttga cggggtcaga gcagagatga tcgatgacct ggaagatcac
      361 ccgactgact tgatcgtcgg cctgaacaaa cggaacaaag aagtctttga cgcctaccgg
      421 ctgaacgtcg agaacggcga tttgaaaatg attgccgaaa acccgggaaa catttcatcg
      481 tggacaaccg atcacgacgg gaatattcgc gtcgccacga caaccgacgg cgttaatacg
      541 agtttgctct atcgggagac cgagaaggac gcctggaaga ccgttattac gacgaacttc
      601 aaagaaagct ttaaccctca attttttacc ttcgacaaca aagcgctcta cggtgtttcg
      661 aatgttggcc gggacaaagc cgcggtagtc gagttcgatc cggaaaccgg taaagagacg
      721 aaggtgctgt ttgcacaccc ggaagtggac gtctctggtc tgcacttttc cagaaaacga
      781 aaagtgacca catccgcgac ctacaccacc tggaaagaag aacgaaagtt ttttgatcca
      841 gaatctgagg cgctcgacaa gactctcggc gagaaattgc ccggttatga cgtttacgtt
      901 gtctctgcca acaaggagga agacatgttc atcgtccgca ccattaccga ccggtccctg
      961 ggggcattct acctctacga gagaaaatcg ggtgatctga cgaagctcgc ggaccgtaat
     1021 ccctggctga aagaagagca attatccgag atgaagccaa ttgagtacac ctcgcgcgct
     1081 ggtcttacga tccacggata tctcacctta ccaaaaggca aaccagcgaa gaacctgccg
     1141 atggtggtca atccgcatgg cgggccctgg gcgcgcgacg aatggggctt cgatcccgag
     1201 gtgcagttcc tggccaatcg cggcttcggc gtgttacaaa tgaacttccg cggttcgacc
     1261 ggctatggtc gcaaattctg ggaggcgggc ttccggcaat ggggagaaag tatgcaggat
     1321 gatatcaccg acggggtcgc ttggttggtt caccaaggga tcgctgatcc aaagcgggtc
     1381 gcgatttacg gaggaagtta cggcggatat gccgtgctgg aaggtctgac caagacccct
     1441 gatctgtatg cggcgggagt cgattacgtt ggggtctcca acctcttcac tttcatgaaa
     1501 agcgtgcctc cgtactggaa gccctttttg gaaatgatgt atgaaatggt cgggaatccg
     1561 gagaaggata aaacgctgtt tgaggcaaat tctcccgcct tgaatgccaa ccggatcaag
     1621 gcccctctct tcgtagcgca gggagcgaaa gatccgcggg tgaatattaa cgagtcgaat
     1681 cagatcgtcg aagcgctgaa aaagcacggt gttgacgtgg aatacatggt caaagaagac
     1741 gaaggccacg gttttcacaa cgaggagaat cgcttttcgt tctacgaagc gatggaaaaa
     1801 tttttagcga aatatctcgc gacgaccagg agttcctagg aaatccgcat ttccccgatc
     1861 cacgccaacg ccgagcgccg aacgccgaac gcaatgggca gtctatttcg gcttccattt
     1921 ttggaccagg cctgacgaaa gaattttcct atctcgtgtc atgaccggaa gcccgtgtgc
     1981 cctggctgtg gccacgatgg ctctgtccgc aggatcccga tgcaatcggt ccggcaatga
     2041 cgccagttcg accgcgattt ccacggagaa aggaaggatt cgtacgattc ccggcttagc
     2101 ggctagcagc aaccattctt caaacttctg gtccagcttc aggcgaccca atccagcgag
     2161 ggtcgccact tcccagaggc taaaatctga gatgaacgga caattatcgg actccagc
//
LOCUS       DATMKH010000481         1140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1508318, whole genome shotgun sequence.
ACCESSION   DATMKH010000481 DATMKH010000000
VERSION     DATMKH010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1508318"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="VNV63_05750"
     CDS             <1..488
                     /locus_tag="VNV63_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62158.1"
                     /translation="SHGVVMTQANTRKDSASEIRLVVEVDGSPVGFLNLDIDRLWPLI
                     NHGKRDTVPVEWMDQAKFESITCSAVMKRMVSRIEGRLYQTLGDEMVKAGLDVENFNL
                     KAQAAAQAFGTTKADIENLAAETGRTPQDFYSFFWDYLTADREVTDLKKEWRANGTAP
                     R"
     gene            complement(439..882)
                     /gene="queF"
                     /locus_tag="VNV63_05755"
     CDS             complement(439..882)
                     /gene="queF"
                     /locus_tag="VNV63_05755"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HXC62159.1"
                     /translation="MEFLISNLQYIGSVTDYTEEHAKSGIDAELPAIECWSNQFPGYD
                     ITIAMPEFTSICPKTGLPDFGTITIRYRPGKLCLELKSLKEYFTAYRNLGIFYENVVN
                     RILRDVVKACEPVSAKITGDFTARGGMHSVIEAQYHSPSTPSSSR"
BASE COUNT          272 a          293 c          312 g          263 t
ORIGIN      
        1 caagccacgg agtggtcatg acacaagcca acaccaggaa agactcggca tcggaaatac
       61 gcctcgtcgt ggaagttgat ggcagccctg ttggcttcct gaatctcgat atcgatcgtc
      121 tctggccgct gatcaatcac ggcaagcggg acacagtgcc ggttgaatgg atggaccagg
      181 cgaagttcga atccattacg tgttcggcgg taatgaagcg gatggtctcc cggatcgaag
      241 gccgccttta tcagacgctc ggcgacgaga tggtgaaggc cggacttgat gtggaaaact
      301 tcaacctgaa agcgcaggct gcggcgcagg cgtttggcac gacgaaagcg gatatcgaga
      361 atctcgcggc ggaaaccggt cgaacgccac aggacttcta ttcattcttt tgggattacc
      421 tgactgccga tcgggaggtt accgacttga agaaggagtg gagggcgaat ggtactgcgc
      481 ctcgataacc gaatgcattc ctccccgggc cgtgaagtct ccggtgatct tggccgacac
      541 gggctcgcac gccttgacaa catcccgcag gattcgattc accacattct cgtagaagat
      601 tccaaggttc ctataggccg taaagtattc ctttagagat ttcagttcaa ggcacagctt
      661 accaggacga tagcggatcg tgatggttcc gaaatcgggc agtcctgtct tcggacaaat
      721 cgaggtaaat tcgggcatcg caatggtgat gtcgtaaccc ggaaattgat tggaccagca
      781 ttcgatagct gggagttcgg cgtcgatgcc ggatttggcg tgttcttcgg tgtagtcggt
      841 cacggaacca atatattgca agttagagat cagaaattcc aaaagtttct catccgaatg
      901 aaaactcttg gagtttccga cttaaaattt caaatagaaa gaccctcaga cgcttcttgc
      961 atctgagggc cttgttccct agtccccttc atcgccgcac acgcggttcc acccgtaaac
     1021 gacttcctgt tcgttgagga atccttcctc tgggccagct tcctgctacc ggatgcggcc
     1081 gactaccgtc cctggctgga gtagccaaaa cgctactagg aataattgtc tcgtctgaga
//
LOCUS       DATMKH010000482         1001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_226140,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000482 DATMKH010000000
VERSION     DATMKH010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226140"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            275..>1001
                     /locus_tag="VNV63_05760"
     CDS             275..>1001
                     /locus_tag="VNV63_05760"
                     /inference="COORDINATES: protein motif:HMM:NF015210.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HXC62160.1"
                     /translation="MSVPPEAMMPTHKVNLDALIRREDFESGTAESSAGKEPIFKIED
                     LKQDRMYFKVLRKPDFQRTTNNWTPEMIVDLVKSWLDGSLVPALILWHSKDSGKVFVI
                     DGAHRLGALIGWVNDDYGDGAISRSFFDEILPDQVKFHNQTKQLMEQEIGSFEELYRI
                     GLNPTSDDDHEDVRRGRAIATVQPAIQRVEGDAQTAEKSFLKINSNPAMIDPTDLDVI
                     RARRKPNAIATRALMRAGTDYFSK"
BASE COUNT          263 a          266 c          266 g          206 t
ORIGIN      
        1 cgagaaactc accaaatctg tcatcccgag ataatctgtc atcccgagcg cagtcgaggg
       61 acctggcgtt tccccaccgc catcaccccg agccgagcaa tccgattggc gccctcagtc
      121 gcaacgcgaa gtctgaggag cccgcccacg ggcgctgcca tgaaagacaa aagctagcct
      181 ggcgtcctcg ttctcacatt tacaacgaac ctaaaggcgg gccagccacg gacagcggga
      241 gcgcgtctag cgcactctgt agcgggggtg tacgatgagc gttcccccgg aggcgatgat
      301 gccgacccat aaagtaaacc ttgatgccct catcaggcgt gaagattttg agtccggtac
      361 cgcggagagt tcggctggaa aagaaccaat cttcaagatt gaagatctga agcaagatcg
      421 catgtacttc aaggtgctgc gaaaacctga tttccagcga acgacaaaca attggacacc
      481 ggagatgatc gttgatctgg taaagagttg gctggatggg agccttgttc ctgctcttat
      541 tctctggcac tccaaggata gtgggaaagt tttcgttatt gacggagcac accgtttggg
      601 agcactgatt ggatgggtca atgacgatta cggtgacggc gcaatttcgc gctccttctt
      661 cgacgaaatc cttcccgatc aggtcaaatt tcataatcag acgaaacagt taatggagca
      721 agagatcggc agttttgaag agttgtatcg cattggactt aaccctacct cggatgatga
      781 ccacgaagat gttcgccgag gtcgggcaat tgctacggtt caaccagcaa ttcaaagagt
      841 agaaggggac gctcagacag ccgaaaaatc cttcctgaaa atcaactcca atccagccat
      901 gattgaccca actgaccttg acgtgatccg ggcgcggcga aagccgaacg ccatcgccac
      961 gcgggctttg atgagagccg gaacggacta cttctcaaaa c
//
LOCUS       DATMKH010000483         1777 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1822606, whole genome shotgun sequence.
ACCESSION   DATMKH010000483 DATMKH010000000
VERSION     DATMKH010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1822606"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..996
                     /locus_tag="VNV63_05765"
     CDS             <1..996
                     /locus_tag="VNV63_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009381897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HXC62161.1"
                     /translation="SKEWVYRQYDHMVQTNTIVQPGSGAAVIRIKGRDQALALTVDGN
                     SLYCLLNPYMGAVIAVAEAARNLVCAGAKPIAVTDCLNFGNPEKPEVMWQFALAIEGI
                     TDACKQFGLPVVSGNVSFYNETNGIDIYPTPIIGMIGLMEPARPMTSWFKTPGDRIVL
                     LGDTKEDLGGTEYLRQIFSNERGTPPFIELEKEAALHTLCLSAIKSGLIKSAHDCSEG
                     GFAVAIAESCITCEDFIMGAEVTLSDRKIRRDALLFGESQSRILATVPEEKLPELERL
                     AAQHKVPFEVIGQAGGDRLRISVHDSSSDRTSCLIDLEADKIAVTWRNAISKVLE"
     gene            993..>1777
                     /locus_tag="VNV63_05770"
     CDS             993..>1777
                     /locus_tag="VNV63_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HXC62162.1"
                     /translation="MSKKEMRSDKFHEECGVIGVYNHPEAANLAYLGLYALQHRGQEG
                     SGIVSYDQKEFHLEKGVGLVADVFSKERMERLPGTMAMGHNRYSTAGSNDFKNVQPLM
                     VNYALGTLALAHNGNLTNASYLRDELEAYGAIFQSTTDSEVMIHLIAHSKEKTLPDRI
                     VDALRQVKGAYSVIFLSEQGLIGVRDSYGFRPLSLGRIKDTWLLASETCAFDLIEAEF
                     VRDIEPGEMVVINGNGVQSYHPFPQTKLASCIFEYVYFARPDS"
BASE COUNT          406 a          444 c          521 g          406 t
ORIGIN      
        1 agcaaggagt gggtgtatcg tcaatacgat cacatggtcc aaacaaatac gattgtccag
       61 ccgggctcgg gcgcggctgt gatccgaatc aaaggaagag atcaggccct ggcattgacc
      121 gtggatggga acagcctgta ctgtctgctc aatccctata tgggggccgt gatcgcagtc
      181 gcggaggcgg cccggaatct cgtctgcgca ggcgcaaaac ccattgccgt gaccgactgc
      241 cttaactttg gcaatcctga aaaaccggag gtgatgtggc agtttgcttt ggcaatcgaa
      301 ggaatcacgg atgcctgcaa acagtttggg ttgccggtag tgagcggcaa tgtgagcttc
      361 tataatgaga caaacgggat tgatatctat cccacgccga tcatcggcat gatcgggctg
      421 atggagcctg caagaccgat gacctcttgg tttaagacgc ctggcgatcg aattgtcctg
      481 ctcggtgata cgaaggaaga tctgggtgga acggaatatc ttcgtcaaat cttctcgaac
      541 gagcgtggaa cccctccttt tattgagttg gaaaaggagg ctgctctgca taccttgtgt
      601 ctctctgcga tcaagtcggg cctgataaag tccgcccatg actgctcgga agggggattc
      661 gcggttgcca tcgccgagtc ctgcatcacg tgcgaagatt ttataatggg ggccgaggtg
      721 accctttcgg accggaagat ccggcgggac gcgcttctct ttggcgagtc gcaatcccgt
      781 atactggcga cggttcccga agagaagttg ccggagctgg aaaggttggc cgcacagcat
      841 aaagtcccgt tcgaggtgat cggccaggcg ggtggagata ggcttcgcat cagcgttcac
      901 gattcatcgt ccgatcggac atcttgtttg attgacctag aagctgataa gattgcagtg
      961 acatggcgga atgcgatttc caaggtgctc gaatgagtaa aaaagaaatg agatcggaca
     1021 agtttcatga agagtgtggc gtgatcgggg tctacaacca tcccgaagcg gccaacctgg
     1081 cctatctggg gctctatgcg cttcagcatc gaggtcagga aggaagcgga atcgtgtcct
     1141 atgatcagaa ggagttccac ctggagaaag gcgtaggact cgtggcggat gtcttctcta
     1201 aagaacgcat ggagcgtctt ccgggaacca tggccatggg tcacaatcgt tactccaccg
     1261 ccggctcaaa cgatttcaaa aatgtccagc cgctgatggt caattatgcc ctggggacct
     1321 tggccctggc ccataacgga aatctcacca atgcctctta ccttcgggac gagttggaag
     1381 cctacggtgc gatcttccag tctacaacag atagcgaggt gatgatccac ctcattgccc
     1441 attccaaaga gaagacattg ccggaccgga tcgttgacgc tttgcgccag gtgaagggcg
     1501 cctactcggt tatattcctc tcggagcagg gcttgatcgg tgtgagagac agttatggtt
     1561 tccggcctct gtctttaggg cgcatcaagg acacctggct gctggcctcg gagacctgtg
     1621 catttgatct gatcgaggcc gagttcgtcc gggatatcga gccgggcgag atggtcgtga
     1681 tcaacgggaa tggtgttcaa tcgtaccatc ccttcccaca gaccaagctg gcctcctgta
     1741 tttttgagta tgtatatttc gcacgccccg acagcca
//
LOCUS       DATMKH010000484         2611 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3420026, whole genome shotgun sequence.
ACCESSION   DATMKH010000484 DATMKH010000000
VERSION     DATMKH010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3420026"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..956)
                     /locus_tag="VNV63_05775"
     CDS             complement(<1..956)
                     /locus_tag="VNV63_05775"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008765 -
                     UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HXC62163.1"
                     /translation="MMLEEILKGLDDAKIHPSFQGPSHQEIRGLVYDSRQVQPGDLFV
                     AVRGYRADGHRYLAEAVSRGATAAVIEDAESARSVSIPLVLVPDSRQALALLANRFYG
                     YPSRRLTMIGVTGTNGKTTTSYLIKRIFEAAGRKMGLLGTVTYEIGKESLTPPNTTPE
                     SLDLQRLLSRMVEEGLSGAVMEVSSHALAMDRVLGCEFDTAVFTNLTQDHLDFHRTME
                     DYFSAKQRLFTELGREAIKSTRKTAVINLDDSWGERLVQLTRTPIYTYGLTRHNGADI
                     TAKEVQSDWNGIRFTAITPVGDFPIESVLIGKYNLYNLLAAIG"
     gene            complement(953..>2611)
                     /locus_tag="VNV63_05780"
     CDS             complement(953..>2611)
                     /locus_tag="VNV63_05780"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HXC62164.1"
                     /translation="SIFLEGERGTIYDRRGRVLATNLEVPSIYAVPTSIQGSDQVGLE
                     LARVLKSDLTSVKRRLREDRNFVWIARKVTPERAKAVSDLKIDGIGFVMESQRFYPNR
                     HLMGHLLGFAGMDNQGLEGVELAYDSYLRGEKGLLWMERDALGETIFPSGLGHVAPSR
                     GRDIVLTIDEVIQYITEQELDRVVQQVSAVSGSIIVMNPKTGEILAMAVRPEFNPNIL
                     SRHDPSEWRNRAITDLYEPGSTFKIVTAATALEEKLVTPAERINCEEGSFQIGGEVFH
                     DPVKEGVLTFSEVIQRSSNIGTIKIGTRLGAERLYQYAKTFGFGEKTGIDLLGETAGT
                     LRSPHNWSKRSLASIAIGQEVAVTPIQLITAASAVANGGQLMRPYLVSKITEHDGPVI
                     QTIVPELRRRVISQETARQLTQILEGVTRKGGTGEKAALETYSVAGKTGTAQKADSKT
                     KAYSSDRFVSSFVGFVPAEDPEIAILVVVDEPKGVSWGGSVAAPVFKNVAEQTLLYLG
                     RVSPIQERKLVVSKTDPSESMPGSSGKYIPGPSGGLPGPSGGLQ"
BASE COUNT          599 a          786 c          634 g          592 t
ORIGIN      
        1 ccgattgccg caagaagatt gtagagattg tatttcccga tcaagaccga ttcgatcgga
       61 aagtcgccaa ccggcgtgat tgctgtaaag cggattccgt tccaatccga ttgaacctct
      121 ttggccgtaa tgtccgcccc gttgtgccgt gttaagccat aggtgtagat cggcgtccgg
      181 gtcaactgca cgagtcgctc tccccaggaa tcatccagat taatcaccgc ggtcttgcgt
      241 gtggatttta ttgcctctcg acccagctcc gtaaaaagcc gctgcttcgc cgagaagtaa
      301 tcctccattg tacgatgaaa atccagatgg tcctgggtca gattggtaaa cacggccgta
      361 tcaaattcac agcccagcac ccgatccatc gccaaggcgt gagaggagac ctccatcaca
      421 gctccggaaa gaccttcctc aaccattcgc gacaggaggc gctggagatc cagagactcc
      481 ggagtggtgt tcggcggcgt caaggattct tttccgattt catacgtcac agttcccaaa
      541 agccccatct ttcggccagc cgcctcaaag atcctcttaa tgaggtaact ggtagtcgtt
      601 ttcccattcg tcccggtcac gccaatcatg gtgagacgcc gggatggata cccataaaac
      661 cggttggcta aaagggccaa ggcttgacgt gagtccggaa ctaaaaccag cgggatcgaa
      721 acagaacggg cagactcggc gtcttcaatg accgcagcgg tcgcaccgcg actgactgct
      781 tccgccagat agcgatgacc atctgcacga taaccccgaa cggccacaaa gagatccccc
      841 ggctgaacct ggcgtgaatc gtagaccaga cctcgaatct cctgatgcga aggcccctgg
      901 aaactcggat ggatctttgc gtcgtccaga cctttcaaga tttcttccaa catcattgta
      961 atcccccgga ggggcccggt aatcccccgg aggggcccgg tatatacttt ccggaagaac
     1021 ccggcatgct ttcggagggg tccgtttttg aaaccaccaa tttacgttct tgaatgggtg
     1081 atactcgtcc caggtataaa agcgtctgct ccgctacatt tttaaacact ggcgccgcca
     1141 cacttccacc ccaggaaacc cccttcggtt catccactac caccagaatc gcgatctccg
     1201 gatcctcggc tggtacaaat ccaacgaagg aactgacaaa acgatccgaa ctgtatgcct
     1261 tggttttgga atcggccttc tgggcggtcc ccgtcttccc cgcaaccgaa taggtctcta
     1321 atgctgcttt ctcacccgtg ccacccttac gggtaacacc ctccaagatt tgtgtcagtt
     1381 gtcgtgccgt ttcttgactg attacccgtc tcctaagctc cgggacgatt gtctggatga
     1441 ctggaccgtc gtgttcggtt atcttagaaa caagataggg ccgcattaac tgacccccat
     1501 tggcaacggc tgatgccgcc gtgatgagct gaatcggggt caccgcaacc tcctgcccga
     1561 ttgcgatcga agcaagagag cgtttggacc agttgtgagg cgatcgaagt gtgcctgccg
     1621 tctctccaag aagatcaatc ccggtcttct ctccaaaccc aaacgttttg gcgtactgat
     1681 aaagtcgttc ggccccgagc ctcgtaccta ttttgattgt cccgatattg ctagaccgct
     1741 ggattacctc cgaaaaagtc agcacacctt ctttgaccgg atcgtgaaat acctccccgc
     1801 ctatctggaa agatccctct tcgcaattaa tccgctctgc cggcgtcacc agcttctctt
     1861 ccaatgcagt cgcagccgtg acgatcttga acgttgagcc aggctcgtac aaatccgtga
     1921 tcgcacgatt acgccactcg gacggatcat gccgggagag tatattgggg ttgaactcgg
     1981 gccgaaccgc cattgccagg atctctcccg tcttgggatt catcacgatg atggatccgc
     2041 taacggccga gacctgttgg accacacgat ccagttcctg ttcggtaatg tactgaatca
     2101 cctcatcgat cgtaagcaca atgtctcttc cgcgagacgg agcgacgtga ccgagcccgg
     2161 acgggaagat cgtctctccg agcgcgtctc gctccatcca gagcaagccc ttctctcctc
     2221 ggagatagga gtcatatgcc agctccaccc cttcaaggcc ctgattatcc attccggcga
     2281 atccaagaag atgacccatg agatggcggt tgggataaaa acgttgactc tccatcacaa
     2341 acccgatccc atcgatcttc agatcggaaa ccgccttcgc acgttccggt gtcaccttcc
     2401 gggcgatcca aacgaagttt cgatcttccc ggagccgtcg tttcacagac gtcagatcgc
     2461 tcttcaaaac acgagccagt tctaaaccca cctgatccga tccctgaatc gaagtcggca
     2521 cggcatagat cgaggggact tcgagattcg tggccagaac tctcccccgg cggtcataga
     2581 tcgtcccgcg ttctccttca agaaaaatgg a
//
LOCUS       DATMKH010000485         4014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_228010,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000485 DATMKH010000000
VERSION     DATMKH010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4014
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228010"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="VNV63_05785"
     CDS             complement(<1..407)
                     /locus_tag="VNV63_05785"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXC62165.1"
                     /translation="MKRVRVMIVDDSAFYRQAIANILRSSDHLEVVGSVANGSEAIKS
                     LPLLKPDVITLDLDMPNMDGFTFLRWLMVNQPMPVLVISSHSENNNVFRALEMGAVDF
                     VAKTTEKASLDFLRLRDELVSKIETITMVTGEK"
     gene            complement(404..1288)
                     /locus_tag="VNV63_05790"
     CDS             complement(404..1288)
                     /locus_tag="VNV63_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HXC62166.1"
                     /translation="MNEELVGVEKQIELPVDVFRILRDMLYEQSGVYLNDNSKFFLES
                     RLQHSVRRQQFDNFKDYYYFLKYDRKKDEELTNFIDLLTIHETFFFREERQLKAFGEE
                     ILPELAERKKKQRSLRIWSAGCSTGEEPYTIAMLIAEQDALKGWDIEIFASDISQRVL
                     QSARRGIYQPSAFRSTDPRYIANYFHTEGTGYRIDDRIKQSVTFLGLNLLDTCKLALI
                     SMMDVIYCRNVIIYFDKAAKKKVVAVFHQKLKNHGYLLLGHSESLINISTAFALRHFK
                     HDLVYQKIECADPGLPKP"
     gene            complement(1294..3303)
                     /locus_tag="VNV63_05795"
     CDS             complement(1294..3303)
                     /locus_tag="VNV63_05795"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HXC62167.1"
                     /translation="MEDQELTIALQDQEIETRLRAIAEVERRQDIQYIPTLLKLLGDP
                     EWRVRKTAVSSLIQLKRDSALAYALIDLLQSPEENMGRQNAVVEVLIGIGSPAVSALI
                     QALPRVNKEIRKFIADILGEIGDKTATSCLIERLNDEDENVKLAAIEALGKLKDSRAV
                     ESLIVLLRSESHLLGYAAIKALEQIQDSRAVEPIVNVLGKKLLERAGLEALGQLGDLR
                     ALNPIVMALQLGNKKVKESAIKALVGLHDRPVMNSEVKIISRLREVYSKEICLFLMEA
                     IESPDERTQLAVIIVLGWLGEAKAAKCVVKFLDGPFRDKAIATLVSMRRAAIDPLLQE
                     IHKANETTRQGIAKVFGEIGDRREIPTLIKFLSDSNGHVRREAAIALGKINDPACTRA
                     LLKLLDDEYADVQATAIQVLSLFKRQKELLPAIFDLLSHPNAALRCNGIKLLGRMIIP
                     EAIPHLTFCLKDESPQVRRCAVESLCHFGTEEVNDLLMPCLADEDAGVRLATLSLLMK
                     RSTAQLLPHIESLMMDKNIWVRASVAKAIGLQRSPVAKPYLMTLINDKVGVVQIAAIE
                     ALAQFGDRAALPAILNLTTSKDSDVKKAAITALGQLGDSKVVEMVQSFLTDPHWGIRL
                     AATQCLGRLKAITARERLERLAQQDEDHLVRQTARFALEQLAERA"
     gene            complement(3304..>4014)
                     /locus_tag="VNV63_05800"
     CDS             complement(3304..>4014)
                     /locus_tag="VNV63_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62168.1"
                     /translation="DKTRYKREPDSLYGADDYIERHDIQNRLVSKIEELLGKRPMPPK
                     GMPAPLPTPAAPKPVPAPTLQGVDNMLLDEVTIYEPSLHALPTVGHAAKPEARAPVTP
                     PPIEIAKSQLDDSTVYEPGLHALPAGGHVAKQEPRSHATPAPVETSKSPPVAGDPAKH
                     EAAKRLARLILSDIALYNQKAVENGIRNNTFHQLLKSELEEGRKLYCSRVLPDVMATT
                     DYYNQAIEEFIQKRKGSG"
BASE COUNT          914 a         1081 c         1003 g         1016 t
ORIGIN      
        1 aatttttcac ccgtcaccat ggtaatggtt tcgatcttac tcaccaactc atctcgaagc
       61 ctcaggaagt ctaaggatgc tttctcggtt gtctttgcta caaaatctac ggcgcccatc
      121 tccagggcac gaaagacatt gttgttttcg gagtgtgagc tgattaccag aaccggcatg
      181 ggttggttaa ccattaacca tcgaagaaaa gtaaagccgt ccatattcgg catgtccaaa
      241 tccaaagtga tcacatccgg cttgagtaac ggaagggatt ttatcgcctc gctgccgttc
      301 gccacggaac caaccacctc caggtggtcg gaagacctta atatattggc aatggcctgc
      361 cggtaaaagg cagaatcatc cacgatcatg acgcggaccc gtttcatggc ttcggcaatc
      421 ccggatctgc acattcgatc ttttgataga caaggtcgtg cttaaaatgt cgcagagcaa
      481 aagcggtgga aatattgatc aaggattctg aatgaccgag aagaagataa ccatgattct
      541 ttaatttttg gtggaaaacc gcgaccacct ttttctttgc tgccttgtca aaatagatga
      601 tgacattgcg gcagtaaata acatccatca tgctgatcag agccagcttg caggtatcca
      661 aaagattcag ccccaaaaaa gtaacggatt gttttatccg atcatcgatg cgataaccgg
      721 ttccttccgt gtggaagtag ttggctatat atcgaggatc ggtggaccga aaggccgaag
      781 gttgatagat ccctcgacgc gccgattgaa gaacccgttg gctgatatcg cttgcaaaga
      841 tttcgatgtc ccatcctttg agggcatcct gctccgcaat cagcatagca atcgtatatg
      901 gctcttcccc ggttgagcaa cctgcactcc aaatacgaag agaccgctgc ttcttttttc
      961 gttctgccag ttccggcagg atctcttcgc caaaagcctt aagctgtctt tcttcacgga
     1021 agaaaaaggt ctcatggatg gtcaacagat caataaaatt ggtaagctct tcgtcttttt
     1081 tgcggtcata ttttagaaaa tagtaatagt ccttaaagtt atcgaactgt tgccggcgca
     1141 cgctgtgttg aagacggctc tccaaaaaaa actttgagtt gtcattgaga taaacgccgc
     1201 tctgctcgta aagcatgtcc cgaaggatcc ggaagacatc gacaggtaat tcgatttgtt
     1261 tttcgacgcc gaccagttct tcattcattt gtatcaggca cgctctgcca gttgctccag
     1321 tgcaaaccgg gcggtctgtc ggaccagatg atcctcgtcc tgctgtgcca gccgttccaa
     1381 ccgttcacgg gcagtgattg ccttcaaacg acccagacac tgcgtcgccg ccagtcgtat
     1441 tccccagtgg ggatcggtca agaaggactg gaccatttcg accaccttgg aatctcccag
     1501 ctggcctaat gcagtgatgg cggccttctt tacatccgaa tctttgctcg tggtcaagtt
     1561 taagattgcc ggcagcgccg ctcgatcacc aaactgagcc aatgcctcga tggccgcgat
     1621 ctgcactacc ccgactttgt cattgatcaa tgtcatcagg tacggtttgg ccactggact
     1681 acgctgtaat ccaatggcct tagccacact tgcacgcacc cagatgtttt tatccatcat
     1741 cagagattcg atatggggca agagctgtgc cgtgctgcgc ttcatgagga gcgagagcgt
     1801 ggccagtcga acacccgcat cctcatccgc caggcagggc atcagcaggt cattaacttc
     1861 ttcggtccca aagtggcaca aagattcaac cgcgcaacga cgaacctgag gagattcgtc
     1921 tttcagacag aaagtcaaat gcggaatagc ctcggggatt atcattctac ctagcagttt
     1981 gatgccgtta caacgaagag cagcgtttgg atgagataga agatcaaaga tagcaggcag
     2041 caactccttt tgcctcttaa agagacttaa tacttgtatc gcggtcgcct ggacatcggc
     2101 gtattcgtca tcgagtagct tcagcagagc acgggtgcag gcggggtcat tgatcttccc
     2161 cagagcgatc gcggcctccc gtcgaacatg accgtttgaa tccgataaaa atttaatcag
     2221 tgtcggtatc tcgcgtcggt ccccgatctc tccaaagacc tttgcgatcc cctggcgggt
     2281 tgtctcattc gccttgtgga tctcttgaag cagcggatcg atggcagccc gtctcatgct
     2341 gaccagggtc gcgatggcct tgtcccgaaa tggtccatcc agaaacttga cgacgcattt
     2401 tgccgccttt gcctcgccca accaacctaa gacgatgatg accgccaact gggtccgctc
     2461 atctgggctc tcgatcgcct ccatcaggaa aaggcagatc tctttgctgt acacctccct
     2521 caggcggctg atgatcttca cctccgaatt catcactgga cgatcgtgaa gaccgaccag
     2581 ggccttaatg gcggactcct tgactttttt gttgccaagc tgaagggcca tgacgattgg
     2641 attcaaggcc cgaagatcgc ccaattggcc gagcgcctcc aggcctgccc gttccaagag
     2701 cttcttcccc aacacattca cgatcggttc caccgcgcgg ctgtcttgta tctgttcgag
     2761 cgccttgatc gcggcataac ccagtaaatg gctctcgctc cgcaggagga cgattaaaga
     2821 ttcaacggcg cggctatcct tgagtttgcc cagggcctcg atggctgcga gtttcacatt
     2881 ttcatcttcg tcgttcagcc tctcgatcaa gcaggaagtg gcggttttat ctccgatctc
     2941 acccaagata tccgcaataa attttcggat ctctttgttg actcgaggaa gcgcctggat
     3001 caatgctgaa accgcaggcg agccgatccc gatcaaaacc tccaccacgg cattctgtct
     3061 ccccatgttt tcctccggcg actgaaggag gtctatcagc gcataggcca aagctgaatc
     3121 ccgttttaat tggatcaggg acgagaccgc ggtcttcctc acacgccatt ctggatcgcc
     3181 caagagcttt agcagggtcg ggatgtattg aatatcctgc cttcgctcca cctccgcgat
     3241 ggcccggagt cgcgtctcga tctcttgatc ttgcagtgct atcgtaagtt cttggtcttc
     3301 catctaaccg gagccttttc gtttctgaat aaattcttcg atggcctggt tgtaatagtc
     3361 ggtcgtcgcc atgacatcgg gtaagacacg cgaacagtag agtttgcggc cttcttccag
     3421 ctcgctctta agcagttgat gaaaggtgtt gttacggatg ccgttttcca ccgctttctg
     3481 gttatagagc gcgatatcgg atagaattaa acgggcaagc cgttttgcgg cctcgtgttt
     3541 ggctggatcg cctgcaacag gcggactttt ggaggtctcg acaggagccg gcgtcgcatg
     3601 agagcgtggt tcctgcttcg ccacatgccc gcccgccggc aaggcatgca atcccggttc
     3661 gtagacggtt gagtcatcaa gctggctctt tgcaatctcg atgggtggcg gcgtcacagg
     3721 agcgcgtgct tccggcttcg ccgcgtgccc gaccgtcggc aaggcatgca agctcggttc
     3781 ataaattgtt acctcatcaa gaagcatgtt gtccacccct tggagtgttg gcgctggcac
     3841 cggctttggt gcggctggag tcgggagggg agcaggcatc cccttgggag gcatgggacg
     3901 cttaccgagt aactcttcaa tcttggaaac cagacgattt tgaatatcat gccgttcgat
     3961 ataatcgtcc gctccataga gggagtccgg ctctcgttta taacgcgtct tatc
//
LOCUS       DATMKH010000486         1467 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2795551, whole genome shotgun sequence.
ACCESSION   DATMKH010000486 DATMKH010000000
VERSION     DATMKH010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1467
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2795551"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1146)
                     /gene="mqo"
                     /locus_tag="VNV63_05805"
     CDS             complement(<1..1146)
                     /gene="mqo"
                     /locus_tag="VNV63_05805"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017640010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HXC62169.1"
                     /translation="MATPAFTSYPDVVLVGAGIMSATLAVILKELDPSLKIEIYEVLG
                     SEAQESSNAWNNAGTGHAALCELNYTPQESDGSIDISKALQVNTEFDLSRQLWAYLVK
                     KGAIKDPQSFIHPVPHMSFVRGSENVAFLKKRFEALSAHHLYHGMEYSDDKKQIEKWV
                     PLVMEGRDPSEEVAATRMRTGTDVDYGALTKDLLDSLSNKEGFSIHFFHRVQDLRRDG
                     DLWTVQIRNEKSGEHWDVRAKFVFLGAGGGSLRLLQKSGIPEGRGYAGFPVSGIWLRC
                     DDPVIASRHDAKVYGKASVGSPPMSVPHLDTRHIEGKVSLLFGPYAGFSTKFLKHGSY
                     LDLFGSIDPENILPLLAVGRDNVALTEYLIGQVLESSEERFAALREFF"
     gene            complement(1163..>1467)
                     /gene="fumC"
                     /locus_tag="VNV63_05810"
     CDS             complement(1163..>1467)
                     /gene="fumC"
                     /locus_tag="VNV63_05810"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013895509.1"
                     /note="class II family (does not require metal);
                     tetrameric enzyme; fumarase C; reversibly converts
                     (S)-malate to fumarate and water; functions in the TCA
                     cycle; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HXC62170.1"
                     /translation="VTHSITIMTDGCTNFRKFLIEGTKPNLKKIKEYVDRSLMLVTAL
                     SPVIGYDKASKIAHYALDNDLTLKQAALKLGFVTEEEFDRVVDPAKMVKPYVAAAN"
BASE COUNT          335 a          404 c          382 g          346 t
ORIGIN      
        1 aaagaactcg cgcaacgcgg cgaaccgctc ctccgaggac tcgagaacct ggcctatcag
       61 atattcagta agagccacgt tatccctgcc gacagccagc agcggcagta tattttccgg
      121 atcgatggag ccgaagagat ccagatagga tccgtgcttg agaaacttcg tcgaaaagcc
      181 cgcataaggc ccgaaaagca gagaaacctt cccctcgata tgacgtgtat cgagatgcgg
      241 cacggacatc ggaggtgatc ccaccgaggc tttaccgtac accttggcat cgtggcggga
      301 agctatcacg ggatcgtcgc agcgcagcca gattccgctc accggaaagc cggcgtaacc
      361 ccgcccctcg ggaattccgg atttttgcaa caatcgcagc gatccaccgc cggcgccgag
      421 aaaaacgaat ttggctcgga catcccagtg ctcgccgctc ttttcgttcc tgatctgcac
      481 agtccacaga tccccatcac ggcgcagatc ttgcacgcga tggaaaaagt gaatggagaa
      541 tccttcttta ttgcttaggg aatcaagaag atccttggtg agggcgccat aatccacgtc
      601 agtgccggtc cgcatgcgcg tcgccgcaac ttcctcgctt ggatcacggc cttccatgac
      661 cagcgggacc cacttctcaa tctgcttctt gtcatcggaa tattccatcc cgtgatacag
      721 gtgatgggcc gaaagcgctt cgaagcgttt cttgagaaac gcaacatttt ccgatccgcg
      781 tacaaagctc atgtggggta cgggatgaat aaaggattga gggtctttga tcgctccctt
      841 tttcactaga taggcccaca gctgacggga aagatcgaac tcggtgttaa cctgcagagc
      901 cttggaaatg tcgatgctgc catcactttc ctgtggagtg tagttcaact cacagagtgc
      961 cgcatggccg gtgccggcgt tgttccatgc attcgagctt tcctgcgcct cactgcccag
     1021 gacctcgtaa atctctattt tcaggctggg atcaagttct tttaggatca ccgcaagcgt
     1081 agcgctcatg atccccgcac cgacaagaac gacatctgga tagcttgtga atgcaggcgt
     1141 tgccatagta ttcctcctgt tgtcagttgg ctgctgcgac gtagggcttg accatcttgg
     1201 ccggatcgac cacgcggtcg aactcctctt ccgttacaaa tcccaacttc aacgcggctt
     1261 gcttgagggt gagatcgttg tccagcgcgt agtgtgcaat cttcgatgct ttgtcatagc
     1321 cgatgacagg cgaaagagca gttactaaca tcagggaacg gtcgacatac tctttgatct
     1381 ttttcaggtt cggcttcgtt ccttcgataa ggaatttacg gaagttggtg catccatcag
     1441 tcatgattgt gatggagtga gtcacgt
//
LOCUS       DATMKH010000487         1012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1511841, whole genome shotgun sequence.
ACCESSION   DATMKH010000487 DATMKH010000000
VERSION     DATMKH010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1511841"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="VNV63_05815"
     CDS             complement(<1..401)
                     /locus_tag="VNV63_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HXC62171.1"
                     /translation="MSSVTLWHGLGRKTFGYWLIFPALLVILGLNLYPTIQGILTSFT
                     NQSLIDPGHSLYLGLDNYFKLLKEPVFQVALAHSLELTFFAVVLQVLLGLVMAHLLIQ
                     KVPGIHFFRSVAMITWVLPIIASVVMFRFMTL"
     gene            complement(568..>1012)
                     /locus_tag="VNV63_05820"
     CDS             complement(568..>1012)
                     /locus_tag="VNV63_05820"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HXC62172.1"
                     /translation="SGDVAKKILEEETGVAPLPLPPGGVQATYLEVKPISINAFSKDK
                     KDAWDLVKLLTSKEFVATANRIEGVNPPRKDVADLPDFKNDWWEQAFIAQLPTGVALA
                     PINWGLVINDITEALQKAIYKNATPEEAGKALYNTLEQRAKDNQL"
BASE COUNT          230 a          264 c          287 g          231 t
ORIGIN      
        1 agcgtcatga atcggaacat gaccaccgag gcaatgatcg ggagcaccca ggtgatcatt
       61 gcgacgctcc gaaaaaagtg aatgcccggg actttctgga tcaggagatg agccatcacc
      121 agaccaagca gaacctggag cacgaccgca aaaaaagtca gctccagaga atgcgccagc
      181 gccacctgga agacgggctc cttcaggagt ttgaaataat tgtcgagtcc gaggtagaga
      241 gaatggccgg gatcaatcag ggactgattg gtaaaagagg tcagaattcc ctgaatcgtc
      301 gggtagaggt tcaacccgag gatgacaaga agagcgggaa aaataagcca gtacccgaag
      361 gtctttctcc ccagaccgtg ccatagtgtg acggagctca ttctttgggt ctgacagtat
      421 gcctcaggct atgggattcg cctgagccgg tgattaattt aacatatcgt tctcaagcag
      481 acgcatagcg tctgcctaag aactttcaaa ccgcgcaatg agcctgtcgt tttggcccgg
      541 cggcggccgg acgttaattt tgccgggcta gagctgattg tctttggccc gttgctcgag
      601 cgtgttgtaa agcgccttgc cggcttcttc tggagtggcg ttcttgtaaa tcgccttttg
      661 cagggcctcg gtaatatcgt tgatgacaag gccccagttg atgggtgcca gggccacgcc
      721 ggtcggcagt tgcgcgatga aggcctgttc ccaccaatcg tttttgaagt cgggcaaatc
      781 cgcgacatct tttctcggcg gattgacgcc ttcgatccgg ttcgccgtgg ccacgaactc
      841 tttgctcgtc aaaagcttga cgagatccca agcatctttc ttatccttgc tgaaggcatt
      901 gatcgagatc ggcttcactt caagataggt tgcctggacg cctcccggcg gcaggggcag
      961 cggagcgaca ccggtctctt cttcgagaat cttcttcgct acgtcgccgc tc
//
LOCUS       DATMKH010000488         1186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4064609, whole genome shotgun sequence.
ACCESSION   DATMKH010000488 DATMKH010000000
VERSION     DATMKH010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4064609"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1145)
                     /locus_tag="VNV63_05825"
     CDS             complement(<1..1145)
                     /locus_tag="VNV63_05825"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HXC62173.1"
                     /translation="MGRLALTFFPVAIHIDHFGVKNPPIFPSGYVVQVARIDAELSVG
                     ALLHRQVVVKSLVLEDPVLNLTSDPDGPWNFENPQSKTSNETFPLGVISRVQIKRGQV
                     IASNLLPSDAAGPIFFEAHEVSCDLEQVNLMGIINPSSSSMDGQGSLTADLLRFGSME
                     ARNMVSKLLLESRHVSFVNVKAEIYGGKTDGELSFNLSGKNASFTSEARFSGINVAQL
                     LAAFTNGGGKMTGKMEGNVKLSGEIEHSSRPLAGIHGGGHVTVRNGQVPSLKLNANLM
                     KLAHFNDLGPAKNDPSSFNVITTDLELANQQISSKVIDIDGYGVDVDGSGSVSVSGSD
                     ELNYRGLAEITTKESFFTNTVARMSGASLKDGKLQFPFRVGGTISSP"
BASE COUNT          272 a          339 c          294 g          281 t
ORIGIN      
        1 atcgggctgc tgatcgttcc gccgacccga aagggaaatt gcaacttgcc atcctttagt
       61 gaggctccgg acatccgggc aacggtgttg gtgaagaagc tttccttggt tgtaatctcc
      121 gccaacccgc gatagttcag ctcgtcagag cctgacacgc tgacgctgcc tgagccgtcc
      181 acatcgactc cgtagccgtc aatatcgatc accttgcttg aaatctgttg attcgccagc
      241 tccagatcgg tggtgatcac gttaaacgac gagggatcat tcttcgccgg gccgagatcg
      301 ttaaagtgcg ccagcttcat caaattcgca ttgagcttga ggcttggaac ctggccgttt
      361 cgcaccgtta cgtgccctcc tccgtggatt cccgctagcg gccgggacga gtgttctatc
      421 tcccccgata actttacgtt tccttccatc tttccggtca tctttccgcc gccgttcgtg
      481 aaggccgcca aaagctgggc cacattaatc ccgctaaatc gcgcctcgct cgtaaaactt
      541 gcattctttc cggacaagtt aaacgaaagc tcaccgtcgg tcttcccgcc atagatttcc
      601 gctttaacgt ttacgaaaga aacgtgtctc gactccagga gtaatttaga caccatgttt
      661 cttgcctcca tcgagccgaa gcgcagaaga tccgctgtca ggctgccctg gccatccatc
      721 gaagaagacg aaggattgat gattcccatc aggttcactt gctccaggtc acaggagacc
      781 tcgtgggctt cgaaaaagat gggacctgct gcgtcggatg gcaaaagatt cgacgctatc
      841 acctggcctc gcttaatctg caccctcgaa atcaccccaa ggggaaaggt ctcgttggaa
      901 gtcttggatt gaggattctc aaagttccat gggccgtccg gatcggaagt caaattcagg
      961 acgggatcct caagcacaag agatttgacg accacctgcc gatgcagcag cgctccgacg
     1021 ctaagctcgg catcgatccg cgccacttgg acgacataac cggacggaaa aatcggaggg
     1081 ttcttcacac cgaaatggtc aatgtgaatc gctactggaa aaaatgtgag tgccagccgt
     1141 cccatttcga cttgcttccc agtttcttgt tgaagataag agatca
//
LOCUS       DATMKH010000489         1005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3423275, whole genome shotgun sequence.
ACCESSION   DATMKH010000489 DATMKH010000000
VERSION     DATMKH010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3423275"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="VNV63_05830"
     CDS             complement(<1..448)
                     /locus_tag="VNV63_05830"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62174.1"
                     /translation="MSSEAAPQSKQTGRPSLFRRYENLTVLAIFVGFVVIIVILQLFL
                     TGGTKFPTFISPVNLLNILQQVGVPGIVAVGMTIVMISGGIDLSVGMLASLVSIITAL
                     GISKWQLGVVPAVLLGIFSAVVLETIMGYIISRTKVEPFIITLGGMI"
     gene            complement(445..>1005)
                     /locus_tag="VNV63_05835"
     CDS             complement(445..>1005)
                     /locus_tag="VNV63_05835"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXC62175.1"
                     /translation="LINGKVVKHSTPEKAIRNKMCFITEDRQNTGLFLPQTIARNVTI
                     ANLVNTREFVVHSRDDARIGDKFVKQLAIKTPNSQTRAVELSGGNQQKVVLAKWFNTN
                     GEIFIFDEPTLGIDVGSKQEIYKVMVDLLKQGKAIIMVSSDMPEVISMSDRVLVMKNG
                     EKMAELRGDEISEENILINSIGDKTI"
BASE COUNT          239 a          265 c          253 g          248 t
ORIGIN      
        1 tgatcatccc gccaagtgtg atgatgaatg gctcaacttt cgtgcgggag atgatgtacc
       61 ccataatagt ctcaagcact accgctgaga aaataccgag gagaacggct ggcacgacgc
      121 cgagctgcca tttggagatt ccgagcgcgg tgatgatcga cactagcgac gccaacatcc
      181 cgacggacag atcgataccg ccagagatca ttacgatggt catcccgacg gctacaatgc
      241 cgggcacacc cacctgctga agaatgttca gcagattaac cggactgata aaggtaggga
      301 atttagtgcc gcccgtcagg aacagttgca ggatgacgat gatcaccacg aatccgacaa
      361 agatagcaag gacagttaaa ttctcgtatc gcctgaaaag agagggcctg cctgtctgct
      421 tcgattgggg agccgcttca ctactcatat ggtcttatct ccgatcgaat tgatgagaat
      481 attttcttcg gagatctcat caccgcggag ttccgccatc ttctctccgt tcttcatcac
      541 caggacgcgg tcgctcatcg agatgacctc aggcatatcg gaagaaacca tgatgatggc
      601 tttcccctgc ttcagcaggt cgaccattac cttgtagatc tcctgctttg agccgacgtc
      661 gatacccaag gtcggctcgt caaaaatgaa gatctcgcca ttggtgttaa accatttcgc
      721 cagcaccacc ttttgttggt tgccgccgga caattcgact gcccgggttt gcgaattcgg
      781 tgtcttaatc gcgagctgtt ttacaaattt gtcgccgata cgagcatcgt cccggctatg
      841 caccacgaat tcccgggtgt taaccaggtt cgcaatggtg acgttccgcg cgattgtctg
      901 gggaagaaag agcccggtgt tttgccgatc ttccgtgatg aaacacattt tatttcggat
      961 ggctttctcc ggggtcgaat gtttgaccac ttttccgttg atcaa
//
LOCUS       DATMKH010000490         1031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4399562, whole genome shotgun sequence.
ACCESSION   DATMKH010000490 DATMKH010000000
VERSION     DATMKH010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4399562"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(369..959)
                     /locus_tag="VNV63_05840"
     CDS             complement(369..959)
                     /locus_tag="VNV63_05840"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015412997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HXC62176.1"
                     /translation="MKPFITKDTPVTDEVLNTIAHLPTKSLPKIVDAGFFKKLSDRDF
                     MRIAFLLAKKGYEEGGSPIGGVIISDETRQILGKGHNTLVQENHPYNHGETSAVRDAG
                     RQDFSQTTMFTSLSPCDICASLIYMRQFKRLVVGDVTNSFGNEPMLRQKGVQVDILED
                     PEGIAFYAKYRKEKPDQDLEDWKGLAAVRKASAAKP"
BASE COUNT          226 a          280 c          263 g          262 t
ORIGIN      
        1 ttgtattatc gcgtccccct agagctccaa tccttgtcgg gagggtcgta tgacccagca
       61 gtccagaaac gaaaatgctc gagtcgtctg tcttttttgt ggagcccgaa cctttgttcc
      121 cgcttccgcg acccgcgata ccgctcatca gcaggagtcc ggccctggga ttgccctcat
      181 tcgctgccac gtctgctgca aagaagcacc ctattccacg agcgaaatct ttgtgcccca
      241 ggaagtctcg cttgacagca acgctcggtc tcgcgccgcc ggtttctaga cggatttggc
      301 ggcaacaatc ctgcagccat cctgcgctaa atttttagct taaggaatct gggatatctc
      361 tgggatattt aaggtttggc ggcagaggct ttgcgtacag cagcgagacc tttccaatct
      421 tcgaggtcct ggtccggctt ttctttccgg tacttcgcat agaaggcaat gccttcggga
      481 tcctccaaaa tatctacctg cactcccttt tgccgtagca taggctcatt accgaaagaa
      541 tttgtgacat ccccgacgac aagccgcttg aattggcgca tgtaaatcag ggaggcgcag
      601 atgtcgcaag gactcaggga tgtaaacatt gtcgtttggc tgaaatcctg gcgaccggca
      661 tcgcgcacgg cggacgtctc gccatgattg taaggatgat tctcctggac taacgtgttg
      721 tgccctttcc ccagaatctg gcgcgtttca tcgctgatga ttacacctcc gataggagag
      781 ccgccctcct cgtatccctt ctttgccaaa agaaaggcga tgcgcatgaa gtcccgatcc
      841 gaaagtttct tgaagaagcc ggcgtctaca attttcggaa gacttttggt cggaagatga
      901 gcaatggtgt ttaagacctc atccgtcaca ggagtatctt tggtgatgaa gggcttcata
      961 acggctcctt tcgggacccg atgcgatcag gctcagcgat cgggggattc cattctccaa
     1021 gaagaattaa a
//
LOCUS       DATMKH010000491         1434 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4399827, whole genome shotgun sequence.
ACCESSION   DATMKH010000491 DATMKH010000000
VERSION     DATMKH010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1434
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4399827"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="VNV63_05845"
     CDS             <1..610
                     /locus_tag="VNV63_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62177.1"
                     /translation="NGKELPVGGWAPGNYKLILTLTDPATNQDTYSTMDFRVGSGFAT
                     PDPWGIDDREETAKEVRNGERDYQRGLCYLAAGDNDSASPALHTALDKNPGSQRALTA
                     LVDADFLRKAYPEVAKYATSVSVTDRTEERTILQLAQSLDLTGDTKNAISVLEGSLKS
                     RKPSGPIYLTLAGYYTKQGLSDKASQYEKKGRELMLTHESQN"
     gene            complement(701..>1434)
                     /locus_tag="VNV63_05850"
     CDS             complement(701..>1434)
                     /locus_tag="VNV63_05850"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXC62178.1"
                     /translation="YRDEVKHVDLTLTPTASVNVQLVPEFGANTTTSPVSKEATIASA
                     VLAIPEGAKKEFETGQKLLDEKHDVSGSIGHFRKAIKLYEAFPQAYTMLGLAFLHEQN
                     FSESKAALERAIQLDPESGPSYIALGGCLNQMKDYPAAEKVLVKGLEILPESPDGNYE
                     LAKTYWALHRWPEAEPHAVKAKNLEPDVPGVHVLMGNIFLQRRDTDGALKEFNEYLKL
                     DPHGPMSDSVRAMVAKLEKASGTNK"
BASE COUNT          322 a          380 c          377 g          355 t
ORIGIN      
        1 caacggcaaa gagcttcctg tcggaggttg ggcaccgggg aattacaaac tcatcctcac
       61 attaacagat ccggccacta atcaggatac gtactcaacc atggatttta gagtgggatc
      121 cgggttcgca acgccggacc cttgggggat tgatgatcgg gaggagactg ctaaagaagt
      181 gcgaaacggt gaacgagact atcaacgcgg tttgtgctac ctggctgcag gggataatga
      241 ttcggccagc cccgcgctcc acaccgctct tgataaaaat cctggctctc agcgggcact
      301 aactgctttg gtggatgcgg acttcctgcg taaggcgtac cctgaggtag ctaagtatgc
      361 caccagtgtt tccgttacgg atcggaccga ggaacgtact atcttgcaac tcgcgcaaag
      421 ccttgatttg acaggggata cgaagaacgc gatcagcgtc ctggaaggct ctctcaaatc
      481 tcgcaaacct tctggtccaa tttatctcac ccttgccggc tactatacca aacagggact
      541 ttccgacaag gcttcacagt atgagaagaa gggacgggaa ctcatgctga cgcatgaatc
      601 tcagaattga tttgctgtct ccgcctgggc tgatgcactt ttcaggttcc tttgcggctg
      661 agaactctta acagaggctg aaatctgatt cgcggcgggg ttatttgttt gtgcccgaag
      721 ccttctcgag ctttgccacc atcgcgcgaa ccgaatcgct catgggaccg tggggatcaa
      781 gtttcagata ttcattgaac tctttcagag ctccgtccgt gtcacgtctc tgtagaaaaa
      841 tgttccccat cagaacgtgt acgccgggaa catctggttc gaggtttttc gcctttaccg
      901 cgtggggttc cgcttccggc caacggtgca aagcccagta tgtcttcgcc aattcgtagt
      961 tcccgtccgg ggactctggc aggatttcca gacccttgac aagcaccttt tccgcggccg
     1021 gatagtcttt catctgattt aggcaccctc ccagggctat gtaagacgga cctgattcgg
     1081 ggtcgagttg gatcgcgcgc tcgagggccg cttttgattc agaaaaattc tgttcatgga
     1141 ggaatgccaa ccccagcatc gtataagcct gaggaaaggc ctcataaagc ttaatagcct
     1201 tgcgaaagtg gccgatgcta cccgacacgt catgcttctc atccagtagt ttttgcccag
     1261 tctcgaactc tttttttgcc ccttctggga tggcgagcac tgccgatgca atcgtagctt
     1321 ccttcgaaac cggagatgta gtcgtattgg caccgaattc ggggacaagc tgcacgttaa
     1381 cggatgcggt aggcgtgagt gtcagatcaa catgctttac ctcgtccctg tacc
//
LOCUS       DATMKH010000492         1043 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4065405, whole genome shotgun sequence.
ACCESSION   DATMKH010000492 DATMKH010000000
VERSION     DATMKH010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4065405"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="VNV63_05855"
     CDS             complement(<1..326)
                     /locus_tag="VNV63_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62179.1"
                     /translation="MSWLQVEGILGDSDAWMEAAHSGSDVSLDLILADPSSEFPELYK
                     LVDVSAVHDVRVTIQARPGLLKAVKLAAALRFPIRLLPGQPKADVLAELKQALDFYLH
                     DPAVEAP"
     gene            complement(802..>1043)
                     /locus_tag="VNV63_05860"
     CDS             complement(802..>1043)
                     /locus_tag="VNV63_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62180.1"
                     /translation="YLFQGFDLGEQNFEYEFSIGELASDTGWMMTSGNVSGKKDGKDF
                     VFPVNISLSVKKDGGKWLIAAMHFSTLTDQVQNEE"
BASE COUNT          268 a          312 c          241 g          222 t
ORIGIN      
        1 ggcgcctcga ctgccggatc atgaagatag aaatccaaag cttgtttaag ctctgcgagg
       61 acgtctgcct tgggttggcc aggtaacaac cgaatcggaa atcgtaaagc ggccgcgagt
      121 ttaaccgcct tcagtaaacc cggccgggcc tgtatggtca cccgtacgtc gtgaaccgcg
      181 gaaacatcca ccagtttgta aagttcaggg aactccgaag agggatcagc aaggatcaag
      241 tcgagcgaaa catctgaacc actatgagcc gcctccatcc aggcatcgga gtcgccgagg
      301 attccctcaa cctgaagcca actcaagcta ctgggtggct cccggagcca tcggcaaagt
      361 tcatcccgac ttcggacgat gacagcaaaa ggttcatcac ctggtgtctc cgccaaagca
      421 gagggcaata tcctaatgac cggataattc tcctcggaca cgcaaataaa tccagactac
      481 cattcaccag cgattgatcc acgaacttcc ccgaggacga ccgccccacc ggttaatcca
      541 cctgccgcga ccaccacccc atcggttgat ccatccgccg cggcgaggac cccatcggtt
      601 gatccaaatt ccagcttcga cctttggcgc gaaggctaac atggcacccc caaaacccaa
      661 cgctgcaatc gcggcaccag accatttccc catactccga agaaataagc gcctgtcttg
      721 cgattccggg gatttctgct cgttctctga gccgatttca gaagatggta aactattcat
      781 aagtaagaaa ttaggagggt gttactcctc attctgaacc tgatctgtga gcgtcgaaaa
      841 atgcatggcg gcgatcagcc actttcctcc gtcttttttc acagagagcg agatgttgac
      901 aggaaataca aaatcctttc cgtccttttt gccactgacg tttccggacg tcatcatcca
      961 acccgtgtcc gacgcgagct cgccgatgct gaactcgtat tcaaaatttt gttctcccag
     1021 atcgaatccc tgaaacaagt acg
//
LOCUS       DATMKH010000493         1035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_231264,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000493 DATMKH010000000
VERSION     DATMKH010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231264"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1010)
                     /locus_tag="VNV63_05865"
     CDS             complement(<1..1010)
                     /locus_tag="VNV63_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62181.1"
                     /translation="MIRQIPPFRVFRQAAEVEQKGFPDREGWLLAIGLILGWIGLSVW
                     QAGSWRLGGWVVAAIGLSVLILLAASSAMLRLMKQISRNLPPRFPSVSGSLILRYGIG
                     NLHRSGRLIATIILSVGIGVMILLTLTQVEQSLMAQLKQNIPEDAPSFFFIDLQPDQK
                     EPFVAMMKRWEFKKPPQLTPLVRSRLYDVKGEKVSEMKTENRPDGWYFTREYVLTSQK
                     DLPEHNSLQRGKWWPETAGQSGSKNTPLISVEAEAARHLGIDLGDTVTFDIQGKLISA
                     VVANIRDVDWGSLSVNFFFIFSPGAFEGAPITYVATVTTRPEEDLPLQNAVIGAFPNV
                     TA"
BASE COUNT          244 a          283 c          284 g          224 t
ORIGIN      
        1 atcgccgtca cgttcggaaa ggctcctatg accgcgttct gaagcgggag atcttcttcc
       61 ggccgtgtgg tgacggtcgc tacgtaggtg atcggcgctc cctcgaaggc gccgggggag
      121 aagataaaaa agaaatttac cgaaagactg ccccagtcca cgtcacggat gttcgcgaca
      181 acggcggaga tcagttttcc ctgtatgtcg aatgtcaccg tatcgccgag atcaattcca
      241 agatgccgcg ccgcctcggc ctcgaccgag attaatgggg tgttcttgga accgctctgt
      301 cccgctgtct ccggccacca ttttccccgt tggagagaat tatgttcggg taaatccttc
      361 tgagaggtta ggacgtattc gcgtgtaaag taccaaccgt cgggacggtt ttctgtcttc
      421 atctccgaca ctttttcccc cttaacatcg tacagccgtg accgaacgag cggtgtgagc
      481 tgcggtggtt ttttaaattc ccatcgtttc atcattgcta caaacggttc cttctgatca
      541 ggctggagat caatgaaaaa aaagcttggg gcatcttccg gaatattctg tttcagttgt
      601 gccatgaggc tctgttcgac ctgggtgagc gtgagaagga tcatcacacc gatcccgacc
      661 gaaagaatga tcgtcgcgat cagccgtccg gaccgatgga gattgccgat cccgtagcgc
      721 aagatcagtg atccggaaac agagggaaag cggggtggaa gattacggct gatctgtttc
      781 attaaacgaa gcatggcgga tgaggccgcg agcagaataa gtacagataa cccaatggcg
      841 gccacgaccc atcctcccag ccgccacgaa ccggcctgcc agaccgacag gccgatccag
      901 ccgagaatca atccgatcgc gaggagccag ccttctcgat cgggaaaacc tttctgctcg
      961 acctccgccg cttgtcggaa aactcgaaac ggcgggattt gccgaatcac cctcagaggc
     1021 cacagagaga agaga
//
LOCUS       DATMKH010000494         5199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2467830, whole genome shotgun sequence.
ACCESSION   DATMKH010000494 DATMKH010000000
VERSION     DATMKH010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 5199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5199
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2467830"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..320)
                     /locus_tag="VNV63_05870"
     CDS             complement(123..320)
                     /locus_tag="VNV63_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62182.1"
                     /translation="MMTGEKLVAAQAAMKARPKRKRNRRYCVPGRKRLELLNMRALTP
                     EEKIQWYRENDPRCQMVKPHE"
     gene            complement(317..631)
                     /locus_tag="VNV63_05875"
     CDS             complement(317..631)
                     /locus_tag="VNV63_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62183.1"
                     /translation="MNQTDQRAYWLWQYRKKEAKRKGLPFTEPKPVSTAKLTSAPKAP
                     PRFIDQKPCRTKALLWKKRSLEIKRELLSKSVFYDGFEADVAANNQQLRDVHDLLRQA
                     AA"
     gene            complement(636..1157)
                     /locus_tag="VNV63_05880"
     CDS             complement(636..1157)
                     /locus_tag="VNV63_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62184.1"
                     /translation="MNIFSRKTSAPSVAEVPVQVDPFNDPDDERCSNPRCSHFISAKH
                     TPNAVVYCGRTFCEESCVPPSLRERDRIEKQQDRAFREKTQADAIALAKANRILTLRQ
                     QIAECKHFLEVAPGSAIATAQLREFEIALAAELEIGNTDPHHVIVPDRNQIVEHHGRG
                     FTSFTGDSFHGEI"
     gene            complement(1257..1469)
                     /locus_tag="VNV63_05885"
     CDS             complement(1257..1469)
                     /locus_tag="VNV63_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62185.1"
                     /translation="MGKLLIEKFIAGDPIDVSKLTYQDRCSLTYDLCVLQAGQERRNI
                     AKAAGESKSVFEQRIAVMLKRLQAGK"
     gene            2248..2556
                     /locus_tag="VNV63_05890"
     CDS             2248..2556
                     /locus_tag="VNV63_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62186.1"
                     /translation="MKRSELKKRQREMKSRNVALRAAYKMPLDALFRERFFKLCRSCG
                     WDEDRTQRAFFAISFGPWIQDIRHDLRMLDYLRAEFGGALPHYDALRGMTSNFPRPVA
                     "
     gene            3466..3975
                     /locus_tag="VNV63_05895"
     CDS             3466..3975
                     /locus_tag="VNV63_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62187.1"
                     /translation="MNSPKIEPREQLIARIRSNFPTELIGHPGKPQAEKWCVWKYVER
                     SGKRTKLPFQPNGDPAESNNKNTWSTFDAVSEVFLAGKFDGIGCFISEPYVAVDFDHV
                     CKDFDPSTIETWVLEAVSRLGSYTEFSPSGEGLHVWSYGRLPGKGKRAGRIEIYGEGR
                     YFTVTGIRL"
     gene            3996..>5199
                     /locus_tag="VNV63_05900"
     CDS             3996..>5199
                     /locus_tag="VNV63_05900"
                     /inference="COORDINATES: protein motif:HMM:NF024872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXC62188.1"
                     /translation="MNEISSEFYASVETLDPQHKKPVASVKDASTNKISAESRFLRLF
                     AGDFEGLQPSASEAVHEVLIRLAQRHSNNPVLMREDFEHSGLYHRTDWKNKWHRLAES
                     EIEKAVAKNPNPKKLSGYTGDGSDWREGCLSKDQLSAQLPKFLLGGLIPEKCLTFITG
                     PSYHCKTWLAVQSGHAVSTGANLWCFDGPGEVVPVIYHVPEMNAAFVRQRMDQVGIEN
                     SELFLVRPMETGIWALDDPRMLKSSEGRLVYLDTQGFFNPADDASDYKQALKFGQLVF
                     NLLTNGARGVVALAHPPKYATQSKNEPAWTLENSVLGSAGYGGILRSCLRVRNLNLDL
                     NDPHVWLYVQGLKNPGLKPFQLEGLPLKMRVPPGESPYLGILLESIEGTVDPRKLKAF
                     EMFSKGVSR"
BASE COUNT         1160 a         1431 c         1384 g         1224 t
ORIGIN      
        1 tttctccgga ctcgccaacg gttttgaggg ctccactgag ctgatcggcc gagctggaca
       61 cttccttgat cgaagctgtt gctcctgaag catcccccgt gattgctatt cggatctgat
      121 cgtcactcat gaggtttcac catctggcat cgcggatcat tctcccgata ccactgaatt
      181 ttttcttccg gagtcaaagc cctcatgttg aggagttcga gccgcttccg tcccggcaca
      241 caatatctac ggttccgctt acgcttcggc cttgctttca tagccgcttg agcggcaacg
      301 agtttttctc cagtcatcat gcggccgctt gtctcagaag atcgtggaca tcgcggagct
      361 gttgattgtt cgcagcaaca tcggcctcga atccgtcata gaagacgctc ttcgagagca
      421 actcccgctt gatctccaga gaacgcttct tccagaggag agctttagtg cggcaaggct
      481 tctggtcaat gaagcgggga ggagccttcg gagctgaagt cagcttagct gtggacacgg
      541 gcttcggctc ggtgaacggc aatcccttcc gcttcgcttc cttcttgcgg tactgccaaa
      601 gccaataggc tctctgatcg gtttgattca tgagatcaga tctctccgtg gaatgagtcc
      661 ccggtgaagc tcgtgaagcc tcggccatga tgttcaacga tctgattccg atccggcacg
      721 attacatggt gcggatccgt attcccaatc tccagctcgg ccgcgagcgc aatctcgaac
      781 tctcggagtt gagctgtagc aattgcggaa cctggagcca cttcaaggaa gtgcttgcac
      841 tcggcaatct gttgccggag cgtgaggatc cggtttgcct tcgcaagtgc gatagcatcg
      901 gcctgagtct tctcacggaa agctctgtcc tgttgcttct cgatgcggtc ccgctcgcgc
      961 aagctcgggg gaacgcaaga ttcctcacaa aaagttcggc cgcaatacac cacggcattt
     1021 ggggtgtgct tcgcgctgat gaagtgactg catcgcggat tcgagcaacg ctcatcgtcc
     1081 ggatcgttga acggatccac ctggacggga acctcggcca cacttggagc ggaagtcttg
     1141 cgtgaaaaga tgttcatgaa tttttctcct tggattttga ccgggctcgg tcggaggacc
     1201 gagccggacg attcagcacc cgcagggaga aacctaacgg gtgtaatcga gaacttttat
     1261 ttcccggcct ggagccgttt cagcatcaca gcaatgcgtt gctcgaacac ggacttggat
     1321 tctccagcgg ccttcgcaat gtttcgacgc tcttgccctg cctggagaac gcatagatcg
     1381 taggtgagcg agcagcggtc ctgataggtg agcttgctca cgtcaatcgg atccccggca
     1441 atgaattttt cgatcagcag ttttcccata tatcacctcg tggtgtcatt tggtagagcc
     1501 ggataacccg gagtcttgtt cggtgcatct ggagcgaacc ccgtggagcg cggcacagat
     1561 ggaggcatgt ccgattcccg tggcaaacct ccaggagccg gagccgcttc gttcgcagcc
     1621 ggaggattgt tgaacctcga cgcgatgtcc cgctgaaaat ctgtgacgat gcggtacgtc
     1681 attttccaat ccctgagttc actccatcac tcgatccccc gttgccggga gtgcgaaccg
     1741 tggaatgccg gaagtccggg acggagctga ctccacctcc gtatggattg ggaacgggag
     1801 attccgagcc cacacggctc gaaggcccac cactcgtaat gtttgcttcc gttgcttgat
     1861 ctgccataag tcctccaaaa agttgaagcc gggtgagtgt gatgcccagg agtaaggcca
     1921 cccggcctct cttcatgact cttcaaggct aatacagcct ctccgctcct gtcagaatcc
     1981 ggaaacaata cccaatgttt atgaacctct agggtggaac tccctatgct ataattggga
     2041 tatgccgtac tcaattgaac ccgatcagat ccggatccgc ttcaagaagc gggacgtgga
     2101 gttgtacgaa acactccaag ctatgtcaga agaaacgaag ctatctctct ccgaagttgc
     2161 gcggagattg ctggacttca cggtccacag gattgagcag ttaccagcga agcaacggca
     2221 agtgtttttg aagcagtggg gaagatcatg aagagatcag aactgaagaa gcgtcaacgc
     2281 gagatgaagt cccgcaacgt agctctcaga gccgcgtaca agatgccgct ggacgcgctc
     2341 ttccgcgagc gattcttcaa gctgtgccga tcatgcggat gggacgagga ccggacacag
     2401 agagcgttct ttgccatctc cttcggcccg tggatccagg acatccggca cgatctccgg
     2461 atgctggatt acctgagagc cgaatttggg ggagcgttgc cgcactatga cgctctacgc
     2521 ggcatgacga gcaactttcc ccggcccgtt gcgtgacgca tgattgcttc acaggcttct
     2581 tgccgctcct gaagagatag atccctcagc ttcttcccgc tcacgatcat cgcgcaggct
     2641 tcctcagctt gctctcgcgt tatcggtccc gcttcctcga ttgcttgctc ccaaaggttt
     2701 gtcatttgtt tccctccagc accatcatcc ctcacgagcc tcagtgctcc aagattccaa
     2761 tcgagctatt actttaagaa actaaagtgt gacagctatt tcctatgaat ttattgtcaa
     2821 gttgacaaag agtgggccac ctttttctcg aatttttact ttttgggatc caggagccgg
     2881 gactcgaatt tttcagttgg gtgcggaggt gcgtaatagg cttttcggat caaacttcgg
     2941 ttgtaatggg ccgggtggct tgcctcacgg ctctcataac aaaagactta gtgacgatca
     3001 ggaatcatgg aattgattcc atgagctgta aactgctgat aacaatcata gtgtcatagg
     3061 ttacagacct atcgcactcg attttcgaat agcggtaagc cttgcgacgc cgtccgttcc
     3121 cgttccctca gctaagtaaa agaatgggaa cgacacaaga aaggcttatc ttgccttctt
     3181 gtgatcgtgt ttcgtacccc ggttcccaac tgtgggaacg gagggaacgg attctgaatc
     3241 ccgcgtcctg atgttcgcct tgagttcgcc cttataagaa gctatagaag tcaacaatat
     3301 gttataagtc tatatttatc aataacttac agttgcaata gctgctttgc tctgttatcc
     3361 ttagaaagtc aactgagaca ccttccggca tgatccccgg acaggcattg cttgagacgg
     3421 gttggagtcc acgcctccaa cccactcagc gcgtggagca agacaatgaa ctctcccaaa
     3481 atcgaacccc gcgagcagct cattgcacgc atccgctcaa acttcccaac agaactgatc
     3541 ggccatcctg gaaaaccgca agctgagaag tggtgcgtct ggaaatacgt ggagagatcc
     3601 ggcaaacgca ccaagctgcc atttcaaccg aacggagatc cggccgaatc gaacaacaaa
     3661 aacacctgga gcacgttcga tgcggtaagt gaagttttcc ttgcgggaaa gttcgatgga
     3721 atcggttgct tcatctctga gccgtatgtt gccgtggact tcgatcacgt ctgcaaggac
     3781 ttcgatcctt caaccattga gacttgggtg ctcgaagccg tgtcccggct cggcagttac
     3841 accgagttct caccttcggg agaaggcttg cacgtctgga gctatggaag acttcccggc
     3901 aaaggcaagc gagcgggacg aatcgaaatt tacggggaag gacgatactt caccgtcacg
     3961 ggcattcgcc tatgagtccc ctcactccaa gagcggtgaa cgaaatctca tccgagttct
     4021 atgcgagcgt tgagacactc gatccgcaac acaagaaacc cgttgcgtcc gtgaaggacg
     4081 cgagcaccaa caagatctcc gctgagtcca ggttcctccg gttgttcgcg ggagacttcg
     4141 agggattgca accatcggcc tccgaggccg tgcatgaagt tctgatccgg ctcgctcagc
     4201 ggcacagcaa caatcctgtg ctgatgcgcg aagacttcga gcacagtggg ttgtatcacc
     4261 ggacggattg gaagaacaag tggcatcggc tcgcggagtc cgagatcgag aaagctgttg
     4321 caaagaatcc gaatcccaag aagctctccg ggtacaccgg ggacggatcc gattggcgag
     4381 aaggttgtct ctcgaaagat cagctctcgg ctcagcttcc gaagttcctg cttggtggtc
     4441 tgattccgga gaagtgcctc accttcatca ctggaccgtc ctatcactgc aagacgtggt
     4501 tggccgttca atccggccac gctgtctcca caggagcaaa tctgtggtgc ttcgatgggc
     4561 cgggtgaagt tgtgccagtg atctaccacg tccccgaaat gaatgcggct tttgtgcgtc
     4621 aacgcatgga tcaggttggg atcgagaact ccgaactatt tcttgtgcgg ccgatggaaa
     4681 caggcatttg ggctctcgat gatccccgta tgttgaagtc ctcggaagga aggcttgtgt
     4741 acctggacac gcaaggcttc ttcaatccgg ccgatgatgc ctccgattac aagcaagctc
     4801 tgaagttcgg acagttggta ttcaatctcc tcaccaacgg tgcgcggggt gtggttgcgc
     4861 tcgctcaccc tccgaagtat gcaacccaat cgaagaacga acctgcctgg acgctggaaa
     4921 acagcgtgct tggctcggcc ggatacggag gaatcctccg ctcctgtctc cgcgtccgca
     4981 acctgaatct cgatctcaac gatccccacg tgtggttgta cgtccagggc ttgaaaaatc
     5041 ctggactgaa acccttccag ctcgaaggac tgccgctcaa gatgagagtt cctcccggtg
     5101 agtccccata tctcggcatc cttcttgaat ccatcgaagg caccgtggat ccgcgcaagc
     5161 tcaaagcctt cgagatgttc tccaagggag tgagccggg
//
LOCUS       DATMKH010000495         1327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4066345, whole genome shotgun sequence.
ACCESSION   DATMKH010000495 DATMKH010000000
VERSION     DATMKH010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1327
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4066345"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(564..1022)
                     /locus_tag="VNV63_05905"
     CDS             complement(564..1022)
                     /locus_tag="VNV63_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018969745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HXC62189.1"
                     /translation="MSSPRYRSNVAAIIQAADQKILIGERSDVPDAWQFPQGGVRKGE
                     SALEALFRELREEVSLKPSHYRVLDSKGPYRYLFQDGRKKEGFDGQEQFYFLVRLTAP
                     GSYVKVETDNPEFSRIRWIEPIDFRLQWLPEFKREVYRQVFLDFFGIRPA"
     gene            complement(1025..>1327)
                     /locus_tag="VNV63_05910"
     CDS             complement(1025..>1327)
                     /locus_tag="VNV63_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62190.1"
                     /translation="YQDQIRVEGAGSIGPDHVDADLVVGLSPKILGWIPGAEEKVFTD
                     QKDGLHWTEVRISGTPEQPKEDLTKRLVSAFRDKMTKEFKGQAKDAIKSLLDMLHR"
BASE COUNT          337 a          374 c          323 g          293 t
ORIGIN      
        1 gaccggagtt ccaaagccgc acaccgcaaa acgcggtcgc ctcggagatg cgcccctagc
       61 ttttgcgtcc gaccaattgc aggccatcga actgagaatc ctcgcgcacc gcgtcaaaac
      121 cgacagataa acgctagtgg cacaaaccga agaagacaga attcactctg ttctgcctgg
      181 atttagcccc gttttctccc gagccgcttc ctgagcggct ccagagcctg gaaatctcac
      241 gagcgctctg gctcgtgaaa tttccaggct aacgccaaac cccgacctca tcggctgatg
      301 aagtcagaca cggggaagta atcctggccg cagcacaggt tactattgac taacatgatg
      361 gaatcgtccg gcgacgaccg gtcgacagaa aatcagcgtg aacggttcac gtcatctttt
      421 acgcacaccc accaaacaca ccccaggagt gcggcaccaa gaaacaaaaa cattcccaat
      481 gtcaacatac gccactggtg atgccggaat acagggtgag agtcaaaggg aaattacagc
      541 atccagaaaa aagcttatct ggctcaggct gggcgaattc caaaaaaatc caggaaaacc
      601 tgtcgataaa cctcccgctt gaattccggt agccactgga gacggaaatc aatgggttca
      661 atccagcgaa tccggctgaa ctcgggattg tccgtctcta ctttcacata ggagcccggg
      721 gccgttagcc ggacaagaaa ataaaattgc tcctggccgt cgaacccttc ctttttgcgt
      781 ccatcttgga aaaggtaacg atagggaccc ttagagtcca ggacacgata gtgggaaggc
      841 ttgagagaaa cctcttcccg cagttctcta aacaatgcct ccaaggcgga ctcgcctttt
      901 ctgacgcctc cctgggggaa ctgccaggcg tcaggcacgt ccgaacgttc gccgatgagg
      961 attttctgat ctgctgcctg aatgattgcc gccacattcg aacggtagcg aggtgaagac
     1021 atgattacct gtgaagcata tcgagcagag acttgatagc atcttttgcc tggcccttga
     1081 attcctttgt catcttgtcc ctgaaggcgc taaccagtcg tttggtcaaa tcttcttttg
     1141 gttgttcggg cgttccagat atccgtactt cggtccagtg caaaccatct ttctggtctg
     1201 taaagacctt ttcttctgcc ccaggaatcc accctaggat tttgggagaa agaccaacaa
     1261 caaggtccgc gtcgacgtgg tcgggtccta tcgatcccgc gccttcgacg cgtatttgat
     1321 cctgata
//
LOCUS       DATMKH010000496         1176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2133444, whole genome shotgun sequence.
ACCESSION   DATMKH010000496 DATMKH010000000
VERSION     DATMKH010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2133444"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            456..1085
                     /locus_tag="VNV63_05915"
     CDS             456..1085
                     /locus_tag="VNV63_05915"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXC62191.1"
                     /translation="MRNKDRRPAGFVVLFVVFGLLKIGSAEARNLVPEELQYLEEPDR
                     GEWQMPERVIEALRLRESDVVADVGAGTGYFSRRFARQVAHVYAEDVDPEALSFLRRE
                     ALAKVTVVPGKPENPMLPPNSCNLVFICDVLHIVENRPAFLRNIIPALKPGGKVAVIE
                     FYRRELPVGPPLWAKLSEEEAKKDFLKGAFKLDKQLTFLPYQYFLIFTK"
BASE COUNT          287 a          311 c          292 g          286 t
ORIGIN      
        1 cagccgtagt tgagacaatc gcctcccatc ttatttcgct cgactagagc gacacgtccg
       61 ccgagcccag cggttcccgc ggcgactacc agacctgcgg tgccggcccc gatgatcacc
      121 acattataaa cgcccaaacc ttttttttct tgcataatga gcaactcgga ccagacgcgt
      181 ctccgtggat gacttcgtga cgccgcttat tcccgatttc ttttttcttc cggtcacgat
      241 gcgcgtctta ctgcatcctt gatacgctgc cggccgttct cgaaagagaa gcttctgata
      301 tcctctcgcg gttctccggg gagccttccc ttccaatgga agccctattc gtttcaaagg
      361 caaaagagca aaagcaaaaa ggccagcatc accgcgaagg atcgcaaaaa gccggatgtt
      421 tgattctcaa tcgtcctcgt cgttattgta aaaaaatgag aaacaaagat cggagaccgg
      481 cgggatttgt ggtgctgttt gtagtgttcg gactcctcaa aattggttcg gcggaggctc
      541 gcaacctggt tccggaagaa ctccaatacc ttgaggaacc agatcgtggt gagtggcaaa
      601 tgcccgagcg cgtaatcgag gcattaagac ttcgggaaag cgacgtcgtc gcggatgttg
      661 gtgccggcac aggatatttt tcgcgtcgat tcgcacggca ggtagcgcat gtttacgcgg
      721 aagatgtcga tcctgaggct ctgagttttc tccggagaga ggcgctcgcc aaggtaactg
      781 tcgttccggg taaacctgaa aatccaatgt taccgccgaa ttcgtgtaac ctggtcttta
      841 tatgcgatgt tctccatata gtcgaaaacc gtcctgcctt cctacgtaac attatccccg
      901 ctctgaaacc aggcgggaag gtggccgtaa tcgaatttta cagaagagaa cttccggtcg
      961 gaccccctct gtgggcgaaa ctatcggagg aagaagcgaa gaaagacttt ctgaagggag
     1021 cctttaagct ggacaaacaa ctaacctttc ttccctacca gtacttcctg attttcacaa
     1081 agtgaccgag tcatcgcttc tttcgccaga cgtgatcgaa cgctacgccg agaatttcga
     1141 tcaaggtcaa agaagatcag tttcatcaaa gttcta
//
LOCUS       DATMKH010000497         1144 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2468595, whole genome shotgun sequence.
ACCESSION   DATMKH010000497 DATMKH010000000
VERSION     DATMKH010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2468595"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..919
                     /locus_tag="VNV63_05920"
     CDS             <1..919
                     /locus_tag="VNV63_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62192.1"
                     /translation="EEEEEEKQDQPGKRSAGTYADGHPLDQIQYLEAKLILKPDRFTS
                     VESFREFGKLVKKTAKKEDVGFVPDVEAGLRPNIREILFLDTPDFRFYNNAFILRRRI
                     NYVDGFPVGDPEIVFKYRHPDEKTAAAVDVRPKISGKYRIKFKAEALPLKDRIGSYRV
                     LYSHNCVFGLSQVHEGDRTSMQTLVRVFPALEMLKKSPDERVTLVSEAIVEEVLLKLG
                     ELDFGKGQVGKCDVALWRTRGEHQSLVGEFAFQVKFDSREDVHQKAKKRCEQFFISLQ
                     NDVKDWISLGTTKTGMVYRLKGNAPQSHE"
     gene            912..>1144
                     /locus_tag="VNV63_05925"
     CDS             912..>1144
                     /locus_tag="VNV63_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HXC62193.1"
                     /translation="MSETDTKLALPRGADTGAVQAGVSIGQIFLEFLIIGATSFGGVV
                     PYLRESLVIKRHWIDDKEFVEMLSISQSLPGLNA"
BASE COUNT          291 a          276 c          325 g          252 t
ORIGIN      
        1 ggaagaggaa gaagaggaaa aacaggatca gccaggtaaa aggtccgcgg gcacctatgc
       61 cgatggtcat cctctcgatc agatacagta tctcgaagca aaactgattt tgaagccgga
      121 ccgctttacg tctgtggaaa gtttccggga atttgggaag ctggttaaga agactgcaaa
      181 aaaagaagac gtcggattcg ttccagatgt ggaggccggt ttgcggccta atattcggga
      241 gattctattc ctggataccc cggacttccg tttctacaac aacgccttca ttcttcgccg
      301 acggatcaat tatgtggatg gttttcctgt gggcgaccct gagatcgttt tcaaatatcg
      361 tcatccggat gagaagacag cagcagccgt agatgttcgc ccgaagatat ctggaaaata
      421 tcggattaaa tttaaagccg aggcgctgcc attgaaggat cgcattggct cctaccgagt
      481 tctctattct cacaattgtg tgttcgggtt gagccaagtg cacgaagggg accgcacctc
      541 gatgcagacg ctggtgcgcg tcttcccggc cctcgaaatg ttgaagaaat cgcctgacga
      601 aagagtgacc ctggtgagtg aggccatcgt cgaagaggtg ctgctcaaat tgggcgaact
      661 ggacttcggc aaggggcagg tggggaagtg cgacgtggcc ctgtggcgca cacgcggcga
      721 acaccaatcc cttgtggggg aatttgcgtt tcaagtgaaa ttcgattccc gggaagacgt
      781 ccaccagaag gccaagaagc gctgcgaaca atttttcatt tcgctccaga acgatgtaaa
      841 agactggatt tctctcggca cgacgaagac aggcatggtg taccgcctca aggggaatgc
      901 accgcaaagc catgagtgag acggacacga aacttgctct gccacgagga gctgatactg
      961 gtgcagtgca ggccggagtc tcgattggcc aaatctttct cgaattcctg atcatcggag
     1021 ccaccagctt cggaggggta gtgccctacc tccgcgaaag cctagtaatc aagcgacact
     1081 ggattgatga caaggaattt gtcgagatgc tctccatcag ccagagcctt cctggcttga
     1141 acgc
//
LOCUS       DATMKH010000498         1033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1196211, whole genome shotgun sequence.
ACCESSION   DATMKH010000498 DATMKH010000000
VERSION     DATMKH010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1196211"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..>1033
                     /locus_tag="VNV63_05930"
     CDS             14..>1033
                     /locus_tag="VNV63_05930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HXC62194.1"
                     /translation="MRRIFGLDPQSSLPDREEFLRLVHPEDRDRFNQRIEKSRREKSD
                     FVQDYRVILTDGTVKYIHGIGHPVLDEAGDIIQYVGTDVDVTERKRVEEKLRHTESDL
                     LEAQRISQTGSWKLDASSGTVTVSPQIFRIFGVSPDEDTSTPEFWFSRNHIEDRKRIQ
                     ELFERSRTQKTDYEADYRIVLPDGTIKHLHAVGHPVLNKSGDLVEFVGTAMDITERKQ
                     KEEALRRSESYLAEAQKLTHTGSWAVRVPQMENVQGEAGQGLAVIPRLGWDASYWSKE
                     MYRIFGIDPGPTPPSYMEVVSRFHPEDAHYNTAVVEQAVRDRTDFEIDYRLLLPNGAA
                     KYIHVV"
BASE COUNT          272 a          256 c          289 g          216 t
ORIGIN      
        1 ctggtccgac gaaatgcgac gaatctttgg attagatcct caaagcagcc ttccggatcg
       61 cgaagagttc cttcgactgg tacaccctga agatcgcgac agatttaacc aacggattga
      121 gaagtcacgc cgcgagaaat ccgattttgt gcaagattac agggtcatat tgactgatgg
      181 cacagtgaag tacatccacg ggatcggaca cccggttctc gatgaagccg gagatattat
      241 ccagtacgtt ggtactgacg tggatgtgac agaacgcaaa cgcgtcgaag agaaactgcg
      301 ccacactgag tcagatttgc ttgaggcgca gagaataagc caaacaggca gctggaaact
      361 cgatgcttca tcagggacag tcactgtctc accgcaaata tttcgaatat tcggcgtcag
      421 ccccgatgag gatacgtcga cccctgaatt ctggtttagc agaaatcata tcgaggaccg
      481 gaagcgcatt caggaactct ttgaaagatc cagaacccag aaaacagact atgaagcgga
      541 ctatcgcatt gttcttcccg atggaaccat caagcacctc catgctgtcg ggcatcctgt
      601 cttgaacaag tcgggcgatc tcgtggaatt tgttgggacc gcgatggata tcaccgagcg
      661 caagcagaag gaagaggccc tgcggcggag tgagagttat ttagcggagg cacagaaatt
      721 gacccacacg ggcagttggg cggtacgggt ccctcagatg gaaaacgtgc agggtgaggc
      781 cggtcaaggg cttgccgtga tcccaaggct cgggtgggac gcttcctatt ggtcaaaaga
      841 gatgtaccgg atcttcggca tcgatcctgg tccaacgcca ccatcttaca tggaggtagt
      901 cagccggttc cacccggagg acgcgcacta taacacagct gtggtcgaac aggccgttcg
      961 ggacagaact gactttgaga tcgactatag gcttcttctg ccaaatggcg cggccaagta
     1021 cattcatgtg gtc
//
LOCUS       DATMKH010000499         2133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4067566, whole genome shotgun sequence.
ACCESSION   DATMKH010000499 DATMKH010000000
VERSION     DATMKH010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4067566"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="VNV63_05935"
     CDS             <1..480
                     /locus_tag="VNV63_05935"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HXC62195.1"
                     /translation="FKDEEVSPIIPAHIEKGFKFFVDQTKVTGRWDRIDEEAGKAKVI
                     DYKSSNVTTQKKADEQTGKSLQLSIYALAYREQHGAVPDSVELHFLDTGLIGRAKKTD
                     KDLEKTLTQIREVADGIRAGDFTAMPSYMACQYCAYNQICPYTGFGKGKAGGGASET"
     gene            477..1622
                     /locus_tag="VNV63_05940"
     CDS             477..1622
                     /locus_tag="VNV63_05940"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HXC62196.1"
                     /translation="MIYRTSCFLFVLSLLTACSTHSHWEEIKTARKTFEAQRLEPSPL
                     PGYHDFRGAIHVHSYISHDSKGTPDEILQAAKIARLNFLIMTDHNNPRIYQEGMNGRY
                     GNLLVIRGAEIGMNHSYILAIGLPRDLDPPPHTMEEALRQVKALGGMAIVAHPKRFRS
                     WDSQEYQAMEIYNLLDSIQEQRWRYPRYLFDFLYSFDSHPEELFLSVIERPDWGLNKW
                     DEINRDRKITAIGTSDAHQNVKFLGRRLDPYSISLRFVDTHILASRLDQEKVLSALRS
                     GHAYVSHDLLADPTGFQFFAADRTHTWIMGDDVPLGPGLQLNVKSPLSARIRLFKNGE
                     IIRETVSDVLALPVPTDGMGIYRAELSLKVLGKWWPWIFSNPIYIKG"
     gene            1663..>2133
                     /locus_tag="VNV63_05945"
     CDS             1663..>2133
                     /locus_tag="VNV63_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62197.1"
                     /translation="MSQTKKKRLFLILIIICLSFSGCITYDAPPDADPSKNGSNPVLS
                     HDFDYTHDPIPPQVGIPKSEGRFTVTEVVFHTNRQSTQPDLKNPLIPTRFYQPTVPGP
                     RPAVVILPVTRGDYPTKAVAAYLADRGIASLLFMSRSTFTGTAGKDFSTLAAQFH"
BASE COUNT          520 a          595 c          535 g          483 t
ORIGIN      
        1 tttaaagatg aagaggtctc cccaataata ccggctcata tcgagaaggg tttcaagttc
       61 ttcgtagacc agaccaaggt caccggccgt tgggatcgga ttgacgagga ggctgggaag
      121 gccaaggtca ttgattacaa atcttcgaat gtgacaaccc agaaaaaagc ggatgaacag
      181 accgggaaga gcctgcagct ttcaatctat gcgttggctt atcgggaaca gcacggagca
      241 gtcccggaca gtgtcgaact tcacttttta gacaccggtc tgatcggccg ggcaaagaaa
      301 accgataaag atcttgaaaa aaccctcacc caaatccgcg aagtggccga cgggatccgg
      361 gccggtgatt tcacggcaat gccgtcatac atggcctgcc agtactgcgc ctataatcag
      421 atctgcccct acaccggctt tggcaaggga aaagcaggag gcggagcatc agagacatga
      481 tctatcgtac cagctgcttt ttattcgtct taagcttgct caccgcctgc agtacgcaca
      541 gccactggga ggagataaag acagcccgga aaactttcga ggcccagcgt cttgagccct
      601 ctcccctgcc cggctatcac gatttccgcg gcgcgattca tgtccattct tacatctcgc
      661 atgacagcaa gggaacaccc gatgagatct tacaagccgc gaagatcgcc cggctcaact
      721 ttctgatcat gacggatcac aacaaccctc ggatctatca ggaagggatg aatggccggt
      781 acgggaatct gctggtcatc cgcggagccg agatcggcat gaaccactcc tatatactgg
      841 caatcggcct gccacgggat ctggacccgc ctcctcatac aatggaggaa gcactcaggc
      901 aagtgaaggc cctgggcggc atggcgatcg tggcgcatcc gaaaaggttt cgatcctggg
      961 actctcagga atatcaagct atggagattt acaatctcct ggattcaatc caagagcagc
     1021 gatggcgcta cccacgctat cttttcgatt tcctctactc gtttgattcc catccggaag
     1081 aactttttct ctcggttatc gaacggccgg actggggtct taataaatgg gacgagatca
     1141 accgggaccg caagatcacc gcgatcggca ccagcgatgc ccatcagaat gtgaaatttc
     1201 tcggccgccg gttagaccct tactccatca gcctccggtt cgtggacacg catattcttg
     1261 cctcccgctt agatcaagag aaggttctct ccgcacttcg aagcggccat gcctatgtct
     1321 cgcacgacct tcttgcggac ccaaccggat ttcagttttt tgcggcggac cggacccata
     1381 catggatcat gggagatgac gttcctcttg gacccggtct acaactcaac gtcaagagtc
     1441 cgctttcggc tcgaataagg ctgttcaaga acggtgagat aatccgtgaa accgtaagtg
     1501 acgtgctcgc gcttccagta ccaactgatg ggatggggat ttatcgagct gagctgtcgc
     1561 tcaaggtact gggtaagtgg tggccctgga ttttcagtaa cccgatctat atcaagggct
     1621 agcgttgaga ggacccggtg agtatggtat actcctcgct caatgagtca aaccaagaaa
     1681 aaacggcttt tcctgatcct catcataata tgtctgtctt tttcaggttg tatcacctac
     1741 gatgccccac cggatgccga tccgtcaaaa aatggatcaa acccggtact gtcgcacgat
     1801 tttgattata cacacgatcc aataccgcct caggtcggca tcccaaaatc agaaggacgt
     1861 ttcacggtca ccgaggtcgt gtttcatacc aatcgtcaaa gcactcagcc cgatctaaag
     1921 aatccgttga tccctacccg attctaccaa ccgaccgtcc cgggtcctcg gcctgccgtg
     1981 gtgattcttc cagtcacgcg cggggattat ccgacaaagg cggtcgccgc ttatctggca
     2041 gaccgtggaa tcgcatctct tctgttcatg agccgctcta cttttaccgg cactgccggg
     2101 aaggatttta gcacgctggc cgcccagttt cat
//
LOCUS       DATMKH010000500         1214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3426353, whole genome shotgun sequence.
ACCESSION   DATMKH010000500 DATMKH010000000
VERSION     DATMKH010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3426353"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..339)
                     /locus_tag="VNV63_05950"
     CDS             complement(1..339)
                     /locus_tag="VNV63_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62198.1"
                     /translation="MSEDSLTIKDYYARVTLQRHHIGNPPECDERIEVVVRIPEEVAK
                     NLAGGGKPEVVIFDVGSRVRNVATETAGVPNDSSFVIAVVSTVDAVMPPSLPTESNFK
                     DQDGAEGWIL"
     gene            338..745
                     /locus_tag="VNV63_05955"
     CDS             338..745
                     /locus_tag="VNV63_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62199.1"
                     /translation="MTGIMGSLLVRSNTEQAGHPPGFGVEMRPMSITPQDLQKLLDER
                     EAHRAPWKRARENLQEIRWVLKDVADVDLPPPARKTIDLEGRLVKDGVRTALSDRQDA
                     LRDLVEAIRKYRKLAEKTPLTLQGRILRKLSRN"
BASE COUNT          284 a          324 c          306 g          300 t
ORIGIN      
        1 ttacaagatc cagccctcgg ctccgtcttg atctttgaag tttgattccg ttggaaggct
       61 cgggggcata accgcatcca ccgtagaaac aactgcgatt acgaaggatg aatcatttgg
      121 aactcccgcc gtttcggtcg ccacgttcct aacccgcgaa cctacatcaa aaataaccac
      181 ttctggtttt cctccccccg ctaaattttt tgctacttct tcaggaatgc gaacgacaac
      241 ttctattcgc tcatcgcatt caggaggatt tccaatatga tgtcgttgaa gagtaacgcg
      301 ggcataatag tctttgattg ttagggaatc ttcgctcatg accggcatca tgggcagttt
      361 gcttgtccga agcaatacgg agcaggctgg acatccaccg gggtttgggg tcgaaatgag
      421 acccatgtct atcactccgc aagacctcca aaaactgctc gacgaacggg aggcacacag
      481 agcgccatgg aagcgtgccc gggagaatct tcaggaaatc cggtgggtcc tgaaagacgt
      541 agccgatgtg gatctgccgc caccggccag gaagacaatc gatctggaag gcagactggt
      601 taaggacggc gtgcggacag ctctcagcga ccgacaggat gctctgaggg atttggtcga
      661 ggctatccgc aagtaccgga agcttgccga gaaaacccct ctgacgttgc aggggcggat
      721 tttgcgcaag ctgtcgagga actgaacaag gctattgacc gggcagaagc cctccggtat
      781 gcgatagcct tactccccac acgcaaggcc tctatcttct cggggactgt gtttcaggat
      841 tcatcgcttc ccttttttct gatcgttttc ttttctctgt gcgcaacgag ccatgattgg
      901 ccgcgcaatc cacgcgactc taaaaatgtg tctagggctc tctctatttc attgaagccc
      961 cgagatggaa tctccaagcc gtcaatttcc gtcgccgcga atgtcctcct gaatctatgt
     1021 ttatcggtcg gtgtcattca ggctaaatcc ggcgcgtatc tgattaataa acagcatttg
     1081 aaagagagat gtgtcctttc aaggtcgccc tcattcacga gcgggagggt gatactcccg
     1141 tatgatttcg taattcttgc agagcgtaac tgactatcct tcaagaggct cccacgcttc
     1201 aagactcacg gcct
//
LOCUS       DATMKH010000501         1195 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_874840,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000501 DATMKH010000000
VERSION     DATMKH010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_874840"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /locus_tag="VNV63_05960"
     CDS             complement(<1..752)
                     /locus_tag="VNV63_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXC62200.1"
                     /translation="MAVSTSLNSGGFQSHGSGHRLFPSGQLIPFVLVTALFFLWGIPN
                     NLNDVLIRQFMKSFSISRLQAGLVQSAFYMGYFLLAMPAALVMRKVGYKAGFIIGLLL
                     YTLGAFLFWPAAFMGQYGYFLCALFVIASGLSFLETASNPFIAQLGDPDTSEQRLNFS
                     QAFNPFGAISGALIGTTFIFSGVELTPAEVAAFRSAGTYDAYLRTETLRVIEPYLVLG
                     CVTLFWAFLIWRTKFPAIHSEHENSGEDHGHFR"
     gene            complement(798..>1195)
                     /locus_tag="VNV63_05965"
     CDS             complement(798..>1195)
                     /locus_tag="VNV63_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014341638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXC62201.1"
                     /translation="LKQSKGSIVNIGSKTAVTGQGNTSGYASSKGAIMALTREWAVEL
                     LPYSIRVNTVIPAEVMTPLYRHWLDTFPNPEEKLDRIVSKIPLENRMTTSAEIANMTV
                     FLLSAKSNHTTGQHLFVDGGYVHLDRALT"
BASE COUNT          308 a          300 c          341 g          246 t
ORIGIN      
        1 cggaagtgtc cgtggtcttc tccgctgttc tcgtgctcgc tgtgaatggc gggaaacttc
       61 gtccgccaga tcaggaatgc ccagaaaaga gtgacgcagc cgaggaccag gtatggttcg
      121 attactctca gtgtttcggt ccgcaggtaa gcatcataag taccggcact ccgaaaagct
      181 gcgacctctg ccggcgtaag ttccacgccg gagaaaatga atgtggtgcc gattagagca
      241 cccgagatag cgccaaaagg attgaatgcc tgggaaaaat tgaggcgctg ttccgaagtg
      301 tcgggatcgc caagttgggc gatgaacggg ttcgatgccg tctcgagaaa cgacaaacca
      361 ctggcaatga caaagagtgc gcaaagaaag tatccgtatt gccccataaa ggcagccggc
      421 cagaacagaa aggcccccaa cgtgtaaagc agcagaccaa taatgaatcc agctttgtat
      481 ccgacctttc tcattaccaa cgcggccggc atagccagga gaaaatatcc catgtaaaag
      541 gccgactgca ccagtcccgc ctgcaaacgg gagatagaga atgacttcat gaactgccga
      601 atcaggacgt cattcaggtt gtttgggatt ccccacaaga agaagagcgc ggtcaccaga
      661 acgaaaggaa taagctgacc ggaagggaag agacggtgtc cggagccgtg cgactggaaa
      721 ccgcctgagt tcaacgaagt tgaaacggcc atggataatc cttgatcggc agataaacgt
      781 ttgaagcgcc tcaggactta cgtcaatgca cggtcgaggt gaacgtatcc cccatccacg
      841 aagaggtgtt gtccggtcgt atggtttgat tttgcggaaa gcaaaaagac cgtcatgtta
      901 gcgatctctg cggaggtcgt cattctgttt tccaaaggaa tcttgctgac gatcctatcc
      961 agcttctcct cgggattggg gaaagtgtcg agccagtgac ggtaaagggg agtcatgacc
     1021 tccgctggaa tcacggtgtt cacgcggatg ctgtagggca gcagctccac cgcccactca
     1081 cgggtgaggg ccataatggc tcctttggag gaggcgtacc cggaggtatt tccctggccc
     1141 gtgactgccg tcttcgagcc gatgttcacg atcgatccct tggattgttt caaat
//
LOCUS       DATMKH010000502         2202 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2470647, whole genome shotgun sequence.
ACCESSION   DATMKH010000502 DATMKH010000000
VERSION     DATMKH010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2470647"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1168
                     /locus_tag="VNV63_05970"
     CDS             <1..1168
                     /locus_tag="VNV63_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HXC62202.1"
                     /translation="ELLEAISAQIALVVENTQLFEMHEAKIQKLRTLEEISRALNSTL
                     VEKVVRHLAVEAAAKLMDAEMGCLLMIDPARKELFFEVALGERGEQVKEIRLKMGEGI
                     AGWVAQTGKPLIVDDVQKDPRFSQKADQKTDFVMRNMICVPVKIKGRTIGVLQAVNKK
                     NQGRFSKGDLEDFSSLANQVAIAIENANLYKELKDTFLSTAGALSDTIEAKDTYTGGH
                     TQRVMEYSLAIGWSLGLSPEEMENLKLAAILHDIGKIGVDDQVLRKQGRLNEEEKRKM
                     QQHTIIGPRIVKRVRQLHSIIPGIRHHHEMYDGSGYPDGVKGDEIPLMARIIAVADTF
                     DAMTSDRPYRKGLDMESALREIQKFSGKQFDPKMVQALQSVYEAGELERPVQIK"
     gene            complement(1175..1840)
                     /locus_tag="VNV63_05975"
     CDS             complement(1175..1840)
                     /locus_tag="VNV63_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXC62203.1"
                     /translation="MITVSDLHKNFETPQLLHILQGINLEIAKGELLCIVGASGVGKS
                     TFLHLLGGLDRPTLGKILYEGQNIAQLNENQLAEFRNKKIGFVFQFHHLLPEFTALEN
                     AMMPALIRGTPYQNAAERAKGILREVGLSQRFDHKPGELSGGEQQRVAVARALCLEPQ
                     VVLADEPTGNLDTHTGDEIFALLKDLNKRKGLTLVIVTHNEKLSAQADRIVRMVDGRI
                     EAV"
     gene            complement(1837..>2202)
                     /locus_tag="VNV63_05980"
     CDS             complement(1837..>2202)
                     /locus_tag="VNV63_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HXC62204.1"
                     /translation="SFNIVSTLTMIVVEKNREIAILKAMGATRHGVMRIFMLDGLIIG
                     LVGTAIGIPLGYAACEILQQFYTLPSDIYYISHLPVKIKGLDVLLVSLSAVIISFLAT
                     LYPSWQAAKLNPSEALRYE"
BASE COUNT          539 a          542 c          647 g          474 t
ORIGIN      
        1 ggaacttttg gaagcgatct ccgctcagat cgcgctcgtg gttgagaaca cgcaactctt
       61 tgagatgcac gaggccaaga tccagaagtt gcgcactctc gaggagatca gccgggccct
      121 caattcaacg ctggttgaga aagtcgtcag acatctggct gtagaggcgg ctgccaagct
      181 gatggatgcc gagatgggat gcttgctcat gattgacccg gcacgcaaag agcttttctt
      241 cgaggtggcc ttgggcgagc ggggagagca ggtaaaggag atccgtctca agatgggtga
      301 agggatcgcg ggctgggtag ctcagaccgg gaagccattg attgtagacg atgtccagaa
      361 agatccgcgt ttttctcaaa aggcggatca aaagactgat tttgtgatga ggaacatgat
      421 ttgtgtccct gtgaaaatca aggggcggac aatcggtgtg ttgcaagcgg tcaataagaa
      481 aaatcaagga agattttcaa agggagacct ggaggatttc agcagcctgg ccaaccaagt
      541 ggcgattgcg attgagaacg caaaccttta caaagaactc aaagacacat tcttgagcac
      601 ggcaggtgca ttgagcgata caatcgaggc aaaggacacc tataccggag gacatacgca
      661 gcgagtgatg gagtatagcc ttgcaatcgg ctggagtctt gggttgtctc cggaagagat
      721 ggaaaatttg aaactggcgg cgatccttca tgacatcgga aagatcggcg tcgatgatca
      781 ggttttgcga aagcaggggc ggttgaatga agaagaaaag cggaagatgc agcagcacac
      841 catcatcggt ccgcgcatcg tgaagcgggt ccggcaactc cattcgatca tccccggtat
      901 ccggcatcat catgagatgt atgacggcag cggttatccg gatggggtca aaggagatga
      961 gataccgttg atggcccgga tcattgcggt ggcggatacc ttcgatgcga tgacctcgga
     1021 ccggccgtat cgcaaagggc ttgacatgga gtccgcgtta agagaaattc agaagttttc
     1081 cggaaaacag tttgatccca agatggtcca ggctctccaa tccgtctatg aggccggaga
     1141 actagaaagg cccgttcaga tcaagtaggg agtgtcaaac agcctcaatt ctcccgtcca
     1201 ccatccgcac gatccggtcc gcctgagccg agagtttctc gttatgggtc acaattacga
     1261 gggtaaggcc ctttcgctta ttaaggtctt tcaaaagcgc aaaaatttca tcgccggtgt
     1321 gcgtgtccag gttgccggtc ggctcgtccg cgagaaccac ctggggctcc aaacagagcg
     1381 cgcgcgccac cgccacacgc tgctgctcgc cgcccgaaag ctctccaggc ttgtgatcga
     1441 aacgctggga taggcccacc tctcgaagaa ttccctttgc acgttcagcc gcgttttgat
     1501 aaggcgtgcc gcggattaat gccggcatca ttgcgttctc cagcgccgtg aattccggga
     1561 ggaggtgatg gaactggaac acgaaaccga ttttcttgtt ccgaaactca gccagttggt
     1621 tctcattcaa ctgagctatg ttctgtcctt cgtataggat cttaccgagc gtcgggcggt
     1681 ccagcccgcc caagagatga agaaaggtgc tcttccccac acccgaggcg ccgacgatgc
     1741 acaggagttc gcctttggcg atctcaagat tgatgccctg cagtatgtga agcagctgcg
     1801 gcgtttcaaa atttttgtgg aggtcgctga ccgtgatcat tcgtaacgca gggcctctga
     1861 aggattcagt ttggccgcct gccaggaggg gtagagtgtt gcaaggaagc tgatgatgac
     1921 cgccgaaagg gagactaata gaacatcgag ccccttgatc ttcacaggga ggtggctgat
     1981 gtagtagata tcgctcggca gcgtgtaaaa ctgctggagg atttcacaag ccgcatagcc
     2041 caacgggata ccgatggcgg tcccaacgag gccgatgatt aggccgtcca gcatgaaaat
     2101 ccgcatcacg ccgtgccgtg ttgctcccat cgccttgaga atcgcgattt cccggttctt
     2161 ctccacgacg atcatcgtga gcgtgctgac gatgttgaac ga
//
LOCUS       DATMKH010000503         1007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1518221, whole genome shotgun sequence.
ACCESSION   DATMKH010000503 DATMKH010000000
VERSION     DATMKH010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1518221"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="VNV63_05985"
     CDS             <1..835
                     /locus_tag="VNV63_05985"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXC62205.1"
                     /translation="VTHTVLVPSMVNSLCQFEELHEYNLESLEVLAYGGSPIAPALIK
                     DIRRLLPKTRLLQVYGLSEAGFLTGLTDGEHTNDRLQSCGRTCPGIDLRVVHTNGRPA
                     VAGEPGNLIARGPSIMVGYWQDIDEDLPKDESAVDETTKAFCNGFFQTGDIGFQDKDG
                     YFFIVDRAKDMIVSGGENVYSGEVEAAIYEIPGVREAAVFGIPDEKWGEVVAAAVVLR
                     SGTNLSAEELKRYCKTRIASYKVPRHIEFMTEELPKSGSGKILKRVLREKYWAGQSRC
                     V"
BASE COUNT          254 a          245 c          294 g          214 t
ORIGIN      
        1 ggtgacgcac accgttctcg tcccctccat ggtcaattct ctttgccaat ttgaggaact
       61 tcatgagtac aacctggaat ctctggaagt gctggcctat ggaggctcac caatcgctcc
      121 ggcgctcata aaggacatac gcaggctcct gccgaagaca aggctgctgc aagtctacgg
      181 gttgagcgag gccggtttcc tgacggggtt aacagatggg gaacatacca atgacagact
      241 tcagtcctgt ggacgcactt gccctggcat cgatctgaga gttgtccata caaatggaag
      301 acctgcggtt gccggcgaac caggcaacct gatagcgcgg ggcccgagca tcatggtggg
      361 ctactggcag gacatcgacg aagacctgcc gaaggatgag tctgcagtcg acgaaaccac
      421 aaaagcattc tgtaacggtt tctttcaaac cggagacatc ggctttcaag acaaagacgg
      481 gtacttcttc attgtcgatc gagcaaaaga catgatcgtc agcggcggtg agaatgtcta
      541 ctccggtgaa gttgaggccg ccatttatga gatccccggt gtgagagagg ccgcagtgtt
      601 cggcatcccg gacgagaaat ggggagaggt ggtggccgca gccgttgttc tacgatcggg
      661 aaccaatttg tccgcagaag aactcaagcg gtactgcaaa acgcggatcg cctcatacaa
      721 ggtaccccgc catatcgagt tcatgacgga ggaactcccc aagtccgggt cgggcaagat
      781 tttgaagcga gttttacgtg agaagtactg ggcagggcaa tccaggtgtg tgtgatgctc
      841 tgcccgccgg aagaaggttt tgaagcagga aggggatcca gaacatcgtg acgagcttcg
      901 ctcttcaaga tgacaaacaa atggagatgt tggagatctt atgtgcgaaa acaaaatctt
      961 ttcaggaatt aaggtagttg accttgcgag cttcatcgcg ggtcccg
//
LOCUS       DATMKH010000504         3687 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_236071,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000504 DATMKH010000000
VERSION     DATMKH010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3687
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236071"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="VNV63_05990"
     CDS             complement(<1..524)
                     /locus_tag="VNV63_05990"
                     /inference="COORDINATES: protein motif:HMM:NF015699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HXC62206.1"
                     /translation="MTGYGRSEGHHQNHTMVVELRSVNHRYFEVMLRLPKPLLSIETE
                     FKKLIQDRFPRGRVDCSITLNGEGESVKQLSLNRELAKQYCSLLETLKQELGLEGNVD
                     LALLANIPGLIVVTEPPMLLEELGTAANRLLKKAMDSLDEMRQREGKQLYQDLMDRIK
                     VLRQALKKVSSRAP"
     gene            699..1130
                     /locus_tag="VNV63_05995"
     CDS             699..1130
                     /locus_tag="VNV63_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62207.1"
                     /translation="MSNLTSNQQHLKNFQNLVSQLIEYNQHIPVVVEGQRDVESLKRM
                     GLTGEILKLHSGKSLYDFCEELAKQYPQFILLMDWDERGNDLLAKITRHLETGWQEFS
                     HFRSGFITLCGRHFQEVENLASWFETLQAEAAPQGSSCAGL"
     gene            1275..1535
                     /locus_tag="VNV63_06000"
     CDS             1275..1535
                     /locus_tag="VNV63_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 domain-containing protein"
                     /protein_id="HXC62208.1"
                     /translation="MGRGLLSAFVATFPAITLVTMIFIYREGGYTAAYSYAKGLALFF
                     PAWFAYLMFVVFTLPRWGIWKALAGALLLFMTLVALIRLAVR"
     gene            complement(1539..2330)
                     /locus_tag="VNV63_06005"
     CDS             complement(1539..2330)
                     /locus_tag="VNV63_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HXC62209.1"
                     /translation="MRIGFFQFAPVFGEVKTNVEYAIRRLSGLEADLMVLPELFTSGY
                     QFVSKQEVRELAEEIPKGPTTSRLIELARDRKMIIAAGLPERKGKRYFNSSVLVGPKG
                     FITVYRKIHLFYEEKLWFSPGDIEFRVHHIGGVRIGIMVCFDWFFPESVRSLALSGAE
                     IICHPSNLVLPYCPDAMVIRCLENRVFAVTANRIGAEQRGGKPPLTYIGQSEVVNPKG
                     MILYRAKPDEEDIAVVEVNPKEARNKRINTYNDLFRDRRTKFYKR"
     gene            complement(2348..>3687)
                     /locus_tag="VNV63_06010"
     CDS             complement(2348..>3687)
                     /locus_tag="VNV63_06010"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012964096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HXC62210.1"
                     /translation="LLADLFSKAQASEAKIISRFVVGRLRLGVGDAAVLEALSLARLG
                     SREHRAELEQAYNFCSDLGLVAKTLFENGLEGINKIKVRPGYPIRMELCERLSSAEEI
                     IAKIGRCALEAKYDGFRCQIHKVNDRIEIFSRSLERTTPMFPEIVQAVKTHLKAETLI
                     FEGEALAYQEATGELLPFQVTMQRKRKHGIEDIAKEFPLKFYAFDLLYLDGRDCTDQP
                     YTERRKKLGQLIKKDAVIELSEGIETGDPKEVQRFFDDMIERGLEGIVVKRLDAPYSA
                     GARNFNWIKLKRSYKGELSDTVDVCIVGYFKGKGHRAQFGIGTVLGAVYDEETDSFKT
                     ISKIGSGFTEEELTKLSEMLDKIAVPHKPARVESLMTPDVWVEPRYVVTVTADEITRS
                     PLHTAGKKGDEPGYALRFPRVEGFIREDKGPQDTTTVKEVIKLAQQQKRVKLE"
BASE COUNT          839 a         1052 c          884 g          912 t
ORIGIN      
        1 tggggcgcac gagaactcac cttttttaaa gcctgccgca gaaccttgat gcgatccatc
       61 aagtcctgat aaagctgctt gccctcgcgc tgacgcatct catctaaaga gtccatggcc
      121 ttcttgagca gcctgttagc cgccgtaccg agttcttcca gcagcatggg gggctccgtt
      181 acaacgatca agcccggtat gttggccagc agcgccagat ccacatttcc ttccagaccg
      241 agctcctgtt tcaacgtctc caataaagag cagtactgct tggccaattc ccgattaaga
      301 ctgagttgct tgaccgactc cccttcgcca tttaaggtga ttgagcagtc aacgcgccct
      361 cggggaaaac gatcctggat taatttcttg aactcagttt cgatggaaag cagaggcttg
      421 ggcagcctca gcatcacctc aaaatagcgg tggttcaccg atcggagctc caccaccatc
      481 gtgtgatttt gatgatgtcc ctcgcttcga ccatatccgg tcatacttcg tatcatctta
      541 aaaattcatc actataaatt gtgaattata tggataatct tctcgattgt caatctttgt
      601 cgctgctcta aaattttgac aaggccgttc tgtttcggta cactagatac tgtccaagga
      661 agaactttat tatacaacct aagaatgagt aaaagccagt gagtaattta acatccaatc
      721 agcagcatct caaaaatttt cagaatctgg taagccagtt aatcgagtac aatcagcata
      781 ttcctgtggt cgtggaagga cagcgggatg tggagagtct caagcgcatg ggtctgaccg
      841 gtgagattct gaaactgcac agcgggaagt ctttgtacga cttctgcgag gaactggcca
      901 aacagtatcc tcagttcatt ctgcttatgg actgggacga acgcgggaac gatctgctgg
      961 caaaaatcac acgccacctg gaaacaggct ggcaggagtt cagccatttc cgatccggct
     1021 tcatcacgct ctgtggcagg cattttcaag aggtggagaa tcttgcctcc tggtttgaaa
     1081 ccctgcaagc ggaggcagca ccccagggat caagctgcgc gggactttga ctgcaactca
     1141 ttactaagga taaaaaatag tcgctttacc gcagtggttc tttttcttag aaacgaaagg
     1201 cgccgacaat caagttcatt ctgtatttct tgctgggcgg gacaatcatc agccttgttt
     1261 cttatctggg tagcatgggg cgagggctcc tctcggcctt tgtggccaca ttcccggcga
     1321 tcacgctggt cacaatgatc tttatctatc gggaaggagg gtacacggct gcctattcct
     1381 acgccaaggg ccttgccctc ttttttcccg cgtggtttgc gtatctcatg tttgtcgtct
     1441 tcacactgcc ccggtggggc atctggaagg ccctggccgg tgcacttctc ctgtttatga
     1501 cccttgtggc cctcattcgc ctggcagtcc gttaacactt aacgcttata gaatttcgtt
     1561 cttcgatcgc gaaaaaggtc attgtaggtg ttaattcgtt tgttgcgggc ctctttggga
     1621 ttaacctcga ccacagcgat atcttcctcg tccggcttgg cccgatacag aatcatacct
     1681 ttcgggttta ccacctcgct ctgtccgatg taagtgagcg gcggtttccc tccacgctgc
     1741 tcggctccga tccggtttgc ggtcaccgca aagacacgat tctccagaca tcggatcacc
     1801 atcgcgtccg ggcagtacgg gaggaccaga ttagaaggat ggcagatgat ctcggccccg
     1861 gaaagcgcca aactccggac cgattccgga aagaaccagt caaagcagac cattattccg
     1921 atcctgacac cgccgatatg atgaactcgg aattcgatgt ctccgggaga aaaccagagc
     1981 ttctcttcat aaaagagatg aatcttccgg taaacggtga taaaaccttt ggggccgacc
     2041 agcacagagg agttgaagta tcgcttgcct ttgcgctccg gcagacctgc cgcaatgatc
     2101 atctttcgat cccgggctag ttcgatcagg cggctggtag tcgggccctt agggatctct
     2161 tccgccaatt cccgcacttc ctgttttgaa acaaactggt acccgctggt aaacaactcc
     2221 ggcagtacca tcaagtcggc ttcaagtccg gacaaccgac ggatggcata ctccacgttt
     2281 gttttaactt caccaaaaac tggtgcgaat tgaaaaaacc cgatcctcat ttttatcccg
     2341 accgtcccta ctccagcttc acgcgctttt gctgctgggc aagcttgatc acttctttga
     2401 ccgtcgtagt atcctgagga cctttgtcct ctcggataaa accctcgacc cgtggaaacc
     2461 ggagcgcata accaggctcg tctccctttt ttcccgccgt gtggagcggt gaacgggtga
     2521 tctcgtccgc ggtcacggtc accacatatc ggggttccac ccagacatcc ggggtcatga
     2581 gggactcgac acgggccggt ttatgaggaa ccgcaatctt gtccagcatt tcggacaact
     2641 tggtcaactc ctcttccgta aaaccggaac cgatcttgct gatcgtctta aaggagtcag
     2701 tctcttcatc gtacaccgcc ccgagcaccg tgccgatgcc aaactgtgct ctatgccctt
     2761 ttcccttgaa gtaccccaca atacagacat ccaccgtatc cgatagctcg cccttgtagc
     2821 tgcgtttgag cttgatccag ttaaagttcc tggcaccggc cgagtaaggg gcgtccaacc
     2881 gcttgaccac gatcccctcc aacccccgct cgatcatgtc gtcaaaaaat cgctggacct
     2941 ccttgggatc acccgtctcg atcccttcgg acaactcgat aacagcatcc tttttaatca
     3001 actgccccag ttttttccgc cgctcggtat aaggctggtc cgtacaatcc cgtccatcga
     3061 gatacaaaag gtcgaaggca taaaacttga gcggaaattc ctttgcgatg tcttcaatgc
     3121 cgtgcttgcg ttttcgctgc atcgtcacct gaaacggcag caattcaccg gttgcctcct
     3181 gataggccag cgcctccccc tcaaagatca gggtctcagc cttgagatgg gtcttgaccg
     3241 cctggacgat ctccggaaac atcggtgtgg ttcgttcgag gcttcgagag aaaatctcga
     3301 tccggtcatt gaccttgtgg atctggcagc ggaagccgtc gtacttagcc tccagggcgc
     3361 accgaccgat cttggcgata atctcttcgg ccgatgagag ccgctcgcaa agctccatcc
     3421 gaatcggata gcccggccga accttgatct tgtttattcc ttcaagtcca ttttcaaaaa
     3481 gcgtcttggc caccaaccca aggtctgaac agaaattata ggcctgttcg agctcggccc
     3541 gatgctcccg gctgcccagc cgggccaggc tcagcgcctc cagcaccgcc gcatccccaa
     3601 cccccagacg gagtcggccc acaacaaaac gagaaatgat ttttgcctcc gaagcctgtg
     3661 ccttggaaaa gaggtctgcg agaaggg
//
LOCUS       DATMKH010000505         1137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4406153, whole genome shotgun sequence.
ACCESSION   DATMKH010000505 DATMKH010000000
VERSION     DATMKH010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4406153"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="VNV63_06015"
     CDS             <1..472
                     /locus_tag="VNV63_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62211.1"
                     /translation="EIVPCRLAQPWWLVIVNPGFQVSTAWAYQQLAAERKESKTGLTN
                     SPERTRMSSLNSVPDIRRTGGRKGRRIVVKEVLGRLDNSLESVTGAHYPVILDIKAQL
                     LAAGAQGALMSGSGPTVFGLFSNRSIAKAAADRFKSHPGWRIWIVKTLQRLPKR"
     gene            507..581
                     /locus_tag="VNV63_06020"
     tRNA            507..581
                     /locus_tag="VNV63_06020"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:540..542,aa:Gln,seq:ttg)
     gene            608..>1137
                     /gene="prs"
                     /locus_tag="VNV63_06025"
     CDS             608..>1137
                     /gene="prs"
                     /locus_tag="VNV63_06025"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HXC62212.1"
                     /translation="MHNEIKLFSGNSNLPLAQEIADYLGISLGEAMVSNFSDGEILVR
                     IEENVRGADVFVLQSCADPVNKNILELFIMVDALKRSSASRITAVIPYYGYARQDRKD
                     QPRVPITAKLVADLLTASGTHRVLTMDLHTGQIQGFFNIPVDHLYAMPVLLEDIRKRN
                     IADLVIVSPDAGGVERA"
BASE COUNT          240 a          291 c          345 g          261 t
ORIGIN      
        1 agagattgtt ccgtgtcgct tagcgcagcc ctggtggctt gtgattgtga atccaggctt
       61 tcaggtctcg accgcctggg cctatcagca gttggcagca gagaggaagg aatccaaaac
      121 agggttgaca aactccccgg agaggactag aatgtcgtcg cttaattccg tgcctgatat
      181 ccgtcggacc ggcgggcgga aggggcggcg aatcgttgtg aaagaggtct tgggccgtct
      241 cgataacagt ctcgagagcg tcacaggcgc ccattatcca gtcatcttgg atataaaagc
      301 acagttatta gccgcagggg ctcagggcgc gctgatgtcc gggagcggac ccacggtctt
      361 cggtcttttc tccaatcgga gcattgcaaa agcggcggca gaccggttca agagccatcc
      421 gggctggcgg atttggatcg ttaagaccct ccagcgattg ccaaagaggt gaaggcatcg
      481 taaggccttt gcagcagata ttctgttcgg aggtcgtcca atcggtagga catcagcctt
      541 tggagctgag aatcggggtt cgagcccctg cctccgagcc aagtgtagcg atacggagtt
      601 cttgagaatg cacaatgaga taaagctgtt ttcaggcaat tcgaatcttc ccctggccca
      661 agagatcgcc gattatcttg gtatttctct tggggaggcg atggtctcca actttagtga
      721 tggagagata cttgtccgca ttgaggagaa tgtccgcggc gcggatgtct tcgtgcttca
      781 gtcctgcgcc gatccggtca ataaaaatat cctggagctc ttcattatgg tggatgcctt
      841 gaagcgctcc tcggccagcc ggatcacggc ggtcatcccg tattatgggt acgcccgtca
      901 agaccggaag gaccagccgc gggtcccgat cacggccaag ttggtagctg atttgctgac
      961 agcatccggg acccatcggg tcctgacgat ggatctccat acggggcaga tccagggttt
     1021 tttcaacatt ccggtagacc atctctatgc gatgccggtg ttgcttgaag acatccggaa
     1081 gcggaatatc gccgatctgg tcatcgtttc ccccgatgcg ggcggcgtgg agcgggc
//
LOCUS       DATMKH010000506         3944 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1200916, whole genome shotgun sequence.
ACCESSION   DATMKH010000506 DATMKH010000000
VERSION     DATMKH010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3944
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1200916"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            242..1579
                     /locus_tag="VNV63_06030"
     CDS             242..1579
                     /locus_tag="VNV63_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HXC62213.1"
                     /translation="MTSSITLAQDQTAPPPAAAPAAPPAAAPAAAPAAPLKVDSGDTA
                     WLLTSSALVLLMTAPGLALFYCGMVRRKNVLGTMMHSFVILCLISVQWVLYGYSLSFG
                     PDHGHIIGGLEWFGLNGVGLEPNGDYGATVPHQAFMVFQLMFAAITPALITGAFAERV
                     KFSGFLLFTLLWATFIYDPLAHWVWGMGGWLHNLGALDFAGGTVVHISSGVSGLAAAL
                     YLGKRVGYGKEPMAPHNLPFTVIGASLLWFGWFGFNAGSAIASNGLATSAFVVTNTAT
                     AAAALAWMFTEWAVRGKPTVLGVASGEVAGLVAITPASGFVGPMPSIIIGVGAGILCY
                     FATSKIKNALGYDDTLDAFGVHGIGGTWGAIATGLFASKAINSGGADGLFFGNPGQVW
                     LQIVAVGASWVLAFVGTFILLKVVDILVGLRATPEEERIGLDLSQHNESGYTL"
     gene            1609..1947
                     /locus_tag="VNV63_06035"
     CDS             1609..1947
                     /locus_tag="VNV63_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HXC62214.1"
                     /translation="MKKIEAIIKPFKLDEVKAALNDIGVQGMTISEVKGYGRQKGHKE
                     TYRGTEYTIEFIPKVKIEVAVSDQQAQKVIEAIMRTAKTGSIGDGKIFVIDLKESIRI
                     RTGETGESAL"
     gene            2014..>3944
                     /gene="glnD"
                     /locus_tag="VNV63_06040"
     CDS             2014..>3944
                     /gene="glnD"
                     /locus_tag="VNV63_06040"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HXC62215.1"
                     /translation="MVTEVQKFLERRRSEILKAHANRASGKEVVSALTAVADEAIGKL
                     YLDAMVRSRATCPSWSGEDLVLVALGGYGRQELAPYSDIDIMFLYREDGSETARDVAA
                     TILRSLWDLGYRIGHSQRTIQDCVTIGKADLTVKTSLMEARFLIGGRSLYREFHKRYQ
                     QEVVHKKVSAYITDKIEERSKEYERYGSTLYLLEPDIKMSKGGLRDLHLLKWVAMARY
                     QTFSLSDIYQRALLSPKDFQILMETQEFLWKIRNELHFHAGRCYDVLSFDEQVRLAEV
                     FQFEDQHKRLAVESFMQRYYQHTTGLHHVATRFLNRAQPRSVWQRLGDRFLSNIVEQD
                     FLVMRDTISVLPAARPEILADPVRLLRLFYLSQHHGVRISDELLEMIHEQMEAMPAEL
                     FQSKEVGKLFLAILGGTGQTDRQAGRVAETLAAMHQVQLLGKILPVFSSVRGLMQFNE
                     YHKYTVDEHCIRSVQEAETMIGQKGYLEGVYREIHRKDILHLALLLHDLGKGEPGDHS
                     LIGKRIAMETADRLGLNEHERGLLGLLVARHLLMSDLAFRRDLTDQKVLLLFARQVAT
                     PEALKMLLILTAADIAAVGPGVRTPWKEDLLLELYLKGMEELTGTKPLVSEEKRLADI
                     RTAVTKQLAGRYPESWLDE"
BASE COUNT          860 a          944 c         1158 g          982 t
ORIGIN      
        1 tttacgaagg atatttctct cttgaccggt ccggtttggt ttgaagatag gacaatcaac
       61 ggcgcttgga aatggacgac tcagctcgat atcaattttt agtatgaccg gaatcacttg
      121 gtgcaaaagc cacaacctat aaaagatgga ggttagcagt aaaaggagga gttaaagaat
      181 gaagcattgg aagaaaattt gctgcggcat cgtcacatta ctgtttgtaa gtttagtgtt
      241 tatgacttca agtatcactt tagctcagga tcagaccgct ccaccaccgg ctgccgcacc
      301 ggccgctcct ccagccgcag caccagccgc agcgccggcc gcaccgctta aggtagattc
      361 gggagatacc gcgtggctcc tgacctcttc tgccctggtg cttttaatga ccgcgcccgg
      421 cttggcgctg ttctactgcg gaatggtccg tcgaaagaat gtcctgggta cgatgatgca
      481 cagttttgtg atcctctgtt tgatcagcgt ccaatgggtc ctctacgggt attccttgtc
      541 ttttggaccg gatcacggtc atattatcgg agggctggaa tggtttggat tgaacggggt
      601 tgggttggag cctaacgggg attacggcgc aactgtaccg caccaggcct tcatggtctt
      661 tcagttaatg ttcgctgcga ttacaccggc cctgattacc ggcgcctttg ccgaacgggt
      721 caagttcagc gggtttttgc ttttcacatt gctgtgggct acttttatct atgacccgct
      781 ggctcactgg gtttggggaa tgggtggctg gctgcacaat cttggcgcgc ttgattttgc
      841 gggcggaacc gtggtgcata tcagctcggg cgtctcaggt ctggctgccg cactctatct
      901 tggtaagcgc gtgggatatg ggaaagaacc catggcgcct cacaacctcc cgttcacggt
      961 gatcggcgct tcccttcttt ggtttggctg gtttgggttc aatgccggaa gcgcaatcgc
     1021 gtccaacggt cttgccacaa gtgcttttgt cgtgacgaat accgccactg ccgctgcggc
     1081 cctcgcctgg atgtttactg aatgggctgt tcggggcaaa ccgacagtat tgggtgtagc
     1141 gagcggtgag gttgccggcc tggttgccat caccccggct tctggatttg taggaccgat
     1201 gccttccata attattggag tcggtgccgg tattctctgc tattttgcca cgagcaagat
     1261 aaagaacgcg ctgggctatg atgacacgct cgatgccttc ggcgtccatg ggatcggagg
     1321 gacctggggc gcaattgcta ccggtctttt cgcatcgaag gccattaact cgggtggtgc
     1381 cgatggtctg ttctttggca atcccggtca ggtgtggttg cagatcgttg cggttggcgc
     1441 ctcatgggtt ctcgcctttg tcgggacctt tattcttcta aaggttgttg atattcttgt
     1501 gggcctcagg gccactccgg aagaagagcg cattgggctc gatttaagcc agcacaatga
     1561 aagcggttat accttgtaaa ccaacgatag attaataggg agatagacat gaaaaaaatc
     1621 gaggcgatca tcaaaccttt taagttggat gaggtcaagg ctgccttaaa cgacataggg
     1681 gtgcaaggga tgaccatcag cgaagtgaag ggttatggac ggcagaaagg acacaaggaa
     1741 acctatcgtg gaacggagta cacgattgag tttatcccca aagtgaagat tgaggtggcg
     1801 gtctcggatc agcaggctca aaaggttatc gaggcgatca tgcgaaccgc caagacgggg
     1861 agtatcgggg acggcaaaat ttttgtaatc gatcttaagg aaagcatccg gatcagaacc
     1921 ggagagacag gcgaaagcgc cctttagatt tggcgatggt gggggcgcat gcgtgcgccc
     1981 ccgcagggga ttgctctcaa cggggagagc gttatggtga cagaggtcca aaaattttta
     2041 gagcggcgac gatctgaaat cttaaaggcc catgccaacc gggcttctgg aaaagaggtt
     2101 gtctcggcct tgacagctgt cgcggatgaa gccattggaa aactttatct tgacgccatg
     2161 gtccgctccc gtgccacctg cccttcctgg agcggtgagg atctggtcct agtggcgctg
     2221 ggaggctatg ggcggcagga gctggcaccc tactcagata ttgatattat gtttctctac
     2281 cgggaagatg gctctgagac cgcccgggat gttgctgcga cgatcctccg gagcctctgg
     2341 gatctcggtt accgtatcgg ccacagtcag cgcacgatcc aggattgtgt gaccatcgga
     2401 aaagctgacc tgacggtcaa aacctctctc atggaggcta ggtttcttat cgggggtcgt
     2461 tccttgtatc gggaatttca caagcggtat cagcaggagg tcgttcacaa aaaggtgtct
     2521 gcctatatta ccgacaagat cgaagagcgg tccaaggaat atgagcgtta cggttcaacc
     2581 ctctatctct tggaaccgga tattaagatg agcaagggag ggttgagaga ccttcacctc
     2641 ttaaaatggg tcgcaatggc gcgttatcag accttttcgc tttcggatat ctaccaacgg
     2701 gcgctgcttt ccccgaagga tttccagatc ttgatggaga ctcaggagtt tctctggaaa
     2761 atcaggaacg aattgcattt tcacgcgggc cggtgctacg atgtcctctc ttttgacgag
     2821 caggttcgcc ttgccgaggt ttttcagttt gaggatcagc acaaacggct ggcggtcgag
     2881 agcttcatgc agcggtacta ccagcacacc acggggctac atcatgtcgc cacgcgtttc
     2941 ttaaaccgtg ctcagcctcg atccgtttgg cagcggttgg gtgatcggtt cttatcaaat
     3001 attgttgaac aggattttct agtgatgcgt gatacgattt cggttcttcc cgccgcgcgg
     3061 ccggagatct tggctgaccc ggtgcggctc ttacgccttt tttacctctc tcaacatcac
     3121 ggcgtccgaa tttcagatga actccttgag atgattcatg aacagatgga ggccatgccg
     3181 gcggagttgt tccagtcgaa agaggtagga aagttatttc tggcgattct tggcggaacc
     3241 ggccaaactg atcggcaggc aggccgcgtc gctgagaccc tggccgcaat gcatcaggtt
     3301 cagctgctcg gaaaaattct cccggtcttt tcttcagtgc gcggactgat gcagtttaat
     3361 gaatatcaca aatataccgt ggatgagcac tgtatccgtt cggttcaaga ggcagagacg
     3421 atgatcggcc agaagggcta cctggaaggc gtctatcgag agatccatcg caaggacatt
     3481 ctccatcttg cgctgctgct tcatgatctg ggcaagggtg agccggggga tcatagcttg
     3541 atcgggaaac ggatcgcgat ggaaacggct gatcgtctgg gtctgaatga acatgagcgg
     3601 ggtcttctcg gccttctcgt ggcacggcac ctgttgatga gtgatcttgc cttccggcgg
     3661 gatttgacgg atcaaaaggt tcttctgctt tttgcgagac aagtggcaac accggaggcg
     3721 ctaaagatgc ttttgatttt gaccgcggcc gatatcgcgg cggtcggtcc gggggtccgg
     3781 acgccttgga aagaagatct tctgcttgaa ctttacctaa agggaatgga ggaactcacc
     3841 gggacgaaac ctctggtgtc tgaagagaaa aggctggcgg acatacggac ggctgtcact
     3901 aagcaactcg cggggcgtta tccggagagc tggctggatg aaca
//
LOCUS       DATMKH010000507         1519 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2138600, whole genome shotgun sequence.
ACCESSION   DATMKH010000507 DATMKH010000000
VERSION     DATMKH010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2138600"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1519
                     /locus_tag="VNV63_06045"
     CDS             <1..>1519
                     /locus_tag="VNV63_06045"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HXC62216.1"
                     /translation="PNKPEHQTALNQVLKRLEARDHVAVSLRLMNNGKFSDALDELQK
                     AFAVVPGYPPAQEAMAKLSEKRRTSKNEDENLALKSSQPITLKFQNARLKDVFELLSK
                     TTGINILFDKDVRDDFVSIFIKDASFREVLNLILTTNNLFMKKISEDTILIVPRVANK
                     LSQYQDLMIQTFYLSNVKAKDMINLLRTMLETKRIFVNDELNAIVIRDTADKMKLAEK
                     IIDANDRNPSEVMFEIEVLEVDKTSATQIGWNLNPAQATLGLTPNPITFTQLKALGND
                     SYLFTLPSIIVDFMKAESDAKTLANPKIRVMDNKTAKVTIGNKFPVLISTSTFSPTGT
                     TAAGGVTAAGTSLSTSVEYKDVGIKIQAEPNIHLNNDVTLKLNLTVSSLGNLVNLGNN
                     TSQYEFGERNAETVLNIRDGETVIIGGMIQDEDRTSVTKVPGLGDIPVLGKLFSDTGK
                     SKVTTDIVLTITPHIVRSLETPGKEAQAFWSGTEEAYSTKPLFSEQPIADEIKESQ"
BASE COUNT          427 a          368 c          385 g          339 t
ORIGIN      
        1 cctaacaagc ctgaacacca gactgctctg aaccaggtat tgaaacggct ggaggccaga
       61 gaccatgtgg cggtcagtct tcgcctgatg aataacggta aattttcaga tgcactggat
      121 gagcttcaga aggcgtttgc ggtggttccc ggctatccgc ctgcccagga ggcgatggcc
      181 aagctgtctg aaaaacggcg aacgtcaaag aacgaagatg aaaatctagc cctcaaatcc
      241 agtcaaccga ttaccttaaa gttccaaaat gccagactca aggatgtgtt tgagcttctc
      301 tcgaagacca ccgggatcaa tatcctgttc gataaagatg tccgggatga ttttgtgagc
      361 atctttatca aggacgcttc ttttagagag gttttaaacc tgattctgac aaccaacaat
      421 ctctttatga agaagatcag cgaggatacg atcctgatcg tccccagggt agccaacaag
      481 ctgagtcaat accaagacct catgatccag accttttacc tttccaacgt caaggccaag
      541 gacatgatca acctcctgcg aactatgctg gagaccaaga ggatcttcgt gaatgacgaa
      601 ctcaatgcca ttgtcatccg ggacacggcg gataagatga agctggctga aaagatcatt
      661 gatgccaacg atcggaaccc gtccgaggtg atgtttgaga tagaggtctt ggaggtggac
      721 aagacgagtg ctacgcaaat cggctggaat ttaaatccgg cgcaggcgac tttggggttg
      781 actcctaatc ccattacgtt tactcaactc aaagcgcttg ggaacgactc ttatctcttc
      841 accttaccca gcattattgt agactttatg aaggccgaat cggatgccaa gacgctggcc
      901 aatccgaaaa tccgtgtcat ggataataaa acggctaagg taaccatcgg aaacaagttc
      961 ccggttctga tcagcaccag cactttttca cctacgggaa ctacggcagc aggaggggta
     1021 acggcggctg gaaccagtct ttccacaagc gtggaatata aagatgtcgg tattaagatt
     1081 caggcagagc ccaacattca cttgaacaat gatgttaccc tcaaactcaa tctcacggtg
     1141 tcttctttag gaaatctggt aaatttagga aacaacacgt cgcagtatga gtttggtgag
     1201 cggaatgcgg agacggtgct gaatattcgt gatggcgaga ccgtaatcat cgggggcatg
     1261 attcaagatg aagatcggac ttcggtcacc aaggtcccgg gtctcggcga catcccggtg
     1321 ctcggcaagc tgttctccga cacggggaaa agtaaggtca cgaccgatat cgtgctgacg
     1381 atcacgccgc atatcgtacg gtcactggaa acgccgggta aagaggctca ggccttctgg
     1441 tcagggacag aggaagccta ttccaccaag ccgctcttct ccgaacagcc catagcggat
     1501 gaaatcaaag agtcgcaga
//
LOCUS       DATMKH010000508         1915 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4706902, whole genome shotgun sequence.
ACCESSION   DATMKH010000508 DATMKH010000000
VERSION     DATMKH010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4706902"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="VNV63_06050"
     CDS             <1..341
                     /locus_tag="VNV63_06050"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HXC62217.1"
                     /translation="TPIVDEHGRLAGIITRSDAMRALGQDGNLTVLDTGSSDLIVAFP
                     DELLSHAVTKMLRNNIGHLPVVNRKDPLHLIGYLERSNILEARLRQLNDEHVREHGWF
                     HNLFLKIMAR"
     gene            346..1146
                     /locus_tag="VNV63_06055"
     CDS             346..1146
                     /locus_tag="VNV63_06055"
                     /inference="COORDINATES: protein motif:HMM:NF022447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallocarboxypeptidase"
                     /protein_id="HXC62218.1"
                     /translation="MRRKIRSYRQVVKRLEAVARNETGLQLKRLGKIKSNPADYDLYL
                     IRSQKAPRFFRRRICLCAGIHGDEPAGVEAILTLIEDRRRLREILDELHLTIFPCINP
                     YGFEHNTRTNGLGLDLNRQFRSRRPPVEVSLVKQAIGRTCYDLAMEFHEDVDTNGFYL
                     YELTAGKEPAWGPLIVEKVGLHWPVNRGEEIEGMKSKNGVIHRKNQNGSFRQLMQTRP
                     DWPQAFYFFSHGTRHCFTTETPVTLDIKDRAKIHLTALEVSLRQLTLS"
     gene            complement(1143..>1915)
                     /locus_tag="VNV63_06060"
     CDS             complement(1143..>1915)
                     /locus_tag="VNV63_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62219.1"
                     /translation="EPNVLYEYVHGFDSTDIPQLDQVDAFPQKNLLTYSLINRLVLKR
                     TGEGGTTKRLEIFYLKFTQSYGRERILNLPPQRSFSDFRGEMILRASRRLSLHVDSFL
                     DVYNPHRFTTVDTDLKFHLNEILLLSVGERYTRAGTTPQRGDLLEPFSLGLQQAQAAD
                     LKFLTMGAVVNIASRVSLAGKIFYDYDQGQLAEARAAVRYTGSCHCWGFTVSYIHSEA
                     TGINNIEPVKNDVSFFFSVTGISNTESSVVKKLFEPLD"
BASE COUNT          487 a          504 c          497 g          427 t
ORIGIN      
        1 gaacaccgat tgtagatgaa cacggacgat tggctggaat aattacgcgg agcgacgcga
       61 tgcgcgcgct tggtcaggac ggcaacttga cggtactgga taccggcagc agcgatttga
      121 tcgtggcctt tccagacgaa ctcctgagcc atgccgtcac caagatgttg agaaataata
      181 ttggacactt gcccgtcgtg aaccggaaag atcctcttca tctcatcggt tatctggagc
      241 gatccaatat tttagaggcg cgacttaggc aacttaacga tgaacatgtg cgcgaacatg
      301 gctggtttca taacctcttt ttgaaaatta tggcaagata atttcatgag acggaaaatt
      361 cgatcttatc gtcaggtggt gaagcggctt gaagcggttg ccagaaatga aaccgggctt
      421 caactaaaac gtctaggaaa aatcaagtcg aaccctgctg actatgacct gtatctgatc
      481 cgatcccaaa aagcaccgcg attttttcgg cggcggattt gtctctgcgc cggaatccac
      541 ggcgatgagc cggcaggcgt cgaggcgatc ctaacactga tcgaagaccg ccgccggttg
      601 agggaaatac ttgatgaact ccacctcacg atcttcccat gtatcaatcc ctatggcttt
      661 gaacacaaca cgcgtacgaa tggtctgggc ctggacctca accgtcaatt ccgaagcagg
      721 cggcctcctg tcgaggtgag tcttgtcaaa caagcgatcg gccggacctg ctacgacctt
      781 gcgatggagt tccatgaaga tgtggacacg aatggtttct atctctacga gctcaccgcg
      841 ggaaaagaac ctgcatgggg cccgctgatc gttgagaaag tcggcctgca ttggccggtc
      901 aaccgcggtg aggagatcga gggaatgaaa agcaagaacg gtgtgattca tcgaaaaaac
      961 cagaacggct cctttaggca actgatgcag acacgacctg actggcccca ggccttttac
     1021 ttcttctccc acggaacaag acactgcttc acgaccgaga cgccggtcac gctggacatc
     1081 aaagaccggg ccaaaataca tctgaccgcg cttgaggtat cgctccgaca gttgactctt
     1141 tcttaatcca gcggctcaaa caacttcttc accacgctcg actccgtgtt gctgatcccc
     1201 gtcacgctga aaaagaagct cacgtcattc ttaacaggct cgatgttatt gatccccgtc
     1261 gcttcagagt gaatatatga aaccgtgaag ccccagcagt ggcaggagcc ggtataccgc
     1321 actgctgccc tcgcttccgc aagttgaccc tggtcataat catagaagat cttaccggcc
     1381 aggctgacac gcgaggcgat gttgaccacg gctcccatgg tgaggaattt cagatccgcg
     1441 gcctgtgcct gctgtagacc caacgaaaac ggttccagca ggtcgcctcg ctgcggtgtc
     1501 gtcccggccc gtgtatagcg ttctcccacg gagaggagca gaatctcgtt caagtggaat
     1561 ttaagatcgg tatccaccgt tgtgaagcgg tgtgggttgt atacgtccag aaagctgtct
     1621 acatgcagag acagacgacg actggcccgg aggatcatct ctccacggaa gtccgaaaaa
     1681 gatctctggg gaggaagatt cagaatcctc tcccgtccat agctctgcgt aaatttaaga
     1741 tagaaaatct caaggcgctt cgtcgtcccg ccttctcccg ttcgttttaa caccagccga
     1801 ttgatcaagg aataggtcaa gaggtttttc tggggaaacg catccacctg atcgagttga
     1861 gggatatcgg tcgaatcaaa accgtggacg tattcataaa ggacattagg ttcca
//
LOCUS       DATMKH010000509         2879 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3743280, whole genome shotgun sequence.
ACCESSION   DATMKH010000509 DATMKH010000000
VERSION     DATMKH010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3743280"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="VNV63_06065"
     CDS             <1..303
                     /locus_tag="VNV63_06065"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXC62220.1"
                     /translation="VVCCEEGRGAIRRYQEARASGRPFDVVILDLTVPGEMGGKETME
                     KLREIDPKVKAIVSSGYSNDPVLADFQEYGFSGRIAKPYTMENLGKTIKKCLEKKE"
     gene            412..1461
                     /locus_tag="VNV63_06070"
     CDS             412..1461
                     /locus_tag="VNV63_06070"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXC62221.1"
                     /translation="MKGAEQGLLYQYQAALRDYLAGAGEMALAHAYELGRKAVSEGLG
                     VLEMMAIHRKTLDNVLGDAAEHGERNLVVKAEEFLAESLSPFEITHRAFREANVKLHL
                     LSEHLQSVREEERSQIARDIHDELGQALTCLKMDLSWVSSRLSTDHSLRPSPPTNPGN
                     SARRPQPKLRNGTRPKRQESLQAKTVTMLKLIDRTIQTVRRLSSELRPALLDDLGLAA
                     TIEWQMQEFKTRTGIRYKLTLPSKDLILDQKRSTAIFRIFQETLTNVVRHSNATQVSI
                     SLKKKADNLILRVKDNGKGIAENEISDAKSLGVLGMRERALLLGGEVHIAAGAHGKGT
                     TVTVRVPMGKAGGTS"
     gene            1458..2090
                     /locus_tag="VNV63_06075"
     CDS             1458..2090
                     /locus_tag="VNV63_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014260863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXC62222.1"
                     /translation="MIKILIADDHAIVRKGLKQILAETTDIIVAGEAHNGQEVLNKVR
                     TGDWDVVVLDITMPGTSGLDILKEMKNDHPKLPVLVLSIHSEDQYAVRVLKAGASGYL
                     TKESAPDELVKAIRKVSTGGKYVSAYTAEMLAYDLDRNSEKPPHEFLSDREYQVLCLL
                     CLGKTVKEIAKEKSLSIKTISTYRTRILEKTKMKTNAELIHYGIRNRLVD"
     gene            2320..>2879
                     /locus_tag="VNV63_06080"
     CDS             2320..>2879
                     /locus_tag="VNV63_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018158831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsbRD N-terminal domain-containing protein"
                     /protein_id="HXC62223.1"
                     /translation="MARRDESIAVQPTHESTTVLLRELVAHLRENRTQLREEWARRIT
                     EAGLLTAMTREEIFAEATAVYDNYVAVLETGSVEALQAYARDLSERIIARGVETHEVL
                     GIVLLLRDVLARSLFKKYQSDLGLLNRVLDAYEPAANRIANTVGVSFVQERERIIRQQ
                     QEAIRELSTPVLQVRDRLLILPIIGV"
BASE COUNT          793 a          648 c          826 g          612 t
ORIGIN      
        1 gtggtgtgct gtgaagaggg caggggtgcg atcaggcggt atcaggaagc gcgtgcctcg
       61 ggccggccct ttgatgttgt cattttggat ctgaccgtac ccggtgagat ggggggaaaa
      121 gagacgatgg agaaactgcg cgaaattgac ccaaaggtga aggctatcgt ttcaagcgga
      181 tactcgaatg atccggtact ggcggatttt caggaatatg ggttttctgg acggatcgca
      241 aagccctaca ccatggagaa tttaggcaag accataaaaa aatgtttgga gaagaaggaa
      301 tgattaattt ccctctttcc tctagatctg ctgatgttta aagccgcctt gctgtaagaa
      361 ttaaaatggt gtattctcat cgcaatcgta tccccaaaat tggactccag aatgaaaggc
      421 gcggaacaag ggcttctgta tcagtatcag gcggctttac gggactacct tgcgggggcc
      481 ggggaaatgg ctctcgcgca tgcgtatgag ctggggcgca aggccgtttc ggagggactg
      541 ggggtgttag agatgatggc gattcaccgg aagacattag acaatgtttt gggagatgcg
      601 gcggagcatg gggagaggaa tctcgtggtc aaagccgagg agtttttagc ggagagcctt
      661 tcgccgtttg agattaccca ccgcgcattc cgagaagcca atgtcaagct tcatctcctg
      721 tccgaacatt tgcaatccgt gcgggaagag gaaaggagtc agatagcgcg tgacatccac
      781 gatgagttgg gtcaagcctt gacgtgtttg aaaatggatc tgtcctgggt gagcagccgg
      841 ttatcaacgg accattctct acggccttct ccgccgacga accccggaaa ttcagcgcgc
      901 cggccccaac ccaaattgcg aaatggaacg aggcccaaaa gacaggaatc cctgcaggcg
      961 aaaacagtaa cgatgttaaa actcatcgat aggacgatcc aaacggtgcg gaggctctcc
     1021 tcggagttaa ggcccgccct gctggacgac ctgggtcttg cagccaccat cgaatggcag
     1081 atgcaggaat ttaaaaccag aacggggatc aggtacaagc tgaccttgcc ttccaaggac
     1141 ctcattctgg atcaaaagcg gtcaaccgca attttccgta tctttcagga gacattgacc
     1201 aatgtagtcc gtcactcaaa tgcaacccag gtcagcataa gtttaaagaa gaaagcggac
     1261 aacttaatat tacgcgtgaa agacaacgga aaagggattg cagagaatga aatttccgat
     1321 gccaagtctc tcggggttct tggtatgagg gaacgtgctc ttcttttagg gggagaggtt
     1381 catattgcgg cgggggcgca tggcaaagga acaacggtta ccgtgcgggt tccgatgggc
     1441 aaagcaggtg gaacgtcatg ataaagattc tcatcgccga cgatcatgcc attgtacgga
     1501 aagggctaaa gcaaatcctg gcggagacca ctgatataat cgtcgcagga gaagcacaca
     1561 acggacagga ggttttaaac aaggttcgga ccggcgattg ggatgtggtc gttctggaca
     1621 tcaccatgcc gggcacgagc ggactggata tcttaaagga gatgaagaat gatcacccta
     1681 aactccctgt cctggtgtta agcattcatt ctgaggatca atacgcagtg cgtgttttga
     1741 aggcaggcgc ctccggatat ttgacgaagg agagtgcacc ggatgaatta gtaaaagcca
     1801 tccggaaggt atccacgggc ggcaaatatg tcagtgcata tactgccgag atgttggcgt
     1861 atgacctgga cagaaattca gaaaaaccgc ctcatgagtt cctctctgat cgtgaatatc
     1921 aagttctgtg cttgctttgc ttaggcaaaa ccgttaaaga gattgcgaag gagaagtcgc
     1981 tgagcattaa gaccatcagc acatacagaa cacgcatttt agaaaaaacg aaaatgaaaa
     2041 ccaacgcgga attaatccat tacggcatac gaaatcggct cgtggattga gaacaagcgg
     2101 catttcgatc acccaaaaac ggtgtaatcg agcaagctta agtcttacaa acaaagggat
     2161 cggagtccta caaaaaaatc agacctagac cgataccgtt cctcgtcgga tcgtttatga
     2221 tctaggcagc ttttgcgcaa gcaagtgagg atcaaccgga aagaaaaaag aagaagaacg
     2281 ggagaaaaga aagaagaaag tagaaggagg aaataacgaa tggcaagaag agacgaatcc
     2341 atcgctgttc aacctactca tgaaagcacc accgtcctgt tacgtgagct agtcgctcat
     2401 ttacgagaga accggaccca actacgcgag gaatgggcga ggcgcattac tgaagcggga
     2461 ttgcttacgg cgatgacgcg ggaagagatc ttcgcggagg ccacggccgt gtacgataat
     2521 tacgtggcgg ttctggagac gggaagcgtc gaggctttgc aggcctatgc ccgtgacctg
     2581 tccgagcgaa tcatcgcgcg gggggtcgag acccacgagg tgctgggcat cgtgctgttg
     2641 ctgcgtgatg tgcttgcccg atcactattt aaaaaatatc agtcggactt aggacttctc
     2701 aaccgcgtcc tggatgccta cgaacctgcc gccaaccgga tcgcaaatac ggtgggcgtg
     2761 agttttgtcc aggagcgtga gcgtatcatc cgccagcaac aggaggccat tcgagagctt
     2821 tccacgccgg tgttgcaggt gcgtgaccgg ttgctgatcc tgccgatcat cggcgtcat
//
LOCUS       DATMKH010000510         1267 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2139213, whole genome shotgun sequence.
ACCESSION   DATMKH010000510 DATMKH010000000
VERSION     DATMKH010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1267
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2139213"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..865)
                     /locus_tag="VNV63_06085"
     CDS             complement(<1..865)
                     /locus_tag="VNV63_06085"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HXC62224.1"
                     /translation="MSVLTSQRIGVLMGGDSSEREVSLRSGGAMEAALCRRGYHVTGL
                     DVGPSVAMDLKKEGIEIAVIALHGRGGEDGAIQGLLEVMGIPYTGSGIAASAIEMNKV
                     LTKRLLEQHGLPTPRYAVLRGSVQDHLTLPLGFELPVVLKPVCEGSTIGVSIVREERD
                     FSSALEQAFSYGPMVLVEDYIDGQEATVGILEEEPLPVIEIVPKEEFYNYEAKYTPGK
                     TEYILPARLPLATYREVQQLAVNVHQAIGCDGYSRVDIRVDRKFRPFVLEINTLPGMT
                     ESSLLPKAAKEA"
     gene            complement(871..>1267)
                     /locus_tag="VNV63_06090"
     CDS             complement(871..>1267)
                     /locus_tag="VNV63_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylenolpyruvoylglucosamine reductase"
                     /protein_id="HXC62225.1"
                     /translation="IKFGYRSSRLPPGVILGGWLKLKNAKTGEIRERINRYMKRRNAT
                     QPIHLPNAGSIFKNPPGDHAGRLIETAGLKGMRIGDAQISDKHANFIVNRGKATAHDV
                     LDLIKLIGRTVEDQTGITLELEIRIVGKD"
BASE COUNT          279 a          397 c          297 g          294 t
ORIGIN      
        1 ccgcctcctt cgccgccttg ggcagaagac ttgactcggt cataccgggc aaggtattaa
       61 tctccaaaac aaacggtctg aattttcgat ccacacgtat gtccacgcgg gagtagccgt
      121 cgcaaccaat cgcctgatgg acattgaccg caagctgttg aacttcccga taggttgcaa
      181 gcggcagacg tgccggcaaa atatactcgg tcttacccgg agtatacttc gcctcgtaat
      241 tgtaaaactc ttccttcggc acaatctcga tcacgggcaa cggttcctct tccaaaatcc
      301 caaccgtggc ctcttgcccg tcaatataat cttcgaccaa taccatcggc ccgtacgaga
      361 aagcctgctc cagggccgat gaaaaatctc gttcttctcg aacgatactc acgccgatcg
      421 tggagccttc acacacaggc tttaacacga ccggcaactc aaacccgagc ggcagggtta
      481 aatgatcctg gacggatccc cgcagcaccg catagcgtgg ggttggaaga ccatgttgct
      541 ccaacaatct ttttgtcagg accttgttca tctcaatcgc actggccgca atccccgaac
      601 cggtatacgg aatacccatc acctctaaaa gaccctggat tgcaccgtct tcacccccgc
      661 ggccgtggag cgcgatgacg gcgatctcga tcccttcctt ttttaaatcc attgcgaccg
      721 acggacccac atcaagaccc gtcacatgat agccccggcg gcataatgcc gcttccatcg
      781 ccccaccgct tcgaagcgag acctcccgct ccgaggagtc gcctcccatg aggacaccga
      841 tcctctgact ggtcaagact gacatgattc ttagtctttc cccacaatcc ggatctccag
      901 ctccaatgta atcccggtct gatcttcgac cgttctcccg atcagtttga tcaggtccaa
      961 gacatcgtgt gccgtggctt tgccgcggtt gacgataaaa ttggcatgct tgtccgagat
     1021 ctgggcatcc ccgatgcgca tccccttcaa ccctgccgtc tcgatcaacc ggccggcatg
     1081 atcacccgga gggtttttaa agatcgagcc tgcattgggc agatggatcg gttgagtggc
     1141 gttccggcgt ttcatgtatc ggttgattcg ttccctgatc tcgccggtct tcgcattttt
     1201 taactttagc caaccgccta agatgacccc tggggggagg cgtgaggaac ggtatccgaa
     1261 cttgatc
//
LOCUS       DATMKH010000511         2115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_239710,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000511 DATMKH010000000
VERSION     DATMKH010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239710"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="VNV63_06095"
     CDS             <1..681
                     /locus_tag="VNV63_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HXC62226.1"
                     /translation="INTSEDKIVTIEDPVEYELRGVVQIPVNEKKGLTFARGLRSILR
                     HDPDKIMVGEIRDAETAHIAIQSALTGHLVFTTVHANNAFDVIGRFVNMGIEPYNFVS
                     SLNCILAQRLVRTICPDCKESVVFTQEMADDSALDFAKYKAQPLSQGRGCPECNGTGF
                     KGRHAITEFLDLSDHIREMILDRRPPSEIRKTAVSEGMTTLRQSALEKVFRQETTLRE
                     INRVTFIA"
     gene            690..1607
                     /locus_tag="VNV63_06100"
     CDS             690..1607
                     /locus_tag="VNV63_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62227.1"
                     /translation="MRSLWPTRYGIYFDTGRVAVAEIEKHGGRLKLKNHEIAYLTDRV
                     LIPDPLTPGIRDAELVQKTLRAMLKPGRRPKAVSVSLSDILAKVMLIPMEKPITERAD
                     VEKLIQWKIEKSTLYQPQGLRLRYQLLPSLLASSIHEQVLLQYESVLQASGLEPRLID
                     IASFHVFNLYHDYIFQKSAPQHRFILIYAGDSFSTVMIFTEGVLNFIRVKALRSQPRS
                     AEDRVAEELRSSIDFYSEDHDITSITHLFAVGLPISEMLSKSWGDSFPWEIVQLKPED
                     VISITSQNGEIPEKDWPTLIPAIAAAVRR"
     gene            1615..>2115
                     /locus_tag="VNV63_06105"
     CDS             1615..>2115
                     /locus_tag="VNV63_06105"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HXC62228.1"
                     /translation="MKFQTDFASHEYRYIYGLQGIFIIASLLLVLLAVWNFLNYRAAP
                     QEADRVTGSLKRVQEQSAQLASKLQVNGKALTPDQITALSKEVAFANELLQRKTFFWS
                     SLLSDIEGVIPDNIALTRIQLDFKEQRVMLTGSAVSLKDLTQFIIRLEDSPAFKDVFL
                     ENQKTGE"
BASE COUNT          477 a          572 c          568 g          498 t
ORIGIN      
        1 atcaatacct cggaagacaa aattgtcacg atcgaggacc cggtggagta cgagctccgg
       61 ggtgtggttc agattccggt caacgaaaag aaagggctta cctttgcacg gggcctgcgc
      121 tcgattctga ggcacgatcc ggacaaaatc atggtcggcg agatccgcga cgccgagacg
      181 gcacacatcg cgattcagtc cgctctgacc ggccatctcg ttttcaccac ggtacacgcc
      241 aacaacgcct ttgatgtcat cggccgtttc gtcaacatgg ggatcgagcc ttacaatttt
      301 gtttcgtcct tgaactgcat cctggcacag cgtctcgtcc gaaccatctg ccccgattgt
      361 aaagagtccg tggttttcac acaggagatg gcggatgatt cggctctgga ttttgccaag
      421 tacaaggcgc aaccgctttc tcagggtcgc ggctgtcctg aatgcaacgg aaccggtttc
      481 aagggccgcc acgcgataac cgagttcctg gatctgtcgg accatattcg ggagatgatc
      541 ctggaccggc gtccgccgtc ggagatccgg aagaccgctg tttcggaagg catgacgacc
      601 ttgagacagt ccgccctgga gaaggttttc cggcaagaaa ccacgctccg ggagatcaac
      661 cgggtgacct tcatcgcata gggagtggga tgagatctct ttggccgacg agatatggca
      721 tctattttga tacgggtcgc gttgctgtgg ccgaaatcga aaagcacggc gggcgcttaa
      781 agttgaagaa ccacgagatc gcgtatctta cggatcgcgt cttgatccct gatccgctta
      841 cacccggcat ccgggatgcg gaactggttc aaaagacatt gcgggcaatg ttgaagccgg
      901 gccggcgtcc caaggcggtc tcggtgagcc tttcggatat cctcgcgaag gtgatgctca
      961 ttccgatgga aaaaccgatc accgagcggg cggatgtcga gaaattgatc caatggaaaa
     1021 tcgaaaagag cacactgtat cagccccagg ggttgaggct gcgttatcag ttgctcccgt
     1081 cgcttctggc atcctcgata catgagcagg tgcttcttca gtacgagtcg gtcctccaag
     1141 ccagcggcct ggaacccaga ctgattgaca tcgcctcctt tcacgtcttc aacctctatc
     1201 acgattatat ctttcaaaag tccgcaccgc agcaccgctt cattcttatt tacgcgggcg
     1261 actcattttc caccgtgatg atcttcactg agggcgtctt gaacttcatt cgcgttaagg
     1321 cgcttcgctc ccagcccaga tcggccgagg acagagtcgc tgaggaactg cgttcttcca
     1381 tcgattttta tagcgaagat catgacatca cctccatcac acatttattt gcggtgggac
     1441 tcccgatctc ggaaatgctt tcaaaatcat ggggagactc ttttccgtgg gagattgtcc
     1501 agcttaaacc ggaggatgtt atttcaatta cctcgcagaa cggggagatc ccggaaaagg
     1561 actggccgac attgatcccg gccatcgcgg cggccgtgcg gagatgaatc ttttatgaag
     1621 tttcaaactg atttcgcctc acacgagtat cggtatatct acggactcca ggggatcttc
     1681 attattgcat cgctccttct cgttctgctg gcggtctgga acttcttaaa ctatcgcgcc
     1741 gccccgcagg aagccgaccg tgtgacaggc agtctcaaac gggtgcagga gcagtcggct
     1801 caacttgcgt ccaagctgca ggtcaatgga aaagcgttga ctcctgatca gataaccgct
     1861 ttgtcaaagg aggtcgcgtt cgccaacgag ctgctccaga gaaagacctt tttctggtcg
     1921 agtttgttat cggatatcga gggggtgatt cctgataaca tcgcgttgac ccgcatccag
     1981 cttgatttca aggaacagcg cgtgatgctg accggctccg cggtttctct caaagatctc
     2041 acccagttta tcatccggtt ggaggattca cccgccttca aagatgtgtt tcttgaaaac
     2101 cagaaaaccg gcgag
//
LOCUS       DATMKH010000512         1877 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1203754, whole genome shotgun sequence.
ACCESSION   DATMKH010000512 DATMKH010000000
VERSION     DATMKH010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1203754"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="VNV63_06110"
     CDS             <1..654
                     /locus_tag="VNV63_06110"
                     /inference="COORDINATES: protein motif:HMM:NF020752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DALR domain-containing protein"
                     /protein_id="HXC62229.1"
                     /translation="EIFEKSNWPDPVTAEVLRYFLVSTHYRSPIDFSNEALETAKAGL
                     DKFYILFQKLDEVKITKGTRDAQLKKSMKVFASSFEKAMDDDFNTALAISEMQRLRSA
                     VNTVLDKGLSKSLSASVLKLFRKYGSILGLFTLSTAQWNSIAGAGEFKTSSSNGRESD
                     GGVPPSLSPADIERLIQERNEARQTKDWERADAVRKQLSDAGIILEDRPDGTTRWRR"
     gene            642..1421
                     /gene="rlmB"
                     /locus_tag="VNV63_06115"
     CDS             642..1421
                     /gene="rlmB"
                     /locus_tag="VNV63_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003392024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HXC62230.1"
                     /translation="MEKVTSKDTASGADGQEILFGVHPIQEALASGKRLIHKLYLLRG
                     RNDKPVQAILKSAKDQGVAIYFESRDVLDRLAGSTRHQGVLGFLGAASYATLEKLLQM
                     AQDRSEPPFLILLDGVEDPRNLGAIIRTAEAVGAHGLVIPQRRAAGVTAAAARAAAGA
                     EEFLPVAQVVNLTQTIQDLKTRGLWIYGLDEKAKQVYHQTDLRGPLALIIGGEGKGIR
                     RLVLEHCDALISIPMVGRVESLNVSVAAAIVLYEVLRQRRK"
     gene            complement(1509..>1877)
                     /locus_tag="VNV63_06120"
     CDS             complement(1509..>1877)
                     /locus_tag="VNV63_06120"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXC62231.1"
                     /translation="YSIGRTGVGAAGMFLTSLLIYSGTVAKDPYTTILMLSLGAGILY
                     ATVGAYWASTIDFSHKHSGALSGLMNMGANLGGTISPTLTPFIGEKWGWPVALTVAAG
                     AAFLGSLLWLGVKFDKARKM"
BASE COUNT          478 a          472 c          506 g          421 t
ORIGIN      
        1 gagatctttg agaaatcgaa ctggccggat cccgtcacag ccgaagttct tcgttacttc
       61 cttgtttcga cacattatcg cagcccgatt gatttttcaa acgaagctct tgaaactgcc
      121 aaggcagggc tggataagtt ctacatcctt tttcagaagc tggatgaagt aaagattaca
      181 aagggtacga gggatgcgca actgaagaag agtatgaaag tctttgcatc ctcttttgaa
      241 aaggcgatgg atgacgactt taacaccgct ctggccatca gcgagatgca gcggcttcgg
      301 tcagcggtca atacggtatt ggataaagga ttatcgaagt ccctctcagc gagtgtcctc
      361 aaactcttcc ggaaatacgg ctcgatactg ggtctcttca cgctttcgac cgcacaatgg
      421 aattccattg caggtgccgg cgaatttaaa acttcgtcgt caaatggaag agaatcggat
      481 gggggggtgc ccccaagtct ttctccagcc gatattgaac ggctgatcca agagcgaaac
      541 gaagcccgtc agacaaaaga ctgggagaga gccgacgctg ttcgtaaaca actgagcgat
      601 gcagggatca ttctcgaaga ccgcccggac gggacgaccc gatggagaag gtgacatcaa
      661 aagatactgc cagcggggca gacgggcaag aaatcctctt cggcgtccat cccatacagg
      721 aggccttggc ctcgggaaag cgtctaatcc acaaactcta tctcctgcgc gggcgtaacg
      781 acaaaccggt tcaagcaatc cttaaatcag ccaaggacca aggggtcgcg atttattttg
      841 aatcgagaga tgttttagat cgtcttgccg gcagcacgcg tcaccagggc gttctgggat
      901 tcctgggcgc cgcatcctat gcaacgctcg agaagttatt acaaatggca caggaccgaa
      961 gcgagccgcc atttttgatc ttgttagacg gtgtggaaga tccgagaaac ctgggtgcaa
     1021 ttattcgaac tgccgaagcg gtgggcgcgc acgggctggt tatcccgcag cgtcgggcgg
     1081 ccggggtcac agctgccgct gccagggccg ccgcgggggc ggaagaattt cttccggtgg
     1141 ctcaggttgt caatctaacg cagacaattc aggatctgaa gacgaggggt ctttggatct
     1201 atgggctgga tgaaaaagcc aaacaggttt atcatcaaac ggacctgaga gggccgctgg
     1261 ccctgattat cgggggagag ggaaaaggaa tacggcggct cgttttggag cattgcgacg
     1321 cgttgatctc gatccctatg gtcggccgcg tcgagtcgct caatgtctcg gttgccgcag
     1381 ccatcgtgct atacgaggtg ttacggcaga ggcgaaaata agaagaatcc agtttttatc
     1441 ttttagcatt taggttcttc tccccgcgta tgttggttct ctcagtttac gactaacaaa
     1501 aatcgctttc acattttcct tgctttatca aatttaactc caagccaaag caggcttccc
     1561 aaaaaagctg cgcctgctgc cacggttaaa gcaacaggcc atccccattt ttcaccgatg
     1621 aaaggcgtca gtgtcgggga aatcgtcccg cccagattgg ctcccatatt catcaagccg
     1681 gacagggcac cggaatgttt gtgagagaag tcgatcgtgc ttgcccaata agcacccacc
     1741 gtcgcataaa gaattccggc gcccaaagag agcatcagga tcgtggtgta aggatcctta
     1801 gctaccgtgc cactataaat gagcaaagat gtcagaaaca ttccagcagc gccgacgcct
     1861 gttctcccta tcgaata
//
LOCUS       DATMKH010000513         1279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2805718, whole genome shotgun sequence.
ACCESSION   DATMKH010000513 DATMKH010000000
VERSION     DATMKH010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2805718"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1279)
                     /locus_tag="VNV63_06125"
     CDS             complement(<1..>1279)
                     /locus_tag="VNV63_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016778878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HXC62232.1"
                     /translation="VTRSDGLDERDLTPFEGVRAELIDDLEEHPTDLILGLNKRNKEV
                     FDAYRLDVETGELKLVAENPGNIGTWTTDHDGNIRVATTTDGVNTSLLYRKNENDSWK
                     TVITTSFKESFRPQFFTFDNKALYGVSNVGRDKAAVVEFDPETGKETKTLFEQPEVDV
                     SGLNYSRKRKVITFASYTTWKQGRQFFDPESEGLYKALSEKLPGYDVYVVSSNKEEDM
                     FIVRTITDRSLGAFYLFDQKADDLTKLADRNPWLKEEQLSEMQPIEYTSRDGLTIHGY
                     LTLPKGKPVKNRPMVVNPHGGPWVRDEWGFNPEVQFLANRGFGVLQMNFRGSTGYGRK
                     FWEAGFRQWGESMQDDITDGVKWLVDQGIADPERVAIYGGSYGGYAVLQGLTKTPDLY
                     AAGVDYVGVSNLFTFMKTVPPYWKPFLEMMYEMV"
BASE COUNT          278 a          354 c          308 g          339 t
ORIGIN      
        1 accatttcat acatcatttc caagaaaggc ttccaatagg gagggaccgt tttcatgaag
       61 gtgaacaggt ttgaaacacc gacgtaatcg actcctgcgg cgtacaaatc gggtgtcttg
      121 gtcagacctt gaaggacggc atagccacca tagcttccac cgtaaattgc cactcgctca
      181 ggatcagcga tcccttggtc aaccaaccat ttcaccccat cggtgatatc gtcctgcatg
      241 ctttctcccc attgtcggaa gcccgcctcc cagaacttac gaccatagcc ggtcgaaccg
      301 cggaaattca tctgcaaaac gccaaaaccg cggttagcca ggaattgtac ctccgggtta
      361 aatccccatt catcccgcac ccatggtccg ccgtggggat tgaccaccat cggccggttc
      421 ttcactggtt tgccttttgg taacgtgaga tatccgtgga tcgtaaggcc atcccgagac
      481 gtgtattcga ttggctgcat ctcggataat tgctcttctt tcagccacgg attgcgatct
      541 gcgagcttcg tcaagtcgtc ggctttttgg tcgaaaaggt aaaacgcccc cagtgaccga
      601 tccgtaatgg ttcgaacaat gaacatgtct tcctctttat tggaagagac gacatagacg
      661 tcataaccgg gcagtttctc gctgagagcc ttgtaaagcc cctcagattc cggatcaaag
      721 aattgccggc cttgtttcca ggtggtgtac gacgcgaagg taatcacttt tcgttttctg
      781 gaatagttta gcccggagac atccacttcc ggctgttcga acagcgtctt cgtctctttt
      841 cctgtttcag gatcgaactc gaccaccgcg gctttgtctc ggccgacgtt cgaaacaccg
      901 taaagagctt tgttgtcgaa ggtgaaaaac tgagggcgaa agctttcctt gaagctggtc
      961 gttatgacgg ttttccagga atcgttttcg ttcttgcggt atagcaaact tgtattgaca
     1021 ccgtcggttg tcgtggcaac tcggatgttt ccgtcgtgat cagttgtcca ggtcccgatg
     1081 ttccccggat tttcggcgac cagcttcaat tcgccggtct cgacgtccag ccggtaagcg
     1141 tcgaacactt ctttattccg tttgttcagg ccgaggatca gatccgtcgg atgttcttcc
     1201 agatcatcaa tcaactcggc tctgacacct tcaaacgggg tcaaatcccg ttcgtcgagc
     1261 ccgtcactcc gggtcacgt
//
LOCUS       DATMKH010000514         1331 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_557696,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000514 DATMKH010000000
VERSION     DATMKH010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557696"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="VNV63_06130"
     CDS             complement(<1..445)
                     /locus_tag="VNV63_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HXC62233.1"
                     /translation="MKAIQITQTGGPEVMSIREVPTPVPGPGEVLIRVAASGVNFIDL
                     YVREGRYANPVPFIPGQEASGTIAAVGEGVRLIKEGDRVAWCSILGTYAEFALAPANR
                     VVPIPADISFDQAAAVMLQGMTAHYLSHSAYPIRKGDEILIHAGAG"
     gene            complement(500..1072)
                     /locus_tag="VNV63_06135"
     CDS             complement(500..1072)
                     /locus_tag="VNV63_06135"
                     /inference="COORDINATES: protein motif:HMM:NF028169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXC62234.1"
                     /translation="MKRLRPEEIEAVIINTVFEDLQEHGFRAVTVESISAKTGVAKTS
                     IYRRWPNKAAMVMEAFLFRIGPGIEFPRRSSYVESIRLQMLALAKAFRGPFGSMIKAL
                     LGEAQFDAELAEAFRTRWITPRREAAKEVIRSAIRNKELRSDVDLDQALDALYGWLYY
                     RLMIGSGPLSDACVREIFSAVLDGLRVRKD"
BASE COUNT          318 a          367 c          345 g          301 t
ORIGIN      
        1 cacccgcgcc cgcgtgaatc agaatctcat cccctttccg gatggggtac gcagagtggg
       61 acaagtagtg cgccgtcatc ccctgcagca tcaccgccgc tgcctggtcg aagctgatgt
      121 ccgcgggaat gggtacgaca cggttggcag gagccaatgc aaactccgcg tacgttccga
      181 ggatgctgca ccaagcgacg cgatctcctt ctttaatcaa tctcacccct tcgccaaccg
      241 ccgctatcgt gccggaggcc tcctgtccag gaatgaatgg gaccggattg gcataacggc
      301 cttcccgaac atagaggtcg atgaagttca cgccgctggc cgctacccga atcaacacct
      361 cgccaggacc aggaacaggt gttggaactt cacgaatgct catcacttca gggccgccgg
      421 tttgtgtaat ctgaattgct ttcacctgag ataacagtcc tttttgagat aactatggag
      481 aggaatgatc ttggggctcc taatccttcc gaacgcgcaa accgtccagt acagcggaaa
      541 atatttctcg cacacaggcg tcggagaggg gccctgatcc gatcatgagc cggtagtaaa
      601 gccatccata gagcgcatcg agcgcctggt ccagatcgac gtcagagcgt aattccttat
      661 tccgtatggc agatcgaata acctctttcg cagcctctcg tcggggagtg atccaacgag
      721 tgcgaaaggc ctcagccaat tccgcatcga actgggcttc gccaaggagc gccttgatca
      781 tcgagccgaa gggtcctcgg aatgccttgg ccaaagccag catctggagg cgaatgcttt
      841 ctacataact ggacctcctg ggaaattcga ttcccgggcc gattctgaag aggaaagcct
      901 ccatgaccat agcggccttg ttgggccagc ggcggtaaat ggatgtcttc gctacacccg
      961 ttttggcaga gatggattca acggtaactg cgcgaaaacc gtgctcctgg aggtcctcga
     1021 agacggtgtt gatgatcaca gcttcaattt cttcaggacg aaggcgtttc acagaccttc
     1081 tatccttctc gatacgcttc gacatccatc cccacttctg gcctccccgc aaatcgcagt
     1141 gatcgatcaa ttactcgtaa ctatagatgc tacgatacgg tgcgtagctt aaaaaaaagt
     1201 gctgatttgt tacggatggg gaaattggat cggagcttcg tctgcaacat acggatagaa
     1261 gatgtatagc cagattagaa cttgtgatac ctagtccggc agtcataagc ctgcattatg
     1321 attaatgtct a
//
LOCUS       DATMKH010000515         1022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1523582, whole genome shotgun sequence.
ACCESSION   DATMKH010000515 DATMKH010000000
VERSION     DATMKH010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1523582"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VNV63_06140"
     CDS             complement(<1..260)
                     /locus_tag="VNV63_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009796643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HXC62235.1"
                     /translation="MTVQNDQKVQLVRMEIGGKEISFETGRMARQADGAILARCGDTV
                     VLATTVSAKQPREKIDFLPLVVDYQEKAYAAGKIPGGFFKREG"
     gene            complement(265..534)
                     /gene="rpsO"
                     /locus_tag="VNV63_06145"
     CDS             complement(265..534)
                     /gene="rpsO"
                     /locus_tag="VNV63_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HXC62236.1"
                     /translation="MALLKEAKQRLIGEHQLHKKDTGSSDVQIAVLTRQINSLSEHFK
                     IHKKDQHSKQGLLKMISRRRKLLDYLKRCDLGRYKKIIQTLEIRK"
     gene            complement(546..>1022)
                     /locus_tag="VNV63_06150"
     CDS             complement(546..>1022)
                     /locus_tag="VNV63_06150"
                     /inference="COORDINATES: protein motif:HMM:NF013626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PUA domain-containing protein"
                     /protein_id="HXC62237.1"
                     /translation="MLSFENQDATFDVVCSKGTYIRSLCADIGQRLGCGAHLLRLERR
                     RSGPFWIKDAISIAALEELVASGKTDRRLYSLDLVLSGFPIVRVSDQAALKCCQGVSP
                     SRSGILELPEGLSGGDLVRIHDPAGRLIAIGKTVMDRGEAESDTIEYSIKIEKVLI"
BASE COUNT          222 a          295 c          244 g          261 t
ORIGIN      
        1 ccttcgcgct tgaagaatcc gcccgggatt ttcccggcgg cataggcctt ctcctgataa
       61 tccacaacca agggaagaaa atcaattttc tccctgggct gttttgcaga caccgtggtt
      121 gccaaaacca ccgtgtcgcc gcaccgtgcc aggatcgcac cgtcggcttg ccgtgccata
      181 cggccggttt cgaacgagat ctccttcccg ccgatctcca tccggacgag ttgaaccttt
      241 tgatcgtttt gaactgtcat tgctttattt ccttatttct aaagtttgaa tgatcttctt
      301 ataccgtcca agatcgcaac gctttagata gtctaacagt ttgcgacgcc tgctgatcat
      361 cttaagcaag ccttgcttcg aatgctgatc cttcttgtgt atcttaaaat gctcgctcag
      421 cgaattgatc tggcgggtca gaaccgcgat ctgtacatcg gatgatccgg tatccttttt
      481 gtggagctga tgctcgccga tcaaacgctg ctttgcttct ttcaataatg ccatgagtcc
      541 tccatttaga taagtacctt ttctatttta atactgtatt cgatcgtgtc gctttccgcc
      601 tctcctcgat ccatcaccgt cttgccgatg gcgatcaacc tccccgccgg gtcatgaata
      661 cgcaccagat ctccaccgct taaaccctcc ggcagctcaa ggatgccgga tcgggagggc
      721 gaaacaccct ggcagcattt cagtgcggcc tgatcgctta cacggacgat cgggaagccg
      781 gaaaggacaa ggtccagaga atagagccgg cgatccgttt taccggaagc gacgagttct
      841 tccagcgcgg cgattgagat cgcatccttg atccaaaacg gcccggaccg gcggcgctcc
      901 aatcgcagaa gatgcgcgcc gcaacccaat cgctgtccga tatcggcaca gaggctccgt
      961 atataggtcc ccttggagca gacgacgtcg aatgttgcgt cttggttctc aaaagagagc
     1021 at
//
LOCUS       DATMKH010000516         2119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2806772, whole genome shotgun sequence.
ACCESSION   DATMKH010000516 DATMKH010000000
VERSION     DATMKH010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2806772"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="VNV63_06155"
     CDS             complement(<1..601)
                     /locus_tag="VNV63_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HXC62238.1"
                     /translation="MILRFLFTVWIVLGFLVRAAALQAAPIQQFMLANGLKVILLEEH
                     KAPVVTFQIWYKVGSRNEITGKTGIAHLTEHMMFKGTTHRGKGEFSKTVAVNGGTENA
                     FTDRDFTAYFENFSADHLDLSLELESDRMTNLLVNPKEFQLERDVVKEERRLRTEDDP
                     QSFVIEQMYAMAFLAHPYHNPVIGWMNDIDHLTRDDVYNF"
     gene            1068..1241
                     /locus_tag="VNV63_06160"
     CDS             1068..1241
                     /locus_tag="VNV63_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62239.1"
                     /translation="MNFNSVMLSGFVAWVIWIFVHILLLIGFRNRFVVFFDWAIYVSF
                     QRSARLITGGREK"
     gene            1255..>2119
                     /locus_tag="VNV63_06165"
     CDS             1255..>2119
                     /locus_tag="VNV63_06165"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXC62240.1"
                     /translation="MKIRQPVFAVLYVTLFLNLLVGCAEAADPLPDTQTHILKNGFRN
                     TNPAFTPPSTWVGLKFIRSRLWAMTFHPRSTNLPRIENDGSELKNNRTAATVTWIGHS
                     TLLIQLEGVNILTDPQWSNRASPVNFAGPKRLIPPGVAFENLPPIDLVLISHDHYDHL
                     DVETVRRLAQIHHPLFLVPLGLKAWFAGIGITEVEELDWWESRRIKTLTMTCLPAQHF
                     SGRSLWDRNQRLWSSWAVVGDTKRIFFAGDTGYYPVFKEVGNRLGPFDLAAIPIGAYL
                     PRVMMKMTHLTP"
BASE COUNT          473 a          535 c          534 g          577 t
ORIGIN      
        1 agaagttata aacatcgtcg cgtgtgagat gatcgatgtc gttcatccag ccgattacgg
       61 ggttatgata cggatgagcc aggaaggcca tcgcgtacat ctgctcgatc acaaacgact
      121 ggggatcatc ctcggtccgg agtcgcctct cttctttgac gacgtcgcgt tccaactgga
      181 actctttcgg gttgaccagg aggtttgtca tccgatcgga ctccagctcg agggaaaggt
      241 ccagatgatc cgccgaaaaa ttttcgaagt aggccgtgaa gtcccgatct gtgaaggcgt
      301 tctcggttcc gccgttcacc gctacggtct tagagaattc tcccttgccg cggtgggtcg
      361 tgcctttgaa catcatgtgc tcggtgaggt gagcgatgcc ggttttccct gtgatctcgt
      421 tgcgcgatcc gaccttgtac cagatctgaa aggtgaccac cggggccttg tgctcttcaa
      481 gcaggatcac cttgagaccg ttggccagca tgaactgctg tatcggcgcg gcttgtagag
      541 cagccgcccg tacaagaaac cctagcacga tccaaactgt aaacaaaaag cgtaagatca
      601 ttattggcac cgccagttaa attttttcca tcgtagcaat tgagcagtag ccttgtcaag
      661 gcggttgtat cacgcaggct ctaattttaa ttgccttcca ccgcaccggc cccgcacgcg
      721 taagtttcaa aaataaatct gatgagggtt ttctttcttt tcctgcgatg tctattgtca
      781 aatatgtaga cctaataccg attttttgaa agctctcatt aaactggata gatgggtttc
      841 ttactgagaa aataagtctc aggttatgct gttctatttc ttgtggcttt caccttcgaa
      901 gcgttaacct tgaaactcct gaaagatttt gattgcggga tataatcatt gacaattgtc
      961 ctctgatcga caaatcttta catcataata atattgacaa aagggttaat ttaatagatg
     1021 attgctaaaa tataatttat atcaatttat tgcgggccgc ggctgtagtg aacttcaact
     1081 cggttatgct ctccggtttt gtagcctggg tcatctggat cttcgtgcac atccttcttt
     1141 tgatcggttt caggaaccgg ttcgtcgttt tctttgactg ggcgatttat gtgagctttc
     1201 agcgctccgc tcgactgatt actggcgggc gggagaaatg acgagggcag caggatgaag
     1261 atccgtcagc cagtattcgc ggtgctttac gtgacactgt tccttaacct cctggtcggt
     1321 tgtgctgaag ccgccgatcc gctgcctgac actcagacgc acatcctgaa gaacgggttc
     1381 cgaaacacca atccggcgtt tactccgccc agcacatggg taggtctgaa gttcatcagg
     1441 tctcgattat gggcaatgac ctttcatcca cgatccacga acctgccccg cattgagaat
     1501 gacggcagcg agttaaagaa caatcgcacg gccgcgaccg ttacttggat cggccattcc
     1561 acactcctca ttcaactcga gggcgtcaac atcctgactg atccccagtg gtctaatcgc
     1621 gccagccctg tcaactttgc cggtccgaag cgtctgatac ctcccggagt ggcatttgag
     1681 aatctgccgc ctattgatct ggtccttatc tctcatgatc actatgatca tctcgatgtg
     1741 gaaacggttc ggcgccttgc gcagatccat caccccttgt tcctcgttcc gctcggatta
     1801 aaggcatggt ttgcggggat tggaataacg gaggtggagg agctggattg gtgggagagc
     1861 cgacgcataa aaacgctcac gatgacctgt ctccctgctc agcatttttc gggtcgtagc
     1921 ctttgggatc ggaaccaacg gctctggagc tcgtgggctg tggtaggaga cacgaagcgc
     1981 atctttttcg ctggggacac aggctactat ccggtcttca aggaggttgg taaccgtctg
     2041 ggtcctttcg acttagcagc catcccaatc ggtgcctatc ttccacgtgt gatgatgaaa
     2101 atgactcatt tgacgcctg
//
LOCUS       DATMKH010000517         1009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1523650, whole genome shotgun sequence.
ACCESSION   DATMKH010000517 DATMKH010000000
VERSION     DATMKH010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1523650"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="VNV63_06170"
     CDS             <1..373
                     /locus_tag="VNV63_06170"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXC62241.1"
                     /translation="HKLLGAGARGYILKEDAGRDLITAVKALLANKTFFTPKVAQKVR
                     DGHLGKVPKADEEFSPITARQREVLQLLAEGKSSKEVANILGITFKTAETHRSNLMRR
                     LNCHSVMELVRYAIRNHIVEA"
     gene            complement(370..558)
                     /locus_tag="VNV63_06175"
     CDS             complement(370..558)
                     /locus_tag="VNV63_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62242.1"
                     /translation="MAKDKRTIGVDVSEEVVTAFEAGIVFGRTVEIRRPKRHPLWSDD
                     EWLQALIKAVGKKAESKG"
     gene            complement(697..>1009)
                     /locus_tag="VNV63_06180"
     CDS             complement(697..>1009)
                     /locus_tag="VNV63_06180"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXC62243.1"
                     /translation="CSLLADQSDWDVYEAESGKEALHRIQEVEPDVVVLAVLMTEMNG
                     LETARKIRKLAPETRVIFISSQYTQQEAALITRLFGSNFVQKSDLSTELIPTISRLLP
                     C"
BASE COUNT          242 a          256 c          272 g          239 t
ORIGIN      
        1 tcacaaactc ctgggtgcgg gggcgcgcgg atatatcctc aaggaagatg caggccgtga
       61 cttgataact gccgtgaagg cgctgctggc gaacaaaacc ttctttacgc caaaggtggc
      121 gcagaaggtg agggatggac atttaggcaa ggtgccaaag gcggatgagg aattctcacc
      181 gataacggcg cggcaaagag aagtcctgca gcttttggcg gagggcaaga gcagcaagga
      241 agtcgcaaac attctgggca tcaccttcaa gacagccgaa acgcaccgat cgaacctgat
      301 gcggaggctc aattgtcatt cggtcatgga gctagtccgc tacgctatcc gcaatcacat
      361 cgtggaggct taacctttgg attctgcttt cttcccgacc gctttgatta gggcttgaag
      421 ccattcgtca tctgaccaca acgggtggcg ctttggccgt ctaatttcca ccgttcgtcc
      481 gaacacgatc ccggcttcaa atgcggttac cacttcttcc gatacatcta ccccgattgt
      541 ccttttatcc ttagccatcc cgcctcccaa tccgattcta acgccgtgga gagcccgcag
      601 tgtctcctat tagacaaagc agtgtttcgt attggacaaa aaagaaataa gggccctaac
      661 tcttctggga aagagatttg gggggtaagg agtcatctag catgggagaa ggcggctgat
      721 tgtagggatc aattccgtac tgaggtcaga tttctggacg aaattactgc cgaagaggcg
      781 ggtgatgagt gccgcctctt gttgagtgta ctgactgctt ataaagatca ctctcgtttc
      841 aggtgcgagc ttgcgtatct tacgagctgt ttcaaggcca ttcatttcag tcatcagaac
      901 agccagcacg acgacatcgg gttcgacttc ctggattcgg tgaagagcct ctttgccgct
      961 ttcggcttca tagacatccc aatcggactg gtcggccaga agagagcac
//
LOCUS       DATMKH010000518         1143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3117588, whole genome shotgun sequence.
ACCESSION   DATMKH010000518 DATMKH010000000
VERSION     DATMKH010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3117588"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1093
                     /locus_tag="VNV63_06185"
     CDS             <1..1093
                     /locus_tag="VNV63_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXC62244.1"
                     /translation="TVLCPRGKGYSQGHEVIEGVHIFRHPAPKEGNGPLGYLYEYGCA
                     LLWEFLYAWWIYLRRGFHVIQGCNPPDDIFLVALPFKLLGVKYIFDHHDANPELYLSK
                     YEKKGTFYNIQVWLEKMTYRVSDVVMATNSSYRDLAITRGGLAPEDVFIVRNGPDLGS
                     FKAVPPNAALKYGKPYLVGYVGTMSVQEGLDILLDVAAHVKNLGRRDIHFTCVGGGPG
                     LAALKKMAQDKNLEEMVNFTGRVPDRELLEILSTADVCVNPDKPCRMNDISTMIKIME
                     YMALGKPIVQFDLKEGRFSAKEASLYSDDGNQVNDFANKVLWLIENPYERKKMGEFGQ
                     RRVREELAWDYSVENLLAAYRRAFSKRPS"
BASE COUNT          279 a          283 c          324 g          257 t
ORIGIN      
        1 gaccgtgttg tgtccgcgcg gcaagggcta ttcacaaggg cacgaggtaa tcgaaggtgt
       61 ccacatcttc cgacaccccg ctcccaaaga aggaaatggg cctctcgggt atttgtacga
      121 atacggctgc gcgctgcttt gggagtttct gtacgcgtgg tggatttatc tgcggcgtgg
      181 ttttcacgtg attcagggct gcaacccacc cgatgacatc tttctggtcg ccttaccgtt
      241 taaactcctg ggggtcaagt acatcttcga tcatcacgac gccaacccgg agttgtatct
      301 ttcgaaatac gaaaagaaag gaacgtttta caacatacag gtgtggcttg agaagatgac
      361 ctatcgggtc agtgacgtcg ttatggccac caatagcagc tacagagacc tcgccatcac
      421 cagagggggc ttagctccag aagacgtgtt cattgtccgc aacgggcccg atctggggag
      481 tttcaaggct gtccccccaa acgccgccct gaagtacggc aaaccctacc tggtcggcta
      541 cgtaggaacg atgagtgtcc aagagggatt ggatatcctt ttggatgtcg cagcgcacgt
      601 caagaacttg ggacgccgtg atatccattt cacttgtgtc gggggaggtc cgggactggc
      661 cgctctcaag aagatggccc aggacaagaa cctcgaggag atggtcaatt ttacaggcag
      721 agttcctgat cgcgaactgc tggagatctt atcgacagct gacgtctgcg tgaatccgga
      781 caaaccatgc cggatgaatg atatctccac catgattaag atcatggagt atatggctct
      841 cggcaagccc atcgttcaat tcgatttgaa agagggcaga ttcagcgcca aggaagcatc
      901 tttgtatagc gacgacggaa atcaagtaaa tgatttcgca aacaaggtcc tctggttgat
      961 cgagaacccc tatgaacgaa aaaaaatggg cgagttcgga caaagaaggg ttcgggagga
     1021 gctggcgtgg gactattcgg tcgagaatct tctcgctgcc tatcggagag ctttcagcaa
     1081 gaggccatcg tgatcgtcct ggctatccgc cctggggtct tcgacgccag acaggggctg
     1141 cga
//
LOCUS       DATMKH010000519         1025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1206779, whole genome shotgun sequence.
ACCESSION   DATMKH010000519 DATMKH010000000
VERSION     DATMKH010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1206779"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            289..837
                     /gene="rfbC"
                     /locus_tag="VNV63_06190"
     CDS             289..837
                     /gene="rfbC"
                     /locus_tag="VNV63_06190"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015146913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HXC62245.1"
                     /translation="MRIHSTELQGVVVIEPEVFSDARGYFLETYNARKFAEAGIREEF
                     VQDNHSQSKRGVLRGLHYQVEQEQGKLIRVLSGEIFDVVVDLRPGSSTFGKWSGFNLS
                     GTEKKLIWIPKGLAHGFYTLSETAEVVYKVTEFYAPQHERTLLWNDPELAIHWPLRGE
                     PILSEKDKAGHSWKELMKPRRG"
BASE COUNT          257 a          241 c          297 g          230 t
ORIGIN      
        1 cgcgcgaatc agtggaaccg gcggaaaacc gcacaggcgc tgaagatcag ttatagagcg
       61 ctgatctata agattcagga cgccgggctg gtatcccggc gggcctccat acaaagaaac
      121 cagaatgagg gaccgatcgg cctcggcagg aactaatcca cgtttctttt gaattcgctt
      181 ccatttgcgc cctcctctcc acggtttcgc gatattctcc aaagaatagt aaaaagagcg
      241 aaaggcgagt tccggacgtt ttcgtccttg gggcaggtga agagattgat gcgtattcat
      301 tcgacagagc ttcagggagt agtcgtaatc gaaccagagg tattttccga tgcccgcggt
      361 tattttctgg aaacctacaa tgcgaggaaa ttcgcggaag cgggaattcg tgaagaattt
      421 gtacaggaca accattcaca atcaaaaaga ggagtactcc gggggctcca ctatcaggtg
      481 gagcaggagc aggggaaact gatccgggta ttatctggcg aaatctttga cgtcgtcgta
      541 gaccttcggc cgggatcgag cactttcgga aaatggtcgg ggtttaacct gtcggggacc
      601 gagaagaagt tgatctggat tccgaaaggg ctggcgcacg gattctacac gctgtcggag
      661 acggcggagg tcgtctacaa ggttacggag ttttatgctc ctcaacatga gcggacgctt
      721 ttgtggaacg atcccgagtt ggccatccac tggcccttgc gaggcgaacc aattctttcg
      781 gagaaagata aggcggggca ttcctggaag gagcttatga agcccaggcg agggtgaatc
      841 ttaccgaagt cagcttggcg agccgcaacc gctttgcgac cagactgttg ccggaaagat
      901 tgcgcaaagt ccactcagat gggagtggat tgagtgctgg aaagcttata aggaagcatc
      961 caggttttct tcgacgttta ccttaacttc tgccttaact tcccggcccg ctcggatcca
     1021 aatga
//
LOCUS       DATMKH010000520         1314 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3118151, whole genome shotgun sequence.
ACCESSION   DATMKH010000520 DATMKH010000000
VERSION     DATMKH010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3118151"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..420
                     /locus_tag="VNV63_06195"
     CDS             157..420
                     /locus_tag="VNV63_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62246.1"
                     /translation="MTFTDDDQVPKERHIQVFSDLDLKRYKNHDYSLPSQASWNDAID
                     ALLARLEAAELALTDVVVCPCEKGHFHLLKGDHYEAWRKASGK"
     gene            417..743
                     /locus_tag="VNV63_06200"
     CDS             417..743
                     /locus_tag="VNV63_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62247.1"
                     /translation="MSNLDDTQFCPQCEAHAKERDRLQEEVERLKKMPPGIVQQARTC
                     GHTKNNWTCLECYWELQSRAERAEKMARELAEAIRNHFKKCDATAEDSKLEEALAAFE
                     AKEGGK"
     gene            743..934
                     /locus_tag="VNV63_06205"
     CDS             743..934
                     /locus_tag="VNV63_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62248.1"
                     /translation="MQWWWDRHYDCWLLGIGIQRMNNAQHSWSLCLWIGPYVHGIYWG
                     DPVHWVEAVKAYEKEKGGK"
     gene            939..>1314
                     /locus_tag="VNV63_06210"
     CDS             939..>1314
                     /locus_tag="VNV63_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HXC62249.1"
                     /translation="MIFKWDFMERNVAAWAKEYANAPAYPHIALDRLADDNLLSQALA
                     EFPPPGSPVWHQNTVSTATIYKKQAQQDIREIPPTLREVLFEMNSGPFIEFLEKLTGI
                     DHLLPDPHLYGAGIQQTLPGGVL"
BASE COUNT          326 a          314 c          381 g          293 t
ORIGIN      
        1 tgaagccgac cggctcttgg tcccgtcggg atttctgacg ctccagacct atgatgagtt
       61 ccggatgtac gggctcctaa agcgcttggg ctattcgttc ttggggcact ccgtagcggg
      121 gatgcacgtg tggaggaaac gggattggga gagcgcatga ccttcaccga cgacgatcaa
      181 gttccaaagg aacgacacat tcaagtcttt tcagacttgg acttgaagcg gtataagaac
      241 catgattatt cattgccatc acaagcgtca tggaacgatg caatcgatgc tctcctcgcc
      301 cgtctggaag cggcagagtt agctttgaca gatgtggtag tttgtccgtg cgaaaaagga
      361 cattttcatc ttctaaaagg cgaccactat gaagcctggc gcaaagcgag cgggaaatga
      421 gcaatctcga tgacactcag ttttgtcctc aatgtgaagc ccatgctaag gagcgcgacc
      481 ggctccagga ggaagtggag aggctgaaga aaatgcctcc aggaatagtc cagcaagcta
      541 gaacctgcgg ccatacgaag aataactgga catgcctaga atgctattgg gaattgcaat
      601 cccgcgccga acgcgccgag aagatggcgc gggagttggc ggaggccata cgaaatcact
      661 tcaagaaatg tgatgcaaca gcggaagatt ctaagttaga agaagctctc gccgcgttcg
      721 aggcgaagga gggcgggaag tgatgcaatg gtggtgggat cgccactatg attgttggct
      781 cttgggcatc gggattcagc ggatgaataa tgcccaacat tcctggagtc tttgtctatg
      841 gatcggtcct tatgtgcatg ggatttattg gggagatcct gttcattggg ttgaggcggt
      901 aaaggcttat gagaaagaga agggcgggaa gtgaaggtat gatcttcaag tgggacttca
      961 tggagcgcaa tgttgcggca tgggccaagg agtacgccaa cgctccggcc tatccgcata
     1021 tcgcccttga ccgcctcgcg gatgacaacc ttttgagtca agccttagcc gagttccctc
     1081 cacctggtag tcccgtctgg catcaaaata cggtatcaac cgccaccatc tacaagaagc
     1141 aagctcagca ggatatccgt gaaatcccgc cgaccttacg cgaggttctg tttgaaatga
     1201 actctgggcc tttcatcgag ttcctggaga agttgacagg cattgaccac cttctacccg
     1261 acccccatct ttacggcgca gggatacagc agacgcttcc tgggggcgtg ttgg
//
LOCUS       DATMKH010000521         1809 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1525646, whole genome shotgun sequence.
ACCESSION   DATMKH010000521 DATMKH010000000
VERSION     DATMKH010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1525646"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1789)
                     /locus_tag="VNV63_06215"
     CDS             complement(<1..1789)
                     /locus_tag="VNV63_06215"
                     /inference="COORDINATES: protein motif:HMM:NF015655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase adenylyltransferase"
                     /protein_id="HXC62250.1"
                     /translation="MPKTPEQERPSLLGGRGAKRIRQAGSSLQKIVDVFNVRLQRCET
                     FDASRQKEFEHELQTLILLLAEDRVQEARERLNLRLIEHLEKVKAPLTGRLDTVEVRF
                     DNRSSEKWTIVDVRSKDTPAFLYAFSNALSMRGIYVYEVHIKSAGTEVRDRFYISDRQ
                     GRKIEGKKEQEALRMAVVLIKQFTHFLRSAPDPARAMRHFDQLLDKIMEGGNARSSVS
                     LFKTEEGLNLLAQLLGTSDFLWKDFLRMQFESLMPVLEDLKKKKLRPGKNPIRKKLTD
                     LLLHGTTYEEKKKILNEFKDREMFFIDMKHLIEPKAVFTDFSHALTDLAEVVLDEAYR
                     ICHEHLMKRYGPPMLENGKSCPFSICGLGKFGGREMGYASDVEVLFLYGGPGRTRGKN
                     RIENSDYFEHLAQELANFIEARQEGIFHVDLRLRPYGTKGTLANPIERLKAYYSPQGE
                     AAPFERQAFIKLKWVAGDELLGRTMEKHRDRYVYSGEPWNLEEALHLRERQMRELVKP
                     GLVNVKYSAGGIVDVEYEVQYLQIIHGKDHVELRVPSTVEALDQLHRSGIVSKKEHDG
                     LRNGYIFLRALIDAMRIVRGNARDLVLPED"
BASE COUNT          385 a          530 c          437 g          457 t
ORIGIN      
        1 catcctcggg caatacaaga tcgcgggcgt ttccccttac gattcgcatc gcatcaatca
       61 gcgcccttaa aaagatgtac ccgttgcgaa gtccgtcgtg ttccttcttc gagacgattc
      121 cggatcggtg cagttgatcc aacgcctcca cggtggacgg aactctcaat tcgacgtgat
      181 ctttaccatg aataatctgc aggtactgga cctcatattc gacgtccacg atgcctcccg
      241 cgctgtattt cacattcaca agcccgggtt tgaccagctc acgcatctga cgctcccgga
      301 gatgcagcgc ctcttcgaga ttccagggct cgccgctgta gacataacgg tctcgatgct
      361 tctccatcgt tcggccaaga agctcgtcac cggccaccca tttcaacttg ataaatgcct
      421 gccgttcgaa gggagccgcc tccccctgcg gactgtaata ggctttgagc cgttcgatgg
      481 gatttgccaa cgtccctttc gtcccgtagg gtctcaacct gagatcaaca tggaagatgc
      541 cttcctgtct tgcttcaatg aagtttgcca gttcctgcgc caaatgctcg aaatagtcgc
      601 tgttctcgat acgatttttg cctcgggtcc gtcccggacc gccgtagagg aacaacacct
      661 ccacatcaga ggcatatccc atctcgcgcc cgccgaactt ccccagtccg cagatcgaaa
      721 aagggcagga cttcccgttt tccagcatgg gcggaccgta tctcttcatg agatgctcgt
      781 ggcagatccg gtaagcctca tcgagtacga cctcggcaag atccgtcaat gcatgagaaa
      841 agtccgtgaa aacggccttg ggttcgatga ggtgcttcat atcgatgaaa aacatttcgc
      901 ggtctttaaa ctcgttcagg atctttttct tttcttcgta cgtggttcca tgaagcagaa
      961 gatcggtgag ttttttccgt atgggatttt taccgggcct caactttttc ttttttaaat
     1021 cttctaaaac cggcatgagg ctttcgaact gcatccttaa aaaatctttc cacagaaaat
     1081 cgcttgtgcc caggagttgc gccaggagat tgagaccctc ttccgttttg aaaagtgata
     1141 cagaggatct tgcattgcct ccttccataa ttttatccag cagctgatcg aagtgccgca
     1201 tcgcccgggc cgggtccggc gccgatcgca aaaagtgtgt gaactgtttt attaggacca
     1261 ccgccatcct cagagcttcc tgttcttttt tcccctcgat ctttcgtccc tgccggtccg
     1321 aaatataaaa tcggtcgcgg acctccgttc cggcgctttt tatatggacc tcgtaaacat
     1381 agatgccccg catcgaaagc gcattcgaga aggcgtaaag aaaagcgggc gtgtccttgg
     1441 accggacgtc cacgatggtc catttctcgg aactgcgatt gtcgaatcga acctcgaccg
     1501 tgtccaggcg gccggtcaac ggggccttca ccttttcaag atgctcgatc agccgcaggt
     1561 tcaaccgctc cctggcctcc tgaaccctgt cctccgccag gagcagaatc agcgtctgaa
     1621 gctcgtgctc aaactctttc tgtcttgagg catcgaacgt ctcgcagcgc tggaggcgga
     1681 cattgaagac atccacgatc ttctggagag aggagccggc ctgtctgatt ctcttcgcgc
     1741 cccggccccc taagagagac ggccgctcct gctcgggcgt ctttggcaaa aatgtgtaga
     1801 catgtcccg
//
LOCUS       DATMKH010000522         1167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_244208,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000522 DATMKH010000000
VERSION     DATMKH010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244208"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1167
                     /locus_tag="VNV63_06220"
     CDS             <1..>1167
                     /locus_tag="VNV63_06220"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015809599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HXC62251.1"
                     /translation="ILAGDFDGALAVARQQVEGGANLIDINMDEGMIDSDAAMVKFLN
                     LIAAEPDISRVPIMIDSSRWSVQEAGLRCIQGKGIVNSISLKEGEEKFKNSARLVRRY
                     GAAVVVMAFDEQGQADNLARRKEICDRAYRILTEEVGFPPEDIVFDPNILTVATGMEE
                     HNNYAVDFIAAVRWIKENLPFARVSGGVSNISFSFRGNNTVREAMHAAFLYHAINAGL
                     DMGIVNAGQLAVYEEIPKDLLELVEDVLLNRRPDATERLVDFAEKVKSSVKVQVKDEA
                     WRAGPVEERLSHALVKGIVDFIEEDTEEARLKYGKPLAVIEGPLMSGMSIVGDLFGAG
                     KMFLPQVVKSARVMKKSVAFLTPFMEAERAAQVNPKAQGRVLMATVKGDVHDIG"
BASE COUNT          271 a          262 c          381 g          253 t
ORIGIN      
        1 gattttggcc ggagattttg acggcgcatt agccgtggcg cgtcaacagg tggaaggggg
       61 agcgaacctg atcgacatca acatggacga agggatgatc gattcggatg cggcgatggt
      121 gaaattcctc aacttgatcg ctgcggaacc tgacatctca cgggtgccga tcatgatcga
      181 tagttcccgt tggagcgtcc aggaggcggg tctccggtgt attcagggaa aaggaatcgt
      241 caattcaatc agccttaaag agggcgagga aaaattcaaa aacagtgccc gcctcgtccg
      301 ccggtacggc gcggctgtgg tggtgatggc cttcgacgag caaggccagg cggacaatct
      361 tgcgaggaga aaagagatct gtgaccgggc ttatcgaatt ttgacggaag aggttgggtt
      421 tcctcctgag gatattgttt tcgatccaaa catcctgact gttgcgacag gcatggagga
      481 gcacaataac tacgcggtgg acttcatcgc agctgtccgc tggattaagg aaaatctgcc
      541 ctttgcgcgg gtcagcggcg gggtgagcaa tatctcgttc tcctttcgag ggaacaacac
      601 ggtgcgggag gcaatgcatg ctgcgttcct ctaccatgcg attaacgcag ggctggatat
      661 ggggatcgtc aatgccggcc agttagcggt ctacgaggag atcccaaaag atctgctcga
      721 gttggtggag gatgttcttc tcaaccggcg tccggacgcc accgagcgat tggtggactt
      781 tgcggagaaa gtcaaatctt cggtcaaggt tcaggtgaaa gacgaagcct ggcgagctgg
      841 cccagtcgag gagcggttgt cgcacgcact ggtgaaaggg atcgtcgatt ttattgagga
      901 ggatacggaa gaagcccggc ttaagtacgg taaaccgtta gccgtgattg aagggcctct
      961 gatgtccggg atgagtattg tcggggattt gttcggggct ggtaagatgt ttttgcccca
     1021 ggtcgtcaag agcgcacggg tgatgaagaa atctgtggca tttctcactc ctttcatgga
     1081 ggcggaacgg gcagcccagg tgaacccaaa ggcccagggc agagtcctga tggcgaccgt
     1141 caaaggggac gtccatgata tcgggaa
//
LOCUS       DATMKH010000523         3056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4415512, whole genome shotgun sequence.
ACCESSION   DATMKH010000523 DATMKH010000000
VERSION     DATMKH010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4415512"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1125
                     /locus_tag="VNV63_06225"
     CDS             <1..1125
                     /locus_tag="VNV63_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HXC62252.1"
                     /translation="REDVSLKEILSSKVFAEAGTKLALALGKDIFGQPVVADLAAMPH
                     ILVAGATGSGKSVALNTMICSILYASNPQEVKLLMIDPKMLELLTYDGIPHLLCPVIV
                     RPKAAAEILQKMVAEMQQRYRLLAEAGARNIEGYNKALAKSPSGEAKPLPYLVIVIDE
                     LADLMMAVARDVEDSIARLAQMARAAGIHLIVATQRPSVDVITGIIKANFSARISFQV
                     SSKTDSRTILDANGAEQLLGRGDMLYLAPGTGRITRIHGSYISEGEIKKAVDFIKAQE
                     SPTYNPLLLSQATAAGGADSQSSSLEERDELYEKAIDLVLSTGQASASFIQRRMRVGY
                     PRAARMIEIMEEDGILGPSLGGRPREIRVKRSPFQEDRPQ"
     gene            1122..1775
                     /gene="lolA"
                     /locus_tag="VNV63_06230"
     CDS             1122..1775
                     /gene="lolA"
                     /locus_tag="VNV63_06230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HXC62253.1"
                     /translation="MKKTLLFFIGLLIIVTDPLIHSKESAPYTSEIVKKIQNQYQATQ
                     SLEANFVQVNHYEGFTKTFTSKGHLYIKRPGKLRWDYTEPIKQQVFVNENKVWVYVPE
                     HQQAILSPLAQENDSQLPIHLLSWAAQLELEFDVHTDKETETLATLTLIPKEARSGPR
                     KITLEIDPKTALIQKITLYQTDGNTATFTFTQIKINPTLEDPLFVFTPPKGVEVVQP"
     gene            1824..2315
                     /locus_tag="VNV63_06235"
     CDS             1824..2315
                     /locus_tag="VNV63_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007784652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HXC62254.1"
                     /translation="MPDQNSFDIVSQTDLQEVKNAEAQAMKEIRQRFDFKDSKSEIRL
                     EGQDIIVVADDEFKLKNVLDILQSKLVKRGVSLKAITYGTPEQALGGLIRQKLTLQQG
                     IPQEKAKEVVKAIRDTKFKVQSQIQADQIRVSSKSKDELQAVMAFLREKDFGIPLQFT
                     NYR"
     gene            2440..>3056
                     /locus_tag="VNV63_06240"
     CDS             2440..>3056
                     /locus_tag="VNV63_06240"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXC62255.1"
                     /translation="MKRIELRSFMVTLLLGSIVTWSIIFVITIVPVPVFALSANSLFE
                     QFTRADDIQDKKASLEKLKALEPNSAYGHFAKGWLLNLGRRFDEAITEHQEAIRIRPD
                     FAEAHNGLGNAYYNLDKPDEALTEYQVAIFFDPAYIEAYLNIAMLDYQKDRLDQAASA
                     YLTVIQINPKHAIAQNNLGNVYYRQGAVTKAIRAYQISLKINPNLP"
BASE COUNT          799 a          856 c          768 g          633 t
ORIGIN      
        1 cgcgaggacg tatcgctcaa ggaaatctta agctcaaagg tcttcgcaga ggcaggcaca
       61 aagctggcac tggcattggg caaggacatc tttggccaac cggtcgtcgc ggatcttgcc
      121 gcgatgccgc acatcctcgt cgcaggagcc accggctccg gaaagagcgt ggcgcttaac
      181 accatgatct gcagtatcct ctatgcaagc aatccccaag aagtcaaact cctgatgatt
      241 gatcccaaga tgctcgagct cctgacctat gacggaatac cccacctcct ctgtccggtg
      301 atcgttcgac ccaaagccgc ggccgagatc ctccagaaga tggtcgccga aatgcagcag
      361 cgctaccgtc tcctggccga ggcgggtgct cgaaatattg aaggatacaa caaagccctg
      421 gcaaaatccc catccggcga agccaagcct ctcccctatc ttgtgatcgt aattgacgaa
      481 ctggcggacc tcatgatggc cgtggcccgc gatgtggagg attcgatcgc tcgcctggcg
      541 cagatggccc gtgccgccgg cattcatctc atcgtcgcca ctcagaggcc atccgtagac
      601 gtcatcaccg gaattatcaa ggccaacttc tcggcgcgga tctcattcca ggtctcatcc
      661 aagaccgact ctcgtaccat tctcgacgcc aacggtgccg agcagctgct gggccgcggt
      721 gacatgctgt atcttgcacc gggaaccggc cggatcacgc gtatacacgg ctcctatatc
      781 tcggagggcg agatcaaaaa agcggtagat tttatcaagg ctcaagaaag tcctacgtat
      841 aatccgctcc tcttatccca agcaaccgcc gcaggcggcg cagattcgca gtcgtcctct
      901 ctcgaagagc gggatgagtt gtatgagaag gcaattgatc tggtgctttc caccggccag
      961 gcctcagcct catttatcca acgccgcatg cgagtgggtt acccgcgggc agcccgcatg
     1021 atcgagatca tggaggaaga cgggatactg ggaccgtctc tcggcgggcg gccccgagag
     1081 atccgggtca agcgatcgcc ctttcaggag gaccggcccc aatgaaaaaa acacttctct
     1141 ttttcatcgg tctccttatc attgtcaccg atccattgat tcatagcaag gaatccgcac
     1201 catacacctc agagatcgtt aagaagattc agaatcaata ccaggccact cagagtctgg
     1261 aggcgaactt tgtacaggtg aatcattatg aaggcttcac caaaaccttt acctccaaag
     1321 gccaccttta tatcaagcgg ccgggcaagt tgcgctggga ttatacagag ccgatcaaac
     1381 agcaggtctt tgtgaatgag aacaaggtct gggtatacgt gccggaacat cagcaggcaa
     1441 tcttaagccc cctggcgcag gagaatgatt cgcaactgcc gatccacctc ctgtcctggg
     1501 cagcacaact cgaactggag tttgacgtcc ataccgataa agagacggaa acccttgcga
     1561 ccttgacctt aatccccaaa gaagccagga gcggccccag aaaaatcacg ctcgaaattg
     1621 atcccaagac cgctttgatc cagaagatca cgctgtacca gacggatgga aacacggcta
     1681 catttacttt cacgcagatc aagataaacc cgacgctgga ggacccttta tttgtgttca
     1741 caccacccaa aggcgtggag gtggtgcagc cttagtcggt aatccattaa tgggcaaaca
     1801 atcagcaatt cggaggtaag accatgccgg accaaaactc ttttgatatc gtttcacaga
     1861 ccgacctaca agaggtcaaa aatgcagagg ctcaagcgat gaaggaaatc cgccagcgtt
     1921 ttgatttcaa agacagcaaa agcgagatca ggcttgaagg ccaggacatt atcgtagtgg
     1981 cggacgatga gttcaagtta aaaaacgtcc tggatatact ccagtccaag ctggttaaac
     2041 gtggtgtgtc cttgaaagcg atcacctacg gcacgccgga acaggcattg ggaggtctga
     2101 tccgtcaaaa gctcacactc cagcagggca tcccccagga aaaggccaag gaagtcgtga
     2161 aggcaatccg ggatacaaag ttcaaggtcc aatcccagat ccaagcagac cagattcggg
     2221 tttcaagcaa aagcaaggat gaactccagg ccgtgatggc ctttctccgc gaaaaagatt
     2281 tcggcattcc tctccagttc accaactacc gctagggaat tgaagaaccc cgccccagag
     2341 gggcgaggtt tgcttcagaa ttgcgctcgc gatgcgcgtt caaacggctt gacagttaca
     2401 gtctttctgc taccctttaa ataagagggt agatctgtca tgaaacgtat tgaactgcga
     2461 tccttcatgg tcacgcttct tctcggcagc atcgtaacgt ggagcataat ctttgtaatc
     2521 accatcgtgc ccgtgccggt ctttgcgctt tcagcaaaca gcctcttcga acaattcacc
     2581 agagcggatg acatccagga caaaaaggcc agcttagaaa aactcaaggc gttggagccc
     2641 aactctgctt acggccattt cgcaaaaggc tggctgctca atctcggacg gcggtttgac
     2701 gaggcgatca cggaacacca ggaggccatc cggatcagac ccgactttgc cgaggcgcac
     2761 aatggtttgg gcaatgctta ctataacctg gacaagccgg acgaggccct gacggagtat
     2821 caggttgcta tttttttcga tccggcctac atcgaggcct accttaatat cgcaatgctc
     2881 gactatcaaa aggaccgtct ggatcaggcc gcatccgctt atctgacggt gatccagatc
     2941 aacccaaaac atgccatcgc tcagaacaac ctgggcaatg tctattatcg gcagggagcg
     3001 gttacgaagg cgattcgggc ttatcaaata tcgttaaaga ttaatcccaa cctccc
//
LOCUS       DATMKH010000524         1563 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_886717,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000524 DATMKH010000000
VERSION     DATMKH010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_886717"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..952
                     /locus_tag="VNV63_06245"
     CDS             <1..952
                     /locus_tag="VNV63_06245"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXC62256.1"
                     /translation="FWLADGGGRLIYVNSAFYRQFGAEGGFAGRSLQEMLPRDMAVFF
                     SEKHRRVSQSGVAYRKVHHFTRLDGRCCSFLVHIYTIDGSGASLTGGEAWDVSDFADE
                     RSEQEQKFAETIVQVQEQERTYIGHELHDNVIQIMISARLHLEGLKGRTKEEKAVKAK
                     TTDLILAAIEETRRLSRKLAASQLKNAGLLTAIAQLLNDLRDIGRFNIKFNSSLDIDA
                     EYCLREEKKVAIYRIVQEQINNIIRHSKAKNIRVNIMQNNSGVILYTFDDGIGFDLQK
                     VPKGMGLAGIQSRAKAFKGMVDIVTAPGKGCSITILIPYD"
     gene            958..1311
                     /locus_tag="VNV63_06250"
     CDS             958..1311
                     /locus_tag="VNV63_06250"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXC62257.1"
                     /translation="MKRILIIDEDFDWLAAMRSVFERSGYETSLASSYPEGYDLLEAI
                     LPDLVFLDITPGDPNGLLLYKKIKQQEEFIHIPIVVTSHNADATKLYRPYGESAFLKK
                     PFQLSRLMKTLRIYL"
BASE COUNT          376 a          399 c          433 g          355 t
ORIGIN      
        1 attctggttg gcagatggcg gcggcaggct tatttacgtc aacagcgctt tttaccggca
       61 atttggtgca gaagggggtt ttgccggtag gagcctgcag gaaatgctcc cgagggatat
      121 ggccgttttt ttttctgaaa aacatcgccg ggtgagtcag tccggcgtcg cctaccggaa
      181 ggtgcatcat tttacccgac tggatggaag atgctgcagc tttctggttc atatctatac
      241 catcgatgga agcggtgctt cgctaacagg cggagaagcc tgggatgtaa gcgattttgc
      301 ggatgagcgg tccgagcagg agcagaagtt cgccgaaacg atcgtgcaag tccaggagca
      361 ggagcggacc tatatcggcc atgagctgca cgacaacgtc atccagatca tgatctcagc
      421 ccggctccac ctcgaaggcc tgaaaggcag aacgaaggaa gagaaagcag tgaaggcaaa
      481 gacaaccgat cttatcctgg ctgccattga agagacgcgc cgcctgtcga ggaagctggc
      541 agcttcccaa ttgaaaaatg ccggcctgct tacagcgatc gcgcagctgc tgaatgatct
      601 gcgggacatc ggccggttca acatcaaatt caattcttcg ctcgatatcg atgccgaata
      661 ttgtctgcgt gaggagaaga aagtggcgat ataccgcatc gtgcaggagc agatcaataa
      721 tattatccgg catagcaagg caaagaatat ccgggtcaat atcatgcaga acaattcagg
      781 ggtcattctc tatacgttcg atgacgggat cgggtttgac ctgcagaaag tcccaaaggg
      841 gatgggtctt gccggtattc aatcccgggc taaggccttc aagggaatgg tggatatcgt
      901 tacggctccc ggtaaagggt gttcgatcac catcctgatc ccctatgact gagcagaatg
      961 aagaggatac tgatcataga tgaagacttc gactggctgg cagccatgcg gtcggtcttt
     1021 gaacgaagcg gatacgaaac ttcgcttgcc tcctcttatc cggaaggcta cgatctgttg
     1081 gaagcgatcc tgccggacct tgtctttctg gatattacgc ccggtgaccc gaatggtctc
     1141 ctactctata agaaaatcaa acaacaggaa gaatttattc atatccctat cgtcgtgacc
     1201 tcgcataacg cggacgccac aaagctttac cggccctatg gggagtcggc cttcctgaaa
     1261 aagccctttc agctttcgag gctgatgaaa acattgcgga tctatttgta aggatatcag
     1321 ggcccgtcgg aaggcccccc gcccgagctt cgctcgggcg gacaatagtg gtcgttgatc
     1381 gttctttcct ctgcaaccgc gtacctcaga aatcggattc gtcctatgca gaccgtctcc
     1441 atttctaccc tcctcccggc ctttcgtcca ggatacggcg gggggaggcg tagggtggtc
     1501 ctacactatt cagcgcgcaa actattcacc ggattggcca gggccgccct ggctgcccga
     1561 aca
//
LOCUS       DATMKH010000525         2012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_246726,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000525 DATMKH010000000
VERSION     DATMKH010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246726"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /locus_tag="VNV63_06255"
     CDS             complement(<1..797)
                     /locus_tag="VNV63_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016877523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HXC62258.1"
                     /translation="MADQKGTTQKSSCHPLGATPDAEGVNFSIFSYDAKGVELLLFDK
                     PKDLEPTHVIPLDPAVNTTFFFWHIYVSGLRPGAHYAYRVDGPREDSQRKEGQGSLAL
                     GHRFNKNKVLIDPYAKGNTNTFWDRVAACGPDDNVKTSMRSIVIDAASYDWEGDMPIH
                     RPMRDTVIYELHVGSFTKSPTSGVTHPGTFSGLIEKIPYLKGLGITAVELMPVMDFDD
                     KEVMHLSPTDGRPLTNFWGYSSHSFFAPKCAYCVSYEEGSHLRDFRD"
     gene            1109..1714
                     /locus_tag="VNV63_06260"
     CDS             1109..1714
                     /locus_tag="VNV63_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62259.1"
                     /translation="MKRKVAAVHTLKQARAYILQVGICGIFSDAGVGMPNLWEVVDLP
                     DRQPGEKGWGQKIIAIWRWKNELPAIYPAEIFYGKIPAGRAVLMSMDYLRIEHYSKHH
                     RPLRECSSLAQKIYERLKLDPLTTGSLREELNMTQRPERNRFERALQELQITLNIARR
                     NSLEDENDTWVLFSDQYLDVVHAGELSHRDHELTSDRHRSL"
     gene            1711..1890
                     /locus_tag="VNV63_06265"
     CDS             1711..1890
                     /locus_tag="VNV63_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62260.1"
                     /translation="MNAQEAQTLRLQEIVVWTPDGSKGEVIAIDGSGVKVRWEDDQCG
                     YYQFTDLLSQIERLR"
BASE COUNT          497 a          513 c          526 g          476 t
ORIGIN      
        1 atgtctctga agtcacgaag gtgactgccc tcttcgtacg aaacacagta agcgcactta
       61 ggagcaaaga agctgtgtga gctatagccc caaaagttcg ttaggggtct tccatccgtc
      121 gggctgaggt gcatcacttc cttgtcgtcg aagtccatta ccggcatgag ttctacggcg
      181 gttatgccca accctttcag gtaaggaatc ttttcgatga ggccggaaaa ggttcccgga
      241 tgggtcactc cacttgtggg cgacttcgta aagcttccga catgcagctc gtaaatgacg
      301 gtatcgcgca tcgggcgatg gattggcata tcgccttccc aatcgtaaga tgccgcatct
      361 atcacgatgc tgcgcatgga agttttcacg ttgtcatccg gcccgcaagc agcgacgcgg
      421 tcccaaaagg tattcgtgtt tcctttggcg taggggtcga tgagcacctt gtttttgttg
      481 aagcggtggc ccaatgccaa tgaaccttga ccctcctttc tttggctatc ctctcttggc
      541 ccatccactc ggtaagcgta gtgcgcgccg ggtcgcaggc cgctcacata gatgtgccag
      601 aagaagaacg tcgtgttcac ggccggatct agcgggatta cgtgggtggg ctctaaatcc
      661 ttgggcttat caaaaagcaa cagttctact cccttggcgt cataggagaa gattgaaaaa
      721 ttgacgcctt cagcgtccgg ggtggcaccc agcggatgac acgagctctt ttgggtcgta
      781 cccttttggt cagccatatc tcttttctct ctacttcaag ccgagatccg acaacaaaga
      841 gacaaacgga gaattctggc gaagccggtc ggcgcgttca gccgaacttt ccagtaatct
      901 aatcagttgt aaaggggacc acacgaggtt ctgaatgagg acaggtaaac tgttggcctt
      961 ccaaaggttg tcgctatgca gcaagaccag tactgacgtg ttccctttga cctttctttc
     1021 aaccccgaac tcgattgcct atctcagcat cgttccctcg cccgttttct attatggaga
     1081 tctttatcgg cgagctatga tacactcgat gaaacgcaag gtagctgccg tccatacctt
     1141 gaagcaggct agggcctata tcttacaagt cgggatatgc ggcattttct ccgacgccgg
     1201 agttggtatg ccgaacctat gggaggtggt cgatttacct gacaggcaac caggagaaaa
     1261 aggctggggc caaaaaatca ttgcgatctg gcgttggaaa aatgaactac cggctatcta
     1321 tcccgcagag atcttctacg gaaagattcc ggctggtcgg gccgtattga tgagtatgga
     1381 ttaccttcgt atcgaacact attcgaaaca tcatcggcca ctgagggaat gcagctcctt
     1441 agcacagaaa atctatgaaa ggctgaagct ggatcctctg acgaccggtt cgttgagaga
     1501 agaattgaac atgacccagc gtccagagcg gaaccgattc gagcgggcgc tacaggaact
     1561 ccagatcacg ttaaacatcg cccgccgcaa tagtctggaa gatgagaacg atacctgggt
     1621 tctctttagt gatcagtatt tagacgtggt gcatgcaggg gaactgtcac atcgggatca
     1681 cgagctcact tcggaccggc atcgctcctt atgaacgccc aagaagctca gactctccga
     1741 ctccaagaga ttgtcgtctg gaccccggat ggatcgaagg gcgaagtgat tgcgatagac
     1801 ggttccgggg tcaaagtccg ctgggaagac gatcaatgtg gctactatca atttaccgac
     1861 ctattatctc agatcgaaag gttacgttga gtgcagtaca agagcccatc ccaagaagtc
     1921 tctaagctcg gattattttg aacagaagac cgcaaagcgg gtgacccgca accaaagcag
     1981 agaaaatctc tcgcaaagca gacttcgccc ca
//
LOCUS       DATMKH010000526         1363 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_564771,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000526 DATMKH010000000
VERSION     DATMKH010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1363
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564771"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="VNV63_06270"
     CDS             <1..284
                     /locus_tag="VNV63_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007142567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXC62261.1"
                     /translation="HISAKRSGKDWIFSISDNGIGIDPKHFEQIFEIFKRLHNQKDKP
                     GTGIGLAVCRRVVNRHGGKIWVESEPGHGSTFHFTIPEGTEQTNEQSTR"
     gene            265..750
                     /locus_tag="VNV63_06275"
     CDS             265..750
                     /locus_tag="VNV63_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016869399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXC62262.1"
                     /translation="MSNQLANGRPAEILLVEDNDDDVELTRQGFRRAKLLVNLHRVED
                     GEKCLAFLRKQGEYQNAPTPDLILLDLNMPRMSGREVLAEMIKDDHLKGLPVVILTTS
                     EQEEEVLKMYKMRCSSYIVKPVDFDQFLKVVRAIAEYWFTVVVLPNVGSTEKATAPAG
                     V"
BASE COUNT          330 a          337 c          376 g          320 t
ORIGIN      
        1 tccacatctc agccaaacgt agcgggaagg actggatctt ttccatcagc gacaacggta
       61 ttggcattga ccctaagcat ttcgagcaga tctttgaaat attcaaacgg ttgcacaatc
      121 aaaaggacaa accgggaaca ggtattggat tagcggtctg ccgtcgtgta gtaaatcgcc
      181 acggtgggaa gatttgggtg gaatccgaac ccggccatgg aagcacattt catttcacga
      241 ttcccgaagg gacggaacaa accaatgagc aatcaactcg ctaacggtcg gcctgctgag
      301 atcctgcttg tcgaagacaa cgacgacgat gtagagctta cccgccaagg gtttcgacgg
      361 gctaagctgc tcgtcaactt gcaccgagtg gaagatggtg agaaatgttt ggccttcttg
      421 cgcaagcagg gtgaatacca gaatgctccc acgcctgatc tcatacttct cgatttgaat
      481 atgcccagaa tgagcgggcg ggaagtactc gctgagatga tcaaggatga tcatctaaaa
      541 ggtctgccag tcgttatctt gaccacttcg gagcaggaag aagaggtctt gaaaatgtac
      601 aagatgcgct gtagttctta cattgtcaaa cctgtcgatt tcgatcagtt ccttaaggtc
      661 gttcgggcaa tcgctgaata ttggtttacg gttgtggttt tgccgaacgt tggttcgacc
      721 gaaaaagcga cggcacctgc gggggtctga ggagcccgga aaaaactcgg cctcggggga
      781 taatcctgtc cgctggaacc cggcattctg cgaagccgac ccaccttcta gctgtgcgtc
      841 tggttgcgga agtacctttg agtgatctat taacagtcat ggtggcggat cgctctggcc
      901 ctgggatgtg ggccagtgga tgcccatgca agtgcggggg acgcgataca caggaagttt
      961 gaccgggagc acctcaccga ccggaggtcc cgacagtcgg atcaacggtc tgagcacacc
     1021 tgcttgctgg tccataaggc ccacagcgct tatggagcag taggtgcagg ggagaagacc
     1081 catgttcagc tctccatctc gcaaagggtg atgattgggc tacacccatt tctctggatc
     1141 gcggttgggg agtgtctttt ctaaggcctc taagggccga taagcccgca tactcacaga
     1201 tgcctccatg attcgatcct cacgatctta cgaagtgatg atcgcgagac atcaaaacgg
     1261 tcggcgagat ccttctgttg aaacttgcca gttgcgtaca gcttccgaat ctgaagcact
     1321 tgttttgcat tcaacttggc attcggatgc tcttccccca tga
//
LOCUS       DATMKH010000527         1192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4082848, whole genome shotgun sequence.
ACCESSION   DATMKH010000527 DATMKH010000000
VERSION     DATMKH010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4082848"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            217..609
                     /locus_tag="VNV63_06280"
     CDS             217..609
                     /locus_tag="VNV63_06280"
                     /inference="COORDINATES: protein motif:HMM:NF019149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HXC62263.1"
                     /translation="MSKTGNASGDNAFSRGALYSMLQNRIYLGEITHKDKSFPGQHPA
                     IIDQNLWDRVQVQFKANLQAERKRPRATSTSLFTGLLYDEVGNRFTPSHATKKSRRYR
                     YYVSQALTKVDLKSQQTRVVFLPKRLRN"
     gene            764..>1192
                     /locus_tag="VNV63_06285"
     CDS             764..>1192
                     /locus_tag="VNV63_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HXC62264.1"
                     /translation="MVTRIIVRHQKIELQLSKHAVLKTFLTPQRSEEIAGTLTASEDL
                     IILKADAKLRRCGGEVRLLLAEAGQNRARPVLSLVRAVARAHDWMDRILRGEIPNQRA
                     LAKATGFDERYISRIIPLAFLAPDIIEAILEGKQAPDLSLT"
BASE COUNT          306 a          329 c          304 g          253 t
ORIGIN      
        1 gaacgtatcc gcgacaagat cgccgcgtcg aagcgcaaag gcatgtggat gggaggagtc
       61 gttcctctgg gctatgacct cgaagatcgt cacctggttg tcaatccata agaggccaat
      121 cgggtcaagg atatctttca agcctatctc gctgaaggat gcgtctccag gctttagatt
      181 tatctggaga agaaggacgt ccgaagaaaa aagagattga gcaagaccgg aaatgcctcc
      241 ggtgacaacg ccttttcccg tggagcactc tattccatgc tccagaaccg aatctacctc
      301 ggcgagatca cgcacaagga caagtccttt cccggccagc atcctgccat catcgaccag
      361 aatctctggg atcgtgttca agtgcagttc aaagccaatc ttcaggctga gaggaaacgg
      421 ccgcgagcaa ccagcacaag ccttttcacg ggtctgcttt acgacgaggt tgggaatcga
      481 tttactccgt ctcacgctac gaagaagagc cgacgctacc gatactatgt ctcccaggct
      541 ctaaccaagg tagatctaaa aagccaacag accagggtcg tcttcctgcc gaagaggttg
      601 aggaattgat cttgactcaa ctcgccgccc ttttgcagtc agcaccgaga atcctcgatc
      661 tcttgcaacc ccactccttc gatatgacag agactcagca tgttgcgaaa gtcgctcggg
      721 aatatttcgc ttctcaggaa aagtccggaa tgacctgcga tcgatggtta cgcggattat
      781 cgtgcgacat cagaagattg agctgcaatt gagtaaacac gccgtcctga aaaccttcct
      841 cacgcctcaa cgaagcgaag agatcgccgg cactctaact gcttcggaag atctcatcat
      901 cctcaaagcg gatgccaaac tacggaggtg cggaggcgaa gttcgcctct tactggccga
      961 ggccgggcaa aacagagcgc gccctgtact ctctttggtt cgagcggtcg cacgagcaca
     1021 tgactggatg gatcggattc tgcgcgggga aatacccaat cagcgcgctt tggcaaaagc
     1081 gaccggcttc gatgaacgct acataagcag gattatccct ttggcctttt tagctcccga
     1141 catcatagaa gcaatccttg agggaaagca ggcccctgac ctctcgctga cg
//
LOCUS       DATMKH010000528         5699 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_889416,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000528 DATMKH010000000
VERSION     DATMKH010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 5699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5699
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_889416"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VNV63_06290"
     CDS             <1..228
                     /locus_tag="VNV63_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62265.1"
                     /translation="VPMNPASPSLFLAVKEGDVTRGLICVETGEIGIKVINHLEQLSP
                     STKSDIFSFENLESFRWEPLFPIQAPAVSAP"
     gene            483..1526
                     /locus_tag="VNV63_06295"
     CDS             483..1526
                     /locus_tag="VNV63_06295"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HXC62266.1"
                     /translation="MSDRWSVLKKLLKRVSRSFYLSLIILPSETRQQVSLAYLFCRAA
                     DTIADTDLLPASSRLTYLSSLQGQFRSSPSFSAIGKIKESFVPLQTHKAERALLEHLE
                     ECFQAYLLFSPEDQELIRWLVLTLTHSMEMDLNCFPSDGSAPQALPAFSDLDLYCYYV
                     AGCVGEFWTKAHRLHLSSLQNWDEKSMCEKGIRFGKGLQMTNILRDLPRDLRLGRCYI
                     PETMLKEQGLSAMELLSPQALPRLRPLLHSLIRQTLEHLDRAWEYTMEIPRQEPRLRL
                     ACLLPILLALKTLKLVASSDQLLDPSVSVKISRLDVYKTVGLALCLVRSNSLLTHIFR
                     RLRNSISITVSAL"
     gene            complement(1515..2843)
                     /gene="rlmD"
                     /locus_tag="VNV63_06300"
     CDS             complement(1515..2843)
                     /gene="rlmD"
                     /locus_tag="VNV63_06300"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HXC62267.1"
                     /translation="MQLRPGNRLTVTIEKLVHGGHGLARQEGRVLFIPEVLPGETVQV
                     EVQKIQKDVAFARLLEVTHPSPHRISPPCKIYSECGGCQIQHIEDSNQLEIKNALIRE
                     ALHRIGRITEINLQPIIPPPSAYGYRTRTQLKVRYTDQQVRLGFYREGSHHHVPVEEC
                     LVLHPELNKILKPLEQILNEERFRAIGLTSVHLNLSAATREVLIRLLLDTRLHALHDL
                     KETGETLYNRFREAIGSLAGLVLSPRDEPRLVFGRDFVEERFGEYRYRISEGSFSQIY
                     PEAADLLIQQAIRLADLAGHERVLELHCGIGTISLPVAARSIHLRGLDANRIAIEDAQ
                     FNAEANQVKQIEFLCTDAEQGLRQCLAEGQKYDRLILDPPREGLSKTAVDLASQLVPS
                     KIIYVSCEMATLARDLQRFCEQGYRLGQLQPVDMFPQTAHVELIAELLQG"
     gene            complement(3056..4432)
                     /locus_tag="VNV63_06305"
     CDS             complement(3056..4432)
                     /locus_tag="VNV63_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXC62268.1"
                     /translation="MSDTILVVDDEPSILRTLSGVLKDAGFSVLIAQDGPEALRLLRQ
                     DIPRLILLDIWMPEQDGLETLKKIKELYPQMLVVMMSGHGSIETAVKATKLGAYDYIE
                     KPLSLEKVTLVIKHALHEQRLEEENFRLRARISRQQTLVGESLPIKRLQEIIRTAGPT
                     QSRVLISGENGTGKELVAHSIHQHSLRADQPFVEVNCAAIPETLIESELFGYEKGAFT
                     GAMAMKRGRFELAHGGTLFLDEIGDMSLATQAKVLRVLQEQHFQRVGGTKLIEADVRV
                     IAASNKNLQEEIKKADFREDLFYRLNVIPIHVPPLRDRKEDIPLLIQHFIAFTAEEQG
                     LKPKQITKEAAELLTHYDWPGNVRELKNMVERLMILVPHPVITPEDLELLFEGQLPAE
                     LMSAPKTSDHLPLRQARAVFEKEFISQRLKENGWNISKTAEDLQIERSHLHRKIKLLG
                     IEIKPENG"
     gene            complement(4429..>5699)
                     /locus_tag="VNV63_06310"
     CDS             complement(4429..>5699)
                     /locus_tag="VNV63_06310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXC62269.1"
                     /translation="FGFYLAKSITVPITKLAEGTQAVAQGNLDFRIKVKANDEIGMLV
                     DSFNKMTADLKTGKTQLEEANLSLSQSNIEINRRRAYMETILEQIATGVIALDAQGRI
                     TNCNRSAEQILGLTAEEILDKSARDAFQSRQLDRLMTLLDKFKRSGKDDLKEEIHLEV
                     MKKPLTLRFNLSALRDPEGRSLGVVMVFEDLSELLKAQKVAAWQEVAQRIAHEIKNPL
                     TPIQLSTERLRKKFFEHSPDFDGIFDEATGTIINEVNSLKTLVDEFSNFARFPSPRPS
                     PQNLHEILNEVIRLYKTAHRDVEFETRYAEDLPTLMLDREQWKRAFVNIFENAIEAMN
                     GGGSLIISTLYDPKRHRIRLEIADNGAGIDPEDFDKLFLPYFSRKKSGTGLGLAIVNR
                     IVNDHNGQIRITLNVPKGATVIIELPVTQT"
BASE COUNT         1198 a         1533 c         1515 g         1453 t
ORIGIN      
        1 gtcccgatga acccggcctc gccatcgctc ttcctggcgg tgaaggaagg ggatgtgaca
       61 cgcggcctga tctgcgttga gacgggagag ataggcatca aagtgatcaa ccatctcgag
      121 caactcagtc cctccaccaa gagcgacatc ttttcgtttg aaaaccttga atcgttccgc
      181 tgggagcctc tattccccat acaggcccca gccgtctcag ctccgtagaa gttgaagaac
      241 cccaccccag aggggcgagg attcttcgat ccgtatggaa gggatcatat ttaattcgct
      301 cgctaacctt tccctaaagg ccgaggattg cgctcgcgat gcgtgttcaa cggtttttcc
      361 ccttgacaga gagacgaaag aaagctaacc tgatttccag tattaagtaa acgatggtac
      421 tcttaggcgg tattcttaaa tgacgagaga taccgcttgg taacggagcc gcgagtcaga
      481 gagtgagcga ccggtggtca gttttaaaaa agcttctgaa acgggtctcc cgctcttttt
      541 atcttagcct gatcatcctt ccttcggaga cccgtcagca ggtctccctc gcttatcttt
      601 tctgccgagc cgctgatact attgccgaca cggatcttct ccccgcatct tcgcgtttga
      661 cttatctttc gtctctacaa ggtcagttcc gttcctctcc gtcattttcc gcaattggga
      721 agatcaagga gagttttgtc ccgcttcaaa cccacaaggc ggaacgagct cttctagaac
      781 atcttgagga atgtttccag gcgtatcttc tcttctctcc ggaggatcag gagctgatcc
      841 ggtggctggt tctgacgctg acccactcca tggagatgga tctcaattgt tttccctcgg
      901 atgggtcggc tccgcaagcc ctccccgcat tttctgatct ggatctctac tgctactatg
      961 tggccgggtg tgtaggagag ttttggacaa aagcccatcg tcttcatctt tcgtcgttac
     1021 agaactggga tgagaaaagt atgtgcgaaa aggggatccg gtttggaaaa ggacttcaga
     1081 tgacgaacat actgcgggat ctgcctcggg atttaagact cggccggtgc tacattccgg
     1141 aaacgatgct gaaggagcaa ggactttccg ccatggagct tctctctcca caagctcttc
     1201 ctcgtctgag gccgctcctt cactctctca tccgccagac actggaacat cttgaccgcg
     1261 cctgggaata cacgatggaa attccccgtc aagaaccgcg cttacgcctt gcctgccttt
     1321 tgccgatcct gttagcgctc aaaaccctga aacttgttgc atcgtccgat cagcttcttg
     1381 atccatctgt ttctgtgaaa atctcccgtt tggatgtgta caagacagtc ggcttggccc
     1441 tttgccttgt ccggtctaac tctcttttga cgcacatctt tagaagactt cggaactcca
     1501 tctccataac cgtctcagcc ttgtagaagt tcggcaatca attccacatg tgcggtctgc
     1561 ggaaacatat ccacgggctg gagttggccc agacgatagc cctgttcgca aaagcgttga
     1621 agatctctgg ccagagtggc catctcacac gagacataaa taatctttga agggaccagt
     1681 tgagaggcga gatcaaccgc ggtcttactc aatccttcgc gcggtggatc taaaatgagt
     1741 cggtcgtact tctgaccttc ggccagacat tgtcggagac cttgttccgc atcggtgcaa
     1801 aggaactcaa tttgcttcac ctgattagcc tccgcgttga attgcgcatc ctcaattgcg
     1861 attcgattgg catctagccc ccggagatgg atggaacgag cggcgaccgg caggcttatt
     1921 gtcccgatcc cgcagtgaag ctctaaaacc cgctcatgac ctgcgaggtc cgccaggcgg
     1981 atcgcctgct ggattaagag atcggcggcc tcgggataga tctgtgagaa agagccttcg
     2041 ctgatccgat aacgatattc tccaaagcgt tcctcaacga aatctctacc gaacacaagc
     2101 cgtggttcat cgcgcggtga gagaacgagt cccgccagcg agccgatcgc ctctctgaac
     2161 cggttgtata aggtctcgcc cgtctctttg agatcgtgca atgcatgcag acgggtatcg
     2221 agcaagagac ggatcaagac ttctcgggtg gcggctgata gattgagatg gacagaggtt
     2281 aatccaatgg cccgaaagcg ctcttcattc agtatctgct ccaagggttt tagaatttta
     2341 ttcagctccg ggtgaagcac gaggcactcc tcgaccggga catgatgatg actgccctcc
     2401 cgatagaagc caagccggac ctgttgatcc gtataacgga ccttgagttg ggttcgtgta
     2461 cggtagccgt aagcggaggg cggagggatt atcggctgca ggttaatttc ggtaatccgc
     2521 cctatccgat ggagcgcttc acggatcagg gcgtttttaa tctccagttg attcgaatct
     2581 tctatatgct ggatctggca tccgccgcat tctgagtaga tcttgcaggg tggagaaatt
     2641 cggtggggtg acggatgggt gacttctaac agtcgggcga aggcgacgtc tttctggatc
     2701 ttttggacct ccacctgtac agtctcgccg ggcaagacct cggggataaa gagcacacgg
     2761 ccttcctgcc ttgcaaggcc gtgtccgccg tgaacgagct tttcaatcgt gaccgtgaga
     2821 cggttgccgg ggcggagttg catagaattg attaacttat ttggttgagg tctgcaattg
     2881 ttcctttcgc tcactcacca aatttttgac ccaactatgg ctcggtcgct cgaatcgtca
     2941 aactcggcct tcagcctcgg acagtgccta ttcgcccttt cagggacgct tccttcgcca
     3001 agatgggtgc ccaaaaattt agtaacgttc gctcaagaaa caattgcagc atggtttagc
     3061 cgttttccgg tttgatctcg attcccagga gtttgatctt ccggtgaagg tggctccgct
     3121 caatctggag gtcttccgca gttttggata tgttccagcc gttttccttg agccgttgcg
     3181 agataaactc cttttcaaag acggcccggg cctggcgaag cgggaggtgg tctgaggtct
     3241 tgggagcgct catcagttcg gccggaagct ggccttcaaa gagcagttcc aggtcttcag
     3301 gcgtgatcac ggggtgcgga accagaatca tcaatcgctc caccatgttt ttcagctctc
     3361 gaacatttcc gggccagtca tagtgagtta ataactccgc ggcctctttc gtgatctgct
     3421 tgggtttcag cccctgttcc tccgccgtga aggcgatgaa atgctgaatg aggagaggaa
     3481 tgtcttcttt acggtcgcgc agcggcggga catggatcgg gatcacgttg agacggtaaa
     3541 agaggtcttc ccggaagtcc gcttttttga tctcctcctg aaggttttta ttcgaggcag
     3601 caatcacccg gacatccgcc tcgatcagct tggtcccgcc gacccgctgg aaatgctgct
     3661 cctgaagcac ccgcaagacc tttgcctggg tggcaaggct catgtcgccg atctcgtcta
     3721 agaacagggt tccgccatgg gcgagttcga aacggccgcg cttcatggcc atggccccgg
     3781 tgaaggcgcc cttctcatag ccgaacagct cgctctcgat caaggtctcc gggatcgcgg
     3841 cacaattcac ctcgacaaag ggctgatccg cgcgaaggct gtgttggtgg atggaatgcg
     3901 ctacgagctc cttgccggtt ccgttttcac ctgaaatcag gacccgactc tgagtcgggc
     3961 cggcggtccg gatgatctcc tggagacgct ttatggggag actttctccc accagggttt
     4021 gttgccggct gatcctggcc cggaggcgga agttttcctc ttccagtcgc tgctcatgga
     4081 gggcatgctt gatgaccagt gtgacttttt caagcgacag cggtttttcg atatagtcat
     4141 aagcgccgag cttcgttgcc ttgacagcgg tttcaatcga gccatgaccg gacatcatca
     4201 cgaccagcat ctggggatag agctccttga tcttctttaa ggtctccagg ccgtcttgtt
     4261 ccggcatcca gatatcgagc agtatcaggc gcggtatatc ctggcggaga agccggagcg
     4321 cttcgggtcc gtcttgggcg atcaggacgg aaaagccggc atcttttagc acccctgaga
     4381 gggtcctcag tatgctgggc tcatcatcta caacgaggat cgtatcgctc atgtttgagt
     4441 cacgggcagc tcgataatca cggtagcacc ttttggaacg ttgagggtga ttcggatctg
     4501 gccgttgtga tcattcacga tccggttgac aatcgccaat cccaggccgg ttccgctttt
     4561 tttcctggag aaatacggga ggaacagttt atcgaaatct tccggatcaa tgcctgcacc
     4621 gttatccgcg atctccaatc ggattcgatg ccgctttgga tcgtacagag tcgagatgat
     4681 cagactccct ccgccgttca tcgcttcgat cgcgttttcg aagatgttga caaaagcccg
     4741 cttccactgt tcccgatcca gcatcagggt cgggagatcc tctgcgtatc gggtttcaaa
     4801 ctccacatcg cggtgcgctg tcttgtagag acggatcact tcgtttagga tctcgtggag
     4861 attctgtgga ctggggcgcg gagacgggaa acgggcgaaa tttgagaact cgtccaccag
     4921 cgtcttgaga ctgttgacct cattgatgat cgtgcccgtg gcttcatcga agattccgtc
     4981 gaaatccggt gaatgctcaa agaacttttt acgcaaacgt tcggtcgata gctgaatcgg
     5041 cgtcagaggg ttcttgatct cgtgagcgat cctctgagct acctcctgcc aggcggccac
     5101 cttctgggcc ttgagaagtt cggataaatc ttcaaacacc atcacgacgc ctagagatcg
     5161 gccttcggga tctctcaagg cagagagatt gaatcgcaat gtgaggggtt tcttcattac
     5221 ctccagatgg atctcttcct tcagatcatc ttttcctgac cgcttaaatt tatctaaaag
     5281 cgtcataagc cggtcgagtt gacggctttg aaaggcatcc cgggcgctct tgtccaggat
     5341 ctcctctgcc gtgagtccca gaatttgctc cgcagagcga ttgcaattcg taattcgtcc
     5401 ctgagcatcc agggctatga cacccgtggc gatctgctca aggatcgttt ccatgtaagc
     5461 ccggcgccga ttgatctcga tattcgattg actcaaagag aggttggcct cctccagctg
     5521 ggtctttccg gtcttcaggt ccgccgtcat cttgttaaag gaatccacca gcatgccgat
     5581 ctcgtcgttg gccttgacct tgatccgaaa gtccaggttt ccctgggcca cagcttgagt
     5641 tccttcggcg agtttggtga tcgggacagt gatacttttg gccaggtaga agccgaacc
//
LOCUS       DATMKH010000529         1081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1211952, whole genome shotgun sequence.
ACCESSION   DATMKH010000529 DATMKH010000000
VERSION     DATMKH010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1211952"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..258
                     /locus_tag="VNV63_06315"
     CDS             10..258
                     /locus_tag="VNV63_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62270.1"
                     /translation="MMQLCGPPLCPVIEIHVIDMKNNIIIFSPPEDRQEIRNHGSWIR
                     IEHEAVSSLVESLLVLWLGAWSLFAGIEFLRSTGILLF"
     gene            287..841
                     /locus_tag="VNV63_06320"
     CDS             287..841
                     /locus_tag="VNV63_06320"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXC62271.1"
                     /translation="MIAAATLSLSQFHDRAGLGLDIEMAEEKEFEMFIRSYQNMVFSV
                     SARIVGNLTDAEDITQEVFLKAYDRFTELRTSGTVGGWLKTVATNLSINHLNRYRSRW
                     RFFSEQTEPVEIAAEAEPESDRREALELAIRKLPDPQRVPLVLFHFEEMSYEEIAAKL
                     QVSLAKIKSDIHRGRETLKRLLTK"
     gene            838..>1081
                     /locus_tag="VNV63_06325"
     CDS             838..>1081
                     /locus_tag="VNV63_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62272.1"
                     /translation="MSDPNKDWLDEELEHLRDLEAPATLLPNVMKKVRQRAGRLWWVR
                     LVEPRAELLRSLVVGVSLLMLVLLLVVDPTQFFSHLP"
BASE COUNT          251 a          275 c          297 g          258 t
ORIGIN      
        1 tgagaaaaaa tgatgcaact ttgcggccct ccgctgtgtc ctgtcattga aatccacgta
       61 atcgatatga agaacaacat catcattttt tcgccgccgg aggacaggca ggaaatcaga
      121 aaccacggca gttggatacg gatcgagcat gaggccgtct ccagcttggt cgaatcgctc
      181 ctggtcctct ggctgggggc atggtccctt ttcgctggaa tcgagttcct ccgatccacc
      241 gggatattgc tattttagtc gagcgtcccg aaggcttcgc gctaacatga tcgcagccgc
      301 aacgctctcc ttgtctcaat tccatgatcg ggcgggtctt gggttagaca tagaaatggc
      361 ggaggaaaag gaatttgaaa tgtttatccg gagctaccaa aacatggtat tttcggtttc
      421 cgccaggatc gttggcaact tgaccgatgc ggaagacatt acccaagagg tttttctcaa
      481 ggcctacgac cgattcacgg aattgcgaac cagcggcacg gtcggaggat ggcttaagac
      541 tgtggccacg aatctttcga tcaatcatct caatcgttac cgctcccggt ggcgattttt
      601 ctctgaacag actgaaccgg tcgagatcgc tgccgaagcc gaaccggaaa gcgaccgaag
      661 agaggcgttg gagctggcga tccgaaaact ccctgacccg cagcgggtgc ctctcgtcct
      721 attccatttt gaggagatga gctacgagga gatcgccgcc aaacttcaag tctcgctggc
      781 caagataaaa tccgatatcc atcgaggacg cgaaactttg aaaaggttgc tgacgaaatg
      841 agcgatccaa acaaagactg gcttgacgag gagctggagc accttcgaga cttggaggcg
      901 ccagccactc ttttgcccaa tgtgatgaag aaagttcgtc aacgggctgg gagactttgg
      961 tgggtgcgcc tggttgaacc ccgagccgaa cttttgcggt cactggtggt cggagtttcc
     1021 ctgttgatgc tggtgctttt gctggtcgtc gatccgactc agtttttctc tcatttgccc
     1081 g
//
LOCUS       DATMKH010000530         1655 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3754741, whole genome shotgun sequence.
ACCESSION   DATMKH010000530 DATMKH010000000
VERSION     DATMKH010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3754741"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1428)
                     /locus_tag="VNV63_06330"
     CDS             complement(<1..1428)
                     /locus_tag="VNV63_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62273.1"
                     /translation="MRNNTSSAYPQKPVSAAWSKVRVQIKRYYSVLNRFGWLLPLTIS
                     LGICLAAWIVAQMPPDYISQGRMQVAGQFQIKEGAVYSEEADNYYGTQIQLMQDLEVQ
                     KGAMIRVQTLHPELHPEVVMLTVNQEPRASIFDLKVESETAAYAKAYLDGIMDEYIEL
                     KRKQRLQSSDNTTLAIQDQLAHLEKDIDATDVEMHDFQEKNNLGFLEKQGNDAADYLA
                     QKTRELANLKSEYNLLGMLDLDANLDRQQANSDAAQSSANADAGGSQDNQALASMGPI
                     AEYQKAKQELELLKAQRQDLSKNLRPAHPTIMALDREIEKENNLIQTLRAESADALKT
                     RRDSIQVQIQNDQNVITEWQAKALDLSSRIAEFNRIKTKSDRSKEEYEGLLKSMTSVA
                     VTKNIDQDQVSVMDRASDAISNKPGWMNIIAIGFAGGLFVGLAILFCVDQFDDRMSSF
                     LELQTHFQEIILGQIPRDDFKGDAAL"
     gene            complement(1435..>1655)
                     /locus_tag="VNV63_06335"
     CDS             complement(1435..>1655)
                     /locus_tag="VNV63_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62274.1"
                     /translation="ENLTVSQAILQDGGLADFADKRKVKLVHKKPDGTTQTTIIDLKE
                     VLEKGHSELDPVVQPEDKIIVPQRLINF"
BASE COUNT          324 a          443 c          446 g          442 t
ORIGIN      
        1 caaggcggcg tctcccttga agtcgtcccg tggaatctgc ccgaggatga tctcttgaaa
       61 atgggtctga agttcaagaa acgaactcat gcgatcatca aactgatcaa cgcaaaagag
      121 gatcgccagg cccacgaaaa ggcctcctgc gaaaccgatc gcgatgatat tcatccatcc
      181 cggcttgttg gatatcgcgt ccgaggcgcg atccatgacc gagacctgat cttgatcgat
      241 atttttcgtc acggccacgc tcgtcatgct tttgagcaat ccctcgtatt cttccttgga
      301 gcgatccgac ttcgtcttga tgcggttgaa ttcggcgatg cggctgctga ggtcgagcgc
      361 cttggcctgc cattccgtga tgacattttg atcattttgg atttggacct ggatcgaatc
      421 ccggcgtgtt ttcagggcat cggcgctttc cgcgcgcagg gtctgaatca gattgttctc
      481 cttctcgatt tcacggtcca gagccatgat ggttggatga gccgggcgca aatttttgga
      541 tagatcctgc cgctgggcct tcagcagttc caattcctgt ttcgcctttt ggtactcggc
      601 aatgggtccc atgctggcga gggcctggtt atcctgactt ccccccgcat cggcatttgc
      661 ggagctctgg gccgcgtcgg aattggcctg ttgccggtcc aggtttgcat ccaggtcgag
      721 cattcccagg agattatatt cgcttttcag gttggccaat tctcgtgtct tttgggcgag
      781 gtaatcggcg gcgtcatttc cctgtttctc gagaaacccg agattgtttt tctcctgaaa
      841 gtcgtgcatc tccacatcgg tggcatcgat atccttttcc aaatgggcga gctgatcctg
      901 aatggccagg gttgtattat cggacgactg caggcgctgc tttcttttca gctcgatgta
      961 ctcgtccatg ataccgtcga ggtaagcctt ggcgtaggcg gccgtttcgc tttcaacttt
     1021 caaatcgaaa atggaagcac gcggctcttg attgaccgtc agcatgacca cttcagggtg
     1081 cagttccgga tgaagagtct gaacacggat catggcgcct ttttggacct cgagatcctg
     1141 catcaattgg atttgagtac cgtaataatt atccgcctcc tcactgtaga ccgcaccttc
     1201 cttgatctga aactgcccgg cgacctgcat tcgcccctgg gaaatatagt cgggaggcat
     1261 ttgcgccaca atccacgcgg ccaggcaaat gcccaggctg atggtcaggg gaagcagcca
     1321 tccaaaccga ttcaggaccg agtagtaccg cttaatctgg acccggactt tggaccaggc
     1381 ggcggaaacc ggcttttggg gatacgctga ggaggtgttg tttcgcatgg caggtcagaa
     1441 attgatcagc ctttgcggaa caatgatctt atcttcaggc tgcaccacgg gatcaagttc
     1501 cgagtgccct ttttccagga cttccttcaa atcgatgatc gttgtctggg ttgtgccatc
     1561 cggcttctta tggaccagct tgaccttgcg cttgtcggca aaatcggcca ggccgccgtc
     1621 ttggagaatc gcctggctta cggtcaagtt ctcgt
//
LOCUS       DATMKH010000531         2167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3123137, whole genome shotgun sequence.
ACCESSION   DATMKH010000531 DATMKH010000000
VERSION     DATMKH010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3123137"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="VNV63_06340"
     CDS             complement(<1..727)
                     /locus_tag="VNV63_06340"
                     /inference="COORDINATES: protein motif:HMM:TIGR03187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DGQHR domain-containing protein"
                     /protein_id="HXC62275.1"
                     /translation="MPLRVPAIRFQQKGVPMYLAALPIDELDFCSIDQWDPKKTGKWK
                     GYQRGLVQKKIRNLARYLERDDAILPVAGLLNLRKSGTVKFSGAQGHKAAMGELVIPD
                     DTRLWVVDMQHRLEGIKTAHSEGFLKDFLVPVLITEGLSAIREAAQFYVINTRSRRMG
                     VDLTRRLLIEHDEIKDLADVPDWELKAVRIAIQLNLRIKNNPWYGRIREPEGEGSILH
                     IATEKSFVPSLRWLLTGPKAKRKS"
     gene            complement(904..2031)
                     /locus_tag="VNV63_06345"
     CDS             complement(904..2031)
                     /locus_tag="VNV63_06345"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HXC62276.1"
                     /translation="MTKSPIKLPSPVFNDEKNHIRLYQRDSLELLALLPESSVDLIFA
                     DPPYFLSNGGITCHAGRMVSVNKGEWDKSKGPDANHDFNRAWLAACQRILKENGSIWV
                     SGTSHVIHSVGFAMQQLGFKLLNDISWVKPNPPPNLSCRYFTHATETIIWAAKNKKSR
                     HTFHYKLMKEINLGKQMKSVWEIKRPESWEKKFGKHPTQKPLALLERILLASTNESDL
                     VLDPFNGGGTTLVSAFRLRRHAFGCELSLDSIELSVRRICSELAHVTLSFSILDFSVD
                     LFSRSMDRSDRFTLAKLSSRAKRGICFSPSAAERAYVPNHFQLVQQERRFYFIGRTDR
                     RVLFSVLASSLEDAHAKFLASGHSSSDALFIIKTETEIYLA"
BASE COUNT          493 a          452 c          723 g          499 t
ORIGIN      
        1 ctgatttgcg cttcgccttt ggtccggtga ggagccatcg gagggatggc acgaatgatt
       61 tttcggtcgc aatgtggaga atgctacctt ccccttctgg ctctcgaatt ctgccgtacc
      121 atggattgtt tttaattcgc aagtttaatt ggattgcgat tcggaccgct ttcaactccc
      181 aatctggtac gtcggctagg tccttaattt cgtcatgctc gattagcaag cgacgagtca
      241 ggtcaacacc cattcgtctt gatcgcgtgt taataacgta aaactgggca gcttcgcgga
      301 ttgcactgag cccttccgtg atgagaaccg gaacaaggaa atcctttagg aagccctcgc
      361 tatgtgcggt cttgatgccc tcaagcctgt gctgcatgtc tacaacccac aagcgcgtgt
      421 cgtcaggtat tacgagttct cccatcgcgg ccttgtgccc ttgagcgccg gaaaacttca
      481 cagtgcctga tttgcggaga tttaggaggc ctgcgacggg aagaattgca tcgtcgcgtt
      541 caaggtagcg ggccaggttc cgaatcttct tttggacgag tcctctctga tagcccttcc
      601 actttcctgt cttttttgga tcccactgat caatggagca aaaatcaagc tcgtcgatcg
      661 gcagcgccgc cagatacatt ggcacgccct tttgctggaa gcgaatcgcg ggcacccgta
      721 agggcatgga atcctccagg agaagctctg agggtccggg cagacttggt tatgaagcac
      781 tatccttaaa agctgactag cttgaacgac ggtcttcaga gccttgcaag gtcaggggcg
      841 cattagatca cgggatgatc gggatggcaa ggcactagca ggtagctggc gctccggcta
      901 attttacgcc agatagattt cggtttcggt tttgatgatg aagagggcgt cggaggagga
      961 gtggccggag gcgaggaatt ttgcgtgggc gtcttcgagg gatgaggcga ggacggagaa
     1021 gaggacgcgg cggtcggtgc ggccgatgaa atagaagcga cgttcttgtt ggacgagttg
     1081 gaaatggttg ggcacgtagg cgcgttcggc cgcggacgga gaaaagcaga tccctcgctt
     1141 cgctcgggat gacaattttg cgagcgtgaa gcgatctgat cgatccatag atcgcgagaa
     1201 gagatcaacg gaaaagtcga ggatggagaa agaaagtgtg acgtgtgcca attcggaaca
     1261 gatacggcgg acggaaagtt cgatggagtc gagggagagt tcgcagccga aggcatgacg
     1321 acgcaggcga aatgcgctga ctagtgttgt gccgccgccg ttgaaggggt cgaggaccaa
     1381 atcgctttcg ttggtggagg cgagaaggat gcgttcgagg agggcgaggg gtttttgtgt
     1441 gggatgtttg ccgaatttct tctcccagga ttcgggccgc ttgatttccc agacggactt
     1501 catctgtttg ccaagattaa tttccttcat gagcttgtag tggaaggtgt ggcgggactt
     1561 tttgtttttg gcggcccaga tgatggtttc ggtggcgtgg gtgaaatagc ggcaggaaag
     1621 attgggaggc ggattgggct tgacccagga aatgtcattg agaagcttga agccgagctg
     1681 ctgcatggcg aagccgacgg agtggatgac gtgagaggtg ccggaaaccc agatggagcc
     1741 gttttctttg aggatgcgct ggcaggcggc aagccaggcg cggttgaagt catggttggc
     1801 gtcggggcct ttggatttgt cccactcgcc tttgttgacg gagaccattc tccccgcatg
     1861 gcaggtgatg ccgccattgg agaggaagta cggcgggtcg gcgaagatga ggtcgacgga
     1921 ggattcgggg agaagggcga ggagttcgag ggagtcgcgc tggtagaggc ggatgtggtt
     1981 tttttcgtcg ttgaagacgg gagaggggag tttgatgggg gatttggtca tggaggattg
     2041 atcgatcagg atcgttgatc aggtgggtac gggtgtcaag gaggagtaga aggaaggaat
     2101 gaagtaaaga agtaaggaag caaagaagca aagaagcaaa gaagcaaaga agcaaagaag
     2161 caaagaa
//
LOCUS       DATMKH010000532         1937 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3123709, whole genome shotgun sequence.
ACCESSION   DATMKH010000532 DATMKH010000000
VERSION     DATMKH010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3123709"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="VNV63_06350"
     CDS             <1..609
                     /locus_tag="VNV63_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62277.1"
                     /translation="PQHQMAWAIKEGETLSSQTFSDLSHEITHIYFIDYMEDKGGMHQ
                     GHAWLHEAVACYSERDPYRKNREQWARDHMNDRIPFEQLFTMKNPQKENPLVELTVKL
                     HEKLARGEIKVEELNRQISEFATAHGAELGQAGIRNMTYYSESLSIFEFLLKTEGKEF
                     IRAMCQALKKGKSMDEIIRGLKAYPKGLPQLEQAWMAWVQSS"
     gene            626..982
                     /locus_tag="VNV63_06355"
     CDS             626..982
                     /locus_tag="VNV63_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62278.1"
                     /translation="MYLEIDENPNYPSSVSMRFKELGPAREIKQLRLSDRKSEEALCW
                     VTGWCDDPSQPFCPVYAQQVEDSGQGLAYLIYGGNWGIRVKSVNLEEDWDLYSTNQWG
                     EAFLLISDPQSIMYAD"
     gene            complement(1030..>1937)
                     /locus_tag="VNV63_06360"
     CDS             complement(1030..>1937)
                     /locus_tag="VNV63_06360"
                     /inference="COORDINATES: protein motif:HMM:NF014801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HXC62279.1"
                     /translation="SLEWDQELLRLFEIPEAILPRLLPTTASYGEAVLGKRRIPILSS
                     IGDQQGSLCGQGGFSSGDMAVTYGTGGFLVFNTGDQLPAQSQLLKTIAWSSQDKIHYA
                     LEGTVNAVGSAILWLQNNLGLITHPSEIDRLCKQAKGRAWMIPAISGLGSPHYSQTDT
                     AFLGLDRKTTKADLVKSLIEGIAYLMKDNFEQIKRGSPIPIRKIIAGGGAARNAYLIQ
                     FQADLFQKEVAVASVYETSSRGAAYLAGLGCGFWKNLDGIQHLKQTRKVFQPRLVPRE
                     VERLYARWQEALHLGQAWSEKKERS"
BASE COUNT          464 a          515 c          537 g          421 t
ORIGIN      
        1 ccgcagcatc agatggcctg ggcgattaaa gagggcgaga cattatcaag ccagaccttt
       61 agcgacctct ctcatgagat cacgcacatc tactttattg actacatgga agacaaagga
      121 gggatgcatc agggccatgc ctggcttcat gaagcggtgg cctgctacag cgaacgggat
      181 ccttatcgaa aaaaccgcga gcaatgggcg cgcgatcata tgaacgaccg cataccgttt
      241 gaacagctct ttacgatgaa aaacccgcaa aaagaaaacc cgctcgttga gctgaccgtg
      301 aagctgcatg aaaaactggc ccgcggcgag atcaaagtgg aggagttgaa ccggcagatc
      361 tccgaatttg ccacggcgca cggcgcggag ctgggccagg ccgggattcg gaacatgacc
      421 tactattcag agtcgctgag catcttcgag tttctgctca agaccgaagg caaggagttc
      481 attcgggcga tgtgtcaggc cctcaaaaaa gggaagtcta tggatgagat catccgtggt
      541 ttgaaggcct atcccaaggg ccttccgcag cttgagcagg cctggatggc ctgggtgcag
      601 agttcttaac ccaatttact aaaaaatgta tcttgaaata gacgaaaatc ccaattatcc
      661 tagctcggtc tcgatgcgtt ttaaagagct gggaccggcg agggaaatca aacaactccg
      721 gctcagcgac cgaaaaagtg aagaggcgct ctgctgggtc accggctggt gtgatgatcc
      781 gagccaaccg ttctgcccgg tctatgcgca gcaggtggaa gactccggac agggactcgc
      841 ctatcttatc tacggcggca actgggggat ccgggtcaag tcagtgaatc tcgaggagga
      901 ctgggatcta tatagtacta atcaatgggg cgaggccttc ctgctgatca gcgatcctca
      961 aagtattatg tacgcggatt aacactttcg cttgtttaca tccccggttg gaacttgtgc
     1021 atgccgccat cacgaccgct cctttttttc agaccaggcc tgccccaagt ggagcgcttc
     1081 ctgccagcgg gcgtagagcc gctcgacctc gcgaggcaca agccgcggtt gaaagacttt
     1141 acgagtctgc tttagatgct ggattccgtc tagattcttc caaaagccgc atcccagacc
     1201 ggccagataa gcggcgcctc tcgaactcgt ctcatagacc gaggcaaccg caacctcttt
     1261 ctgaaaaaga tcagcctgaa actgaatgag ataagcgttt ctggccgcgc cgcctccagc
     1321 gataatcttc cggatcggaa tggggcttcc tcgcttgatc tgctcgaagt tgtccttcat
     1381 cagataggcg attccttcga ttaagctctt cacgagatcg gccttcgtgg ttttgcggtc
     1441 aagacccaga aaggcggtat cggtctggct gtaatgaggt gagcccagac ccgagatggc
     1501 cgggatcatc catgctcttc ccttggcctg cttacagaga cggtcaatct cgctgggatg
     1561 cgtgatgaga cccagattat tttggagcca tagaattgca gagcccaccg cgttcaccgt
     1621 tccctccagg gcataatgga tcttgtcttg ggaagaccag gcgatggtct ttaaaagttg
     1681 agactgtgca ggcagttgat cgccagtgtt gaatacgaga aatccgcccg tgccgtacgt
     1741 gaccgccatg tcgccggatg agaaaccacc ttgaccgcag agcgagccct gctgatcgcc
     1801 gatgctggat aagatcggga tacgacgttt gccgagcacc gcctctccgt aggatgccgt
     1861 ggtcggcagc aggcgcggca ggatcgcctc gggaatctca aataagcgaa gcagttcttg
     1921 atcccattcc aggcttt
//
LOCUS       DATMKH010000533         1242 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3757043, whole genome shotgun sequence.
ACCESSION   DATMKH010000533 DATMKH010000000
VERSION     DATMKH010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3757043"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..913)
                     /locus_tag="VNV63_06365"
     CDS             complement(<1..913)
                     /locus_tag="VNV63_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXC62280.1"
                     /translation="MNEERAPEYGGFGSEPSTPLEFLERIIASLPPGDPTRSELIDLR
                     SNFAEQEEMISEARHTIEKLDEVIKKVTSPANRIGTYLGTPNKETAQIVVGGSDYYCN
                     VDPRINLSRLKKGTRVLVNEAYVIVGDLGYDLSGPVTKITELLGADRLRVGMEHGMQA
                     VVLQRCQDLMKETLKVGDEIRVDPNYRVAIEVLSSSKSEEYYLETVPELPWEKVGGQE
                     EALSAIKDAIELPLLHADLFAKFQHQTPKGFLLYGPPGCGKTLIGKATAYNLTRQLKE
                     KTGEDMQQFFMHIKGPEILNMWVGESER"
BASE COUNT          281 a          344 c          289 g          328 t
ORIGIN      
        1 tgcgctcgga ctcgcccacc cacatgttca agatctccgg tcccttgata tgcataaaga
       61 attgctgcat atcttccccg gttttttcct tcaattgccg ggtcaaattg taggctgttg
      121 cttttccgat caaagtctta ccacaaccgg gcggtccgta gaggaggaaa cctttggggg
      181 tctggtgttg aaactttgca aagagatctg cgtgcaaaag aggcaattcg atcgcatcct
      241 taatggcact gagagcttcc tcttgcccac cgaccttctc ccacggcaac tcgggcaccg
      301 tctcgaggta atactcttcg gatttcgatg aactcaagac ctcaatggca acccgatagt
      361 tggggtcgac ccgaatttca tctccgactt tcagcgtctc cttcatcagg tcctggcatc
      421 tctgcagcac gaccgcctgc atcccatgtt ccatgccgac tcggagacgg tccgctccaa
      481 gcaattcagt gatcttggtg accgggcccg ataaatcgta cccaaggtcg ccaacgatca
      541 cgtaggcttc attgaccaga acccgcgttc ctttttttaa tctcgaaaga ttgattctcg
      601 ggtcgacgtt gcagtaatag tcagaacctc ccaccacaat ctgagcagtc tccttgttgg
      661 gggtgccgag ataggttcca atgcgattcg ccggggaagt cactttcttg atgacttcgt
      721 cgagtttttc gatcgtgtgc cgagcttcgc ttatcatctc ttcctgctcg gcgaagtttg
      781 accgcaaatc gatcagttcg cttctagtag ggtcacctgg agggagactg gcaatgattc
      841 tctccaggaa ttcgagagga gttgagggct cagaaccaaa accaccatat tcgggtgctc
      901 tctcttcgtt catctgtact tcttaatcag tccgcgttgc gactataaac ggattccgaa
      961 ttcgtagcaa acctggactt gaacctgtca ccgaatttca ttcgacccat acgtttctcg
     1021 acgtcatcca cagtaaatct attcaatcac ctgtcaggaa agcgtcccgc ctttccaccg
     1081 gcgatccaac tagccgaaag aatgttatgg cttccaggcg gcacactgct ttgcgggaaa
     1141 taccccgggc aaggcgcggt atcgtcatga ttaagaaagc gttgcatgat tttcgggatt
     1201 actgatattg atcgcttatg ctagacaccc tcaaggagcc aa
//
LOCUS       DATMKH010000534         1066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4422225, whole genome shotgun sequence.
ACCESSION   DATMKH010000534 DATMKH010000000
VERSION     DATMKH010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4422225"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(303..551)
                     /locus_tag="VNV63_06370"
     CDS             complement(303..551)
                     /locus_tag="VNV63_06370"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXC62281.1"
                     /translation="MASALPPKSNGNNVLVVDDDNEVCQIIQRMLSYEKYQVQTSQSV
                     ADALRAIEQKPFDAYAMDDKLLDGSGLDVAERIRSKGR"
     gene            complement(815..>1066)
                     /locus_tag="VNV63_06375"
     CDS             complement(815..>1066)
                     /locus_tag="VNV63_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62282.1"
                     /translation="QAPMATPRRELDHSQPAGQRRTRESDEFVREIHTRMPVILPEVH
                     HACWLSGEAGKEVLVLVPLTRRTELGVADYSPREFTEKQ"
BASE COUNT          240 a          285 c          274 g          267 t
ORIGIN      
        1 aggagggtct gatgccgaca gacggaagat ctcgtcggca tccagaacga aaccactggc
       61 gaatggaatt tggtccctga gttcacccct ccggttttcg cctcgtgacc gatcgtatta
      121 aatgtcggtt accgggttct gatcaggcca atgcggcctt agttgatccg gtcgccttcc
      181 ccagtgcgct acagatcatc tcccgcgaga aaggtttttg gaggaaatca aatatacgaa
      241 atttctccgc tctcaacgcg atggcgcttg cgtcataacc cgacataaga atgatcggag
      301 cttcacctcc ccttcgaccg aatccgctcc gcgacatcga gtccggaacc gtccagcaac
      361 ttgtcgtcca tcgcataggc atcaaaaggt ttttgctcga tggctcggag agcatcggcg
      421 acagattggc tggtctggac ctgatatttt tcataggaaa gcatccgttg aatgatttga
      481 cagacctcgt tgtcgtcgtc taccactaag acgttattcc cattggattt gggcggcaga
      541 gccgatgcca caggtggagc agtggaagtt gaatttcttc cggagatgtg tcaaaagccc
      601 tttcatccgg tacattatca caagtatggt aattattcta tttatattac ggagcgacct
      661 attcgccgca cggttgaaag ccgagacaac gacttcagcc agtttctttg gtcgcggtct
      721 tttgggacat gccatcaaag aagttgcgga agattttcct gtcgcgccac ggattctacc
      781 tcgaccggcg cgaaaaaaaa acttgatccc ggcgtcattg tttttcggtg aattctctgg
      841 gcgagtaatc ggccacgcct aattcggtcc gccgggtaag ggggaccaag accaaaacct
      901 cttttccagc ttcaccagat aaccaacaag cgtgatgcac ttccggcaag atgaccggca
      961 tccgggtgtg gatctcccgc acaaactcgt ctgattctct ggttctccgc tgaccggctg
     1021 gttgcgaatg gtccagttcc cgtcgtgggg ttgccatcgg agcctg
//
LOCUS       DATMKH010000535         1673 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_251802,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000535 DATMKH010000000
VERSION     DATMKH010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251802"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1110)
                     /locus_tag="VNV63_06380"
     CDS             complement(<1..1110)
                     /locus_tag="VNV63_06380"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HXC62283.1"
                     /translation="MEFTLSLTAQDMALLLPEFFMTVLLTVVLFLDFIFPRMNKNILA
                     YLSIAGMIGMIALLVGYFIDGTTGTLFKQMYVLDPLAIAFKIFILFATVLVFLASIQY
                     LKKIPAFKGEYYFLILLSALGMMLMASANDLLSLFITVEFSTFNFYVLVAYHRDDLKS
                     SEAGLKFFILGVFTAAILAFGISLVYGETGTLIFSEMVQKMPSVTPGLVIGFLLIIMA
                     LAFKIGAVPFHVWIPDIYEGAPTPITALLSIAPKGAAFAIILRVLFSTLGDFKVDWVW
                     FIAVLSALSMTYGNIVAISQTNIKRLLAYSGIAQIGNLLIGVAAGTKMGGDSLLFYLL
                     TYLFASVGAFTVVIIFSNLTDSDDIEDYNGLSRRSP"
     gene            complement(1112..>1673)
                     /locus_tag="VNV63_06385"
     CDS             complement(1112..>1673)
                     /locus_tag="VNV63_06385"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HXC62284.1"
                     /translation="LGMASLNVISMTGAVLYMFAHAIATGMLFAIAGYVYDQTHTRDI
                     SSLGGLSSKMPFMTAIFILAVGASFGLPMTVNFIGELMILAGSWAVYPVQTIIAVLGI
                     ALTLAYLIRLLRGVFFGPMNPQYAHVRDASPFVDRLPLLVLAATSLYFGLFPSQFIHV
                     IQAGVTPLIAKIQNFAPMVSQQGGPF"
BASE COUNT          452 a          450 c          464 g          307 t
ORIGIN      
        1 cggcgaacgc cggctcaaac cgttatagtc ttctatgtca tcgctgtcgg tcaggttcga
       61 gaagatgatc acaacggtga aggcgcccac actcgcaaag aggtatgtca agagatagaa
      121 gaggagggag tccccgccca tcttcgtccc ggccgccacg ccgatcagaa ggttcccgat
      181 ctgggcgatt ccggagtagg ccaggaggcg tttgatattc gtctgagaga tcgccacaat
      241 gttcccgtag gtcatcgaga gggccgacag caccgcgata aaccagaccc agtcgacttt
      301 gaagtcccca agcgtagaaa acaggacacg cagtatgatc gcaaacgcgg ctcctttcgg
      361 ggcgatcgaa agcagagcgg taatcggcgt cggcgcccct tcgtagatgt ccggtatcca
      421 gacatgaaaa ggcaccgccc cgatcttgaa agccagcgcc atgatgatca aaaggaatcc
      481 gatcaccagc cccggcgtca cggagggcat cttctggacc atctcagaga agattagtgt
      541 ccccgtctct ccatagacga ggctgatccc gaaggccagt atcgcggcgg taaaaactcc
      601 gagtatgaag aacttcagtc ccgcctcgct ggacttcaga tcgtccctgt gataggcgac
      661 gaggacataa aaattaaagg ttgagaactc aaccgtgata aagaggctca gaaggtcatt
      721 ggccgaggcc atcagcatca tacccagggc cgataggagg atcagaaagt agtattctcc
      781 tttaaaagcc gggatttttt taagatactg gatggaggcc agaaacacca ggaccgtcgc
      841 aaagaggatg aagatcttaa aggcaatcgc cagcgggtcc aggacgtaca tctgtttaaa
      901 gagcgtgccg gtggttccgt cgataaagta tcccaccagc agggcgatca tcccgatcat
      961 tccagcgatg ctcagatatg ccagaatatt tttattcatc cgcggaaaga taaaatccag
     1021 gaacaggacc acggtaagca gtacggtcat aaaaaattcc gggagcagca gggccatgtc
     1081 ttgtgcggtc aggctgagcg tgaactccat gtcagaacgg gcctccttgc tgtgaaacca
     1141 tcggcgcaaa attttggatc ttggcgatca acggcgtgac cccggcctgg atcacatgga
     1201 tgaattgcga cgggaacagg ccgaagtaca agctcgtggc agccagtacc aggagcggca
     1261 ggcgatcaac aaaaggactc gcgtcccgta catgtgcata ctgaggattc atcggtccga
     1321 agaagacacc gcgcagcagc cggatgaggt aggccagagt cagcgcaatg cccaaaaccg
     1381 cgatgatcgt ctggacggga taaaccgccc agctccccgc caggatcatg agttccccaa
     1441 taaaattcac cgtcatcggc agaccgaacg acgccccgac cgcgagaatg aagatagcgg
     1501 tcatgaacgg cattttggat gacagtcctc caagcgatga gatgtcgcgc gtgtgggtct
     1561 gatcgtaaac atatcccgca atggcaaaca acatcccggt ggctatcgca tgggcaaaca
     1621 tgtacagcac cgcgccggtc atactgatca cgttcaagga cgccatcccg agg
//
LOCUS       DATMKH010000536         1370 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4422842, whole genome shotgun sequence.
ACCESSION   DATMKH010000536 DATMKH010000000
VERSION     DATMKH010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4422842"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..850)
                     /locus_tag="VNV63_06390"
     CDS             complement(158..850)
                     /locus_tag="VNV63_06390"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HXC62285.1"
                     /translation="MEIESTYIDGRGWRDSRWLVLVEFGLVVAIYNGRQHHILKVSAT
                     PYLLLLAWISLRLRRVQWKQIGFTRYRTWAKTLLLGITFGVGLEIFDLFGKQPLLTRL
                     LGKPPDLSNFLAVRGNLKFALVMIGLIWILAAFGEELVYRGYMMNRIADLGRGTRTAW
                     FVSLCLVSALFGFSHYQQGITGIIEEGSDGLILGLMYLGCRRNLAVPIVAHGVCDTID
                     ITLLFLGKYPGL"
     gene            complement(1123..>1370)
                     /locus_tag="VNV63_06395"
     CDS             complement(1123..>1370)
                     /locus_tag="VNV63_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62286.1"
                     /translation="SLRDARTNTGNFSLFKDFKVKERLNVTFHTTMTNVFNHPNFGTI
                     DPFLDDAGLQQEFTGFGDQTLQSGGIRSIRFGLTFKF"
BASE COUNT          353 a          346 c          380 g          291 t
ORIGIN      
        1 acccgcgctc ctccgtagcg cgtcgggaga gcttttgccc tcttgacaag gacctatcat
       61 ggaagtcgga aaaccacaaa agttccttcc aagtacttaa catgaacggt tactacgttt
      121 atcagatctc agccgattta gtaagtgatt ggcggcccta caggccggga tacttgccca
      181 agaaaagtag ggtgatgtcg atggtgtcgc agactccgtg ggcgacaatt ggaacggcga
      241 ggttgcgacg acagccgaga tacatcaatc ccaagatcag cccgtcagaa ccctcctcga
      301 tgatgcccgt gatgccctgc tgataatgag agaaaccaaa gagggcacta accaggcata
      361 aactcacgaa ccatgcggtg cgggtaccgc ggccgaggtc ggcgatccga ttcatcatat
      421 agccacgata gacgagctct tctccaaacg ccgcaaggat ccaaataagg ccgatcatga
      481 ccagggcaaa tttgaggttt cccctgactg caagaaaatt cgagaggtcg ggcggctttc
      541 ccagaaggcg ggtgagaagc ggctgcttgc cgaacagatc gaagatctcc agtccgacgc
      601 caaaggtgat gccaagcaga agagtcttcg cccacgtgcg ataccgtgtg aagccgatct
      661 gtttccattg gaccctgcga agacgaagcg aaatccatgc gagcaggagg agatacggcg
      721 ttgcgctaac cttcaggatg tgatgctggc ggccattgta gatagcgacg accaggccaa
      781 attcgacgag aaccagccat ctgctgtcgc gccaaccccg gccatcaatg taggtggatt
      841 cgatttccat ttcctcgcgc ttctccgggg caatacggtg agaagcgaga aaaagtctca
      901 gttgtgcgga agatttgtct cgtgcagagt tccaaggatg tgaggtcctg ccagccagag
      961 gataactcct gggtcgcttt accggagaag tgtcgtacta ctctttcagc gaaaccgcct
     1021 ggtgagattc cgaagcccat gctgggatgt tcggaaactg gggaaggtcg taaaagaaag
     1081 accttccctc agataaaaaa gcattcgttt aaaaacgtct gtctagaatt tgaaagtgag
     1141 cccgaaacgg atactgcgga taccgccact ctgtagtgtc tggtccccga agccggtgaa
     1201 ctcctgctgc aggcccgcat catcgagaaa gggatcgatg gtgccaaagt tcgggtggtt
     1261 gaagacattc gtcatggttg tgtggaaggt cacgttcaac ctttccttga ctttaaaatc
     1321 cttgaaaagt gaaaagttgc cggtgttggt tctggcgtcg cgcaaactat
//
LOCUS       DATMKH010000537         1225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3445640, whole genome shotgun sequence.
ACCESSION   DATMKH010000537 DATMKH010000000
VERSION     DATMKH010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3445640"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..934)
                     /locus_tag="VNV63_06400"
     CDS             complement(125..934)
                     /locus_tag="VNV63_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62287.1"
                     /translation="MNSDTAKEILSAYRSKDRDGADPTFREALRQMESDADLKKWFDD
                     QLDLDRTIREKLAAIEPPADLQAKILARIRGEKLRRSSFALPPAWLAVAACLVLGGLA
                     LFFSTRSGGSERFQEFKSDALAMVSKQGGPDLDLETPNLTETQSFLREHKAPCYEALP
                     SRLKLMETAGCRAFVWKDYPASLTCFRLPDGLFLHLVVINKAAMDNADIPAGMKAMGD
                     WHVMFQQKNGMLLMWASQAPMDQLKQMLVALRGVQGVQREPKSEEVFSVRW"
     gene            complement(937..>1225)
                     /locus_tag="VNV63_06405"
     CDS             complement(937..>1225)
                     /locus_tag="VNV63_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXC62288.1"
                     /translation="EHQDLLVSDSAVSQAIDAGTVLEVLAKIEESYRMALELFYLADL
                     SYKEIAASLDIPIGTVMSRLSRGKEQLRTVLAKTAPPDKISPSPQAKHLPS"
BASE COUNT          277 a          356 c          302 g          290 t
ORIGIN      
        1 tgaacctgtt ccaggcaatg aattgcctgg ctacgatcat ctcatccctt cgagacaata
       61 ccctcaacac ctgtctacga attcggcgcc agatcactct gatggagtac tgggcagttc
      121 gtcgctacca acgtacggaa aacacttcct cgcttttggg ttctctttgg accccttgca
      181 ctcctctcaa cgccaccaac atctgcttga gttgatccat cggcgcttgg ctagcccaca
      241 tcaacaacat cccgttcttt tgctggaaca tcacgtgcca atctcccatc gccttcattc
      301 cagcagggat atctgcgtta tccatggcgg ctttgttgat gactaccaag tgcagaaaca
      361 gaccgtccgg cagacggaag caggttaacg acgccggata atctttccaa acgaaggccc
      421 ggcaacctgc ggtctccatc aatttcagac gggaaggcaa cgcctcgtaa caaggcgctt
      481 tgtgttcacg cagaaagctt tgggtctcag tcaggttcgg cgtttccaaa tcgagatccg
      541 gtccaccttg cttggaaacc atcgcgagcg catccgattt gaattcctgg aatcgctccg
      601 atccacccga acgggtcgaa aaaaacaggg ccaacccgcc cagcaccaga caagctgcta
      661 ccgctaacca agccggtggc agagcaaacg aggatcgccg gagtttctct cctctgatcc
      721 gagccagaat tttagcctgg agatcagccg gcggctcgat ggcagccagc ttctcccgaa
      781 tcgttcggtc caagtcgagc tggtcgtcga accatttctt gagatcggcg tcgctctcca
      841 tctgcctcag cgcctcgcga aaagtaggat ccgccccatc ccggtctttt gaccggtatg
      901 cagataaaat ctctttggcg gtatccgaat tcatgttcaa gagggcaaat gtttggcctg
      961 gggcgagggg ctgattttgt ctggtggtgc ggtcttagct aaaacagttc tcaattgttc
     1021 cttacctcgg gatagccggg acatgacagt cccaatggga atgtcgaggg aagcggctat
     1081 ctctttatag gagaggtccg ccaggtaaaa taattccaag gccattcgat aactctcttc
     1141 aatcttggcc aatacttcca gcacggtgcc agcgtcgatc gcttgtgata cggccgaatc
     1201 gctgaccagt aggtcctggt gttcc
//
LOCUS       DATMKH010000538         1052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3758310, whole genome shotgun sequence.
ACCESSION   DATMKH010000538 DATMKH010000000
VERSION     DATMKH010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3758310"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="VNV63_06410"
     CDS             <1..482
                     /locus_tag="VNV63_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62289.1"
                     /translation="RSALNLDGWTFGDIHHRASNQQIMFLYGTKHGLRPQDLSSKDPA
                     VRTEAELDVSDAKQIDASLKQYGGYRLYVDTASHMDFTDHSLVSPWRNWTEPGHISPA
                     RIQTIVRAYVLAFFDETLRGGKPGLLQSGNSSPFHEVQIEEFSPESGSTSAGSASRQ"
     gene            complement(565..>1052)
                     /locus_tag="VNV63_06415"
     CDS             complement(565..>1052)
                     /locus_tag="VNV63_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62290.1"
                     /translation="LDTRARDYIGGTAGQTPTGNSTKRGRLSGYFHRNRGHESRKAWW
                     GYFTNDLKERRVRYVTRLAAGIFPAIPAFLKNTHEINSFAARNYRVRSISDKLTLFRA
                     SKQAGDGVPHDNGWGAIFEKGIEIHEIPGDHWQVLSEPGIDVLAQSIHDCLSHFDRSL
                     V"
BASE COUNT          238 a          300 c          259 g          255 t
ORIGIN      
        1 tccgatcagc attgaacctg gacggatgga cctttggaga cattcatcat cgggcatcca
       61 atcaacagat aatgttcctc tacggaacga aacacggtct ccggccgcaa gacttaagtt
      121 ccaaagatcc tgctgtgcga actgaagcag agcttgatgt gtcggatgcc aagcagatcg
      181 atgccagtct caagcagtac ggcggctacc ggctatacgt ggacactgcc tctcatatgg
      241 acttcaccga tcactctctt gtgtccccat ggcgaaattg gaccgaaccc ggtcatattt
      301 cgccggcccg tatacaaacg attgtccgcg cgtacgtact tgcttttttc gatgagacac
      361 ttcggggagg gaaacctggt cttctccaat ccggaaattc aagcccattt cacgaagtgc
      421 agatagaaga attttccccg gaatcagggt ccacgtccgc tggctcagcc tcccgtcaat
      481 agcctgacgt acaaagacct caccgcggta cgatttcatc tctacagccg ggttcttctg
      541 tagttcatcc gaagaccctg acccttacac gagcgatctg tcaaagtgac tgaggcaatc
      601 gtgaatggac tgcgcgagca cgtcgatacc tggttccgat aagacttgcc agtgatctcc
      661 gggaatctcg tgaatttcaa ttcccttctc gaaaatggct ccccagccat tatcgtgcgg
      721 cacgccgtca cctgcctgct tcgaggcccg gaagagggtc agcttatccg agatcgacct
      781 cacgcgatag ttacgcgcag cgaaagaatt gatctcgtgc gtattcttca gaaatgctgg
      841 tattgcagga aatatgccgg cagcaagcct ggtgacgtat cgcactcggc gctctttgag
      901 gtcatttgtg aaatatcccc accacgcctt tcggctctcg tggccccggt tcctatgaaa
      961 atatccgctg aggcggccac gtttggtcga attgccagtg ggtgtttggc cagctgttcc
     1021 tccgatatag tcccttgctc tcgtatccag ca
//
LOCUS       DATMKH010000539         1892 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3125311, whole genome shotgun sequence.
ACCESSION   DATMKH010000539 DATMKH010000000
VERSION     DATMKH010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3125311"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1022
                     /locus_tag="VNV63_06420"
     CDS             <1..1022
                     /locus_tag="VNV63_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009370136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HXC62291.1"
                     /translation="YKKIRKFLSGKMIAREIVDPDSGEVLLKKKKLLTQDILGKIRDE
                     GLRNITLTDQETQTKIEEIERSTKEQMEILQTLYDEKVGRLKKGDELPPGVIKLVKVY
                     IAMKRKLSVGDKMAGRHGNKGVVSRILPEEDMPYLPDGTPVEIVLNPLGVPSRMNVGQ
                     ILETHLGWSAKALGIYVTSPVFDGATEREIKDLLKKAGLPESGQATLYDGLTGESFDR
                     PVTVGHMYMMKLHHLVDDKIHARSIGPYSLVTQQPLGGKAQFGGQRLGEMEVWALQAY
                     GAASTLQEFLTVKSDDVPGRSRIYEAIVKGENFLEPGLPESFNVLVKELQSLALDVEL
                     IKAKE"
     gene            1081..>1892
                     /locus_tag="VNV63_06425"
     CDS             1081..>1892
                     /locus_tag="VNV63_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015349106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HXC62292.1"
                     /translation="MEGIYSLFEKPKDSVSFDAIRIRIASPEKIRSWSYGEVKKPETI
                     NYRSFKPERDGLFCAKIFGPTKDWECNCGKYKRMKHRGIVCDKCGVEVIQAKVRRERM
                     GHIELAAPVAHIWFLKGVPSRIGNLLEMSLKQLERILYFESYVVTDAGETSLKEKESL
                     SEEKYRSLVQEHGDAFKADMGAEAIRELLKKINIDAMAKELHKKIKEASSTALKKKHT
                     KRLRVVEAFRKSGNRPEWMILDVIPVLPPELRPLVPLDGGRFATSDLNDLYRR"
BASE COUNT          497 a          431 c          553 g          411 t
ORIGIN      
        1 aatacaagaa gatccggaaa ttcctcagcg ggaagatgat cgccagggaa atcgtggatc
       61 ccgatagcgg ggaggtcttg cttaagaaaa agaagttgct cacgcaagat atacttggaa
      121 agatccgcga cgagggtctc agaaacatca cactgaccga tcaggaaact cagaccaaga
      181 ttgaagagat cgagcgatcc accaaggaac agatggagat cctgcagacc ctttatgatg
      241 agaaggtcgg gcgtctcaag aaaggcgatg agttgccgcc aggtgtgatc aagctggtca
      301 aggtttatat cgccatgaaa cgaaagcttt cagtgggtga taaaatggcg ggccggcacg
      361 gcaataaagg ggtcgtctca cggattctac cggaagaaga tatgccctat cttccggatg
      421 gtactcctgt ggagattgtt ctaaatccgc ttggagttcc ctcgcgtatg aatgttgggc
      481 agatactgga gacccatctt ggatggtccg cgaaggcctt gggcatttat gtcacgagcc
      541 ctgtctttga tggcgctacg gagagagaga tcaaggacct tcttaaaaag gccggccttc
      601 cagagtcggg ccaggccaca ctttacgacg gtctcacagg tgaatcattc gatcggcctg
      661 tgaccgtcgg ccacatgtat atgatgaagc tgcatcacct ggtagacgac aaaatccatg
      721 cgcgctcaat cggtccatac tcgttggtga cgcaacagcc gttgggagga aaagcccagt
      781 tcggtggtca gcgattaggc gagatggagg tgtgggccct tcaggcttat ggagcggctt
      841 ctaccttaca ggaatttttg accgtcaagt ccgacgatgt gcccggccgg tccaggatct
      901 atgaggcgat tgtaaagggc gaaaactttt tagagcctgg cttaccggag tcctttaatg
      961 tgctggtaaa ggaactccag agccttgcgt tggatgtcga gctgattaag gcaaaagagt
     1021 agcataaaac ttattgcccc cctctccaaa cgggatggtg ggcgggataa ggaggattca
     1081 ttggaaggaa tctacagcct ttttgaaaaa ccgaaggatt ctgtttcgtt tgatgcgatt
     1141 cggataagga tcgcgtcccc ggaaaagatc cgatcctggt cttacggcga ggtcaagaaa
     1201 ccggagacga tcaattaccg ttcttttaaa cctgaacggg acgggttgtt ctgtgccaag
     1261 atttttggcc cgacgaaaga ctgggagtgc aattgcggaa agtacaaacg catgaagcat
     1321 cggggaatcg tttgtgacaa gtgcggtgtt gaggtgatcc aggccaaggt ccgccgagag
     1381 cggatggggc atatcgaact ggcagccccg gttgcccata tctggttctt gaaaggagtc
     1441 cccagccgga tcgggaatct tctggagatg agcctgaagc agttggaacg gatactctac
     1501 tttgaaagct acgtggtcac agatgccgga gagacctccc ttaaggaaaa ggaatcgctc
     1561 tcggaggaga aataccgaag ccttgtccag gaacacggcg atgccttcaa ggcagatatg
     1621 ggtgcagagg cgattcggga gttgctcaag aagatcaata ttgatgcgat ggcaaaagaa
     1681 ctgcacaaga agatcaaaga agccagctcg actgcgttga agaagaagca tacgaagcgc
     1741 cttcgggtcg tggaggcctt ccggaaatca ggaaaccggc cggagtggat gatcctggat
     1801 gtcatcccgg tccttccgcc ggaactccgg cctttagttc ctctcgatgg aggcagattc
     1861 gccacctcag acttaaatga tctctatcgc cg
//
LOCUS       DATMKH010000540         1102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3759543, whole genome shotgun sequence.
ACCESSION   DATMKH010000540 DATMKH010000000
VERSION     DATMKH010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3759543"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..>1102
                     /locus_tag="VNV63_06430"
     CDS             85..>1102
                     /locus_tag="VNV63_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62293.1"
                     /translation="MAHKIVKPYRAVVQSGIYEHTDSLHASGGMSGDDLAKIGYDAPE
                     SEKEGKSKNIKSVTLWEIYDLDHQVVTTVSPGCDYKLREVPLPDYLNGGFPFVQLAFT
                     PVPGEVYPMSEVAPHEGQIVELTKLLSIELNHLKRWNRQLLMLEGALAPEEMNKFKDG
                     NDGAIITAQPVAGKTLGDVIQPVPYAPIQSDIYGVWNQIFQVWQNIAGQDAASQGGQA
                     KTQTRTLGELRLSLQGGHARSDEKLDVLEDFIAEVGKKLLTIMQKKYDLPKLARIVGE
                     KTVKEKILKVLPERPSAQPTQPAPGGAPGQAPQPNPTASSSYSGDFGFSWTRQDILGD
                     MDVDV"
BASE COUNT          273 a          268 c          309 g          252 t
ORIGIN      
        1 tcggtatttg cctattatgt tcccttccgg gacatcgttt ttgatccgtc tgccacgtac
       61 ccagtcgcac ataacgcccg ctggatggca cataaaatcg tcaagcccta tcgggctgtg
      121 gtgcaatcag gcatctatga acacacggat tcgctccatg cttcgggggg tatgtccgga
      181 gacgatctcg ctaaaatcgg ctacgacgcg ccagaatccg aaaaagaagg gaaatcgaaa
      241 aatatcaagt cggtgaccct ctgggaaatt tacgatctgg accatcaggt ggtgaccacg
      301 gtgtctccgg gatgcgatta caaactccgg gaggttccgc tccccgatta tctgaacggg
      361 ggatttccct tcgtgcagtt agcttttaca ccggttccgg gagaagttta cccgatgtcc
      421 gaagtggctc cgcacgaagg tcagattgtc gaactcacaa aacttttgtc gattgaactg
      481 aaccacctaa agcgttggaa ccgtcagtta ctcatgctgg aaggggcgct cgctcctgaa
      541 gaaatgaaca aatttaagga tgggaatgac ggggcgatta ttaccgcgca accggtagcc
      601 ggaaaaacgt taggggatgt gattcagccg gtgccgtatg ctccgattca gtcggatatt
      661 tatggagtgt ggaaccagat ttttcaggtt tggcagaaca tcgcggggca ggacgcggcc
      721 tcccaggggg gtcaagcgaa gacccagaca cgcaccttgg gagaactccg actctctctc
      781 caagggggtc acgcccgaag tgatgagaaa ctggacgttt tagaggattt tatcgcggag
      841 gtaggtaaaa agttactcac aatcatgcag aagaagtacg atttacccaa attagcgcgt
      901 attgtaggcg aaaagacggt taaggaaaag atcttgaaag tgcttccgga aaggccttct
      961 gcacagccta cgcagcccgc tccgggaggt gctcccggtc aagcaccgca gccgaacccc
     1021 acagcttctt cctcttatag tggtgatttt gggttctcgt ggacccgtca ggacattttg
     1081 ggggacatgg atgtggatgt gg
//
LOCUS       DATMKH010000541         1490 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_571277,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000541 DATMKH010000000
VERSION     DATMKH010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1490
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571277"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            237..1481
                     /locus_tag="VNV63_06435"
     CDS             237..1481
                     /locus_tag="VNV63_06435"
                     /inference="COORDINATES: protein motif:HMM:TIGR01686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIC family phosphatase"
                     /protein_id="HXC62294.1"
                     /translation="MVEIRNVKLLNSERLDQLSPAANRLDVKSEIASGFPYKLAHAST
                     VADLMSRLIISAPPKKGIITDLDDTLWNGILGEVGAGNISWDIEHDSQPHGLYQRLLH
                     GLSEAGVLIAAASKNDPQIVGEALEREDLVLPRSALFPVDAHWGPKSESVGRILKTWN
                     IGADSVVFVDDSPMELAEVKTNYPDVECILFPKNDPQALIGLLYRLRDLFGKSALSEE
                     DAIRRESIRSSQKNGAGGVEDHGGNLDEFLAQADAEIALNFSKEPLDPRALELVNKTN
                     QFNLNARRYTDAAWDAYIRHRDTFLMVVAYKDKYGPLGKIAVVAGRSLGKTVFLDVWV
                     MSCRAFSRRIEDRCIHELFARFQAEKIVFDFKATPKNGPTGEFLARILGAKPEPQCSL
                     SKRQFLSPQTTTSHHVTEVPNG"
BASE COUNT          353 a          360 c          407 g          370 t
ORIGIN      
        1 gacttggcct ccgtagcttg ggtagttgga cgccttcaac gtttccggag attatcggga
       61 atgcgagggc cagattgtcc ctcttgacaa atctcgctga aatattatca aaggatattc
      121 cgttggtagc tagtttcccg actcttccgc tccttcccat ttcgttttca tcgggttggc
      181 aggcgagcgc attcgatctg gagcttcggg cttgcgtatc gtccacagct ttgaggatgg
      241 ttgagatcag aaacgtcaag ttactgaatt cggaacggtt ggatcagctc tctccagcag
      301 cgaatcggct ggacgtgaag tcggaaattg cttccggctt cccatacaag ctagcgcacg
      361 cttctacggt agccgatctg atgtcgcgtc taattatatc ggcgcctccg aagaaaggta
      421 taatcacgga cttggatgac actctttgga atggcatcct cggagaagtc ggcgccggca
      481 atatttcttg ggatattgaa catgatagcc agccccatgg tctttaccag aggctgcttc
      541 acggcctttc cgaagcagga gttttgattg ccgcagccag caagaatgat ccgcaaatag
      601 taggagaggc ccttgagcga gaggatctgg ttttaccgcg cagtgcccta tttcctgtgg
      661 atgcgcactg gggcccaaaa tccgagtcgg tcggccgaat tttaaagacc tggaatatcg
      721 gggccgattc ggtggtgttc gttgatgaca gtcccatgga acttgcggaa gtgaagacaa
      781 attatccaga tgtggaatgc attctgtttc ccaagaatga tccgcaagcc ctgattgggt
      841 tgttatatcg actgcgcgat ctcttcggga aaagtgctct ctccgaggaa gatgccattc
      901 ggcgcgaaag tattcgttca tctcagaaaa atggagccgg tggagtcgaa gaccatggcg
      961 ggaacctgga tgagtttctg gcacaagcgg atgcggaaat cgctctgaac ttttcaaaag
     1021 agccgctcga tcctcgcgcg ctcgagctcg taaataaaac gaaccaattt aatctcaatg
     1081 ccagaaggta taccgacgct gcctgggacg cctatatcag gcaccgggac acctttctca
     1141 tggtggtagc ttacaaggac aaatatggtc ctttgggcaa gatcgccgtc gtcgctggaa
     1201 gaagcttggg gaagacggtg tttttggatg tctgggtgat gagctgccgt gccttcagcc
     1261 ggcgaatcga agatcggtgc atccacgagc tttttgcgcg ttttcaggcc gagaagattg
     1321 tgtttgattt taaagcgact cccaaaaatg gaccgacagg agagtttctg gcacgcattc
     1381 tcggcgcgaa gcccgaaccg caatgctctc tttccaaaag gcaattttta tcgccacaga
     1441 caacaacctc ccatcatgta acggaggttc cgaatggata atacgcagga
//
LOCUS       DATMKH010000542         1096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4424523, whole genome shotgun sequence.
ACCESSION   DATMKH010000542 DATMKH010000000
VERSION     DATMKH010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4424523"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..>1096
                     /locus_tag="VNV63_06440"
     CDS             187..>1096
                     /locus_tag="VNV63_06440"
                     /inference="COORDINATES: protein motif:HMM:NF019313.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase fold protein"
                     /protein_id="HXC62295.1"
                     /translation="MAISIQAPWGGGKTSLMRMIQAQLDPKHPSLQEAKKSSVEIANY
                     ESSTRGQSRDGAVGTTAAKSAAGANVEEIKQELAGNQPPTPKLNEEIRKEKGRITVWF
                     NAWKYESTSQIWAGLADAIISQVGNRLSIVERERFFFRLHLKRLDVGKIRNRITNDLI
                     STFWSELVRLWWIYLVLPVATLLTYELGRSLFRLPWLSIAVGGNALIAAIHFVVTRFD
                     FDKKPAKLTLGEFLTVPDYDANLGFVHLVTEDLKKALELIPQEHPVVIFIDDLDRCSP
                     NKVADVIEAVNLFLAGEFENCMFVLGI"
BASE COUNT          271 a          272 c          299 g          254 t
ORIGIN      
        1 cagtcagatc ccgaaatcaa tcctgaggct caatctcccc cgtccggcga agctgttccg
       61 accggtgcaa gcggaccttc gactcatgta gcgcgggaca aatggaccac ggttgatgcg
      121 ctcggctatt acccatatgc atatgcgatc tatcgattcc ttacagatac agagacagct
      181 tccccattgg cgattagcat tcaggccccc tggggcggag gaaagacttc tctgatgaga
      241 atgatccagg ctcaactgga ccccaagcat ccgagtcttc aagaagcaaa aaagtcttca
      301 gttgaaatcg caaattatga gtcgagtacg cgaggccagt cacgggacgg cgcagttggt
      361 actacggcag caaagtctgc tgcaggagcg aatgtagagg aaataaagca ggaattggca
      421 ggaaaccagc ctccgacacc gaagctgaac gaggagatcc gaaaagaaaa aggcaggatc
      481 accgtttggt ttaacgcttg gaaatacgaa agcacaagcc agatatgggc tggccttgcc
      541 gacgcgatca tctctcaggt gggcaacaga ctgagtatag tggagcgaga acgcttcttt
      601 ttcaggttgc atctcaaacg tctcgatgtg ggcaagatcc gaaataggat cacgaacgat
      661 ctgatctcga cgttctggtc tgagcttgtc cgactttggt ggatatatct cgtacttccc
      721 gtcgctacgt tattgaccta tgagttgggt cgcagccttt tccggcttcc ttggctgtcg
      781 atcgccgttg gtggaaacgc cctgatagcg gccattcact tcgtggtaac gcgatttgat
      841 ttcgacaaga agccagcgaa actcactctg ggggagttcc tgacggttcc agactacgac
      901 gcaaatcttg gattcgttca tctggtgacg gaggatctga agaaagcgct ggagctgatt
      961 ccgcaggaac atccggtggt tatcttcatc gacgatctgg atcggtgttc ccctaacaag
     1021 gttgcggacg tcatcgaagc ggtcaatctt ttcctggcgg gtgaattcga gaactgcatg
     1081 tttgtcctcg gtatcg
//
LOCUS       DATMKH010000543         1783 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3126842, whole genome shotgun sequence.
ACCESSION   DATMKH010000543 DATMKH010000000
VERSION     DATMKH010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3126842"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            398..856
                     /locus_tag="VNV63_06445"
     CDS             398..856
                     /locus_tag="VNV63_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62296.1"
                     /translation="MASDDPIAQNSSRRIRMLGTCWVIYGIIRIIAAVWLVSFSNTAM
                     LMYGALLNRVPDPFTMMSFFHVFYTFLIALSVVCGALGVLAGLALLAGQRSGRTLALF
                     AGFLSLSDIPLGLTLGIYSLMVLLPAVSRYASPAVLPDPASGLRHHPSAT"
     gene            complement(931..1119)
                     /locus_tag="VNV63_06450"
     CDS             complement(931..1119)
                     /locus_tag="VNV63_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Thivi_2564 family membrane protein"
                     /protein_id="HXC62297.1"
                     /translation="MPLVQVVLVLIVIGLVLSLINRYIPMAGSIKSILNAVVVIAVIV
                     WLLNVFGLLTSFPRIHVG"
     gene            complement(1380..>1783)
                     /locus_tag="VNV63_06455"
     CDS             complement(1380..>1783)
                     /locus_tag="VNV63_06455"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXC62298.1"
                     /translation="VPSDTLRILLADDREAIRKRVRSILVSHGGFEVCAEASNGREAI
                     EKAQALKPDLIILDITMPITNGLDAARTIKRILPQTPIVILSVHKNRQIMKEAQGIGV
                     QGFVTKEEASRDLINAAEAVLHEQTFFPPEA"
BASE COUNT          401 a          478 c          465 g          439 t
ORIGIN      
        1 aaatcgagga gaaccaagtc cgggtgggat tccctggtcc tctgcacggc ttcctgtcca
       61 tcgattgcct ccgcacagac ttcccagctc gcatcctgct ctaccaaatg acgcagcacg
      121 ctccggacct ggggactgtc atcggcgatt aggattcgag tcatcaatgc cttccgattt
      181 cacagtgtca cagttagtgc caccctgtca attagcaaga tacctgagga gaaaatgccg
      241 gctctgtgaa aatttacact gaagattctc acgggacggt gtaaggccaa atccagaccc
      301 gaaatcaagg gtttctccta tgggcagctg gattcgagac agttaggatc aatgagcgtc
      361 gatcgtgggc taagtaaagg cacgaaggag agctcagatg gcttcggacg atccgattgc
      421 ccaaaactcg agccgccgaa tccggatgtt gggaacgtgc tgggtaatat atggaatcat
      481 ccggatcatc gccgcagttt ggcttgtgtc attctcgaat acggcgatgc tgatgtacgg
      541 cgccctgctg aatcgcgttc ccgacccgtt tacgatgatg agtttttttc acgttttcta
      601 cacgttcctc attgctctgt ctgtggtctg cggggcttta ggagtcctcg caggattggc
      661 cctgctcgcc ggtcagagat cgggacgcac gctggctctg ttcgcaggat ttctgtctct
      721 ctccgatatt ccgttagggt tgacgctcgg aatttacagt cttatggttt tactccccgc
      781 cgtctcgagg tacgcctccc cagctgttct tcctgatcca gcctccggtt taaggcacca
      841 tccttccgcc acgtagcagg agacgtaaca gaatggggaa gcgctcggct tccccattct
      901 caaacaatac ttcaaattca gttttgttgg ctatccgacg tggatccttg gaaaacttgt
      961 gagcagtccg aacacgttca agagccacac gatgacagcg atgaccacga cggcattcaa
     1021 aatagacttg atcgaacctg ccatcggtat gtaacggttg atcagagata ggacaaggcc
     1081 aatcacgatt agaaccagca cgacttgaac tagaggcatg atttattgtc ctctcattct
     1141 tcaaacgcgg ggttgggaat ccaagtggaa ggaccagacg taagtaagat tcataattcg
     1201 acgggagagg cagggtggac gccagcccag ccctctatat tttagcttcc ggtcctcctc
     1261 ccccgcgcct tcatcttcac cggacgcgct gccggggaca atacatgcga tatcgtacat
     1321 ggaccggaaa atcacagtag aaccacgtag agaacgaggc cactggtttg cacaaatgct
     1381 taggcttcag gtggaaaaaa ggtttgctca tgcagaacag cttccgcagc gttgatcaag
     1441 tcccggctcg cctcctcttt agttacaaat ccttggacac cgattccctg agcttctttc
     1501 atgatttgcc ggttcttgtg cacggagaga atcacgatcg gtgtttgcgg cagaattcgc
     1561 ttgatcgtcc tggcggcatc gagtccgttc gtgattggca tggtgatgtc caggatgatc
     1621 aagtccggct ttaacgcctg agccttttcg atggcttccc ttccgttgga agcttccgcg
     1681 cagacctcaa atccaccgtg ggagacgagg atggatcgca cgcgctttcg gatagcttcc
     1741 cgatcgtcgg caagtaggat tcgcaaggtg tcgctcggaa ctc
//
LOCUS       DATMKH010000544         1019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2156607, whole genome shotgun sequence.
ACCESSION   DATMKH010000544 DATMKH010000000
VERSION     DATMKH010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2156607"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..>1019)
                     /locus_tag="VNV63_06460"
     CDS             complement(82..>1019)
                     /locus_tag="VNV63_06460"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018560742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXC62299.1"
                     /translation="QVSEIGYGAWGIGKGMWIGAEDDESIKALNKAVDLGLNFIDTAL
                     VYGDGHSEKLVGQVVKARSERIYVASKVPPKNYQWPAQQGVPAREAFPADHVIASTEE
                     SLRNLGLETIDVQQFHVWSDEWADQGDWLEAIQKLKRSGKIRFFGVSINDNQPENAIR
                     LIETGAVDTVQVIHNIFEQAPEDRLYPACEQHQVGVIVRVPFDEGSLTGKITPDTNFD
                     SSDFRSRYFRDDRKQQVYDRVQKIISDVGIALDQMPDLALRYVLSSPAVSTVIPGMRS
                     IRNVEANCRAGDGKGLSKDQLNKLKTHRWERNFYG"
BASE COUNT          224 a          299 c          242 g          254 t
ORIGIN      
        1 aaaactgtca acgatgtttc cgaacgactg ttaacgatgt atccggtctg tacactcgag
       61 ctcgtcccta ccgctgaccg cctacccgta aaaattacgc tcccaacggt gagttttgag
      121 cttattaagt tggtctttgg acaatccttt gccatctcct gcccggcaat tggcttccac
      181 gttccgaatt gaacgcattc ctggaatcac ggttgaaacc gcgggggagc tcaggacata
      241 tcgcaaagcg aggtccggca tctgatccaa ggcgatgccg acatccgaaa taatcttttg
      301 tacccgatcg tagacttgtt gtttccggtc atctcgaaaa tagcggctgc ggaaatcgga
      361 agagtcaaaa ttggtatcgg gagtgatctt tccggtgagg ctgccctcat cgaacggtac
      421 tcgaacgatg acacccacct gatgctgttc gcaggccggg tatagacgat cttccggagc
      481 ctgctcgaaa atattgtgga tgacctgaac ggtatccact gcccccgttt caatcaatcg
      541 gatcgcgttc tccggctggt tgtcgttgat cgagacacca aagaaccgga tctttcccga
      601 tctcttgagc ttttgaatcg cttccagcca atcgccctgg tcagcccatt cgtccgacca
      661 aacgtgaaac tgttgaacgt caatggtttc caatcccaga tttcgtaagc tctcttcggt
      721 tgaggcgatg acatgatcgg ctgggaacgc ctctcgagcc ggtacccctt gctgagccgg
      781 ccactggtaa ttctttgggg gaaccttact agcgacgtaa atgcgttcgg agcgcgcctt
      841 caccacctgt cccacgagtt tctcgctgtg gccatccccg tacaccagag ccgtatcgat
      901 gaagtttagc ccaagatcga ccgccttatt cagcgctttg atcgattcat cgtcctcagc
      961 gccaatccac atcccctttc cgatccccca ggcgccgtac ccgatttcag aaacctgca
//
LOCUS       DATMKH010000545         1244 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2489544, whole genome shotgun sequence.
ACCESSION   DATMKH010000545 DATMKH010000000
VERSION     DATMKH010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1244
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2489544"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="VNV63_06465"
     CDS             complement(<1..692)
                     /locus_tag="VNV63_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62300.1"
                     /translation="MPRANHCFPAYMLLFLLLLPSGSWGQEVPERPTRLVIPDGTPVK
                     LQIVRTISSAHAQKGDHLGLVVTKDVMLGGFTIIQAGTIASGSVLGVKGKRPLGIGGK
                     VVIKVDSVKLTTGDEVALQACKEVKGSSRTKLIVAEMIITGLFYLPVTPVFLLSRGHD
                     STVLKGTEVTAHITGNSVVETAKLLGNADVSELHEMIDFLPSRTVDGEGREGDMLNLI
                     FIAKKEDLEGAF"
     gene            1029..>1244
                     /locus_tag="VNV63_06470"
     CDS             1029..>1244
                     /locus_tag="VNV63_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62301.1"
                     /translation="MRRRGINHLRLFSREVNYERFWGRFGCAQSLEVIDRDEKNNQQN
                     YDNWISSNGLLCLNGLSHARGQAQSRPP"
BASE COUNT          290 a          328 c          338 g          288 t
ORIGIN      
        1 tcgaacgccc cttccaaatc ttcttttttt gcgatgaaga tcaggttcag catgtcgccc
       61 tcgcggcctt caccgtcgac cgtgcgggaa ggtaggaaat cgatcatttc atggagttct
      121 gaaacgtcgg cgtttcccaa caacttcgct gtctccacga cagaattacc ggtaatgtgg
      181 gctgtcactt ctgtgccttt caggacagta ctgtcgtggc cgcgggaaag caggaacacc
      241 ggtgtgaccg gcaagtaaaa cagccccgta atgatcatct ctgccacaat aagcttggta
      301 cgggaacttc ctttgacttc tttgcaggct tgtaatgcaa cctcatcacc cgtagtgagc
      361 ttaactgaat cgaccttgat gactactttg cctccaattc cgagggggcg cttaccctta
      421 actccaagga ccgaaccgct ggcgatggtg cccgcctgga taatcgtgaa accccctagc
      481 atcacgtctt ttgtgaccac aaggccgagg tgatctcctt tttgagcgtg cgcagaggag
      541 atcgtccgga ctatttgaag tttgacagga gtaccatccg gaatgaccag ccgggtgggc
      601 ctttcgggta cttcctggcc ccaagaacca gaggggagga gaaggagaaa gaggagcatg
      661 taagcaggga aacagtgatt cgcgcggggc atgtggcgcc gccttccatt ggagtcaagc
      721 ctctcaatcg cgaaggctca agcagtagcc atcacgatgg gtcttcctct caccaagtat
      781 tggggatcct gggggcgttg tctggtcaaa attgcacaat cgcactgttc tccacgaaat
      841 tcctcggaag caatcactgg atcatgagca gccgtcaagg gcggaacgtc gcccccagag
      901 tagcggattc gcggtaatgt tgggattgga ggaccgcgtg gtcgaggaac caccccgtga
      961 cgatttcctc acgctccgac ttcagctaac ttttccatga tcctgctggc tcacaacgga
     1021 tcatgaaaat gcgccgacgt ggaatcaatc acttacggct attttcgagg gaggtgaatt
     1081 atgagcgatt ctgggggcgt tttggatgcg cccagagtct ggaagtcata gatcgtgacg
     1141 agaaaaataa tcaacagaat tacgacaact ggatttcatc gaacggctta ctgtgtctta
     1201 acggtttgtc tcacgccagg gggcaggctc aatcgcgccc gccc
//
LOCUS       DATMKH010000546         1086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_255836,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000546 DATMKH010000000
VERSION     DATMKH010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1086
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255836"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..878
                     /locus_tag="VNV63_06475"
     CDS             <1..878
                     /locus_tag="VNV63_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62302.1"
                     /translation="PGCFGSYVKPTEPPTILKNWTADEIFAVMNQRAQSIQQVKTLVT
                     VKIEGKRPPGFFLLSQGLQAALWAERPRPPAGRAGRIRLQGFNPVGGILFDLVSEDGN
                     LQLSVPGQSKDIQTALEEMLIRKGMKSSFSSELLDILASGGQPLIRHSEFSAIEQIGR
                     EIVLNQFRLSTDGKAHLIRKYWLDPDRLLTTQAVYFDPSGRPSVTAIYRDYQTIILPG
                     GAAILPGGVIPPGGASLSPDGASVSPGGGPESFWPREIMTTLNERTQLAITFSEVKLN
                     QPFQAGAFSLGISPQ"
     gene            878..>1086
                     /locus_tag="VNV63_06480"
     CDS             878..>1086
                     /locus_tag="VNV63_06480"
                     /inference="COORDINATES: protein motif:HMM:NF013012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine biosynthesis
                     protein TsaB"
                     /protein_id="HXC62303.1"
                     /translation="MVLGIETSTALGSVAIVEDQKLRGERRWKAEKGHAERLIEELDS
                     LLEKLSISMKALDGFAVTIGPGSFSG"
BASE COUNT          231 a          269 c          312 g          274 t
ORIGIN      
        1 tgcccggctg ctttgggagc tatgtcaagc cgaccgaacc gcctacgata ctcaagaact
       61 ggaccgctga tgagatcttt gccgtaatga accagcgggc tcaatcgatt cagcaggtca
      121 agacattggt cacggtcaaa atcgagggaa agcggcctcc cggttttttc cttctaagtc
      181 agggtcttca ggctgcgttg tgggcagaac ggccacgccc gcctgccgga cgggcaggcc
      241 ggatccggct tcagggtttt aatccggtgg gtggaatatt gtttgatctc gtctcagaag
      301 atggtaacct gcagttatcg gtgcctggcc agtcgaaaga cattcaaacc gctttggaag
      361 agatgctcat tcgaaaagga atgaagtctt ctttttcatc ggaattgctc gatatccttg
      421 cgagcggggg acaaccgttg atacggcact ctgagttttc agccatcgag cagatcgggc
      481 gggagatcgt tctgaatcag tttcgtttaa gtacggatgg aaaagctcat cttatccgca
      541 aatactggct cgatcctgat cgccttttaa ccacgcaagc ggtttatttc gatccctccg
      601 gccgtccatc ggttacagcg atttatcgcg actatcaaac tattattctt cccggagggg
      661 ccgctattct tcccggaggg gtcattcctc ccggcggggc ttctctatct cccgatgggg
      721 cttctgtatc tcccggcggg ggccctgaga gtttttggcc tcgggagatt atgacaactc
      781 tgaatgaacg gacccaactg gcaattacct tcagtgaggt gaagctgaat caaccttttc
      841 aggccggggc ttttagtctt gggatatcgc cgcaatgatg gtcttgggga ttgaaacctc
      901 taccgcactg ggctctgtgg ccatcgtgga ggatcagaag cttcggggag agcgccgttg
      961 gaaggcagaa aaagggcatg ccgagcggtt gatagaggag ctcgatagtc ttcttgaaaa
     1021 actttcgatc tcgatgaagg cgctggacgg ttttgcggtg acaatcgggc ccggttcctt
     1081 tagcgg
//
LOCUS       DATMKH010000547         3159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1847891, whole genome shotgun sequence.
ACCESSION   DATMKH010000547 DATMKH010000000
VERSION     DATMKH010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3159
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1847891"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="VNV63_06485"
     CDS             <1..544
                     /locus_tag="VNV63_06485"
                     /inference="COORDINATES: protein motif:HMM:NF013703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HXC62304.1"
                     /translation="VAYAAHQVGLAIEALSYLQGGAFSIAAATAVGKSVGAGKYKLAR
                     LKNWEANRIAVFIMACMGLIFFFFPYLLLRIFTQDPEVIRLGTLFLKIVAVMQVPLAI
                     TMVLEGSLKGAGDTRFLILVTVAGMWVVRVPLSSLFSYGLGLPLVFVWGVMVLDWFTR
                     MTILLFRYRSERWQEARVIR"
     gene            complement(545..1489)
                     /locus_tag="VNV63_06490"
     CDS             complement(545..1489)
                     /locus_tag="VNV63_06490"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HXC62305.1"
                     /translation="MDHTTSNPQTSITRPGESAEAQQNLDFLDEQTPLAELPKPSYRT
                     PIILFTLTVFTTLLAGATQQGVNPFREPLSLLKGIPFSFTLLAILLTHEFGHYFTSRY
                     HKVPATLPYFIPAPPPFIAGTFGAFIRMTAPILRKNAIFDIGISGPIAGFVVAVVAVA
                     VGLDFSTIVKTDSAEGLKLGSPLIFSWLSHLILGNPGEGHDILLHPIAFAGWIGLFVT
                     SLNLIPMGQLDGGHVVYALFGEKQWFISLAMIPILLWLGYVGWSGWFVWAFLPMIFGL
                     RHPPVLDKDKPLDRRRIIVGWLGLIMFVITFIPVPFSI"
     gene            complement(1514..1690)
                     /locus_tag="VNV63_06495"
     CDS             complement(1514..1690)
                     /locus_tag="VNV63_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62306.1"
                     /translation="MKDALESLIGQKVEAIYEGIVYRGILVGANDDEIFLQTVMEWIS
                     LPLEGIASVKKAEV"
     gene            1825..2067
                     /locus_tag="VNV63_06500"
     CDS             1825..2067
                     /locus_tag="VNV63_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HXC62307.1"
                     /translation="MPQNLQVDQRLDCKGLACPLPVIKTKQTIDKMVVGQVLEMISTD
                     PGSTPDMQAWSRKTGHELLEAKQEGNLYIFYIRKTK"
     gene            2086..2706
                     /locus_tag="VNV63_06505"
     CDS             2086..2706
                     /locus_tag="VNV63_06505"
                     /inference="COORDINATES: protein motif:HMM:NF025066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HXC62308.1"
                     /translation="MSSIVDNKELGELIEERVKTAVDQKVNAEVEKRLNELIEAKLKA
                     VKEKETKPKRLAIAVVSKGTIDAAYPALVLATASVALDMECGVYFSFFGLNILKKANQ
                     DGLKIVPLGNPAMPIPLPNLLAILPGVSAFATWFMKRQIKQKKIASIPELMAIAKESG
                     VKLWPCQMAMDMFNIKREDLIDGLEEPVGAATFLEFAADADITLFI"
     gene            2719..3147
                     /locus_tag="VNV63_06510"
     CDS             2719..3147
                     /locus_tag="VNV63_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62309.1"
                     /translation="MPPEKPKDKFVIFAHSGTYDRLHQVATLALTAAAMGQEVYIILF
                     FFALKKFCSGELDKPDFPQEYQAFGKQVSKLMEEKKVPKVSEMLRDAKGIGRVKIIIC
                     SAGLEYMDIEKGEQSDLVDEVWGLPTVLNEVKGASTVLYI"
BASE COUNT          786 a          743 c          879 g          751 t
ORIGIN      
        1 ggtggcttac gccgctcacc aggtgggtct ggctattgag gcgctctcgt atcttcaagg
       61 gggcgccttt tcgattgcgg cagcgactgc ggtgggcaag agtgtgggag ctggtaagta
      121 caagcttgca aggctcaaga attgggaggc caatcggatc gccgtcttca tcatggcctg
      181 tatgggactg atctttttct tcttccccta cctgcttctc cgtatattta cacaagaccc
      241 ggaggtaatc cgtctcggca cacttttcct caaaatcgtt gccgtgatgc aggtgccgct
      301 tgcgatcacg atggttcttg aaggctctct gaaaggcgcc ggcgacaccc gttttctcat
      361 actggtgacg gtcgcgggga tgtgggtggt gagggttccg ctctcctctc ttttctctta
      421 cggtctgggc ctccccctgg tctttgtctg gggcgtgatg gtgctcgact ggtttacacg
      481 tatgacaatt ctgctgttca gataccggtc tgagcgctgg caagaagcga gagtaatacg
      541 gtgattagat agaaaagggg accgggataa aggtgatgac aaacatgatc aatcccagcc
      601 agcccacaat gatgcgtctg cggtcgaggg gtttgtcttt gtccagaacg ggcggatggc
      661 ggagtccaaa gatcatcggg agaaacgccc acacaaacca gcccgaccat ccgacatagc
      721 ccagccataa aaggattgga atcattgcaa gcgagatgaa ccactgtttt tcaccaaaca
      781 gggcgtagac gacatgacca ccatccagct ggcccatcgg gatcaggttc agcgaagtca
      841 caaagagacc gatccaccct gcaaaggcga ttggatggag gaggatatcg tgcccttcgc
      901 ctggattccc gagaatcaaa tgggacagcc atgaaaagat caggggggaa cccagcttga
      961 gcccttccgc cgaatctgtt ttgacaatcg ttgagaaatc taatccgacc gcgaccgcaa
     1021 cgaccgccac gacaaatccg gcgatcggtc ccgagatccc gatgtcgaat atggcattct
     1081 tcctaaggat tggcgcggtc atccgaatga aggctccgaa cgtaccggca atgaatgggg
     1141 gaggggccgg aatgaaataa ggcagtgtgg ccggcacctt atgatatcgg gaggtgaaat
     1201 aatgtccgaa ttcgtgtgtc agtagaatag cgagcagcgt gaaggaaaat ggtattcctt
     1261 tgaggagcga tagaggttcc cgaaacggat ttacaccttg ctgggtcgct cccgctaata
     1321 gggtggtaaa gaccgtgagg gtaaacagga taatcggcgt cctgtacgat ggtttgggaa
     1381 gttcagccaa cggtgtctgt tcatcgagaa aatcaaggtt ctgctgggct tcagccgatt
     1441 caccggggcg ggtgattgag gtttggggat ttgatgtcgt gtggtccatg atttctatcc
     1501 aagtctcgga atatcagacc tctgctttct tgacggatgc gattccctca aggggaagcg
     1561 atatccattc catcacggtc tggaggaaga tctcatcatc gttcgcgccc accagtatgc
     1621 ctcgataaac gatgccctcg taaatcgcct ccaccttctg gcctattaaa ctttccaatg
     1681 catctttcat tactttaaca gtctgctatc ctttgatgtt attatatcat atcgacccga
     1741 aatttggatt gcaactggaa accctgcact tgacttctga gacctatgag gcttatcata
     1801 aatctatatc gagggagaac acaaatgcct caaaatcttc aggttgatca gcggctggac
     1861 tgcaaggggc ttgcctgtcc gctgccggtg atcaagacga agcagacgat tgacaaaatg
     1921 gtggtgggcc aggtattgga aatgatctca accgatccgg gttcgacccc ggacatgcag
     1981 gcctggtcga gaaagaccgg tcatgagcta ctggaggcaa agcaggaagg caatctatat
     2041 attttttata ttcgaaagac aaagtaggtc gaggaaggag tgaaaatgtc ttcaatcgtc
     2101 gacaacaaag aattagggga gttgatcgaa gagcgagtca agacagcggt agatcaaaag
     2161 gtcaatgccg aggttgagaa gcgcttgaac gaattgatcg aggccaaact caaggcggtc
     2221 aaggaaaagg aaaccaaacc gaagcgttta gcgattgcgg ttgtctctaa aggtacgatt
     2281 gacgccgctt atcctgcact ggtcttggcc acggcgtcag tagcgttgga catggaatgc
     2341 ggtgtctact tctccttttt cgggttgaac attctcaaga aagcgaatca agatggctta
     2401 aaaattgtgc cgttgggcaa tcctgcaatg ccgattccgc ttcccaatct cctggcgatc
     2461 cttccgggtg tttcggcttt tgcgacttgg tttatgaaac ggcagatcaa gcagaaaaag
     2521 attgcgtcca ttcctgaact catggcgatt gccaaggaga gcggggttaa gctttggcca
     2581 tgtcagatgg cgatggatat gttcaacatt aagcgcgaag atctgattga cgggcttgaa
     2641 gagccggtcg gggctgctac tttcctcgag tttgccgcag acgccgacat cacactcttt
     2701 atctagctga tcttgaaaat gcccccggaa aaacccaaag acaagtttgt aatctttgcc
     2761 cacagcggaa cctatgatcg gcttcatcaa gtggcgaccc tggcactgac cgcagccgcg
     2821 atgggccaag aggtttatat catcctgttc ttctttgctt tgaaaaagtt ctgctccgga
     2881 gagttggata agcctgactt cccgcaggag tatcaggctt tcggaaagca ggtttcaaag
     2941 ctcatggaag agaaaaaggt gcccaaggtg agcgagatgt tgagggacgc caaaggcatc
     3001 ggccgcgtca agatcatcat ctgcagcgcc ggtctcgagt acatggatat cgaaaaaggc
     3061 gagcagtcgg atcttgtgga tgaggtctgg ggacttccta cagttttgaa cgaggtgaag
     3121 ggcgcatcca cggtcttgta tatttgagac tgtcgcaaa
//
LOCUS       DATMKH010000548         1225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4428225, whole genome shotgun sequence.
ACCESSION   DATMKH010000548 DATMKH010000000
VERSION     DATMKH010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4428225"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VNV63_06515"
     CDS             complement(<1..188)
                     /locus_tag="VNV63_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HXC62310.1"
                     /translation="MAKETKIAFDSPSKAGALPENLIFGNAPSMQLVRRIVERAASSF
                     LPVLLCGESGVGKEIMAR"
     gene            complement(612..>1225)
                     /locus_tag="VNV63_06520"
     CDS             complement(612..>1225)
                     /locus_tag="VNV63_06520"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXC62311.1"
                     /translation="TLEAPHRMLFEKSLEKMNLSAHDHILEVGCGGGWASHAMTERVA
                     PDSRVVGIDISDGMIRRARARFTKVHNLNFICAGAESMPLSGASFTKIFCLEAFFFFE
                     DQEKVLREMLRVATPGGEVFLIFGLHKDYPEGLNAVEEIRLPVHIRSSAEYQDMLRRT
                     GWSEVRAETFIRRREPGEKTDVHDRALFLYGRKPAVAKRAAGV"
BASE COUNT          287 a          329 c          310 g          299 t
ORIGIN      
        1 tcccgcgcca tgatttcttt acccactccg ctctcgccgc acagcagcac tgggagaaac
       61 gaggaggccg ctcgctccac aatccgccgg acaagctgca tcgagggggc attaccaaat
      121 atcaggttct ccgggagagc ccctgccttg gagggcgaat caaatgcgat cttggtttcc
      181 tttgccattc ctgaagtcac agcgcctttg ggggctcccc ccttgaccgt tattcgaatg
      241 gggggccaaa ccttgaagtc gtttgtcttg catgaaagat gcacatgagc ggccaagcac
      301 agtggcatca aatttggcag cgtagattta tgtaagtgct ttacttacag agagatagtc
      361 catcttgagt gaatgaagag gcgcggtttt gagccgctgt gtggggaata gtctttcaca
      421 tcaaggaaaa gtgtttccct atagagataa gtcattagtg ctaattctca tcgccttagg
      481 agcccccaaa ttcaggtgcg gccaccggca tgtgctaaat ccgggggcat agagaagcga
      541 aatccgacca acaatttccc aaactttcaa atcacagact ctcggggggc tcagcaatat
      601 tgatttggag ctcagactcc tgccgctctt ttggcgactg ctggttttcg gccgtaaaga
      661 aacagtgccc ggtcgtggac atcagtcttt tcgcctggtt cgcggcgccg aatgaaggtt
      721 tccgcgcgaa cctcggacca cccggttctt cgcagcatat cctgatactc ggcggaactt
      781 cggatatgca caggtaaacg gatttcttct accgcgttca atccctcggg ataatcttta
      841 tgcaacccga aaatcaggaa aacttcgcca cccggggtcg cgactctgag catctcccgg
      901 aggacctttt cctgatcttc gaagaaaaag aaagcctcca ggcagaaaat ttttgtaaag
      961 gaggcgccag aaagtggcat gctttccgct ccggcacaga tgaagttcag gttgtggacc
     1021 tttgtaaatc tcgcccttgc acggcggatc attccatccg agatgtcgat ccctacaacc
     1081 cttgaatctg gtgcgactcg ttcggtcatt gcgtgagaag cccatccccc gccgcaacct
     1141 acttctaaaa tatggtcgtg cgcggaaagg ttcatctttt caagggattt ctcaaataac
     1201 atccggtgtg gcgcttccaa ggttt
//
LOCUS       DATMKH010000549         2286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4719153, whole genome shotgun sequence.
ACCESSION   DATMKH010000549 DATMKH010000000
VERSION     DATMKH010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4719153"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="VNV63_06525"
     CDS             complement(<1..382)
                     /locus_tag="VNV63_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020675756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTAP family purine nucleoside phosphorylase"
                     /protein_id="HXC62312.1"
                     /translation="MKRARKVSSKPKAEIGIIGGSGLYQMEGLSRLHEVRIKTPFGPP
                     SDAMMIGAIDGIPVAFLSRHGRGHRISPTDINFRANIFAMKLLGVRRILSVSAVGSMK
                     EKIHPGHLVIPDQFVDLTKRRISTF"
     gene            complement(407..688)
                     /gene="hfq"
                     /locus_tag="VNV63_06530"
     CDS             complement(407..688)
                     /gene="hfq"
                     /locus_tag="VNV63_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011455365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HXC62313.1"
                     /translation="MFKNNSNLQDVFLNHPRKEKIPVTVHLMTGVKLAGVIKGFDSFA
                     ILLKHTTQELIYKHAISVIQPQKEVSRFLDLQWQMAEREDQKPQKAEIS"
     gene            complement(675..1730)
                     /gene="miaA"
                     /locus_tag="VNV63_06535"
     CDS             complement(675..1730)
                     /gene="miaA"
                     /locus_tag="VNV63_06535"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HXC62314.1"
                     /translation="MSPYNNKKLLFLVGATAVGKTAVALELAERLGSEIINADSRQVY
                     RGMDIGTSRPTPAEQTRVRHHLLDLVNPDERFSAGIYKRLAEEVIEQQRREGRLPFIV
                     GGTGLYLKALAYGLWAGPHADWRIRSRWLEEQKRHGEGYLHQQLYQIDPVSAARIHPR
                     DSSKIIRALEVFELEGKPLSYFHEQHRFQDRRWKVVWIGLRRSRQDLYPRIEQRVDQM
                     LAAGMIEEVKRLLANGYSEQTPAMLGLGYRHLQGYLHGAIPWEVALQQWKRDTKRFAK
                     RQETWFKAEPAIHWFDLEKNEPVGKTADRILEWLAMQLTHITCVDLVSAPASRVPGER
                     EGEASPYSSRGGICVQE"
     gene            complement(1798..>2286)
                     /locus_tag="VNV63_06540"
     CDS             complement(1798..>2286)
                     /locus_tag="VNV63_06540"
                     /inference="COORDINATES: protein motif:HMM:NF020260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutL"
                     /protein_id="HXC62315.1"
                     /translation="ILYERLLSRMARGDSSGGAETQPLLIPETLELSARDAVLLRSHL
                     NELKETGLEIEEFGSNSFVIRSVPTIVARKDLKRLVLDLLDDFREWDRTAPMEETKKR
                     LVASLACHSAIRAHDSMTLPEMSALLRDLQDYPAVHSCPHGRPIQRRFTLNDLEKMFG
                     RR"
BASE COUNT          495 a          699 c          561 g          531 t
ORIGIN      
        1 aaaaagtgct gatccgccgt ttcgtgagat ccacaaactg gtccgggatc acgagatgac
       61 cgggatggat cttctccttc atgctcccca ccgcactcac agacaggatc cgtcgcaccc
      121 ccaggagttt catcgcaaag atgttcgcgc ggaaattgat atcggtcgga gaaatccgat
      181 gcccacggcc gtgacggctc aggaaggcca ccgggatgcc gtctatcgcc ccgatcatca
      241 tcgcatcgct ggggggtcca aagggcgtct tgattcggac ttcgtgaagc ctgctcagcc
      301 cttccatctg gtacaggccg ctgccgccga taatgccgat ctcggccttg ggtttactgg
      361 ataccttccg ggctcgcttc atcttttttc ccccaaaatc tcattgctaa gagatctccg
      421 ccttctgagg cttctgatcc tcgcgttccg ccatctgcca ctgaagatcc agaaaccggc
      481 tgacctcctt ttgcggctga atgacgctga tcgcatgctt atagattagc tcctgtgtgg
      541 tgtgctttaa gaggatcgca aaactgtcaa atcccttgat gacccctgcc agtttaacac
      601 cggtcataag atgaaccgtt accggaatct tttctttgcg gggatggttg agaaacacgt
      661 cctgaagatt gctgttattc ttgaacacag atccctcctc tactactata ggggctcgcc
      721 tccccctctc gctcgccggg tacccgtgaa gcgggtgcgc tgaccagatc cacacaggtg
      781 atgtgagtca gctgcatcgc caaccattcc aaaatacgat ccgcagtctt acccaccggt
      841 tcatttttct caagatcgaa ccaatgaatg gccggttcgg ctttaaacca ggtctcctga
      901 cgcttcgcaa accgcttcgt gtcccgcttc cattgttgaa gcgcaacttc ccacggaatc
      961 gcaccgtgca ggtatccttg aagatggcga tagccgagcc caagcatcgc aggggtctgc
     1021 tcggaatacc cgttggccag gagcctcttc acctcttcga tcattccggc ggccaacatc
     1081 tgatcaaccc gttgttcgat cctgggatag aggtcctgcc ggcttcgcct cagtccgatc
     1141 cagaccacct tccatcgccg gtcctggaag cgatgctgct catgaaaata agaaagaggc
     1201 ttcccctcca gttcaaacac ctcaagggca cggatgatct tggacgagtc ccgcgggtga
     1261 atcctggccg cggagacagg atcaatctga tagagctgct gatgcagata gccttcgcca
     1321 tgccgttttt gctcttccag ccaacgtgaa cggattctcc aatccgcatg ggggcccgcc
     1381 cacagcccat acgccaaggc cttcaggtag agaccggtcc cacccacgat gaacggcagc
     1441 cggccttcac gcctttgctg ttcgattact tcttcagcca gacgtttgta gatcccggca
     1501 ctaaaccttt catccggatt caccagatcc aagaggtgat gacgtacacg cgtctgttcg
     1561 gcaggggtcg gcctggaggt tccgatatcc attccccgat aaacctgacg ggaatcggca
     1621 ttgatgatct cgcttcccaa tcgctcggcc aactccagtg ccacagcggt cttccccacc
     1681 gcggtagccc cgacaagaaa aagaagtttt ttattattat atgggctcac gtcgccccct
     1741 ccaaatggca aagcctttgg agcctcccct tctcgctctc tttgctcgct gactcactta
     1801 tcttctcccg aacatctttt cgagatcatt cagcgtaaag cggcgttgga tcggccttcc
     1861 gtggggacag gaatgtaccg cgggataatc ctgaagatcg cggagcaggg ccgacatctc
     1921 aggaagcgtc attgaatcat gggcccggat cgcgctatga caagccagag atgccacaag
     1981 ccgctttttg gtttcttcca ttggtgcggt gcggtcccac tccctaaagt catccaggag
     2041 atctaacacc aaccgcttaa gatctttccg ggccacaatc gtcgggacgg aacgaattac
     2101 aaaagaattc gagccgaact cttctatctc caggccggtc tccttcagtt cattaagatg
     2161 cgaccgaagc agaaccgcgt cgcgcgcaga gagctccagg gtttcaggga tcagtaacgg
     2221 ctgcgtttcc gcgccaccgc ttgaatcacc acgggccatt cgagataaca atcgctcgta
     2281 cagtat
//
LOCUS       DATMKH010000550         1481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1217402, whole genome shotgun sequence.
ACCESSION   DATMKH010000550 DATMKH010000000
VERSION     DATMKH010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1481
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1217402"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..511
                     /gene="mutT"
                     /locus_tag="VNV63_06545"
     CDS             110..511
                     /gene="mutT"
                     /locus_tag="VNV63_06545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HXC62316.1"
                     /translation="MKSKESSIQVAVALIQHDGRYLIAQRLNASPLGGYWEFPGGKCM
                     PGESYEDCLRREMREELGVEVEVLGLYRVIEHAYPDRTVLLYFYRCRIMSGEPQALGS
                     QEIRWAKREELGQYRFPPANEKLIQELMSEI"
     gene            516..>1481
                     /gene="thiI"
                     /locus_tag="VNV63_06550"
     CDS             516..>1481
                     /gene="thiI"
                     /locus_tag="VNV63_06550"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HXC62317.1"
                     /translation="MKAVVVHYHEIALKGRNRPRFTLKLVRNLQTACKGLSISQVKSL
                     PGRVRMQLNDEKDWPELRSRIERVFGISNFSCSQSTSREMETLKTAILESLGSVRCAS
                     FRVLTRRADKTFSLTSQEINREIGAAVQQRYGFPVNLENPELTIYIELLPEEAFFYTE
                     KFAGPGGLPVGISGRVAALISGGIDSPVAAHRMMKRGCQVVFVHFHSYPYLNKTSQEK
                     VTELVQHLTRYQYASQLYLVPFGEMQREVVLGAPAPLRVVLYRRLMMRIAQELAGWAK
                     AKALVTGESLGQVASQTLENLLVIEEASEIPILRPLIGMDKNEIIH"
BASE COUNT          342 a          361 c          417 g          361 t
ORIGIN      
        1 caccaattta cccccttcgt ggatttcgat tttagtgggg acacaagtgt tagtcaagag
       61 tcgtcatttt atttcggttt gacttcatat ataatatggc ctttcaatga tgaagagcaa
      121 ggagtcctcc attcaggttg cggttgccct gatccaacat gatggccggt atctgatcgc
      181 tcaacgcctc aacgcgtcgc ctctcggcgg ttactgggaa ttccccggtg ggaagtgtat
      241 gcccggggaa tcgtacgagg actgcctgag acgtgagatg agagaagagt tgggcgtgga
      301 ggtagaggtc ctgggactct accgtgtaat cgagcatgcc tatcctgatc gaaccgtgct
      361 gctttatttt tatcgctgcc gtatcatgtc cggcgagcca caggctttag gcagccagga
      421 gatccgatgg gccaagcgcg aagagctggg acagtatcgc tttccacccg ccaatgagaa
      481 gctgatccaa gaactgatgt cagagatatg aaaacatgaa agctgtagtc gtccattatc
      541 atgagatcgc cctaaagggc aggaaccggc cacgcttcac tttaaaattg gtccgcaatc
      601 ttcagacggc ctgtaaaggc ctttcgatct cgcaagtcaa aagccttccc ggtcgtgtac
      661 gcatgcagtt aaacgatgag aaagactggc ccgaattacg aagccggatc gaacgagtct
      721 ttggaatctc caatttttct tgttcccaat ccacatcgag ggagatggaa acattaaaga
      781 cggctattct agaaagtctg ggatcggtcc ggtgtgcatc cttccgggtc ttgacccgcc
      841 gggcggacaa gaccttttca ctcacttctc aagagatcaa tcgtgaaatt ggtgcagcgg
      901 tccagcagcg gtacggcttt ccggtcaatt tagagaatcc ggaactcacg atctatatcg
      961 agctgttgcc ggaagaggcc ttcttttata cggagaagtt tgcggggccg ggcggcctgc
     1021 cggtcgggat cagcggccgc gtggccgcat tgatctcagg cggtattgac tcgcctgtgg
     1081 cggctcatag aatgatgaaa cgcggctgcc aggtggtgtt tgtgcatttt cacagctatc
     1141 cttatctgaa taaaacctct caggagaagg tgaccgagct cgttcaacac ctgacccgtt
     1201 atcagtacgc ctcccagctc tatcttgtcc cgtttggtga aatgcagcgg gaagtggtgc
     1261 tgggtgcccc cgcgcctctg cgcgtggtgt tgtaccgtcg gctgatgatg cggatcgcgc
     1321 aggagcttgc aggatgggca aaggcaaagg cactggtcac cggcgaaagc ctgggtcagg
     1381 tggcttctca gacactcgag aatcttttgg tgattgaaga ggcatcggag attccgatct
     1441 tgagaccttt gatcggaatg gataaaaatg agattataca c
//
LOCUS       DATMKH010000551         1944 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3765410, whole genome shotgun sequence.
ACCESSION   DATMKH010000551 DATMKH010000000
VERSION     DATMKH010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3765410"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..228)
                     /locus_tag="VNV63_06555"
     CDS             complement(31..228)
                     /locus_tag="VNV63_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like protein Pup"
                     /protein_id="HXC62318.1"
                     /translation="MSKQERKERKREPGKKEGESGAQPKVQEKGKKIKEDLDKLIDEV
                     DDVLEKNAEEFVKNYVQKGGE"
     gene            complement(243..1724)
                     /gene="dop"
                     /locus_tag="VNV63_06560"
     CDS             complement(243..1724)
                     /gene="dop"
                     /locus_tag="VNV63_06560"
                     /EC_number="3.5.1.119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="depupylase/deamidase Dop"
                     /protein_id="HXC62319.1"
                     /translation="MSHPRVLGTETEFGIATKDPESVDPVSNSLLLISNYPALPSARI
                     LWDYENENPLLDLRGFEVEGERERPGPEYNKLLNKLLANGGRLYVDGAHPEYSTPECT
                     NARDVVMFEKAGERILDLCRQLANRVKEEDKFFIYKNNTDGKGNSYGYHENYLMPRAL
                     SFDRIMQPLIPFLVTRQIYAGAGKVGAENRMPPISYQLSQRSDFFESLIDLNTMVKRP
                     IINTRDEPHADPAKYRRLHVIIGDANMSEFTTYLKVGTMSIVLSMIADAYEIKGIELE
                     DPVKTMKEVSRDQKLKQTLRLTDGRTITAIEIQRIYLEHAHRYLQEQPPDPIAQDILV
                     KWGAVLDQLAKDPMQLSREIDWVIKKELIESYMAKKGCGWEDSRISMMDLQYHDVNLQ
                     KGLYYALERGGYVERIVSYDEIARAETMPPLDTRAYFRAMCLKKYPKEVYAASWSSVL
                     FDVGNTTIKRVPLMEPLRGTETLTKGLLEESNSVEELLARIKA"
     gene            complement(1738..>1944)
                     /locus_tag="VNV63_06565"
     CDS             complement(1738..>1944)
                     /locus_tag="VNV63_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009358583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome ATPase"
                     /protein_id="HXC62320.1"
                     /translation="QKGLIAEDLLRAVKDEYKEHEDLPNTTNPDDWAKIAGRKGEKII
                     HIRTIAGRATEPRNIETVSTGHYL"
BASE COUNT          430 a          546 c          440 g          528 t
ORIGIN      
        1 aagaaaaagg gcctgtttta aattccacgg ttactctccg cctttttgga cgtaattctt
       61 cacgaattct tctgcgtttt tctccagaac gtcgtcaacc tcatcaatca atttatccag
      121 gtcctctttt atttttttgc ccttctcttg aacctttggt tgggcaccag actccccttc
      181 cttctttccg ggttctcgct tacgttcttt tctctcttgc ttagacactg gctctcaccc
      241 ccttatgctt taatgcgggc caaaagctct tccaccgagt tggattcttc caagaggccc
      301 ttggttaagg tttctgttcc gcggagcggt tccataagcg ggactctctt gatcgtggta
      361 ttcccaacgt caaacagaac cgagctccag cttgcagcat aaacttcttt gggatacttc
      421 tttaaacaca tcgcacgaaa ataggcccgt gtatccagcg gcggcatcgt ttctgccctg
      481 gcgatctcgt catagctgac gatccgctcc acataacctc cgcgctccaa ggcataatat
      541 aatcccttct gcaggttgac atcatggtac tgtaaatcca tcatcgaaat ccgggagtcc
      601 tcccatccac agcctttctt ggccatataa gattcgatca gctctttctt gatcacccag
      661 tcaatctcgc gagaaagctg catcggatct ttcgccaact ggtcgagaac cgcaccccat
      721 ttcaccaaaa tatcctgagc aatcggatcc gggggttgct cctgcagata acgatgggcg
      781 tgttccaggt agatacgttg aatctcaatc gccgtgatcg ttctgccatc cgtcaaccgg
      841 agggtctgtt ttaacttctg gtccctcgag acctctttca tcgtcttaac cggatcttct
      901 agctcaatcc ccttaatctc ataggcatcc gcgatcatgg acaacacaat cgacatcgtc
      961 cccactttca gataagtggt gaactccgac atattggcat cgccgataat gacatgcagg
     1021 cgacgatact tggccggatc ggcatgaggt tcgtcccgcg tgttgatgat cggccgcttg
     1081 accatggtat tcagatcgat cagggattca aaaaaatcgg agcgctgaga aagctgatag
     1141 gagatcggcg gcatccgatt ctccgccccg accttgccgg caccggcgta gatctgccgg
     1201 gtcaccaaga acggaatcag gggctgcata atccgatcaa aggacaaggc acgcggcatc
     1261 agatagtttt catggtaacc atagctgttt cctttcccat cggtgttgtt cttataaatg
     1321 aaaaatttgt cttcttcttt gacccggttg gctaattgac gacagagatc cagaatacgc
     1381 tcgccggcct tctcaaacat cacgacatcg cgggcattag tgcattccgg cgtagagtat
     1441 tccgggtggg ccccatccac gtacaaccgc ccgccattgg ccagaagttt attgagaagc
     1501 ttgttgtact ccggacccgg tcgttcgcgc tcgccttcta cttcaaaccc tcgaaggtct
     1561 agaagcggat tctcattttc gtagtcccac aagatccttg cggatggaag ggcaggatag
     1621 ttactgatga ggagaagcga attggaaacc ggatcaacag actctggatc cttcgtggca
     1681 atgccaaatt ctgtttctgt tccgaggacc cgtggatgac tcattacacc cccgtcgtta
     1741 gagatagtga ccggtcgaga ccgtctcaat atttcgaggc tcggtagccc tcccagctat
     1801 cgtgcggata tggatgatct tctcgccctt gcggcctgca atcttggccc agtcatcggg
     1861 attggtcgta ttcggaaggt cttcatgctc cttatactca tccttgaccg cacgcaagag
     1921 gtcttcagcg atcagacctt tctg
//
LOCUS       DATMKH010000552         1089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_261252,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000552 DATMKH010000000
VERSION     DATMKH010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261252"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..>1089
                     /locus_tag="VNV63_06570"
     CDS             154..>1089
                     /locus_tag="VNV63_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62321.1"
                     /translation="MTLRKIASLLLLALDVVIPKGAESKPFRKVLILSGGELRFSAGL
                     AMLATLYQRGWVPDVTIYTCGFSKIPGAYLNSLTQLQPGQPDPAKWYEAALSDEYFRI
                     MQRVKVNKDFFSRVVDKDGNTASSLLYGQMTSRIAGMRLKKGVNNASELPFLSSLLRT
                     NPNPFRMTPVAIYPALYRDNKDTLLDVPFDLSPPGMNIPFSANKMRAIFVATKILYPR
                     ELQDKPRPENSQPLYQEVVFTDRLTADEIARVKYEKSPIAKMFPKAPIHTDVEVRSGY
                     RVWDVARAALADMYLTAPMWLEDPTGGHPKELFITG"
BASE COUNT          265 a          310 c          263 g          251 t
ORIGIN      
        1 gcgagcgtta cccacgtgaa gggggccgat tggggtttgc gatgcctttc aatctttcaa
       61 tccaggacct gcaaatgcta atgcttacga tctgaggcgg tgcattgtca gcatatactc
      121 tccctccact tataagacca ggaggtcttg tcggtgacac tacgaaaaat tgcttcgctc
      181 ctacttttag ctttggacgt cgttattcct aaaggggccg aatcgaaacc ctttcgcaag
      241 gtgctcattc tttcaggagg cgagcttcgc ttctccgcgg gcctcgcgat gctggccaca
      301 ctttatcaga ggggatgggt tcccgatgtg accatctaca cctgcggatt ctccaagatc
      361 ccgggcgcgt acttaaactc actcacccaa ctccagcccg gtcaacccga tccggcaaaa
      421 tggtatgagg ccgctttgtc ggacgagtac ttccgaatca tgcaaagagt caaagtcaat
      481 aaggatttct tttctagagt cgtggataaa gacggaaaca ccgcttcgag cctgctttac
      541 gggcaaatga cttcccggat cgcgggaatg cggttgaaga aaggggtcaa caacgcaagc
      601 gagctcccct ttctttcttc cctactaaga actaacccaa acccatttag gatgactccg
      661 gtagccatct atcccgccct ttatcgagac aacaaagaca ccctccttga tgtccctttc
      721 gatctcagtc cccccggaat gaatatcccc ttttcagcaa acaagatgcg cgccattttt
      781 gtggcgacaa agatcctcta cccgcgtgag ttgcaagata agcctcgacc cgagaattct
      841 cagcccctct atcaagaggt ggttttcacc gatcggctaa cggcagacga aatcgcacgg
      901 gtgaaatacg aaaagtcgcc gatcgcaaag atgttcccga aggccccgat ccataccgac
      961 gtagaggtcc gcagtggcta ccgagtctgg gatgtggccc gggccgcatt ggcagatatg
     1021 tacctaacgg cgcccatgtg gctagaagac cccacgggcg gtcatcccaa agaacttttt
     1081 atcacagga
//
LOCUS       DATMKH010000553         1181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2163474, whole genome shotgun sequence.
ACCESSION   DATMKH010000553 DATMKH010000000
VERSION     DATMKH010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2163474"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..>1181)
                     /locus_tag="VNV63_06575"
     CDS             complement(125..>1181)
                     /locus_tag="VNV63_06575"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXC62322.1"
                     /translation="KLFKPFQQVDTSTTHRFGGTGLGLVISKRLAEFMGGTIWVESSP
                     DSGSSFFFSVILKASNAPIPAHQSPEPGLLGSHSVLIVDDNATNRRILEIQLKIWGMN
                     PSSVSGGGEGLKKMAEQTFDVVLIDFQMPGMDGVTLAREIRQRGPSPLILLSSSGEII
                     AGEDADLFHAQISKPIRHSTLFNALLNIIGTEVRQPLNIAEKKFDGTMATKHPLRILL
                     AEDNSVNQQVGLLMLSRLGYTADLATDGQRALNAAEKVGYDLILMDIQMPNMNGIDAA
                     RIIRERLGAKCPSIFALTAEALEGDKQRFLGLGFDGYMSKPLQIHTLQETLQMINPSV
                     IPMSYRLLVPSGLASRK"
BASE COUNT          260 a          320 c          319 g          282 t
ORIGIN      
        1 ccgcctatct gatgtaagta aggcgtcccg ccttaccttc ccgggttgaa accttgggct
       61 gagttctcca accgcttcgg ggcagttaaa agtcttgaga gaagcttttc tccgcgccgt
      121 gcgctcattt ccgtgatgcc aaaccagacg ggacaagcag gcgataactc atcgggatga
      181 cgctcgggtt gatcatctgc aacgtttcct gaagtgtatg aatttgcaga ggtttgctca
      241 tgtagccgtc aaagccgaga ccgaggaatc tttgtttgtc accttcaagc gcctctgcgg
      301 tcagcgcaaa aatagaagga cattttgcgc ccagacgctc ccggatgata cgagccgcat
      361 cgatcccgtt catattgggc atctgaatgt ccatcaggat caggtcgtac ccgaccttct
      421 ccgccgcatt cagagccctt tggccgtccg tcgccaagtc tgcggtatat cccagtcgtg
      481 aaagcatgag caaaccaacc tgctgattga cggagttatc ctccgccagc aaaatccgta
      541 gcggatgttt agtcgccatg gtgccgtcga actttttctc cgcgatattg agcggttgtc
      601 ttacttcggt accgatgatg ttcagcaacg cgttgaacag agtggagtgc ctgatcggtt
      661 tggagatttg ggcgtgaaag agatcggcgt cttctcctgc gataatctct ccggaggacg
      721 aaagcaggat gagcggcgat ggcccccgct ggcggatctc tcgcgccaga gtgacgccat
      781 ccattcccgg catttggaag tcgatcagaa caacgtcaaa ggtttgttcc gccattttct
      841 tcagaccttc gcctccgccg gaaacggagc tcggattcat tccccagatt ttcaattgaa
      901 tctccagaat gcgccgattg gtggcgttgt cgtccacgat gagaacactg tgggatccca
      961 ggagaccggg ctcgggagac tgatgagccg gaatcggcgc gttcgaagcc ttcaggatca
     1021 cggaaaaaaa gaacgatgat ccagagtcgg gactgctttc cacccagatc gttccgccca
     1081 taaattcggc caaccgttta ctgattacga ggccgagccc ggtccctcca aaccggtggg
     1141 tggtcgacgt atcgacttgc tgaaatggtt tgaaaagttt t
//
LOCUS       DATMKH010000554         3034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4430035, whole genome shotgun sequence.
ACCESSION   DATMKH010000554 DATMKH010000000
VERSION     DATMKH010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3034
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4430035"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="VNV63_06580"
     CDS             complement(<1..535)
                     /locus_tag="VNV63_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXC62323.1"
                     /translation="MTDFLVIGSGVAGLQAALEASRGGKVLMVTKGPVRESSSGYAQG
                     GVAVALRDEQDIESHYQDTLRSGNGLCRPKAVRVLVEEGARCIRQLIARGARFDKKGR
                     AFAWTREAAHSRDRILRARGDATGEEIVKTLVRQVKSRTQIGPLPHTFTLDLVMIEGV
                     CRGAVVLDQRKQTVSLIW"
     gene            complement(544..1065)
                     /locus_tag="VNV63_06585"
     CDS             complement(544..1065)
                     /locus_tag="VNV63_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62324.1"
                     /translation="MQTSVKPVCYLVFLLLPIFLALVSCSKEAVKPSEDDLKIRTVLS
                     FVDRLKQSYEAKDQAGLLALISPDSTMLSSLPPILARDFQSFDRIRLTPMVDRIEIDK
                     GKVTVVLNWDGQWQNGTTQLIYKEKGTTILKLKESPAIRLMELSGDPLFGAGTRSSQT
                     SPASRNSNSRPNP"
     gene            1199..1288
                     /locus_tag="VNV63_06590"
     CDS             1199..1288
                     /locus_tag="VNV63_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003792170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AURKAIP1/COX24 domain-containing protein"
                     /protein_id="HXC62325.1"
                     /translation="MSRVIKKRRKKMRKHKYRKLRKKMRHQRK"
     gene            1332..1553
                     /locus_tag="VNV63_06595"
     CDS             1332..1553
                     /locus_tag="VNV63_06595"
                     /inference="COORDINATES: protein motif:HMM:NF044585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62326.1"
                     /translation="MSTSRKVRVKVRTVNATYEGDLLIPPMRNRVSDLLNEEIRVFIN
                     LTDVAIDGGPRNVAFVSLNKNLMESIIEE"
     gene            1557..1832
                     /locus_tag="VNV63_06600"
     CDS             1557..1832
                     /locus_tag="VNV63_06600"
                     /inference="COORDINATES: protein motif:HMM:NF013851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HXC62327.1"
                     /translation="MISNDMLAGYIKKTIPDAMVSVIDRTGTMDHFIIRVVSETFKDK
                     SLLDRHRLVYQSLNEPMSDGRIHALEIQAQTHEETSGTAAQGLRIQS"
     gene            1861..2184
                     /locus_tag="VNV63_06605"
     CDS             1861..2184
                     /locus_tag="VNV63_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005009277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HXC62328.1"
                     /translation="MSNPIEEEVQKEIKGNKILIYGKGTKSAPRCGFTMETIQFFDRY
                     GYPYEVIDVLENPAKREHLSKMTNWPTLPKVFINGTFYGDTDILEPMAKKGELEPLFK
                     EAFKK"
     gene            complement(2215..2907)
                     /locus_tag="VNV63_06610"
     CDS             complement(2215..2907)
                     /locus_tag="VNV63_06610"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HXC62329.1"
                     /translation="MDEQLKLAEMQTGPGFLINLLHFIVLSFLDLLFRVLNRTKVIGQ
                     ENIPKRGERGVMILSNHISALDPLLIGITAMPRFSPIWWRAAAKEELFTTPFSRFVLQ
                     IIGAFSVKRGRHDAGSMDRMAQVLSTSVLVVFPEGTWSTTGQLLPGRLGVGNIIYNAR
                     PAKILPVAVKGTDDILPRDHALPRIGRRARIIYGPPMDMNRFYLQPDTRETAREIVQA
                     VMAEIGRLYSQI"
     gene            complement(2892..>3034)
                     /locus_tag="VNV63_06615"
     CDS             complement(2892..>3034)
                     /locus_tag="VNV63_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HXC62330.1"
                     /translation="GLVYHLPEEITLIASYHPSQQNTLTGRLTRPMFHAVFRQARRWM
                     NN"
BASE COUNT          772 a          755 c          837 g          670 t
ORIGIN      
        1 cccagattag cgagacggtc tgtttccgct gatccagtac aactgctccg cggcagacac
       61 cctcaatcat aacaagatcc agtgtgaatg tatgaggcag cgggccgatc tgagttctag
      121 actttacctg ccgaacgagt gtttttacga tttcttcgcc tgtggcatcc cctcgagcgc
      181 gtaagatccg atcccggctg tgagcggctt cccgggtcca ggcaaatgct cgaccctttt
      241 tatcgaaccg ggcgccccgg gcgatgagct gacggataca tcttgcccct tcttcgacaa
      301 ggaccctgac cgctttcggg cggcaaagac cattcccgga gcggagcgtg tcctgatagt
      361 gactctcgat gtcctgctca tcccgtaggg ccacggccac accaccctga gcgtaaccgg
      421 aactgctctc tctcacaggt cccttggtga ccatgagaac cttgccgccc cggctggcct
      481 ccagtgcggc ctgtagaccg gcgacaccgc ttccgatgac caaaaaatcc gtcacagatt
      541 gtttcatggg tttggacggc tgtttgaatt gcgggatgcc ggagaggtct gagagctacg
      601 agtcccggct ccgaagaggg gatctcctga aagctccatc aatcggatcg caggagattc
      661 tttaagcttg aggattgtgg tccccttttc tttgtagatc aattgtgtgg tcccgttctg
      721 ccattggccg tcccagttta gtaccacggt tactttgccc ttgtctatct cgatccgatc
      781 caccataggc gttagtctga tacgatcgaa cgattgaaaa tcacgggcaa gaatcggtgg
      841 cagcgatgag agcatcgtag agtcgggaga gatcaaggcc aatagcccgg cttgatcttt
      901 tgcttcataa gattgcttca aacgatctac aaaggagaga acagtccgga ttttaagatc
      961 gtcctcagaa ggcttgaccg cttctttgga gcagctcacc agcgcgagaa agatgggcaa
     1021 tagaaggaac acaagataac aaacaggctt gacggatgtt tgcatgagac gattatacgg
     1081 gcaactattc gaaaaggtca agcaccggaa aattccctgt tgcctttcaa ggggaatgtg
     1141 atacaataac aagctgatta gtaaggtcca cagtgaaatt tttgggacgg agggcagaat
     1201 gtcgcgtgtg atcaagaaac gaagaaagaa gatgcgaaag cacaagtacc gtaagctccg
     1261 caagaagatg aggcatcaac gtaagtaggg agggcgttag tcacgcttaa gtgatggagg
     1321 caaagaagcg aatgagcaca tcgagaaagg tgcgggtgaa ggtacggacc gtcaatgcca
     1381 cctatgaagg cgatctgctg atacctccaa tgcggaatcg ggtctcggat cttctcaatg
     1441 aagagatacg ggttttcatc aatctcacgg acgtcgcaat tgatggggga cctagaaatg
     1501 ttgcgtttgt gtcgttgaat aaaaacttga tggagtccat cattgaggag tgataaatga
     1561 tcagcaatga tatgctggct ggctacatta agaaaacgat tcctgacgcg atggtctctg
     1621 tcatagaccg gaccgggacg atggatcatt tcatcatccg ggtggtctcg gagaccttta
     1681 aggataagtc attactggat cggcatcgcc tggtctacca atcgttaaac gaaccgatga
     1741 gcgatggtcg gatccatgct ctcgagattc aggctcagac gcatgaagaa acatccggga
     1801 cggctgccca gggattaagg atccagagct agatccaagt aagaacgcaa ggaggaaaac
     1861 atgtcaaatc caatcgaaga agaggtacaa aaagagatca aagggaataa gattttgatt
     1921 tacggcaagg gaacgaaaag cgctccgaga tgcggcttca caatggagac gatacagttt
     1981 tttgaccgat acgggtatcc ctacgaggtt attgacgttc tggaaaaccc ggctaaacgg
     2041 gaacacctct ccaagatgac caactggccg acccttccca aggtcttcat caacggtacg
     2101 ttttacggcg ataccgatat cctggagccg atggccaaaa agggcgagct cgagccgctg
     2161 tttaaagaag ccttcaagaa ataaactctt atcctgccgc tttgcattca gaacttagat
     2221 ctgcgagtag agccttccga tctcggccat cacggcctgt acgatctctc gtgcggtctc
     2281 tctcgtgtcc ggctgaagat aaaaacggtt catgtccata ggtgggccgt agataattcg
     2341 tgcgcgccgg ccgatgcggg gcagcgcatg atcgcgcggt aagatgtcgt ccgttccttt
     2401 gaccgcaacc ggcaggatct tggccggccg ggcgttgtag attatattcc cgactccgag
     2461 tctgccgggg aggagctggc cggttgtaga ccaggttccc tccggaaaca ccaccaggac
     2521 actggtcgaa agaacctgcg ccatccgatc catagatccg gcgtcatgcc gaccccgttt
     2581 aaccgagaag gccccgatga tctggagcac aaatcgcgaa aaaggggttg tgaaaagctc
     2641 ctcctttgcg gctgcccgcc accaaatcgg tgaaaaacgc ggcatcgccg tgatcccaat
     2701 gaggagtggg tccagggccg agatatggtt tgacaggatc atgacgcctc gctcgccgcg
     2761 tttggggatg ttctcctgcc cgattacttt agtacgattc aagaccctaa ataaaagatc
     2821 aagaaagctg agcacgatga aatgaaggag gttgatcaga aacccggggc cggtttgcat
     2881 ctcggccagc ttcaattgtt catccaccgg cgggcttgac ggaagacagc atgaaacatc
     2941 ggccgtgtca ggcgccccgt tagggtgttc tgctggctcg gatgatagga tgcgatgagc
     3001 gtgatttcct ctgggagatg ataaaccagg ccgt
//
LOCUS       DATMKH010000555         1140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4098286, whole genome shotgun sequence.
ACCESSION   DATMKH010000555 DATMKH010000000
VERSION     DATMKH010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4098286"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..>1140
                     /locus_tag="VNV63_06620"
     CDS             121..>1140
                     /locus_tag="VNV63_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62331.1"
                     /translation="MVPLLQKYRVPYVDGREITLEHKGAFPVRAYPKTGTHWTRAVAF
                     FTTAALLKTIERESGRKMPQLSEAVESIDHRPDSRPIPANPEQRRTGKIPIESDSVDD
                     DLFKLLNLIQKPNQRYLHASFQIPEGWQKKSGVLTIVGGSYMSAILDSLDSAKVFERM
                     NHYSYFRDSRHRFPDNVLDPVDENAIPWEEDFWNTTAVVLEANEEAMARRHLPAFLMA
                     ALAALQQHGPQQPAADDPPRPLSWGFGAGENGSALAKKGFNAPEHQLTWISRHDAEID
                     LPSPAKNTELQLIVEAMPFLGDDAAERIVKVEANGIPIGALDLVDPSVQFYSLTIKAI
                     ANTAPS"
BASE COUNT          289 a          295 c          296 g          260 t
ORIGIN      
        1 ggatcgtgct ttgtttttgt tattacgccc agtaaagcga ctatttgtcc ggaggacatt
       61 cctgatcgat accttccaaa gtctgaaggc agagagcgaa gattaaccaa ttacgaagtc
      121 ttggttcctc tattacaaaa gtaccgcgtt ccatacgtgg atgggcggga gatcacgttg
      181 gaacacaaag gcgcttttcc agtgcgagcg taccctaaga ccggcactca ttggacacga
      241 gcagtagcct ttttcaccac ggctgctctt ctgaaaacca ttgagcggga atcaggacga
      301 aagatgccgc aattgtcgga ggcggtcgaa agcatcgatc accgtccgga ttcacgccca
      361 attcccgcca atccggagca gcgaagaacc ggaaagattc cgatcgaatc ggattctgtc
      421 gacgacgacc tgtttaagtt actcaatctc atccagaagc ccaaccagcg ctatcttcat
      481 gctagctttc agattccgga gggttggcaa aaaaagagcg gcgtcctgac cattgtcgga
      541 ggcagctata tgtcagcgat tctggattct cttgatagcg ctaaggtgtt cgaacgtatg
      601 aaccattact cttatttcag agattccaga caccgatttc ccgataatgt cctcgaccct
      661 gtcgatgaaa atgccattcc gtgggaagaa gacttttgga acaccacggc agtcgttctc
      721 gaggccaacg aagaagcaat ggcaagacgg catcttcccg cgttcctaat ggccgcattg
      781 gcggcgcttc agcagcatgg accacaacaa ccggcagcgg atgatcctcc gcgaccgctt
      841 tcctggggat ttggtgcggg cgaaaacggg agcgccctgg caaagaaagg gttcaatgct
      901 ccagaacatc agctgacctg gatttcgagg cacgatgctg agatcgattt accgtcgccg
      961 gcgaaaaata cggagctcca attgattgtg gaagcgatgc ccttcctggg agacgatgcc
     1021 gccgagagga tcgtgaaggt agaggccaat gggattccta ttggggcgct agacctggta
     1081 gatccttcgg tccaatttta ttcactcacc atcaaagcta ttgcaaatac ggctccctct
//
LOCUS       DATMKH010000556         1898 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2828833, whole genome shotgun sequence.
ACCESSION   DATMKH010000556 DATMKH010000000
VERSION     DATMKH010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2828833"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="VNV63_06625"
     CDS             <1..673
                     /locus_tag="VNV63_06625"
                     /inference="COORDINATES: protein motif:HMM:NF022932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TrmB family transcriptional regulator
                     sugar-binding domain-containing protein"
                     /protein_id="HXC62332.1"
                     /translation="SKTKHYVPIQPDEFLSRYKSTVGTLVDSLKEEFSSLNSCQDVSH
                     IWNISDYDHLIDKAHRMIDSACSVLLLSLWKEELDSLETALRRAEGRGVQIALVHFGL
                     PKLEIGQVYYHPIEDTLYAEKGGRGFAVIADSQEVLIGTVSYEGKTEGAWSRNKGFVM
                     IAEDYVKHDVYITKIIKRLDKELIDCFGERYAKLRDVLRDEEVDRGNASKIQEKGVLT
                     SHEIR"
     gene            660..>1898
                     /locus_tag="VNV63_06630"
     CDS             660..>1898
                     /locus_tag="VNV63_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit alpha"
                     /protein_id="HXC62333.1"
                     /translation="MKSAKRSGLPPKQGLYDPRYEKEACGIGFVANVKGNKSHEIVRK
                     GLEVLENLAHRGAVGCDPCTGDGAGILIQIPHEFLKRACGEIGIRLPGAGEYGVGMGF
                     LPKDPSQRHLCETLISRIIQEEGQRLLGWRDVPAKDEHLGELARSSQPHIRQFFIARD
                     ILDEAQFERKLYVIRKRIENAVLNSAIEDRASFYINSLSSSTIVYKGLLLPRQIPLFY
                     PDLADPTVMSALAMVHSRFSTNTFPTWARAHPYRYACHNGEINTLKGNINWMQARQGR
                     LASELFGDDIQKLYPIIYEDQSDSACFDNALEFLVMGGRSLPHAVMMLIPEAWAGNPD
                     MDLDRRGFYEYHAAMMEPWDGPAAMLFTDGKLIGATLDRNGLRPCRYLVTHDDFAVVA
                     SEAGVLSFKPEEVLTKGRLKP"
BASE COUNT          444 a          521 c          518 g          415 t
ORIGIN      
        1 cagcaagaca aaacattacg ttccgatcca accggacgaa ttcttaagcc gatacaaaag
       61 cacagtcgga acgcttgtag actctctaaa agaagagttc agcagcctca acagctgtca
      121 ggatgtaagc cacatctgga atatctccga ttatgaccat cttatagaca aggcacatcg
      181 gatgattgac agcgcctgtt ccgtgcttct cctatcgctc tggaaggaag agttggactc
      241 gcttgaaacc gctctccgta gagccgaagg ccgtggagtc cagatcgccc tggttcattt
      301 cgggcttcca aaactggaga taggtcaggt atactatcat ccaatagagg acacgttgta
      361 cgctgagaaa ggcggccgcg ggtttgccgt gatcgctgat tctcaagagg tcttgatcgg
      421 aaccgtcagt tatgaaggca agaccgaagg cgcctggagt cgtaataaag ggttcgtcat
      481 gattgccgag gattatgtaa aacatgacgt ctatatcacc aagatcatta agcgcctcga
      541 taaagaactg attgactgct ttggcgaaag atatgcgaag cttcgtgatg ttctccgcga
      601 tgaggaagta gatcgtggaa atgcttctaa gattcaggag aaaggagtct taacctctca
      661 tgaaatccgc taagcgatcc ggcctgccgc ctaaacaggg tctctacgat ccccggtacg
      721 agaaagaagc ctgcggaatc gggtttgtgg ccaatgttaa aggcaacaaa tcccacgaga
      781 tcgtccggaa gggacttgag gttttggaaa accttgcgca tcgtggcgcg gtaggctgtg
      841 atccctgcac aggagacggc gcgggcatcc tgattcagat tccccacgag tttctaaaaa
      901 gggcctgtgg cgagatcggc atacggcttc ccggcgcagg cgagtacggc gtggggatgg
      961 gcttccttcc caaggatcct tctcagcgtc atttgtgcga gacgctgatt tctcggatca
     1021 tccaagaaga gggccagcgt ctgcttggat ggcgggatgt cccggcaaag gatgagcacc
     1081 tgggtgaact tgcacggtcc agccagcccc atatccgcca gttcttcatc gcgcgtgaca
     1141 tcttagacga ggcacagttt gaacgaaaac tctacgtgat tcgaaagcgc atcgagaacg
     1201 cggtcttgaa ctccgcaatc gaggatcggg cctctttcta cattaacagc ctgtcttcct
     1261 ccacgattgt gtacaagggg cttctgctcc cccgtcagat accgctcttc tatccggacc
     1321 tggccgatcc gaccgtgatg agcgcgctgg ccatggtcca ttcccgtttc tcgaccaaca
     1381 catttccgac atgggcccgt gcacatcctt accgctacgc ctgccacaac ggtgagatca
     1441 acacgctcaa agggaacatc aactggatgc aggcgcgtca gggccggctc gcgtccgaac
     1501 tgtttggcga tgacatacag aagctttatc cgatcatcta tgaagaccag agcgactcgg
     1561 cctgttttga caacgccctg gagttcttag tgatgggcgg ccgctctctt cctcacgccg
     1621 tgatgatgct catcccggag gcctgggccg gaaatcctga catggacctg gatcggcgcg
     1681 gcttctacga atatcacgcc gcgatgatgg aaccctggga cggccccgcc gccatgctgt
     1741 tcacggacgg caagctgatc ggtgcgacgc tggaccgtaa cggcctgcgc ccctgccgct
     1801 atcttgtaac acatgacgac ttcgcggtgg tcgcctccga ggccggcgtg ctttctttca
     1861 agcctgaaga agttctgacc aagggccgcc tcaaaccg
//
LOCUS       DATMKH010000557         1586 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_904392,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000557 DATMKH010000000
VERSION     DATMKH010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_904392"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VNV63_06635"
     CDS             <1..232
                     /locus_tag="VNV63_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62334.1"
                     /translation="DDFWGGKDDQLQAKRARGTRLLGFGAFLQELQNSCCLLAGGLSL
                     VFSPTLKSLQRNSDGLGYFGRLLEDNIPVVPK"
     gene            complement(409..1176)
                     /locus_tag="VNV63_06640"
     CDS             complement(409..1176)
                     /locus_tag="VNV63_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62335.1"
                     /translation="MAQINPLPTSLSPSVPDRPGEVAELKGIIFQSDAVNILGAWIIR
                     PVETLDLQLAGLGRSPGTPPLAMHAGLHVVLRDRREFVVEQLFGTPREDFVDGLNWTP
                     LETFRARDHKGWDVTVQATAFRGVDENVEQEAIAFLNHIHGRPFFGEDCTTLIERAFG
                     KRRLFADSPTASSIGFGMRVGDPALPLLKPDVRLAPDAERLLRADMLRGLHDPTEDWD
                     APNGHLWIRRGLGWLLFFAVVVGLGFFTKKRTRRAVK"
     gene            complement(1304..>1586)
                     /locus_tag="VNV63_06645"
     CDS             complement(1304..>1586)
                     /locus_tag="VNV63_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62336.1"
                     /translation="GSVASVRFQSISQQDHNFLDEILQNQITGDQKQGQGRIVGSGDV
                     LVLKGELVVNSPGGKRSELIAHKEAVRRAQLVNHNEMLRRVHQGRSKQQ"
BASE COUNT          341 a          449 c          405 g          391 t
ORIGIN      
        1 ggacgatttt tggggaggca aagatgacca actacaagcc aaacgcgcga ggggaacgcg
       61 cctactcggt tttggcgctt tccttcaaga actccaaaat tcctgctgct tgctcgctgg
      121 tgggctctcc cttgtcttca gtccaacctt gaagagtctc cagcgaaact ccgatggcct
      181 gggctacttt ggacgcctgt tggaggataa tatcccggta gttcccaagt gaagtgagtt
      241 gttctttggt cgcttgcatg acctaacaat accagaaatc ggggtaatta gcctcacgca
      301 gaactgagca acctgatttt ctatcctcca atgcggccct tctggatcga gtccctccaa
      361 cctggcgaac ggctgatcca atcgaatccc actccgatgg ataccgcttc atttgaccgc
      421 gcgtctggtt cgcttcttcg taaagaagcc aagcccgacc acgacggcaa agaatagcag
      481 ccatcccaga ccgcgccgga tccagaggtg accgtttgga gcatcccaat cttctgtcgg
      541 gtcatgcagt ccgcggagca tatcggctct caggagacgt tcggcatccg gagcgagtct
      601 tacgtccggc ttgagcaatg gtaaggcggg gtcgcctacg cgcatcccaa agcctataga
      661 ggaagccgtc gggctgtcgg caaacagtcg ccgctttcca aaagcccgtt cgatcagcgt
      721 cgtgcaatcc tcgccgaaaa aagggcggcc atggatatga ttcagaaatg cgatcgcctc
      781 ctgttcgaca ttctcatcga ccccgcgaaa tgccgtggcc tgcacagtca cgtcccagcc
      841 tttatgatcg cgggctctga acgtttcgag cggcgtccag tttaatccat cgacgaagtc
      901 ttcgcgcgga gtaccaaaca attgttcgac tacgaattcc cttctatccc ggaggacgac
      961 gtgaagtccg gcatgcatgg ccagtggcgg agtgcccggg gaacgaccta atcctgcaag
     1021 ttgcagatcg agagtttcca ccggacgaat gatccatgcg cccagtatgt tcacggcatc
     1081 actttggaaa atgattccct ttaattcggc aacttcgcct ggccggtcgg gtacagaggg
     1141 agacaacgat gttggcaaag gattgatctg cgccacggcc gaatctttaa ccgggtttgc
     1201 tccagatgca aagtgtgccg tgccctgtac tcaagggttc caccctttcg acccggttcc
     1261 gggttctcag ttttcttcct tgagtggtct tttcatccta tcatcactgt tgttttgatc
     1321 gaccttgatg aacgcgtctg agcatttcat tatggttgac cagttgagcg cgcctgaccg
     1381 cctctttgtg tgcgattagt tcggagcgct tacctcctgg actattgacg actaactccc
     1441 ccttcaggac caacacatcc ccagatccaa cgatccttcc ttggccttgt ttttgatcac
     1501 ccgtgatttg gttctgcaga atttcatcta gaaaattgtg atcttgttgg ctgatgcttt
     1561 gaaaccggac ggaggctaca gatccg
//
LOCUS       DATMKH010000558         1213 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4431029, whole genome shotgun sequence.
ACCESSION   DATMKH010000558 DATMKH010000000
VERSION     DATMKH010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4431029"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="VNV63_06650"
     CDS             <1..402
                     /locus_tag="VNV63_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HXC62337.1"
                     /translation="VGFQIGGQATDLVLLVMNERGASSILSSKVKLGADASAAAGPKG
                     RDASADTDAYLRAEILSYSRTRGLFAGISLEGSTLRPDDDASADVYGHKITAREIVLG
                     NKTSIPASGRHLVRVLQKNAPHNQSQKASNQ"
     gene            678..>1213
                     /locus_tag="VNV63_06655"
     CDS             678..>1213
                     /locus_tag="VNV63_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62338.1"
                     /translation="MLSRVSITLILILFSTSLISAQDLSKYRNFSFGMSVASVSKQAD
                     QQIAKAEVIHQRPALIEESTWYPPQPFGSSRPAEPVEKVLFSFYNGELYRMLVTYDNS
                     AIKGLTDEDMIRVISAKYGASTRPVANVNFPMNPSYRATEKVVARWEDSQFSFNLFRS
                     STDTFALVMFDKRMDAQAG"
BASE COUNT          289 a          318 c          323 g          283 t
ORIGIN      
        1 gtcgggtttc agatcggcgg acaagcgacg gatctggtcc tcctggtcat gaatgagaga
       61 ggagcatctt ccattctcag cagcaaggtg aaacttggcg ccgatgcctc cgcggcagcg
      121 ggtccaaaag gacgcgacgc gtcagcagac accgatgctt acttgagggc ggaaatcctg
      181 agctactccc gcacacgtgg tctttttgcg ggaatttctc tcgagggttc cacgcttcgc
      241 ccggacgatg atgccagcgc ggatgtctac ggccacaaga tcacagcgag agaaattgta
      301 ctgggaaaca aaaccagcat tcccgcctca ggtcgccatc tcgttcgcgt tttgcagaag
      361 aacgcaccgc acaatcaatc acaaaaggcc tcgaaccagt agttgcagtc gtcacggggt
      421 tttttcttgt gctggagggt ggagcaggac ctcatgttca tttcgacgag gcttgctcca
      481 ccggcagccc gaagaagttg acaatatttg gccctcgcgt tgccacgaag agttttcaca
      541 tgaatgaatc ccgctagcgg cgtgcagttc accaggtaat gacctagctc ttgcacagcg
      601 caattagttc tgggcggaac aaagcccctg atctagcctg gtgaagtatg agtcacaaag
      661 gagataccaa agtgaagatg ttaagccgag tatcgatcac gttaatcctg attttatttt
      721 ctacctcttt gatttccgca caggatctgt ccaagtaccg gaatttctca ttcgggatga
      781 gtgtggccag tgtctcaaag caggcggacc agcagatcgc gaaggcggaa gtcattcatc
      841 agaggccagc tttgattgaa gagtcgacgt ggtatccgcc acaacccttt ggatcttcac
      901 ggccagcgga acccgtcgag aaggttctct tctctttcta caacggggaa ctctaccgga
      961 tgttggtgac ttacgacaat tccgccatta aaggtctgac cgatgaagac atgatacgcg
     1021 ttatttccgc gaagtacggg gcctctacga ggcctgtcgc gaatgtcaat ttccctatga
     1081 acccatccta tcgggcgacc gaaaaagtgg tcgcacgctg ggaagactct caattttcat
     1141 tcaacctgtt tcggtcctcg acggatactt tcgcgctagt catgtttgat aaacggatgg
     1201 atgcccaggc cgg
//
LOCUS       DATMKH010000559         1359 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4724934, whole genome shotgun sequence.
ACCESSION   DATMKH010000559 DATMKH010000000
VERSION     DATMKH010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4724934"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..828
                     /locus_tag="VNV63_06660"
     CDS             133..828
                     /locus_tag="VNV63_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007432056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXC62339.1"
                     /translation="MNKHIAVVTGANRGIGFEIVRQLAKKGWIVVLTARDEAKGRAAC
                     EQLKKQGLDVEFRVLDVANPESIHRLKNDLEKTFGRLDVLVNNAGIFIDRTKNSLNVD
                     MKTVRETMETNFFGPLQLSQTLLSLLLKSDDARIINISSGLGQLSDGGGGYPSYSISK
                     AALNMMTVQMAEDLKGRVHVNSVCPGWVRTEMGGPGATRSVEEGTDTAVWLATEPNVP
                     NGKFIRDRKEIAW"
     gene            848..1246
                     /locus_tag="VNV63_06665"
     CDS             848..1246
                     /locus_tag="VNV63_06665"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXC62340.1"
                     /translation="MRTFGYFTTIAVIAVLFVMLSIQGLQAQADSKKIFPALDVPVSS
                     GAQTQNSNGIKLYNEGKWTEAAQAFREAVRAADESAEAHYNLALALDKLQQHKEATQE
                     FGHAIKLAPDNPAITESEILKAHLAMKEKN"
BASE COUNT          366 a          333 c          365 g          295 t
ORIGIN      
        1 gtaaactact taaataaggt tttagatata cccaataatc gtcggaaagc ctacctactt
       61 tattccttcc tttaactaag ctataatccc ttgtatctac gggaaacata gaatctataa
      121 aagaggattt aaatgaacaa acacatcgcg gtggtgacgg gggccaaccg gggaatcggg
      181 tttgagatcg tccggcagct ggctaagaaa ggctggattg tggtactgac cgcgcgggac
      241 gaggcaaaag gccgtgcggc ctgcgaacag ctcaaaaagc aaggtctgga cgtcgagttc
      301 cgtgtactgg atgtggcgaa tcctgagagc atacatcggc tcaagaatga tctggaaaag
      361 acattcgggc ggctggatgt tctggtgaac aatgccggca tcttcatcga cagaactaaa
      421 aattcactga atgtcgatat gaaaacggtg agagagacga tggagacaaa cttcttcgga
      481 cccctccaac tttctcagac gctgctctct ctgctcctga agagcgacga tgcgcggatc
      541 atcaacatct ccagcggcct gggacagttg agcgacggcg ggggcggcta cccttcctac
      601 agcatttcaa aggcggcgct caatatgatg acggtccaga tggccgaaga tcttaaaggc
      661 cgggtccatg tgaattcggt ctgccccggc tgggtccgga ccgagatggg cggtccgggg
      721 gcaacccgaa gcgttgaaga ggggaccgac accgccgtct ggctcgcgac cgagccgaat
      781 gttcccaacg gaaaattcat acgtgaccgg aaagagattg cgtggtgaaa aagaatggag
      841 gtttcagatg cgtacattcg gatactttac gacaatagcg gttattgcgg tattattcgt
      901 gatgctttct atacaaggac ttcaggctca ggccgattca aagaaaatct tcccggcttt
      961 ggatgtcccg gtgagctcgg gagctcagac gcaaaattcc aacggcatca aactgtacaa
     1021 tgaagggaaa tggacggagg cagcgcaagc cttccgagaa gcggtccggg ccgccgacga
     1081 atccgctgag gctcattaca accttgccct tgcgttagat aaactgcaac agcacaaaga
     1141 ggccacgcag gagttcggcc acgcgatcaa gctggcgccc gataatccgg ccattacgga
     1201 atccgagata cttaaagccc atcttgcgat gaaggaaaaa aactaaaaaa ccctggcggc
     1261 gatcggttct cgggtgttag gagcaccaat cttttccgta tagcactttt cacacattgc
     1321 acctactcta tttcctttta agatcttttc acatgcgtt
//
LOCUS       DATMKH010000560         3645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1855512, whole genome shotgun sequence.
ACCESSION   DATMKH010000560 DATMKH010000000
VERSION     DATMKH010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3645
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1855512"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..1130)
                     /gene="prfA"
                     /locus_tag="VNV63_06670"
     CDS             complement(33..1130)
                     /gene="prfA"
                     /locus_tag="VNV63_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018212048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HXC62341.1"
                     /translation="MVLVKEQLLTQKLEAIVQRYEQVGQLLSDRTVVANFQEVQRLGK
                     ERAELMPMVKAYQEHRQLLKAMAEAQVILETSKNEPGLGELAERELRELKNQQSVLEE
                     RIKGLLVPRDLRDEKNTLLEIRAGTGGGEAALFAAELFRMYTKYAERRGWRVELVSSS
                     PTGIGGFKEVIAIIEGKGTYSRFKYESGVHRVQRVPATEAGGRIHTSTVTVAVLPEAE
                     EVDIQIDLKDLRIDTFCSSGPGGQGVNTTHSAVRILHQPSGLIVSCQDERSQIKNRAK
                     GMRILRSRLLEMEQQKQNDEIAKARRSQVGTGDRSEKIRTYNFSQNRITDHRAGLSLH
                     KLEAVLEGELDELIEALTAWDHAERLQKSLS"
     gene            complement(1161..1364)
                     /gene="rpmE"
                     /locus_tag="VNV63_06675"
     CDS             complement(1161..1364)
                     /gene="rpmE"
                     /locus_tag="VNV63_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005352672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HXC62342.1"
                     /translation="MKPGIHPEYKPATIRCACGNAIKTRSTRGDMKVEICSACHPFFT
                     GTQKIVDTEGRVERFKKKYIKKK"
     gene            complement(1506..2753)
                     /gene="rho"
                     /locus_tag="VNV63_06680"
     CDS             complement(1506..2753)
                     /gene="rho"
                     /locus_tag="VNV63_06680"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HXC62343.1"
                     /translation="MNLAELKEKSIAELNEVAKELKIEGASNLRKQELIFAILQAQTE
                     KNGVIYGEGVLETLPDGFGFLRAPDYNYLPGPDDIYISPSQIRRFNLRTGDIVSGQIR
                     PPKESERYFALLKVEKINLEDPEIARDKILFDNLTPLYPMERIRLEYATDDYNTRVME
                     LVTPIGKGQRGLIVAAPRTGKTMLLQALAKAVLKNHPEIVLIVLLIDERPEEVTDWQR
                     QVKAEVVSSTFDEPPQRHAQVAEMVLEKAKRLVEHKKDVVILLDSVTRLARAYNTITP
                     PSGKVLSGGLDSNALQRPKRFFGAARNIEDGGSLTIMATALVDTGSRMDDVIFEEFKG
                     TGNMEIHLDRRLADKRVFPAFDISLSGTRKEELLTEKDELNKMWILRKVLSPLSPVES
                     MEFLLDKIKGTKSNKEFLESMNK"
     gene            complement(3226..>3645)
                     /locus_tag="VNV63_06685"
     CDS             complement(3226..>3645)
                     /locus_tag="VNV63_06685"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HXC62344.1"
                     /translation="WLGKRLPTEAEWEKASRGTDGREYPWGNDFDGKKANTGDAGLGD
                     FAPVGSFETGKSPYGVYDMSGNVWEWTTDWYQPYPGNTYHSDAFGQKFKVLRGGSWGG
                     PGHYALSIFYRTSYRLYAPPEEAYPDAGFRCVKSVSE"
BASE COUNT          816 a         1007 c          843 g          979 t
ORIGIN      
        1 aagacagcct tgaaagccat ctttacctta actcaggata gagacttttg aagacgctcc
       61 gcatggtccc aagcggtcag ggcctcgatg agttcatcca actcgccttc caacacggcc
      121 tccagtttgt gaaggcttaa acccgcacgg tgatcggtta tccgattctg tgaaaaatta
      181 taggtccgta tcttctcgct tcggtctcct gtccccacct gggatcgccg tgccttggcg
      241 atctcgtcat tctgtttctg ttgctccatc tccaagagtc gagaacgaag gatccgcatg
      301 cctttggccc tgtttttaat ctgagaccgt tcatcctggc agcttacgat aagcccgctt
      361 ggctggtgta ggatgcgaac cgccgaatgg gtggtattga ctccctgacc acccggcccg
      421 gaggaacaga aggtatcaat ccgaagatcc tttagatcaa tctggatgtc gacctcttct
      481 gcctcgggca gaaccgccac cgtgacggtc gaggtgtgga tccggccgcc ggcctcggtt
      541 gcggggaccc gctgtacccg atggaccccg ctttcatact tgaagcggct gtaggtccct
      601 ttcccctcaa tgatcgcgat cacttctttg aagccgccga tccccgttgg actcgagctg
      661 acgagctcaa cgcgccaccc acggcgctcg gcatattttg tgtacatccg aaagagctca
      721 gccgcaaaca atgccgcttc tcctccgcca gtgccggcac ggatctccaa gagagtgttc
      781 ttctcgtctc tcaagtcccg cggcacgagc aaacctttga tgcgttcctc taaaaccgac
      841 tgttgattct taagctcccg gagttcccgt tccgccaact ctcccaagcc cggctcgttc
      901 tttgaagtct caaggatcac ttgagcctcg gccatcgcct tgagcaactg acgatgctct
      961 tgataggcct taaccatcgg cattagctca gcgcgttctt tgcccagacg ttggacctcc
     1021 tggaaatttg cgacgaccgt acggtccgac agaagttgcc cgacctgctc atatctctga
     1081 acgatcgcct ctaatttttg ggtgaggagc tgttctttga ctaaaaccat ttttaacccc
     1141 tggtcgagga ttcgtcttcg ttacttcttc ttgatatact tcttcttgaa tcgctcaacc
     1201 cggccttcgg tatccacaat tttctgcgtt ccggtaaaga acggatgaca ggccgagcag
     1261 atctctacct tcatatcacc ccgagtggaa cgggtcttga tcgcattacc gcatgcacaa
     1321 cgaatcgtgg ccggtttgta ttccggatga attcctggtt tcatcgttcg tcactcctta
     1381 tctacatgat ttaatagtaa tcatggggag gtaaaataaa aataccatcc cctgacttag
     1441 caaagaccca tactatatca tatttatgaa aattctggaa gggaagtagg acagggcagg
     1501 cccgcctatt tgttcatgga ctctaaaaat tccttgttgg atttggttcc tttgatcttg
     1561 tccaaaagga actccatgct ttctaccggg ctgagcgggc tcagtacctt ccgcaagatc
     1621 cacatcttat tgagttcatc cttttcggtc aggagctcct ccttccgcgt tccagagaga
     1681 ctgatatcaa aggctgggaa gacccgttta tcggcaaggc gtcggtccaa atggatctcc
     1741 atgttaccgg tacctttgaa ttcttcaaag atgacatcat ccatccggct tccggtatca
     1801 accaaggccg tggccatgat ggtaaggctt ccaccatctt cgatattgcg ggcggcaccg
     1861 aagaatcgct tgggtcgctg tagtgcattg gaatccaatc cgcctgagag caccttgccg
     1921 cttggtgggg taatcgtatt atacgcccgg gccaaacggg tgacgctatc caacaagatt
     1981 acgacatctt ttttgtgttc caccaaacgc tttgcttttt ctaacaccat ctcggcgacc
     2041 tgggcatgcc gttgcggcgg ctcatcaaag gtggagctca ctacctccgc cttgacctgt
     2101 ctctgccagt cggtgacctc ttccggccgc tcatctatta aaagcacgat caggacgatc
     2161 tccggatgat tcttaaggac agccttcgcg agcgcctgaa gcagcatggt cttcccggtt
     2221 cgaggggccg ctacgatgag accgcgttgc ccttttccga tcggggtgac caactccata
     2281 acccgcgtgt tgtagtcatc cgtcgcatac tccaaacgga tacgctccat tggatagagc
     2341 ggggtcaggt tatcaaacag aatcttatct cgggctatct ccggatcctc caggttgatc
     2401 ttttcgactt taagaagcgc aaaatagcgt tcactttctt tgggtgggcg aatctgaccg
     2461 gaaacgatat ccccggttcg gaggttaaaa cgccgaattt gggaaggaga aatatagata
     2521 tcatccggtc cgggcagata gttgtaatcg ggcgccctga ggaaaccaaa gccatccggc
     2581 agagtctcga gaacgccttc cccgtagatc acgccgttct tctcggtctg tgcctgcaaa
     2641 attgcaaaga taagttcctg ctttcgaaga ttgctcgcac cctcgatctt caactccttg
     2701 gcgacctcat tgagttctgc gatgcttttc tccttgagtt cagccaggtt cattgtcgga
     2761 tcttcttttt cagatatcgt cttttctgcc attgtgctgt taagcctcct ttaatagtct
     2821 aaaaaccaaa caacgaggat cacaaacctt tcttgataga gcataccgcc gcccttatgc
     2881 cagtctgttt gtttagccat tataataaaa tctatttata gcgtgtccaa tcgtgatgtg
     2941 attctaccct ggaaggtttt agggtataag ctccgtaagc ttggtatcag taggcagtaa
     3001 gtttcctctg tcccgcttct agaaattcgt gatgtttacg atccacattt cccttaaaga
     3061 ctgtcttgtc gcccgaccga tatgaatgat tcaaatagaa ggagttaagt cgtagagaac
     3121 aattgggttt gtttcattaa agaacttaga cactatggta tatcttcttt attagcatac
     3181 ccaatgggcc tatcgttgtc aaccattttt attttgtccc ataaattatt ccgaaacaga
     3241 tttaacgcaa cggaagcctg catccgggta ggcttcctcc ggaggcgcat agagccggta
     3301 ggaggtccgg taaaaaatgg acaaggcata atggccgggc ccaccccaac ttccacctcg
     3361 taggacctta aatttttgac caaaagcgtc actgtgatag gtgttgccgg gataaggctg
     3421 gtaccaatcc gtggtccatt cccagacatt accgctcata tcgtaaaccc cataggggct
     3481 tttacctgtt tcgaaactcc caacgggtgc aaagtctccc aaaccggcat caccggtatt
     3541 ggcctttttc ccgtcaaagt cgttgcccca cggatactca cgaccatccg tcccgcgtga
     3601 tgccttttcc cactctgcct cggtgggaag acgcttgccc agcca
//
LOCUS       DATMKH010000561         1302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_583099,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000561 DATMKH010000000
VERSION     DATMKH010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583099"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..807
                     /gene="prcA"
                     /locus_tag="VNV63_06690"
     CDS             115..807
                     /gene="prcA"
                     /locus_tag="VNV63_06690"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit alpha"
                     /protein_id="HXC62345.1"
                     /translation="MAYPYYVSPEQMMQDKAEYAKKGIAKGRSIMVMEYVDGILLVAD
                     NPSVSLNKISEVYDAIAFAGAGKYSEFENLRKAGIQHADLKGYVYSREDVTAKSLANA
                     YSQSLGTIFSQEVKPLEVEILVVGVGKTEKENELYRISFDGSIFDERGFTVIGGRSEA
                     IMGFLKNSYKQNLFLKDALKLSVDAFEGANEQKTGPLGPLGLEAAVLDRQRVGRKFQR
                     LTSSEVKQLLKG"
     gene            811..>1302
                     /locus_tag="VNV63_06695"
     CDS             811..>1302
                     /locus_tag="VNV63_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HXC62346.1"
                     /translation="MRNRIFGLESEYGLIFSPNGRVYLPIEKVLGYIFEGLIPDSWPS
                     NAFLMNGARFYQDTGCHPEYSTPECDNIFDLITHDKAGERLLESCLPVAEERLREEGL
                     AGEIFIFKNNTDSMGNTYGCHENYLTRRDVDFWKMTEQLIPFFVTRQIFAGAGKVLKI
                     SGKA"
BASE COUNT          334 a          288 c          373 g          307 t
ORIGIN      
        1 aatttatccc acggtcaaaa ccgtgaccga aaagggaatc agcgatgtga gtgaggaacg
       61 gatgaagggg atttataccg agctcctgga gtcaaaaaag aaagaggcct gatcatggca
      121 tacccttatt acgtttctcc agaacagatg atgcaggaca aggcggagta tgccaagaag
      181 ggcatcgcca agggaagatc cattatggtg atggagtatg tggatggtat cctgcttgtc
      241 gccgataacc caagtgtctc gctgaacaag atttccgagg tctatgatgc gattgcgttt
      301 gcaggggccg gaaagtacag cgagtttgaa aatcttcgaa aggccggtat tcagcatgcg
      361 gatctaaagg ggtatgtgta cagccgggag gatgtgaccg ccaaatcact ggccaatgca
      421 tattctcaga gcctcggcac catctttagc caggaggtca agcccttgga ggtagagatc
      481 ctggtggtgg gtgtgggcaa aaccgaaaaa gagaacgagt tgtaccgtat ctcgtttgat
      541 ggaagtattt ttgatgagag gggttttacc gtgatcggcg gccgttcaga ggcgatcatg
      601 ggatttctaa agaacagtta caagcagaac ctctttctaa aagacgcctt gaagctcagc
      661 gtggacgcct ttgaaggcgc caatgaacaa aaaacaggcc ccttgggtcc cttgggtctc
      721 gaagcggctg tgttggatcg gcaacgggtc ggacgtaaat tccaacgctt gacctcttct
      781 gaggtgaaac aacttctaaa gggttagggg atgagaaaca ggatcttcgg gctggaaagt
      841 gaatatggtt tgatcttctc accaaacggc cgtgtttacc tgccgatcga gaaggtcttg
      901 ggttatattt ttgagggcct catcccggat tcctggcctt cgaacgcttt cctcatgaat
      961 ggggctcggt tttatcaaga taccggatgc catccggaat actccactcc cgaatgcgac
     1021 aatatattcg acctcattac ccatgacaag gcgggtgagc ggctactgga gagctgtctg
     1081 ccggttgcgg aggagcgcct ccgggaggaa ggtctcgcgg gagagatctt catttttaag
     1141 aacaataccg attccatggg aaatacctac ggttgccacg agaattacct tacccgcagg
     1201 gatgtggatt tctggaagat gaccgagcag ctcatccctt tctttgtgac ccgccaaatc
     1261 tttgcgggtg cgggcaaggt tctcaagatc tccggaaagg ct
//
LOCUS       DATMKH010000562         1270 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_583249,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000562 DATMKH010000000
VERSION     DATMKH010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583249"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1243
                     /locus_tag="VNV63_06700"
     CDS             <1..1243
                     /locus_tag="VNV63_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62347.1"
                     /translation="PVSVTVLFPTAKQFPQYLPLIKASQHSFCQVVPPAPPENIHNLI
                     VCDPDTLRQLDILTRVAYSPSYLDEKMKNDWAFMGFLMTLQTEPLKVIDEIKSTVSDE
                     QLEKNMSLILVFLLAHETWHLTHGKGTHFDSDSGEDDPSDTELNEKLMCRNYESFLRQ
                     GFSIYDDRPVALADEGTTDDPAFRQTLKATREIWQEEEDADRFGADTLAKLIYSVSKN
                     GGEDEVYHSSNAIIEDLALMMLQTWFSKMASFAPAHCGAFANQDFYLTLCTCQNKSIY
                     QQVISVFGSTHPPIVLRMYTAAASFLSSLKTNLGIDLVKSKNAAAGPGLYWLLLINGL
                     SDVPLKLSWVQCSDFQRIRSQVTGSIAQELPDLARFRMSGLNRKNPGYPTEDEEVVLM
                     MGKCMSSRTDAPVPGKTGKPN"
BASE COUNT          304 a          334 c          335 g          297 t
ORIGIN      
        1 gccggtttcg gtaactgtcc tgttccccac ggcgaagcaa ttcccgcaat atcttccgct
       61 tatcaaggct tcccagcaca gcttttgcca ggtcgttcct ccggctcctc ctgagaatat
      121 tcacaacctg atcgtctgcg atcccgacac cttgcgtcag ctcgacattc tgactcgagt
      181 tgcatattct ccctcgtatc ttgacgagaa gatgaagaat gactgggcct tcatgggttt
      241 cctgatgacg ctccaaacag agccgttgaa ggttatcgac gagattaagt cgacggtgag
      301 tgatgagcag ctggaaaaga acatgagctt gatcctcgtg ttcctcctgg cgcacgaaac
      361 atggcacctc acccacggga agggaacgca tttcgattcc gactcaggcg aagatgaccc
      421 atcggacact gaattgaatg agaagttgat gtgccggaat tatgaatcgt ttttgcgaca
      481 aggattcagc atttacgacg atcggccagt agccttagcg gacgagggaa caaccgatga
      541 ccctgcgttc cggcagacac tcaaagccac gcgtgaaatt tggcaggaag aggaagatgc
      601 ggaccgtttt ggggcggata ccttggcaaa attgatctac tccgtgagca agaacggagg
      661 agaagatgag gtctaccact cttcaaatgc gatcatcgaa gaccttgccc tgatgatgct
      721 gcagacatgg ttttcgaaga tggcatcctt cgctcccgct cactgcggcg cgtttgccaa
      781 ccaggatttc tatttgacat tgtgcacctg ccagaacaag tccatttacc agcaggtgat
      841 cagtgttttc ggttcgactc acccgcccat cgtgctccgt atgtacaccg cggcggcttc
      901 ttttctgtcg agcctgaaga cgaaccttgg catcgacctg gtgaagtcaa aaaacgcagc
      961 tgcggggccg gggctctact ggttgttact cattaacggg ctaagtgatg ttccactcaa
     1021 actctcctgg gtccaatgtt ctgactttca gcggatccgt tctcaggtta caggcagcat
     1081 tgcgcaagag ttgccggatt tggcccgctt cagaatgtct gggttgaatc gtaaaaatcc
     1141 agggtaccct accgaagacg aggaggtcgt actgatgatg gggaaatgta tgtcaagccg
     1201 caccgacgcg ccggtcccgg gtaaaaccgg gaaaccgaat tgaactttcg ccgggctcga
     1261 agagccaaaa
//
LOCUS       DATMKH010000563         1212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3138839, whole genome shotgun sequence.
ACCESSION   DATMKH010000563 DATMKH010000000
VERSION     DATMKH010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3138839"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1196)
                     /gene="purL"
                     /locus_tag="VNV63_06705"
     CDS             complement(<1..1196)
                     /gene="purL"
                     /locus_tag="VNV63_06705"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HXC62348.1"
                     /translation="MTQPTAPQDIVSQHGLSQEEYQRIRTLLGRAPNPVELGIFGVMW
                     SEHCSYKSSRVHLKKLPTQGERVIEGPGENAGVVDIGGGLVAVFKMESHNHPSFIEPY
                     QGAATGVGGILRDIFTMGARPVALLNSLRFGPLSFPRNRYLFEGVISGIAGYGNCMGV
                     PTVGGEVYFHEIYNRNNLVNVLCLGIAQRDKIVKGRASGVGNPVLYVGSKTGRDGIHG
                     ATMASASFDHESETKRPMVQVGDPFTEKRLLEACLEAIDQDLILGMQDMGAGGLSCSS
                     SEMASKADMGMTIELSSVPLRENGMTPYEIMLSESQERMLLVPKPGREKEVRRIFEKW
                     ELDVATIGTVVSDGLLTVKDQGNVVVQVPAKALTEEAPSYERPLAPPPYLDAIQSLNL
                     DVLKEP"
BASE COUNT          271 a          379 c          295 g          267 t
ORIGIN      
        1 ttcggctctt tgagcacatc gaggttcagc gattgaatgg cgtcaagata cggaggcggg
       61 gccaacgggc gctcataaga gggcgcttct tccgtcaaag ccttggccgg aacctgcaca
      121 accacgtttc cctgatcctt caccgtgagc aggccgtccg atacgacggt cccgatcgtt
      181 gcaacatcca gctcccattt ttcaaagatc cgccggactt ccttctcacg tccgggttta
      241 ggcacaagca acatccgttc ctgagattcc gaaagcatga tctcataggg cgtcattccg
      301 ttttcccgga gcggaacgga ggacagctcg atcgtcatcc ccatgtccgc tttactggcc
      361 atctctgagg atgagcagga aagtccgccc gcgcccatgt cctgcatgcc caggatcagg
      421 tcctgatcga tcgcctcgag acaagcttcc aacagccgct tctcggtaaa ggggtcccct
      481 acctggacca tcggtcgttt cgtttcggat tcatggtcaa aactggccga ggccatcgtc
      541 gccccatgaa tcccgtcccg gccggtcttc gatcccacat acagaaccgg attgccgacg
      601 ccgcttgccc gccccttcac gatcttgtct ctttgagcga ttccgaggca gaggacattg
      661 acgagattat tacggttata aatctcatgg aaataaacct ctcctccaac agtgggcaca
      721 cccatacagt ttccataacc ggcgatgccg gagatcaccc cttcgaagag atagcggttg
      781 cgcggaaagc tgagtggacc aaaacgcaat gaattcagca aagccaccgg tcttgccccc
      841 atcgtgaaga tgtcccggag aatccctccg acaccggtcg cggcaccctg atacggctca
      901 atgaaagagg gatggttgtg actctccatt ttgaaaaccg caacgagccc gccgccaata
      961 tccaccacgc cagcattctc tcccggtcct tcaataaccc gttctccctg agtcggcaac
     1021 ttctttagat gaacccgcga gctcttgtaa ctacaatgct cactccacat cacaccgaag
     1081 atccccaact cgaccggatt gggcgcacga cccaaaagcg ttcggatccg ttggtactcc
     1141 tcttgcgaga gtccatgctg agatacgatg tcctgcggag ctgttggttg ggtcatgatg
     1201 atctcataga tt
//
LOCUS       DATMKH010000564         1339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4433896, whole genome shotgun sequence.
ACCESSION   DATMKH010000564 DATMKH010000000
VERSION     DATMKH010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4433896"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            307..>1339
                     /gene="ltrA"
                     /locus_tag="VNV63_06710"
     CDS             307..>1339
                     /gene="ltrA"
                     /locus_tag="VNV63_06710"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015759379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HXC62349.1"
                     /translation="MNEAKPFCISKKEVWEAYKRVKENKGAAGIDEQTIEDFEKGLKK
                     NLYKIWNRMSSGSYFPPPVRTVKIPKKNGGERKLGIPTVADRIAQQVVKARLEPEVEP
                     LFHADSYGYRPKKSALDAVGQARARCWRYDWIVDLDIKSFFDNLDQKLLLRAVEKHAP
                     ERWVVLYIERWLKAPMQEEDGRLFPRERGTPQGGVVSPLLANLFLHYALDRWLAANHP
                     QVPFERYADDVIAHCRTEKEAQEIRTAIAERLQSCGLELHPEKTKIVYCKDDLRRRRY
                     PNEKFDFLGYTFQPRRSKNRKGKFFINFSPAVSNKAAKAIRDTFRSWKLHQRSDKAIE
                     DLSRMFNPII"
BASE COUNT          369 a          299 c          429 g          242 t
ORIGIN      
        1 aaaccgcggt tgggttacgg ctgcgggaca agctccgacg aaacctgtct acgggcggag
       61 cggcatccgg tatgagaggt ggcctgagct tgccaaggag gcggttaagc ggaacgtggg
      121 aacctaagac tgcgatgcga acggagaagt cacaagtggc agaaccacga gggcgagcgt
      181 acggaagcgc agacttaggg gcggaacagc gcgtagtagc taggaagtcc ccgacaaagg
      241 gatggagcca aggcgctgtg tcaaggagcc tgattcaatg agccaaccag cgatgggagg
      301 agcccgatga atgaggcaaa gccgttttgt atctcgaaga aggaggtctg ggaagcgtac
      361 aagcgggtga aagagaataa gggagctgct gggatcgatg agcagacgat cgaggatttt
      421 gaaaagggac tgaagaagaa tctttacaag atctggaatc ggatgtcatc gggcagctac
      481 tttccgccac cggtgaggac ggtgaagata ccaaagaaaa atggtgggga aagaaagctg
      541 ggaataccca cagtagcgga tcgaatcgcc caacaagtgg taaaggcgag actggagccg
      601 gaagtggaac cgctgtttca tgcagactcc tacgggtatc gaccgaagaa gtcggcactg
      661 gatgcggtgg gccaagcgcg agcgagatgc tggcgctatg actggatcgt agatctggac
      721 atcaaaagct tctttgacaa ccttgaccag aaactgctgt tacgggcagt agagaagcat
      781 gccccggagc ggtgggtcgt tctctatatt gagcgctggt tgaaagcgcc catgcaagaa
      841 gaggacgggc gattatttcc acgggaaaga ggcacccccc aagggggtgt cgtcagccca
      901 ctgctggcta atctgtttct gcattatgca ttggatcggt ggcttgcagc gaaccatcct
      961 caggtgccat tcgagaggta cgctgatgac gtcatcgcgc actgcagaac agagaaggaa
     1021 gcccaggaga taaggacggc catcgctgag cgattgcaga gctgcggact ggaactccat
     1081 ccggagaaga cgaaaatcgt ctattgcaag gacgatcttc gcaggagaag gtacccgaac
     1141 gagaagtttg attttctcgg ttacacgttt cagccgagga gatcaaagaa tcggaagggg
     1201 aagttcttca tcaatttcag tccggcggtt tccaacaagg cagcgaaggc aatccgagac
     1261 acatttcgta gctggaaact gcaccaacgc agcgataaag caatcgagga tctgtcgcgg
     1321 atgttcaatc cgattatcc
//
LOCUS       DATMKH010000565         1207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1857501, whole genome shotgun sequence.
ACCESSION   DATMKH010000565 DATMKH010000000
VERSION     DATMKH010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1857501"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..831
                     /gene="malG"
                     /locus_tag="VNV63_06715"
     CDS             <1..831
                     /gene="malG"
                     /locus_tag="VNV63_06715"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HXC62350.1"
                     /translation="ILVPFVMVISASFRQGNFAPSRLLPDKVSLDHWKFVLGIPYQEV
                     VNPATGQTRTVKADTPPLRWFWNSIKISVLSSAGIIFLSGTAAYAFARLRFKFRSATL
                     SALLILQMFPMVLSLVAFYVILDFTGQYAKWLGLNTQAGLIMIYLGGISNYIWMIKGY
                     FETVPASMEESARIDGASQFQTFVRILLPISLPIFAVVFILSFISYMSEYPVASVVLQ
                     SSENWTLAVGANSFLYEQEKLWGRFAALAVLSGLPITVVFLLCQKFVIGGLTSGGIKE
                     "
     gene            853..>1207
                     /locus_tag="VNV63_06720"
     CDS             853..>1207
                     /locus_tag="VNV63_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXC62351.1"
                     /translation="MSEGSVEIKELVKTYTDDRGRPTFTAVKNISLPIQDGEFMVLVG
                     PSGCGKSTTLRMVAGLEKITSGTISIGGRVVNELEPKDRGIAMVFQNYALYPHMTIFN
                     NMAFGLQLAKKKKDFI"
BASE COUNT          262 a          318 c          304 g          323 t
ORIGIN      
        1 atcctggtgc ctttcgtcat ggtcatctca gcctccttcc gtcagggtaa ttttgcaccc
       61 agccgattgc ttccggacaa ggtgagtttg gatcactgga agttcgtcct gggaattcct
      121 taccaggagg tggtcaatcc agccaccggc caaactcgga ctgtcaaggc cgatacaccc
      181 cctttgcgct ggttctggaa ctcgatcaaa atctctgtgc tctcctccgc cggaatcatt
      241 ttcctttcag gcacggcggc gtatgccttc gcccggctcc ggttcaaatt tagatcggcg
      301 acgttaagcg ctttgctgat cttgcagatg ttccccatgg tgctttcgct ggtagcgttc
      361 tacgtcattc tcgacttcac cggacagtat gcgaaatggc taggtctgaa cactcaggcg
      421 ggtctgatca tgatctacct cggcggcatc tccaactaca tctggatgat caaaggctat
      481 ttcgaaaccg ttccggccag catggaggaa tcggccagaa tcgatggagc ttctcagttc
      541 cagacctttg tccgcatcct ccttccgata agtcttccga tttttgcggt tgtatttatt
      601 ctctcattca tttcctatat gtcagagtac ccagtggcat ccgttgttct tcagtccagc
      661 gaaaactgga ccctggcggt gggtgccaac tcatttttgt acgaacagga aaagctctgg
      721 ggtcggtttg ccgccttggc agtcttgagc ggcttaccga ttacggtggt tttcttgttg
      781 tgccaaaagt ttgtcatcgg cggtctaacc tccggtggca ttaaagaata actaacccca
      841 gatactaatg ccatgagtga aggttccgtt gaaattaagg agttggtaaa gacctacaca
      901 gatgatcggg ggcgcccgac attcactgcg gtcaaaaata ttagcctgcc tattcaggac
      961 ggcgaattta tggtcctggt tgggccatcc ggttgcggca aatcgaccac ccttcggatg
     1021 gttgccggcc tcgagaaaat cacgagcggg acaatttcca tcggcggtcg tgtcgtgaac
     1081 gaacttgagc cgaaagaccg cgggatagcg atggttttcc aaaactatgc gctttatccg
     1141 catatgacga tcttcaacaa catggcgttc ggcctccaat tggcgaagaa gaagaaagac
     1201 ttcatct
//
LOCUS       DATMKH010000566         1397 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3456160, whole genome shotgun sequence.
ACCESSION   DATMKH010000566 DATMKH010000000
VERSION     DATMKH010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3456160"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..416)
                     /locus_tag="VNV63_06725"
     CDS             complement(90..416)
                     /locus_tag="VNV63_06725"
                     /inference="COORDINATES: protein motif:HMM:NF019590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HXC62352.1"
                     /translation="MIYELRHYIPVPGKETAILNRFKNHTFEIFRKKGFKVMNFWIDA
                     KGSGEIWYVLVWESSSSMESSWNTFRSDQEWIEVKGATEKDGPIVERIESTLLIRPEG
                     VAPVNA"
BASE COUNT          339 a          391 c          314 g          353 t
ORIGIN      
        1 tccccttcgg agatttggtc ttctgatttt tgtggaaaaa acgtcttcca acatctttga
       61 accaatcgag aggatcggga gtgacccgat caagcattaa ccggcgcgac accttccggc
      121 cggatcagca gagttgactc gatccgttcg acgatcggtc catctttttc ggtggcccct
      181 ttaacctcaa tccattcttg atcactacgg aaagtgttcc agcttgattc catcgacgaa
      241 gaggattccc aaaccaagac gtaccagatc tctccagacc ccttagcgtc gatccagaag
      301 ttcatcacct taaagccctt tttcctgaag atctcaaatg tatgattctt gaagcgattt
      361 aggattgcag tttccttccc cggtaccggg atatagtggc ggagctcgta aatcatgcag
      421 catcatgttg catccaccct cgaggctaca agcggaatta aaggctccat cgatttgcag
      481 ccaggcgctt tcaatcgttc tcgttttcgt tcttccggta gaatatggag agtgcagtag
      541 agaagcggac gaaaagcgcg atgctcggcg gatgtctctg tggccgattc aggtatcagc
      601 tacggcacga gctactgagc atcaacaatt gccattgcat cgattgcaga cgcgctggct
      661 tcagcagttc tttttcccgc ccttccttca tcattcggga aagagagtca cagcccctca
      721 agatccgcgc tctataccag gaccaacagt gttccggaac gtacgcgaac tcgagcttca
      781 gtcatcaggg tgggtgaaga aaaccgcata tatccaggtt cgtagcactc tgctggcggg
      841 tcgagcaccc tggctccgct ctccaccaac aggctatgtc ccgatccggc aatcaaggca
      901 atcgctagat cgctagtgcg caagttcagt gccaaactac actctgaccg ccaggggata
      961 ttaatctgta tcgcaatcta agaccggttt tggtcatgac cgttcgagcc actatttatc
     1021 cccttgtacc aaccatgttg cgaagggggt gaggagttta tcgaagaagg aatatccttc
     1081 gtcacagcag ctatatcgca ggtcagacct tcacaaattg cgcaaagatc accgaacgta
     1141 atatcacaat ccccgcgttc aatctctctc agatagaccc ttcccaatcc gcatttggcc
     1201 gcaaaaaggt tctgactgta cccgaggaga atccgtgtgc gtcgaatccg aaatccaagt
     1261 cttgaaagtt ccgttttgtt tactgccacg atccgtatcc ccagccgcgt tgtccgcctc
     1321 acatcttcgc acagaccttg cagacagttg ccatactctt tttcgcattt acgcgtctca
     1381 ttaaagtatc gcaatca
//
LOCUS       DATMKH010000567         2310 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3140144, whole genome shotgun sequence.
ACCESSION   DATMKH010000567 DATMKH010000000
VERSION     DATMKH010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3140144"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VNV63_06730"
     CDS             complement(<1..197)
                     /locus_tag="VNV63_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62353.1"
                     /translation="MEEEKQDAQEAPVQIATEPTKPPASLFEALLIAMYTALNSDILR
                     RLSDRFFLTFGLLSIAATLIS"
     gene            complement(262..723)
                     /locus_tag="VNV63_06735"
     CDS             complement(262..723)
                     /locus_tag="VNV63_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62354.1"
                     /translation="MTIYRIEKHVASLLSCPPDLIRFQAAGEPDQAVINAFLAKEKHS
                     RQGRQRDVTLPRYYYLYQPDPRIRLCTVGSTLTGWSGPAWVLIGVNLILGEFHNDDVP
                     GSPFIGNADLRGQQGLQLTMRSVAPFRGFADREFDEPAGFLAELDVSVLEG"
     gene            complement(710..1087)
                     /locus_tag="VNV63_06740"
     CDS             complement(710..1087)
                     /locus_tag="VNV63_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62355.1"
                     /translation="MQVIQVDFRNPEDGAIIGKPQFTKADSHREAVVLALDMFGEDRV
                     TLQEIKLLSPGDRILAGATTAWRYSYQIQVDLGEGEGERYLTALLFATYREKDEMGKP
                     FDTYIQRDDLMVKLAKGENHDDL"
     gene            complement(1131..1685)
                     /locus_tag="VNV63_06745"
     CDS             complement(1131..1685)
                     /locus_tag="VNV63_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62356.1"
                     /translation="MKIEPGSDLERIVFHYGIFSDHLPEEMIAPLREGQPPYRNEAEL
                     FLDEKKYQACLYACYRQSLGEMQRLGYTEQTESIIWPMGRAAALMEIVKPEPDLNPRI
                     SSFPYFAWGDLDKRVVAAAQKYGLLVDEPYRMPDLMWHLLAHRRDGDAVIFLAGDDRS
                     YEHFFGIVWSPERLQRGDIPHAIV"
     gene            complement(1690..2022)
                     /locus_tag="VNV63_06750"
     CDS             complement(1690..2022)
                     /locus_tag="VNV63_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62357.1"
                     /translation="MKLSEKKPAQIEKAEVLAWAWQLMEEQAQWSMNLPPFCACADID
                     APLSGFFHLYYLADPNSFSVFSDKIEYLGREYALPAWAIRFQALFQASDIAQMPSNAA
                     FLTFLQEC"
     gene            complement(2019..>2310)
                     /locus_tag="VNV63_06755"
     CDS             complement(2019..>2310)
                     /locus_tag="VNV63_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62358.1"
                     /translation="IYRDPGSALVRGDAEFTRIMGNRPLPPYYYAYYYFGEAQWRYLM
                     EEMPVIPHAGRRTIATIIERVLPILCSRCGLSPAHKQDGLCRACQKREEEEA"
BASE COUNT          585 a          650 c          541 g          534 t
ORIGIN      
        1 atacttatca atgttgcagc gatagatagc aagccaaagg tcagaaagaa tctatccgac
       61 aatcggcgga gtatatcact attaagcgca gtgtacatcg cgatcaagag cgcttcgaaa
      121 agtgatgctg gcggctttgt cggctcagtt gctatttgta ctggagcttc ttgtgcatcc
      181 tgcttctcct cttccaagac actaacatca agctctgcca gaaacccggc aggctcatcg
      241 ttcttgtgct tcctgcttct cctatccttc caagacacta acatcaagct ctgccagaaa
      301 cccggcaggc tcatcgaatt cccgatcggc aaacccccga aaaggagcga cagaacgcat
      361 agtaagctgc aagccctgct gcccgcgcaa atcggcattc cctataaagg gactgccagg
      421 tacatcatca ttatggaact cccccaggat gagattgacc ccaatcaata cccaggcagg
      481 accggaccat cccgtaagag tgctgccgac tgtacaaagc ctgatgcggg gatcaggctg
      541 atagagatag taatagcgag ggagagttac gtccctctgt cttccctggc gggaatgttt
      601 ttcctttgcc agaaaagcat tgataactgc ctgatccggt tctcctgccg cctggaagcg
      661 aattagatcg ggtgggcagc ttaggagaga tgcaacatgc ttctcgatcc tatagatcgt
      721 catgattctc tcctttcgca agctttacca ttagatcgtc cctctggata taggtatcga
      781 agggcttccc catctcatcc ttttctcgat aggtcgcgaa gaggagcgcg gtaagatagc
      841 gctccccttc cccttcgccc agatcaacct ggatctgata ggaatagcgc caggcagttg
      901 ttgcacccgc aagaatacga tccccaggag acaggagttt tatttcctga agcgttactc
      961 tatcttcgcc gaacatatcg agcgccaata cgactgcttc tctatgcgaa tcggccttcg
     1021 tgaattgggg ttttccgatg atcgccccat cttccggatt cctaaaatcg acttggatta
     1081 cttgcataac tcgacctctt tcttctctaa aatgaatgta ttctccgctg ctaaacaatg
     1141 gcatgcggga tatccccgcg ctggagccgc tctggggacc agacaatacc aaaaaaatgc
     1201 tcatagctcc tatcatcacc ggccagaaaa atgaccgcat ctccatcccg gcgatgcgcc
     1261 aggagatgcc acataagatc aggcatccta tatggctcat ctacgagcag accatatttt
     1321 tgcgcggcag cgaccacgcg cttatccaga tccccccagg caaaataggg gaaactggat
     1381 attctaggat ttagatcggg ctccggcttg acgatctcca tcaaagccgc cgccctgccc
     1441 ataggccaga tgatgctctc agtctgctcg gtatagccca ggcgctgcat ctcgcccagg
     1501 ctctgccgat aacaggcata gagacatgct tgatattttt tctcatcgag aaaaagctcg
     1561 gcctcgttcc ggtaaggcgg ctgtccctcg cgtaagggcg caatcatctc ttcaggcaga
     1621 tgatcagaga agatcccata atggaacacg atgcgctcca gatcggagcc aggttcgatc
     1681 ttcatagaat caacactcct gcaagaaagt cagaaaggcg gcattcgatg gcatctgagc
     1741 aatatcagat gcctggaaaa gtgcctgaaa gcggatggcc caggcgggca aggcatattc
     1801 acgccccagg tattcaattt tatccgagaa tacagaaaaa gaattcggat cagccaggta
     1861 atatagatgg aagaaccccg ataggggagc atcgatatcc gcacaggcgc aaaatggtgg
     1921 aagattcatg ctccattgcg cctgctcctc catcagttgc caggcccagg ccagcacctc
     1981 cgctttctct atctgtgctg gctttttctc actcaacttc atgcttcctc ctcctctctc
     2041 ttctggcatg ctcgacatag cccatcctgc ttatgcgctg gcgataagcc acagcgcgag
     2101 cagagaatgg gcaacacgcg ctcgataata gtagcaattg tccgtcgccc cgcatgcggg
     2161 ataaccggca tctcctccat aagataacgc cattgcgcct caccaaaata atagtaggcg
     2221 taataatacg gtggcaatgg gcgattgccc ataattcggg taaattcggc atcgccacga
     2281 accaaagcgc tccctggatc gcgatagatc
//
LOCUS       DATMKH010000568         5790 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1551370, whole genome shotgun sequence.
ACCESSION   DATMKH010000568 DATMKH010000000
VERSION     DATMKH010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 5790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5790
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1551370"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="VNV63_06760"
     CDS             <1..555
                     /locus_tag="VNV63_06760"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276754.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HXC62359.1"
                     /translation="ERITSTKKGSITSVQAIYVPADDLTDPAPATAFAHLDATTVLSR
                     QLAELGIYPAVDPLDSTSRVLDPRVVGEEHYRVARDVQVVLQRYKDLQDIIAILGMDE
                     LSEDDKLIVARARKIQRFLSQPFFVAEAFTGTPGRYVKLKDTVKGFTEIVEGKHDDLP
                     EQAFYMVGTIEEVIEKAERLGVKR"
     gene            584..889
                     /gene="atpC"
                     /locus_tag="VNV63_06765"
     CDS             584..889
                     /gene="atpC"
                     /locus_tag="VNV63_06765"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HXC62360.1"
                     /translation="MKSTLSLVIATPDELVVKAEVDSVIAPGTEGYFGVLPGHCRFIS
                     TLKAGTVRYRQGGEAQLFEVQGGFADVGPEGVTILAQGLKPLGIVVGVGQAITSRKH"
     gene            complement(947..1621)
                     /locus_tag="VNV63_06770"
     CDS             complement(947..1621)
                     /locus_tag="VNV63_06770"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXC62361.1"
                     /translation="MCTLHSKLNHVGEVEVDAEKIQRVYAIYSGFYDLLFGKIFHQSR
                     IQAVELLGLQPGHRVLEVGVGTGLSLPLYPSYCKVTGIDFSSQMLKKGEAKVARYKLD
                     HVRLHQMDATQMAFSANSFDAVVAAYVISTVPQPHQVISEMLRVCKPGGKIIFLNHFT
                     NGNRLVSSIERTLSPFCKQIGFRTDLDIHSLLAGLPLRIQRFQKVNPLNYWALVQCIN
                     QKKQLA"
     gene            complement(1685..2476)
                     /locus_tag="VNV63_06775"
     CDS             complement(1685..2476)
                     /locus_tag="VNV63_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009945875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HXC62362.1"
                     /translation="MKIFQASDVVHLIDRKERTYTFELKEGARFQHSGEFLSHDDLIG
                     QPDGTIVTLTRGTRLLAVRPTLSEYVMKMPRGAQIIYPKDIALILLWADIYPGASVLE
                     AGIGSGALTLALLRAVGETGQVVSYEVREDFLRRAEKNIALYLGKPPSNLLTRLKDIY
                     EGIEERELDRIALDLPEPWRVVNSAGETLRPGGILLSYLPTILQSSQLTEALHRDGRF
                     TRIETVETLLRPWHIEGMSVRPDHRMVAHTGFITVARRFLPAPAR"
     gene            complement(2650..3420)
                     /locus_tag="VNV63_06780"
     CDS             complement(2650..3420)
                     /locus_tag="VNV63_06780"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HXC62363.1"
                     /translation="MKMTPVEKKRWELWGISLFLLLTSGATVVIFGLITQQSPVKIVF
                     LGIFILLFCAYIIMREFKLQKLQQQLQEEQTKVLEEEIRISSLESRLKELTGLQKAMM
                     AIGMETEPEKALTTILRSALDFFAADRGSIMLVDETSQRLLIAAAIGLKPEYIAKGGP
                     KIGEGIAGHVVQTGEALLLSPQVKTEQYKNFEMKETELRSSICAPLRSRHKIIGVMNY
                     SITNPKKRLFTEYDLKLLTIFAQYAILVIDAAKAARLR"
     gene            3614..4690
                     /locus_tag="VNV63_06785"
     CDS             3614..4690
                     /locus_tag="VNV63_06785"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HXC62364.1"
                     /translation="MLDQTNIIRVITQTISQHFGLPPHEINVKPLCGDASNRTYYRAA
                     LLQENEMISYVVMKLADPEAFKASEEKITTNAIAIQELPYLNVQKHLARCGVAVPKIY
                     FYDEEAGILLLQDLGDELFQNVVRESDRATQERNYRMAIDELLKIQIRGSRLSDGDCI
                     AFGRAFDLPLLMWEFDHFLEYGIDQTNNQPMARQDRDEIRALFLPIAEMLAKEPRYLT
                     HRDYHSRNLMIHQDRVWVLDFQDALMGPCVYDLASLLRDSYINLDEDLIDDLVEYYLQ
                     KKESMDRIRLDRSLFRRVFDLMSIQRNLKAAGRFVYIDVVKKNDRFLQYIPRTIGYVK
                     RNLEKYPELTRLKVLLSRYCRDLV"
     gene            4687..5418
                     /locus_tag="VNV63_06790"
     CDS             4687..5418
                     /locus_tag="VNV63_06790"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HXC62365.1"
                     /translation="MKAIVLAAGFGTRLRPLTNNTPKPLLPVGDRPLIQYNLQLLKKY
                     GITEVAINLHYHGEKIREALGDGRQLGMQILYSEEPEILGTGGGMKKAARFLSDGTFL
                     AINGDILVDINLDKVVEFHRLKKAAATMVLREDPQVDTYGAIELDSEDRIRRFLGRPV
                     WQGKKLSPYMFTGIHVLEPRILEYIPSHGFSPITDAYVAMLKKNEGLFGYLMKGYWRD
                     LGTPESYQQAKTEIKEGTVRLSYAP"
     gene            complement(5430..>5790)
                     /locus_tag="VNV63_06795"
     CDS             complement(5430..>5790)
                     /locus_tag="VNV63_06795"
                     /inference="COORDINATES: protein motif:HMM:NF017913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HXC62366.1"
                     /translation="CAIFISSWVTFFFRLNDGFLNASLGLIHDLLLVLIILEILRTII
                     TYVKIHAVLLEPFLHIGIIAATRRILTAGAQLSGQAVVSKEVFERYLWDTGINALVIV
                     ALGIAVYLFSNRPNRNL"
BASE COUNT         1429 a         1426 c         1575 g         1360 t
ORIGIN      
        1 gagcggatta cctcaacgaa gaagggttcc atcacctcag tccaggcaat ctatgtcccg
       61 gcggatgact tgacggatcc ggcgcctgcc accgcctttg cccacttgga tgcaacgacc
      121 gtgctttccc ggcagttggc cgagctgggg atttatccag cggtagatcc gttagattct
      181 acctcacgtg ttctggatcc ccgtgtggtt ggagaagagc attaccgtgt ggcccgggat
      241 gttcaggtgg tcctccaacg ttacaaggac ctccaagaca tcattgcgat cctgggaatg
      301 gatgagctgt ccgaggacga caaactcatc gtggcccgtg ccaggaagat tcaacggttc
      361 ctttctcaac cctttttcgt ggccgaggcc tttaccggaa caccgggacg ttacgtcaaa
      421 ctcaaggaca ccgtgaaggg tttcacggag attgtggaag ggaagcatga cgacctgcca
      481 gagcaggcct tctatatggt tggtacgatt gaggaagtaa tcgagaaggc ggagcgtctg
      541 ggggtaaaac ggtaagcatc cgagactgtc gagcaagaga ccaatgaaat ctaccctatc
      601 tttagtgatc gcgacaccgg atgaactggt tgttaaggcc gaagtggact cagtgatcgc
      661 gccgggaacc gaaggctact ttggagtctt gccgggtcac tgccgcttca tcagcacact
      721 caaggccggc actgttcgat atcggcaagg cggagaagct caattgtttg aagtgcaagg
      781 cggttttgcg gatgtggggc cagagggagt aacgattctg gcgcaggggc ttaaacctct
      841 cggaattgtt gttggagtag gccaagcgat cacctcgcga aagcactaat ctaatatagg
      901 ctgccgggcg aaggttccga agaagcaagt ataggttagc aatcaatcaa gcgagctgtt
      961 ttttctgatt gatgcactga accaaggccc aataattcaa agggttgacc ttttgaaatc
     1021 tctgaattct taaaggaaga ccggcgagaa gtgagtggat gtccaaatcg gtccgaaaac
     1081 cgatttgctt gcagaatgga gacaaggttc gctcgatact tgagacgaga cggttcccat
     1141 ttgtgaaatg atttaggaag atgattttcc cccccggttt gcaaacacga agcatttcac
     1201 tgatgacttg gtgaggttga ggaaccgtac tgatgacata ggctgccacg accgcatcaa
     1261 agctatttgc tgaaaaggcc atctgggttg cgtccatttg atgaagcctg acatgatcaa
     1321 gtttgtatcg ggccaccttg gcctcgcctt ttttaagcat ctgactggag aagtcaattc
     1381 cagtaacctt acagtaagat ggataaagcg gcagcgacag tccggtcccc acaccgactt
     1441 ccaggacccg atgtcctggc tggagtccta agagctcgac cgcctgtatc cgggattgat
     1501 gaaagatctt cccaaagagc agatcgtaga accctgaata gatggcgtaa acgcgctgga
     1561 ttttttcggc atccacctca acttctccta catgattcaa ttttgaatga agtgtacaca
     1621 ttttagcaga ttgtcccaaa gaatcaagat aaatcctcgc gtcttggcct caatcggata
     1681 gagtttaacg cgctggagcc gggagaaacc ggcgggccac agtgataaag ccggtatgag
     1741 ccaccatacg gtgatccgga cggacgctca tgccctcaat gtgccagggt cggaggagcg
     1801 tctcgaccgt ttcaatccgc gtgaagcggc cgtcacggtg aagcgcctcg gtgagctgac
     1861 tcgactgcag gatcgtgggg agataagaaa gcaaaatacc gcccggccga agcgtctcgc
     1921 cggctgagtt cacgacgcgc cagggctcgg gcaggtctag agctatccgg tccagttccc
     1981 gctcttcaat gccttcatag atatccttca atcgtgtcag aagattcgac ggcggtttgc
     2041 ctagatacaa cgcgatattt ttctcggcgc gccgaaggaa atcctcgcgg acctcatacg
     2101 agacgacctg gcctgtctct cccacggccc gcagaagcgc cagggtcaat gcgccggaac
     2161 cgataccggc ctccaacacg gaagctcccg gatagatatc ggcccagaga aggatcaggg
     2221 cgatgtcctt ggggtatatg atctgtgcac ctctgggcat tttcataaca tattccgaga
     2281 gagtcggtcg aactgcgagc aatcgcgtgc cccgcgtcag tgttacgatc gtgccatccg
     2341 gctggccgat caagtcatca tgggatagaa actctccgct gtgctggaag cgggctcctt
     2401 cttttaactc aaaagtgtag gttcgttcct tccgatcgat cagatgaacc acatcgcttg
     2461 cctgaaatat tttcatactc taacgagcct cggtgtgtga catggcctgg atcatagatg
     2521 aattcataag agatttcaac aaatcggttt tattcctgat gctgcgtcgt tcaagattta
     2581 aaagacggag aaagaaggcg gacatcccaa gatctagggg ttgtctcgtg tgtgccgcgc
     2641 tcgcagtctc tatcttaaac gcgcggcctt tgcggcatcg atgactagta tggcatactg
     2701 ggcaaaaatc gttaggagtt tgagatcata ttcggtaaaa agacgtttct ttggattcgt
     2761 gatgctgtaa ttcatgacac cgatgatctt gtgcctgctt ctcaacgggg cacagatgct
     2821 tgagcgaagt tcggtttctt tcatctcaaa gttcttgtac tgctctgtct taacctgagg
     2881 ggagaggagg agggcttcgc ctgtctgaac gacatgccca gcgattcctt ccccgatctt
     2941 ggggccgcct ttggcgatat actcgggttt gaggccgatg gctgcggcga taaggagcct
     3001 ctggctggtc tcatccacca gcatgatgga gcctcgatcc gccgcaaaaa agtccaatgc
     3061 agaccgcagg attgtagtaa gagccttctc gggttctgtc tccatcccga tcgccatcat
     3121 cgctttttgc agcccggtca attccttgag acgcgactca agactggaga tcctgatttc
     3181 ttcctccaac accttcgtct gttcttcctg gagttgttgc tggagtttct gaagcttgaa
     3241 ttccctcatg atgatgtagg cgcagaagag cagaatgaag ataccgagga agacgatctt
     3301 gaccggggac tgctgtgtga tcagaccgaa gatgacgacg gttgcgccgg aggttagaag
     3361 caggaaaagt gagattcccc ataattccca gcgttttttc tcaacagggg tcatcttcat
     3421 aggcaggtca cggaatgacg agcacgatcc attgacaagt cgtttcttag aagattataa
     3481 cgagtttatt caaatcaatc aacacctttt taatactgcg gccggggcag attgctccta
     3541 cagggttcag aactgatctt gattgacaca ttgacagacc acaaatttaa tggcagaata
     3601 atttcggatc agaatgttgg accagacgaa tattattcga gtcatcacac aaaccatcag
     3661 ccagcacttt gggttgccgc cccatgagat caacgttaag cccctgtgcg gagacgcctc
     3721 caacagaacc tactatcggg cggcgcttct ccaagaaaac gagatgataa gctatgtggt
     3781 gatgaagctg gccgacccgg aggcctttaa ggcctctgaa gaaaagatta cgaccaatgc
     3841 gatagcgatc caggaactcc cttatcttaa cgttcagaaa caccttgccc gatgcggcgt
     3901 ggcagtccca aagatttatt tttacgatga agaggcaggc atcctcctcc ttcaagacct
     3961 cggcgatgag ctatttcaaa acgtagtacg tgaatcggat cgggcgacac aggagcgaaa
     4021 ctaccgcatg gcgattgatg aactcctcaa gatccagatt cgcgggagcc ggttgtctga
     4081 tggagactgc atcgctttcg gccgggcgtt tgatctcccc ctgctgatgt gggagttcga
     4141 tcacttctta gaatacggaa tcgatcagac caacaatcaa ccgatggcgc gacaggaccg
     4201 tgacgagatc cgggcgcttt ttcttccgat tgcagaaatg ctggcgaagg agccgagata
     4261 tctgacccat cgcgattacc acagtcggaa ccttatgatc caccaggacc gggtctgggt
     4321 cttggacttt caggatgcct tgatggggcc ctgtgtctat gatcttgcct ctctgttgcg
     4381 ggattcgtat atcaatctgg atgaggatct gattgatgat ctggtggaat actatctcca
     4441 aaagaaagag tccatggatc gaatcagact ggataggtct ttattccggc gggttttcga
     4501 tctcatgagc atccagcgta accttaaagc agcaggccgt tttgtttata tcgatgtcgt
     4561 taaaaaaaac gatcggtttt tgcaatacat tcctcggacg atcggctatg tcaaacggaa
     4621 ccttgaaaaa tacccggagc tcaccaggct gaaagtcttg cttagccgct actgtcggga
     4681 cctcgtatga aggccatcgt cctcgctgcc ggtttcggaa cacgtctccg tcctttgacc
     4741 aataacacgc ccaagccgct tctgcccgtg ggcgacaggc ccctgatcca gtacaacctg
     4801 cagctcttaa aaaaatatgg catcacggaa gtagcaatca accttcatta tcatggggag
     4861 aagatccggg aggcgctggg ggatgggcgg cagctgggca tgcagatact ctattcagaa
     4921 gagccggaga tcctgggaac cgggggcgga atgaagaagg ctgctcgatt tctgtccgat
     4981 ggaacctttc tggcgatcaa cggggatatc ttggtggata tcaacctgga caaggtggtg
     5041 gagtttcatc gcttgaaaaa ggcggcggcc acaatggtcc tgcgcgagga tccgcaagtt
     5101 gatacttacg gggcaatcga gctcgattcc gaggaccgaa tccgtcgttt tctgggccgg
     5161 ccggtctggc agggaaagaa actctcgcct tatatgttta caggtattca tgttctggag
     5221 ccgcggatac tggagtacat tccatctcat ggtttctctc cgatcacaga tgcctatgtg
     5281 gcaatgctga aaaaaaacga ggggctgttc ggctatctca tgaaaggcta ctggcgtgac
     5341 ctgggaacac ccgaaagcta tcagcaggcg aagaccgaga tcaaagaggg aacggtgcgg
     5401 ttgagctatg ccccctgaga tgagagtttt tacaagttgc gattcggcct gtttgaaaat
     5461 aagtagacag cgatccccaa agcaacgatc acgagggcat tgatcccggt gtcccagagg
     5521 tagcgctcaa agacttcttt gctcaccact gcctggcctg agagttgagc gcctgcggtg
     5581 agtatccgcc gcgtggcggc gatgatgccg atgtgaagga atggctccag aagaactgca
     5641 tggatcttca cataggtaat gatcgtccgc aggatctcca gtatgatgag aaccagaagc
     5701 aggtcgtgga taagaccgag agaggcattg aggaatccat cgttgagccg gaaaaagaac
     5761 gtcacccagc tagagatgaa gatggcgcat
//
LOCUS       DATMKH010000569         1417 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1859832, whole genome shotgun sequence.
ACCESSION   DATMKH010000569 DATMKH010000000
VERSION     DATMKH010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1859832"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(321..1127)
                     /locus_tag="VNV63_06800"
     CDS             complement(321..1127)
                     /locus_tag="VNV63_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011505186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HXC62367.1"
                     /translation="MKKLIVFDLDGTLAESKSPLDAEMAVLLHALLGTAQVAVISGGD
                     WPQFEKQLLSNLPQDELLANLSLLPTCGTKFFQYTGTWKKIYSEDFTVDEKEKIITAL
                     KQALATAGFKVEKLWGETIEDRGSQITYSALGQQAPLEEKNKWDPDFSKRKAIKAILD
                     PLIPGFSVRIGGATSIDVTKSGIDKAYGIRKLRDLLGISLQDMIFIGDALFPGGNDFP
                     VRATGVVCISVHGPMETKPVTEAIVACLDQFPHPEPAEADSMRVFGFAAH"
BASE COUNT          322 a          369 c          391 g          335 t
ORIGIN      
        1 catggtttcg gcaagacgcg gcccatcgcc tcgaacgaca cttccgaagg ccgccagcag
       61 aatcgccgag tggaattgat cgtctccggc gaagtgatcg gcaccacgat cggtacgatt
      121 gtgctgcagc cgatcgcgac atcaacgtct ccgcagtaat cctaccctga ccagaagggc
      181 cggctcagat cctgagtcgg cccttttttt gtttccggcg gtttttctcc gggctgacta
      241 aggcgaatct gttgagtgga agtgagcggg gatggcgctg tcgagcaagc agggagaaga
      301 accggcgctg ctcattgaga ttagtgcgcg gcaaatccaa acacgcgcat cgagtccgcc
      361 tcggcaggtt ccggatgcgg aaattgatcc aaacaagcga cgatggcttc agtcaccggt
      421 ttcgtctcca tgggcccgtg aacagagata caaactacgc ccgttgctct gaccggaaag
      481 tcatttcctc ccgggaacag ggcatcccca atgaagatca tgtcctgcaa ggaaattccg
      541 agaaggtctc ggagctttcg gattccgtag gccttgtcaa taccggattt ggtaacatca
      601 atggaagtcg cgccgccgat tcggacagaa aaccccggga tcaatggatc gaggatcgcc
      661 ttgatcgctt tccgtttgct gaaatcggga tcccacttgt tcttttcttc caagggggcc
      721 tgctgaccta gtgctgagta tgttatctgg ctgccccggt cttcaatggt ttctccccac
      781 agcttttcga ctttgaaacc ggctgttgcg agcgcctgct tgagagcggt gatgattttc
      841 tctttttcat ccacggtgaa gtcttctgaa taaatctttt tccaagtccc cgtgtattgg
      901 aagaattttg ttccacaggt tggaagaaga gaaagatttg ccagaagttc gtcctggggc
      961 aaattggaga gaagttgttt ttcaaattgc ggccaatcgc ctcccgaaat cacggcgact
     1021 tgagcagtgc cgaggagagc atggagcagg actgccattt ccgcatccaa aggggattta
     1081 ctttcggcaa gcgtgccgtc cagatcaaac acaatcagct ttttcactat tgttccgcct
     1141 tcgagttcct gcaaagcaag agtttccacc ccgtcaggac gtggacggga acgagccgat
     1201 ctcgcgccga gaaagaagga acggcactat cactggctgc ttctttttcc cgcatcaaga
     1261 attcggggga aggtcagtgc aggatggaca gtccctgctt tcagcgcgat ttcactcaga
     1321 cggcgatggt aactccgagg gaatggcaac gctgttccta aagacacact gggttggaat
     1381 gagaagcgat tgggttcgca actggacgat gtgactc
//
LOCUS       DATMKH010000570         1243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3776616, whole genome shotgun sequence.
ACCESSION   DATMKH010000570 DATMKH010000000
VERSION     DATMKH010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3776616"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..>1243
                     /locus_tag="VNV63_06805"
     CDS             70..>1243
                     /locus_tag="VNV63_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62368.1"
                     /translation="MNFDLDAFISYAHMDNVGLIQGHQGWISNLHRALEVKVGQLLGK
                     QPHIWRDPKLQGNDVFADTLLERLRHVAVLISVLSPCYVNSEWTRRELVEFWKAAEAQ
                     GGVRLHDKARIFKVLKTPVRLEMHPPELQPLLGYEFFQIDPDTGKMHELDEAFGSEAQ
                     IAFWMKLDDLAQDICSLLELLGAAGITDSSGEKSTRETIFLAETTIDVKEQREMIRRD
                     LQQHGYTVLPAGGLPLVGSEIVSSFREDLARSRMSIHLVGKNYGTIPEGSTESIPEIQ
                     DELAIDRVNQGNFSRLIWISPGLQVDDSRQQKFIEHLRMDPRIQKGSDLLETPLEDLR
                     TVIQDRLKQAAEPVRKKSVTPTSKQHPAQVYLIYDQRDVGVTSPWADLLFDRGFEVL"
BASE COUNT          304 a          312 c          352 g          275 t
ORIGIN      
        1 gcacttctcc agtttcctcg cctgggaccg atacattcag ggctgtgcat caattgtgag
       61 agggacgcca tgaatttcga tcttgacgcc tttatcagct acgcacacat ggacaatgtc
      121 gggctcatcc agggccacca aggatggatc tcgaacctcc accgtgcgct agaagtcaag
      181 gtggggcaac ttctgggcaa acagccgcac atctggaggg atcccaaact gcaaggaaac
      241 gatgtattcg cggataccct gctggagcgc ctgcgccacg ttgctgtcct catttcggtt
      301 ctctcgcctt gctatgttaa ctcggagtgg acccgcagag agttggtcga gttttggaag
      361 gccgcagaag cgcaaggagg ggttcggctg catgacaagg cgcgaatttt caaggtgctg
      421 aagacacctg tccggctgga gatgcatcca ccagagttgc aaccgctgtt ggggtacgag
      481 ttcttccaga tcgacccaga tacggggaaa atgcatgaat tggacgaggc cttcggatcc
      541 gaagcccaga ttgctttctg gatgaagcta gacgatctgg cgcaagatat ttgttcccta
      601 ctggagttgc ttggagctgc tggaataaca gactcgtcgg gggagaaaag cacacgagag
      661 acgatctttc tcgcggagac cacgatcgac gttaaagaac agcgcgaaat gatcaggcgg
      721 gatttgcaac agcatggcta tactgtgctt ccagcaggtg gattgccact agtcgggtca
      781 gagatcgtgt cttcgttccg cgaggatctg gcgcgaagcc ggatgtcgat tcatctggta
      841 ggtaagaact acggtaccat tcccgaggga agtacggaat cgattcccga gatccaggac
      901 gaactggcaa ttgaccgggt caatcagggc aacttttccc gcctcatatg gatttcgccc
      961 ggtttacagg ttgacgatag ccggcaacaa aaattcattg agcacttgcg gatggatccc
     1021 aggattcaaa agggatcgga cctgctggaa actccgctgg aagacctgag aacggtcatt
     1081 caggatcgcc taaagcaagc cgcagaaccg gtccgcaaga aaagcgttac gccaacttca
     1141 aaacaacatc ctgcgcaagt ctaccttatt tacgatcagc gggatgtagg tgtgacctcg
     1201 ccttgggcag atctcttgtt tgatcgcgga ttcgaagttt tac
//
LOCUS       DATMKH010000571         1086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4731512, whole genome shotgun sequence.
ACCESSION   DATMKH010000571 DATMKH010000000
VERSION     DATMKH010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1086
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4731512"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..837)
                     /locus_tag="VNV63_06810"
     CDS             complement(7..837)
                     /locus_tag="VNV63_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HXC62369.1"
                     /translation="MAGEDLDTGIKIYLREIGQIPLLTPQEEIDLAARIKIGDRDARA
                     LMIKANLRLVVKIAHDYANFGLPLLDLVSEGNIGLMKAVERFDPAKGGKLSTYAAWWI
                     KQSIKRALANQSKTIRLPVHLVDKISKIRRVSVQISEELGREPTDDELAEEIGISTRK
                     VSQLKTVSIRPASLDAPISDDDSTEFGEIVGDEDAQTPFELLRDKNLRDEVSDLLEIL
                     DDRERKIIFQRFGLDGGKPKTLEEVGKKFRVTRERIRQLQNTALAKMRRALSKKETPT
                     "
     gene            complement(819..>1086)
                     /locus_tag="VNV63_06815"
     CDS             complement(819..>1086)
                     /locus_tag="VNV63_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62370.1"
                     /translation="LEKLGSRMIELPLMTDPHPPRDDGRSGKHPACIPKGKEPLPSRP
                     EQLATSSTESIRIVLENDSTDLDWVVASRDNFNTNAETVWLVKI"
BASE COUNT          233 a          309 c          258 g          286 t
ORIGIN      
        1 gctctcctag gtcggcgtct ctttcttgct cagcgcccga cgcattttcg ccagggcggt
       61 attctgaagc tgacggattc tttctcgggt aaccctaaac ttcttgccca cttcttcgag
      121 ggtcttcggt ttcccaccat ctaatccgaa ccgctggaag attattttac gctcccggtc
      181 gtcgaggatc tcaagcaaat cgctcacctc gtcgcgcagg ttcttgtctc tgagcaactc
      241 gaacggcgtt tgcgcatctt catccccaac aatttcacca aattcagtcg aatcgtcatc
      301 gctgattggg gcgtccaacg aagcgggccg aatcgagacc gtctttaatt gagagacttt
      361 gcgagtggag ataccgatct cttccgccaa ctcgtcatca gtcggttccc ggcccagttc
      421 ttcacttatc tgcacggaaa cccggcggat tttggaaatc ttatccacca agtgaaccgg
      481 aaggcgaatg gttttactct gattggccaa agctcgtttg atcgactgtt tgatccacca
      541 ggccgcgtag gtgctcaatt ttccgccttt tgccggatcg aatcgctcga ctgccttcat
      601 caatccgatg ttgccttccg atactaaatc caacaaaggc aaaccgaaat tcgcataatc
      661 gtgagcaatc ttaacgacca gacggagatt ggccttgatc atcagcgctc gcgcgtctcg
      721 gtctcctatt ttaatccgcg cggccagatc aatctcttct tgcggggtta gcagcgggat
      781 ctgcccgatc tcccgaaggt aaattttaat gcccgtatct aaatcttcac cagccataca
      841 gtttcagcgt tagtattgaa gttgtcacgg gacgctacca cccagtcaag atccgtgcta
      901 tcgttctcaa gaacgattct tattgactcg gtagagctag ttgccaactg ctccgggcga
      961 gagggtagag gctcctttcc cttaggaatg caggcgggat gtttaccaga acgcccatcg
     1021 tcgcgggggg gatgcggatc ggtcatcaaa ggcagctcga tcatgcgact ccccagtttt
     1081 tccaga
//
LOCUS       DATMKH010000572         1144 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1553549, whole genome shotgun sequence.
ACCESSION   DATMKH010000572 DATMKH010000000
VERSION     DATMKH010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1553549"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..275)
                     /locus_tag="VNV63_06820"
     CDS             complement(3..275)
                     /locus_tag="VNV63_06820"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HXC62371.1"
                     /translation="MAFPKSQKSILSLKSLLFTPGTKSDRFDRAAGVHADALIIDLED
                     AVAPSAKKEAREASLRYLEALSISLPCVLRINAPDTRVGLDDLHAL"
     gene            complement(910..>1144)
                     /locus_tag="VNV63_06825"
     CDS             complement(910..>1144)
                     /locus_tag="VNV63_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62372.1"
                     /translation="RRMPGIFGPGVAGNGLRRAEEVNPRRRAKNGLGLAAEQGPILSD
                     GTLGVNRRVCLAHGQIGTALRRGRAQAQTARSP"
BASE COUNT          271 a          306 c          295 g          272 t
ORIGIN      
        1 atttaaagcg cgtgcaagtc atcgagccca actctggtat ccggagcatt gattcgaaga
       61 acacacggaa gacttatgga aagcgcttcg agataccgaa gggaggcctc tctagcctct
      121 ttcttcgcgg acggtgccac cgcgtcttcg agatcaataa tcagtgcatc ggcatgaacc
      181 ccggcggctc gatcaaagcg atcggattta gttccgggcg tgaaaagcaa acttttcaag
      241 gaaagaattg atttctgtga tttgggaaag gccattggtg ctccttcgag gtctctttag
      301 gttgttctga catgcttgct gctgatttaa ggttgatgta agcattcggg ctcagcttcc
      361 ccttcgtgaa ctcctgtccg actcaatcaa ccacgagcct ccgggtggct ggctcacacc
      421 aacgccaatt gcacgcagat cccctttttc gccgtcatta tttgacagat cgaacatttg
      481 agcggggggt cgtaagtcac caaagattaa ttcatctctt cgacaggatc aatgctacac
      541 atacacagaa ttcagaacgg cgttctcgaa gatgcgagaa gcgtcggcat cggacaggag
      601 gaccggaagt tctccaattc tatgagattg agctgtgctc cgccgcctgg gtttttcaga
      661 cccaacgaat tcgatatagg agcgttcggc gcccagggtc cttgccggtc gatcgataga
      721 ccccatgaag tgaggtaggt aatcatctga cgcggaatag atgggatgta cgggttgagt
      781 aagcccccaa aattggtcga ctaaacaaga gccaatttta agagccgcag aattgccgaa
      841 agttcctcga gcatagaagg ttgcgaaaaa tcctttgaga cttttggtac ctttgttgga
      901 atttaacctt caaggggatc tggcggtctg agcttgcgct cgaccacggc gcagcgctgt
      961 cccgatttgg ccatgtgcca ggcaaaccct ccgattcacc ccgagcgtac catccgatag
     1021 aatcggaccc tgttcagcgg ctaagcccaa tccgttttta gccctgcggc gcggattgac
     1081 ctcttccgcc cgccgcaaac cgtttcctgc gacccccgga ccgaaaatcc ccggcattct
     1141 ccgg
//
LOCUS       DATMKH010000573         1315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1230105, whole genome shotgun sequence.
ACCESSION   DATMKH010000573 DATMKH010000000
VERSION     DATMKH010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1315
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1230105"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..604)
                     /locus_tag="VNV63_06830"
     CDS             complement(134..604)
                     /locus_tag="VNV63_06830"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXC62373.1"
                     /translation="MDAMVDDRVMFRQESLSDFREECEQIIEAHYQEIALDKNAQKLD
                     PDWEEYERLEKLGRFWLMTARHRGMLVGYIVMLITKHLHYKSLVMAVEDIHYLSPEYR
                     RGLLGYKLLAIADREMKKLGVKKCVFRTKFFKNHGKLFERLGYKPEDEVWGKVF"
     gene            complement(607..1182)
                     /locus_tag="VNV63_06835"
     CDS             complement(607..1182)
                     /locus_tag="VNV63_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62374.1"
                     /translation="MAEAALKIEPEYHPSRRVARYDLVEMGAYLCKRLREKEHYPHLQ
                     DRQILGFLQGCSDGPSSGEYLFIRNDRGFLLAQSYRDFFEPCPWVKELFCFAYYEDAR
                     YAPDGQTAEDYQKEVKASAIIQAAALYEDLIRWAQKIGANEIIVDKFTDVPVKNDKDP
                     TDMKTIKGRMGKLFKSEEIFARLDPDKIRKV"
BASE COUNT          290 a          382 c          300 g          343 t
ORIGIN      
        1 caccaatccc ggcggcggga gctgcggcgg cctcaccaaa gccaagcgcc gatcccagac
       61 cgctaagaat atccccgata ccgagcgcat ctccgagagc ggctattccg gtggtgatgc
      121 ccatgcaatc ctcctaaaac accttacccc agacttcatc ctctggctta tatccaagtc
      181 tttcgaaaag cttaccatga tttttgaaga attttgttcg aaacacgcat ttcttgaccc
      241 ccagcttctt catttcccga tcagcgatcg ccaatagctt gtatcccaac aagccccttc
      301 ggtattcagg gctcagatag tgaatgtcct ctaccgccat gacgaggctc ttgtagtgca
      361 gatgcttggt gatcagcatc acgatatagc cgaccagcat gcccctgtgg cgtgccgtca
      421 tgagccagaa tcgacctagt ttctcaagac gttcgtactc ctcccaatcc gggtctagct
      481 tctgggcatt cttgtccagt gcgatctcct ggtaatgcgc ttcgatgatc tgttcacatt
      541 cctctcggaa gtcagaaaga ctttcctgcc ggaacatgac cctgtcatcg accatcgcat
      601 ccatgtctag accttcctga tcttgtcggg gtcgagtcgg gcgaagattt cctccgactt
      661 gaacagtttc cccatccggc ccttgatggt tttcatgtcg gtggggtctt tgtcgttctt
      721 caccggaacg tccgtgaact tatcgacgat gatctcgttg gcaccgatct tctgagccca
      781 tcgaatcaga tcttcataaa gcgcagcggc ttgaataatg gccgaggcct ttacctcttt
      841 ctggtagtcc tccgctgtct gcccatcagg tgcgtatctc gcatcctcgt aataggcgaa
      901 gcagaacagt tccttcaccc aagggcacgg ttcaaagaaa tctcggtagg attgagcgag
      961 caggaaacct cgatcattcc tgataaacag gtactcacca gacgatggac catccgagca
     1021 accctgaagg aatccgagga tttgccggtc ctgaagatga ggatagtgtt ctttctcccg
     1081 gagcctcttg cacagatacg cccccatctc gacgaggtcg tatctcgcga cacggcggga
     1141 tggatgatat tccggttcga tcttgagtgc ggcttcggcc atggtttatc ctatttccag
     1201 tgtttccgat gcttgaagat gctcgatgaa attattgtac acattccccg caatcgtact
     1261 cttgttcttc caatcccaat catcgagatt gaagcctgat atccccaaaa tctga
//
LOCUS       DATMKH010000574         3701 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4438609, whole genome shotgun sequence.
ACCESSION   DATMKH010000574 DATMKH010000000
VERSION     DATMKH010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3701
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4438609"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /locus_tag="VNV63_06840"
     CDS             <1..861
                     /locus_tag="VNV63_06840"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXC62375.1"
                     /translation="KLEKHLEIIDREIQVAEKIINDLTDIVKPVDSVFASTDLQQHLI
                     QSERLAEIGIMSAGIAHEVNNPLSVMLGKAEMILEEEDPVRIKKYAQDIIKYSKKASD
                     IVKGITFYSRAASSPGSRINLNDQLKEAIKISKYSINFDNVELLTDCQEIPLISGNAG
                     EIQQVFVNLLNNAVEAMEGSGRLFVTSRSEDGSVIITIKDTGPGIKKEHLNKLFTPFF
                     TTKDPGKGTGLGLNIVHKIITNHRGSISAESEEEKGATFVVRFPIAQESPEGEDKQVK
                     TNMYDQEEGI"
     gene            861..1262
                     /locus_tag="VNV63_06845"
     CDS             861..1262
                     /locus_tag="VNV63_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXC62376.1"
                     /translation="MSTILVIDDEEDIRSIVKHHLVKAGYKVVEAEDGEQAIQKINEG
                     DNPLTIDAAICDIRMPKINGVEAISYFRKEYPSVPVIVITGFPDTNLAVSLLKKGVKD
                     YIVKPVEKEKLLAAVKKAVSEKAEADGGLMA"
     gene            1671..2849
                     /gene="alaC"
                     /locus_tag="VNV63_06850"
     CDS             1671..2849
                     /gene="alaC"
                     /locus_tag="VNV63_06850"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HXC62377.1"
                     /translation="MKEFHRIKQLPPYVLSVVTAAKLEARRRGEDIIDFGMGNPDEPT
                     PPHIVEKLIEAAKKAQNHRYSASRGITKLRLAIADWYKRNYDVKLDPETEAIVTIGSK
                     EGISHLALAVLGPGDSVLVPNPTYPIHTYSVIIAGGQVLDVPLRPETNFFNELVKAYE
                     QAKQKPTMMILNFPHNPTTAIVDIGFFKKIVEFAKAHDLMVVHDLAYADLAFDGYKAP
                     SFLQVPGAKEVGVEFFTLSKSYNMPGWRVGFCVGNREIIGALTKLKSYLDYGMFQPIQ
                     IASIIALNGPQDCVRETVETYQRRRDVLVDGLNRIEWKVDKPLATMFVWARIPEEFRA
                     MGSLEFSKLLLSKAKVAVSPGIGFGKYGDDYVRFALVENEHRTRQAIQGIKKILKGKR
                     "
     gene            2846..>3701
                     /locus_tag="VNV63_06855"
     CDS             2846..>3701
                     /locus_tag="VNV63_06855"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HXC62378.1"
                     /translation="MKARHQRSVIKVGLIGLGTIGTGVAKLIQSNQDLIRRRLGCGLE
                     LSKVADKDIKRDRGVSLPKGVLTTKVQDLIDDPEIEIVVELMGGYDPAKKFILSALAH
                     RKHVVTANKALLAVHGEQIFEAAEQAGCDLGFEGSVGGGIPIIRAVKEGLAANKILSI
                     YGIINGTSNYILTQMTENKRSFKEVLTEAQKKGYAEADPSFDVEGIDSAHKLAILVTL
                     AFGTPVTFKEIYTEGITQITPLDIEYARELGYQIKLLAIAKLNQKEKDHTAIEARVHP
                     TMIPENYLI"
BASE COUNT          963 a          886 c         1027 g          825 t
ORIGIN      
        1 aagttagaaa aacatctcga gatcatagat cgagagatcc aggttgcaga aaagatcatc
       61 aacgacctga ctgacattgt gaagcccgta gactcggtct ttgcttcaac tgatcttcaa
      121 caacatctga tccagtcgga gaggttggcc gaaatcggga tcatgtcggc cgggatcgcc
      181 catgaagtga ataatcccct ttctgtcatg ctcggcaagg ccgaaatgat cctggaagaa
      241 gaggacccgg ttcgcataaa gaaatatgcc caagacatca tcaaatattc caagaaggca
      301 tcggatattg tcaaaggcat taccttctat tcacgagccg cttcatcacc gggaagccgg
      361 atcaatctta acgatcagct caaggaggcg atcaagatct ctaaatactc aatcaacttt
      421 gacaacgttg agttattgac cgactgccag gagattcctc tcattagcgg taatgccgga
      481 gagatacaac aggtttttgt gaatttgctg aacaacgcgg ttgaagccat ggaaggaagc
      541 ggccgcctgt tcgtgacgtc gcgatctgaa gacggctccg ttataatcac cattaaagac
      601 acaggacccg ggatcaaaaa agaacatctc aacaagcttt ttaccccgtt ttttacaaca
      661 aaagaccctg gaaaaggaac gggtcttggt ttgaatattg ttcacaagat tatcacgaat
      721 cacaggggca gcatcagcgc ggagagcgaa gaagaaaaag gagccacctt tgtagtgcgg
      781 tttcctatag cacaagaatc tcctgaagga gaagataagc aagtaaagac taatatgtat
      841 gatcaagagg agggaatctg atgtctacga ttttagtcat tgatgatgaa gaagacatcc
      901 gatctattgt taaacatcac ttggtcaaag cgggttataa ggtggtggag gccgaggatg
      961 gagagcaggc gatccaaaag attaatgaag gagataaccc gctgacaata gatgccgcga
     1021 tctgtgatat ccgcatgccc aagataaatg gggttgaggc cataagctat ttccggaagg
     1081 aatacccttc cgtgccggtg atcgtcatca ccgggttccc ggataccaac ctggcggtga
     1141 gcctcctgaa gaaaggggtc aaagattaca ttgtgaaacc ggtggagaaa gagaaattgc
     1201 ttgcagcggt aaaaaaagct gtttcagaaa aagctgaagc agatggaggc ttaatggcat
     1261 aacggttcct cttcgagaac cggcccgctc ttgccgtttc ctaaaacgat tagtacagcg
     1321 ggggctgggg gcctggcctc atgccagcga gatcgggatc cgtgccgtca tccgggggcc
     1381 gttcagcctc tttggctttt ttacgcgctt cacggcgcat cgtcttttca ttgcgtttct
     1441 caatctttgc gcgctcacgt aatcgctttg tcgctgtagc tttcggatga gctgacatat
     1501 ctgttcctcc tgacggtaac ggggtctcca gtgttcgtgc actctatcac actccctgac
     1561 aaaacgcaaa catatgcttc cttctctagt atcttccaga tttctgtttg acaatctctt
     1621 gaatttactt taaaatttga atattacttc aaatttaatt caaagataac atgaaagaat
     1681 ttcatcggat caaacagttg ccgccttatg tcttgtccgt ggtgaccgct gcgaagctgg
     1741 aggcgcggcg gcgcggggaa gacatcattg atttcgggat ggggaacccg gacgagccaa
     1801 cgccgcccca tatcgttgaa aaactgatcg aagcggcgaa aaaagctcag aatcaccgtt
     1861 actcggcctc acgggggatc accaaattgc gcctggcgat cgcagactgg tacaagcgca
     1921 actacgatgt gaagcttgat ccggagaccg aggcgattgt gacgatcggc tcgaaagaag
     1981 ggatttctca tcttgccctg gcagtgctgg gaccgggcga ttcggtgctg gtgccgaacc
     2041 cgacctatcc gatccatacc tacagtgtca tcattgcggg gggacaggtg ctggatgtgc
     2101 cgctacggcc cgagacgaat ttctttaatg aactggtgaa ggcctatgag caggccaagc
     2161 aaaagccgac gatgatgatc ttgaactttc cccacaatcc cacaacggcg atcgtggata
     2221 tcgggttctt taaaaagatc gtggaatttg caaaggcgca tgacctgatg gtcgtccatg
     2281 accttgccta tgccgatctc gcgtttgacg gctacaaggc acccagtttc ttgcaggttc
     2341 cgggagcaaa agaggtgggt gttgagttct tcacgctgtc aaagagctac aacatgcctg
     2401 gatggcgggt gggcttctgt gtcgggaacc gggagatcat cggggcgctc acgaagctca
     2461 agagctatct cgattacggg atgttccagc cgatccagat cgcaagcatc atcgcgctaa
     2521 acgggccgca ggactgtgtc cgagaaacgg tggaaaccta tcagcgacgg cgggatgtcc
     2581 tcgtggacgg tctcaaccgg attgaatgga aagtggacaa acctttggcc acgatgtttg
     2641 tctgggcccg catcccggag gagttccggg cgatgggctc gcttgaattc tcgaaattgc
     2701 ttttatccaa ggcgaaggtt gcggtttcgc ccgggatcgg attcggaaag tacggcgatg
     2761 actatgtccg gtttgcgctg gttgagaatg agcaccggac ccggcaggcg attcagggaa
     2821 tcaagaagat tttaaaagga aagagatgaa ggcgcggcat cagcgatcgg taatcaaggt
     2881 tggcctgatc ggccttggga cgataggcac cggtgtggcg aagctcatcc agtccaacca
     2941 ggacctcatc cgccgcaggc tcggctgcgg gctggaactg tccaaggtcg cggataagga
     3001 catcaagcgg gatcggggcg tctctttgcc gaagggggtg ctgaccacga aggtccagga
     3061 tctcatcgat gatccggaga tcgagatcgt ggttgagctg atggggggat atgatcccgc
     3121 caagaaattt atactgagcg cccttgccca tcgcaagcat gtcgtgacag cgaacaaggc
     3181 cctgcttgcg gttcatggag agcagatctt cgaggcggcc gagcaggcgg gctgcgacct
     3241 ggggttcgag gggagcgtcg gcggcgggat cccgatcatc cgtgccgtga aggaaggatt
     3301 ggccgcgaac aagatactct cgatttatgg gatcattaac ggcacctcaa attatattct
     3361 cacccagatg acagagaaca agcggtcctt caaagaggtt ttgaccgagg cccaaaaaaa
     3421 gggctatgcc gaggcggatc cgagcttcga cgtcgagggc atcgactcgg ctcataaact
     3481 cgcgatactg gtgactttgg ccttcggcac gccggtgacc tttaaagaga tctataccga
     3541 gggaatcaca cagatcacgc cactggatat cgagtacgcc cgggagctgg gttaccagat
     3601 caagctcctg gcaatcgcca agttgaacca gaaagagaaa gatcatactg ccattgaggc
     3661 ccgcgttcat ccaacgatga tcccggagaa ttatcttatc g
//
LOCUS       DATMKH010000575         1839 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1862237, whole genome shotgun sequence.
ACCESSION   DATMKH010000575 DATMKH010000000
VERSION     DATMKH010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1862237"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1839
                     /gene="secA"
                     /locus_tag="VNV63_06860"
     CDS             <1..>1839
                     /gene="secA"
                     /locus_tag="VNV63_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HXC62379.1"
                     /translation="TKTVALTEEGNPKVERLLGVDNLYDLSNMDLVHHVIQALKAHAL
                     FKRDVDYVVNDGEVLIVDEFTGRLMPGRRWSDGLHQAIEAKEGVKIANENQTLASITF
                     QNYFRLYKKLAGMTGTADTEATEFAKIYNLEVMVIPPNKTMIRIDNADVIYRTEQEKT
                     NAIIEEIVDCHQRGQPALVGTISIEKSEQLSSLLKKRGIKHSVLNAKYHEKEAEIIAQ
                     AGRKGAVTIATNMAGRGTDILLGGNPDFLFKRVLTQQPDLPPEEQGRIFDQIKAECEA
                     EKKEVVSLGGLHIMGTERHEARRIDNQLRGRSGRQGDPGSSRFYLSLEDDLMRIFGSE
                     RISGLMQKLGMEDGVPIEHRMVTKAIEGAQKKVEAHNFDIRKQLIEYDDVMNKQREVI
                     YEQRRQVLRGENIREEIMGIVEENVDGQLQLYCSKDLYPEQWDIKSLKDAFNRQFALH
                     LNRADSELREMGIEALREDLLRETEQAYQKKEQEFGSTLLRHLEKMVMLQVIDTHWKD
                     HLLSMDLLKEGIGLRGYGQKDPLIEYKREGFDMFSAMIEKIQTDAVERLFHVKAVREE
                     PVPGQAMARRQDIQMNRGELVSAAPAHRENKKVGRNEPCLCGSGKK"
BASE COUNT          477 a          464 c          560 g          338 t
ORIGIN      
        1 agaccaaaac tgtggccctg acagaggaag gaaaccctaa ggtggagcga ctcctcgggg
       61 tggataacct gtatgacctg tccaacatgg acctcgtcca tcatgtgatc caggcattaa
      121 aagcccatgc cctcttcaaa cgcgatgtgg attatgtggt caacgacggc gaggtgctca
      181 tcgtggatga attcaccgga cgcttgatgc cgggacgccg atggagcgac ggtctccatc
      241 aggcaatcga ggctaaagaa ggcgttaaga tcgcaaacga gaaccagacg ctggcctcaa
      301 tcacgtttca gaactatttc cggctctaca agaaactcgc cggcatgacc ggaaccgcag
      361 ataccgaggc gacggagttt gccaagatct acaacctgga agtgatggtc atcccgccca
      421 acaagaccat gatccgcatt gataacgcgg atgtcatcta ccgcaccgaa caggaaaaaa
      481 ccaatgcgat catcgaagaa atcgtagatt gccatcagcg gggccagccg gcgctggtag
      541 ggacgatttc gatcgaaaag tccgagcaat tgagcagcct gttaaagaag cgcggtatta
      601 aacattcggt cttgaatgca aagtaccacg agaaagaggc tgaaatcatc gcacaggccg
      661 ggcgcaaagg ggcggtgacc atcgcgacca atatggccgg ccggggaacg gacatcctgc
      721 tggggggaaa cccggacttt ctgtttaaga gagttctgac gcagcagccg gatcttccgc
      781 cggaggagca ggggcggatc tttgaccaaa tcaaggccga gtgcgaggct gaaaaaaaag
      841 aggtggtgtc attaggcggt cttcacatta tgggtaccga gcgccacgag gcacgccgga
      901 ttgacaatca gctccgcggg cgatccggac gtcaggggga cccgggttct tcccgctttt
      961 atctctctct tgaagacgac ctcatgcgga ttttcggctc ggagcggatc tccggcctca
     1021 tgcagaaact ggggatggag gacggggttc ccatcgagca tcggatggtg accaaggcga
     1081 tcgaaggcgc ccagaagaag gtcgaggccc acaacttcga tatccggaag cagctcatag
     1141 aatatgatga tgtgatgaat aaacagcggg aggtaattta cgagcaacgc cgccaggttc
     1201 ttcgtggaga aaatattcgt gaagagatca tggggatcgt cgaagagaac gtggacggcc
     1261 agctccaact ctactgtagc aaggacctgt atcccgagca gtgggacatc aaaagtttga
     1321 aggatgcctt caaccggcag tttgcccttc atctgaaccg ggccgattcg gagttgcggg
     1381 agatggggat cgaggccctt cgggaagatc tcctccggga aacggagcaa gcctaccaaa
     1441 agaaggaaca agagttcgga tctacgctgc tgcggcattt ggaaaagatg gtgatgctcc
     1501 aggtgattga tacccactgg aaagaccatc tcctgtccat ggacctcctg aaggagggga
     1561 tcggacttcg cggctatggt cagaaggacc cgctgatcga gtacaaacgc gagggtttcg
     1621 acatgttctc ggccatgatc gaaaagatcc agaccgatgc tgtcgagagg ttgttccacg
     1681 tcaaggcggt gcgcgaggag ccggtcccgg gacaggcgat ggcccgtcgg caggatatcc
     1741 agatgaaccg cggtgaattg gtcagtgccg ccccggccca tcgggagaac aagaaggttg
     1801 ggcgcaacga gccttgcctc tgcggcagcg gaaagaagt
//
LOCUS       DATMKH010000576         1046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2174670, whole genome shotgun sequence.
ACCESSION   DATMKH010000576 DATMKH010000000
VERSION     DATMKH010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2174670"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            184..810
                     /locus_tag="VNV63_06865"
     CDS             184..810
                     /locus_tag="VNV63_06865"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010494632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HXC62380.1"
                     /translation="MSEEPKWLGWARELQAIAQTGLHFSESEYDRERYRKILATSVEM
                     FAHSSLESPAFIRGLFENQTGYATPKVDVRGVVFRDNKLLLVQERSDGLWTLPGGWAD
                     VNDSPSEAVEREIVEESGFQAKAERLLAVLDRAKHGNEPPFPFHVYKLFILCRLAGGE
                     AKATMETTGVEFVGENEIPPLSLSRVTREQIQFCFDARKNPDGPCRFD"
BASE COUNT          219 a          296 c          284 g          247 t
ORIGIN      
        1 gagagcaatt tgcctgtaaa aaatcccaag agggagcttg acctgcacgg acgtacttcg
       61 ccctgaactc ctgaactcct gaactcctga gctcctgcaa ctcctgtctc ctgtctcctg
      121 tctcctgtct cctgtctccc accgctcgtt gacgttcatc ttccttcggc gacctttgtc
      181 ctcatgtcag aggaacccaa atggctgggc tgggctcgag aactgcaggc catagcacaa
      241 accggattgc acttctcaga atccgaatat gatcgcgagc ggtaccgaaa gattttagcg
      301 acctcggtgg agatgttcgc tcactcttcc ctcgaatcac cggccttcat tcgaggactt
      361 ttcgagaatc aaaccggata cgcgacgcca aaggtcgatg tgcgcggcgt ggtcttccga
      421 gacaataagc tattgttggt gcaggaacgg tcagatgggc tctggacctt gcccggaggc
      481 tgggccgacg taaacgattc cccatccgag gccgtggaaa gagaaatagt cgaagagtcg
      541 gggtttcagg cgaaggctga aagactgctt gccgtcctgg accgagccaa gcatggcaac
      601 gagccgccgt ttccgtttca cgtgtataaa ttatttatcc tctgccggtt ggcaggaggc
      661 gaagccaagg caaccatgga aacaacggga gtcgaattcg tcggcgaaaa cgagattccc
      721 cccttgtctc tctcaagggt cactcgcgaa cagatccaat tttgctttga cgcccggaaa
      781 aatcctgacg gcccttgccg atttgattag cctggaattt tctcggtttg tcagcgaatc
      841 gggttaggat tgggcatggc gatttgcatt tcaaaggggg acgaccagat ccgatgactc
      901 cggtggtttt gatagtcgcc ctggtctcga tcgggggttc cagtttgatt ctgcggatcg
      961 tgatcggata cctcgttcgc ccgcttggca cccgggtctc gctccgcaaa ccgtccatcg
     1021 atccgcgccg cattggccgc cggctc
//
LOCUS       DATMKH010000577         7700 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_912858,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000577 DATMKH010000000
VERSION     DATMKH010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 7700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7700
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_912858"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1366
                     /locus_tag="VNV63_06870"
     CDS             <1..1366
                     /locus_tag="VNV63_06870"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HXC62381.1"
                     /translation="ATVKGLSDVKGLLVNEAIEIKPLKLEKPGSPPEEEEATTIQGDA
                     ASIKSLSQTVRVDIGKLDSLLNVVGELVQTKAVLYQISKEWVTESGLTKKAVELQKVV
                     QSFDRQVKQLQEDLIEVRMIPISQVFDRLIRVARKLSKELGKEVDLQLSGEETRLDKS
                     MVEEIADPLMHMIMNAIDHGIESKEDRLKAGKPEAGVIQLKAAQKGNNVVIEVQDDGR
                     GIDLAKVYQTAIKKGLTDKNKEYEKREILEFLFMPGFSTRDQVTEISGRGVGLDVVAL
                     NISKLSGMVDVETELGRGTSFSITLPITLIIIKALIVRVGGETFAIPLSSVSESLMIE
                     QKDIRTVERREVTRLRDQTLSLLRLLDVFRLPRTEALDTRVYVIVVGLAEKRLGLVVD
                     AIEGQQEIVIKSIGGILRDAPGIAGAAELGNRKTILVLDVGTLIEETMRGKVSRRPSL
                     QPYS"
     gene            1699..1950
                     /locus_tag="VNV63_06875"
     CDS             1699..1950
                     /locus_tag="VNV63_06875"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HXC62382.1"
                     /translation="MGSSMRREDYRFGTKEICDLIGISARQLEYWVLIGVVTPKLERH
                     GAKVFKKFSQKDVELLQRVKDLTDEGVLVSRAADKVRQA"
     gene            1947..2462
                     /locus_tag="VNV63_06880"
     CDS             1947..2462
                     /locus_tag="VNV63_06880"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HXC62383.1"
                     /translation="MISPSSPQNSPDSSIEDAAAAGKIIQLLAFLLSDEEYGIDIRMV
                     KEIIRLTEITRVPRALSYIQGIISLRGTVLPIFDLRQLLSMPDSKLDKKSRILVVSLE
                     AGLIGIIVDAVTEVLELNEAELEPAPAVMTNLEADHIKGLGRYKGRLIVFLDLEKVYQ
                     ARHVQAKSVQG"
     gene            2514..4658
                     /locus_tag="VNV63_06885"
     CDS             2514..4658
                     /locus_tag="VNV63_06885"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HXC62384.1"
                     /translation="MSEKVKSNTPGYGLRFKFTAMFGALFVILSLILSWFSLSRTQAS
                     FEDELKSRGIALAKDLAYNSTYGMTIGDNKSLMGLVKGVVDKQDVVYAIILDAQGKVL
                     ADSDVAEVGKSYADETTRKGLAAKETIVLPVQTELWDEVYEVDNPVLIKTETGGVSGS
                     GLTENRIGLVRVGLSLKNLKARLESNLLFSIFLTSAVVGVGIVISSFFVRYIIKPIEQ
                     MAVIATQIANGDFSQTIQGQSQVQSNDEIGVLSTAFVAMSANLNQMIKKIRSAANNVA
                     SAADQISGTSRRASEGASVQAQATENTSSSMEEMNAAMKEIAESVDILSSAADASASS
                     ILEMSASINEVASNTANLSSSVEDTSASIIEMSASIKQVAQNSEILSSAAEQTTSAVN
                     EINASINEVEASSKEAAAASEKVTSDAQEFGVVAIEKTILGMNNIREAVEVSARVINK
                     LGERSEHIGKILTVIDEVTKQTNLFALNAAILAAQAGEQGKGFAVVAGEIKNLADRTA
                     SSTKEIAQLITSVQTEAKDAVEAIKTGSRSVEEGVRLAKDASDALTKILESTKHSTTM
                     ARDIERATTEQAKGIRQITEAMQQVNGMIRQIAKATQEQSRGSEQIMQAAERMRNVTR
                     QVRLSTDEQAKGSKQITQAVENVTDRVQQIAKAINEQKRGSQVIITSAEAIRGIAQQS
                     VQLVSQMNQAVETLTRQAALLQEEMTRFKVQG"
     gene            4663..5139
                     /locus_tag="VNV63_06890"
     CDS             4663..5139
                     /locus_tag="VNV63_06890"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HXC62385.1"
                     /translation="MPKVPETTKPKELRVVSFQVNEREYAVDIEKVVEIIYFKPVTPI
                     PRSPSFIEGIIDLRGSVIPIVDVKKRLGLASDPDLHPDYILIVKIQDRRLGIMVDRVR
                     EVLQIETARIEPHHKIVRGRHSEFLTGVCRVAERLILLLDIETLLSTEEKDRLEEI"
     gene            5148..7418
                     /locus_tag="VNV63_06895"
     CDS             5148..7418
                     /locus_tag="VNV63_06895"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HXC62386.1"
                     /translation="MKYITNHLEYKILVLITAVLILSFGTIFYLVSSHERNRAVSQDA
                     EKSALLAGSLHRTIDTNMMSFKADIVRHLIGDLKTLEGVVRVQIVRGKDDFRKTDSKG
                     TEEAFQDLKTIEEVNGRVPELAAAHPEWKTDHATKAVNVAQGVENPEFKRYFATFLKD
                     LSEATNDETRMKIAQKDYVYFEDLAGEPVLTYLKPLPNFQRCFLCHGGDHKMLGVLMI
                     STSMKSVNAETRKSQRYLLLISIGILLFLLLALKILMGRLVLIPVAEVVGHIQGIARG
                     EGDLTQRLATRSHDEIGQFAQWFNLFVEKLQSIVLNVSNTSKQVTHIAQEVVRGTKEI
                     LEGSQIQGKAVETTSEAITAMSLSIQKVAENTDVLSNLSEESSSAIIEMAAAIGEIAK
                     SSMALSDSVEATSTSIIQMSSSVKQISENVKVLASSADDTSSSMVRIDAATKQIRSNI
                     HETVELSKKVTEDAVKGGNSVKLTMGGITRIKESSQQVFTVIHSLRGRAQDIGKILSV
                     IDEVAEQTNLLALNAAIIAAQAGEHGKGFAVVAQEIKELAERTAASTQEIHQIINAVQ
                     IEARNAVETMNIGSKSVEEGVQLSKDAEMALSKILESSARSTERIREIANATDEQTSG
                     VVQVTEAMRKIDDMARQIAVTSEEQNKETEQIIQTTEYMRQIAKKVGIATQEQSQGNQ
                     QITEIVEKVNKKVKEIVKATSLQTGESREIVRAVERIKEVTIKNVKSVESVGKAVEEL
                     IRQATSLANEISKFHV"
BASE COUNT         2018 a         1794 c         2250 g         1638 t
ORIGIN      
        1 tgcaacggtg aaaggtctat ccgatgtaaa gggtctgcta gttaacgagg cgatcgaaat
       61 caaaccttta aaacttgaaa aacccggcag ccctcctgaa gaggaagaag cgactacaat
      121 acaaggagat gccgcttcga tcaaaagcct gagccagacg gttcgagtgg acatcgggaa
      181 gttggacagc cttctcaacg tggtggggga actggttcag acgaaggcgg tgctctatca
      241 gatcagtaag gaatgggtga ctgagtcggg tttgacgaag aaggcagtcg agttgcaaaa
      301 ggtggtccag agcttcgacc gccaggttaa acagcttcag gaagatttga ttgaggtgcg
      361 catgattccc atcagtcagg tttttgatcg cctgattcga gtggcgcgaa aactctcaaa
      421 agagcttgga aaagaggttg accttcagct ttcaggtgaa gaaacccggc tggacaagtc
      481 tatggttgag gagatcgcgg atcccttgat gcatatgatc atgaacgcaa ttgaccacgg
      541 catcgagtca aaggaggacc gtctcaaggc cggcaaaccc gaggcgggag tgattcagtt
      601 gaaggctgcg cagaagggaa acaatgtcgt gattgaggtt caggatgacg gccggggaat
      661 tgatctggcc aaggtatatc aaacagcgat caagaaaggg ctaaccgata agaacaagga
      721 atatgagaaa cgggagatcc tggaattttt gtttatgccg ggcttcagca ccagggatca
      781 agtaactgaa atctcaggcc ggggggtggg cctggatgta gttgccctaa acatctcaaa
      841 actcagcggg atggtagacg tggaaaccga gttgggacgg ggaaccagtt tttcaattac
      901 gttaccgatc acgctgatca tcatcaaggc cctcatcgtc agggtcggag gcgagacttt
      961 tgcgatcccg ttgagttcgg tctctgaaag tctgatgatt gagcagaagg atattcggac
     1021 tgtggaacgg cgagaggtaa cacggctccg agatcagact ctctcgctcc tgcgtctgct
     1081 cgatgtcttc cgattaccta ggacggaggc cttagacaca agggtgtacg tgatcgtggt
     1141 aggtttggcc gaaaaaaggt tggggcttgt ggtggatgcg atagaaggac aacaggagat
     1201 tgtgatcaaa tccatcgggg gaattcttcg cgacgctccg gggattgcag gggccgccga
     1261 gcttggaaat cggaagacga tcctcgtcct ggatgttgga acacttatcg aagagacgat
     1321 gcggggaaag gtgtcgcgcc gaccgtctct tcaaccatat agttgactca aacgcccatc
     1381 ggcatcgtat catggcgggt gcctgctaac tctttatccc ccctgccatc ggatcttctt
     1441 cagactttaa aatcctttag acccgccatt ttttgtccat agaaagaaac cggttacata
     1501 tttgaacggt cttttgggcg agcagggatg catcattgga caaggtcaaa tctctaacca
     1561 tctgtaaagt aaatcaagtc tattatccgg ggagaaaccg gtttagtggc ataggctttg
     1621 cattgatcct cgctgggatc tcacatccca cagggttgtg ggatcctgtt gaaaatgtct
     1681 gatatgaaag gtaagaagat gggatcatct atgcggcgtg aagattatcg gttcgggacg
     1741 aaggagattt gtgacctgat cggtatttcg gcccggcagc ttgagtactg ggttttgatc
     1801 ggggtggtga cccccaagct tgagcgccac ggggccaagg tctttaaaaa attcagtcag
     1861 aaggatgtgg aactcctgca acgggtgaaa gacttgacgg atgaaggggt gctggtgagt
     1921 cgggcagcgg acaaggtgcg tcaagcatga tcagcccttc ctcaccacaa aattctcccg
     1981 attcatcaat tgaggatgca gcagccgccg gtaaaattat tcaattgctg gcttttctcc
     2041 tcagcgatga agaatacgga atagacattc ggatggtcaa agaaattatt cgattaaccg
     2101 aaatcactcg ggtcccgcgg gccctgtcct acattcaggg gatcatctcg cttcgaggca
     2161 cggtcctgcc catctttgat ctccggcaac tgctttcgat gcctgattcc aaactggaca
     2221 aaaagagccg gatcttagtc gtgagtttgg aggcgggcct catagggatt attgtagatg
     2281 ccgtgaccga ggtcttggag ctcaacgagg cggagcttga accagcgccc gccgtgatga
     2341 caaacttgga ggcggatcac ataaaagggc tgggccgtta taaagggcgc ctgatcgttt
     2401 tcttagacct cgaaaaggta tatcaggcca gacacgttca agctaaaagc gtccaggggt
     2461 aagacagttc caagtgacat taaaaatctt aagcacgggg taggaaggct gtcatgtcag
     2521 aaaaggtaaa aagcaacaca ccgggatatg gcttaaggtt caagtttaca gccatgttcg
     2581 gtgctctctt tgtaatactg agcttgatcc tgagctggtt ttccttaagc cgcacacaag
     2641 cctcgttcga ggatgagctc aaaagtcgag gcattgcact ggctaaggat ctggcctaca
     2701 acagtacgta tggaatgacc atcggcgaca ataagtcgct catgggcttg gtaaaaggtg
     2761 tcgtggacaa acaggatgtg gtctatgcga tcatcttgga tgcacagggc aaggtcttgg
     2821 cagacagcga tgtcgcggag gttggcaaaa gttatgccga cgaaacgacc cgaaagggcc
     2881 tggcagccaa ggaaacaatc gttctccccg tacagaccga gctgtgggac gaggtctacg
     2941 aagtcgacaa cccggttctg ataaaaacag agacgggggg tgtttcgggc agtggattga
     3001 cggagaacag gatcggattg gtgagagtcg gtctgtcatt gaaaaacctc aaggcacggc
     3061 ttgagtcaaa cctccttttc agcattttcc tgacctcagc ggttgtcgga gttggaattg
     3121 tgatctcgtc cttctttgtg cgttatatta ttaagcccat cgagcagatg gcggtcatcg
     3181 caacacagat tgcaaacggg gatttcagtc agacgattca gggtcagtcc caagtccagt
     3241 caaatgatga gatcggggtt ctgagcacgg catttgtggc gatgtcggcc aatctcaacc
     3301 agatgatcaa aaagatccgg tccgcagcga acaatgttgc atccgctgcg gatcagatca
     3361 gcggtacatc gcgacgggcg agtgaagggg ccagtgtcca ggcccaagcg accgaaaaca
     3421 cgtcctcttc aatggaagag atgaacgctg ccatgaaaga gatcgccgag agcgtggata
     3481 ttttatcctc ggccgccgat gccagcgcct cttcgatctt ggaaatgagc gcctcgatca
     3541 atgaggtggc cagcaacacc gccaatctca gcagctctgt ggaagacacc tccgcctcga
     3601 tcattgagat gtcggcttcg atcaaacaag tggcgcagaa ttctgagatc ctttcctccg
     3661 ctgcggaaca gaccacctct gccgtaaacg agatcaacgc ctcgattaat gaggtggagg
     3721 ccagctccaa ggaggccgct gcggcatcgg agaaagtgac ctcggatgca caggagtttg
     3781 gagtggtggc gatcgagaaa acgattctgg ggatgaacaa catccgcgag gccgtagagg
     3841 tatcggcacg cgtgatcaac aaattggggg aacgatccga gcatatcggt aagatcctga
     3901 ccgtgattga cgaggtcacc aagcaaacca acctctttgc cctcaacgcc gcgatcctgg
     3961 ccgcccaggc aggggagcag ggcaagggtt ttgcggtcgt ggcgggcgag atcaagaatc
     4021 tcgcggaccg caccgcctcc tcgacgaagg agatcgccca actcatcacc agcgtccaga
     4081 cagaggctaa agatgcggtg gaggcgatca agaccggttc ccggagcgtg gaggaaggcg
     4141 tgcgtctggc aaaagacgca agcgatgcgc tgacgaagat cctggaaagc acaaagcact
     4201 ccaccaccat ggctcgggat atcgaacggg ccacgacgga gcaggccaag ggcatccgac
     4261 agattaccga ggcaatgcag caggtcaatg gaatgatccg acagatcgcc aaggcgaccc
     4321 aggagcagag tcgcgggagc gaacagatca tgcaggcggc tgagcggatg aggaacgtca
     4381 cgcgccaggt gcgcttgtcc acggatgaac aagcgaaggg gagcaagcag atcacccagg
     4441 cggtggagaa cgtcacggat cgagttcaac agatcgccaa ggccatcaat gagcagaagc
     4501 gagggagtca ggtgatcata acgtcggcgg aagcgatccg aggcatcgca cagcagagcg
     4561 ttcagttggt cagtcagatg aatcaggcgg ttgaaacatt gacccggcag gccgcgcttc
     4621 ttcaggagga gatgacccgc ttcaaggtgc agggatagca ccatgcccaa agtgcctgag
     4681 actaccaagc ccaaggaact ccgtgtggtg agctttcagg tgaatgagcg tgagtatgct
     4741 gtggatatcg aaaaagtggt ggagatcatc tattttaaac ccgtgacgcc cattcccagg
     4801 tctccctctt ttatcgaagg catcattgac ctgcgcggct ctgtgatccc gatcgtggat
     4861 gttaaaaagc ggctggggtt agcttccgac cccgacctcc atcccgatta catcttgatc
     4921 gtcaagatcc aggatcggcg cttgggcatc atggtggatc gtgtccgtga ggttctgcaa
     4981 atcgagacgg ccaggatcga gccacaccac aagatcgtac ggggaagaca ttcggaattt
     5041 ctgaccggcg tctgcagggt tgcggaacga ctcattctgc tgctggatat cgaaacctta
     5101 ttgagcacgg aagagaagga ccgtttggaa gagatttagg cgctgagatg aaatacatta
     5161 cgaatcatct ggaatataaa attcttgttt taattactgc tgtcctgata ctttcttttg
     5221 gcacgatctt ctaccttgtc tcttcgcacg aacggaaccg tgcggtgagc caagatgccg
     5281 agaagtccgc cctgctggca gggtcccttc atcggactat tgataccaac atgatgagtt
     5341 ttaaggcaga catcgtccgc catctcatcg gagacctcaa gacactggag ggtgtggtcc
     5401 gggtccagat tgtcaggggc aaagacgatt tcagaaagac agacagcaaa ggaacagaag
     5461 aggcctttca ggatctcaaa accattgaag aggtcaatgg ccgagtgcct gagctagcgg
     5521 cggcccatcc ggaatggaag acggatcacg cgacaaaagc ggtgaatgtg gcgcagggtg
     5581 tggagaaccc tgaatttaag cgttacttcg caacattttt aaaagacctc tccgaagcga
     5641 ccaacgatga aaccaggatg aagatcgctc aaaaggatta cgtatatttt gaagacctgg
     5701 ctggagagcc ggtactgacc tacctgaaac cgctgccaaa tttccagcgc tgttttctct
     5761 gccatggagg agatcataaa atgctcggcg tcctgatgat ttccacctcg atgaagtcgg
     5821 tcaatgcgga gacccgaaaa agccaaaggt atcttcttct aatttccatc ggaattctgc
     5881 tgtttcttct gttggcgttg aagatcctga tggggcggct ggttttgatc cccgttgcag
     5941 aagtcgtcgg acacattcag ggcattgccc gcggtgaagg ggatctgact caaaggcttg
     6001 cgacacgatc tcatgatgag attggtcagt ttgctcagtg gttcaatctc ttcgttgaaa
     6061 aacttcagag cattgttttg aatgtgtcca atacctccaa gcaggtcaca catatcgctc
     6121 aagaggtggt tcgggggact aaagagatct tagaaggttc tcagattcag ggcaaagccg
     6181 tggagactac ttccgaagcg atcacggcta tgagtctctc aatccagaaa gttgcagaga
     6241 ataccgatgt gttgtctaac ctctccgaag aaagcagctc cgccattatc gagatggcgg
     6301 ctgcaattgg tgaaatcgcc aagagcagca tggcgctttc cgactctgtt gaggcgacca
     6361 gcacctcgat cattcagatg tcttcctctg tgaagcagat cagtgagaat gttaaggtgc
     6421 tggcctcttc cgcggatgac acttccagct cgatggtgcg gatagatgcg gcgaccaagc
     6481 agatccggtc gaatatccat gaaaccgtgg agttgtccaa gaaagtcacg gaggatgcag
     6541 tcaagggagg caactcggtg aagcttacga tgggcgggat cacacgcatc aaggaatcat
     6601 cccagcaggt gttcacggtg atccacagtc ttcgcgggcg agcacaggat attgggaaga
     6661 tactgagtgt gattgatgag gtggctgagc agacgaacct cttggcgctg aacgcggcga
     6721 tcatcgcggc acaggcgggc gaacacggca agggttttgc tgttgttgct caggagatca
     6781 aagagttggc ggaacggacc gctgcttcca cccaagagat tcatcagatc atcaatgcgg
     6841 ttcaaatcga ggcacggaat gcggtcgaga cgatgaatat cggctccaag agcgtggaag
     6901 aaggagtaca gctctccaag gatgctgaaa tggcgttgag caagattctg gaaagttcgg
     6961 cccgctcaac cgaacggatt cgagagatcg ccaatgcaac ggatgagcag actagcgggg
     7021 tggtacaggt caccgaggcg atgagaaaga tcgatgatat ggcccgccag atcgccgtga
     7081 ccagcgagga gcagaacaaa gagaccgagc agatcatcca gaccacagag tacatgaggc
     7141 agattgccaa gaaggtgggc atcgcaacgc aggagcagtc gcaagggaac caacagatta
     7201 ccgagatcgt agagaaggtt aataagaagg tcaaggagat tgtgaaagcg accagtcttc
     7261 agaccggtga gagtcgtgag attgtccgag cggttgaacg gatcaaggag gttacgatca
     7321 agaatgtgaa atccgtggag tccgtcggaa aggcggtgga agagttgatc cgccaggcta
     7381 cctcgctggc caatgagatt tcgaaattcc acgtttaatt tagaatagga gtgtaatcta
     7441 tgccgctgac ttgtcctgaa tgtttacgat cctttgattc ccgtgaaccg ttgcagcccg
     7501 gagaaataaa aatctcctgt ccccattgtc aggcctcgat gattgtggtg accacggtaa
     7561 aggccgcagg gacaccgcga gctcggcccc gccaaatcct ttctccggag cggctcgttc
     7621 tggtggcggt ggaaggaaat gccaccaagg agatgataca ggaagtgctt caggaagggg
     7681 gttatgaggc gatcatggcc
//
LOCUS       DATMKH010000578         1550 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_590110,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000578 DATMKH010000000
VERSION     DATMKH010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590110"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VNV63_06900"
     CDS             complement(<1..195)
                     /locus_tag="VNV63_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62387.1"
                     /translation="MDLGLTAELDMRVEISELGVIDNLGTTSRLSAASTGLGLSIFDP
                     MRSPVEQLDSLPPSGCLFSAG"
BASE COUNT          347 a          400 c          415 g          388 t
ORIGIN      
        1 tcctgcggag aacaggcacc ccgaaggcgg aaggctatcc agttgttcaa cgggagacct
       61 catcgggtcg aaaatggaca aaccaagccc ggtcgacgcc gcggaaagtc gagaggtggt
      121 gccgagatta tcaatgactc caagttcgga gatttcaact cgcatgtcga gttcagctgt
      181 cagccccaag tccaacagtg gtctctaatt gcgcggcggc tatgaggtca agattgaaac
      241 cgactctggc tggtcatcat gcgggcggag tggatggcta cttggtcccg tcgctcgaaa
      301 tgcccgcaag ccaggagagt ggcagaattt cgatattcca tttggttgga cgaatgatca
      361 ctgtggtcca gaatggtcag acgatcattg acgaccagga aatcccggct atcacgggga
      421 ggcgctcttg acggcgaatg gctgggtccg atttacctgc agggcagtga ataaggacac
      481 gcagcttatc ggaatatggt catcacgcca acgagataat cagggaaggg aatccagttt
      541 ctgagagatg tcccctaatc cacactggcg gagaggggat catcctaacc caccactcgt
      601 aagctagtct cggttgcccc caacgcgact ctgaaaaatc ttcgctggac aaacgaattg
      661 tgcgcaggta agtccatcat ccgatggtcc taattgtttc cggcatggcg aaatcgcttg
      721 ctgagaaacg taggcgagtc gactcttttg gggaagttcg gttatgcggt cgcaaaggca
      781 cattcgagca acccagcagc atcccggaag tccacgaatc cctggtcgtg accttcgggt
      841 cacgattact tagtccttgt tctttgcttc tttgcttctt cgcttcttct ctttggcgct
      901 tcttcctttt cttctttctc agaggcgttt gtggacgcct ttcttgaaat tctcgagcat
      961 gcggtcacag gtccgcatcg tcgccgaaag ctacggcaga agggggaagc ccatgcgggg
     1021 atgcctgatt tcaacagtgg tcccgcacag cagtcaccgg ttaactgaat aggctacctg
     1081 cctccccgca gggaggggag tgtgattttc aggttcgtct tctttgaaga agtgtaagtg
     1141 agtggacacc ttggtggttc aactaaggga aagggacgag tggcggagtg ttcccccgag
     1201 gtttgtgatg gtcagtctaa catcggaaaa acatagaaag cctttggtct ggattcttga
     1261 cggaaacagg ctatcgtttc tggtcttgta tgcagatcgc agcctacaat tttcatctcg
     1321 gcctccttcg tcccgagcct ttttggttgg acgccgcaaa gtctactcgg tccgttgtga
     1381 ggccaacgtc gctatgacat cagacacgtg atatttcgcc aaccatgctc ttcagatctt
     1441 gagatgccgc catccccatc gatttcatct ccatcgattt attgactttc ccattctgcc
     1501 aacgtaatat ttgccacgct tgattggctc tgattggccc aaaccatctc
//
LOCUS       DATMKH010000579         1943 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3461052, whole genome shotgun sequence.
ACCESSION   DATMKH010000579 DATMKH010000000
VERSION     DATMKH010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3461052"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..1129)
                     /locus_tag="VNV63_06905"
     CDS             complement(134..1129)
                     /locus_tag="VNV63_06905"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylcobamide--CoM methyltransferase"
                     /protein_id="HXC62388.1"
                     /translation="METFLASAGSYPRIGAEPKHQRLRKAYGCWERGEITKEGFEKIQ
                     QSMVKAAIHEQDKAGLDLLTDGQIRWHDPVSHLAGKLEGVKIDGLLRFFDTNFYFRQP
                     VITARLQWKGPLVQDEFTFAKKITKKPVKAILTGPYTLAKLSIIQTDAYPHLQDLVLD
                     FAELMSREIEGLARAGATMIQIDEPAIIRHPNDFAILRQAVTVLARKKGNSRLALYTY
                     FGDAAPIYDQLQSLPVEILGLDFTYSPQLADRVERDGSDKQLGLGLIDGRNTRLETKE
                     EIFKLLNKILPRLKGDICYINPSCGLEYLPRDKAYRKLANMVKLKKMFQERGVRP"
     gene            complement(1248..>1943)
                     /locus_tag="VNV63_06910"
     CDS             complement(1248..>1943)
                     /locus_tag="VNV63_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HXC62389.1"
                     /translation="GTGQSTMDGILRATNRLIAGTVFVVNGYGWCGRGIAMRAKGMAA
                     DVVVTEVDPLKAIEAVMDGYRVMPMEEAAKIGDFFVTVTGNIHVIRREHFAVMKDGAI
                     VCNSGHFNVEIDIPALTKMSRRHRTIRDFVEEFTLSDGRRINLLAEGRLINLSAAEGH
                     PPDVMDMSFANQALSAEYMAKEGKKLTKKVYPVPLSIDKEIARLKLESMGSRIDRLTK
                     QQEKYLASWEMGT"
BASE COUNT          429 a          534 c          485 g          495 t
ORIGIN      
        1 ttgggcttcg caaggtttcc ttttgccgcg cggactcgtg cctttaacaa atagtcgggt
       61 tttgggaaac ttccaacgct ggtggtagga aacatgggaa ggtttagtcc aagggtcttt
      121 aacttttttc gactcatggc cgtacacctc tctcttgaaa cattttttta agtttcacca
      181 tgttcgccaa ttttcgataa gccttatcgc gcggaaggta ctccaaaccg caggacggat
      241 tgatgtagca gatatcccct tttagccttg gaagaatctt gttgagcagt ttgaatatct
      301 cctcctttgt ctcaagacga gtgttccggc cgtcaatgag accgagaccc aactgcttat
      361 cggatccatc ccgctctacg cgatccgcaa gctgtggact gtaggtaaaa tccaggccca
      421 ggatctcaac cggcaggctc tggagctgat cgtagatcgg cgcggcatcg ccaaaatagg
      481 tatagagggc cagcctgctg ttgcccttct tccgagccag aacggtgacg gcttgccgga
      541 ggattgcaaa gtcgttcgga tggcgaatga tagcgggctc gtctatttga atcatcgtgg
      601 cccctgcccg tgcaagacct tcgatctcac gcgacataag ttccgcaaaa tccaggacca
      661 ggtcttgaag atgagggtat gcatccgtct ggatgattga gagtttagcc aaagtgtaag
      721 gtccggtcaa aatcgctttc accggttttt ttgtgatttt ctttgcaaaa gtgaactcgt
      781 cctggaccaa aggccctttc cattgaagtc ttgccgtgat gacgggctga cgaaaataaa
      841 aattcgtatc aaaaaaccgt aaaaggccgt cgatcttaac cccctccagc ttcccggcca
      901 ggtgagaaac gggatcatgc cagcggatct gtccatcggt gagcagatcg aggcccgctt
      961 tgtcctgctc atggatggcg gccttgacca tggattgctg aattttttca aaaccctcct
     1021 tagtgatctc gccccgttcc caacagccgt aggccttgcg taaccgctga tgtttaggct
     1081 cggcgccaat gcgcggataa ctcccggcac tggctaggaa tgtttccatg attatcctcc
     1141 gttcggtctc gggaagtctg tggataggaa aagagccatt ataccaccac agctaataat
     1201 agattgtcaa ggataaaata gctcttcagg tttgaacgag cgaggagtta cgtccccatc
     1261 tcccacgagg caagatactt ctcctgctgc ttggtcaatc ggtcaatgcg gcttcccatg
     1321 ctttcgagtt ttaggcgggc gatctccttg tcgatggaga gcggtacggg atacaccttc
     1381 ttcgtcagtt tctttccttc cttggccatg tactcggctg aaagcgcctg gttggcaaaa
     1441 ctcatgtcca tgacatcggg gggatgtcct tccgctgccg aaagattgat gaggcgccct
     1501 tctgccaaaa gattgattcg tcgtccatcg gaaagtgtga actcttctac aaagtcacga
     1561 atggtccggt gacgacggct catcttggtc agagccggga tgtcgatctc tacgttgaaa
     1621 tggcctgagt tgcagacaat tgccccgtcc ttcatcaccg cgaaatgctc tcttcggatg
     1681 acatgaatat ttccggtaac cgtcacaaaa aaatcgccga ttttggctgc ctcttccatc
     1741 ggcatcacgc gatagccgtc catcaccgcc tcaatcgcct tcagggggtc cacttcggtg
     1801 accaccacat ccgccgccat gcccttcgcc cgcatcgcga tcccgcgtcc gcaccagccg
     1861 tacccgttca cgacaaagac cgtcccggca atcagccgat tggtcgccct gaggatgccg
     1921 tccatcgtac tctgaccggt tcc
//
LOCUS       DATMKH010000580         2700 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1554883, whole genome shotgun sequence.
ACCESSION   DATMKH010000580 DATMKH010000000
VERSION     DATMKH010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1554883"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..580
                     /locus_tag="VNV63_06915"
     CDS             44..580
                     /locus_tag="VNV63_06915"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HXC62390.1"
                     /translation="MHTIRFKRLLGFIMSIFLLSAVFAHAESLKIGTIDAQKVLENTK
                     AGKKAKATMEEFVKSRQKIIDLEEQDIKNLEDELAKQGPVLSADAKKDKQETFQRKML
                     DYQKKVSELNKEVQNKKKEVLEDFNKTLEGVVKSIAEKEGYTVILDRNSEGGVILYAK
                     ESVDLTDKVVQEFNKSAQ"
     gene            596..1657
                     /gene="lpxD"
                     /locus_tag="VNV63_06920"
     CDS             596..1657
                     /gene="lpxD"
                     /locus_tag="VNV63_06920"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HXC62391.1"
                     /translation="MKTARLKEIADLVQGKIVGDPTITITGVNSIREAQRGEITFLAN
                     PRYRADLEKSTASAVVVAKSLDGFSIPMIVVENPYYAFAQIVKFFYTLSYHPLGVSEQ
                     AIVGAGVVMGKDVSIHPLAVIGAGAHIGDRVTIYPGCYIGAETEIGDESLIYPNVTIR
                     ERIKIGKRVIIHSGTVIGSDGFGFSTHQGRHEKILQIGTVVVEDDVEIGSNVSVDRAA
                     LGKTVIKRGTKIDNLVQIAHNVVIGEDCLIVAQVGISGSTEIGHHVILAGQVGLVGHI
                     KLGDHVIVGAKSGVSNDLASRSVVSGIPVIEHKTWLKTQAILPHLPEFRKRIIALEDR
                     IKQLETLLQQDKHEGKEDD"
     gene            1650..2114
                     /gene="fabZ"
                     /locus_tag="VNV63_06925"
     CDS             1650..2114
                     /gene="fabZ"
                     /locus_tag="VNV63_06925"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HXC62392.1"
                     /translation="MIDTKEIQKILPHRYPFLLVDRILDIVEGKKITGSKNVSINEPF
                     FQGHFPGHPIMPGVLIIEAMAQVGIVLAHKSPDASDSPEGKKGGQRLVYFTGIDKAKF
                     RKPVFPGDQLRLELEVLHVRSPFWKLRGMAYVGDQLVCEAELTAMFVKEEKR"
     gene            2116..>2700
                     /gene="lpxA"
                     /locus_tag="VNV63_06930"
     CDS             2116..>2700
                     /gene="lpxA"
                     /locus_tag="VNV63_06930"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009371901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HXC62393.1"
                     /translation="MTSSIHATAIVHPDAKLAEDVVVGPYCLIGEHVKIGRRVKLMSH
                     VAIEGWTDIGEDCQFFPFSSIGTIPQDLKFQGEMSRLTIGKKNVFREFVTLNRGTTEG
                     GGVTKIGDHNFLMAYVHVAHDCQIGDHIVMANAATLAGHITIQDYAIVGGLVGIHQHV
                     RIGCHAMIGGCSAVGMDVPPFTTAAGYRAKLYGLN"
BASE COUNT          715 a          585 c          761 g          639 t
ORIGIN      
        1 cacgttgttt tagtttaaca ttaatttaat aggagagagt tgaatgcata cgattcgatt
       61 taaaagactg ctcggtttta tcatgagcat tttcctttta tccgcagttt ttgcccatgc
      121 tgagagcctt aagatcggca ccatagatgc ccaaaaggtt ttagagaata ccaaggccgg
      181 gaaaaaggcc aaggcgacga tggaggagtt tgtaaagagc agacagaaaa ttattgatct
      241 ggaagagcag gacatcaaaa acttggaaga cgagctggcg aaacaggggc ctgtgttaag
      301 tgctgatgcg aaaaaagaca aacaagaaac atttcaacgg aagatgctcg actaccaaaa
      361 gaaggtgtcc gagctcaaca aagaggttca aaacaagaag aaagaggtat tagaggattt
      421 caacaagacg ttggaagggg ttgtcaaaag tatagcagaa aaagaaggtt atacggttat
      481 cctggatcga aattcagaag gtggagtcat tctctatgca aaagaatcgg ttgatcttac
      541 agacaaagtg gtccaggaat ttaataagag tgctcagtga gggtggacta aatcggtgaa
      601 aacggcccgg cttaaagaga tcgctgacct ggttcaaggg aagatcgtag gggatcccac
      661 gattacgatc acgggggtga acagcatccg tgaggcgcaa agaggtgaga tcaccttctt
      721 ggccaatccg cgatacagag cggatctcga aaaaagcact gcttccgcgg ttgtggttgc
      781 aaagagctta gatggtttct cgatcccgat gatcgtggtc gagaaccctt attatgcttt
      841 tgcacagatc gtcaaatttt tctatacgct ctcctatcat ccgctcggtg tgagcgagca
      901 agcgattgtc ggggctgggg tggtgatggg caaggacgtt tcgatccatc cactggcagt
      961 gatcggggct ggtgcacata tcggtgatcg ggtgacgatt tatccggggt gctatatcgg
     1021 tgcagagacc gagatcgggg atgagtcgtt gatctatccg aatgtaacga ttcgtgaacg
     1081 gattaaaatc gggaaacgcg tcatcattca tagcgggacc gtgatcggga gcgacggttt
     1141 tggtttttcg acccatcaag gccgtcatga aaagatcctt cagatcggga ccgtggtcgt
     1201 cgaagatgat gtcgagatcg gctccaatgt ctctgtggat cgggcggctc ttggcaagac
     1261 cgtgatcaaa cgcggtacaa agattgataa cctggttcag atcgcccata atgtagttat
     1321 cggggaagac tgtctgattg tggcccaggt cgggatttcc ggcagcaccg agatcggcca
     1381 tcatgtcatt ttggccggtc aggtgggtct tgtcggtcac atcaagctcg gagaccatgt
     1441 gatcgtgggc gcaaaatcag gggtgagcaa cgatcttgca tcgcgcagcg tggtttctgg
     1501 aatcccggta atagaacata aaacctggct taagacccaa gccattcttc ctcatttgcc
     1561 tgagttcaga aaacgcatca tcgcattaga agacagaatc aaacagttgg agaccctgct
     1621 ccagcaagac aagcatgagg gaaaagaaga tgattgatac aaaagagata caaaaaatct
     1681 tgccccatcg ttacccgttt cttctcgtgg atcggatttt agatattgtg gaagggaaaa
     1741 aaatcactgg ttctaagaat gtcagcatca atgaaccttt tttccaagga cattttcccg
     1801 gacatcccat catgccgggt gtcttgatca ttgaggcgat ggcccaggtc ggcatcgtcc
     1861 ttgcccacaa atcgccggat gcgtccgatt ctccggaagg caaaaagggc gggcaacggc
     1921 tggtctactt caccgggatt gacaaggcca agttccgaaa accggttttt ccgggggacc
     1981 aacttcggtt agaactggag gtgcttcatg tgcggtcccc cttctggaag cttcgcggga
     2041 tggcctatgt cggggatcaa ctggtctgtg aagcggaatt gaccgcgatg tttgtaaaag
     2101 aggagaaacg atgagatgac gagctcgatt catgcaaccg cgattgttca cccagatgcc
     2161 aagctggcag aggacgtggt cgtcggccct tactgtctga tcggcgaaca tgtcaagatc
     2221 ggtcgccggg tcaagctgat gtctcacgtc gcgatcgagg gttggacaga catcggagag
     2281 gactgtcagt ttttcccgtt ttcttcgatt ggaacgatcc cccaggattt aaaatttcag
     2341 ggggagatgt cccggcttac catcggaaag aaaaatgtgt ttcgggaatt cgtaacgctc
     2401 aatcgcggta cgactgaggg aggcggggtg acgaagatcg gggaccataa ttttttgatg
     2461 gcctatgtcc atgtcgccca cgactgccag atcggagatc atatcgtgat ggccaacgcg
     2521 gccactctgg caggccatat cacgatccag gactatgcca tcgtgggcgg gctggttggg
     2581 attcatcaac atgtccgaat cggctgccat gccatgatcg gcggttgctc cgcagtcgga
     2641 atggatgtcc cgccgtttac aactgctgcc ggatatcgtg caaagcttta tggtttaaac
//
LOCUS       DATMKH010000581         1114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1232389, whole genome shotgun sequence.
ACCESSION   DATMKH010000581 DATMKH010000000
VERSION     DATMKH010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1232389"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="VNV63_06935"
     CDS             complement(<1..408)
                     /locus_tag="VNV63_06935"
                     /inference="COORDINATES: protein motif:HMM:NF014500.4"
                     /note="member of the Gnt family of gluconate transporters;
                     not involved in gluconate transport; unknown function;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease DsdX"
                     /protein_id="HXC62394.1"
                     /translation="MASDPVRLVVVVIAILALIVLITKFRIHPFIALILASWFLGIAC
                     GLPLVEVIKHFEKGFGDVLSFVGIVIGLGAMLGGILISSGGADVLANDLIALGGKKWI
                     PWTMFLASLLIGLPLFFEVGFVLLVPLAFVISKR"
     gene            complement(515..>1114)
                     /locus_tag="VNV63_06940"
     CDS             complement(515..>1114)
                     /locus_tag="VNV63_06940"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HXC62395.1"
                     /translation="GYPKGQTIVRGFEDPIKSSSHLVVLYGNLAPEGAIAKISGKEGM
                     RFKGKARVFESEEKALRAILDDKIRKGDVIVIRYEGPKGGPGMREMLAPTSAIMGKGL
                     GKDVALMTDGRFSGGSHGFVIGHVTPEAFVGGPIAIIQNGDRIEIDGEKKVVDLKISK
                     AELAARLKKWKQPKPRYVKGVLAKYAKQVSSASLGAVTD"
BASE COUNT          272 a          319 c          275 g          248 t
ORIGIN      
        1 tcgttttgag atgacgaaag cgagcggcac caggagaacg aaacccacct caaaaaacaa
       61 aggaagcccg atgagcaggg aggccaaaaa catcgtccag ggaatccact ttttcccgcc
      121 cagagcgatc agatcatttg ccaggacatc cgctcctcct gaagagatca gaattcctcc
      181 taacatcgcg ccaagtccta tgacaatgcc gacaaagcta aggacatcac caaatccttt
      241 ttcaaagtgt ttaatcactt ccacgagtgg gagcccgcaa gcaatcccga gaaaccagga
      301 cgcaagaatc aacgcgatga atgggtgaat acggaacttc gtaatgagaa cgatcaatgc
      361 aagaattgca atgacgacga ccaccaggcg gacaggatca gaggccatga gagatcaggg
      421 gttagattgg ttgcgaggcg tgcgcagggg gcgatgaagc atcaattgcg aattttaaac
      481 cacagatttc acagcatcca ttgcggatgc tcacctaatc ggtgaccgcg ccaagcgacg
      541 ccgaactgac ttgcttggcg tactttgcca ggacaccctt tacatatctc ggcttcggct
      601 gtttccattt tttgaggcgc gcggctagtt cggctttcga aatcttgagg tcgaccacct
      661 tcttctcgcc gtcgatttcg atccggtcac cattctggat gatcgcgatc ggtccgccga
      721 caaacgcttc gggtgtcaca tgcccgatga caaacccatg actgcctccg gagaaccttc
      781 catcggtcat cagggcgaca tcttttccga ggcctttgcc catgatcgcg gacgtcggtg
      841 ccagcatctc gcgcattcca ggtcctcctt taggaccttc gtaacgaatg acaataacgt
      901 ctcctttccg aattttgtcg tctaggattg cccgaagcgc tttttcctcg gactcaaaga
      961 ctctggcctt gcccttaaag cgcatccctt ctttgccgga gattttggcg atggcgccct
     1021 ccggggccag attcccgtag agcacaacca ggtggctgct tgacttgatc ggatcctcga
     1081 acccgcgaac gatcgtctgc cctttcggat agcc
//
LOCUS       DATMKH010000582         6073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4111275, whole genome shotgun sequence.
ACCESSION   DATMKH010000582 DATMKH010000000
VERSION     DATMKH010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 6073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6073
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4111275"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..1129
                     /gene="hpnA"
                     /locus_tag="VNV63_06945"
     CDS             137..1129
                     /gene="hpnA"
                     /locus_tag="VNV63_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid-associated sugar epimerase"
                     /protein_id="HXC62396.1"
                     /translation="MQRTILITGATGFVGAAVVRRLLEAGDGVKALVRKNGDLRNLDG
                     LKLELVYGDLRDKDSLRIAMKGCQRLYHVAAYYSLWTPDARILYECNVDGTQNILEVA
                     KELELEKIVYTSTVGAIGIPKEGGPGNEETPVALDKIIGHYKRSKYLAEQVALRMARQ
                     GLPVVIVNPSAPIGPRDIKPTPTGKMIVDFLKGFMIAYIDTGMNLVDVDDVAAGHLLA
                     AEKGRIGEKYILGHRNMTLKEISDLMSRISGVPAPKFKIPREAVLPFAYINQWISDYI
                     TKKPPLIPLDGVRMAKEYMHYDSTKAIKELGLPQTPIEIAMEKAIRWFKDHDYV"
     gene            1139..1924
                     /locus_tag="VNV63_06950"
     CDS             1139..1924
                     /locus_tag="VNV63_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HXC62397.1"
                     /translation="MSTLLLLISTILYRPYVFIFLLIFLVVSIHVMGWKRTFVFTLIT
                     WAVAFSAEWTSTRFGIPFGDYYYTENTRLIELFISNIPFMDPLSFTFLLYASNTLALC
                     LVLPITKTQDGIALADDLERRHSISAILISAFLFAWIDVVIDPLALRGDRWFLGKIFG
                     YPHGGIYFGVPISNFIGWSVVGLVSLTLFSGVDWFLITQREETLAYLRNSRIKVLGGV
                     FLYYIVLIFNLVMTFYIGEPLIGTVGIFIQLPIFVLLVLKFLG"
     gene            complement(1927..3153)
                     /gene="glgC"
                     /locus_tag="VNV63_06955"
     CDS             complement(1927..3153)
                     /gene="glgC"
                     /locus_tag="VNV63_06955"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017097990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HXC62398.1"
                     /translation="METVEKTLTIILAGGTGSRLHPLTADRAKPAVPFGGIYRIIDFA
                     LSNCLHSGLRQILVLTQYKSHSLQKHLRDGWSIFNPEIGNYITTVPAQMRTGEGWYAG
                     TADAIKQNRYLLERSMAKYVLVLMGDHIYRMDYARMLEEHVLRKLDVTVACVPVPLSE
                     ARFFGVVEIDEDLLVRKFVEKSENPSLMPNDPGYALASMGIYVFNLPFLLEVLALDHR
                     TTTPRHDFGMHILPDIVGTHTVGAYRFGVVEEGRVAPDGYWQDVGTLDAYYEANMDLL
                     KPIPPINLYQHDWPIRTYHGQYPPARNVPSSSGQLGSVTNALLAGGDVIIGAQISHSI
                     LSPDVWVDSGAVVEDSILFERVRVGCDARLRRCIIDKDVQIPPKETIGWDLERDRRRF
                     TMSEGGVVVVPKGYRF"
     gene            3698..5014
                     /locus_tag="VNV63_06960"
     CDS             3698..5014
                     /locus_tag="VNV63_06960"
                     /inference="COORDINATES: protein motif:HMM:NF016830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HXC62399.1"
                     /translation="MIILSGLTVHMLMLIQEPPGWAQDESALKETDEPWDLHFQATVV
                     SQWHPAFPAKYSGPNSLSAKPEFSTSLTSTLFAGARLWHGAEFYINPELVAGDGFSHT
                     LGVAGFPNGEIYRVSDPVPTLFVARLFLRQTFSLGGERERVEPDQNQLGGIYDQHRIT
                     LTLGKFSLTDLFDDNAYSHDPRTQFLNWSIMDNGAWDYAADTRGYTWGVALEYQQSRW
                     AFRLATVMVPKQANKESLDFALSRAHGDNAELEYLYAIQEHPGKLWLSAYANHAHMGS
                     YRDTIDAGVTDITLTRQYRVKYGFGLNIEQELNHDVGVFLRLGWNDGRTETWAFTEID
                     QTASFGISLKGSRWKRPEDTVGIAAAINGLSADHAAYLKAGGVGFIVGDGHLTYAPEQ
                     IIETYYLWKPLPRLGVTVDAQFVNHPAYNMDRGPVYILGSRLHYAF"
     gene            complement(5192..5575)
                     /locus_tag="VNV63_06965"
     CDS             complement(5192..5575)
                     /locus_tag="VNV63_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62400.1"
                     /translation="MNGWMKIALGAFLLVAFTLLERPAMAAPDVNEAKKQSTEMVKNA
                     QQMVEHGNAAHPDVMTKHAKAMIAAAQKTLDNIPPGNMHGETAAGHIKTAIEHAKAVI
                     SEQSVDSAEQALNHAMEGDQHIQQM"
     gene            complement(5755..>6073)
                     /locus_tag="VNV63_06970"
     CDS             complement(5755..>6073)
                     /locus_tag="VNV63_06970"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HXC62401.1"
                     /translation="YRKLNRTAEENRKDSRIPYVRPVSYAVIGQFLHASCEVDLQGII
                     LDISGGGFRIRTDVQPDLEEMMIRAWIPLPQSPFTISVIAEVRWVRDEMQNVYDIGFR
                     FLL"
BASE COUNT         1464 a         1577 c         1560 g         1472 t
ORIGIN      
        1 ttctttggag tggagcggtc aagacagaag gcgtatgatt tgatgaagcg tgggatcaaa
       61 agtctggagc ggttcggcaa gaaagcggac cagctcagag aaatcgcacg ctatatcgtg
      121 gatcggagga agtaggatgc agcgaacaat actaatcaca ggggctacag gatttgtggg
      181 tgctgctgtg gtgcgtcgcc tccttgaggc gggcgacggt gttaaagcct tggtacgtaa
      241 aaacggcgat cttaggaacc tggacggttt aaagttagag cttgtttacg gtgatctgcg
      301 ggataaagat tcactccgca ttgcgatgaa gggatgccaa cggctctacc atgtcgcggc
      361 ctactatagt ctctggactc cggatgcaag aattttatac gaatgcaacg tagacggcac
      421 acagaacatc ctggaggtgg ctaaggagtt ggaactcgag aagatcgtct ataccagcac
      481 cgtaggggcc ataggaattc ctaaagaagg cggaccgggc aacgaagaga cgcctgtagc
      541 gcttgataag atcatcggtc actacaagcg ttccaagtat cttgccgagc aggttgccct
      601 gcggatggcg aggcaaggac tgccggttgt aatcgtgaat cccagcgccc cgatcggtcc
      661 ccgggacatc aagcccacgc ccaccggaaa gatgatcgtg gacttcttaa aaggcttcat
      721 gattgcctat atcgataccg gcatgaatct ggtggatgtg gatgacgtgg cggccggcca
      781 tctcctcgca gccgagaagg gacggatcgg cgagaaatat attctgggcc accgcaatat
      841 gaccttaaaa gaaatatccg atctcatgag ccggatctcc ggtgtccctg cacccaagtt
      901 taaaatcccc cgtgaagcgg ttctcccgtt cgcgtatatc aaccaatgga tctcggacta
      961 tatcaccaag aagcctccgc tgatcccgct ggacggcgtc agaatggcaa aggaatacat
     1021 gcattatgac tccaccaaag caattaagga gctgggtctg ccacaaactc cgattgagat
     1081 cgcaatggag aaggcgatta ggtggttcaa agaccatgat tatgtataat tagttctgat
     1141 gtccacactt ctcctcctca tctccaccat cctctatcgt ccctatgtct ttatcttcct
     1201 cctcatcttt ctggtcgttt ccatccatgt gatggggtgg aagcggacct ttgtctttac
     1261 actgatcacc tgggcggtgg cgttttctgc agagtggacc tccacacggt tcggcatccc
     1321 gttcggtgac tactactata ccgaaaacac gcgacttatc gaactcttca tcagcaacat
     1381 cccattcatg gatcccctgt cgttcacctt cctgctctac gcaagcaaca ctctggcact
     1441 ttgtctggtc ctaccgatta cgaagaccca agacggcatc gcgcttgcgg atgacctgga
     1501 acgccggcac tcgatttccg ccattctgat ctcggccttc ctctttgcat ggattgatgt
     1561 ggtgattgat cccctcgccc ttcgcgggga ccgctggttt cttggaaaga tctttggata
     1621 tcctcacggc ggaatctatt tcggcgtgcc gatctcgaac ttcatcggat ggagcgtggt
     1681 gggactggtc agcctgacgc tcttttctgg agtggattgg ttcctaatca ctcagagaga
     1741 ggagactctg gcctatcttc ggaactcccg gatcaaggtc ctcggaggag tcttcctcta
     1801 ctatattgtg ttgatcttca atctcgtgat gaccttctac atcggcgagc cgcttatcgg
     1861 gacggtaggg atcttcatcc aactccccat cttcgtattg cttgttctta aatttctcgg
     1921 ttgaaattag aatcggtatc ctttgggaac cacgaccacc ccgccttcgg acatggtaaa
     1981 tcggcgacgg tcgcgttcaa gatcccaccc gatcgtctct ttcggaggaa tttggacatc
     2041 tttatcgatg atgcagcgcc ttaaacgggc atcgcagccg acacggactc gttcgaacag
     2101 gattgagtct tcgaccaccg caccagagtc gacccaaaca tccggagaaa gtatcgagtg
     2161 gctgatctgc gcgccgataa ttacatctcc accggccagc aaggcgttcg tcaccgaccc
     2221 aagttgtccc gaagaagatg gaacattacg tgccggcgga tattgaccat ggtaagtccg
     2281 gatcggccaa tcgtgctgat acagattaat gggcgggatt ggtttgagaa ggtccatgtt
     2341 cgcctcataa tatgcatcca gggttccgac atcttgccaa tagccgtccg gcgcgacccg
     2401 tccttcctcc acaacgccaa aacggtacgc tccaacggtg tgtgtcccaa cgatgtctgg
     2461 aagaatgtgc atcccaaaat catgccgggg agtggtggtg cggtgatcta gagccaggac
     2521 ttcgagaaga aatggaagat taaaaacata aattcccatg gaagccagtg catagcccgg
     2581 atcattcggc atcaaagagg gattttcaga tttttcaaca aactttctga ccaagagatc
     2641 ttcatcgatc tccaccactc cgaagaatcg ggcttcagac agtggaaccg gcacgcaggc
     2701 tacggtcaca tctaacttac ggagcacatg ctcctccaac atccgtgcat aatccatacg
     2761 gtagatgtgg tctcccataa ggaccaagac atacttggcc atgctccgct cgagaagata
     2821 ccggttttgt ttgatcgcat cggctgttcc tgcgtaccat ccctccccgg tccgcatctg
     2881 ggccgggacg gtggtgatat agttgccaat ctcaggattg aagatcgacc agccatccct
     2941 caaatgtttt tgaagagagt gcgatttata ctgagtgagg accaatattt ggcgaaggcc
     3001 ggaatgcaga caattggaaa gggcaaaatc aataatccga taaatccccc caaaagggac
     3061 cgccggctta gctcgatcag cagtcaacgg gtgaaggcgt gatccagttc ccccggccaa
     3121 aataatcgtt agggtcttct caaccgtctc catagtaaac tctttccagt gatttgaaaa
     3181 ctggaccctg cttcgtcagc ttacaggaaa aaattttgaa tttcaatgaa aaatgtggct
     3241 gtgaaatcaa gagacgctag agaaacttat ccggaaaggt taaatggggt agagtggaaa
     3301 ggaaagtagg cggggcgctt atgccagcgc aatgcgagtc ccatttactc atgtcacgct
     3361 tcatggaaaa tcatgtaaca ctgtaatgaa ataaaaaaca gcaagaaaaa aattgagatt
     3421 cactgttgat cttgctatat taacaaagat ttttataaca aaatagataa aatcactatg
     3481 cggattctcc tctgtgaaga tgataagaaa ccaggcaagc acatcgagca gacgctcaga
     3541 gcagagggat cggtgttctt ccattacctg gcgcgtactt tatttggctt gtcattattc
     3601 tactctgcta tagtcttctg acgcaggcca tgaagatatg gtttgtcagg aagttcgaga
     3661 gctggttatg agatcaggat tatggagacg cagcgttttg ataattcttt cgggactgac
     3721 agttcatatg ctcatgctga tccaagagcc cccgggttgg gcccaggacg aatcggcact
     3781 caaggagacg gatgaaccct gggacctcca cttccaggca acggttgtaa gtcagtggca
     3841 tccggccttt cctgcgaagt attctgggcc caacagcctc tccgcgaaac ccgagtttag
     3901 cacgtcgctt accagcacgc tgttcgctgg cgcgcgcctc tggcacggcg ccgagtttta
     3961 tatcaatccc gagctcgtgg ccggagatgg ttttagccac acactcggtg tggcaggttt
     4021 cccgaacggt gagatctacc gggtctctga cccggttccg acgttgttcg tggcgcggct
     4081 gtttttgcgc cagacattta gcctgggcgg cgagcgcgaa agggtcgagc cggaccaaaa
     4141 ccagctcggt gggatttatg accagcatcg gatcacgctg accctgggca agtttagcct
     4201 taccgaccta ttcgatgaca atgcctatag ccatgatccc cgcacacagt ttctgaactg
     4261 gtcgattatg gacaatggcg cctgggacta cgctgcagat acccgcggct atacgtgggg
     4321 ggtagcgctg gagtaccagc aatcccgatg ggcgttccgt ctggccacgg tcatggttcc
     4381 caagcaagcg aacaaggagt cactcgattt tgctctctca agagcccatg gagataacgc
     4441 cgaactcgag tatctctatg cgatccagga acaccctgga aaactctggc tatcggccta
     4501 cgccaatcat gcccacatgg gtagctatcg cgatacgatt gacgcaggtg tgacggatat
     4561 tacgctgaca cggcaatatc gcgtgaagta tggcttcggg ttgaatatag agcaagaact
     4621 aaaccatgac gttggcgtct tcttgcgcct cggctggaat gatggcagga ctgaaacttg
     4681 ggcatttaca gagatcgacc agacggcttc tttcggtatc agtctaaagg gatcccgctg
     4741 gaagcggccg gaagataccg ttggaatcgc ggcggccatc aacgggctgt cagcagatca
     4801 tgccgcttat cttaaggctg ggggtgttgg tttcatcgtc ggagatggcc atctgaccta
     4861 tgctccggag cagataattg agacctacta tctgtggaag ccgttaccga ggcttggggt
     4921 cacagttgac gctcagttcg tcaatcaccc ggcctacaac atggatcgcg gcccggtgta
     4981 tatcttggga agccgcctac actatgcgtt ttgagccgaa cacaaataaa tgcaagcgtg
     5041 aagtagatga cacaagatac cccgttgttc cgattggcgg gataaggcct gatcgatcgc
     5101 gccggtattg tgaccgcgtt ggtaccacgg ccccttccga tcccgctttc ttgcgcattc
     5161 caggactgcc gatcgataag cacaagattt tttacatctg ctggatgtgc tgatcacctt
     5221 ccatggcatg gttcaaggcc tgttccgcgc tatctacgct ctgttcgcta atgactgcct
     5281 tggcgtgttc aatggccgtt ttgatatggc ctgcagccgt ttccccatgc atgttacccg
     5341 gcggaatatt gtctaacgtc ttctgggcgg ccgcgatcat cgccttcgcg tgcttggtca
     5401 taacatcggg atgcgctgca ttgccatgct ccaccatctg ctgggcattc tttaccattt
     5461 cagtagactg cttcttggcc tcattcacat ccggagcagc catggcaggc cgttccagca
     5521 atgtgaacgc cacgagcaag aaggcgccta aagcgatctt catccatccg ttcataacct
     5581 tcacctcctt ttagtgttta gctacctgtt cacggcagat tgatactcca cggatggcac
     5641 ctatcttata ttaccgctcc tataggaatc aagtggcgcg agaaaggttt ttttgaaagg
     5701 ttaaatgggc cggaacaggg ctgagaactt tcctggcatc aagatcttct ctccctagag
     5761 cagaaaccga aatccaatgt cataaacgtt ctgcatttca tctctcaccc accgcacctc
     5821 tgcaataact gatatggtga aaggagactg gggcagcgga atccatgccc ggatcatcat
     5881 ctcctccaga tcgggttgga catccgtacg aattctgaag cccccaccgc tgatgtccaa
     5941 gattataccc tggagatcaa cctcgcatga agcatgaagg aactggccta tcaccgcata
     6001 gctcacaggg cggacataag ggatgcgact atcctttctg ttttcttctg ccgtccgatt
     6061 taatttgcgg tat
//
LOCUS       DATMKH010000583         1384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_275693,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000583 DATMKH010000000
VERSION     DATMKH010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275693"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            647..>1384
                     /locus_tag="VNV63_06975"
     CDS             647..>1384
                     /locus_tag="VNV63_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62402.1"
                     /translation="MLDIRRAVLVLFILLTPAIGWSQTQLADNSNLPDDGQQSSTSQD
                     NQTPAPLPDKPVPVPGQTTESQNKKDDSEGKQTKRMLWVVPNFGAVDANKQLPPLSIG
                     GKFKLAAQDSVTDYSSYTWAGILAGQAMLLNSDPELGHGIKGYGRYYWRNFVDGISGT
                     FFTEAIVPAMTHEDPRYYTMENGGFFRRTGYAISRAFVTKTDSGGTSFNWSEVAGNGL
                     EAALSNAYYPPQERGLSQTARNWGTQME"
BASE COUNT          336 a          371 c          364 g          313 t
ORIGIN      
        1 tgctgattga tgccggagcg gatggcatcc gcgtcacttt gctgtttctt gcacccggcg
       61 aagagcgcta gaagcccgag agcggtaaaa aagcatgcac tgcgcttcat gcgagtctcc
      121 tcttccttac ctatatccca ttccaagcac aacgacaatg tgtaggatcg cgagaagagc
      181 aaacgagctt ggtttgtcac cagggagcag gccgaagctt ttgcaagcga tccgttgaac
      241 acggtctatc acgcagacct tgcccacctc tgttcgaagt gcggtcttta tcatctctcc
      301 aagctggaat ggctggagcc ttccttcagc acgcaagatg taaatttctt atcagggtgc
      361 gggatcaaag ataacttgag atgtgctgat tgcagaagag agtttcagga gaatgtcgaa
      421 tttttgaccc tgcgggacgg cacgatggtc cacgaagaat gcatgccgta agcctgccca
      481 tcactaaatc gtcgaaagtg aagccgggca ttgacccgag agacgcccat aacgcggcga
      541 tgtttctctt ggccgaattt cgtcattcat tttaagataa ctaaatctca ggcgcttgta
      601 gtatcgtaaa ttgatcggcc cctagcccga gatttggaga aaccccttgc tcgatatccg
      661 gagggccgtc ttagttctct tcattcttct tacccccgcc atcggctggt cgcagaccca
      721 gctcgctgac aactccaatc ttcctgatga cgggcagcaa tcctccacat cgcaagacaa
      781 ccagactccc gcgcctcttc ctgacaaacc ggttcccgtg ccaggtcaaa ctacggaaag
      841 ccagaacaaa aaggacgatt cggagggtaa acagacgaag cgaatgctct gggtcgttcc
      901 caattttggg gcggtggacg ccaataagca acttccgccg ctatccatcg gagggaaatt
      961 caaactcgct gcgcaagata gcgttacaga ctattcctct tacacctggg ccggaatcct
     1021 ggctgggcaa gccatgctct taaattccga tcccgaactg ggtcatggga tcaagggata
     1081 tggtcgctat tattggcgta acttcgtgga tggcatctcg gggacatttt tcactgaggc
     1141 catagtcccg gcgatgacgc acgaagaccc gcgctattac accatggaga atggagggtt
     1201 ttttcgacga acgggttacg cgatatcccg tgccttcgtc actaagacag actccggggg
     1261 cacaagcttt aactggtctg aagtagcggg taatggactg gaagcggccc tttccaacgc
     1321 ctattatccg cctcaggagc gcggcctgag tcagacggcg aggaactggg gaacgcagat
     1381 ggaa
//
LOCUS       DATMKH010000584         1477 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3148302, whole genome shotgun sequence.
ACCESSION   DATMKH010000584 DATMKH010000000
VERSION     DATMKH010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1477
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3148302"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1477)
                     /locus_tag="VNV63_06980"
     CDS             complement(<1..>1477)
                     /locus_tag="VNV63_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HXC62403.1"
                     /translation="VINRNNRLKRLLELKAPSVSIRNEKRMLQEAVDALFDNGRRGRA
                     IRGPNKRPLKSLSDMLKGKQGRFRQNLLGKRVDYSGRSVIVVGPELKLHQCGLPKKMA
                     LELFKPFIFQKLEERGYVTTIKSAKKMVEKEKPEVWDILEGVIKEHPVLLNRAPTLHR
                     LGIQAFDPILVEGKAIKLHPLVCAAFNADFDGDQMAVHVPLSVEAQIEARVLMMSVNN
                     ILSPANGKPIMVPSQDMVLGCYWLTKGRVGAKGEGKIFSNPEEVRIAYDAKTVEEHAK
                     IKVRINSHLVETTVGRIILGEILPLHVPFSLVNTEMTKKEVIKLIDTCYRESGHQETV
                     VLLDKIKDLGFYYATKAGISICIDDMHIPTRKAELIEKAQREVMEIERQYADGLITNG
                     ERYNKVIDIWAHVTELVASEMMKELGGNGEVAKKTNAKPERREFNSIFMMADSGARGS
                     TQQIRQLGGMRGLMAKPSGEIIETPITANFREGLTVLQYFIS"
BASE COUNT          312 a          437 c          349 g          379 t
ORIGIN      
        1 gagataaagt actgtaatac ggttaagccc tcccggaagt tggccgtgat cggggtctcg
       61 atgatctcac ctgaaggttt ggccatcaaa ccgcgcatcc cgcccagctg ccggatctgc
      121 tgcgtgctcc cccgggcgcc ggagtcggcc atcataaaaa tcgaattgaa ttctctccgc
      181 tcgggtttcg catttgtttt tttcgctacc tcgccattac cacccaactc cttcatcatc
      241 tcgctcgcga ccaactcggt tacatgggcc cagatatcaa tgaccttatt gtaacgctca
      301 ccgttggtga tcaagccatc cgcatattgt cgttcaatct ccattacctc acgttgcgcc
      361 ttctcgatca attcggcctt ccgtgtgggg atgtgcatat catcgatgca gatcgagatc
      421 ccggccttgg tcgcgtagta aaatcctaag tccttgatct tgtcaaggag caccaccgtc
      481 tcttgatggc ccgattctcg ataacaggta tcgatcaact tgatgacttc cttcttggtc
      541 atctccgtat tcaccaggga aaacggcaca tgcagaggca gaatttctcc aaggatgatt
      601 cggcccacgg ttgtttcaac caggtggctg ttgatacgga ccttgatctt ggcatgctct
      661 tcaacagttt tggcatcgta ggcgattcgg acctcttccg gattggaaaa tatttttcct
      721 tctccctttg cccctacccg acccttcgtc aaccaatagc atcccaggac catgtcctgg
      781 gaaggcacca tgatcggctt cccattcgca ggcgatagaa tgttattgac ggacatcatc
      841 aagacacggg cctcaatctg cgcctccacc gaaagcggca catggacagc catctgatcg
      901 ccgtcaaaat ccgcgttaaa ggctgcgcaa acgagaggat ggagtttaat cgccttccct
      961 tccaccaaga tgggatcaaa cgcctggatg ccgagacgat gcagcgtggg cgcccggtta
     1021 agcaaaacag gatgctcttt gattacacct tccaggatat cccaaacctc cggcttttcc
     1081 ttctccacca tcttctttgc gctctttatc gtagtgacgt atccccgctc ttccagcttc
     1141 tggaagatga acggcttgaa aagctccagc gccattttct tagggagacc gcactgatgc
     1201 agtttgagtt ccggcccgac tacgataacc gagcgaccgg agtagtccac tcgttttccg
     1261 agcagatttt gtcggaaccg gccctgtttt cccttgagca tatcgctgag agattttagc
     1321 gggcgcttgt tgggcccgcg gatggcacgg ccccggcggc cattatcaaa gagcgcatcc
     1381 accgcttcct gaagcatccg tttctcgttc cggatactca cgctgggagc cttgagctcc
     1441 agaaggcgtt tcagccgatt gttccggttg atcaccc
//
LOCUS       DATMKH010000585         1314 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3464981, whole genome shotgun sequence.
ACCESSION   DATMKH010000585 DATMKH010000000
VERSION     DATMKH010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3464981"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(205..348)
                     /locus_tag="VNV63_06985"
                     /pseudo
     CDS             complement(205..348)
                     /locus_tag="VNV63_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002629760.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
     gene            complement(516..972)
                     /locus_tag="VNV63_06990"
                     /pseudo
     CDS             complement(516..972)
                     /locus_tag="VNV63_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896618.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(972..>1314)
                     /locus_tag="VNV63_06995"
     CDS             complement(972..>1314)
                     /locus_tag="VNV63_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012412573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXC62404.1"
                     /translation="DTQLAQVFQNLVGNAIKYRSAEVPQVHVSATKNGGQEWIFSVRD
                     NGMGIDPQYFERIFILFQRLHGQKEFKGTGIGLTICKKILEGLGGRIWVESQPKKGST
                     FYFALPERGGK"
BASE COUNT          297 a          373 c          334 g          310 t
ORIGIN      
        1 cttcggctgc aagcgcaaat cgcgatagcg attgcgaata cccgcccatt cagacagaga
       61 agccattttt tgaaagaggc agctcccgga ctcacctgcc gtgacggcat gtggcgttac
      121 aaggccccga ctacttggcg gcggggagtg tgtagccgct gtaagggtct tcaggaaggc
      181 cacgatctga gcatcgggtc ccttgcatct tggtagaacg tcgcgcgtgt tatagaagtg
      241 gacgacttct tttaagctct tcagataacc gttatgcata taaactttca caaagtcggg
      301 acgcggcctc ttgtccacat ttcgcagcgt agccgtctgg aacttgccgt cgaaagaatc
      361 cgcaaagcgg cctcaatgca agtcatcagc tcctccctcg tgcattgcac agccggaaaa
      421 ccgggtaata gcctgctttt acactgcctt taaacacgat gggacgtgga ggatcaatca
      481 tcaactagca agaagtcgga gtgctttttc tttcatcagc gtgcttcgcg cggcaacttg
      541 accacggaca gccagaaacc gtcgatgctc ttcacgactt tgaggaagcc ttcgaggtca
      601 actggcttgg agatgtaaca gtttgcatgg gattgatagc tccgcagaat atccgcctcg
      661 gaagcagacg tagtcaagat taacgacagg aatgctcttg agtgcctgat cgtccttgat
      721 ctcccccaga acctcccggc cgtccttctt gggcaggttc agatcgagca agatcagatc
      781 cgggcaagga gcaccggagt gctcccccgc gccccacaga aaagccattg cttctgcccc
      841 atccgacgcg acgtgcaggt tgatatgcac cttggcgtct ttgaacgctt cccgcgtgag
      901 ccggacatca ccgggactgt cttcaaccaa gagaacttcc ataggtgccg cgtcggttcc
      961 aatcgattcc atcatttccc acctctttcc gggagggcaa agtagaaggt tgagcccttt
     1021 ttgggttgcg actcgaccca gatcctcccg cccagccctt ccaaaatctt cttgcaaatg
     1081 gtcaatccga ttccggtacc cttgaactct ttttgcccgt gcaaccgctg gaaaaggatg
     1141 aagatccttt caaagtactg agggtcaatt cccatcccat tgtctcgcac ggaaaagatc
     1201 cattcctggc caccgttttt cgtggcagag acgtgtacct gagggacttc ggcactgcgg
     1261 tatttgattg cgttccccac gaggttctga aatacctggg ccaattgcgt atcg
//
LOCUS       DATMKH010000586         1227 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2179911, whole genome shotgun sequence.
ACCESSION   DATMKH010000586 DATMKH010000000
VERSION     DATMKH010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1227
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2179911"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="VNV63_07000"
     CDS             <1..360
                     /locus_tag="VNV63_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015080838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HXC62405.1"
                     /translation="NKDVLEFARQESKKTGASIQISHEPEAAAKGADILYTDVWTSMG
                     QESEAENRRKTFQDYQIHRHLLKLAKPTAWVMHCLPAHRGEEITEEVMEGPQAIVWDQ
                     AENRLHIQKAILEMFLP"
     gene            357..>1227
                     /locus_tag="VNV63_07005"
     CDS             357..>1227
                     /locus_tag="VNV63_07005"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HXC62406.1"
                     /translation="MSAKETKTKKVVLDYSGGLDTSVAIQWLIEAYKCQVIAFCADLG
                     QGEDLQAVKEKALKTGASKVYLKDLKTEWVRDYIFPMLRANAVYEGYLLGTAIARPLI
                     AKAQLDVAREEKAEAVSHGATGKGNDQVRFELSYLALQPEIRIIAPWREWKFRSRNDL
                     IEYARHHGIPVSATKERPYSIDRNLFHISYEGGLLEDPWKEPPEDLYLTTVSPEKAPD
                     QPMYLEVGYKDGNPVSVNGKQMPATQLLTHLNETGGAHGIGRLDLVENRYVGIKSRGV
                     YETPGGTILHTAHR"
BASE COUNT          308 a          334 c          353 g          232 t
ORIGIN      
        1 aacaaggatg ttcttgagtt cgcccggcag gagtccaaga aaaccggcgc ttcgattcag
       61 atatcgcatg agccggaggc tgcggcaaaa ggggccgata tactttatac cgatgtctgg
      121 accagcatgg gccaggaatc agaggctgaa aaccggcgga agactttcca agactaccag
      181 atccatcggc acttattgaa gctcgcaaag cctacggcct gggtcatgca ttgtctgccg
      241 gcccatcggg gagaagagat caccgaagag gtcatggagg gaccgcaggc gattgtgtgg
      301 gatcaagccg aaaaccgcct tcacatccag aaagcgattt tggaaatgtt ccttccatga
      361 gcgcaaaaga aaccaagacc aaaaaagtcg tgctggacta ctccggcgga ctggacactt
      421 ctgtggccat ccaatggttg atcgaggcat acaaatgtca agtcatcgct ttctgcgccg
      481 atctgggcca gggggaagat ctccaggcgg tcaaggaaaa ggcgcttaag acgggagctt
      541 ccaaggtgta tctcaaggac ctcaagaccg aatgggtccg cgactatatc ttccccatgc
      601 tccgggccaa tgcggtgtat gaaggttatc tcctcggaac cgcgatcgcc cgcccgctga
      661 tcgcaaaagc acagctcgat gtcgcacggg aagagaaagc ggaggcggtg tcccatgggg
      721 caaccggaaa gggaaacgat caggtccggt ttgaactctc ctatctcgca ctgcaacccg
      781 agatccggat tatcgctccc tggcgagaat ggaaatttcg ctcccgaaac gacctaatcg
      841 aatatgcgcg gcatcacggg ataccggtct cggccacgaa ggagcggcct tacagcatcg
      901 atcggaacct ttttcatatc agctatgagg gtggactgct tgaggatccc tggaaggaac
      961 cgccggagga tctctatctc acaacagtat cgccggagaa ggccccggac cagcccatgt
     1021 atcttgaggt cgggtataaa gacgggaatc cggtctccgt caacggaaag cagatgccgg
     1081 caacccagct cctaacgcat ttgaatgaga ccggcggtgc ccatggtatc ggccgcctgg
     1141 atctggtgga gaaccgttat gtgggcatca agtcccgcgg cgtttatgag acccccggcg
     1201 ggacaatcct tcataccgcc catcgcg
//
LOCUS       DATMKH010000587         1312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4112406, whole genome shotgun sequence.
ACCESSION   DATMKH010000587 DATMKH010000000
VERSION     DATMKH010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4112406"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1312
                     /locus_tag="VNV63_07010"
     CDS             <1..>1312
                     /locus_tag="VNV63_07010"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="PtrB; oligopeptidase that cleaves peptide bonds
                     following arginine and lysine residues; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HXC62407.1"
                     /translation="TLVLAGTNPPQAPEKPHEITEVGHTRNDPFFWLREKDSPEVLKY
                     LEAENRYTESALKHLEKLQATLYREMRGRIKESDVSVPEKIDDFYYYSRNETGKQYAI
                     YCRKKGSLDAPEEVLLDENALAQGQKYFRIGTLAVSRDHKLLAYSTDTSGNETYVLRI
                     KSLETGALLPDEIKNCSESFAWANDNKTFFYDQLDDTHRPYKALRHVLGTSVDQDPTV
                     YEEKDDRFFLEISKSRDQRLIFVSVESELSSEVRFLNASQPDAEWTLIRPRENDLLYH
                     VENHGDRFFIDTNEKAKNFKIVEAPVASPGKENWKDFIPYDPEVKIDGVDAFKDYLVI
                     SERSKGLPALRIYNLQSGESHEINFDESAYEVSLDRNPVFDTKVIRINYSSFITPNSV
                     IDYDVVTHQKELRKEKTVLGGYQKSDYASERIFAKAEDGAEIPIA"
BASE COUNT          354 a          342 c          328 g          288 t
ORIGIN      
        1 accttggttc tggccggcac aaatcctccg caggcacccg aaaagcccca tgaaatcacc
       61 gaggtcggtc acacccggaa tgatccgttt ttctggctcc gggagaagga tagtccggaa
      121 gtccttaaat atctggaagc cgaaaatcgt tacaccgaaa gcgcgctgaa acacctcgag
      181 aaactgcagg caaccctcta tcgtgaaatg cgcggccgaa tcaaggagag cgacgtttcg
      241 gtccccgaaa aaattgacga tttttactac tattccagga acgagacagg gaaacagtac
      301 gcgatctatt gccgcaaaaa ggggagtttg gacgccccgg aagaggtact cctggacgag
      361 aacgccttgg ctcaaggcca gaaatatttt aggattggaa ccttagcggt cagtcgggat
      421 cacaagcttc tcgcttactc cacggacacc agcggcaacg aaacctacgt ccttcggatc
      481 aaatcactcg agaccggtgc gttgctgccg gatgagatca agaattgcag cgagtctttc
      541 gcctgggcca acgacaacaa gacctttttc tatgaccagc tcgatgacac tcatcggcca
      601 tacaaggcgc ttagacatgt tttgggaacc agcgtcgatc aggatccgac tgtttatgag
      661 gagaaggatg accgcttttt cctcgagata tccaaatccc gagatcagag gctaatcttc
      721 gtctccgtcg agagtgaact ctccagcgaa gttcgtttcc tgaacgccag tcaacctgat
      781 gcggaatgga cgctgatccg tccgcgcgaa aatgacctgc tctaccacgt cgaaaatcat
      841 ggtgatcgtt tctttatcga taccaacgaa aaggccaaga attttaaaat agtcgaggct
      901 cccgttgctt ctccgggcaa agagaactgg aaggacttca ttccttacga tccggaggtc
      961 aaaattgacg gcgtggacgc tttcaaggat tatctagtca tctctgagcg atcgaaaggg
     1021 ttgccggcac ttcggatcta caatctccaa tctggagaat cgcacgaaat aaactttgat
     1081 gagtctgcct acgaagtctc gctggatcgc aatccggtct tcgacaccaa ggttatcagg
     1141 atcaattata gttcgtttat caccccgaat tcagtcatcg actacgatgt ggtcacccac
     1201 caaaaggagc tccgaaagga gaagacggtg ctcggcgggt atcagaaaag cgactacgct
     1261 tcggagcgca tctttgccaa ggctgaagat ggtgccgaaa tcccgattgc ct
//
LOCUS       DATMKH010000588         1633 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4112995, whole genome shotgun sequence.
ACCESSION   DATMKH010000588 DATMKH010000000
VERSION     DATMKH010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4112995"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="VNV63_07015"
     CDS             <1..236
                     /locus_tag="VNV63_07015"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019916903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HXC62408.1"
                     /translation="IGIAIFPANGTDAEKLLQRADAAMYTAKQTGSGYVVYVSEEVLQ
                     KAPAAGHDPIGIDSQQPESQKPDSKSQGDSGPA"
     gene            481..951
                     /locus_tag="VNV63_07020"
     CDS             481..951
                     /locus_tag="VNV63_07020"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HXC62409.1"
                     /translation="MPWVFILWIHLSAAVIWIGGMAIVSLVVTPVLASREASSQSTEL
                     LRTIGERFSQVSWICIVALIMTGILNLIHLAIPLDVLFTTRLGKLLIVKWSLVAGMIG
                     LSIYNDFIAGPSLRFLLPRDPVYIRVAARMRWSGRITLILGLAVLFFALGLRRL"
     gene            1079..>1633
                     /locus_tag="VNV63_07025"
     CDS             1079..>1633
                     /locus_tag="VNV63_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HXC62410.1"
                     /translation="MDLTNWTILFACGTALAGIGTGLYLMFWVMRLSPGSDRMQQIAL
                     AIQEGAKAYLNRQYRTVGVVAVVLFILLWAAGSISDRFGLLSAIGFLVGASASATAGY
                     IGMLLAVRANVRTTQAAHGGLNAALQVAFRGGAVTGLLLIGLGLLAVAGFYRIAISVT
                     TPDRAFSALLSLGFGSSLISVFARV"
BASE COUNT          329 a          430 c          439 g          435 t
ORIGIN      
        1 gtatcggtat tgcgatcttt ccagccaatg gcactgacgc cgaaaagctc ctccagcggg
       61 cggatgcggc gatgtacaca gccaaacaga ccggcagcgg ctatgtggtt tatgtttccg
      121 aggaggttct gcaaaaagca ccagccgctg gccatgatcc aatcgggata gacagccagc
      181 agcctgaatc acaaaaaccg gactcaaaaa gtcagggaga cagtggaccg gcttaatgtg
      241 atcaccgttt aactaacttc tttcaactga taacccctcc cgggaagttc aggcaggggt
      301 tttatttttt cataatttta aacccctccg gcgagccgga ggggttttta tttgaccgct
      361 aaagataagc aaaccggtag gcctcttgga tcattggcga tgatctcacc gaacatcgcc
      421 taattccttg cattctcgac gatggttgat tatagtgtac acaacaagaa atttctatct
      481 atgccctggg tattcatcct ctggattcat ctttcggctg ccgtgatctg gatcggtggg
      541 atggcaatcg tctcactggt tgtcacaccg gtgctggcgt ctcgcgaagc gtcgtctcag
      601 agcactgagt tgctgcggac tatcggagaa cggttcagcc aggtgagctg gatctgcatc
      661 gtggctctga taatgaccgg cattctgaac ctcatccacc tggcgatccc cctggatgtt
      721 ctttttacca cccgccttgg gaaactgctc atcgtaaaat ggagtctcgt ggccgggatg
      781 atcggtctga gcatctataa tgattttatc gccggcccaa gtcttagatt tctcttacct
      841 cgggaccctg tctatatccg cgttgccgct cgcatgcgtt ggtccggaag aattactctc
      901 atcctgggtc tggcggtgct gttcttcgcg cttggtttga gaagacttta atgcgagcac
      961 ccgcataaat cttttgaaaa tctacaccct tttgtgttaa catgcctcgc ttttggtaag
     1021 tcgctctaag tcgatgcggt aaaggcatct ataaaattta attaagaagg agactttgat
     1081 ggatctgaca aattggacga ttctttttgc ctgcgggacc gctctggccg ggattggaac
     1141 cggtctttac ctcatgttct gggtaatgcg cctttctccc ggctcggatc ggatgcagca
     1201 gatcgctttg gcgattcaag aaggagcgaa ggcttactta aatcgccaat accgcaccgt
     1261 gggggtggtt gcggttgttc ttttcatcct cctctgggcg gccggttcaa tctcagacag
     1321 attcggcctc ctctcagcaa tcggttttct tgtgggcgca tcggcatcgg caacggccgg
     1381 ttatatcggc atgttgcttg cggtgcgggc caatgtccgc accacacagg ccgcccatgg
     1441 gggactgaac gccgccctcc aggtagcctt ccggggcggg gcggtgaccg gtctcttgct
     1501 gatcggtctg ggcctcttgg cagtggcggg attttacagg atcgcgatca gtgtgaccac
     1561 gccggaccgg gcgttttctg ccctgctctc tctcgggttc ggcagcagtc tcatctcggt
     1621 ctttgccaga gtc
//
LOCUS       DATMKH010000589         1712 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_918389,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000589 DATMKH010000000
VERSION     DATMKH010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_918389"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..168)
                     /locus_tag="VNV63_07030"
     CDS             complement(<1..168)
                     /locus_tag="VNV63_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HXC62411.1"
                     /translation="MDKKIVAEILEEIGTLLELRGENPFKSRAYSNAARVIESLEQDL
                     DLLVKSDELGKL"
     gene            complement(161..1153)
                     /gene="queG"
                     /locus_tag="VNV63_07035"
     CDS             complement(161..1153)
                     /gene="queG"
                     /locus_tag="VNV63_07035"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HXC62412.1"
                     /translation="MEPVSDSFTANIKAYARKIGFDLVGITTAEILEEDEQHLAVWLE
                     AGFAGEMAYMARSPSRRARPQELLPDAKSVICLGLYYYPGEPSEPDPDSLLGEVSRYA
                     WGKDYHQVIEHLLDELIAYLKKQTGSALKFKTMVDHGPVLEKALAQRAGLGFIGKNTL
                     LIHQDLGSWVFLAELITNLDLEPDKPQFNQCGSCTACLSACPTGALVQPFVLDARRCI
                     SYLTIELKGEIPESLRPQMEDWVFGCDICQEVCPFNAQQKTIGHPYFTASEGAGPWLS
                     LSDVLAVREDQDFKAKFSETPLLRPKRQGLQRNAAAVAQNLESAGQTAGRNKNG"
     gene            complement(1158..1544)
                     /locus_tag="VNV63_07040"
     CDS             complement(1158..1544)
                     /locus_tag="VNV63_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009382212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HXC62413.1"
                     /translation="MEERIFTLNEANNLLPQLEELLTDTKHLRKFLVSIEGEIKKASG
                     HAVQGGGSPFGPSYLKTLERIIKNIEQTNEMGVLVKDLDKGLCDFAYRMDGRVVYLCW
                     KLGEAEINWWHETNTGFTGRQPLEKE"
BASE COUNT          380 a          483 c          421 g          428 t
ORIGIN      
        1 cagcttcccc agttcgtccg acttgaccag caggtcgaga tcctgctcca gagattcgat
       61 cactctggcc gcattggaat aggcgcggga cttgaacggg ttctcgcccc ttagttccaa
      121 aagcgttccg atctcttcta agatctctgc aacgatcttc ttatccattt ttatttcttc
      181 cggctgtctg tcccgccgat tcgagatttt gagccactgc ggctgcgttc cgttgaagac
      241 cttggcgttt ggggcgcaag agcggcgttt cagagaattt agccttgaag tcctgatcct
      301 cccggacagc aagcacgtcg gaaagagaaa gccacggtcc cgcgccttcg gatgcggtaa
      361 agtacgggtg accgattgtt ttctgctgag cgttaaaggg gcaaacctcc tggcagatgt
      421 cgcagccgaa gacccagtcc tccatctgtg gacgcaaaga ttctgggatc tcgcctttga
      481 gctcaatcgt gagataagag atgcaccgac gagcatccag aacaaacggc tgaactagcg
      541 ctccagtggg gcaggcggaa agacaagcgg tgcagctccc gcattgattg aattgtggtt
      601 tgtccggctc aagatcgaga ttggtaatta gctccgcaag aaaaacccag gagccgagat
      661 cttgatgtat caggagcgtg ttctttccga taaatcccaa cccagcccgc tgggccagcg
      721 ccttttccaa caccgggcca tgatccacca tagtcttaaa cttcaaagcc gaaccggtct
      781 gctttttaag ataagcaatg agttcatcca gaagatgctc gatgacctgg tggtagtctt
      841 ttccccaggc gtagcgcgag acctcaccga gtaaactgtc cgggtccggc tccgagggtt
      901 cgcctggata ataatagagg cctaaacaga tcacgctctt ggcatccggc aatagctctt
      961 ggggccttgc ccgacggctg ggacttcgag ccatataggc catctcgccc gcaaagcctg
     1021 cctcaagcca aaccgccagg tgctgctcgt cctcctccag tatctctgct gttgtgatgc
     1081 cgaccagatc gaaacctatc ttccgggcat aagccttgat atttgctgtg aaactgtccg
     1141 acacgggctc catgctctta ttctttctcc aacggctgac gtcccgtaaa accggtattt
     1201 gtctcatgcc accagttgat ctcggcctcg ccgagcttcc agcagagata aaccacgcga
     1261 ccgtccatcc tgtaggcaaa gtcgcagagc cctttgtcca agtccttgac caggacgccc
     1321 atttcattgg tctgttctat gtttttgatg atccgttcaa gtgtcttcaa gtaactcggt
     1381 ccaaacgggc tcccgccgcc ttgcactgca tgaccactgg ccttcttgat ctcgccctcg
     1441 atgcttacca aaaatttacg gagatgtttc gtatcggtta aaagttcttc gagttgcggt
     1501 aaaagattat tggcctcatt taaggtgaag atccgttctt ccataaatcc ctctctccaa
     1561 cgctctttct tagagtttaa ggctgattga gaccccgtcg cgcagcggga ggatcgtggt
     1621 ccagagattt ttagagccat agagttgctc tgtcaatcgt ttgaccccta aaacatccgg
     1681 ttcagttgag ccggttaaga ccgacccaaa cc
//
LOCUS       DATMKH010000590         1328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_920213,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000590 DATMKH010000000
VERSION     DATMKH010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_920213"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1325)
                     /locus_tag="VNV63_07045"
     CDS             complement(<1..1325)
                     /locus_tag="VNV63_07045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HXC62414.1"
                     /translation="MPDGTADFLSQSWLEYSGQTREEAMGWGWAGALHPDDANRVLAN
                     WQAGLDSREPVEMEFRCRRADGTYRWFLDRNLPLRNDEGKIVKWYGILFDIDVLKKTE
                     SALQMREHELLGIIETIPSMILSISPTGEVTHLNQRHIEYFGARFEELTNGGWAKFIH
                     PDDLEETAKSFFRSIEAGEPYNLINRLRRADGEYRWHHGMGGPLRDPDGKIIQYYGLS
                     VDIDEWKRAEETVRKSERELRTLIDVMPAIVGVSLPDGTVDFLSQSWLDYFGQTTEEA
                     MGGGWASVIHPDDVDRVLANRQAGLASGDLVVQELRCRGADGVYRWFLNRSLPIRDDE
                     GKVVKSYGILFDIDVLKKTESALQMREHELLGIIETIPAMLWALSPTGEPTYLSQRFL
                     EYRGASVEEYANRGWENFIHPDDREANAKAFFRSIETGESFNVIQRYRRA"
BASE COUNT          315 a          391 c          295 g          327 t
ORIGIN      
        1 gcgcgtcgat agcgctgtat cacgttgaaa gactctccag tttcgattga ccggaagaat
       61 gccttcgcgt tcgcctctcg atcatcagga tgaatgaaat tctcccaccc gcgattagcg
      121 tattcttcaa cggaagcccc acgatattca agaaatctct gagagagata cgttggttct
      181 ccggtcggcg acaatgccca aagcattgca ggaattgttt cgatgatgcc aagaagttca
      241 tgctctcgca tctgaagagc actttcagtc ttcttcaata cgtcgatatc gaatagaatc
      301 ccgtacgatt taacgacctt cccttcatca tcacgaatag ggaggctgcg gttcaagaac
      361 cagcggtaga ctccgtcggc accacggcaa cgtagctctt gcacaacaag gtctcctgag
      421 gccaatcccg cttgccggtt tgccaatact cgatcgacgt cgtcgggatg aatcacactc
      481 gcccagcccc ctcccatcgc ctcttctgtc gtctgaccga aataatccaa ccaactctgg
      541 ctcagaaaat ccactgttcc gtcgggcaaa gagaccccca cgattgcggg cattacatca
      601 atgagggtcc gaagctctct ctcgcttttc cggactgttt cctctgctcg cttccactca
      661 tcgatatcca ccgagaggcc atagtactga atgatcttac cgtcgggatc acgcaaaggt
      721 ccgcccatac cgtgatgcca ccggtattct ccatcagcac gtcgcaagcg atttattagg
      781 ttgtatggct ctccagcttc gattgaccga aagaatgact tcgcggtctc ctcaagatca
      841 tctggatgaa taaacttcgc ccacccgcca ttagtgagtt cttcgaagcg agccccaaaa
      901 tattcaatgt gtctttgatt tagatgcgtg acttctcctg tcggcgatat tgacaaaatc
      961 attgaaggaa tggtttcgat aatgccgagg agttcatgct ctcgcatctg aagagcactt
     1021 tcagtcttct tcaatacgtc gatatcgaaa agaatcccgt accacttaac gatcttccct
     1081 tcatcattgc ggagagggag attgcgatcc aggaaccagc gataggttcc atccgcccgc
     1141 cggcaacgaa actccatctc aacaggttct cgtgagtcca atcccgcctg ccagtttgcc
     1201 agtactcggt tggcgtcgtc aggatggagc gcacccgccc agccccatcc cattgcctct
     1261 tctctggtct ggccggagta ctccaaccag ctctggctca gaaaatcagc tgttccatcg
     1321 ggcaagca
//
LOCUS       DATMKH010000591         2722 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3151320, whole genome shotgun sequence.
ACCESSION   DATMKH010000591 DATMKH010000000
VERSION     DATMKH010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3151320"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..618
                     /gene="ribH"
                     /locus_tag="VNV63_07050"
     CDS             148..618
                     /gene="ribH"
                     /locus_tag="VNV63_07050"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HXC62415.1"
                     /translation="MTNKKIEGELSGAGLRFGVVVSRVNEFITSRLLAGTLDALTRSG
                     VRLDDVEIVRVPGAFEIPITAKKMAQMRRYDAIICLGALIRGETPHFDYLSAAVTQGI
                     TQVALESGIPLSFGVLTTNSLEQAIERAGAKQGNKGWEAAIGAIEMANLFRHLK"
     gene            637..1095
                     /gene="nusB"
                     /locus_tag="VNV63_07055"
     CDS             637..1095
                     /gene="nusB"
                     /locus_tag="VNV63_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HXC62416.1"
                     /translation="MGLRRKAREYVLQLLFQQDFSQQKTLLNETFRLYRPAAKLIKDF
                     ADLLVEGVTQHISEIDSLIQKHAEHWSLERIALVDRNILRIAIFELLYLDDIPARVTL
                     NEAIEIAKRYGDESSSAFINGILDKILSEEGRLLTKKVEVQKSLNANQGA"
     gene            1076..1672
                     /locus_tag="VNV63_07060"
     CDS             1076..1672
                     /locus_tag="VNV63_07060"
                     /inference="COORDINATES: protein motif:HMM:NF014808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HXC62417.1"
                     /translation="MQIRALSERVTRIRTEILVLGFFQDVRPVRGLAGEVDWIHNGVF
                     SHLMKLQKLKGQCGEAMLLASEAKLLTPKVLLVGLGPSPSYNDSVLLQVLMDIRNRLA
                     ALKVKRFAMETMGVESRLMEWKPFFDVFLATFKNSEAVDDLDVTLLASGEEEARTMTE
                     QIRPALSTQTHILDPSVQSTGQRHRGGGRQPGLSGRQD"
     gene            1695..>2722
                     /gene="purB"
                     /locus_tag="VNV63_07065"
     CDS             1695..>2722
                     /gene="purB"
                     /locus_tag="VNV63_07065"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HXC62418.1"
                     /translation="MIDRYTRPQMKAVWEPAHKLETWLKVELLICEALAEQGVIPKPA
                     MRNIKKKAVVDLKRASRLEQLVKHDVIAFLTAVAEKVGPEGRFLHLGATSSDILDTSL
                     AILLREAADLLISDLERLRSALKEKAFKHKRTLMIGRSHGIHGEPITFGFKMALWYQD
                     VTRQLVRLKQAKEEVSYGKISGAMGTFAHLDPAVEEYVCRRTGLKPAPISSQIIQREQ
                     HAHFLMVLSLIAGTLEKFATEIRHLQRTEVREVEEPFTDGQKGSSAMPHKRNPIFSEN
                     ITGLARLVRSYALAAMENISLWHERDISHSSVERVILPDSTILLDFMLSRMTEVLEPL
                     VVYPENML"
BASE COUNT          681 a          645 c          753 g          643 t
ORIGIN      
        1 tatctacgta ccaaacggga caagttaggg catatcttaa ataagctgta agggagtcgg
       61 gtaggttttt gttgcaggtc acacgtttca agttaagagg ttctaacgtg aaacttttaa
      121 cttacagcaa gcgaacgtga aatgaagatg acaaataaga aaattgaggg agaattaagc
      181 ggcgcaggcc ttcgctttgg cgtggtggtc agccgagtga acgaatttat aacgagccgt
      241 ctgcttgctg ggacgctgga cgccttaacc cgcagcgggg ttcggttaga tgacgtggag
      301 attgtccggg ttcctggtgc ttttgagatt cctatcacgg cgaagaagat ggcgcaaatg
      361 cgccgctatg atgcgattat ctgtctgggt gccttgatcc ggggagagac gccgcacttt
      421 gattacctca gtgcagcggt gactcaaggg attacgcagg tggccctcga gtccggaatc
      481 ccgctctcgt ttggggtttt gaccaccaac agtctggagc aggccattga acgggccggc
      541 gccaagcagg gaaacaaagg atgggaggct gcgatcgggg cgatcgagat ggcgaatctc
      601 ttccggcact tgaaataaat aattattgga tcaatcatgg gacttcgtcg aaaagcgaga
      661 gagtatgtct tacagcttct cttccaacaa gatttcagtc agcagaaaac cctcttaaat
      721 gaaaccttca ggttgtatcg tcccgccgct aagctcatta aagattttgc cgatcttctt
      781 gtagaggggg tgacccagca tatttcagag attgattctc tgatccaaaa gcatgcggag
      841 cactggagcc tggaacggat agccttggta gatcggaata ttcttcggat tgccatcttt
      901 gaactccttt acctcgatga tatcccggcc agggtaacct tgaatgaggc gatcgagatc
      961 gccaaacggt atggggatga gtcctccagc gctttcatca acggaattct agacaagata
     1021 ctcagcgaag aaggaaggct gctgacgaaa aaagttgagg ttcaaaaaag cctgaatgca
     1081 aatcagggcg cttagtgagc gtgtaacacg aataaggacc gagatcttgg tccttggatt
     1141 ctttcaggat gttcggcccg tccgaggatt ggcgggggag gtggactgga tccataacgg
     1201 cgtcttttct catctgatga aacttcagaa actcaaaggt cagtgcggtg aggcgatgct
     1261 cttggccagc gaagccaaac ttctgactcc gaaggtcctt ctcgtaggtt tgggaccgag
     1321 cccttcttat aacgattctg ttcttctgca agttctcatg gatatccgta acaggttggc
     1381 agctcttaaa gtgaagcggt ttgcgatgga aaccatgggg gtcgaaagcc gtttgatgga
     1441 atggaaacct ttctttgatg tctttcttgc gacctttaaa aattctgagg ctgtcgacga
     1501 tctggatgta accctgctgg cctctggtga ggaagaggcc cggaccatga cagaacagat
     1561 acggcctgcc ttatcaactc aaacccacat acttgatcct tccgtgcaat ctaccggaca
     1621 acgacatcga ggcgggggca gacagcccgg actgtccggc aggcaggatt gacaaaagaa
     1681 ggaagaagaa agacgtgatt gatcgttaca cacggcccca gatgaaggcg gtttgggagc
     1741 ctgcccataa gcttgagacc tggctcaagg tggaactcct gatctgtgag gcgctggcag
     1801 agcagggtgt gatccccaag cccgcgatgc ggaatatcaa gaagaaagcg gtcgttgatt
     1861 taaaacgagc gagccgtctg gagcagttgg tcaagcatga tgtgatcgcc tttctaacgg
     1921 ccgttgcaga aaaagtgggc ccggaggggc gctttctcca tctcggcgcg acctcttccg
     1981 atatcctgga cacaagtctg gcgattctcc ttcgcgaagc ggccgatctc cttatttcag
     2041 atctggaacg ccttcggtca gcactcaaag aaaaagcttt caaacataaa cgcactctga
     2101 tgatcggccg atctcatgga attcacggtg aaccgatcac cttcggattc aagatggcgc
     2161 tatggtatca ggatgtcacg cgccagctag tccggcttaa acaagccaag gaggaggtaa
     2221 gctacggaaa gatttcgggc gccatgggga cctttgccca tttagacccg gcggttgagg
     2281 aatatgtctg tcgtcgaaca ggcctcaaac cggcacctat ctccagccag atcattcagc
     2341 gagaacagca tgcacatttt ctgatggtcc tctcgctgat cgcgggcacg ctggagaaat
     2401 ttgcaaccga gattcgccat ctacagagaa ccgaggtgcg ggaggtggag gagcctttta
     2461 ccgatggcca gaaagggtcc tctgccatgc cgcacaaacg caatcctatt ttctctgaaa
     2521 atatcaccgg tcttgcaagg cttgttcgat cctatgccct tgctgccatg gaaaacattt
     2581 ctctctggca tgagcgggac atcagccact cctccgttga acgggtgatc ttaccggaca
     2641 gcacgatcct gctggatttt atgctgagcc gtatgaccga ggttttggag ccgctcgttg
     2701 tttatccgga aaacatgttg aa
//
LOCUS       DATMKH010000592         1273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4741088, whole genome shotgun sequence.
ACCESSION   DATMKH010000592 DATMKH010000000
VERSION     DATMKH010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4741088"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(133..687)
                     /locus_tag="VNV63_07070"
     CDS             complement(133..687)
                     /locus_tag="VNV63_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012554453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXC62419.1"
                     /translation="MTNDELRALNKKRKIHQIAWVTRDLEKSMRAWVDNLQVGPWTVL
                     TFTEKTLKYLDVDAKRVTEPFKFLIGISWIGEIQVELIQPVYGPMIYEAFLQKHGEGL
                     HHIKERISQDDIDEVLKEYQEKGIEVTQTGQFKEDFHYYLNTEPKLDFVLELGNCPLL
                     NLPKDEFPIYPPDSGDQAHGKSSG"
     gene            complement(1053..>1273)
                     /locus_tag="VNV63_07075"
     CDS             complement(1053..>1273)
                     /locus_tag="VNV63_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62420.1"
                     /translation="VTVTIRLTPDFGSTSFTLMVPQVNLDQSSESHVTTFGVTTFHRV
                     SIAPQLDRGQTEHYTVAELSGTAALVAF"
BASE COUNT          269 a          333 c          341 g          330 t
ORIGIN      
        1 ttcgggtccg acctaaggga atccgagacg agcacaagga cgattgggga acgtttcttt
       61 acgttgttcc tccaagaatc ctttgcgttc ttcgctcgta atcgtctcgt ccgggacgaa
      121 gccctatggt aattagcctg aggatttccc gtgggcctga tcgccggagt ccggcggata
      181 aattgggaac tcgtccttcg gcagattcaa gagagggcag tttcccagtt ccaggacaaa
      241 atccagcttc ggttccgtgt tgagataata gtggaagtcc tccttgaact gtccggtctg
      301 cgtcacctcg atgccttttt cctggtattc cttaagtacc tcatcaatgt cgtcctgact
      361 gatgcgttcc ttaatgtggt gcagcccttc accgtgtttc tggagaaagg cctcgtagat
      421 catcgggcca taaaccggct ggatcaattc tacctgaatt tctccgatcc agctgatgcc
      481 gatcaaaaac ttaaaaggtt cagtcactcg tttggcatcg acgtccaggt atttcagggt
      541 cttctctgtg aacgtgagca ccgtccacgg cccaacctgc agattgtcga cccatgctct
      601 catgcttttt tccaaatctc gcgttaccca tgcgatttga tgtattttcc gttttttatt
      661 gagcgcgcga agttcgtcgt tggtcatttt cgttagagaa tggggggtta gactttgttt
      721 caacaatggc gaatgactga tagagttcga tcagccttgc atcaagcccc aactgcgcca
      781 gcaaaaagga cgtaaagcgt cccgctctgc ctcttcaacc ccggaaggaa aacccaggta
      841 aaccggggac ggttcagtta ccggctgggg aagcaaggcg tccagctcta cctctcgtgg
      901 accagggtcg aatattgtct ggtctcttgt tcttctggcg cgccctgcag ggcgggtcgt
      961 cgcgatgggg agattcccgg cgttaaaacg tcgggctgaa ccctctagcc cccgcgggct
     1021 tgcctttcta gtcgctcatt cgctaaattc tattaaaagg cgaccagggc tgctgtgccg
     1081 gagagttcag caaccgtgta gtgttctgtc tgaccccggt ccaattgagg cgcaatcgac
     1141 actcggtgga atgtcgtaac cccgaaggtg gtgacgtggg attcgctgga ctggtccaaa
     1201 ttcacctgag gcaccattaa ggtgaaggaa gttgagccaa agtcaggggt caagcggatg
     1261 gtcaccgtga cca
//
LOCUS       DATMKH010000593         1330 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4115234, whole genome shotgun sequence.
ACCESSION   DATMKH010000593 DATMKH010000000
VERSION     DATMKH010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4115234"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1281)
                     /locus_tag="VNV63_07080"
     CDS             complement(<1..1281)
                     /locus_tag="VNV63_07080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXC62421.1"
                     /translation="MKDFERLVQDLEVHQTELEAQNEELRTAQVGLEESRHQYSDLYD
                     FSPVGYFSFDPAGLILKVNLTGAGQLGVDRRLLIKKPFHTFVHPDDRDRFRDHLRSVF
                     STEDRHECEVRIQKWGQQKTGVEKDTQPFYAQLGSIFAKDTAGQTSCRTVLSDITLRK
                     RAEEELFNASRMESVGLLAGGIAHDFNNILTVIIGNLSLAKIYLGPEHKAFQKMAESA
                     KAALQGQKLTQQLLTFSKGGKPIRKNILITPIIKQSVNFVLHGSNVRCDFLLPEDLWP
                     TEVDEGQISQVIYNLIINAQQAMPEGGVIQVYGKNIVLGKEEGLPLRPGQYVRIAIRD
                     EGVGIPSENLSKIFEPFFTTKPTGSGLGLATAYGIIKRHDGHLTVESTLRQGTTFCIY
                     LPASGKTILETEAVAETGIAETEAETEAETEAETE"
BASE COUNT          276 a          379 c          315 g          360 t
ORIGIN      
        1 ctctgtctct gcctctgtct ctgcctctgt ctctgcctct gtctctgcta ttcctgtctc
       61 agccactgcc tctgtctcca aaatcgtctt cccggaggca gggagataga tacaaaaggt
      121 ggtcccttgc cgcaatgttg attctaccgt caaatggccg tcatgccgtt tgatgattcc
      181 gtaagcggtt gccaacccta atccgcttcc cgttggcttg gtcgtaaaaa atggctcaaa
      241 gatcttgctg agattctcag agggaatccc gactccttca tccctgatcg cgatcctgac
      301 gtattgaccg ggtcgtaacg gcaggccttc ttctttgccc agcacgatat ttttgccata
      361 gacctgtatt actcccccct ccggcatcgc ttgctgggca ttgatgatca gattataaat
      421 cacctggctg atctgtcctt cgtccacttc cgttggccac aggtcctccg gcaacaaaaa
      481 atcacacctc acattggagc cgtgcaacac aaaattaacc gattgtttga ttatgggagt
      541 gatcagaata tttttcctga tcggcttgcc gcctttggaa aaagtcaaca gttgctgagt
      601 gagtttctgc ccctgcaggg cggcctttgc ggattcggcc atcttctgga aggccttatg
      661 ttccggccct aaatagatct tcgccagaga caaattcccg atgatcactg tcagtatatt
      721 gttaaagtcg tgggcaattc cgccggctaa cagaccgacc gactccatcc ggctggcatt
      781 gaacaactct tcctcggccc gtttgcgcag tgtgatgtcg ctgagcacgg ttcggcagga
      841 tgtctgaccg gcggtatcct tcgcaaagat gcttcccagt tgggcataaa aaggctgtgt
      901 gtctttctcc actcccgttt tctgctgccc ccacttctgt atcctgactt cgcattcatg
      961 ccggtcctct gtgctgaaaa cggaccgaag atgatcccga aaacgatccc ggtcgtcagg
     1021 atggacaaaa gtatgaaacg gttttttgat caacagacgc cggtccacgc ccagttggcc
     1081 ggccccggtc agattgacct tgagaatcag cccggcagga tcaaaagaaa aatatccgac
     1141 cggtgagaaa tcgtagagat cagaatactg atgtcgcgac tcctccagcc cgacttgggc
     1201 ggtccggagt tcttcgttct gcgcctccag ctctgtctga tgcacttcca agtcctgtac
     1261 aaggcgctcg aaatctttca tggtaggatt cggctgggct tgtaatttct tgcggacttt
     1321 cgcctgcttc
//
LOCUS       DATMKH010000594         1044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_597759,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000594 DATMKH010000000
VERSION     DATMKH010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_597759"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..119)
                     /locus_tag="VNV63_07085"
     CDS             complement(<1..119)
                     /locus_tag="VNV63_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HXC62422.1"
                     /translation="MIGRNRYKNRIELLQGTLDMLILQTLQWGPHHGYGIVQAL"
     gene            complement(226..>1044)
                     /locus_tag="VNV63_07090"
     CDS             complement(226..>1044)
                     /locus_tag="VNV63_07090"
                     /inference="COORDINATES: protein motif:HMM:NF025847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4436 family protein"
                     /protein_id="HXC62423.1"
                     /translation="ATDDNFVIANVKITSVDTAQGLLHGRIRLIPMGRFAIDKTTPAT
                     NLTLLVNSISGKQKTVFPKGERIVPIDFSILLSGSQSRYPFDRYTTDIELVITTPAKK
                     KSEPPPDDTLDENADPLATTLIVGASDLDNSEPVPIKENFVASIPGIKFAGTITENST
                     YKLMQTAVEMRRANNVISISLMVMGLMFVLAISIVRMVLTVTASPGAMNLLPLSLSIT
                     LIFGLPALRSIQPGVPPVGGFSDYMSFIWAEMMVSISAIALAWTWIIRSKKDGN"
BASE COUNT          235 a          245 c          305 g          259 t
ORIGIN      
        1 agggcctgca cgattccata gccgtggtgg ggtccccact gcagcgtttg aaggatgagc
       61 atgtcgagtg tgccctgcag gagctcgatg cggttcttgt agcgatttct tcctatcatg
      121 atgtaggcac tctacacctg atggaggtag agtgtctacg gcattggagg cccaacatga
      181 agcccggcgc agatcgggga cctcggatct gcagacctga ggcgtttagt ttccgtcttt
      241 ttttgaacga attatccagg tccaggccaa tgcgatggct gagatcgaga ccatcatttc
      301 ggcccagatg aaagacatat agtcgctgaa gccgcctact ggaggtacgc cgggctgaat
      361 gctccgcagc gcgggaagac caaagatgag ggttatagag agggagagag ggagcaggtt
      421 catcgcaccc ggagatgcag tcacagtcaa gaccatcctc acaatgctga tagccagtac
      481 aaacataagc cccataacca tcaaggaaat gctgattacg ttatttgctc ttcgcatttc
      541 gacagcggtc tgcatgagct tgtaggtgga gttctcggta atcgttccgg cgaacttgat
      601 tcccggaatt gaagcgacga aattttcctt gatgggtacg ggttcactgt tatcaagatc
      661 gctcgctcca acgatcaggg tggtcgcaag tggatcggcg ttttcgtcga gagtatcgtc
      721 aggcgggggc tcggacttct tttttgccgg cgtggtgatc accagttcga tatccgtcgt
      781 ataacgatca aacggatacc tgctttgact tcccgagaga agaatactga agtcgattgg
      841 aacgattcgc tcgcctttgg gaaagaccgt cttttgtttt ccggagatcg aatttaccag
      901 gagcgtcaga ttggtggccg gagtggtttt gtcgatggca aatcgaccca tggggatgag
      961 cctgatacgt ccatgcagca atccctgcgc ggtgtcgacg gacgttatct tgacgttggc
     1021 tatgacgaaa ttgtcgtctg tggc
//
LOCUS       DATMKH010000595         1055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2846830, whole genome shotgun sequence.
ACCESSION   DATMKH010000595 DATMKH010000000
VERSION     DATMKH010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2846830"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..928
                     /gene="prfB"
                     /locus_tag="VNV63_07095"
     CDS             <1..928
                     /gene="prfB"
                     /locus_tag="VNV63_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_155970262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HXC62424.1"
                     /translation="MKQWEELEKRSEDLFLMHELVKEESDASGLEELGRDIEKFQKDI
                     DQLEIQLILSGEKDLNNAIITIHPGAGGTESQDWAQMLMRMYLRWAEKEGFKTELLDL
                     QAGEEAGIKDATFSVIGEYAYGKLKAEAGVHRLVRISPFDANKRRHTSFASVFVYPEI
                     EDDAELELDEKEIKMEAFRSSGPGGQNVNKVSSAVRLTHIPTGIIVACQTERSQHKNR
                     SVAMKLLRAKLYEVEKEKKEAEMNRLVSASGGAKKEITWGSQIRSYVFQPYQMVKDHR
                     TNLEIGNVNAVMDGEIDPFIQAFLLQSIKSNR"
BASE COUNT          264 a          286 c          291 g          214 t
ORIGIN      
        1 gatgaagcag tgggaagagc ttgagaagcg atcggaagat ctcttcctga tgcatgagct
       61 cgtcaaggaa gaatcggacg cgtccggcct ggaagagctc ggacgggaca tcgaaaaatt
      121 tcagaaggac atcgaccagc tcgagatcca gctcatcctc tccggcgaga aagacctcaa
      181 caatgccatc attacgattc accccggcgc gggcggcacg gaatctcagg actgggccca
      241 gatgctaatg cggatgtacc ttcgatgggc ggagaaggaa ggcttcaaga cggaacttct
      301 ggatctacag gccggcgaag aggccgggat caaggacgcg accttctcgg tcatcggcga
      361 atacgcctat ggaaagctca aggccgaagc gggggttcac cgccttgtgc ggatctctcc
      421 gtttgatgcc aacaagcgtc gtcatacctc gttcgcctcg gtttttgtat atcccgagat
      481 cgaggacgac gccgagctcg agctggacga gaaggagatc aagatggagg cctttcgttc
      541 cagcggtccg ggcgggcaaa acgtcaacaa ggtttcttcg gccgtccggc tcacgcacat
      601 ccctaccggt atcatcgtgg cctgccagac cgagcgatcg cagcacaaga atagatcggt
      661 ggcaatgaag ctgctgcgag ccaagctgta tgaggtggag aaagaaaaaa aagaagccga
      721 gatgaaccgt ctcgtgtcag cctccggcgg agcgaagaaa gaaatcacct ggggaagtca
      781 gatccgatcc tatgtctttc agccttacca aatggtcaag gaccaccgaa ccaatctcga
      841 gatcggaaac gtcaacgccg taatggacgg tgagattgat cccttcatcc aagcattcct
      901 gctgcaatct attaaatcaa atcgctaatc gcccggatca tttcttcacc ggctcttgag
      961 atctttcaag aactggcgta ttttttcctt cacctgtttc tctacctgct cctggacctt
     1021 gcctccgatc gcctgggtat ccaggacgta gaccg
//
LOCUS       DATMKH010000596         2459 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4447365, whole genome shotgun sequence.
ACCESSION   DATMKH010000596 DATMKH010000000
VERSION     DATMKH010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4447365"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1598
                     /locus_tag="VNV63_07100"
     CDS             <1..1598
                     /locus_tag="VNV63_07100"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HXC62425.1"
                     /translation="LRGQNLVGYRHYADDVVEKFVERSVANGINIIRIFDALNDLRNL
                     KTAIKATLKNGAKVEGTLCYTTSPVHSNELFVELAKRLEDMGSDTICIKDMAGLLAPL
                     TAYDFISKIKRAVSVPVHLHCHDTSGMALATCLKAVEAGVDIVDAAISPMASGTSQPP
                     TETFVAVLQASRFDTGLDLQMLAEIAGYFRETRKKYQAFETDYTGVDPAVLLYQIPGG
                     MTSNLASQLKEQKALGKMKQVLEEVIRVREEFGYPPLVTPTSQIVGTQATLNIITGER
                     YKVITSETKNYLKGLYGKPPAPINEEIRRKAIGDEEFNERRPADLLEPEMSKLKKELG
                     EKARSVEDVLSYALFPKVALEFFELREKGEMGKPEPVLTTKSAEEPPTAPAPLMAPSE
                     FNIKVHGETYHIKVGGMGHHGEGGRPYFIYVDDQLEEVLVESLVEVVPGAEGRIGVEM
                     TGHSTRPKATREGDITTAMPGTVVKVKVRIGDRVKAGETVLIIEAMKLENEVHTPIAG
                     EVKAIHVLEGDRVNPDEVLVVVR"
     gene            1595..2329
                     /locus_tag="VNV63_07105"
     CDS             1595..2329
                     /locus_tag="VNV63_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018289315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HXC62426.1"
                     /translation="MTDINQLIQTISIIVLPLLFAITLHEMAHGWIADKLGDPTARLA
                     GRLTLNPLAHIDPFGTVLLPLILMIPMLMGYPSMLFGYAKPVPVNYMNLRRPKQDMTW
                     VAAAGPGMNLVLAMLSGLLFRLLVILDPTLLLHVSRFSGFLHSGDLTSSILVPIVLMA
                     VMSVNINVLLMIFNLIPIPPLDGGRVLVGVLPNQQAIAVSRIEPYGMLIVIALLIFDP
                     QLGIMRTLVWPSVSIISNLILGAHIL"
     gene            2339..>2459
                     /locus_tag="VNV63_07110"
     CDS             2339..>2459
                     /locus_tag="VNV63_07110"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014321773.1"
                     /note="catalyzes a two-step reaction, first charging a
                     tryptophan molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HXC62427.1"
                     /translation="MKQRVLSGMQPSGRMHLGNLFGALNNWKKLQNEYDAYFFI"
BASE COUNT          574 a          620 c          701 g          564 t
ORIGIN      
        1 tgcttcgcgg ccagaatctt gtcggctatc ggcattatgc ggatgatgtc gttgaaaagt
       61 ttgtggagcg atcggttgca aacggcatca acatcatccg gatctttgat gccttaaatg
      121 accttcgcaa tctcaagacc gcaatcaaag cgacgctaaa aaacggtgcc aaagtcgaag
      181 gaacgctctg ctacaccacc agcccggtgc acagcaatga attgtttgtt gaactggcaa
      241 aacgtttgga agacatgggc tcggacacaa tctgtatcaa agatatggcc ggattattgg
      301 ccccgctcac ggcctatgat tttatctcca aaatcaagcg ggcggtgagc gtgccggtgc
      361 atctccactg tcatgacact agcggcatgg cactggccac ctgcctcaag gcggtggagg
      421 cgggcgtgga tatcgtggat gccgccattt ccccgatggc ttcgggtact tctcagccgc
      481 cgactgagac gttcgttgcg gtacttcagg catcgcgttt tgatacgggt ctcgatcttc
      541 agatgcttgc cgaaatcgcg ggttatttcc gagagacacg aaagaaatat caagcctttg
      601 aaacggatta caccggcgtg gaccctgctg tgctgctgta ccagattccg ggcgggatga
      661 cttccaacct cgcttcgcaa ttgaaagagc agaaggccct gggcaagatg aagcaggtgc
      721 tggaagaggt gatccgtgtg cgcgaggaat ttggctatcc gcctctcgtt acgccgacga
      781 gccagatcgt tgggacccag gccacgttga acatcatcac cggagagcga tataaggtga
      841 tcacaagcga aacgaagaat tatttgaaag gcctttacgg taaaccccct gcaccgatca
      901 atgaagagat ccggcggaag gcgatcggcg atgaggaatt caatgaacgc aggccggccg
      961 atctcctcga acccgagatg tccaagctaa aaaaggagct aggcgaaaaa gcacggtctg
     1021 ttgaagatgt attgagttac gctttattcc ctaaagtagc ccttgagttc tttgagctgc
     1081 gcgagaaggg agagatgggc aaaccggagc cggttttaac aacaaagtct gcggaggaac
     1141 cgccaacagc ccctgcgcct ctcatggcgc ccagcgagtt caacataaag gttcatggag
     1201 agacctatca tattaaagtc ggcgggatgg gccaccacgg agaaggagga cggccctact
     1261 ttatttatgt ggacgatcag ctcgaagagg tgctggtgga atccctcgtg gaagtagtcc
     1321 cgggtgcgga aggccggatc ggggtggaga tgaccggtca ttcgactcgg cccaaagcga
     1381 cacgggaagg ggacatcacc accgcgatgc cgggaaccgt ggtcaaggtc aaggtgagga
     1441 tcggggaccg ggtcaaggcg ggggagacgg tcttgatcat agaagcgatg aagctcgaaa
     1501 atgaagtgca cacgccgatc gcaggtgagg tcaaggcgat ccacgtcctg gaaggggatc
     1561 gtgtgaaccc ggatgaggtc ctcgtggtgg tgagatgacg gacatcaacc agttgatcca
     1621 aaccatctca attatcgtat tgccgcttct ctttgcaatt acccttcatg aaatggctca
     1681 tggctggatt gcggataaac tcggcgatcc tacggcgagg cttgccggcc ggcttacttt
     1741 gaatcctctg gcccatatcg acccgttcgg gaccgtcctt ctgccgctga ttcttatgat
     1801 tccgatgctg atgggctatc cttcaatgtt gttcggatat gccaaaccgg ttcccgtgaa
     1861 ctatatgaat ctccgacggc ccaagcagga catgacctgg gttgccgctg cgggtccggg
     1921 aatgaacctg gtgttggcaa tgctcagcgg cctgctcttt cgtctcctgg tcatactgga
     1981 tccgacgctg cttctccatg tcagccgatt cagcggtttt ttgcacagcg gcgatctaac
     2041 cagttccatc ctggtgccga ttgtattaat ggcggtcatg agtgtgaata ttaatgttct
     2101 cttgatgatc ttcaatctga ttcctatccc gccgctggat ggcggaagag tcctggtcgg
     2161 agttctgccg aatcagcagg caatcgccgt gagccggatc gagccctatg ggatgctgat
     2221 cgtcattgcg ctcctcattt ttgatcctca gcttgggatc atgcgaacgc ttgtctggcc
     2281 ttccgtcagc atcatcagca atctgatttt aggtgctcat atcctctagg agtagtggat
     2341 gaagcaacgc gttttaagcg gtatgcaacc gagcggccga atgcatcttg gcaatctctt
     2401 cggggcgctg aacaactgga agaaactcca gaacgagtac gacgcctatt tttttatcg
//
LOCUS       DATMKH010000597         1114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1563219, whole genome shotgun sequence.
ACCESSION   DATMKH010000597 DATMKH010000000
VERSION     DATMKH010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1563219"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..859)
                     /locus_tag="VNV63_07115"
     CDS             complement(<1..859)
                     /locus_tag="VNV63_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HXC62428.1"
                     /translation="MKADIVRRYSHNPILTKKDVPYPVETVHNAAVVKHKNEYIMLFR
                     SHLRTGRSILGLARSRDGFQFTADPHPFLTPAPDGPFAAYEEFGVEDPRVTLLEGEYV
                     ITYSAYSRNGVRIALAKTKDFSKVERVSLITEADYRNVVIFPEKFDGLYARLDRPHSE
                     ISPWSIWISYSPDLYFWGQSQLIMKPVPYHWDEMKIGPGAPPIKTEQGWLSIYHGVFK
                     TMDGSVYRLGVALHDLQNPAKIIGVGDSWILQPEDPWEVTGYVHNVVFTCGAIAEPDG
                     SVKIYWGGAD"
     gene            complement(859..>1114)
                     /locus_tag="VNV63_07120"
     CDS             complement(859..>1114)
                     /locus_tag="VNV63_07120"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXC62429.1"
                     /translation="ANAAGVPVIAMDLGSCREVIQDGQTGFLVNNVNEAVLVLERLSG
                     IDRRACRQRVQQYFSIETMVEGYEQVYATILDLREKEKRQ"
BASE COUNT          260 a          303 c          294 g          257 t
ORIGIN      
        1 tatccgcacc gccccaataa atcttcacgc ttccatccgg ctccgcgatc gcgccgcaag
       61 taaagaccac attgtggaca tagccggtga cttcccaggg gtcctccggt tgcagaatcc
      121 acgagtcgcc cacaccgata atcttggccg ggttctgaag gtcgtgaaga gcaacgccca
      181 aacgatagac cgatccatcc atcgtcttga aaactccgtg atagatggaa agccaaccct
      241 gttccgtttt gatcggcggc gcacccggac cgatcttcat ttcatcccag tgatacggta
      301 ccggcttcat gatgagctga gattggcccc agaaatataa gtcaggagaa taggaaatcc
      361 agattgacca gggagagatt tcagaatgag gacggtccag ccgggcatac agtccatcaa
      421 acttttcggg aaaaattaca acattccgat aatccgcctc ggtgatgagg gatacccttt
      481 ccaccttact gaagtccttg gtcttcgcta gggcaatccg cactccgttg cgcgagtagg
      541 cgctgtaggt gatgacgtat tccccttcca gtagagtcac ccgtgggtct tcaacaccga
      601 actcttcata ggcggcaaat ggaccgtccg gcgcaggagt gagaaagggg tgtggatcgg
      661 cggtgaattg gaatccgtcg cggctgcggg ccaaaccaag gatcgaccgg ccggtgcgca
      721 aatgggaacg gaaaagcatg atgtattcgt tcttatgttt gacgactgct gcgttgtgga
      781 ctgtctccac tgggtagggg acatccttct tggtgaggat ggggttgtgg ctatagcgtc
      841 taacaatgtc cgccttcatc actgcctctt ttctttttct ctcaaatcaa gaatcgttgc
      901 gtaaacctgt tcgtacccct ccaccatagt ttcgatagaa aagtattgct ggactcgttg
      961 acggcaagcg cggcgatcga ttccagaaag gcgttccagc actagcaccg cttcattgac
     1021 gttgttgacc agaaagccgg tctgtccgtc ctggatcacc tcacggcatg agcccagatc
     1081 catggcaatt acaggcacgc cggcagcgtt ggct
//
LOCUS       DATMKH010000598         1525 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4116533, whole genome shotgun sequence.
ACCESSION   DATMKH010000598 DATMKH010000000
VERSION     DATMKH010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4116533"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..132)
                     /locus_tag="VNV63_07125"
                     /pseudo
     CDS             complement(1..132)
                     /locus_tag="VNV63_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011318960.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
     gene            complement(313..555)
                     /locus_tag="VNV63_07130"
     CDS             complement(313..555)
                     /locus_tag="VNV63_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62430.1"
                     /translation="MLGALAYTFARIGAVVNLKVQDYYPSGKRFFLRFKEKGGKEKEL
                     PVHHKLEELLDEYLKAAASTASGKNEANNAGAIRLA"
     gene            732..>1525
                     /locus_tag="VNV63_07135"
     CDS             732..>1525
                     /locus_tag="VNV63_07135"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HXC62431.1"
                     /translation="MNTKPLALWVQAIVSRAAGYSTKSLCLIFALFSAAQAFAWTDGE
                     LLIWISSNRPFHALSNLGNAFEKEVGAPVKVETQEQIIDSFQSAAQSGKGPDIFFWAN
                     DRIGEWADSGLLKPLDIKEKFKAKYLPMSWDAVTHKQHIWGYPVALEAASLIYNKKLV
                     TGKVPTQLSELPAFGKELKAQNPNAIVIMWDYRTPYFSWPFLASAGGYPFKKTAEGYD
                     VNDIGVANAGALEGLKAIVELINTGILPKGSTLSVIGEKMASGQLAL"
BASE COUNT          379 a          400 c          411 g          335 t
ORIGIN      
        1 catcacggcc aggatgatca ctgcgccctt gataactaac tgccaataaa aggagacgtt
       61 catcaacgta agcccgttat taaggacggc gctgatcaga gctccgatca gcgtcccaaa
      121 gaccgttccg atccgggtga gaacggatca actcctccaa cagttggctg tcgaccgggt
      181 catctttggc gcaactggga taaaacgtct cgccgatagc gagctaaaga ttgaggattg
      241 acagggaaga tgtcgatcca atccgaatgc tgactcaggc tgtgaaagag cggaccacgg
      301 cgcgtcgacg gctcaagcaa gccggattgc ccccgcatta ttcgcctcat tctttccgct
      361 cgctgtcgag gccgctgctt tcaggtattc gtcgaggagt tcttcgagct tatggtggac
      421 gggcagctct ttctcctttc cgcctttctc tttgaatcgg agaaaaaatc gtttcccaga
      481 cggataatag tcttggacct tcagattcac gacggcaccg atccgggcga aggtgtaagc
      541 aagagcgccg agcaaagcgc gatcgcggag gccagtgtgt cctccgatcg cgccacttca
      601 ggagacctgc cgaaagagcg cgtttgttga gacggtgggt gtacccttgc attctggcag
      661 gaacaaagtc gtttcctacc tccgtcaaaa aatgatccaa tggatggatt gttggtaaac
      721 gcgtttccct tatgaataca aaacccctcg ctctctgggt acaggcgata gtttcccgcg
      781 cggcaggata cagtacaaag tctctctgtc ttatcttcgc acttttctct gcggcgcagg
      841 catttgcctg gaccgatgga gaactcctga tctggataag tagtaatcga ccttttcatg
      901 ccctgagcaa cctcggcaac gccttcgaaa aagaggtcgg agctccggtc aaagtagaga
      961 cccaagagca aatcatcgat agttttcaat ctgcggctca gagtggcaaa ggccccgata
     1021 tattcttctg ggcgaatgat cggatcggag aatgggccga ttcaggtttg ctaaagccgc
     1081 tggatatcaa agaaaaattc aaggcgaagt acctaccgat gtcgtgggat gccgtgacac
     1141 acaaacagca catttgggga tatccggttg cccttgaggc ggcgtcgctc atctacaata
     1201 agaagctcgt cacgggaaaa gtgcctactc agctttcgga actgcctgca tttggcaagg
     1261 aacttaaggc tcaaaacccc aatgcgatcg tgatcatgtg ggactacagg acgccctact
     1321 tcagctggcc gttcctcgcc agtgccggag gatacccgtt caagaagacg gccgagggct
     1381 atgacgtcaa cgacatcgga gtggccaacg caggagccct cgaggggctg aaggcaatcg
     1441 tggaactcat caatacgggc atcttgccga aaggatcaac gctaagcgta ataggtgaaa
     1501 aaatggcctc cggtcaattg gcgct
//
LOCUS       DATMKH010000599         2420 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4447717, whole genome shotgun sequence.
ACCESSION   DATMKH010000599 DATMKH010000000
VERSION     DATMKH010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4447717"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..1263)
                     /locus_tag="VNV63_07140"
     CDS             complement(58..1263)
                     /locus_tag="VNV63_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HXC62432.1"
                     /translation="MATFAWTGRTRQGVVQKGEASAKTRDEVISLLRKQNILVTSIRP
                     KSSMSLGGFMQAKVKEKDLAVFTRQFATMIDAGLPLVQCLEILTAQTENPTLAKVIGE
                     VRQEVEGGSTYADALRKHPKAFDELYVNMVAAGEAGGILDAILNRLAKHIEKAMKLKK
                     QIKSAMVYPGVIVTVATGVIVILMVWVIPIFAKLFSDFGSALPGPTQIVISSSYFMQS
                     YWYVILAIVGGIIYCLRRAYKTTAGRRLLDRFALSLPVFGDLIRKASVAKFTRTLGTL
                     IGSGVPILEGLIIVAKTSGNKIIEEALLKARQSISEGKTVSEPLGQAKVFPPMVVQMI
                     AVGETTGALDAMLGKIADFYDEEVDSAVGALTALLEPMLMVFLGVVIGFIVIAMYLPI
                     FKMAGAVSH"
     gene            complement(1275..2390)
                     /locus_tag="VNV63_07145"
     CDS             complement(1275..2390)
                     /locus_tag="VNV63_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015351907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HXC62433.1"
                     /translation="MANFYQLLQTMIEKGASDLHITTGTSPQIRVDGELIPLNLPALT
                     PAETKQLVYSVLTDAQKHRFEEDNELDLSFGLKGLSRFRANVFMQRGAVAAAIRTIPF
                     KIMSFEELGLPAVVNELVKRPRGLVLVTGPTGSGKSTTLASMIDKINTERHGHIVTVE
                     DPIEYLHTHKKCVVNQREVGADTNAFKTALKYILRQDPDVVLIGEMRDLETIEATLTI
                     AETGHLSFATLHTNSCAQTINRIIDVFPSHQQPQVRAQLSFVLEGVISQQLLPKAGGQ
                     GRVLALEVMVPNAAIRNLIREDKVHQIYSAMQTGQTKYAMQTMNQSLADLYQRRLITY
                     DEAVGRSSVPDELISMIGRSGAGAPAPAAREQRKPVG"
BASE COUNT          576 a          661 c          605 g          578 t
ORIGIN      
        1 tgagtttttg agcgagccct cgtccctgtg ctgcggatga atccatgccg gatatcttca
       61 gtggctcacc gcgccggcca tcttgaagat gggcaggtac attgcgatga cgataaaacc
      121 gatgacaacg cctaaaaaga ccatcagcat cggctccaac agagccgtga gggctcctac
      181 ggccgaatcc acttcctcat cataaaaatc agcgatcttt cccagcatcg cgtccaaagc
      241 accggtcgtt tcacccaccg cgatcatctg gaccaccatc ggaggaaaga ccttagcctg
      301 acctaggggc tccgaaacgg ttttcccttc actaatgctc tgacgcgcct ttagaagcgc
      361 ttcttctatg atcttatttc ccgatgtttt ggccacaatg ataagaccct ccaggatcgg
      421 gacaccgctg ccgatcaggg ttccgagagt gcgggtaaat tttgcaacgg aggccttacg
      481 aatcaagtca ccaaaaacag gaagcgaaag cgcgaaccga tccagaaggc ggcgaccggc
      541 cgttgtctta tatgcccttc ggaggcagta gatgatcccc cccacaattg caagaatcac
      601 ataccaataa gattgcataa aataactgga agagatcacg atttgtgtcg ggccggggag
      661 agcacttcca aaatcagaga acagcttggc aaaaataggg atgacccaaa ccatcaagat
      721 cacgattaca ccagtcgcaa ccgttacaat gacaccggga tagaccatgg ctgacttaat
      781 ctgtttcttg agcttcatcg ccttctcgat atgtttcgcc aaccggttta agatcgcatc
      841 cagaattccc cccgcttcac ccgccgccac catgttgaca tacagttcgt caaaggcctt
      901 ggggtgtttt cttaaggcat cggcataggt ggagccgcct tccacttcct ggcggacctc
      961 gccgattact ttggccagtg tagggttttc cgtctgagct gtcaggatct ccaagcactg
     1021 gacaagcgga agtccagcgt caatcattgt ggcaaattga cgtgtgaata cagccaggtc
     1081 cttctctttg accttggctt gcataaagcc gcctaaagac atgctgcttt tcggccgtat
     1141 cgaggtgacc aggatatttt gtttacgaag aagcgagatc acctcatcgc gggttttggc
     1201 acttgcctca cccttttgaa ccacaccctg tcttgttcga cccgtccatg caaaagttgc
     1261 cactgactat actcctatcc tactggtttg cgctgctccc gggctgccgg agccggagca
     1321 cccgccccac tcctcccgat catggagatc agctcgtccg gaacactaga acgcccgacc
     1381 gcttcatcat aagtaatcag gcggcgctgg taaagatccg ctaaagactg gttcatggtc
     1441 tgcatcgcgt atttggtctg tccggtctgc atcgcggagt agatctggtg gaccttatct
     1501 tcacgaatca ggtttcggat ggcagcgttt ggaaccatga cctccaaagc caggacacgg
     1561 ccttgaccgc cggccttcgg aagcagctgc tgtgagatta ccccctccag gacaaatgaa
     1621 agttgggctc tgacctgagg ctgttgatgt gatggaaaca catcgatgat tcggttgatc
     1681 gtctgggcgc aagaattagt gtgaagcgtt gcaaagctta agtgccctgt ctcagcaatc
     1741 gttaaagtgg cctcgatcgt ttcaagatct ctcatttccc cgatcaaaac gacatcagga
     1801 tcctgccgaa ggatgtactt taaggcagtc ttgaaggcat tggtgtccgc acccacctct
     1861 cgttgattaa cgacgcactt tttgtgggtg tgaagatact cgatcgggtc ttcgacggtc
     1921 acaatgtgac catgccgctc cgtattgatc ttgtcgatca tggatgccag agttgtggac
     1981 tttccgctgc cggtgggtcc ggtcaccaag accaaaccgc gggggcgttt gaccagctca
     2041 ttgacgacag ccggaaggcc cagctcttca aaactcataa tcttaaacgg gatggtccga
     2101 atagcagctg ccacagcccc gcgttgcatg aagacattgg cccggaaacg gctcaggccc
     2161 tttaatccaa aagagaggtc gagctcgtta tcttcttcaa accgatgctt ttgggcatcc
     2221 gtcaggacgc tatagacaag ctgcttggtc tcggctggag tgagtgccgg aagatttaaa
     2281 ggaataagct ccccgtctac acggatctgg ggagacgtac cggtagtgat atgaagatcc
     2341 gacgcccctt tctcaatcat cgtctgaagt agttgataga agttggccat gtttgccctt
     2401 ttatctaatc gctaaaggtg
//
LOCUS       DATMKH010000600         3331 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1871205, whole genome shotgun sequence.
ACCESSION   DATMKH010000600 DATMKH010000000
VERSION     DATMKH010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3331
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1871205"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="VNV63_07150"
     CDS             complement(<1..544)
                     /locus_tag="VNV63_07150"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012031678.1"
                     /note="catalyzes the formation of L-citrulline from
                     carbamoyl phosphate and L-ornithine in arginine
                     biosynthesis and degradation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HXC62434.1"
                     /translation="MKKSRHLLAVHDLTQAEILNLFKRAKALKKRKARVLVGRTLGLF
                     FEKSSTRTRVSFEVAMAQLGGHSIFLSKEESQIYRGETISDTARVLSRYLDGLVIRTY
                     DHHTIEEWARHATIPVINGLTDLHHPCQALGDLFTVFEKRGRISGLKLAYIGDGNNVA
                     NSLIEGALRLGMNISLACPKG"
     gene            complement(541..1743)
                     /locus_tag="VNV63_07155"
     CDS             complement(541..1743)
                     /locus_tag="VNV63_07155"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018154736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HXC62435.1"
                     /translation="MSQEELIEESKKYLMNTYARQPVVFRKGRGNRLYDVTGKEYIDF
                     VSGIAVNNLGHCSPRVTVAFQKQAQRLVHVSNLYHNEPQIHLAKLLVEHSFADKVFFC
                     NSGAEANEAAIKLVRKYARTQRGEDRFEIITMEQSFHGRTLGTLSATGQTKYQKDFEP
                     MLPGFQYVPFNDVGAVAKAITAKTIAVLVEPIQAEGGVRVPSDDYLSRLRKLCDEQGL
                     LLILDEVQTGMGRTGRLFAYEHYGIEPDIMTLAKGLGGGIPIGAMLAKASVADAFVPG
                     SHASTLGGNPLACAAAFATIEAMLEEDGFILDHCQLMADYFEKGLWSLAKRYPVIKEV
                     RGKGLLIGVELAIEGKPIVQACLEEGFLINCTMDRVLRFIPPLIITQEEIDLLLETLE
                     RIFKRGIG"
     gene            complement(1903..2502)
                     /gene="pyrE"
                     /locus_tag="VNV63_07160"
     CDS             complement(1903..2502)
                     /gene="pyrE"
                     /locus_tag="VNV63_07160"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007355286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HXC62436.1"
                     /translation="MTALPKRKSADEIKTLKTRLLKLLYKKSFQQNPDPIFRLSSGRM
                     SPYYINAKKTTLDPEGAYLIGKIIFRLIRGLQADGIGGLTLGADPIAGAVALTSFIEK
                     KPIPAFIIRKEPKGHGTQAWLEGSLPSGARVVVLEDVVTTAASALKAIEKLKEAGHTV
                     IKVIALVDRQEGGEQNLAEAGYTLDALFNLDDLKTLSAS"
     gene            2856..>3331
                     /locus_tag="VNV63_07165"
     CDS             2856..>3331
                     /locus_tag="VNV63_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HXC62437.1"
                     /translation="MKNNAMESEPTRPSRPMLVKDHEQQLSLMIASVKDYAIFMLNPE
                     GRIMSWNTGAEKIKGYTENEIIGKHFSIFYPSEEIQAGKPERELKTALAEGRFEEEGW
                     RLRKDGSLFWANVVITPVYNGAEALLGFSKVTRDLTERKYAEETLRQAQEQFIQASR"
BASE COUNT          787 a          868 c          873 g          803 t
ORIGIN      
        1 atccctttgg acaagccagc gagatgttca tcccaagccg cagtgccccc tcgatgagcg
       61 agttggcgac attgtttccg tcgccgatgt aggccagctt caggccggag atgcggcctc
      121 ttttttcgaa gaccgtgaaa aggtctccca gcgcttggca ggggtgatgg agatcggtca
      181 ggccattaat caccgggatc gtcgcgtgcc gtgcccattc ctcaattgtg tgatggtcgt
      241 acgtccggat cacgagccca tcgaggtaac gggacagtac acgagcggta tccgagatcg
      301 tctctccccg ataaatctgg ctctcttctt tcgacaagaa gatcgaatgg ccgccgagct
      361 gggccatggc cacctcaaac gaaacgcgag tccgcgtgga ggacttctcg aaaaaaagcc
      421 ccagtgtacg gcccaccagt acacgggcct tccgcttctt gagcgccttc gcccttttga
      481 acagattcaa gatctccgcc tgcgtgagat catggaccgc taggaggtgt cttgactttt
      541 tcatccgatc cctcgcttga agatgcgctc cagtgtctcc agaaggagat caatctcttc
      601 ctgtgtgatg atcaacggtg gaatgaaccg aagcacacga tccatcgtgc agttgatcag
      661 aaatccttct tccaggcagg cctgaacgat cggctttcct tcgatggcca gctcaactcc
      721 gatgagaagg ccttttcctc tcacctcctt gatcacagga taacgtttcg ccagagacca
      781 gagacctttt tcaaaatagt ctgccatcag ctggcaatga tctaggatga agccatcctc
      841 ttccagcatc gcctcgatcg ttgcgaatgc agcggcgcag gccaatggat tgccgcccaa
      901 ggttgaggcg tggcttccgg gtacaaatgc atccgccacc gaagccttgg ccagcatcgc
      961 tccgatcggg ataccgccgc ccaagccttt ggccagcgtc atgatgtccg gctcgatccc
     1021 ataatgctca tacgcaaaca gccggcccgt ccgtcccata ccggtctgga cctcatccag
     1081 gatcaggagc aacccctgct catcacacag tttccggagc cgggagagat agtcatccga
     1141 gggtacacgg acccctccct ccgcctgaat cggctcgacg agaaccgcga tcgttttggc
     1201 tgtaatcgcc ttggcgaccg cgccgacgtc gttaaaaggg acgtactgga aaccgggaag
     1261 catcggctca aaatcttttt ggtacttggt ctggccggtg gccgagagtg ttccgagtgt
     1321 acggccgtgg aaggactgtt ccatcgtgat gatttcaaat cgatcttcgc ctcgttgggt
     1381 ccgggcgtat ttacggacca gcttaatcgc ggcctcgttt gcctcggcac cgctgttaca
     1441 gaagaagacc ttatccgcaa aggagtgctc aaccaggagc ttggcgagat ggatctgagg
     1501 ctcgttgtgg tacaagtttg agacatggac aagccgctgg gcctgcttct ggaaggccac
     1561 ggtcactctg ggcgagcagt ggccgaggtt attgaccgca attccgctta cgaaatcaat
     1621 gtactccttg ccggtgacat cgtagagacg gtttccccgt cccttccgaa acaccacggg
     1681 ctgccgggcg tacgtgttca tcaaatattt tttcgattct tcgatcagct cttcttgaga
     1741 catgttttgt tcagtagaca tgtagaaacg gcttattggg aagataccta aataccacat
     1801 tctacataga aaagcaatca aaaactttgc cggaaaaatc ggggtagtgg tcggttttga
     1861 agtagctagt taggagtaaa tcataaaaaa actggccgct gactaagaag ctgaaagggt
     1921 ctttagatca tccaaattga aaagggcgtc cagagtgtag cccgcttccg ccaggttttg
     1981 ctcgccgccc tcctggcgat ctaccaacgc aatgactttg atgacggtgt gccccgcctc
     2041 tttgagtttc tcaattgcct tcagggcgga tgcggccgtg gtgacgacat cctcaaggac
     2101 cacgacccgc gccccagacg gcaggctgcc ttccagccag gcctgagtcc cgtggccctt
     2161 gggttccttc cggatgatga atgccggaat cggttttttc tcgataaaac tggttagagc
     2221 aacagccccg gcgatcgggt cggctcccaa cgtcaaaccg cctattccgt ccgcttgaag
     2281 tccacggatg agccgaaaga tgatttttcc gatcagatag gcgccctccg gatcgagcgt
     2341 cgttttcttg gcattaatgt aatacggact catacggccg gacgaaagac ggaaaatcgg
     2401 atcagggttt tgctggaagg attttttgta gagcagtttt agaagtcttg tcttgagggt
     2461 ttttatctcg tcagccgatt ttcgtttggg tagggcggtc attaagagga gctttgttaa
     2521 aaggagtttt gttgcaaatg aatcagattt aggataacat gcacgagctt ataattattg
     2581 tcaagggcat gagagtcgca agcgatgaac cccatattaa gaattcttct tttcatctgc
     2641 ggtgccttag ccgtgttgac catgattttc tacctactgg gtatatacag tctcgggccg
     2701 atggaataac gatttcaccg cttgacctta ggcggtatcg taacagaggc tctttttaga
     2761 atcaagtccc aaaatacagg atttacctga ttttcattta tgttttcgta gtctaaaatt
     2821 tagctgatac aactctcttt aaatgaggta actccatgaa aaataatgcg atggaatcag
     2881 aaccaactcg cccatcgcgg ccaatgttgg ttaaggacca cgaacagcag ttaagcctaa
     2941 tgatcgccag cgtgaaggac tatgcgattt tcatgctcaa tccggaaggc cgcattatga
     3001 gctggaacac aggcgcggaa aagatcaagg ggtatacaga aaatgaaatt atcggcaagc
     3061 atttctcaat tttttatcct tctgaagaga ttcaagctgg caaacccgag cgcgagctca
     3121 aaacagccct cgccgagggg cggtttgaag aggagggctg gcgcctgcgt aaggacggtt
     3181 cgctgttttg ggcaaatgtg gttatcactc cggtttataa cggggcagaa gccttgctcg
     3241 gattctcaaa agtgacgcgc gacctgaccg aacgcaagta tgcagaggaa acactgcggc
     3301 aagctcaaga acagttcatt caggccagcc g
//
LOCUS       DATMKH010000601         1007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2847717, whole genome shotgun sequence.
ACCESSION   DATMKH010000601 DATMKH010000000
VERSION     DATMKH010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2847717"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VNV63_07170"
     CDS             complement(<1..294)
                     /locus_tag="VNV63_07170"
                     /inference="COORDINATES: protein motif:HMM:NF014919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HXC62438.1"
                     /translation="MDNRLYSQGAEIVPGGVSFRTWADRRKQVSVAIFAEDGSILREL
                     SLSEEGDGYRSALDPASGVGALYKYRIDGNLFPDLASRFQPKGVHGPSQVVDGR"
     gene            complement(284..>1007)
                     /locus_tag="VNV63_07175"
     CDS             complement(284..>1007)
                     /locus_tag="VNV63_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glycogen debranching enzyme GlgX"
                     /protein_id="HXC62439.1"
                     /translation="NALRRRQQRNFLATLILSQGVPMINAGDEFSRTQQGNNNTYCQD
                     NPLNWFNWNWDDDAKALFDFTKRIIKFRKDHPVFRRPKYFMGRRIRGSDVKDIMWFNP
                     SGAEMNDQDWTNGFAKCLGMLVSGSTIDIRDFKGEPIHDDTFLLLFNAHYEPLTFILP
                     GREEIRWELLLDTSKERGFLKTHKVTSAGEEVELVDRSICLLKLSEGEESFAREESWT
                     TKAGKAPSKVPSKPAEPVTDGQ"
BASE COUNT          226 a          268 c          244 g          269 t
ORIGIN      
        1 ccttccatca actacttgcg atggaccatg gacacccttc ggttgaaaac gggaagcgag
       61 atccggaaag agattgccgt cgatccgata cttgtagagt gctcccacgc ccgacgccgg
      121 atcaagggca ctgcgatagc cgtcgccctc ctcggagagc gacagttcgc gaaggatgct
      181 tccatcctct gcgaaaatgg ccacagacac ttgtttccgt cggtccgccc aggtccggaa
      241 cgagactccc cctggcacta tttccgctcc ttgagaatat agtctattgt ccatcggtta
      301 ctggttcagc gggtttactg ggcaccttag agggagcttt cccggctttg gtcgtccagg
      361 attcttctcg agcaaagctc tcttcgcctt cggaaagctt caatagacag atgcttctgt
      421 cgaccaattc gacctcttcg cctgccgagg ttactttgtg ggttttgaga aaaccccgct
      481 ctttgcttgt gtcgagaagc aattcccatc ggatctcttc ccgtcctggc aagataaacg
      541 ttaacggttc gtaatgagca ttaaacaaca aaaggaaagt gtcatcgtga atcggttctc
      601 ccttgaaatc tcgaatatca atggttgatc cgctcaccag catcccgaga catttcgcga
      661 atccatttgt ccagtcctgg tcattcatct cagctccgga tggattgaac cacatgatgt
      721 ccttgacatc ggatccccga atccgccttc ccatgaaata tttgggccgc cgaaagaccg
      781 gatggtcctt tcgaaatttg atgatgcgtt ttgtaaaatc aaacagagct ttggcgtcgt
      841 cgtcccagtt ccaattgaac cagttcaatg gattatcctg gcagtaggta ttattgtttc
      901 cttgttgagt gcgcgaaaat tcgtctccgg cgtttatcat cgggactccc tgagagagaa
      961 ttaaagtggc gaggaagttg cgttgctggc gtcttcgaag cgcattt
//
LOCUS       DATMKH010000602         1154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4116974, whole genome shotgun sequence.
ACCESSION   DATMKH010000602 DATMKH010000000
VERSION     DATMKH010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4116974"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="VNV63_07180"
     CDS             complement(<1..447)
                     /locus_tag="VNV63_07180"
                     /inference="COORDINATES: protein motif:HMM:NF018516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
                     /protein_id="HXC62440.1"
                     /translation="MALPETILNPIPGENPSGQNLRYAPVYDKIKEARREEVDLPQGG
                     WSRELKLADSKVVIKLATEALSTKTKDLQLAAWLTEGLLREEDFGGLKEGLDLLHALV
                     STFWDTVYPEAEDGDVELKVAPLSWVGTYLGEQVRKAEITKSGFNFL"
     gene            complement(454..>1154)
                     /locus_tag="VNV63_07185"
     CDS             complement(454..>1154)
                     /locus_tag="VNV63_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system accessory protein TagJ"
                     /protein_id="HXC62441.1"
                     /translation="GKLNEAIGALNEQLRRDPTNGKARTFLFELLCFSGEYDRAEKQL
                     AVLSQGGQQSELGGLLYRGALSATRARQDLFEKKEFPKPVGAAENKGSVSGTINGTPF
                     QTMVDADSRVGANLEVFAAGSYFWLPFAFLSSIEISPPKRLRDLLWIPAIIRTSAEYQ
                     DKELGETLLPALTPFSGKHADDSVRLGRETLWEEDEDGQDYPIGQKMLLADDQEIPIL
                     EVRKLEFTSPPKSS"
BASE COUNT          247 a          331 c          299 g          277 t
ORIGIN      
        1 caaaaaattg aaaccactct tggttatttc cgcctttcgg acttgttccc caagataagt
       61 tcccacccaa ctcagcggcg ccactttcaa ttccacgtcg ccatcttcag cttccgggta
      121 gacggtatcc cagaaagtcg agaccagagc gtgcaacaga tcaagcccct ctttgagccc
      181 cccgaaatcc tcctctcgca ggagcccttc ggtaagccag gcagccaatt gcaggtcttt
      241 ggtcttggtg gaaagggcct cggtagccag tttgatcacc accttggaat cggccagctt
      301 taattcccgt gaccagcccc cttgcggaag gtctacttcc tcgcggcgtg cttccttgat
      361 tttgtcgtat acgggggcgt aacggaggtt ttgcccactt gggttttccc cgggaattgg
      421 attgaggatg gtctcaggga gcgccatgat tgcctaagaa gactttggtg gagacgtgaa
      481 ctccagcttg cgaacttcta gaattggtat ctcttggtca tccgccagca acattttctg
      541 tccgatcggg tagtcctggc catcctcgtc ttcttcccac aaagtttctc ttccaagcct
      601 caccgagtcg tccgcgtgtt ttccggagaa gggagtgagc gcggggagaa gagtctcacc
      661 gagttctttg tcttgatact cggcagaggt gcgaattatc gcgggaatcc agagcagatc
      721 gcggagccgc ttcggggggc tgatttcgat cgaggagagg aatgcaaacg ggagccagaa
      781 atagctgccg gcagcgaaaa cctcgaggtt ggcgccaacg cgcgaatccg catccaccat
      841 tgtctgaaag ggcgttccgt tgatggtgcc cgaaacagat cccttgtttt ccgccgcgcc
      901 aacgggcttg gggaattcct ttttctcaaa aagatcctgc cgcgcgcgag ttgcactcag
      961 agcaccccgg tatagcaagc cccctaattc tgattgctgt ccaccctgag aaagaacggc
     1021 cagttgcttt tccgctcgat catattcccc actgaaacaa agcaactcaa ataaaaaagt
     1081 acgagccttc ccgttcgtcg gatcgcgccg cagttgttcg ttcagagccc caatggcttc
     1141 attgagcttc ccgg
//
LOCUS       DATMKH010000603         1542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3789230, whole genome shotgun sequence.
ACCESSION   DATMKH010000603 DATMKH010000000
VERSION     DATMKH010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3789230"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /gene="nth"
                     /locus_tag="VNV63_07190"
     CDS             <1..613
                     /gene="nth"
                     /locus_tag="VNV63_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006056614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HXC62442.1"
                     /translation="SELKKLYPNTKYYLNFSSPLELLVATVLSAQVRDTVVNATTPRL
                     FQKYKTAEDYARADLETLIQEISSVSFPANKAKYIRQACKILVEKYHGKVPDTMEQLI
                     ELPGIGRKTANVILINAFKKVEGIVVDTHVIRLSYRLGWTKNKNPEKIEQDLMKIIPK
                     ADWDRITWLLKEHGRAICRAPVPICSQCALEPLCPKAGVTKRV"
     gene            complement(620..1396)
                     /locus_tag="VNV63_07195"
     CDS             complement(620..1396)
                     /locus_tag="VNV63_07195"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXC62443.1"
                     /translation="MNEALQEITAMTMRWFRRLSREPITLVFGLIQPMLFWLILFGNQ
                     FKNVARLPGFQGTDYINFLTGGVIVMTVLNSGLAGGVDLLFDKENGFLERLMAAPIHR
                     SSLILSRFLFVMVITGFQILVLLVIAYLFFGVYPVSGLGGIALILFIGLLFGLGLISL
                     SLALALRIKGHGAFFSLIGFFSTPLIFLSGALVPIQAMPLWMQLLARLNPMTYAIDAI
                     RGLILHGFLWRTFLAVTGVLILFDALCLVGGNLVFKRSLG"
BASE COUNT          425 a          402 c          406 g          309 t
ORIGIN      
        1 atcagagctc aaaaagctct acccaaatac caaatactat ttgaatttct ctagcccgct
       61 ggagctcctc gtggctacgg ttctttcagc ccaggtccgt gataccgtgg tcaatgcaac
      121 tacgccccgg ctgtttcaga aatataaaac ggctgaggat tatgcccggg cggaccttga
      181 gacgctgatt caggagatca gctcggtttc ttttccggcc aacaaggcca agtacatcag
      241 gcaggcatgc aaaatcctgg ttgagaaata tcatggaaag gttccagaca cgatggagca
      301 actgatcgaa ttgcccggca ttggtcgtaa gaccgccaat gtcatactga ttaatgcttt
      361 taaaaaggtc gagggtatcg tggtggacac ccatgtgatc cggctttctt accgtttggg
      421 ctggaccaag aataaaaatc cggaaaagat cgagcaggat ctgatgaaga ttatccccaa
      481 ggctgactgg gaccgaatta cctggttact caaggaacat ggaagggcga tttgcagagc
      541 gcccgtccct atatgcagtc aatgtgcgct ggaaccgctc tgtcccaaag cgggtgtcac
      601 gaagagagta taagcgaagt tacccgagac ttctcttgaa aacgaggttg ccgccgacca
      661 aacagagggc atcaaacagt atcagcactc cggtgaccgc caggaaagtt ctccataaga
      721 agccatggag gattaatcca cgtatcgcat caatcgcata ggtcattggg ttcaatctgg
      781 ccaacagctg catccaaagc ggcatggcct gaatcggaac cagcgcgccg cttaagaaaa
      841 tcagcggcgt tgagaaaaat ccgatgaggg agaagaaggc cccgtgccct ttgatccgta
      901 aggccaaggc aagcgagagc gagatcagcc ccaatccaaa caatagtccg atgaacagta
      961 ttaacgcgat tcccccgaga ccgctgaccg gatagacacc gaaaaacaga tacgcaatca
     1021 cgaggaggac aagaatttga aagccggtga tgaccatcac gaaaaggaac cgactcagta
     1081 tcagcgagct ccggtgaatc ggggccgcca tcaatcgttc taagaaccca ttctctttat
     1141 cgaacagcag atccacgccg cctgcgaggc cgctgttgag gacggtcatc acgattacgc
     1201 cgcccgtgag aaagttgatg tagtccgtgc cctgaaaacc tgggagcctg gcgacattct
     1261 tgaactggtt gccgaaaagg atcagccaga agagcatcgg ctgaatgagg ccaaagacga
     1321 gcgtgatcgg ttcccggctt aagcgccgga accagcgcat cgtcatggca gtaatttcct
     1381 ggagcgcttc attcatgagc ggtcccggtg agcgactctt cggaagggat ctcttccttg
     1441 atcccgtgac cggtgtaagt gacgaagaca tcctccagac tcggacgact gtactgaacc
     1501 gtctcaacat ggcagcctgc atcagcgaca cgcttcagga cg
//
LOCUS       DATMKH010000604         1622 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4450062, whole genome shotgun sequence.
ACCESSION   DATMKH010000604 DATMKH010000000
VERSION     DATMKH010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4450062"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..103)
                     /locus_tag="VNV63_07200"
     CDS             complement(<1..103)
                     /locus_tag="VNV63_07200"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXC62444.1"
                     /translation="MTERVAVITGGAKGIGRAIGMALAQDGWSIALCY"
     gene            complement(111..950)
                     /locus_tag="VNV63_07205"
     CDS             complement(111..950)
                     /locus_tag="VNV63_07205"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXC62445.1"
                     /translation="MELEDEFCDIIGKARWGKGLSLEDLADKTGLPADAITSLEKGSS
                     KPTEDQVKKLAQALSLELQRLLLIAKGQYLPKPTSTEILKHLIALSGDLGGYRVWGYL
                     LYDPESREAALFDTAYDPKLSLDAIAKNGLRLRFIFLTHCHYDHIGGSEEIRAATGAQ
                     MVCHQKESPLYRHANVKEPDLLIEDGNEIRLGRWNVRVVETPGHTPGGVTYLADGMAF
                     VGDALFAGSTGRSMSSSGYRMLISSLREKVLSLEPDTVLFPGHGPSTTVGEEREHNPF
                     FIM"
     gene            1091..>1622
                     /locus_tag="VNV63_07210"
     CDS             1091..>1622
                     /locus_tag="VNV63_07210"
                     /inference="COORDINATES: protein motif:HMM:NF019968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62446.1"
                     /translation="MAETQEIAWTPEAEKSIERAPVFLRGMVRKLAEKKARELGLTSI
                     TAEHLAAWKNQTMGAMGGQDSLQKAADQIKEGHLPWTQQAKERLETVPIFMRVMVKQI
                     ADEIALERGHLEVNVELLQKVESMGEVDDSAERPEMSWTEGARHRLEKKIEHAPAVAM
                     DFVSQMLERDAEDLARQ"
BASE COUNT          393 a          404 c          459 g          366 t
ORIGIN      
        1 gataacagag tgcgatagac cagccgtctt gagccagtgc catgccgata gcgcgcccga
       61 tgccctttgc gccacccgtg ataaccgcaa ctctttccgt catagatgcc tcacattata
      121 aaaaacgggt tgtgttctct ctcttcgcct acggttgtgg acgggccatg gccggggaat
      181 aaaaccgtat caggttctaa tgacagaact ttttcgcgta atgatgaaat gagcatccgg
      241 tacccggacg acgacatgga acgaccggtt gacccggcga acagggcatc acccacaaaa
      301 gccattccgt cggccaaata ggtgactccg ccaggtgtgt gtcccggagt ttccaccacc
      361 cggacattcc agcgccccaa ccgaatttca tttccgtctt cgatcagtag atccggttcc
      421 ttgacattgg cgtgccgata aagtggcgac tctttttgat ggcacaccat ctgtgctccg
      481 gtcgcggctc tgatctcctc gctgccgccg atgtgatcgt aatggcaatg ggttaagaaa
      541 ataaaccgaa gacggaggcc atttttggca atggcatcca gcgaaagttt cggatcgtag
      601 gctgtatcaa ataatgcagc ctcacgagat tcgggatcgt ataacaaata gccccaaacc
      661 cgatagcctc cgagatcgcc ggagagggca atgagatgtt ttaagatctc cgttgatgtc
      721 ggtttgggga gatactggcc ttttgcaatc aagagaagtc gttgtagctc aaggctcagc
      781 gcttgggcca gttttttgac ctgatcttct gtgggcttac tcgatccttt ttctagggat
      841 gtgatggcat cggcaggcag accagtttta tctgcaaggt cttcgagact tagtcccttg
      901 ccccaccggg cctttccgat aatatcgcaa aattcgtctt cgagttccat gaatccttct
      961 atttgttaga gaattgtttt tttgaggata tacctaaatg cgcggagaaa tcaataggcg
     1021 caagcggtta ggcggagttt gactcgcaaa tccaaactgt gctaccatgg gttccacttt
     1081 cgaggagaat atggccgaga ctcaagagat tgcctggaca ccggaggctg agaagagcat
     1141 agagagggca ccggtatttc tgcgcggcat ggtcagaaag ctggcggaga agaaagcgcg
     1201 cgagctgggg ctgacctcaa ttaccgcaga gcatcttgcc gcatggaaga accagaccat
     1261 gggtgctatg ggtggccagg actctctcca aaaagcggcg gatcagatca aggaaggcca
     1321 tctcccctgg actcagcagg caaaagaacg attggagacg gtgcccatat tcatgcgcgt
     1381 catggtgaag cagatcgccg acgagattgc cttggagcgg ggacatctcg aggtgaacgt
     1441 ggaacttctc cagaaggtgg aatccatggg cgaggtagat gattccgcag agcggccgga
     1501 gatgtcctgg acggaaggag cccggcatcg gcttgagaaa aagatcgagc atgcaccggc
     1561 ggtggccatg gattttgtgt ctcagatgct cgaacgggat gcggaagacc tggcccggca
     1621 ga
//
LOCUS       DATMKH010000605         1111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_283300,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000605 DATMKH010000000
VERSION     DATMKH010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1111
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283300"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..873)
                     /locus_tag="VNV63_07215"
     CDS             complement(<1..873)
                     /locus_tag="VNV63_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62447.1"
                     /translation="MTLDTHDSSTTATLDESQIDESQIRDEAALPDETQDYFETAVPD
                     EFPANPEILDEAARKLEKKQRKAQKKAEKKKLKHKLKAKKSSTKSKAVTFPMVELIDE
                     QVRTSIPGALVAKVKAAKSKKDIINSNYPYRKKMKESEYLEEIELLQIELVKMQAWVK
                     EVGERIVLIFEGRDAAGKGGTIQRFTENLNPRGAKIVALAKPSDTERGQWYFQRYIEN
                     LPTKGEIVFFDRSWYNRGGVEHVMGFCTPHEYLEFMRQTPEFERMMVRSGIRVFKFWF
                     SVSREEQLRRFLGRA"
     gene            complement(933..>1111)
                     /locus_tag="VNV63_07220"
     CDS             complement(933..>1111)
                     /locus_tag="VNV63_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HXC62448.1"
                     /translation="DRGASIRMPVNFIKNGYKGYLEDRRPNSEGDPYLIVSRVLKTIN
                     SVPKPELVRSTSSA"
BASE COUNT          221 a          303 c          285 g          302 t
ORIGIN      
        1 agcccggccg agaaaccggc gcaactgttc ctcccggctg acagaaaacc agaatttaaa
       61 aacccgaatc ccgctgcgca ccatcatccg ctcaaattcc ggggtctggc gcatgaattc
      121 caggtattcg tgcggggtgc aaaaccccat cacatgttcg acgcccccgc gattgtacca
      181 ggaccggtcg aagaagacga tttcaccttt agtgggaagg ttttcgatgt aacgctggaa
      241 gtaccactgg ccccgctccg tgtccgaagg tttggcgagg gccacgatct tggcgccgcg
      301 aggattgaga ttttcggtga accgctggat ggttcctccc ttgcccgcag cgtcgcgccc
      361 ttcaaagatc agcacgatgc gctcgccaac ttccttgacc caagcctgca tcttgaccag
      421 ttcaatttgc agcagctcga tctcttccag atactccgac tccttcatct ttttccgata
      481 aggatagttg gagttaatga tgtctttctt gctcttagcc gccttaacct tggcgaccag
      541 tgctccggga atgctggttc gcacctgttc gtcaatcagt tcgaccatag ggaaagttac
      601 ggccttcgat ttggtggagc ttttcttcgc ttttagttta tgtttaagtt tctttttctc
      661 agctttcttt tgggcttttc gttgtttctt ttctagcttt cgggcggctt catccaagat
      721 ctctggattc gccggaaatt cgtccggcac ggcagtctca aagtaatctt gcgtctcgtc
      781 ggggagggca gcttcatccc ttatctggga ttcgtctatc tgagattcat cgagtgtggc
      841 agtcgtagaa ctatcgtgag tgtctaacgt catgatgtcc tcccgggcac tgcagtatgc
      901 cctcccgggc actgcgctgt cagaaactga ctttatgcag aagaagtaga tctgacgagt
      961 tcaggcttcg ggacgctatt aatggtcttc agaacgcgtg agacgatcag gtagggatcg
     1021 ccttcggagt tcgggcggcg atcttccaga tagcccttgt acccgttctt gatgaagtta
     1081 accggcatcc ggatcgaagc gccacgatcc g
//
LOCUS       DATMKH010000606         1246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_601213,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000606 DATMKH010000000
VERSION     DATMKH010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_601213"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..>1246
                     /locus_tag="VNV63_07225"
     CDS             75..>1246
                     /locus_tag="VNV63_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62449.1"
                     /translation="MNEYRAMRMMNMADRMNPQARSHFSGGRGGLGQNPMLSSMLSGE
                     AQQPDYSSFTYDPAARQAAQQYLGQFGLSPVAPQNVQHNAFLPNTGFFGNHPRLSGML
                     EGGLFGAAATRGSDTWGEGISNVAGGMLEAKMARQGMINQQFAKPFQAAHMLEGMRDM
                     TQKRELQSMEIEHMRVENQKLGRPDHDMRSIPMTRNDVGVYNYDNTDGKGQFVKNPDY
                     DPKGNPIPHDPWDDYKNAHPEQSVDEQLKGYHKLIQAPEREAPVHVPFFSSDGTLRDL
                     RPGQKMPAGSVKVADWEKLNPQQKERITQIHNQLKVLNSPQTLKQRSMQRMFDPAWQQ
                     AHPNAKPGEAGKSVAADIQSEINALNKEAQDISGSAGGNPMGGGQGTATYNQATGQ"
BASE COUNT          338 a          335 c          312 g          261 t
ORIGIN      
        1 tttcaccaca atgatgatgc aataatatgg cagacaatcc ttttcaacaa ttcggtggcg
       61 agaatgccgg agccatgaac gagtatcgcg ccatgcgtat gatgaacatg gccgatagaa
      121 tgaatcccca ggcacgttct catttctctg gaggtcgagg aggcttgggg cagaatccta
      181 tgctctcttc gatgctctca ggcgaggccc agcagccaga ttattcttcc tttacctatg
      241 accctgccgc tcggcaagct gcccagcaat acctaggaca gtttggtttg tctccagtag
      301 ctcctcaaaa tgtccagcac aatgcctttc ttccgaacac cgggttcttc ggcaatcatc
      361 cccgcctgtc ggggatgctc gaaggaggct tgtttggtgc agccgccacc cgaggttcag
      421 acacctgggg cgagggaatc tccaatgtag ccggaggcat gcttgaggcc aagatggccc
      481 gccaagggat gatcaatcag caatttgcaa agcccttcca ggcggctcat atgcttgagg
      541 ggatgcgcga catgactcag aagcgggagc ttcaatccat ggagatcgag cacatgcgcg
      601 tagagaatca aaaactcggt cgcccggatc atgatatgcg atccatcccg atgacgcgca
      661 atgatgtcgg ggtttataat tatgacaaca cggatgggaa gggccagttt gtaaagaacc
      721 cggactatga tcctaaaggt aatcctattc cccatgatcc gtgggatgat tataaaaacg
      781 cgcatccgga acaatccgta gatgaacagc ttaaaggcta tcacaaacta atacaagctc
      841 ccgaacgcga agctccggtt catgttccgt tctttagttc tgacggaact ctccgagatc
      901 ttcgtcccgg acagaagatg cctgccggaa gcgttaaagt cgccgattgg gaaaagttaa
      961 acccccagca aaaagaacga ataactcaga tccacaatca actcaaggtc cttaactccc
     1021 cgcagacttt gaaacaacgc tcgatgcagc ggatgttcga tcctgcttgg cagcaagccc
     1081 atcctaatgc aaaaccgggc gaggccggga aatctgtggc agcggatatt caatccgaaa
     1141 tcaatgccct caataaagaa gcccaagata tttcaggctc cgccggtgga aaccccatgg
     1201 gcggcgggca aggaaccgca acctacaacc aagctacagg ccaaat
//
LOCUS       DATMKH010000607         1704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2186996, whole genome shotgun sequence.
ACCESSION   DATMKH010000607 DATMKH010000000
VERSION     DATMKH010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2186996"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1264)
                     /locus_tag="VNV63_07230"
     CDS             complement(<1..1264)
                     /locus_tag="VNV63_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62450.1"
                     /translation="MKSTRLLILIPVLLVLVFFAGWFGLHSFIQSDSFRGWLGKKVGH
                     AIHVDGQFEPLTWEGSSFRSAGFSGAGTAKGKLRSLRVVNIVAHFDWSQLLKGKWVID
                     HISAEKLEALVGKKPAEIRSSPVAPTKESQSQEFPNFFPSELRIEQLYIASADLHWET
                     NHGDTGQLVGAKLTATLRESDQWDVTSVGGNARHASYPALQVDHIRATVSRDSIVIRD
                     AKAAIPGGGEIQLAGKVSTGRQLSAQFTADFSELNAIQALPAEWHVGGKASGHLVYTG
                     DLDRFEHGEIIGTVKITGAAFDMTNLFATLHQLAKFGGLNDVRIDSIETHFKYHEHEL
                     QLSDIQASYQDQIRVEGAGTITPDRLNGNLLIGLSPKILGWIPGAEEKVFVEQRDGLR
                     WATVNISGTPQQPKEDLTKRLISSFRDRM"
BASE COUNT          393 a          449 c          459 g          403 t
ORIGIN      
        1 tcatcctgtc tcggaacgaa ctgatcaatc gcttcgttaa atcttctttc ggttgctgcg
       61 gagttccgct gatgttcacc gttgcccagc gcaaaccatc tctttgctct acaaaaacct
      121 tttcctcggc acccggaatc cacccgagaa ttttcggaga aagtccgatg agtagatttc
      181 cattaagccg atcaggagtg atcgttccgg cgccttccac ccgaatctga tcctggtagc
      241 tcgcttggat atccgatagt tgcaattcgt gttcgtggta tttgaaatgt gtctcgatcg
      301 aatcgatccg gacgtcattc aggccgccga acttcgcgag ctgatgaagc gtcgcaaaaa
      361 gattcgtcat atcaaatgcg gcaccggtaa ttttgaccgt cccgatgatt tccccatgtt
      421 caaatcgatc aagatctccg gtgtaaacga gatgacctga ggcttttcct ccgacatgcc
      481 attcggccgg caacgcctgg atggcgttca gctcagaaaa gtcggccgtg aactgggcgc
      541 tcaactgccg tcctgtggag actttcccgg ccaactggat ttcgccgccg cccgggatgg
      601 cggccttggc gtcccgaatg actatggaat cccggctgac ggtggcccgg atatggtcca
      661 cttgcaacgc agggtaggac gcgtgccgcg catttcctcc gacggacgta acatcccatt
      721 gatcagactc cctcagagtc gccgtcagct tggccccaac caattgaccg gtgtctccgt
      781 gattcgtttc ccaatgaaga tctgcggagg cgatgtaaag ttgttcgatc ctgagttcgg
      841 aagggaaaaa atttgggaat tcctggcttt gggactcctt tgttggggcg actggtgaag
      901 aacggatttc cgctggtttt tttccaacaa gcgcttccag tttttccgcg ctgatatggt
      961 cgattaccca tttgcccttc aaaagctgag accagtcaaa gtgcgcaaca atattgacca
     1021 cgcgaaggga tcgcagtttg cctttggcgg ttcctgcgcc cgaaaagccc gccgatcgga
     1081 aggatgaacc ctcccaagtg agaggttcaa attgaccgtc gacatggatg gcatgaccga
     1141 cttttttgcc gagccaaccg cgaaacgaat cgctctgaat aaaggaatgg agaccgaacc
     1201 acccggcaaa gaataccagc accagtaaga ccgggatgag aatcaaaaga cgagtgcttt
     1261 tcacgcgaac gagataaagg attgcgggat agaattggtc tcagttgcag gcagacgatt
     1321 gtccagacta ttcggtgatc tagccccgac cgcaagcgga gttgtggggc aggggaaggg
     1381 agtcaggacc gccccacggg ccagcgcgcg tgaagatttg agaagaaact tttgcgcggc
     1441 ggctaacttc aagtatcaga gagaggttac aggagtcgga actcaggagt caggagttgc
     1501 aggagttcag gagctagaga atctgggagc ttggcgagga ttttcccagg agatgctggt
     1561 cctgattttc ggataaagac cttgctcgcg tattctgcaa ctcctgcaac tcctgactcc
     1621 tgagttccga ctcctgtaac ctctctgata cttgaagttg gccgccgcgc aaaagtttct
     1681 tctcaaatct tcacgcgcgc tggc
//
LOCUS       DATMKH010000608         2000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_924868,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000608 DATMKH010000000
VERSION     DATMKH010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_924868"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VNV63_07235"
     CDS             <1..265
                     /locus_tag="VNV63_07235"
                     /inference="COORDINATES: protein motif:HMM:NF015440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-alpha domain-containing protein"
                     /protein_id="HXC62451.1"
                     /translation="FLLSRRTGVYFAVIKEGRVAAGDRVEPVYRDKHGVTIADITRLY
                     AFERDDLEMLQRAVQVKTLPEKWRRRFQHQIEEQAKIAAAPPA"
     gene            328..1005
                     /gene="ccmA"
                     /locus_tag="VNV63_07240"
     CDS             328..1005
                     /gene="ccmA"
                     /locus_tag="VNV63_07240"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HXC62452.1"
                     /translation="MAAIQGIQLSKSYHHYQVLKEISLEVSPGDCYVLIGPNGAGKTT
                     LLRILATLQNPSSGRFEIMGHDGVKERDKIREVLLLVAHGSYLYDELNAEENIRFAMG
                     LRGLYPTDHEIKIALDRVGIGAFKDLKTRYFSMGMKRRLSIARATLIRPKVLLLDEPY
                     SSLDENGMTMVNSYIREITQQGASVLLTTHNRVKSAEVGHRAGALVRGELKEIGMKDL
                     TAKDDLL"
     gene            992..1669
                     /locus_tag="VNV63_07245"
     CDS             992..1669
                     /locus_tag="VNV63_07245"
                     /inference="COORDINATES: protein motif:HMM:NF015344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HXC62453.1"
                     /translation="MTFFRVIRLIAWKDLVSEIRSRENISSMFFFALIVILIFSFSFS
                     MDQPAARQFISGIIWVAFAFTGIIGLGKSFSAELQNDCLENLQMSPVSKGAVYLGKLT
                     ANLLFMLIVELILFPMFVIFFNLDVVETLPLVLLIFLLGTLGLSAVGTLFSAMTVQIR
                     TREVMFPILLLPLAIPVMIAAVEATRGALNRDPWAFYGYWIQLLIVYDIVFTVISFWA
                     FEWILES"
     gene            1769..>2000
                     /locus_tag="VNV63_07250"
     CDS             1769..>2000
                     /locus_tag="VNV63_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009381603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C assembly protein"
                     /protein_id="HXC62454.1"
                     /translation="MIKAMRRYEGWFGAAAGVCIIIGLYLGLIASPPDYYQGEVVRIM
                     YIHVPFANSSLLGYTVLFAGSVWYLWKRDPIVD"
BASE COUNT          427 a          512 c          563 g          498 t
ORIGIN      
        1 gtttctgctc agccggcgga ccggggttta ttttgcggtc atcaaagaag gacgggttgc
       61 agccggagat cgggtggaac cggtctaccg ggacaaacac ggcgttacca tcgccgatat
      121 tacccgcttg tacgcgtttg aacgggacga tctagaaatg ctgcaacgcg ccgttcaagt
      181 caagaccttg ccggagaaat ggcgcaggcg gttccagcac cagatcgagg agcaggctaa
      241 aattgcggcg gccccgcctg cttgacaaac cggggacagt caagtagtct tatcgccctt
      301 agtcaatgta cacagacagg tccaagtatg gccgcgatcc aaggaataca gcttagcaaa
      361 tcataccatc attaccaagt tttaaaggag atctctctgg aggtgtctcc cggagactgt
      421 tacgtgctga tcggcccgaa cggggccggc aagacgacac tgcttcgtat acttgcaaca
      481 ttgcaaaacc cttcctcggg acggtttgag attatgggcc atgacggcgt caaggaaagg
      541 gataagatcc gtgaggttct tctactggta gcccatggat cgtacctcta tgatgagctc
      601 aatgccgagg aaaatatccg gttcgcgatg ggcctcaggg ggctataccc gacagatcat
      661 gagatcaaga tcgccctgga ccgcgtcggg attggtgcgt tcaaggatct taagacccgc
      721 tatttctcta tggggatgaa aaggcgcctc tcgatcgcca gggcgaccct catccgtccc
      781 aaggttctgc ttctggacga gccttactcc tccctggacg aaaatggaat gacgatggtc
      841 aacagctaca tccgggagat cacacagcag ggcgcgtctg tcctgctaac gacccacaac
      901 cgtgtcaagt cggccgaggt cggacatcgg gccggagctc tggttcgggg cgagctcaag
      961 gaaatcggca tgaaggatct gacagccaaa gatgaccttc tttagagtca ttcgattgat
     1021 cgcgtggaag gatttggtca gcgagatacg aagccgcgaa aatatctctt cgatgttctt
     1081 cttcgcgctc atcgtcatcc tgatctttag tttcagcttc tctatggacc agccggcggc
     1141 caggcagttc atctccggca tcatctgggt cgccttcgct tttaccggga tcatcggcct
     1201 gggcaagtca ttctcggcag agctgcaaaa cgattgtctg gaaaatctcc agatgagccc
     1261 ggtctccaaa ggggccgttt atcttgggaa actgaccgcc aatctcttgt ttatgctcat
     1321 cgtagagctg attctgtttc caatgttcgt gatatttttt aatcttgatg ttgttgaaac
     1381 actccccctg gttctgttaa tattcttgtt gggaaccctc gggctctcgg ccgtgggaac
     1441 cctcttttcg gctatgacgg ttcagatccg gacccgggag gtcatgtttc cgattctcct
     1501 cttgccactg gcgataccgg tcatgattgc agcggtcgag gctacacggg gcgccttaaa
     1561 cagggaccct tgggctttct atggatattg gatacagttg ctcattgtgt acgatattgt
     1621 ttttaccgta atttcatttt gggcttttga atggattttg gagtcttaaa aaaagcgagc
     1681 gtcaggctcg gcctacgcga gcgcggggtg tgggggcatc ggaggaccgg cattctgttt
     1741 tgccgcacgg gcccccattt ggagatttat gattaaggcg atgcgtcggt acgaaggctg
     1801 gttcggggct gcggccggag tgtgtattat catcggcctg tacctcggac tgatcgcctc
     1861 ccctccggat tactatcagg gggaagtggt gcgcatcatg tacatccatg tcccgtttgc
     1921 gaacagctct cttctgggat acacggtcct ctttgccgga agcgtctggt acctctggaa
     1981 gcgggatccg atcgtggata
//
LOCUS       DATMKH010000609         1155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3156732, whole genome shotgun sequence.
ACCESSION   DATMKH010000609 DATMKH010000000
VERSION     DATMKH010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3156732"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1059
                     /gene="clpX"
                     /locus_tag="VNV63_07255"
     CDS             <1..1059
                     /gene="clpX"
                     /locus_tag="VNV63_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HXC62455.1"
                     /translation="DLHVIGQDSAKKVLSVAVHNHYKRILNANPTKDISLDPYADVEI
                     EKSNILMIGPTGCGKTLLARTLARILDVPFCIADATTLTEAGYVGEDVENIILRLLQN
                     ADYDVKRAEIGIVYIDEIDKIGRKTDNVSITRDVSGEGVQQALLKILEGSSCNVPPQG
                     GRKHPHQEYIQVNTEKILFICGGAFVGLDRLINRRLGNRVLGFHSPQNSASELHLRDT
                     LRHTEPEDLLSFGMIPEFIGRLPIVTALDSLTEEELILILTDTRNAMVKQYTKLLAME
                     GVNLVVTKDALRALAQQAATKGTGARALRSMFEKIMLDVMYDVPSREDIADVVINRAV
                     VEGKRAPLIRRKQDKDAA"
BASE COUNT          279 a          296 c          319 g          261 t
ORIGIN      
        1 gatctacatg tgatcggcca ggactcggct aaaaaggtcc tttcggtggc ggtgcacaac
       61 cattacaagc gcatcctgaa cgccaacccg acaaaggaca tttccctcga tccgtacgct
      121 gacgtcgaga tcgaaaagag caatatcttg atgatcggcc cgactgggtg cgggaagacg
      181 cttctagccc ggacattggc ccgaattctg gatgtgccgt tttgcatcgc cgatgccact
      241 accttgaccg aagccggata tgtcggcgaa gacgtcgaaa acattatctt gcggcttttg
      301 cagaatgcgg actacgacgt caagcgagct gagatcggga ttgtttacat cgatgaaata
      361 gacaagatcg gccgtaagac cgacaacgtc agtatcaccc gcgatgtctc gggtgaaggc
      421 gtccagcagg cgcttctgaa gatcttggaa ggaagcagct gcaacgtccc acctcaaggc
      481 ggacggaagc atcctcatca agagtacatc caggttaata ccgaaaagat cttgtttatt
      541 tgcggaggcg cttttgtcgg gttggatcgg ttaatcaatc ggcgtctagg caatcgggtg
      601 ctcggctttc attcgcctca aaacagtgct tcagagctcc atctgcggga tacattgcgg
      661 cataccgagc ctgaagatct gctctccttc gggatgattc cggagttcat cggccggtta
      721 ccgattgtga cggcgttgga tagcttgacc gaagaagagc tgatcctgat tttgactgac
      781 acgcgcaacg cgatggtgaa acagtacacc aaactgttgg caatggaggg agttaacctg
      841 gtggttacca aggatgctct ccgagccctg gcccagcagg cggcaacgaa aggaaccggg
      901 gcccgagcgc ttcgttccat gttcgaaaag atcatgctcg acgttatgta cgacgttccc
      961 agccgggaag acattgccga cgtagtgatc aatcgagcgg tggtcgaggg gaaacgagct
     1021 ccccttattc gcaggaaaca ggacaaagac gcggcctgat aaaccgcttt cttctagttt
     1081 cacctcaaag gcacagagga cgcagaggcc aattttcggt ttcctttttc agatctttgt
     1141 gtcctctgtg tctct
//
LOCUS       DATMKH010000610         5353 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2851841, whole genome shotgun sequence.
ACCESSION   DATMKH010000610 DATMKH010000000
VERSION     DATMKH010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 5353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5353
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2851841"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..883
                     /locus_tag="VNV63_07260"
     CDS             59..883
                     /locus_tag="VNV63_07260"
                     /inference="COORDINATES: protein motif:HMM:NF041770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HXC62456.1"
                     /translation="MTIPVAAANCQTTSTPPTCTQTLSLTLTAPSLSVAFGNQELILS
                     WSTPLPSGFNIVTGYNVYYMEQGGSTWTKISVGNVNSYTLTGLTNGTTYDVKVTAVAS
                     PMYFINVTALDDYTTPHESHLLVPDVSAPINCSIPSCESPGSNTATNSPQPIAPFPDL
                     PDQGHCFIATAAYGSQWEPQVKILRAFRDEYLEHYNLGRRFIAWYYHASPPWAQYLNE
                     HVWLKPMVRVVLIPAVGFAYFFVTATGTERLLIFLLVALGLIGGTFVVEKSRRSRD"
     gene            880..1524
                     /locus_tag="VNV63_07265"
     CDS             880..1524
                     /locus_tag="VNV63_07265"
                     /inference="COORDINATES: protein motif:HMM:NF040596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MXAN_2562 family outer membrane beta-barrel
                     protein"
                     /protein_id="HXC62457.1"
                     /translation="MNIIRGILFVLLILFITASSTQAAAFPSWSFSLKAGAYQPNAAG
                     YDINYGDPRAVRGDLELGYKITRRIEVGLSVGYFMDNGLALGAVSGTPSGVKQKLVLI
                     PTQLYLVYQFAFNDGQIFVPYLGGGYTHITYRRSVEGQGTIMGGKDGYHARAGLKFLL
                     NRLEPSTADKLYQTSGIMNTYFLLEGQYARVNGFDDSSVDLGGWSYFGGLQFEF"
     gene            complement(1451..2515)
                     /gene="pdxA"
                     /locus_tag="VNV63_07270"
     CDS             complement(1451..2515)
                     /gene="pdxA"
                     /locus_tag="VNV63_07270"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HXC62458.1"
                     /translation="MKPVIGITMGDPVGIGPEIIIKSLSRARIHVLCRPFVLGDSRLF
                     EKTIRRLRSPLKIRGISNLEDLRFSGKILNVLSLPIEDLDYLKPGVPNKSAGMAMLAA
                     INKGTEMALKGEIAALVTAPIHKEALRLTGSPHPGHTELLANLSDLGGGKPSVRMMLV
                     GGKLRVILVTTHAALSEVPKLITTPKVFTTIQLADSTMKKHFGIRRPRIAVAGLNPHA
                     GEGGLFGQEDQALIRPAVEQAKAGGIQAVGPLPPDSLFHQAYRGDYDVVVVMYHDQGL
                     IPLKMLSFGHAVNITVGLPFIRTSVDHGTAYDIAGKGIADPSSLIAAIELAVKMVKTQ
                     TGGRQSNSIHPDPLKNRQSR"
     gene            complement(2512..3396)
                     /locus_tag="VNV63_07275"
     CDS             complement(2512..3396)
                     /locus_tag="VNV63_07275"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HXC62459.1"
                     /translation="MTTTYKQVKNILSLTLFLNLLVAVIKLLWGRWTNSLSMQADGFH
                     SLFDGASNIIGLVGIWAARHPPDDCHPYGHKKFETFAAFGISVFLFVTCFSILENSYA
                     RFQTGVVPDVPGLSFLIMLATMGINLFVTWYEQKQAVALKSGILQADSMHTLSDVYSS
                     LTVLIGLAAVRLGLPILDSIMAVAIAGFIGRTGFKILFESSRVLSDASRVDPRLVKEV
                     VMQVPGVKSCHSIRTRGLENHVFVDCHIYVQPDMTADKTHDLVHEIEDLIKKEMSEVV
                     DVVIHVEPDVQHTPHAIS"
     gene            complement(3545..4465)
                     /locus_tag="VNV63_07280"
     CDS             complement(3545..4465)
                     /locus_tag="VNV63_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HXC62460.1"
                     /translation="MSYQVLGWIVFNIFVLAMLALDLGVFHRTAHVVKLKEALLWSAV
                     WVSLALLFNLGIFFWRGPVTALEFLTGYLIEEALSVDNLFVFLLIFSYFRVPSLYQHK
                     ILFWGILGALIMRAVFIVAGITLVQKFHWIIYLFGAFLVLTGTKMALQKDKGIHPEKN
                     PVLRLFQRFMPVTDRYEEGSFFVSRAGRYLATPLFVVLLVVETTDVIFAVDSIPAVLA
                     ITLDPFIVYTSNIFAILGLRSLYFALAGMAALFHYLHYGLSAILVFVGTKMLLADIYK
                     IPVGVALGVVACFLMISVIASIVHPRKKEV"
     gene            complement(4465..>5353)
                     /gene="larC"
                     /locus_tag="VNV63_07285"
     CDS             complement(4465..>5353)
                     /gene="larC"
                     /locus_tag="VNV63_07285"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HXC62461.1"
                     /translation="RLARAEAKVHGKQTRTVRFFEIGHLDTIVDVAGAILGLKLLGIE
                     KIFSSSINTGTGLVRTDHGILPVPPPITAELLKGIPILSSDIRHELATPTGTAILTTL
                     AEEFGSLPIMTVAAVGYGAGSHDLVTQPNVLRILIGDRQDHWMEEDRVVLIETNIDDM
                     NPQIYDHLMARLFAHGALDVYLTPIQMKKGRPATLLSILSEKDRVQELTEIVFAETTT
                     LGLRLQEMARRKLPRRTRSVRTPWGNVHVKVATMGEKVIKIIPELDHCKRIAEENGIP
                     LAKILEAVHKSFGKSVKGA"
BASE COUNT         1312 a         1359 c         1395 g         1287 t
ORIGIN      
        1 tgataaccaa gtccacacca taacaaacga tattccagat cctccctata gtaatatctt
       61 gacgatacct gttgcagccg ccaactgcca gacaacgtca acaccaccca cctgtaccca
      121 aaccctttct ttaacgctta cggcaccaag tcttagcgtc gcttttggaa atcaagagct
      181 cattctgagt tggagtacgc cattgccatc aggttttaac attgtgactg gttacaacgt
      241 atattacatg gaacagggag gctctacctg gacaaaaatc agtgttggca atgtcaattc
      301 ttatacgctg acgggtttga ctaatggcac aacgtatgac gtaaaggtga ctgcggtagc
      361 ttcaccaatg tattttatca acgtaactgc tctggacgat tatacgaccc cgcatgagag
      421 ccacttactc gttccggatg tctccgctcc cataaactgt agtattccgt catgcgagag
      481 tcctggttca aatacggcaa ctaattctcc ccagcccatc gcgccctttc cggatcttcc
      541 ggatcagggc cactgtttca ttgcaacggc cgcttacggc tcgcagtggg agccgcaggt
      601 caagattctc cgcgcttttc gggacgaata tcttgagcat tataatctgg gccggcgatt
      661 tatcgcctgg tactatcatg ccagcccgcc gtgggcgcag tatctgaacg agcatgtctg
      721 gctgaaaccg atggtccggg ttgttctgat ccctgcggtt gggtttgctt atttctttgt
      781 gacagcgact gggactgaga ggctcttgat ctttctttta gtggcgttag gtttgatcgg
      841 gggaacgttt gtcgtggaaa aatcgagacg gagcagagat tgaacatcat ccgagggatt
      901 ctgttcgtgc tcctgatcct gtttatcact gcttcgtcaa ctcaagcagc ggcgtttcct
      961 tcctggtctt ttagcctcaa ggcaggcgcg tatcaaccca acgcggccgg ttacgatatt
     1021 aattatggtg atccaagagc ggtccggggc gatctggaat tagggtacaa aatcacgcgt
     1081 cggatagaag tgggtttgag cgtcggctat tttatggaca atggccttgc gctcggtgcg
     1141 gttagcggga ccccgagtgg cgtaaagcag aaacttgttc tgattcccac gcagctttac
     1201 ctcgtctatc aatttgcatt taatgacggc cagatttttg tgccctatct tggcggcggt
     1261 tatacacaca tcacgtatcg ccgctcagtg gaaggccagg gtacgataat gggagggaag
     1321 gatggttatc atgcgcgggc cgggttgaaa tttctgctga accggctgga accctctacc
     1381 gcagataagc tctatcaaac ctcagggatc atgaacacct attttcttct ggaaggacag
     1441 tatgcacgcg tcaacggctt tgacgattct tcagtggatc tgggtggatg gagttacttt
     1501 ggcggcctcc agtttgagtt ttaaccatct tcaccgcaag ttcaatagct gcgatgagac
     1561 tggagggatc agcaattcct ttacctgcga tatcatacgc tgtaccatga tccaccgagg
     1621 tgcgtataaa aggaagcccc accgtgatgt tgaccgcatg gccgaacgaa agcatcttga
     1681 gcgggatcag gccctgatca tggtacatca cgacgaccac gtcgtaatct ccccggtagg
     1741 cctgatggaa aagactgtcc gggggcaacg gtccgaccgc ctgtatgccg ccggctttcg
     1801 cctgctccac ggcagggcgg atgagcgcct ggtcttcttg tccgaataag ccgccttctc
     1861 ccgcatgcgg gtttaagccc gccacggcga tacggggcct gcggattcca aaatgttttt
     1921 tcatcgtgct gtccgctagc tgaattgtgg tgaagacttt gggggtcgta atcaattttg
     1981 gaacctcact caaggcggcg tgcgttgtga ccagaatcac ccggagcttt ccgcccacga
     2041 gcatcattcg gactgaaggt ttgcctccgc ctaagtcgga gagattggcc agcaactcgg
     2101 tatggcctgg atgaggcgat cccgtcagac gcaaagcttc tttatgaatg ggcgcagtga
     2161 cgagagcggc gatctcgcct tttaatgcca tctccgtgcc cttgttaata gctgccagca
     2221 tcgccatccc ggcagacttg tttggcacgc ctggttttaa ataatcgaga tcctcgatcg
     2281 gcagggacaa cacgttcaga attttcccgg aaaacctgag atcttccaga tttgaaatgc
     2341 ctctgatctt caggggagaa cggagccgtc ggatcgtttt ttcaaacagg cggctgtcac
     2401 cgaggacgaa aggccggcag aggacatgga ttcgtgcgcg gctcaggctc ttgatgatga
     2461 tctccggtcc gatgccgact ggatctccca ttgtgatccc gatgacaggt ttcatgagat
     2521 ggcgtgagga gtatgttgaa cgtcgggctc gacgtgaatt accacatcca cgacctccga
     2581 catctctttc ttgattaagt cttcgatctc atgtacgaga tcatgggtct tatcggccgt
     2641 catgtccggt tggacataaa tatgacagtc cacgaagacg tggttctcta gaccccgtgt
     2701 gcggatgctg tgacaggatt tgactccagg gacctgcatt acaacctctt tgaccagccg
     2761 gggatctacg cgggatgcgt cgcttaagac gcgtgagctt tcgaatagta ttttgaaccc
     2821 tgtccttccg ataaagcctg cgatcgcaac agccataatc gaatccagaa tgggcaggcc
     2881 cagcctaacg gctgcgagac cgatcagaac cgtgagggat gagtagacat cactcagagt
     2941 gtgcatcgaa tcggcctgta agatcccgct cttgagtgcg acggcctgtt tctgctcata
     3001 ccatgttacg aacaggttga tccccattgt ggccagcata ataaggaagc tgagaccagg
     3061 tacatcgggg acaacgcctg tctggaatcg agcgtaacta ttttcgagaa tgctgaagca
     3121 agtaacgaat aggaagaccg agataccgaa tgcggcaaag gtctcaaact tcttgtgccc
     3181 gtaaggatga caatcatccg gagggtgacg agccgcccag atccctacaa gcccgatgat
     3241 gttagaggcg ccgtcaaaga gggaatgaaa cccgtccgct tgcatgctca agctgtttgt
     3301 ccaacggccc cagagaagct tgatcaccgc aaccagtaag ttgagaaaaa gtgtcaggct
     3361 gaggatgttt ttaacttgtt tgtatgtggt cgtcatccgc tacttacgat cataccattg
     3421 tttagacggg gaaatctttt ttgttaactt ttcaagcttc gcatgcagca aaaaagcccc
     3481 acgatgccca gaataataaa aaaccatccg gcgatataga caaggattct ttttgcatgt
     3541 tttttcacac ctctttcttg cgcggatgaa cgatggatgc aatgacagag atcattaaaa
     3601 agcaggcgac cacacccaaa gcgacgccta ccgggatctt atagatatcg gcaagcagca
     3661 tcttcgttcc cacgaaaaca agtatggcgg aaagtccgta atggaggtag tgaaaaagcg
     3721 ctgccattcc agccaatgca aagtaaagcg accgcaagcc caagatcgca aatatatttg
     3781 atgtataaac gatgaatgga tcgagtgtga tggccaggac ggcggggatc gagtccaccg
     3841 caaagatgac atcggttgtt tcaacgacca aaagtacaac gaacaacggc gtggcgagat
     3901 aacgccccgc cctgctcaca aaaaaactgc cctcttcata gcggtctgtg accggcataa
     3961 atctttgaaa aagcctgagc acgggatttt tttcgggatg gatccctttg tccttctgca
     4021 gagccatctt ggtgccggtc agaaccaaaa atgcgccgaa gagatagatg atccagtgaa
     4081 acttctgaac cagcgtgatg cccgcgacga tgaagaccgc tcgcatgatc agggcgccca
     4141 gaatgcccca gaaaagtatc ttgtgctggt aaagagaggg gacgcgaaag taagagaaga
     4201 tcagcaaaaa gacgaagaga ttgtctacgc tgagagcttc ctcgatcaga taaccggtca
     4261 gaaattcaag cgccgttaca ggaccgcgcc agaaaaatat tccaagattg aagagaaggg
     4321 ccagggaaac ccataccgcg ctccacagga gcgcttcttt gagtttaacc acatgggcgg
     4381 tgcgatgaaa gaccccgagg tccagggcca gcatcgcaag gacaaagata ttgaagacaa
     4441 tccaccccag gacttggtat gacatcaagc gcccttcacg ctttttccaa agctcttgtg
     4501 aaccgcttcc agtattttcg caagaggaat accgttctcc tcggcgatgc gtttacaatg
     4561 atcaagttcc ggtattattt ttatcacctt ctctcccatc gtggcgactt ttacatgcac
     4621 atttccccag ggagttctga ccgaccgagt tctacgcggg agctttcttc gtgccatttc
     4681 ttgtaaccga agacccagag tggtggtctc ggcaaagacg atctccgtca gttcttgaac
     4741 cctgtctttc tccgagagaa tgcttagcaa ggtggcaggc ctcccttttt tcatctgaat
     4801 aggggtgaga tagacatcca aggctccatg ggcaaagagt cttgccatta gatgatcata
     4861 aatctgggga ttcatatcgt caatattggt ctcaatcaat acgacccgat cttcttccat
     4921 ccaatgatct tgccggtcgc cgatgagaat ccgcaggaca tttggctgag tcacaaggtc
     4981 atggctcccc gcaccgtatc cgaccgccgc cacggtcatt atcgggagag acccaaactc
     5041 ttccgcaaga gtcgtaagga ttgccgttcc ggtgggcgtg gcgagctcat gacggatatc
     5101 ggaggagagg ataggaatgc cttttagaag ttcagcagtg attggtgggg gaacagggag
     5161 aataccgtgg tcggtgcgga caagaccggt gccggtgttg atggaggagg agaagatctt
     5221 ttcgatgccg aggagtttca atcccagtat cgctccggcc acgtccacga tcgtgtccag
     5281 gtgaccgatc tcgaagaaac gcaccgtccg ggtctgttta ccgtgtactt tagcctccgc
     5341 ccgtgccaga cga
//
LOCUS       DATMKH010000611         3724 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4120917, whole genome shotgun sequence.
ACCESSION   DATMKH010000611 DATMKH010000000
VERSION     DATMKH010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3724
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4120917"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1931)
                     /gene="acs"
                     /locus_tag="VNV63_07290"
     CDS             complement(<1..1931)
                     /gene="acs"
                     /locus_tag="VNV63_07290"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006669802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HXC62462.1"
                     /translation="MAQADIESVLRERRIFHPKPAFSRQAHIKSFKQYRQLYQKAAKN
                     PEAFWAGIAKELHWFKKWRKVLVWDRPFAKWFVGGKINASYNCLDRHLTTWRKNKAAL
                     IWEGEPGDSRVLTYQDLHREVCRFANVLKSFGIRTGDRVAIYMPMVPELPIALLACAR
                     IGATHSVVFGGFSAEALKDRINDAKAKLLITADGGFRKGAVVPLKENVDKALTDAPSI
                     EKVIVYQRTKTPVPMMAGRDYWWHEVIQKTSADCEAMPLDPEHPLFILYTSGTTGKPK
                     GVVHSTGGYLLGVYITSKWVFDLKEEDTYWCTADLGWVTGHSYIIYGPLANGVTSLMF
                     EGVPTYPNPDRFWQIIEKYRVNVFYTAPTAIRALIKTGEEWPNKHDLSSLRLLGTVGE
                     PINPEAWMWYHRVIGKGRCPIVDTWWQTETGMILITPLPGAIPTKPGSGTLPFPGIAI
                     DVVNKKGESAAPNQGGYLVIKKPWPAMLRTIYGDPERYKKQYWSEIPGCYFTGDGARK
                     DKDGYFWIMGRVDDVINVSGHRLGTMEIESALVSHPAVAEAAVVGKPDEVKGTAISAF
                     VTLEARHSPSDQLKEELRQHVVGQIGALARPDEIRFTDNLPKTRSGKIMRRLLRDIAS
                     GSHKIGDTTTLEDYSVLTKL"
     gene            2227..2616
                     /gene="dksA"
                     /locus_tag="VNV63_07295"
     CDS             2227..2616
                     /gene="dksA"
                     /locus_tag="VNV63_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HXC62463.1"
                     /translation="MVNTKKAKKEPKKYEEIKKELERQKTALLAEVGEIITGGISPGT
                     ENFPDLGDQATAETDQNFLLRLKEREQKLLKKIDEALDRIKNSTFGICESCGKEIGFK
                     RLKARPVTTLCIDCKTQQEAEEKIREA"
     gene            complement(2659..3171)
                     /locus_tag="VNV63_07300"
     CDS             complement(2659..3171)
                     /locus_tag="VNV63_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62464.1"
                     /translation="MAKKGYGFILFVAMISGAIGGIVGGRFFMSDPVFAAKAPKPQKA
                     IIAEKFVIVDENGVSRAVLGQYSKNKVAVGRVALTLQDEKGNARAELAVTKEGITGLN
                     FFNKDGKPRASLVFSDDGGPVLSFGSKEGDSHVQLIIDNDGSPHFLLDGGMPGIDMFD
                     KDGELLWSAP"
BASE COUNT          859 a         1059 c          945 g          861 t
ORIGIN      
        1 agcttcgtca ggaccgagta atcttccagc gttgtggtgt ctccgatctt gtggctgccg
       61 gaggcgatat cgcgcaacaa tcgccgcatg atcttcccgc tccgggtctt gggaagattg
      121 tcggtaaatc ggatctcatc cggccgggcc agtgctccga tctgtcccac gacgtgctgc
      181 cgcaactctt cttttaactg gtccgaaggc gagtgcctgg cctctagtgt gacaaaggcc
      241 gagatcgcgg tgcccttgac ctcatccggc ttgcccacca cagcggcctc ggccacggcc
      301 ggatggctca ccagggcgga ttctatctcc atcgtgccca atcgatggcc cgacacgttg
      361 attacatcat ccacacgtcc catgatccag aaataaccgt ctttatcttt ccgcgctcca
      421 tccccggtga agtagcatcc cggaatctcg ctccagtact gttttttgta gcgttccgga
      481 tctccataga tcgtccggag catcgcgggc cagggtttct tgatcacgag atagccgccc
      541 tgattggggg cagcggattc acctttcttg tttaccacat ctatcgcgat ccccggaaaa
      601 ggcaatgtcc cggagcccgg cttggtcggg atcgcaccag gcagcggagt aatcagtatc
      661 attccggtct cggtctgcca ccaggtgtcc acgatcgggc agcggccttt cccgatcaca
      721 cggtggtacc acatccatgc ctccggattg atcggctcgc ccaccgtgcc gagaagcctt
      781 aggctcgaca gatcgtgctt gttcggccat tcctctcccg ttttaatcaa ggcccgtatt
      841 gcggtcgggg cggtatagaa gacattcaca cgatacttct ctataatttg ccagaagcga
      901 tccggattcg ggtaggtcgg caccccttca aacatcaggc tggtcacacc gttggccagc
      961 gggccataga taatgtagct gtggcccgtg acccatccga gatcggccgt gcaccagtaa
     1021 gtgtcttctt ctttaaggtc aaagacccac ttgctcgtga tgtacacccc tagcagatag
     1081 cccccggtgg aatggacgac gcccttgggc ttacccgtgg tcccgctcgt gtagagaata
     1141 aacaacggat gctccggatc cagtggcatc gcctcgcaat cggccgaggt tttctggatt
     1201 acctcatgcc accagtagtc ccgacccgcc atcatcggca cgggcgtctt ggtccgctgg
     1261 tacacgatca ccttttcgat gctgggcgca tcggtaagcg ctttgtccac attttctttc
     1321 aaggggacaa ccgctccctt tcggaatccg ccgtccgcgg taatcagaag cttcgccttg
     1381 gcatcgttga tccggtcctt gagcgcctcg gccgagaacc ctccgaagac cacgctgtgc
     1441 gtcgccccga tccgggcaca ggccagcagc gcgatcggga gttccgggac catcggcata
     1501 tagatcgcga cgcgatcacc cgtacggatc ccaaaggatt ttaggacatt ggcaaagcgg
     1561 cagacctccc gatggaggtc ttgataagta agcacgcggg agtcgccggg ttccccctcc
     1621 cagatcaggg cggccttgtt cttccgccag gtggtcaaat gtcggtccag acagttgtac
     1681 gaagcattga tcttgccgcc cacgaaccat ttggcaaaag gccgatccca gaccagaacc
     1741 ttcctccatt tcttgaacca gtgaagctcc ttcgcaatgc cggcccaaaa ggcttccggg
     1801 tttttcgcgg ccttctggta cagctgccga tactgtttga agctcttgat atgggcctgt
     1861 cggctaaaag ccggtttagg atggaaaata cgacgctctc tcaagaccga ttcgatatct
     1921 gcctgggcca tgtcgttcgc ctccccgcga tcctgaatga tctgatcctg gtttgattaa
     1981 gggaaaatca ttttagtgga aagctcagag gcttgtcaac acgcctagcg cgccggaatc
     2041 tcctggtttt ctcaggagtc aaggcgcgcg gataccacta gtttgagttc catccggatc
     2101 gaacgatccc cctgcttact aggggttctt caacaattgg gattgacttg cagtgaaaca
     2161 ttctgatata gtgagcagct aattttacga ccagttacag ctgtggaggc ttagggagaa
     2221 ttggtcatgg tcaatacaaa gaaagccaag aaagaaccaa agaaatacga agaaatcaag
     2281 aaagaactcg aacggcaaaa gactgccctg ttggcagagg tcggagagat catcacgggc
     2341 gggatcagtc cgggcaccga aaattttccg gacctgggag atcaggccac tgccgagact
     2401 gaccagaact ttctcctgcg tttaaaggaa cgagaacaga aactcctaaa aaagattgat
     2461 gaggccctgg accgaatcaa gaatagcacc tttggcattt gtgaaagctg cgggaaagag
     2521 attggattta aacgcctcaa ggcccgaccg gtgacgacgc tctgtattga ctgcaagacc
     2581 caacaagagg ctgaagagaa gatccgggag gcttaaagag ggaacctgtg atgggcgatg
     2641 ctgggattcc agcaacctct acggtgccga ccagagtagc tctccatcct tgtcaaacat
     2701 gtctatgcct ggcataccac catcgagcaa aaaatgaggg cttccatcgt tatcaatgat
     2761 aagttgaaca tgggagtctc cttctttact gccaaaggat agaaccggtc caccatcatc
     2821 cgaaaacaca agtgaagcgc gaggcttgcc gtccttgttg aagaagttca ggcccgtaat
     2881 cccttctttc gttaccgcca gttcggcgcg ggcattgccc ttctcatctt gaagggtcag
     2941 ggcgaccctt cctactgcca ctttattttt actgtattgc cctaacacag cacggctcac
     3001 accattctca tccacgatca caaatttctc ggctatgatg gccttctggg gctttggcgc
     3061 cttcgcggca aacaccggat cgctcatgaa gaatcggcct cccactattc cacctatcgc
     3121 gccagagatc atcgccacaa acaaaataaa cccgtaacct tttttagcca tctgtatacc
     3181 ccctttccaa tgcacagtct gccacaatga attttagccc ccgttaaacg gattagtcaa
     3241 tcatctttga ttgacataaa cgcacaaatt gagtaagcta gtccgatttt tcagccgagt
     3301 aaaagcgagt aaggccgagt aaagcgcata aagcagtttg tttttactga ggatcttcga
     3361 ttaagacgaa taaacatcaa accctcctct ttatacagaa aaaaggtgcg gttcgagccc
     3421 aggatcttgt tcgccagttc gactattctc cgggaacgtc taggtcttac ctcagctacc
     3481 taatgcggca aaatctctta gagcggactg cccatgggca tgtcttgaca ggcacgggac
     3541 agagccgtct gcaattcttt gaagctatgg gttgtggtaa cccaggctgc cccctttgcg
     3601 aggcgaagaa ggcagagcac ttcacttgcc caacctgtcg ttggcagcta cccaaaaaga
     3661 aagcccgcat cggccctgcg tggagcccgc cattttttcg ttgggagaac ggggtctatt
     3721 gccc
//
LOCUS       DATMKH010000612         1128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1875083, whole genome shotgun sequence.
ACCESSION   DATMKH010000612 DATMKH010000000
VERSION     DATMKH010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1875083"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
BASE COUNT          283 a          326 c          274 g          245 t
ORIGIN      
        1 gccgtcggac cactaggtta tatcattgtc gacaccccat cctacggaca aatcgttcgc
       61 gtggccgatt acacagctcg ctacatcgga ctctaaagat agcttcgccg actctttccg
      121 tctcaggatt ccagactaaa aattaccact gggaatgtaa tcaaccgcgc cggacccaga
      181 gggtgccgta ccagcaggct gcggtggagt tccagtgtcc tgaagttgaa cacctccaaa
      241 gcccgcaccc cagccggagt tctccgcgca tcctgccaga agtatcacca tgcaaaaggc
      301 aagaaggcct aggcctaaag tcaaatagcc tttcatggat gaaataatta acattcggct
      361 tgcccatctc aagtagttcc cacagagatt aggcgaaaga tcgccgaaaa ttcggagaga
      421 tgcccgcctc tcttgcgttc ccaccctcct ttgctgaaac ttgccagtct tctgaatctc
      481 ctatgccgcc cggtgatatt tacgtgcaaa tgacggcctc ctccgaatcc tccggaaagt
      541 cgagatcagt ggttgttctg gtacctcgcg cgaacgtaga gaatttcaga aggcaaaaga
      601 acgattcaaa atccgccgac cacttcatcg ccacttcgct tgccgagcct ctggctgaat
      661 atgctttcac tcaccgaacg attttttcca cgttcgaatt aactcactca ttctccgcag
      721 ttcctccacc cgaaattgaa ggcaggcccc caaatatatc gcagggcggt ctgaaagcct
      781 ggatcgtgtc gtgaggggta tccagttcat tgaccggtct ccaaagaatc cgatgaggac
      841 gagttcgagt acgaagatga ttttaggaac gattgcgagg aaacagcttt gtcggatcga
      901 acggagattg cgtcgggatc gaacaggccc gttcgatcca tgattccgga gcggaggtgt
      961 tctcacccag ccgaaagcac ccgtcgccgg gccgcataat acgaaccgat gaccaggatc
     1021 gcggcgagca actgggcgac cagcgtctct acggtcggaa agatagagaa ccataccccc
     1081 atccaatccg gaataacact ttcgagcgcg ccgattttcg tcgtcggg
//
LOCUS       DATMKH010000613         2505 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_285812,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000613 DATMKH010000000
VERSION     DATMKH010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285812"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..155
                     /locus_tag="VNV63_07305"
     CDS             <1..155
                     /locus_tag="VNV63_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HXC62465.1"
                     /translation="VTGTGKIMRRRASKSHLLTKKTRHKKSAMKKTTVVSRADHRTIE
                     RLIPYA"
     gene            185..565
                     /gene="rplT"
                     /locus_tag="VNV63_07310"
     CDS             185..565
                     /gene="rplT"
                     /locus_tag="VNV63_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003248692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HXC62466.1"
                     /translation="MPRAKGGPKTKQRRKKRLKLAKGYWGAKSKLFRTATEAVDKSGL
                     YAYRDRKNRKRAFRRLWITRINAAARIMGMSYSQFMSGLKKAGIGLDRKVLADMAVQD
                     QPSFDKLAEMAKEKTKVKEKTAVS"
     gene            complement(562..2247)
                     /gene="pheT"
                     /locus_tag="VNV63_07315"
     CDS             complement(562..2247)
                     /gene="pheT"
                     /locus_tag="VNV63_07315"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HXC62467.1"
                     /translation="MPTITVKKKDLETLVGKQLPVETLSRYLEWVKGETKEYSPATDD
                     LRIELNDTNRPDLWSCAGIARQIRAKLNSRPPVYPFFRNGKKKHPQLLVSKELKIIRP
                     YIGACLAKGLTITEETLVQLIQTQEKLADNFGRKRRMVSIGLYRLELIKFPVSYTAVE
                     PAACSFVPLGMETPMTLQAILEQHPKGTEYAAILAGAPRYPLLMDRDQKVLSFPPIIN
                     SREIGEVKVGDRDLFVEVTGTDLRMVIVAINILAADLSDRGATIEPVETRFPFETAFG
                     EKVVMPYDLSSPVTVSLAEVESALGEKVTKTELKQLLVSYGYQLKTRGNRFEAKPPLY
                     RDDVMHPVDLIEDVAISRGFDSFRPVMPERATVGGLSAQEFFSDQVREMMVGFGFQEI
                     ISNILCSRTELADRMGLPGIELVEIENVMSQQYNALRNSVLPSLLRVEAASSKAFYPH
                     RIFEMGEVVKRDPGSKQGSRDMRCLGAFLSHPAANFSELHSFLDLLFYYLGKEYRLEP
                     VVHPSFIEGRVGRILVREKECGVIGELHPQVLENWQIAIPCTVFEVELNPLLR"
     gene            complement(2257..>2505)
                     /gene="pheS"
                     /locus_tag="VNV63_07320"
     CDS             complement(2257..>2505)
                     /gene="pheS"
                     /locus_tag="VNV63_07320"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314942.1"
                     /note="catalyzes a two-step reaction, first charging a
                     phenylalanine molecule by linking its carboxyl group to
                     the alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; forms a heterotetramer of
                     alpha(2)beta(2); binds two magnesium ions per tetramer;
                     type 2 subfamily; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HXC62468.1"
                     /translation="YFPYTEPSVELHVKHPKLGWIELGGAGLFRPEVTQPLGVKVPVI
                     AWGLGLDRMAMVALDIHDIRDLFTADLEMIRAKRTASL"
BASE COUNT          631 a          706 c          633 g          535 t
ORIGIN      
        1 aggtcaccgg aaccggaaag atcatgcggc gcagagcatc gaagagccac ctcttgacca
       61 agaagacgcg ccacaagaaa agcgccatga agaaaacgac ggtcgtgagc cgcgccgatc
      121 accggaccat tgaacgtcta ataccgtacg cttaacctaa atattaatat taaggagagt
      181 tgccatgcca cgagctaaag gtggaccaaa aacaaaacaa cgaagaaaga aacgcttaaa
      241 gctggcgaaa ggctactggg gcgcgaagag caagcttttt cggaccgcta ccgaggcggt
      301 cgacaagtcg gggctttatg cctatcggga tcgaaaaaat cggaagcgcg ctttccgcag
      361 gctctggatc accaggatca atgccgcggc acggattatg ggaatgtctt actcccagtt
      421 tatgtccggg ctcaagaagg caggcatcgg cctggatcgt aaagttcttg ccgatatggc
      481 ggttcaggat cagccgagct tcgacaagct cgccgagatg gcaaaagaaa aaaccaaggt
      541 gaaagaaaaa accgcggtga gttaacgaag gagagggttt agttccacct caaacactgt
      601 gcatggtatg gcgatctgcc agttctcaag gacctgggga tggagttcgc cgatcacacc
      661 gcattctttt tctctgacca agattctccc gaccctgcct tcgataaaag agggatgaac
      721 cacaggctcc aggcgatact ctttccccag ataataaaac aagaggtcca gaaaagaatg
      781 gagctctgag aaattcgctg cgggatgact cagaaacgcc ccgaggcatc gcatgtcccg
      841 gctcccctgt ttggatccgg ggtctcgctt tacaacctct cccatctcaa agatccggtg
      901 agggtagaat gccttggagc tcgccgcctc gacccgaagt agagaaggca gaaccgagtt
      961 tctcaaagca ttgtactgct gggacatcac gttttcgatc tccaccagct cgatcccagg
     1021 aaggcccatc cgatccgcca gttcggttct cgagcagagg atgttcgaaa tgatctcctg
     1081 aaatccaaaa cccaccatca tttcacggac ctgatccgaa aaaaactcct gcgcggataa
     1141 accgcctacc gtggcccgtt ccggcatgac cggtctaaaa gaatcaaagc ctcggctgat
     1201 cgccacatct tcgatcaaat ccacgggatg catcacgtcg tcccgataca gcggcggctt
     1261 cgcttcaaac cgatttcctc tggtttttag ttggtaaccg tacgagacca ggagctgctt
     1321 cagttccgtt ttagtcacct tctcacccaa cgcagactcg acttcggcga gagacaccgt
     1381 tacgggagaa gaaaggtcgt acggcatcac cactttttcc ccgaaagcgg tttcaaaagg
     1441 aaaccgggtc tcgaccggtt cgatcgttgc accgcgatcc gagagatccg ccgccaagat
     1501 gttgatcgcc acaatcacca ttcggagatc agtgccggtc acctccacaa agagatcccg
     1561 atcacccacc tttacctctc cgatttcccg gctgttgatg atcggcggga aagacaaaac
     1621 cttctgatcc cgatccatca gcaacggata gcggggtgcc ccagcaagga tcgccgcata
     1681 ttctgtgccc tttgggtgtt gctctaaaat cgcctgaagg gtcatgggcg tctccattcc
     1741 cagaggaacg aacgagcagg cagcgggttc aaccgcggta taggacaccg gaaactttat
     1801 aagctcgagc cgataaagac cgatcgaaac catcctccgc ttccgcccga aattatccgc
     1861 aagcttttcc tgcgtctgaa tgagctggac cagtgtctct tccgtaatcg tcagaccttt
     1921 cgccaggcaa gccccgatat aaggccgtat gatcttcagc tctttcgata caagcagttg
     1981 aggatgtttt ttcttcccgt ttcgaaaaaa ggggtaaact gggggccggc tgttgagctt
     2041 ggctcggatc tggcgggcga tgcccgcaca gctccagagg tccgggcgat ttgtgtcgtt
     2101 tagttcgata cggagatcat ccgtcgcggg actgtattcc tttgtttctc ctttgaccca
     2161 ttcaagatac cgcgaaagcg tctccactgg gagttgcttt ccaaccagtg tctccaggtc
     2221 ctttttctta actgtgatcg tgggcatttg agagatctat aagctcgctg ttctcttcgc
     2281 ccgaatcatt tcgagatccg ccgtgaaaag gtcccggatg tcatggatat cgagtgccac
     2341 catcgccata cggtcaagac cgaggcccca ggcaatcacc gggaccttca cccccaaggg
     2401 ctgcgtcacc tccggacgaa acaatccggc ccctcccaac tcgatccagc ccagcttggg
     2461 atgcttgaca tgaagctcca ccgaaggttc tgtatacgga aaata
//
LOCUS       DATMKH010000614         1078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4122307, whole genome shotgun sequence.
ACCESSION   DATMKH010000614 DATMKH010000000
VERSION     DATMKH010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4122307"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1078
                     /gene="glgB"
                     /locus_tag="VNV63_07325"
     CDS             <1..>1078
                     /gene="glgB"
                     /locus_tag="VNV63_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HXC62469.1"
                     /translation="AKSNSLDSPISAYELHLGSWRRVPEEGNRWLTYREMAPLLADYV
                     RETGFTHVELLPVMEHPFDGSWGYQTTGYFAPTSRFGTPDDFRYLVDCLHQNGIGVIL
                     DWVPAHFPKDETGLGYFDGSHLYEHGDPRQGEHPDWNTFVFNYGRNEVQTFLISNALF
                     WFDKYHVDGLRVDAVASMLYLDYGRREGEWIPNRYGGRENIEAIHFLRLLNEHVYAAF
                     PDVMMIAEESTAWPQVSRPTYVGGLGFGLKWNMGWMHDVLGYMSKDPLFRSYDHNKIT
                     FSLVYAFTENFLLPFSHDEVVYGKGSMLRKMSGDEWQRFANLRLLYGFMFGHPGKKLL
                     FMGDEFGQWSEWNHDASLEWHLLES"
BASE COUNT          235 a          268 c          305 g          270 t
ORIGIN      
        1 gcaaagagca actctttgga ctctcccatt tccgcttacg aattgcactt aggctcctgg
       61 cggcgcgtgc ctgaagaagg aaatcgctgg ctgacttatc gggagatggc gccactcctg
      121 gcggattacg tgcgggagac cggtttcact cacgtcgaat tgctgccagt gatggagcat
      181 cctttcgatg gctcctgggg atatcagacc actggttact ttgcgcctac cagcagattc
      241 ggcacgccgg acgatttcag gtatcttgtg gactgtcttc accaaaacgg aatcggcgtc
      301 atccttgatt gggttccggc ccacttcccg aaagatgaga cgggtctcgg ctatttcgat
      361 ggatctcatc tctacgaaca cggcgacccg aggcagggag aacacccgga ctggaacact
      421 tttgtgttca actacggtcg gaacgaagtc caaacttttc tcatcagcaa cgccctgttc
      481 tggttcgaca aatatcatgt ggacggtctg cgagtcgatg ccgtggcctc aatgctctat
      541 ctcgattatg gacggcgcga aggagagtgg ataccaaacc gttacggcgg aagagaaaac
      601 atagaagcga ttcactttct gagattgctg aacgagcatg tttatgccgc atttcccgat
      661 gtgatgatga tcgcggagga atcgaccgcc tggccgcagg tctcacgtcc cacttacgtt
      721 ggaggacttg gattcggtct caagtggaat atgggctgga tgcatgacgt gctgggctac
      781 atgtcgaaag acccattatt ccgcagctat gaccataaca agatcacgtt tagtttggtt
      841 tatgccttca ctgaaaactt ccttttgccg ttttcccatg atgaggtcgt ctacggtaag
      901 ggttccatgc tgcggaagat gtcaggcgat gagtggcaga ggttcgcgaa tctgcgcctt
      961 ctgtacgggt ttatgttcgg ccatcctggc aagaagctcc tgttcatggg cgatgagttc
     1021 ggccagtggt cggagtggaa tcacgatgcg agcctggagt ggcatctcct ggagagtt
//
LOCUS       DATMKH010000615         1094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1247467, whole genome shotgun sequence.
ACCESSION   DATMKH010000615 DATMKH010000000
VERSION     DATMKH010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1247467"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            28..>1094
                     /locus_tag="VNV63_07330"
     CDS             28..>1094
                     /locus_tag="VNV63_07330"
                     /inference="COORDINATES: protein motif:HMM:NF019135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HXC62470.1"
                     /translation="MLWLATGSGLFRLNPDTAQILHYVHDPANSSSLGSNQVRYTLED
                     RTGRFWVADGENLEEFDRERGRVLSRVRLSEGMRLVSFYEDHLGVFWISYITNGSGSG
                     LAVLDRSTNSLIPYSIYDRKSGKELPAGFMAAIEDNGQTLWLASFGAGLLKLDREHGV
                     FIRYRNYPENLESIADDRLMSLCGDREGNVWVGLLASEPNFFRTETAPFTPLRVIRSD
                     PRSYGEKIVNAVYEDRDHLLWMATAGALNRINRSSGESTSYQPPGQGLSSDVIAITED
                     RAGGLWIGSYGAGLSRFDRRTGRFKTYLHEADNPSSLSNSIVSRVLIDHSGTMWVATY
                     DGLDRFDVRNERFVVYKQDTK"
BASE COUNT          261 a          256 c          317 g          260 t
ORIGIN      
        1 ggccagatca gtcaggatcg gaccgggatg ctttggttgg caacaggaag tggcttgttt
       61 cggttgaacc cggatacggc tcagatcctc cattatgttc acgatccggc aaattcatcc
      121 agcctcggca gcaaccaggt cagatataca ttggaagata ggactggtcg attctgggta
      181 gctgacgggg agaatttgga ggagtttgat cgtgagcgtg gaagggttct ctcccgcgtc
      241 cgcctgagtg aaggtatgag gttagtttcg ttctatgaag atcatcttgg cgtcttttgg
      301 atttcgtaca tcacgaatgg cagtggaagt ggactagccg ttctggaccg atccacaaat
      361 agcctaattc cttactcaat ctatgacagg aagtctggca aagaattacc tgctggattc
      421 atggcggcaa tcgaagataa cggccaaact ctttggctag ccagtttcgg cgcggggcta
      481 ttaaagctgg acagagagca tggcgttttc atccggtatc ggaattatcc agaaaatctt
      541 gagagcatcg cagatgaccg tttgatgtcg ctgtgcgggg accgcgaagg aaatgtttgg
      601 gtgggcttgc tcgcgagcga gcccaacttt ttccgtactg aaacggcgcc cttcacgcct
      661 ctgcgtgtta ttcgaagcga tccccgcagt tacggtgaaa agatcgtaaa cgcggtgtac
      721 gaagaccgcg accacctttt gtggatggcg acagcaggag cgctaaatcg catcaatcga
      781 agctctggag aatccacttc ttaccaacct cccggccaag gactgtcgag cgatgtaatt
      841 gcgatcactg aagatcgggc cggtggtctc tggatcggaa gctacggcgc gggattaagc
      901 cggttcgatc ggaggaccgg gcgatttaag acctatcttc acgaagcaga caacccatcc
      961 agtttgagca acagcatcgt gagccgggtg ctgatcgatc attcgggcac catgtgggtc
     1021 gcaacctacg atggtcttga tcgatttgac gttcggaatg agaggttcgt tgtctacaag
     1081 caagacacta agag
//
LOCUS       DATMKH010000616         1012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3159731, whole genome shotgun sequence.
ACCESSION   DATMKH010000616 DATMKH010000000
VERSION     DATMKH010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3159731"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(206..514)
                     /locus_tag="VNV63_07335"
     CDS             complement(206..514)
                     /locus_tag="VNV63_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62471.1"
                     /translation="MRVEIGEGAWPIFKIEVSRVEIEDGARDVRSISDETLVAGVDLE
                     EKGEFDKEMRGVRVKDDEMIRGRRSKDSRRCTVRGLGWSLGDQTKAGVEAHERGGGTL
                     "
BASE COUNT          234 a          301 c          223 g          254 t
ORIGIN      
        1 gatctaagtg gatctcctag atccggagat aacgcacgga cctcactccc cttgtacgaa
       61 tagatagggt cgtctccggt agaaatatat gatagtgctt ttgccctctc ccctttgcgt
      121 gcccgcaaag ctcagaatag ttaggatccc gtgcgacata taaaagaact ggagcctgtc
      181 cttgagacag gcaggcaacg atcgcctaca gtgttccccc ccctctctcg tgtgcttcca
      241 ctccggcctt cgtctgatct cccagcgacc aaccgagccc gcgtaccgta catcgtcttg
      301 aatctttcga tcggcgccct cttatcatct catcatcttt gactcgtaca ccccgcatct
      361 ccttatcaaa ttctcctttt tcctcaagat ctacaccggc cacaagagtt tcatcactta
      421 tcgatctgac atcgcgcgcg ccgtcctcaa tctcgacgcg cgacacctcg atcttgaaga
      481 tcggccacgc tccttctccg atctcaaccc tcaacgcgtt cgtttatcct tcgggatcgc
      541 gggcccgtct ataaatctat taaatctatc atccttccat cgaactcacc gagcgctctt
      601 aagttccttg gaactttccc acactcacag aacgtacagt ttattcctgg gtttatggcc
      661 agtattgagc cctgatctat accagcctgt tgtccgattg aggagcacga agcattattt
      721 tcactaggta cgaccgtatg aaggagcaga tgcagttagt gaagagaaaa ggtaggtcgg
      781 acacgccgtc gggacatacg atacgccgcg atactcctct gtatgggatc gagcgcccag
      841 tcagctcctt gcgagattgg ttgggaacgg aaaatgaaga cgtatgcatt gcattttggg
      901 accggtagca tccacaaaac cggtcagcat cgcgcaaaaa gcgcgcctcc gcagccacgc
      961 gaaagtcgcg catgatgccc ttggtgtgag tgaatgatca tattcgacct at
//
LOCUS       DATMKH010000617         1696 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3476700, whole genome shotgun sequence.
ACCESSION   DATMKH010000617 DATMKH010000000
VERSION     DATMKH010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3476700"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            219..536
                     /locus_tag="VNV63_07340"
     CDS             219..536
                     /locus_tag="VNV63_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62472.1"
                     /translation="MSEEPQSDLLKGWKQIAEFLGQPISVAQRWATTGMPVTRQGRFV
                     VATPEELNNWLGRESGEPVHVVTNEADLSEELKRGLSHVRKEQRASIAPAQEPARKRM
                     RGK"
     gene            complement(605..1267)
                     /locus_tag="VNV63_07345"
     CDS             complement(605..1267)
                     /locus_tag="VNV63_07345"
                     /inference="COORDINATES: protein motif:HMM:NF018749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1349 domain-containing protein"
                     /protein_id="HXC62473.1"
                     /translation="MHIVRWVVLFASICLLVVTPAAAQSLPSIPTLPGKLEWLNAPRS
                     SRMDGKVFVISSGAKTDWFVDPFDGTVSKNAPILLFTPGSDYVLTARVTVKFATKWDA
                     GALMLWGDDHHWAKLSFELSPDRQPTLVTVVTRGLSDDCNSAHMQGDSVYLRIARSDK
                     TYMFYSSVDASHWEILRTFSLDTDLPIRAGFESQSPAGEGAIASFSSITYTPRRIENI
                     YK"
     gene            complement(1452..>1696)
                     /locus_tag="VNV63_07350"
     CDS             complement(1452..>1696)
                     /locus_tag="VNV63_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62474.1"
                     /translation="RLQARVFEGGLMEKEPEIEREESVTVNFKSETEPGKTYSGDKIP
                     DDALCGVCERKCSEHTVEMYKACARKQRELLFRAHK"
BASE COUNT          420 a          441 c          473 g          362 t
ORIGIN      
        1 ggaaccgatg taacgaggct tcctgcccgg ttttctgttt tcagggaagc gactctccga
       61 gctcttcttg cccaatttcg acaactcatc cgaggaacta tggcgctcac aaggcgaatt
      121 ctttccccga cgtaaaaacg caagattccc atctttcgcc ggtcccaaaa catcataatt
      181 catgcgcatt caaattgatc agggcaagga gcccgacagt gtctgaggaa ccacagtccg
      241 acctgttgaa ggggtggaaa caaatcgcgg aattcctggg acagccgatt tcggtcgcac
      301 aacgctgggc cacgacgggt atgccggtca cccggcaggg ccggtttgtg gttgcaactc
      361 cggaagaatt gaacaactgg ctgggaagag aatccgggga accggtacac gtggtgacaa
      421 acgaggcaga cctgtcggag gaattgaagc gaggactatc ccacgtgaga aaagaacagc
      481 gcgcttcgat agctccagct caggaaccag cacggaagcg gatgagggga aaataacttc
      541 ttattacact gatgatcagc ggtttcagta tcaaatctga agcggaccca tcgcgtatct
      601 tccgttactt gtagatgttc tcaatgcggc gcggagtgta ggtgatggag gagaacgatg
      661 cgatcgcgcc ttccccggcg ggcgactgcg attcgaagcc ggcgcggatg gggagatcgg
      721 tatcgaggct gaaggtacga aggatttccc agtggcttgc gtccacggat gagtaaaaca
      781 tgtaggtttt atcgctcctc gcgatcctca ggtacacgga atctccttgc atatgcgccg
      841 aattacagtc atccgagagc ccgcgcgtga cgactgtcac cagagtgggc tgacggtcag
      901 gagacaactc gaatgacagc ttcgcccaat ggtgatcatc accccacagc atcagtgctc
      961 ccgcatccca ttttgtcgcg aacttaacag tgactcgtgc ggttaagacg taatcggagc
     1021 ccggagtgaa gagcaggatc ggagcattct tggaaacggt tccatcgaag ggatcgacga
     1081 accagtcggt cttggctccg gaggaaatga caaagacctt tccatccatt cgcgatgacc
     1141 tgggcgcatt gagccactca agcttgcccg gaagagtcgg gatggagggc agcgattggg
     1201 cagcggcagg cgtcacgacg agcaaacaaa tcgatgcaaa aagcacgacc caccttacga
     1261 tgtgcacaga catgtttctc ctgttggatt caatggaaaa atactacgaa aggtcaggcg
     1321 gaaaaagccc tcgccatcat caaggaatac caggcgggga acgaacaggc gggatagaat
     1381 gtaatcacac aggcagaact ggcacagaag gcggcctctc gtccgaaggg ataagataag
     1441 atgccgcttt cttatttgtg cgccctaaag agcagctctc tctgcttccg tgcgcacgcc
     1501 ttgtacatct ccacggtatg ctctgagcat ttcctctcgc agactccgca aagtgcgtca
     1561 tcgggaattt tgtcgccgga ataggtcttg cctggttcgg tctcgctctt gaaattcacg
     1621 gttacggatt cttcgcgttc tatctcgggt tctttttcca tgagtccccc ttcaaaaaca
     1681 cgggcttgga gtcttg
//
LOCUS       DATMKH010000618         1885 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1249082, whole genome shotgun sequence.
ACCESSION   DATMKH010000618 DATMKH010000000
VERSION     DATMKH010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1249082"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="VNV63_07355"
     CDS             complement(<1..505)
                     /locus_tag="VNV63_07355"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HXC62475.1"
                     /translation="MKERQRDRVVGIALSGGAARCIGQIGVLETLEKAGIQIDAVAGT
                     SGGAFVGALYASGQVSIPEMRRLARGIKWRHVVRPNLSKKGIISSEKIYHYVRDVLND
                     MTFEDLKIPLAVVASDLRSGDKVVITEGSVARAVQASCSLPVVFTPTSLNGQVLIDGG
                     AASQLPVL"
     gene            complement(564..1322)
                     /locus_tag="VNV63_07360"
     CDS             complement(564..1322)
                     /locus_tag="VNV63_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPMC system MBL fold metallohydrolase"
                     /protein_id="HXC62476.1"
                     /translation="MMRLRILGSGTSTGVPTIGCNCLVCTSSDPRNIRTRASLWIEYE
                     DRHFLIDTSTDLRQQALINKIPRVDAVLFTHAHADHVLGLDELRVYNFYQKAAIPCYG
                     EKETLEKLRQMFSYIFKSEAVESFKPQLSLHEITSAFKLHGLEILPVPVRHGSLLVFG
                     YRFGDTAYVTDCNFIPEESKKMLKHLDLLILSAIRYQPHPTHFSLNEALEAIRELAPR
                     RAILTHLSHAFDFSKVNAELPKGIELAYDGMRIE"
     gene            1593..>1885
                     /locus_tag="VNV63_07365"
     CDS             1593..>1885
                     /locus_tag="VNV63_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXC62477.1"
                     /translation="MWSCKNFAFVLALLLIGGTGCSQPSDTIRIAVAGPMTGDQSKEG
                     TDLKNGVELAVAEWNEKGGLIGKKIELLIEDDQHDPKQAVAVANKLVNSGVVGV"
BASE COUNT          456 a          495 c          509 g          425 t
ORIGIN      
        1 ccagaaccgg caactggctt gcggccccgc cgtctatcag gacctgacca ttcaaagaag
       61 tcggtgtgaa gaccaccggc aggctgcagg atgcctggac cgcacgggcg accgagcctt
      121 cggtaataac gaccttgtca ccgcttcgga gatcgcttgc cactactgcc aacgggatct
      181 tgagatcctc aaaggtcata tcgtttaaaa catcccggac gtagtgatag atcttctcgc
      241 tcgagatgat ccccttcttg gagaggtttg gccggacgac atgccgccat tttatccctc
      301 gtgcgagtcg cctcatctcc gggatcgaga cctggcctga ggcatagagc gcgcccacaa
      361 aagcaccgcc gctcgtaccg gcaactgcgt ctatttgaat gcctgccttt tcaagcgtct
      421 ccagcacgcc gatctggccg atgcacctcg ctgctccgcc gctcagggcg atcccgacaa
      481 cccggtctct ctgtctttcc ttcatgattt tttacggctg tttcggggat tgatccgcct
      541 ggctgagcag acgggaccca ccatcactcg atcctcattc catcgtaggc gagctcgatt
      601 cccttcggca actcggcgtt gaccttggaa aaatcgaagg catgggagag atgggtcaga
      661 atagcgcgcc ggggcgccag ctcacggatc gcctcaagcg cttcatttaa actaaaatgt
      721 gtcggatgag gctgataccg gatcgcgctc aggatcagaa gatcgagatg cttgagcatc
      781 tttttggact cttccggaat aaaattacag tcggtgacat aagcagtatc gccaaaccga
      841 tagccgaaga ccaacagact tccgtgacgg accggcaccg gcagaatctc taaaccatgg
      901 agcttgaacg cgctggtgat ctcgtgcagc gagagttgcg gcttaaagct ttcaaccgcc
      961 tccgatttaa agatgtagga aaacatctgg cggagcttct cgagcgtctc cttctctccg
     1021 taacaaggga ttgctgcctt ctgatagaag ttatacacgc ggagctcgtc cagccccagg
     1081 acatgatcgg catgagcgtg cgtaaaaaga accgcatcca cccgtggaat tttgtttatc
     1141 agagcctgct gcctcagatc cgtcgaggta tcaatcaaaa agtggcggtc ttcatattcg
     1201 atccaaagcg aggcacgggt gcggatattc cgcggatcgg aagaggtaca gacaagacaa
     1261 ttgcaaccga tggtcggaac cccggtcgag gtcccgcttc caagaatcct taatcgcatc
     1321 attcgtacag ttccattggg atgttaagat cagagattgt aaaacagaca aagctcaagc
     1381 gcctttgact ctagctattg gtatcaaggt aacatgttta agccattttc gcaaccatgg
     1441 ttttttgctc tcgctagagg agatggaagc ttcgagcctt tgttataaag ttgggtagca
     1501 aggttcaaga aatcggttga caatccatcc tgttcttgat aaaagctggc ttcgagatga
     1561 agcagcgttt ttatttgtag gagaagaacg gaatgtggag ttgtaagaat tttgcctttg
     1621 tcctggctct cttactgatc ggaggaacgg gatgcagcca accgtccgat acgattcgca
     1681 tcgccgtggc cgggccgatg accggggatc agagcaagga gggcaccgac ctcaaaaacg
     1741 gcgtagagct ggctgtggcg gaatggaacg agaagggcgg actgatcggg aaaaagatcg
     1801 aactccttat cgaagatgat cagcacgacc ccaagcaggc tgtggctgtt gcgaacaaac
     1861 tggtcaactc cggagtcgtg ggagt
//
LOCUS       DATMKH010000619         1538 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3478766, whole genome shotgun sequence.
ACCESSION   DATMKH010000619 DATMKH010000000
VERSION     DATMKH010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3478766"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..427
                     /locus_tag="VNV63_07370"
     CDS             152..427
                     /locus_tag="VNV63_07370"
                     /inference="COORDINATES: protein motif:HMM:NF020295.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU N-terminal domain-containing protein"
                     /protein_id="HXC62478.1"
                     /translation="MADQIQVTAEPTPNPNSVKFSLNRVVTAEGSESYDNPQEAANSP
                     LAKRLFDVKGVNALFFLKNFVSVVKDSGSGWDEIVPQVKEILKDYFA"
     gene            454..861
                     /locus_tag="VNV63_07375"
     CDS             454..861
                     /locus_tag="VNV63_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62479.1"
                     /translation="MTIEEKWQAAKDKAAYAKRFPGMITTWEALAGKTFAQVLPLKKK
                     PFILLLFADRSFAFAPSLDLDPSDLVPCLEEARPYLEKIYPEDYRTLDDLAFKNRELQ
                     RRARLENILGAIQNNLAEIPELKNELKKFLERL"
     gene            879..>1538
                     /locus_tag="VNV63_07380"
     CDS             879..>1538
                     /locus_tag="VNV63_07380"
                     /inference="COORDINATES: protein motif:HMM:NF040244.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HXC62480.1"
                     /translation="MTQELVKPEKFFTEKFGITSQQLEKIMGQALGRPLDDADLYFEY
                     RVNESVSLEERLVKKAVRQVSQGVGVRAIAGEKTGYAYSDEITLKNIEISARTAKAIS
                     FQSGKTAVVQVGASRSHSHDLYPVKESVTDIPVEQKIELLHKIDALARQYDPRITKVM
                     ASIASEWKVILIATAGGVVVGDIQPLTRLQVSCIAEEKGDRQTGSFGGGGRVEYSYFL
                     EG"
BASE COUNT          368 a          362 c          444 g          364 t
ORIGIN      
        1 caagttcggt tgaggcccat cttgaggtcc tttctaaatc gaattagagg ggcccctccg
       61 ggaagaatga attgacacgc tgtgctgttt ttgtttatca tgagatgcgt ttcccttcag
      121 gacagaaagg ttttctagaa aggagttttt tatggcagac cagatccagg tgacggctga
      181 gcccacaccg aatcccaaca gtgtaaagtt cagtctcaat cgggtggtaa cggctgaggg
      241 gagcgaatcg tacgataatc cgcaagaggc tgccaactcg ccgttagcca aacggctctt
      301 tgacgtcaag ggtgtcaacg cgcttttttt ccttaagaat tttgtatcgg tggtaaagga
      361 ttccggaagc ggctgggatg aaatcgtccc gcaggtcaaa gagatcttaa aagattattt
      421 tgcctaatcc gatcccctat tagtttagtc attatgacaa tcgaagagaa gtggcaagcc
      481 gccaaggaca aggctgcgta cgccaagcgg tttcccggga tgatcacgac gtgggaggcc
      541 ttggccggca agacgttcgc ccaggtcttg ccccttaaga agaagccgtt tattcttctg
      601 ctttttgcgg atcgctcttt tgcttttgct ccgtctcttg atctggatcc ctccgatttg
      661 gttccatgcc tggaggaggc gagaccttac cttgagaaga tctatcctga ggactatcgg
      721 acgctggacg atctggcctt caagaatcga gagctccaac gccgcgcaag gctggagaat
      781 atcctggggg cgatacagaa caatctcgcc gagatcccgg agcttaagaa cgagctaaag
      841 aaatttctgg aaagacttta acaaaagtga ggtgattcgt gacacaggaa ctggtgaaac
      901 ccgaaaagtt tttcacagaa aagtttggga ttacttccca gcaactcgaa aagatcatgg
      961 gtcaggccct cggccggccg ttggatgatg cggatctcta ttttgaatat cgtgtgaacg
     1021 agtccgtttc gcttgaggaa aggctggtca aaaaggcggt gcgccaggtc agccagggag
     1081 tcggggtgcg ggcgattgcg ggcgagaaga ccggctatgc ttattcggac gagatcacgc
     1141 tcaagaatat cgagatatcc gccaggaccg ctaaggcaat ctcgttccag tccgggaaga
     1201 ccgctgttgt ccaggtgggc gcttccaggt ctcattcgca tgatctttat ccggtcaagg
     1261 agtctgtcac cgacatcccg gttgagcaga agatcgagct tctccacaag attgatgccc
     1321 tagccaggca gtatgatcct cgtataacaa aagtcatggc ctcgattgca agcgaatgga
     1381 aggtcattct tatcgccacg gccggcggtg tcgtcgtcgg agacattcaa ccgttgacgc
     1441 ggcttcaggt gagctgcatc gcggaggaga aaggcgaccg ccagaccggg agtttcgggg
     1501 ggggcggccg ggtggagtat agctatttct tggagggg
//
LOCUS       DATMKH010000620         1146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3162147, whole genome shotgun sequence.
ACCESSION   DATMKH010000620 DATMKH010000000
VERSION     DATMKH010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3162147"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..900)
                     /locus_tag="VNV63_07385"
     CDS             complement(<1..900)
                     /locus_tag="VNV63_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HXC62481.1"
                     /translation="MNSAELRQGFLDYFVSQGHTLVPSSLLIPAQDPTLLFTNAGMVQ
                     FKGVFLGEEKRSYRRAVSSQKCMRAGGKHNDLDNVGRTGRHHTFFEMLGNFSFGDYFK
                     AEAIAYGWELLIKRWGLPKDRLWITVYQDDDEAYDLWRKIGVPDDRIKRMGEKDNFWQ
                     MGDTGPCGPCSEILYDQGSQVWPPGHQCQGVGCDCDRYLEIWNLVFMQYTKDREGKLC
                     PLPKPSIDTGMGLERISAVSQGVTSNYDTDLFIPLFKTLSGMTDHDLPEIKQSMAGRV
                     VADHIRAITFLISDGALPSNEGRG"
     gene            complement(887..>1146)
                     /locus_tag="VNV63_07390"
     CDS             complement(887..>1146)
                     /locus_tag="VNV63_07390"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HXC62482.1"
                     /translation="IGRDRVALELKVKGIAKELIEETLVPLYNEMDEQTLAQEALKKK
                     QRTMKRSTTASDHRRLTDFLRRRGFSYEAIRKALKANHEFC"
BASE COUNT          251 a          343 c          284 g          268 t
ORIGIN      
        1 cccgcgccct tcatttgagg gcaaggcacc atcactgatc agaaacgtga tggcccggat
       61 gtgatccgca acaacgcgcc cggccatgct ctgcttgatc tcaggtaaat cgtgatccgt
      121 catcccgctc aaggttttaa aaaggggaat aaatagatct gtatcataat tgctggtgac
      181 gccttgagag accgcgctga tccgctccag acccatcccg gtgtcaatgc ttggcttcgg
      241 cagagggcat agttttccct ctcgatcctt tgtgtactgc ataaagacca ggttccagat
      301 ctcgaggtaa cgatcgcagt cacaaccgac accctgacat tgatgccccg gaggccagac
      361 ctgcgacccc tgatcataca ggatctcgga gcagggcccg caaggaccgg tatcgcccat
      421 ctgccaaaag ttgtctttct cccccatccg tttgatacga tcatccggga cgccgatctt
      481 ccgccagaga tcgtaggcct catcatcatc ctgatagacc gtaatccaaa gtcgatcctt
      541 cggcaggccc cagcgtttga tgagaagctc ccaaccgtag gcaatcgcct cggccttgaa
      601 atagtcccca aaggaaaaat tccccaacat ctcaaagaag gtgtggtggc gtcccgtccg
      661 gccgacattg tcgaggtcat tatgcttccc gccggcccgc atgcatttct gagaagagac
      721 cgcccgccga tacgaccgct tctcctcgcc taaaaagacg cccttgaact ggaccattcc
      781 ggcattcgtg aaaagcaacg tcgggtcctg ggccgggatc agtagcgagg atggcaccag
      841 cgtgtgcccc tggctcacga agtagtctaa gaaaccctgt cgtagttcag cagaattcat
      901 gattggcttt gagcgctttt cggattgctt catacgaaaa ccctctccgg cgcaggaaat
      961 ctgtgagcct gcggtgatcg gaggctgtcg ttgatcgctt catcgtgcgc tgttttttct
     1021 ttaaggcctc ttgtgccagg gtctgctcgt ccatctcatt gtaaagcggg acgagcgtct
     1081 cttcgatcag ttccttggca atccccttga cctttaattc cagagctacc cggtctcgac
     1141 caatct
//
LOCUS       DATMKH010000621         2268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1250302, whole genome shotgun sequence.
ACCESSION   DATMKH010000621 DATMKH010000000
VERSION     DATMKH010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1250302"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="VNV63_07395"
     CDS             complement(<1..388)
                     /locus_tag="VNV63_07395"
                     /inference="COORDINATES: protein motif:HMM:NF025774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEDS domain-containing protein"
                     /protein_id="HXC62483.1"
                     /translation="MTSPQINTLLIEAPVGRHFAQLHRDSESLVGSVTLFIETGLRRG
                     TSVVVVATDTNTEQFLERLRRSGLEPETYRKSGQLKLFNAESMLSRFMRGGMPDWVNF
                     RKTVGTALEEVQAFGQSATRACGEMVN"
     gene            complement(590..665)
                     /locus_tag="VNV63_07400"
     tRNA            complement(590..665)
                     /locus_tag="VNV63_07400"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(630..632),aa:Ala,seq:ggc)
     gene            complement(703..1563)
                     /locus_tag="VNV63_07405"
     CDS             complement(703..1563)
                     /locus_tag="VNV63_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62484.1"
                     /translation="MISPFTPHQPVFGPEKFFGRGGLVRSLVQRLRDSQSVLLYGGPR
                     IGKTSMLLQVRSQLKQTVFLDLASQADLAALWSGNHDSVILLDGCDQIAKQEGLLEAV
                     QKFIQGPVAPKAIVLTGGRAWREYVRDQRIFDLKLQPFPLAVYLDKEAKSFLKPYLAV
                     ADIAEMMRFTGNHPYLLQLLMAEWWRHRRQDFARLALGCGKELDDFFKDSLAQLGYGL
                     ERRLLDYLIEQGRPINPRQAAKDLGQADIKPAADMLCYLGLISRWIRDTEATLSAGCE
                     LFNDRYRKLP"
     gene            1646..1942
                     /locus_tag="VNV63_07410"
     CDS             1646..1942
                     /locus_tag="VNV63_07410"
                     /inference="COORDINATES: protein motif:HMM:NF014633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein"
                     /protein_id="HXC62485.1"
                     /translation="MRTMQPKEVKNGVMINVRVKPRARQFSIKQIEDQIVVEVKSPPV
                     KGKANDDVIRELSGFFQKPVQLLKGHKNKHKILLIQGITLPEFRSRLDSVESLS"
     gene            complement(1934..>2268)
                     /locus_tag="VNV63_07415"
     CDS             complement(1934..>2268)
                     /locus_tag="VNV63_07415"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HXC62486.1"
                     /translation="RTERLPNIVSFCLEYIEGEAIVMLLARHGIYVAAGSSCSSPELR
                     ASSVVLALGIPPQIAQGAVLMSVGKDTTQYEIDRVLEVFPTLVRKLRAMSPVYEEVIK
                     HGTTKLSR"
BASE COUNT          559 a          601 c          592 g          516 t
ORIGIN      
        1 cgttcaccat ttcgccacag gcccttgtgg cgctctgccc gaatgcctga acctcctcga
       61 gagcggttcc gactgtcttg cgaaagttca cccaatcggg catacccccg cgcataaacc
      121 ggctgagcat cgactcggca ttgaataatt taagctggcc cgatttgcga taggtttccg
      181 gctccagacc gcttcggcgc aggcgctcaa ggaactgttc ggtattggta tccgttgcga
      241 ctaccactac gctggttcca cgccgcaggc ccgtttcgat aaatagagtc acagatccta
      301 ctagactttc cgagtctctg tgtagttgag caaaatggcg gcctaccgga gcttcgatca
      361 ggagcgtgtt aatttgtggg gatgtcattt tttctatact cttgtcttag ttttctgctc
      421 gccgaaggaa atgccttgag caagaggtgt ttatttaata tgtcatcaaa aaatttagtg
      481 cgaactgatt ggttttagat cattagtgat gctgacgtta tcatatatag agaaattcat
      541 gtcaatcagg tgaatacctg attttggcag agtgacggta ggggcaaatt ggtggagctg
      601 agggggatcg aacccctgac ctcaagactg ccagccttgc gctctcccaa ctgagctaca
      661 gccccacgaa gaatgcatcc tatcatccgt cgccgagcct tgtcaaggca gctttcgata
      721 ccgatcattg aacagctcgc atccggccga aagtgtcgcc tccgtatccc gaatccatcg
      781 gctgatgaga ccgagatagc aaagcatgtc ggctgcgggc ttgatgtcgg cttgaccgag
      841 atccttggcc gcttggcgag gattgatcgg acggccttgc tcaatcagat aatccagcag
      901 gcgtcgttcc aagccataac ccagttgggc cagtgaatcc ttgaaaaagt catctaattc
      961 tttcccacaa cccaaagcca gacgggcgaa atcttgacgg cgatgcctcc accactcggc
     1021 catgaggagc tgaagcagat agggatggtt tccggtgaac cgcatcatct ccgcgatgtc
     1081 ggcaaccgcg aggtaaggtt tcagaaaact cttcgcttct ttatccagat aaaccgctaa
     1141 cggaaaaggc tgaagcttca aatcaaagat cctctggtct cttacatatt cccgccaggc
     1201 ccgacccccg gtaagcacga tcgccttcgg tgccaccgga ccctgaatga atttttggac
     1261 cgcctcaagg aggccctcct gtttggcgat ctgatcacag ccatccagga ggatcacgga
     1321 atcatggttt ccgctccaaa gagcagccag atcggcctgg ctcgcaagat ccaaaaagac
     1381 cgtctgcttc agttgcgatc gaacctgtaa gagcatagag gtcttaccga ttcgagggcc
     1441 cccatagagc aaaaccgact ggctatcgcg gaggcgttgt accaggcttc ggacaagccc
     1501 gccgcggcca aaaaattttt ccgggccaaa aacaggttga tgcggcgtaa agggcgaaat
     1561 catggcaggg gtttattgac ctcgtgaggt aaaaaagaat agcacaattg acgctgggtt
     1621 gcaattgagt ttcggctgat ttatcatgcg gacgatgcaa cccaaagagg tcaaaaacgg
     1681 cgtgatgatc aatgtccggg taaaacctcg tgcacggcag ttttcaataa aacaaatcga
     1741 agatcagatc gtcgtggagg tgaagagccc gcccgtgaag ggcaaagcca acgacgatgt
     1801 gatccgggaa ctatcgggct tctttcaaaa acctgtgcaa ctcctaaaag gccacaagaa
     1861 caaacacaag attcttctga ttcaagggat cacgcttcct gaatttcggt cgcgcctcga
     1921 tagcgtcgag tcattatctt gataattttg tcgtgccgtg tttgatgact tcttcgtaga
     1981 ccggggacat cgcacggagt ttgcggacaa gtgtgggaaa gacctctaag actcgatcta
     2041 tctcgtactg ggtcgtgtcc ttcccaacac tcattagcac agccccctgc gcgatctggg
     2101 gcgggatgcc gagcgccaag acgaccgagg aggcacggag ctcaggagag gaacagctcg
     2161 atccggccgc gacgtagatc ccatgtcggg ccaggagcat tacgatcgcc tcgccctcaa
     2221 tatactccag gcagaagctc acaatgttgg gaagccgttc ggtccggt
//
LOCUS       DATMKH010000622         1641 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2517183, whole genome shotgun sequence.
ACCESSION   DATMKH010000622 DATMKH010000000
VERSION     DATMKH010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2517183"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="VNV63_07420"
     CDS             <1..338
                     /locus_tag="VNV63_07420"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62487.1"
                     /translation="AKLELTVTPHVTSDNQIVMHVKTDKKDPNFNQEVQNIPPLTTRT
                     AETDLLVKNGQTVVIGGIYTRTQSTENLAVPWLSKIPVLGWLFKNESKVDNTTELLIF
                     ITPTIQTGG"
     gene            346..669
                     /gene="cutA"
                     /locus_tag="VNV63_07425"
     CDS             346..669
                     /gene="cutA"
                     /locus_tag="VNV63_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HXC62488.1"
                     /translation="MAQKQAVVIFVTTSSSAEAGKIGRVLVEEKLAACANIVPLVRSI
                     YRWQGKICDESEALMVLKTRSGQIQKIIKRVRSLHSYTVPEIIVLPISAGSKDYLRWI
                     FEVTR"
     gene            1052..>1641
                     /locus_tag="VNV63_07430"
     CDS             1052..>1641
                     /locus_tag="VNV63_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62489.1"
                     /translation="MEVKKLKESYTIMVLPNPTAKAHRFSISKKALKTILGLCSVSLV
                     FLIIFLVQYVQALGERWELASLRKETKTQRLQIQTFTVAVNDLKKEMNRLQELDAKLR
                     VITDIGPPAEKNQLLGMGGPEEIDLDSPLSLESRRPEEVLHKMEQNVSRLQAEAAAQE
                     LSFQELTEAMKDHRSLWAATPSIWPVKGWLTSGFGNR"
BASE COUNT          412 a          415 c          437 g          377 t
ORIGIN      
        1 aagccaagct ggagctcacg gtcacgccgc atgtcacctc ggataaccag atcgtgatgc
       61 atgtcaagac tgacaaaaaa gacccgaact tcaaccagga agttcagaac attccgcctc
      121 tcaccacacg aacggctgaa accgatctgt tggttaagaa tggacaaacg gtggtgatcg
      181 gcgggatcta tacccggact caatctaccg aaaacctcgc cgttccttgg ctatccaaga
      241 tcccggtcct gggctggtta tttaagaacg agtccaaggt ggataacacg acagagcttc
      301 tgatctttat cacgcccacg atccaaacgg gaggttagta ttgccgtggc tcaaaaacag
      361 gcggttgtga tctttgtgac gacctcttcc tccgccgagg cggggaagat cggacgcgtg
      421 ctcgtggaag aaaaactggc ggcatgcgcc aacatcgttc cgcttgtccg ctcgatttat
      481 cgttggcagg gaaagatctg tgatgagtct gaggccctga tggtattgaa gacccgatcg
      541 ggacagatcc agaaaattat aaagagagtg cggtccctgc atagctatac agttcctgag
      601 atcatcgtcc ttcccatctc ggctggatcg aaagattacc tccgctggat cttcgaagtc
      661 acccggtaac caattacccg ttcttggact aaagtggtta aacagaaaac tcggtcccgc
      721 aaaacggagc tcatggccat gtatcataaa ttgctccaca tgcggcgctt tttaatgaat
      781 atcatgccct tctggttaat gtgggaaagg agagatgccg caaagagccg ctgtgtgacg
      841 gctgccccta ggaggagttc cttgtcacgc tctcaccggg aattcatgag gattaataag
      901 gtgatccgtc atttgccaaa tcgacgaaat ctgctatact ttctaagtac gtccgtaata
      961 gccttgactt gatcttgagg gcatggtatc atccccctca ttgtcttgcc tgcacctgac
     1021 ctgaaggcag attatgatgg atggaggttc tatggaggtg aagaaattga aagagtccta
     1081 caccataatg gtgcttccga accccacggc aaaggcgcac cggtttagca ttagcaagaa
     1141 ggctctcaag accatcctgg gcctgtgttc agtatctttg gttttcctga tcattttctt
     1201 ggtccagtat gtccaggccc tgggggaacg gtgggaattg gcttcgctgc gcaaggagac
     1261 caagacccag agattgcaga tccaaacctt taccgtagcg gtcaatgatc tgaaaaagga
     1321 gatgaaccgg ttgcaggaac tggacgcgaa gcttcgcgtg atcaccgata tcggcccacc
     1381 tgcggagaag aatcagctcc ttggcatggg ggggccggaa gaaatcgatc tggacagccc
     1441 tctctcctta gagagccggc gaccggagga agtccttcat aagatggagc aaaacgtttc
     1501 ccgtcttcag gcggaggcgg cggcacagga gctttccttc caagaattga ccgaggcgat
     1561 gaaagaccat cgttcccttt gggcggcaac cccttccatc tggccggtga agggatggtt
     1621 aacctctggt ttcgggaatc g
//
LOCUS       DATMKH010000623         1887 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_931355,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000623 DATMKH010000000
VERSION     DATMKH010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_931355"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1124
                     /locus_tag="VNV63_07435"
     CDS             <1..1124
                     /locus_tag="VNV63_07435"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXC62490.1"
                     /translation="LMRASKLESIGVLAGGIAHDFNNILTAIIGNLALAKMALGPENK
                     VIPRLVESEKVVLRAQSLSQQLLAFSKGGKPVKQVMSVGKLLKESVVFALQGSNVHSE
                     FSIPDDLWPIEADQGQITQVVHNIVLNAQQAMPTGGNIQIKAANFVVGSGAGLHLAAG
                     DYVKIRFNDQGTGISQENLAKIFDPFFTTKPKGSGLGLASAYWIIQRHHGYIPIDSKL
                     GAGTTVSVYLPRSERETPAEEPAEEALITGQGRILFMDDEEAIREFASEVLSQLGYEV
                     ECAQNGLEAIELFRKARESRRHFAAAILDLTVRSGMGGKETGRRLREMDPGVKTIVSS
                     GYSNDAVLADFREHGFSARMTKPYTAENLSRILAEVIKI"
     gene            complement(1323..>1887)
                     /locus_tag="VNV63_07440"
     CDS             complement(1323..>1887)
                     /locus_tag="VNV63_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015718480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HXC62491.1"
                     /translation="FSKSTLRLDNFSNHFSEGIGTVKLTGEFMGTGNTVISGNFRPEG
                     KSSDFDLNIKIERTQMRSMNDLLRAYGNFDVTAGQFSLYSQLAVRNGEVKGYIKPLFK
                     DMKVYDKRQDKEKSAFHKLYERLVGGVAKLLKNTPTDEVATKTEVSGPLENPKFNTGE
                     TVVNLIKNSFFKAILPGFEKEVTQKKT"
BASE COUNT          436 a          492 c          495 g          464 t
ORIGIN      
        1 aattgatgag agcgagtaaa ctggaatcga tcggggtgct tgcaggcggc attgcccatg
       61 attttaacaa catcctcaca gcgattattg gcaacttggc gctcgccaag atggcgctcg
      121 gccccgagaa caaagttatc ccacgcctcg ttgagtccga aaaagtagta ttgcgcgcgc
      181 aatcgttatc tcagcaactc ctcgcgtttt caaagggagg caaaccggtc aaacaggtca
      241 tgtctgtcgg aaagctcttg aaggaatcgg ttgtgtttgc tctccagggc tcgaatgtcc
      301 actctgagtt ctcaatccca gacgacctgt ggccgatcga agcggatcaa ggccagatca
      361 cccaggtagt ccataatatt gtgctcaatg cccagcaggc gatgcccacc gggggaaata
      421 tccagatcaa ggcggccaat tttgtcgtgg gatcaggggc tggtttgcat cttgcagccg
      481 gcgactacgt caagatccgg ttcaacgatc aagggacagg gatttctcaa gaaaatctgg
      541 caaagatctt tgaccccttc ttcacaacca aaccgaaggg aagcggattg ggcctagcct
      601 ctgcgtattg gatcattcag cggcaccacg gatatatccc catcgattcg aaacttgggg
      661 caggaactac cgtttcggtc tacctcccca gatctgaaag ggaaactcct gccgaggaac
      721 ctgccgagga agccttgatc acaggccaag gacgaatact attcatggat gatgaggagg
      781 caattaggga gtttgcttct gaagttttga gtcaactcgg ttatgaggta gagtgcgctc
      841 agaatggctt ggaggccatt gagctcttcc gcaaggcccg ggaatctcgc cgtcacttcg
      901 ccgcggcaat cctggatttg acggtccgtt ccggcatggg cgggaaggaa acagggagac
      961 ggcttcgcga gatggatccg ggagtgaaga caatcgtctc cagcggctat tccaatgatg
     1021 ccgttcttgc ggatttcagg gagcacggtt tttcagcacg gatgaccaag ccgtacaccg
     1081 ctgaaaacct gagccggatt ctggctgagg tcattaaaat ctagaaatca gcttagatat
     1141 aaattgcggc gcaacgccct ccgctcctca attgcatcgg tcttgccgct ctctcggcaa
     1201 caatttcctc cactgtcggc gaatgaccct cattcagaaa ctcgcacacg aagcgccttg
     1261 tcgctaaacc gatattgggg atctgtagaa tttcccgttc ccaaagagaa acggtatcgg
     1321 gtttacgtct tcttttgcgt tacttctttt tcaaagcccg gcaagatcgc tttgaagaac
     1381 gaatttttaa tcaaattgac gacggtctcc cccgtgttga atttggggtt ctccaagggg
     1441 cccgagactt ccgtcttagt tgcgacttcg tccgtcggtg tgtttttaag cagtttggcc
     1501 actcccccaa ccaaacgttc gtagagcttg tgaaacgcgc ttttctcttt atcctgccgt
     1561 ttgtcgtaca ccttcatgtc cttgaagagt ggtttgatgt agccctttac ctcgccgttc
     1621 ctgacggcca gctgggaata cagcgagaac tgcccggcgg tcacatcgaa gttgccgtag
     1681 gcccggagca ggtcgttcat cgaccgcatc tgagtccgct cgatcttgat gttcagatcg
     1741 aaatcggatg actttccctc cggccggaag ttgccgctga tcacggtatt gccggtaccc
     1801 ataaattcgc ctgtgagctt gacggttccg ataccttcgc taaagtgatt gctgaaatta
     1861 tcaaggcgga gcgtgctttt gctaaaa
//
LOCUS       DATMKH010000624         4860 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2857711, whole genome shotgun sequence.
ACCESSION   DATMKH010000624 DATMKH010000000
VERSION     DATMKH010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4860
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2857711"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="VNV63_07445"
     CDS             complement(<1..452)
                     /locus_tag="VNV63_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005997460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding-motility protein MglA"
                     /protein_id="HXC62492.1"
                     /translation="MSFINYSSREINCKIVYYGPGLGGKTTNLIYIYKKTSPDSKGKM
                     ISLATETERTLFFDFLPLSLGNIRGFKVRFHLYTVPGQVFYDASRKLILRGVDGVVFV
                     ADSQVERMEANIESIDNLRKNLQDQGFNLDAISYTIQYNKRDLPNVVP"
     gene            complement(501..995)
                     /locus_tag="VNV63_07450"
     CDS             complement(501..995)
                     /locus_tag="VNV63_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HXC62493.1"
                     /translation="MPDSSLVMYEEESRLINAELSKLHQQTNAQATFLVDKNGQLIAS
                     IGENKNLDTTSLASLTAGNIAATGGMAKLLGEKEFSILFHEGEKDNIHISLIGQRVIL
                     VVIFDHRSSLGLVRLRVKKSVEILAQIFEQLAQKAETAKVSKPTSKSPFAEITEEDIE
                     KLFG"
     gene            complement(1115..1405)
                     /locus_tag="VNV63_07455"
     CDS             complement(1115..1405)
                     /locus_tag="VNV63_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62494.1"
                     /translation="MNEEIQQSVLEVVRAIANLNRAFAKRADDLQKLGKADELQQWTK
                     AAQAMLDSGNIYLAWAKHYALSAGAKINSEESEESDFLDEGTVWTSEQNPGP"
     gene            complement(1439..2188)
                     /locus_tag="VNV63_07460"
     CDS             complement(1439..2188)
                     /locus_tag="VNV63_07460"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HXC62495.1"
                     /translation="MKVFQAEQHRAFKAEEMVQILKLSAPKDPQKQLQAMEQIGSELQ
                     KLIEAGRITEVASGLFKARLFFDKEEFIEHALMGEMGNTSYRFQSPIFRLNIGVLSVY
                     FIRYRKQGDWIATIRDTTIGKDYCLIRRLRDGTYTIGSRPAVAGEDHHLQIEGRYISK
                     KHLTLTVSGDKINIEDQKTPNGTRIDFLTKEGLVRYEQAAGDFLKGTDPIGYKEAVKR
                     GRFVLERLLQHHQNFETTFFNAVVDSLLLAS"
     gene            complement(2364..2651)
                     /locus_tag="VNV63_07465"
     CDS             complement(2364..2651)
                     /locus_tag="VNV63_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017952298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster-binding protein"
                     /protein_id="HXC62496.1"
                     /translation="MYNVAFVENDKCIAQKGCRLCIMYCPEADCIKLDTREMKAYVVV
                     ERCKGCELCVVVCDAAKHNAIIMAPVDGATGEIMLDKDKAEAAALGQAYQG"
     gene            complement(2672..3355)
                     /locus_tag="VNV63_07470"
     CDS             complement(2672..3355)
                     /locus_tag="VNV63_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HXC62497.1"
                     /translation="MKKRINIRMSGLGGQGVVTSAHVMAMAANKDGKFSISNPFFGAE
                     KRMAPAESYCRIGEERIYDRGELVYPDIIVVYHPQVITLQKSYTAPFYSGIKKGGLII
                     INSNSALLSDEDKKYLADLNVSVYTFDATALALEVAGTELASNMALIGAMAGISKAVT
                     LQALDKALQDRFGKVYVASGGTATLDEAIKKKFAKKEQLLQKNMDTIRKSYELAVAWA
                     ESNQYAMAV"
     gene            complement(3392..4288)
                     /locus_tag="VNV63_07475"
     CDS             complement(3392..4288)
                     /locus_tag="VNV63_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HXC62498.1"
                     /translation="MSKEKIQICAEFYDIMPPEYRDLVEQATYGNEGRGWKDIGNSKE
                     LIEEHSLCAGCPESIAFRYILASLPNPEDTVMVGSTGCTSLVFPHVAVHNIHSLFGNQ
                     NAVASGLKRALTVRFPGRIKDVVVLAGDGATVDIGLDMTLQSWFRQEKFTTICFDNEL
                     YANTGGQESGLMRKGFVAKMAPQGKKFDKVRLPEIARESGCAYVACLTVSKPNLVERV
                     LRNAIYLAREVGPTYVQLYTPCILEIGKQSMEGLQEMRDSEGKEGRFALKEFITDEAK
                     QYLADLEAKKKQNAAPAAALQS"
     gene            complement(4322..>4860)
                     /locus_tag="VNV63_07480"
     CDS             complement(4322..>4860)
                     /locus_tag="VNV63_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HXC62499.1"
                     /translation="VCMDMECPPARMMRDPFVMKSNYISYMTHASWQEETRAACERSR
                     KHTIPMIGGLIETENVDADILIVASGTAVSQGREAIRLMEDEGMRVGLVKIKSLRPFP
                     YHELREATKNAKHIFVPEFNVIGWLAQELKATIPDNHRVVAGPHVAGGMTMPAEVIVE
                     EIKKSLGKHVTTLAGRGS"
BASE COUNT         1107 a         1318 c         1166 g         1269 t
ORIGIN      
        1 atcgggacaa cattgggtaa gtcccgctta ttgtactgaa tcgtgtaaga gatcgcgtcg
       61 aggttgaacc cctgatcctg aaggttcttg cggagattgt cgatgctttc gatgtttgcc
      121 tccatccgct ccacctgtga atcggccacg aaaacaacac cgtccacacc gcgaaggata
      181 agcttccggc tggcgtcata gaagacctgc cccggcaccg tatagagatg aaaacgaacc
      241 ttaaaccctc ggatatttcc caaggaaagc ggaagaaaat cgaaaaatag ggttctctcg
      301 gtctccgtgg ccagcgagat catcttgcct ttgctatcgg ggctggtctt tttataaata
      361 taaatgaggt tggtggtctt acccccaaga ccgggaccat agtagacaat cttacagtta
      421 atttcacgtg aagaataatt gataaacgac atatggatgc gtgccttctg tctcccttaa
      481 ggttctgcca gccagtttcg ctagccgaat aatttctcga tatcctcctc tgtaatttca
      541 gcaaatgggg attttgaagt cggtttgctc acctttgcag tctcggcctt ttgtgccaac
      601 tgctcaaaaa tctgagcaag gatctccacg cttttcttaa cccgaagacg aacaagcccg
      661 agggaagacc gatgatcgaa gatcacaacg agaatcactc gttgaccgat caaggagatg
      721 tggatgttat ctttctctcc ttcatggaac agaatggaaa actccttttc cccgagaagt
      781 ttcgccatgc cgcctgtggc tgcgatgttg cccgctgtga gggaggccag ggaggtggtg
      841 tccaaatttt tattttctcc aatgctggcg atcagctgtc cattcttgtc caccaggaag
      901 gttgcctggg cattcgtctg ttgatgcaat ttcgagagct cggcattgat cagccgcgac
      961 tcttcttcat acatgactag gctggaatca ggcattggaa ccttccaatc agattttaaa
     1021 taaatcttta aaattataaa ggaaatttct gaaggttgca agcattcaat gggttgggaa
     1081 gactatgaag gcaaagagca acaaaaagga tgatctacgg gcccgggttt tgctctgagg
     1141 tccagacggt tccttcatcg agaaagtcgc tttcttctga ttcttctgag tttattttgg
     1201 cgcctgcgga gagggcatag tgcttggccc aggccaggta gatgtttccg ctgtccagca
     1261 tggcctgagc ggctttggtc cactgttgca actcgtccgc ctttcccagc ttttgaaggt
     1321 cgtccgcacg ctttgcaaac gcccggttga gattagctat ggcccggacg acctccagaa
     1381 cggattgctg aatctcttca ttcataccgg tttccttcta cgccttccgg tctcctggtc
     1441 agcttgcaag caaaagcgaa tcaacaacag cattaaagaa agtagtctca aagttttgat
     1501 gatgctgaag gagccgttcc aggacgaacc gacctcgttt aacggcctct ttatagccta
     1561 taggatccgt gccctttaaa aaatcccccg cggcttgttc gtaccggacc aggccttcct
     1621 tcgtgaggaa gtcgatgcgc gtgccatttg gcgttttctg gtcttcaatg ttgatcttat
     1681 caccggacac cgtgagggtc agatgctttt ttgaaatata ccttccttcg atctgcaagt
     1741 gatggtcctc acctgccaca gcgggccgac ttccgatcgt ataggtgccg tcccgaaggc
     1801 ggcggataag acagtaatct tttccgatag tcgtgtcccg gatcgtggcg atccaatccc
     1861 cttgttttct gtacctgata aagtacacgg aaagaacgcc gatgttcagc cgaaagatag
     1921 gcgattgaaa acgataggag gtattgccca tctctcccat caaggcatgc tcgatgaact
     1981 cttctttatc aaaaaagagc cgtgccttga agagaccgga agcaacctct gtaatccggc
     2041 ctgcctcaat caacttttgc agctctgaac caatttgctc catagcttgc aattgtttct
     2101 gcggatcttt tggggcggaa agctttagga tctgaaccat ctcctctgct ttgaaggcgc
     2161 gatgctgttc cgcttgaaaa accttcaaga tcgggtcctt cagattggct atggtcgtac
     2221 ctggtgatgg tatcggagga accttatttt ctttaacctc tgaacttaaa gatggctcca
     2281 tagtatctcc agctttgaaa cgaacaaaaa acccccaccg ctgtgccgca tgggggaact
     2341 tttgatcgat aataaatcct gaactatcct tgataggcct ggcccaacgc ggccgcctct
     2401 gctttgtcct tatccagcat gatctcgccg gtggctccat cgacgggggc catgataatg
     2461 gcattatgtt tggctgcatc acagaccacc acacagagct cgcagccttt gcaccgctca
     2521 acaaccacat aggccttcat ttcccgggtg tccagcttaa tgcagtcggc ctcaggacag
     2581 tacatgatgc acagcctgca gcctttctga gctatacatt tgtcattttc aacgaatgcc
     2641 acattgtaca ttcagcttgg ttcctttccc gttaaaccgc catcgcatac tgattgcttt
     2701 cggcccaggc gaccgcaagc tcataggatt tccggatcgt gtccatattc ttctggagca
     2761 actgctcctt ctttgcgaac tttttcttaa tcgcctcatc caaggtggcc gttcctcccg
     2821 aggccacata gaccttgccg aatcggtctt gaagggcctt atccaatgct tgcagcgtca
     2881 cggctttgga aataccagcc attgccccga tcagcgccat gttcgaagcc agctcggtgc
     2941 cagcaacctc taaagccaga gcggttgcgt caaaggtgta gaccgatacg ttcagatcag
     3001 ccagatactt cttgtcctca tcggacagga gcgctgaatt ggagttgata ataatgagac
     3061 cgcccttctt aatacccgaa tagaagggtg cggtgtagct cttctgtaat gtgatcacct
     3121 gcggatggta caccacgatg atgtccgggt acacaagctc cccgcgatcg tagatacgct
     3181 cttcgccaat ccggcagtaa ctttccgccg gagccattcg tttctcggca ccgaagaacg
     3241 ggttggagat tgaaaacttc ccatctttgt tggcagccat ggccatcaca tgggcggaag
     3301 tcacgactcc ctgaccacct agcccggaca ttctaatgtt gatacgtttc ttcatggtct
     3361 ctctccttaa gtcaattcca gcaagatgcc tttaactctg taacgccgca gccggagcgg
     3421 cattctgctt tttcttggcc tctagatcgg ccaggtactg cttggcttcg tcagtaataa
     3481 actcttttaa ggcaaaacgt ccctccttgc cttccgaatc tctcatctct tgcaggcctt
     3541 ccatgctctg ttttccgatt tcaaggatgc aaggggtata gagctggaca taggtcggac
     3601 ccacttcccg agcgaggtag atcgcattgc gtaacacgcg ctctaccaga ttgggcttgc
     3661 tcaccgtgag gcaggcgaca taagcgcaac ccgattcccg cgcgatctcc ggcagcctca
     3721 ccttgtcaaa tttcttgcct tgcggtgcca tcttggccac aaatcctttg cgcatcagcc
     3781 cggattcttg accgcccgtg ttcgcataaa gctcattgtc aaaacagatc gtagtaaact
     3841 tctcctgtcg gaaccaggac tgcagtgtca tgtcaagacc aatgtctacc gtggctccat
     3901 ccccggcgag tacgacaacg tccttgatac gacccgggaa cctgacggtg agagcacgtt
     3961 tgaggccgct tgcgactgca ttctgattcc caaacaatga gtgaatgttg tgcaccgcta
     4021 cgtgggggaa gactaagctg gtgcagccgg ttgagcccac catcacggtg tcttccggat
     4081 taggaagcga ggccaggata taccggaagg caatggattc cgggcatccc gcacagagtg
     4141 aatgctcttc gatgagctcc ttgctgtttc cgatgtcctt ccatccacgc ccctcgttcc
     4201 cgtaagtggc ctgctccaca aggtcgcgat actcgggagg catgatgtca taaaactcgg
     4261 cacaaatctg aattttctct ttgctcatga tgtttctcct tttaatttga ccattgaaag
     4321 atcagcttcc tcgtccggcc aaggtcgtga catgcttacc cagggacttt ttgatctcct
     4381 ccacgatgac ttccgccggc atcgtcatgc cgcccgccac atgaggtccg gcaaccactc
     4441 gatggttgtc gggaatggtg gctttgagtt cctgggccag ccagccgatg acgttgaact
     4501 ccggaacaaa gatgtgcttc gcattttttg tggcttcccg aagctcatga tatgggaagg
     4561 gccgcagcga cttgatttta acgagaccaa ccctcattcc ttcatcctcc atcagccgaa
     4621 tggcctcacg cccttgcgat accgcggtgc cggatgccac aatcaggata tccgcgtcca
     4681 cattctcggt ctcgatcagc ccgccgatca tcggaatggt gtgttttcga gagcgttcgc
     4741 aggctgcccg agtttcttcc tgccaggaag catgggtcat atagctaatg tagttcgatt
     4801 tcatgacaaa aggatcacgc atcatccgag ccggaggaca ctccatgtcc atgcagacga
//
LOCUS       DATMKH010000625         1936 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1880259, whole genome shotgun sequence.
ACCESSION   DATMKH010000625 DATMKH010000000
VERSION     DATMKH010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1880259"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="VNV63_07485"
     CDS             <1..531
                     /locus_tag="VNV63_07485"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HXC62500.1"
                     /translation="GVVVVGVRQYVRTPVGRLQWAGLKLKLPVFGPIFLKTALSRFSR
                     VYGTLTRSGLPILETLDIVSKTVGNVVIAHVVDNIRDSARQGRGVVQPMKLSRIFPPT
                     VIQMVAVGEETGKMEEMLMKVSNYYDRDVEYAIRNLSTTLEPLLLLVIGSMVLFLALA
                     IFLPWWNMIRVFKGGG"
     gene            536..>1936
                     /locus_tag="VNV63_07490"
     CDS             536..>1936
                     /locus_tag="VNV63_07490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXC62501.1"
                     /translation="MLEGFVKRRVQISLYVFIPLFFALFAFGPAYIVVLIFSSYPSFS
                     DPAWVQGSVRHARLTLIIGIVTAGVAGLLIAYALLQPIKKLKEGLQAISLGDLTTRLA
                     LEASREFEHLGRDFNLMVTALNRYLLGVIGGVITVNREGKILSLNPIAELILGYRTEE
                     VAGQFIRDLKVAGLATDMLLDLIQETLTRQKPVATQELPLIVQEGQPISLSVSTSPLR
                     DEQGIVVGVVIQFKDLVRLRQVQDQMRRTDRLASLGMLAAGVAHEVRNPLGSIKGLVQ
                     LLSEDLQDAQKKRYAEIIIQEVDRLNRVVQDLLKFSQFSGSELKKARLNDLVRQAAML
                     AGFEKMKKEITLREEYDDQIPEMEVDGERLVQAFLNILLNAIQAIPESGEVWVKTAIT
                     GAGREPPLLAIIEVGNTRSVIPPNALKKIFDPFYTTKEQGTGLGLTIAHQIITAHGGE
                     IEVRSGNDKTVFTIGLP"
BASE COUNT          413 a          495 c          568 g          460 t
ORIGIN      
        1 ggcgtggttg tggttggggt ccgtcaatat gtccgtacgc cggtcggacg gcttcaatgg
       61 gccggtttga aactaaaact gccggtgttc gggccgattt ttcttaagac cgctctctcg
      121 cgatttagcc gggtctacgg gactctcaca cgcagcggct tgccgatcct cgaaaccctc
      181 gatattgtct ctaagaccgt gggcaatgtg gtgattgcgc acgtggtgga taacatccgg
      241 gacagcgcac gacagggacg aggggtggtg caaccgatga agctgagccg tatcttcccc
      301 ccgaccgtaa tccagatggt ggcggttgga gaagagaccg ggaagatgga agagatgctg
      361 atgaaagtgt caaactatta cgatcgcgat gtggaatatg cgatacgaaa cctctccacg
      421 actttagagc cgctgctgct gctggtcatc ggcagcatgg tcctgttcct ggcccttgcg
      481 atcttccttc cctggtggaa catgattagg gttttcaagg gcggaggtta aaggtttgct
      541 tgaggggttt gtcaagcgcc gtgtccagat cagcctttac gttttcatcc cgttgttctt
      601 tgcactgttc gcctttggcc cggcctacat cgtcgtcctc atcttttcca gctatccatc
      661 attttctgat cctgcctggg ttcaaggatc tgtccgtcac gctcgcctca cactgatcat
      721 tggaattgtc actgccggag tggccggcct gctgatcgcc tatgctcttc ttcagccgat
      781 taaaaagctt aaagaagggc ttcaagccat ctcattgggc gatctgacca cacggttggc
      841 cctggaagcc agccgggagt ttgagcatct gggccgtgat tttaatctca tggtcacggc
      901 cctcaatcgt tatctcctgg gggtgatcgg cggcgtgatt acggtgaacc gggaggggaa
      961 gatcctgtcg ctaaacccga tcgccgaact catactggga tatcgtacgg aagaagtcgc
     1021 agggcagttt atacgtgatc tcaaagtagc cggacttgcc accgacatgc tgttggatct
     1081 cattcaggag acgcttacac ggcagaaacc ggtagcgact caggagctgc ccctgattgt
     1141 gcaagagggt cagccgatca gtctttctgt cagtacctct cccctgcggg atgagcaagg
     1201 catcgtggtc ggcgtggtga ttcagttcaa agacctggtg aggctccggc aggttcagga
     1261 ccagatgcga cggacggatc gcctcgcctc gctggggatg cttgccgccg gcgtggcgca
     1321 tgaagtccgg aaccccttgg gttccatcaa aggcctggtt caacttttat ccgaggatct
     1381 tcaggatgcg cagaagaagc gttatgccga gatcattatt caggaggtgg accgtctcaa
     1441 tcgggtggtg caagatcttt taaagttttc ccagttcagc ggctcggaac tcaagaaggc
     1501 ccggctcaat gacctggttc gacaggccgc gatgctggcc ggttttgaga agatgaagaa
     1561 agagatcacg ttgagagaag aatacgacga tcagattcct gagatggagg tggatggaga
     1621 acggctggtt caggcttttt tgaatatact gctcaacgcg atccaggcga ttcctgaatc
     1681 gggcgaggtt tgggtgaaga cagcgatcac cggggcgggt cgcgaaccgc ctctgctcgc
     1741 cattatcgag gtcggcaaca cacgctcggt gattcctccc aacgccttaa agaaaatttt
     1801 tgatccgttt tacaccacca aggagcaggg aaccggcctc ggtctgacaa tcgcacatca
     1861 gatcatcacg gctcatgggg gcgagattga ggtcaggagc ggcaatgaca aaaccgtttt
     1921 cacaatcgga ctgccg
//
LOCUS       DATMKH010000626         1684 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_291999,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000626 DATMKH010000000
VERSION     DATMKH010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291999"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..172
                     /locus_tag="VNV63_07495"
     CDS             <1..172
                     /locus_tag="VNV63_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GMP synthase (glutamine-hydrolyzing)"
                     /protein_id="HXC62502.1"
                     /translation="IAIRAVTSQDGMTADWGRLPHEVLEAISNRIINEVKGINRVVYD
                     VSSKPPSTIEWE"
     gene            172..1083
                     /locus_tag="VNV63_07500"
     CDS             172..1083
                     /locus_tag="VNV63_07500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HXC62503.1"
                     /translation="MQQRLQKIISQAGITSRRKAEELILQGRVEVNGKVVRTLGSKAD
                     PEKDHVKLNGRLLQPNQPKVYMMLNKPKAYVTTLNDPEGRPTVMELLKGVHQRVYPVG
                     RLDFDAEGLLLFTNDGDLAEHLMHPRYEIPRIYLVKVKGVLEDQEIERLEAGVLLLDG
                     MTAPCRIKKVRKTAENSWVELTMREGRNRQVKRMMEHAGHPVLKLKRIRFAGLELADL
                     EVGEYRFLTENEIKQLKKTGQVTRPSAPRKIPSRPTQNRSVASTSSARNRFVASARPA
                     RSRSVASSRPVRQAHRAGRRTRTRQAR"
     gene            1095..>1684
                     /locus_tag="VNV63_07505"
     CDS             1095..>1684
                     /locus_tag="VNV63_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HXC62504.1"
                     /translation="MMKRTHTCSELRKQHIGQSAVLLGWVHGRRDHGGLIFIDLRDRE
                     GITQVVFHPEPNRSVHELAHQLRSEYVIGVSGSVHERPSGTANPKLATGEIELIADKL
                     EILNPAKTPPFQIEDESAVAEPIKLKYRYLDLRRPAMQKNFRLRHQLNKTIRSFLDKK
                     GFLEIETPFLTKSTPEGARDYLVPSRINPGRFYALP"
BASE COUNT          426 a          446 c          461 g          351 t
ORIGIN      
        1 gattgcgatt cgtgccgtga caagccaaga tggaatgacg gcggattggg gacgattgcc
       61 gcatgaggta ttagaggcaa tctcaaatcg catcatcaac gaggtgaaag gcatcaaccg
      121 ggtagtctac gatgtctctt ccaagcctcc gagcaccatc gagtgggagt aatgcaacag
      181 cggcttcaaa aaattatttc acaggcgggg atcacttccc gacgaaaagc ggaggaattg
      241 attcttcaag gccgggtgga ggtgaatgga aaagttgttc gaaccctcgg ttccaaggcc
      301 gatccggaga aggaccatgt taaattaaac ggccgcttgc tccagccgaa tcaaccgaag
      361 gtctatatga tgctcaacaa accgaaagct tatgttacga cgctcaatga cccggagggg
      421 agaccgaccg tcatggagtt attgaaggga gtacaccagc gtgtgtaccc ggtcggccgc
      481 ctggattttg atgccgaggg cctcctgctt ttcaccaatg atggagacct ggccgaacat
      541 ttaatgcatc cccgttatga aattccaaga atttacctgg tcaaagtaaa gggtgttttg
      601 gaagatcaag agatcgaacg tttggaagcc ggcgtactct tattggacgg catgaccgct
      661 ccgtgccgaa ttaagaaggt gagaaagacg gcggagaatt cctgggttga actgacgatg
      721 cgcgaaggac gaaaccggca ggtcaaacgc atgatggagc acgcaggcca tccggtcctc
      781 aagctcaaac gaatccggtt tgcagggctt gagctcgccg atctagaagt aggcgagtac
      841 cgctttctga ccgaaaatga gattaagcag cttaagaaaa ccggccaggt tacgcgtccg
      901 tctgctcctc ggaaaatccc gtcccggcct acccaaaacc gatctgttgc atctaccagc
      961 tctgctcgaa accgctttgt tgcatctgcc cggcctgctc gaagccgatc tgttgcatct
     1021 tcccggcctg tcagacaagc ccatcgggca ggcaggcgga caagaacgcg gcaggctaga
     1081 tagggcgaat gctcatgatg aagcgcacac atacctgttc agaattgaga aagcagcata
     1141 tcggtcagtc agccgttctc ctcggctggg tccacgggcg tagagatcat gggggactga
     1201 tcttcattga cttgcgtgac cgcgaaggta ttacccaggt ggtcttccac cctgagccga
     1261 atcgttccgt acatgaattg gcgcaccagc ttcgctccga atacgtgatc ggtgtcagcg
     1321 ggtcggtcca tgaacgtccc tcgggcacgg ctaatccaaa gctcgcgacg ggagagatcg
     1381 agctgattgc ggacaaactg gagattttaa atccggccaa gacaccgccc tttcaaattg
     1441 aagacgaatc ggccgttgcc gaaccgatca agttgaaata tcggtatctg gacctccgcc
     1501 gtcccgcgat gcagaaaaat ttcaggcttc gccaccagct gaacaagacg atccgttcct
     1561 tcctggataa gaaaggcttt cttgaaatcg aaacgccttt tctcaccaag agcaccccgg
     1621 agggagcccg cgactatctc gtgccgagcc gcatcaatcc gggccggttt tatgccctgc
     1681 ccca
//
LOCUS       DATMKH010000627         1875 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2195073, whole genome shotgun sequence.
ACCESSION   DATMKH010000627 DATMKH010000000
VERSION     DATMKH010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2195073"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VNV63_07510"
     CDS             <1..235
                     /locus_tag="VNV63_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62505.1"
                     /translation="EDLEIRGPGEFFGTRQSGLPELRIANVLRDAKVLEEAREAAFKL
                     IRQDPQLSHPAHQGLKTSLIRKWQDRLELITIS"
     gene            325..585
                     /locus_tag="VNV63_07515"
     CDS             325..585
                     /locus_tag="VNV63_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62506.1"
                     /translation="MSLFVEGAEQASLLNYKIQLVQLDHQLQDSYRDIGEKLFLKLSS
                     QQDETTENLDLTSDEKLQQLFGTVARLEQKKKMLLEEMDELR"
     gene            590..1546
                     /locus_tag="VNV63_07520"
     CDS             590..1546
                     /locus_tag="VNV63_07520"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HXC62507.1"
                     /translation="MTVMAGIDIGTNTLRLLIAKLNNPNELIELDSGRCITRLGEGMH
                     QDGTLSSFAIARTVSVLKEFKKKISDYKVDDLVVVGTSAAREAKNREEFLGRVKEETG
                     FEVELISGLEEARRTFLGVLLGVGPIQQHRIVVMDIGGGSTEFIIGEHGKPGQIVTTD
                     LGVVTLTERYLTNDPISAHDFSALQAAIESRLKPVCDSLISEKPFSLIGTAGTITTLA
                     AVDQRLDRYHPCQINRYRLKKTSVARMLKEFLSKTAGERSLMPSLEKGREDIIVAGTV
                     ILMTTMSRLGCSELLVCDYGLQEGILLDHYLNKHETNKEKEA"
     gene            1678..>1875
                     /locus_tag="VNV63_07525"
     CDS             1678..>1875
                     /locus_tag="VNV63_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62508.1"
                     /translation="MRERNRIIKVLLFSMLIFITGSSVSQAVDKGKETKLPMVTIETE
                     KGKIIFEMYPDVAPKTVARITE"
BASE COUNT          535 a          398 c          525 g          417 t
ORIGIN      
        1 ggaggacctt gagatccgcg ggccgggtga gttcttcggg acccgccaat caggcctgcc
       61 ggaacttagg atcgccaatg tccttcgaga tgcgaaggtc ctggaggaag cgagggaggc
      121 ggcttttaag ctcatccggc aggatcctca gctttctcat ccggctcatc agggactcaa
      181 aacatctctc attcgaaaat ggcaggatcg tttagagtta atcacgatca gctaggaaaa
      241 aacggcaaag gcatgcgaat attcataaca agacaacatt gggaagatct ttgtgaaggc
      301 ctgcggatct taaaggcggg cgccttgtcg ttatttgttg agggtgcaga acaggccagt
      361 ctgttgaact acaaaatcca actggtccaa ctggatcatc agttgcagga tagttaccgc
      421 gatattggag aaaagttatt cttgaaactg tctagtcaac aggatgaaac gaccgagaat
      481 ttagatttaa cttcggatga aaagctgcag caacttttcg gaaccgtggc tcgactggaa
      541 cagaagaaaa aaatgctgct tgaagagatg gacgagctga gataaagata tgacggtcat
      601 ggccgggatt gacattggga cgaacacgct ccgactgctc attgcaaagt tgaacaatcc
      661 gaatgagctg atcgagctgg attcaggtcg ttgcattaca cggctgggag aaggcatgca
      721 tcaggatggg acgctatcgt ctttcgccat agcacgaacg gtgtcggtcc ttaaagagtt
      781 caaaaagaag atttcagatt ataaagtaga cgatctcgtt gtcgttggaa ccagtgccgc
      841 acgcgaggcc aaaaacagag aggagtttct aggccgggtc aaagaggaaa cgggttttga
      901 ggtggaattg atctctggtc tggaagaagc ccgccgaacc tttctggggg tgttgttagg
      961 cgttggacca attcagcagc atcggatcgt agtcatggat atcggcggcg gaagcacgga
     1021 gtttatcatc ggcgagcacg gcaaacccgg tcagatcgtg acaaccgatt tgggggtggt
     1081 caccctgact gagagatacc tgacgaatga tccgatctct gctcatgatt tttctgccct
     1141 ccaagcagcg attgaaagcc ggttgaaacc tgtctgtgac tcgctcattt ctgagaaacc
     1201 gttcagcctc atcggaactg cggggacgat tacaaccctg gcagccgtgg atcagcgact
     1261 cgaccgttat catccgtgcc agatcaatcg ttatcggtta aagaaaacct ctgttgctcg
     1321 catgctgaag gagtttcttt ctaaaaccgc cggagagcgt tctctaatgc catctctgga
     1381 aaaaggtcgt gaggacatca tcgtggccgg cacagtaatc ttgatgacaa ccatgagccg
     1441 gctgggatgc agcgagctcc tggtgtgtga ttacggactg caggagggga tactgttgga
     1501 tcactattta aacaagcatg aaacaaataa ggaaaaagag gcttaagtcc ggccagcgaa
     1561 ggaggtggtt gagaaaaccg gtcacgcgag cgcattcgac aaagaagggt gaaaagggat
     1621 atgatcgaga taaatttaaa aaagagttaa gcaaagcctt ggaggagata ttaaaatatg
     1681 agagaaagaa acaggatcat taaggtactg ttatttagca tgcttatctt cattacaggc
     1741 agctcggtgt cgcaggctgt tgacaaggga aaggagacaa agttacccat ggtcacaatt
     1801 gaaactgaga aagggaaaat catcttcgaa atgtaccccg acgtggcgcc caagacggtg
     1861 gcccgcatca cggaa
//
LOCUS       DATMKH010000628         3234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_292236,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000628 DATMKH010000000
VERSION     DATMKH010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3234
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292236"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /locus_tag="VNV63_07530"
     CDS             <1..644
                     /locus_tag="VNV63_07530"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HXC62509.1"
                     /translation="DFPTLLGNSALYSATGAALAVLLALVPAYGLSRFEIPGKKLIFG
                     ILLMGLMLPQQTVLIPLYTTLRTLHLLDTKIGLILVHGVYGMPMQILALRGFMTSIPR
                     EIDKAAFIEGASDFQVFWKIILPLSIPGILVSYTLNFIAIWKEFVFSLVFLNNESNFP
                     VTVGMLKLNSDRYMSAFNLPSAGLVISQLPIILLFLILYRKFSGGKLAGAVKG"
     gene            675..1784
                     /locus_tag="VNV63_07535"
     CDS             675..1784
                     /locus_tag="VNV63_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003387467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXC62510.1"
                     /translation="MATISLVHIEKSFGPHKVISNLNLEVEDGQFVVLVGPSGCGKTT
                     TLNMIAGLESISAGSLLIGDQNVTNLPPKHRNIAMVFQSYALFPHLNVRENVAFGMKI
                     RHSPKEEIAEEVAKVTGRLRIKQYLDRLPKALSGGQRQRVALARALVRRPGVFLMDEP
                     LSNLDAQLRVEARSLLAKMHLDLKTTTIYVTHDQAEAMTLGDRVVVMNEGVIQQNAPP
                     LEVYNAPANRFVASFIGSPAMNFFDFRYADGELLDETQNLRIMLPPERLNGLDRFQNK
                     ALVVGARPEHIRTAQRLDRQTIRFTIDVAQQLGHETLLDVSNAGKSAVIRVAPTERFS
                     LGETRAFELDLEKIQFFDPETGVNVGRSLPPVSGS"
     gene            1877..3169
                     /locus_tag="VNV63_07540"
     CDS             1877..3169
                     /locus_tag="VNV63_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003592653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HXC62511.1"
                     /translation="MNKRKFLAILLTSALFISPGRTEAQAKKQITVWSWFISSTMSKS
                     IKAFEEKNPGLEVKYTYYNYSPEYITALKAAAASNSLPDIIGLQPGSLTQQYREQLAA
                     LNSHAEKEWGTDWQSKIFPVNLKQMAMGNPTGDTNFYILPQESQILVIWYNKELFKKL
                     SLEIPKTYKDLIAASKKLTGAGYIPLYQGAADGWQNENVFLMLANQLAPGLVDEAQNG
                     KSPWTSPPLIKAMQAWANLFKDGVFQQGALGAHAYPTGAQLFAQGRVGMMALGSWWMQ
                     ESKFPPPLSQYVQNMSGFDFFYFPALEENGKSSPPVGGIDIGYGMTKNGEKNPDAWKF
                     LASLVRGDGLQQALNDLNDLPATSDFEPQGEVTPNIKEMYGRFMADLPHAENQRFLSP
                     QVSEELDSVLGGVAAGSVTPEDGLKRVQAATDKALGKN"
BASE COUNT          772 a          869 c          852 g          741 t
ORIGIN      
        1 tggatttccc gacgctgcta ggaaacagcg ctctttactc cgcgacagga gcggcgctgg
       61 ccgtgcttct cgcgctggtg ccggcttacg gcctcagtcg cttcgagatc ccgggaaaaa
      121 agcttatttt cggaatccta ttgatggggt tgatgctccc ccaacagacc gtgctgatcc
      181 cgttgtacac gacacttcgc acccttcatc tgcttgatac caagatcgga ctcattcttg
      241 tgcacggagt gtacgggatg ccgatgcaga tattggcgct gcgcggattt atgacctcca
      301 tcccgagaga aatcgataaa gctgcattta tcgaaggggc cagtgacttc caggtctttt
      361 ggaaaattat cctcccactc tccatcccgg ggatcctggt cagctataca ctgaacttta
      421 tcgccatctg gaaagaattc gtcttctccc tggtctttct taacaacgaa tcgaattttc
      481 cagtcaccgt cggaatgctg aagttgaata gtgaccgcta tatgtccgcc ttcaatcttc
      541 cttccgcggg cctggtgatt tcgcagctcc caatcatcct tctgtttctg attctttatc
      601 gaaaattctc cggaggcaaa ttagcgggcg cggttaaggg atagcccgct ctcctctcat
      661 ccgttgacgc gttcatggca acaatatcgc tggttcacat tgaaaaatcg ttcggaccgc
      721 acaaggtgat ttccaatctc aacctggagg tggaggatgg tcaatttgtc gttctcgtcg
      781 gaccgtctgg ctgcgggaaa acgaccacgc tcaatatgat tgccggcttg gaatcgatca
      841 gcgccgggag cctgctgatc ggggatcaaa atgtcaccaa ccttcctcca aagcatcgca
      901 atattgccat ggtttttcaa tcgtatgccc tcttcccgca cttaaatgtg cgcgagaatg
      961 tggcgttcgg aatgaagatc cggcatagcc cgaaagaaga aatcgcggaa gaagtcgcca
     1021 aagtgaccgg acgcctgcgc atcaaacagt atctggatcg gcttcccaag gcactgtccg
     1081 ggggacaacg acagagagtg gccctcgcac gggccttggt gcggcgccca ggggtctttc
     1141 tgatggatga gccattgtcg aatctggatg cccaactcag agtcgaagcc cgcagcctgc
     1201 tggcaaagat gcatctcgat ctcaagacga cgacgattta tgtcacgcat gatcaggccg
     1261 aggcgatgac cttgggggat cgtgtggtgg tgatgaacga aggtgttatt caacaaaatg
     1321 cgccgccgct cgaggtttac aacgctcccg ccaaccgctt tgtagccagt ttcatcggat
     1381 cacccgcgat gaatttcttc gattttcgct atgccgatgg cgagctgttg gacgaaactc
     1441 agaatcttcg gattatgcta ccgccggagc gtctgaatgg cttggaccga tttcaaaaca
     1501 aagctctggt ggtcggggca cggcccgaac atattcgaac tgcccagcgg ttggatcgcc
     1561 aaaccatccg attcacgatc gatgtcgcgc agcagctcgg tcacgaaacg cttctcgatg
     1621 tcagcaacgc cggcaaatcc gcagtgatcc gggtggcgcc cacggagcgg ttttctctcg
     1681 gagaaactcg agcattcgag cttgacctgg agaagattca attttttgat ccggagacgg
     1741 gagtgaacgt tggacgatcc ctcccacctg tttccggctc ctgaccacac cgcccagctg
     1801 tttctgaaag aaatatctat ctctgaaaaa actagatcaa ctcatcatcg agcgttcgat
     1861 caccaagagg tacctcatga acaagcgaaa attcctggct atcctgttaa caagtgctct
     1921 cttcatttcc ccgggccgaa ccgaagccca agccaaaaaa cagatcacag tgtggtcctg
     1981 gttcatctcc agcaccatgt ctaaatcgat caaagcattc gaggagaaga atcccgggtt
     2041 agaagttaag tatacttact ataactattc tcccgaatac atcaccgcgt taaaggcggc
     2101 cgcagcttcc aatagcttgc cggacatcat cggccttcag ccaggatctt tgacccagca
     2161 gtaccgcgaa cagttggccg ctttaaattc acatgcggag aaggagtggg gcacagattg
     2221 gcaatcgaag atctttccgg tgaatcttaa acagatggcg atggggaacc cgaccggtga
     2281 cacaaatttt tatattctgc cgcaggaatc ccagatcctc gtgatttggt ataacaagga
     2341 gctctttaaa aagttgagcc tcgagatacc gaagacctat aaagacctga tcgccgcttc
     2401 caagaagctg accggcgccg gatatattcc actttatcaa ggcgctgccg acggctggca
     2461 aaatgagaat gtatttttaa tgctggcgaa tcaactcgct cccggtctgg tggatgaggc
     2521 gcaaaacgga aaatcaccct ggacgtcgcc gccccttatt aaagcgatgc aggcatgggc
     2581 gaatcttttt aaagatggcg tattccagca aggggccctc ggggcgcacg catacccgac
     2641 gggggcccag ctgtttgccc agggccgggt tggaatgatg gcactcggtt cctggtggat
     2701 gcaggagagc aagttcccgc ctcctctctc tcagtacgta cagaacatga gcggcttcga
     2761 tttcttctat tttcctgcgc tggaagagaa cggaaaatcg agcccgccgg tcggaggaat
     2821 tgacattggc tacggcatga cgaagaacgg cgagaagaat ccggacgcgt ggaagtttct
     2881 agcgtctctg gtccgagggg acggcctcca gcaagctctc aacgatctga acgatttgcc
     2941 cgccaccagc gattttgaac cccaaggaga ggtcactcca aacatcaagg aaatgtacgg
     3001 gcggttcatg gccgatcttc cgcatgcaga aaatcagcgg tttcttagtc cgcaagtatc
     3061 cgaagaactc gacagcgtcc ttggaggggt ggcagcgggc tcggtcactc cagaagatgg
     3121 tttgaagagg gttcaggcgg ccacggataa agccctgggc aagaattgac gaatgcgcgc
     3181 aagacgtgga tcgtggctcg aacggtcgcc ctatctgtac gtcttgccgg cgac
//
LOCUS       DATMKH010000629         1060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3481852, whole genome shotgun sequence.
ACCESSION   DATMKH010000629 DATMKH010000000
VERSION     DATMKH010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3481852"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..>1060
                     /locus_tag="VNV63_07545"
     CDS             113..>1060
                     /locus_tag="VNV63_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018398726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide ABC transporter ATP-binding
                     protein"
                     /protein_id="HXC62512.1"
                     /translation="MLDPAITIENLGKRYTIGHQRTNGDGMRHAIEGAMRAPLAWVRS
                     RREKKLKEADFWALKDVSLEIKQGEVVGLIGRNGAGKSTLLKILSRITVPTEGRIRIH
                     GRIASLLEVGTGFHQELTGRENIFLNGAILGMPRAEIVRKFDEIVEFSEIGEFLDTPV
                     KRYSSGMYVRLAFAVAAHLEPEILIIDEVLAVGDTAFQKKCLGKMGSFAQSGRTVLFV
                     SHNMEAVRNLCQKVIWLKDGRLHQDGKANEIIEAYFSSISNELSFSFANADYGLIIQK
                     VVLKNELGKESSQFRPGDDLVVEIFYDAQKRIEKPILALG"
BASE COUNT          267 a          273 c          293 g          227 t
ORIGIN      
        1 tgattgacgt gatcgtcctg gttacgtccg gcgtctggta tttccgaaag accgagcaga
       61 cttttgcgga cgtaatatga gagacgccga ggcacctgcc aaagccgccg caatgctcga
      121 tcctgctatc accatcgaga atctcggtaa acggtacaca atcggtcatc aaaggaccaa
      181 cggtgatggt atgcgccatg ccatcgaggg tgcgatgcgc gcgccactcg cctgggttcg
      241 atcccgccga gaaaagaagc taaaagaggc ggatttctgg gctctaaaag atgtgtccct
      301 tgagatcaag cagggtgaag tagtcggcct catcggtcgc aacggtgcgg gcaagagcac
      361 cctattgaag atcctgagcc gaattactgt tcctaccgaa ggacgaatcc gcatccacgg
      421 ccggatcgcc agccttcttg aggtgggcac cggatttcat caggaactca ccggacggga
      481 gaacatcttc ctgaacggtg caatcctggg catgccccgt gcagagatcg tccgcaagtt
      541 tgacgaaatc gtcgaatttt cggaaatcgg agagtttctc gacactcccg tcaaacgcta
      601 ctcttctggc atgtatgtgc gcttggcctt tgcggtggcg gcccaccttg agcccgaaat
      661 cctgatcatc gacgaagtgc ttgccgtcgg cgacacagct ttccaaaaga aatgcctcgg
      721 aaagatggga agctttgccc agagcggaag aacggttcta ttcgtaagcc acaatatgga
      781 ggcagtccgg aacttatgcc aaaaggtcat atggcttaag gatggccggc tgcatcaaga
      841 cggaaaggcg aatgaaatca tcgaggccta tttcagcagc atctccaatg aactctcttt
      901 ttccttcgca aatgcggatt acgggttgat cattcagaag gtcgtgctca agaacgaatt
      961 ggggaaggaa agcagtcaat ttcgtcccgg cgacgacttg gtcgtagaaa tattctacga
     1021 tgcgcaaaaa cggatcgaga agcctatcct agcgctgggc
//
LOCUS       DATMKH010000630         1071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4755964, whole genome shotgun sequence.
ACCESSION   DATMKH010000630 DATMKH010000000
VERSION     DATMKH010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4755964"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="VNV63_07550"
     CDS             complement(<1..344)
                     /locus_tag="VNV63_07550"
                     /inference="COORDINATES: protein motif:HMM:NF014417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HXC62513.1"
                     /translation="MTPPSATKLSWYALRVRSRCEQSVASAVRAKGFEDFLPLYDSLK
                     RWSDRTKSVREPLFPGYIFCRLGANHRFPILTIPGALYFVGIGKTPVPVEDSEMEAIQ
                     GIVRSQLRIESRP"
BASE COUNT          274 a          293 c          273 g          231 t
ORIGIN      
        1 ggccggctct cgattctaag ctgagatcgg acgatgccct gaatcgcttc catctccgaa
       61 tcctcaacgg gcacaggcgt ctttccgatt ccgacaaaat acaaagctcc aggaatggtc
      121 agaatcggaa accgatgatt ggcccccagc cggcagaaaa tgtatccagg aaacagcggt
      181 tcccggacag acttggttcg atccgaccaa cgcttcaggg agtcatataa cggaaggaag
      241 tcttcaaacc cttttgctcg gacagcggag gcgaccgatt gctcacacct cgacctcacc
      301 ctgagcgcgt accaactcag cttcgtggcc gatggcggtg tcatggtgtc cataaggttg
      361 ccgcttcagc agtagctctt tcccgggaaa ccctctgcga aactaaggct gcgtataatc
      421 ccgcgttcgc gacctggcca cagtggcatt tacgccagcc acgtaccgct gctgagagcc
      481 aactcagggc cattgtcggg tttcgaatgg ttgtaacccg actagggcgc aagcggagag
      541 tgagccaaaa ccgctccccg cgggatcgcg taggtgtgat caccgcctcc agaagctcga
      601 ttccaagcgg accttgaaga tctaatatat tatttttcaa taacttaaga tcagaagcag
      661 agcgcatccg gacaagctac cgcccttcca gtcccacatc caagttcttt ttggaggggg
      721 aggtcggttt ggctgcgttg aactgacaac tagagcaact cctttgccat ctcgaaatca
      781 accgagcatt cctatatcgt tctatggttt gcacgcttcc ccgaggagaa aaaagagagg
      841 caatgagttt gaaacgaata ctattcggat ttcatcccaa attgaaggat tctgggacat
      901 ggcaatcaga ctggatcgat gcgtctgagg aaagctgctg ctaactactt aaattctttg
      961 agtttccgcc gcgaacatgg atccaaacac ccaagaacct accgaagagg agcacaccaa
     1021 gccaagtgcc aagggtattg gtcaggacgt ctgtgaggtc agagtcccga g
//
LOCUS       DATMKH010000631         2696 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1254540, whole genome shotgun sequence.
ACCESSION   DATMKH010000631 DATMKH010000000
VERSION     DATMKH010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1254540"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VNV63_07555"
     CDS             complement(<1..216)
                     /locus_tag="VNV63_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62514.1"
                     /translation="MLTPRYVQDSETLIKAAKRILNYRKDVTAADQLEEARVGIARVR
                     SAIKQRSAPAVKEAEKPLVEVLTKIDPP"
     gene            complement(221..2017)
                     /gene="lepA"
                     /locus_tag="VNV63_07560"
     CDS             complement(221..2017)
                     /gene="lepA"
                     /locus_tag="VNV63_07560"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HXC62515.1"
                     /translation="MGRLLTRNFCIIAHIDHGKTTLSDRLLEFTGTIADRDKQDQLLD
                     SMDLERERGITIKAHPVAMKYSAKDGQTYLLNLLDTPGHVDFSYEVSRSLAACEGAIL
                     VVDAAQGVEAQTVANVHLALKQRLTIVPVINKIDLPNADIPTVRKQLEEILAIPADEA
                     ILASAKTGLGIEEILEAIVARIPPPPAPEDNKLRTLIFDSVFDIYRGVVAYSRVFSGR
                     IVAGQAVKLMSTGKSYEIKEVGIFTPKPLAQESLEEGDVGYFIANIKSTSEIKIGDTL
                     TEQRNPAPEQLPGFQEIHPMVFSGIYPINTADFEHLKTALGKLQINDSAFVYQAENSV
                     ALGFGFRCGFLGLLHMEIVQERLRREFDMDIIATYPSVVYEVQQTNGEKVEVDNPAHL
                     PDPSVIDEIREPVVECFVMCPNENIGDMMQLIMEKRGEVQHTESLDTKRVMLTAIMPL
                     NEILVDFNDRIKSLTHGYGSMDYEHSGYRPADLVKLDMLVNGEPVDAFSSIVHRDKAE
                     GRGRLLAAKLKEVIPQQLFQVSIQAAIGGKVVARENVSALRKNVTAKCYGGDITRKRK
                     LLEKQKEGKKRMKAIGKVNIPQEAFIEVLKTN"
     gene            2277..>2696
                     /locus_tag="VNV63_07565"
     CDS             2277..>2696
                     /locus_tag="VNV63_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62516.1"
                     /translation="MLADVLSPIYFSPPGLLVCSLLSLAGIVLIEGTVLRLLRWGSWK
                     ITFLHAFIINLITSLLGTGLLFLASNLPILTGPGLFGFAFLLTFALGAFLLTVIVEAG
                     VLKALRQSPSFSRILLNSLVANFFSYLFLLVMICLVLA"
BASE COUNT          561 a          764 c          646 g          725 t
ORIGIN      
        1 aggtggatct atcttggtca atacctccac cagtggtttt tcggcttctt tgaccgccgg
       61 ggccgatctt tgctttatcg cactacgtac acgagcaatc ccgactcgtg cctcttccag
      121 ttgatcagca gcagtgacat ctttgcgata attaagaatt cgttttgccg cttttattaa
      181 ggtctcggaa tcctggacgt atcttggtgt taacacggtg ctagtttgtt ttcagaactt
      241 caatgaaggc ttcctgcgga atattcactt tgccgatcgc cttcattcgc ttctttccct
      301 ctttctgttt ctccaagagt ttccgcttcc gcgtgatgtc accgccgtaa cacttggccg
      361 tcacattttt acgcagcgca gaaacgtttt cacgggcgac aaccttgccg ccaatggcgg
      421 cttgaatcga tacttggaaa agttgttggg ggatgacttc cttcagtttg gctgccagca
      481 accggcctcg gccttcagcc ttgtcccgat ggacaatact cgaaaaagcg tcaaccggtt
      541 ccccgtttac cagcatgtcc agtttcacca ggtcagcggg acggtagccg gagtgctcgt
      601 aatccatgga tccgtatccg tgggtcaacg atttgatcct gtcgttaaaa tccaccaaaa
      661 tctcgttcaa cggcatgatg gcggtcaaca ttacccgctt cgtgtcgagc gattctgtgt
      721 gctgaacttc gccccttttc tccatgatca gctgcatcat atcgccgatg ttttcgttcg
      781 gacacatcac gaagcactcc accaccggct ctctaatctc gtcgatcacg ctcggatccg
      841 gtaaatgggc ggggttatca acttcgacct tctccccgtt ggtctgttgc acctcgtaaa
      901 ccacgctcgg gtaggtcgcg atgatgtcca tatcgaactc ccgacgtagt cgctcctgta
      961 ctatctccat gtggagcaaa ccgagaaaac cacaacgaaa accgaagcct aaggccacgg
     1021 aattctccgc ctgatagacg aaggcggaat cgttgatctg cagttttccc agggcggttt
     1081 tcagatgttc gaaatcagcg gtatttatcg ggtaaatccc gctgaagacc atcggatgaa
     1141 tctcttgaaa cccaggtaac tgctccggtg ccggattgcg ctgttcggtc agggtgtccc
     1201 cgatcttgat ttccgacgtg gatttaatat tggcgatgaa atagccgacg tcaccttctt
     1261 ccaagctctc ctgagccagc ggcttcggag taaaaattcc tacctctttg atttcgtagc
     1321 tcttaccggt gctcattagc ttgacagctt ggcctgcgac gatcctaccg gaaaacacgc
     1381 gcgaataagc caccacccca cggtagatgt cgaacaccga gtcaaagatc aaagtacgga
     1441 gcttgttgtc ttctggcgct ggaggaggcg gaatccgggc aacaatcgct tcgaggatct
     1501 cttcaatgcc caaaccggtc ttggcgctgg ccagaatggc ttcatcagcc gggatggcca
     1561 ggatttcctc cagctgtttt cgaaccgtgg gaatgtccgc gttgggcaga tcgatcttat
     1621 tgattaccgg aacgatggtc agtcgctgtt tgagcgccaa atgcacgttt gccactgtct
     1681 gcgcttccac cccctgagct gcgtccacca ccaggattgc tccctcacaa gcagctaagc
     1741 ttctcgatac ctcgtacgaa aaatcgacgt gcccgggtgt atccaacaag tttagcaggt
     1801 aggtctgccc gtccttggct gaatatttca tggcgaccgg atgagccttg atggtaatgc
     1861 ccctctcccg ttcgaggtcc atcgaatcca gcaactgatc ctgcttgtct ctgtcggcga
     1921 tcgtaccggt gaactccagc aaccggtcag aaagcgtcgt cttcccgtga tcgatgtgag
     1981 cgatgataca aaaattccga gtcaaaagtc ttcccatgca gagggcgcac tatacaggag
     2041 gcaatcgcag aatccaggga ggaagcacgg gtggcgaccg aatggggtgg cgcgaagtaa
     2101 tcagtaaatc agtaatcagt actgaggttt ccccagatgg ctctgcggaa atccggtctg
     2161 attactggca cactgattac tgattgcttt gcgccacccc ccatcggtca cctatcacct
     2221 agaaagtaca tccccgcctt ttcaatcatc tccttgctct gatatcatcg actcaaatgc
     2281 tcgcggatgt gctttcgccg atctacttta gcccgcctgg gcttttagtg tgctctctgc
     2341 tcagcttggc gggcatcgtt cttatcgagg ggacggttct tcgcctcctc cgctggggct
     2401 catggaaaat cacgtttctc cacgcattca tcattaactt gatcacttct ctgctgggaa
     2461 caggcctgct tttcttagcc agcaacctgc ctattctcac cggcccagga ctatttggtt
     2521 ttgccttcct gctaaccttc gcgctaggag cctttctgct aaccgtcatc gtggaagcgg
     2581 gagtgctgaa agcactccgg caatctccct cgttctctcg catcctcttg aattcgttgg
     2641 tggccaattt cttctcttat ctcttcctct tagtgatgat ctgtctggta ctagct
//
LOCUS       DATMKH010000632         1152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_936111,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000632 DATMKH010000000
VERSION     DATMKH010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_936111"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VNV63_07570"
     CDS             <1..225
                     /locus_tag="VNV63_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62517.1"
                     /translation="AASFKEIQWLKVASKSVGSIAGIHRGIRRIPIIPMGLISIAPRE
                     PLKPVLSISRIRLRDVAGMSSSVGYVVRST"
     gene            complement(328..954)
                     /locus_tag="VNV63_07575"
     CDS             complement(328..954)
                     /locus_tag="VNV63_07575"
                     /inference="COORDINATES: protein motif:HMM:NF025637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HXC62518.1"
                     /translation="MLDAVLETHRVPPVHSLMTLVLNADYQALTTFPLSLIPATEAIT
                     RIWKERAQTVETWKDAFGDDRLFRSPSVIIPAPKVVVLKEYVSISSEPKFSRRNIILR
                     DRFICQYCAGKFSSNDLTFDHVIPKKRGGVTEWTNIVASCEPCNSEKGDIMPNLSGKK
                     GKGSWRPMRLPMRPTNAQLLKAGLEFLPEQMKEDFGSWLYWNVEIEKT"
BASE COUNT          288 a          309 c          258 g          297 t
ORIGIN      
        1 gctgcatcct tcaaggagat ccaatggctc aaggtcgctt cgaaatcagt tggatcgatc
       61 gcggggattc accgcggcat ccgccgaatc ccgattatcc caatgggatt gatttcgatt
      121 gctccaaggg agccgctcaa acctgtactg tcgatctccc gtatccggct ccgcgatgtg
      181 gctggtatgt cgtcaagtgt aggatatgtg gttcgctcaa cgtgattacg actgccggtc
      241 ggcccgatga tcccagatcg atcaaatttg cctgtgagtc attgattaag gcataagaaa
      301 agcgcccgac taacgggcgc gattccccta tgttttttcg atttccacat tccagtacag
      361 ccaggatcca aaatcttcct tcatctgctc tggaagaaac tctagaccag ccttcaggag
      421 ttgggcattc gtcggccgca tcggaagccg catcggtcgc caggaaccct tacccttctt
      481 accagaaaga ttcggcatga tatctccttt ttcagaattg cacggctcac aactcgctac
      541 aatgttcgtc cactccgtga ctcctccacg tttcttcggg atcacgtgat caaaagtgag
      601 atcgttgcta gagaacttac cagcacaata ctgacagata aaccgatcac gaagaatgat
      661 gtttcttcgt gaaaacttcg gctcactcga aatactcacg tattccttca gcaccacaac
      721 cttaggagcc ggaatgatca cactgggcga ccggaataac cgatcatcac caaaggcatc
      781 cttccaggtc tcaactgtct gcgcccgctc cttccaaatc cgcgtgatcg cttcggttgc
      841 cgggatcaga ctcaagggaa acgtggtcaa tgcttgatag tcagcattga gaaccagggt
      901 catcaatgaa tgaaccggcg gtaccctatg agtctccaat actgcatcta acatcacatc
      961 acgcttaccc cttcttctct atagtagtta ggctgaatcg aaaattccga ttcagtattt
     1021 ctcgggcagc cagcattttg ctgtctgacc cttcggggca gcgatctttt ggtttgacga
     1081 attgtaaata atcgatgaaa attgtcgaga ttaccacaga cgaatttatt caaagtggct
     1141 tgaatactgg ta
//
LOCUS       DATMKH010000633         1310 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4129055, whole genome shotgun sequence.
ACCESSION   DATMKH010000633 DATMKH010000000
VERSION     DATMKH010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4129055"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..>1310)
                     /locus_tag="VNV63_07580"
     CDS             complement(12..>1310)
                     /locus_tag="VNV63_07580"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HXC62519.1"
                     /translation="RAAVITGKVFDEDGEPMVGVTVRAMRQDFSNGRKGLGFANENLT
                     DDLGEFRLFGLAPGRYYISAQLSAWDHIVGDREFSGSEKNSGQKGYAKVYYPGALEAA
                     KASSIYVKEGEEVPAVDIFMKEVTVYTVHGKVQYLFPHQGTAETRLMVLRRGQRFDWD
                     LAAQQPVVKADGSFQIPEIPPGEYTVRAYFFDQEKLYSTREDFDVMNADVDGLTLSLA
                     QGTEISGHLLWDGKPSLEGERLSIFLSSDESAANDRGGWAHVEENNQFTMKDVSQGTF
                     RIDLIGVSKDCYIKEIRFGEASLPDHLLQVKRGLAGPLDITISSKGAKIQGTVTNAES
                     TPVAGVWVVAVPEESRRNLRDLYKSVTTDQYGHYDLRGLAPGKYLLFSWDGVETGEWE
                     DPEFLKINGAKGVAIEVSDADSKSVDLQLIELKNPAVASE"
BASE COUNT          277 a          382 c          308 g          343 t
ORIGIN      
        1 aacgggacat tctactcact tgcgactgcg ggattcttga gttcgatcaa ttgaagatcg
       61 acagatttcg agtcggcgtc gctcacttct atcgcgacac cttttgctcc gttgattttc
      121 aggaattctg gatcttccca ctctccggtc tccacgccat cccaactgaa taataagtat
      181 tttcccggtg cgagcccgcg aagatcgtag tgcccgtatt gatctgtggt cacagacttg
      241 tacaaatcgc ggaggtttct cctgctttct tcgggtacgg caaccaccca cactccggcg
      301 actggtgtac tttccgcgtt ggtcacggtg ccctggatct ttgctccttt ggagctgatg
      361 gtgatgtcca gtggaccggc cagtccccgt ttgacttgaa gcagatgatc tggcaaggaa
      421 gcttcgccga aacgaatttc cttgatgtaa caatccttgg aaactccaat caggtctatt
      481 cgaaatgttc cctgcgaaac atctttcatc gtgaattggt tattttcctc gacgtgggcc
      541 cagccgcctc gatcatttgc cgcggattca tcggacgaca gaaaaatgct cagccgttca
      601 ccttcaagac ttggcttgcc gtcccacagc agatgtcccg aaatctctgt cccttgtgcc
      661 agggaaagcg tcaaaccatc tacgtccgcg ttcatcacgt caaaatcttc ccgcgtcgag
      721 tacagctttt cttgatcgaa gaaataagcc ctaaccgtgt actctccggg cggtatttcg
      781 ggaatttgaa atgagccgtc cgctttcact acgggctgtt gagcagcaag atcccaatca
      841 aacctctggc ctcgccttaa gaccatcaaa cgtgtttcgg ccgttccctg atgagggaag
      901 aggtattgaa cctttccgtg caccgtgtaa acggtcactt ctttcatgaa gatgtcgacc
      961 gctgggactt cttctccttc cttaacgtaa atgctggacg cctttgcggc ctccagtgcc
     1021 cccggatagt aaactttcgc atatcctttt tgtcccgaat ttttctccga gcctgaaaat
     1081 tcacggtcgc ccacgatatg gtcccatgcg gacagctggg cagaaatata gtagcggccg
     1141 ggtgcgagtc cgaagagccg aaattccccg agatcatccg ttaggttctc attcgcaaac
     1201 ccaagccctt ttcgcccgtt gctgaaatcc tggcgcatgg cgcgcaccgt gacacccacc
     1261 atcggctcgc cgtcttcatc aaacaccttg ccggtgatca cggcggcgcg
//
LOCUS       DATMKH010000634         1692 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4463128, whole genome shotgun sequence.
ACCESSION   DATMKH010000634 DATMKH010000000
VERSION     DATMKH010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4463128"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="VNV63_07585"
     CDS             <1..355
                     /locus_tag="VNV63_07585"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HXC62520.1"
                     /translation="AWSIPKGVVDPGEDYLAAAKREFEEETSIPPSEPFIGLGEVRQK
                     SGKRIFVWAFQRSGATPGVKSNLFTLEWPPHSGLRKAFPEVDKGEFFSLPDALQKINR
                     SQAEFLARLETELAR"
     gene            complement(424..849)
                     /locus_tag="VNV63_07590"
     CDS             complement(424..849)
                     /locus_tag="VNV63_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62521.1"
                     /translation="MRSWILFLLVVSISGSASGRLGETEKQLKARYGRPISVRPGGKY
                     NPYFDKTIDFRKEGIAIACGLHAGRCVTIQYSRDSGFNTLEFEGFTLLNRLGPPVLVQ
                     RTATSLKYTFKDDYSEIVKGSQAGIRKMRQDVDSFKQKN"
     gene            1034..>1692
                     /locus_tag="VNV63_07595"
     CDS             1034..>1692
                     /locus_tag="VNV63_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019086015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXC62522.1"
                     /translation="MEYRQLGQSGLMVSVLSLGTMTFGGRTIFSKVGATDIDGARRQV
                     DLCLEAGVNLFDTANCYSDGVSEEILGEALEGRRDDLLIATKARMVVGPGPNDGGVSR
                     YHLIRECEKSLRRLRTDRIDLYQLHEWDGRTPLEEALEALDTLVRSGKVRYVGASNYS
                     GWHLMKALATSERRGYVRFVSQQIYYSLQARDAEYELIPVSIDQGLGILVWSPLAGGL
                     LS"
BASE COUNT          381 a          433 c          481 g          397 t
ORIGIN      
        1 cgcctggtcc atcccaaagg gagtggtcga tccgggcgag gattatttgg ccgctgcaaa
       61 acgggagttt gaagaagaga cttcgatccc tccttccgaa cctttcatcg gtcttggcga
      121 ggtaagacaa aaatccggaa aacggatctt tgtctgggcc tttcaacgaa gcggcgctac
      181 gcccggcgtg aaaagcaatc tttttaccct ggaatggccg ccgcactccg gattgcgaaa
      241 agcttttccg gaagtagaca agggcgagtt cttctccttg ccggacgctc ttcagaagat
      301 caaccgctct caagcggagt tcctggcgcg attggagacc gagctagccc gctaatgcac
      361 cagtcactga cttggtgcac tagtctcctg tccccgaaat acgcttattt cggggacagg
      421 agattagttc ttctgcttga aggaatcaac atcctgccgc attttgcgaa tacccgcttg
      481 cgaccctttc acgatctccg aataatcgtc cttgaacgta tattttaatg aagttgcagt
      541 tcgttgcacg aggaccggag gacccagacg attgagcagg gtgaagcctt cgaactccag
      601 ggtgttgaag ccggaatccc tcgaatactg aatcgtcacg catcgcccag cgtgaagtcc
      661 gcaggcgatc gcgatacctt cttttcggaa atcgatcgtc ttatcgaagt acggattata
      721 ctttccgcca ggccgaacgg aaattggcct tccgtatctg gctttcaact gcttctcggt
      781 ctctcccagc cggcctgacg cactgcctga aatgctgacg actagaagaa ataatatcca
      841 ggacctcatg atgcctttgc tatcagcgcg agcgcgttga ccttaaccat ttttgggagg
      901 gagcacaagc gctcaaggat gtccgaggtg ttcgacgagt caagaggcat taaataaaga
      961 gtccgaaaaa atggggcttt gtcctgctga aagatgttcg cagtcgattg actcgtgatg
     1021 tatggagtgg cctatggaat atcgtcaact cggacaatcc ggcctgatgg tctcggtgct
     1081 cagtttgggc acgatgacct tcggtggccg caccattttc agcaaggtag gagcgaccga
     1141 tatcgacggc gcgcgccgtc aggtcgatct ctgcctggaa gctggcgtca atctgtttga
     1201 tacggccaac tgctattctg acggtgtatc ggaggaaata ctgggcgagg ccttggaagg
     1261 gcgtcgagat gatctgctca tcgcgacgaa ggccaggatg gtggtaggcc cgggaccgaa
     1321 cgacggcggg gtatctcgct accatctgat ccgagaatgc gaaaagagcc tgagacggct
     1381 acgcacggat cggatcgatc tctatcagct tcatgagtgg gacggccgaa caccgctgga
     1441 ggaggctctt gaggcgttgg ataccctggt acgctccggt aaagtgcggt atgtcggggc
     1501 gtcaaactat tccggatggc atttgatgaa agcgctggcc acctccgaac gccgcggtta
     1561 tgtgcggttt gtcagccagc aaatttatta ttctctccag gccagggatg cggaatatga
     1621 actaattcca gtctcgatcg atcaagggct ggggattctg gtctggagtc cgctcgcagg
     1681 tgggctttta tc
//
LOCUS       DATMKH010000635         1386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1256141, whole genome shotgun sequence.
ACCESSION   DATMKH010000635 DATMKH010000000
VERSION     DATMKH010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1386
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1256141"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="VNV63_07600"
     CDS             complement(<1..270)
                     /locus_tag="VNV63_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009188754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HXC62523.1"
                     /translation="MSNPTNQPFLWGAATASYQIEGAVAEDGRAPSIWDTFSHTPHKI
                     ERSETGDIACDHYYRHTEDIQLMRNLGIQAYRFSISWSRILPEGTG"
     gene            complement(333..1205)
                     /locus_tag="VNV63_07605"
     CDS             complement(333..1205)
                     /locus_tag="VNV63_07605"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HXC62524.1"
                     /translation="MRHVILDTDIGTDVDDLLALVFLAKAPELCLEGVTTVHGDTLLR
                     AKIASITCQMLGIPDLPVVPGTSETLSGHPIFWPGHEGQNIPGIENARVETSISAQQF
                     LFGFSEKYPGELEILAIGPLTNLAQTISRFPDFRSLVKRLYVMGGAYWADYPEHNIEM
                     DVLAAKIVFESGIPITAIGLDVTLRVWVTEIQVERIRNLPNDLGPMLEGQIKRWWKFL
                     GRNQNNPHDPLAALAMVRPDLFSFATCDVAICMENDRRGHLIRRDNLNGKISAAYDLD
                     VHSAERLLLSYIAG"
BASE COUNT          328 a          355 c          369 g          334 t
ORIGIN      
        1 tcccgttcct tcgggaagta ttcgagacca ggagatggaa aagcggtagg cctggatgcc
       61 caggttgcgc atcagttgga tatcttcagt gtgccggtag tagtggtcac atgcaatatc
      121 gccggtttct gagcgctcga tcttgtgcgg agtatgagaa aaggtatccc agatcgaagg
      181 cgctcgtcca tcttcggcaa cagcaccctc gatctgatag ctggcggtgg ctgctcccca
      241 tagaaacggt tgattggtgg gattagacat acaacggtgc aaaaaaacta ctatcttagt
      301 gttggttcat ctccaagcga aaaccctctc ccttaccctg cgatgtagct gagcaacaat
      361 ctttctgcgc tgtgaacatc caagtcatag gcagcgctaa tttttccgtt cagattatct
      421 cgccggatca ggtgaccgcg ccgatcgttc tccatacaga ttgcgacgtc acaggttgcg
      481 aaggaaaaca ggtcaggacg aaccatcgcc aacgccgcta gtggatcgtg ggggttattc
      541 tggttcctcc ccagaaattt ccaccatcgc ttgatctggc cttcgagcat cggccctaaa
      601 tcgttgggaa ggttgcggat tcgctcgacc tggatctccg tcacccaaac tctcaaggtc
      661 acatccagac caatcgccgt gatggggatt cctgactcga acacgatctt ggccgcgagc
      721 acatccattt cgatgttgtg ctccggataa tccgcccagt aggcgccgcc catcacatac
      781 aatctcttga cgagagatct gaagtccggg aagcgggaga tcgtctgcgc aagattcgta
      841 agtggtccga tggccaggat ttcgagttcg cccggatatt tctccgagaa accgaacaag
      901 aattgttgag cggaaatgct cgtttcgaca cgagcattct ctatccctgg gatattctga
      961 ccttcgtgcc cgggccaaaa aatcgggtgc ccggagagag tttcactggt tcccggaaca
     1021 accggaagat ccggaatgcc aagcatctga caggtgatgc tggctatttt ggctcgaagt
     1081 agcgtatcgc cgtggacagt ggtcactcct tccaggcata gttccggtgc tttggccagg
     1141 aaaaccaggg caagcagatc gtcgacatcg gtgccgatat cggtatcaag aatgacatgg
     1201 cgcatttgtc ttgaagccga ctttcggcaa aaaatgagta atcaaacggg tgacaagcaa
     1261 aaccgagttc gcgatctcac tcgatgctga gggcagtcag cgcaatgttt accacgatca
     1321 tgaaaagtca aaggtcactg ttattggcgt ctcttttgtt cgcctggatt ggaatgttgc
     1381 ttggtt
//
LOCUS       DATMKH010000636         1080 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4757986, whole genome shotgun sequence.
ACCESSION   DATMKH010000636 DATMKH010000000
VERSION     DATMKH010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4757986"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..>1080)
                     /locus_tag="VNV63_07610"
     CDS             complement(183..>1080)
                     /locus_tag="VNV63_07610"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HXC62525.1"
                     /translation="GQPLGLLNRTVAEIASTDIPILILGESGTGKDAYARLIHRLSSS
                     SEGEFRKLCCSVLHPEQLLEQLHSNSPANPLNRPAGTWFLDGIDDLDQSCQRVLLSAL
                     PDGHGQKENANSCARLISSSCVDLEDTVASGSLRKELYFRINGICFKLPPLRERKEDI
                     GTFLDYFLRKHSQELKRPAPYLAQETLDVLMAYDWPGNIRQLENLVRKIVVLGNAQSA
                     MEEIRTATLGSAPTGAFRNASSLKVAAKAASKKAERQLILQALERTRWNRKKAARDLQ
                     ISYKSLLYKIKQIGVPSSKLEG"
BASE COUNT          248 a          272 c          303 g          257 t
ORIGIN      
        1 tccatacatc aatgcggatc aagtcttgcg gtccaatttt gtattcggaa tcgagcggga
       61 cagccgcttt ggcggattcg ccgggtttct tggcagtcgt ttcctgggcg ccggcgaacg
      121 gaacaatgag aacggcaacc aggaatgaca gtaatcgaag atacgaggtc atgcgtcctc
      181 catcagccct caagcttact gcttggcaca ccgatctgct taatcttgta taacagtgat
      241 ttatagctga tttgcaagtc gcgagcagcc tttttgcggt tccaacgggt tctctcgagc
      301 gcctgaagta ttaattggcg ctccgccttc ttggaagcgg ccttagcagc aactttgagc
      361 gaagacgcgt tgcggaaggc accggttgga gccgagccca gcgttgctgt ccggatctct
      421 tccattgctg actgggcatt acccagaacc acaatcttcc gcaccaagtt ctccagttga
      481 cggatgtttc caggccaatc gtatgccatc aggacatcaa gcgtctcttg ggctaaatag
      541 ggagccggcc gtttcaattc ctgagaatgc tttctgagga aataatcgag aaaagtgccg
      601 atgtcttctt ttctttctcg gagtggaggg agcttgaaac aaatcccgtt gattcgaaaa
      661 taaagttcct tgcgaaggct cccggatgcc acagtatctt cgaggtctac gcaactagag
      721 gaaatcagcc gagcgcaaga attggcattt tctttttgac catggccgtc tggaagagcc
      781 gaaagaagca ctcgctgaca agattgatcc aaatcgtcga tgccatccag gaaccaagtc
      841 cctgctggcc tgttcagcgg attcgccggt gagttggagt ggagctgttc gaggagctgc
      901 tccgggtgga ggacggagca gcagagtttc cggaactccc cctcggatga gctggaaagt
      961 cgatggataa ggcgggcata ggcatcctta cccgttccgc tctcacccag gatcaaaatg
     1021 gggatgtctg tggaggcgat ttcggccacg gtacggttga gaaggcccag gggttgacca
//
LOCUS       DATMKH010000637         4269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_614760,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000637 DATMKH010000000
VERSION     DATMKH010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 4269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4269
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614760"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..725
                     /gene="leuB"
                     /locus_tag="VNV63_07615"
     CDS             <1..725
                     /gene="leuB"
                     /locus_tag="VNV63_07615"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004855334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HXC62526.1"
                     /translation="TLKRDVIEGLDLLVVRELTGGIYFGRPKGIKPYRGGKKGINTEV
                     YTTKEIVRIAKVAFEIARRRRRKVTSVDKANVLESSELWRQTVIEVHQDYSDVALEHM
                     YVDNCAMQLVRNPKQFDVILTTNMFGDILSDEAAQLTGSIGMLPSASLGDSGKTAMYE
                     PIHGSAPDIAGQDKANPLATILSAAMMLKYSWGLDAAADEIEQAAVRVLSLGYRTADI
                     DMNGTKRVGTKEMGDLVVKELI"
     gene            725..1741
                     /locus_tag="VNV63_07620"
     CDS             725..1741
                     /locus_tag="VNV63_07620"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HXC62527.1"
                     /translation="MLAKKDQYNVAVVGATGAVGREMVEVLEERKFPVGQLHLFASER
                     SAGEMIPFQGVDHPIQILTKEAFYGVDIALFSAGAEISREYAPIAAKSGAVVIDNSSA
                     FRMVSEVPLVVPEVNRHALHGHQGIIANPNCSTVQMVVVLKPLHERSRIKRIVVTTFQ
                     SVSGTGKGAMDELLEETQALLSFQEVNPKVYPAQIAFNCLPHIDDFLPNGYSKEEMKL
                     VNETRKILEDPTIQVTATTVRVPVYVGHSESVNIETEKKLSANEARAILSTAPGVQLY
                     DDPQRKVYPTPLEVAGKDEVYVGRIREDESIPNGLNLWIVADNLRKGAALNAVQIAEE
                     LING"
     gene            1745..2671
                     /gene="nadA"
                     /locus_tag="VNV63_07625"
     CDS             1745..2671
                     /gene="nadA"
                     /locus_tag="VNV63_07625"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HXC62528.1"
                     /translation="MVSATSNTKVVEKIQRLKVERKAIILAHNYQLGEIQDVADFIGD
                     SLELSQKAAKTDAEVIVFCGVHFMAETAAILCPDRLVIMPDLMAGCSMSDMITTEAVR
                     KMKAQHPGAVVVCYVNTSAAVKAESDICCTSSNAVKVVNSIPKDKEIIFIPDQYLGQH
                     VERQTGRKMILYHGYCPTHVRIMAEELESLKEAHPDAQVLVHPECTQEVAGLADQVLS
                     TGGICRTAKESSAKTLIIGTEIGILHRLQKENPDKEFVPACKWCDCGHMKVNNLEKLL
                     WCLEDLKPVITVPDEIGKKAWKAIERMMAISV"
     gene            2710..2925
                     /locus_tag="VNV63_07630"
     CDS             2710..2925
                     /locus_tag="VNV63_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HXC62529.1"
                     /translation="MHGLGKLLILFGGVLALIGVLILFAGKLPWLGRLPGDILFERKN
                     FSFYFPLATSVLISLVLSMVLWLLSRR"
     gene            3003..4082
                     /locus_tag="VNV63_07635"
     CDS             3003..4082
                     /locus_tag="VNV63_07635"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HXC62530.1"
                     /translation="MAQETIRVAVFQDMKALNLKSDGDLTIHRSHGDLLFAKPVNDFH
                     IVPSDSGLLINQQLFSESAIRVGSSRGDIRVNGQIFGGVLDIINKHGLLQVVETLDLE
                     DYLQGVVPLELNSKWHPEVLKVQAIVARTYALYQKRQNNGKDYDLVATTNDQLYGGKG
                     VEDARAAEAVRTTRGQVLTYAGQVINSVYHSTSAGPTEDAREVWGTDLPYLHGVDCPF
                     DAQSPWYQWDRAIDLVTLEENLRRKGFPIGIIATMTPYRFSQAGRVTRVRILHSEGEL
                     ILRGEELRRILGYTVLPSSHFKIEAIGQQIHFTGMGSGHGVGLCQWGAKELAERGYSY
                     ERILTYYYPGVQVQDMADLETVKAP"
     gene            4079..>4269
                     /locus_tag="VNV63_07640"
     CDS             4079..>4269
                     /locus_tag="VNV63_07640"
                     /inference="COORDINATES: protein motif:HMM:NF014592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HXC62531.1"
                     /translation="MRLSEFDYSLSHDRIAQHPLPERDRSRLLVFDRKIGKVEHRIFS
                     DLIHFLKPTDVLVINNSRV"
BASE COUNT          994 a         1025 c         1279 g          971 t
ORIGIN      
        1 cgacgctgaa gcgggacgtg attgaggggt tggatctctt ggtggtacgg gaacttaccg
       61 gaggaatcta cttcggccgg ccgaagggga tcaagccgta ccgcggcggt aagaaaggaa
      121 tcaacaccga ggtctatacc acgaaagaaa tcgtacggat tgcgaaagtg gcgtttgaaa
      181 ttgcccgcag gcgccgtcga aaagttacct cggtggataa ggcgaacgtg ttggagtcat
      241 ccgagttgtg gcggcagacc gtgatcgagg tgcaccagga ctattcggat gttgcgcttg
      301 agcatatgta tgtagataac tgcgcgatgc agcttgttcg aaaccccaag cagtttgatg
      361 ttattctcac gaccaacatg ttcggagata ttttaagcga tgaggcggct caactgaccg
      421 gctcgatcgg catgcttcct tcggccagtc tgggtgattc gggtaagaca gcgatgtacg
      481 aaccgataca tggaagcgcg ccggatatcg cggggcagga taaagcgaat ccattggcca
      541 caatactttc ggccgcgatg atgctaaaat attcgtgggg gctggatgcg gccgcggacg
      601 agattgaaca ggcagcggtc cgagtgcttt ctctaggtta tcgtacggcg gatattgata
      661 tgaacgggac aaagcgtgta ggcacgaaag agatgggcga tcttgtggtc aaggagctga
      721 tctaatgctt gcgaaaaagg atcaatataa tgtcgcggta gtgggcgcga ccggtgcggt
      781 ggggcgagaa atggtcgaag tgctggagga gcgcaagttt ccggtcggcc agctgcacct
      841 ctttgcctcg gagcgttcgg cgggagagat gatccctttt caaggcgtgg atcatccgat
      901 ccagatctta acgaaagagg ccttttacgg tgttgatatt gccctgttct cggccggagc
      961 ggaaatcagc cgggagtacg cacccatcgc ggccaagtcc ggggcggttg tgattgacaa
     1021 cagctcggct tttcggatgg tgtcggaggt gccgctggtg gtgccggagg tgaatcggca
     1081 cgcactgcac ggtcatcaag gaatcattgc gaatcccaac tgctcgaccg tccagatggt
     1141 ggttgtgtta aagcctctcc atgaaaggtc tcggatcaag cgaatcgttg tcaccacgtt
     1201 tcaatccgtc tcgggaacgg gtaaaggggc gatggacgaa cttttagaag agacacaggc
     1261 gctcttaagc ttccaggagg tgaatccgaa ggtctatccg gcccagattg cctttaactg
     1321 tcttccccat atcgatgact tcctgccgaa cggttattca aaggaggaga tgaaactggt
     1381 gaatgagacc cggaagatac tggaagaccc aacgatccag gtgaccgcga caacagtgcg
     1441 ggtgccggtc tatgtgggcc attccgaatc ggtgaacatt gagaccgaga aaaaactctc
     1501 ggcgaatgag gcccgtgcga tcctgtctac cgcaccgggg gtgcagctct atgatgatcc
     1561 gcaacgaaag gtttatccga caccgcttga ggtggcgggg aaggacgagg tgtatgtcgg
     1621 ccggatacgg gaagatgaat cgatcccgaa cggactcaat ctctggatcg tggcggataa
     1681 ccttcgcaaa ggagcggcct taaatgccgt ccagattgcc gaggagttga tcaacggata
     1741 agatatggtg agcgcaacat caaacactaa agtggtcgag aaaatccaac gactcaaagt
     1801 cgagcggaag gcgatcatcc tggcccataa ttatcagttg ggggagatcc aggacgtggc
     1861 ggacttcatc ggcgattctc tggagctctc ccaaaaagcg gccaaaaccg atgccgaggt
     1921 catcgtcttc tgcggcgttc attttatggc cgagaccgcg gcgatccttt gtccggatcg
     1981 gctggtcatc atgccggacc tgatggccgg ctgctcgatg tccgacatga tcacgacaga
     2041 agcggtacga aagatgaagg cccaacaccc gggtgcggtc gtggtctgct atgtcaatac
     2101 ctcggcagcg gtgaaggccg agagcgatat ctgctgtacc tcctccaatg cggtcaaggt
     2161 ggtgaattcg atcccgaagg acaaggagat tatcttcatc ccggatcagt acctcggcca
     2221 gcatgtcgaa cgccagaccg gacggaagat gatcctgtat catggctact gcccgacgca
     2281 tgtccggatt atggccgagg agcttgagtc gctcaaagag gctcaccccg atgcccaagt
     2341 gctagtacat ccggaatgta cgcaggaggt ggccggtctc gcggatcagg tactcagtac
     2401 gggcggcatc tgcagaacgg cgaaggagtc atcggccaag accttgatta tcggaaccga
     2461 gatcgggata ctgcatcgtc tccagaaaga aaatcctgat aaagaattcg tgccggcttg
     2521 caagtggtgt gactgcgggc acatgaaggt caataatctc gagaagctac tctggtgttt
     2581 ggaggatctc aagcctgtga tcacagtgcc ggatgagatc ggcaagaaag cgtggaaggc
     2641 aatcgagcgg atgatggcga tttcggtata agcggtttaa aagtgtggag cgcaggaaca
     2701 gtgtcggagc tgcacgggtt gggtaaactt ttgatactct tcggcggtgt gctcgctctg
     2761 atcggggtcc tgattctttt tgcgggcaag cttccctggc tggggcggct accgggagac
     2821 atcttgtttg agcgtaaaaa tttcagtttt tatttcccat tggctacgtc tgttttaatc
     2881 agcctggtgc tttcaatggt cttgtggctc ctttctagac ggtgagaatg cgatttaatt
     2941 ctggaagatg cgccgtcctt ttcgcattct ttgtattatc ctctctcttt agcgatcatg
     3001 tcatggccca ggaaacgatc cgtgtggccg ttttccagga catgaaggcg ctcaatctaa
     3061 aatctgatgg cgatctgacc attcatcggt ctcacgggga ccttctcttt gccaaacctg
     3121 tgaacgactt ccacattgtc ccttccgatt cgggtcttct tatcaaccaa cagttgtttt
     3181 cagaatcagc gatccgggtc ggctcaagtc gcggcgatat ccgagtcaac ggccagatct
     3241 ttggaggcgt cctggacatt ataaacaaac acggtcttct gcaagtggtg gagactttag
     3301 atttggagga ttaccttcaa ggtgtggttc ctctggaact gaattctaaa tggcatccgg
     3361 aggtcctgaa ggtccaggcc atcgtggccc gaacctatgc cctgtatcag aagaggcaaa
     3421 acaatggaaa ggattatgat ctggtggcga cgacaaacga tcagttgtat gggggaaagg
     3481 gggtcgagga tgcccgggcc gcggaggcgg ttcgaacgac acgtggacag gttttaacct
     3541 atgcaggcca agtgatcaac tcagtttacc attctacttc ggcgggcccc acggaggatg
     3601 cccgagaggt ttgggggacg gacctcccgt atctccacgg tgtggactgc ccctttgatg
     3661 cccagtcccc ttggtatcaa tgggaccggg cgattgatct tgtcacgttg gaagagaatc
     3721 tccgaaggaa aggattccct atcggaatca tagctacgat gacaccttat cgcttcagcc
     3781 aggccggccg ggttacgcgc gttcgcatac tgcattcgga gggagagctc atactgcgcg
     3841 gcgaggagct ccggcgaatc ttgggctaca cggtactccc aagcagccat ttcaagattg
     3901 aggcgatcgg acaacagata cattttaccg gcatgggctc cgggcatgga gtagggctct
     3961 gtcagtgggg agcgaaggag ttggccgaac gcggctactc ttatgaacgg attctcacct
     4021 attattatcc gggggtgcag gttcaggata tggcggacct ggaaacagtg aaggcaccat
     4081 gagactgtcc gaatttgact attcattgag tcatgaccgt attgcccagc atcctttgcc
     4141 tgagcgagat cgatcccggc tcttggtgtt tgaccgcaag atcgggaaag tcgagcatcg
     4201 aattttttcc gacctgatcc actttcttaa gcccaccgat gtcctggtga tcaacaacag
     4261 ccgggtttg
//
LOCUS       DATMKH010000638         1031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2864052, whole genome shotgun sequence.
ACCESSION   DATMKH010000638 DATMKH010000000
VERSION     DATMKH010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2864052"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            732..956
                     /locus_tag="VNV63_07645"
     CDS             732..956
                     /locus_tag="VNV63_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016875794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HXC62532.1"
                     /translation="MDYRSIITIEPGKRSGKPCIRGLRITVYDVLGMLATGMSHSEIL
                     ADFPYLTEEDIKACLAYAADSDRRLLIVGS"
     gene            953..>1031
                     /locus_tag="VNV63_07650"
     CDS             953..>1031
                     /locus_tag="VNV63_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011142392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HXC62533.1"
                     /translation="MKLLFDENLSPKLVQLLADDFPGSVH"
BASE COUNT          243 a          289 c          259 g          240 t
ORIGIN      
        1 taaggacgtt ctctcttgag cgtttcagtc cagcgaccgg tggcggccga acgccgactg
       61 aaaggtacct aagctgcgcc ttgctcaata ggatcacgta ttacgaactg gatatcttgc
      121 ggggccgggt gcgcagcgta ctcaacagac ttggtaccga caatcacagc cgcctcgcaa
      181 caaaggaagt tctgcgatcg aacaccccca ctcttaccgc gaatggaaca tagtgggcca
      241 atgtgacgtg ccggggtttt tctatttgga atcccgattc cgcttgaacc ctggagagta
      301 ctaccgatcg ggaagaaaca agagattttg atcaactgga caagcttccc accacctttc
      361 gcttgcactt agtgaaagac gaggttcttg catacgcatg aaacttccgc gggacctcgc
      421 tgaccgtgcg atcatcaagg tccttgccct tgataaaaga agctgtgtcg atctggttcc
      481 cacttgacct ccaggggctg cccctgacct ttgacgcttt cctttgtgtc ggaagcgcgg
      541 tacccttgac ataaggccgc gccgtggaag ccaggattta taggagaaca tcggtcctcc
      601 tcggagaatc ggggagcgac gcatccgagg ggaactttct tttccaaaga aaagcccaca
      661 aagtcctcta gtcttaccgt tgtcaccgag tcgaaccgac tgttaaatat tctcaaaact
      721 acggcgaaac catggattat cgctcgatca tcacgattga accaggcaaa cgaagcggta
      781 aaccctgtat tcgtggcctg cgaatcacgg tgtatgatgt cttgggaatg cttgccaccg
      841 ggatgagcca ctccgaaatc ctggccgact ttccctatct caccgaagag gacatcaaag
      901 cttgccttgc ctacgccgcc gattcggata gacgcctgct catcgtcgga tcgtgaagct
      961 cctctttgac gagaatctct cgccgaagct tgttcagcta cttgccgatg acttcccagg
     1021 atcggttcat g
//
LOCUS       DATMKH010000639         1961 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1886198, whole genome shotgun sequence.
ACCESSION   DATMKH010000639 DATMKH010000000
VERSION     DATMKH010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1886198"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1320
                     /gene="glgA"
                     /locus_tag="VNV63_07655"
     CDS             <1..1320
                     /gene="glgA"
                     /locus_tag="VNV63_07655"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF001899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HXC62534.1"
                     /translation="KRWRLKSRGNLIVNLAGEVYSFTLWSGSLPGTPVEVLFLGNSAF
                     FNYSGLYQEKGKDYPDNLKRFSAFSRAVLEIPRQLNWYPDILHANDWQTALTIAYLKT
                     HLKEHAAYERMGTVFTIHNMGYPGLFPASEFPLLGLPSNYFTPDMLEFYGKVNLLKAG
                     LVLGGVLNTVSPTYSREIQSPEFGYGIEGILQTRRRDLFGIINGVDYQQWDPAHDPYL
                     PQSYDHDHLEGKRVCKLALQKECKLPVTEAPVIGMISRLTTQKGVDLILEMLDELMQL
                     DVQFVILGTGDPVIQRDLQEAMQRYPDKLSVHLTFDEALAHRIEAGADLFLMPSRYEP
                     CGLNQLYSLRYGTIPVVRKTGGLADTVVDAIPSNIASGKATGFMFEAASSHALLTTLR
                     LAFQFYNDRSLWKRMMRTAMKADFSWDQSAREYVELYEQALAKARGG"
     gene            complement(1338..>1961)
                     /locus_tag="VNV63_07660"
     CDS             complement(1338..>1961)
                     /locus_tag="VNV63_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein
                     domain-containing protein"
                     /protein_id="HXC62535.1"
                     /translation="HAGNFMFNRQRQIEYLSSTMDRPPLVVAPYDAELFGHWWFEGPE
                     WINMLIRKLACDQDTVELITPSDYLEDFPVNQESTPAGSSWGHKGYNEVWLNNRNDWV
                     WRHLHQAARRMEELIRNYPNSEGVLRRALNQASRELLLAQSSDWPFMMKTGQQVEYAV
                     KRFNDHLLRFLRLSDEIRSKQIDEIWLKELEDRDNLFPALDYRRFRF"
BASE COUNT          426 a          526 c          520 g          489 t
ORIGIN      
        1 aagcgatggc ggctcaaatc gcggggcaat ctcattgtga accttgccgg tgaagtctat
       61 tccttcaccc tctggtccgg cagtctgccc ggcaccccgg tggaggttct gttcctcggc
      121 aactccgcct tctttaacta ctccgggctt tatcaggaga aaggcaagga ttaccccgac
      181 aatcttaagc ggttctcggc cttcagccgc gcggtgcttg agattcccag acagttgaat
      241 tggtatccgg atatactgca cgccaatgac tggcagacgg ctctgacaat tgcatatctg
      301 aaaacccatt taaaagaaca tgcggcctac gaaagaatgg gcacggtttt taccatccat
      361 aatatgggct atcctggatt gttccctgct tcggagtttc cattattagg tcttccttcc
      421 aactacttta cgcctgacat gctcgagttc tatgggaaag tgaatctgct aaaggcggga
      481 ttagtgcttg gcggtgtctt aaatactgtg agccccacct atagtcgtga aattcagtct
      541 cccgagtttg gttacggcat cgaaggtatc cttcagaccc ggcggcgtga tctttttgga
      601 atcatcaatg gggtggatta ccagcagtgg gatccggccc atgatcccta cctcccgcag
      661 tcctatgacc acgatcatct tgaaggaaag cgcgtctgca agctggcgct ccaaaaggaa
      721 tgcaaactgc ccgtgaccga ggcacccgtc attggaatga tctcgcggct gacgacacag
      781 aaaggtgtgg atctgattct ggaaatgttg gatgagctga tgcagcttga cgttcaattc
      841 gttatcttgg gtaccggtga tcctgtcatt cagcgggatc ttcaggaggc gatgcagcgt
      901 tatccggata aactttcagt tcatctgacc tttgatgaag ccctggccca ccgcatcgag
      961 gccggggcgg atttgtttct catgccctcg cgctatgagc cctgcggact caaccagctc
     1021 tacagcctcc gttacgggac gattcctgtc gtgcggaaga caggaggctt ggccgatacg
     1081 gtggtagatg ccatcccttc caacattgct tcggggaagg ccaccggatt catgtttgaa
     1141 gcggccagca gccatgctct cctgactacg cttcgccttg ctttccagtt ttacaatgac
     1201 cgtagtttgt ggaaaagaat gatgcggacc gctatgaagg ccgacttctc ctgggatcaa
     1261 tcagcccgcg agtacgtcga gctttatgag caggccttgg caaaggcgcg gggaggttaa
     1321 ttattaatcg taatgaatta aaaacgaaac cgcctgtaat cgagcgcggg aaaaagattg
     1381 tcccggtctt ccagttcttt gagccagatc tcatcgatct gttttgatcg aatttcgtcc
     1441 gagagtctca aaaaacggag tagatggtca ttgaaccgtt tgaccgcgta ttccacctgt
     1501 tgacctgtct tcatcatgaa aggccaatcg gagctttggg ccaataacag ttctcgactg
     1561 gcttggttta aggcacggcg caggactcct tcgctgttcg ggtagttccg gatcagctcc
     1621 tccatcctcc gggcggcctg atgcagatgg cgccagaccc agtcgttccg gttattgagc
     1681 cagacctcat tgtacccttt gtgtccccaa ctcgacccgg ccggcgtgga ttcctgattc
     1741 accggaaagt cttccagata gtccgacggg gtaatcagct cgacggtgtc ctgatcgcag
     1801 gcgagtttgc ggatcagcat attgatccat tccggaccct caaaccacca gtggccgaac
     1861 agttccgcat cgtacggcgc cacgacgagc ggcggccggt ccattgtcga ggacaggtac
     1921 tcgatctgcc gctgccggtt gaacatgaag tttccggcat g
//
LOCUS       DATMKH010000640         1131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2864171, whole genome shotgun sequence.
ACCESSION   DATMKH010000640 DATMKH010000000
VERSION     DATMKH010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1131
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2864171"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /locus_tag="VNV63_07665"
     CDS             <1..918
                     /locus_tag="VNV63_07665"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HXC62536.1"
                     /translation="AEQAVDLAVVEVGLGGRFDATNTVSSLVSVITNIDYDHQQFLGK
                     RIEQIAYEKSGIIKPDVPVVTAALRPAALKVIQRECRYHRVPLYRLGKEFSVHNIHSP
                     FFDYQGIRQRLSELSCPLPGRHQFLNAALALASMELLDQQGWPVKPDAITHGLSKTIW
                     EGRLEVVRDEPLLILDGAHNPAGAAVLKEFLAESKSKRFERRVILVIGILKDKDIRAI
                     LRFLEPLADTVILTRPDDQRAAAPEDLLRRMPRSGKAVIFDRVPKAIREALDLARPED
                     LICVTGSLYTVGEAKAYLSGRGQPSRLRG"
     gene            920..>1131
                     /locus_tag="VNV63_07670"
     CDS             920..>1131
                     /locus_tag="VNV63_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62537.1"
                     /translation="MGLIRLLLLLVLFLAPLPIYAQEQAPRPAEKGRPVKPGQLPINL
                     TAERLEYFQGEEAYHADGSVELKKGS"
BASE COUNT          245 a          284 c          322 g          280 t
ORIGIN      
        1 gccgagcagg cggtggactt ggccgtggtg gaggtcggac tcggaggccg gtttgacgcc
       61 acgaatacgg tttcatccct ggtctcggtg atcacgaaca ttgattacga tcaccagcag
      121 tttcttggaa aaagaatcga gcagatcgct tatgagaagt ccggaatcat caagccggat
      181 gtcccggtgg tcacggctgc gttgaggccc gcggcattga aggtgataca gagagaatgc
      241 cggtatcatc gcgtaccgct ttatcgtcta ggaaaggaat tttcggttca taatattcat
      301 tctccttttt ttgattacca ggggattcgg caacgtcttt ctgagctttc ttgcccttta
      361 ccgggtcgtc accagttttt aaacgcggcc ctggccctgg cttcgatgga acttctcgat
      421 cagcagggct ggccggtaaa accggatgcg atcactcacg gtctttccaa gacaatctgg
      481 gaggggaggc tggaggtggt cagagacgag cctcttttga tcctggacgg cgcccataat
      541 ccagcgggcg cagcggtttt aaaagagttt cttgctgaat cgaagagcaa acgtttcgag
      601 agacgtgtga tcctggttat tggaatcttg aaggacaaag atatccgtgc gatcctaaga
      661 ttcttagagc ctttggcaga taccgtgatc ctgacacgac cggatgatca acgtgctgct
      721 gcgcccgagg atcttttaag acggatgccc cgatccggga aggccgttat ttttgatcga
      781 gtccctaagg cgatccggga ggcccttgac ttggcccgac cggaggatct tatctgcgtg
      841 accggttctc tctatacggt cggtgaggca aaggcgtatc tttccggtcg aggtcaacct
      901 tcccgtctca ggggatgaga tgggtctgat ccgtcttctc ttactccttg ttttatttct
      961 tgcacccctc ccgatctatg cacaggaaca agcgcctcga cctgcggaga aaggccgtcc
     1021 ggtaaagccc ggacagcttc cgatcaatct cacagccgaa cggctggaat attttcaggg
     1081 agaagaggcc taccatgcgg acggatcagt cgagctcaag aagggttcat t
//
LOCUS       DATMKH010000641         1752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2525154, whole genome shotgun sequence.
ACCESSION   DATMKH010000641 DATMKH010000000
VERSION     DATMKH010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2525154"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VNV63_07675"
     CDS             complement(<1..221)
                     /locus_tag="VNV63_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HXC62538.1"
                     /translation="METAPSSPATQTQDLKARVQQVLNTIRPALQMDGGDCDLVDVTA
                     DGIVHLKLRGACGSCPSSTMTLKMGIESRL"
     gene            complement(267..1019)
                     /locus_tag="VNV63_07680"
     CDS             complement(267..1019)
                     /locus_tag="VNV63_07680"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HXC62539.1"
                     /translation="MKSPLIIAHRGASGDAPENTMAAFRLAIDSGADMIELDIHQSKD
                     GALVVCHDFKLYRTARSKKTVKQLTLKELKKLDVGHWFHPSFSGETIPTLEEVLKLTA
                     TRIRLNIEIKQGSSLYPGIEKRLVELLESSRMLESVMVSSFDDHALDAVRALNPEISI
                     GLLAERGDIKHLIRKAAGLSASSLNLYTQLVTPGLVQEAHRHKLQVHVYTVNQVRLMN
                     YYLLTGVDGVFTNYPARLAQLFKRQKQLAKIG"
     gene            complement(1024..>1752)
                     /locus_tag="VNV63_07685"
     CDS             complement(1024..>1752)
                     /locus_tag="VNV63_07685"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HXC62540.1"
                     /translation="ECMVLLRGEKTQHYQGNLLLDRLLGADVIFVDPEEYARIDAAAE
                     RFAEKLGRQGKKAYLIPEGGSNVLGSLGYVQMVKELAGQMKKDRLTYDSIVCAIGSGG
                     TYAGLLLGKKLFKLKSEIIGFNVCQDAAYFKKKIMAIIEQAIERYHLPVRVHPDDIKI
                     IDGYVGPGYALSSPQQLTTLREVAQLEGVVLDPVYTGKTMHGLLDQLRQGTKQLGRRI
                     LFIHTGGIFGLFPKAEELSEVVIG"
BASE COUNT          394 a          476 c          457 g          425 t
ORIGIN      
        1 aggcggctct cgatgcccat cttcaaggtc atcgtggaag agggacagct gccgcaagcc
       61 ccccggagtt ttaaatgaac gataccatct gcggttacat ccaccaggtc acaatcgcct
      121 ccgtccatct gtagcgcggg acggatcgta ttaagaacct gctggactct tgcttttaag
      181 tcttgcgttt gtgttgctgg gctgctggga gcggtttcca taacctctaa acctcctcat
      241 gaaatgtata agtagaatag cacgacctaa ccgatcttcg caagctgttt ttgccgcttg
      301 aagagctgag ccagccgagc cgggtaattg gtaaagaccc catccacacc cgtcagcaga
      361 taataattca tcaaacgaac ctgattgacg gtgtagacat gcacctgcag tttgtggcga
      421 tgagcctcct gtacgagacc gggcgtcaca agctgtgtat aaaggtttaa agatgaagcg
      481 gacaggccgg cggcttttcg gatgagatgc ttaatgtcgc ctcgttccgc cagcaggccg
      541 atggagattt caggatttag ggcccggacc gcgtccaagg catgatcatc gaaagaagaa
      601 accatcaccg attcaagcat tcgggaagat tccagcaatt caaccaagcg tttttcgata
      661 cccggataca gggacgaccc ttgcttgatt tcgatattaa gccggatgcg tgttgccgtt
      721 aactttaaaa cctcttccag tgtcggaatc gtctcacccg aaaaacttgg atggaaccaa
      781 tgaccgacat ccagtttttt taattccttg agcgtaagct gcttgacggt tttcttggag
      841 cgtgcggtgc ggtaaagttt gaaatcatgg cagaccacta gcgcgccgtc tttgctctgg
      901 tgtatatcga gctcgatcat gtccgccccg gaatcgatgg ccaggcgaaa agcggccatg
      961 gtattctccg gagcgtcgcc ggatgccccg cggtgggcga tgataagagg gcttttcatg
     1021 ctgctatccg atcacgacct cgctcagttc ctcagctttc gggaacaggc cgaagattcc
     1081 gccggtgtgt atgaacagta tgcgccggcc aagttgtttc gtgccttggc ggagctggtc
     1141 cagaagacca tgcatggttt ttccggtgta gaccggatcc aggacgacgc cttccagttg
     1201 tgcgacctcc cttagcgtgg tgagctgttg cggcgaagag agggcatacc ccggaccgac
     1261 atagccgtct atgattttga tatcatccgg atgaacgcgg accggcagat gatagcgctc
     1321 gatcgcctgt tcgatgatcg ccatgatctt ctttttaaaa taggccgcat cctggcagac
     1381 attaaagcca atgatctctg atttaagttt aaacagtttt ttgccgagca ggaggcccgc
     1441 ataagtcccg ccggagccga tggcgcagac gatactgtca taggtcaacc gatccttctt
     1501 catctggcct gccagttcct tcaccatctg cacgtatcct aaagacccca ggacgttgga
     1561 gcctccctcc ggtatgaggt aggccttctt tccctgccgg cccaattttt cggcgaaccg
     1621 ttccgcggcc gcgtcaatgc gcgcatactc ttccggatcc acaaagatca catccgcccc
     1681 gagcagacga tctaacagga gattgccctg gtaatgctgt gtcttctctc cccgaaggag
     1741 taccatgcat tc
//
LOCUS       DATMKH010000642         1049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2864608, whole genome shotgun sequence.
ACCESSION   DATMKH010000642 DATMKH010000000
VERSION     DATMKH010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2864608"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..931)
                     /locus_tag="VNV63_07690"
     CDS             complement(<1..931)
                     /locus_tag="VNV63_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62541.1"
                     /translation="MKAGRRMGLAFTTGDQGANGEMRNPSWSPDGKRVVYQKWFSESW
                     RQNQPLFSIDPEFDLAYSDPFPAFSHDGKKLAVTHSVSNRGTGSSSLLSVAVMNADGR
                     NAKLVFHGEEGGVALAPQWSPNDEWIAFGGGFYFLPRGRPARVMTMRSDGSELHELTK
                     TAGNSGFPSWSPDGKRMVFRFWGQGEYGLRIINLDDGSSTKLTTGFDNFPAWSPKADL
                     IAFTRFSEDDYDIYTIRPDGTGLRRLTTARGNDAHAVWSPDGKHILFSSARLGFKDEA
                     PLYDGIPQPYGELFVMDADGSNQRPLTDNQWEDA"
BASE COUNT          237 a          305 c          267 g          240 t
ORIGIN      
        1 ttgcatcttc ccactggtta tcggtcagcg gtcgttgatt ggagccatcc gcgtccatca
       61 caaagagttc accataaggc tgaggaatgc cgtcgtagag cggtgcctca tctttgaatc
      121 cgagacgggc gctgctgaag agaatatgtt taccatctgg agaccataca gcgtgtgcat
      181 catttcccct agcagtggtc agtctcctta atccggtccc gtcgggccgg atcgtgtaaa
      241 tgtcatagtc gtcttcacta aagcgggtga aggcgataag atcggctttt ggagaccagg
      301 caggaaaatt gtcaaatccg gtggtcagct ttgttgagga tccatcgtcc agattgatga
      361 ttcggagacc atactccccc tggccccaaa agcgaaacac cattcgcttg ccatccgggg
      421 accaactcgg aaacccactg tttcccgcag tcttggttag ctcatggagt tccgaaccat
      481 ccgagcgcat cgtcataacg cgggccggcc tgccgcgagg gagaaagtag aaacctcctc
      541 caaacgcgat ccactcatcg ttaggggacc attgcggcgc cagggccaca ccgccctcct
      601 ccccatgaaa gacgagtttc gcattcctcc catccgcatt catcaccgcg actgaaagga
      661 gcgagctgga gcccgtccca cgattactca cgctatgagt cacggcaagt ttcttgccgt
      721 cgtgcgaaaa cgcgggaaag gggtccgagt aagccagatc aaattccgga tcgatactga
      781 acaagggttg gttttgacgc catgattccg agaaccattt ctgatacaca acccttttgc
      841 catcaggtga ccaagacgga ttgcgcatct ctccattcgc cccttgatct ccggtcgtga
      901 aggctaaacc catccgtcgg cctgccttca tgacatatcc gattctattc ccgctaagaa
      961 actgcggtga aactttcagc cccggacctg acgtgtgctc ctggcgagtt ccggttgcta
     1021 cctgttgcat gaccgggatg cgaagttct
//
LOCUS       DATMKH010000643         1752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1580151, whole genome shotgun sequence.
ACCESSION   DATMKH010000643 DATMKH010000000
VERSION     DATMKH010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1580151"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1186
                     /locus_tag="VNV63_07695"
     CDS             <1..1186
                     /locus_tag="VNV63_07695"
                     /inference="COORDINATES: protein motif:HMM:NF012975.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HXC62542.1"
                     /translation="LTAEMVFDSQGQPLDYQLYESVIRSEERMTYTALKEILVDRDPA
                     VCQRYQALLEDFRLMEVLCLKLRHRRLAMGSIDFDLPEPQILLDVLGQPTAILREERT
                     IAHQIIEEFMLVANQTVAGHLTRLGLPTIYRIHEPPDPKKIEAFRLFVQGLSGMILRS
                     PKGEIQKTDNTMRPKSFQTLLEHAKGSPHERLINHLLLRSMKQARYSTQNLGHFGLAF
                     DCYTHFTSPIRRYPDLVVHRILKEALKATLHRGKTRGDLEPIAEHASERERLSMEAER
                     EAVKLKKVRFMRDKPGEEFSGFITGVTAFGLFIELEEIFVEGLVPITSLQDDDYLYLQ
                     DKHCLLGKRHRRMLRVADPVKVRVERVDLEKRQVEFALLQARGKSLPAPSLKRYSGRR
                     RR"
     gene            1193..1363
                     /locus_tag="VNV63_07700"
     CDS             1193..1363
                     /locus_tag="VNV63_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62543.1"
                     /translation="MAQKAKTKVEKKKTCMICGKPSPDTICESCKIKIQAEAIEKKQE
                     IEKEGRTDTSRR"
     gene            1531..>1752
                     /locus_tag="VNV63_07705"
     CDS             1531..>1752
                     /locus_tag="VNV63_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011403652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXC62544.1"
                     /translation="MRLKKLELLGFKSFCDKTTITFQPGITSIVGPNGGGKSNLVDAI
                     LWVTGEQRTKVLRSEQMEDVIFNGSDTRKP"
BASE COUNT          440 a          445 c          483 g          384 t
ORIGIN      
        1 actcacagcc gagatggtgt tcgattcaca ggggcagccg cttgattatc agctctatga
       61 gagcgtgatt cgaagcgagg agcggatgac ctacaccgcg ctcaaggaga tcctggtgga
      121 ccgtgatccc gccgtctgcc aacgttatca ggctttgctc gaggatttcc gcctgatgga
      181 ggtgctctgc ctgaagcttc gacatcgtcg gctggccatg ggcagcattg attttgacct
      241 gccggaaccg cagatactgt tggatgtcct gggacagcca accgcgattc tccgggagga
      301 acggacgatc gcacatcaaa tcattgaaga gtttatgctg gttgccaacc agaccgtggc
      361 aggacacctg acccggctcg gtctccctac gatctatcgg atacatgagc ctccggatcc
      421 gaagaagatc gaggcgtttc ggctatttgt tcagggcctg tctggtatga tcctgcgatc
      481 gccgaaaggc gagatacaaa aaacggacaa taccatgcgt ccgaagtctt tccagacact
      541 cctcgagcat gccaagggga gtccgcatga gcggctgatc aaccacctct tgctccgttc
      601 gatgaaacag gcccgctact cgacacagaa cctgggacat ttcggcctgg cttttgactg
      661 ctatacccat ttcacatccc ccattcggcg ttatccggat cttgtcgttc accgaatcct
      721 aaaggaggcg cttaaagcaa ctcttcatcg agggaagaca cggggcgatc tggagccgat
      781 tgcagagcat gcctcagaac gggaacgcct ttcaatggaa gcggagcggg aggcggtcaa
      841 actcaaaaaa gtgcggttca tgagggacaa gcccggtgaa gagttttccg gtttcatcac
      901 gggcgtgacc gcgttcggcc tcttcataga gctggaagaa atctttgtag aggggctggt
      961 cccgatcacg agtctgcagg atgatgatta tctctatctc caagacaagc attgtctctt
     1021 gggaaagagg catcgaagga tgctccgagt tgcggatccc gtcaaagtac gtgtggaacg
     1081 ggttgatttg gaaaaacggc aggtggagtt tgcattgctt caggcgcggg gaaaaagtct
     1141 tccggcccca tcgcttaaaa gatattcagg gagacgaagg aggtaaactt cgatggcgca
     1201 aaaagcgaag actaaggtag aaaaaaagaa gacctgcatg atctgcggca aaccatcgcc
     1261 tgatactatc tgtgagtcct gcaagattaa gatccaggcc gaggcgatcg aaaagaaaca
     1321 ggagatcgaa aaagagggcc gcaccgatac cagccggcga tgaccggcct ttatcaacgc
     1381 atacgacctg ttgtagtggc ctaaccggtt aacatacacg atcttgtatt ttccgcttta
     1441 catcattata ttgttatggt aaggttctct cttgaaacag gaccctgatc cttatctcgc
     1501 caaagggtcc tccgttcaaa tgggagggcg atgcggctta agaaattaga actcctcggc
     1561 tttaaatctt tctgcgataa gactactatc actttccaac ccggtattac ctcgattgtc
     1621 ggcccgaatg gcggagggaa gagcaacctt gtagatgcca tcctgtgggt tacgggcgag
     1681 cagcgcacaa aggtcctccg aagcgagcag atggaggacg tgatcttcaa cggctccgac
     1741 acccgaaagc cg
//
LOCUS       DATMKH010000644         1325 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4759840, whole genome shotgun sequence.
ACCESSION   DATMKH010000644 DATMKH010000000
VERSION     DATMKH010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4759840"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..986
                     /locus_tag="VNV63_07710"
     CDS             <1..986
                     /locus_tag="VNV63_07710"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HXC62545.1"
                     /translation="VFGVSLMLILPRVSALRGVAVAVAMLIGYMLLVQYLFEMKGLWI
                     TLLYPAATIAFSYTSLTVLRFMTEERQAQEVRRMFSSYVTPKMVAELVKDPDKAKLGG
                     ERKELTVLFSDVRGFTTFSENHQPEEVVAILNEYMNAMTEVIFHWDGTLDKFVGDAIM
                     VFWGAPLDQPNHAELAIRCALHMQKRLGELQEKWRADGKETLDAGIGMNSGEMVVGNM
                     GAEGKKMDYTVIGDNVNLAARVESLTRQYNARILITESTYHGAKDLIEVREIFANEKR
                     SRATGIKHKERRRKRMKIGHALFKDLDAVKVKGKVKPVRIFEVVGIEEERK"
     gene            983..>1325
                     /locus_tag="VNV63_07715"
     CDS             983..>1325
                     /locus_tag="VNV63_07715"
                     /inference="COORDINATES: protein motif:HMM:NF014199.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXC62546.1"
                     /translation="MRLRVLGCYGAELSNHGTCGFLINRSVLVDGGTICSSLSLSEQR
                     GIRYILISHVHADHIKGLPFLSENLIGQKGQKPIVVIGIGEVLAGLRQHLFNNQIWPD
                     FTKLPNVRNPIF"
BASE COUNT          316 a          310 c          400 g          299 t
ORIGIN      
        1 tggtattcgg cgtgagcctg atgctgatcc ttcccagggt cagcgcgctt cgcggcgtgg
       61 ccgttgccgt cgcgatgctc atcggctaca tgcttctggt ccagtatctc ttcgagatga
      121 aaggcctctg gattacttta ctctatcccg ccgccacgat tgccttttct tacacatcgc
      181 ttacggtgct ccggttcatg acggaagaac ggcaggcgca agaggtccgg cggatgttct
      241 caagttatgt gacccctaag atggtcgccg aactggtgaa ggatccggat aaagcgaagc
      301 tgggaggaga gcgaaaagag ctgaccgttt tgttttcgga tgtgcgcggc tttaccacct
      361 tttcggaaaa tcatcagccg gaagaggtgg tggccatctt aaacgagtac atgaacgcca
      421 tgaccgaagt gatctttcac tgggacggga cgctggataa gtttgtggga gacgcgatca
      481 tggttttctg gggcgctcca ctggaccaac ccaaccacgc cgagttggcg atccgctgtg
      541 cactgcatat gcaaaaacgt ctgggcgagc ttcaggagaa gtggcgcgca gatgggaagg
      601 agacactgga cgcggggatc gggatgaata gcggggaaat ggttgtggga aatatggggg
      661 cggaagggaa aaaaatggac tataccgtga tcggagacaa cgtcaacctg gctgcccgtg
      721 tggaatccct gacccgccaa tataacgccc gtatcttgat cacggagtca acctatcatg
      781 gggccaaaga tctcatcgag gtcagggaga ttttcgccaa tgagaagcga agcagagcca
      841 ccggaattaa acacaaggag cgaaggagaa aacggatgaa gattggacat gccctgttca
      901 aggatctgga tgcggttaag gtcaaaggga aggtcaaacc tgtccgtatt tttgaggtgg
      961 tcggaataga agaggagcgg aaatgagact gagagtgctg ggatgttacg gcgcggaact
     1021 ttcgaatcac ggcacctgcg ggtttctgat taatcgttcc gttttagtgg atgggggaac
     1081 catttgttct tccctgagcc tctctgagca gcggggcatc cgctatatac tgatctcgca
     1141 cgtccatgcg gatcacatca aaggtctccc gtttttatcc gagaatctca tcggccagaa
     1201 ggggcaaaag ccgatcgtcg tgatcggtat cggggaggtg ctggccgggc ttcgacaaca
     1261 cctttttaat aaccagattt ggccggattt tacgaagctg ccgaatgtcc ggaaccccat
     1321 ttttc
//
LOCUS       DATMKH010000645         1239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3170259, whole genome shotgun sequence.
ACCESSION   DATMKH010000645 DATMKH010000000
VERSION     DATMKH010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1239
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3170259"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1239
                     /locus_tag="VNV63_07720"
     CDS             <1..>1239
                     /locus_tag="VNV63_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62547.1"
                     /translation="PPRGEKPNPKPKEQEKPNNVISSMIIDEVTCKNTVLITLPRKPD
                     PGKPQKEPLEWDIHDLDFTSASLDKPFHFHGKLTNAKPKGEIDTTGDFGPWNLDEPSA
                     TPVSGTYKFTDADLGPFPGIAGILSSTGKYNGEIAELQVEGETDTPDFSLDKIGKPVP
                     LHTEYSATVDGTNGDTLLHPVRATLIRSLIIAEGSVINEPGHIGHRIVLNIRAPNARI
                     QDILSLAINSDKPILSGPAKIKAKLILPPGKEKVLSKMILDGQIGIDDARWTSAEVRG
                     KLESLSRHGEGKPSDDDAGSAVSDLRGRFHLEKGIITFSNLTFGVPGATIDLAGTYQI
                     QGGQIDLNGHLRLQAKLSQTVTGTKSFFLKALDPFFAKEGAGAVIPISISGTRDHPTV
                     GVTILHKKIEKKMGDTKSAL"
BASE COUNT          330 a          362 c          305 g          242 t
ORIGIN      
        1 tccgcctcgg ggagaaaaac cgaatccgaa acccaaagag caggaaaaac caaacaacgt
       61 gatctcttcc atgatcatcg atgaggtcac ttgcaagaac acggttctta ttacgctccc
      121 gagaaagccg gatccgggaa agcctcaaaa agaaccgctg gaatgggaca ttcacgatct
      181 tgatttcact tccgcaagtc tggacaagcc ctttcatttc catgggaaac tgacgaacgc
      241 gaagcccaaa ggagaaatcg acaccaccgg cgacttcggt ccgtggaatc tcgatgagcc
      301 cagcgccacg cctgtttccg gtacctacaa attcaccgac gccgatcttg ggcccttccc
      361 cgggatcgcg ggcattttgt cttctacagg aaagtacaac ggcgaaatag cggagcttca
      421 agtggaaggc gaaacagaca caccggattt ctctcttgac aaaattggca agccggtacc
      481 gctccacacc gaatactccg ccacggtcga cggcaccaac ggagatactc tgctccaccc
      541 cgtgcgcgct accctgatcc gctcgcttat cattgcggaa ggcagcgtca tcaacgagcc
      601 cggccatata gggcacagga tcgtcctcaa catccgcgcg cctaacgccc gcatccagga
      661 tatcctcagt ctcgctatta attccgacaa acccatcttg agcggccctg caaagatcaa
      721 ggcaaaatta attctgcctc ctgggaaaga aaaagttctg agtaagatga ttctcgatgg
      781 ccagattggt attgacgatg cgcggtggac cagcgcggaa gtccgaggaa aacttgagtc
      841 cctgagccgc cacggtgaag gcaagccgag cgacgacgat gcgggaagcg cggtctccga
      901 tctccgaggc agattccatc tggaaaaagg cattatcact ttttccaatt tgacgttcgg
      961 cgttcccggg gcgaccatcg acctcgccgg cacttatcaa atccagggag gccaaatcga
     1021 tctcaacgga caccttcgcc ttcaggcaaa gctctctcaa accgtcacag ggacaaaatc
     1081 tttttttctg aaggctcttg atccgttctt cgcgaaagag ggggccggag cagttattcc
     1141 catctcaatt tccggcacac gagaccatcc gacggtcggc gtcacgatct tgcacaagaa
     1201 aatcgagaaa aagatgggcg ataccaaatc ggcgttgca
//
LOCUS       DATMKH010000646         1092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2527024, whole genome shotgun sequence.
ACCESSION   DATMKH010000646 DATMKH010000000
VERSION     DATMKH010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1092
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2527024"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1092
                     /locus_tag="VNV63_07725"
     CDS             <1..>1092
                     /locus_tag="VNV63_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62548.1"
                     /translation="EDIYGVGVVEEMERDEVLIDRILNMDLRQLLLCINPPGFYSGTE
                     DFENDNIKLRAGVVRRTLDPQGINFLKIPPPDKAALEMIQYIEDKEDSRTGINPLVEG
                     EIPVQSGGKGSKATEFLVARESSLRKLQLPKHSLEDALGWEFMNRIALIQQVYSDFEV
                     EHLEDETEIQDYLQSVNADRSFFFIENEGVAGKEKFYVKRFREMPLSVEQDESGQFLE
                     SEDTSFFKIKPEFLPWEGDIIIDTNAMLSNSDQLERQNTLQLAEILDPLFQGDPKINL
                     KIAKQILLSFKKDPEKWFPADWVEFLRDPSKMTQQQQPQGALGAPGGPGGPTGGQPQP
                     QGGGGKPAPKQSNTGGIRGMMKKIGAGLGI"
BASE COUNT          291 a          263 c          320 g          218 t
ORIGIN      
        1 gaagatatct atggcgtggg agtcgtggaa gaaatggagc gcgacgaagt gttgattgac
       61 cggattttga acatggactt gcgacagttg ctcttgtgca tcaatcctcc aggtttttat
      121 tccggcaccg aggacttcga gaacgacaat atcaagctcc gtgccggtgt ggttcgccga
      181 acgctcgacc cgcagggcat aaacttcctg aagatcccgc cgccggacaa ggcagctttg
      241 gagatgatcc agtacatcga agacaaggag gattcccgta ccggcatcaa tccgcttgtc
      301 gagggagaga tccctgttca gtcgggaggc aagggatcga aagcaaccga gtttctcgtt
      361 gcgcgcgaat catcgttgcg gaagctccaa ttgccaaaac actctcttga ggacgcgtta
      421 ggatgggagt tcatgaaccg catcgctttg atacaacaag tgtattccga cttcgaagtc
      481 gagcatttgg aagacgaaac cgagatacag gattacttgc aatcggtgaa cgccgaccgg
      541 tcgttcttct tcatcgagaa cgagggtgtg gccggcaagg agaagttcta cgtcaaacga
      601 ttcagagaga tgccgttgtc cgtcgagcag gatgagtcgg ggcagttcct cgaatccgaa
      661 gacacgtcat tcttcaaaat caagccggag tttttgccgt gggaagggga tatcatcatt
      721 gacacgaacg cgatgctctc gaactccgat caattggagc ggcagaacac gttgcagctg
      781 gcggagattc ttgatccgtt attccaaggc gacccgaaga tcaatctgaa aatagcgaaa
      841 cagattttgc tttcattcaa gaaagaccct gagaagtggt tcccagcgga ttgggtagag
      901 tttctccgcg acccatcgaa gatgactcaa cagcaacagc cgcaaggcgc actaggagca
      961 ccaggtggcc ccggagggcc aacaggaggg caaccgcagc cacagggcgg aggaggcaaa
     1021 ccagcgccaa aacagagcaa tacgggcggt atcaggggca tgatgaagaa gataggggcg
     1081 ggactcggca ta
//
LOCUS       DATMKH010000647         1174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_939043,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000647 DATMKH010000000
VERSION     DATMKH010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_939043"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="VNV63_07730"
     CDS             <1..493
                     /locus_tag="VNV63_07730"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HXC62549.1"
                     /translation="LTFMVGGEETVFEKVRPWLEIMGKKLYFCGGPAMGIHAKLTQNL
                     VLSNLLQAFNEGIVLSTKAGVDPKLMLDILSNSAAKSGLIDYKAPFIFRRDFATNFSV
                     KWMHKDMGLMLESAQELSVPLFLTSLSRQLFQSAITRGHGEEDICATIKVLEEMTGVE
                     VKS"
     gene            757..>1174
                     /locus_tag="VNV63_07735"
     CDS             757..>1174
                     /locus_tag="VNV63_07735"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:NF014700.4"
                     /note="catalyzes the formation of 2-C-methyl-D-erythritol
                     4-phosphate from 1-deoxy-D-xylulose-5-phosphate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HXC62550.1"
                     /translation="MPTRTLTILGSTGSIGTNTLDVVGQNSDRFRVFALAAGRNIDLL
                     AQQIQRFKPAVVVLERAEDVDSLRALLSDSEIPELLHGPDALTRIAVAPEIDTVMSSI
                     VGVAGLPATYEAICAGKRVGLANKEVLVSGGKMVMDA"
BASE COUNT          290 a          312 c          314 g          258 t
ORIGIN      
        1 cctcactttc atggtcggcg gcgaggaaac cgtttttgaa aaagtccgcc cctggcttga
       61 gatcatgggg aagaagctct acttttgcgg tggacccgcc atgggcatcc acgccaagtt
      121 aacccagaac ctggtactct ccaatctgct ccaggcgttc aacgaaggca tcgtcctcag
      181 cacgaaggcc ggggtcgatc cgaagctgat gctcgacatc ctttccaaca gcgccgcaaa
      241 atccggcttg atcgactaca aagctccctt tatattccgc cgggatttcg cgacaaattt
      301 ctccgtaaaa tggatgcaca aggatatggg actcatgttg gaatcggccc aggagctttc
      361 cgtacctctt ttcctgacca gtttgagtcg gcaacttttt cagtcggcta tcaccagagg
      421 gcacggagag gaagatatct gcgctacaat caaggtttta gaggaaatga ccggagtaga
      481 ggtcaaaagc tgacgtgcgg aatcggacca atataaaatt tatatacaaa atttggttga
      541 ttccaaaaac aatctattct attattggaa tcaagccggg gaggaaaagt aactcccacc
      601 aaagcgagac taacctctaa actgaaagac ccctgaagca acctccccgg cgcccgcaag
      661 ggtcagtact aaaaccggct gcttccagag acaaagggtt ctgaagtctt cacaaaataa
      721 gccatgatat gatcggggaa gatcgtaacg gaaccgatgc ccacccgtac gcttacaatc
      781 cttggttcca ccgggtcgat cggcacgaat acgctggacg tcgtcggcca gaattcagat
      841 cgatttcgcg tttttgcgct ggcagccgga cggaacatcg atcttctggc tcagcagatt
      901 caacgattta agcctgcggt agtggtcttg gaacgcgccg aggatgtgga cagtctgcga
      961 gcgctccttt cggactctga gatcccggaa ctcctgcacg gccctgatgc cttgacccgg
     1021 atcgccgtcg cacctgagat cgacacggtc atgtcgtcga tcgtgggggt tgccggcctt
     1081 cccgcgacct acgaagctat ttgcgcgggc aagcgcgtgg ggctggctaa caaagaggtg
     1141 ttggtttccg ggggcaaaat ggtgatggac gcgg
//
LOCUS       DATMKH010000648         2467 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3808142, whole genome shotgun sequence.
ACCESSION   DATMKH010000648 DATMKH010000000
VERSION     DATMKH010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3808142"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..898
                     /locus_tag="VNV63_07740"
     CDS             <1..898
                     /locus_tag="VNV63_07740"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HXC62551.1"
                     /translation="KALDDLNFPIDLVCGTSVGALIAAMIVQGDVKRLVDIWWNLGAE
                     QVYKQNSKLKFLFSFQMKYIYPYFSSEPLEKLIVDNIDPEKIRRSDKKLIVTACNQTL
                     RRLVRFTNTFENLPLALLASASIPIAYPPVLMNGFEFVDAGVIDNVPLKPAIEEGADK
                     IIVVVGSHPTDLPPPIKNNLEWALWLIDIAHAHNVFADIEMAKKINFSEDREKKGYKL
                     IDLTLMAPPQGLDLNVTDFHKRNLIRTAIKLGYEDGMRAFNPRLRSGEVGLENIMKTT
                     FKEEGGGRDKDISYYWNESGQV"
     gene            1064..2356
                     /locus_tag="VNV63_07745"
     CDS             1064..2356
                     /locus_tag="VNV63_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018047942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HXC62552.1"
                     /translation="MQITVRSFLLPLILLSIILPSWAEGSQVDVVTYDGVINPVAAEF
                     LTKAIAIATEDRAEALIIQLDTPGGLDTSMRIIIKEISASAIPIVLYVSPPGARAASA
                     GVFITLASHIAAMAPGTNIGAAHPVAMGGGEMDKEMKKKVENDAAAYIKSITEKRGRN
                     VSWAEDAVRKSVSITEKDAVKLKVVDLIAENLPALLKAIDGRTVETAKGKVTLATKNA
                     TIKTIPMSFRFKVLNTLSDPNIAYILMLLGIYGLIFELSNPGSILPGIVGGISLILAF
                     YAFQTLPINYAGFLLILLAVILFIAEIKVPSYGLLTVGGVISMVLGSLMLITTDVPGL
                     QISMFVILPTALLTAGFFVLVVGMAWRAHQKKPVTGVEALIGMAGIAKTEIDPRGSVL
                     LQGELWEALSNEPIKKGEPVIVRGLDGLTLLVKRSKKT"
BASE COUNT          596 a          608 c          650 g          613 t
ORIGIN      
        1 caaggcattg gatgacctca atttccctat agatcttgtt tgcggtacaa gtgtaggggc
       61 cttgattgct gcaatgatcg tacaaggcga tgtgaaacga ttggtggaca tctggtggaa
      121 tctcggcgcg gaacaggtct ataagcaaaa ctcgaagcta aaatttcttt tctcctttca
      181 gatgaaatat atctatccct acttttcttc agagccgctt gagaagctca tcgttgacaa
      241 tattgatccg gagaaaatac ggaggagtga taaaaagctg atcgtcacgg cctgtaatca
      301 gacattacgc cgtttggtga ggttcaccaa tacctttgag aacctccctc tagctcttct
      361 ggcatcggcg tccattccaa tcgcctatcc gcctgtcctg atgaacgggt tcgagtttgt
      421 ggacgcgggg gtcattgata atgttccttt aaaacctgct attgaggaag gagccgataa
      481 gattattgta gtcgtaggca gccatcctac ggatctgccg ccgccgatca agaacaacct
      541 tgaatgggcc ctctggttga ttgatatcgc ccatgcacat aatgttttcg ctgatattga
      601 aatggcgaag aagatcaatt tttctgaaga cagggagaaa aaggggtaca aacttatcga
      661 cttgacgctc atggctccgc cacaagggct tgatctgaat gtgaccgatt ttcataagcg
      721 gaatttaatc cgaacggcga tcaagctggg ttacgaggat ggaatgaggg cgtttaatcc
      781 gagactcaga agcggggagg ttgggctaga gaacatcatg aaaacgacct ttaaggagga
      841 gggtggcggg cgcgacaagg atatatctta ctactggaac gaatcgggac aggtttaaac
      901 cgcacgtatc aggcgtttta aattccagaa aaccattatc aaccggtgta ccaaaaaact
      961 gaccacccaa tctgagtaac aggaccttta gagtccacca cccggcttga cagtccctcg
     1021 ttaaaagaat atgataactt tacctaaccg acgaggttct gccttgcaaa tcacggtgcg
     1081 ttcttttctc ttgcccctca ttcttctctc gatcatcctt ccctcctggg ccgaaggaag
     1141 ccaggtggat gtggttacct atgacggagt gatcaacccg gttgcagccg agttcctgac
     1201 caaggcgatt gcgatcgcga ctgaggatcg tgccgaggcg cttatcattc agcttgatac
     1261 cccgggcggt cttgacacct cgatgcggat cattattaaa gaaatcagtg cctcggcgat
     1321 cccgattgtc ctgtacgtct ctccacccgg ggcgcgtgcc gcctcagccg gtgtctttat
     1381 cacgctggcc tcacacattg cggcaatggc gcccggaact aatatcggcg cggcacatcc
     1441 ggttgcgatg ggcggcggcg agatggacaa ggagatgaag aagaaggtgg agaatgatgc
     1501 cgcggcttac atcaagtcta ttaccgagaa acgcgggcgc aacgtctcct gggccgagga
     1561 cgcagttcga aagagcgtct cgattacgga aaaggacgcc gtaaaactca aggtcgttga
     1621 cctgattgcg gaaaaccttc cggccctttt gaaggcgatt gacggccgga cggtggaaac
     1681 tgccaagggc aaagtgacgc ttgcaacgaa gaatgccacg atcaagacca tcccgatgag
     1741 ctttcggttt aaggtcttaa acaccttaag cgatccgaac atcgcctata tcctgatgct
     1801 tctcggaatc tacggcctga tttttgaact ctcaaatccg ggctcgatcc tgcctggtat
     1861 cgtcggaggg atcagcctga tcctggcctt ctacgcattc cagactctgc cgatcaacta
     1921 tgccggattt ctcctaattc tgcttgcggt tattttattc atcgccgaga tcaaggtccc
     1981 gagttacggc ttgctgaccg tgggcggtgt gatctcgatg gtccttggct ccttgatgct
     2041 gatcacgacc gacgtaccgg gtcttcagat ttcaatgttc gtgatcctgc ccaccgcact
     2101 cctcaccgcc ggtttctttg ttctggtggt cggcatggcc tggcgggcac atcagaagaa
     2161 accggtcacc ggtgtcgaag cgctcatcgg catggccggc attgccaaga cagagatcga
     2221 tcctcgaggc tcggtactgt tgcagggtga actctgggag gcactcagca atgaaccgat
     2281 taaaaaaggg gagccggtta ttgttcgagg gctggacggt ttgacgctat tagtgaaacg
     2341 ctcgaaaaaa acttagtacg gggggaacca tgtctcaact actctttagt ctgccgggtg
     2401 tcgttattat tgtatttctc ttcttgttca tctcgatcaa ggttctcaag gaatacgaac
     2461 ggggtgt
//
LOCUS       DATMKH010000649         1053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3808851, whole genome shotgun sequence.
ACCESSION   DATMKH010000649 DATMKH010000000
VERSION     DATMKH010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3808851"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..>1053
                     /gene="glmS"
                     /locus_tag="VNV63_07750"
     CDS             65..>1053
                     /gene="glmS"
                     /locus_tag="VNV63_07750"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF001484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HXC62553.1"
                     /translation="MCGIVGYIGSQKVVPIVLDSLKRLEYRGYDSAGVAFLENGHLEV
                     KRCIGKLSHLETSLAGQSYGGTIGLGHTRWATHGRPSEENAHPHRVGGIAIVHNGIIE
                     NYLPLKKRLIAAGVRFKSETDTEVMVQLLAQYKKKGSSLEEAVRQVLKEVRGSYAIGV
                     ISEDEPDMLIAARNGCPLVLGVGRDETFMASDIPAILHHTREIIFLDDGEMAVLTSKG
                     AEVKSLTGKRLNKTVSTITWSAGMAEKSGYPHFMLKEIHEQPRAILDTIRGRIDFDSG
                     DVHLEELKLTQRDFSRLQKIMLVACGTSWHAALVGKYLLEELTHLPVEVDISS"
BASE COUNT          261 a          255 c          310 g          227 t
ORIGIN      
        1 aagaaaaaaa cgaaaaagta ggggtgcaat gcattgcgcc ctgacaaaga aggggcttta
       61 aaatatgtgc ggcattgtcg gatatatcgg ttcacaaaaa gtggttccta ttgttctgga
      121 ctccctgaag cgtcttgagt atcggggtta tgactctgcc ggggtcgcgt ttttagaaaa
      181 tggtcatctg gaggtgaaac gctgtatcgg aaagctttca catcttgaaa ccagccttgc
      241 gggtcaatcc tatggaggga ccatcggcct gggtcataca cggtgggcga cgcatggaag
      301 gccgtcggag gaaaacgccc atccccatcg ggtagggggc atcgcgatcg ttcacaacgg
      361 gatcattgaa aactatctcc ctctcaaaaa acgtctgatc gccgccggcg ttcgttttaa
      421 atccgagacc gataccgagg tgatggttca gttgctcgcc cagtataaaa agaagggatc
      481 cagcctggag gaggcggtgc ggcaggtttt gaaagaggtc cgagggtctt acgcgatcgg
      541 cgtcatcagc gaagatgagc ccgatatgct gatcgccgca cgaaacggct gcccgctggt
      601 ccttggcgtc gggcgggatg agaccttcat ggcctcggac atccccgcga ttctccatca
      661 cacacgtgaa atcatttttc tggatgatgg agagatggcg gtgctgacga gcaaaggcgc
      721 ggaggttaag agtctgaccg gcaaacggct taacaaaact gtttctacaa ttacctggtc
      781 cgccgggatg gcggagaaga gcggctatcc ccatttcatg ctgaaggaga tccacgagca
      841 gccccgggca attctcgaca ccatccgagg gagaattgat tttgattccg gcgacgttca
      901 cctggaagaa ctgaaactca cgcaacggga tttcagcagg cttcaaaaga tcatgctggt
      961 cgcatgcgga acatcgtggc atgcggcgct cgtgggcaaa taccttttgg aagaactcac
     1021 ccatcttccg gtggaggtgg acattagctc gga
//
LOCUS       DATMKH010000650         1450 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1585732, whole genome shotgun sequence.
ACCESSION   DATMKH010000650 DATMKH010000000
VERSION     DATMKH010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1585732"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            782..1354
                     /locus_tag="VNV63_07755"
     CDS             782..1354
                     /locus_tag="VNV63_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62554.1"
                     /translation="MSPAAVVKLTDTSITDVKTLHRQPALIQELTWWLPMLPGASYTA
                     DSVREILFSFYNGELYKISATYDRAATEGLTAEDLAQSISAKYGPPIDSLTEIDLSTG
                     ERHGGTAETVVAHWGNAQYSLCLYRSGLANGFTLEMYSLQTNAAADLAIAKAIKVEEL
                     EGPQKEADRQKKEADELALARLKNLQSFRP"
BASE COUNT          349 a          351 c          408 g          342 t
ORIGIN      
        1 gcgcgccgcg ggttttcgcg gcttcggcgg aaccgttgtc acctccatgg tcgcggtgat
       61 cggagcagcc cttctaacca tggtgattgg attcgcaaac ggtcgaagga tttacgcccg
      121 agcatcctaa caggtcacga aacaggaacg aaagcttgtc gcggctatta agtttcagga
      181 aacgcctatg tcagtcaaaa acacgtccgg gaccgtcttc atcagagaga acacgctttt
      241 gccgacagga ttggcagtcg aaactgagct tttcttgcct ggctggagag tggttagaaa
      301 tctcgatggg tacgggcttg gccgaaaggt cgaggaggcg aactggaact ttttctatct
      361 ggctggtgat ctcaaggcag tcactcttgg tcggaaaggg ccaggaacgc tgcgcaaagt
      421 agtcagacgc ttgcttggaa aaccaggagg ggagaggttt aactccctgg agataacgaa
      481 agttgtttcg gaacgcttcc tgggtattcc ttttacgcgc gttacggccc attttcggca
      541 tatccaacaa gagattggcc ttcgggaccc cgcgaatgat tctgtctgga gagtatcatc
      601 ttcgccaaat ggagaagaac atttacaacc tggggctttg atttcaagtt cttaggacta
      661 tgtgggagaa atgaaaatga gaacgattcg cagttcaact ctttgtcttt ttgtcatgct
      721 acttgtggcg ccgctacttt gcgcacagga tctgtctaaa taccggggat tttcctttgg
      781 gatgagtccg gctgcagttg tgaagctgac ggacacgagt atcaccgatg tgaaaaccct
      841 tcatcggcaa ccggccttga ttcaggagct aacctggtgg ctgccgatgc ttcccggagc
      901 atcttataca gccgacagcg tgcgggagat tctcttctcc ttctacaacg gggagcttta
      961 caaaatatcc gcaacctatg accgggccgc cactgaaggg ctcacagcag aggatctggc
     1021 gcagtcgatc tccgctaagt acggcccacc gatagattcc ctgacagaaa ttgacttatc
     1081 gacaggtgaa cgacacggtg ggacggcaga gacggtggtc gctcactggg gaaatgccca
     1141 gtactctctc tgcctttacc gttctggttt ggcgaatggc ttcacccttg agatgtattc
     1201 cctgcagaca aatgccgcag ccgacctggc cattgccaag gccatcaaag tggaagagct
     1261 agaaggccca cagaaagagg ccgatcgcca gaaaaaagag gcggatgaac ttgcgctggc
     1321 tcggctgaag aatctgcaga gttttcgacc ttagtgtcga tctgtagtca ccaagggttg
     1381 agtgttcgag ttttggaagg aattgaacgg aggccaagga aatgactgtg agtattactc
     1441 ccctgcatga
//
LOCUS       DATMKH010000651         1570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1893287, whole genome shotgun sequence.
ACCESSION   DATMKH010000651 DATMKH010000000
VERSION     DATMKH010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1893287"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="VNV63_07760"
     CDS             <1..506
                     /locus_tag="VNV63_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HXC62555.1"
                     /translation="RWLTDFFGFAVKLVCEPTTGFPDDRLAAGPTIVSEGSLQEVARW
                     FPGLTFEGARRRFRTNLELDMVEPFWEDRLFGGSEELKPFRIGNVSFRGHNPCQRCVV
                     PSRDPESGIQSVPSFQKVFMAARKGSLPPWANRDRFNHYYRFAVNTSIPSSEAGKILR
                     VGDTVEC"
BASE COUNT          360 a          420 c          431 g          359 t
ORIGIN      
        1 atcggtggtt aactgacttt tttggattcg ccgttaagct tgtctgcgag cccaccaccg
       61 gctttcccga tgatcgctta gcggccggtc caacgattgt cagtgaaggg agcctgcagg
      121 aggtcgctcg ttggtttccg gggctgacat tcgaaggagc gcgtcgcaga ttccggacga
      181 atcttgaact cgacatggtg gagccgttct gggaggaccg ccttttcggc gggtcggagg
      241 aactcaaacc tttccggatc ggcaacgtct ccttccgagg ccacaaccca tgccaacgct
      301 gcgtggttcc ctctcgggat cccgagagcg ggattcaatc ggtgccgagc tttcaaaagg
      361 tcttcatggc cgctcggaaa ggatcccttc cgccgtgggc caaccgggat cgatttaacc
      421 attactaccg ttttgcggtg aacacttcga ttccatcctc ggaagcgggt aagattctcc
      481 gcgtcgggga caccgtcgaa tgctgaggtc cgccgcattc tggggcgttg atgctactcc
      541 ggattcattc ctttcgtcgc attttggttc tcgttccgga atggcaaatg aatacctgct
      601 gttcgaagac tggacagcac tgaaactgct tgaatgaccg atagagttct ctccttgacc
      661 ggttagcgaa ttctccgaat tgcgaataac ctcgacttac tggccgaccc ggacgagtat
      721 ctcccctatc tctctcttcc gctggtgcgc cagggcgcct ttagtttgga cggggcctcg
      781 catctccggc gaaggcagac gtagaaagtg gcgtcaatcg ccccagtaaa aggatcacaa
      841 attgccaaaa ccaaattatc agttcgaaaa gcggaaaaga gacatggaaa agaaggccaa
      901 gaaggaagaa aagaagctgc gaaagcttcg tccctccgat ccgtctccct ctgttccccc
      961 tacagacgcg aagtaagtcg caagcagaag cggttctgtc tattctggtg cgttgccaag
     1021 caggtttttt ggcttactcg accaggcggc ccgtcatgat gtgtggaccc ggtcagtcgg
     1081 cctcaatcac gacaatcgct agtcgaagcc atcttatccg caagtctgtc ggtcaaaacc
     1141 tggctcgcta tttctgaccc cgctggaaaa aacgagctca ttttggctca aggaatccgg
     1201 tggaaaattg ttagttagaa gaccacgaga tgagttgttt gaactttatc gcttccatct
     1261 tggtctccac cacattttag ctttgttggg aaaggcgcct tgcgttagca cgaaaaacgg
     1321 tccggtcgtg gagaggaaca gaggactagt actagtgata aacacaagac tgcaaaagat
     1381 gcagcaaggg ctggtaagcc cgatctggaa gcggataggt aagtctgcga caaaggcaag
     1441 gctggatcaa tgagcctgaa aaacggcacg gcctatgtgc cgcgccggaa gcgggagccg
     1501 ccgtcttccc gatgccgatc ctgacgggtt ttcgccgatt ctgagcatga gcggtcctca
     1561 aacgaccaac
//
LOCUS       DATMKH010000652         1015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4132990, whole genome shotgun sequence.
ACCESSION   DATMKH010000652 DATMKH010000000
VERSION     DATMKH010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4132990"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(440..>1015)
                     /locus_tag="VNV63_07765"
     CDS             complement(440..>1015)
                     /locus_tag="VNV63_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62556.1"
                     /translation="LKSATEKGKAIKHLDGTPLTPESIDRYITDHSVANQPSILLKLD
                     LQGVDAVRFFCKVALATGFYVADESFGRSPVADKLRTVIRSQNIAEELMPGVFWPFHK
                     NTEAFRYFCVPDTHILAILPDPPVLAISLFGGTYTALVPLHEAGSFEWPLKRDGRVFQ
                     FSLKDKQFKQRSYDDYLSARPWLPPSTPQSS"
BASE COUNT          265 a          246 c          298 g          206 t
ORIGIN      
        1 ttcaccgact ataacgacat cgtgcccgac catcgggatc caaaagggat gggcggagcg
       61 tggagagatg accatcctga caacatccaa gcaacccatt ggtggtgtaa cgggaacaaa
      121 gggtcaataa ggatggctga ctgacggccc atgattcgga cgctgtcccg tctggccgtc
      181 aaatccggtc attcgagttg atggtgcgct agttgtgtgc cggatttcag gttcaggcat
      241 tcgcaacgca gaactcaggc ccatccggat catgaatgat aaacgcttcc cgttgcagaa
      301 accggagcga acgcaagcct ccttcgatgg gacccgtctg cacgagcgca ctacagcgaa
      361 gaagatggga gaaggtgctt gcgttcaagg gtggaggctt tctgctaagg ggttaaagcg
      421 gcaacaacga agcggagact taagaggatt gcggagttga tgggggaagc cacggccggg
      481 ctgacaggta gtcatcgtaa gaccgttgct tgaactgctt gtctttcagg gagaactgga
      541 aaacgcggcc atccctcttg agcggccatt caaacgatcc cgcttcgtgg agaggcacaa
      601 gggcggtata cgttcctcca aacagggaga tcgccaagac gggaggatca ggcaaaatag
      661 ccagaatgtg cgtatcggga acacagaaat accggaaggc ttcagtattt ttatgaaaag
      721 gccagaaaac tcccggcatc aactcttctg caatgttctg tgaccgtatt acggttcgga
      781 gcttatcggc aaccggagag cgtccgaagg attcgtcggc gacgtagaaa ccggtcgcaa
      841 gagccacttt gcaaaagaaa cgaacagcat cgaccccttg caaatcaagc ttcaagagga
      901 tgctgggctg attagcaaca gaatggtccg taatgtagcg atcaatggat tcaggcgtca
      961 gcggtgtgcc gtccagatgc tttatcgctt tgcctttctc ggtggccgac ttaag
//
LOCUS       DATMKH010000653         1253 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4472196, whole genome shotgun sequence.
ACCESSION   DATMKH010000653 DATMKH010000000
VERSION     DATMKH010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4472196"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..995)
                     /gene="dnaN"
                     /locus_tag="VNV63_07770"
     CDS             complement(<1..995)
                     /gene="dnaN"
                     /locus_tag="VNV63_07770"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HXC62557.1"
                     /translation="MEFTVNKADLVRELNLSQGVVEKKTTIPILSNILLEAKGGRVFL
                     TATDLELGIRCSCPAKVKKEGAGTVPAKRLLDYVRLLPDTDLTIKFLENHWASLICGR
                     SRTKMAGMSRESFPELPQVPEATNRISTTMLSSMIAKTIFAISMEESRFTLNGALLVI
                     RPEGVVMVSTDGHRLAYIENVAENGAENKPFQALVPKKAMSEILKLSQEAEADAKTVI
                     SGDDNHLFFQFGERLLITRKLTGNFPDYERVLPKDHHQVVTLQRDEIKSAVERVAQFA
                     DERSRAIRVQFGPGEVKVFTSSVETGESEESVPATYAGPEIDIGFNAQYLLDFLRA"
BASE COUNT          289 a          365 c          293 g          306 t
ORIGIN      
        1 gcccgcagga aatccaaaag atattgcgca ttgaatccaa tatcgatctc agggccggcg
       61 taagttgccg gaacgctctc ttcgctctct ccagtctcca ccgacgatgt gaaaactttc
      121 acttctcccg gcccaaactg cacccggatc gcgcgcgatc tctcatcggc aaactgtgca
      181 acccgctcca ctgccgattt gatttcgtct cgctgcaacg tcactacctg gtgatgatct
      241 ttcggcaata cacgctcata atcgggaaag ttcccagtca gcttgcgcgt aatcagcaga
      301 cgctcgccaa attggaagaa gagatggtta tcgtctcccg atatgacggt tttggcgtca
      361 gcctcggctt cttgcgacag cttcaaaatc tcactcatcg ccttcttcgg cacaagcgcc
      421 tggaatggct tgttctcagc cccgttctcc gcaacgtttt cgatataagc gaggcgatga
      481 ccatctgtcg aaaccatcac cactccctcg ggcctaatta ccagtaaggc tccgttcaga
      541 gtgaatctgg attcttccat cgagattgca aaaatagtct tcgcaatcat ggaagacaac
      601 atcgtagtcg agatccgatt ggtcgcctcc ggcacttgcg gaagctccgg gaagctctcc
      661 cgcgacatgc ccgccatctt ggtgcgcgac cggccgcaga ttagactcgc ccagtggttt
      721 tccaaaaact taatcgtcag gtccgtatcc ggaagcaggc ggacataatc gagcagcctc
      781 tttgccggta cggttccagc gccttccttc ttcactttgg caggacacga acagcgaatg
      841 cccaactcca ggtcggtcgc cgtcaggaac acacggccac ccttagcttc cagcaggata
      901 ttggagagaa tcggaatggt cgtcttcttt tccaccaccc cctgagagag attcaactcg
      961 cggaccaggt ccgccttgtt aacggtgaat tccatcgact gcctgctcct atacttcctg
     1021 ctctgttaat ctaaggtgct tatttagaac cgtattcata ggtcctgtga aaaagttgga
     1081 atctatctaa attatacaaa aatttatagg ttcctgtctc gctcagttga ggaaaaaagg
     1141 tagcggcccg acgaaaaaac aggcctccca agtctttctc ctcaggcccg cgcccaggtt
     1201 tgggccgggg gctgtgggaa cctttgccat ggctattgta atgagtcaat tag
//
LOCUS       DATMKH010000654         2192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4767536, whole genome shotgun sequence.
ACCESSION   DATMKH010000654 DATMKH010000000
VERSION     DATMKH010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4767536"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..1071)
                     /locus_tag="VNV63_07775"
     CDS             complement(55..1071)
                     /locus_tag="VNV63_07775"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HXC62558.1"
                     /translation="MRPIEEIVQGAGAVDASDILLSQNTQLYFRVDGEVQPQGPCPDF
                     ISELNRLVPRLEEALKHKSGAADLPVVIAGQRFRATVYKERTGLSAALRFIPEKVVPA
                     AELGIDSFLLDKVKASRQGLILVTGPSGCGKSTTVNCLVDHLNQTRRDHILTIEDPIE
                     FVYTPARSAIDQREMGEHFDTFSAAIRSAVRSNPDVIVVGEVRDYETARAVLQAAETG
                     LLVFATLHTKRVYNTISRLIGMSPAEEKEDIRSTLANNLLMIISQLLLKRVGGGRVAA
                     REIMMQTDSTANLIRESKEKTIEGVLATHREQGMINLTSSLKTLYGNSLIDEQTFAEN
                     FSAA"
     gene            complement(1085..>2192)
                     /locus_tag="VNV63_07780"
     CDS             complement(1085..>2192)
                     /locus_tag="VNV63_07780"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HXC62559.1"
                     /translation="AVLKVERKREKEEEKINRVRIPLKVKINFFQQMATCYKLQIHLA
                     KAFDIMQEACANKTFKAILHEVKEEIESGGKINASIARYPRIFDPVTLALVAVGEEAG
                     VMEKVCRQIKILLARNQQLQKKLRSIMIYPVTVCSVLTVSLYILTTQVIPKFAVIFES
                     VHMSLPLPTQILMSISGVASAHPLLTLGLAGAFLYFVAQFPRIFRQVHQLHRLSLRLP
                     IIGVIQRRVIMANFARTFSNLIDANVALIRSLSLVRDVSPNFCYRAMIAQAILAVNEG
                     RKMSPQMTDTDVFNKDFVRLLQFADETAQTSAILAPLADELDNELNVLIDDLKPVIES
                     LIIVVIGIILGGVLLALFLPIFNISEAIRAEAGR"
BASE COUNT          472 a          570 c          617 g          533 t
ORIGIN      
        1 gaaaaatgtc ttgagatagt agcgccatgg ataattggac agaaactgta acatttacgc
       61 tgcgctgaag ttttccgcaa aagtttgttc gtcgattaaa ctgttgccgt acagggtttt
      121 aagggagctt gtcaagttga tcattccctg ttcccggtga gtcgctaaca ccccttcgat
      181 tgttttttcc ttgctctcgc ggatcaagtt tgccgtggaa tcggtctgca tcatgatctc
      241 tcgggctgcc actctgcctc ctcccacccg tttcaggagg agttggctga tgatcatcag
      301 gaggttgttg gccagggtag agcggatatc ttccttttct tccgccggtg acattccaat
      361 caaacggctg atcgtattat agacccgctt cgtatggagc gtggcaaaaa ccagaagccc
      421 ggtctcggca gcctgaagga cggccctcgc agtttcgtaa tctctaacct caccgaccac
      481 gatcacatcg gggtttgagc gtaccgcgct gcggattgca gccgagaatg tgtcaaagtg
      541 ctcccccatc tcgcgctgat cgatcgcgct cctggccggc gtataaacga actcgatcgg
      601 gtcctcaatc gtcaggatgt ggtctcttct agtctgattg agatggtcga ccaggcaatt
      661 gaccgtggtg gatttaccgc agccggatgg cccggtcacc aggatgagtc cctggcggga
      721 ggccttaact ttgtccagga ggaatgaatc aatgcctagt tcagcggccg gaacgacttt
      781 ctccgggatg aaccgaagcg cggccgagag gccggttcgt tccttataga ccgtggctct
      841 gaaccgctgg ccggcaatga ccaccggaag gtcggcggct ccgcttttgt gtttgagcgc
      901 ttcctcgagt ctcgggacca gccgattcaa ttcggagatg aagtctggac aaggcccttg
      961 aggctgcact tccccatcca cgcgaaagta aagctgcgta ttctggctca agaggatatc
     1021 ggaggcgtcc actgcaccgg ccccctggac tatctcttcg atcggtctca tggcgtcaga
     1081 atagttacct gccggcttcg gccctgatcg cttcggagat gttaaagatc ggtaagaaca
     1141 gggctaatag aacgcctccg agaatgatcc cgatgacgac aataatgagc gactcgatca
     1201 cgggcttcag gtcatcaatc aagacattga gctcgttatc cagctcgtcg gcgagcgggg
     1261 ccaggatggc ggaggtctgg gcggtttcat ccgcgaattg gaggagcctg acaaagtcct
     1321 tgttgaagac atcggtgtcg gtcatctgcg gcgacatctt ccgtccctcg ttcacggcca
     1381 gaatggcctg ggcgatcatc gcgcgatagc agaaattcgg ggagacgtcc ctgaccaggc
     1441 tcagagagcg aatgagtgcg acattggcat cgatcaggtt actgaacgtt cgggcgaaat
     1501 tcgccataat cacccggcgc tgaatgacgc cgatgatggg gagtcgtaac gaaagccggt
     1561 gaagttgatg aacttgcctg aaaatccggg gaaactgagc aacgaaataa aggaatgctc
     1621 ccgccagtcc cagcgttagc agcgggtgtg cgctggcaac cccggagata ctcatcagga
     1681 tctgcgtcgg caagggaagg ctcatgtgga cggattcaaa tatcaccgca aatttcggga
     1741 tcacctgggt cgtcaggata tacagcgaaa cagtgaggac cgaacaaacg gttaccgggt
     1801 agatcatgat ggagcggagt tttttctgca gctgctggtt gcgcgccagc aggatcttga
     1861 tttggcggca cactttttcc atcacgccgg cttcttctcc caccgcgacc agggcaagcg
     1921 tgacgggatc aaatattctc ggatacctgg cgattgaggc gttgattttt ccgccgcttt
     1981 caatttcctc tttgacctcg tgaaggatgg ccttgaacgt tttgttcgcg caggcctctt
     2041 gcatgatgtc aaaggctttt gccagatgga tctgcagctt gtagcaggtg gccatctgtt
     2101 gaaagaagtt gatctttacc ttaagaggga ttctgacccg gttgatcttt tcttcttctt
     2161 tctccctttt ccgctcgact ttgagaacgg ct
//
LOCUS       DATMKH010000655         1229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3495476, whole genome shotgun sequence.
ACCESSION   DATMKH010000655 DATMKH010000000
VERSION     DATMKH010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1229
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3495476"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="VNV63_07785"
     CDS             <1..510
                     /locus_tag="VNV63_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62560.1"
                     /translation="AMYTHGIKLNESKKAPKVTQIYDANGKIRHAVVYTDEDGNEQQL
                     MLDQGITPENLNKGKGGGKKGGSGSDDSLKKMKEFNRAYEKARSEISDPSKAGQMKAT
                     DSKGFEEKMQWLKDNQDQYDANIKALGKGGGAEIPGQAPGTGTPAAPDNAPFDADAFI
                     KDALGQGNK"
     gene            510..>1229
                     /locus_tag="VNV63_07790"
     CDS             510..>1229
                     /locus_tag="VNV63_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62561.1"
                     /translation="MAKLDEYWDQIQNSDSFQNLDTAQKEKVRNSLWDKYVQKSPSYT
                     NLPLSQRNQVRSHFDELTHMKQTSMMDDLEKKGKKIVGEVKPMAEKVGQAVSGAVDVS
                     KVGSDIEDMASTSVEIPGMKKVAGAVSGAAEKAGSAIEPNFVKPQLTNFDVARAIGAG
                     AVRTMGDVLAGIVPTTTMQAAAYFSDPVLKYAGTTFLGRDIHLPGWKPDTGMETADVS
                     VGSQFEKIITQRRIENNARQAT"
BASE COUNT          348 a          304 c          365 g          212 t
ORIGIN      
        1 gcgatgtaca cgcacggaat caaactaaac gaatcaaaga aagcgccgaa ggttacgcag
       61 atctatgacg cgaatgggaa gatccgacac gcggttgtct acacggatga ggatggaaat
      121 gagcagcagc tcatgctaga tcagggtatc acgccggaga atttgaataa gggtaagggc
      181 ggaggcaaga agggcggatc gggttcggac gactctctga agaaaatgaa ggaatttaat
      241 cgtgcctatg agaaggctcg ctcagagatt tccgatccct cgaaggcggg gcagatgaag
      301 gcgacagact ccaaaggctt tgaagaaaag atgcaatggc ttaaagataa ccaggaccag
      361 tatgatgcca atatcaaagc cctcggtaag ggtggaggcg cggagatacc gggacaagcg
      421 ccgggaactg ggactccggc ggcacccgat aacgccccct ttgacgcgga tgctttcatc
      481 aaagacgccc tagggcaagg gaataaataa tggcgaagct ggacgaatac tgggaccaga
      541 tccaaaattc tgattccttc cagaacctgg acaccgcaca gaaggaaaag gtgcggaaca
      601 gcctctggga taaatacgtc cagaagagtc cgtcctatac caaccttccg ctgtctcagc
      661 gcaaccaagt ccgcagccat ttcgatgaac tgacccatat gaaacagacc tcgatgatgg
      721 acgacttgga gaagaaggga aaaaagatcg tcggagaagt taagccgatg gcggagaagg
      781 tcggtcaagc cgtatccgga gcggtcgacg tgtccaaagt cggctctgac atcgaggaca
      841 tggcctcgac ctccgtagag attccgggta tgaagaaggt ggccggtgcg gtctcgggcg
      901 ccgcggagaa ggcggggtcc gccattgaac cgaacttcgt caagccccag ctcactaatt
      961 ttgatgtcgc gcgcgctata ggcgccggtg ccgtgagaac aatgggagac gtcttagcgg
     1021 gaatcgttcc aacgacgacg atgcaggcgg ccgcctactt ctccgatccc gtcctcaaat
     1081 acgcgggcac aacattctta ggccgtgaca tccacctccc gggatggaaa cccgatacag
     1141 gcatggagac ggcggacgta tccgttggtt ctcagtttga aaagatcatc acgcaaagac
     1201 gcattgaaaa caatgcgagg caggcgacg
//
LOCUS       DATMKH010000656         1406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4134087, whole genome shotgun sequence.
ACCESSION   DATMKH010000656 DATMKH010000000
VERSION     DATMKH010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1406
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4134087"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1005)
                     /locus_tag="VNV63_07795"
     CDS             complement(<1..1005)
                     /locus_tag="VNV63_07795"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HXC62562.1"
                     /translation="MKNNHLLLLLAIAVVVGLAGIYFQISQSAGWSDAKTDRTILQNL
                     PVNDIKKILIRSSPATVTLEKKGDQWSVTERNDYPADFTKIRDLIKMLWELKAGQETQ
                     VGPSQLGRLKLLTPGQGAESGIEIDLKGEKENQIATLIIGKSVDRSNAAAGSAESGRF
                     IYNAAVKDRVYLVSESFLSIDPVNVAPWLDKTFIAPGEAKEIDQAAWSNNPGWKVIRK
                     DPKAEWQLEDLQTGETLDKSFAQTLSNFAPTFTDVRPLSVSPDETGLKDPFRITVKTF
                     DGFIYDLLVGKEGPDKTRFLQLSVSAELPSARTPAPDESTEDKKKKDEEFDKKNTGLK
                     E"
     gene            complement(1002..>1406)
                     /locus_tag="VNV63_07800"
     CDS             complement(1002..>1406)
                     /locus_tag="VNV63_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62563.1"
                     /translation="DSDLIGIRSRSSGNRPFIVVNKMEAAALEKYQSKIDDLEDNLNQ
                     TRQKLAALQTSKQSDQKTLLSPEQQAEIKKFQENEATVNKELKQLRKNLRQEIDSLQS
                     TVKWVDIAGMAVIVTLVGLTLAVVKRRRRAAR"
BASE COUNT          280 a          374 c          361 g          391 t
ORIGIN      
        1 ctctttcaga ccggtgttct ttttatcgaa ctcttcgtct ttctttttct tgtcctcggt
       61 actttcatca ggggccggtg ttcgcgccga aggtagctcg gcgctgacgc ttagctgcaa
      121 aaagcgtgtt ttatccggtc cttccttgcc gacaagtaag tcgtagatga aaccgtcgaa
      181 cgttttaacg gtgattctga acggatcctt taacccggtc tcgtcagggg aaacggagag
      241 cggacgaacg tctgtgaagg tgggggcaaa gttggagaga gtttgcgcga atgacttatc
      301 caaggtttcg ccagtctgca gatcctctaa ttgccattcc gcttttggat ccttgcgaat
      361 gactttccag cccgggttat tggaccaggc cgcctgatcg atctcctttg cttctcccgg
      421 ggcgatgaac gttttgtcca gccagggcgc cacattgacc gggtcgatgc tcaaaaagga
      481 ttcggaaacc aggtagacac gatccttgac ggccgcgtta tagataaatc ttccgctctc
      541 tgctgagccg gccgccgcat tgctacggtc cacggatttg ccaatgatca gtgtagcgat
      601 ctggttctct ttctcgcctt tcaggtcgat ttcaatgccc gactccgcgc cctggcccgg
      661 cgtgaggagc ttgagccggc caagctgcga tggtccaacc tgcgtttctt gcccggcttt
      721 caattcccac aacatcttga tcaaatcccg gatcttggtg aaatcggcag gatagtcatt
      781 tctttcggtc accgaccatt ggtcaccctt cttttcgaga gtcaccgtgg cgggtgaaga
      841 gcgaatgagg attttcttga tatcgttgac cggcaggttt tgcaaaatcg ttcgatccgt
      901 cttggcatca ctccatccgg cagattgtga gatctggaaa tagatcccgg ccaaaccaac
      961 gaccaccgca atcgcaagca gtaacaggag atggttattc ttcatcgcgc agcgcgtcta
     1021 cgacgtttca cgaccgccag ggtcaaaccg acgagggtga cgatcacggc cattccggct
     1081 atatccaccc atttgaccgt actctggagg gagtctatct cctgacgaag attcttgcgg
     1141 agctgcttta gttccttatt caccgtggcc tcgttctcct gaaatttttt gatctcagcc
     1201 tgttgctctg gagacaagag cgttttctga tcagactgtt tgctggtttg cagggccgcc
     1261 aatttctgcc gtgtctgatt taaattgtct tccaagtcgt cgattttact ctggtatttt
     1321 tcgagggcgg cagcctccat cttgttgacg acaataaatg gtcgattgcc ggatgatctg
     1381 ctccggatac caatcaggtc ggagtc
//
LOCUS       DATMKH010000657         1431 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3496120, whole genome shotgun sequence.
ACCESSION   DATMKH010000657 DATMKH010000000
VERSION     DATMKH010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3496120"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1366
                     /locus_tag="VNV63_07805"
     CDS             <1..1366
                     /locus_tag="VNV63_07805"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HXC62564.1"
                     /translation="GSNSFLLESVGGTSRYSFIGVNPDFVFRSKGTSIEIEGAFGKTA
                     FQANPVEMLKKLLFSRMATRHHRLPPFFGGAVGLFGFDLVKFYERLPQHAMDDLDLPD
                     LCFLFVNSVIAFDHEQKTIQILYTPTAEEFFGLERENLYRHVVTKIKDLQERLLNETS
                     MLQISSMHAVALSDRPRSLTTREEYLGMVQRCKDYIAAGDIFQANLSHRFSIPIGQTP
                     PLCLYKALRRINPSPFSAYLDFGSFQIVSSSPERLVKLEGREVQTRPIAGTRPRGRSP
                     LEDRQFAEELILNEKERAEHLMLVDLERNDLGRVCEYGTVRVDELMTIEFYSHVMHIV
                     SNIRGRMRPDRDCFDLLKAVFPGGTITGVPKVRCLEIIEELEPVKRGAAYGSIGYINF
                     AGDMDLNILIRTMIVKNKEAYFHVGAGIVADSQLEKEYEETLHKAQALFLALQEAKAD
                     GHLY"
BASE COUNT          321 a          364 c          374 g          372 t
ORIGIN      
        1 ggggtcaaac tcttttttgc ttgagagcgt tggcggcact tcacgctact ctttcattgg
       61 cgtgaatccc gacttcgtat tccggagtaa agggacctct atcgagatcg agggtgcatt
      121 cggcaaaacc gcttttcaag caaatccggt cgagatgctc aagaaacttt tgttttcaag
      181 aatggcaacc cgtcatcatc ggcttccacc tttttttggc ggcgccgtgg gattgtttgg
      241 ctttgacctt gtcaaattct acgaacgcct tccgcagcat gcgatggacg atctcgacct
      301 ccccgatctt tgctttctct ttgtaaattc ggttatcgcc tttgatcacg agcagaagac
      361 cattcagatt ctctacacgc ccaccgcaga ggagtttttt ggactcgagc gggaaaattt
      421 gtaccggcat gtggtcacaa agatcaagga tctgcaggag cggttgctaa acgaaacatc
      481 gatgctccag atttcgtcca tgcatgccgt ggccctttcg gatcgtcctc gttctctcac
      541 gacccgggag gagtatctcg ggatggtcca gcgctgtaaa gactatattg cggcgggaga
      601 catctttcag gcaaatctct cacaccgttt ttcaatccct attggtcaga caccgcccct
      661 ttgtctctat aaagcgcttc gacgaatcaa tccttccccc ttctcggcct atctagactt
      721 tggctcgttc cagatcgtaa gctcatctcc cgaacggctc gtcaagctcg agggtagaga
      781 ggtccagacg agaccgatcg cgggcacacg accgcgcggc cgctctccct tggaagaccg
      841 gcagtttgca gaggagttga tcctcaatga gaaggaacgg gcagagcatc tcatgctggt
      901 tgacctcgag agaaatgacc tcggccgtgt ctgcgaatat ggtaccgttc gggtggatga
      961 gctgatgaca atcgagtttt actcccatgt gatgcacatt gtctccaaca ttcggggtcg
     1021 aatgcgacct gatcgggact gttttgatct gctcaaggcg gtgtttccgg gtggaacaat
     1081 tacgggtgtg ccgaaggttc gatgcctaga aatcattgag gaactggaac cggtaaaacg
     1141 cggcgcggcg tatggatcta tcggatacat aaactttgca ggggacatgg atctgaacat
     1201 cctgattcgg acaatgatcg taaaaaacaa ggaggcctat ttccatgtcg gtgcaggtat
     1261 tgtggccgac tcccagcttg aaaaagaata cgaggagacc cttcacaagg ctcaagcgct
     1321 ctttttggcg ttgcaagagg caaaggctga tggtcattta tattaatggc gcttatcttc
     1381 cccgagaccg ggccaacgtc tctgtctttg accggggttt tcttcttgga g
//
LOCUS       DATMKH010000658         2633 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2535364, whole genome shotgun sequence.
ACCESSION   DATMKH010000658 DATMKH010000000
VERSION     DATMKH010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2535364"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..932)
                     /gene="mutL"
                     /locus_tag="VNV63_07810"
     CDS             complement(<1..932)
                     /gene="mutL"
                     /locus_tag="VNV63_07810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HXC62565.1"
                     /translation="MASRIQILPETLINLIAAGEVVERPASVAKELVENAMDAGSRCI
                     TIEVEDGGKRLIRVTDDGEGMSREDALLAFQRHATSKIKTDADLEAIATMGFRGEALP
                     SIGSVSKIRLITRQAGDEVATKLVLEGGNMKEVAEAASPPGTMIEVAQLFYNTPARRK
                     FLKAAGTELAHISGLVTELALAHPHLEFRFLQDGKSVFHFPGAEDWHQRTIQIFGAEM
                     AEQLIPVETHQGRIRITGGVSRPHYTRGAKTHQFFFVNGRSIRDRLLAHALYEAYDTL
                     LMKGRHPVAILFIELDSSMVDVNVHPSKREVRFRT"
     gene            complement(937..1809)
                     /gene="ybgF"
                     /locus_tag="VNV63_07815"
     CDS             complement(937..1809)
                     /gene="ybgF"
                     /locus_tag="VNV63_07815"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HXC62566.1"
                     /translation="MRTKSSIPILLVLGFLSVSMGCALHADLVDTQLDLDNIKQQQAQ
                     IQKRLETIENYFKERAGSVQRGQADIVIKVDQLGVDFQVLEGKLEELNRLIAQLSVRL
                     DDQTFRIAQSGSRLDALETRASGAPQAINPPRGQAPEGGEPTSETTSPDRKTMIPGRT
                     PGQSVSILPTEAYNLAYNDYIKGNYDLALIGFQNYISQFPDTSLSPDAQYWIGQVYYT
                     RKDYRKAIEAFALVVQKYSKSDAVARSLLQEGLAYLELGDKTQARAAFRRVVQDYPFS
                     EESKLAKNRLAEMR"
     gene            complement(1836..2405)
                     /gene="pal"
                     /locus_tag="VNV63_07820"
     CDS             complement(1836..2405)
                     /gene="pal"
                     /locus_tag="VNV63_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HXC62567.1"
                     /translation="MRQIIDRRQTWFLLILMGMMFSLVAAGCAKKVETASQTVSPEEN
                     VTSPPPPAEEPQKEERPAVKEAEVAPPETSKPISSVSLEDIHFDYDKSAIRPDVKAIL
                     EKHAKWLQSNPKVKIQIEGHCDERGTNEYNLALGERRAQATKRFLVAMGIDAKRLSTI
                     SYGEERPLCSEHAESCYSKNRRAHFVIEK"
BASE COUNT          573 a          774 c          658 g          628 t
ORIGIN      
        1 gttcgaaaac ggacttcacg tttggaggga tggacattta catccaccat cgaagagtct
       61 aattcgataa agaggatcgc gaccggatga cgtcccttca tcaacagtgt gtcgtaggcc
      121 tcgtacaggg catgggccaa cagcctgtcg cgaatactcc gtccattcac gaaaaagaac
      181 tgatgcgtct tcgcgccgcg ggtataatga ggacgggaca caccgccggt gatgcgtatc
      241 cgcccttgat gggtctcgac cggaatcagt tgctcggcca tctcggcccc aaagatctga
      301 atcgttcttt gatgccagtc ttctgcgccg ggaaagtgaa agacgctctt cccatcctga
      361 aggaaacgaa attcaagatg cggatgggcc aaggcgagtt cggtcacgag accgctgatg
      421 tgggccagct ccgtgccggc tgctttaaga aatttccggc gggccggtgt gttgtagaac
      481 agttgagcaa cctcgatcat cgtgccggga ggcgatgctg cttcagccac ctccttcatg
      541 ttcccgcctt ctaagacgag cttggtggcg acttcatcac cggcttgccg ggtaatcaga
      601 cggatcttgg agacagaacc gatactgggg agcgcctcgc cgcggaatcc catggtggcg
      661 atggcttcaa gatccgcatc ggttttgatt ttgcttgtgg catgacgctg aaaggccaag
      721 agggcatcct cccggctcat cccctccccg tcgtccgtga cccggatcaa tctcttcccg
      781 ccgtcctcca cctcaatcgt tatgcaacgg cttcccgcat ccatcgcatt ctcgactaat
      841 tccttcgcca cggaagcagg ccgttccacc acttcacctg ccgcgatcag gttgatgagg
      901 gtctccggga ggatctgaat cctggatgcc atcaagttac ctcatctcag ccagccgatt
      961 cttagccagc tttgactcct ccgaaaacgg ataatcctga accacgcggc ggaaagccgc
     1021 cctggcctgc gtcttgtcac ccagctctag ataggccaga ccctcctgaa gaagagacct
     1081 ggccaccgcg tcgctcttcg aatatttctg cacgacgagg gcaaaagctt caatcgcttt
     1141 acgataatcc tttcgggtgt agtagacctg accgatccag tactgagcat ccggcgagag
     1201 gctcgtatcc ggaaattgcg agatataatt ctgaaacccg atcaaggcca gatcataatt
     1261 gcccttgata tagtcattgt aggcgaggtt gtaggcttcg gtcggtaaaa tacttacgga
     1321 ctggcccggg gttcttcccg ggatcatggt tttccgatcg ggcgaggtcg tctccgaagt
     1381 cggctctcct ccttccggcg cttgaccgcg agggggatta atcgcctgag gcgccccgga
     1441 ggcgcgcgtc tctaaggcgt ccaaacggct ccccgactgt gcgattcgga aggtctgatc
     1501 atcaagccgc acggacaact gtgctatcaa acgattgagc tcctccaact tcccctccag
     1561 gacttgaaaa tctaccccga gctggtccac cttgatgaca atatccgctt gaccacgctg
     1621 aacggagccg gcccgttcct taaaataatt ctcgatcgtc tccagacgct tctggatctg
     1681 cgcctgctgt tgcttaatgt tgtccagatc gagctgggtg tccacgaggt ccgcatgcag
     1741 ggcacaaccc atgctcacag acaagaaacc caggacaagt aaaattggaa tcgagctttt
     1801 cgttcgcatc gaaccttctc ctctatgcga tttttttact tttcaatcac aaaatgggca
     1861 cgccgattct tcgagtagca gctctccgca tgctcgctgc aaagcggtcg ttcttcacca
     1921 taactgatcg tggacagtcg cttggcgtcg attcccatcg caaccagaaa gcgcttcgtc
     1981 gcctgagccc gccgctctcc caaagccaga ttgtattcat tcgttccacg ttcatcacag
     2041 tgaccttcaa tctgaatctt gacctttgga ttggactgca accacttggc gtgtttctcc
     2101 agtatggctt tgacgtcggg ccgaatggcg gatttatcat aatcaaaatg gatatcctcc
     2161 agagaaaccg aagaaattgg ttttgaagtc tccggggggg ccacctctgc ttccttgacc
     2221 gccggcctct cctccttctg tggctcctcg gcgggaggag gcggggacgt cacgttttct
     2281 tccggagaaa cagtctgcga cgccgtctcc accttctttg cgcaaccggc tgctacaaga
     2341 gagaacatca tacccattaa gatcaataga aaccatgtct gtcgccgatc aatgatttgt
     2401 ctcatctcta cttcctcctt tatatagaaa gatattctgt taaaagaaat tccgccgccg
     2461 gttcatactt aatttggtga ccaggccggt ccggtcagat tcaccgcctc cttcgtcaat
     2521 cgctccagcc cggtaccatc caggttgatt aaataaagct ggctgttccc gtcccgtatc
     2581 gaactaaaca caagatgccg cccatccggc gcccagctgg gtgattcggc atc
//
LOCUS       DATMKH010000659         1021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_625624,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000659 DATMKH010000000
VERSION     DATMKH010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625624"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="VNV63_07825"
     CDS             <1..386
                     /locus_tag="VNV63_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020146630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HXC62568.1"
                     /translation="IIWFPETGNPPSPMRIIRPKGDRIRHRRKYAEGNMRYHSFYFRG
                     PGNRHNLKAQNLAIFSQIAQGIDEETWLFHLHRQDYSKWFREAVKDPYLADQAARIEL
                     QFPQNSKESRKLIRALIDARYTLPE"
     gene            481..624
                     /locus_tag="VNV63_07830"
                     /pseudo
     CDS             481..624
                     /locus_tag="VNV63_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611550.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            824..>1021
                     /locus_tag="VNV63_07835"
     CDS             824..>1021
                     /locus_tag="VNV63_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62569.1"
                     /translation="MVNKALEDAAQEMKRDGKDDLRVMSLDDAPAFYADGKRSILDIH
                     DAIAAEYLPMPIEVFTAYFRVL"
BASE COUNT          248 a          247 c          277 g          249 t
ORIGIN      
        1 ctattatctg gtttcctgag actggaaacc ctccatcccc gatgaggatc attcggccca
       61 aaggagatcg tatccgtcat cgtcgaaaat acgctgaagg caatatgcgc tatcacagct
      121 tttattttcg cggaccgggg aaccgtcaca atctcaaggc ccagaatctt gctattttct
      181 cgcagattgc tcaaggaatc gacgaggaaa cctggctttt ccatctgcac cgtcaagact
      241 attccaagtg gtttcgtgaa gcggtgaagg atccttacct ggcagaccaa gcagctcgga
      301 tagaacttca atttcctcaa aattccaagg aatctcgaaa acttatacgt gccctaatcg
      361 atgcgagata tacgcttcca gaataatccc gcggcaatta accggcctcc gtttttctga
      421 accagcttaa aagttagtgg tcggcgagac cttgaccagc tgagggaggg ggttttgctg
      481 atgaagaaga agcgattcag tgtggggcag atcgtgaggg cgctgaagca agcagaggtt
      541 ggcgtccccg tcgtggtggt gatccgcgag gccgggatcg gcaagcaggc cttctaccag
      601 tgggaggcaa agtatattgg ccttgaggtg gctaggttga gcaacacgat accttcagtc
      661 aatctaacca tcaacgtcgg ctcttccggc ctcaagtttt tcctttatcg aatgcgtcac
      721 tccgcgactg aagttgaccc tgatggtgtc agggaagctg gagcgaattg gctggggttt
      781 agcggttaca catgcagctt ccagtgatac gtcggagatt tctatggtga acaaggcatt
      841 ggaggacgcg gcgcaggaga tgaagcgcga tggtaaagac gatcttcgcg ttatgagcct
      901 tgatgacgcc ccggcatttt atgcagatgg gaagcgatcc atattggaca tacatgacgc
      961 catcgcggcc gagtatcttc ccatgccgat agaagtcttc actgcttatt ttagggtcct
     1021 c
//
LOCUS       DATMKH010000660         1248 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_625734,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000660 DATMKH010000000
VERSION     DATMKH010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1248
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625734"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="VNV63_07840"
     CDS             <1..329
                     /locus_tag="VNV63_07840"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXC62570.1"
                     /translation="VLTVISDAVALIGGYAVAVFISRQSSPVYWSSASQAFNFQNLLG
                     GVIKPFVFGFIIAIVGSHYGLQTEGGTKGVGRYTTHAVVVASILILVSNFLITKLLIS
                     LYGWRT"
     gene            326..1057
                     /locus_tag="VNV63_07845"
     CDS             326..1057
                     /locus_tag="VNV63_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXC62571.1"
                     /translation="MIRLDQVSCSLGGHLVLKDISFDVQEGEILVILGASGSGKTTIL
                     RLILGLIRPDSGKIFVDGEEITQMSERKLMGVRQKIGMVFQEGALFDSLTVGENVAFS
                     LREQRTMSEEEIEERVRKILGFVDMSETIDMMPDELSGGMKRRVAIARALAAYDPKVM
                     LYDEPTTGLDPITAHTICELIVKLRDFQKVCSIMVTHQLKDAFKVATRFIVLGDCRII
                     FEGTGEDLKMSKNAYIQRFFQRSLR"
     gene            1054..>1248
                     /locus_tag="VNV63_07850"
     CDS             1054..>1248
                     /locus_tag="VNV63_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HXC62572.1"
                     /translation="MKRSVNITWAELKVGLVVMVGFVILSLAILSFGTIRNFFLPRVP
                     VEALFLNVKGLKQGAPVWLAG"
BASE COUNT          284 a          289 c          367 g          308 t
ORIGIN      
        1 ccgttcttac cgtgatcagc gatgcggtgg ccctgatagg agggtacgcc gtggcggtct
       61 tcatcagccg tcaaagctca ccggtttact ggtcttccgc gagccaggcc ttcaattttc
      121 agaacctcct cggcggtgtt atcaaaccct ttgtgttcgg cttcatcatc gcaattgtcg
      181 gctcgcatta cggacttcaa acggaaggag gaacaaaagg cgtgggccgc tacacaacgc
      241 atgcggtcgt ggtggcttcg atccttatac tggtgtcgaa tttcctgatc accaagctac
      301 tgattagcct ctatggttgg agaacatgat taggctcgat caggttagct gttctttggg
      361 aggccatctg gtcttgaagg acatttcatt tgatgttcag gagggcgaga tcctggtgat
      421 tctgggagcc agcggatcgg gtaagaccac gatcctccgg ctgatcttgg gactgatccg
      481 gcccgactcc ggcaagatct ttgtggatgg ggaagagatc acccaaatgt cggagcgaaa
      541 actcatgggg gtccggcaaa agatcgggat ggtgtttcag gaaggcgccc tttttgattc
      601 cttgaccgtg ggagaaaatg tagcgttctc cctccgggag cagagaacga tgtcggagga
      661 agagatcgag gagcgtgttc ggaaaatcct gggttttgtg gatatgtccg agacgatcga
      721 catgatgccg gatgagctca gcggcgggat gaaacggcga gtggcgattg cccgagcgct
      781 cgctgcttac gatccaaagg ttatgctgta cgatgaacct acaacgggac tggatccgat
      841 caccgcccac acaatctgtg aactgatcgt caaacttcgt gattttcaaa aggtctgctc
      901 gatcatggtg actcatcagt tgaaagacgc ctttaaagtg gcgacacggt ttattgtgct
      961 gggtgactgc cggatcatct ttgaagggac gggagaggat ctaaagatgt caaagaatgc
     1021 ctatatccaa agattctttc aaaggagcct cagatgaagc gaagtgtcaa cattacctgg
     1081 gccgagctca aagtgggatt ggtcgtgatg gtggggtttg tgatcttaag cctggccatc
     1141 ctttcgttcg gaacgatccg aaacttcttt cttccacggg ttccggtgga ggcgctcttt
     1201 ctaaatgtca aaggactcaa gcaaggcgca ccggtatggc tcgcaggt
//
LOCUS       DATMKH010000661         1221 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4769596, whole genome shotgun sequence.
ACCESSION   DATMKH010000661 DATMKH010000000
VERSION     DATMKH010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4769596"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="VNV63_07855"
     CDS             complement(<1..495)
                     /locus_tag="VNV63_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62573.1"
                     /translation="MENQSDRLIEIAETLKSMWLRPKRDIPATRKLLEEARELCRMQD
                     QNFDKLVRSGYFGIGKTQAYNILKGLSSSPKPKTSRVHRVNVIQENTVEQEDPVNPDD
                     TVEKKPDEFDEFMERLVNALGKAMDLPEVPDIVQQVFHAVGELSDKSADWMAYTLRAI
                     MLVDY"
     gene            585..923
                     /locus_tag="VNV63_07860"
     CDS             585..923
                     /locus_tag="VNV63_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62574.1"
                     /translation="MSIGIILGVKLMENPMTAAKVHEARVRRKLARMGVRLEKSHARD
                     PDHITYGTYQILRGNLIVHRGLDDGFGLSLPEVIEWIETHTTANGPVPADGGTPSFFI
                     FDRPTKEKTR"
     gene            920..>1221
                     /locus_tag="VNV63_07865"
     CDS             920..>1221
                     /locus_tag="VNV63_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62575.1"
                     /translation="MSMEFKIRFPRGYIAHPYWVERERVINITKKSGTNRAKSEKTRE
                     SSLLQYLESEGMTLADYEALVAMADRPFYTASDVERLSFGDFPLGDHDPQEITIPP"
BASE COUNT          270 a          304 c          324 g          323 t
ORIGIN      
        1 ataatcgacc agcattatcg cacgcaatgt ataagccatc caatctgcgg atttatcaga
       61 aagctcaccg actgcgtgaa atacttgctg cacaatatca ggcacttcgg gcaaatccat
      121 cgctttacca agcgcgttca cgagtctttc cataaattcg tcaaattcgt cgggtttctt
      181 ttctacagta tcgtctgggt taacagggtc ttcttgttct acagtatttt cttggataac
      241 gttcacccgg tgaacgcggc tggtttttgg cttggggctg ctcgacaacc cctttaatat
      301 attgtaggct tgggttttgc cgatgccaaa atacccgctc cgcacaagtt tgtcaaagtt
      361 ttggtcctgc atacggcaaa gctcgcgcgc ctcttctaag agctttcgcg tcgccgggat
      421 atcccgcttc ggccggagcc acatgctttt gagggtctcg gcgatctcga tgagccggtc
      481 ggattgattt tccattttcc aagtatggca cctttcggga tcaacgtcaa gcccatcttc
      541 ggggttaggg tgaaaataat tttgatcggg gagggttgac ctgggtgagc atcgggatta
      601 tcttgggggt gaaactcatg gagaacccga tgaccgcagc aaaagtccac gaggcgcgag
      661 ttcggcggaa gctggcccgg atgggggttc gcctagagaa gtcgcacgcc cgcgatcctg
      721 atcatatcac ctatgggacc tatcagatcc tacgcgggaa tcttattgtg catcgcggat
      781 tggatgatgg gtttggtctt tctctccctg aggtcatcga atggattgag acccatacga
      841 cggcgaacgg gccggttccg gctgatggtg gaacgccgag cttctttatc tttgatcgtc
      901 cgactaagga gaaaacgcga tgagtatgga gttcaagatt cgtttccctc gtggatacat
      961 tgctcaccct tattgggtgg aacgcgaacg tgtcatcaac atcacgaaga agtctggaac
     1021 caatcgggcg aaatcggaaa agacccgtga aagttccttg ctccaatatt tggaatccga
     1081 aggcatgact ctggcggatt atgaagcctt ggtcgccatg gctgatcgtc ccttctatac
     1141 ggcgagcgat gtcgaacgtt tgtcttttgg tgacttcccc ctgggggatc acgatcccca
     1201 agagatcacc attccgccga t
//
LOCUS       DATMKH010000662         3413 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2212502, whole genome shotgun sequence.
ACCESSION   DATMKH010000662 DATMKH010000000
VERSION     DATMKH010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3413
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2212502"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="VNV63_07870"
     CDS             complement(<1..421)
                     /locus_tag="VNV63_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HXC62576.1"
                     /translation="MSGRELDKIYQPKTVEERWYAFWLQQKIFHAEDKSSKPSFSIVI
                     PPPNITGSLHVGHALNNTLQDILIRWKRMSGFNTLWLPGTDHAGIATQNVVERQLIAE
                     GKSREVLGRKEFIERVWQWREQSGRTIIEQLKRLGASC"
     gene            complement(671..1636)
                     /locus_tag="VNV63_07875"
     CDS             complement(671..1636)
                     /locus_tag="VNV63_07875"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXC62577.1"
                     /translation="MAKRLMVVDSSLATQKLIEFSLGKEGFEVTSFSDGLSALDAVEK
                     VQPSLVVADYNLPGISIQRFCEKLKKQPGFHDRPILLMINYAETADREKLKQAGVTDF
                     IKKPVEVTNLLEKIKTFSKGTEDSAEPTVSMFRPEVAQSTKEKEEMMKIEELLGWSIP
                     PEQSFAGFAEPEKVETALEEKTIYGSPKEDIILVEEEHPPASPHASNELREPMLEIPL
                     PEPSLVTSAPEPPAATTPPAYATMFPHVSIPPEPVTQEIIEQVAWETVPGFAESAVQK
                     LTQELMQSVVNKLAKEIVEKVAWEVIPSLAEIAIQKEIEKLNKEE"
     gene            complement(1760..2137)
                     /locus_tag="VNV63_07880"
     CDS             complement(1760..2137)
                     /locus_tag="VNV63_07880"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HXC62578.1"
                     /translation="MKNRFQARSIKAMVAFAVFLTLAMFPTHRSNAVEGKGNPEAGKI
                     VFEKNCIGCHGKNGEGLGPMSKLPNFTDTKTMMARSDKDLFDKITNGGKGTGMPAWGP
                     LISEKDRWNLIAYIRTFASAPSR"
     gene            2256..2537
                     /locus_tag="VNV63_07885"
     CDS             2256..2537
                     /locus_tag="VNV63_07885"
                     /inference="COORDINATES: protein motif:HMM:NF016271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF507 family protein"
                     /protein_id="HXC62579.1"
                     /translation="MRLTKERIHQIANSMAEHLFSGEMLSTALSKSQVGTKIEQMITD
                     ELMIEDRLNDEVRELLKAYESQIEKGQVDYQKMFQMIKRKLAKEREIIL"
     gene            2539..2817
                     /locus_tag="VNV63_07890"
     CDS             2539..2817
                     /locus_tag="VNV63_07890"
                     /inference="COORDINATES: protein motif:HMM:NF016271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF507 family protein"
                     /protein_id="HXC62580.1"
                     /translation="MSLSEEKISHLAHRILDGLSEDLDLKWQIEEEKVLREIKKVIVA
                     ELGLEEEMDRLVRHRLASYSRSIAEGSPEWEVLYRKFFNEELRKRKKA"
     gene            complement(2814..>3413)
                     /locus_tag="VNV63_07895"
     CDS             complement(2814..>3413)
                     /locus_tag="VNV63_07895"
                     /inference="COORDINATES: protein motif:HMM:NF013275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HXC62581.1"
                     /translation="QGLADEFKKALGNRAEVVYYDGLVQGDKDFRGVLTTVASKKPDV
                     FFFGGIYPEGGLLVKQAKEVGLTAPMVSGDGVIDQVFVQIAGAAADGTYLTFSPDTTK
                     LLSAQEVLKKYRARYGEPGPYSFYAYDAAQVLLLGIRKAGTPDGFAVSQAIHTSSYDG
                     ITGRIQFDSKGDLTETQYVVWITKGGQFEEFWKPAKESL"
BASE COUNT          792 a          846 c          910 g          865 t
ORIGIN      
        1 cacaagaggc accgaggcgc ttgagttgtt cgataatcgt ccggcctgat tgttccctcc
       61 actgccatac ccgttcgatg aattcctttc tgcccagcac ctcccgactt ttaccttccg
      121 ctatgagttg ccgttccacc acattctgcg tggcgatacc tgcgtggtcg gtgcccggca
      181 gccagagggt gttgaaccct gacatccgct tccatcggat gaggatatcc tgaagtgtgt
      241 tatttagggc gtgccctacg tgaagtgagc cggtaatgtt gggtggcggg atgacgattg
      301 aaaaagacgg tttgctgctt ttgtcctcgg catgaaagat cttttgctga agccagaaag
      361 cgtaccagcg ctcttcaaca gtcttcggct gataaatttt atccagttct cgtccagaca
      421 tcgggactac atcctaacat gagtttctcg gagaggcaag ggcttgacat ttaaacggtt
      481 gcggagtatg tataatcacc tactaaggag gaatcggatg ggacatggac gacagataga
      541 tcgagagctg aggcgaaaac accgaaaagg tgaagagagg cttcgagcaa aacggaaaac
      601 gttgagaatg gccaaaaagg ctgagcagcc tgcgaccaag gccaagaaat cctgatatca
      661 gcctttaaaa ttactcttct ttatttaatt tttcaatttc tttttgtatc gcgatctcgg
      721 ccaggctggg gatgacttcc caggccacct tttcaacaat ttccttggcc agtttattga
      781 ccacgctctg catcagctcc tgagtgagtt tctggacggc ggactcggcg aagccgggaa
      841 cagtctccca tgcgacttgt tctatgatct cctgggttac cggctcgggg ggaatcgaaa
      901 cgtgggggaa catcgttgcg taggcgggtg gagttgttgc cgcgggaggc tccggagccg
      961 acgtaaccag ggatggttct ggaagcggga tctccagcat gggctcacgg agttcattgg
     1021 aggcatgggg agatgcgggt ggatgttctt cctctaccag gatgatgtct tctttcggag
     1081 aaccatagat cgttttctcc tccagcgccg tctctacctt ttcaggctcc gcaaaacctg
     1141 cgaatgactg ctccggaggg atggaccagc ccagcagctc ctcgatcttc atcatttctt
     1201 ctttttcctt ggtgctctgt gcgacttctg gtctgaacat ggataccgta ggttcggccg
     1261 agtcctccgt tcctttagaa aatgttttta tcttctccaa aaggttggtg acctctacag
     1321 gttttttgat gaagtcggtt acgcctgcct gtttgagctt ttcgcgatca gccgtttccg
     1381 cataattgat catcagcagg atgggccggt catgaaaacc cggctgtttc tttagcttct
     1441 cgcaaaagcg ttggatgctg atgcctggaa gattgtagtc ggccacgacg agagagggtt
     1501 gaaccttctc cacagcatcc agtgccgaga gcccatcgct gaaggaggtt acctcaaaac
     1561 cttctttacc caagctaaac tctatcagct tctgggtagc gagactgcta tccaccacca
     1621 ttaaccgctt ggccattgac gaatccttgt aacaatattt atattgggaa cttttttgac
     1681 cgtatcatag gggatctgat ttgtcaagaa aagcaccgtt cttaagagct tgcttgcgga
     1741 gtttctcagg cgagaccttt tatcttgacg gtgcagaggc aaaggtccga atataggcga
     1801 tgagattcca gcgatccttt tcgcttatca gggggcccca ggccggcatg ccggttcctt
     1861 ttccgccatt ggtgatctta tcaaacagat ccttatcgct ccgagccatc atcgtcttgg
     1921 tgtctgtgaa gttcggcagc ttagacatag ggccgagtcc ttctccgttt ttaccgtgac
     1981 agccgataca gtttttttca aacacaattt tgccggcctc ggggtttccc ttgccttcta
     2041 ccgcattgct tctatgggtg ggaaacatcg ccagggtgag aaagacggca aaagcgacca
     2101 tcgctttgat ggatcgggcc tgaaagcggt ttttcatcgg gcaactcctt atagagtatg
     2161 cagtataacg aggtctccag ggcctgtcaa gctgacgcat tgacaggtcg ttgatgtttt
     2221 gttaaagtac tcaatccgga tcctagaaga agtatatgcg attgacaaaa gaacggattc
     2281 atcagatcgc caacagcatg gccgagcatc tcttctccgg agagatgcta tcgaccgcgc
     2341 tttccaaaag tcaagtcggt acaaaaattg agcagatgat tacggatgag ctgatgatcg
     2401 aggaccgtct gaacgatgag gtgcgtgagc tgctgaaggc atacgagtcc cagatcgaaa
     2461 aaggtcaggt ggattatcaa aagatgtttc agatgatcaa acgaaaattg gccaaggaac
     2521 gggagatcat tctgtagcat gtcgctcagt gaagagaaga tcagccacct tgcgcatcgg
     2581 attctggatg gattgagcga ggatctcgat ctcaagtggc aaatagagga ggagaaagtc
     2641 ctacgcgaga tcaagaaggt gatcgtggcc gagctcggac ttgaagagga aatggatcgc
     2701 cttgttcggc accgcttggc ctcctattct cggtcgatcg cggaggggag cccggagtgg
     2761 gaggtgctgt accggaaatt ttttaacgag gagttacgaa aacgaaagaa agcttagagt
     2821 gattctttgg caggtttcca aaactcttcg aattgcccgc ccttcgtaat ccagacgacg
     2881 tactgagtct ctgtaagatc ccctttcgaa tcgaactgaa tccggccggt gatcccatcg
     2941 tagcttgatg tgtggatcgc ctggctgact gcaaacccgt ccggcgtgcc ggccttccgg
     3001 attccaagaa gcagcacctg agcggcgtca taggcataaa atgaatacgg acccggttca
     3061 ccgtaccgtg cacggtattt cttgagaacc tcctgggccg agagtagttt ggtcgtgtcc
     3121 ggactgaagg tcaggtaagt tccgtcggcc gctgcgccag cgatctgcac aaaaacctgg
     3181 tcaatgacgc catcgccgct caccatcgga gcggttaagc ctacttcttt ggcctgtttg
     3241 accaggagac cgccctccgg ataaattccg ccgaaaaaaa agacgtccgg tttcttggaa
     3301 gccacggtcg tgaggacacc ccggaagtct ttatctccct ggacgaggcc gtcatagtat
     3361 acgacctccg cacggtttcc cagggctttc ttaaactcat ccgccagacc ctg
//
LOCUS       DATMKH010000663         1406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4476191, whole genome shotgun sequence.
ACCESSION   DATMKH010000663 DATMKH010000000
VERSION     DATMKH010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1406
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4476191"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(747..968)
                     /locus_tag="VNV63_07900"
     CDS             complement(747..968)
                     /locus_tag="VNV63_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008104944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HXC62582.1"
                     /translation="MATIAEVEKLALDLPETERAVLAAHLLGSLPSVLHDEDEGIAEG
                     LRRDAEFDATPSLGMSLEELDQRIQSRRS"
BASE COUNT          346 a          405 c          338 g          317 t
ORIGIN      
        1 gggtttgcat cccggctaca ggttagcgac agagaccgtc tggtatgttt gcgtctgaaa
       61 tctctgactc gatcattaat gccctgactc aaaaccgcgt tatcctggga ttcttcagat
      121 tcccgtggag aggcgacaat catcaaaaca catagaaaat ttgtacgtag atcgaccaca
      181 atttaccaca catttgcttc cgctcacttc cacaggtttc caccctgatg gaaatttccc
      241 ggggaaatag atggaaaata gcggaaaaca gaaccctaaa atggggttca agtccccccc
      301 cgagcagaga tgcccacctt taagtggttg ttttcgaaga acttaggttc taagacgctt
      361 ggacagattc agaatctcga cccccaccgc ctggtgttcc gattaaaatc caagagcact
      421 ccaggcgaca ctcttcgtac tcggctatac tcctatcgac gtcaggatcc aagagatgga
      481 tatcgatggt gatggcggac gaccagaatt cggacttcgt gggcaacgcc gcggaacaga
      541 aagtgataag ggaaccgccg caaattcgct ctccggatat cacgctcccg gattgaaaaa
      601 gatggtggct tgtctgcgac ctgggtcacg aaataacgga gctcggtgta aaactcctct
      661 ccgagcccct ggagtcgcaa cgcgctcgta gtagcccatt atctcgtcaa tgtccgaata
      721 gaccttcgga tgaaggacaa gccgcatcag cttcgacggc tttggatccg ttggtcgagt
      781 tcctctaatg acatgccaag ggatggagtc gcatcaaatt ccgcatcgcg acgcagacct
      841 tctgcgatac cttcatcttc gtcgtgcagg accgatggaa gcgagcccag taggtgtgcc
      901 gccaggacag ctctctcggt ctctggtaaa tccagtgcca gtttctcgac ttctgctatg
      961 gtcgccatcg gagcaagtct agtttcaacg gcctcgaagg caagggatca tcatgaaacc
     1021 gcaacctaaa tacaccgcgg tctgatgcca cctcagccgg ttctcaaagt tcgctcggac
     1081 gcggaagatc agcaattaaa atctgtcctc tgcgccttca gccaggtagc ccacaacttc
     1141 gaccgatctc gatcatcccg tctctcgtgg cgtttggttc ctcgtccttg aacaaagtca
     1201 ggctggaata ctcggcggcc acctgactgc acaattcggc cgcagaagta agcacatttg
     1261 attcggacac ctactaccct aactttgtga aggcacctcg cgggttcccc atcccctgta
     1321 ttggaaaaga gcattcccca gcaagaccgc cccaaccttc cccttctctc taggacgccg
     1381 gcgggcgact tgaccgaaca gaacct
//
LOCUS       DATMKH010000664         1848 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2536913, whole genome shotgun sequence.
ACCESSION   DATMKH010000664 DATMKH010000000
VERSION     DATMKH010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2536913"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="VNV63_07905"
     CDS             complement(<1..591)
                     /locus_tag="VNV63_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62583.1"
                     /translation="MALSNGIENVWQEGKAREADPAPFTEKDLQATISSRVRKEFKTV
                     SQFVWAAIVYQIILYSFLTRTFIRHWGDMQIMLLCLAGGACYIPLTVALLRRVKTLYL
                     RPSEAPGSPVLDVFHKVEGEYARLADFFQFKKRMDWIGVPVSCAIIVLVTFTLFVQGG
                     VEGNPRASLAIFALWVGMSLIAIQAENRKRFISPLRH"
     gene            complement(607..1110)
                     /locus_tag="VNV63_07910"
     CDS             complement(607..1110)
                     /locus_tag="VNV63_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018478796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXC62584.1"
                     /translation="MHAEPEASFLANLNQNIRIVHRVCHTYFPRDAMEREDVFQDIMY
                     QLWKSYPHFHWESKFSTWMYKVALNTAITHIRRSTRAPRNTELTESVAHAPDINEQVS
                     REEELHRLHEAIAALTDIDKAIILLHLEDQNYDEIASITGLTRTNVSVRLVRIKRALK
                     DDLQGNQ"
     gene            complement(1420..1698)
                     /locus_tag="VNV63_07915"
     CDS             complement(1420..1698)
                     /locus_tag="VNV63_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62585.1"
                     /translation="MLEFQHFGRFVDIFFGALIASGLERRIDRRSRSDCVCLGKGDSV
                     PEIEILPKVKPMILAENHPKVRAVFPHTSSLQTVTSVGRDFSGLMISN"
BASE COUNT          427 a          501 c          489 g          431 t
ORIGIN      
        1 atgccggagc ggagaaatga accgtttcct attttcagcc tggatggcaa tgagggacat
       61 cccaacccag agggcgaaga tggcaaggct tgcccgagga ttcccttcaa cgccaccctg
      121 gacgaagagt gtgaacgtga ccagtacaat gatcgcgcag ctgacgggaa caccaatcca
      181 gtccatgcgc ttcttgaact gaaagaaatc ggcaagccgg gcgtactcac cctccacttt
      241 gtggaagaca tccaggaccg gagacccagg cgcttccgag gggcggaggt acaacgtttt
      301 cactctgcgt aggagagcga cggtcagcgg gatgtagcac gctcctccgg cgagacagag
      361 gagcatgatc tgcatgtcgc cccaatggcg gatgaacgtg cgcgtgagga acgagtagag
      421 aatgatctga tacacaatgg cagcccagac gaactggcta actgtcttga actcttttct
      481 gacgcgcgac gagatagtcg cctgaaggtc cttttcggtg aatggagcag ggtccgcttc
      541 acgggctttc ccttcctgcc atacattctc tattccattg ctgagtgcca tagtccctcc
      601 tctgaattat tgatttcctt gaagatcgtc tttcagcgcc cgcttgattc gtaccaggcg
      661 aacactcacg tttgttctgg tgagacccgt gatggaggca atttcatcgt aattctgatc
      721 ctccagatga agcagaatga tcgccttgtc gatgtccgtt aacgcagcga ttgcttcatg
      781 caaccgatgg agttcctctt cccggctcac ctgctcgttt atgtcgggag catgggcaac
      841 cgactcagtc aactctgtat ttcgaggcgc ccgggtgctc cgccgaatgt gggtgatcgc
      901 cgtgttcagc gcgactttgt acatccatgt ggagaatttt gattcccaat ggaagtgcgg
      961 ataggacttc cagagttgat acatgatgtc ctggaagaca tcttcgcgtt ccatggcatc
     1021 gcgggggaaa taggtatggc aaacccggtg gacaatcctg atgttctgat tcaggtttgc
     1081 gaggaacgat gcttccggct ctgcgtgcat tctgccatcc tttgtccgtg cattgtatga
     1141 gtagccggcg aggaacaaaa actacacgtc tggcaaggat ttggggaaga aataacggaa
     1201 gaagcgtccg ggctccagcc gcccccctgc gaggttacgc gaagtgtacc cgagcggcct
     1261 gtgcagcccg gagaaccccc agaattgggc cttgttcctg ataaacgcag ctgtttccgg
     1321 accggtagac cggccccaaa accccctgtc cggtaagcgg acctatcagg agacatcggt
     1381 agcggttatt cttagtgctt tcccaaaaaa tcgatcggct caatttgata tcatcaagcc
     1441 actaaagtcc ctgcccacac ttgtgaccgt ttggaggcta ctcgtatgtg ggaagacagc
     1501 tcttactttt gggtggttct ctgcaagaat cattggtttc accttcggaa gaatctctat
     1561 ttccggcaca gaatccccct tgccgagaca gacgcaatca ctcctcgacc tcctgtcgat
     1621 tctcctttca aggcccgatg cgatgagtgc gccaaagaat atgtctacaa accttccgaa
     1681 gtgctgaaat tcgagcaaga acctcccgaa tcattcactc cgcatcccca atttagtgag
     1741 gaaatcagtg gaacccatcc ggttgccgcg gataagtctc cagacgacac aggacactag
     1801 acgtcgcctc ctaaatggtt ttcagggggt cggagcttta gctccgac
//
LOCUS       DATMKH010000665         1626 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_946493,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000665 DATMKH010000000
VERSION     DATMKH010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_946493"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="VNV63_07920"
     CDS             complement(<1..618)
                     /locus_tag="VNV63_07920"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HXC62586.1"
                     /translation="MKDQKKDESFKEFIVEAEEILDSLNQNLLTLESAKDKTSVPPDV
                     INSIFRAAHTLKGMSGMVGLKKISELSHRLEDLLDRLRMGKLQLSSLVTDTLFAGADG
                     LRKLIETVSTGRGEKFEVGPVLKKIEQALRGEQPHAARPSPNREAERPPVESNPLEDA
                     GVPVELVKVLTEYESHRLLESIKSGRHLYDVHARFKLETFDKDLTR"
     gene            complement(651..1022)
                     /locus_tag="VNV63_07925"
     CDS             complement(651..1022)
                     /locus_tag="VNV63_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015350754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXC62587.1"
                     /translation="MAKKILIIEDSATMRSLIASTIEEMSGFELVEASNGFEALKALP
                     SQHFDMIITDLNMPDINGLEIIKFVKEHPAYKSIPLVVVTTEVGEEDRKKGLALGASD
                     YVIKPFEPDDLKKMVRKILKH"
     gene            complement(1028..>1626)
                     /locus_tag="VNV63_07930"
     CDS             complement(1028..>1626)
                     /locus_tag="VNV63_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62588.1"
                     /translation="APGLKMDPEKTQSRRTVAQKPAPAPIGGQRNTKAVRQDLRLLTI
                     MIEELSRPSTSSEVTLLILRFASEIMNRAVIFLTRREDILGLGQSGLVLPGGGNANER
                     IRNLRIPLAEESIFKFVMGKKLPYKGKLEINHWNEFLVERLGREWPVEVYVTPLINEG
                     RVIAILYGDNLPKQEAIGETEGLETFVKVAAFKLQKPE"
BASE COUNT          379 a          438 c          367 g          442 t
ORIGIN      
        1 gcgcgtcaga tccttgtcga aggtctcgag cttaaaacgc gcatggacat catagaggtg
       61 tcgcccagac tttatacttt caagaaggcg gtgactttca tattcggtca agaccttcac
      121 cagctcgacg ggcaccccgg catcctccaa cggatttgat tcaacaggag gacgctcagc
      181 ttctcggttc ggagacggcc gggcagcgtg gggctgctct cccctcagtg cctgttcaat
      241 ctttttgaga acaggtccaa cctcgaattt ttctccacgt cctgtgctta cggtttcgat
      301 aagttttcgg agcccatctg caccggcaaa caaggtgtcc gtaacaagag agctcaattg
      361 gagtttaccc atacgcaatc ggtccagcag gtcttccaaa cggtggctga gctccgagat
      421 ctttttgagt cccaccattc cggacattcc cttcaaggta tgggccgcac ggaagatcga
      481 gttgattaca tcgggaggca cggaagtctt atctttggca gactccaggg tcaagaggtt
      541 ctgattcaaa ctgtccagga tctcctctgc ctcaacgata aactctttaa aggactcatc
      601 ttttttttgg tctttcattc gcgatcacac ttttgaaaat gataacgcct ttaatgcttc
      661 aaaattttac gaaccatctt cttcagatca tccggctcaa atggtttgat aacgtagtca
      721 gaagctccca acgccaatcc cttctttcga tcctcttctc caacttccgt cgtgacaacc
      781 acgagaggaa tgcttttata agcggggtgc tccttaacaa atttaataat ttcgagtccg
      841 ttgatatccg gcatgttgag atccgtaata atcatatcaa aatgctgaga gggaagcgcc
      901 ttcaaggctt caaatccatt gctggcctcg accagctcaa aaccactcat ttcttcaatc
      961 gtggatgcaa tcagcgaccg catcgtcgca gaatcctcga tgatcaaaat cttcttggcc
     1021 attaaaatta ttccggtttc tgcaacttaa aggctgccac cttgacaaac gtttctaacc
     1081 cttctgtctc gccgatggcc tcctgcttgg ggagattgtc gccatagagg atcgcgatga
     1141 cgcgaccctc gttgatcaaa ggtgtgacat acacctcaac cggccattct cttccaagcc
     1201 gttcaacaag aaactcgttc cagtggttta tttctagttt tcctttatac gggagttttt
     1261 tccccatcac gaatttaaat atggactctt ccgccagggg gattcgaagg tttcggatcc
     1321 gctcgttggc attgccgcct ccaggtaaga cgagaccgga ctggcccagc cccaagatat
     1381 cttcccttcg tgtaagaaag atcacggcac gattcatgat ctcgcttgca aatcgcaaaa
     1441 tgagcagtgt aacttcactg ctcgtggaag gcctggaaag ctcttcaatc atgatcgtga
     1501 gcagccggag gtcttgccgg accgcttttg tatttctctg accgcctatc ggtgcaggag
     1561 ccggcttctg agccacggtc cttctggact gagtcttctc cggatccatt ttgagtccag
     1621 gggctg
//
LOCUS       DATMKH010000666         1100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4772036, whole genome shotgun sequence.
ACCESSION   DATMKH010000666 DATMKH010000000
VERSION     DATMKH010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4772036"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..744
                     /locus_tag="VNV63_07935"
     CDS             73..744
                     /locus_tag="VNV63_07935"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HXC62589.1"
                     /translation="MSKPKIKRSSEKIVRVDPVHPQPELLAKAADLIHRGGVIAFPTE
                     TFYGLGADALNRQAIERIYAIKGRDRSKPILILVEARDRINAFVKPFPSMAERLMTAF
                     WPGPLTILFQANDRLPIELTAGTGKIGIRFPSHPVVRALLSAVGVPLTGTSANRADGR
                     SPQTAEEVRNSIGLELDLILDGGSTPGEKPSTVLDCTTSPFQIVREGIVSRGDLETVI
                     GKIKG"
     gene            749..>1100
                     /locus_tag="VNV63_07940"
     CDS             749..>1100
                     /locus_tag="VNV63_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62590.1"
                     /translation="MQIRLTKLNQFFHYQKLNTKLLLMMFLLLLLSLMSSFFLYSFFE
                     TAMVKEVEDNTTDLSKAIQVSVEELTSTGETDEARLREYVDTLSKKGVKEISILSNEN
                     EVIASSNPKHKGAHL"
BASE COUNT          295 a          267 c          288 g          250 t
ORIGIN      
        1 tccagattct ggccaaacga taccctgcgt taaaggaaaa gctccgacga tataaacgcc
       61 aactggccca agatgtcgaa gccaaagata aaaagatcca gcgaaaaaat cgtacgagtt
      121 gatccggttc atccccaacc cgaacttctt gccaaagcgg ccgacctgat tcatcgaggc
      181 ggtgtcatcg cttttcccac cgagaccttc tatggcttag gcgcggatgc cttaaatcgt
      241 caagcgattg aacgaatcta tgcgatcaaa ggcagagacc ggagcaagcc gatcttgatc
      301 cttgtagagg cacgtgatag aataaatgcg ttcgtaaaac cgtttccttc gatggctgaa
      361 cggctcatga cggctttttg gccggggccc ttgacgatac ttttccaggc caatgaccgc
      421 ctccccatcg aattgacggc agggaccggc aaaataggca ttcggttccc ttcgcatccg
      481 gtagtaaggg cgcttctttc cgctgtgggg gttcctctga ccggtacgag tgccaatcgg
      541 gcggatggac gatctccgca gaccgcggaa gaagtacgaa atagtatcgg tctggaactg
      601 gacctgattt tggatggagg gtctacaccg ggtgaaaaac catccaccgt tctggactgc
      661 accaccagcc cgtttcagat tgtccgggaa gggatcgtat cgaggggaga cttggagaca
      721 gtgattggaa agataaaagg ataaagagat gcagatccgg ctgactaagc tcaatcaatt
      781 tttccattat cagaagttga ataccaagct tctcctgatg atgttcctgc ttctgctctt
      841 atcactcatg tcttcctttt tcctttactc cttttttgaa acggcgatgg tcaaggaggt
      901 agaagataat acgacggatc tatcgaaggc gatccaggtc agtgtcgaag agctcaccag
      961 cacgggtgag acagacgagg caagactccg agagtatgtg gatactctaa gcaaaaaagg
     1021 ggtcaaggag atctcaattc ttagcaatga gaatgaggtg atcgcgagtt cgaatccgaa
     1081 gcacaaaggg gcccatctcg
//
LOCUS       DATMKH010000667         2132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4137933, whole genome shotgun sequence.
ACCESSION   DATMKH010000667 DATMKH010000000
VERSION     DATMKH010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4137933"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="VNV63_07945"
     CDS             complement(<1..371)
                     /locus_tag="VNV63_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62591.1"
                     /translation="MRSSKWVLLTLSLVIAMAVSAQETKLEIAGSEIDVVFNSPVSDS
                     LREMVLARIRLSAKAIVNYYRRYPAREVRIDVFPNFGRGVSGGRASGWNGARIRLSVG
                     KESTPADFSEDWVTTHEMVHLA"
     gene            complement(629..1573)
                     /locus_tag="VNV63_07950"
     CDS             complement(629..1573)
                     /locus_tag="VNV63_07950"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal binding protein"
                     /protein_id="HXC62592.1"
                     /translation="MKLFYLNPTYPELDLEENVLKGTGIELVRYKVNSDADIASGADE
                     IDAILTDSTPITRQTVSYLKRCKIVVRLGVGFDVVDGAACRERGIVVCNVPDYGVEEV
                     ANHAIGLLFTVHRRILSYDRKVRHKGWGYQTEWPIRRLSKQKVGVIGIGRIGAQFASR
                     IKPFVHEVAAYDPGLNFAEIEKRGAVPQDLQPLLQSADLISLHLPLSERTHHLINQET
                     IGWMQRQPILINVSRGGLIDSAALAAGLRERKLSGAGLDVFEHEPDVPSEYLDLPNVV
                     LSPHIAWYSEEASQQLRTSAVRTIADYLTGKPAVNVVN"
     gene            1619..>2132
                     /locus_tag="VNV63_07955"
     CDS             1619..>2132
                     /locus_tag="VNV63_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HXC62593.1"
                     /translation="MSLREYARKRNFEQTSEPSDRSPVRKSKKPRFVIQKHAASRLHY
                     DFRLEMDGTLKSWAVPKGIPLKKGEKRLAVHVEDHPLAYADFEGTIPKGQYGGGTVMV
                     WDYGTYELSDRYPNKTLESGQLHVVLHGKKLEGEWGLVRLKSDKDEWLLIKAGSDANP
                     ISEELDDTSAL"
BASE COUNT          529 a          595 c          547 g          461 t
ORIGIN      
        1 gcgagatgca ccatctcatg ggttgtcacc cagtcctcag aaaaatcagc cggcgtactc
       61 tccttaccaa cgctgagcct gattcgcgct ccgttccaac cggaagcgcg gccgccgctc
      121 acccctcgcc cgaaattcgg aaagacatcg atccgcactt ctcgggccgg ataccgacga
      181 tagtagttaa cgatcgcttt ggccgagagc ctgatcctag cgagaaccat ctcccgcaga
      241 gaatcggaca ccggtgagtt gaagacgaca tcgatctccg agccggcgat ctcgagtttg
      301 gtctcttgag cggaaacggc catagcgatg acgaggctaa gggtgagcaa gacccatttg
      361 gaagaacgca tcattggagg tgaaaagtga aaggtgaaaa gtgaaaggtc ggccttgggt
      421 cgagcgagac tccagaacta aggaacttcc atgcgggtcc gatattcccg agaaatgcga
      481 acgtaggccc aaagatgccc ggtcaggggg agcaaattca ccatggaaac gttcccggga
      541 gccgcttgct gccgacctac ataactaacg gcttacaaag cggctcccct ccatggcgcc
      601 tttcaccttt cacctctcac ttctcacatc agttcaccac gttaaccgcc ggtttcccgg
      661 tcaaatagtc tgcgatcgta cgaaccgcac tggtccggag ctgctgactt gcttcctctg
      721 aataccaggc aatgtgaggg cttaagacca cgttaggtaa gtcgagatac tcgctgggca
      781 catccggctc gtgttcgaat acatccaacc ctgctcctga taacttccgt tcccggagac
      841 ccgctgccag agcagcgcta tcgatcaggc cacctcggct gacgttgatc agaatgggct
      901 gacgctgcat ccaaccgatc gtttcctgat tgataaggtg atgcgtccgt tcactgagtg
      961 ggagatgtaa actgatcaga tcagcgcttt gcagaagggg ctgcagatct tgagggacgg
     1021 cgccccgttt ttcgatttct gcaaaattga ggcccggatc gtaagcggcg acttcatgca
     1081 cgaaaggctt aattcggctg gcaaattggg cgccgatccg gccaatcccg ataacaccga
     1141 ccttttgttt gcttaaccgt cggatgggcc attccgtctg ataaccccat cccttatgcc
     1201 tgactttgcg atcataggaa agaattcgtc tatggaccgt aaacaaaagc ccgatggcat
     1261 gattcgctac ttcctccacc ccatagtctg ggacattgca aaccacgata ccgcgttccc
     1321 ggcacgccgc gccgtcaacc acgtcgaaac cgacccccag ccggaccaca attttacagc
     1381 gttttagata ggagaccgtt tgcctggtga tcggcgtgct gtcggtcaaa atcgcatcga
     1441 tctcgtcagc cccggatgca atgtcggcat cgctattaac tttatatcgg acgagctcaa
     1501 tcccagtccc tttaagtacg ttttcctcga gatcgagctc cggataagtc ggattcagat
     1561 agaaaagctt catcgcagtt taataatcgt ctataagttg agagatactg ctcaacttat
     1621 gtctctgcgg gaatacgcaa gaaaacggaa tttcgagcaa acatccgaac ctagtgatcg
     1681 ttcgccggtc cgaaagtcga agaagccgcg gtttgtcatc cagaaacatg cggcctcccg
     1741 cctgcattat gatttcagac tggagatgga tggaaccctg aaatcctggg cagtaccaaa
     1801 aggtattccc ctcaaaaagg gagaaaaacg actcgctgtc cacgtggaag atcatcccct
     1861 ggcgtatgcc gacttcgagg gaaccatccc gaaaggccaa tatggcggcg gtaccgtgat
     1921 ggtctgggac tacggtacct atgaactttc ggatcggtat ccaaacaaaa ccctggagag
     1981 cggccagcta catgtcgtcc tgcacggcaa gaaactagaa ggcgaatggg gtctggtacg
     2041 gttgaaatcg gacaaggacg aatggctact gatcaaagcc ggctctgacg ccaacccgat
     2101 ttccgaagaa ttggatgata cctcggctct tt
//
LOCUS       DATMKH010000668         1561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3499290, whole genome shotgun sequence.
ACCESSION   DATMKH010000668 DATMKH010000000
VERSION     DATMKH010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3499290"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1254)
                     /locus_tag="VNV63_07960"
     CDS             complement(<1..1254)
                     /locus_tag="VNV63_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HXC62594.1"
                     /translation="MLKKIYHFLDKRLHLTSLVRNALDEPIPGGASWIYVFGSVVLFL
                     FGLQLITGMFLVLYYVPTPDHAYDSINFVETEVAFGWFIRGIHHWGASCMMVAIGLHM
                     LQTFLYGAYRPPREVLWNVGVFLFLLVLTFAFTGYLLPWDQKAYWATSVGINMVGTIP
                     FIGGYLVRILRGGEDVGALTLSRFFAVHILFLPLFTVVLIALHLFILRRVGPAGPWDK
                     ERGQAIKEPFFPRQVFMDAVIMFVTFGIVVAMAVWLPFPLSDKANPSDTSFVPVPEWY
                     FLFYYQLLKYFDGPLQPIATIVLPLLFYALLFLLPYLERGKERNPGRRPTVIVTGAVF
                     LILVFFLLGISIGQISAVAVKNPSVAAGKDIYQRLQCSGCHRIHGTGGAVGPDLSFVG
                     DVRDADWLTRHFKDPQAVVPGSIMPK"
     gene            complement(1270..>1561)
                     /locus_tag="VNV63_07965"
     CDS             complement(1270..>1561)
                     /locus_tag="VNV63_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HXC62595.1"
                     /translation="WLKTSITQWVWAVRHSDEEITVYSPICTHLGCGYHWDPGEGQFA
                     CPCHGSIFSIDGKVLAGPAPRPLDTLPVKIENGRLFITYEQFKSGTKEKVAI"
BASE COUNT          438 a          418 c          424 g          281 t
ORIGIN      
        1 cttcggcatg atagagccgg gaacaactgc ctgcggatcc ttaaaatgcc gggtcagcca
       61 gtccgcatca cgcacatctc ccacaaagga caggtccggg ccgaccgctc cccccgtccc
      121 atggattcga tggcagccgg agcactggag tctctggtag atgtcttttc cagccgcgac
      181 agagggattt ttcaccgcta cggccgagat ctggccgatc gagattccaa ggagaaaaaa
      241 aacaagaatt agaaatacgg ctcctgtcac tatcacagta ggccggcggc ctgggttcct
      301 ctccttccca cgctcgagat atggaaggag aaacagaagg gcatagaata ggagcggcaa
      361 cacaatcgta gcgatcggct gaagcggccc atcaaaatac ttgaggagct ggtagtaaaa
      421 gagaaaatac cactccggga ccggaacaaa gcttgtgtcg gatgggttcg ctttgtctga
      481 gagaggaaag ggcagccaga ccgccatcgc aaccactata ccgaaggtaa cgaacatgat
      541 gaccgcatcc ataaagacct ggcgtgggaa aaagggttct ttgatcgctt ggcctcgctc
      601 cttatcccac ggcccggcag gcccgacccg ccgaaggatg aagagatgaa gagcgatgag
      661 aacaaccgtg aacaggggca gaaacagaat atgaaccgcg aagaaacggc tcagtgtcag
      721 cgccccgaca tcttctccac cccggaggat ccggaccagg taacctccga taaatggaat
      781 ggtccccacc atattgattc caacgctggt tgcccagtaa gccttctggt cccaaggcag
      841 gagatacccg gtgaaggcga aagtcaggac caggaggaac aaaaataccc ctacgttcca
      901 aagcacttcc cgcggaggac gataagcccc gtagagaaag gtctgaagca tgtggaggcc
      961 aatggccacc atcatgcagc ttgcccccca atgatgaatg ccccggataa accaaccgaa
     1021 ggcaacctcg gtctcgacaa aattgatgct gtcgtaggcg tgatccggcg tgggaacgta
     1081 gtacaggaca agaaacatcc cggtaatcaa ctgaagaccg aagagaaaga ggacgacact
     1141 gccgaagacg taaatccagg atgcccctcc cggaatcggc tcatccagag cattccggac
     1201 aagggatgtg agatgaagac gtttatccag aaaatggtag atctttttca gcaccgagac
     1261 ctggaatcct cagattgcga ccttctcctt cgtcccagac ttgaactgtt cgtaagtgat
     1321 aaaaagacgg ccattttcga tcttgacagg gagggtatca agaggccgag gagcaggacc
     1381 ggccaggact ttgccgtcaa tagaaaaaat actcccgtgg cagggacagg caaattgccc
     1441 ttcacccgga tcccaatggt atccgcagcc taaatgtgtg cagatcggag agtagactgt
     1501 gatttcttca tcactatggc ggacggccca gacccattgt gtgatggagg tcttgagcca
     1561 c
//
LOCUS       DATMKH010000669         1486 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1897528, whole genome shotgun sequence.
ACCESSION   DATMKH010000669 DATMKH010000000
VERSION     DATMKH010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1486
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1897528"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="VNV63_07970"
     CDS             <1..256
                     /locus_tag="VNV63_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62596.1"
                     /translation="LEVAFREVRAAAFVAEGEKPMGEGRAVVIAGPEAIAAFPRENTR
                     IGAQTRVFTGLTSRIYSPRQGRGSIAENLSCGFILFWLRA"
     gene            complement(231..509)
                     /locus_tag="VNV63_07975"
     CDS             complement(231..509)
                     /locus_tag="VNV63_07975"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HXC62597.1"
                     /translation="MYSVILSNQSIKNLTGIVEYIAAENPDAADKIGFELVNLAMSLD
                     FMPYRGSKVKKRLGVLKLVHGNYLLYYRVDEGKRTVQVISFKHGAKIK"
     gene            complement(519..752)
                     /locus_tag="VNV63_07980"
     CDS             complement(519..752)
                     /locus_tag="VNV63_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62598.1"
                     /translation="MSIKEILEELPKLTQEEKKQLWNALNHEAATYDVEEESPELLAA
                     IDEGICSLESGERTYTIEEARQLVAETVAKARR"
     gene            complement(1195..>1486)
                     /locus_tag="VNV63_07985"
     CDS             complement(1195..>1486)
                     /locus_tag="VNV63_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62599.1"
                     /translation="LVMDPINNNSTVTFVDLHATTNELLQANLIMPEPCPFLNRKFPI
                     CSIIRPTETKGAAMGAVKALTADGLFMGQTPEFMQLLTDLATDADAAGREFD"
BASE COUNT          311 a          387 c          390 g          398 t
ORIGIN      
        1 gctagaagtc gctttcagag aggtccgtgc agctgcgttc gtagccgaag gcgagaagcc
       61 catgggggag gggagagcgg tggtaatcgc cggaccggaa gcgatcgcag catttccacg
      121 tgagaatacg aggatcggag ctcagacgcg cgttttcacg gggctaacct cccggattta
      181 ctccccacgc caggggaggg gctctattgc ggaaaatctc tcctgtgggt ttattttatt
      241 ttggctccgt gcttaaagct gatgacctga acggttcgct ttccctcatc gacgcgatag
      301 taaagcaggt aatttccatg gactagtttc aggacaccga gccgcttttt tactttggat
      361 ccacgataag gcatgaaatc aagactcatc gcaaggttga caagctcgaa gcctatttta
      421 tccgcggcat cggggttttc cgccgctata tactcaacga tacctgtgag atttttaatc
      481 gactgattgg aaaggattac cgaatacatc tccggcattt accggcgagc ttttgcaacg
      541 gtctcggcga cgagttggcg agcctcttca atggtgtacg ttctttctcc gctttccaaa
      601 gagcagatgc cttcatcgat ggctgctaag agttcagggc tctcttcctc gacatcataa
      661 gtcgcggcct catgattgag agcgttccag agctgctttt tctcttcctg tgtgagttta
      721 ggcaactctt cgagaatttc cttgatgctc atgaattctg aaaaaaagta ccccttcttt
      781 ggccctttgg ccatggaatg ttgaaaagcg gaatttcgct cttatttccc ccggtccttt
      841 accccgcccc cgtagcgccc tgaggcggtg ctttcccctt tgcctttgga aaagctcaaa
      901 atcggccgta cgtagcgctt ccgcacgccc aagcgtagtg gctgtcccct tcttttttct
      961 ggcgtgtaat cttttttgtt ttagtcagtc tggaagcctg gccgctcgaa gggtgtgctg
     1021 ggaaagagga agttatgcgc gtgcaaaagg cgtcctctgg caggctatta tcgccgttcg
     1081 ccagagctga tctcctacga ggaggttacg gactgactgt accatctaat cagggatcgc
     1141 ctgctgtcag ccttctaccg ctcttgccct cctagattgt tgaacccctt aatctcagtc
     1201 gaattcgcgt ccagccgcat ccgcgtccgt agccaaatcc gttaaaagct gcatgaactc
     1261 tggagtctgg cccatgaaaa gtccgtccgc ggtgagagct ttaactgcgc ccatcgcggc
     1321 tcccttggtc tcggtcggac gaataatcga acagatcggg aactttcggt taaggaatgg
     1381 gcatggctcc ggcataatca gattggcctg tagtagctca ttggtggtag catggagatc
     1441 tacaaacgtc accgttgagt tgttgttgat cgggtccatg accaga
//
LOCUS       DATMKH010000670         1920 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3816843, whole genome shotgun sequence.
ACCESSION   DATMKH010000670 DATMKH010000000
VERSION     DATMKH010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3816843"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="VNV63_07990"
     CDS             <1..427
                     /locus_tag="VNV63_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019645617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXC62600.1"
                     /translation="KVVVVHRRDKLRASKIMQERAMKNPKISFIWNTTVEEILGAEAG
                     HVTGAKLKNHETQTVTEEPCDGVFIAIGHQPNTKLFTGQLDMDAQGYIRTHDGTQTSV
                     PGIFAAGDVQDHIYRQAVTAAGSGCMAAIDAEKFLEANS"
     gene            442..1182
                     /locus_tag="VNV63_07995"
     CDS             442..1182
                     /locus_tag="VNV63_07995"
                     /inference="COORDINATES: protein motif:HMM:NF014016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin chelatase"
                     /protein_id="HXC62601.1"
                     /translation="MQTAILIVGHGTRETEGTCGFLELVSSVQRQVHSFVQPAFLEIA
                     PPSISEGAEYCLSTGAKHLLIIPLFLFDGQDVKKKLPQGLEEVRAAHPQVLITISPPI
                     GQDRQILEILKEHLQSVNGSCPLDETSALLVARGFSDQDAIAETEKVSIRFAQEAGLR
                     IVRHAFVEIASPSIPDGIESCLASQTKQIVVLPYLLFPGLMLKKIKNQVEAIRSRKPA
                     ETIILVSDCLGSHPKLTELLIERIRKEL"
     gene            1179..>1920
                     /locus_tag="VNV63_08000"
     CDS             1179..>1920
                     /locus_tag="VNV63_08000"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62602.1"
                     /translation="MKNRFSLKTVRYLLEYAAALPFFLIIPRLPHSWIRSLGKGLGLM
                     GYHLIGRRRRIAFANLELAYGPAMTESERRALTRQVFIHFATAALEFLWASRLSAQTL
                     GELVEMDETGLALLKNALDKGRGVLFLASHMGHWELIPVVHGYRGIGQLYGVARRQDN
                     PYLDRKINAIRCRSGTKVIYKDRAASQILRALKRNEIIGIAMDQNTSRGHVFVNFFGR
                     QAATTKAIATFALATGATILSVVCLPLPD"
BASE COUNT          421 a          543 c          529 g          427 t
ORIGIN      
        1 caaagtggtc gtcgtccacc ggcgggacaa gcttcgggcg tctaaaatca tgcaggagcg
       61 ggccatgaaa aacccgaaga tctccttcat ctggaacacg acggtcgaag aaatcctggg
      121 agcggaggcg ggccacgtca ccggagccaa gctgaaaaat catgagacgc aaaccgtcac
      181 ggaggaaccc tgcgacgggg tcttcatcgc gatcggtcat cagccaaaca ccaagctctt
      241 tacgggccag cttgatatgg atgcgcaggg atacattcgg acccacgacg gaacccagac
      301 cagcgtcccc ggcatctttg cggcggggga tgttcaagac cacatctatc gtcaggctgt
      361 caccgctgca ggaagcggct gcatggccgc tatcgacgcc gaaaaatttc tggaagccaa
      421 ctcttaagac tgtctcaaac gatgcagaca gcgatcctca tcgtcggtca cgggacccgg
      481 gagacggagg gaacctgcgg attccttgag ttggtaagtt ctgttcaaag acaggtccac
      541 tcgtttgtcc aacccgcctt cttggagatc gccccaccct ccatctcgga aggggccgag
      601 tactgcttat cgaccggcgc caaacacctg cttatcatcc cgctcttttt gtttgacggc
      661 caggatgtca agaaaaagct tcctcaggga ttggaggaag ttcgagcagc acatccgcaa
      721 gtcttgatca cgatttctcc tcctatcggt caggatcgcc aaatactcga gatcttaaaa
      781 gagcaccttc agtcggtcaa tgggtcttgc cctcttgacg agacatcggc gctgctggtc
      841 gcccgaggct tttcggatca ggacgcaata gccgagacgg aaaaggtctc gatccgtttc
      901 gctcaagagg ccgggctccg gatagtccgg catgcctttg tcgagatcgc atctccctcg
      961 attccggacg gaatcgaatc ttgtctcgct tcccaaacca agcagatcgt ggtcctccct
     1021 tatcttctct tcccggggtt gatgctgaag aagatcaaga accaggttga ggccatccga
     1081 tcccggaaac ctgcagagac cataatcctg gtctccgact gtcttggatc gcacccgaaa
     1141 cttaccgagc tgctgattga gcgaatccgg aaagaattat gaagaaccgt ttttccttaa
     1201 agacggtccg gtatcttctc gaatacgcgg cggcacttcc cttttttctg atcatcccgc
     1261 gcctgcctca cagttggatc cgttctctgg gtaaaggcct tggtctgatg ggttatcacc
     1321 tgatcggccg gcggagaagg atcgcatttg ctaatcttga gctggcctat ggcccggcga
     1381 tgacggaatc ggagcggcgg gcgctgactc ggcaggtatt catacacttt gccacggcgg
     1441 cgctcgaatt cctctgggcc tcacggttat cggcacagac cctcggagag cttgtggaga
     1501 tggacgagac cggacttgcc ttgctgaaga acgcgttaga taaaggtcgc ggtgtgttgt
     1561 tccttgcttc ccatatgggt cactgggaac tcattccggt cgttcatggt tatcgcggaa
     1621 tcggccagtt gtatggcgtg gcgcggcgtc aggataatcc ttacctggac cggaagatca
     1681 atgccatacg ttgtcgctcc ggcacaaagg tgatctacaa agaccgggcg gcgagccaaa
     1741 tccttcgggc cctcaagcgc aatgagatca tcggaatcgc gatggatcag aatacctcgc
     1801 ggggacatgt ctttgtcaat ttctttggcc gtcaggcagc aaccactaag gcgatcgcga
     1861 cctttgccct ggccaccgga gctacgattc tctctgtggt ttgcctgccg cttccggatg
//
LOCUS       DATMKH010000671         1303 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4478033, whole genome shotgun sequence.
ACCESSION   DATMKH010000671 DATMKH010000000
VERSION     DATMKH010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1303
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4478033"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="VNV63_08005"
     CDS             <1..468
                     /locus_tag="VNV63_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HXC62603.1"
                     /translation="PLLAFDPAESKWSAMHHPFTRPKSEDLPLLEAGEYGRVRAEAYD
                     VVLNGVEIGGGSIRIHEQGLQAKMFEVLGINTEQQKKLFGHLLKAFTFGAPPHGGIAL
                     GVDRLVMLITGTSNIREVIAFPKNSRGFDLMMDAPSEVPEKQLRELHIQNRKS"
     gene            complement(487..>1303)
                     /locus_tag="VNV63_08010"
     CDS             complement(487..>1303)
                     /locus_tag="VNV63_08010"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019990458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HXC62604.1"
                     /translation="RITLVALLISSALASYAEDAKPSPSSADNPSPTSAYKDDKDKVS
                     YSLGVDIGRTLQKFQLDLNEKALSQGIADVLENKPMALTDQELQETLQAFQQKMMQKQ
                     QEALSKKQEQMKGVAEKNKVDGKKFLDENAKKTGVKTTTSGLQYKVIKEGTGDKPKDS
                     DVVEANYRGTTIDGKEFDSSAKHGSASSFPVNGVIKGWSEALKLMPVGSKWELYVPSD
                     LAYGDEGAGDDIAPGSTLVFEVELLSIKKNAANPPPAPDAQPENGAEKKPAGS"
BASE COUNT          272 a          356 c          344 g          331 t
ORIGIN      
        1 cccttgctgg cttttgaccc tgcggaaagc aagtggagcg cgatgcatca cccattcacg
       61 cgacctaaaa gcgaagacct cccgcttctg gaggccgggg agtacggacg agttcgtgct
      121 gaggcctacg atgtggtgct gaacggtgtc gagatcggtg gcggcagcat ccgaatccac
      181 gagcaaggtc tgcaggctaa gatgtttgag gtgctcggta tcaacaccga acaacagaaa
      241 aaactgtttg gtcacctgtt gaaggccttt acgtttgggg ctccgccgca tggcgggatc
      301 gcccttggag tcgatcgcct ggtgatgttg atcaccggaa cgtcgaacat ccgggaggtg
      361 attgccttcc cgaagaacag ccgcggattt gatttgatga tggatgcgcc cagcgaagtt
      421 ccggagaagc aactccggga acttcatatc cagaatcgga aaagctaaag cgggacgctt
      481 cgactattac gatccagccg gcttcttctc ggccccgttt tcgggttgtg cgtccggcgc
      541 tggaggagga ttcgcggcat ttttcttaat gctcaggagt tcgacctcaa aaacaagggt
      601 tgaccccgga gcgatgtcgt caccggcgcc ttcgtctcca tacgcgagat ccgaaggaac
      661 gtagagctcc catttcgaac caactggcat caatttcagc gcttccgacc aacctttgat
      721 cacgccgttg accgggaaac tggaggcgct gccatgcttg gcggaactgt cgaattcttt
      781 cccgtcaatc gttgtccctc gataattggc ttcgaccacg tccgaatcct tcggtttatc
      841 cccagttcct tctttaatca ccttatactg aagcccgctg gtggtggttt tcacaccggt
      901 tttcttagcg ttctcatcca gaaatttttt gccgtcgacc ttatttttct cagcgacgcc
      961 cttcatttgc tcctgtttct tcgacaacgc ctcctgctgt ttctgcatca tcttctgttg
     1021 aaatgcctgg agtgtctctt gcaattcctg gtcagtcagc gccatcggct tattctccaa
     1081 cacatccgca atcccttgag agagtgcctt ttcgttcaaa tcgagttgga acttttgcag
     1141 cgtccgccca atgtcgacgc ccaaactgta actgactttg tccttatcgt ccttgtaggc
     1201 ggaggtcggg gaaggattat ctgcggaaga aggactcggt ttggcgtcct cggcatacga
     1261 tgcaagggcc gaagagatca acagagccac gagagtaatt cga
//
LOCUS       DATMKH010000672         1948 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_947632,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000672 DATMKH010000000
VERSION     DATMKH010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_947632"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(845..1480)
                     /locus_tag="VNV63_08015"
     CDS             complement(845..1480)
                     /locus_tag="VNV63_08015"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HXC62605.1"
                     /translation="MHEKEPVWHRETVDAGVERTLRDLNNVPILTSFYLAGGTGLALR
                     IGHRRSVDLDFFHGENFNEDSVLQKLQHLEGFALAAKEPGTIHASIRKTKVSLITYAY
                     PILFPFQAFLGVNVADSRDIGCMKISAIASRGTKRDFVDLYRLAQEYGLKQMLGWFTR
                     KFSQTNYSMPHILKSLCYFEEAEQDPMPHMLMSLSWEEVKQFFAREAPRLI"
     gene            complement(1518..1820)
                     /locus_tag="VNV63_08020"
     CDS             complement(1518..1820)
                     /locus_tag="VNV63_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62606.1"
                     /translation="MTESDACLILNAMIPRYLQTLFWDTNLDSFDPLAFPTYTISRIL
                     EYGNQDAIAWLKDTFSDTQIIDVIRTERRLSRRSANFWALVYGLSPDQVAALKLAS"
BASE COUNT          467 a          505 c          509 g          467 t
ORIGIN      
        1 cgccatcgga tcgctgattc atgtatggtc cacgaaccat tttcgaagct ttcagagacg
       61 tgattgccga tcgtccctgg cacatgcctc gggccatacg aaatcctctc ctctgtctgt
      121 gcgggtggta cccctatcga ctcagctcga acttacttaa aggacgcgca aagggcaaca
      181 gttgccgccc cagttggacg tcaagatctc ctgatcccaa gggcagggca tcagctactc
      241 cgttccagtg catgcgaagc tctgaggcat tgcgttcaaa agcgtcctcc tcggggctga
      301 ttcgagagaa gatgccgcaa ggattcagac gatacccgac gaagctagga ttgctaccca
      361 actaaacgaa acagttcggc ggatcggatg tttcgatttc gtggtatctc cgcgggaaca
      421 acctcagcta ggctttggaa tatcgctttc aaaggtgcat tctcagtgcc gatttgagag
      481 aagatggtcc aaggattcag agaattgcca atgatcttag gattgccgat gggctttttg
      541 atggcacgat gcgagcaggt cgatcagatt ctcggctgtg ttacctccgt gtcggcttcg
      601 cagccgaagg tgatggactt gaagatcttc catccgcccg aacaattgac aacgccatcc
      661 gtctcgctcc aggacctcgc agcagagccg actataagct tcagggataa gtctcagccg
      721 gggacccttg ctccgatcca cgttcagacg ccttgaacac tcagatgccc gccacgaagc
      781 tagcaacgga aaacagatca aaaaagagcc gatctcaatc cgagactgcc tcacttgtgt
      841 aacctcaaat taggcgcggg gcctctcttg caaagaactg cttcacctct tcccaggaga
      901 gagacatcag catgtgaggc atggggtcct gttcggcttc ttcgaaatag cataacgatt
      961 ttaagatatg gggcatgctg taatttgttt gggaaaattt cctcgtgaac catccgagca
     1021 tttgttttag accgtactct tgcgctaatc ggtaaagatc gacgaaatcg cgcttggttc
     1081 ctcggcttgc aatcgcgcta atcttcatgc agccgatatc gcgggaatcg gccacgttca
     1141 cgccgaggaa tgcttgaaat ggaaaaagaa tcgggtaggc ataagtgatc aagcttactt
     1201 tggttttccg aatacttgca tggatcgttc cgggttcctt ggctgccaga gcgaatcctt
     1261 cgaggtgttg caacttctgg agcacggagt cctcgttgaa gttttcgcca tggaagaaat
     1321 caagatcaac cgatcggcga tgaccgattc ggagtgccaa acctgttcct ccggcaaggt
     1381 aaaaggacgt caggatcgga acgttgttca gatcgcgaag cgtccgttca actcccgcat
     1441 ccaccgtctc ccgatgccat acgggttcct tttcgtgcat agttattact ccttggtgaa
     1501 ggataaacga gcgagggtta ggatgcgagc tttagcgcgg ccacttgatc gggagacagg
     1561 ccatacacca gggcccagaa atttgccgat cggcgcgaaa gcctccgttc tgtccggatt
     1621 acatcaatga tctgggtatc ggaaaaagta tccttcaacc aagcgatcgc atcttggttg
     1681 ccgtattcga ggatacgact gatggtgtag gtagggaagg cgagaggatc aaaactgtca
     1741 aggttcgtat cccaaaataa tgtctgtaag tatcttggaa tcatggcgtt aagtataagg
     1801 caggcgtccg attcagtcaa ggcagatcat tcattgggag cgcctactca caccgtatcc
     1861 ccctggcaag ctgcatggct tccgactcac ttctttctca tgaccggctc ggcgggctac
     1921 atcctcgtta cgctcgggga gcctgatc
//
LOCUS       DATMKH010000673         1155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1898676, whole genome shotgun sequence.
ACCESSION   DATMKH010000673 DATMKH010000000
VERSION     DATMKH010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1898676"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(252..800)
                     /gene="rfbC"
                     /locus_tag="VNV63_08025"
     CDS             complement(252..800)
                     /gene="rfbC"
                     /locus_tag="VNV63_08025"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009458763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HXC62607.1"
                     /translation="MRIQPIELQGVLVIEPEVFSDARGYFLETYNAKTFAEAGISELF
                     VQDNHSQSKRGVLRGLHYQNAKPQGKLIRVLQGEIFDVVVDLRPESRTYGKWSGFNLS
                     AREQKIVWIPKGFAHGFYTLSEIAAVVYKVTEFYAPQDERVILWNDPDLAICWPLQGE
                     PILSEKDKAGQSFREIRKLGRG"
     gene            complement(985..1155)
                     /locus_tag="VNV63_08030"
     CDS             complement(985..1155)
                     /locus_tag="VNV63_08030"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXC62608.1"
                     /translation="MLETLRANQWNRRKTAQALKISYRALIYKIQDAGLVSRRAATPR
                     TQEEGHLGLART"
BASE COUNT          253 a          341 c          287 g          274 t
ORIGIN      
        1 gtaggaaaag gacaggcacc ggggataatg cggaccttga accttccgta ggcgagtact
       61 ccgtggggcg tccaacttgg ctacttgagg gagtctttgg aattacagta aggacaagca
      121 agcggtttcc acctacggcc aaggaggacc gcgaagtgcc gccaagtgtc ctgccggagc
      181 cccctgtaag ccgttccttc aagtcgccta ggcacacttg gcgcgcgaaa cactccgcaa
      241 aacccgaaaa cttacccccg ccctagtttt ctgatttccc ggaacgactg tcccgctttg
      301 tctttttctg aaagaatcgg ctctccctgc agaggccagc atatcgccaa gtcgggatcg
      361 ttccacagaa ttacccgctc gtcctgcgga gcgtaaaatt ccgtaacctt gtaaacgaca
      421 gccgcaattt ccgacaacgt atagaatccg tgcgcgaatc ccttcggaat ccagactatt
      481 ttttgttccc tggcggaaag gttgaaccct gaccatttcc catacgttct cgattccggc
      541 cgaagatcca caaccacatc aaagatctca ccctgcagga cccggatcag cttcccctga
      601 ggtttcgcgt tctgatagtg gagtccccga agaacacctc tctttgactg cgaatggttg
      661 tcctgcacaa atagttcgct gatccccgct tccgcgaacg tcttcgcatt gtaggtctct
      721 agaaaatatc cccgggcatc cgaaaacacc tccggctcga taaccagcac tccctgaagc
      781 tctatcggtt gaattctcat gaatcccttc accggcccct ggagtggcag acttcattgc
      841 cggagtcgcg agtattctgt cggcccggcc cgtagacgtc cagcacccaa gcctactctg
      901 tgttgatttc ttattctttg gagaataacg cgaattcgga cgaaggagag cgcgaagggg
      961 agatttttta tttcgatgcc gaggtcaagt ccgggcgagg cctagatgcc cctcctcttg
     1021 ggtccgtggc gttgccgccc gcctggaaac caggccagca tcctggattt tgtaaatcaa
     1081 ggctctataa ctgatcttca gtgcctgcgc ggttttcctt cgattccact ggttggcgcg
     1141 aagggtttcc aggat
//
LOCUS       DATMKH010000674         1070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_313116,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000674 DATMKH010000000
VERSION     DATMKH010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313116"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VNV63_08035"
     CDS             complement(<1..272)
                     /locus_tag="VNV63_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62609.1"
                     /translation="MEILRAQIGEDRWHHFSRLLEFSCTKPEWIVLGFAAGTLAASLV
                     LAAPSEFNLPLEIIRVHGDLDERIDSFRLFQMAVERARALGAGELY"
     gene            518..>1070
                     /locus_tag="VNV63_08040"
     CDS             518..>1070
                     /locus_tag="VNV63_08040"
                     /inference="COORDINATES: protein motif:HMM:NF014919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen-debranching protein"
                     /protein_id="HXC62610.1"
                     /translation="MPLGVTYIPEEKAYNFALYSKNALEVTLLLFSEENSVTPVTEVR
                     LKPLSHKTKRVWHCRLKENDLHGAAHYAYRVDGPRNYGGSSQHAFDPEKSLLDPYAKG
                     VYFPPTFSREAAKRPGSNAGEAPLGIFRFHASNFDWKGDKSPLHFSDAIIYEVHVRGF
                     TASRSSGVAESVRGTYAGLIEKIP"
BASE COUNT          265 a          285 c          272 g          248 t
ORIGIN      
        1 caataaagtt cgccggctcc gagagccctc gccctttcga ccgccatctg aaagagtcga
       61 aaagaatcaa tcctttcatc aaggtcgccg tgcacccgga tgatctcgag tggaaggttg
      121 aactcgctgg gagcggccag aaccaacgaa gccgctagcg ttccggcggc aaaaccgagg
      181 acgatccact ccggcttcgt gcaggagaac tcgagcaacc tcgagaaatg gtgccatctg
      241 tcctcaccta tctgcgcccg gagaatctcc aaatcggatt gccacccgaa tctgtatctg
      301 caagggtcca tcggtatgcc gaacgatgtc atcaaacctt ttggatttaa atttggttta
      361 agaaaaaagg gggttttttt tgggcagagg cacggtcatt gcacagttga ccgtgtgccc
      421 gtctcacgtt aaacctgatg agttcgcaaa gctgataatg aacaagggcc agtgggaacc
      481 taaaatagtt aactggcctt ctttagaagg ttctcccatg cctcttgggg taacctatat
      541 ccccgaagag aaagcctaca attttgccct ctactccaaa aacgctctgg aggtaacgct
      601 tcttcttttt agcgaggaaa actctgtcac tccagtgacc gaagtccggc taaagccgtt
      661 gagtcacaag accaaacgcg tctggcattg tcgattgaaa gagaacgatc tccatggtgc
      721 cgcgcactac gcttatcgtg ttgatggccc caggaactat ggggggagct cacagcatgc
      781 ttttgatcca gaaaagtcgt tacttgaccc ctacgcaaaa ggagtttact tcccgccgac
      841 ctttagtcgc gaggcggcca agcgtcccgg atcgaatgcc ggagaagcgc cccttgggat
      901 ttttcgtttt cacgcatcga attttgactg gaaaggggac aaatctccgc tgcacttttc
      961 agacgccatc atttatgaag tgcatgtcag aggattcacc gctagccgct cgagcggtgt
     1021 cgcagaatcg gttcggggga cctacgccgg cttgattgag aaaatcccct
//
LOCUS       DATMKH010000675         1512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3502070, whole genome shotgun sequence.
ACCESSION   DATMKH010000675 DATMKH010000000
VERSION     DATMKH010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3502070"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..1030)
                     /locus_tag="VNV63_08045"
     CDS             complement(80..1030)
                     /locus_tag="VNV63_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXC62611.1"
                     /translation="MIIVEKLTKSFGPKLAVDNISFSVEKGEVLGFLGPNGAGKSTSM
                     RMITGFLSPSAGKVSVCGFDVVEQGLKSRSKLGYLPENAPMYSDMSVFGFLSFCAELR
                     GLYGSAKQKAIDRAVQLCFLEPVLLQSVDTLSKGYRHRTCLAQSIIHDPEVLILDEPT
                     DGLDPNQKHEVRTLIRDMGRDKAIIFSTHILEEVEAACTRAIIIDRGKIVANGSPDQL
                     KARAPLAGTIVIGVQSVAWPSIEHELAGLHFLAKSELISESASRTMFRLHPNGDLLPE
                     FLALAQRQRWPIEHLNVDEGRLDDVFRSITLPDTERASVE"
BASE COUNT          354 a          395 c          418 g          345 t
ORIGIN      
        1 aaaatcacga taaagacata cgccaccggt gaggagaaat aggcacccag ctctctccgg
       61 gcgatcgtcc agatagtttt cattcgacgg aagctctctc ggtatcgggc agggtgatcg
      121 accggaacac atcatccagt cggccttcat cgacattcag gtgttcgatt ggccatcgtt
      181 gtctctgtgc caaggccaaa aactcgggta ataaatcccc attcggatgg agccggaaca
      241 tcgttcggga ggctgattcc gagatcagct cggacttagc caaaaagtga agtccggcta
      301 gctcgtgctc gatcgatggc caggcaacgc tttggacccc aatgacaatg gttccagcca
      361 aaggggcgcg ggccttcagt tgatccggtg agccgttagc aacgattttt ccccgatcga
      421 taatgatcgc cctggtacag gcggcttcga cctcttcgag gatgtgggtc gaaaaaataa
      481 tcgctttgtc ccgccccatg tcgcggatca gggtgcggac ttcatgtttc tggtttggat
      541 cgagaccatc ggtcggttca tccaggatca aaacctccgg gtcgtgaatg atcgattggg
      601 cgaggcaggt ccgatgacga tatcccttgg aaagggtatc cacgctttgg agcaggaccg
      661 gctccagaaa gcagagctga acggctcgat cgatggcttt ctgtttcgcg ctgccgtaga
      721 ggccgcgtaa ctctgcgcag aaactgagga agccgaacac cgacatgtcg ctatacatcg
      781 gcgcgttttc tggaagatag ccgagcttgc tccggctttt caatccttgc tcgacgacat
      841 caaaaccaca gaccgaaacc ttgccggcgg acggcgacag aaagccggtg atcatgcgca
      901 tgctggtcga tttgccagcg ccatttggtc cgagaaaccc caggacctct cctttctcaa
      961 ccgagaacga tatattatcg actgcgagct tcgggccgaa gctcttcgtt aacttctcga
     1021 caatgatcat gttcgggata gggcacctgt atcagcggac tgggttaatg gactttaggg
     1081 agagcgtcta aaaaaggcgg atgccgcaat ttgtcaagcg cgtccggaga aaatagagca
     1141 ttcgcggtga ccggaaagca ttccgtgttc ggcaattgat gagtggatct tccctttatc
     1201 gcgctacgat ctgcgaaatc tgtggtttgc ctctcatctt aggatttttg gtttaaactc
     1261 tgggaaaggg ctgtaagcgg accgtcttga gctgccttgg gagttgatga ggccaaagcg
     1321 gaggcgcttt gctctacaca aaacatcgaa agcgagcaat ggaatctatc cctctcaccg
     1381 cagaacccct ctggcggcca catccggaac agatcaggaa tagcaacctt gccaagttcc
     1441 gggattggtt gcgagagaat cgctccttgg atctcagaga ccaccatgct ctctacgatt
     1501 ggtccgttgg ag
//
LOCUS       DATMKH010000676         1387 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_950397,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000676 DATMKH010000000
VERSION     DATMKH010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_950397"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VNV63_08050"
     CDS             complement(<1..207)
                     /locus_tag="VNV63_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62612.1"
                     /translation="MNTPETIRSKISEVIRAKQHALEKMESVLSDPAATPAALDEAQS
                     EVDHLTAQWNRLIHELRVVQFESPE"
     gene            412..>1387
                     /locus_tag="VNV63_08055"
     CDS             412..>1387
                     /locus_tag="VNV63_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62613.1"
                     /translation="MATHYSPKQFIRQVPNTLLKAFFDRRGELADIGWEKLGEMDADP
                     IFEAWQALPEGNRNDVERWFRTIGDLATAEGLQTLIEEGHFHGVELTVPLGQLKGLHE
                     KAFWVYLNQERIFTSAGRLNRADQLTGRYLKRRLLPVKKPDISHPTRKLLADAISQFY
                     REEQGRGEHCNIEVYLRREKIHYFFAYPADYADTVIIYTDAGSLKRQLQKAAFEVIFA
                     YNEGSGALDLFVQGDKKVRHEMEGIFCRLILKEELPAEELDANPFELNGLRSRDFDFP
                     TDPEDGIQEVRVKALRLSIVGPGFGRITFESDARTKRGDIYELMDKALN"
BASE COUNT          331 a          347 c          398 g          311 t
ORIGIN      
        1 ctccggagac tcgaactgta ccaccctcag ttcgtggatc agccgattcc actgagcagt
       61 caagtggtca acttcactct gggcctcatc caacgcagcc ggggtagcgg cagggtcact
      121 gagaacggat tccatcttct caagtgcgtg ctgttttgcc ctgatgacct ctgagatctt
      181 cgaccggatc gtttctggag tgttcacaac agaatcgtat cagatccatg atgtcccacg
      241 caataccatt tatttctcat tgccaccacg gggtaccaga tgatagaatc caggcatggg
      301 ccgtgcgatt gggttcgatt tcttcggtga acaaagaact ggtaataggc aacacaaacc
      361 gaggatttcg cctggaaagc gtgatgccaa actactgagg agataagcct tatggctacg
      421 cactacagtc cgaagcagtt cattcgtcag gtacctaata ccttattgaa ggcgtttttc
      481 gaccgtcggg gggagctcgc ggacattgga tgggagaaac tcggggaaat ggacgccgat
      541 cctatcttcg aggcctggca ggcactgcca gagggaaatc gcaacgatgt tgagaggtgg
      601 ttccggacaa tcggggatct tgcgaccgca gaggggctgc aaactctgat cgaagagggg
      661 catttccacg gcgtcgagct gacagtcccg ctcggacagc taaaagggct ccatgaaaaa
      721 gccttctggg tttatctgaa ccaggaacgg attttcacat ctgcggggcg gctgaatcgc
      781 gctgaccagt tgaccggacg ctatttgaag cggagacttc tccccgtgaa gaagcccgat
      841 atcagccacc ctacgagaaa gcttctggca gacgcgatct ctcagtttta tcgagaggaa
      901 caggggcgtg gcgagcactg taacattgag gtttacctcc ggcgcgagaa gatccattac
      961 ttttttgcgt atcccgccga ctacgcagat acggtcatca tctataccga tgctggttct
     1021 ttgaagcggc aactgcagaa ggcagcgttc gaggtgatct tcgcgtataa cgaaggatcc
     1081 ggcgcactgg atttgttcgt gcagggcgac aagaaagtcc ggcatgagat ggaagggatt
     1141 ttctgtcggc tgattctcaa agaggagttg cccgctgaag aactcgatgc gaatccgttc
     1201 gagctgaacg ggttgcgaag ccgtgatttc gactttccca ccgatcccga ggatggaatc
     1261 caggaagtac gggtcaaagc actccggctg tcgatagtcg gacccggctt tggtcgtatc
     1321 acttttgaga gtgatgcacg gacgaagcgg ggggacatct acgagttgat ggacaaggcc
     1381 ctcaatc
//
LOCUS       DATMKH010000677         1843 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2877766, whole genome shotgun sequence.
ACCESSION   DATMKH010000677 DATMKH010000000
VERSION     DATMKH010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2877766"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            381..>1843
                     /locus_tag="VNV63_08060"
     CDS             381..>1843
                     /locus_tag="VNV63_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017253927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HXC62614.1"
                     /translation="MNSNATLPSIAHPNRERALKRARLTAGFPNIVNGQRIDSSKRIQ
                     ISDPVTGEELASVTDTQKAGLDQAVKAAKAAFPLWSSKTWNERRDLLRDAMEELKTHT
                     DELCTILTAENGRPYSIAQFEIAWVFETYAPALFQMELPDSTWNETGVGQITKRYVPL
                     GVVAAISPWNLPFLLSFTKVLPSLLAGNTVVLKPAVFTPLTVLRTADYIREILPPGVL
                     NVISGGDDLGPWMTSHPDFQKVSFTGSSETGRKVFASAAPTLKHLTLELGGNDPGIVL
                     PDADPQAIAQYLFQSMFLLSGQGCVTLKRLFVHESIYQALTEALISCARHQKLGDGFD
                     PETTLGPIQNRPQFARMQTTWEEIQKAKAPILYQGEVPSEGAGLFFPVTLLDNPPAEA
                     AYVIRENFGPLRAVFKYKDLDEALRLANGTPYGLGASVWGTDPESLDAVARRLDAGMV
                     WINQHLNLHPNIPFSGHKNSGIGIEFGEDGLKEFCNV"
BASE COUNT          461 a          531 c          456 g          395 t
ORIGIN      
        1 aatccctcag tctttgcaac agagcgctgc ccaacccaca acctctaacg cttgcggtgc
       61 ggattcacac agggagttcc tctggaccaa atcgcaccac cgtcacagaa atttccagat
      121 gaattgaaac gttacggata aaagcgaaca tggcgtaccg ccagatggat tctgatgtct
      181 ttagaacgat tggccagccc ggtaatcgcg acgagatgtg agcaagattc gcagaatgct
      241 gacgaagttc taggttgaat gaggaggaac accaaaagag aaaaccgatt cgttcaacga
      301 gacaatacaa ccccaactgg aagccacttt aacccacaac ccgagatctc agaccgagtc
      361 ttggcgaaag gaccttttgt atgaactcaa atgcaactct cccatcgata gcccatccca
      421 atcgcgagcg agcgctaaaa agggctcgcc tgaccgcagg gtttccgaac atagtgaacg
      481 gccaacgaat cgactcttca aaacggatac agatctccga tcctgtgacc ggagaagaac
      541 tcgcaagcgt gaccgacacc cagaaagctg gtctcgacca ggcagtcaag gctgcaaaag
      601 cagcttttcc cctttggagc agcaagacct ggaacgagcg aagggatctt cttcgagacg
      661 ccatggaaga gctcaaaaca catactgatg aactctgcac gatcctgaca gcggaaaacg
      721 gccggcctta tagcatcgcg caattcgaga tcgcgtgggt tttcgagact tatgctccgg
      781 cgttattcca gatggaactc ccagattcga catggaacga aactggtgtc ggacaaatca
      841 caaagcgtta tgttccactc ggtgttgtcg ccgcgatcag tccgtggaat cttccgttct
      901 tgctgtcatt caccaaagtg ctcccatctc tgctggccgg caacaccgtt gtcctcaagc
      961 ctgcggtctt tactcctctt acggtacttc gaaccgccga ctacattcgt gagatccttc
     1021 cgcccggcgt tttgaatgtg atcagcggag gggatgacct tgggccctgg atgacgtccc
     1081 acccggactt ccaaaaggtc tccttcacag ggtcttcgga aaccggacgc aaggtcttcg
     1141 catccgccgc tccaaccctg aagcacctga cccttgagct aggcgggaac gatcctggta
     1201 ttgtccttcc ggacgccgat cctcaggcaa tcgcccagta tctgtttcaa agcatgttcc
     1261 tactcagtgg ccaggggtgt gtcaccctca aacgcctctt cgtgcacgag agcatctatc
     1321 aagctcttac ggaagcactc atatcgtgtg ctcgacatca gaaactcggc gatgggtttg
     1381 atcccgagac caccctgggc cctatccaga accgccccca gttcgctcgc atgcaaacaa
     1441 catgggaaga gattcaaaag gcgaaggctc ccatcctcta tcaaggggaa gttccgtcgg
     1501 agggtgccgg cctgtttttt cccgtcaccc tcctggacaa tccgcctgcg gaagcggctt
     1561 acgtaatccg agaaaacttt ggaccattgc gggccgtttt caagtataag gacctcgacg
     1621 aagccctccg gctggccaac gggactccat atggccttgg agcctccgtt tggggtacag
     1681 atcctgagtc actggacgcc gttgcgcgtc ggcttgatgc cggaatggtt tggatcaatc
     1741 aacacctcaa tctccatccc aacattccat tcagcggtca taagaattca gggattggca
     1801 ttgaattcgg cgaagatggg ctcaaagaat tctgcaatgt gca
//
LOCUS       DATMKH010000678         2045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1900674, whole genome shotgun sequence.
ACCESSION   DATMKH010000678 DATMKH010000000
VERSION     DATMKH010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1900674"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="VNV63_08065"
     CDS             complement(<1..575)
                     /locus_tag="VNV63_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62615.1"
                     /translation="MPPEINRRRAVLVLGKIDEILSWEKAKEQEEDTRFVELGECLCE
                     VRSNQYWRLEKLKSFDEFLERRFPGSRRKAYYLMAIHEHLSRIAKPELRLMGWTKARE
                     LVKVARRDGQKFDCAPWVHKASLMPREDFKQEVERHLTGKETEPWEIIYFKLYKSQLP
                     VIEQALETAALMLGTDKSRGYCLEMICADFLA"
     gene            complement(614..1411)
                     /locus_tag="VNV63_08070"
     CDS             complement(614..1411)
                     /locus_tag="VNV63_08070"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HXC62616.1"
                     /translation="MGREAAMVYSYTQISQYLRCPRSYRYRYLDGWREKETRAAMVFG
                     RCFEKALGAYFCQEDSAAMLFKEWEAYRDASFEYRKGESWDRLFHQGVHLLETFARDN
                     RIRIHSPKKNLQIKIHRTLPGGSEFVAYLDALGEVDDQHSVIDWKTTTSRYSTEPGGL
                     LSLDPQLICYSWITGISDVAFVVFVEKTCPRDPIPEGVDLRRAAPRIRPTRRDHHQSD
                     RSGSLCFAQRDPLSSERLRELSASGPLSRQQRNGQHQSHPKAGSERP"
     gene            complement(1383..1826)
                     /locus_tag="VNV63_08075"
     CDS             complement(1383..1826)
                     /locus_tag="VNV63_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62617.1"
                     /translation="MKRHIPGLFLNQRGVENKLEGLFLVRVDRARYRWHPQRPFVELR
                     FVILEPKPFETRVFSGRLYCTERALWKLNWFLRDFGYDAQLLKEDQVDEKALCHLRGV
                     IRTSYIALNGRSYQNLDAFAPVADWEALECTSLAQSDGQGGSDGL"
BASE COUNT          460 a          575 c          526 g          484 t
ORIGIN      
        1 gctagaaaat cagcacagat catctccagg cagtaacccc tagacttatc agttcccagc
       61 atgagcgctg ccgtttccag ggcttgctca atgaccggca actgactctt gtaaagcttg
      121 aaatagatga tttcccaagg ctcggtctcc ttaccggtga ggtggcgctc gacctcttgc
      181 ttgaaatcct ctctcggcat caagctggcc ttgtgcaccc agggtgcaca atcgaacttt
      241 tgcccatccc tccgggccac cttcaccaac tccctggctt tggtccatcc catcagcctt
      301 agttccggtt tggcgattcg cgacagatgc tcgtggatcg ccatcaggta ataggccttt
      361 cggcgcgacc cagggaaacg cctttcaagg aattcatcaa atgactttaa cttctcgagc
      421 ctccagtatt gattcgatcg aacctcgcac agacattctc caagctcgac aaaccgcgtg
      481 tcttcctcct gctctttcgc cttctcccag gacagaatct cgtcgatctt gcccaatacc
      541 aacacagctc ggcggcggtt gatttcagga ggcacagtcg tccgcctgtt aatccacaag
      601 ctcgtcaagc caatcaaggt cgctcgctcc cggctttcgg atgagattga tgttgaccat
      661 ttctttgttg tcgagacaaa ggcccagatg cggacaactc acgcaaccgt tctgaggaaa
      721 gcggatcccg ctgtgcgaaa caaagtgacc cgcttcgatc tgattgatgg tggtctcgac
      781 gagtcggccg aattcttggc gctgctcttc ggagatcgac gccttcaggt attggatctc
      841 gggggcatgt tttctcaaca aacaccacaa aggccacatc agagatcccg gtgatccagg
      901 aataacagat gagttgcgga tcgagcgaca gcaatccccc gggctccgtc gaatagcggc
      961 tggtggtggt cttccagtcg atcacggagt gctggtcatc cacctcccct agggcatcaa
     1021 gataggccac gaattcgctg cctcccggca gtgtccgatg tatcttgatc tggagattct
     1081 tttttggact gtgaatccga atgcgattat cccgcgcaaa tgtttcgagt aagtggaccc
     1141 cctggtgaaa gagccggtcc caggactcgc ctttgcggta ttcgaaagaa gcatcgcggt
     1201 aggcttccca ttctttgaaa agcattgcgg cgctatcctc ttggcaaaag taggcaccta
     1261 gagccttttc aaagcagcgg ccgaacacca ttgccgctcg tgtctctttt tcgcgccacc
     1321 catccaggta acggtagcga tagctgcggg ggcaacgcag gtattggctg atctgcgtgt
     1381 agctatagac catcgctgcc tccctgccca tcgctctgag ccagtgatgt gcattcgagt
     1441 gcttcccagt ctgccacggg agcgaaggca tcgaggtttt ggtaagagcg tccgtttaga
     1501 gctatataag aggtccggat gaccccgcga agatggcaaa gcgccttttc atcgacctgg
     1561 tcctccttga ggagctgcgc gtcatagcca aaatctcgaa gaaaccaatt gagtttccag
     1621 agagcccttt cggtgcagta caaccgtccg gagaagaccc gggtttcaaa aggcttgggc
     1681 tccaaaatta caaatcggag ctctacgaat ggcctttgtg gatgccatcg gtagcgagcc
     1741 cgatccacgc gaacaagaaa taagccttca agcttgtttt cgactccacg ctggtttaaa
     1801 aaaagaccag ggatatgtcg tttcattgcg ctacctccac gggttgagca taggaattca
     1861 gtttgcagac caagccgtcc ctgttttcct ttgcggcggt cgccagatga gagataaagg
     1921 actcaaccaa atcgcgactg gcgtctttga ggatcggagt gccgcaaaag tcagcggcgt
     1981 acgctttgac cagtacggga tcgaggttgt actggcggat gagcacacac agtcggtctc
     2041 gcagt
//
LOCUS       DATMKH010000679         2563 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2218511, whole genome shotgun sequence.
ACCESSION   DATMKH010000679 DATMKH010000000
VERSION     DATMKH010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2218511"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="VNV63_08080"
     CDS             complement(<1..471)
                     /locus_tag="VNV63_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005181864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HXC62618.1"
                     /translation="MKLSGNRLVFNSNLQALGLSALGVVFGDIGTSPLYTLKTVLALT
                     GANPDPAVTLGTLSLLIWTLIVVTSVKYVSFVMSIDNDGEGGILALMSLLGVKLRPRP
                     VIIALGLLGAALIYGDGALTPAISVLSAVEGLNIVNPSFAPYVLPASVLILIAFF"
     gene            complement(468..1934)
                     /locus_tag="VNV63_08085"
     CDS             complement(468..1934)
                     /locus_tag="VNV63_08085"
                     /inference="COORDINATES: protein motif:HMM:NF039904.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HXC62619.1"
                     /translation="MRMTKKAMRDEPQASRNVPPLDSHAQYEGTSLLADPVHEYITFT
                     VPATRDPDEVTEKDLIDSPWVQRLRYVYQLQSARWVYPTAEHSRFQHSLGAMHVAGRF
                     AQHLYPSLKETLGDIPSPAYIEELLRIAALLHDVGHGPFCHFFDDHFLDEYKATHELI
                     GQAIIKEKLGSLIKKIRRAPSGPFRSDERLDPDQIAFLILKDPYKDSSRYPPWLSLLQ
                     PLLGGIYTADNLDYVLRDSFMCGVAIGPVDLSRLIHYTFFTTKGLTLHKAGLSALQMF
                     LNARSYLYSNVYYHRTTRAIDRHLRDIFRPTIKHVFPYHPLQQLDRYVDLTDWSLIEA
                     VRGWKVSRSREKRRLYGEWQKILQRQPTWKMAYETVLPTSGMEQGRIFMDEKAIEDGI
                     RKALPLHLKKLEFRVDMANQDPRPLNPLSMGEFQIHVYDPSTQTVSKEGLTELFKFIP
                     AKIAQCRIFARSHEHDRFLAEIARKVLGTERSSSKTNL"
     gene            2229..2429
                     /locus_tag="VNV63_08090"
     CDS             2229..2429
                     /locus_tag="VNV63_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HXC62620.1"
                     /translation="MARGKVKWFNATKGYGFISQENGGDVFVHFSAIQGEGFKTLEEG
                     QEVEFEVTDGPKGPQAANVTKV"
BASE COUNT          658 a          625 c          680 g          600 t
ORIGIN      
        1 aaaaaaagca atgagaatca ggacggaagc gggcaggaca tagggcgcga aagaaggatt
       61 tacgatgttc aacccttcga ccgcagacaa cacggagatc gcgggtgtaa gcgccccgtc
      121 gccatatatc agcgcggcac ccagaagccc aagcgcgatg ataacgggtc ggggcctaag
      181 tttgacaccg agcaacgaca tgagagcgag gatgcctccc tcaccatcgt tgtcgatgct
      241 catgacaaaa gaaacatatt tgacacttgt cacaacaatg agggtccaga tgaggagcga
      301 aagcgttcca agggtaacgg ctggatcagg gttcgcgccg gtgagggcaa gaactgtttt
      361 tagtgtgtag agagggcttg ttccgatatc gccgaaaacg acacccaacg ccgacaggcc
      421 caaggcttgc aggttcgagt tgaaaacaag gcggtttccg gataatttca taaattggtc
      481 ttgctgctgg atcgctccgt ccccaggacc tttcgtgcga tctcggccag aaagcgatca
      541 tgctcatggc tgcgggcaaa aattcggcac tgcgcgatct tggccgggat gaacttgaac
      601 aattccgtga ggccctcttt ggaaacggtt tgggtggatg gatcgtatac atggatctgg
      661 aactcaccca tactgagcgg attgagggga cgcgggtcct ggttcgccat gtccactcga
      721 aactccagtt ttttaaggtg gagcggcagc gccttccgaa tcccatcctc aatcgctttt
      781 tcatccatga agatccgacc ttgctccatc ccactggtgg gcagcaccgt ctcatacgcc
      841 atcttccacg tcggttgtcg ttgaagaatc ttttgccact ctccataaag tcttcgcttc
      901 tctctcgagc gactcacctt ccagccgcga accgcttcga taagagacca atcagtcagg
      961 tccacgtaac gatccagttg ctgtagagga tgatagggaa aaacatgctt gatggtggga
     1021 cgaaagatat cacggagatg gcggtcaatc gcgcgtgtgg ttcgatggta gtagacattc
     1081 gagtagagat aagaccgtgc atttaagaac atctgaaggg cagagagccc cgctttatgc
     1141 agtgtcagac ccttcgtggt aaaaaaggtg taatggatca acctcgaaag atctaccggt
     1201 ccgatcgcca caccgcacat aaaagagtcc cgcaacacgt aatcgagatt atccgccgtg
     1261 tagatcccgc cgagcaaggg ctgaagcaaa gaaagccatg gaggataacg ggaactgtcc
     1321 ttgtacggat ccttaagaat caaaaaagcg atctgatcgg gatccagtcg ttcatcagac
     1381 cgaaatggtc ccgaaggagc acgtcggatt tttttaataa ggctgcccag cttttccttg
     1441 atgatggcct gaccgatgag ctcatgggtc gccttgtatt catcgagaaa atggtcgtca
     1501 aagaagtgac agaatggccc atgacccaca tcatgcaaaa gcgccgcgat ccggagcagc
     1561 tcttcgatat aggctggtga ggggatatct cctaaggtct cttttaagga tggatagagg
     1621 tgctgtgcaa agcgtccagc gacatgcatc gcccccaggg aatgttgaaa gcggctatgc
     1681 tcggcagtgg ggtacaccca tcgggcactc tgtaactggt agacataccg aagccgctgc
     1741 acccagggtg agtcaatgag atctttctcg gttacctcat caggatctcg tgtggcggga
     1801 accgtaaagg tgatatactc atgaacgggg tcagcaagca aggaggtccc ttcgtactgg
     1861 gcatggctgt ccaacggagg cacatttcga gaggcctgcg gctcatccct catcgctttc
     1921 ttcgtcatcc tcatcgtcgt catcctcatc gccgttaagg ttgtcatctt ccgtgtcttc
     1981 atcgtcgtct tcgaaatcat ccccgttcat ctttccctcc tttatttgag tcaggatatt
     2041 ttatctgcta accactgtcg tttatttata acctgagcgg tcataagctg tcaaggactc
     2101 ggtcaccgaa tcggactcct taaatttttc ttgacaatct ccggaatgct atggtacaca
     2161 gacaagggtt tgtcatagtt taaaaatcct gtttttgttt gcaatataga aaggaggaaa
     2221 agtcaagaat ggcacgagga aaagtcaaat ggttcaatgc caccaaggga tacggcttta
     2281 tttcccagga aaatggcggt gatgtctttg ttcacttctc ggcgattcaa ggagagggtt
     2341 ttaaaacatt agaggaaggc caggaagtcg aatttgaggt cactgacggc ccgaagggcc
     2401 ctcaggctgc taacgttacc aaagtctaat caggatacga ggccagtttg agaaaaatga
     2461 aagccccgga ggttttattt ccggggcttc tttaatgagt gcaaaggcgt cccagactgg
     2521 ttcataagcc gggttctgta tccgtcacaa catgcggacg gat
//
LOCUS       DATMKH010000680         2938 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3185913, whole genome shotgun sequence.
ACCESSION   DATMKH010000680 DATMKH010000000
VERSION     DATMKH010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3185913"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="VNV63_08095"
     CDS             <1..484
                     /locus_tag="VNV63_08095"
                     /inference="COORDINATES: protein motif:HMM:TIGR01905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HXC62621.1"
                     /translation="NPPLWDKKTTVKSFGLAPLTPVETPQPSYPFGPSSSCLACHDDA
                     LAAGIHDPKLNHPVASAYPRQPDGHFVARRNTPDLARYWSIPDRTSSGVSLPTGPHSE
                     YLSLPVGSDSKDPAITSRMVRTTAGFIHCDSCHNPHNDSTAPFLRASSKTLCLICHDR
                     "
     gene            513..>2938
                     /locus_tag="VNV63_08100"
     CDS             513..>2938
                     /locus_tag="VNV63_08100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HXC62622.1"
                     /translation="MPPGQAGRPLVAVAAFLVIGLVLGERFSYFPLLVFGSLVMGLLL
                     WRWAQASSVPLGLILLPLLFGLFYYQLAVRYRAASDLSPYLDQNLMTFEGQLADPVRH
                     YPDRLVGILAVDKVLLQGEERPARGRVRLSLDWPESGFLPGDLLRIEAKLRSPLGFKN
                     PGGFDYAEYLRRDGIQAVSTISHPERVLNLGKAPGVMMRRIAIWREKIRVAIANSLAA
                     HEAAILQAMIIGESGYLTNEIRDAFMASGTTHILSISGSHLGLVAFVVFLLVRRSLRL
                     LPAPIFLRLTIWLTPSQIAACVTFFPVVFYALIAGGQSATVRSLIMILLYLLAVILQR
                     EDDLLNALAFAAILTLLWEPQAIMDISFQLSYGSVLAMGLFLRWWEEHKPEEVEMTSR
                     ARLIRSSVLMLLLTLTTTVWTAPLVAYHFHQVAWVGLFSNLIIIPMAGFLIVPLGLIS
                     SVLVLIFNLDYLPLAWFNQHGLAIFDGLVQKFSRLPAAEFHLPAPSLVFIIVFYLLLF
                     FLFWTKKIQTPRFLGGSLALVFGLLMVWGLLPRLPDRTLRISYLDVGQGDSALIEFPE
                     NKAMVIDGGGAFGDFDLGRIVVAPYLWDRRIHRIDYLVATHPQQDHLGGLSYLLKKFD
                     IGNVWTNGTRRDTLLYQTFSQALDQKGLKEREMFRNKEALEIGGCRVWILNPFQTADS
                     EGGGYRSKADNNRSVVLRLEKGDDSFLFTGDIEASAERMLATMEGEVRSRVLKVPHHG
                     SRGALDDEFLGRVRPEIAIISVGAHNSYGHPAPEVLDAYQGLGAKLYRTDQEGAILIS
                     SDGEKLSIQT"
BASE COUNT          567 a          779 c          797 g          795 t
ORIGIN      
        1 taatccgcct ctttgggata agaaaactac ggtcaagtca tttgggttag caccgctcac
       61 gcccgttgag acgccgcagc cgtcctatcc ttttgggcct tcctcatcct gtttggcatg
      121 ccacgatgat gccttggctg cgggaattca tgatcccaag ttgaaccatc ccgttgcatc
      181 ggcttatcca cgacaaccgg acggccactt cgtggcaagg cggaatacgc ctgatctcgc
      241 tcgttactgg tccattccgg atcggacctc atcaggggtc agtctgccca ccggccccca
      301 ttcagaatat ctctctttgc ctgtcggctc agattctaaa gatccggcca ttacatccag
      361 gatggttcgg actacggcgg gttttatcca ttgtgatagt tgccacaacc cccacaatga
      421 cagcaccgca ccgtttttaa gggcctcttc aaagacgctc tgcctcatct gtcatgaccg
      481 atgatgagcc aaatcgcagt gtagttttca acttgcctcc cggacaagca ggaagaccgc
      541 ttgtcgcggt agccgcattt ttagttatcg gtcttgttct gggtgaaagg ttttcttatt
      601 tccctctctt ggtcttcggt tcattggtca tgggcctgct cttgtggcga tgggcccagg
      661 cttcttccgt gccgttgggt ttaatcctcc tccctcttct cttcggtctc ttttattacc
      721 agcttgccgt acggtatcgt gcagcatccg acctatcccc ttatctggat cagaacctca
      781 tgacctttga gggtcaactt gcagatccag tgcgacacta tccggatcgt ctggtcggca
      841 tcctggcggt tgataaggtg ctgcttcaag gagaagagcg cccggcccgg ggtcgggtgc
      901 ggctttcttt agattggccg gaatcaggtt ttctcccggg ggatcttctc cggatcgaag
      961 ccaaattacg atctccgctg gggttcaaaa atccgggcgg atttgactat gccgaatatc
     1021 tccgtcgcga cggcatccag gccgtgtcga cgatctcaca tccggagagg gtgttgaatc
     1081 tcggaaaagc gcccggtgtg atgatgagga ggatcgcgat ctggcgggag aagatccggg
     1141 ttgcgatcgc aaattcactc gcggcgcatg aagcggccat tctgcaggcg atgatcattg
     1201 gggaatcggg ttacctcacg aatgagatcc gggatgcttt tatggcttct ggaaccacac
     1261 atatcctgtc tatctccgga tcccacctgg gcctcgtggc ctttgtagta ttcctgctgg
     1321 ttcgacggag tttacgtctt ttacccgcac ccatttttct tcggctgacg atctggttaa
     1381 ccccttctca gatcgccgcc tgcgtgactt tcttcccggt ggttttttac gctctaatcg
     1441 cgggtggtca atctgccacg gtacgatcct taatcatgat cctcctctac ctcttagcgg
     1501 tgatccttca gagggaggat gatctcctga atgccttggc ctttgcggct atcctgacgc
     1561 tcctctggga accgcaggcg atcatggaca tctcgtttca gctctcttac ggatccgtgc
     1621 ttgccatggg cttgtttctg agatggtggg aggaacataa acccgaagaa gtcgagatga
     1681 catcccgggc ccgcctgatc cgatcctcag tcctgatgct ccttcttacc ttgacgacga
     1741 cggtctggac cgcaccattg gtagcctacc atttccatca agtggcctgg gtaggtttgt
     1801 tttcgaatct tatcatcatt ccaatggcgg gttttctgat cgtcccgttg ggactaatca
     1861 gttccgtcct cgtgctcatc ttcaatctag actatttgcc gttggcctgg ttcaaccagc
     1921 atggtctcgc gatctttgat gggctagtcc aaaaattttc caggctgccg gctgccgagt
     1981 tccacctgcc ggcgccatcg ctcgttttta tcatcgtatt ttatctgctg ctgttcttct
     2041 tgttttggac caagaagatt cagactcctc ggtttttggg tggctcactg gcgctggtgt
     2101 ttggactcct gatggtttgg ggacttctgc cgcgtttgcc ggatcgcaca ttgcgtatca
     2161 gttatctgga cgtcggccaa ggagactccg ccctgatcga attcccggaa aataaggcca
     2221 tggtcattga tggcggtggg gcctttggcg attttgatct tggtcggatc gtggtggcgc
     2281 cgtatctgtg ggatcggcgc atccaccgaa tagattacct ggtggcgact catccacagc
     2341 aggatcacct tggaggtctg agctatttgt tgaagaaatt cgatatcgga aatgtctgga
     2401 ccaatgggac ccggcgagac actttactct atcagacatt ttcccaagct ctcgatcaga
     2461 agggtttaaa agaaagagag atgttccgga acaaagaagc cctggagatc ggtggctgtc
     2521 gggtctggat cctcaatccg tttcagacgg cagacagcga gggaggcggc tatcgttcca
     2581 aagcggataa taaccggtcc gttgtgttgc gacttgaaaa gggcgatgat tcctttttat
     2641 ttacggggga cattgaggcc agtgcagagc gaatgctcgc gacaatggaa ggggaagtaa
     2701 ggagcagggt tctcaaggtt ccgcatcatg gaagccgggg cgccctggac gatgaatttt
     2761 taggccgagt ccgtccggag atcgcgatta tctcggtcgg ggcccataac tcgtatggac
     2821 atcccgctcc ggaggtgctg gatgcctatc aggggttggg cgcgaagctt tatcgaaccg
     2881 atcaggaggg cgctattttg ataagttcgg atggggagaa actttcgatc cagaccga
//
LOCUS       DATMKH010000681         1000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2542186, whole genome shotgun sequence.
ACCESSION   DATMKH010000681 DATMKH010000000
VERSION     DATMKH010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2542186"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..316
                     /locus_tag="VNV63_08105"
     CDS             65..316
                     /locus_tag="VNV63_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62623.1"
                     /translation="MDSLFRVTTLTVPTADSAGTSAPGREEAVATGAQCLSALGMIAV
                     MLMVLPMQSVLEVIPLNLEEMAKEITRQLDEAVSELSKS"
     gene            362..934
                     /locus_tag="VNV63_08110"
     CDS             362..934
                     /locus_tag="VNV63_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62624.1"
                     /translation="MPTESYKQIIDPETDPDRIVQWWMVESVDLIRIFYQQHLGPSFT
                     EIPAIEIPRPEYGELFDGEDAILADVFSCSPQLLEDIALIYTRSSQDSVKVAMNLESE
                     ACGERIIEFLAGHTTYTLHNKFRRGLETLAERVQVHHYTWQRDRGNLAPFGLHRILIP
                     KASRERMDQIRKHLEEKGQEDTNSWADDEL"
BASE COUNT          254 a          268 c          253 g          225 t
ORIGIN      
        1 caattcggac aagcgtcaaa ttcacccctc acctaaagga gagggttccc ttgcctgaaa
       61 ctctatggat tctctatttc gcgtgacaac actgacggtc ccgaccgcgg acagtgcggg
      121 cacctcagct cccggtcggg aggaggctgt agcgaccggg gctcaatgtc tttctgccct
      181 gggcatgatc gccgtcatgc tgatggtttt gccgatgcaa tcggttctgg aagtcatacc
      241 tttaaacctc gaagaaatgg ccaaagagat taccaggcaa ctcgatgaag ctgtgagtga
      301 attgtcgaaa tcataaaact gacctgttct gtttaaaaac gttttaacat actgacctag
      361 tatgcctact gaatcctaca aacagatcat tgatccggaa acggatcccg atcgaatcgt
      421 ccaatggtgg atggtggaat cggttgatct gatccgaatt ttttaccaac aacaccttgg
      481 cccgagtttc accgagatcc cggcaattga aattccccga ccggagtacg gcgaactctt
      541 cgatggcgag gatgcgattc tggccgacgt ttttagctgt tcgccgcagc tcctggagga
      601 tattgccctt atctacacga gatcctccca ggactcggtc aaggtcgcaa tgaacctgga
      661 gagtgaagcc tgcggcgaac ggatcatcga atttctggcc ggccacacca cctatacctt
      721 gcataacaaa ttcaggcgcg gtttggaaac tctggccgag cgcgtccagg tgcaccacta
      781 cacctggcaa cgggaccgtg gtaacttagc tcccttcggc ctgcacagga tcttaatacc
      841 gaaagcttcc agagagcgaa tggaccagat acgtaagcat ctcgaggaaa agggtcagga
      901 ggacactaac tcttgggcag acgacgaact gtgagcccgt ctcttgagcg tcgtcttaat
      961 caccacctcc ggcgcgagag gaaggttaaa aaacatcatc
//
LOCUS       DATMKH010000682         1132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1598055, whole genome shotgun sequence.
ACCESSION   DATMKH010000682 DATMKH010000000
VERSION     DATMKH010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1598055"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VNV63_08115"
     CDS             <1..211
                     /locus_tag="VNV63_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62625.1"
                     /translation="LNQDEHLINNAGDAFKKGDIAAVAGAQLNLGPVMAGARYVVGLT
                     NLNDVTNQSTWRNEGWQLYAGFRIF"
     gene            284..>1132
                     /locus_tag="VNV63_08120"
     CDS             284..>1132
                     /locus_tag="VNV63_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HXC62626.1"
                     /translation="MKENLFLRAEDDLDFMPIIPLNESDNEGLNDMEIPKELPVLPLR
                     NTVLFPGVVLPITVGRDKSIKAVNDAYKSDKLIGVLAQKDSSIEDPGVTDLEDVGTVA
                     RIIKLIKMPDGGTTVILQGRRRFKVGAVTAEDPYFKAQVVLLQEEEAPKEQDFEAYVS
                     NIKDLASQIIQLSPNIPSEASIILKNIENPSFLIHFISSNLNTELHEKQQLLEIHHIK
                     SRADLLMQLLQRELQFAELKNKLTNRTKTELDKQQREYFLQQQLKSIKDELGGDSNER
                     EVKEMQK"
BASE COUNT          298 a          262 c          325 g          247 t
ORIGIN      
        1 gctcaatcag gacgagcatc tgatcaataa cgccggggat gccttcaaga aaggcgatat
       61 agcagcggtt gccggtgcgc agctgaatct cggtccggtg atggccgggg cgcgatatgt
      121 ggtcggactg accaatctca atgatgttac gaatcagtcg acgtggagaa atgaagggtg
      181 gcagctgtat gcgggctttc gcattttcta gccggccttt tttatacctt tcaactaaaa
      241 aagagtagga attttccctt actcgtggta ttttgtccct gaaatgaaag agaatttatt
      301 cctgcgtgcc gaggatgatc tggatttcat gccgatcata ccgttgaatg aatcagataa
      361 cgagggtttg aacgatatgg agatccctaa ggaactgccg gtccttccgc tgcggaatac
      421 ggtattgttt cccggggtag ttcttcctat tacagtaggg cgggataaga gcatcaaggc
      481 ggtgaatgac gcttataaat cggataagct gatcggggtg ctggctcaga aagacagcag
      541 tatcgaggac ccgggcgtaa cggatctgga ggatgtcggc acggtggcgc ggatcatcaa
      601 gctgatcaaa atgcctgacg gcgggacgac ggtcatcctc caggggagac gccggttcaa
      661 ggtcggcgcg gtgacggcgg aagatcctta cttcaaagcg caggtcgtcc tcttacagga
      721 agaagaagcg ccgaaggaac aagattttga agcctatgtc tccaatatca aagacctggc
      781 ttcacagatc atccagcttt cgccgaatat tccttcggaa gccagtatca ttttaaagaa
      841 tattgagaac ccctccttcc tcatccactt catctcgagc aacctcaata cggagctgca
      901 cgagaagcag cagctgctcg aaatccacca tatcaaatcg cgggcggatc tcctgatgca
      961 gctcttgcag cgcgagcttc agttcgcgga gctgaagaat aagctgacca accggacgaa
     1021 gacggagctc gataagcagc agcgggaata tttcctgcag cagcagctga agagcattaa
     1081 ggatgagctg ggcggggaca gcaacgagcg ggaggtaaag gagatgcaga ag
//
LOCUS       DATMKH010000683         1069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1277486, whole genome shotgun sequence.
ACCESSION   DATMKH010000683 DATMKH010000000
VERSION     DATMKH010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1277486"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1069
                     /locus_tag="VNV63_08125"
     CDS             <1..>1069
                     /locus_tag="VNV63_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010913403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXC62627.1"
                     /translation="GKSTLMKVLSGVYPHGTYSGEIHYQGQERHLRGISDSEKLGIII
                     IHQELALVPLLSITENIFLGNEQAKNGVIDWDAAEKRTHGLLQKVGLNENPETLITNL
                     GVGKQQLVEIAKALSKEVKLLILDEPTASLNENDSDALLALLLEFKQHGITSILISHK
                     LNEVKKVADQITILRDGATIETLDTHEISEARIITSMVGRELVDRFPKRTPNIGEPIF
                     EVKDWVVHHPIHADRLMIKGVNLQVKKGEIVGIAGLMGSGRTEFAMSVFGRSYGRRIS
                     GEVFLHGKKIDVSTIGKAVDHGIAYVTEDRKTYGLNLIDHIKHNITIVNLKGVSKAGV
                     IDDLQELGVANEYRAKTNIRSS"
BASE COUNT          286 a          265 c          293 g          225 t
ORIGIN      
        1 tggcaaatcg actctgatga aggtgctaag cggagtttat ccccacggca cctactcagg
       61 ggagattcat tatcagggtc aggaacgcca tctccgaggt atctcggaca gtgagaagtt
      121 aggaattatc attattcacc aggaacttgc actggttcct ctcttatcca ttaccgagaa
      181 tattttcctc ggcaacgaac aagcgaaaaa cggggtcatt gactgggatg cagcggaaaa
      241 gcgcacccat gggcttctcc aaaaggtggg gttaaatgaa aatccggaaa ccctgatcac
      301 caacctggga gttgggaaac agcaattggt cgagatcgcc aaggctcttt ccaaagaagt
      361 taagctgctg attctcgatg aaccgacggc cagcctgaac gaaaacgata gcgacgcctt
      421 gctcgcactc ttgcttgaat ttaagcagca tggaatcacc tcgatcctga tttcccataa
      481 gctgaacgaa gttaaaaagg tggcggatca gatcaccatc ctgcgcgatg gagcgaccat
      541 cgaaacgctg gacacccacg agatcagcga agcccggatt atcaccagca tggtcggacg
      601 cgagctggtt gaccgtttcc ccaaacgaac tccgaatatc ggtgagccga tttttgaagt
      661 gaaggattgg gtggttcacc accctattca tgccgatcgg ctaatgatca agggcgtcaa
      721 cctgcaggtg aagaaggggg agatcgtcgg gatcgcggga ttgatgggct ccggccggac
      781 cgagttcgcg atgagtgttt tcggcagatc ttacggacgc cggatcagtg gcgaagtgtt
      841 tctgcacggc aagaagatcg atgtcagcac catcggcaag gcggtcgatc acggcatagc
      901 atatgtcacg gaagaccgca aaacctacgg tctgaacctg attgaccaca tcaagcacaa
      961 catcacgatc gtcaatctca agggtgtctc gaaggctggt gtgatcgacg atttgcagga
     1021 gctgggggtt gccaacgaat atcgggccaa aaccaatatc cgttcgtcg
//
LOCUS       DATMKH010000684         1337 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1277521, whole genome shotgun sequence.
ACCESSION   DATMKH010000684 DATMKH010000000
VERSION     DATMKH010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1277521"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
BASE COUNT          257 a          342 c          352 g          386 t
ORIGIN      
        1 ttgcttattt ttttgctaag atgaaatcaa aaactccaat ccatcctccg attatcgtcc
       61 ccacagtccc cacagtccac acgtctccac acgtctccac acgtctccac aagtcaaaag
      121 aatgtcagaa tattaatact gtaaacgata acaaacaaac acatgtcctc cacaaaatgt
      181 ttaccatcga ggtctttttg cttcgttatc tcaatcaatc atcatcaccc atccccgcag
      241 tcaactcctg aacccgtttc cttatcctcg caaccctcgc gtcaacactc tcctcatcct
      301 cctcatccgg tctcaaaggc cccctccccc ccaacttcct cggcggactc cctcccacgc
      361 cgccagtaac ctgtcttgtc ctcggaatat tcacctctgc aacttcggaa gaatctccat
      421 tcttgggcgg cgcaacgaca cgattcaacg cgcgttgacc tgctgctttt cgaatttgtg
      481 cccaacggtc catttcgtcg tctgaggatg tttgttctcg ggtcggggct acagaacgca
      541 cggcgggagc gcgggcgggg gggtcgagat ggtcttcttt gggtgtggat ttcgggaggt
      601 aggtttcggg tttggggaga tcaggttttg tactggaggg agggatccag acttcggggg
      661 ttgaaggatg ggattcaacg cggtggattc catcgaagtc agagtacaat tccttagagt
      721 cgacgatggg agcgggactt ggatctcgtg cggttggttt ctgtacgatg gggggaagag
      781 gacttcgtgc gcgatcttgc gattgaggtg gagtttgcgg tgggatagga gacattgccc
      841 tcgaggcccg tttggcttct ttttcctctt ttgttttccc gccaaagagt tttttgagtt
      901 tattcggttg cgatttcttg ggttcgggcg ttagactcct gccatatgcg gtatctggcg
      961 acaaaagaac gggaccttga accggttccg gggatttcga tctttgaagt tggacttttg
     1021 gagcgactgt aggttctcga ggaatttctt ggagaacatc gattttttcg gggattttcg
     1081 gctcgacttt tggtgatggc tcgactttaa taggtttcgg ttgcggcgtc ggtgccggtg
     1141 cttgtggtgg gggtggtggt ggtggaggaa gttccaccgg tttcggtttc tcgataatcc
     1201 tggatctttc aaatgttttg gcatcaaggc ctccctccgc ccacctcatt gctctggaaa
     1261 tatctgctcc aagaggcatc atactggtac ttggtcgccc gttatattcc gcactccttc
     1321 tcctttgcgg aggcggt
//
LOCUS       DATMKH010000685         1049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3188938, whole genome shotgun sequence.
ACCESSION   DATMKH010000685 DATMKH010000000
VERSION     DATMKH010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3188938"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="VNV63_08130"
     CDS             <1..398
                     /locus_tag="VNV63_08130"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXC62628.1"
                     /translation="EVLKNIQGDTDENTMLWIPEQRILIAGDVLFNNMHVYTAETDSK
                     AREKWLKSLNKIRTLKPSVVIPGHSKVGAPLDASSAVDFTENYLLVFEEELKKAKDAD
                     SLINAMKEKFPSADLLLAIERGAKANVKP"
     gene            482..790
                     /locus_tag="VNV63_08135"
     CDS             482..790
                     /locus_tag="VNV63_08135"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HXC62629.1"
                     /translation="MKKKRKHYTPEEKVAILRRHLVEGVPISDLCDEQGLQPTVLYRW
                     QKEFFENGAAAFLPRGRTNHQPEQERIAYLEKKIQTKDEVLAELMAEHVALKKRVGEL
                     "
     gene            829..>1049
                     /locus_tag="VNV63_08140"
     CDS             829..>1049
                     /locus_tag="VNV63_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HXC62630.1"
                     /translation="MDFVRRWSEKTEIGAGRFIGWLDITASKFYGWRERYGRVNEHNR
                     WVPRDFWLEDWEKQAIIAFHLKNPLEGYRR"
BASE COUNT          300 a          239 c          299 g          211 t
ORIGIN      
        1 tagaggtcct taaaaacatt caaggcgaca ccgatgaaaa cacaatgctt tggattcctg
       61 aacagagaat tctcattgcc ggagacgttt tattcaataa catgcatgtc tacactgcag
      121 aaacggacag taaggctcga gagaagtggt tgaagtcatt gaataaaatc agaacattaa
      181 aaccttcggt tgttatccct gggcatagca aggttggagc tccccttgac gcgagtagcg
      241 ccgtagattt tacggaaaac tatttattag tttttgaaga ggaactcaaa aaagccaaag
      301 atgcggatag ccttattaat gctatgaaag aaaaatttcc gtccgccgac ttgctcttgg
      361 caatcgaacg gggcgccaag gctaatgtta aaccctgaca aacaaactga cgatgcgcag
      421 ctgtcctgcc tcagctggaa atggagaaag cgttcatccg ttaagataac ggagcgaaca
      481 cataaagaaa aaacggaaac actacacgcc ggaagagaaa gtcgccattc tcaggaggca
      541 tttggtggaa ggagtgccga tctcggatct gtgtgacgaa cagggactcc agccaaccgt
      601 gctctaccgc tggcagaagg agttcttcga gaacggagcc gcagctttcc tgccgagagg
      661 tcggaccaac catcagccag agcaggaacg aatcgcgtat ctggagaaga agattcagac
      721 gaaggatgaa gttttggccg aactgatggc ggagcatgtt gccctaaaaa aaagagttgg
      781 ggaactctga ccggggggtg gattccgcac gatacgcagg accagatcgt ggatttcgtc
      841 cggcgctggt cggagaagac cgagatcggc gccggacggt tcatcggatg gctggacatc
      901 acggccagca agttctacgg ctggcgcgag cggtacgggc gtgtcaacga gcacaaccgc
      961 tgggttcccc gggacttttg gctggaggat tgggagaagc aggcgatcat tgcgttccac
     1021 ctgaagaatc cgctggaagg ctaccggcg
//
LOCUS       DATMKH010000686         2120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3824573, whole genome shotgun sequence.
ACCESSION   DATMKH010000686 DATMKH010000000
VERSION     DATMKH010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3824573"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            199..2049
                     /locus_tag="VNV63_08145"
     CDS             199..2049
                     /locus_tag="VNV63_08145"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011189521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HXC62631.1"
                     /translation="MPPCHSPDLNFMSSKKHNYDESKIKTLSSLEHIRTRTGMYIGRL
                     GDGSHYDDGIYVLIKEVIDNAIDEFIMGYGKVVDISLNTNVVRVRDFGRGIPLGKVIE
                     CVCRINTGAKYNDEVFQFSVGLNGVGTKAVNALSDTFTVTSYRDRQFFRADFSRGELK
                     SQKKGKQNEPDGTEIVFEPDPTIFKSFRFREEHLLRRLHLYTYLNAGLRLKFNGQVFT
                     SENGLKDLLEEDVGEEPLYPVLHFREKMIEIAFTHTNRLSEEHYAFVNGQYTADGGTH
                     LSAFREGMLKAINDVAKAKFEGDDVREGLVGAIAIRLKDPVFESQTKNRLGNTEIRTE
                     LVAKVREIVATLLHKNSEVTERLLTKIQETQRLRKELQDVRKLARERSAAISFRIPQL
                     KDCKVHLNLEKGIGKESMIFITEGQSAAGSITSCRDPNVQAVFTLKGKPLNVWDLNRT
                     IVYKNDEIFNLMRTLNVEENLQNLRYGRVILATDADVDGLHIRNLLITCFLRFFPDII
                     TAGHVSILETPLFRVRDKKQTFYCYSEAERDEAMRKIKNPEITRFKGLGEISPSEFKA
                     FIGPAMKLTHVSVDDGHVVQPLLQFYMGKNTPARRQFIMDHLVVEEPVGA"
BASE COUNT          537 a          569 c          536 g          478 t
ORIGIN      
        1 ctgaccccct cgtaaccttc accaggtttt gcgcgggctc gcccgcaatc tgtcatttgt
       61 catttgtcat ctgtcattta ggtccagccg cgcccttccg gtttctccga ttttgggcta
      121 tattacccgc gatctcgaat ctcgtttttt actctttaaa ctccgaaccc aacccgcttg
      181 cagcgtcttg caagctggat gcctccgtgt cactctcccg atcttaattt tatgtcgtcc
      241 aagaagcaca actacgatga gagcaagatc aaaaccctct catccctcga gcacatccgg
      301 actcgaaccg gtatgtatat cggccggctt ggagacggca gccattacga tgacggcatc
      361 tatgtcttga tcaaagaggt gatcgataac gccattgatg agttcatcat gggatacggc
      421 aaggtggtcg atatctcgct caataccaat gttgtgcggg tccgggattt cggtcgcggt
      481 atcccattag gcaaagtgat cgaatgcgtt tgccggatta acactggagc caaatataac
      541 gacgaggtct tccaattcag cgtcggtctc aatggcgtcg gtactaaggc ggtcaacgcc
      601 ttgtccgaca cctttaccgt caccagctat cgagatcgcc aattctttcg ggccgacttc
      661 agccggggtg agctaaagtc gcaaaagaaa gggaaacaga acgagccgga cggtacggag
      721 atcgttttcg agcccgaccc gaccatcttt aagagtttcc gtttccggga agaacatctg
      781 ctgcgccggc ttcacctcta cacttaccta aacgccggtc tcaggctcaa attcaatggt
      841 caggtgttca cctcggagaa cgggctaaaa gacttgcttg aggaagatgt gggcgaagaa
      901 ccgctgtatc cggtgctgca ttttcgggaa aagatgatcg agatagcgtt tactcacacc
      961 aatcgattat cggaagagca ctacgctttc gtgaacggtc aatataccgc agacggcggc
     1021 acgcatttga gcgcctttcg agagggcatg ttgaaggcga tcaacgacgt ggccaaagcg
     1081 aagttcgagg gagacgatgt gcgcgaagga ttggtgggtg cgatcgccat ccggctcaag
     1141 gatccggtgt tcgaatccca aaccaaaaat aggctgggga ataccgagat ccgcaccgag
     1201 ttggtggcta aagtccgtga gattgtggcc acgctgctgc acaagaatag cgaagtcacc
     1261 gagcggttac tgaccaaaat ccaggagacc cagcgtctgc ggaaagagct acaagatgtc
     1321 cggaaactgg ctcgcgaacg ttccgccgcg atctcgttcc gcatccctca gctcaaagat
     1381 tgtaaggtcc acctgaactt ggaaaaaggg atcgggaaag aatcgatgat ctttatcacc
     1441 gaaggccaat cggctgccgg ctcgatcact agttgccggg atcccaacgt ccaagctgtc
     1501 tttacgctga aaggcaagcc gctcaatgtt tgggatctca accggaccat cgtttataaa
     1561 aacgacgaga tcttcaattt gatgcggacc ttaaacgtag aagaaaacct gcagaatctg
     1621 cggtacggca gagtgatcct agccaccgat gccgacgtgg acggactcca cattcgcaac
     1681 cttttaatca cctgctttct gcgatttttc ccggacatca tcactgccgg ccacgtttct
     1741 atcttggaaa caccgttgtt tcgggtgcga gacaagaagc aaaccttcta ttgctactca
     1801 gaagccgaac gcgatgaagc catgcgaaag attaagaacc cagagatcac ccgattcaaa
     1861 ggcttgggag aaatctctcc atcagaattc aaggcattca tcggtccagc gatgaagcta
     1921 acccacgtct cagtcgacga cggccacgtg gtccagcctc tcctgcaatt ctatatgggc
     1981 aaaaacactc ccgcccgtcg ccagttcatt atggatcacc tcgtggtcga agaacccgtg
     2041 ggggcatgaa ttccggccgc ctttgaatct cacacaaaga cacaaaggca caaaggttaa
     2101 gaggtaagaa tggttgccgg
//
LOCUS       DATMKH010000687         2140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4486323, whole genome shotgun sequence.
ACCESSION   DATMKH010000687 DATMKH010000000
VERSION     DATMKH010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4486323"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1025
                     /gene="fabF"
                     /locus_tag="VNV63_08150"
     CDS             <1..1025
                     /gene="fabF"
                     /locus_tag="VNV63_08150"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012544941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HXC62632.1"
                     /translation="FIHYAVAASQMVMDDSGLKVSDDNRERIGVIVGSGIGGLQAIEE
                     WHKVLLEKGPRRVTPFFIPMTIINLASGQIAIRFGVQGPNSSSVTACATGNHCIGDAF
                     RIIQRGEADAMLAGGTESAVTPLSVAGFAASRALSSRNDDPMHASRPFDRDRDGFVLG
                     EGAGVVLLEELETAKRRGAKIYAEVAGYSMTGDAYHITAPPENGEGAIRCMRLALKDA
                     GIFPSEIGYIKAHATSTMADAIETRAIKAVFEKQAYRIPVSAPKSMVGHLLGAAGGVE
                     AILTTLAIHRGILPPTINYEHPDPECDLDYIPNKARKASISSALSNSFGFGGTNACLI
                     FKAYSL"
     gene            1039..1770
                     /gene="rnc"
                     /locus_tag="VNV63_08155"
     CDS             1039..1770
                     /gene="rnc"
                     /locus_tag="VNV63_08155"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HXC62633.1"
                     /translation="MSSFELDQLQEKLSYRFKRPDLLRQAITHKSHLNENRDRVQQDN
                     ERLEFLGDAVLDLVVAELLINRFPGATEGELSKAKAQLVSEPMLSQIARQIEIGKFVL
                     LGKGEELTQGREKASILSDTLEAIIAAFYLDGGFSTAKTFIARCFEEPIRDLAEPSSA
                     ESVRSPYSDYKTRLQELCQKEFETLPVYELFRESGPDHQKRFEIQLFIAGRVISQGVG
                     RTKKEAEQMAAKEALKQLEKKRVSD"
     gene            1819..>2140
                     /locus_tag="VNV63_08160"
     CDS             1819..>2140
                     /locus_tag="VNV63_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HXC62634.1"
                     /translation="MGRLTYQKAGVNIAEGQSFVRKIRPLVRSTFRPEVLTDLGGFSA
                     LFRLDLKKYREPILVSGTDGVGTKLKIAFLMNRHNTVGIDLVAMCVNDVVVTGAEPLF
                     FLDYF"
BASE COUNT          495 a          529 c          601 g          515 t
ORIGIN      
        1 ccttcatcca ttatgccgtg gcggccagcc agatggtaat ggatgattcc ggactcaaag
       61 tatcggatga taaccgggaa cgcattggcg taattgtggg ttcggggatc ggggggctcc
      121 aggcgattga agaatggcac aaagtcctgc ttgagaaagg tcctcgacgg gttacccctt
      181 tctttatccc gatgacgatt atcaaccttg cctcgggtca gatcgcaatc cgattcggcg
      241 ttcagggccc gaactcctct tccgtaaccg cctgcgcaac cggcaaccac tgtatcggag
      301 atgcctttcg aatcatccag cgcggcgagg cggatgcgat gctggcaggg ggaaccgaat
      361 cggccgtgac gccgttgtcc gtggccggtt ttgcggcttc tcgtgcgctt tcctcacgga
      421 acgatgatcc gatgcacgcc agccgtcctt ttgatcggga tcgggatggt tttgtactgg
      481 gagaaggagc gggtgtcgtg ttgctggaag agcttgagac agccaaacgg agaggcgcta
      541 agatctatgc cgaggtggca gggtattcga tgactggcga tgcctatcat attaccgccc
      601 ctccggaaaa tggcgaagga gcgatccgat gcatgcggct tgcgctaaaa gatgccggaa
      661 tttttccatc ggagataggt tacatcaagg cccacgccac ctccacgatg gcggatgcga
      721 tcgagactcg cgcgatcaag gcggtgtttg aaaaacaggc ctaccggatt ccggtcagtg
      781 caccgaaatc tatggtcggt catctcttgg gtgccgccgg aggcgtcgag gcgatcttga
      841 cgacgctggc aattcatcgc ggcatacttc ctccaacgat caactacgag catcccgacc
      901 cggaatgcga cctggattat attccgaaca aggcacgcaa ggcgtccatt tcctcagcct
      961 tatctaactc ttttggtttt ggcggcacca acgcctgtct gatctttaag gcctattcgc
     1021 tttgatcacc cattttagat gtcttccttc gagttagacc aactccaaga gaagctctca
     1081 taccgtttca agcgtccgga tcttctccga caagcgatta cacataaatc acatctcaac
     1141 gaaaatcggg accgggtgca acaggataac gagcggctgg aatttttagg cgatgcggtt
     1201 ctggaccttg tggtggctga gcttctaatc aaccggttcc ccggggcaac cgagggagag
     1261 ctctctaagg ccaaggcgca actggtcagc gaaccgatgc tgagtcagat cgcaaggcag
     1321 attgagatag gaaagtttgt ccttttgggg aaaggggagg agctcaccca ggggagggag
     1381 aaggcctcca tcctttcaga caccttagag gcgattattg cggctttcta tctcgatggt
     1441 ggtttttcaa cggcgaaaac atttattgcc cgatgttttg aggaaccgat ccgtgatctg
     1501 gccgaaccga gtagtgccga atccgttagg agtccttatt ctgattacaa gacaagactg
     1561 caggagctgt gtcaaaagga gtttgaaaca ctcccggtgt atgaactttt ccgcgaatcc
     1621 ggtccagatc atcaaaaacg atttgagatt cagcttttta ttgcagggcg agttattagt
     1681 cagggagtgg gtcggacaaa gaaagaggcg gagcagatgg cagccaaaga ggcgctgaaa
     1741 cagcttgaaa agaagcgagt gtccgattga ccgcttggct tcttcctgat atcatattct
     1801 taaatcgatg aaatttgtat ggggcgacta acttatcaga aggccggtgt gaatattgcc
     1861 gaaggccaat cttttgtgcg taaaatcagg cctctggtac gctcgacctt tcgcccggag
     1921 gtgctcaccg acttaggcgg tttcagcgcc cttttcagat tggaccttaa aaaatataga
     1981 gaaccgattc ttgtctctgg gaccgatggg gtcggcacga agctcaagat cgcctttctc
     2041 atgaaccggc acaatacagt cggcattgat cttgtggcga tgtgtgtcaa tgatgtggtg
     2101 gtcaccggtg ccgagccact cttctttctg gattactttg
//
LOCUS       DATMKH010000688         1125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1600069, whole genome shotgun sequence.
ACCESSION   DATMKH010000688 DATMKH010000000
VERSION     DATMKH010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1600069"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1054
                     /locus_tag="VNV63_08165"
     CDS             <1..1054
                     /locus_tag="VNV63_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62635.1"
                     /translation="LNIKWGAQCTLFLGSEPDMVKLAADSGCVAMFVGLESISEDVLT
                     EMNKPFNKEKKYEDQIKCFHDHGIMVNPGIIFGADGDDESVFENTVEFLVKNRVELAY
                     INISTPLPGTALFDRLKADGRIFDWDWSKYDGKHVVYYPKRMTPEVLQEGFYWANRQF
                     FSIPSIATRMSSTSQRFMARWMMNWRFRHLIYRTCPKGRISPLSRVIKTLHDRLPSIE
                     RDQFIPNALHALKEKVETVSVQIDRFLQIKVKKSESQQSLTVELDGTLDRLNVKELKK
                     RIEKAADKAKLEIIVNFENLKHATPAALQMLLEGDYLKKVGPYAKIKYRNLQSSFRAE
                     IDSLVHSGVKIPQEDP"
BASE COUNT          296 a          275 c          313 g          241 t
ORIGIN      
        1 gctcaatatc aaatggggcg cccaatgcac gctcttctta ggcagcgagc cggacatggt
       61 aaagctggcg gccgacagcg gttgtgtggc gatgttcgtc gggttggaat ccattagtga
      121 agacgtcttg accgagatga acaagccgtt caataaagaa aaaaagtatg aggaccagat
      181 caagtgtttc catgatcatg gaatcatggt gaacccggga atcatctttg gcgcggacgg
      241 ggatgatgaa tcggtgttcg aaaacacggt ggagtttctc gttaagaacc gagtggaact
      301 ggcatacatc aatatctcaa cacccctgcc gggcaccgcg ctgttcgacc gattgaaggc
      361 cgatgggcgc atcttcgatt gggactggag caagtatgac gggaaacatg tggtttatta
      421 ccccaagcgt atgacgcccg aggtcctgca ggaaggtttc tattgggcaa accgccaatt
      481 cttttccatc ccttccattg ccacgcggat gtcttccaca agccaacggt tcatggcgcg
      541 atggatgatg aactggagat tccggcatct catctatcgg acctgcccca agggacggat
      601 ctcgccttta agccgtgtga tcaagacact gcacgatcgg ctcccttcca tcgagcggga
      661 tcaatttatc ccgaatgcgc tgcacgcttt gaaggagaag gtggagactg tatctgtaca
      721 gatcgaccgg ttcctgcaga tcaaggtcaa aaagagcgag tctcagcaga gcctcacggt
      781 ggagctggac ggcacactgg atcgcctgaa cgtcaaggaa ctcaagaaac ggattgagaa
      841 agcggctgac aaagccaagc tggagatcat cgtgaatttt gagaacctca agcatgccac
      901 gcccgccgcg cttcagatgc tgttggaagg tgattacctg aagaaggtgg ggccgtatgc
      961 caaaatcaag taccgcaacc tccagtcctc gttccgggcg gagatagata gcctcgtgca
     1021 ttcgggagta aagattcccc aggaagaccc gtaagcaaaa tgcatcagtt tatctatcaa
     1081 ggaaaagagc tttactgtga aggggtcccg attcgaaaaa tcgct
//
LOCUS       DATMKH010000689         1028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1600241, whole genome shotgun sequence.
ACCESSION   DATMKH010000689 DATMKH010000000
VERSION     DATMKH010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1600241"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /locus_tag="VNV63_08170"
     CDS             complement(<1..583)
                     /locus_tag="VNV63_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62636.1"
                     /translation="MSSSISSSEDPTAVIHSSFFAQGLVLIAITIAVTLALGHATVLL
                     HHPDPVIPSRSDLSTADHLVVAFGNSRFFANIDFDELGKQLSKPGDDVKVSDFTSGGW
                     DSLHYYMLALLYKDVLRPGRDAVLIEVSPLSEDDNESANYLDVIRPSLAWRVAELPGI
                     PLESRLDILLGAVADLYRFRGRIQYFKLAPRLGH"
     gene            complement(558..>1028)
                     /locus_tag="VNV63_08175"
     CDS             complement(558..>1028)
                     /locus_tag="VNV63_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HXC62637.1"
                     /translation="MITMLLGGLWHGASWTFMVWGGLHGLCLSVERLLGVNVQGPTGK
                     IARVGRQILTFHLVCLGWIFFRNRTFQSAGEMLRGIFAAKFWISPPELFPTLGAVVLI
                     ILYCVFSYAGRHLTLPDMEQSWPRQIAVAVGCSLVLIAFMVFGASSNVFIYFQF"
BASE COUNT          275 a          273 c          277 g          203 t
ORIGIN      
        1 cgtgtccaag acgcggggcc agtttgaagt attgaatcct tccacggaag cgatacagat
       61 cagcaactgc ccccaaaagg atgtcaagcc tagactcgag agggattccg ggaagttcgg
      121 cgaccctcca ggcaagcgaa gggcggatca catccagata gttggctgat tcattgtcat
      181 cttccgagag gggagatacc tctatgagca cagcatcccg tcctggcctc aacacatcct
      241 tgtagagtag cgccagcatg tagtaatgga gcgagtccca tcctccactc gtaaaatcgc
      301 ttaccttcac atcatcgccc ggtttgctca gttgcttccc gagttcgtcg aaatcgatat
      361 tggcgaaaaa ccgagagttt ccaaaggcga ccaccaaatg atcggcggtg cttagatcgg
      421 atcgacttgg aatgacggga tccggatggt ggagcagcac agtagcgtga cccagcgcaa
      481 gtgtcacggc tatcgtgatg gcgatgagca cgagcccttg cgcaaaaaac gatgaatgga
      541 tgacggcggt cggatcttca gaactggaaa tagatgaaga catttgagct cgctccaaag
      601 accatgaagg ctatcaggac tagactgcat ccaacagcaa cggcgatttg tctaggccaa
      661 ctttgttcca tatcgggaag cgttagatgc cttcccgcgt atgagaacac acaataaagg
      721 ataatgagga ccacagctcc gagcgtggga aacagctcgg gcggactaat ccagaacttt
      781 gctgcaaaaa tgccccgtaa catttcgccc gcagattgga atgtccgatt gcgaaagaag
      841 atccatccca ggcaaaccag gtggaaagtt agaatctgcc tgccgacacg ggcaatcttt
      901 cctgtcggtc cttgcacgtt tacgccgaga aggcgttcca ccgagagaca aagtccgtga
      961 aggcctcccc agaccatgaa cgtccaggag gcgccgtgcc agaggccgcc cagcagcatg
     1021 gtgatcat
//
LOCUS       DATMKH010000690         1297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4781844, whole genome shotgun sequence.
ACCESSION   DATMKH010000690 DATMKH010000000
VERSION     DATMKH010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4781844"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="VNV63_08180"
     CDS             complement(<1..295)
                     /locus_tag="VNV63_08180"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF015647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HXC62638.1"
                     /translation="MKVIGLISGTSTDGIDAALVQITGLGLQAKLRLIAFEAYPYPPK
                     LRRQLLDVAQKGRVDEICHLNFYLGELFAQAAIKVAAKAGVARREIDLIGSHGQ"
     gene            614..>1297
                     /locus_tag="VNV63_08185"
     CDS             614..>1297
                     /locus_tag="VNV63_08185"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HXC62639.1"
                     /translation="MIKIDRRIVHRLFWGCMGALLLLSFEARAQAEPPLKIVVTLPVL
                     KDLTEQIGREHVVVRSLITELESEYTYSPTSNDILAVNEARIFFQIGIGLEVWVDGLI
                     KNAGNKNLLVVTTGQGVPLLRDPGLEESGSNTPPLNRHRLGNPHIWLDPENAKTILRH
                     ITDGLIKVDSKSKRVYLHNQAEYLKKLDFLEAGLKQKTSKLIHRKIVTYDPSWPYFAR
                     RFGFEIAGSL"
BASE COUNT          328 a          343 c          340 g          286 t
ORIGIN      
        1 tctgcccatg agagccgatc aggtctatct ccctccgggc aacgcccgct ttggccgcaa
       61 ccttgatggc cgcctgggca aagagttcgc cgagatagaa attgagatga cagatctcat
      121 cgacccgtcc cttctgtgcc acatctaaaa gttgccggcg aagcttggga ggataagggt
      181 aggcctcaaa cgcgatcagc cggagcttgg cttgaagccc caggcctgtg atctggacca
      241 gagcggcgtc aatcccgtcc gtggatgtcc ctgaaatgag tccgatgacc ttcatgcgag
      301 atagggtttt aaatccgtcg tagcgtagcc tattctgaaa accccgtcaa gcggaaaccc
      361 gtggcatttg cacatgcccg aagccaaggg gtgaaggaac agaccccgcg cttgataacg
      421 atgatagact caaaccaccg ccagggatgc ggtaacggcc gcgtccctgg ccaatacgaa
      481 aacggtcaaa ggctcgatct cccatgaacg catggggtgt gtgacggaat cccttggcga
      541 gggtctgagc actgccagat ggtcgaattg acatagagtt gccttaatgc tatcttgtgg
      601 cggtttgcta acgatgataa agatagatag aaggatcgta caccgcctct tttggggctg
      661 tatgggcgca ttgcttctct tatcttttga agcaagggcc caagcggagc cgcctcttaa
      721 gatcgtcgtc acactccccg tcttaaaaga tttaaccgaa cagatcggaa gagaacatgt
      781 ggttgtccga tcccttatca cggaacttga aagcgaatac acctattctc ctacctctaa
      841 tgatatcctc gcggtcaatg aggcccggat tttctttcag atcggaattg gcctcgaggt
      901 ctgggtggac ggtctgatca agaatgccgg gaataagaat ttactggttg tcaccacagg
      961 tcaaggcgtc cccctcctcc gagatccggg attggaagag tctggatcta acacccctcc
     1021 gcttaaccgg catcggctcg gcaaccccca catctggctc gacccggaga acgccaagac
     1081 gatactgagg cacatcacgg acggcctgat caaagtagac tcaaaaagca agagagttta
     1141 cctccacaac caggcggaat acctgaagaa gcttgatttt ttggaggcgg gactaaaaca
     1201 aaaaacttct aaattgattc atcgaaaaat tgtgacctac gatccatcct ggccgtattt
     1261 tgcaagacgg ttcgggtttg aaatcgccgg gagcctt
//
LOCUS       DATMKH010000691         1023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2223996, whole genome shotgun sequence.
ACCESSION   DATMKH010000691 DATMKH010000000
VERSION     DATMKH010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2223996"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1023
                     /locus_tag="VNV63_08190"
     CDS             <1..>1023
                     /locus_tag="VNV63_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014406555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HXC62640.1"
                     /translation="PVGLVTPETDQQETKRAYVYDPHLDPALKWAGKAERTSFEVPTV
                     SLHVHERIDPRTILEAVRKKNGSNYEQMSLFSSRAENPPLREAIEFYKHAHGWSNRLI
                     AGDSLLVMNSLLEKEGMAGHVQMIYFDPPYGIKYGSNFQPFVNNRDVKDGKDEHLTAE
                     PEMIKAFRDTWELGIHSYLSYIRDRLFLSRELLHESGSIFVQISDENIHHVREILDEV
                     FGAKNFCGQIPFSKTGGQADLRLASVADYLLWYAKDRSSVKYRQLFLNKERGGTGSGE
                     YSWVDLPNGERRRITKEENDDTGLLPPGAKFFIPDNLTSADVTSTGTYTYEFDGRKFF
                     PKGGHW"
BASE COUNT          251 a          272 c          277 g          223 t
ORIGIN      
        1 gccggtaggc ttggtgacac ctgaaacgga ccaacaggaa acaaaacgag catacgttta
       61 cgatccgcac ctcgaccctg ccttgaagtg ggcaggaaag gcggagcgaa ccagcttcga
      121 agtgccgacg gtttcgctcc atgttcacga gcgaatcgat ccgcgcacta ttcttgaagc
      181 agttcgcaag aagaacggca gcaactacga gcagatgtcc ctcttcagct cccgcgctga
      241 gaacccaccc ctccgcgagg ctatcgagtt ctacaagcac gcccacggct ggagcaacag
      301 gctgatcgcc ggcgacagcc tgctcgtgat gaattcgctt ctcgaaaagg aaggcatggc
      361 aggccatgtc cagatgatct acttcgatcc gccgtatggc atcaagtacg gatctaattt
      421 ccagcccttt gtgaacaacc gggacgtgaa ggatggaaag gatgaacacc tgacagcgga
      481 gccggagatg atcaaggcgt tccgcgacac ctgggagctg ggcatccatt cttacctaag
      541 ctatatccgc gatcggcttt tcctctctcg cgagctgctt cacgaatccg gttccatctt
      601 cgttcagatc tccgatgaaa acattcacca tgtccgtgaa attctggatg aagtgtttgg
      661 agccaagaat ttctgcgggc agataccttt ctccaaaacc ggcggtcagg ctgacctcag
      721 gttggcgtcc gttgcggatt atctcctctg gtacgcaaaa gaccggtcat ccgtgaagta
      781 tcggcagttg tttctaaaca aggaacgggg cggaacaggg tccggcgagt actcctgggt
      841 tgacttgcca aacggcgaaa gaaggcggat caccaaggaa gagaatgatg atacgggcct
      901 tcttcctccc ggtgcaaaat tctttatccc tgataatttg acatcggctg atgtcacttc
      961 gaccggtacg tatacgtatg agtttgatgg gaggaaattc tttccgaagg gcggtcattg
     1021 gaa
//
LOCUS       DATMKH010000692         1392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3827005, whole genome shotgun sequence.
ACCESSION   DATMKH010000692 DATMKH010000000
VERSION     DATMKH010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3827005"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..284
                     /locus_tag="VNV63_08195"
                     /pseudo
     CDS             69..284
                     /locus_tag="VNV63_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008116574.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
     gene            623..778
                     /locus_tag="VNV63_08200"
     CDS             623..778
                     /locus_tag="VNV63_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HXC62641.1"
                     /translation="MDLLWTLAVILLVLWLLGLVTSYTLGGFIHILLVVALIVVLIRV
                     IQGRRPV"
     gene            1040..>1392
                     /locus_tag="VNV63_08205"
     CDS             1040..>1392
                     /locus_tag="VNV63_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013578696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YXWGXW repeat-containing protein"
                     /protein_id="HXC62642.1"
                     /translation="MRIIFSIRSLLLTLGVLAISAASFAQIGVGVSITIAPPDLPVYE
                     QPLCPGEDYIWTPGYWAYADDDYYWVPGTWIVAPEVGFLWTPGYWAFEENRYFFHEGY
                     WGPEVGFYGGISYGFG"
BASE COUNT          344 a          347 c          393 g          308 t
ORIGIN      
        1 gttcctccca gcgggcacat gctcgaaccg ccacgcgaca aatgaaatta aatatttagg
       61 aggatccgat gaactgggat cagttggaag gaaaatggaa gcagtactcg ggcaaagcaa
      121 aggaaaagtg gggaaagctg acagacgatg atctcgacgt gattcgcgga cggcgagatc
      181 agttgatcgg aaagatccag gaacgctatg ggatcgtaag acaggaagca gagaaacaag
      241 tggatgagtt cgctcgggct ttccgtagcg aagactcaag gcaggaaaca cggcaaggaa
      301 atcagggaga gagtgatcac tcgacctcca acgagaagaa gcgcggtgcc ggacagcact
      361 gatttgagag ttgttagcag taacgcaaat caggatcgcg aggcgaaggg gcctggcgta
      421 ccgcgaatgt tgcccggctc ctccctcacg aaaacgcggt cactttaaga atcactacat
      481 cgaactatcc cgaaccctac gagaaacacc tgatggtccg atggtgcttt tccaagtacc
      541 ttctgccggt cgatattgat tccaggctca ggtccgccat cgcaacagct tggtcaataa
      601 ggatcgaact gaggagaaga aaatggactt actttggacg ctagcggtca tcctattggt
      661 gctctggctt ttggggctcg tcacttccta cacattggga ggattcattc acatcctgct
      721 cgttgtggcc ctcatcgtcg tactgatccg tgtcattcaa ggccgcaggc ctgtttagca
      781 ggcgcgttcc gacagccttt cataatcttt ggagtcctgt aaccgtagga aggttagtgc
      841 tcgcctatgc tcggggattc tctctcagcg cctacaggca taacccgatg gccctgcctt
      901 taacccagca atacgatgcc aacggtaccg aaggacgtta agcaagcttc gtccgaaaat
      961 cgcgcctttc aatcctcgca gcccgcagcg gcaaagaaag atcgggtaag cgaagaatga
     1021 ccgggcagaa ggaagtacaa tgcgtattat tttttccatt cgttcgctgt tgctcacgct
     1081 gggagtactg gccatctcgg cggcatcatt cgcgcaaatc ggcgtcggag tgtccattac
     1141 gatcgccccg cctgatttgc ccgtgtatga gcagccgtta tgcccggggg aagattatat
     1201 ctggaccccc ggttactggg cttacgctga tgacgattac tattgggtgc cgggcacctg
     1261 gatagtggcc ccggaagttg gttttctctg gaccccaggg tattgggcat ttgaagagaa
     1321 ccgatatttc ttccatgagg gctattgggg accagaagta ggattctacg gcggcatcag
     1381 ctacggattc gg
//
LOCUS       DATMKH010000693         2938 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766 k141_958131,
            whole genome shotgun sequence.
ACCESSION   DATMKH010000693 DATMKH010000000
VERSION     DATMKH010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_958131"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /gene="hemH"
                     /locus_tag="VNV63_08210"
     CDS             <1..868
                     /gene="hemH"
                     /locus_tag="VNV63_08210"
                     /inference="COORDINATES: protein motif:HMM:NF012964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HXC62643.1"
                     /translation="EPYLKDIRGGRPTSPELVEEIKERYRLMGGRSPLLEITHQQAQA
                     LEEALNKANSRRFRVYVGMRHWHPYIKETVDVMKQDGVKRVVGLCLTPYYSNMSVGAY
                     FKKLDEAQDEVGVKFKTSRVESWNDHPFLILAIAEKVEQALSKFPETIRGKVPILFTA
                     HSLPARIVEEKDPYPQELLETIEAVVKKVGHSPYKFAYQSQGRTPEPWLGPEAGTVIN
                     ELYDKGHRYLLMAPIGFISDHMETLYDVDILYRRQATAKGMRFERVESLNAAPLFISA
                     MADVVLKNLPPS"
     gene            895..2307
                     /gene="hemG"
                     /locus_tag="VNV63_08215"
     CDS             895..2307
                     /gene="hemG"
                     /locus_tag="VNV63_08215"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017952539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HXC62644.1"
                     /translation="MKTVAIVGGGITGLAAAYYLEEQAKTGVLPLTSRLIERLPQCGG
                     KIRTDRMNGFIIEGGPDSFISMKPWGIELCQRLGLSEHLTGTNPNQKQIYLLRKGRLA
                     ELPEGIMALMPTRLWPFLKSPLISPLGKLRMGLDLFIPPKADKQDESLASFVQRRLGK
                     EALERIAEPLLAGIYAGDPKQMSLASTFPQFREIEQKHGSLTRGMLARRRAVTPVSSR
                     YTMFMTLKEGMGELVETLTKRLDPSARITGSGAIGIQRRSTAYELLLADGRRLEADAV
                     ILATPAFVTAGLLKQIDPLLSDRLIKIPYVSTATVSLAYKRSGFSHPLNGFGFVVPRS
                     EKRKILACTWTSTKFPHRADSDHVLVRCFVGGAGREELALQDEGSLIKMVCEDLKAIM
                     GISAEPVLTKVYRWEKANPQYNIGHLERVAEIEKMTAQHPGLFVTGAAYRGVGVADCI
                     HQGLQTAEKVLAHLRFTKHV"
     gene            2361..2777
                     /locus_tag="VNV63_08220"
     CDS             2361..2777
                     /locus_tag="VNV63_08220"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXC62645.1"
                     /translation="MDKHDHIPLQGLRHLAIRVTDIQKARDFYQGLLGMKTVWEPDPE
                     NVYLSSGQDNLALHQIPNAELARYHQGIGQFMDHFGFLVENPEEVEEVFKKMETAGVR
                     IIKPVKRHRDGSISFYMTDPDKNVIQILYEPTISGI"
BASE COUNT          712 a          771 c          827 g          628 t
ORIGIN      
        1 cgagccgtat ctaaaagaca tacgtggggg tcgaccgacg agccctgagc tcgtggagga
       61 aataaaagaa cgatatcgcc tcatgggagg ccgctcgccg cttttggaga tcacccatca
      121 gcaggcgcag gccttggagg aggcgttgaa caaggcgaac agccggcggt tcagggtcta
      181 tgtcgggatg cgccactggc atccatatat caaagagact gtggacgtga tgaagcagga
      241 cggagtcaaa cgcgtcgtgg ggctctgcct tacgccttac tattcgaata tgagcgttgg
      301 ggcctatttt aaaaaattag atgaggccca agatgaagtc ggtgtgaagt ttaaaaccag
      361 ccgggtcgag agctggaatg accatccgtt tttaatcctg gcaatcgccg agaaggtgga
      421 acaggcgctc tcaaaatttc cggagacaat cagaggaaaa gtcccgatcc tgtttacagc
      481 ccacagcttg ccggcccgga ttgtcgaaga aaaagatcct taccctcagg agcttctgga
      541 aacgattgag gcggtagtca agaaagtcgg ccacagcccg tacaagttcg cttatcagag
      601 tcagggacgt acgccggagc cgtggctggg acccgaggcc ggcaccgtaa tcaatgagct
      661 atacgataag ggacaccgtt atctcctgat ggccccgatc ggtttcatct cggaccatat
      721 ggaaacactc tacgatgtgg atattctata ccgccggcaa gccacggcca aggggatgcg
      781 atttgagcgc gtcgagtcgc tcaatgccgc gccgcttttc atctcggcca tggcagatgt
      841 ggttttaaag aacctgcctc catcttaaaa taagaatacc ttaaggcgaa tcggatgaaa
      901 accgtggcaa tcgtaggagg aggcatcacc ggccttgcgg cggcatacta tctcgaagaa
      961 caggccaaga ccggcgtcct accgcttacc tcccgtctta tcgaacgcct gccgcagtgc
     1021 ggtgggaaaa tccgaacgga taggatgaat ggttttatca tcgagggggg accggattcg
     1081 ttcatctcga tgaagccctg gggaatcgag ctctgtcagc gcttgggtct ttccgaacac
     1141 ttgaccggca ccaaccctaa tcagaaacaa atctatctcc tccgcaaggg acgcctggca
     1201 gaactccccg aagggatcat ggcgctgatg ccgacacgcc tctggccgtt tttaaagagt
     1261 ccgctgattt cgccgcttgg caagctccga atgggtctgg atctctttat cccgcctaag
     1321 gcggacaaac aggacgagag tcttgcttca tttgtccaac ggcggcttgg caaggaggcg
     1381 ctcgagcgca tcgccgaacc attgctcgcc gggatctatg ccggtgatcc aaaacagatg
     1441 agtctcgcca gcaccttccc ccagtttcgg gagatcgagc aaaagcatgg aagcctgaca
     1501 cgcggcatgc tggccagacg ccgcgccgtg actcccgttt cttcccgcta cacgatgttt
     1561 atgaccttga aggaaggcat gggcgaactg gtcgagacgc tgaccaaacg tctggatcca
     1621 tcggcacgga taactggatc gggtgcgatt ggcattcaga ggcgctcaac ggcctacgag
     1681 cttctattgg cggatggccg gcgccttgaa gccgatgccg taatcctcgc tacgccggcc
     1741 tttgtaacag caggtcttct gaagcagatt gatccgctat tgtcggaccg gctcatcaag
     1801 attccgtatg tctcgaccgc aaccgtgtcg ctggcctaca aacgatccgg cttctctcat
     1861 ccgcttaacg gtttcgggtt cgtcgtgccg cgctccgaga agcgaaaaat cctggcctgc
     1921 acctggactt ccacaaagtt tccgcaccgt gccgattcgg atcatgtcct ggttcgatgt
     1981 ttcgtgggcg gcgccggacg ggaagagctc gcactgcagg acgagggttc tctgatcaag
     2041 atggtctgcg aagatttgaa ggcaatcatg gggatttctg cagaacctgt cttaacgaaa
     2101 gtttaccgtt gggagaaggc gaacccgcag tacaatatcg gtcacctgga gcgggtggct
     2161 gaaatcgaaa aaatgactgc tcaacatccc ggcctctttg tgaccggcgc ggcctatcgg
     2221 ggagtcggcg tagcggactg tattcatcag ggacttcaaa ctgccgagaa ggtattggcc
     2281 cacctcagat ttacaaagca tgtctgattt gaaccgatct tcttctcgat gtataatggt
     2341 ggcgcaccta tggtacggtt atggataaac acgatcacat cccgttgcaa ggcctaagac
     2401 atctcgcaat tcgcgtcacc gatattcaga aagcgcgcga tttctatcaa ggcttgctcg
     2461 gaatgaagac agtctgggag ccggatccgg agaatgttta tctcagctcc ggtcaggaca
     2521 atctcgcgct tcatcagatc ccgaatgccg agcttgcccg ctatcatcag ggcatcggcc
     2581 agtttatgga tcatttcggc tttctagtcg aaaacccgga agaggtcgag gaggtcttca
     2641 aaaagatgga gactgccggg gtcaggatta taaaacccgt aaagcggcac cgcgacggga
     2701 gcatctcgtt ttacatgacc gatccggata agaacgtgat ccagatcctg tacgagccga
     2761 cgatcagcgg gatataaaat aaggggacgc catggaagac tccgatcgca tagctcgttt
     2821 taaaaaagta gccgcgatgg acccaagcag cgaaaccaca ctgtttggtc tcggacaggc
     2881 atatcttgag gaaagcaggt tggaggaagc agcccaggct ttttataagg tgatcgag
//
LOCUS       DATMKH010000694         3184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4148590, whole genome shotgun sequence.
ACCESSION   DATMKH010000694 DATMKH010000000
VERSION     DATMKH010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4148590"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /gene="aroC"
                     /locus_tag="VNV63_08225"
     CDS             complement(<1..622)
                     /gene="aroC"
                     /locus_tag="VNV63_08225"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011971782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HXC62646.1"
                     /translation="MLRYLTAGESHGKLLIGILEGIPAGLSLTSDDINRDLARRQRGY
                     GRGGRMKIEHDTVEIVSGVRKGKTMGSPIGLLIQNRDWENWQERMAVEPGEINEKQKV
                     TKPRPGHADLSGVIKYGHDDVRNSLERASARDTATRVAVGAVAKRLLEEFEMSVFSHV
                     VEIGDVVTSTGTMHPAPTPEKLHKLAEESEVRCADLEASKKMIQKID"
     gene            complement(650..3007)
                     /gene="pilQ"
                     /locus_tag="VNV63_08230"
     CDS             complement(650..3007)
                     /gene="pilQ"
                     /locus_tag="VNV63_08230"
                     /inference="COORDINATES: protein motif:HMM:TIGR02515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HXC62647.1"
                     /translation="MRNKNKMHYSISLVISPLILLGLFACAPKAVPIKPVPQESQEIK
                     DIQVVDLSEKTQIIVETEQPMVYTSFRLTDPLRLVLDLAGTTAGKYHEPIAVNSGAVT
                     YITPVEGVPPSTVVRLEIGLSEAVTTEVVTSGTKLIVDVMKPSSEVSAAPASPETSAP
                     APATPSTEMATEKPKEAAAEPKPALVVTQVKADISNGSVKVIITGDGALKPEVQELSG
                     NRLVVDLPGTTNLMKPNIIQVKHALLKRIRIGQHLKPKKVRVVLDLRRKASYTVEKDN
                     NQVVVALGEASAGAHPSSPTQNETLAEGEPTKPKEQAPKEEAATGQIEKASESSPKLT
                     EPAPVTPSEEKKDAALTPVVVKRDETVSLKRYVGRKISLDFQDAEITNVLRLIADVSG
                     FNIVVGDDVKGKVTVKLINVPWDQALEILLKMNNLGQVREGNIIRIATLANITRQDDE
                     ETKAKEAKSRSEELVTKVIYMNYAKAKELADPLKKNLSPRGDITTDDRTNTMILKDIS
                     RNVVIISDLVKTLDTPTPQVLIEARIIEATTSFARALGVQWGATFTNFNKNGVLGVSG
                     QSFGTGSGGAPTVAPFGNPPPTFAVNLPAGSIAAAGLSSFGGLGFTFGKFTGSPFNLD
                     LRLSAGENQGLSKTISAPKITALDNQEARIEQGESIPFSSTSAQGTQTIFVDANLTLQ
                     VTPHVTSDGSIAMKVKVANNSPDFSTSNPAGPPISKKEANTNILIKDGDTAVIGGIVV
                     NTKADTTQGIPWFEKIPGLGWLFKTNTVSDQTTELLVFLTPRIIK"
     gene            complement(3004..>3184)
                     /locus_tag="VNV63_08235"
     CDS             complement(3004..>3184)
                     /locus_tag="VNV63_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62648.1"
                     /translation="SDGKGYTLKVGTAVGPNDGSVKKISEQNVLIEENYTDIFGERKL
                     RQVVLEIHPQKEGTE"
BASE COUNT          667 a          890 c          750 g          877 t
ORIGIN      
        1 cgtcaatttt ttgaatcatc tttttcgagg cctccagatc cgcacaccgc acttcggact
       61 cctcggccaa tttgtgcagc ttttcaggcg ttggggcagg atgcatcgtt cccgtactcg
      121 tcaccacgtc gccgatctcc acgacatggc tgaagacact catctcaaat tcttcgagaa
      181 gccgtttcgc cacggcccct accgccacac gggttgccgt gtcccgggca ctggcacgct
      241 caagggagtt ccgcacatca tcatggccgt atttgataac ccctgaaaga tccgcatgcc
      301 cgggccgggg cttggtcact ttctgcttct cattgatctc accgggttcc accgccatgc
      361 gttcctgcca attctcccag tcgcggtttt ggattaacaa accaatcgga cttcccatgg
      421 ttttcccctt ccgcacgcct gagacgatct ccaccgtgtc atgctcaatc ttcattcttc
      481 cgccacgacc gtatcccctt tggcgccggg ccaaatctcg attgatgtca tcggatgtca
      541 gacttaagcc ggccggaatc ccctctagaa ttccgatcaa taacttgcca tgggactccc
      601 ctgctgtgag gtatcgaagc atagtttaat ggaccctcat catcagccat catttaatga
      661 tccgtggagt taagaaaacc agcagctcgg tggtttgatc cgacacggtg ttcgttttaa
      721 agagccatcc gagacccggg atcttttcaa accaggggat accttgagtc gtatcggcct
      781 ttgtattcac cacgattcca ccgatgaccg cagtgtcacc atctttgatt aagatattcg
      841 tattcgcctc tttcttcgag atcgggggcc ccgcgggatt actcgttgaa aaatccggcg
      901 agttattagc aaccttcacc ttcatcgcga tgctcccatc cgaagtcaca tggggtgtca
      961 cctgtagcgt aaggttcgca tctacgaaga tcgtttgggt cccttgcgcc gatgtacttg
     1021 aaaatgggat ggactctcct tgctcgatcc tggcctcctg attatccagg gctgtgatct
     1081 ttggagcaga gatcgtcttg cttaagccct ggttttctcc tgctgaaagc cgtagatcca
     1141 ggttgaacgg gctgcctgtg aacttaccaa acgtaaatcc caatcctcca aagctggaca
     1201 gaccggcggc agcgatcgat ccggcgggaa gattgaccgc aaaggttgga ggaggatttc
     1261 cgaagggagc gacagttggg gcgccgcctg aacccgttcc gaaagattgc cctgacaccc
     1321 caagaacacc attcttatta aaatttgtga aggtggcccc ccattgaact cccaacgccc
     1381 ttgcaaaaga ggtggtcgct tcgataattc tggcctcgat caagacctga ggggtcggcg
     1441 tgtcgagtgt cttaaccaag tcgctgatga taacgacatt tcttgatata tcttttaaga
     1501 tcatcgtatt ggtccgatca tccgttgtga tatcaccgcg cgggctcaag tttttcttga
     1561 gaggatctgc gagttccttc gccttggcgt aattcatata gatcacctta gtcacaagtt
     1621 cctctgatct gctcttggct tccttagcct tagtctcctc gtcatcttgt cttgtgatat
     1681 tggcaagggt cgcgatccgg ataatattcc cttcccgcac ctgtccgagg ttattcatct
     1741 tcagaaggat ttccagggcc tgatcccacg gcacattgat gagtttcacc gtcaccttgc
     1801 ccttgacatc atcccccacg acaatattaa aaccgctgac atccgcaatc aggcggagaa
     1861 cattcgtgat ctctgcatcc tgaaaatcca gcgagatttt tcgtccgacg taccgtttta
     1921 aagaaaccgt ttcatctctc ttgacaacaa caggcgtcag tgcggcatcc tttttctctt
     1981 cagaaggcgt taccggtgca ggttccgtga gtttaggaga tgactcgctc gctttttcga
     2041 tctgtcctgt ggcggcttct tcttttgggg cctgctcctt tggctttgtt ggctcacctt
     2101 cggccagcgt ttcattttga gtgggagagg acggatgggc cccagccgag gcttctccaa
     2161 gcgctacaac aacctgatta ttgtccttct caaccgtgta agacgctttc cttctaagat
     2221 cgaggacgac gcgaaccttt ttgggtttga ggtgttgacc aattctgatc cgcttgagta
     2281 aggcatgctt aacctgaatg atattcggct tcatcaagtt ggtcgtcccg gggagatcaa
     2341 ccactaagcg atttccactc agttcttgga cctcaggctt tagagcccca tcgcctgtga
     2401 taatcacttt gacacttccg ttgctgatgt ccgccttgac ttgagtcact accaaagccg
     2461 gctttggttc tgccgctgct tcctttggtt tttccgtagc catctcggta gaaggcgtcg
     2521 caggcgcagg agctgatgtt tccggagaag ccggggcggc tgatacttcc gaacttggct
     2581 tcatgacatc cacaattaat tttgttcccg atgtaaccac ctcggtggtt acggcctcgc
     2641 tcagaccgat ctctaagcgg accaccgtcg aaggcgggac gccctccacg ggcgtgatat
     2701 aggtcaccgc gccgctgttt accgcaatcg gctcatggta tttccctgcg gtcgtccctg
     2761 ccagatcaag aaccagccgc aagggatctg ttaaccgaaa ggaggtgtag accattggct
     2821 gttccgtttc cacaataatc tgcgtctttt ccgataggtc tacgacttga atgtctttga
     2881 tctcctgaga ttcttgggga accggtttga tcggaaccgc cttgggggcg caggcgaaaa
     2941 gacccaaaag gataagcggg cttataacca agctaatgga ataatgcatc ttatttttat
     3001 ttctcattct gtcccctcct tttggggatg gatttccaat accacttgac gtaacttgcg
     3061 ttctccaaag atatccgtat agttctcctc aatcaagaca ttctgctctg aaatcttctt
     3121 tacagaacca tcgttgggtc caaccgctgt tccgactttt aaggtgtacc ctttcccatc
     3181 cgat
//
LOCUS       DATMKH010000695         1390 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1281859, whole genome shotgun sequence.
ACCESSION   DATMKH010000695 DATMKH010000000
VERSION     DATMKH010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1281859"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1256)
                     /locus_tag="VNV63_08240"
     CDS             complement(<1..1256)
                     /locus_tag="VNV63_08240"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HXC62649.1"
                     /translation="MPSQTIPTRSEIAEADTWDLKKLYQTEQDYQQGLEKLKKEYPQY
                     ASYKGKVGQTAQDLLDVLEFDKAVDLLAERLGHYVSLKASEDSSDRENLARKAEFSNL
                     GTKIREVASFITPEIQAIPDERFRILTADPLLKDWRNSLERLRRYRPHTLLEGEEKIL
                     AAGASAIRGHRETFSQLTNVDMIFGVIPDDNGQEIALSQSSYISLLHNRDRKVRRRAF
                     DQFYREFADHRYTLASSLSASVRADVFYARVRNYPSALESALFGDHVPVAVYDNLIAT
                     VRNNLSALHDYYDLRRDLLGLEDIHQYDTFVPLFPEVAKKVGFDEAIELVTASLEPLG
                     DEYVATLRGGLKSRWVDRYETKGKRSGAFSSSSYQNPPFILMNYRADVLSDVFTLAHE
                     AGHSMHTWFAQENQLFQDYGYPIFLA"
BASE COUNT          322 a          357 c          368 g          343 t
ORIGIN      
        1 tcggccagga agataggata gccgtagtct tgaaatagct ggttttcttg ggcgaaccag
       61 gtgtgcatcg aatgacccgc ttcgtgagcc agggtaaaga catccgacag aacatccgct
      121 cgataattca tcaagatgaa gggcgggttc tggtagctcg aagaggagaa agcgccgctg
      181 cgtttaccct tcgtttcata ccgatccacc caacgcgatt tcaggccgcc tcgcagggta
      241 gcgacatatt cgtctcccaa aggctctaat gaggccgtta ccagttcgat ggcttcatcg
      301 aagccgacct ttttggcgac ctccggaaac aacggaacga acgtgtcata ctgatgaata
      361 tcctctaggc ccaggagatc tcttctcagg tcatagtaat cgtgcagcgc cgatagattg
      421 tttctgaccg ttgcgatcag attatcgtat accgctaccg gaacgtgatc tccgaagaga
      481 gcggattcca atgcactcgg gtaattgcgg acccgggcat aaaaaacatc ggctcggact
      541 gaggccgata gggaagaagc cagcgtataa cggtggtccg cgaactctcg gtagaattga
      601 tcgaatgcac gtcggcggac tttccggtct cggttatgca ggagggagat atagctgctt
      661 tgcgagagtg caatctcttg accgttgtcg tccgggatga cgccgaagat catgtcgacg
      721 ttagtgagtt gagagaacgt ctcgcgatgt cctcgaatcg ccgaagcgcc cgcggctaag
      781 attttttctt ctccttccaa aagcgtatgt ggccgataac gcctgaggcg ctccagtgaa
      841 tttcgccaat ctttaagcag aggatcggct gttagaatcc tgaaacgttc atccgggata
      901 gcctgaattt ccggggtaat gaatgaggct acttctctga tctttgtccc caagttcgag
      961 aactccgctt tccgcgcaag attctcacga tccgagctat cctcagacgc tttcaaagac
     1021 acgtagtgcc caagtctctc cgccaaaaga tcaaccgctt tatcgaattc gaggacgtcc
     1081 agtaaatcct gggctgtctg gccgaccttc cctttgtatg aggcgtactg agggtattcc
     1141 tttttcaact tctcgagacc ttgctgataa tcttgctcag tttgatacag cttcttcaga
     1201 tcccaggtgt cggcttccgc aatttcactt ctggtcggga tagtttgaga gggcatgggt
     1261 aaaacgagaa acctgtacct gacacctctc gtgcccgagc gcaagcggag tgacggtagg
     1321 gatatgagct cctgctcatc cgtggttctt tacgttgatc ggacgaacgg atgagccgga
     1381 gctcatccct
//
LOCUS       DATMKH010000696         1034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3510795, whole genome shotgun sequence.
ACCESSION   DATMKH010000696 DATMKH010000000
VERSION     DATMKH010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3510795"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            431..>1034
                     /locus_tag="VNV63_08245"
     CDS             431..>1034
                     /locus_tag="VNV63_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62650.1"
                     /translation="MVFLDETPPWFNESLKEKEEIVYLSPDRETNGIPGVSFLKSSSG
                     SYFRVVYGDGAEFVVDRLGTEIWSIWTGNLTLEDAFVYLLGPIMAFALRLRGLISLHA
                     SAVSIDGNAVAIVGSGGAGKSTTAGSFARMGYPVVSDDVLPLLDHGAFFSVQSGYPCV
                     CLWPKSVESLYGSSDSLPRLTPTWDKRYLALGKNGQSFQRD"
BASE COUNT          220 a          288 c          265 g          261 t
ORIGIN      
        1 taagcctctt gaaccgacat tctactttag aatcggcccc cgccaaactg gaccgctgtt
       61 aagcagatgc cgggaatctt ccgctacaga ggacactggt ggctcagtat gaatatcaag
      121 atgcccggcc cgtcccgaaa gatacgggac ggaccggcgg ctacaaaacc gctctgcgtt
      181 ttagtgttcg gttttgtaga ggccgcccgc atttagggcg ggcagccttc tgtcccgttt
      241 agtaccaaaa tgagtcgcca ccgagaggcc cgccacttgc caccttaaaa ccgatcatgc
      301 atcatgaaga cggtcaagga gaccatcctc accattgcca tttttctatt ccgcatatgg
      361 ccttaatata tgcagcaatc tccccattgc tcggctgaat catgcatcag cgtcggcgcc
      421 cgtggatgtt atggtttttc tcgacgagac tccaccctgg ttcaatgaat cacttaagga
      481 aaaggaagag attgtctatc tcagccccga tcgagaaacg aatggaattc ctggggttag
      541 ctttctaaag tcatcttccg gatcctattt tagagtcgtg tacggcgacg gagcggaatt
      601 cgttgtagac cgtctcggga ccgaaatctg gtcgatctgg accggtaatc tcacactgga
      661 agacgcgttc gtatatctgc ttggaccgat catggctttc gcattgcgcc tgcgcggctt
      721 gatttccctc catgccagtg ccgtctcgat cgacggcaat gccgtcgcca ttgtaggatc
      781 cgggggcgcg ggaaaatcca cgacggcggg gtcctttgcc cggatgggat atcccgtcgt
      841 ctctgatgat gtccttcctc ttctcgatca cggagctttc ttttccgttc aatccggcta
      901 cccctgtgta tgcttgtggc ccaaatcggt ggaatcttta tatggttcct ccgactcact
      961 gccgcgtttg acaccaacat gggacaagcg gtatctggca ttgggaaaga acgggcagtc
     1021 ttttcagcgc gacc
//
LOCUS       DATMKH010000697         1142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2883848, whole genome shotgun sequence.
ACCESSION   DATMKH010000697 DATMKH010000000
VERSION     DATMKH010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2883848"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            25..>1142
                     /locus_tag="VNV63_08250"
     CDS             25..>1142
                     /locus_tag="VNV63_08250"
                     /inference="COORDINATES: protein motif:HMM:NF019368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HXC62651.1"
                     /translation="MAHQEAALIQARALLSNAMHWIADRVASPDPQGLKLVIFNPLSW
                     RRTGPALVEIPMSPQQEPLITAPDGTIVPSQRVQQTSGGTIRYVVAPSNIPSVGYASF
                     KVALMPRKSEETNSEEFANKVYENAFYRLEFAPGGLKSIYDKTLNRELLKTGEFLGGE
                     IFALDSIGCDSSSYTGFGEVAQPSWKTIEKASQYSPHWRLLESGSVRTGWRMEQPFKE
                     ATVRLDMYAYQQTKRLEFNISLLHWSGAIYKEYRMAMPVNIPDGQVSYDAPYAAFEVG
                     KDELQDPAFEGWYSVPSTQIHPRFVQDWISATNGDFGVMLSSQATSWDYLDSKEKKDL
                     FTLLQPILLASRRSCHPQGNFYSPAGDYDFHFALTSFKG"
BASE COUNT          269 a          295 c          301 g          277 t
ORIGIN      
        1 ggtgacatca ctgattctgt atttatggct caccaggagg cggccttgat ccaagcccgg
       61 gcgttgctca gcaacgcaat gcactggatc gcagatcgcg tggcatcacc tgatccgcag
      121 ggcctcaaac tcgtgatatt caatccgctt tcctggagac ggactgggcc ggccttagtc
      181 gaaattccca tgtcgcctca gcaggagcca ctgatcacgg ccccagacgg gaccatcgtt
      241 ccatcgcagc gggttcagca gacttcagga ggaacgatcc ggtatgttgt cgcgcccagc
      301 aatattcctt ccgttgggta tgccagcttc aaagtagcgt tgatgcctag aaagagcgaa
      361 gaaacaaatt cggaagaatt cgcgaacaag gtttacgaga acgcattcta tcggctggaa
      421 ttcgctccgg gcggtctgaa aagtatttat gacaaaacat tgaaccgaga attgcttaag
      481 accggtgaat tcctcggagg agaaatcttc gcacttgatt ccataggatg cgattcaagt
      541 tcctacactg gcttcgggga agttgcgcaa ccttcatgga agacaattga gaaagccagc
      601 cagtactcac cgcactggcg tctcttggaa tctggttctg ttcgcaccgg atggaggatg
      661 gagcagcctt tcaaggaggc cacggttcgc ctcgacatgt acgcctacca acagacgaag
      721 cggttggagt ttaatatctc gctgttacat tggtcggggg cgatctacaa ggaatatcgc
      781 atggccatgc ccgtcaatat tccggacggc caggtgtcat atgacgcgcc gtacgcagcc
      841 tttgaggtgg gcaaagatga gctccaggat cctgcgtttg aaggttggta tagcgttcct
      901 tctacgcaga tccatccccg gtttgtccaa gactggatct cggcgaccaa tggagatttt
      961 ggtgtaatgc tttcctccca ggccacaagc tgggactatc tcgattcgaa agaaaaaaag
     1021 gatctcttta ctttgttgca gccaatcctg cttgcctccc gcaggagttg ccatccccag
     1081 ggaaactttt attctcctgc gggagactat gattttcatt tcgctttgac ctctttcaag
     1141 gg
//
LOCUS       DATMKH010000698         1290 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1911590, whole genome shotgun sequence.
ACCESSION   DATMKH010000698 DATMKH010000000
VERSION     DATMKH010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1290
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1911590"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="VNV63_08255"
     CDS             <1..474
                     /locus_tag="VNV63_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62652.1"
                     /translation="GGFWNPILMSSPLERSKAELESQGYDVWKVGRPATMWAPTLDLY
                     NCIDLVAIRSDRSGVLGIQCCGPDVMPHVHKILEGYTKDKIKKGELVREVIPPNPYLP
                     TWLKAGNLFFIWSWRPFKRHRETRKFLELHQVEFIIESGQVVHREIPNDSETILH"
     gene            529..735
                     /locus_tag="VNV63_08260"
     CDS             529..735
                     /locus_tag="VNV63_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62653.1"
                     /translation="MVAETFLIMALALPAHQNQLDRLRAFLAQTHCHYDVKLGEQPGV
                     GWKMMPFLFEGRHIWAREKLKEAA"
     gene            732..1058
                     /locus_tag="VNV63_08265"
     CDS             732..1058
                     /locus_tag="VNV63_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62654.1"
                     /translation="MKSQLLDRLVEKVLEGESNGSVSEGPFEIGKAYLIRTVTYHQVG
                     ILKDIQGDFLIFKDASWVADSGRFSECLSKGVFNEVEYVGPMLINKTAIVDAFPWENK
                     VPKETK"
     gene            1087..>1290
                     /locus_tag="VNV63_08270"
     CDS             1087..>1290
                     /locus_tag="VNV63_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62655.1"
                     /translation="MERERELEREWELEREREQEQELELEREWEQEQELEREREQERE
                     WEQEQEQELEREQEQEREREREQE"
BASE COUNT          346 a          258 c          416 g          270 t
ORIGIN      
        1 ggcggattct ggaatcccat tctgatgtct tcccctctcg aacggtcaaa agcggaactg
       61 gaaagtcaag gatacgacgt ctggaaagtc gggcgaccgg cgacgatgtg ggctccgaca
      121 ctcgaccttt ataactgtat cgacttggtg gcgatccgct ctgatcgttc gggggtgttg
      181 gggattcaat gttgtgggcc ggacgtgatg ccgcatgtgc ataagattct ggaaggctat
      241 acgaaagaca aaatcaagaa gggagaactg gtacgggaag tgatcccacc aaacccttac
      301 ttgccaacat ggttaaaggc gggaaacctg tttttcatct ggagctggag accgttcaag
      361 cgacatcgag aaacgcgaaa gttcttagag cttcatcaag tagaattcat tatcgaaagc
      421 ggacaagtcg ttcaccggga gatacccaat gactccgaaa caatactgca ttagtgttgt
      481 ggccgtaatt ctctgcgtga tcgcattgcc gttcatgctg gcgctggaat ggttgcggaa
      541 acattcttga tcatggcttt ggctctgcca gcccaccaga accagttgga ccgcctgcgg
      601 gcatttctgg cacagacgca ttgccactat gatgtcaagc tgggcgaaca gccgggggta
      661 ggatggaaga tgatgccatt tctttttgaa ggacgtcaca tctgggcgcg agaaaaacta
      721 aaggaggccg catgaaatca caattgttgg atagactcgt agaaaaggta ttagaaggag
      781 aaagtaatgg atcagtttca gaaggtccgt ttgaaatcgg caaagcctat ctgattcgaa
      841 cggtgacgta tcatcaagtc gggatcttga aagatatcca aggcgatttc ctgattttca
      901 aggatgcttc atgggtagct gactctggac ggttcagcga atgcctgtcg aaaggagtat
      961 tcaacgaagt ggagtatgtt ggcccgatgc tgattaacaa gaccgccatt gttgatgcgt
     1021 ttccatggga aaataaagta ccgaaggaaa caaaatgaac acggctttaa tcggaaatat
     1081 taatacttgg agcgggagcg ggagctggag cgggagtggg agctggagcg ggagcgggag
     1141 caggagcagg agctggagct ggagcgggag tgggagcagg agcaggagct ggagcgggag
     1201 cgggagcagg agcgggagtg ggagcaagag caggagcagg agctggagcg ggagcaggag
     1261 caggagcggg agcgggagcg ggagcaggag
//
LOCUS       DATMKH010000699         1075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2884178, whole genome shotgun sequence.
ACCESSION   DATMKH010000699 DATMKH010000000
VERSION     DATMKH010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2884178"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..>1075
                     /locus_tag="VNV63_08275"
     CDS             2..>1075
                     /locus_tag="VNV63_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXC62656.1"
                     /translation="MKKLSENDPTRGQQIEEYEREIEKLKIQIDSMEEEVRQLRQARS
                     QVEQAYRQNDRLTAALQEAKSQMEALKVEVEKLTAPPSSYGIFSSVNTDGTANVFVSG
                     RKMKVNLHPRIVAKELKKGQELVLNEALNIVETKGFECQGEVVRVKDLLDNLRAVVTL
                     RADEERVAEIADPLLHTKLSVGDHLLFDPRSGYLLEKLPKSEVEELVLEEVPDISYEN
                     IGGLEEQIEQMKDAVELPFLHPDLYAEHKLMPPKGVLLYGPPGCGKTLIAKAVANSIA
                     KKLGDRTGKEIRSFFLHVKGPELLNKYVGESERQIREVFQKAKEKAQEGMPVIVFFDE
                     MDALFRTRGTGISSDVESTIVPQF"
BASE COUNT          284 a          242 c          316 g          233 t
ORIGIN      
        1 catgaaaaaa ctctctgaga atgatcccac ccgtggacaa cagatcgaag agtatgagcg
       61 agagatcgag aaacttaaaa ttcagatcga ttcgatggaa gaggaggtaa ggcagctgcg
      121 ccaggcacgg tctcaggtgg aacaggccta tcgtcagaat gatcgcctca cagcagccct
      181 tcaggaagcg aaaagtcaaa tggaggcctt gaaggttgag gtggagaaac tcaccgcacc
      241 tccatccagt tatggcatct tttccagtgt caacacagat gggactgcga acgtctttgt
      301 gtccggcagg aagatgaagg tgaaccttca tcctcggatc gtagccaaag agctcaagaa
      361 agggcaggag ctggtcctca acgaggcttt aaatatcgtc gagactaagg gttttgaatg
      421 ccagggagaa gtggtgcgcg ttaaagacct gttagacaat cttcgagctg tggtaacact
      481 tcgggcggat gaagagcggg ttgctgaaat cgcggaccct ttgctccata cgaagctgag
      541 cgtgggggat cacctgctct tcgatccccg ttcgggctat cttttggaaa aacttcccaa
      601 gtccgaggtc gaggagttgg tgctcgagga ggttccggat atcagttatg aaaatatcgg
      661 cgggctggaa gagcagatcg agcagatgaa ggacgcggtt gagctgccgt ttctccatcc
      721 ggatctttat gcagagcata agctcatgcc tcccaaaggg gtattgctgt atggtcctcc
      781 tggatgcggc aagaccctta tcgccaaagc cgtcgcgaac tccattgcca agaagctcgg
      841 agatcggaca ggaaaagaga ttcgaagttt cttcctccat gtgaaggggc cggagctctt
      901 gaataaatat gtgggcgaga gcgagcggca gatccgggaa gtatttcaga aagcaaaaga
      961 gaaggcacag gagggaatgc cggtcattgt tttctttgat gaaatggatg cgctcttccg
     1021 tacacggggg accgggatct catccgatgt tgaatcgacg atcgtccctc agttc
//
LOCUS       DATMKH010000700         2673 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4790301, whole genome shotgun sequence.
ACCESSION   DATMKH010000700 DATMKH010000000
VERSION     DATMKH010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4790301"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="VNV63_08280"
     CDS             complement(<1..594)
                     /locus_tag="VNV63_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012900136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HXC62657.1"
                     /translation="MTNTNDSQHRAVLKSIARQAMIQRGLLPDFSPQELAELDKIHGP
                     ATKAQEPTRDLRHLLWCSIDNDDSHDLDQLTVAEVMPDDAARILVAVADVDALVKKDT
                     AIDDHARQNTTSVYTPAMIFPMLPEKLSTDMTSLNYASERYTVVVEMVIAKDGSLQKS
                     DVYAALVRNQAKLAYNSVAAWLENTGPMPKEIATVPGL"
     gene            complement(624..1190)
                     /locus_tag="VNV63_08285"
     CDS             complement(624..1190)
                     /locus_tag="VNV63_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62658.1"
                     /translation="MSRKFGPSVYQHDNKDCWRTWFLEECEAAGLYGVTIGHLKYIKT
                     VPYQSGTIIYLEDAPYFQEHKIHHRICGPYKVVATTHLPQDGKSIHAHDNKVIVYEEH
                     RPESGPWLNRARVRVIEGTPIHIKQMASSDEYFPLRFRIKPLKHYETRLITHEVNCTL
                     CSKRYDSAVFGKKEPLHKKPQAKLIKNK"
     gene            complement(1272..>2673)
                     /locus_tag="VNV63_08290"
     CDS             complement(1272..>2673)
                     /locus_tag="VNV63_08290"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HXC62659.1"
                     /translation="KEEGLTLHRASSDAPQALRPEVRISKGKGLLGWLAQEQRPACFG
                     SLGTTHAYLDYYSRPLPVLSFIAYPILNEGRLEGMLCADSLQAEAFDERAERFLSLMA
                     GEVLHILQDFRELQRIQTKTEEFSGLLSVSKALSSKLDLNHRLETMADLIKDFVSYDQ
                     CLVMLVDPGERRATIKVVRGIEGKDLRDTSFGLSDGLLSLIVKNRHELLFSGLESESR
                     HDRVFPEASRIRIKAKSFLGIPMIVHDRVIGLFILLAQQENAFTGYNKHMLTIVCNQA
                     AQAIADAQLHAEVEQMAVTDGLTSICNHRRFQERLAEEFQRIARHPEPLSLLIADIDH
                     FKRINDAHGHPVGDLILKRIAKILVRLTRSMDIVARYGGEEFVVALFKTESRQTLRLA
                     ERIRKTVESTEFEVQGKTLNVTLSIGVATCPGDARTREELIQFADQALYHAKNTGRNR
                     VAVYHEVREELTRTKA"
BASE COUNT          619 a          673 c          685 g          696 t
ORIGIN      
        1 aaggccggga acagtggcga tctcttttgg catcggcccg gtattttcca gccaggcggc
       61 gacactgtta taggccagct ttgcttgatt gcggaccagc gcggcataga catccgattt
      121 ctgaagcgat ccatctttag ctatgaccat ttcaacgacc acggtatatc gttccgacgc
      181 gtaattgagg gaagtcatat ctgtcgagag tttctcaggc agcattggaa atatcatggc
      241 cggtgtatat accgacgtcg tgttttgtcg tgcatgatcg tcaattgccg tgtccttttt
      301 gacaagagca tcgacatcgg caacggccac caggattctc gcggcgtcat cgggcataac
      361 ttccgcaact gtgagctggt ccagatcgtg tgaatcatcg ttgtctatgg agcaccagag
      421 aagatgccga aggtcgcgcg ttggctcttg agcttttgtc gcaggcccat gaattttgtc
      481 taactcggcg agctcttgag gagaaaaatc cggaagcaat cctcgttgga tcatcgcttg
      541 acgggcaatg ctttttaaaa cagcgcgatg ctggctgtca tttgtattcg tcattctaag
      601 cgaacaccga tgctatagtt tatctattta ttcttaatta atttagcttg gggctttttg
      661 tgaaggggtt ctttcttacc aaaaacagcg ctatcatacc tcttggagca taaagtgcaa
      721 tttacttcat gagtgattaa tcgggtttca taatgtttga gcggttttat tctaaagcgt
      781 agcggaaaat attcatcact actggccatc tgcttaatgt gaatgggagt accttcgatt
      841 accctgaccc ttgcgcggtt taaccaaggt cccgattcag gcctatgttc ttcatacacg
      901 ataactttgt tatcgtgagc gtgaatactt ttcccgtctt gaggtaaatg cgtggttgcc
      961 accactttgt atggtccgca aatacgatgg tgtattttat gttcctgaaa atagggggca
     1021 tcctcaagat atataattgt tccggattgg tatggaacgg tttttatata ctttaaatgt
     1081 cctattgtta caccataaag ccctgccgcc tcgcattcct ccaaaaacca tgtgcgccag
     1141 cagtctttat tatcatgctg atatactgaa gggccaaatt tcctggacat ctttaatcct
     1201 caagaagaat cgtatgtttt tatagaaaaa cagctccgga tttttaggat caagctagac
     1261 cagagctcga ttcatgcttt tgtccgagtg agttcttcgc ggacttcgtg atagaccgcg
     1321 acgcggttgc ggccggtgtt cttggcgtga taaagcgcct ggtcggcaaa ctgaatcagc
     1381 tcttcccggg tacgggcatc ccctggacag gtcgccacac cgatgctcaa ggtgacattc
     1441 aaggtcttgc cttgaacctc aaactccgtc gactcaaccg tctttcggat gcgttccgca
     1501 agccgtaagg tttgccgcga ttcggtcttg aaaagtgcga ccacaaactc ctcgccgccg
     1561 tatcgggcca caatgtccat agaccgggtc aaccggacca ggatcttggc gatgcgtttc
     1621 aagatgagat cgcccacagg atgcccatgg gcgtcgttga ttcttttgaa atggtcaatg
     1681 tcggcgatta agagcgagag cggttccgga tgacgggcaa tacgctggaa ctcttcggcc
     1741 agccgctcct gaaatcggcg gtgattgcag atgctcgtaa gcccgtccgt tacggccatc
     1801 tgctccacct cggcatggag ttgcgcatct gcgatcgcct gagcggcctg gttgcagacg
     1861 atggtcagca tatgcttgtt gtagccggtg aaggcatttt cctgttgagc cagtaggatg
     1921 aagagtccga tcacacgatc atgaacaatc attgggatac ccaaaaaaga tttcgccttg
     1981 atccggatac ggctggcttc cggaaagacc cggtcatggc ggctctcgct ctccagaccg
     2041 gagaagagaa gctcatggcg gttcttcacg atcagagaga gcagaccatc ggagagcccg
     2101 aaagaggtgt cgcgaaggtc ttttccctcg atccctcgaa caaccttgat agtggcccgt
     2161 cgttccccgg gatcaaccag catcacaagg cattggtcat acgacacaaa atccttgatg
     2221 agatccgcca tcgtctccag gcgatgattg agatccaact ttgaactcaa cgccttgctc
     2281 actgagagca gtccggagaa ttcttcggtc ttggtctgaa tgcgttgaag ttcacgaaaa
     2341 tcctggagga tgtgcaagac ctcgccggcc atcaggctca aaaatcgctc ggccctttca
     2401 tcgaaggcct cggcctgaag gctgtcagcg cagagcatac cttccaggcg gccttcgttt
     2461 aagatcggat aagcgataaa ggacaggacc gggagcggtc tggaataata gtcgagatag
     2521 gcatgcgtcg tgccgagact tccaaaacag gcaggccgtt gctcttgagc caaccagccc
     2581 aggagccctt tgcctttcga gatccgaacc tccggccgta gcgcttgagg tgcgtcgctc
     2641 gatgcacgat gcagggtcaa accctcctct ttt
//
LOCUS       DATMKH010000701         1650 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4157841, whole genome shotgun sequence.
ACCESSION   DATMKH010000701 DATMKH010000000
VERSION     DATMKH010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4157841"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1650
                     /locus_tag="VNV63_08295"
     CDS             <1..>1650
                     /locus_tag="VNV63_08295"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HXC62660.1"
                     /translation="VKVDRDERPDLDSRYQMAVSAITGQGGWPLTAFLTPEGQVFFGG
                     TYFPPDNKYGRPGFKSVLSQVRDYYHANLDKVRSSAEGLYRELARHAVETKPGILSYD
                     LITHTIEHISKNFDFLNGGFGAAPKFPHPGALELLLREAFYTGKQDLAAIVARTLAKM
                     GKGGIYDQIGGGFHRYSTDERWIVPHFEKMTYDNSELLKNYLHCYQATEKGFFKEIAL
                     GIIEYVQTVLSDPAQGGFYASQDADISMHDDGDYFTWTVEETEAALSPDEFKVLQLYY
                     NIGPQGEMHANPKKNVLFVDMEPDAIAMKLNLPQSKILNLISTGKQHLLEARMKRPTP
                     FVDKTVYLNWNGMMIAAFLEAYKVLGLAACKELALKTLDLLIAQAYHTEQGFAHSLVD
                     GEPRHFGLLDDQVQMGVALLEAHEVTAEEKYLEKAEAIVKLLLDKFWDDQAGGFFDRA
                     KDNVPSVLGAAHPQKPIQDAPTPSANGVAVMLLDRLGYLTSNLLYRNRAEEILKAFAE
                     TCRDWGLFAATYFQALSYHLQHPAQAVVVGKRNDPATQRFFS"
BASE COUNT          406 a          416 c          451 g          377 t
ORIGIN      
        1 ggtcaaggtg gatcgggacg aacgccccga tcttgacagc cgatatcaaa tggcggtcag
       61 cgcaatcaca ggacagggag gctggccgct caccgcattt cttacgccgg agggtcaggt
      121 gttcttcggt ggaacctatt tcccaccgga caataaatac ggccggccgg gttttaagtc
      181 ggtcttgagc caggtcaggg actactacca tgcaaatctt gacaaggtcc ggtccagcgc
      241 agagggactg tatcgggagc tcgcccggca cgcggtggag accaaacccg gtattttgtc
      301 atacgatttg atcacccata cgatcgagca tatcagcaaa aactttgatt tcttaaacgg
      361 cggttttggt gcagccccca agttccctca ccccggagcc ttggagcttc tcctccgaga
      421 agccttctac accgggaaac aggatctcgc tgcgatcgtg gcacggacat tggcgaaaat
      481 ggggaagggc gggatctacg atcaaatcgg cggcggcttt catcgttatt caacggatga
      541 acgctggatc gttcctcatt ttgagaagat gacctatgac aattcagagc tcttaaaaaa
      601 ctatctccac tgctaccagg ccactgagaa ggggtttttt aaagagatcg cgctcgggat
      661 catcgagtat gtgcaaaccg tgttgtcgga tcccgctcaa ggcggcttct atgcaagtca
      721 ggacgcggac atcagcatgc atgacgatgg ggattatttc acctggacgg ttgaggagac
      781 cgaggcggcg ctctctccgg atgaattcaa ggtgctgcag ctctattaca acataggtcc
      841 tcaaggagag atgcacgcca acccaaaaaa gaatgtcctg tttgtagata tggagccaga
      901 tgctatcgcg atgaaattga acctgccgca atccaagata cttaatctga tcagcaccgg
      961 aaaacagcat cttttagaag ctcggatgaa acggccgact ccttttgtgg acaagacggt
     1021 ctatcttaac tggaacggga tgatgatcgc ggcttttctg gaggcctaca aggttttagg
     1081 actggccgct tgtaaagagc ttgcgctcaa aaccctggac ttgctcatcg cccaggctta
     1141 tcatacagaa caaggatttg cccatagtct cgtggacgga gaaccgaggc attttggcct
     1201 gctggatgat caggtgcaga tgggagtggc gttgcttgag gcgcacgagg tcacggctga
     1261 agaaaagtat ctggaaaagg ccgaggcgat tgtaaagctc ctgctggata aattctggga
     1321 tgatcaggcg ggaggattct tcgatcgggc caaggacaac gttccttccg ttcttggagc
     1381 tgcccatccc cagaagccga tacaagatgc ccccacacca tcagcaaacg gcgtggcggt
     1441 gatgctttta gaccggctgg ggtatcttac ctcgaacctt ctctatcgaa accgggcgga
     1501 ggaaatatta aaagcatttg ccgaaacctg ccgagactgg ggactttttg ccgcgactta
     1561 ctttcaggca ttgagttatc acctccagca tcccgctcaa gcggtggtcg ttggaaaacg
     1621 caatgatcct gctacccaac gttttttttc
//
LOCUS       DATMKH010000702         1270 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3837244, whole genome shotgun sequence.
ACCESSION   DATMKH010000702 DATMKH010000000
VERSION     DATMKH010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3837244"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="VNV63_08300"
     CDS             complement(<1..477)
                     /locus_tag="VNV63_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004235603.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HXC62661.1"
                     /translation="MVTLAAGLDWSIFFRPAFDGSGTYFRFLLVGFEWTVLLSILAWI
                     LAFVVGSIIGVLRTFPGKFLPTLGAVYVEIFRNVPLLVQLFLWYFVFPEIIPGLGTWF
                     KQGLSPLLQQLITGVLCLGLFTAARVAEQVRSGINSLGSGQRAAGLALGLNLLQVYR"
     gene            complement(497..>1270)
                     /locus_tag="VNV63_08305"
     CDS             complement(497..>1270)
                     /locus_tag="VNV63_08305"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein"
                     /protein_id="HXC62662.1"
                     /translation="HRESSIPFSYYDDQNAVVGYSQDLVMLVVDAVKKKLNLPNLQVK
                     LIPVTSQNRIPLVQNGTIDIESGSTTNNLERQQQAAFSDSMFIVGTRLLVKKDSGIQD
                     FPDLKGKNVVVTAGTTSERLIRALNENQGMKMNIISAKDHGESFLTLQSGRAVAFMLD
                     DALLAGERAKARKPADWVIVGTPQSKEAYGMMMGKGDAQFKALVDETIAKAETSGDAA
                     KLYQKWFQSPIPPKNLNLELPLSDEMKALFKSPNDKAFQ"
BASE COUNT          329 a          348 c          303 g          290 t
ORIGIN      
        1 tcgatagacc tgcagcaaat tgagacccag cgctaaccca gctgctcgct ggccgctccc
       61 aagagaattg atcccagagc gcacctgttc cgcgacgcga gccgccgtaa agaggccgag
      121 acataacact ccggtgatca attgctggag taacgggctc agaccttgtt taaaccatgt
      181 tccaaggccc ggaatgatct ctggaaagac aaaataccag aggaaaagct gcaccagcag
      241 cggaacattc cgaaagatct cgacgtaaac cgcaccgagg gtgggaagga attttccagg
      301 gaaagtccga agtacaccga tgatcgatcc caccacaaag gccagaatcc aagcaaggat
      361 cgacagcaga acagtccatt caaaaccaac cagcaggaaa cgaaaatagg taccgctacc
      421 gtcaaaagcc ggcctaaaaa aaatgctcca atccagtccg gcagcgagtg tgaccatcga
      481 atcccgactc tgatctttac tggaaggctt tgtcgttcgg gctcttgaac agtgctttca
      541 tctcatcgct gagaggaagt tcaagattca agttctttgg tggaatgggt gattgaaacc
      601 atttctgata cagtttcgcc gcatctcccg aagtctcggc tttcgcgatg gtttcatcga
      661 ccagcgcctt gaattgggca tctccttttc ccatcatcat cccgtaggcc tccttggatt
      721 gtggggttcc aacaatcacc caatctgccg gcttcctcgc tttcgcacgc tccccggcca
      781 agagggcgtc atccagcatg aaggccaccg cacggccact ctgcaaagta aggaaagatt
      841 caccgtgatc tttggcacta ataatattca tcttcatccc ctgattctcg ttcagtgccc
      901 gaattaagcg ctccgaagtc gtcccggccg taacgacaac gtttttgccc ttcagatccg
      961 gaaaatcctg aattcccgag tcctttttta ccagtaatcg cgtgccgacg ataaacatgc
     1021 tgtcggagaa agcggcttgt tgttggcgct caagattatt ggtagtcgat ccggattcaa
     1081 tgtcgatggt cccgttttgt accagcggaa ttcggttttg tgaagtgacc gggatcagtt
     1141 tgacctgcag attcggcaga ttcagcttct tcttaacggc atcaaccacc agcatcacaa
     1201 ggtcttgaga gtaaccgact accgcgttct ggtcgtcata ataggaaaac ggaatcgacg
     1261 attcacggtg
//
LOCUS       DATMKH010000703         2031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4793549, whole genome shotgun sequence.
ACCESSION   DATMKH010000703 DATMKH010000000
VERSION     DATMKH010000703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4793549"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1195
                     /locus_tag="VNV63_08310"
     CDS             <1..1195
                     /locus_tag="VNV63_08310"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXC62663.1"
                     /translation="KESIEKGREVFMKKGKCVECHGVDARGDGNLSQKNEFGDPIFPA
                     DLHKCWNLRGNRRDPYNPRNIFREVSTGLNGTPMPSFADILTPEDRWNVANFVIAQCP
                     KRPIDPLTNKPTISFVVASRFIEGELPTDMNDPQWQQREPSYIGLGSQIIHKPRHFTR
                     TVDEIWVRSLYNKKEISFLFEWDDRTKSLASGPPPAEIKEVPGTVMAARVVPEVYNDA
                     IAIEFPTHWQDLVVPEKPRFIFGGDPTKLTRGEKSTDIKKSVDLWKWEADGSLYDFTG
                     QGWNDITPRPESAKRLKAEGATFANGKWSLILKRSLTTEDKDNDVQFDATGKYIPIAF
                     FAWDGSNGETDIKMGLSTWSYMVLEPPIPKTAYVYPPLAVVLVVGIEFWIYTKFRNNK
                     NGRKK"
     gene            1345..>2031
                     /locus_tag="VNV63_08315"
     CDS             1345..>2031
                     /locus_tag="VNV63_08315"
                     /inference="COORDINATES: protein motif:HMM:NF015418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HXC62664.1"
                     /translation="MIKQTSEKEMASVHQALEEVLGRSRRLEAEQILIHAAIGRVLAE
                     DLVAVTDMPSWDNSAMDGYAVRYEDLQGASRANPAVLRVLDVQPAGKISKKRVETGTA
                     LKVMTGAALPQGADAVVKVEDTLSDHDQAKFFRPVNRMENVRPRGEELKKGEWVLSEG
                     TLLRSAEIGMIAALGNSMVRVYHRPAVAILATGNELIEPGLRLSEGQIFNSNSHSLAA
                     QVVEAGAIPRL"
BASE COUNT          508 a          485 c          579 g          459 t
ORIGIN      
        1 gaaagaaagc atcgagaagg gccgtgaagt ttttatgaag aagggaaagt gtgtggagtg
       61 ccatggggtg gatgctcgag gggatggaaa cctgagccag aagaatgaat ttggtgaccc
      121 gatctttccg gccgatcttc acaaatgctg gaacctccgt ggaaaccgtc gagatccgta
      181 taacccccgt aacatcttcc gtgaggtctc gaccggtctc aatgggaccc cgatgccgtc
      241 tttcgcggat atacttacac cggaagaccg ctggaacgtg gccaactttg tgatcgctca
      301 atgccccaaa cggccgatcg atcctctcac gaataagcca accattagct ttgtggtcgc
      361 atccaggttc atcgagggag aacttccaac cgatatgaat gatccgcagt ggcaacagcg
      421 ggagcccagc tacatcggtc tgggaagcca aatcatccac aagccccgtc actttacccg
      481 gacagttgat gagatctggg ttcggtcgct ctacaacaag aaagaaatct ccttcctctt
      541 cgagtgggac gatcggacca agagcctggc ttccggtcca ccacctgccg aaattaaaga
      601 agtcccgggt actgtcatgg ccgcacgcgt ggtaccggag gtgtacaatg atgcgattgc
      661 gatcgagttc ccaacccact ggcaggatct ggtggtcccg gagaagcccc gctttatatt
      721 cggcggggat cccaccaagc tcacacgagg tgagaagtct accgatatta agaaatcggt
      781 ggatctctgg aaatgggaag cggatggcag cttgtatgat tttacaggac aagggtggaa
      841 tgatatcact ccgaggcctg aaagcgctaa gcgcttgaaa gccgaagggg caacttttgc
      901 aaacggcaag tggtctttga tcctgaaacg gtctctgacc acggaggaca aggataatga
      961 tgtccagttt gatgccacgg ggaagtacat cccgattgcc ttctttgctt gggatggcag
     1021 caacggagag acggatatta agatgggtct ctctacttgg tcctacatgg tactggagcc
     1081 gccaatcccg aagaccgctt atgtctatcc tcctctcgcg gtggttttgg tagtgggtat
     1141 tgagttctgg atctatacga agtttagaaa taataagaac ggccgaaaga agtaaataaa
     1201 cgccgggtta aatcgaaaag attcgttccg gaaaggtgag tatagtatat tttgctatac
     1261 tcaccttttt attttctgcc tcggggttgt tcaggtttac actttacact gcacgcaaag
     1321 atggtataca atacagccgc ttttatgatc aaacagacaa gtgaaaagga aatggcgtcg
     1381 gttcatcagg cgctagagga agtgcttggc cgatcccggc ggctcgaggc agagcagatt
     1441 ctcattcatg cggctatcgg ccgggtcctt gcagaagacc tggttgccgt gacagatatg
     1501 ccgtcctggg ataattcggc gatggacggc tatgcggttc gatatgaaga tcttcaaggg
     1561 gcttcacggg caaaccccgc cgttcttcga gtcctggatg tccagcctgc cggaaaaatc
     1621 tctaagaaac gggtcgaaac cggtacagcg ctcaaggtca tgacgggggc tgccctgccg
     1681 cagggtgccg atgcggtggt caaggtagaa gacacgctct cggatcatga ccaggcaaaa
     1741 tttttcaggc cggtgaatcg aatggagaac gtgaggcctc gcggagaaga actcaagaag
     1801 ggtgaatggg tattatccga aggaacgctt ctgcgttcgg cagagatcgg gatgatcgcg
     1861 gccctgggca attcaatggt tcgtgtctat catcggccgg ctgttgcgat tctggcaacc
     1921 ggaaatgagc tcattgaacc cggcttgaga ctatccgagg gccagatttt taacagtaac
     1981 agccattcat tagcggcaca agtggtcgag gcgggcgcga ttcctcgttt g
//
LOCUS       DATMKH010000704         2851 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4159900, whole genome shotgun sequence.
ACCESSION   DATMKH010000704 DATMKH010000000
VERSION     DATMKH010000704.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4159900"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..134)
                     /locus_tag="VNV63_08320"
     CDS             complement(<1..134)
                     /locus_tag="VNV63_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015812979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HXC62665.1"
                     /translation="MLSKKAKYALKALLVLAEEYNQGPVLISDLAEREGIPKKFLEQI
                     L"
     gene            complement(257..1000)
                     /locus_tag="VNV63_08325"
     CDS             complement(257..1000)
                     /locus_tag="VNV63_08325"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXC62666.1"
                     /translation="MNGTTAPRQATTTEEILIIEDDLDTVKLLTQELTEHRYGVRAAR
                     DGQIGLAEAQRQPPSLIILDLLLPGLSGWEVCRSLKSYPTTKSIPLLILTALGQEENR
                     VRGLELGADDFLAKPFSLKELVARIRALLRRSRISTEDKTTGHLKIGPLSIDMERHEV
                     RMVGRLLQLTRTEFALLKCLAQHPGRVFTRDELITALWGEDRFVEEHNLDVHIHSLRQ
                     QLEPESAHPMFLLTVRGVGYKLQITDKDQ"
     gene            complement(1097..2488)
                     /locus_tag="VNV63_08330"
     CDS             complement(1097..2488)
                     /locus_tag="VNV63_08330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXC62667.1"
                     /translation="MIKFTIQWKFLAASMFIAIASLAVAGFLTLDAQQEHLRRLLFIT
                     LASGLGLVLALFYLFFRRLSISLHEMTSVAKDLAKGRLEKRVRITAHDELAELGGVLN
                     QMASALEAEMWEITEDRARLAAILSGMVEGVMVLDRQGRILMINIAMERMFRLKGDLI
                     VGKTFIEVLRHHQLNEFIQTTLKTPANRSVEIMIQTPEERVFTVQASTAAETQESSPV
                     AVFVFHDVTELKRLEHVRKDFVANVSHELRTPLTSIKGYLEALVDGAKDDPQKCLQFL
                     EVAQQHADQLNNLITDLLQLSQIESGRYEWKRERINPGNLIGKAIELMRPMAEKKHQS
                     LTISSGSSLPPITGDTDRLTQVLINLLENAVKYTPEGGKIEVEARRNGDSMEIIVNDT
                     GLGIPKKDLPRIFERFYRADRARSRELGGTGLGLSIVKHIVEAHGGGVQAESEPGKGS
                     KFTIVLPCNVPNL"
     gene            complement(2506..>2851)
                     /locus_tag="VNV63_08335"
     CDS             complement(2506..>2851)
                     /locus_tag="VNV63_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXC62668.1"
                     /translation="VKALLRRTEPSHSPKAQTYVYSDLHLDAVKHEVLVKSKEVSLTA
                     KEFGLLEQLLQNKGHILTRDVLLDRVWGYDADVTTRTVDVHVRRLREKIPLLADAILT
                     VKSYGYKLREED"
BASE COUNT          647 a          772 c          714 g          718 t
ORIGIN      
        1 aggatctgct ccaaaaattt tttggggatg ccctctcttt ctgccagatc cgatatcaat
       61 accgggccct ggttgtactc ctcggcaagc acgagcagcg ccttgagagc gtattttgct
      121 ttcttagaga gcatctctat actctacaat ctctataaac tttgtagagt attgtctatg
      181 ttgatatcgt ttgtcaagtc ttttttttaa ccaaccccgc cgcccttcat cgtcatccat
      241 ttcttaattg cttcggtcat tgatccttat ctgtgatttg aagcttgtat ccaacacctc
      301 gtacggtcaa cagaaacatc ggatgggcgg attccggctc cagctgttgt ctcaatgaat
      361 ggatgtggac atcgaggtta tgctcctcta caaaccgatc ttctccccat agggctgtaa
      421 tcagctcgtc gcgggtaaag acccggcccg gatgctgagc aagacatttc agaagagcaa
      481 actcagtccg agtgagttgc aggagccgcc ccaccatccg aacttcgtgg cgttccatat
      541 caatggacaa tggaccgatc ttaagatgac ctgtagtttt atcttccgta cttatccggc
      601 tgcgccgcaa caacgcccgg atgcgggcga ccagctcctt gagactgaaa ggcttggcaa
      661 gaaaatcatc tgcccctaat tccagtcccc tcacccggtt ctcttcctgt ccgagtgcgg
      721 tgagaatcag aagaggaatg ctctttgttg ttgggtaact cttgagtgaa cggcagactt
      781 cccaaccgga cagcccgggc agcagcagat ccagaatgat taagctaggc ggctgccgct
      841 gggcttcggc caaaccgatt tggccatcgc gtgcggcccg cacaccatag cggtgctctg
      901 tgagttcctg cgtaaggagt ttgacggtgt ccaaatcatc ctcgatgatg aggatttctt
      961 cggtcgtagt ggcctgccgg ggtgcagttg tgccattcat cttgaaggta agactactaa
     1021 acggacgtta agggaagatc aagagcaggt taattctaga taaacagaaa gccggaagca
     1081 gaatggcgtt taagaatcac aaattgggga cgttgcaagg aagaacaatg gtaaacttcg
     1141 atccttttcc cggttcgctc tccgcttgca caccgcctcc gtgcgcttcc acgatgtgct
     1201 tgacgatgga aaggccaaga ccggtaccgc caagttctcg ggaacgagcc cggtccgccc
     1261 gatagaaacg ctcaaaaatc cggggaagat cttttttagg aattccaaga cctgtatcgt
     1321 ttacgataat ctccatcgaa tcaccattcc ggcgcgcctc aacttcgatc tttcctccct
     1381 ccggtgtata cttaacggca ttctccagca gattgatcaa tacctgtgtg agcctgtcgg
     1441 tatcaccggt gatcggtgga agcgagctgc cggaagagat cgtcaatgat tgatgtttct
     1501 tttcagccat cggcctcatc aattcaatcg cctttccgat aagatttccg gggttgatcc
     1561 gctcacgttt ccactcgtac cggccggact cgatctggga gagttgaaga agatccgtaa
     1621 tcaggttgtt caactggtcg gcgtgctgtt gtgccacctc cagaaattgc aggcatttct
     1681 gagggtcgtc tttggcgccg tccacaagcg cctcgagata acctttgatg gatgtgagcg
     1741 gcgtccgcaa ctcatgcgaa acattcgcta caaaatcctt tcggacatgc tccaaacgtt
     1801 taagttcggt cacatcgtga aagacgaaca cggctaccgg gctgctctcc tgcgtttccg
     1861 cagcggtcga agcctggacc gtgaatacac gctcttcagg agtttgtatc ataatctcga
     1921 ctgaacgatt cgcgggtgtc ttcagcgtcg tttgaataaa ttcgttcagc tggtggtgtc
     1981 ttaagacttc gatgaaggtc ttcccgacga tgaggtcgcc tttgagtctg aacatccgtt
     2041 ccattgcgat attgatcatg aggatccgcc cctgtctgtc caaaaccatc actccttcca
     2101 ccatgccgct aagtatggcc gcaagccttg cacgatcctc cgtgatttcc cacatctcgg
     2161 cctccagcgc cgaggccatc tggtttaaga cccctcccag ctccgccagc tcatcgtgtg
     2221 ccgtaattcg cacccgtttt tcaaggcgcc ctttggcaag atccttcgca acggaggtca
     2281 tctcatgtaa tgagatggac agacgtctaa aaaacagata gaaaagcgcc aagaccaagc
     2341 caaggcccga ggccagcgtg atgaataata accgtcgaag atgttcctgc tgggcatcca
     2401 gggtcaggaa acccgcaact gccagactcg cgatcgcgat gaacatcgag gccgcgagga
     2461 acttccattg gatcgtgaac tttatcattt gaaccttagg cggactcagt cctcttctct
     2521 gagcttgtag ccgtaagact ttacggtgag gatggcatcg gccaggagcg gaattttttc
     2581 ccgaagcctg cggacgtgaa cgtcgacggt acgtgtggtt acatctgcat catatcccca
     2641 cacccgatcc aggagtacat cgcgcgtgag aatgtggcct ttgttttgaa gcagctgttc
     2701 gagcagcccg aactccttgg cggtcagcga cacctctttg ctcttgacca ggacttcgtg
     2761 tttgacagca tccagatgaa ggtcgctgta aacataggtc tgggccttgg gactatggga
     2821 cggctccgtc ctccggagca gtgccttgac g
//
LOCUS       DATMKH010000705         1628 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4159955, whole genome shotgun sequence.
ACCESSION   DATMKH010000705 DATMKH010000000
VERSION     DATMKH010000705.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4159955"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="VNV63_08340"
     CDS             complement(<1..627)
                     /locus_tag="VNV63_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXC62669.1"
                     /translation="MKILIHGGRVVDPASGMNEPADILIEDGKIVDIKKPGNRKRTED
                     EAKIQRIDATGKLIFPGLIDVHTHVREPGFEYKETIRTATEAAVAGGFTTICAMPNSQ
                     PVNDNQSVTRFILNRAQSEGLVQVLPIGAITKGSQGEELAEIGELKEAGCVAITDDGH
                     PVEKSSVMRLAMEYAKTFDLPVIDHCEDPLLSEGGVMNEGTVATELGLP"
     gene            complement(624..1559)
                     /locus_tag="VNV63_08345"
     CDS             complement(624..1559)
                     /locus_tag="VNV63_08345"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HXC62670.1"
                     /translation="MLKRKDLLSITDLTPDEITVILDTAVSFKEVMARDIKKVPTLRG
                     KTVINLFVEPSTRTRTSFELAAKRLSADIVNISSSTSSLTKGEGLLDMARNLEAMRAD
                     LIVLRHPSAGAPHLLARTLQCGVINAGDGAHEHPTQALLDVFTIRERKKTISGLRVLL
                     VGDILHSRVARSDLHALKKMGAQVTLVGPGTMIPRGIEPWGVQVAYNFDEALDKADQK
                     PDVIIMLRLQLERQEKGFIPSLREYSKLYGLTMARLRRAAPDALVMHPGPINRGIEIA
                     PDVADSLSSVILDQVTNGVAVRMALLYLLMGGKAE"
BASE COUNT          350 a          494 c          396 g          388 t
ORIGIN      
        1 gggcaacccc agctctgtgg ccaccgtccc ttcattcatc acaccgccct cggataataa
       61 cgggtcttca caatgatcga tcaccggcag atcaaaggtc ttcgcatatt ccatcgcgag
      121 acgcatcacg gagctttttt caaccggatg cccgtcatcc gtgatagcga cgcaaccggc
      181 ctctttaagc tcgccgatct cggccagctc ttctccttga gaacccttag tgatcgcccc
      241 gatcggcaag acctgaacaa ggccttccga ttgagcccgg ttcagtataa aacgcgtaac
      301 cgactggtta tcattcaccg gctggctatt gggcatcgca cagatcgtcg tgaaaccacc
      361 ggccactgcg gcctcggtcg cggtccgaat cgtttctttg tattcaaagc cgggttctct
      421 cacatgcgtg tggacgtcaa ttaatccggg aaagatcagc tttccggtcg catcaattcg
      481 ctgaatcttc gcttcgtcct ccgtcctctt acgatttccc ggctttttta tatcaacaat
      541 ctttccatct tcaatcagga tatccgcagg ctcattcatg ccgcttgcgg gatcaaccac
      601 tctcccgccg tgtataagga ttttcattcc gccttgcctc ccatcagcaa atagagcagc
      661 gccatacgca ccgccacgcc attggttacc tgatccagta tcacggatga gaggctgtcc
      721 gccacatccg gcgcgatctc aatcccgcga ttgatcggtc caggatgcat gaccaacgcg
      781 tccggggcgg cacgacgaag acgggccatc gtcaatccgt acagtttcga atattcccga
      841 aggcttggga tgaagccctt ttcctgacgc tccaactgga gcctcagcat gatgataaca
      901 tccggttttt gatctgcctt atccaaagct tcatcaaaat tgtatgcgac ctggacgccc
      961 cacggctcga ttcctctggg gatcatcgtg ccgggtccga cgagagtgac ttgagccccc
     1021 atctttttta aggcatgcaa gtccgaccgg gcgacacggc tgtgaagtat gtcccctacc
     1081 agaagcacac gcaaccccga aatcgttttc ttacgctcgc ggattgtgaa gacatccagg
     1141 agcgcctgag tggggtgctc atgtgcgcca tcaccggcgt taataacccc gcattggagc
     1201 gtcctggcaa gaaggtgcgg cgcacccgcg gacggatgcc ggaggacgat gagatcggcg
     1261 cgcatcgctt ccagattcct ggccatatcg agaaggcctt cgcctttggt cagactgctt
     1321 gtggagcttg agatgttgac gatatcggcc gagaggcgct tcgcggccag ctcgaaagaa
     1381 gtccgggtcc gtgtactggg ttccacaaag agattgatca cggtctttcc gcgaagtgtc
     1441 gggaccttct taatgtctct cgccatcacc tccttgaagg atacggccgt atccaaaatc
     1501 acagtgattt catcgggagt gagatccgta atcgaaagaa gatctttacg ttttaacatg
     1561 gaggttctcc aacaccaccc gatcctcctc gccctcttct tctaatagca ccttgaccac
     1621 ttcatccg
//
LOCUS       DATMKH010000706         2310 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1920173, whole genome shotgun sequence.
ACCESSION   DATMKH010000706 DATMKH010000000
VERSION     DATMKH010000706.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1920173"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..585)
                     /locus_tag="VNV63_08350"
     CDS             complement(10..585)
                     /locus_tag="VNV63_08350"
                     /inference="COORDINATES: protein motif:HMM:NF017834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXC62671.1"
                     /translation="MPLEQRINALRPDGVEQACLLASQGFTDAQIAAAFGVREVTVNR
                     WKKENREFEISLKKGKIEADSRVTKGLYQRAIGYDYEEVHTEALKSANNKPAVQRIKK
                     ITRHVPPDVIAQIFWLKNRQPEMWRDKHDVHHQSQVGVQVEIMPPLSRKEKIEEVKEK
                     IRILLESGALQLPVELLGEGPGAKDDEVHPP"
     gene            complement(632..1888)
                     /locus_tag="VNV63_08355"
     CDS             complement(632..1888)
                     /locus_tag="VNV63_08355"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HXC62672.1"
                     /translation="MRIETVPISKLKPSAYNPRKNLTPGDPEYESLKKSIETFGCVEP
                     IIYNRRSGNVVGGHQRLKILKGRGAKQVTVSVVDLDPIKEKALNVALNRIQGEWDFPK
                     LKDLLVELDTGDFNLGITGFSETELQSLIDHEGTPGLTDEDEVPPTPKKAISNSGDLW
                     ILGDHRLLCGDATKEADLAKLMDGHKADLVITDPPYNVNYLSGKQGGIKNDNLEPEQF
                     QKMLALAFTAIDRNLAPGAVQYYFSGWSSLPDFLNALRKVQFNLSAVIVWDKGTPGMG
                     WQDYRYQYELLVMGHKMMHHQYEAIVYGFKKNKTHKFKAGRDETDIWYCRRALTKDYE
                     HPTQKPVEVIQRAVMNSSAMGQVVLDPFAGSGSTIIACERMGRKCFAMELDPKFCDVA
                     RERWAKFTGKKASLARAGKKLKVANV"
BASE COUNT          457 a          647 c          561 g          645 t
ORIGIN      
        1 ggcgtgggcc tatgggggat gtacttcgtc gtccttggct ccagggcctt ccccaagtaa
       61 ttccactggg agttggagcg ccccggattc gaggaggatg cggatctttt ctttgacttc
      121 ttcaatcttt tccttcctgg atagcggtgg cattatctca acctgaacac ccacctggct
      181 ctggtggtgg acatcgtgct tgtcccgcca catttccggc tggcgattct tcagccagaa
      241 gatctgggcg atcacatccg gcggcacatg cctagtgatc ttcttgattc gctggacagc
      301 aggtttgttg ttcgcggact taagcgcctc ggtgtgcact tcctcatagt cgtatccgat
      361 cgctcgttga tacaggccct tcgtcaccct gctgtccgcc tcaatcttcc cctttttaag
      421 ggatatctca aactccctgt tctctttctt ccaccgattg accgtgactt cgcggacgcc
      481 gaacgccgct gcaatctggg catccgtgaa tccctgggat gccaggaggc atgcctgttc
      541 gaccccgtcc ggccggagcg cgttgatcct ttgttctaga ggcaccctgg gccttccgcc
      601 agggcccttt ttcttccccg atccgttttt cctagacatt ggccaccttg agcttctttc
      661 ccgctctggc aaggcttgct ttcttgcctg tgaattttgc ccatcgttcc ctcgcgacgt
      721 cgcagaattt agggtcaagc tccatggcga aacactttcg gcccatccgc tcgcatgcga
      781 tgatggtcga tccgctccca gcaaatgggt caagaactac ttgccccatg gcagacgagt
      841 tcatgacagc tctctggatt acttccactg gcttttgagt aggatgctcg taatccttcg
      901 tgagtgctct ccggcagtac caaatatcag tctcatcgcg accagcctta aacttgtgag
      961 tcttattctt cttaaaccca tagacaatag cttcgtattg atgatgcatc atcttatgcc
     1021 ccattaccaa tagctcatac tggtatcgat agtcctgcca tcccatacct ggagttcctt
     1081 tgtcccacac aattacagca gaaagattga actgcacctt tctgagtgca ttcaggaaat
     1141 caggaagact tgaccatcca gaaaaatagt actggacggc tccgggtgcg aggttgcggt
     1201 cgatcgcggt gaaggccaag gccagcatct tttgaaactg ctctggctca agattatcgt
     1261 tcttgatccc gccctgcttg ccgctcaggt agttgacgtt atacggcggg tccgtgatga
     1321 ccaggtcggc cttgtgacca tccatgagct tggccaggtc agcctccttg gtcgcatccc
     1381 cgcacaagag acggtgatcc ccaagaatcc acagatcccc cgagttgctg atcgccttct
     1441 tcggggtcgg aggcacttca tcctcatctg tgagccctgg tgttccttcg tggtcgatca
     1501 gactctgaag ctcagtttca ctgaaccctg tgatccctag attgaagtct ccggtatcca
     1561 gctccaccag taaatccttg agctttggaa aatcccattc accttggata cggttcaagg
     1621 caacgttcaa ggccttttcc tttatgggat cgaggtccac cacggacacc gtcacttgct
     1681 tggcgccacg gccctttaat atcttaagac gttggtgacc tcctactacg ttgcctgatc
     1741 tcctgttgta aattatcggt tccacgcagc caaaggtctc gatggatttc ttgagggact
     1801 cgtattccgg atcacccggt gtgaggttct ttctgggatt gtaggcgctg ggttttagtt
     1861 ttgatatggg tacagtttct attctcatgt gtctctccaa atttgttatg cagaaggccc
     1921 tggcaaggcc tgcatgatcc atacgcgata gatccaggtc ggcctcaact cttgaatcac
     1981 ctcttgatta attttcaatg atctctcttt gccggcgtgt accacttagc actttcctat
     2041 gcgctaccgt gcattcctga actgcattgc gctctttttt cctcttctct ctctatcaag
     2101 aatttaggac ttgacagatg agagtgtttc agaggcaaag aggttcgact cgagagtttc
     2161 gagtcgagga ggacaagagt cagccacgcc aaaggttttc ccgtgtgagg gagctttgaa
     2221 acgcggcgta atgcaacgcc tcgagctgaa gcaatgcagt ctgggagtcc attccctgac
     2281 tgaaagaata acgaggcgtt gtagagccgg
//
LOCUS       DATMKH010000707         1338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2557992, whole genome shotgun sequence.
ACCESSION   DATMKH010000707 DATMKH010000000
VERSION     DATMKH010000707.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2557992"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /gene="def"
                     /locus_tag="VNV63_08360"
     CDS             complement(<1..477)
                     /gene="def"
                     /locus_tag="VNV63_08360"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HXC62673.1"
                     /translation="MAVLKILQYPHPLLKSRSKQVHTIDGRINRIIQDLLDTLHASPG
                     VALAAPQIGESYRIIVCDVSRKIKEHHHGQIILINPIIQRREGKNIFREGCLSVPDYT
                     GNVSRAERVWVEGLSPQGLRVNLNAAGFEALALQHEMDHLEGILFLDRIASLSTDLF"
     gene            complement(479..1039)
                     /locus_tag="VNV63_08365"
     CDS             complement(479..1039)
                     /locus_tag="VNV63_08365"
                     /inference="COORDINATES: protein motif:HMM:NF025116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HXC62674.1"
                     /translation="MKKPADRFVIITAAGPDRPGIIARITQALFRTGCNIEDTSMTLL
                     RGEFAMMLIVRLPEKPGLAEIKFQLKKVQTALGLSLLLKPLSTREAQRDQRPAGRSFI
                     LSVYGADKPGIVYRVTRLIASYRINITDMNTRVIGLREAPIYVIVLEMEIPKKVKVQT
                     LQTELNKLKKAVHVDITLHPVESAQL"
     gene            complement(1045..>1338)
                     /locus_tag="VNV63_08370"
     CDS             complement(1045..>1338)
                     /locus_tag="VNV63_08370"
                     /inference="COORDINATES: protein motif:HMM:NF039902.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HXC62675.1"
                     /translation="EFDPKAKEKFLKPSVRPLFEKLTERLENAATFDSAAIEAIFKEL
                     IAAAGIKLGDLAQPVRIALTGRTVSPGIYEVMELLGKERTVARLKNAVSLMGT"
BASE COUNT          286 a          349 c          372 g          331 t
ORIGIN      
        1 gaacagatcc gtactcaggg aagcaatccg gtccaagaac agtatgccct ctaagtggtc
       61 catctcatgc tggagggcca gggcctcgaa gcccgccgcg ttcagattaa cgcgcagtcc
      121 ttgggggctc agcccttcga cccagacacg ctctgcccga ctcacattcc ccgtgtagtc
      181 cgggacgctc aagcagcctt ctctaaaaat attctttcct tctctgcgtt gaatgatggg
      241 attgatcagg atgatctgtc catggtgatg ttcttttatt ttccgcgaga catcgcagac
      301 gataatccga taagactcgc cgatctgcgg tgcggccaag gccaccccgg gggatgcatg
      361 aagagtatcc agaagatcct gtatgatccg attaatccgg ccatcaatag tatgaacctg
      421 cttggaacgg gacttgagaa ggggatgggg gtactgaagg atttttaaaa cggccatatc
      481 aaagttgagc tgactctacc gggtgaagtg tgatgtctac atggaccgct ttctttagct
      541 tgtttagctc ggtctgcagt gtctgaacct tcaccttctt cgggatctcc atctccagga
      601 ctatgacata gatcggtgcc tctcgcaggc cgatcacgcg tgtattcatg tccgtgatgt
      661 tgatgcggta ggacgcgatc agtcgggtaa cacggtaaac gattccgggc ttatcggcgc
      721 catatacaga gagaatgaag gaacgcccgg ccggccgttg atctctctgg gcctcccgtg
      781 tggaaagcgg ttttaaaaga agcgacaggc cgagtgcggt ctgaaccttc ttgagttgga
      841 atttgatctc cgcaaggccc ggcttttcgg ggagccgaac gatgagcatc attgcaaact
      901 ctccccgaag gagtgtcatg ctggtatctt cgatattgca gcccgttcga aaaagagctt
      961 gtgtgattcg cgcgatgatg ccgggccggt caggtcctgc ggcggtgatg atgacaaacc
     1021 ggtcggcggg ttttttcatg cctttcaagt tcccatcaaa gaaaccgcat tttttaggcg
     1081 ggcgaccgtg cgctctttgc cgagaagttc catgacctcg tagattcccg ggcttacggt
     1141 gcggccggtc aacgctatac gcacgggctg cgccagatcg ccgagcttta tgccggcggc
     1201 cgcgatcaat tctttaaaga tagcctcgat cgcggctgaa tcaaacgttg cggcgttttc
     1261 caatcgctcg gtgagtttct cgaacaaagg ccgcactgac ggtttcagga acttctcttt
     1321 tgccttgggg tcgaattc
//
LOCUS       DATMKH010000708         1747 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3842333, whole genome shotgun sequence.
ACCESSION   DATMKH010000708 DATMKH010000000
VERSION     DATMKH010000708.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3842333"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1212
                     /locus_tag="VNV63_08375"
     CDS             <1..1212
                     /locus_tag="VNV63_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018509014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyltransferase domain-containing protein"
                     /protein_id="HXC62676.1"
                     /translation="VYRASGDSYILVEYGPLVLDLNLRFRVHALMQLVQGHRLSGIVD
                     LTPGIRSLQVHFDPKKVPLRELLDVLLTAEKELPLADEMEVPTRIVHLPLSWEDESTL
                     LAIQKYMQSVRADAPWCPSNLEFIRRVNGLRSIAEVKDIVFDASYLVLGLGDVYLGAP
                     VAIPIDPRHRLVTTKYNPARTWTPENAVGIGGAYLCIYGMEGPGGYQFVGRTVQVWNR
                     FHVTKNFEAGKPWLLRFFDQIRFYPVSAEKLLRHREDFLSGKFNLTIEEETFRLRDYH
                     AFLKRIVPEAAAFKADQQAAFEAERQRWASTEFVPADDTSSMALQEEVQLPEGGSFID
                     APVAGNLWKLVTKPGDQVQPDQPVAIIESMKMEMQVCATSLGTVHSVLVKEFSPVSQG
                     QHLFIIESPRL"
     gene            1315..>1747
                     /locus_tag="VNV63_08380"
     CDS             1315..>1747
                     /locus_tag="VNV63_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62677.1"
                     /translation="MSEFPEIQPFGPRPAQHNEDPQNAAPAAHPSEPPTPSPSQAPAS
                     RVNITVVVTAVTVGFALGYLVSRYQQLILSQSKIDEFINYAQAWIREQGPKITDPIKQ
                     GLESTGSSVEQAIKKVSASRPLESLYLFQRPKHRKFLGIDIF"
BASE COUNT          413 a          489 c          459 g          386 t
ORIGIN      
        1 gtctaccgag cgtccggaga ctcttacatc ctggtggagt acggtccact tgttctggat
       61 ttgaatctcc gtttccgtgt ccatgccctg atgcaattgg tgcagggcca tcgactttcc
      121 gggattgtcg atctgacccc cggtattcga tcgttgcagg tccacttcga ccccaaaaag
      181 gttcccctga gggagttact cgacgtcctg ctgaccgccg aaaaagaact tccgctcgct
      241 gacgagatgg aagtgccgac acgcattgtc catctgccac tgtcctggga agatgaatca
      301 accttgctgg cgattcagaa atatatgcag tccgtgcgcg ccgatgcacc gtggtgtcca
      361 agcaacctgg agttcatccg gcgcgtgaac ggtctaagaa gcatcgcaga ggtaaaggat
      421 atcgtgtttg atgccagcta tctcgtgttg ggtcttggcg atgtatacct gggcgccccc
      481 gtcgccattc cgatcgatcc gcgtcatcga ttggtgacca caaaatacaa tccggcaaga
      541 acttggaccc cggagaatgc tgtcgggata ggcggcgcct acttgtgcat ctacggaatg
      601 gaaggtcccg gcggttacca attcgtcggt cgaacggttc aggtttggaa ccgtttccac
      661 gtcacgaaga atttcgaggc cggtaaacct tggctactgc gatttttcga tcaaattcga
      721 ttctatcctg tttctgcgga gaaattgctt cgtcaccgag aagatttcct gtccggcaaa
      781 ttcaacctga cgattgagga ggaaacattc cggctccgcg attaccacgc cttcctgaaa
      841 cggattgtac ccgaggccgc ggcatttaag gcagaccaac aagcagcgtt cgaagcggag
      901 cggcaacgtt gggcctccac ggaattcgtt ccagcggacg acacgagttc aatggcgctt
      961 caggaagagg tacagttgcc cgaaggcgga tcgttcatcg acgcgcccgt tgccggcaac
     1021 ctgtggaaat tggtgacaaa gccaggagat caggtccagc cggaccagcc ggtggcgatc
     1081 atcgaatcga tgaagatgga aatgcaggtc tgcgcaacaa gcctcggcac cgtccattca
     1141 gtattggtca aagaattttc cccggtctct caaggacagc atctcttcat catcgagagt
     1201 ccgaggcttt gaatcgattc caccaggtcc tttcctcttg cctcaaccgg gggtatactc
     1261 cggaactttg aagagagctt ccaacatcag cctcttaagc caaggagcaa aactatgagc
     1321 gaatttccgg aaatccaacc gttcggacca aggcccgcgc agcacaacga agatccccaa
     1381 aatgcagcac cggcggccca tccctctgag cccccgactc cttctccgag tcaagctccg
     1441 gcaagccgcg tgaatattac ggtggttgtc acagcggtaa cggtcggatt tgctctaggc
     1501 tacctggtct cccgctacca gcaattgatt ctcagccaaa gcaagatcga cgaattcatc
     1561 aactatgctc aagcttggat ccgggaacaa ggcccgaaga ttacagaccc gatcaagcaa
     1621 ggcctggaat ccacggggtc cagtgtggag caggcgatca aaaaagtgag cgccagtcgt
     1681 ccgttggaat cgctctatct gtttcagcgg ccaaaacacc gaaaattcct cggaatcgat
     1741 attttct
//
LOCUS       DATMKH010000709         3919 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2558993, whole genome shotgun sequence.
ACCESSION   DATMKH010000709 DATMKH010000000
VERSION     DATMKH010000709.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 3919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3919
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2558993"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="VNV63_08385"
     CDS             <1..404
                     /locus_tag="VNV63_08385"
                     /inference="COORDINATES: protein motif:HMM:NF012799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HXC62678.1"
                     /translation="YLIRRCIEEGIPTIPVPGPTAATTALVISGLPTDTFLFLGFLPK
                     KRVARLKRLALFKNTLATLILYESPYRVAKTLQDCLDALGNRQAVIARELTKVFEEVM
                     RGSLEELLARLQAKPCRGEITLVIEGQRSEK"
     gene            complement(435..683)
                     /locus_tag="VNV63_08390"
     CDS             complement(435..683)
                     /locus_tag="VNV63_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007223001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HXC62679.1"
                     /translation="MDKNQEQNAAAEIDLRGVICPYNYVKTKLKLEEMSLGQVLAVIL
                     DEGEAMQNVPRSVQDEGHTIIQQERYQSGYRIFIRKTV"
     gene            complement(701..2356)
                     /locus_tag="VNV63_08395"
     CDS             complement(701..2356)
                     /locus_tag="VNV63_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017951749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HXC62680.1"
                     /translation="MSTMTDALTEVFAYHEATKHHLHRYAPGPGGLDWATQPDPFRRY
                     AGASLLKLGHILPGDHPLFEPVFVEGHLPSQPLDRTSISRLFYDSLALSAWKEIGSVR
                     WPLRVNPSSGNLHPTEGYLVCGPIVSLCEQPMVCHYAPKEHALERRAEFSFETWTALT
                     SNLPAGSVLIGLTSIYWREVWKYGERAFRYCQHDAGHAIAAVTLAAAGLGWKATVLDD
                     LSTDMLTNLLGLFDSNGAEQEQPECLLAVYPQGVTSAGLTLPDDVIAAFRTVRWHGTP
                     NQLSPGHVDWPIIDQVTAATRKPLTRQIYGAQTPIGNPLEVGSAPISFSKMVHQRRSC
                     LALDGHTGIPRTAFYQILQKTIPGPGQFPFNALPWPPAIHCGLFIHRVQDVDPGLYFL
                     VRDPSRLETLKNHMKKEFVWEKPAGCPDGLELYRLLTGDARELARQIACHQEIAADGC
                     FSLGMIAEFEEPIRRYGAWFYPRLFWEAGAIGQVLYLEAEAYGIRGTGIGCFFDDPMH
                     MVLGLTDRRFQDLYHFTIGGPVEDKRLTTLPAYPVTNEVEKEL"
     gene            complement(2372..3748)
                     /locus_tag="VNV63_08400"
     CDS             complement(2372..3748)
                     /locus_tag="VNV63_08400"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXC62681.1"
                     /translation="MAPTDQYKKLGLAIVGGGRGGLELLRLFHQEKGIEILGIADKRP
                     EAPAIKFAQSVNIPTTTDFGQLIHRDGVDLIADLTGNPDVRLQIVKDKAPHVELLGGV
                     SAKLMWNFIEMLERKVEERTQELKEVQEELLRNKLAMLQQISSGVSHELQSPLGVIKN
                     SVAYLSDKMTDYPKNIKHLKIIDQEIIIAEKIINDLADFVKPVDWIFASNDLQQQLIQ
                     SEKLAQVGIMAAGIAHEVNNPLSVMLGKAEMILEEEDPARVKKYAQEIIKYAKKASDI
                     VKGITFYSRVTTAPGTERRIYLNAQLNEAIKLSKYSTSFDDIELVTDYQDIPPVRGNA
                     GEIQQVFVNLMKNAVQAMNGKGRLLVSSRYEDGSVVVTIRDTGAGIKKEHLKQIFTPF
                     FTTKDPGRGTGLGLNIVHKIITNHGGSISVESEEGQGAAFIVRLPPTNESPPGEDERW
                     KMGVEKDL"
BASE COUNT          869 a         1101 c         1015 g          934 t
ORIGIN      
        1 tttacctgat ccggcgctgc atcgaagagg gtataccgac gatcccggtc cccggtccaa
       61 ccgcagccac gaccgctctc gtcatctccg gcctgcccac ggacaccttt ctttttctgg
      121 gcttccttcc caaaaagcgc gtggcacgac taaaacgtct tgccttgttc aagaatacac
      181 tggccacttt gattctctac gagtcgcctt atcgtgtggc aaagacgttg caagactgtc
      241 tggatgcctt gggaaaccgg caggcggtta tcgcgagaga gctgaccaaa gtcttcgaag
      301 aagtgatgcg tggaagcctg gaagaattgc tggcccgcct tcaagccaaa ccgtgtcgcg
      361 gagagatcac gctggttatt gagggacaac gttcggagaa atgatgcttc accggccgct
      421 tcctagaaca aaacctatac cgtttttcga ataaatatac gatagccgga ctggtatcgc
      481 tcctgctgga taatggtatg cccttcatcc tgaacgctgc gagggacatt ctgcattgcc
      541 tctccttcgt ccagtatcac tgccaggact tggccgagtg acatctcttc aagcttgagc
      601 ttagtcttga cataattgta agggcatatc accccacgga gatcaatctc ggcggctgca
      661 ttctgttctt ggtttttatc cataaaactt tcctgtccct ttataactct ttttcaactt
      721 cattcgtcac cggataggcc ggcaacgtcg taagccgttt gtcctccacc ggcccgccga
      781 tcgtgaagtg atagagatct tggaagcgcc ggtcggtcag gcccagtacc atgtgcatcg
      841 gatcatcgaa gaagcagccg atgccggtgc cgcgaatccc ataggcttct gcttccagat
      901 agagcacttg gccgatcgcg cccgcctccc aaaagaggcg cggatagaac caagcgccgt
      961 accgccggat cggctcttca aactcggcga tcatccccag gctaaagcat ccatcggccg
     1021 cgatctcctg atggcacgcg atctgccgag ctagctctcg cgcatcaccg gtaagcaggc
     1081 gataaagctc aagcccatcc ggacagcccg ccggtttctc ccagacaaat tcctttttca
     1141 tgtggttctt caacgtctca agacgggaag gatcacggac caggaaataa aggccggggt
     1201 cgacatcctg gacccggtga ataaacaacc cgcagtgaat agccggaggc caaggcagcg
     1261 cattgaaggg aaattggccg gggccgggaa ttgtcttttg caggatttga tagaacgccg
     1321 tccgcgggat gccggtatga ccgtccaggg caagacaact tcgtcgctgg tggaccatct
     1381 tggaaaacga gatcggcgca gagccgacct ccaacggatt tccgatcggc gtctgtgcgc
     1441 catagatctg acgggtcaac ggctttcggg ttgccgccgt cacctgatcg atgatcggcc
     1501 aatcgacatg accgggactg agttggttgg gcgtcccgtg ccagcgaact gttcgaaagg
     1561 ccgcaatgac atcgtctgga agcgtcagcc ccgcagaggt caccccctgc ggataaactg
     1621 ccaagaggca ttcgggctgc tcctgctcgg ccccgtttga atcaaagaga cccagcagat
     1681 tggtcaacat gtcggtcgaa agatcatcca acacggtcgc tttccatccc aagccggctg
     1741 cggcaagcgt gaccgctgcg attgcatggc cagcatcgtg ctgacaatag cgaaatgccc
     1801 gctcgccata cttccacacc tcccgccagt agatcgaggt caatcctatg agaaccgagc
     1861 ccgccggcag attcgacgtc aaggcggtcc acgtctcaaa tgaaaactca gcgcgccgtt
     1921 ccagcgcatg ttccttcggg gcgtaatgac agaccatcgg ctgctcacag agactgacga
     1981 tcgggccgca gaccagataa ccttccgtcg gatggagatt gccgctggaa ggattcacac
     2041 gcagcggcca gcgcacgctg ccaatctcct tccaggccga gagggccagg ctgtcataga
     2101 agagccggga gatgcttgtt cgatcaagcg gctgggacgg cagatggcct tcaacaaaga
     2161 ccggctcaaa caacggatga tcacccggca gaatatgacc gagcttcagc aatgacgcgc
     2221 cggcgtagcg ccggaatgga tcgggctgcg tcgcccagtc aagcccgccg ggtcccgggg
     2281 cataccgatg cagatggtgt ttggtcgctt catggtaggc aaagacttcg gtcagcgcat
     2341 cagtcatggt ggacatggaa gatctttttc tctagaggtc cttttcgacc cccattttcc
     2401 atcgctcatc ctcccccggc ggggattcgt ttgtgggtgg gagcctcacg ataaaagcgg
     2461 ctccttggcc ctcttcactt tccacgctga tgctcccgcc gtggttcgtg atgattttgt
     2521 gaacaatatt taaaccgagg ccggttcccc ttccaggatc cttggtcgta aaaaaaggcg
     2581 tgaaaatttg cttgaggtgt tcctttttga tcccggctcc tgtgtctcgg atggtgacca
     2641 ccacagagcc atcctcgtat cgtgacgaca caaggagacg gccttttcca ttcatggctt
     2701 gaaccgcatt cttcatcaag ttaacaaaga cctgctgaat ctccccggca ttgccgcgga
     2761 ccggaggaat gtcttggtag tccgtaacca gctcaatatc atcaaaggag gtcgagtatt
     2821 tagaaagctt tatcgcctcg ttgagttgag cgtttagata tatccggcgc tcggttccgg
     2881 gggcggtggt aacccgcgag tagaaggtaa tacccttgac aatatcggag gcctttttgg
     2941 catatttgat aatttcttgg gcatattttt taacgcgagc cgggtcctct tcttcgagga
     3001 tcatctcggc tttgccaagc atgacggaaa gcgggttgtt cacttcatgg gcgataccgg
     3061 ccgccatgat accaacctgg gctaatttct cggattggat gagttgctgc tggagatcgt
     3121 ttgaagcaaa gatccagtcc acgggcttga caaagtcggc caggtcgttg atgatcttct
     3181 ctgcaatgat gatctcttga tcgatgatct ttaggtgttt tatgtttttg ggataatcgg
     3241 tcatcttatc ggagagataa gcgaccgaat tcttaatgac ccccagcgga ctttgaagtt
     3301 cgtgcgagac acccgatgag atctgttgca gcatggctaa cttatttctc agcaactctt
     3361 cttgaacttc tttgagttcc tgcgtccgct cctccacttt gcgttccaac atctcaataa
     3421 aattccacat gagtttcgca ctgacccccc ccaagagttc cacatgagga gctttatcct
     3481 ttacaatctg taaccggaca tcggggttgc ccgtcagatc cgcaatgagg tcaaccccat
     3541 cccggtggat cagttgtcca aaatcggttg tcgtgggaat gttgacggat tgggcaaact
     3601 ttatggcagg ggcttccggg cgtttgtctg cgatgccgag gatctcgatg cctttttctt
     3661 gatggaagag ccgaagcagc tccagaccgc cccgccctcc tccaacaatg gccagtccta
     3721 atttcttgta ttgatcggtc ggcgccacga tttcatcaag ccagctcagc catctccaag
     3781 attacaatct gcgtttctac agtctttgtt tctgccaatg tcttcgcgta aattccccaa
     3841 gttacattat gcccataaga caaattacgg tcaaggtcat aattgctcag cccttcccca
     3901 tcttagacat ctcatgctt
//
LOCUS       DATMKH010000710         1109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_1923922, whole genome shotgun sequence.
ACCESSION   DATMKH010000710 DATMKH010000000
VERSION     DATMKH010000710.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1923922"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(512..712)
                     /locus_tag="VNV63_08405"
     CDS             complement(512..712)
                     /locus_tag="VNV63_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62682.1"
                     /translation="MLDHLSEFRSEELSNDGVLARVLAGQKVASALIDRAMKTLAPLE
                     IKREELSQFIMEQIKKKSQRDR"
BASE COUNT          267 a          297 c          287 g          258 t
ORIGIN      
        1 ttgtgggttc gatccccacc gccccctacc aaccatcgtc ctgattggtg ggaacttacc
       61 aagaatgcga gggggcaaaa gggggcaaat aatctcgatg atccggttct tgcgtgaatt
      121 ttcggcggcg cgagtgacaa cctgattcct ggggaaagga tccctaagaa agcacgggtt
      181 gcctcgaact ccgatccaat ggtagcttcg ctcagtcgtg gaggatccga aagcaagtcc
      241 gcaacagcac aagtatcgtt aacacgccca tacaaaacac ggcagttgca gacgcgattt
      301 cgaggtttgg ctagggagat agcaggactg gtcagagtgc gaacaaacag ccgcggattg
      361 aggtaaacca tgaatgcgaa aacacaatca gatagggctc caaaattgga gtagccaagt
      421 cgttcccacc ggtaggccgg ctacctcaaa cgcaggagtt agatgccggc gcacgtcgat
      481 tgaaactccg aatttggaga acgctctgtg cctatctgtc ccgttgcgat ttcttcttga
      541 tctgctccat gatgaattgg ctcaattctt cgcgcttgat ctccaatggc gccagcgttt
      601 tcatagctcg gtcaatcagc gcggaggcta ctttctgtcc agcaaggacc cgcgccaaaa
      661 ccccatcgtt gctgagttcc tcagaccgga actctgagag gtggtccagc acatccgcta
      721 aggaaaaatc gatggagagg tcagcgatcc gggccccttc cccatgaatc cctcacgatt
      781 catagggttc acgttagtct gaactgcgac atcgaagacg gtcttgtaga cttcctcgtg
      841 gattgtcttg agtttccaca aagtctggta tctctgcccg cacctgccga tggcctactg
      901 gaaggtcgct ctcctgggtg ggcaaccaga caattccaag catatattct gtttcgactg
      961 tatcgaggat caagatctcc gcacatctct ttcgacgtca atcctggatg tcagagcctg
     1021 tctacctgct tcgatgaagg acatccccta gatcttcacc tccgaagcgg ttttcttcaa
     1081 ggtcagatag aactttggcg aggagtagg
//
LOCUS       DATMKH010000711         1129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4798680, whole genome shotgun sequence.
ACCESSION   DATMKH010000711 DATMKH010000000
VERSION     DATMKH010000711.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1129
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4798680"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(489..770)
                     /locus_tag="VNV63_08410"
     CDS             complement(489..770)
                     /locus_tag="VNV63_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62683.1"
                     /translation="MLRITVVESSKTAVTLRVEGRITGLWVEELRTACNLHTFSDEVR
                     LSLELADISFADTAGIALLRELRNRGVGLIRTAPFLAELLKDGTSYLKF"
BASE COUNT          257 a          309 c          300 g          263 t
ORIGIN      
        1 caaaccttta tgatggtgct tgatcatgat gtggcggaac tcctttctca cacttataat
       61 ccggatcgca cgctcgactg tttcaacgat gcttcggacg gcgggccgaa gagtgtcgca
      121 acccatggag cactccaaca atgaagtgac cagttcattc atccgctcga tggaccaacg
      181 gatttcctgg taaagttcgt tcctttctgt ttcgctgata tctgactggg tgagaaattc
      241 tgcattcgcc aggatggcgg tcaaaggaag gcgaagatcg tggcaaacga gggccaccat
      301 cttgcttgcg tcggattgcg gctccaaaat gagaatattt ctttggacgt accgcaaaga
      361 ctcggccggc aataaccgct ccctacacac agtttgttcc agcattgaaa accccctacc
      421 gtctgtcgat gggccgaagt tcagagatgg aggatccctt ccggcttcgg tatgcgctcc
      481 tttgtgccct agaatttgag atatgaagtc ccatccttga gcagttcggc caaaaacggc
      541 gccgtgcgga tcagaccgac gccgcggttc cgcagttctc tgaggagagc gatccccgct
      601 gtgtcggcga atgagatgtc cgctagttcg agggacaacc gaacctcatc ggaaaaagta
      661 tgtagattgc atgctgtgcg tagctcctcg acccagagac ccgttatccg gccttctacc
      721 cgaagagtta ccgcagtctt tgatgattcg acaaccgtga tcctcagcat tgcggtttac
      781 ctgattgcct ctgctgctca ataaagcaat cgacatgcca tccagaagat cagaccaggc
      841 ctgaagcgat ctcccgagat ccactagacc gtccgagcca acatctcaat aaaatcaata
      901 ggttgggaag gtttgcagtc atctgtcctc gctccggctg ccaggatgcg cagacggctg
      961 tttcgtccgg ggccctggac tgcgagacgg attcaccaca ttcggtaaac tcgggccagc
     1021 tggtgtagga ggaactacga caggcggtgg cgagatccct tgatgccgag cttatccatc
     1081 cggtggcgaa gcgtgtttgg atgaagattc aggatcctcg ccgcgcctt
//
LOCUS       DATMKH010000712         5069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3844780, whole genome shotgun sequence.
ACCESSION   DATMKH010000712 DATMKH010000000
VERSION     DATMKH010000712.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 5069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5069
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3844780"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="VNV63_08415"
     CDS             <1..332
                     /locus_tag="VNV63_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXC62684.1"
                     /translation="SGQVTRLCLAKALLNDPKILFLDEPTVSLDPDMADKTRSLLKGL
                     RSRTGLTILYTSHNMREMEEMCDRILFLNKGHLIAAGTPQELLKQFGKENLEEVFLKI
                     AREKEAS"
     gene            329..1120
                     /locus_tag="VNV63_08420"
     CDS             329..1120
                     /locus_tag="VNV63_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009382771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXC62685.1"
                     /translation="MKLHRILAIALRHLYLYKRSLSRLMEIFYWPLLDLLVWGFITIY
                     LEQFRGTLPNFVAFFLGALILWDILYRSQQGISVSFLEEVWSRNLLNLFVSPLRPGEF
                     LIATMLISVFKLLSAALVTVVLAWLMYSFNIFLIGVALIPFVLNLILMGWAIGIVTTT
                     LILRYGQKAEILAWGLAFLFQPVSAVFYPVSVLPIFLQKIAWFIPATHVFEGMRSVIR
                     KQGLPIEHLVWAFGLNTVYLLAAVLLFYHIFTIVRQKGLLLRVGE"
     gene            1237..2088
                     /locus_tag="VNV63_08425"
     CDS             1237..2088
                     /locus_tag="VNV63_08425"
                     /inference="COORDINATES: protein motif:HMM:NF024762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HXC62686.1"
                     /translation="MKQITDNQLRALFKLLSDDDEKIAKLVSQQILQVGEPAIPLLEQ
                     TAAEDSLIGDRARAVLAVLQFKELEEAFLDFSAKDDAGLDLEEGAFLIARFGYPQMKP
                     TNYQALLDDMATEIAPNIRRKRLPEEILRTINNYLFLEQGFVGNTDHYYDPDNSYINR
                     VLDRRTGIPISLSIVYLLIAKRLELPVFGIGMPGHFLVKYSDKKNEILADPFNKGRLL
                     TKTDCANFLINSGYDFEESYLAATPNRTILSRMIRNLISVYTKQKEIRLAEHLGKLLA
                     ILEKPQG"
     gene            complement(2113..2667)
                     /locus_tag="VNV63_08430"
     CDS             complement(2113..2667)
                     /locus_tag="VNV63_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HXC62687.1"
                     /translation="MEMTLLLNATYEPLRVVHWQKAITLLWQGKVEVLEVYDKEIRGI
                     SITIKLPAVMRLLKLVKVRNGHGMVKFSRSNIFARDKYSCQYCGQHYRTEDLTFDHVI
                     PIAKGGRKTWENIVTACWRCNNRKSGRTPEEAGMRLIKKPIKPKWTPTITITIGIKNA
                     PESWRDYLYWNVELDKDNGEEFLT"
     gene            complement(2759..3850)
                     /locus_tag="VNV63_08435"
     CDS             complement(2759..3850)
                     /locus_tag="VNV63_08435"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HXC62688.1"
                     /translation="MKRLALHDKHESLGAVFGERDGWQVPLHYGSAVVEHRAVRRTGG
                     LADRSYRGKLRFAGKDRITFLQGQITNDVKQLTEGQGLYAALLTIKGKMVADLALFQE
                     GDAILMDLEPALTEKIRVQLSRYILAADVKIEDVSEKTAHLTLQGPASADLLVKLSGE
                     SLPPLSEHSVVRRSLKGHPVMIIKTAYTGEEGYDLLMPVADASNVWNLLMEAGHGIGI
                     RPIGFEALDTLRIEAGIPRYGVDMDENAFPVEVGLEEKAISYTKGCYTGQEPISRIKH
                     RGQVNRHLIGFILEGSTPPQKGDIVMKDGQEVGNITSGIVSPTLEKTIALGYLRRELA
                     EAGHAVTILHVEKPIPASVATLPFYQRKK"
     gene            complement(4107..4475)
                     /locus_tag="VNV63_08440"
     CDS             complement(4107..4475)
                     /locus_tag="VNV63_08440"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXC62689.1"
                     /translation="MILVVNDDLIWGTKIAEAVLQAGLQYLLAKTEQAAMDQARLLRA
                     SLIVIDLGVKSLDPMALIKRLKQDAELKEIPVLGYTFHTDQVTWQKGVAAGCDQVVAR
                     SALQQALGKAIAKIKNAGAI"
     gene            complement(4472..4780)
                     /locus_tag="VNV63_08445"
     CDS             complement(4472..4780)
                     /locus_tag="VNV63_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009382038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HXC62690.1"
                     /translation="MVLLEFSMSPMDKGESVGKYVARSLEIIDKSGVDYRLNPMGTVL
                     EGEWDQVLGVVRSCFERMSQDCNRVTATIKVDYRKGQTGRLTGKVASVEKQLGRKLKT
                     "
BASE COUNT         1151 a         1437 c         1226 g         1255 t
ORIGIN      
        1 cgtccggcca ggtcacgcgg ctctgcctcg ccaaggcgct tctcaatgac ccgaaaattc
       61 tgttcctgga cgagcctacc gtgagtctcg atccggacat ggcggataag acccgaagtt
      121 tactcaaagg gctccgaagc cgcaccggcc tcacgatcct ttacacctcg cacaacatgc
      181 gagaaatgga ggaaatgtgc gaccgcatac ttttcttaaa caagggtcat ctcattgcgg
      241 ccggaacccc gcaagaactg ctgaaacaat ttggaaaaga aaatcttgaa gaagtattcc
      301 tgaaaatcgc cagagagaag gaggcatctt gaaactccac cggattcttg cgatcgcgct
      361 acgccatctt tatctttaca aacgcagcct ctcgcggctc atggagatct tttattggcc
      421 gcttttggat ctcctcgtct ggggctttat cacaatctac cttgagcagt tccgcggtac
      481 gctccctaat tttgtggctt tctttctggg tgcgctgatt ctctgggata tcttgtatcg
      541 ttcacagcag gggatctccg tctcgttttt agaagaggtc tggtcgagaa acctcttaaa
      601 cctattcgta tccccgcttc ggcccgggga atttctaatt gccacgatgt tgatcagcgt
      661 ctttaaactc ctctcggccg cgctcgtgac ggtagtactg gcctggctga tgtattcgtt
      721 taatatcttc ttaatcggcg tggccctgat cccgttcgtc ttgaacctga tcctcatggg
      781 ctgggcgatc ggaatcgtga ccacaacgct catactccgg tacggccaga aggcagaaat
      841 tctggcctgg gggctggcct ttctatttca accggtctca gccgtgttct acccggtctc
      901 tgtcctgccg atattcctcc aaaaaatcgc ctggtttatc ccggcaacgc acgtctttga
      961 aggaatgcgt tccgtgattc gcaagcaggg cctaccgatt gaacatcttg tctgggcctt
     1021 tgggctaaat acggtctatc ttcttgccgc tgtcctgctc ttctaccata ttttcacgat
     1081 cgtgaggcag aaaggtttac tgctccgagt cggtgaatga gcacctgcag gcaccgtgac
     1141 aattcaattt ctgctccggc tgtcgtacat tatattcatc agaatattca tcagaaaaag
     1201 gttgcttgat gaatgttctc ttgttagaat aatcacatga agcagatcac agacaatcag
     1261 ctccgggcgc tcttcaaact actctcagat gatgatgaga agatcgccaa actcgtctca
     1321 cagcagatcc ttcaagtagg tgagcctgcg atcccgcttc tcgagcagac cgcagcggaa
     1381 gattccctta tcggtgaccg ggcacgggcc gtgttggcag ttttacagtt taaggagctc
     1441 gaagaggctt tcttagactt ttcggcaaaa gacgacgctg ggctggatct cgaagaaggc
     1501 gcttttctca tcgcccgctt cggttatccc cagatgaagc cgacaaacta tcaggcgctg
     1561 ctggatgaca tggccacgga gatcgcacca aacatccggc gaaagcgtct tcctgaagaa
     1621 attcttcgga cgatcaacaa ctatctgttt ctggagcagg gctttgtggg caatacagac
     1681 cactactatg atccggacaa cagctacatc aaccgggtgc tcgaccgccg gaccggaatt
     1741 ccgatttccc tctccatcgt ctatctcttg attgcgaaga ggctggaatt accggtcttt
     1801 ggcatcggga tgccgggcca tttcttggtg aaatattccg acaagaaaaa tgagatcctg
     1861 gcggatccct tcaataaagg gcgcctcctg acgaagacag actgcgccaa ctttctgatt
     1921 aattcaggct acgattttga agagagctat cttgccgcca cacccaacag gacgatactc
     1981 agccggatga tccggaacct gatctcggtc tatacgaaac agaaagaaat ccgtctcgca
     2041 gaacatctcg gaaagcttct tgccattctg gaaaaaccgc aaggttagaa aacctgctat
     2101 agggaaggat ttttaggtaa gaaactcctc gccgttgtct ttgtccagct cgacattcca
     2161 gtagaggtag tctcgccagc tctcgggcgc gttcttaatc ccgatcgtga tcgtgatggt
     2221 tggcgtccat ttgggcttga tgggtttctt gatcaaccgc atgccggcct cctccggggt
     2281 gcgcccgctc ttgcggttgt tacacctcca acaggcggtc acaatatttt cccaagtctt
     2341 gcgcccaccc ttggcaatcg ggatgacatg atcaaaggta agatcttcgg tgcgataatg
     2401 ctgaccgcag tactgacagg agtatttgtc tcgtgcgaag atgttgctgc gtgaaaactt
     2461 gaccatgcca tgaccgttcc gaaccttgac gagtttcaga aggcgcatca cagcgggcag
     2521 cttgatcgtg atcgagatgc cgcggatttc cttgtcgtag acctccagca cctccacctt
     2581 gccctgccag agcagggtga tcgccttctg ccaatgcacc accctcaagg gttcgtaagt
     2641 agcgtttaaa agcagtgtca tttccatgac aaggtctcga tcttcattat aattataacg
     2701 tatttttaaa aaaaatccag ctctgccttc ccctctcgct cgccgtgctc gctggcagct
     2761 atttcttccg ttggtaaaat ggcaaagtcg cgacggaggc cgggatcggc ttctccacgt
     2821 gaaggattgt gacggcgtgg cccgcctctg ccaattcccg acgcagatag cccaaggcaa
     2881 tcgtcttctc cagggttggg gagacaatcc cgctcgtgat attacctact tcctgcccgt
     2941 ccttcatcac aatgtctccc ttttgcggag gtgtcgagcc ctcgagtata aaaccgatta
     3001 gatgccgatt cacctggccg cgatgcttaa ttcgggagat cggctcctgt ccggtgtagc
     3061 agcccttggt ataactgatc gccttctctt caaggcccac ctcaaccggg aatgcattct
     3121 catccatgtc cacaccgtat cgaggaatcc cggcctcgat acggagcgta tcgagcgcct
     3181 caaaaccgat tggacgaata ccgattccat gcccggcctc catcaaaagg ttccagacgt
     3241 tggatgcatc cgcaaccggc attaaaaggt catacccttc ttcgcccgtg taagcggtct
     3301 taatgatcat cacgggatgg ccctttaagc ttcttcgaac cacgctatgc tctgaaagcg
     3361 gcggcaggct ctcaccagac aacttgacta aaagatccgc ggaagcaggc ccctggagtg
     3421 taagatgcgc tgtcttctcg cttacatctt cgatcttgac gtccgccgca agaatatatc
     3481 gggaaagctg aactcggatc ttctcagtaa gtgcaggttc cagatccatc aggatcgcgt
     3541 cgccctcctg gaaaagcgct agatccgcca ccattttccc cttgatcgtg aggagcgccg
     3601 catacaggcc ttggccttcg gtgagctgtt taacatcgtt tgtgatctgt ccctgaagaa
     3661 aggtgatccg atcctttccg gcaaatctga gcttgcctcg ataagatcga tctgccaggc
     3721 cgcccgtacg acggaccgcc cgatgctcca caacagcgct gccgtaatgg agcgggacct
     3781 gccagccgtc ccgttccccg aagaccgcgc ccaaggattc gtgcttgtca tgtagtgcca
     3841 atcgcttcat ttaatcgtcc aacaatcggg aatgattata caggattcgc aaagtcacgt
     3901 tcaagggagg gccggcttac ggttgacaat gaccttcagc cctgctatct tttttctatg
     3961 gatcatatca aaacaaatct cgtgtcgtgg gccgatcgcg aaggacctct cccggagcgg
     4021 tgcgatctct gcgacaccgt catgatcagc atccattgca agatcacctg cctgcgctgt
     4081 ggctataaac gtgactgctc ggatccctaa attgctcctg cgttcttgat cttggcgatg
     4141 gccttcccga gcgcctgttg aagggcggat cttgccacca cctgatcaca gccggcggca
     4201 acaccctttt gccaagtcac ctgatcggtg tggaaggtgt agccgaggac cgggatctct
     4261 ttaagttcag catcctgctt cagacgcttg atcaaagcca tgggatccag actcttgacg
     4321 ccgaggtcaa tcacgattag cgacgcccgg aggagccggg cttgatccat cgccgcctgc
     4381 tcggttttcg ccagaagata ctgaagtccg gcctgcagga cggcttcggc aattttggtc
     4441 ccccaaatca aatcgtcatt gacgactaaa atcatgtctt gagtttcctg cccagttgct
     4501 tctcaacgct ggctaccttg ccggtcagcc ggccggtctg cccctttcgg taatccacct
     4561 tgatcgtagc cgtaacccga ttgcagtcct ggctcattcg ctcaaagcaa gatcgaacga
     4621 cacctaagac ctgatcccat tctccttcca gaaccgtacc catcgggttc aaacggtaat
     4681 ccaccccgct tttgtcgatg atctcaagag atcgcgccac atactttccg acgctctccc
     4741 ccttgtccat cggtgacatg ctgaattcta agaggaccat ggcagctcct ttcttaaaga
     4801 aagtgctatt atatcacaaa gttgcttgta aactccgagg agtggaaagt tgcttgtaaa
     4861 ctccgaggag tggtatgatc aacaaacaat cacagaagag aactccatgg ccaagaaaga
     4921 gatgatccac accgtcgtgg aggcgattac cgctctcaac cccacgacca aaatgtttcg
     4981 ccttcggctc ccgccggata agaccctgcc gttcaagccg ggacagtttg taatggtcca
     5041 tgcccccaaa gacgggaaac tttcctcca
//
LOCUS       DATMKH010000713         1406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2242712, whole genome shotgun sequence.
ACCESSION   DATMKH010000713 DATMKH010000000
VERSION     DATMKH010000713.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1406
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2242712"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="VNV63_08450"
     CDS             <1..726
                     /locus_tag="VNV63_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010866869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HXC62691.1"
                     /translation="TPVAGASLAVSSRKILEKAKKIAAHLIEARPEDVVMENGRFHVR
                     GSKEPSKGWGDVAYAAYGAGSNEIPKGMEPGLEDITFYDPVNFVFPFGTHICIVEVSR
                     QTGEVKIKRYVAVDDCGRQINPMLVEGQIHGGITQGISQALWEECVYDQDGNLITGSL
                     VDYAVPTAVEVPHYELSHTVTPSPHNPIGVKGIGEAGTIAATPAVVNAVVDALSHLGV
                     THIDMPLRSDKIWEILEEKGAAQ"
     gene            731..1129
                     /locus_tag="VNV63_08455"
     CDS             731..1129
                     /locus_tag="VNV63_08455"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HXC62692.1"
                     /translation="MKRILFSSELPASNFYSQGIKVGNTIYLSGIVAFDPQTKKVETK
                     SIEEQTERAIRNCELILREGGGNLGDVVQVIVLLKNPEDFDGMNRAYLKIFPNDSLPT
                     RAVTKLGVDLPNILVSVMMTAVLQEGPTQR"
     gene            complement(1133..>1406)
                     /locus_tag="VNV63_08460"
     CDS             complement(1133..>1406)
                     /locus_tag="VNV63_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62693.1"
                     /translation="VPIEIYFTASSQKALKPLISNISEKFDDSLLLIETYVGPSVNSL
                     FICKNVAAAEDIFQYVRSQDDITIADFHLIRGAVRVRDKLRLNLKI"
BASE COUNT          356 a          318 c          419 g          313 t
ORIGIN      
        1 acgccggttg cgggagcgtc gctcgctgtt tcgtcaagga agatactcga gaaagctaag
       61 aagatagcgg cccatctcat cgaggcgaga ccggaggacg tggttatgga gaacgggcga
      121 tttcacgtca ggggatcgaa ggaaccgtcc aagggctggg gagacgttgc ttacgctgcc
      181 tacggtgcgg ggtcaaacga gattccaaag ggaatggaac cgggcctcga ggatatcacg
      241 ttttacgatc cggtaaattt cgtgttccca ttcgggacgc acatctgcat cgtcgaggtc
      301 tcgcggcaga ccggcgaggt gaagatcaaa aggtacgtgg cggtcgacga ttgcggacgg
      361 cagatcaacc ccatgcttgt agaggggcag atccacggcg ggatcacgca agggatctcg
      421 caggcgctct gggaggagtg cgtttacgac caggacggaa atctcattac cggctcgctg
      481 gtcgactatg cggttccgac agcagtggag gtgcctcact atgagctgtc ccacacggtg
      541 acgccgtcgc cgcacaaccc gattggagta aaggggatcg gggaagccgg aacgattgca
      601 gcgactcctg cggttgtgaa tgcagtggtt gatgccctaa gccatctagg agtgacgcac
      661 atcgacatgc ctttgcggtc ggacaagatc tgggagatac tggaagaaaa aggggctgcc
      721 caatgaattc gtgaagcgaa ttcttttttc ttctgaactc ccagcctcca atttttacag
      781 ccaggggatt aaggttggga atacaatcta tctttccgga atagttgcgt tcgatccgca
      841 gacaaagaag gtggaaacga aatcgataga ggaacagacc gaacgtgcga tccgcaattg
      901 cgagctcatc ctgcgggaag gcggggggaa tctgggagac gtcgtgcagg tgatcgtgtt
      961 gctaaagaat ccagaggatt tcgacgggat gaatcgtgct tacctgaaga tctttccaaa
     1021 tgattccctt ccaactagag ccgttacaaa gcttggagtg gaccttccaa atatcttggt
     1081 ttctgtgatg atgacggctg ttttgcaaga aggaccgact caaagataag aattagattt
     1141 tgaggttcag cctcaacttg tctctgacgc ggaccgcgcc ccggatgaga tgaaaatctg
     1201 cgatggtaat gtcatcttgg gatctaacat attgaaaaat gtcctctgcc gcggcgacgt
     1261 ttttgcagat gaacaaactg ttcacgcttg gtccgacgta agtttcaatc aacagaagag
     1321 agtcgtcgaa cttttcagag atattcgaaa tgagcggttt cagggctttt tgagacgaag
     1381 cagtgaagta aatctcaatc ggtacg
//
LOCUS       DATMKH010000714         1584 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4802274, whole genome shotgun sequence.
ACCESSION   DATMKH010000714 DATMKH010000000
VERSION     DATMKH010000714.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4802274"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..>1584
                     /locus_tag="VNV63_08465"
     CDS             71..>1584
                     /locus_tag="VNV63_08465"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009223459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HXC62694.1"
                     /translation="MSLLPNHSLLQPVVRLAGISKSFTGTQAVRNIDLEILPGEVHGL
                     VGENGAGKSTLMRILAGLFPDYEGEIYFKGRPVKIHTPRLARELGIAMVHQELSLVPE
                     LSVAENMFLGREACSRIFGLIDRRRRQKEAGMILSEMQAAISPALKVNQLSIAKQQLV
                     EIAKGISMHSGVLILDEPTSSLTEPEIRDLFRVIRALKEKDVAIIYISHKLAEIFAIS
                     DRITVLRDGARLSSRPVSQWKEADLVKEMVGRALSDFFPRSHQHLHDRAVLEVSHLTQ
                     LPHFIDISFTIHRGEVVGIYGLVGSGRTRLAKAIFGLSRADHGEIRIQGRLVEIRSPS
                     DAIANGVALVPEDRRVLGLIQMLDVRKNLTIPHLKALSNFLLIDDREERELVRENIAD
                     LKIRTSSTKLAVSALSGGNQQKVVLGKWLNTKPAFLILDEPTRGIDVGAKAEIRNRID
                     ALASEGMGILLISSELPEIMGMSDRILVMREKRIAGEFNRHEFNEEVIGSCAIA"
BASE COUNT          381 a          417 c          435 g          351 t
ORIGIN      
        1 ggccgagcct tgacgtgaga aaggtcaaca acgtattcta agcctatagg ctataggaag
       61 tccaaaatcg atgtcgctct tgccaaatca ttctctgctt caacctgtcg tccgcttggc
      121 aggcatcagc aagagtttca caggcactca ggcggtgcga aacatcgacc tcgagatcct
      181 gcccggtgaa gtgcacgggc tggtgggcga gaacggcgca ggcaagtcaa ctctgatgag
      241 aattctggcg ggactgtttc ccgactacga aggcgagata tacttcaaag ggcggccggt
      301 aaaaatccat accccgcgct tggcgcgcga actgggaatt gccatggtgc accaggaact
      361 gagcctggtg ccggagctct cggtggccga aaacatgttc ctgggcagag aagcttgttc
      421 aaggattttt ggactcatcg atcgccggcg aaggcaaaaa gaggccggca tgattctctc
      481 cgaaatgcag gcggccattt ctcctgccct gaaggtcaac cagctctcaa ttgccaaaca
      541 acagctggtc gagattgcga aggggatttc catgcattct ggggttttga tccttgatga
      601 gccaacctcc tcgctcaccg agccggaaat cagggatctt ttcagagtca ttcgcgcgct
      661 gaaagagaag gacgtcgcca tcatctacat ctcccacaaa ctggcggaga tttttgcgat
      721 ttcagatcgc attaccgtcc tccgagacgg tgcgaggctc agttctcgtc ccgttagcca
      781 gtggaaggag gcagacctcg tgaaagagat ggttggaaga gcactgtccg atttttttcc
      841 gcgcagtcac caacatcttc atgaccgtgc cgtcttggag gtttctcacc tcacacaatt
      901 gccccacttc attgacatca gctttacgat ccatcgtggg gaagtggtcg gaatatatgg
      961 gttggtcgga tccggacgaa cccggctcgc aaaggccatc ttcgggctct ctcgagcgga
     1021 ccatggcgaa atcaggatac agggacgttt agtcgagatc cggtcgccct ctgacgccat
     1081 tgccaacgga gtggcgcttg ttcctgaaga ccgacgtgtt ctcggcctga ttcagatgct
     1141 cgatgtcagg aaaaacctga ccatcccgca tttgaaagct ctttccaact tcctcctcat
     1201 tgatgatcgg gaggagcggg aactggtgcg cgaaaatatc gcagatctca agatcaggac
     1261 ttcttcgacc aagttggctg tttcggctct cagcggcggc aatcaacaaa aagtagttct
     1321 gggaaaatgg ctgaacacca aacccgcatt cctgattctc gacgagccga cacgggggat
     1381 cgatgtgggc gccaaagcgg aaattagaaa caggatagac gccctcgctt ccgagggaat
     1441 gggcatcctc ctcatttcct ccgaattgcc cgagattatg ggtatgagcg atcgaattct
     1501 agtcatgagg gaaaagcgga tcgccggcga atttaaccgt cacgaattca acgaggaagt
     1561 gattggatcc tgcgctatag ccag
//
LOCUS       DATMKH010000715         1831 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_3847623, whole genome shotgun sequence.
ACCESSION   DATMKH010000715 DATMKH010000000
VERSION     DATMKH010000715.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3847623"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="VNV63_08470"
     CDS             <1..363
                     /locus_tag="VNV63_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXC62695.1"
                     /translation="GKVVAVDTQERLSAQLRRSDKIGLRVRRAAPDLLSKLQAIPGVL
                     HVLQEPVSAAPAGAPDGQYYLVESQLDHDVREELARCIVSQGCGLLEMKNLTMTLEEV
                     FLRLTTEERSSQKTEAQA"
     gene            360..1130
                     /locus_tag="VNV63_08475"
     CDS             360..1130
                     /locus_tag="VNV63_08475"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HXC62696.1"
                     /translation="MKNLITIMNKELKLYFISPVAYVVGTVFLIIIGFLFYSIVALFS
                     RQSLEFMRLQGTTPQLDIHEAVFRPTFLNMGVILLLVAPILTMRLFSEEKKLKTIELL
                     MTSPVTITEMVLGKYLAAFLVYAGLLALTFCMPILIGVYSVIDWGPLGSSYLGLLLLG
                     GAFLVVGLFTSSLTENQIIAAVISFGVLICLWMLSIAARTVSSSTLGGLLNYLSLYEH
                     LNHFIRGLLDTSDVIFYLSFIAFGLFLTHRVVESQRWR"
     gene            1142..>1831
                     /locus_tag="VNV63_08480"
     CDS             1142..>1831
                     /locus_tag="VNV63_08480"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HXC62697.1"
                     /translation="MQFGSNTVLMVLLFLGILGIINFLGFRHRLRADLSSSHNFSLAP
                     QSVNVAKGLKQEINATGFFQEKSPARTEFKDLMESYRYYNPKIRYELVDPDKDPGTAR
                     RYGITQPDTVVLESGAQDIRVRNISEQELTSAIIRVSREGKRVIYFLEGHGEHGLDDG
                     DRAGYSSAKEGLERQGFEVKSLLLLEQPKVPSDATILVLAGSQRPILKEEQGVLSAYL
                     KAGGQLLVMLDP"
BASE COUNT          397 a          476 c          516 g          442 t
ORIGIN      
        1 ggaaaagtag tagcggtgga tacgcaggaa cgtctctcgg cccaactacg gaggtccgat
       61 aaaatcgggc tcagggtccg ccgggccgca ccggatctgc tgagcaagct gcaggcgatc
      121 cccggcgtac ttcatgtctt acaagaaccg gtgtcggccg ctcctgccgg ggcaccggac
      181 ggacaatact acctggtaga gtcgcaattg gatcatgatg ttcgagaaga gctggctcgc
      241 tgcatagtga gccagggatg cggtctcctg gaaatgaaga acctcacgat gacactggag
      301 gaagtcttcc tccgattgac aaccgaagag cggtcctccc aaaagacaga agcccaggca
      361 tgaaaaacct aatcaccatt atgaataaag aactcaagct ttatttcatc tcgcctgtgg
      421 cctatgtggt gggaacggtc ttcttgatca ttattggatt cctgttctac agcattgttg
      481 cactcttttc ccgacagagt ttggagttca tgcggttgca ggggaccacg ccgcagctgg
      541 acatacatga ggcggtcttc cgtcccacct tcctcaatat gggtgtgatc ctgctgctgg
      601 tcgccccgat tttgacgatg cggctgtttt cagaggagaa aaagctcaag acgattgagc
      661 tcttgatgac ctctccggtg accatcaccg agatggtgtt aggcaaatac cttgccgctt
      721 ttctggtcta tgccggtctt ctggccctga ccttctgcat gccgatcctg atcggggtct
      781 acagcgtcat tgactggggc ccgctcgggt ccagttattt ggggctgctc cttctgggcg
      841 gtgcatttct ggtggtgggg ctgttcactt cctcgcttac cgagaatcag atcatcgcgg
      901 ccgtgatcag ttttggggtt ctgatttgtc tctggatgct cagcatagcg gctcgaaccg
      961 tttcgtcatc cacactggga gggcttctca actatttgtc cttgtatgag cacctgaacc
     1021 atttcatccg aggcctgctg gatacatcgg atgtgatctt ctatctcagc ttcattgcgt
     1081 tcggactatt tctcacacat cgcgtcgtgg agtctcaacg atggagataa ggcggcgctc
     1141 attgcaattc ggatcaaata cggttctcat ggtcctgctc ttcttgggca ttctggggat
     1201 cattaacttt ctaggttttc ggcatcgcct ccgcgccgat ctttcaagct cccataactt
     1261 cagcttggcc ccgcagtcgg taaacgtggc caagggactg aagcaagaaa tcaacgcaac
     1321 cggttttttt caggagaaaa gccccgcccg taccgagttt aaggacctga tggaaagcta
     1381 tcgctactat aatcccaaga tccgatatga gttagtggac ccggacaagg atccggggac
     1441 ggcccgccgg tatggcatca cccagccgga caccgtggtg ctggaaagcg gggcccagga
     1501 tatccgagtc cgcaacatct cagagcagga attgaccagc gcgatcatcc gtgtgagccg
     1561 ggaaggcaag cgggtgatct atttcctgga ggggcatgga gagcatgggt tggacgatgg
     1621 ggatcgggct ggatattcca gcgcgaaaga gggattggag cgacagggat ttgaggtgaa
     1681 gagcctctta ctccttgagc aacctaaagt accttccgat gcgacgatct tggtcctggc
     1741 cggatctcag cggcccatct taaaagagga acaaggcgtc ctgagtgcct atctgaaagc
     1801 aggtggacaa ctccttgtga tgctggatcc g
//
LOCUS       DATMKH010000716         1033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_2564026, whole genome shotgun sequence.
ACCESSION   DATMKH010000716 DATMKH010000000
VERSION     DATMKH010000716.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2564026"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="VNV63_08485"
     CDS             complement(<1..444)
                     /locus_tag="VNV63_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXC62698.1"
                     /translation="MRAIFLIHLRQVIRDKDWVYPVFLLLLCTLLGQYLAPTDDDPTL
                     VYSTRTQAIWLCAWACSIFWVSFVAARIGSIQRQYHLREFWKSLGVPDLGYFFALFAI
                     PNLLNAALFFSAAFLSIFTGKQFDVPLGDWILVNFQAAVFAVLAQA"
     gene            complement(441..>1033)
                     /locus_tag="VNV63_08490"
     CDS             complement(441..>1033)
                     /locus_tag="VNV63_08490"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXC62699.1"
                     /translation="QILFEAGQITLLLGLNGQGKTTLMKTLAGLISPLAGSVARTPML
                     YLSDDVSFPARLTPSEIVQCLDPGRKYRALGSEMLAGLEIEDKRYGLLSKGNRQKARV
                     IFAEIITRARKVNFLGIDEPFAGLDFQARDYLVNRWLENVNRERHLLVSMHPSEIPVA
                     PSQILLVSEGKIWTVPPATSWSEIRVLLQRPKLILV"
BASE COUNT          258 a          283 c          287 g          205 t
ORIGIN      
        1 tgcctgcgcc agcacggcaa agaccgccgc ctgaaaattg accaggatcc agtcgcccag
       61 cgggacatcg aactgttttc ccgtgaaaat gctgagaaaa gcggcagaga aaaacagggc
      121 ggcgttcagg agatttggga tcgcgaacag tgcgaagaaa tacccgagat cggggacccc
      181 caggcttttc cagaactcgc gcaagtggta ctgccgttgt atcgagccaa ttcttgccgc
      241 cacgaaactg acccagaaga tcgagcacgc ccaggcacac agccagatcg cctgtgtgcg
      301 cgtggaataa accagggtcg gatcgtcgtc ggtcggtgca aggtattgtc ccagcagcgt
      361 acaaagaaga aggagaaaca ccggataaac ccaatccttg tcgcgaatca cttgtctgag
      421 atgaatgaga aagattgctc tcataccaaa ataagcttgg gcctttgcag cagaactctg
      481 atctcggacc aggatgtcgc gggcggcacc gtccaaattt tcccttcaga gaccaggagg
      541 atttgggaag gcgccaccgg gatctccgaa ggatgcatgc tgacgagcag atgacgctcg
      601 cgattcacat tctccagcca gcggttgacg agataatctc ttgcctggaa atccagaccg
      661 gcgaacggtt catcgatccc gaggaaattg acctttcgcg ccctggtgat gatttcggca
      721 aagatgacac gcgctttctg gcgattgccc ttggaaagca ggccatacct tttgtcttcg
      781 atttccaatc cggccagcat ttcgctcccc aaggcccggt atttcctccc tgggtcgaga
      841 cattgaacaa tctcgcttgg ggtgaggcgg gccgggaaac tgacgtcgtc agacaaatag
      901 agcatcgggg tccgggcaac gcttcccgcc agaggggaga tcaacccggc cagagtcttc
      961 attaaagtcg ttttcccctg accattgagc ccaagcagaa gggtgatttg acccgcttca
     1021 aagaggatct gct
//
LOCUS       DATMKH010000717         1911 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospiria bacterium isolate SMAG_U12766
            k141_4805465, whole genome shotgun sequence.
ACCESSION   DATMKH010000717 DATMKH010000000
VERSION     DATMKH010000717.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943374
            Sequence Read Archive: SRR7094834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiria bacterium (soil metagenome)
  ORGANISM  Nitrospiria bacterium
            Bacteria; Nitrospirota; Nitrospiria.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:59:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Nitrospiria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4805465"
                     /isolate="SMAG_U12766"
                     /isolation_source="soil"
                     /db_xref="taxon:2306052"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="VNV63_08495"
     CDS             complement(<1..392)
                     /locus_tag="VNV63_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HXC62700.1"
                     /translation="MSSLTLWQSLARKSFGYWLIFPVLLVILGLNLYPTIQGILTSFT
                     NQSLIDPGHSVYLGLENYFKLLKEPVFQVALLHSLELTFFAVALQVLLGLMMAHLLIQ
                     KIPGIHFFRSVAMITWVLPIIASVVMFR"
     gene            complement(627..>1911)
                     /locus_tag="VNV63_08500"
     CDS             complement(627..>1911)
                     /locus_tag="VNV63_08500"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HXC62701.1"
                     /translation="KILPLALALVATSSAGFAKELDLWFTPMSSEGPMKLPLTQWLKD
                     KLPKELPGVTVNNNFGPPVYQDGQQKFVVQGRKGKPDVIESVLEGMIAYQRAGLLDPL
                     DDLFDKWPDKDQFIPSTIKALTLNGKLYGVPYNTNVRVLLYRKDLFEKYQLTPPKTWD
                     DFLQDAATISSKESGVAGIGLTTKNGSVRTFQEFISFFFEVNNGENPYKYDEAGKKWV
                     MNTTPEKLAQVLKLYTDFFFAATPSAANQNTRGNDYQATDTDFVSGKSAMVPMGPWIY
                     SYRTSGDVAKKILEEETGVAPLPLPPGGVQATYLEVKPISINAFSKDKKDAWDLVKLL
                     TSKEFVATANRIEGVNPPRKDVAELPDFKNDWWEQAFIAQLPTGVALAPINWGLVIND
                     ITEALQKAIYKNATPEDAGKALYSTLEQRAKDNQL"
BASE COUNT          426 a          501 c          502 g          482 t
ORIGIN      
        1 aaccggaaca tgaccaccga ggcgatgatc ggcagaaccc acgtgatcat cgctacgctt
       61 cgaaaaaagt gaattccagg aattttctgg atcagaaggt gcgccatcat caggccgaga
      121 agaacctgga gtgcgacggc aaaaaaagtc agctccagag aatgcaacag cgccacttgg
      181 aaaaccggtt ccttcaggag cttaaaataa ttttcgagtc cgaggtatac ggaatggccg
      241 ggatcgatga gagactgatt ggtaaaagag gtcaggattc cctggatcgt cgggtaaagg
      301 ttcaacccga ggatcacaag aagcaccggg aaaataagcc agtacccgaa gctcttcctg
      361 gccagacttt gccaaagtgt gagtgagctc attccttggt ataccaatgt agcacaggcg
      421 ctagcctgtg ttcgccgcga tccacagacg tccttgggtc atgtgttcag attcgtctat
      481 cctaagacaa taagacatgc ctctcttaag cggacgcgct gcgtctgctc aaagagcttt
      541 ccagctggtc cgcctcagtt cttacacacc ctgcatctct aatccgcccc aagcataaac
      601 ctggggcgga aaccatttct tgcgggctag agctgattgt ctttggcgcg ctgctcgagc
      661 gtactgtaaa gcgccttgcc ggcatcttcc ggagttgcgt tcttgtaaat cgccttttgc
      721 agagcctcgg taatatcgtt aatgaccagg ccccagttga tcggcgccag ggccacgccg
      781 gtcggtagct gcgcgatgaa tgcttgttcc caccagtcgt ttttgaagtc tggcaattcc
      841 gcaacatctt ttctgggtgg attaacccct tcgatccggt tcgctgtagc gacgaactct
      901 ttgcttgtca aaagcttaac gaggtcccag gcatctttct tatctttgct gaaggcgttg
      961 atcgagattg gtttcacttc cagataggtc gcctggaccc ctcccggcgg gagagggagt
     1021 ggagctacgc cggtttcttc ttcaagaatt ttcttcgcaa cgtcgccgct cgtccgataa
     1081 ctgtaaatcc acggacccat cggtaccatc gcgctcttcc cggacacgaa atctgtgtcc
     1141 gttgcctgat agtcatttcc gcgtgtattc tggttcgccg cagacggcgt cgccgcgaaa
     1201 aagaaatcgg tgtacagctt caggacctga gccagttttt cgggcgtcgt attcatcacc
     1261 cattttttgc cggcttcatc gtatttgtag gggttttcac cgttgttcac ttcaaagaag
     1321 aacgaaatga attcctggaa cgttcggaca cttccgtttt ttgtcgttag ccctatgccc
     1381 gcgacgccgg attccttgga ggaaattgtt gctgcgtcct ggaggaagtc gtcccaagtt
     1441 tttggaggag tgagctggta tttctcgaac agatctttgc gataaagcag gacgcgaacg
     1501 tttgtgttgt acgggacgcc gtagagtttt ccattcagcg tcagcgcctt gatggtgctc
     1561 gggatgaact ggtctttgtc cggccactta tcgaaaagat catcaagcgg gtcaagcaat
     1621 ccggctcgct gatacgcaat catcccttca agcaccgact cgatcacatc cggtttacct
     1681 ttccggcctt ggacgacaaa cttttgctgg ccatcctgat agacgggagg tccgaagttg
     1741 ttgttgacgg tcacaccagg gagctccttt gggagtttat ctttcaacca ctgggtcagg
     1801 ggcaacttca taggcccttc ggatgacatc ggggtgaacc aaagatcgag ttctttggcg
     1861 aacccggccg acgatgttgc caccagcgcc agtgcgagcg gcaaaatttt g
//
